Homology
BLAST of Spg023566 vs. NCBI nr
Match:
XP_038879261.1 (phospholipase D alpha 4 [Benincasa hispida])
HSP 1 Score: 1523.8 bits (3944), Expect = 0.0e+00
Identity = 711/761 (93.43%), Postives = 740/761 (97.24%), Query Frame = 0
Query: 1 MAMEGKHKFFHGTLEVTVFHATAYTPPSPLDCLFAGGKRSYVTIKIDNKEVAQTSHEYDR 60
MAMEGK KFFHGTLEVTVFHATAYTPPSPLDC+F+GGKRSYVTIKIDNKEVAQTSHE DR
Sbjct: 1 MAMEGKQKFFHGTLEVTVFHATAYTPPSPLDCIFSGGKRSYVTIKIDNKEVAQTSHEQDR 60
Query: 61 VWNQTFRVLCAYPLTSTVAITMKTSRSVLGRFQIQAQQILKEASFINGFFPLLMENGKPS 120
VWNQTFRVLCA+PLTSTV ITMKTSRSVLG+F IQAQQILKEASFINGFFPLLMENGKPS
Sbjct: 61 VWNQTFRVLCAHPLTSTVTITMKTSRSVLGKFYIQAQQILKEASFINGFFPLLMENGKPS 120
Query: 121 PELRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHVPTFRPP 180
PEL+LRFMLWFKPA+YELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHH+PTF+PP
Sbjct: 121 PELKLRFMLWFKPAIYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPP 180
Query: 181 FHGSNAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ 240
FHGS+ PRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDS TDIPYALGVKLGELLKQ
Sbjct: 181 FHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSHTDIPYALGVKLGELLKQ 240
Query: 241 KAEEGVAVRLLIWDDETSLPIIKNAGIMKTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300
KAEEGVAVR+LIWDDETSLPIIKNAGIM THDEDARAYFLHSKVICRLCPKLHPMSPPIF
Sbjct: 241 KAEEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300
Query: 301 SHHQKTIIVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCYDFYQTS 360
SHHQKTI VDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLN ESHC DFYQTS
Sbjct: 301 SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNRESHCSDFYQTS 360
Query: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420
ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL
Sbjct: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420
Query: 421 ESNTSTQKDWHVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 480
ES+T+ QKDW+VQVFRSIDHLSASQVFRN+T+ERTIHEAYVEAIRRAERFIYIENQYFIG
Sbjct: 421 ESSTNPQKDWNVQVFRSIDHLSASQVFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIG 480
Query: 481 GCHLWDKDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPESESVEDMLHW 540
GCHLWDKDQHCGCTNLIPIEIALKV NKIKARERF VYIVIPMWPEGPPESESVEDMLHW
Sbjct: 481 GCHLWDKDQHCGCTNLIPIEIALKVVNKIKARERFTVYIVIPMWPEGPPESESVEDMLHW 540
Query: 541 TRQTMKMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEETKWDFVPPHSPQHATQYWNA 600
TRQTM MMYRLIGEAIQETGEKAHPRDYLNFFCLANREEE KWDF+PP SPQHATQYWN+
Sbjct: 541 TRQTMAMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEEGKWDFIPPQSPQHATQYWNS 600
Query: 601 QQHRRFMVYVHSKVMIVDDSYILIGSANVNQRSMDGERDTEIAIGCYQLENDGEELPNGR 660
QQHRRFM+YVHSKVMIVDD YILIGSANVNQRSMDG+RDTEIAIGCYQLENDGEE PNGR
Sbjct: 601 QQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGDRDTEIAIGCYQLENDGEEFPNGR 660
Query: 661 DISTFRLSLWYEHTQRFEDLFLQPENLQCVQRVRSIADESWEIYSGDEIADMKGVHLVTY 720
DISTFRLSLWYEHTQRFE++FL PE+L+CVQRVRSI D+SWEIYSG+E+ DMKGVHLVTY
Sbjct: 661 DISTFRLSLWYEHTQRFEEVFLNPESLKCVQRVRSIGDKSWEIYSGEEVEDMKGVHLVTY 720
Query: 721 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMLLPPIFTT 762
PVKVK+DGS+EDLEENGGHFPDTKCPIKGRRSM+LPPIFTT
Sbjct: 721 PVKVKEDGSMEDLEENGGHFPDTKCPIKGRRSMMLPPIFTT 761
BLAST of Spg023566 vs. NCBI nr
Match:
XP_008447602.1 (PREDICTED: phospholipase D alpha 4 [Cucumis melo] >KAA0051141.1 phospholipase D alpha 4 [Cucumis melo var. makuwa] >TYK03757.1 phospholipase D alpha 4 [Cucumis melo var. makuwa])
HSP 1 Score: 1511.5 bits (3912), Expect = 0.0e+00
Identity = 705/761 (92.64%), Postives = 739/761 (97.11%), Query Frame = 0
Query: 1 MAMEGKHKFFHGTLEVTVFHATAYTPPSPLDCLFAGGKRSYVTIKIDNKEVAQTSHEYDR 60
MAME K KFFHG LEVTVFHATAYTPPSPLDCLFAGGKRSYVTIKIDNKEVAQTSHE+DR
Sbjct: 1 MAMEVKQKFFHGMLEVTVFHATAYTPPSPLDCLFAGGKRSYVTIKIDNKEVAQTSHEHDR 60
Query: 61 VWNQTFRVLCAYPLTSTVAITMKTSRSVLGRFQIQAQQILKEASFINGFFPLLMENGKPS 120
VWNQTFRVLCAYPLTST+ IT+KT+RSVLG+F IQAQQILKEASFINGFFPLLMENGKPS
Sbjct: 61 VWNQTFRVLCAYPLTSTITITLKTARSVLGKFYIQAQQILKEASFINGFFPLLMENGKPS 120
Query: 121 PELRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHVPTFRPP 180
PEL+LRFMLWFKPAVYELSWKKML NGEYKGLRNATFPLRSNCHVTLYQDAHH+PTF+PP
Sbjct: 121 PELKLRFMLWFKPAVYELSWKKMLANGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPP 180
Query: 181 FHGSNAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ 240
FHGS+ PRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ
Sbjct: 181 FHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ 240
Query: 241 KAEEGVAVRLLIWDDETSLPIIKNAGIMKTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300
KAEEGVAVR+LIWDDETSLPIIKNAGIM THDEDARAYFLHSKVICRLCPKLHPMSPPIF
Sbjct: 241 KAEEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300
Query: 301 SHHQKTIIVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCYDFYQTS 360
SHHQK IIVDAQTHINA+NREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHC DFYQTS
Sbjct: 301 SHHQKIIIVDAQTHINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTS 360
Query: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420
ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL
Sbjct: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420
Query: 421 ESNTSTQKDWHVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 480
ESNT+ QKDW+VQVFRSIDHLSASQ+FRN+T+ERTIHEAYVEAIRRAERFIYIENQYFIG
Sbjct: 421 ESNTNPQKDWNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIG 480
Query: 481 GCHLWDKDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPESESVEDMLHW 540
GCH WD+D+HCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEG PESESVEDMLHW
Sbjct: 481 GCHFWDRDRHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPESESVEDMLHW 540
Query: 541 TRQTMKMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEETKWDFVPPHSPQHATQYWNA 600
TRQTM MMYRLIGEAIQETGEKAHPRDYLNFFCLANREEE KWDF+PPHSPQHATQYWNA
Sbjct: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQYWNA 600
Query: 601 QQHRRFMVYVHSKVMIVDDSYILIGSANVNQRSMDGERDTEIAIGCYQLENDGEELPNGR 660
QQHRRFM+YVHSKVMIVDD YILIGSANVNQRSMDGERDTEIAIGCYQ+ENDGEELPNGR
Sbjct: 601 QQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQIENDGEELPNGR 660
Query: 661 DISTFRLSLWYEHTQRFEDLFLQPENLQCVQRVRSIADESWEIYSGDEIADMKGVHLVTY 720
DIS FRLSLWYEHT+ FE++FL PE+L+CVQRVRSI D+SW+IYSG+E+ DMKGVHLVTY
Sbjct: 661 DISRFRLSLWYEHTRGFEEVFLNPESLKCVQRVRSIGDKSWKIYSGEEVEDMKGVHLVTY 720
Query: 721 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMLLPPIFTT 762
PVKVK+DG++EDLEENGGHFPDTKC IKGRRSM+LPPIFTT
Sbjct: 721 PVKVKEDGTMEDLEENGGHFPDTKCAIKGRRSMMLPPIFTT 761
BLAST of Spg023566 vs. NCBI nr
Match:
XP_023525777.1 (phospholipase D alpha 4 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1510.4 bits (3909), Expect = 0.0e+00
Identity = 707/761 (92.90%), Postives = 740/761 (97.24%), Query Frame = 0
Query: 1 MAMEGKHKFFHGTLEVTVFHATAYTPPSPLDCLFAGGKRSYVTIKIDNKEVAQTSHEYDR 60
M + GKHKFFHGTLEVTVFHAT YTP SPLDC+F+GGK+SYVTIKI+N EVA+T+HEYDR
Sbjct: 61 MTVHGKHKFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR 120
Query: 61 VWNQTFRVLCAYPLTSTVAITMKTSRSVLGRFQIQAQQILKEASFINGFFPLLMENGKPS 120
VWNQTFRVLCA+PLTST+ ITM+TSRSVLGRFQIQAQQILKE+SFINGFFPLLMENGKPS
Sbjct: 121 VWNQTFRVLCAHPLTSTITITMRTSRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS 180
Query: 121 PELRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHVPTFRPP 180
PELRLRFMLWF+PAVYELSWKK+L NGEYKGLRNATFPLRSNCHVTLYQDAHHV TF+PP
Sbjct: 181 PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCHVTLYQDAHHVSTFQPP 240
Query: 181 FHGSNAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ 240
FHGS+APRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRD QTDIPYALGVKLGELLKQ
Sbjct: 241 FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTDIPYALGVKLGELLKQ 300
Query: 241 KAEEGVAVRLLIWDDETSLPIIKNAGIMKTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300
KAEEGVAVRL+IWDDETSLP+IKNAGIMKTHDEDARAYF +SKV+CRLCPKLHPMSPPIF
Sbjct: 301 KAEEGVAVRLMIWDDETSLPVIKNAGIMKTHDEDARAYFSNSKVVCRLCPKLHPMSPPIF 360
Query: 301 SHHQKTIIVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCYDFYQTS 360
SHHQKTI VDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHC DFYQTS
Sbjct: 361 SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS 420
Query: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420
ISGAKLQKGGPREPWHDVHA VTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMP L
Sbjct: 421 ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL 480
Query: 421 ESNTSTQKDWHVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 480
ESNT+ QKDW+VQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG
Sbjct: 481 ESNTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 540
Query: 481 GCHLWDKDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPESESVEDMLHW 540
GCHLWDKDQHCGCTNLIPIEIALKVA KI+ARE+FAVY+VIPMWPEGPPESESVEDMLHW
Sbjct: 541 GCHLWDKDQHCGCTNLIPIEIALKVATKIRAREKFAVYVVIPMWPEGPPESESVEDMLHW 600
Query: 541 TRQTMKMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEETKWDFVPPHSPQHATQYWNA 600
TRQTM MMYRLIGEAIQETGEKAHPRDYLNFFCLANREEE KWDFVPPHSPQHAT+YW+A
Sbjct: 601 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA 660
Query: 601 QQHRRFMVYVHSKVMIVDDSYILIGSANVNQRSMDGERDTEIAIGCYQLENDGEELPNGR 660
QQHRRFMVYVHSKVMIVDD YILIGSANVNQRSMDGERDTEIAIGCYQLENDG+ELPNGR
Sbjct: 661 QQHRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR 720
Query: 661 DISTFRLSLWYEHTQRFEDLFLQPENLQCVQRVRSIADESWEIYSGDEIADMKGVHLVTY 720
DISTFRLSLWYEHTQRFE++FL PENLQCV+RVRSIADESW+IYSG+E+ADMKGVHLVTY
Sbjct: 721 DISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTY 780
Query: 721 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMLLPPIFTT 762
PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSM LPPIFTT
Sbjct: 781 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 821
BLAST of Spg023566 vs. NCBI nr
Match:
KAG6606722.1 (Phospholipase D alpha 4, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1509.2 bits (3906), Expect = 0.0e+00
Identity = 706/761 (92.77%), Postives = 740/761 (97.24%), Query Frame = 0
Query: 1 MAMEGKHKFFHGTLEVTVFHATAYTPPSPLDCLFAGGKRSYVTIKIDNKEVAQTSHEYDR 60
M ++GKHKFFHGTLEVTVFHAT YTP SPLDC+F+GGK+SYVTIKI+N EVA+T+HEYDR
Sbjct: 61 MTLQGKHKFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR 120
Query: 61 VWNQTFRVLCAYPLTSTVAITMKTSRSVLGRFQIQAQQILKEASFINGFFPLLMENGKPS 120
VWNQTFRVLCA+PLTST+ ITM+T+RSVLGRFQIQAQQILKE+SFINGFFPLLMENGKPS
Sbjct: 121 VWNQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS 180
Query: 121 PELRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHVPTFRPP 180
PELRLRFMLWF+PAVYELSWKK+L NGEYKGLRNATFPLRSNCHVTLYQDAHHV TF+PP
Sbjct: 181 PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCHVTLYQDAHHVSTFQPP 240
Query: 181 FHGSNAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ 240
FHGS+APRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRD QT IPYALGVKLGELLKQ
Sbjct: 241 FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTHIPYALGVKLGELLKQ 300
Query: 241 KAEEGVAVRLLIWDDETSLPIIKNAGIMKTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300
KAEEGVAVRL+IWDDETSLPIIKNAGIMKTHDEDARAYF +SKV+CRLCPKLHPMSPPIF
Sbjct: 301 KAEEGVAVRLMIWDDETSLPIIKNAGIMKTHDEDARAYFSNSKVVCRLCPKLHPMSPPIF 360
Query: 301 SHHQKTIIVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCYDFYQTS 360
SHHQKTI VDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHC DFYQTS
Sbjct: 361 SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS 420
Query: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420
ISGAKLQKGGPREPWHDVHA VTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMP L
Sbjct: 421 ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL 480
Query: 421 ESNTSTQKDWHVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 480
ESNT+ QKDW+VQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG
Sbjct: 481 ESNTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 540
Query: 481 GCHLWDKDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPESESVEDMLHW 540
GCHLWDKDQHCGCTNLIPIEIALKVANKIKARE+FAVY+VIPMWPEGPPESESVEDMLHW
Sbjct: 541 GCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHW 600
Query: 541 TRQTMKMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEETKWDFVPPHSPQHATQYWNA 600
TRQTM MMYRLIGEAIQETGEKAHPRDYLNFFCLANREEE KWDFVPPHSPQHAT+YW+A
Sbjct: 601 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA 660
Query: 601 QQHRRFMVYVHSKVMIVDDSYILIGSANVNQRSMDGERDTEIAIGCYQLENDGEELPNGR 660
Q HRRFMVYVHSKVMIVDD YILIGSANVNQRSMDGERDTEIAIGCYQLENDG+ELPNGR
Sbjct: 661 QHHRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR 720
Query: 661 DISTFRLSLWYEHTQRFEDLFLQPENLQCVQRVRSIADESWEIYSGDEIADMKGVHLVTY 720
DISTFRLSLWYEHTQRFE++FL PENLQCV+R+RSIADESW+IYSG+E+ADMKGVHLVTY
Sbjct: 721 DISTFRLSLWYEHTQRFEEVFLNPENLQCVERIRSIADESWKIYSGEEVADMKGVHLVTY 780
Query: 721 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMLLPPIFTT 762
PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSM LPPIFTT
Sbjct: 781 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 821
BLAST of Spg023566 vs. NCBI nr
Match:
KAG7036437.1 (Phospholipase D alpha 4 [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1509.2 bits (3906), Expect = 0.0e+00
Identity = 707/761 (92.90%), Postives = 740/761 (97.24%), Query Frame = 0
Query: 1 MAMEGKHKFFHGTLEVTVFHATAYTPPSPLDCLFAGGKRSYVTIKIDNKEVAQTSHEYDR 60
M ++GKHKFFHGTLEVTVFHAT YTP SPLDC+F+GGK+SYVTIKI+N EVA+T+HEYDR
Sbjct: 1 MTVQGKHKFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR 60
Query: 61 VWNQTFRVLCAYPLTSTVAITMKTSRSVLGRFQIQAQQILKEASFINGFFPLLMENGKPS 120
VWNQTFRVLCA+PLTST+ ITM+T+RSVLGRFQIQAQQILKE+SFINGFFPLLMENGKPS
Sbjct: 61 VWNQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS 120
Query: 121 PELRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHVPTFRPP 180
PELRLRFMLWF+PAVYELSWKK+L NGEYKGLRNATFPLRSNCHVTLYQDAHHV TF+PP
Sbjct: 121 PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCHVTLYQDAHHVSTFQPP 180
Query: 181 FHGSNAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ 240
FHGS+APRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRD QT IPYALGVKLGELLKQ
Sbjct: 181 FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTHIPYALGVKLGELLKQ 240
Query: 241 KAEEGVAVRLLIWDDETSLPIIKNAGIMKTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300
KAEEGVAVRL+IWDDETSLPIIKNAGIMKTHDEDARAYF +SKV+CRLCPKLHPMSPPIF
Sbjct: 241 KAEEGVAVRLMIWDDETSLPIIKNAGIMKTHDEDARAYFSNSKVVCRLCPKLHPMSPPIF 300
Query: 301 SHHQKTIIVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCYDFYQTS 360
SHHQKTI VDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHC DFYQTS
Sbjct: 301 SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS 360
Query: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420
ISGAKLQKGGPREPWHDVHA VTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMP L
Sbjct: 361 ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL 420
Query: 421 ESNTSTQKDWHVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 480
ESNT+ QKDW+VQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG
Sbjct: 421 ESNTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 480
Query: 481 GCHLWDKDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPESESVEDMLHW 540
GCHLWDKDQHCGCTNLIPIEIALKVANKIKARE+FAVY+VIPMWPEGPPESESVEDMLHW
Sbjct: 481 GCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHW 540
Query: 541 TRQTMKMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEETKWDFVPPHSPQHATQYWNA 600
TRQTM MMYRLIGEAIQETGEKAHPRDYLNFFCLANREEE KWDFVPPHSPQHAT+YW+A
Sbjct: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA 600
Query: 601 QQHRRFMVYVHSKVMIVDDSYILIGSANVNQRSMDGERDTEIAIGCYQLENDGEELPNGR 660
Q HRRFMVYVHSKVMIVDD YILIGSANVNQRSMDGERDTEIAIGCYQLENDG+ELPNGR
Sbjct: 601 QHHRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR 660
Query: 661 DISTFRLSLWYEHTQRFEDLFLQPENLQCVQRVRSIADESWEIYSGDEIADMKGVHLVTY 720
DISTFRLSLWYEHTQRFE++FL PENLQCV+RVRSIADESW+IYSG+E+ADMKGVHLVTY
Sbjct: 661 DISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTY 720
Query: 721 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMLLPPIFTT 762
PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSM LPPIFTT
Sbjct: 721 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 761
BLAST of Spg023566 vs. ExPASy Swiss-Prot
Match:
Q9C888 (Phospholipase D alpha 4 OS=Arabidopsis thaliana OX=3702 GN=PLDALPHA4 PE=2 SV=1)
HSP 1 Score: 980.3 bits (2533), Expect = 1.2e-284
Identity = 463/776 (59.66%), Postives = 600/776 (77.32%), Query Frame = 0
Query: 1 MAMEGKHKFFHGTLEVTVFHATAYTPPSPLDCLFAGGKRSYVTIKIDNKEVAQTSHEYDR 60
M +E + K+FHGTLE+T+F AT ++PP P +C+ K +YVTIKI+ K+VA+TS EYDR
Sbjct: 1 MELEEQKKYFHGTLEITIFDATPFSPPFPFNCICTKPKAAYVTIKINKKKVAKTSSEYDR 60
Query: 61 VWNQTFRVLCAYPLT-STVAITMKTSRSVLGRFQIQAQQIL-KEASFINGFFPLLMENGK 120
+WNQTF++LCA+P+T +T+ IT+KT SVLGRF+I A+QIL ++ INGFFPL+ +NG
Sbjct: 61 IWNQTFQILCAHPVTDTTITITLKTRCSVLGRFRISAEQILTSNSAVINGFFPLIADNGS 120
Query: 121 PSPELRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHVPTFR 180
L+L+ ++WF+PA E W + L ++G+RNA+FP RSNC V LYQDAHH TF
Sbjct: 121 TKRNLKLKCLMWFRPAYLEPGWCRALEEASFQGIRNASFPQRSNCRVVLYQDAHHKATFD 180
Query: 181 P-----PFHGSNAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVK 240
P PF+ R LWEDVYKAI++A+HLVYIAGW+ NP +VLVRD++T+IP+A+GV
Sbjct: 181 PRVDDVPFNA----RNLWEDVYKAIESARHLVYIAGWALNPNLVLVRDNETEIPHAVGVT 240
Query: 241 LGELLKQKAEEGVAVRLLIWDDETSLPIIKNAGIMKTHDEDARAYFLHSKVICRLCPKLH 300
+GELLK+K+EEGVAVR+++W+DETSLP+IKN G+M+T+ E A AYF ++ V+CRLCP+LH
Sbjct: 241 VGELLKRKSEEGVAVRVMLWNDETSLPMIKNKGVMRTNVERALAYFRNTNVVCRLCPRLH 300
Query: 301 PMSPPIFSHHQKTIIVDAQ-THINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESH 360
P F+HHQKTI +D + T+ + + REIMSF+GG DLCDGRYDTE+HSLF TL TE+
Sbjct: 301 KKLPTAFAHHQKTITLDTRVTNSSTKEREIMSFLGGFDLCDGRYDTEEHSLFRTLGTEA- 360
Query: 361 CYDFYQTSISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSI 420
DFYQTS++GAKL +GGPREPWHD H V G AAWD+L NFEQRWTKQ + S+LV TS
Sbjct: 361 --DFYQTSVAGAKLSRGGPREPWHDCHVSVVGGAAWDVLKNFEQRWTKQCNPSVLVNTSG 420
Query: 421 LLKLMPQLESNTSTQKDWHVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIY 480
+ L+ + W+VQV RSIDH+SA+++ R L VE+++H+ YV AIR+AERFIY
Sbjct: 421 IRNLVNLTGPTEENNRKWNVQVLRSIDHISATEMPRGLPVEKSVHDGYVAAIRKAERFIY 480
Query: 481 IENQYFIGGCHLWD--KDQHC-GCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPP 540
IENQYF+G C W+ D+ C GCTNLIP+EIALK+A KI+ARERFAVYIVIPMWPEGPP
Sbjct: 481 IENQYFMGSCDHWESKNDKICSGCTNLIPVEIALKIAAKIRARERFAVYIVIPMWPEGPP 540
Query: 541 ESESVEDMLHWTRQTMKMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEETKWDFVPPH 600
ESE+VE++LHWTR+TM MMY++IGEAI E G+K+HPRDYLNFFCLANREE+ +F
Sbjct: 541 ESETVEEILHWTRETMSMMYQIIGEAIWEVGDKSHPRDYLNFFCLANREEKRDGEFEAVS 600
Query: 601 SPQHATQYWNAQQHRRFMVYVHSKVMIVDDSYILIGSANVNQRSMDGERDTEIAIGCYQL 660
SP T YWNAQ++RRFMVYVHSK+MIVDD+YILIGSAN+NQRSMDG RDTEIAIGCYQ
Sbjct: 601 SPHQKTHYWNAQRNRRFMVYVHSKLMIVDDTYILIGSANINQRSMDGCRDTEIAIGCYQT 660
Query: 661 ENDGEELPNGRDISTFRLSLWYEHTQ---RFEDL-FLQPENLQCVQRVRSIADESWEIYS 720
+ N +I +RLSLWYEHT +DL +PE+L+CV+ +R+I ++ WEIYS
Sbjct: 661 NTN-----NTNEIQAYRLSLWYEHTGGKITADDLSSSEPESLECVRGLRTIGEQMWEIYS 720
Query: 721 GDEIADMKGVHLVTYPVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMLLPPIFTT 762
GD++ DM G+HLV YP+ V DG++E++ + G FPDTK +KG+RS + PP+ TT
Sbjct: 721 GDKVVDMLGIHLVAYPISVTGDGAVEEVGD--GCFPDTKTLVKGKRSKMFPPVLTT 762
BLAST of Spg023566 vs. ExPASy Swiss-Prot
Match:
Q43270 (Phospholipase D alpha 1 OS=Zea mays OX=4577 GN=PLD1 PE=2 SV=1)
HSP 1 Score: 626.3 bits (1614), Expect = 4.5e-178
Identity = 333/810 (41.11%), Postives = 477/810 (58.89%), Query Frame = 0
Query: 11 HGTLEVTVFHATAYTPP------------------SPLDCLFAGGKRSYVTIKIDNKEVA 70
HGTL T+F A + + P + G + Y T+ ++ V
Sbjct: 7 HGTLHATIFEAESLSNPHRATGGAPKFIRKLVEGIEDTVGVGKGATKIYATVDLEKARVG 66
Query: 71 QT----SHEYDRVWNQTFRVLCAY---PLTSTVAITMKTSRSVLGRFQIQAQQILKEASF 130
+T + + W ++F + CA+ + TV I S++GR + Q +L
Sbjct: 67 RTRMISNEPVNPRWYESFHIYCAHMAADVIFTVKIDNSIGASLIGRAYLAVQDLL-GGEE 126
Query: 131 INGFFPLLMENGKPSPELRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHV 190
I+ + + EN +P + ++ L + + +W + + + +Y G+ F R C V
Sbjct: 127 IDKWLEISDENREPVGDSKIHVKLQYFDVGKDRNWARGVRSTKYPGVPYTFFSQRQGCKV 186
Query: 191 TLYQDAHHVPTFRPPFH---GSN-APRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRD 250
TLYQDAH F P G N P R WED++ AI A+HL+YI GWS ++ LVRD
Sbjct: 187 TLYQDAHVPDNFVPRIQLADGKNYEPHRCWEDIFDAISKAQHLIYITGWSVYTEITLVRD 246
Query: 251 SQTDIPYALGVKLGELLKQKAEEGVAVRLLIWDDETSLPIIKNAGIMKTHDEDARAYFLH 310
+ P V LGELLK+KA EGV V +L+WDD TS+ ++K G+M THDE+ YF
Sbjct: 247 TNRPKPGG-DVTLGELLKRKASEGVRVLMLVWDDRTSVGLLKKDGLMATHDEETANYFHG 306
Query: 311 SKVICRLCPKLHPMS---------PPIFSHHQKTIIVDAQ-THINARNREIMSFIGGLDL 370
+ V C LCP+ S +F+HHQK ++VD + + ++ R I+SFIGG+DL
Sbjct: 307 TDVNCVLCPRNPDDSGSFVQDLQISTMFTHHQKIVVVDHEMPNQGSQQRRIVSFIGGIDL 366
Query: 371 CDGRYDTEQHSLFHTLNTESHCYDFYQTSISGAKLQKGGPREPWHDVHACVTGEAAWDIL 430
CDGRYDT+ HSLF TL+T H DF+Q + G ++KGGPREPWHD+H+ + G AWD+L
Sbjct: 367 CDGRYDTQYHSLFRTLDTVHH-DDFHQPNFEGGSIKKGGPREPWHDIHSRLEGPIAWDVL 426
Query: 431 TNFEQRWTKQSDASLLVPTSIL--LKLMPQLESNTSTQKDWHVQVFRSIDHLSASQV--- 490
NFEQRW KQ LLV L + + P ++ W+VQ+FRSID +A
Sbjct: 427 YNFEQRWRKQGGKDLLVRLRDLPDIIIPPSPVMFPEDRETWNVQLFRSIDGGAAFGFPET 486
Query: 491 -----------FRNLTVERTIHEAYVEAIRRAERFIYIENQYFIGGCHLWD----KDQHC 550
++ ++R+I +AYV AIRRA+ FIYIENQYF+G + W K +
Sbjct: 487 PEEAARAGLVSGKDQIIDRSIQDAYVNAIRRAKNFIYIENQYFLGSSYGWKPEGIKPEEI 546
Query: 551 GCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPESESVEDMLHWTRQTMKMMYRL 610
G +LIP E++LK+ +KI+A ERF VY+V+PMWPEG PES SV+ +L W R+TM+MMY
Sbjct: 547 GALHLIPKELSLKIVSKIEAGERFTVYVVVPMWPEGVPESASVQAILDWQRRTMEMMYTD 606
Query: 611 IGEAIQETGEKAHPRDYLNFFCLANREEETKWDFVPPHSPQHATQYWNAQQHRRFMVYVH 670
I +A++ G +A+P+DYL FFCL NRE + + ++ P P+ T Y AQ+ RRFM+YVH
Sbjct: 607 IAQALEANGIEANPKDYLTFFCLGNREVKQEGEYEPEEHPEPDTDYIRAQEARRFMIYVH 666
Query: 671 SKVMIVDDSYILIGSANVNQRSMDGERDTEIAIGCYQLENDGEELPNGRDISTFRLSLWY 730
+K+MIVDD YI+IGSAN+NQRSMDG RD+EIA+G YQ + P I FR+SLWY
Sbjct: 667 TKMMIVDDEYIIIGSANINQRSMDGARDSEIAMGAYQPYHLATRQPARGQIHGFRMSLWY 726
Query: 731 EHTQRFEDLFLQPENLQCVQRVRSIADESWEIYSGDEIADMKGVHLVTYPVKVKQDGSIE 762
EH ED+F +PE+++CVQ+V +A++ W++YS D++ HL++YP+ V DGS+
Sbjct: 727 EHLGMLEDVFQRPESVECVQKVNEVAEKYWDLYSSDDLEQDLPGHLLSYPIGVTADGSVT 786
BLAST of Spg023566 vs. ExPASy Swiss-Prot
Match:
Q43007 (Phospholipase D alpha 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PLD1 PE=1 SV=2)
HSP 1 Score: 625.5 bits (1612), Expect = 7.6e-178
Identity = 336/811 (41.43%), Postives = 478/811 (58.94%), Query Frame = 0
Query: 11 HGTLEVTVFHATAYTPPSPLD------------------CLFAGGKRSYVTIKIDNKEVA 70
HGTL T+F A + + P + G + Y TI ++ V
Sbjct: 7 HGTLHATIFEAASLSNPHRASGSAPKFIRKFVEGIEDTVGVGKGATKVYSTIDLEKARVG 66
Query: 71 Q----TSHEYDRVWNQTFRVLCAYPLTSTVAITMKTSRSV----LGRFQIQAQQILKEAS 130
+ T+ + W ++F + CA+ + S V T+K + +GR + Q++L
Sbjct: 67 RTRMITNEPINPRWYESFHIYCAH-MASNVIFTVKIDNPIGATNIGRAYLPVQELL-NGE 126
Query: 131 FINGFFPLLMENGKPSPELRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCH 190
I+ + + N +P E ++ L + + +W + + + +Y G+ F R C
Sbjct: 127 EIDRWLDICDNNREPVGESKIHVKLQYFDVSKDRNWARGVRSTKYPGVPYTFFSQRQGCK 186
Query: 191 VTLYQDAHHVPTFRPPF---HGSN-APRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVR 250
VTLYQDAH F P G N P R WED++ AI NA+HL+YI GWS ++ LVR
Sbjct: 187 VTLYQDAHVPDNFIPKIPLADGKNYEPHRCWEDIFDAISNAQHLIYITGWSVYTEITLVR 246
Query: 251 DSQTDIPYALGVKLGELLKQKAEEGVAVRLLIWDDETSLPIIKNAGIMKTHDEDARAYFL 310
DS P V LGELLK+KA EGV V +L+WDD TS+ ++K G+M THDE+ YF
Sbjct: 247 DSNRPKPGG-DVTLGELLKKKASEGVRVLMLVWDDRTSVGLLKRDGLMATHDEETENYFH 306
Query: 311 HSKVICRLCPKLHPMSPPI---------FSHHQKTIIVDAQ-THINARNREIMSFIGGLD 370
S V C LCP+ S I F+HHQK ++VD + + ++ R I+SF+GGLD
Sbjct: 307 GSDVNCVLCPRNPDDSGSIVQDLSISTMFTHHQKIVVVDHELPNQGSQQRRIVSFVGGLD 366
Query: 371 LCDGRYDTEQHSLFHTLNTESHCYDFYQTSISGAKLQKGGPREPWHDVHACVTGEAAWDI 430
LCDGRYDT+ HSLF TL++ +H DF+Q + + A ++KGGPREPWHD+H+ + G AWD+
Sbjct: 367 LCDGRYDTQYHSLFRTLDS-THHDDFHQPNFATASIKKGGPREPWHDIHSRLEGPIAWDV 426
Query: 431 LTNFEQRWTKQSDASLLVPTSILLK--LMPQLESNTSTQKDWHVQVFRSIDHLSASQV-- 490
L NFEQRW KQ LL+ L + P ++ W+VQ+FRSID +A
Sbjct: 427 LYNFEQRWRKQGGKDLLLQLRDLSDTIIPPSPVMFPEDRETWNVQLFRSIDGGAAFGFPD 486
Query: 491 ------------FRNLTVERTIHEAYVEAIRRAERFIYIENQYFIGGCHLWD----KDQH 550
++ ++R+I +AY+ AIRRA+ FIYIENQYF+G + W K +
Sbjct: 487 TPEEAAKAGLVSGKDQIIDRSIQDAYIHAIRRAKNFIYIENQYFLGSSYAWKPEGIKPED 546
Query: 551 CGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPESESVEDMLHWTRQTMKMMYR 610
G +LIP E+ALKV +KI+A ERF VY+V+PMWPEG PES SV+ +L W R+TM+MMY
Sbjct: 547 IGALHLIPKELALKVVSKIEAGERFTVYVVVPMWPEGVPESGSVQAILDWQRRTMEMMYT 606
Query: 611 LIGEAIQETGEKAHPRDYLNFFCLANREEETKWDFVPPHSPQHATQYWNAQQHRRFMVYV 670
I EA+Q G +A+P+DYL FFCL NRE + ++ P P+ T Y AQ+ RRFM+YV
Sbjct: 607 DITEALQAKGIEANPKDYLTFFCLGNREVKQAGEYQPEEQPEADTDYSRAQEARRFMIYV 666
Query: 671 HSKVMIVDDSYILIGSANVNQRSMDGERDTEIAIGCYQLENDGEELPNGRDISTFRLSLW 730
H+K+MIVDD YI+IGSAN+NQRSMDG RD+EIA+G YQ + P I FR++LW
Sbjct: 667 HTKMMIVDDEYIIIGSANINQRSMDGARDSEIAMGGYQPYHLATRQPARGQIHGFRMALW 726
Query: 731 YEHTQRFEDLFLQPENLQCVQRVRSIADESWEIYSGDEIADMKGVHLVTYPVKVKQDGSI 762
YEH +D+F +PE+L+CVQ+V IA++ W++YS D++ HL++YP+ V DG +
Sbjct: 727 YEHLGMLDDVFQRPESLECVQKVNRIAEKYWDMYSSDDLQQDLPGHLLSYPIGVASDGVV 786
BLAST of Spg023566 vs. ExPASy Swiss-Prot
Match:
P93400 (Phospholipase D alpha 1 OS=Nicotiana tabacum OX=4097 GN=PLD1 PE=2 SV=2)
HSP 1 Score: 601.7 bits (1550), Expect = 1.2e-170
Identity = 316/765 (41.31%), Postives = 459/765 (60.00%), Query Frame = 0
Query: 41 YVTIKIDNKEVAQT----SHEYDRVWNQTFRVLCAYPLTSTVAITMKTSR----SVLGRF 100
Y T+ ++ V +T + + W ++F + CA+ + S V T+K +++GR
Sbjct: 50 YATVDLEKARVGRTRKIKNEPNNPRWYESFHIYCAH-MASNVIFTVKDDNPIGATLIGRA 109
Query: 101 QIQAQQILKEASFINGFFPLLMENGKPSPE-LRLRFMLWFKPAVYELSWKKMLGNGEYKG 160
+ +++L E I+ + +L P E ++ L F + +W++ + + +Y G
Sbjct: 110 YVPVEELL-EGEEIDKWVEILDREMNPIAEGSKIHVKLQFFDVSRDPNWERGIRSSKYPG 169
Query: 161 LRNATFPLRSNCHVTLYQDAHHVPTFRP--PFHGSN--APRRLWEDVYKAIDNAKHLVYI 220
+ F R+ C V+LYQDAH F P P G P R WED++ AI NAKHL+YI
Sbjct: 170 VPYTFFAQRTGCRVSLYQDAHVPDNFIPKIPLSGGKYYEPHRCWEDIFDAIINAKHLIYI 229
Query: 221 AGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQKAEEGVAVRLLIWDDETSLPIIKNAGI 280
GWS ++ LVRDS+ P + LGELLK+KA EGV V +L+WDD TS+ ++K G+
Sbjct: 230 TGWSVYTEITLVRDSRRQKPGG-DITLGELLKKKASEGVKVLMLVWDDRTSVGLLKKDGL 289
Query: 281 MKTHDEDARAYFLHSKVICRLCPKLHPMSPPI---------FSHHQKTIIVDAQTHI-NA 340
M THD++ +F ++V C LCP+ I F+HHQK ++VD++ +
Sbjct: 290 MATHDQETEQFFQGTEVNCVLCPRNPDDGGSIVQSLQIGTMFTHHQKIVVVDSELPSGES 349
Query: 341 RNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCYDFYQTSISGAKLQKGGPREPWHD 400
R I+SF+GG+DLCDGRYDT HSLF TL+T +H DF+Q + + KGGPREPWHD
Sbjct: 350 EKRRILSFVGGIDLCDGRYDTPFHSLFRTLDT-AHHDDFHQPNFPDGAITKGGPREPWHD 409
Query: 401 VHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSIL--LKLMPQLESNTSTQKDWHVQVF 460
+H+ + G AWD+L NFEQRW KQ +LV L + + P + + W+VQ+F
Sbjct: 410 IHSRLEGPIAWDVLFNFEQRWRKQGGKDVLVNFRELDDIIIPPSPVMHLDDSETWNVQLF 469
Query: 461 RSIDHLSASQV--------------FRNLTVERTIHEAYVEAIRRAERFIYIENQYFIGG 520
RSID +A + ++R+I +AY+ AIRRA+ FIYIENQYF+G
Sbjct: 470 RSIDEGAAFGFPETPEDAAKAGLVSGXDNIIDRSIQDAYIHAIRRAKNFIYIENQYFLGS 529
Query: 521 CHLWDKD----QHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPESESVEDM 580
+ W D + G ++IP E+ALK+ +KI+A ERF VY+V+PMWPEG PES SV+ +
Sbjct: 530 SYDWQSDDIKVEDIGALHVIPKELALKIVSKIEAGERFTVYVVVPMWPEGIPESASVQAI 589
Query: 581 LHWTRQTMKMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEETKWDFVPPHSPQHATQY 640
L W R+TM+MMY+ I +A+ G + PR+YL FFC+ NRE + + P +P+ + Y
Sbjct: 590 LDWQRRTMEMMYKHIVQALNAKGIEEDPRNYLTFFCIGNREVKKSGAYEPSETPEPDSDY 649
Query: 641 WNAQQHRRFMVYVHSKVMIVDDSYILIGSANVNQRSMDGERDTEIAIGCYQLENDGEELP 700
AQ+ RRFM+YVHSK+MIVDD YI++GSAN+NQRSMDG RD+EIA+G YQ + P
Sbjct: 650 IRAQEARRFMIYVHSKMMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPHHLATREP 709
Query: 701 NGRDISTFRLSLWYEHTQRFEDLFLQPENLQCVQRVRSIADESWEIYSGDEI-ADMKGVH 760
I FR++LWYEH ++ FL PE+ +CV +V +AD+ W++YS + + D+ G H
Sbjct: 710 ARGQIHGFRMALWYEHLGMLDETFLHPESEECVSKVNRMADKYWDLYSSESLERDLPG-H 769
Query: 761 LVTYPVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMLLPPIFTT 762
L+ YP+ V +G + +L HFPDTK + G +S LPPI TT
Sbjct: 770 LLRYPIGVASEGDVTEL-PGAEHFPDTKARVLGTKSDYLPPILTT 808
BLAST of Spg023566 vs. ExPASy Swiss-Prot
Match:
Q70EW5 (Phospholipase D alpha 1 OS=Cynara cardunculus OX=4265 GN=PLD1 PE=1 SV=2)
HSP 1 Score: 601.3 bits (1549), Expect = 1.5e-170
Identity = 321/808 (39.73%), Postives = 475/808 (58.79%), Query Frame = 0
Query: 11 HGTLEVTVFHATAYTP---PSPLDCLFA----------GGKRSYVTIKIDNKEVAQT--- 70
HGTL VTV+ P+ L A G + Y TI ++ V +T
Sbjct: 7 HGTLHVTVYEVDKLREGGGPNVFGKLMANIQETVGFGEGTPKIYATIDLEKSRVGRTRMI 66
Query: 71 -SHEYDRVWNQTFRVLCAYPLTSTVAITMKTSR----SVLGRFQIQAQQILKEASFINGF 130
+ + W ++F + CA+ S + T+K ++LGR + +++L + ++ +
Sbjct: 67 ENEPQNPRWYESFHIYCAHH-ASNIIFTVKDDNPIGATLLGRAYMPVRELL-DGDEVDKW 126
Query: 131 FPLLMENGKPSPE-LRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLY 190
++ E+ P+P ++ L + + +W + + G+Y G+ F R C V+LY
Sbjct: 127 IEIMDEDNNPTPAGSKIHVKLQYFDVTQDRNWDRGIKTGKYPGVPYTFFAQRQGCRVSLY 186
Query: 191 QDAHHVPTFRPPFHGSNA----PRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQT 250
QDAH F P + P R WED++ AI +AKH +YI GWS ++ L+RD
Sbjct: 187 QDAHVPDNFIPKISLAGGKYYEPHRCWEDIFDAISDAKHFIYITGWSVYTQIPLIRDPNR 246
Query: 251 DIPYALGVKLGELLKQKAEEGVAVRLLIWDDETSLPIIKNAGIMKTHDEDARAYFLHSKV 310
P V LG+LLK+KA+EGV V +L+WDD TS+ + K G+M THDE+ +F + V
Sbjct: 247 QKPGG-DVLLGQLLKKKADEGVRVAMLVWDDRTSVNVFKEDGLMATHDEETENFFKDTDV 306
Query: 311 ICRLCPK--------LHPMS-PPIFSHHQKTIIVDAQ-THINARNREIMSFIGGLDLCDG 370
C LCP+ + + +F+HHQK ++VD + ++ R +MSF+GG+DLCDG
Sbjct: 307 HCILCPRDPDDGGSIIQDLKVSTMFTHHQKIVVVDHELPRGGSQKRRVMSFVGGIDLCDG 366
Query: 371 RYDTEQHSLFHTLNTESHCYDFYQTSISGAKLQKGGPREPWHDVHACVTGEAAWDILTNF 430
RYD+ H LF TL++ +H DF+Q + +GA + KGGPREPWHD+H+ V G AWD+L NF
Sbjct: 367 RYDSAFHPLFSTLDS-AHHDDFHQPNYAGASIAKGGPREPWHDIHSRVEGPIAWDVLFNF 426
Query: 431 EQRWTKQSDASLLVPTSIL--LKLMPQLESNTSTQKDWHVQVFRSID------------H 490
EQRW KQ ++LV L + + P + + Q+ W+VQ+FRSID
Sbjct: 427 EQRWRKQGGKNVLVDLKQLDDILIPPSPVTFPNDQETWNVQLFRSIDGGAAFGFPDTPEE 486
Query: 491 LSASQVF--RNLTVERTIHEAYVEAIRRAERFIYIENQYFIGGCHLWDKD----QHCGCT 550
S S + ++ ++R+I +AY+ AIRRA+ FIYIENQYF+G W D G
Sbjct: 487 ASKSGLVSGKDNIIDRSIQDAYINAIRRAKHFIYIENQYFLGSSFAWKSDDIDVDEVGAL 546
Query: 551 NLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPESESVEDMLHWTRQTMKMMYRLIGE 610
+LIP E++LK+ KI+ E+F VYIV+PMWPEG PE+ SV+ +L W R+TM+MMY+ I +
Sbjct: 547 HLIPKELSLKIVTKIQEGEKFIVYIVVPMWPEGIPENGSVQAILDWQRRTMEMMYKDIVD 606
Query: 611 AIQETGEKAHPRDYLNFFCLANREEETKWDFVPPHSPQHATQYWNAQQHRRFMVYVHSKV 670
A+Q+ G PR+YL FFCL NRE + ++ P +P+ + Y +AQ++RRFM+YVHSK+
Sbjct: 607 ALQDKGLDDDPREYLTFFCLGNREAKKSGEYEPTEAPEPDSGYLHAQENRRFMIYVHSKM 666
Query: 671 MIVDDSYILIGSANVNQRSMDGERDTEIAIGCYQLENDGEELPNGRDISTFRLSLWYEHT 730
MIVDD YI++GSAN+NQRSMDG RD+EIA+G YQ + + P + FR++LWYEH
Sbjct: 667 MIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPYHLATQTPARGHVHGFRMALWYEHL 726
Query: 731 QRFEDLFLQPENLQCVQRVRSIADESWEIYSGDEI-ADMKGVHLVTYPVKVKQDGSIEDL 762
+D F +PEN CV + +AD+ W++Y+ +++ D+ G HL+ YPV V + G I +L
Sbjct: 727 GMLDDSFERPENKDCVNKANEMADKCWDLYASEDLDRDLPG-HLLRYPVGVTRKGDITEL 786
BLAST of Spg023566 vs. ExPASy TrEMBL
Match:
A0A5D3BW81 (Phospholipase D OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold863G001380 PE=3 SV=1)
HSP 1 Score: 1511.5 bits (3912), Expect = 0.0e+00
Identity = 705/761 (92.64%), Postives = 739/761 (97.11%), Query Frame = 0
Query: 1 MAMEGKHKFFHGTLEVTVFHATAYTPPSPLDCLFAGGKRSYVTIKIDNKEVAQTSHEYDR 60
MAME K KFFHG LEVTVFHATAYTPPSPLDCLFAGGKRSYVTIKIDNKEVAQTSHE+DR
Sbjct: 1 MAMEVKQKFFHGMLEVTVFHATAYTPPSPLDCLFAGGKRSYVTIKIDNKEVAQTSHEHDR 60
Query: 61 VWNQTFRVLCAYPLTSTVAITMKTSRSVLGRFQIQAQQILKEASFINGFFPLLMENGKPS 120
VWNQTFRVLCAYPLTST+ IT+KT+RSVLG+F IQAQQILKEASFINGFFPLLMENGKPS
Sbjct: 61 VWNQTFRVLCAYPLTSTITITLKTARSVLGKFYIQAQQILKEASFINGFFPLLMENGKPS 120
Query: 121 PELRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHVPTFRPP 180
PEL+LRFMLWFKPAVYELSWKKML NGEYKGLRNATFPLRSNCHVTLYQDAHH+PTF+PP
Sbjct: 121 PELKLRFMLWFKPAVYELSWKKMLANGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPP 180
Query: 181 FHGSNAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ 240
FHGS+ PRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ
Sbjct: 181 FHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ 240
Query: 241 KAEEGVAVRLLIWDDETSLPIIKNAGIMKTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300
KAEEGVAVR+LIWDDETSLPIIKNAGIM THDEDARAYFLHSKVICRLCPKLHPMSPPIF
Sbjct: 241 KAEEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300
Query: 301 SHHQKTIIVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCYDFYQTS 360
SHHQK IIVDAQTHINA+NREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHC DFYQTS
Sbjct: 301 SHHQKIIIVDAQTHINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTS 360
Query: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420
ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL
Sbjct: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420
Query: 421 ESNTSTQKDWHVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 480
ESNT+ QKDW+VQVFRSIDHLSASQ+FRN+T+ERTIHEAYVEAIRRAERFIYIENQYFIG
Sbjct: 421 ESNTNPQKDWNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIG 480
Query: 481 GCHLWDKDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPESESVEDMLHW 540
GCH WD+D+HCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEG PESESVEDMLHW
Sbjct: 481 GCHFWDRDRHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPESESVEDMLHW 540
Query: 541 TRQTMKMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEETKWDFVPPHSPQHATQYWNA 600
TRQTM MMYRLIGEAIQETGEKAHPRDYLNFFCLANREEE KWDF+PPHSPQHATQYWNA
Sbjct: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQYWNA 600
Query: 601 QQHRRFMVYVHSKVMIVDDSYILIGSANVNQRSMDGERDTEIAIGCYQLENDGEELPNGR 660
QQHRRFM+YVHSKVMIVDD YILIGSANVNQRSMDGERDTEIAIGCYQ+ENDGEELPNGR
Sbjct: 601 QQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQIENDGEELPNGR 660
Query: 661 DISTFRLSLWYEHTQRFEDLFLQPENLQCVQRVRSIADESWEIYSGDEIADMKGVHLVTY 720
DIS FRLSLWYEHT+ FE++FL PE+L+CVQRVRSI D+SW+IYSG+E+ DMKGVHLVTY
Sbjct: 661 DISRFRLSLWYEHTRGFEEVFLNPESLKCVQRVRSIGDKSWKIYSGEEVEDMKGVHLVTY 720
Query: 721 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMLLPPIFTT 762
PVKVK+DG++EDLEENGGHFPDTKC IKGRRSM+LPPIFTT
Sbjct: 721 PVKVKEDGTMEDLEENGGHFPDTKCAIKGRRSMMLPPIFTT 761
BLAST of Spg023566 vs. ExPASy TrEMBL
Match:
A0A1S3BH90 (Phospholipase D OS=Cucumis melo OX=3656 GN=LOC103490014 PE=3 SV=1)
HSP 1 Score: 1511.5 bits (3912), Expect = 0.0e+00
Identity = 705/761 (92.64%), Postives = 739/761 (97.11%), Query Frame = 0
Query: 1 MAMEGKHKFFHGTLEVTVFHATAYTPPSPLDCLFAGGKRSYVTIKIDNKEVAQTSHEYDR 60
MAME K KFFHG LEVTVFHATAYTPPSPLDCLFAGGKRSYVTIKIDNKEVAQTSHE+DR
Sbjct: 1 MAMEVKQKFFHGMLEVTVFHATAYTPPSPLDCLFAGGKRSYVTIKIDNKEVAQTSHEHDR 60
Query: 61 VWNQTFRVLCAYPLTSTVAITMKTSRSVLGRFQIQAQQILKEASFINGFFPLLMENGKPS 120
VWNQTFRVLCAYPLTST+ IT+KT+RSVLG+F IQAQQILKEASFINGFFPLLMENGKPS
Sbjct: 61 VWNQTFRVLCAYPLTSTITITLKTARSVLGKFYIQAQQILKEASFINGFFPLLMENGKPS 120
Query: 121 PELRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHVPTFRPP 180
PEL+LRFMLWFKPAVYELSWKKML NGEYKGLRNATFPLRSNCHVTLYQDAHH+PTF+PP
Sbjct: 121 PELKLRFMLWFKPAVYELSWKKMLANGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPP 180
Query: 181 FHGSNAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ 240
FHGS+ PRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ
Sbjct: 181 FHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ 240
Query: 241 KAEEGVAVRLLIWDDETSLPIIKNAGIMKTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300
KAEEGVAVR+LIWDDETSLPIIKNAGIM THDEDARAYFLHSKVICRLCPKLHPMSPPIF
Sbjct: 241 KAEEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300
Query: 301 SHHQKTIIVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCYDFYQTS 360
SHHQK IIVDAQTHINA+NREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHC DFYQTS
Sbjct: 301 SHHQKIIIVDAQTHINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTS 360
Query: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420
ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL
Sbjct: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420
Query: 421 ESNTSTQKDWHVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 480
ESNT+ QKDW+VQVFRSIDHLSASQ+FRN+T+ERTIHEAYVEAIRRAERFIYIENQYFIG
Sbjct: 421 ESNTNPQKDWNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIG 480
Query: 481 GCHLWDKDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPESESVEDMLHW 540
GCH WD+D+HCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEG PESESVEDMLHW
Sbjct: 481 GCHFWDRDRHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPESESVEDMLHW 540
Query: 541 TRQTMKMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEETKWDFVPPHSPQHATQYWNA 600
TRQTM MMYRLIGEAIQETGEKAHPRDYLNFFCLANREEE KWDF+PPHSPQHATQYWNA
Sbjct: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQYWNA 600
Query: 601 QQHRRFMVYVHSKVMIVDDSYILIGSANVNQRSMDGERDTEIAIGCYQLENDGEELPNGR 660
QQHRRFM+YVHSKVMIVDD YILIGSANVNQRSMDGERDTEIAIGCYQ+ENDGEELPNGR
Sbjct: 601 QQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQIENDGEELPNGR 660
Query: 661 DISTFRLSLWYEHTQRFEDLFLQPENLQCVQRVRSIADESWEIYSGDEIADMKGVHLVTY 720
DIS FRLSLWYEHT+ FE++FL PE+L+CVQRVRSI D+SW+IYSG+E+ DMKGVHLVTY
Sbjct: 661 DISRFRLSLWYEHTRGFEEVFLNPESLKCVQRVRSIGDKSWKIYSGEEVEDMKGVHLVTY 720
Query: 721 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMLLPPIFTT 762
PVKVK+DG++EDLEENGGHFPDTKC IKGRRSM+LPPIFTT
Sbjct: 721 PVKVKEDGTMEDLEENGGHFPDTKCAIKGRRSMMLPPIFTT 761
BLAST of Spg023566 vs. ExPASy TrEMBL
Match:
A0A6J1G9V5 (Phospholipase D OS=Cucurbita moschata OX=3662 GN=LOC111452112 PE=3 SV=1)
HSP 1 Score: 1507.3 bits (3901), Expect = 0.0e+00
Identity = 706/761 (92.77%), Postives = 740/761 (97.24%), Query Frame = 0
Query: 1 MAMEGKHKFFHGTLEVTVFHATAYTPPSPLDCLFAGGKRSYVTIKIDNKEVAQTSHEYDR 60
M ++GKHKFFHGTLEVTVFHAT YTP SPLDC+F+GGK+SYVTIKI+N EVA+T+HEYDR
Sbjct: 62 MTVQGKHKFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR 121
Query: 61 VWNQTFRVLCAYPLTSTVAITMKTSRSVLGRFQIQAQQILKEASFINGFFPLLMENGKPS 120
VWNQTFRVLCA+PLTST+ ITM+T+RSVLGRFQIQAQQILKE+SFINGFFPLLMENGKPS
Sbjct: 122 VWNQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS 181
Query: 121 PELRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHVPTFRPP 180
PELRLRFMLWF+PAVYELSWKK+L NGEYKGLRNATFPLRSNCHVTLYQDAHHV TF+PP
Sbjct: 182 PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNATFPLRSNCHVTLYQDAHHVSTFQPP 241
Query: 181 FHGSNAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ 240
FHGS+APRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRD QT IPYALGVKLGELLKQ
Sbjct: 242 FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTHIPYALGVKLGELLKQ 301
Query: 241 KAEEGVAVRLLIWDDETSLPIIKNAGIMKTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300
KAEEGVAVRL+IWDDETSLPIIKNAGIMKTHDEDARAYF +SKV+CRLCPKLHPMSPPIF
Sbjct: 302 KAEEGVAVRLMIWDDETSLPIIKNAGIMKTHDEDARAYFSNSKVVCRLCPKLHPMSPPIF 361
Query: 301 SHHQKTIIVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCYDFYQTS 360
SHHQKTI VDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHC DFYQTS
Sbjct: 362 SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS 421
Query: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420
ISGAKLQKGGPREPWHDVHA VTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMP L
Sbjct: 422 ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL 481
Query: 421 ESNTSTQKDWHVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 480
ESNT+ QKDW+VQVFRSI+HLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG
Sbjct: 482 ESNTNPQKDWNVQVFRSINHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 541
Query: 481 GCHLWDKDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPESESVEDMLHW 540
GCHLWDKDQHCGCTNLIPIEIALKVANKIKARE+FAVY+VIPMWPEGPPESESVEDMLHW
Sbjct: 542 GCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHW 601
Query: 541 TRQTMKMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEETKWDFVPPHSPQHATQYWNA 600
TRQTM MMYRLIGEAIQETGEKAHPRDYLNFFCLANREEE KWDFVPPHSPQHAT+YW+A
Sbjct: 602 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA 661
Query: 601 QQHRRFMVYVHSKVMIVDDSYILIGSANVNQRSMDGERDTEIAIGCYQLENDGEELPNGR 660
Q HRRFMVYVHSKVMIVDD YILIGSANVNQRSMDGERDTEIAIGCYQLENDG+ELPNGR
Sbjct: 662 QHHRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR 721
Query: 661 DISTFRLSLWYEHTQRFEDLFLQPENLQCVQRVRSIADESWEIYSGDEIADMKGVHLVTY 720
DISTFRLSLWYEHTQRFE++FL PENLQCV+RVRSIADESW+IYSG+E+ADMKGVHLVTY
Sbjct: 722 DISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTY 781
Query: 721 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMLLPPIFTT 762
PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSM LPPIFTT
Sbjct: 782 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 822
BLAST of Spg023566 vs. ExPASy TrEMBL
Match:
A0A6J1KAT1 (Phospholipase D OS=Cucurbita maxima OX=3661 GN=LOC111492613 PE=3 SV=1)
HSP 1 Score: 1500.3 bits (3883), Expect = 0.0e+00
Identity = 701/761 (92.12%), Postives = 738/761 (96.98%), Query Frame = 0
Query: 1 MAMEGKHKFFHGTLEVTVFHATAYTPPSPLDCLFAGGKRSYVTIKIDNKEVAQTSHEYDR 60
M ++G+H FFHGTLEVTVFHAT YTP SPLDC+F+GGK+SYVTIKI+N EVA+T+HEYDR
Sbjct: 1 MTVQGEHTFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR 60
Query: 61 VWNQTFRVLCAYPLTSTVAITMKTSRSVLGRFQIQAQQILKEASFINGFFPLLMENGKPS 120
VWNQTFRVLCA+PLTST+ ITM+T+RSVLGRFQIQAQQILKE+SFINGFFPLLMENGKPS
Sbjct: 61 VWNQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS 120
Query: 121 PELRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHVPTFRPP 180
PELRLRFMLWF+PAVYELSWKK+L NGEYKGLRN TFPLRSNCHVTLYQDAHHV TF+PP
Sbjct: 121 PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNVTFPLRSNCHVTLYQDAHHVSTFQPP 180
Query: 181 FHGSNAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ 240
FHGS+APRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRD QTDIPYALGVKLGELLKQ
Sbjct: 181 FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTDIPYALGVKLGELLKQ 240
Query: 241 KAEEGVAVRLLIWDDETSLPIIKNAGIMKTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300
KAEEGVAVRL+IWDDETSLP+IKNAGIMKTHDE+ARAYF +SKV+CRLCPKLHPMSPPIF
Sbjct: 241 KAEEGVAVRLMIWDDETSLPVIKNAGIMKTHDENARAYFSNSKVVCRLCPKLHPMSPPIF 300
Query: 301 SHHQKTIIVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCYDFYQTS 360
SHHQKTI VDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHC DFYQTS
Sbjct: 301 SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS 360
Query: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420
ISGAKLQKGGPREPWHDVHA VTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMP L
Sbjct: 361 ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL 420
Query: 421 ESNTSTQKDWHVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 480
ESNT+ QKDW+VQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG
Sbjct: 421 ESNTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 480
Query: 481 GCHLWDKDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPESESVEDMLHW 540
GCHLWDKDQHCGCTNLIPIEIALKVANKIKARE+FAVY+VIPMWPEGPPESESVEDMLHW
Sbjct: 481 GCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHW 540
Query: 541 TRQTMKMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEETKWDFVPPHSPQHATQYWNA 600
TRQTM MMYRLIGEAIQETGEKAHPRDYLNFFCLANREEE KWDFVPPHSPQHAT+YW+A
Sbjct: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA 600
Query: 601 QQHRRFMVYVHSKVMIVDDSYILIGSANVNQRSMDGERDTEIAIGCYQLENDGEELPNGR 660
Q RRFMVYVHSKVMIVDD Y+LIGSANVNQRSMDGERDTEIAIGCYQLENDG+ELPNGR
Sbjct: 601 QHRRRFMVYVHSKVMIVDDLYLLIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR 660
Query: 661 DISTFRLSLWYEHTQRFEDLFLQPENLQCVQRVRSIADESWEIYSGDEIADMKGVHLVTY 720
DISTFRLSLWYEHTQRFE++FL PENLQCV+RVRSIADESW+IYSG+E+ADMKGVHLVTY
Sbjct: 661 DISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTY 720
Query: 721 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMLLPPIFTT 762
PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSM LPPIFTT
Sbjct: 721 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 761
BLAST of Spg023566 vs. ExPASy TrEMBL
Match:
A0A6J1D3G4 (Phospholipase D OS=Momordica charantia OX=3673 GN=LOC111016958 PE=3 SV=1)
HSP 1 Score: 1487.2 bits (3849), Expect = 0.0e+00
Identity = 695/761 (91.33%), Postives = 727/761 (95.53%), Query Frame = 0
Query: 1 MAMEGKHKFFHGTLEVTVFHATAYTPPSPLDCLFAGGKRSYVTIKIDNKEVAQTSHEYDR 60
M MEGK FFHGTLEVTVFHATAYTPPSPLDCLF+GG+R+YVT+KIDNKEVAQTSHEYDR
Sbjct: 1 MEMEGKQMFFHGTLEVTVFHATAYTPPSPLDCLFSGGRRTYVTVKIDNKEVAQTSHEYDR 60
Query: 61 VWNQTFRVLCAYPLTSTVAITMKTSRSVLGRFQIQAQQILKEASFINGFFPLLMENGKPS 120
VWNQTF++LCAYP TSTV ITMKTSRSVLG+F IQAQQILKEASF+NGFFPL MENGKPS
Sbjct: 61 VWNQTFQILCAYPSTSTVTITMKTSRSVLGKFHIQAQQILKEASFMNGFFPLTMENGKPS 120
Query: 121 PELRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHVPTFRPP 180
PELRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHH+PTF+PP
Sbjct: 121 PELRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPP 180
Query: 181 FHGSNAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ 240
FH S++PRRLWEDVYKAIDNAKHL+YIAGWSFNPKM+LVRDSQTDIPYALGVKLGELLKQ
Sbjct: 181 FHSSSSPRRLWEDVYKAIDNAKHLIYIAGWSFNPKMLLVRDSQTDIPYALGVKLGELLKQ 240
Query: 241 KAEEGVAVRLLIWDDETSLPIIKNAGIMKTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300
KAEEGVAVR++IWDD TSLPIIKNAGIMKT DEDA AYF HSKVICRLCPKLHPM+PPIF
Sbjct: 241 KAEEGVAVRIMIWDDATSLPIIKNAGIMKTRDEDAWAYFSHSKVICRLCPKLHPMAPPIF 300
Query: 301 SHHQKTIIVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCYDFYQTS 360
SHHQKTI VDAQ HINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLN ESHC DFYQTS
Sbjct: 301 SHHQKTITVDAQAHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNMESHCGDFYQTS 360
Query: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420
ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILL LMPQL
Sbjct: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLNLMPQL 420
Query: 421 ESNTSTQKDWHVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 480
E+NT+ Q DW+VQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG
Sbjct: 421 ETNTNPQNDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 480
Query: 481 GCHLWDKDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPESESVEDMLHW 540
GCHLWDKDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPESESV +MLHW
Sbjct: 481 GCHLWDKDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPESESVAEMLHW 540
Query: 541 TRQTMKMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEETKWDFVPPHSPQHATQYWNA 600
TRQTMKMMY+LIGEAIQETGEKAHPRDYLNFFCLANREEE +WDFVPPHSP+ ATQYWNA
Sbjct: 541 TRQTMKMMYKLIGEAIQETGEKAHPRDYLNFFCLANREEEGEWDFVPPHSPERATQYWNA 600
Query: 601 QQHRRFMVYVHSKVMIVDDSYILIGSANVNQRSMDGERDTEIAIGCYQLENDGEELPNGR 660
QQHRRFMVYVHSKVMIVDD YILIGSANVNQRSMDG RDTEIAIGC+Q E D EE+PNGR
Sbjct: 601 QQHRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGGRDTEIAIGCFQQEKDAEEMPNGR 660
Query: 661 DISTFRLSLWYEHTQRFEDLFLQPENLQCVQRVRSIADESWEIYSGDEIADMKGVHLVTY 720
DISTFRLSLWYEHT+RFE+LFL PE L CVQRVRSIADESW IYSG+E ADM+GVHLV Y
Sbjct: 661 DISTFRLSLWYEHTRRFEELFLNPETLDCVQRVRSIADESWRIYSGEEAADMEGVHLVAY 720
Query: 721 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMLLPPIFTT 762
PVKV Q+GS+EDLEENGGHFPDTKCPIKGRRSMLLPPIFTT
Sbjct: 721 PVKVNQEGSVEDLEENGGHFPDTKCPIKGRRSMLLPPIFTT 761
BLAST of Spg023566 vs. TAIR 10
Match:
AT1G55180.1 (phospholipase D alpha 4 )
HSP 1 Score: 980.3 bits (2533), Expect = 8.6e-286
Identity = 463/776 (59.66%), Postives = 600/776 (77.32%), Query Frame = 0
Query: 1 MAMEGKHKFFHGTLEVTVFHATAYTPPSPLDCLFAGGKRSYVTIKIDNKEVAQTSHEYDR 60
M +E + K+FHGTLE+T+F AT ++PP P +C+ K +YVTIKI+ K+VA+TS EYDR
Sbjct: 1 MELEEQKKYFHGTLEITIFDATPFSPPFPFNCICTKPKAAYVTIKINKKKVAKTSSEYDR 60
Query: 61 VWNQTFRVLCAYPLT-STVAITMKTSRSVLGRFQIQAQQIL-KEASFINGFFPLLMENGK 120
+WNQTF++LCA+P+T +T+ IT+KT SVLGRF+I A+QIL ++ INGFFPL+ +NG
Sbjct: 61 IWNQTFQILCAHPVTDTTITITLKTRCSVLGRFRISAEQILTSNSAVINGFFPLIADNGS 120
Query: 121 PSPELRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHVPTFR 180
L+L+ ++WF+PA E W + L ++G+RNA+FP RSNC V LYQDAHH TF
Sbjct: 121 TKRNLKLKCLMWFRPAYLEPGWCRALEEASFQGIRNASFPQRSNCRVVLYQDAHHKATFD 180
Query: 181 P-----PFHGSNAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVK 240
P PF+ R LWEDVYKAI++A+HLVYIAGW+ NP +VLVRD++T+IP+A+GV
Sbjct: 181 PRVDDVPFNA----RNLWEDVYKAIESARHLVYIAGWALNPNLVLVRDNETEIPHAVGVT 240
Query: 241 LGELLKQKAEEGVAVRLLIWDDETSLPIIKNAGIMKTHDEDARAYFLHSKVICRLCPKLH 300
+GELLK+K+EEGVAVR+++W+DETSLP+IKN G+M+T+ E A AYF ++ V+CRLCP+LH
Sbjct: 241 VGELLKRKSEEGVAVRVMLWNDETSLPMIKNKGVMRTNVERALAYFRNTNVVCRLCPRLH 300
Query: 301 PMSPPIFSHHQKTIIVDAQ-THINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESH 360
P F+HHQKTI +D + T+ + + REIMSF+GG DLCDGRYDTE+HSLF TL TE+
Sbjct: 301 KKLPTAFAHHQKTITLDTRVTNSSTKEREIMSFLGGFDLCDGRYDTEEHSLFRTLGTEA- 360
Query: 361 CYDFYQTSISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSI 420
DFYQTS++GAKL +GGPREPWHD H V G AAWD+L NFEQRWTKQ + S+LV TS
Sbjct: 361 --DFYQTSVAGAKLSRGGPREPWHDCHVSVVGGAAWDVLKNFEQRWTKQCNPSVLVNTSG 420
Query: 421 LLKLMPQLESNTSTQKDWHVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIY 480
+ L+ + W+VQV RSIDH+SA+++ R L VE+++H+ YV AIR+AERFIY
Sbjct: 421 IRNLVNLTGPTEENNRKWNVQVLRSIDHISATEMPRGLPVEKSVHDGYVAAIRKAERFIY 480
Query: 481 IENQYFIGGCHLWD--KDQHC-GCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPP 540
IENQYF+G C W+ D+ C GCTNLIP+EIALK+A KI+ARERFAVYIVIPMWPEGPP
Sbjct: 481 IENQYFMGSCDHWESKNDKICSGCTNLIPVEIALKIAAKIRARERFAVYIVIPMWPEGPP 540
Query: 541 ESESVEDMLHWTRQTMKMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEETKWDFVPPH 600
ESE+VE++LHWTR+TM MMY++IGEAI E G+K+HPRDYLNFFCLANREE+ +F
Sbjct: 541 ESETVEEILHWTRETMSMMYQIIGEAIWEVGDKSHPRDYLNFFCLANREEKRDGEFEAVS 600
Query: 601 SPQHATQYWNAQQHRRFMVYVHSKVMIVDDSYILIGSANVNQRSMDGERDTEIAIGCYQL 660
SP T YWNAQ++RRFMVYVHSK+MIVDD+YILIGSAN+NQRSMDG RDTEIAIGCYQ
Sbjct: 601 SPHQKTHYWNAQRNRRFMVYVHSKLMIVDDTYILIGSANINQRSMDGCRDTEIAIGCYQT 660
Query: 661 ENDGEELPNGRDISTFRLSLWYEHTQ---RFEDL-FLQPENLQCVQRVRSIADESWEIYS 720
+ N +I +RLSLWYEHT +DL +PE+L+CV+ +R+I ++ WEIYS
Sbjct: 661 NTN-----NTNEIQAYRLSLWYEHTGGKITADDLSSSEPESLECVRGLRTIGEQMWEIYS 720
Query: 721 GDEIADMKGVHLVTYPVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMLLPPIFTT 762
GD++ DM G+HLV YP+ V DG++E++ + G FPDTK +KG+RS + PP+ TT
Sbjct: 721 GDKVVDMLGIHLVAYPISVTGDGAVEEVGD--GCFPDTKTLVKGKRSKMFPPVLTT 762
BLAST of Spg023566 vs. TAIR 10
Match:
AT5G25370.1 (phospholipase D alpha 3 )
HSP 1 Score: 599.4 bits (1544), Expect = 4.1e-171
Identity = 335/776 (43.17%), Postives = 461/776 (59.41%), Query Frame = 0
Query: 32 CLFAGGKRSYVTIKIDNKEVAQTSHEYDRVWNQTFRVLCAYPLTSTVAITMK----TSRS 91
C G Y TI +D VA+T W Q+F V A+ + S + T+K S S
Sbjct: 53 CTSLFGGHLYATIDLDRSRVARTMMRRHPKWLQSFHVYTAHSI-SKIIFTVKEDEPVSAS 112
Query: 92 VLGRFQIQAQQILKEASFINGFFPLLMENGKP-SPELRLRFMLWFKPAVYELSWKKMLGN 151
++GR + +++ I+ + +L EN +P +L + F +++W K +
Sbjct: 113 LIGRAYLPVTEVI-TGQPIDRWLDILDENRRPIQGGSKLHVRVKFTHVTQDVNWNKGIIL 172
Query: 152 GEYKGLRNATFPLRSNCHVTLYQDAHHVPTFRP-PFHGSNA---PRRLWEDVYKAIDNAK 211
+ G+ NA F R C VTLYQDAH + + G R WE+++ AI AK
Sbjct: 173 PSFNGVPNAYFNQREGCKVTLYQDAHVLNEYPDVTLTGGQVIYKHHRCWEEIFDAIWEAK 232
Query: 212 HLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQKAEEGVAVRLLIWDDETSLPII 271
HL+YIAGWS N + LVRD + P +KLGELLK+KAEE V V +L+WDD TS +
Sbjct: 233 HLIYIAGWSVNTDVTLVRDPKRTRPGG-DLKLGELLKKKAEENVTVLMLVWDDRTSHEVF 292
Query: 272 KNAGIMKTHDEDARAYFLHSKVICRLCPKLHPMSPPI---------FSHHQKTIIVDAQT 331
K G+M THD++ YF ++KV C LCP+ I F+HHQKTI+VD++
Sbjct: 293 KRDGLMMTHDQETYDYFKNTKVRCVLCPRNPDNGDSIVQGFEVATMFTHHQKTIVVDSEV 352
Query: 332 HINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCYDFYQTSISGAKLQKGGPRE 391
+ R I+SF+GG+DLCDGRYDT +H LF TLN+ H DF+Q + GA ++KGGPRE
Sbjct: 353 DGSLTKRRIVSFLGGIDLCDGRYDTVEHPLFGTLNS-VHANDFHQPNFDGASIKKGGPRE 412
Query: 392 PWHDVHACVTGEAAWDILTNFEQRWTKQ-SDASLLVPTSILLKL-MPQLE-SNTSTQKDW 451
PWHD+H + G AAWD+L NFEQRW KQ S L+ + L ++ +P L ++ W
Sbjct: 413 PWHDIHCKLDGPAAWDVLYNFEQRWMKQGSGRRYLISMAQLAEITVPPLPIVQPDNEEGW 472
Query: 452 HVQVFRSIDHLSASQVF---------------RNLTVERTIHEAYVEAIRRAERFIYIEN 511
VQVFRSID A + F ++ +ER+I +AYV AIRRA+ FIYIEN
Sbjct: 473 TVQVFRSIDD-GAVEGFPEDPREAASIGLISGKDNVIERSIQDAYVNAIRRAKNFIYIEN 532
Query: 512 QYFIGGCHLWDKD----QHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPES 571
QYF+G W+ LIP EI+LK+ +KI+A ERF+VYIVIP+WPEG P S
Sbjct: 533 QYFLGSSFGWNSRDINLNEINALQLIPKEISLKIVSKIEAGERFSVYIVIPLWPEGKPGS 592
Query: 572 ESVEDMLHWTRQTMKMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEETKWDFVPPHSP 631
SV+ +L W R+TM+MMY I A+++ G A+PRDYL FFCL NRE+ +++PP P
Sbjct: 593 ASVQAILDWQRRTMEMMYTDIIIALRKKGLDANPRDYLTFFCLGNREKGKVGEYLPPEKP 652
Query: 632 QHATQYWNAQQHRRFMVYVHSKVMIVDDSYILIGSANVNQRSMDGERDTEIAIGCYQ--- 691
+ + Y AQ+ RRFM+YVHSK+MIVDD YI+IGSAN+NQRSMDG RDTEIA+G YQ
Sbjct: 653 EANSDYARAQESRRFMIYVHSKMMIVDDEYIIIGSANINQRSMDGGRDTEIAMGAYQPSH 712
Query: 692 LENDGEELPNGRDISTFRLSLWYEHTQRFEDLFLQPENLQCVQRVRSIADESWEIYSGDE 751
L + P G+ I +FR+SLW EH + + F PE+ +C++ V + ADE W +YS E
Sbjct: 713 LLSTNNMRPVGQ-IFSFRISLWLEHLRVTTNAFQCPESEECIRMVNATADELWGLYSAQE 772
Query: 752 IA---DMKGVHLVTYPVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMLLPPIFTT 762
D+ G HL++YP+ + +G + +L FPDT + G +S LPPI T+
Sbjct: 773 YPRNDDLPG-HLLSYPISIGSNGEVTNL-AGTEFFPDTNAKVVGEKSNYLPPILTS 820
BLAST of Spg023566 vs. TAIR 10
Match:
AT3G15730.1 (phospholipase D alpha 1 )
HSP 1 Score: 593.2 bits (1528), Expect = 3.0e-169
Identity = 328/820 (40.00%), Postives = 469/820 (57.20%), Query Frame = 0
Query: 11 HGTLEVTVFHATAYTPPSPLDCLFAGGKRS-----------------------YVTIKID 70
HGTL HAT Y +D L GG R Y TI +
Sbjct: 7 HGTL-----HATIY----EVDALHGGGVRQGFLGKILANVEETIGVGKGETQLYATIDLQ 66
Query: 71 NKEVAQT----SHEYDRVWNQTFRVLCAYPLTSTVAITMKTSR----SVLGRFQIQAQQI 130
V +T + + W ++F + CA+ L S + T+K +++GR I Q+
Sbjct: 67 KARVGRTRKIKNEPKNPKWYESFHIYCAH-LASDIIFTVKDDNPIGATLIGRAYIPVDQV 126
Query: 131 LKEASFINGFFPLLMENGKP-SPELRLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFP 190
+ ++ + +L + P ++ L + + +W + + ++ G+ F
Sbjct: 127 I-NGEEVDQWVEILDNDRNPIQGGSKIHVKLQYFHVEEDRNWNMGIKSAKFPGVPYTFFS 186
Query: 191 LRSNCHVTLYQDAHHVPTFRP--PFHGSN--APRRLWEDVYKAIDNAKHLVYIAGWSFNP 250
R C V+LYQDAH F P P G P+R WED++ AI NAKHL+YI GWS
Sbjct: 187 QRQGCKVSLYQDAHIPDNFVPRIPLAGGKNYEPQRCWEDIFDAISNAKHLIYITGWSVYA 246
Query: 251 KMVLVRDSQTDIPYALGVKLGELLKQKAEEGVAVRLLIWDDETSLPIIKNAGIMKTHDED 310
++ LVRDS+ P V +GELLK+KA EGV V LL+WDD TS+ ++K G+M THDE+
Sbjct: 247 EIALVRDSRRPKPGG-DVTIGELLKKKASEGVRVLLLVWDDRTSVDVLKKDGLMATHDEE 306
Query: 311 ARAYFLHSKVICRLCPKLHP----------MSPPIFSHHQKTIIVDAQ--THINARNREI 370
+F S V C LCP+ +P +F+HHQK ++VD++ + + R I
Sbjct: 307 TENFFRGSDVHCILCPR-NPDDGGSIVQSLQISTMFTHHQKIVVVDSEMPSRGGSEMRRI 366
Query: 371 MSFIGGLDLCDGRYDTEQHSLFHTLNTESHCYDFYQTSISGAKLQKGGPREPWHDVHACV 430
+SF+GG+DLCDGRYDT HSLF TL+T H DF+Q + +GA + KGGPREPWHD+H+ +
Sbjct: 367 VSFVGGIDLCDGRYDTPFHSLFRTLDTVHH-DDFHQPNFTGAAITKGGPREPWHDIHSRL 426
Query: 431 TGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQLESNTSTQKD---WHVQVFRSID 490
G AWD++ NFEQRW+KQ +LV L ++ S Q+D W+VQ+FRSID
Sbjct: 427 EGPIAWDVMYNFEQRWSKQGGKDILVKLRDLSDII-ITPSPVMFQEDHDVWNVQLFRSID 486
Query: 491 HLSASQV--------------FRNLTVERTIHEAYVEAIRRAERFIYIENQYFIGGCHLW 550
+A+ ++ ++R+I +AY+ AIRRA+ FIY+ENQYF+G W
Sbjct: 487 GGAAAGFPESPEAAAEAGLVSGKDNIIDRSIQDAYIHAIRRAKDFIYVENQYFLGSSFAW 546
Query: 551 DKD----QHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPESESVEDMLHWT 610
D + +LIP E++LK+ +KI+ E+F VY+V+PMWPEG PES SV+ +L W
Sbjct: 547 AADGITPEDINALHLIPKELSLKIVSKIEKGEKFRVYVVVPMWPEGLPESGSVQAILDWQ 606
Query: 611 RQTMKMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEETKWDFVPPHSPQHATQYWNAQ 670
R+TM+MMY+ + +A++ G + PR+YL FFCL NRE + ++ P P T Y AQ
Sbjct: 607 RRTMEMMYKDVIQALRAQGLEEDPRNYLTFFCLGNREVKKDGEYEPAEKPDPDTDYMRAQ 666
Query: 671 QHRRFMVYVHSKVMIVDDSYILIGSANVNQRSMDGERDTEIAIGCYQLENDGEELPNGRD 730
+ RRFM+YVH+K+MIVDD YI+IGSAN+NQRSMDG RD+EIA+G YQ + P
Sbjct: 667 EARRFMIYVHTKMMIVDDEYIIIGSANINQRSMDGARDSEIAMGGYQPHHLSHRQPARGQ 726
Query: 731 ISTFRLSLWYEHTQRFEDLFLQPENLQCVQRVRSIADESWEIYSGDEIADMKGVHLVTYP 762
I FR+SLWYEH ++ FL P +L+C+++V I+D+ W+ YS + + HL+ YP
Sbjct: 727 IHGFRMSLWYEHLGMLDETFLDPSSLECIEKVNRISDKYWDFYSSESLEHDLPGHLLRYP 786
BLAST of Spg023566 vs. TAIR 10
Match:
AT1G52570.1 (phospholipase D alpha 2 )
HSP 1 Score: 589.0 bits (1517), Expect = 5.6e-168
Identity = 319/772 (41.32%), Postives = 456/772 (59.07%), Query Frame = 0
Query: 36 GGKRSYVTIKIDNKEVAQ----TSHEYDRVWNQTFRVLCAYPLTSTVAITMKTSR----S 95
G + Y TI ++ V + T + W ++F + C + + V T+K + +
Sbjct: 46 GETQLYATIDLEKARVGRTRKITKEPKNPKWFESFHIYCGH-MAKHVIFTVKDANPIGAT 105
Query: 96 VLGRFQIQAQQILKEASFINGFFPLLMENGKPSPELRLRFMLWFKPAVYELSWKKMLGNG 155
++GR I + IL L E + ++ L + + +W + + +
Sbjct: 106 LIGRGYIPVEDILHGEEVDRWVDILDNEKNPIAGGSKIHVKLQYFGVEKDKNWNRGIKSA 165
Query: 156 EYKGLRNATFPLRSNCHVTLYQDAHHVPTFRP--PFHGSN--APRRLWEDVYKAIDNAKH 215
++ G+ F R C V+LYQDAH F P P G P R WED++ AI NAKH
Sbjct: 166 KFPGVPYTFFSQRRGCKVSLYQDAHIPGNFVPKIPLAGGKNYEPHRCWEDIFDAITNAKH 225
Query: 216 LVYIAGWSFNPKMVLVRDSQTDIPYALG-VKLGELLKQKAEEGVAVRLLIWDDETSLPII 275
L+YI GWS ++ LVRDS+ P G V +GELLK+KA EGV V LL+WDD TS+ ++
Sbjct: 226 LIYITGWSVYTEISLVRDSRR--PKQGGDVTVGELLKKKASEGVKVILLVWDDRTSVDLL 285
Query: 276 KNAGIMKTHDEDARAYFLHSKVICRLCPK---------LHPMSPPIFSHHQKTIIVDAQT 335
K G+M THDE+ +F + V C LCP+ + +F+HHQK ++VD++
Sbjct: 286 KKDGLMATHDEETENFFRGTDVNCILCPRNPDDGGSIVQNLQISTMFTHHQKIVVVDSEM 345
Query: 336 HI-NARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCYDFYQTSISGAKLQKGGPR 395
+R+R I+SF+GGLDLCDGRYDT HSLF TL+T +H DF+Q + +GA + KGGPR
Sbjct: 346 PSGGSRSRRIVSFVGGLDLCDGRYDTPFHSLFRTLDT-AHHDDFHQPNFTGAAITKGGPR 405
Query: 396 EPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPT----SILLKLMPQLESNTSTQK 455
EPWHD+H + G AWD+L NFEQRW++Q +LV I++ P L S
Sbjct: 406 EPWHDIHCRLEGPIAWDVLYNFEQRWSRQGGKDILVKMRELGDIIIPPSPVLFS--EDHD 465
Query: 456 DWHVQVFRSIDHLSASQV--------------FRNLTVERTIHEAYVEAIRRAERFIYIE 515
W+VQ+FRSID +A+ ++ ++R+I +AY+ AIRRA+ FIYIE
Sbjct: 466 VWNVQLFRSIDGGAAAGFPDSPEAAAEAGLVSGKDNIIDRSIQDAYIHAIRRAKDFIYIE 525
Query: 516 NQYFIGGCHLWDKD----QHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGPPE 575
NQYF+G W D + +LIP E++LK+ +KIKA E+F VY+V+PMWPEG PE
Sbjct: 526 NQYFLGSSFAWSADGIKPEEINALHLIPKELSLKIVSKIKAGEKFKVYVVVPMWPEGIPE 585
Query: 576 SESVEDMLHWTRQTMKMMYRLIGEAIQETG-EKAHPRDYLNFFCLANREEETKWDFVPPH 635
S SV+ +L W ++TM+MMY+ + +A++E G E PRDYL FFCL NRE + ++ P
Sbjct: 586 SGSVQAILDWQKRTMEMMYKDVIKALRENGLEGEDPRDYLTFFCLGNREVKKDGEYEPSE 645
Query: 636 SPQHATQYWNAQQHRRFMVYVHSKVMIVDDSYILIGSANVNQRSMDGERDTEIAIGCYQL 695
P+ T Y AQ+ RRFM+YVH+K+MIVDD YI+IGSAN+NQRSMDG RD+EIA+G YQ
Sbjct: 646 KPEPDTDYIRAQEARRFMIYVHTKMMIVDDEYIIIGSANINQRSMDGARDSEIAMGGYQP 705
Query: 696 ENDGEELPNGRDISTFRLSLWYEHTQRFEDLFLQPENLQCVQRVRSIADESWEIYSGDEI 755
+ P I FR+SLWYEH ++ FL P + +C+Q+V +AD+ W++YS + +
Sbjct: 706 YHLSTRQPARGQIHGFRMSLWYEHLGMLDETFLDPSSQECIQKVNRVADKYWDLYSSESL 765
Query: 756 ADMKGVHLVTYPVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMLLPPIFTT 762
HL+ YP+ + +G+I +L FPDTK I G +S +PPI TT
Sbjct: 766 EHDLPGHLLRYPIGIASEGNITEL-PGCEFFPDTKARILGVKSDYMPPILTT 810
BLAST of Spg023566 vs. TAIR 10
Match:
AT2G42010.1 (phospholipase D beta 1 )
HSP 1 Score: 540.8 bits (1392), Expect = 1.8e-153
Identity = 320/768 (41.67%), Postives = 442/768 (57.55%), Query Frame = 0
Query: 41 YVTIKIDNKEVAQT---SHEYDRVWNQTFRVLCAYPLTSTVAITMKTS----RSVLGRFQ 100
YV++ + + +T S+ + VW Q F V A+ + V +K S ++G
Sbjct: 319 YVSVSVAGAVIGRTYVMSNSENPVWMQHFYVPVAHH-AAEVHFVVKDSDVVGSQLIGLVT 378
Query: 101 IQAQQILKEASFINGFFPLLMENGKP-SPELRLRFMLWFKPAVYELSWKKMLGNG-EYKG 160
I +QI A I G +P+L NGKP P L + + P + +G G +Y+G
Sbjct: 379 IPVEQIYSGAK-IEGTYPILNSNGKPCKPGANLSLSIQYTPMDKLSVYHHGVGAGPDYQG 438
Query: 161 LRNATFPLRSNCHVTLYQDAH----HVPTFRPPFHGSNAPRRLWEDVYKAIDNAKHLVYI 220
+ FPLR V LYQDAH +P R S + W D++ AI A+ L+YI
Sbjct: 439 VPGTYFPLRKGGTVRLYQDAHVPEGMLPGIRLDNGMSYEHGKCWHDMFDAIRQARRLIYI 498
Query: 221 AGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQKAEEGVAVRLLIWDDETSLPII--KNA 280
GWS K+ L+RD + A LGELL+ K++EGV V LLIWDD TS I+ K
Sbjct: 499 TGWSVWHKVKLIRDK---LGPASECTLGELLRSKSQEGVRVLLLIWDDPTSRSILGYKTD 558
Query: 281 GIMKTHDEDARAYFLHSKVICRLCP----KLHPMSP-----PIFSHHQKTIIVDAQTHIN 340
G+M THDE+ R +F HS V LCP K H I++HHQK +IVDA
Sbjct: 559 GVMATHDEETRRFFKHSSVQVLLCPRNAGKRHSWVKQREVGTIYTHHQKNVIVDADA--G 618
Query: 341 ARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCYDFYQTSISGAKLQKGGPREPWH 400
R+I++F+GGLDLCDGRYDT QH LF TL T H DF+ + +G G PREPWH
Sbjct: 619 GNRRKIIAFVGGLDLCDGRYDTPQHPLFRTLQT-IHKDDFHNPTFTGN--LSGCPREPWH 678
Query: 401 DVHACVTGEAAWDILTNFEQRWTKQS------------DASLL----VPTSILLKLMPQL 460
D+H+ + G AA+D+LTNFE+RW K + D +LL +P + + P +
Sbjct: 679 DLHSKIDGPAAYDVLTNFEERWLKAAKPSGIKKFKTSYDDALLRIDRIPDILGVSDTPTV 738
Query: 461 ESNTSTQKDWHVQVFRSIDHLSAS--------------QVFRNLTVERTIHEAYVEAIRR 520
N + WHVQ+FRSID S +N+ ++ +IH AYV+AIR
Sbjct: 739 SEN--DPEAWHVQIFRSIDSNSVKGFPKDPKDATCKNLVCGKNVLIDMSIHTAYVKAIRA 798
Query: 521 AERFIYIENQYFIGGCHLWDKDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPE 580
A+ FIYIENQYFIG + W+ + G NLIP+EIALK+A KI+A ERFA YIVIPMWPE
Sbjct: 799 AQHFIYIENQYFIGSSYNWNAHKDIGANNLIPMEIALKIAEKIRANERFAAYIVIPMWPE 858
Query: 581 GPPESESVEDMLHWTRQTMKMMYRLIGEAIQETGEKA--HPRDYLNFFCLANREEETKWD 640
G P + + +L+W +T++MMY I +A+ ETG + P+DYLNFFCL NRE D
Sbjct: 859 GVPTGAATQRILYWQHKTIQMMYETIYKALVETGLEGAFSPQDYLNFFCLGNREMVDGID 918
Query: 641 FVPPHSPQHA-TQYWNAQQHRRFMVYVHSKVMIVDDSYILIGSANVNQRSMDGERDTEIA 700
SP +A T +++ RRFMVYVHSK M+VDD Y++IGSAN+NQRSM+G RDTEIA
Sbjct: 919 NSGTGSPSNANTPQALSRKSRRFMVYVHSKGMVVDDEYVVIGSANINQRSMEGTRDTEIA 978
Query: 701 IGCYQLENDGEELPNG--RDISTFRLSLWYEHTQRFEDLFLQPENLQCVQRVRSIADESW 750
+G YQ ++ +G I +R+SLW EH +D F QPE+++CV++VR++ + +W
Sbjct: 979 MGAYQPQHTWARKHSGPRGQIYGYRMSLWAEHMATLDDCFTQPESIECVRKVRTMGERNW 1038
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_038879261.1 | 0.0e+00 | 93.43 | phospholipase D alpha 4 [Benincasa hispida] | [more] |
| XP_008447602.1 | 0.0e+00 | 92.64 | PREDICTED: phospholipase D alpha 4 [Cucumis melo] >KAA0051141.1 phospholipase D ... | [more] |
| XP_023525777.1 | 0.0e+00 | 92.90 | phospholipase D alpha 4 [Cucurbita pepo subsp. pepo] | [more] |
| KAG6606722.1 | 0.0e+00 | 92.77 | Phospholipase D alpha 4, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
| KAG7036437.1 | 0.0e+00 | 92.90 | Phospholipase D alpha 4 [Cucurbita argyrosperma subsp. argyrosperma] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q9C888 | 1.2e-284 | 59.66 | Phospholipase D alpha 4 OS=Arabidopsis thaliana OX=3702 GN=PLDALPHA4 PE=2 SV=1 | [more] |
| Q43270 | 4.5e-178 | 41.11 | Phospholipase D alpha 1 OS=Zea mays OX=4577 GN=PLD1 PE=2 SV=1 | [more] |
| Q43007 | 7.6e-178 | 41.43 | Phospholipase D alpha 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PLD1 PE=1 SV... | [more] |
| P93400 | 1.2e-170 | 41.31 | Phospholipase D alpha 1 OS=Nicotiana tabacum OX=4097 GN=PLD1 PE=2 SV=2 | [more] |
| Q70EW5 | 1.5e-170 | 39.73 | Phospholipase D alpha 1 OS=Cynara cardunculus OX=4265 GN=PLD1 PE=1 SV=2 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A5D3BW81 | 0.0e+00 | 92.64 | Phospholipase D OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold863G0013... | [more] |
| A0A1S3BH90 | 0.0e+00 | 92.64 | Phospholipase D OS=Cucumis melo OX=3656 GN=LOC103490014 PE=3 SV=1 | [more] |
| A0A6J1G9V5 | 0.0e+00 | 92.77 | Phospholipase D OS=Cucurbita moschata OX=3662 GN=LOC111452112 PE=3 SV=1 | [more] |
| A0A6J1KAT1 | 0.0e+00 | 92.12 | Phospholipase D OS=Cucurbita maxima OX=3661 GN=LOC111492613 PE=3 SV=1 | [more] |
| A0A6J1D3G4 | 0.0e+00 | 91.33 | Phospholipase D OS=Momordica charantia OX=3673 GN=LOC111016958 PE=3 SV=1 | [more] |
