Spg023548 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg023548
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	adenylate isopentenyltransferase 5, chloroplastic-like
Location	scaffold13: 2551046 .. 2552020 (-)
RNA-Seq Expression	Spg023548
Synteny	Spg023548

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGAATTTCCTTGTCGGCGAGCTGCAAGCAAGCTGCGGCGGAGCCGCCGCTGGTAAACTTTCAAGGCACAAACACACTCAATTTGGAACCATTTTTCCGGCGAAAAAACAAAGTGGTGTTCGTAATGGGAGCCACCGGAACCGGAAAATCCCGGTTGGCAATCGATTTAGCCACCCGTTTCCCGGCGGAGATTATAAACTCCGACAAAATCCAAGTCTACAAAGGACTCGAAATCGTCACCAATAAGGTAACTGAAAAAGAATGTTGTGGCGTCCCACATCACCTGCTTGGAATCGCCGATCCAAACTCCAACTTCACCGCCGCCGACTTCCGCCGTCACGCCTCGTCGGCGATCAACTCCATCGTCGGAAAACACCGCCTCCCTATCGTCGCCGGCGGCTCCAACTCCTTCATGGAATCTCTCGTCGATGACGACGTCGAGTTTCGATACCGATACGACTGTTGCTTCCTGTGGGTCGACGTGTCGTTACCGATCCTCCACTCGTTCGTGTCAAAACGTGTTGATCGGATGTTGGACGACGGTTTTGTTAACGAGGTACGAAATTTGTTCGATCCTAAAGAAAATGATTACGTGCGTGGGATTCGACGCGCCATTGGAGTGCCTGAACTTGACGCGTTTCTACGAGCTGAGATGGATTCGGCTACTGATGATAGAGCTCGATCTCGGATTCTCGAGGCAGCCATTTTGAAGATGAAGGAAAATACAAGAAAATTGGCATTTCGACAATTGCAAAAGATTCGTCGCCTTCAAAACAAACGTGGATGGAGTGTCCACCGTATTGATGCCACTGAAGTCTTTTTGCAACGTGGCGATGGAGAATATTCTAACCAGGCTTGGGAGGCACTTGTGGCAGAGCCGAGCTCCATGATCGTAGACCAATTTCTCTATGAAAATAGTTGTTCCATAGCCGCTAATATTCCAATGGCAGTCGTAGCAGCTGCTACTCGATAA

mRNA sequence

ATGAGAATTTCCTTGTCGGCGAGCTGCAAGCAAGCTGCGGCGGAGCCGCCGCTGGTAAACTTTCAAGGCACAAACACACTCAATTTGGAACCATTTTTCCGGCGAAAAAACAAAGTGGTGTTCGTAATGGGAGCCACCGGAACCGGAAAATCCCGGTTGGCAATCGATTTAGCCACCCGTTTCCCGGCGGAGATTATAAACTCCGACAAAATCCAAGTCTACAAAGGACTCGAAATCGTCACCAATAAGGTAACTGAAAAAGAATGTTGTGGCGTCCCACATCACCTGCTTGGAATCGCCGATCCAAACTCCAACTTCACCGCCGCCGACTTCCGCCGTCACGCCTCGTCGGCGATCAACTCCATCGTCGGAAAACACCGCCTCCCTATCGTCGCCGGCGGCTCCAACTCCTTCATGGAATCTCTCGTCGATGACGACGTCGAGTTTCGATACCGATACGACTGTTGCTTCCTGTGGGTCGACGTGTCGTTACCGATCCTCCACTCGTTCGTGTCAAAACGTGTTGATCGGATGTTGGACGACGGTTTTGTTAACGAGGTACGAAATTTGTTCGATCCTAAAGAAAATGATTACGTGCGTGGGATTCGACGCGCCATTGGAGTGCCTGAACTTGACGCGTTTCTACGAGCTGAGATGGATTCGGCTACTGATGATAGAGCTCGATCTCGGATTCTCGAGGCAGCCATTTTGAAGATGAAGGAAAATACAAGAAAATTGGCATTTCGACAATTGCAAAAGATTCGTCGCCTTCAAAACAAACGTGGATGGAGTGTCCACCGTATTGATGCCACTGAAGTCTTTTTGCAACGTGGCGATGGAGAATATTCTAACCAGGCTTGGGAGGCACTTGTGGCAGAGCCGAGCTCCATGATCGTAGACCAATTTCTCTATGAAAATAGTTGTTCCATAGCCGCTAATATTCCAATGGCAGTCGTAGCAGCTGCTACTCGATAA

Coding sequence (CDS)

ATGAGAATTTCCTTGTCGGCGAGCTGCAAGCAAGCTGCGGCGGAGCCGCCGCTGGTAAACTTTCAAGGCACAAACACACTCAATTTGGAACCATTTTTCCGGCGAAAAAACAAAGTGGTGTTCGTAATGGGAGCCACCGGAACCGGAAAATCCCGGTTGGCAATCGATTTAGCCACCCGTTTCCCGGCGGAGATTATAAACTCCGACAAAATCCAAGTCTACAAAGGACTCGAAATCGTCACCAATAAGGTAACTGAAAAAGAATGTTGTGGCGTCCCACATCACCTGCTTGGAATCGCCGATCCAAACTCCAACTTCACCGCCGCCGACTTCCGCCGTCACGCCTCGTCGGCGATCAACTCCATCGTCGGAAAACACCGCCTCCCTATCGTCGCCGGCGGCTCCAACTCCTTCATGGAATCTCTCGTCGATGACGACGTCGAGTTTCGATACCGATACGACTGTTGCTTCCTGTGGGTCGACGTGTCGTTACCGATCCTCCACTCGTTCGTGTCAAAACGTGTTGATCGGATGTTGGACGACGGTTTTGTTAACGAGGTACGAAATTTGTTCGATCCTAAAGAAAATGATTACGTGCGTGGGATTCGACGCGCCATTGGAGTGCCTGAACTTGACGCGTTTCTACGAGCTGAGATGGATTCGGCTACTGATGATAGAGCTCGATCTCGGATTCTCGAGGCAGCCATTTTGAAGATGAAGGAAAATACAAGAAAATTGGCATTTCGACAATTGCAAAAGATTCGTCGCCTTCAAAACAAACGTGGATGGAGTGTCCACCGTATTGATGCCACTGAAGTCTTTTTGCAACGTGGCGATGGAGAATATTCTAACCAGGCTTGGGAGGCACTTGTGGCAGAGCCGAGCTCCATGATCGTAGACCAATTTCTCTATGAAAATAGTTGTTCCATAGCCGCTAATATTCCAATGGCAGTCGTAGCAGCTGCTACTCGATAA

Protein sequence

MRISLSASCKQAAAEPPLVNFQGTNTLNLEPFFRRKNKVVFVMGATGTGKSRLAIDLATRFPAEIINSDKIQVYKGLEIVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHASSAINSIVGKHRLPIVAGGSNSFMESLVDDDVEFRYRYDCCFLWVDVSLPILHSFVSKRVDRMLDDGFVNEVRNLFDPKENDYVRGIRRAIGVPELDAFLRAEMDSATDDRARSRILEAAILKMKENTRKLAFRQLQKIRRLQNKRGWSVHRIDATEVFLQRGDGEYSNQAWEALVAEPSSMIVDQFLYENSCSIAANIPMAVVAAATR

Homology

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BLAST of Spg023548 vs. NCBI nr
Match: KAG6575015.1 (Adenylate isopentenyltransferase 5, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 567.4 bits (1461), Expect = 8.0e-158
Identity = 280/324 (86.42%), Postives = 302/324 (93.21%), Query Frame = 0


Query: 1   MRISLSASCKQAAAEPPLVNFQGTNTLNLEPFFRRKNKVVFVMGATGTGKSRLAIDLATR 60
           M+ SLS SCKQ AAEPPLVNFQGTN LNL+PFFR+K+K+VFVMGATGTGKSRLAI+LA R
Sbjct: 1   MKFSLSVSCKQVAAEPPLVNFQGTNILNLKPFFRQKDKLVFVMGATGTGKSRLAIELANR 60

Query: 61  FPAEIINSDKIQVYKGLEIVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHASSAIN 120
           FPAEIINSDKIQ+YKGLEI+TNKVTEKECCGVPHHLLGIADPNSNFTA DFRRHAS AIN
Sbjct: 61  FPAEIINSDKIQIYKGLEILTNKVTEKECCGVPHHLLGIADPNSNFTATDFRRHASLAIN 120

Query: 121 SIVGKHRLPIVAGGSNSFMESLVDDDVEFRYRYDCCFLWVDVSLPILHSFVSKRVDRMLD 180
           SIVGKHRLPIVAGGSNSF+ESLVDD+ EFRYRYDCCFLWVDVSLP+LHSFVS RVDRMLD
Sbjct: 121 SIVGKHRLPIVAGGSNSFIESLVDDEAEFRYRYDCCFLWVDVSLPVLHSFVSDRVDRMLD 180

Query: 181 DGFVNEVRNLFDPKENDYVRGIRRAIGVPELDAFLRAEMDSATDDRARSRILEAAILKMK 240
           +G VNEVRNLFDPKENDY RGIRRAIGVPELDAFLRAEMDSATDD+ARSRIL+AAILK+K
Sbjct: 181 NGLVNEVRNLFDPKENDYTRGIRRAIGVPELDAFLRAEMDSATDDKARSRILKAAILKIK 240

Query: 241 ENTRKLAFRQLQKIRRLQNKRGWSVHRIDATEVFLQRGDGEYSNQAWEALVAEPSSMIVD 300
           EN+ KLA RQ QKI RLQ+K GW++HRIDATEVFLQ G GEYS+ AWEALVAEPSS+IVD
Sbjct: 241 ENSIKLALRQRQKIHRLQSKWGWNLHRIDATEVFLQHGGGEYSDHAWEALVAEPSSIIVD 300

Query: 301 QFLYENSCSIAANIPMAVVAAATR 325
           QFL ENSCS+AA IPMAVVA ATR
Sbjct: 301 QFLCENSCSLAAKIPMAVVAGATR 324

[Close]

BLAST of Spg023548 vs. NCBI nr
Match: KAG7013588.1 (Adenylate isopentenyltransferase 5, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 565.1 bits (1455), Expect = 4.0e-157
Identity = 279/324 (86.11%), Postives = 301/324 (92.90%), Query Frame = 0


Query: 1   MRISLSASCKQAAAEPPLVNFQGTNTLNLEPFFRRKNKVVFVMGATGTGKSRLAIDLATR 60
           M+ SLS SCKQ AAEPPLVNFQGTN LNL+PFFR+K+K+VFVMGATGTGKSRLAI+LA R
Sbjct: 1   MKFSLSVSCKQVAAEPPLVNFQGTNILNLKPFFRQKDKLVFVMGATGTGKSRLAIELANR 60

Query: 61  FPAEIINSDKIQVYKGLEIVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHASSAIN 120
           FPAEIINSDKIQ+YKGLEI+TNKVTEKECCGVPHHLLGIADPNSNFTA DFRRHAS AIN
Sbjct: 61  FPAEIINSDKIQIYKGLEILTNKVTEKECCGVPHHLLGIADPNSNFTATDFRRHASLAIN 120

Query: 121 SIVGKHRLPIVAGGSNSFMESLVDDDVEFRYRYDCCFLWVDVSLPILHSFVSKRVDRMLD 180
           SIVGKHRLPIVAGGSNSF+ESLVDD+ EFRYRYDCCFLWVDVSLP+LHSFVS RVDRMLD
Sbjct: 121 SIVGKHRLPIVAGGSNSFIESLVDDEAEFRYRYDCCFLWVDVSLPVLHSFVSDRVDRMLD 180

Query: 181 DGFVNEVRNLFDPKENDYVRGIRRAIGVPELDAFLRAEMDSATDDRARSRILEAAILKMK 240
           +G V EVRNLFDPKENDY RGIRRAIGVPELDAFLRAEMDSATDD+ARSRIL+AAILK+K
Sbjct: 181 NGLVKEVRNLFDPKENDYTRGIRRAIGVPELDAFLRAEMDSATDDKARSRILKAAILKIK 240

Query: 241 ENTRKLAFRQLQKIRRLQNKRGWSVHRIDATEVFLQRGDGEYSNQAWEALVAEPSSMIVD 300
           EN+ KLA RQ QKI RLQ+K GW++HRIDATEVFLQ G GEYS+ AWEALVAEPSS+IVD
Sbjct: 241 ENSIKLALRQRQKIHRLQSKWGWNLHRIDATEVFLQHGGGEYSDHAWEALVAEPSSIIVD 300

Query: 301 QFLYENSCSIAANIPMAVVAAATR 325
           QFL ENSCS+AA IPMAVVA ATR
Sbjct: 301 QFLCENSCSLAAKIPMAVVAGATR 324

[Close]

BLAST of Spg023548 vs. NCBI nr
Match: XP_023548308.1 (adenylate isopentenyltransferase 5, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 565.1 bits (1455), Expect = 4.0e-157
Identity = 278/324 (85.80%), Postives = 302/324 (93.21%), Query Frame = 0


Query: 1   MRISLSASCKQAAAEPPLVNFQGTNTLNLEPFFRRKNKVVFVMGATGTGKSRLAIDLATR 60
           M+ SLSASCKQ AAEPPLVNFQGTN LNL+PFFRRK+K+VFVMGATGTGKSRLAI+LA R
Sbjct: 1   MKFSLSASCKQVAAEPPLVNFQGTNILNLKPFFRRKDKLVFVMGATGTGKSRLAIELANR 60

Query: 61  FPAEIINSDKIQVYKGLEIVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHASSAIN 120
           FPAEIINSDKIQ+YKGLEI+TNKVTEKECCGVPHHLLGIADPNSNFTA DFRRHAS AIN
Sbjct: 61  FPAEIINSDKIQIYKGLEILTNKVTEKECCGVPHHLLGIADPNSNFTATDFRRHASLAIN 120

Query: 121 SIVGKHRLPIVAGGSNSFMESLVDDDVEFRYRYDCCFLWVDVSLPILHSFVSKRVDRMLD 180
           SIVGKHRLPIVAGGSNSF+ESLVD + EFRYRYDCCFLWVDVSLP+LHSFVS RVDRMLD
Sbjct: 121 SIVGKHRLPIVAGGSNSFIESLVDGEAEFRYRYDCCFLWVDVSLPVLHSFVSDRVDRMLD 180

Query: 181 DGFVNEVRNLFDPKENDYVRGIRRAIGVPELDAFLRAEMDSATDDRARSRILEAAILKMK 240
           +G VNEVRNLFDPKENDY RGIRRAIGVPELDAFLRAEMDSAT+D+ARSRIL+AAILK+K
Sbjct: 181 NGLVNEVRNLFDPKENDYTRGIRRAIGVPELDAFLRAEMDSATNDKARSRILKAAILKIK 240

Query: 241 ENTRKLAFRQLQKIRRLQNKRGWSVHRIDATEVFLQRGDGEYSNQAWEALVAEPSSMIVD 300
           EN+ KLA RQ QKI RLQ+K GW++HRIDATEVFLQ G GEYS+ AWEALVAEPSS+IVD
Sbjct: 241 ENSIKLALRQRQKIHRLQSKWGWNLHRIDATEVFLQHGGGEYSDHAWEALVAEPSSIIVD 300

Query: 301 QFLYENSCSIAANIPMAVVAAATR 325
           QFL ENSCS+AA IPMA++A ATR
Sbjct: 301 QFLCENSCSLAAKIPMAIIAGATR 324

[Close]

BLAST of Spg023548 vs. NCBI nr
Match: XP_022958992.1 (adenylate isopentenyltransferase 5, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 558.1 bits (1437), Expect = 4.8e-155
Identity = 276/324 (85.19%), Postives = 297/324 (91.67%), Query Frame = 0


Query: 1   MRISLSASCKQAAAEPPLVNFQGTNTLNLEPFFRRKNKVVFVMGATGTGKSRLAIDLATR 60
           M+ SLSASCKQ AAEPPLVN QGTN LNL+PFFR+K+K+VFVMGATGTGKSRLAI+LA R
Sbjct: 1   MKFSLSASCKQVAAEPPLVNLQGTNILNLKPFFRQKDKLVFVMGATGTGKSRLAIELANR 60

Query: 61  FPAEIINSDKIQVYKGLEIVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHASSAIN 120
           FPAEIINSDKIQ+YKGLEI+TNKVTEKECCGVPHHLLGIADPNSNFTA DFRRHAS AIN
Sbjct: 61  FPAEIINSDKIQIYKGLEILTNKVTEKECCGVPHHLLGIADPNSNFTATDFRRHASLAIN 120

Query: 121 SIVGKHRLPIVAGGSNSFMESLVDDDVEFRYRYDCCFLWVDVSLPILHSFVSKRVDRMLD 180
           SIVGKHRLPIVAGGSNSF+ESLVDD+ EFRYRYDCCFLWVDVSLP+LHSFVS RVDRMLD
Sbjct: 121 SIVGKHRLPIVAGGSNSFIESLVDDEAEFRYRYDCCFLWVDVSLPVLHSFVSDRVDRMLD 180

Query: 181 DGFVNEVRNLFDPKENDYVRGIRRAIGVPELDAFLRAEMDSATDDRARSRILEAAILKMK 240
           +G VNEVRNLFDPKENDY RGIRRAIGVPELDAFL AEMDSATDD+ARSRIL AAILK+K
Sbjct: 181 NGLVNEVRNLFDPKENDYTRGIRRAIGVPELDAFLHAEMDSATDDKARSRILNAAILKIK 240

Query: 241 ENTRKLAFRQLQKIRRLQNKRGWSVHRIDATEVFLQRGDGEYSNQAWEALVAEPSSMIVD 300
           EN+ KLA RQ QKI RLQ+K GW++HRIDATEVFLQ   GEYS+ AWEALVAEPSS+IVD
Sbjct: 241 ENSIKLALRQRQKIHRLQSKWGWNLHRIDATEVFLQHDGGEYSDHAWEALVAEPSSIIVD 300

Query: 301 QFLYENSCSIAANIPMAVVAAATR 325
           QFL ENSCS+A  IPMAVVA  TR
Sbjct: 301 QFLCENSCSLAGKIPMAVVARTTR 324

[Close]

BLAST of Spg023548 vs. NCBI nr
Match: XP_023006220.1 (adenylate isopentenyltransferase 5, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 551.6 bits (1420), Expect = 4.5e-153
Identity = 273/324 (84.26%), Postives = 296/324 (91.36%), Query Frame = 0


Query: 1   MRISLSASCKQAAAEPPLVNFQGTNTLNLEPFFRRKNKVVFVMGATGTGKSRLAIDLATR 60
           M+ SLSASCKQAA E PLVNFQGTN LNL+PFFRRK+K+VFVMGATGTGKSRLAI+LA R
Sbjct: 1   MKFSLSASCKQAATESPLVNFQGTNILNLKPFFRRKDKLVFVMGATGTGKSRLAIELANR 60

Query: 61  FPAEIINSDKIQVYKGLEIVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHASSAIN 120
           FPAEIINSDKIQ+YKGLEI+TNKVTEKECCGVPHHLLGI DPNSNFTA DF RHAS AIN
Sbjct: 61  FPAEIINSDKIQIYKGLEILTNKVTEKECCGVPHHLLGIVDPNSNFTATDFCRHASLAIN 120

Query: 121 SIVGKHRLPIVAGGSNSFMESLVDDDVEFRYRYDCCFLWVDVSLPILHSFVSKRVDRMLD 180
           SIVGKHRLPI+AGGSNSF+ESLVD + EFRYRYDCCFLWVDVSLP+LHSFVS RVDRMLD
Sbjct: 121 SIVGKHRLPIIAGGSNSFIESLVDGEAEFRYRYDCCFLWVDVSLPVLHSFVSDRVDRMLD 180

Query: 181 DGFVNEVRNLFDPKENDYVRGIRRAIGVPELDAFLRAEMDSATDDRARSRILEAAILKMK 240
           +G VNEVRNLFDPK NDY RGIRRAIGVPELDAFLRAEMDSATD++ARS+IL+AAILK+K
Sbjct: 181 NGLVNEVRNLFDPKGNDYTRGIRRAIGVPELDAFLRAEMDSATDNKARSQILQAAILKIK 240

Query: 241 ENTRKLAFRQLQKIRRLQNKRGWSVHRIDATEVFLQRGDGEYSNQAWEALVAEPSSMIVD 300
           EN+ KLA RQ QKI  LQ+K GW++HRIDATEVFLQ G GEYS  AWEALVAEPSS+IVD
Sbjct: 241 ENSIKLALRQRQKIHHLQSKWGWNLHRIDATEVFLQHGGGEYSEHAWEALVAEPSSIIVD 300

Query: 301 QFLYENSCSIAANIPMAVVAAATR 325
           QFL ENSCS+AA IPMAVVA ATR
Sbjct: 301 QFLCENSCSLAAKIPMAVVAGATR 324

[Close]

BLAST of Spg023548 vs. ExPASy Swiss-Prot
Match: Q94ID2 (Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT5 PE=1 SV=2)

HSP 1 Score: 361.7 bits (927), Expect = 8.7e-99
Identity = 187/290 (64.48%), Postives = 235/290 (81.03%), Query Frame = 0


Query: 15  EPPLVNFQGTNTLNLEPFFRRKNKVVFVMGATGTGKSRLAIDLATRFPAEIINSDKIQVY 74
           +P  +NFQG N +++ PFFRRK+KVVFVMGATGTGKSRLAIDLATRFPAEI+NSDKIQVY
Sbjct: 13  QPLSLNFQG-NMVDV-PFFRRKDKVVFVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVY 72

Query: 75  KGLEIVTNKVTEKECCGVPHHLLG-IADPNSNFTAADFRRHASSAINSIVGKHRLPIVAG 134
           KGL+IVTNKVT +E  GVPHHLLG + D   +FTA DF+R A  A+ SIV + R+PI+AG
Sbjct: 73  KGLDIVTNKVTPEESLGVPHHLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPIIAG 132

Query: 135 GSNSFMESLVDDDVEFRYRYDCCFLWVDVSLPILHSFVSKRVDRMLDDGFVNEVRNLFDP 194
           GSNS++E+LV+D V+FR RY+CCFLWVDVS P+LHSFVS+RVD+M+D G V+EVR +FDP
Sbjct: 133 GSNSYIEALVNDCVDFRLRYNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIFDP 192

Query: 195 KENDYVRGIRRAIGVPELDAFLRAEMDSATDDRARSRILEAAILKMKENTRKLAFRQLQK 254
             +DY  GIRRAIGVPELD FLR+EM +   +    R+LE AI K+KENT  LA RQLQK
Sbjct: 193 SSSDYSAGIRRAIGVPELDEFLRSEMRNYPAE-TTERLLETAIEKIKENTCLLACRQLQK 252

Query: 255 IRRLQNKRGWSVHRIDATEVFLQRGDGEYSNQAWEALVAEPSSMIVDQFL 304
           I+RL  +  W++HR+DATEVFL+R  GE +++AW+  VA PS++ V++FL
Sbjct: 253 IQRLYKQWKWNMHRVDATEVFLRR--GEEADEAWDNSVAHPSALAVEKFL 297

[Close]

BLAST of Spg023548 vs. ExPASy Swiss-Prot
Match: Q94ID1 (Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IPT7 PE=2 SV=2)

HSP 1 Score: 316.6 bits (810), Expect = 3.2e-85
Identity = 174/309 (56.31%), Postives = 223/309 (72.17%), Query Frame = 0


Query: 1   MRISLSASCKQAAAEPPLVNFQGTNTLNLEPFFRRKNKVVFVMGATGTGKSRLAIDLATR 60
           M+ S+S S KQ   +P L      + +N+  F   K KV+FVMGATG+GKSRLAIDLATR
Sbjct: 1   MKFSIS-SLKQ--VQPILCFKNKLSKVNVNSFLHPKEKVIFVMGATGSGKSRLAIDLATR 60

Query: 61  FPAEIINSDKIQVYKGLEIVTNKVTEKECCGVPHHLLGIADPNS-NFTAADFRRHASSAI 120
           F  EIINSDKIQ+YKGL+++TNKVT KEC GVPHHLLG+ D  + N TA  + R AS AI
Sbjct: 61  FQGEIINSDKIQLYKGLDVLTNKVTPKECRGVPHHLLGVFDSEAGNLTATQYSRLASQAI 120

Query: 121 NSIVGKHRLPIVAGGSNSFMESLVDDDVEFRY-RYDCCFLWVDVSLPILHSFVSKRVDRM 180
           + +   ++LPIVAGGSNS++E+LV+    F    YDCCF+WVDVSLP+L+SFVSKRVDRM
Sbjct: 121 SKLSANNKLPIVAGGSNSYIEALVNHSSGFLLNNYDCCFIWVDVSLPVLNSFVSKRVDRM 180

Query: 181 LDDGFVNEVRNLFDPKENDYVRGIRRAIGVPELDAFLRAEMDSATDDRARSRILEAAILK 240
           ++ G + EVR +F+PK N Y  GIRRAIGVPEL  +LR E  S  D   +S++L+ A+  
Sbjct: 181 MEAGLLEEVREVFNPKAN-YSVGIRRAIGVPELHEYLRNE--SLVDRATKSKMLDVAVKN 240

Query: 241 MKENTRKLAFRQLQKIRRLQNKRGWSVHRIDATEVFLQRGDGEYSNQAWEALVAEPSSMI 300
           +K+NT  LA RQL+KI+RL  K   S+HR+DATEVFL+R + E  ++AWE LVA PS  I
Sbjct: 241 IKKNTEILACRQLKKIQRLHKKWKMSMHRVDATEVFLKR-NVEEQDEAWENLVARPSERI 300

Query: 301 VDQFLYENS 308
           VD+F   N+
Sbjct: 301 VDKFYNNNN 302

[Close]

BLAST of Spg023548 vs. ExPASy Swiss-Prot
Match: Q93WC9 (Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT3 PE=1 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 7.2e-85
Identity = 167/327 (51.07%), Postives = 227/327 (69.42%), Query Frame = 0


Query: 1   MRISLSASCKQAAAEPPLVNFQ----GTNTLNLEPFFRRKNKVVFVMGATGTGKSRLAID 60
           M+IS+ A CKQ     P ++F     G N L L P +  K+KVV +MGATGTGKSRL++D
Sbjct: 3   MKISM-AMCKQPLPPSPTLDFPPARFGPNMLTLNP-YGPKDKVVVIMGATGTGKSRLSVD 62

Query: 61  LATRFPAEIINSDKIQVYKGLEIVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHAS 120
           +ATRF AEIINSDKIQV++GL+IVTNK+T +E CGVPHHLLG+  P ++ TAA++   A+
Sbjct: 63  IATRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHHLLGVLPPEADLTAANYCHMAN 122

Query: 121 SAINSIVGKHRLPIVAGGSNSFMESLVDD-DVEFRYRYDCCFLWVDVSLPILHSFVSKRV 180
            +I S++ + +LPI+ GGSNS++E+LVDD + +FR RYDCCFLWVDV+LP+LH FVS+RV
Sbjct: 123 LSIESVLNRGKLPIIVGGSNSYVEALVDDKENKFRSRYDCCFLWVDVALPVLHGFVSERV 182

Query: 181 DRMLDDGFVNEVRNLFDPKENDYVRGIRRAIGVPELDAFLRAEMDSATDDRARSRILEAA 240
           D+M++ G V EVR  FD   +DY RGI++AIG PE D F R E     +D  R  +L   
Sbjct: 183 DKMVESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDRFFRNEQFLNVED--REELLSKV 242

Query: 241 ILKMKENTRKLAFRQLQKIRRLQNKRGWSVHRIDATEVFLQRGDGEYSNQAWEALVAEPS 300
           + ++K NT +LA RQ +KI RL+  + WS+ R+DAT VF +R     +N AWE LVA PS
Sbjct: 243 LEEIKRNTFELACRQREKIERLRKVKKWSIQRVDATPVFTKRRSKMDANVAWERLVAGPS 302

Query: 301 SMIVDQFLYENSCSIAANIPMAVVAAA 323
           +  V +FL +    IA+  P+   + A
Sbjct: 303 TDTVSRFLLD----IASRRPLVEASTA 321

[Close]

BLAST of Spg023548 vs. ExPASy Swiss-Prot
Match: Q5GHF7 (Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 1.2e-63
Identity = 139/314 (44.27%), Postives = 185/314 (58.92%), Query Frame = 0


Query: 24  TNTLNLEPFFRRKNKVVFVMGATGTGKSRLAIDLATRFPAEIINSDKIQVYKGLEIVTNK 83
           T  ++L     RK K++ +MGATGTGKSRL+IDLA  FP E+INSDK+QVYKGL+I TNK
Sbjct: 17  TTNVSLRRQRHRKEKLLVLMGATGTGKSRLSIDLAAHFPLEVINSDKMQVYKGLDITTNK 76

Query: 84  VTEKECCGVPHHLLGIADP-NSNFTAADFRRHASSAINSIVGKHRLPIVAGGSNSFMESL 143
           ++  +  GVPHHLLG  DP     T ADFR  A  A++ I G+ +LP++ GGSNSF+ +L
Sbjct: 77  ISVPDRGGVPHHLLGEVDPARGELTPADFRSLAGKAVSEITGRRKLPVLVGGSNSFIHAL 136

Query: 144 VDDD----------------VEFRYRYDCCFLWVDVSLPILHSFVSKRVDRMLDDGFVNE 203
           + D                 V    RYDCCFLWVDVS+ +L  +++KRVD ML+ G  +E
Sbjct: 137 LVDRFDSSGPGVFEEGSHSVVSSELRYDCCFLWVDVSVKVLTDYLAKRVDDMLELGMFDE 196

Query: 204 VRNLFDPKENDYVR------GIRRAIGVPELDAFLR------AEMDSATDDRARSRILEA 263
           +   + P++ D+        G+R+AIGVPE D +         E +    DR R    E 
Sbjct: 197 LAEFYSPEDEDHDEDSATRTGLRKAIGVPEFDRYFEKFRPGDVEGEDPGRDRVRRGAFEE 256

Query: 264 AILKMKENTRKLAFRQLQKIRRLQNKRGWSVHRIDATEVF---LQRGDGEYSNQAWEALV 306
           A+  +KENT  LA RQ+ KI RL+   GW + R+DATE F   +    GE   + WE  V
Sbjct: 257 AVRAIKENTCHLAKRQIGKILRLKG-AGWDLRRLDATESFRAAMTSDSGEKCTEIWEKQV 316

[Close]

BLAST of Spg023548 vs. ExPASy Swiss-Prot
Match: Q94ID3 (Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT1 PE=1 SV=2)

HSP 1 Score: 232.3 bits (591), Expect = 8.0e-60
Identity = 126/296 (42.57%), Postives = 183/296 (61.82%), Query Frame = 0


Query: 35  RKNKVVFVMGATGTGKSRLAIDLATRFPAEIINSDKIQVYKGLEIVTNKVTEKECCGVPH 94
           RK+KVV ++GATG GKSRL++DLATRFP+EIINSDKIQVY+GLEI TN++T ++  GVPH
Sbjct: 63  RKDKVVVILGATGAGKSRLSVDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVPH 122

Query: 95  HLLGIADP-NSNFTAADFRRHASSAINSIVGKHRLPIVAGGSNSFMESLVDDDVEFRY-- 154
           HLLG+ +P +   TA +FR  AS+ +  I  + ++PI+AGGSNSF+ +L+    + ++  
Sbjct: 123 HLLGVINPEHGELTAGEFRSAASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFDP 182

Query: 155 ------------RYDCCFLWVDVSLPILHSFVSKRVDRMLDDGFVNEVRNLFDPKENDYV 214
                       RY+CCF+WVDVS  +L+ ++ +RVD M+D G   E+   +DP ++   
Sbjct: 183 FSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGLE 242

Query: 215 R--GIRRAIGVPELDAFLR---AEMDSATDDRARSRILEAAILKMKENTRKLAFRQLQKI 274
              GIR+AIGVPE D + +    E      D  R    + A+  +K NT  LA RQ++KI
Sbjct: 243 TRFGIRKAIGVPEFDGYFKEYPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKKI 302

Query: 275 RRLQNKRGWSVHRIDAT----EVFLQRGDGEYSNQAWEALVAEPSSMIVDQFLYEN 307
             L++  GW + R+DAT     V ++    +   + WE  V EPS  IV + L +N
Sbjct: 303 EMLKD-AGWEIERVDATASFKAVMMKSSSEKKWRENWEEQVLEPSVKIVKRHLVQN 357

[Close]

BLAST of Spg023548 vs. ExPASy TrEMBL
Match: A0A6J1H3C2 (adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111460117 PE=4 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 2.3e-155
Identity = 276/324 (85.19%), Postives = 297/324 (91.67%), Query Frame = 0


Query: 1   MRISLSASCKQAAAEPPLVNFQGTNTLNLEPFFRRKNKVVFVMGATGTGKSRLAIDLATR 60
           M+ SLSASCKQ AAEPPLVN QGTN LNL+PFFR+K+K+VFVMGATGTGKSRLAI+LA R
Sbjct: 1   MKFSLSASCKQVAAEPPLVNLQGTNILNLKPFFRQKDKLVFVMGATGTGKSRLAIELANR 60

Query: 61  FPAEIINSDKIQVYKGLEIVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHASSAIN 120
           FPAEIINSDKIQ+YKGLEI+TNKVTEKECCGVPHHLLGIADPNSNFTA DFRRHAS AIN
Sbjct: 61  FPAEIINSDKIQIYKGLEILTNKVTEKECCGVPHHLLGIADPNSNFTATDFRRHASLAIN 120

Query: 121 SIVGKHRLPIVAGGSNSFMESLVDDDVEFRYRYDCCFLWVDVSLPILHSFVSKRVDRMLD 180
           SIVGKHRLPIVAGGSNSF+ESLVDD+ EFRYRYDCCFLWVDVSLP+LHSFVS RVDRMLD
Sbjct: 121 SIVGKHRLPIVAGGSNSFIESLVDDEAEFRYRYDCCFLWVDVSLPVLHSFVSDRVDRMLD 180

Query: 181 DGFVNEVRNLFDPKENDYVRGIRRAIGVPELDAFLRAEMDSATDDRARSRILEAAILKMK 240
           +G VNEVRNLFDPKENDY RGIRRAIGVPELDAFL AEMDSATDD+ARSRIL AAILK+K
Sbjct: 181 NGLVNEVRNLFDPKENDYTRGIRRAIGVPELDAFLHAEMDSATDDKARSRILNAAILKIK 240

Query: 241 ENTRKLAFRQLQKIRRLQNKRGWSVHRIDATEVFLQRGDGEYSNQAWEALVAEPSSMIVD 300
           EN+ KLA RQ QKI RLQ+K GW++HRIDATEVFLQ   GEYS+ AWEALVAEPSS+IVD
Sbjct: 241 ENSIKLALRQRQKIHRLQSKWGWNLHRIDATEVFLQHDGGEYSDHAWEALVAEPSSIIVD 300

Query: 301 QFLYENSCSIAANIPMAVVAAATR 325
           QFL ENSCS+A  IPMAVVA  TR
Sbjct: 301 QFLCENSCSLAGKIPMAVVARTTR 324

[Close]

BLAST of Spg023548 vs. ExPASy TrEMBL
Match: A0A6J1KZJ7 (adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111499016 PE=4 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 2.2e-153
Identity = 273/324 (84.26%), Postives = 296/324 (91.36%), Query Frame = 0


Query: 1   MRISLSASCKQAAAEPPLVNFQGTNTLNLEPFFRRKNKVVFVMGATGTGKSRLAIDLATR 60
           M+ SLSASCKQAA E PLVNFQGTN LNL+PFFRRK+K+VFVMGATGTGKSRLAI+LA R
Sbjct: 1   MKFSLSASCKQAATESPLVNFQGTNILNLKPFFRRKDKLVFVMGATGTGKSRLAIELANR 60

Query: 61  FPAEIINSDKIQVYKGLEIVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHASSAIN 120
           FPAEIINSDKIQ+YKGLEI+TNKVTEKECCGVPHHLLGI DPNSNFTA DF RHAS AIN
Sbjct: 61  FPAEIINSDKIQIYKGLEILTNKVTEKECCGVPHHLLGIVDPNSNFTATDFCRHASLAIN 120

Query: 121 SIVGKHRLPIVAGGSNSFMESLVDDDVEFRYRYDCCFLWVDVSLPILHSFVSKRVDRMLD 180
           SIVGKHRLPI+AGGSNSF+ESLVD + EFRYRYDCCFLWVDVSLP+LHSFVS RVDRMLD
Sbjct: 121 SIVGKHRLPIIAGGSNSFIESLVDGEAEFRYRYDCCFLWVDVSLPVLHSFVSDRVDRMLD 180

Query: 181 DGFVNEVRNLFDPKENDYVRGIRRAIGVPELDAFLRAEMDSATDDRARSRILEAAILKMK 240
           +G VNEVRNLFDPK NDY RGIRRAIGVPELDAFLRAEMDSATD++ARS+IL+AAILK+K
Sbjct: 181 NGLVNEVRNLFDPKGNDYTRGIRRAIGVPELDAFLRAEMDSATDNKARSQILQAAILKIK 240

Query: 241 ENTRKLAFRQLQKIRRLQNKRGWSVHRIDATEVFLQRGDGEYSNQAWEALVAEPSSMIVD 300
           EN+ KLA RQ QKI  LQ+K GW++HRIDATEVFLQ G GEYS  AWEALVAEPSS+IVD
Sbjct: 241 ENSIKLALRQRQKIHHLQSKWGWNLHRIDATEVFLQHGGGEYSEHAWEALVAEPSSIIVD 300

Query: 301 QFLYENSCSIAANIPMAVVAAATR 325
           QFL ENSCS+AA IPMAVVA ATR
Sbjct: 301 QFLCENSCSLAAKIPMAVVAGATR 324

[Close]

BLAST of Spg023548 vs. ExPASy TrEMBL
Match: A0A6J1EWK5 (adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111436795 PE=4 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 1.4e-147
Identity = 258/319 (80.88%), Postives = 294/319 (92.16%), Query Frame = 0


Query: 1   MRISLSASCKQAAAEPPLVNFQGTNTLNLEPFFRRKNKVVFVMGATGTGKSRLAIDLATR 60
           M+ISLSASCK+AAAEPPL++FQGTNT+NL+PFFR+K+K+VFVMGATGTGKSRLA+DLATR
Sbjct: 1   MKISLSASCKKAAAEPPLIDFQGTNTVNLKPFFRQKDKLVFVMGATGTGKSRLAVDLATR 60

Query: 61  FPAEIINSDKIQVYKGLEIVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHASSAIN 120
           FPAEI+NSDKIQ+YKGLE+VTNKVTEKECCGVPHHLLGIADPNSNFT ADFRRHA+S IN
Sbjct: 61  FPAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTFADFRRHATSTIN 120

Query: 121 SIVGKHRLPIVAGGSNSFMESLVDDDVEFRYRYDCCFLWVDVSLPILHSFVSKRVDRMLD 180
           SIVGKHRLPIVAGGSNSF+ESLVD D EFRY+Y+CCFLWVDVSLPILHSFVS+RVDRMLD
Sbjct: 121 SIVGKHRLPIVAGGSNSFIESLVDGDAEFRYQYECCFLWVDVSLPILHSFVSERVDRMLD 180

Query: 181 DGFVNEVRNLFDPKENDYVRGIRRAIGVPELDAFLRAEMDSATDDRARSRILEAAILKMK 240
           +GF+NEVRNLFDP ENDY RGIRRAIGVPELDAFLRAEMDSATD RARSRILEAAILK+K
Sbjct: 181 NGFINEVRNLFDPNENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARSRILEAAILKIK 240

Query: 241 ENTRKLAFRQLQKIRRLQNKRGWSVHRIDATEVFLQRGDGEYSNQAWEALVAEPSSMIVD 300
           ENTRKLAFRQ Q IRRL++KRGW++HRIDATEVF  R + ++S+  WEALV EP+S I+ 
Sbjct: 241 ENTRKLAFRQWQNIRRLRSKRGWNLHRIDATEVFQHRDNKQHSDHVWEALVTEPASKILH 300

Query: 301 QFLYENSCSIAANIPMAVV 320
           QFL +N+ ++  NIP   +
Sbjct: 301 QFLCQNTYTV--NIPTTAI 317

[Close]

BLAST of Spg023548 vs. ExPASy TrEMBL
Match: A0A6J1KF69 (adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111494482 PE=4 SV=1)

HSP 1 Score: 506.5 bits (1303), Expect = 8.1e-140
Identity = 247/319 (77.43%), Postives = 283/319 (88.71%), Query Frame = 0


Query: 1   MRISLSASCKQAAAEPPLVNFQGTNTLNLEPFFRRKNKVVFVMGATGTGKSRLAIDLATR 60
           M+ISLSASCK+AAAEPPL++FQ  NT+NL+PFFR+K+K+VFVMGATGTGKSRLA++LATR
Sbjct: 38  MKISLSASCKKAAAEPPLIDFQRRNTINLKPFFRQKDKLVFVMGATGTGKSRLAVNLATR 97

Query: 61  FPAEIINSDKIQVYKGLEIVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHASSAIN 120
           FPAEI+NSDKIQ+YKGLE+VTNKVTEKECCGVPHHLLGIADPNSNFTA DFR  A S IN
Sbjct: 98  FPAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAVDFRHVAMSTIN 157

Query: 121 SIVGKHRLPIVAGGSNSFMESLVDDDVEFRYRYDCCFLWVDVSLPILHSFVSKRVDRMLD 180
           SI+G+HRLPIVAGGSNSF+ESLVD D EFRY+Y+CCFLW+DVSLPILHSFVS+RVDRMLD
Sbjct: 158 SIIGRHRLPIVAGGSNSFIESLVDGDAEFRYQYECCFLWIDVSLPILHSFVSERVDRMLD 217

Query: 181 DGFVNEVRNLFDPKENDYVRGIRRAIGVPELDAFLRAEMDSATDDRARSRILEAAILKMK 240
           +GF+NEVRNLFD  ENDY RGIRRAIGVPELDAFLRAEMDSATD RARSRILEAAILK K
Sbjct: 218 NGFINEVRNLFDSNENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARSRILEAAILKTK 277

Query: 241 ENTRKLAFRQLQKIRRLQNKRGWSVHRIDATEVFLQRGDGEYSNQAWEALVAEPSSMIVD 300
           ENTRKLAFRQ Q I RL+ KRGW++HRIDATEVF  R + +  +  WEALV EP+S I+ 
Sbjct: 278 ENTRKLAFRQWQNICRLRRKRGWNLHRIDATEVFQHRDNEQQFDHVWEALVTEPASKILH 337

Query: 301 QFLYENSCSIAANIPMAVV 320
           QFL +N+ ++  NIP   +
Sbjct: 338 QFLCQNTYTV--NIPTTTI 354

[Close]

BLAST of Spg023548 vs. ExPASy TrEMBL
Match: A0A5A7STV9 (Adenylate isopentenyltransferase 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold111G00780 PE=4 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 1.1e-136
Identity = 262/327 (80.12%), Postives = 286/327 (87.46%), Query Frame = 0


Query: 1   MRISLSASCKQAAAEPPLVNFQGT--NTLNLEPFFRRKNKVVFVMGATGTGKSRLAIDLA 60
           M+I LS SCKQ A EPPLVNFQGT  NTLNLEPFFRRK+KVVFVMGATGTGKSRLAIDLA
Sbjct: 1   MKIWLSTSCKQVAVEPPLVNFQGTNNNTLNLEPFFRRKDKVVFVMGATGTGKSRLAIDLA 60

Query: 61  TRFPAEIINSDKIQVYKGLEIVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHASSA 120
           TRFPAEIINSDKIQVYKGL+++TNKVTEKEC GVPHHLLGIADPNSNFT ADFRRHASSA
Sbjct: 61  TRFPAEIINSDKIQVYKGLKVLTNKVTEKECRGVPHHLLGIADPNSNFTVADFRRHASSA 120

Query: 121 INS-IVGKHRLPIVAGGSNSFMESLVDDDVEFRYRYDCCFLWVDVSLPILHSFVSKRVDR 180
           INS IVGK RLPI+AGGSNSF+ESLV+++ EFRYRYDCCFLWVDVSLPIL+SFVS+RVDR
Sbjct: 121 INSIIVGKRRLPILAGGSNSFIESLVEEEPEFRYRYDCCFLWVDVSLPILNSFVSERVDR 180

Query: 181 MLDDGFVNEVRNLFDPK--ENDYVRGIRRAIGVPELDAFLRAEMDSATDDRARSRILEAA 240
           ML++G VNEVRNLFDP    NDY RGIRRAIGVPELDAFLRAEMDS TDD+ARSRIL+AA
Sbjct: 181 MLENGLVNEVRNLFDPNGLGNDYGRGIRRAIGVPELDAFLRAEMDSTTDDKARSRILKAA 240

Query: 241 ILKMKENTRKLAFRQLQKIRRLQNK-RGW-SVHRIDATEVFLQRGDGE--YSNQAWEALV 300
           ILK+KENT KLA RQ QKI RLQNK RGW + HRIDATE FLQ G+GE   S+ AWE LV
Sbjct: 241 ILKIKENTIKLACRQQQKIHRLQNKWRGWNNFHRIDATEAFLQLGNGENYSSDNAWETLV 300

Query: 301 AEPSSMIVDQFLYENSCSIAANIPMAV 319
            EPSSMI+DQFL +NS  +   +  AV
Sbjct: 301 VEPSSMIIDQFLSKNSFKLTEKVFNAV 327

[Close]

BLAST of Spg023548 vs. TAIR 10
Match: AT5G19040.1 (isopentenyltransferase 5 )

HSP 1 Score: 361.7 bits (927), Expect = 6.2e-100
Identity = 187/290 (64.48%), Postives = 235/290 (81.03%), Query Frame = 0


Query: 15  EPPLVNFQGTNTLNLEPFFRRKNKVVFVMGATGTGKSRLAIDLATRFPAEIINSDKIQVY 74
           +P  +NFQG N +++ PFFRRK+KVVFVMGATGTGKSRLAIDLATRFPAEI+NSDKIQVY
Sbjct: 13  QPLSLNFQG-NMVDV-PFFRRKDKVVFVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVY 72

Query: 75  KGLEIVTNKVTEKECCGVPHHLLG-IADPNSNFTAADFRRHASSAINSIVGKHRLPIVAG 134
           KGL+IVTNKVT +E  GVPHHLLG + D   +FTA DF+R A  A+ SIV + R+PI+AG
Sbjct: 73  KGLDIVTNKVTPEESLGVPHHLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPIIAG 132

Query: 135 GSNSFMESLVDDDVEFRYRYDCCFLWVDVSLPILHSFVSKRVDRMLDDGFVNEVRNLFDP 194
           GSNS++E+LV+D V+FR RY+CCFLWVDVS P+LHSFVS+RVD+M+D G V+EVR +FDP
Sbjct: 133 GSNSYIEALVNDCVDFRLRYNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIFDP 192

Query: 195 KENDYVRGIRRAIGVPELDAFLRAEMDSATDDRARSRILEAAILKMKENTRKLAFRQLQK 254
             +DY  GIRRAIGVPELD FLR+EM +   +    R+LE AI K+KENT  LA RQLQK
Sbjct: 193 SSSDYSAGIRRAIGVPELDEFLRSEMRNYPAE-TTERLLETAIEKIKENTCLLACRQLQK 252

Query: 255 IRRLQNKRGWSVHRIDATEVFLQRGDGEYSNQAWEALVAEPSSMIVDQFL 304
           I+RL  +  W++HR+DATEVFL+R  GE +++AW+  VA PS++ V++FL
Sbjct: 253 IQRLYKQWKWNMHRVDATEVFLRR--GEEADEAWDNSVAHPSALAVEKFL 297

[Close]

BLAST of Spg023548 vs. TAIR 10
Match: AT3G23630.1 (isopentenyltransferase 7 )

HSP 1 Score: 316.6 bits (810), Expect = 2.3e-86
Identity = 174/309 (56.31%), Postives = 223/309 (72.17%), Query Frame = 0


Query: 1   MRISLSASCKQAAAEPPLVNFQGTNTLNLEPFFRRKNKVVFVMGATGTGKSRLAIDLATR 60
           M+ S+S S KQ   +P L      + +N+  F   K KV+FVMGATG+GKSRLAIDLATR
Sbjct: 1   MKFSIS-SLKQ--VQPILCFKNKLSKVNVNSFLHPKEKVIFVMGATGSGKSRLAIDLATR 60

Query: 61  FPAEIINSDKIQVYKGLEIVTNKVTEKECCGVPHHLLGIADPNS-NFTAADFRRHASSAI 120
           F  EIINSDKIQ+YKGL+++TNKVT KEC GVPHHLLG+ D  + N TA  + R AS AI
Sbjct: 61  FQGEIINSDKIQLYKGLDVLTNKVTPKECRGVPHHLLGVFDSEAGNLTATQYSRLASQAI 120

Query: 121 NSIVGKHRLPIVAGGSNSFMESLVDDDVEFRY-RYDCCFLWVDVSLPILHSFVSKRVDRM 180
           + +   ++LPIVAGGSNS++E+LV+    F    YDCCF+WVDVSLP+L+SFVSKRVDRM
Sbjct: 121 SKLSANNKLPIVAGGSNSYIEALVNHSSGFLLNNYDCCFIWVDVSLPVLNSFVSKRVDRM 180

Query: 181 LDDGFVNEVRNLFDPKENDYVRGIRRAIGVPELDAFLRAEMDSATDDRARSRILEAAILK 240
           ++ G + EVR +F+PK N Y  GIRRAIGVPEL  +LR E  S  D   +S++L+ A+  
Sbjct: 181 MEAGLLEEVREVFNPKAN-YSVGIRRAIGVPELHEYLRNE--SLVDRATKSKMLDVAVKN 240

Query: 241 MKENTRKLAFRQLQKIRRLQNKRGWSVHRIDATEVFLQRGDGEYSNQAWEALVAEPSSMI 300
           +K+NT  LA RQL+KI+RL  K   S+HR+DATEVFL+R + E  ++AWE LVA PS  I
Sbjct: 241 IKKNTEILACRQLKKIQRLHKKWKMSMHRVDATEVFLKR-NVEEQDEAWENLVARPSERI 300

Query: 301 VDQFLYENS 308
           VD+F   N+
Sbjct: 301 VDKFYNNNN 302

[Close]

BLAST of Spg023548 vs. TAIR 10
Match: AT3G63110.1 (isopentenyltransferase 3 )

HSP 1 Score: 315.5 bits (807), Expect = 5.1e-86
Identity = 167/327 (51.07%), Postives = 227/327 (69.42%), Query Frame = 0


Query: 1   MRISLSASCKQAAAEPPLVNFQ----GTNTLNLEPFFRRKNKVVFVMGATGTGKSRLAID 60
           M+IS+ A CKQ     P ++F     G N L L P +  K+KVV +MGATGTGKSRL++D
Sbjct: 3   MKISM-AMCKQPLPPSPTLDFPPARFGPNMLTLNP-YGPKDKVVVIMGATGTGKSRLSVD 62

Query: 61  LATRFPAEIINSDKIQVYKGLEIVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHAS 120
           +ATRF AEIINSDKIQV++GL+IVTNK+T +E CGVPHHLLG+  P ++ TAA++   A+
Sbjct: 63  IATRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHHLLGVLPPEADLTAANYCHMAN 122

Query: 121 SAINSIVGKHRLPIVAGGSNSFMESLVDD-DVEFRYRYDCCFLWVDVSLPILHSFVSKRV 180
            +I S++ + +LPI+ GGSNS++E+LVDD + +FR RYDCCFLWVDV+LP+LH FVS+RV
Sbjct: 123 LSIESVLNRGKLPIIVGGSNSYVEALVDDKENKFRSRYDCCFLWVDVALPVLHGFVSERV 182

Query: 181 DRMLDDGFVNEVRNLFDPKENDYVRGIRRAIGVPELDAFLRAEMDSATDDRARSRILEAA 240
           D+M++ G V EVR  FD   +DY RGI++AIG PE D F R E     +D  R  +L   
Sbjct: 183 DKMVESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDRFFRNEQFLNVED--REELLSKV 242

Query: 241 ILKMKENTRKLAFRQLQKIRRLQNKRGWSVHRIDATEVFLQRGDGEYSNQAWEALVAEPS 300
           + ++K NT +LA RQ +KI RL+  + WS+ R+DAT VF +R     +N AWE LVA PS
Sbjct: 243 LEEIKRNTFELACRQREKIERLRKVKKWSIQRVDATPVFTKRRSKMDANVAWERLVAGPS 302

Query: 301 SMIVDQFLYENSCSIAANIPMAVVAAA 323
           +  V +FL +    IA+  P+   + A
Sbjct: 303 TDTVSRFLLD----IASRRPLVEASTA 321

[Close]

BLAST of Spg023548 vs. TAIR 10
Match: AT1G68460.1 (isopentenyltransferase 1 )

HSP 1 Score: 232.3 bits (591), Expect = 5.7e-61
Identity = 126/296 (42.57%), Postives = 183/296 (61.82%), Query Frame = 0


Query: 35  RKNKVVFVMGATGTGKSRLAIDLATRFPAEIINSDKIQVYKGLEIVTNKVTEKECCGVPH 94
           RK+KVV ++GATG GKSRL++DLATRFP+EIINSDKIQVY+GLEI TN++T ++  GVPH
Sbjct: 63  RKDKVVVILGATGAGKSRLSVDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVPH 122

Query: 95  HLLGIADP-NSNFTAADFRRHASSAINSIVGKHRLPIVAGGSNSFMESLVDDDVEFRY-- 154
           HLLG+ +P +   TA +FR  AS+ +  I  + ++PI+AGGSNSF+ +L+    + ++  
Sbjct: 123 HLLGVINPEHGELTAGEFRSAASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFDP 182

Query: 155 ------------RYDCCFLWVDVSLPILHSFVSKRVDRMLDDGFVNEVRNLFDPKENDYV 214
                       RY+CCF+WVDVS  +L+ ++ +RVD M+D G   E+   +DP ++   
Sbjct: 183 FSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGLE 242

Query: 215 R--GIRRAIGVPELDAFLR---AEMDSATDDRARSRILEAAILKMKENTRKLAFRQLQKI 274
              GIR+AIGVPE D + +    E      D  R    + A+  +K NT  LA RQ++KI
Sbjct: 243 TRFGIRKAIGVPEFDGYFKEYPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKKI 302

Query: 275 RRLQNKRGWSVHRIDAT----EVFLQRGDGEYSNQAWEALVAEPSSMIVDQFLYEN 307
             L++  GW + R+DAT     V ++    +   + WE  V EPS  IV + L +N
Sbjct: 303 EMLKD-AGWEIERVDATASFKAVMMKSSSEKKWRENWEEQVLEPSVKIVKRHLVQN 357

[Close]

BLAST of Spg023548 vs. TAIR 10
Match: AT3G19160.1 (ATP/ADP isopentenyltransferases )

HSP 1 Score: 224.6 bits (571), Expect = 1.2e-58
Identity = 121/293 (41.30%), Postives = 180/293 (61.43%), Query Frame = 0


Query: 36  KNKVVFVMGATGTGKSRLAIDLATRFPAEIINSDKIQVYKGLEIVTNKVTEKECCGVPHH 95
           K KVV +MGATG+GKS L+IDLATRF  EI+NSDKIQ Y GL++ TN+++  E CGVPHH
Sbjct: 42  KQKVVVIMGATGSGKSCLSIDLATRFSGEIVNSDKIQFYDGLKVTTNQMSILERCGVPHH 101

Query: 96  LLGIADP-NSNFTAADFRRHASSAINSIVGKHRLPIVAGGSNSFMESLVDD--------- 155
           LLG   P +S  T ++FR  AS +I+ I  +  LPI+AGGSNSF+ +L+ D         
Sbjct: 102 LLGELPPDDSELTTSEFRSLASRSISEITARGNLPIIAGGSNSFIHALLVDRFDPKTYPF 161

Query: 156 ----DVEFRYRYDCCFLWVDVSLPILHSFVSKRVDRMLDDGFVNEVRNLFDPKEND---Y 215
                +    RY+CCFLWVDVS+ +L  ++SKRVD+M++ G   E+   +DP+ +     
Sbjct: 162 SSETSISSGLRYECCFLWVDVSVSVLFEYLSKRVDQMMESGMFEELAGFYDPRYSGSAIR 221

Query: 216 VRGIRRAIGVPELDAFL-----RAEMDSATDDRARSRILEAAILKMKENTRKLAFRQLQK 275
             GI + IG+PE D +        +   +  D+AR    + A+ ++KENT +LA +Q+++
Sbjct: 222 AHGIHKTIGIPEFDRYFSLYPPERKQKMSEWDQARKGAYDEAVQEIKENTWRLAKKQIER 281

Query: 276 IRRLQNKRGWSVHRIDATEVFLQRGDGEYSNQAWEALVAEPSSMIVDQFLYEN 307
           I +L++  GW + R+DAT  F     G  S + W+  V + S  +V +FL ++
Sbjct: 282 IMKLKSS-GWDIQRLDATPSF-----GRSSREIWDNTVLDESIKVVKRFLVKD 328

The following BLAST results are available for this feature:

BLAST of Spg023548 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6575015.1	8.0e-158	86.42	Adenylate isopentenyltransferase 5, chloroplastic, partial [Cucurbita argyrosper...	[more]
KAG7013588.1	4.0e-157	86.11	Adenylate isopentenyltransferase 5, chloroplastic, partial [Cucurbita argyrosper...	[more]
XP_023548308.1	4.0e-157	85.80	adenylate isopentenyltransferase 5, chloroplastic-like [Cucurbita pepo subsp. pe...	[more]
XP_022958992.1	4.8e-155	85.19	adenylate isopentenyltransferase 5, chloroplastic-like [Cucurbita moschata]	[more]
XP_023006220.1	4.5e-153	84.26	adenylate isopentenyltransferase 5, chloroplastic-like [Cucurbita maxima]	[more]

BLAST of Spg023548 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94ID2	8.7e-99	64.48	Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
Q94ID1	3.2e-85	56.31	Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=370...	[more]
Q93WC9	7.2e-85	51.07	Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
Q5GHF7	1.2e-63	44.27	Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1	[more]
Q94ID3	8.0e-60	42.57	Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]

BLAST of Spg023548 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H3C2	2.3e-155	85.19	adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita moschata OX=...	[more]
A0A6J1KZJ7	2.2e-153	84.26	adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita maxima OX=36...	[more]
A0A6J1EWK5	1.4e-147	80.88	adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita moschata OX=...	[more]
A0A6J1KF69	8.1e-140	77.43	adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita maxima OX=36...	[more]
A0A5A7STV9	1.1e-136	80.12	Adenylate isopentenyltransferase 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C...	[more]

BLAST of Spg023548 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G19040.1	6.2e-100	64.48	isopentenyltransferase 5	[more]
AT3G23630.1	2.3e-86	56.31	isopentenyltransferase 7	[more]
AT3G63110.1	5.1e-86	51.07	isopentenyltransferase 3	[more]
AT1G68460.1	5.7e-61	42.57	isopentenyltransferase 1	[more]
AT3G19160.1	1.2e-58	41.30	ATP/ADP isopentenyltransferases	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF01715	IPPT	coord: 151..257 e-value: 1.2E-12 score: 48.1 coord: 72..145 e-value: 3.1E-21 score: 76.2
None	No IPR available	GENE3D	1.10.287.890	Crystal structure of tRNA isopentenylpyrophosphate transferase (bh2366) domain	coord: 164..265 e-value: 5.9E-109 score: 365.4
None	No IPR available	PANTHER	PTHR11088:SF74	ADENYLATE ISOPENTENYLTRANSFERASE 5, CHLOROPLASTIC	coord: 27..308
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 36..303 e-value: 5.9E-109 score: 365.4
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 36..198
IPR039657	Dimethylallyltransferase	PANTHER	PTHR11088	TRNA DIMETHYLALLYLTRANSFERASE	coord: 27..308

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg023548.1	Spg023548.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009691	cytokinin biosynthetic process
biological_process	GO:0006400	tRNA modification
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0052381	tRNA dimethylallyltransferase activity