Spg023502 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg023502
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionubiquitin-conjugating enzyme E2 34-like
Locationscaffold13: 852679 .. 858913 (+)
RNA-Seq ExpressionSpg023502
SyntenySpg023502
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCGCTAGCCGCCTCGGTTTCGCCTGGTTTGTCCCGAAACGCCTCCGAATTCCTAAAAACCCTAGGAGCACGAGCAGGTATTTATACTCCTCTTCGCCACCGAAGAAGGGGACCCGAACTCAATTCTCGACTCTCTTCCCTTGCTCTCTAGCTCTCCCATCCGATTTCTGACTTAAGCATCGGAGGCGGTGTGGCTAGCACCACACCGGTGTGCAGGTTTTCTCTTTTGCAGGCCACGTCTTCCCCCTCATCTACAAATTTACCGTTGGTGGCACGTGAAGGTCAGGCGAGTTTCCTCTGACCAAAATCGACCATCAACAGTTGGCGCCGTCTGTGGGGAAGAAAGCTTGCCAAATCTGCATATCGGTCATTCCATGAGTAAGAGGATGGAGAAGGGAAATCAAGACATAAACACAGAAAATTCGGATGGTGACCGCCACCAGCGGAGGTCACGGGCTGAATCTCCTCGTCCTCGGTCTCCACTGCCCTCATCCCGAGAGAAGCAAGCTAATCTGAAATTTGCTGCTCTCGAAAACAAAGTAAGTGCGATGGATCACAATTTATCCAGGATACTTCATATCTTGGATAAACCTGGACCTAGCACTAAAACCCCTGATGAGAGGTTGGTTAGGGATCCGAGGAAGGGGAAGGAGCCAATCGAGTACACTGCAGAATCGGAGACGAGATCGAAGGGAAAGAAGACTAGCAGCATGACCAGCAAGATCAGGGGGCTCAAGCCTACTGATCGCACAATTTTGAGGAGTCCAGAGTCAAGTACACTTAGAGTCTATAATAGGTTTATCTCTCTTAACTAGCATAAAGCAAAGATCATTCTTTATCTTGATCTAATGCCTAACTCAAAGTTTCCATCTAACCCTTTCACAAATAAATCTTGTTTCCAAAGGATCTTTCACCAATATAGCTTTAAGAAAGCGCCTTGTTTAAAAATAATGATCTCTCAACACCTGGTAATTTAAACTAGGCCAATTTTGCAACAATCTTTCAACTTTGTCTGGCAAACATGATTTGATTAAAAAGCTTGTCATTATCGTTTGAGTCATGGTCAGATTGACATTTATAATTTCCTTATCGAGAGCGGTATAAATATAACTAATTTATTATGTTTTATTAGATACAATTATTTAAGCTTGATTAAAAAAAAAAAAAAAAACCATTACTCAAGTTTAGAGTTTATAATGTGAAATAAGAAAAAAGAAACTGACAATATAATCTAGGTTAAAAATCACTTTTGGTCCATAAACTTTCATGAAAGTAACAATTTGGTCTCTGAACTTTGATTTGTAACGATTTAGTCCATGTACTTTCAATTTCGTAACAATTTAGTTCTTGAACTTTAATAAGTAACAATTTAGTCCCTATACCTTCAAATTTGTAACAATTTAGACCCTACACTTAAATTTGTAACAATTTAATCCTTATCGCAAAAAAAAATCATCAAAATTAAATGTCGATTTTTATTATTTAATGATTAAACTTTGTAGCTTATTAATATATTGGGTCCCAAATTAGTTTACTAATCTATCTAGGTAAGAAATTTCATTAAATCTTTACATTAATTTTTCACAATAGAGACTAAATCGTTACAAATTTGAAAGTACAAATACTAAATTGTTACTTATTAAAATTTAGGGACTAAATCGTTACAAAATTGAAAGTTTAAGAACTAAATCGTTACAAAATTGAAAGTACAGAGACTAAATTGTTATAAATTAAAGTTCAAGAACTAAATTGTTACTTTTATGAAAGTTAAGGGACCAAAAATGAGTTTTAACCTATAATCTATAATTTAATAATCAATTATTAGAGAGAAAAACTTGGGTGTGAATTTACGAAAAAGCCCAGGAAGATAACTGATTTCAAGGGGGCGGATGTGGCTTGGCGAGGGAGTCAACAAACTTCGGGGTGCGGCTGGGGCTGGCTGTATTCTTCCAATTCCATTTCGCGTTCTTACCAAATTTTAATCGCTTCTGCGACTCGCTCCTGTCGCCAAACGCAGACCCATCTTCCTCTGTTAATTAATTCGACGACTGTCAATTTTCCCACCTGATCCACTGCCACCGGACAAATTTCCTCTTCTCAATCCAACGAAAACCCTAGTCCTCAATTTCAGGTAATTCCCACTTCATTTCACTCTCTGATTCATTCCCAGTTTCCCCTTTTCTTTGTCTCGACCAGTGGGGTTCCGATTTCTGTTCGGTTTTCCAATCCGATGCTGCCGGCAGGTTCCTCTATTCACTTCATTTTCTTTTTCTCATTTCAATTCGTCCGTTTATTGTTCGTGGGCGATCCTGATCTAAACTTTCGGGGGTTGGATTAGAGATTGATCACGGATTCAATTTTGAAGAACGAAAGATTGAAATTTGAGAGATTTTATTTTTTTTGTTTGCAGTTTCTCAATTACGACAAAATTGCCGTACTTGCTTTTAATTGCATTATTCGTTTTATTATTGTTATTACTATTTGGCGGATGAGTGATTATAGATACTGTTCTATATTCCTATTCCGATGATTTCTTATTGATTTTGGTGCACTTAAGAAGAATTCTGCTGCGCTTGACAGTATTTAGACTATCTTGGATCCGTTTGCTATGTAGTGGAGAGGGAAATTCTAATTCTTTTTTTGTACTCAGTTAACTACTCTCAACCGCAAATTACGTATTAACACTTCAAGTATTGCGCTGCTGTCTTGTATGAAATTTTTAATGGAAAAGACAAATGATGTGTTAAATATGAAGGATTAACAGCAGAGGGCCTTGCGAGATGCAACAGTAAAAGGTGACTTCAGTAACAAAGAATTACTAGCAATTGAATCTGTTGGTGACCAGGCATTGCTTGTTCCCGCGGTTGAGGCAGCTTCCTTTGTATATTTCTTATTGAAAGTGTTGTTTCTCATCTGTATTGTATATATGATTTATATTGTCTTTATGGTTTCTGTTATAGAAATTTGCTGCATATCATTTTCTTCAGCTAAAATCCCTTCACATCGTATGCTTTCTATTTATAAAATGTATCTTGTCTCTAGAGTTTTTTAAACTATGACCGCTTCTGTACATGGGAAAATTATCATTTACGATGTTCATTAGTTCTTTGCCATAATTCAATATTTTTTCCCTCTGTTTATGTCACCTCACAGGCATTGTATCTGAGGTTGGAGAAAGAATAATAGCAGTTGATTTTCTTTGTCGATAGCTTTGTGGAATGGCAGATAAATCATGTATCAAGAGGCTTCAGAAAGAGTATAGAGCTCTTTGTAAAGTATGTGACTCTGTTTTTGTTTTTTGTTTTTTTCTTTTGTCTTTGAATGATACTGTTAAAATGGATGGATTGGATAAGTAATCTATTGTCCAATCTTGACAATGTAACGTTAGATTGGTGTTTGTCTCATGGCTGATTTCACAAGTGAAATTTTATTTTATTATTTGAGAAACTGAATGTTCTAACTCTTGCTTTGCATTCTTCATGCTGAAATGCGGACATATACTTTTGTATTTGTCCCCCAATTGGTGCTGACTCTGCTATTTGTTAATTTTTAGGAGCCTGTTTCACACATTGTTGCTCGGCCTTCTCCCAGTGACATTCTTGAGTGGCGTAAGTTTGTTCCTATTGATATTGTTTCAGTATTAGTCATGGAGCTCCTAAATTTTGCGTGCATGTAGGAAACAATGGATTTTTGAAGTTGACTTCAGTCTCGTTTTTAACTGATGCAGACTGTAAATGACATGTATTATAATTTTGTGTGTGGAAAGATTGAGAAATAACTGCTAGGACATTGTCATGTTGAATAATGGTGAAACTGTGGGAGTTCTTGGCTGACTGGAAAATATGAGGTCTACAGTCTATTCATTGCTTAGAATAGCACGTAGTGTTAGTAATATGTTGGGACTTGGAAGTCTGTGAAGCATTGCTTGCTTGTTTTATGCCATAATTAGATTCTTATTTTTCTTAATGTTTTGCATCTCTTAAGTTCCAACTACTATTGATGTGATTATTTCTCTCTTATTTGTCATGAAAATATGTGTTTTCTTTCGGCCTATCATTTAGACACTTTAGTTTGCATATTAAATGTTGTCCCATTTTCTCATAAAAAAAAATTGTCCCATTTCAGATTATGTTCTGGAGGGAAGTGAGGGGACTCCTTTTGCAGGTGAGTTTGTTTTTCTTTGTCCCCTTGTATTACACACTGGACACTTCTCTTCTCTTCTCAATTCTTGGTTTCCTTTCATGATTTTCTTCTTTAAGATTACTCTAATTTTGCTCTTTGATTTAAACTTTGATACCAACTTCTTTTTTCTTAGTATTATTATTATTTTTATGGAAATCATTTTAAATCTTTGTTAGGTGGGTACTACCATGGAAAGATTAAATTTCCCCCAGAGTATCCATACAAACCTCCGGGAATTAGGTATAAGCATCTTGGATATGTTTAATAATCTGGAAAATATTTGGGAAGAGATTTTTATAAAACATTACTTAGCTTTCTTATCCACTCATTTTTACTGTGATTTCTGATAATACTACGATTCCTTGTAGCATGATCACCCCAAATGGTCGGTTCATGACACAGAAGAAAATTTGCTTATCTATGAGTGACTGTAAGTTTTTGGATTTTAAAGATGATCATGCCTGCTTGTCTGTAGAAATGTTTCTAAATGACATGATCTGTCTGCAGTTCACCCGGAAAGTTGGAATCCTATGTGGTCTGTGTCTAGGTGAGACATAATTCTTCTCATTTTTCTCCCTGAGGTGTTACAATTTCTTTGTAGTACCCGTGTTTTCTTCTTCTGTTAAAATTTTCTTGAAGTTAACAAATGGTTGCTGTGTATGTGACTAACTTTTCTCTTGGTGCTTTTCAACCTTTGCTTTCTAACACTTGCAGCATTCTAACCGGGCTTCTTTCATTCATGGTGAGTACATTTTGGCTCTTGGCTCATTTTGCATATGGTTGTCTAATAGAAGATGGCTGCTTTTGTGCCTTTATCTGTTCTTATCATCTATAAATTTTGAGAGATGATGTACTTTCTTATGTAGATGGACAACAGTCCTACTACAGGCAGCGTCAATACTACTGCTGCTGAGAAGGAACGCTTGGCAAAGGCTTCTCTTGCATTCAATTGTAAAAAGTAAGTTAATGCGCCCACTGTGAGAATTTTTTGTCCTTGAACGTAGTTCCTCTTCCGGTGAGGTTCCATCTGTCATCCACATTTCCTTATTATGTAGGCTTAGTGAAGTTAATAACCCAGCCCATAATAATTACTGGCAATGTGGATGGCTCATCTCTATCTTTAAAAAACCATGATCTCATATTGCTTTTGGCAGTAGGGTTATTTTGACTTGTTTTCATGGAAACACGGATTCTAGAGACGTGCAACAATGATTATTCTGCTTTAGGCCGTAAAACTTGGGCGGCCTTGAGTGTGTAAATTGCAAGTTATCTTTCTTGAATTTAAAGTTCACACTTACTTTATTGGCATGTCCTTTGCGAAACCACTGTTTATTCTTTTTCTATGTCAACCCACTCATCATCAGTTTATTTTTTTAACTGTACGACTGCAATGCTAATCCATTGCGAAAAAAAAAAAAAAACTTTTATTCAACAGCCCAACATTTAGAAAACTGTTTCCCGAATACGTGGAGAAATACAATCAGCAGCAGCAATCGGAGCAGCTCGTTGCAGAACAGGCTTCAGCAAAGCCATCAGAGGAAGGCGAAGCCAGGCAAATAATGGAGAAGGTCAAGTCCACGGAAGAGAACATCAACAAAGCAGATAGAGAAGACATGAAAAGAAATCGGAAGCAGTCGTTCCCTACATGGATGATGTTGCTACTCTTCTCGATCTTCAGCGTCTTAATGGCATTGCCTCTCCTCCAACCATGAAAGTGCAAGATGGAAGGTCGCATAGGTAATGATAGACAGAGACTAGTTCGTGCAAGTGAAGAATGCTCATATTTTTGCATGTATTAACATAGTGAGTGTCTGTATAGATGGTCTGGTGTCTGTGATGTGGCTTTCATCTTGCTGAGAGTCTGTTATGTTTGGAATAGTTGCTTGAAAACCAAACCTCTTTGTTTGTCTATATATGTTTTTTAAGTACTATTAGCTGAGTGTGACACTGGACAGGGCTCCACTGCAGGATATTGTTTCTTTTTTGCTTTTTAGATTGAAGAGAGTTCCTTGGAGCATCCCAATGTTTCGCCGTGTTTTGCGCTCCACTGC

mRNA sequence

ATGCCGCTAGCCGCCTCGGTTTCGCCTGGTTTGTCCCGAAACGCCTCCGAATTCCTAAAAACCCTAGGAGCACGAGCAGTTGGCGCCGTCTGTGGGGAAGAAAGCTTGCCAAATCTGCATATCGGTCATTCCATGAGTAAGAGGATGGAGAAGGGAAATCAAGACATAAACACAGAAAATTCGGATGGTGACCGCCACCAGCGGAGGTCACGGGCTGAATCTCCTCGTCCTCGGTCTCCACTGCCCTCATCCCGAGAGAAGCAAGCTAATCTGAAATTTGCTGCTCTCGAAAACAAAGTAAGTGCGATGGATCACAATTTATCCAGGATACTTCATATCTTGGATAAACCTGGACCTAGCACTAAAACCCCTGATGAGAGGTTGGTTAGGGATCCGAGGAAGGGGAAGGAGCCAATCGAGTACACTGCAGAATCGGAGACGAGATCGAAGGGAAAGAAGACTAGCAGCATGACCAGCAAGATCAGGGGGCTCAAGCCTACTGATCGCACAATTTTGAGGAGTCCAGAGTCAAGTAATTCCCACTTCATTTCACTCTCTGATTCATTCCCAGTTTCCCCTTTTCTTTGTCTCGACCAGTGGGGTTCCGATTTCTGTTCGGTTTTCCAATCCGATGCTGCCGGCAGGGCCTTGCGAGATGCAACAGTAAAAGGTGACTTCAGTAACAAAGAATTACTAGCAATTGAATCTGTTGGTGACCAGGCATTGCTTGTTCCCGCGGTTGAGGCAGCTTCCTTTCTTTGTGGAATGGCAGATAAATCATGTATCAAGAGGCTTCAGAAAGAGTATAGAGCTCTTTGTAAAGAGCCTGTTTCACACATTGTTGCTCGGCCTTCTCCCAGTGACATTCTTGAGTGGCATTATGTTCTGGAGGGAAGTGAGGGGACTCCTTTTGCAGGTGGGTACTACCATGGAAAGATTAAATTTCCCCCAGAGTATCCATACAAACCTCCGGGAATTAGCATGATCACCCCAAATGGTCGGTTCATGACACAGAAGAAAATTTGCTTATCTATGAGTGACTTTCACCCGGAAAGTTGGAATCCTATGTGGTCTGTGTCTAGCATTCTAACCGGGCTTCTTTCATTCATGATGGACAACAGTCCTACTACAGGCAGCGTCAATACTACTGCTGCTGAGAAGGAACGCTTGGCAAAGGCTTCTCTTGCATTCAATTGTAAAAACCCAACATTTAGAAAACTGTTTCCCGAATACGTGGAGAAATACAATCAGCAGCAGCAATCGGAGCAGCTCGTTGCAGAACAGGCTTCAGCAAAGCCATCAGAGGAAGGCGAAGCCAGGCAAATAATGGAGAAGGTCAAGTCCACGGAAGAGAACATCAACAAAGCAGATAGAGAAGACATGAAAAGAAATCGGAAGCAGTCGTTCCCTACATGGATGATGTTGCTACTCTTCTCGATCTTCAGCGTCTTAATGGCATTGCCTCTCCTCCAACCATGA

Coding sequence (CDS)

ATGCCGCTAGCCGCCTCGGTTTCGCCTGGTTTGTCCCGAAACGCCTCCGAATTCCTAAAAACCCTAGGAGCACGAGCAGTTGGCGCCGTCTGTGGGGAAGAAAGCTTGCCAAATCTGCATATCGGTCATTCCATGAGTAAGAGGATGGAGAAGGGAAATCAAGACATAAACACAGAAAATTCGGATGGTGACCGCCACCAGCGGAGGTCACGGGCTGAATCTCCTCGTCCTCGGTCTCCACTGCCCTCATCCCGAGAGAAGCAAGCTAATCTGAAATTTGCTGCTCTCGAAAACAAAGTAAGTGCGATGGATCACAATTTATCCAGGATACTTCATATCTTGGATAAACCTGGACCTAGCACTAAAACCCCTGATGAGAGGTTGGTTAGGGATCCGAGGAAGGGGAAGGAGCCAATCGAGTACACTGCAGAATCGGAGACGAGATCGAAGGGAAAGAAGACTAGCAGCATGACCAGCAAGATCAGGGGGCTCAAGCCTACTGATCGCACAATTTTGAGGAGTCCAGAGTCAAGTAATTCCCACTTCATTTCACTCTCTGATTCATTCCCAGTTTCCCCTTTTCTTTGTCTCGACCAGTGGGGTTCCGATTTCTGTTCGGTTTTCCAATCCGATGCTGCCGGCAGGGCCTTGCGAGATGCAACAGTAAAAGGTGACTTCAGTAACAAAGAATTACTAGCAATTGAATCTGTTGGTGACCAGGCATTGCTTGTTCCCGCGGTTGAGGCAGCTTCCTTTCTTTGTGGAATGGCAGATAAATCATGTATCAAGAGGCTTCAGAAAGAGTATAGAGCTCTTTGTAAAGAGCCTGTTTCACACATTGTTGCTCGGCCTTCTCCCAGTGACATTCTTGAGTGGCATTATGTTCTGGAGGGAAGTGAGGGGACTCCTTTTGCAGGTGGGTACTACCATGGAAAGATTAAATTTCCCCCAGAGTATCCATACAAACCTCCGGGAATTAGCATGATCACCCCAAATGGTCGGTTCATGACACAGAAGAAAATTTGCTTATCTATGAGTGACTTTCACCCGGAAAGTTGGAATCCTATGTGGTCTGTGTCTAGCATTCTAACCGGGCTTCTTTCATTCATGATGGACAACAGTCCTACTACAGGCAGCGTCAATACTACTGCTGCTGAGAAGGAACGCTTGGCAAAGGCTTCTCTTGCATTCAATTGTAAAAACCCAACATTTAGAAAACTGTTTCCCGAATACGTGGAGAAATACAATCAGCAGCAGCAATCGGAGCAGCTCGTTGCAGAACAGGCTTCAGCAAAGCCATCAGAGGAAGGCGAAGCCAGGCAAATAATGGAGAAGGTCAAGTCCACGGAAGAGAACATCAACAAAGCAGATAGAGAAGACATGAAAAGAAATCGGAAGCAGTCGTTCCCTACATGGATGATGTTGCTACTCTTCTCGATCTTCAGCGTCTTAATGGCATTGCCTCTCCTCCAACCATGA

Protein sequence

MPLAASVSPGLSRNASEFLKTLGARAVGAVCGEESLPNLHIGHSMSKRMEKGNQDINTENSDGDRHQRRSRAESPRPRSPLPSSREKQANLKFAALENKVSAMDHNLSRILHILDKPGPSTKTPDERLVRDPRKGKEPIEYTAESETRSKGKKTSSMTSKIRGLKPTDRTILRSPESSNSHFISLSDSFPVSPFLCLDQWGSDFCSVFQSDAAGRALRDATVKGDFSNKELLAIESVGDQALLVPAVEAASFLCGMADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGGYYHGKIKFPPEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPTTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVEKYNQQQQSEQLVAEQASAKPSEEGEARQIMEKVKSTEENINKADREDMKRNRKQSFPTWMMLLLFSIFSVLMALPLLQP
Homology
BLAST of Spg023502 vs. NCBI nr
Match: XP_038895346.1 (ubiquitin-conjugating enzyme E2 34-like [Benincasa hispida] >XP_038895351.1 ubiquitin-conjugating enzyme E2 34-like [Benincasa hispida] >XP_038895357.1 ubiquitin-conjugating enzyme E2 34-like [Benincasa hispida] >XP_038895365.1 ubiquitin-conjugating enzyme E2 34-like [Benincasa hispida])

HSP 1 Score: 452.2 bits (1162), Expect = 5.7e-123
Identity = 225/237 (94.94%), Postives = 230/237 (97.05%), Query Frame = 0

Query: 256 MADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGGYYHGKIKF 315
           MADK+CIKRLQKEYRALCKEPVSHIVARP+PSDILEWHYVLEGSEGTPFAGG+YHGKIKF
Sbjct: 1   MADKACIKRLQKEYRALCKEPVSHIVARPAPSDILEWHYVLEGSEGTPFAGGFYHGKIKF 60

Query: 316 PPEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSP 375
           PPEYP+KPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSP
Sbjct: 61  PPEYPFKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSP 120

Query: 376 TTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVEKYNQQQQSEQLVAEQASAKPSE 435
           TTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVEKYNQQQ+SEQLVAEQAS K  E
Sbjct: 121 TTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVEKYNQQQKSEQLVAEQASPKSPE 180

Query: 436 EGEARQIMEKVKSTEENINKADREDMKRNRKQSFPTWMMLLLFSIFSVLMALPLLQP 493
           EGE R IMEKVKS EENINKADREDMKRNRKQSFPTWMMLLLFSIF VLMALPLLQP
Sbjct: 181 EGEGRHIMEKVKSAEENINKADREDMKRNRKQSFPTWMMLLLFSIFGVLMALPLLQP 237

BLAST of Spg023502 vs. NCBI nr
Match: XP_023527881.1 (ubiquitin-conjugating enzyme E2 34-like [Cucurbita pepo subsp. pepo] >XP_023527882.1 ubiquitin-conjugating enzyme E2 34-like [Cucurbita pepo subsp. pepo] >KAG6581795.1 Ubiquitin-conjugating enzyme E2 34, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018246.1 Ubiquitin-conjugating enzyme E2 34 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 449.9 bits (1156), Expect = 2.8e-122
Identity = 228/240 (95.00%), Postives = 233/240 (97.08%), Query Frame = 0

Query: 256 MADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGGYYHGKIKF 315
           MADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGG+YHGKIKF
Sbjct: 1   MADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGGFYHGKIKF 60

Query: 316 PPEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSP 375
           PPEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSP
Sbjct: 61  PPEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSP 120

Query: 376 TTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVEKYNQQQQSEQLVAEQASAKPSE 435
           TTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVEKYN+Q QSEQLVAEQAS+KPSE
Sbjct: 121 TTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVEKYNEQHQSEQLVAEQASSKPSE 180

Query: 436 EGEARQIMEKVKS-TEENINK--ADREDMKRNRKQSFPTWMMLLLFSIFSVLMALPLLQP 493
           EG+AR IMEK+KS  EENINK  ADREDMKRNRKQSFPTWMMLLLFSIF VLMALPLLQP
Sbjct: 181 EGKARHIMEKIKSREEENINKAAADREDMKRNRKQSFPTWMMLLLFSIFGVLMALPLLQP 240

BLAST of Spg023502 vs. NCBI nr
Match: XP_022955894.1 (ubiquitin-conjugating enzyme E2 34-like [Cucurbita moschata] >XP_022955896.1 ubiquitin-conjugating enzyme E2 34-like [Cucurbita moschata])

HSP 1 Score: 448.7 bits (1153), Expect = 6.3e-122
Identity = 227/240 (94.58%), Postives = 233/240 (97.08%), Query Frame = 0

Query: 256 MADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGGYYHGKIKF 315
           MADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGG+YHGKIKF
Sbjct: 1   MADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGGFYHGKIKF 60

Query: 316 PPEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSP 375
           PPEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSP
Sbjct: 61  PPEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSP 120

Query: 376 TTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVEKYNQQQQSEQLVAEQASAKPSE 435
           TTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVEKYN+Q QSEQLVAEQAS++PSE
Sbjct: 121 TTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVEKYNEQHQSEQLVAEQASSRPSE 180

Query: 436 EGEARQIMEKVKS-TEENINK--ADREDMKRNRKQSFPTWMMLLLFSIFSVLMALPLLQP 493
           EG+AR IMEK+KS  EENINK  ADREDMKRNRKQSFPTWMMLLLFSIF VLMALPLLQP
Sbjct: 181 EGKARHIMEKIKSREEENINKAAADREDMKRNRKQSFPTWMMLLLFSIFGVLMALPLLQP 240

BLAST of Spg023502 vs. NCBI nr
Match: XP_022998119.1 (ubiquitin-conjugating enzyme E2 34-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 447.6 bits (1150), Expect = 1.4e-121
Identity = 225/243 (92.59%), Postives = 231/243 (95.06%), Query Frame = 0

Query: 251 SFLCGMADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGGYYH 310
           S  CGMADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGGYYH
Sbjct: 39  SIACGMADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGGYYH 98

Query: 311 GKIKFPPEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFM 370
           GKIKFPPEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFM
Sbjct: 99  GKIKFPPEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFM 158

Query: 371 MDNSPTTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVEKYNQQQQSEQLVAEQAS 430
           MDNSPTTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVE +NQQQQSEQLVAEQ S
Sbjct: 159 MDNSPTTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVENFNQQQQSEQLVAEQVS 218

Query: 431 AKPSEEGEARQIMEKVKSTEENINK-ADREDMKRNRKQSFPTWMMLLLFSIFSVLMALPL 490
            KPS EGEAR+++EKVKS E NINK AD + MKRNRKQSFPTW+MLLLFSIF VLMALPL
Sbjct: 219 PKPSVEGEARKLIEKVKSMEGNINKAADGDHMKRNRKQSFPTWLMLLLFSIFGVLMALPL 278

Query: 491 LQP 493
           LQP
Sbjct: 279 LQP 281

BLAST of Spg023502 vs. NCBI nr
Match: XP_022998115.1 (ubiquitin-conjugating enzyme E2 34-like isoform X1 [Cucurbita maxima] >XP_022998116.1 ubiquitin-conjugating enzyme E2 34-like isoform X1 [Cucurbita maxima] >XP_022998118.1 ubiquitin-conjugating enzyme E2 34-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 447.6 bits (1150), Expect = 1.4e-121
Identity = 225/243 (92.59%), Postives = 231/243 (95.06%), Query Frame = 0

Query: 251 SFLCGMADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGGYYH 310
           S  CGMADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGGYYH
Sbjct: 41  SIACGMADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGGYYH 100

Query: 311 GKIKFPPEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFM 370
           GKIKFPPEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFM
Sbjct: 101 GKIKFPPEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFM 160

Query: 371 MDNSPTTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVEKYNQQQQSEQLVAEQAS 430
           MDNSPTTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVE +NQQQQSEQLVAEQ S
Sbjct: 161 MDNSPTTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVENFNQQQQSEQLVAEQVS 220

Query: 431 AKPSEEGEARQIMEKVKSTEENINK-ADREDMKRNRKQSFPTWMMLLLFSIFSVLMALPL 490
            KPS EGEAR+++EKVKS E NINK AD + MKRNRKQSFPTW+MLLLFSIF VLMALPL
Sbjct: 221 PKPSVEGEARKLIEKVKSMEGNINKAADGDHMKRNRKQSFPTWLMLLLFSIFGVLMALPL 280

Query: 491 LQP 493
           LQP
Sbjct: 281 LQP 283

BLAST of Spg023502 vs. ExPASy Swiss-Prot
Match: Q9SHI7 (Ubiquitin-conjugating enzyme E2 34 OS=Arabidopsis thaliana OX=3702 GN=UBC34 PE=2 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 9.5e-97
Identity = 172/236 (72.88%), Postives = 199/236 (84.32%), Query Frame = 0

Query: 256 MADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGGYYHGKIKF 315
           MA+K+CIKRLQKEYRALCKEPVSH+VARPSP+DILEWHYVLEGSEGTPFAGG+Y+GKIKF
Sbjct: 1   MAEKACIKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKF 60

Query: 316 PPEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSP 375
           PPEYPYKPPGI+M TPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMD SP
Sbjct: 61  PPEYPYKPPGITMTTPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDTSP 120

Query: 376 TTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVEKYNQQQQSEQLVAEQASAKPSE 435
           TTGSVNTT  EK+RLAK+SLAFNCK P FRKLFPEYVEKYNQQQ +EQ   +  + +  +
Sbjct: 121 TTGSVNTTVIEKQRLAKSSLAFNCKTPAFRKLFPEYVEKYNQQQLAEQATTQLTTPESPQ 180

Query: 436 EGEARQIMEKVKSTEENINKADREDMKRNRKQSFPTWMMLLLFSIFSVLMALPLLQ 492
           + + +   EK     +  ++   ++ K+N KQ  P W++LLL S+F V+MALPLLQ
Sbjct: 181 KSDTKVESEKTIDPTKGDSEGGLKERKKNNKQGLPAWIILLLVSVFGVVMALPLLQ 236

BLAST of Spg023502 vs. ExPASy Swiss-Prot
Match: Q9FK29 (Probable ubiquitin-conjugating enzyme E2 33 OS=Arabidopsis thaliana OX=3702 GN=UBC33 PE=2 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 6.8e-95
Identity = 174/242 (71.90%), Postives = 206/242 (85.12%), Query Frame = 0

Query: 256 MADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGGYYHGKIKF 315
           MA+K+CIKRLQKEYRALCKEPVSH+VARPSP+DILEWHYVLEGSEGTPFAGG+Y+GKIKF
Sbjct: 1   MAEKACIKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKF 60

Query: 316 PPEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSP 375
           PPEYPYKPPGI+M TPNGRF+TQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSP
Sbjct: 61  PPEYPYKPPGITMTTPNGRFVTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSP 120

Query: 376 TTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVEKYNQQQQSEQLVAEQASAKPSE 435
           TTGSVNT+ AEK+RLAK+SLAFNCK+ TFRKLFPEYVEKY+QQQ +E+  A Q +     
Sbjct: 121 TTGSVNTSVAEKQRLAKSSLAFNCKSVTFRKLFPEYVEKYSQQQVAEEEAATQQTTTSEN 180

Query: 436 EGEARQIMEKVKS------TEENINKADREDMKRNRKQSFPTWMMLLLFSIFSVLMALPL 492
           +   ++   KV+S       +E+I +   ++ +RN+K++ P W++LLL SI  V+MALPL
Sbjct: 181 QDFPQKDNAKVESEKSVGLKKESIQEVGLKERRRNKKEALPGWIVLLLVSIVGVVMALPL 240

BLAST of Spg023502 vs. ExPASy Swiss-Prot
Match: Q2TA03 (Ubiquitin-conjugating enzyme E2 J2 OS=Bos taurus OX=9913 GN=UBE2J2 PE=2 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 1.3e-50
Identity = 93/177 (52.54%), Postives = 129/177 (72.88%), Query Frame = 0

Query: 257 ADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGGYYHGKIKFP 316
           A  +  +RL+++Y  + K+PV +I A P PS+ILEWHYV+ G E TP+ GGYYHGK+ FP
Sbjct: 9   APTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEMTPYEGGYYHGKLIFP 68

Query: 317 PEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPT 376
            E+P+KPP I MITPNGRF    ++CLS++DFHP++WNP WSVS+ILTGLLSFM++  PT
Sbjct: 69  REFPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPT 128

Query: 377 TGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVEKYNQQQQSEQLVAEQASAKP 434
            GS+ T+   K +LA  SL FN K+  F +LFPE VE+  Q+Q+++  ++ +  A P
Sbjct: 129 LGSIETSDFTKRQLAAQSLVFNLKDKVFCELFPEVVEEIKQKQKAQDELSSRPQALP 185

BLAST of Spg023502 vs. ExPASy Swiss-Prot
Match: Q8N2K1 (Ubiquitin-conjugating enzyme E2 J2 OS=Homo sapiens OX=9606 GN=UBE2J2 PE=1 SV=3)

HSP 1 Score: 201.8 bits (512), Expect = 1.8e-50
Identity = 93/177 (52.54%), Postives = 129/177 (72.88%), Query Frame = 0

Query: 257 ADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGGYYHGKIKFP 316
           A  +  +RL+++Y  + K+PV +I A P PS+ILEWHYV+ G E TP+ GGYYHGK+ FP
Sbjct: 9   APTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEMTPYEGGYYHGKLIFP 68

Query: 317 PEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPT 376
            E+P+KPP I MITPNGRF    ++CLS++DFHP++WNP WSVS+ILTGLLSFM++  PT
Sbjct: 69  REFPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPT 128

Query: 377 TGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVEKYNQQQQSEQLVAEQASAKP 434
            GS+ T+   K +LA  SLAFN K+  F +LFPE VE+  Q+Q+++  ++ +    P
Sbjct: 129 LGSIETSDFTKRQLAVQSLAFNLKDKVFCELFPEVVEEIKQKQKAQDELSSRPQTLP 185

BLAST of Spg023502 vs. ExPASy Swiss-Prot
Match: Q6P073 (Ubiquitin-conjugating enzyme E2 J2 OS=Mus musculus OX=10090 GN=Ube2j2 PE=1 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 5.1e-50
Identity = 92/177 (51.98%), Postives = 129/177 (72.88%), Query Frame = 0

Query: 257 ADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGGYYHGKIKFP 316
           A  +  +RL+++Y  + K+PV +I A P PS+ILEWHYV+ G E TP+ GGYYHGK+ FP
Sbjct: 9   APTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEMTPYEGGYYHGKLIFP 68

Query: 317 PEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSPT 376
            E+P+KPP I MITPNGRF    ++CLS++DFHP++WNP WSVS+ILTGLLSFM++  PT
Sbjct: 69  REFPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPT 128

Query: 377 TGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVEKYNQQQQSEQLVAEQASAKP 434
            GS+ T+   K++LA  SL FN K+  F +LFPE VE+  Q+Q+++  ++ +    P
Sbjct: 129 LGSIETSDFTKKQLAAQSLVFNLKDKVFCELFPEVVEEIKQKQKAQDELSNRPQNLP 185

BLAST of Spg023502 vs. ExPASy TrEMBL
Match: A0A6J1GV41 (ubiquitin-conjugating enzyme E2 34-like OS=Cucurbita moschata OX=3662 GN=LOC111457743 PE=4 SV=1)

HSP 1 Score: 448.7 bits (1153), Expect = 3.0e-122
Identity = 227/240 (94.58%), Postives = 233/240 (97.08%), Query Frame = 0

Query: 256 MADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGGYYHGKIKF 315
           MADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGG+YHGKIKF
Sbjct: 1   MADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGGFYHGKIKF 60

Query: 316 PPEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSP 375
           PPEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSP
Sbjct: 61  PPEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSP 120

Query: 376 TTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVEKYNQQQQSEQLVAEQASAKPSE 435
           TTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVEKYN+Q QSEQLVAEQAS++PSE
Sbjct: 121 TTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVEKYNEQHQSEQLVAEQASSRPSE 180

Query: 436 EGEARQIMEKVKS-TEENINK--ADREDMKRNRKQSFPTWMMLLLFSIFSVLMALPLLQP 493
           EG+AR IMEK+KS  EENINK  ADREDMKRNRKQSFPTWMMLLLFSIF VLMALPLLQP
Sbjct: 181 EGKARHIMEKIKSREEENINKAAADREDMKRNRKQSFPTWMMLLLFSIFGVLMALPLLQP 240

BLAST of Spg023502 vs. ExPASy TrEMBL
Match: A0A6J1K9D1 (ubiquitin-conjugating enzyme E2 34-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492862 PE=4 SV=1)

HSP 1 Score: 447.6 bits (1150), Expect = 6.8e-122
Identity = 225/243 (92.59%), Postives = 231/243 (95.06%), Query Frame = 0

Query: 251 SFLCGMADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGGYYH 310
           S  CGMADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGGYYH
Sbjct: 41  SIACGMADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGGYYH 100

Query: 311 GKIKFPPEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFM 370
           GKIKFPPEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFM
Sbjct: 101 GKIKFPPEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFM 160

Query: 371 MDNSPTTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVEKYNQQQQSEQLVAEQAS 430
           MDNSPTTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVE +NQQQQSEQLVAEQ S
Sbjct: 161 MDNSPTTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVENFNQQQQSEQLVAEQVS 220

Query: 431 AKPSEEGEARQIMEKVKSTEENINK-ADREDMKRNRKQSFPTWMMLLLFSIFSVLMALPL 490
            KPS EGEAR+++EKVKS E NINK AD + MKRNRKQSFPTW+MLLLFSIF VLMALPL
Sbjct: 221 PKPSVEGEARKLIEKVKSMEGNINKAADGDHMKRNRKQSFPTWLMLLLFSIFGVLMALPL 280

Query: 491 LQP 493
           LQP
Sbjct: 281 LQP 283

BLAST of Spg023502 vs. ExPASy TrEMBL
Match: A0A6J1KDH1 (ubiquitin-conjugating enzyme E2 34-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492862 PE=4 SV=1)

HSP 1 Score: 447.6 bits (1150), Expect = 6.8e-122
Identity = 225/243 (92.59%), Postives = 231/243 (95.06%), Query Frame = 0

Query: 251 SFLCGMADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGGYYH 310
           S  CGMADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGGYYH
Sbjct: 39  SIACGMADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGGYYH 98

Query: 311 GKIKFPPEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFM 370
           GKIKFPPEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFM
Sbjct: 99  GKIKFPPEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFM 158

Query: 371 MDNSPTTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVEKYNQQQQSEQLVAEQAS 430
           MDNSPTTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVE +NQQQQSEQLVAEQ S
Sbjct: 159 MDNSPTTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVENFNQQQQSEQLVAEQVS 218

Query: 431 AKPSEEGEARQIMEKVKSTEENINK-ADREDMKRNRKQSFPTWMMLLLFSIFSVLMALPL 490
            KPS EGEAR+++EKVKS E NINK AD + MKRNRKQSFPTW+MLLLFSIF VLMALPL
Sbjct: 219 PKPSVEGEARKLIEKVKSMEGNINKAADGDHMKRNRKQSFPTWLMLLLFSIFGVLMALPL 278

Query: 491 LQP 493
           LQP
Sbjct: 279 LQP 281

BLAST of Spg023502 vs. ExPASy TrEMBL
Match: A0A6J1IXB2 (ubiquitin-conjugating enzyme E2 34-like OS=Cucurbita maxima OX=3661 GN=LOC111480001 PE=4 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 1.2e-121
Identity = 226/240 (94.17%), Postives = 231/240 (96.25%), Query Frame = 0

Query: 256 MADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGGYYHGKIKF 315
           MADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGG+YHGKIKF
Sbjct: 1   MADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGGFYHGKIKF 60

Query: 316 PPEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSP 375
           PPEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSP
Sbjct: 61  PPEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSP 120

Query: 376 TTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVEKYNQQQQSEQLVAEQASAKPSE 435
           TTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVEKYN+Q QSEQL AEQAS+KPSE
Sbjct: 121 TTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVEKYNEQHQSEQLAAEQASSKPSE 180

Query: 436 EGEARQIMEKVKS-TEENINK--ADREDMKRNRKQSFPTWMMLLLFSIFSVLMALPLLQP 493
           EG+ R IMEK+KS  EENINK  ADREDMKRNRKQSFPTWMMLLLFSIF VLMALPLLQP
Sbjct: 181 EGKTRHIMEKIKSREEENINKVAADREDMKRNRKQSFPTWMMLLLFSIFGVLMALPLLQP 240

BLAST of Spg023502 vs. ExPASy TrEMBL
Match: A0A5D3D6L3 (Ubiquitin-conjugating enzyme E2 34-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold522G001210 PE=4 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 2.2e-120
Identity = 218/237 (91.98%), Postives = 229/237 (96.62%), Query Frame = 0

Query: 256 MADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGGYYHGKIKF 315
           MADK+CIKRLQKEYRALCKEPVSHIVARP+PSDILEWHYVLEGSEGTPFAGG+YHGKIKF
Sbjct: 1   MADKACIKRLQKEYRALCKEPVSHIVARPAPSDILEWHYVLEGSEGTPFAGGFYHGKIKF 60

Query: 316 PPEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSP 375
           PPEYP+KPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSP
Sbjct: 61  PPEYPFKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSP 120

Query: 376 TTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVEKYNQQQQSEQLVAEQASAKPSE 435
           TTGSVNTTAAEKERLAK SLAFNCKNPTFRKLFPEYVEKYNQQQQSEQLVAEQAS K SE
Sbjct: 121 TTGSVNTTAAEKERLAKTSLAFNCKNPTFRKLFPEYVEKYNQQQQSEQLVAEQASPKSSE 180

Query: 436 EGEARQIMEKVKSTEENINKADREDMKRNRKQSFPTWMMLLLFSIFSVLMALPLLQP 493
           EGE + IM++V+S EENINKAD EDMK+NRKQSFPTW++LLLFSIF VLMALPLLQP
Sbjct: 181 EGEGKHIMKRVRSMEENINKADGEDMKKNRKQSFPTWLLLLLFSIFGVLMALPLLQP 237

BLAST of Spg023502 vs. TAIR 10
Match: AT1G17280.1 (ubiquitin-conjugating enzyme 34 )

HSP 1 Score: 355.5 bits (911), Expect = 6.7e-98
Identity = 172/236 (72.88%), Postives = 199/236 (84.32%), Query Frame = 0

Query: 256 MADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGGYYHGKIKF 315
           MA+K+CIKRLQKEYRALCKEPVSH+VARPSP+DILEWHYVLEGSEGTPFAGG+Y+GKIKF
Sbjct: 1   MAEKACIKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKF 60

Query: 316 PPEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSP 375
           PPEYPYKPPGI+M TPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMD SP
Sbjct: 61  PPEYPYKPPGITMTTPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDTSP 120

Query: 376 TTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVEKYNQQQQSEQLVAEQASAKPSE 435
           TTGSVNTT  EK+RLAK+SLAFNCK P FRKLFPEYVEKYNQQQ +EQ   +  + +  +
Sbjct: 121 TTGSVNTTVIEKQRLAKSSLAFNCKTPAFRKLFPEYVEKYNQQQLAEQATTQLTTPESPQ 180

Query: 436 EGEARQIMEKVKSTEENINKADREDMKRNRKQSFPTWMMLLLFSIFSVLMALPLLQ 492
           + + +   EK     +  ++   ++ K+N KQ  P W++LLL S+F V+MALPLLQ
Sbjct: 181 KSDTKVESEKTIDPTKGDSEGGLKERKKNNKQGLPAWIILLLVSVFGVVMALPLLQ 236

BLAST of Spg023502 vs. TAIR 10
Match: AT1G17280.2 (ubiquitin-conjugating enzyme 34 )

HSP 1 Score: 355.5 bits (911), Expect = 6.7e-98
Identity = 172/236 (72.88%), Postives = 199/236 (84.32%), Query Frame = 0

Query: 256 MADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGGYYHGKIKF 315
           MA+K+CIKRLQKEYRALCKEPVSH+VARPSP+DILEWHYVLEGSEGTPFAGG+Y+GKIKF
Sbjct: 1   MAEKACIKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKF 60

Query: 316 PPEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSP 375
           PPEYPYKPPGI+M TPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMD SP
Sbjct: 61  PPEYPYKPPGITMTTPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDTSP 120

Query: 376 TTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVEKYNQQQQSEQLVAEQASAKPSE 435
           TTGSVNTT  EK+RLAK+SLAFNCK P FRKLFPEYVEKYNQQQ +EQ   +  + +  +
Sbjct: 121 TTGSVNTTVIEKQRLAKSSLAFNCKTPAFRKLFPEYVEKYNQQQLAEQATTQLTTPESPQ 180

Query: 436 EGEARQIMEKVKSTEENINKADREDMKRNRKQSFPTWMMLLLFSIFSVLMALPLLQ 492
           + + +   EK     +  ++   ++ K+N KQ  P W++LLL S+F V+MALPLLQ
Sbjct: 181 KSDTKVESEKTIDPTKGDSEGGLKERKKNNKQGLPAWIILLLVSVFGVVMALPLLQ 236

BLAST of Spg023502 vs. TAIR 10
Match: AT5G50430.1 (ubiquitin-conjugating enzyme 33 )

HSP 1 Score: 349.4 bits (895), Expect = 4.8e-96
Identity = 174/242 (71.90%), Postives = 206/242 (85.12%), Query Frame = 0

Query: 256 MADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGGYYHGKIKF 315
           MA+K+CIKRLQKEYRALCKEPVSH+VARPSP+DILEWHYVLEGSEGTPFAGG+Y+GKIKF
Sbjct: 1   MAEKACIKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKF 60

Query: 316 PPEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSP 375
           PPEYPYKPPGI+M TPNGRF+TQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSP
Sbjct: 61  PPEYPYKPPGITMTTPNGRFVTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSP 120

Query: 376 TTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVEKYNQQQQSEQLVAEQASAKPSE 435
           TTGSVNT+ AEK+RLAK+SLAFNCK+ TFRKLFPEYVEKY+QQQ +E+  A Q +     
Sbjct: 121 TTGSVNTSVAEKQRLAKSSLAFNCKSVTFRKLFPEYVEKYSQQQVAEEEAATQQTTTSEN 180

Query: 436 EGEARQIMEKVKS------TEENINKADREDMKRNRKQSFPTWMMLLLFSIFSVLMALPL 492
           +   ++   KV+S       +E+I +   ++ +RN+K++ P W++LLL SI  V+MALPL
Sbjct: 181 QDFPQKDNAKVESEKSVGLKKESIQEVGLKERRRNKKEALPGWIVLLLVSIVGVVMALPL 240

BLAST of Spg023502 vs. TAIR 10
Match: AT5G50430.2 (ubiquitin-conjugating enzyme 33 )

HSP 1 Score: 349.4 bits (895), Expect = 4.8e-96
Identity = 174/242 (71.90%), Postives = 206/242 (85.12%), Query Frame = 0

Query: 256 MADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGGYYHGKIKF 315
           MA+K+CIKRLQKEYRALCKEPVSH+VARPSP+DILEWHYVLEGSEGTPFAGG+Y+GKIKF
Sbjct: 1   MAEKACIKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKF 60

Query: 316 PPEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSP 375
           PPEYPYKPPGI+M TPNGRF+TQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSP
Sbjct: 61  PPEYPYKPPGITMTTPNGRFVTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSP 120

Query: 376 TTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVEKYNQQQQSEQLVAEQASAKPSE 435
           TTGSVNT+ AEK+RLAK+SLAFNCK+ TFRKLFPEYVEKY+QQQ +E+  A Q +     
Sbjct: 121 TTGSVNTSVAEKQRLAKSSLAFNCKSVTFRKLFPEYVEKYSQQQVAEEEAATQQTTTSEN 180

Query: 436 EGEARQIMEKVKS------TEENINKADREDMKRNRKQSFPTWMMLLLFSIFSVLMALPL 492
           +   ++   KV+S       +E+I +   ++ +RN+K++ P W++LLL SI  V+MALPL
Sbjct: 181 QDFPQKDNAKVESEKSVGLKKESIQEVGLKERRRNKKEALPGWIVLLLVSIVGVVMALPL 240

BLAST of Spg023502 vs. TAIR 10
Match: AT5G50430.3 (ubiquitin-conjugating enzyme 33 )

HSP 1 Score: 309.7 bits (792), Expect = 4.2e-84
Identity = 160/242 (66.12%), Postives = 192/242 (79.34%), Query Frame = 0

Query: 256 MADKSCIKRLQKEYRALCKEPVSHIVARPSPSDILEWHYVLEGSEGTPFAGGYYHGKIKF 315
           MA+K+CIKRLQKEYRALCKEPVSH+VARPSP+DILEW              G+Y+GKIKF
Sbjct: 1   MAEKACIKRLQKEYRALCKEPVSHVVARPSPNDILEWR-------------GFYYGKIKF 60

Query: 316 PPEYPYKPPGISMITPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSP 375
           PPEYPYKPPGI+M TPNGRF+TQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSP
Sbjct: 61  PPEYPYKPPGITMTTPNGRFVTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDNSP 120

Query: 376 TTGSVNTTAAEKERLAKASLAFNCKNPTFRKLFPEYVEKYNQQQQSEQLVAEQASAKPSE 435
           TTGSVNT+ AEK+RLAK+SLAFNCK+ TFRKLFPEYVEKY+QQQ +E+  A Q +     
Sbjct: 121 TTGSVNTSVAEKQRLAKSSLAFNCKSVTFRKLFPEYVEKYSQQQVAEEEAATQQTTTSEN 180

Query: 436 EGEARQIMEKVKS------TEENINKADREDMKRNRKQSFPTWMMLLLFSIFSVLMALPL 492
           +   ++   KV+S       +E+I +   ++ +RN+K++ P W++LLL SI  V+MALPL
Sbjct: 181 QDFPQKDNAKVESEKSVGLKKESIQEVGLKERRRNKKEALPGWIVLLLVSIVGVVMALPL 229

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038895346.15.7e-12394.94ubiquitin-conjugating enzyme E2 34-like [Benincasa hispida] >XP_038895351.1 ubiq... [more]
XP_023527881.12.8e-12295.00ubiquitin-conjugating enzyme E2 34-like [Cucurbita pepo subsp. pepo] >XP_0235278... [more]
XP_022955894.16.3e-12294.58ubiquitin-conjugating enzyme E2 34-like [Cucurbita moschata] >XP_022955896.1 ubi... [more]
XP_022998119.11.4e-12192.59ubiquitin-conjugating enzyme E2 34-like isoform X2 [Cucurbita maxima][more]
XP_022998115.11.4e-12192.59ubiquitin-conjugating enzyme E2 34-like isoform X1 [Cucurbita maxima] >XP_022998... [more]
Match NameE-valueIdentityDescription
Q9SHI79.5e-9772.88Ubiquitin-conjugating enzyme E2 34 OS=Arabidopsis thaliana OX=3702 GN=UBC34 PE=2... [more]
Q9FK296.8e-9571.90Probable ubiquitin-conjugating enzyme E2 33 OS=Arabidopsis thaliana OX=3702 GN=U... [more]
Q2TA031.3e-5052.54Ubiquitin-conjugating enzyme E2 J2 OS=Bos taurus OX=9913 GN=UBE2J2 PE=2 SV=1[more]
Q8N2K11.8e-5052.54Ubiquitin-conjugating enzyme E2 J2 OS=Homo sapiens OX=9606 GN=UBE2J2 PE=1 SV=3[more]
Q6P0735.1e-5051.98Ubiquitin-conjugating enzyme E2 J2 OS=Mus musculus OX=10090 GN=Ube2j2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1GV413.0e-12294.58ubiquitin-conjugating enzyme E2 34-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1K9D16.8e-12292.59ubiquitin-conjugating enzyme E2 34-like isoform X1 OS=Cucurbita maxima OX=3661 G... [more]
A0A6J1KDH16.8e-12292.59ubiquitin-conjugating enzyme E2 34-like isoform X2 OS=Cucurbita maxima OX=3661 G... [more]
A0A6J1IXB21.2e-12194.17ubiquitin-conjugating enzyme E2 34-like OS=Cucurbita maxima OX=3661 GN=LOC111480... [more]
A0A5D3D6L32.2e-12091.98Ubiquitin-conjugating enzyme E2 34-like OS=Cucumis melo var. makuwa OX=1194695 G... [more]
Match NameE-valueIdentityDescription
AT1G17280.16.7e-9872.88ubiquitin-conjugating enzyme 34 [more]
AT1G17280.26.7e-9872.88ubiquitin-conjugating enzyme 34 [more]
AT5G50430.14.8e-9671.90ubiquitin-conjugating enzyme 33 [more]
AT5G50430.24.8e-9671.90ubiquitin-conjugating enzyme 33 [more]
AT5G50430.34.2e-8466.12ubiquitin-conjugating enzyme 33 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 439..459
NoneNo IPR availableSMARTSM00212ubc_7coord: 263..417
e-value: 1.5E-37
score: 140.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 53..86
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 119..153
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 114..162
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 53..75
NoneNo IPR availablePANTHERPTHR24068UBIQUITIN-CONJUGATING ENZYME E2coord: 258..491
NoneNo IPR availablePANTHERPTHR24068:SF290UBIQUITIN-CONJUGATING ENZYME E2 34-LIKEcoord: 258..491
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 264..386
e-value: 1.8E-19
score: 69.8
IPR000608Ubiquitin-conjugating enzyme E2PROSITEPS50127UBC_2coord: 260..417
score: 48.259987
IPR000608Ubiquitin-conjugating enzyme E2CDDcd00195UBCccoord: 262..412
e-value: 3.21084E-48
score: 161.216
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3D3.10.110.10Ubiquitin Conjugating Enzymecoord: 255..433
e-value: 1.0E-60
score: 206.4
IPR016135Ubiquitin-conjugating enzyme/RWD-likeSUPERFAMILY54495UBC-likecoord: 261..413

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg023502.1Spg023502.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016746 acyltransferase activity