Spg023287 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg023287
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	RNase H domain-containing protein
Location	scaffold13: 7456515 .. 7457130 (-)
RNA-Seq Expression	Spg023287
Synteny	Spg023287

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATCAGAAACTCTTGGATAAAAGAATACTTAGAAGAATTTTCGAAAGCAAACCTGAGAGAAGCTCCAAGAGAAGTTTCGAGCCCTCGCCTTGGTCCAAAATGATAAAAAATGGTCTCCGCCTCCTGAAGGATACTGGAAATTCAATGTTGATGCTGCCTAGTCGCAATTGATCCCAACCACTAGAATTGGTGTAGTTTGCAGAGACTCAAAAGGCGATGCCCTAGGAGCGAAAACCATTTTTCTTGATGCGGACTTTAGGGCTCCTTTGATAGAATTGAAAGCTATCGGAGAAGGAATCAAGCTGGCAAAACTATTGGGTTGCAATAAAATCATAATTGAATCTGACTGTACTCAAGCCATTGACCTTGTATCTAAAAAGTCAGCCCCCTGGGGTGAGTTGGAAGCAGAGCTGGAGACCATATGGTCAGATGTGGCAAGTTTTTCTGATTGTTCCTTTATTCATACTCCTAAGAAGAAGAATTTAGTGGCAGATAAAATAGCTAAAAGAGCGAGAATGTTAAATTCGAGTGATATCTGGGTTTTCTCTATCTCGGATTGGATTGTATCTTTGGTCGATTCGGACTGTTCATCGTTTGTCCAAGTGGCGCCTTAA

mRNA sequence

ATGATCAGAAACTCTTGGATAAAAGAATACTTAGAAGAATTTTCGAAAGCAAACCTGAGAGAAGCTCCAAGAGAAGTTTCGAGCCCTCGCCTTGTTTGCAGAGACTCAAAAGGCGATGCCCTAGGAGCGAAAACCATTTTTCTTGATGCGGACTTTAGGGCTCCTTTGATAGAATTGAAAGCTATCGGAGAAGGAATCAAGCTGGCAAAACTATTGGGTTGCAATAAAATCATAATTGAATCTGACTGTACTCAAGCCATTGACCTTGTATCTAAAAAGTCAGCCCCCTGGGGTGAGTTGGAAGCAGAGCTGGAGACCATATGGTCAGATGTGGCAAGTTTTTCTGATTGTTCCTTTATTCATACTCCTAAGAAGAAGAATTTAGTGGCAGATAAAATAGCTAAAAGAGCGAGAATGTTAAATTCGAGTGATATCTGGGTTTTCTCTATCTCGGATTGGATTGTATCTTTGGTCGATTCGGACTGTTCATCGTTTGTCCAAGTGGCGCCTTAA

Coding sequence (CDS)

ATGATCAGAAACTCTTGGATAAAAGAATACTTAGAAGAATTTTCGAAAGCAAACCTGAGAGAAGCTCCAAGAGAAGTTTCGAGCCCTCGCCTTGTTTGCAGAGACTCAAAAGGCGATGCCCTAGGAGCGAAAACCATTTTTCTTGATGCGGACTTTAGGGCTCCTTTGATAGAATTGAAAGCTATCGGAGAAGGAATCAAGCTGGCAAAACTATTGGGTTGCAATAAAATCATAATTGAATCTGACTGTACTCAAGCCATTGACCTTGTATCTAAAAAGTCAGCCCCCTGGGGTGAGTTGGAAGCAGAGCTGGAGACCATATGGTCAGATGTGGCAAGTTTTTCTGATTGTTCCTTTATTCATACTCCTAAGAAGAAGAATTTAGTGGCAGATAAAATAGCTAAAAGAGCGAGAATGTTAAATTCGAGTGATATCTGGGTTTTCTCTATCTCGGATTGGATTGTATCTTTGGTCGATTCGGACTGTTCATCGTTTGTCCAAGTGGCGCCTTAA

Protein sequence

MIRNSWIKEYLEEFSKANLREAPREVSSPRLVCRDSKGDALGAKTIFLDADFRAPLIELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVSKKSAPWGELEAELETIWSDVASFSDCSFIHTPKKKNLVADKIAKRARMLNSSDIWVFSISDWIVSLVDSDCSSFVQVAP

Homology

[Close]

BLAST of Spg023287 vs. NCBI nr
Match: XP_038902513.1 (uncharacterized protein LOC120089172 [Benincasa hispida])

HSP 1 Score: 75.5 bits (184), Expect = 5.0e-10
Identity = 40/120 (33.33%), Postives = 64/120 (53.33%), Query Frame = 0


Query: 32  VCRDSKGDALGAKTIFLDADFRAPLIELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVS 91
           + RD++G     +   +DA +  PL E   + +G++L   +   KII++SDC+ AIDL  
Sbjct: 84  IIRDNQGSLKVVQISSIDAVYPPPLAEAFVVLQGLRLTSKMNFKKIIVKSDCSGAIDLFL 143

Query: 92  KKSAPWGELEAELETIWSDVASFSDCSFIHTPKKKNLVADKIAKRARMLNSSDIWVFSIS 151
           K       +   LE IW     F   +F + P+  N +AD +AKR R+L  +D+W+ S S
Sbjct: 144 KILISDSSVRVWLEEIWEISIVFYPINFAYIPRNINKLADIVAKRTRILGINDVWMDSYS 203

[Close]

BLAST of Spg023287 vs. NCBI nr
Match: XP_023915006.1 (uncharacterized protein LOC112026546 [Quercus suber])

HSP 1 Score: 74.7 bits (182), Expect = 8.5e-10
Identity = 45/152 (29.61%), Postives = 80/152 (52.63%), Query Frame = 0


Query: 14  FSKANLREAPRE---VSSPRLVCRDSKGDALGAKTIFLDADFRAPLIELKAIGEGIKLAK 73
           F+K N+  A  +    SS  ++ RDS+G ++ A    L + F A + E  A+ +G+ LA 
Sbjct: 355 FTKINVDGAASDDGRPSSTGVIIRDSQGSSIAASCRVLSSPFPADISEALALQDGVMLAL 414

Query: 74  LLGCNKIIIESDCTQAIDLVSKKSAPWGELEAELETIWSDVASFSDCSFIHTPKKKNLVA 133
            LG +K+I ESD    I  +++ +   GE+   ++ I    +SFS C+F H  ++ N  A
Sbjct: 415 ELGLSKVIFESDALSIIQAINEGNV-GGEIGHIMQNIKDLASSFSWCTFQHQKREGNRAA 474

Query: 134 DKIAKRARMLNSSDIWVFSISDWIVSLVDSDC 163
            ++A+ ARM   S +W      ++  ++  +C
Sbjct: 475 HELARAARMTGISQVWKGVSPSFVEHIICEEC 505

[Close]

BLAST of Spg023287 vs. NCBI nr
Match: VAH20608.1 (unnamed protein product [Triticum turgidum subsp. durum])

HSP 1 Score: 73.2 bits (178), Expect = 2.5e-09
Identity = 44/132 (33.33%), Postives = 71/132 (53.79%), Query Frame = 0


Query: 32  VCRDSKGDALGAKTIFLDADFRAPLIELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVS 91
           V RD +GD + A + F+     A   E+ AI  G+ LA  +GCN +I+ESD + A+D+++
Sbjct: 30  VARDDRGDFIAATSCFISHVTSAKSAEVVAIRNGLILAMTIGCNSLILESDSSNAVDIIN 89

Query: 92  KKSAPWGELEAELET--IWSDVASFSDCSFIHTPKKKNLVADKIAKRARMLNSSDIWVFS 151
           ++     E+   +E   + SD   F+    IH  ++ N VAD IAK A    SS +W   
Sbjct: 90  QEDYLGQEVSIYMECRHLGSD---FAKVEVIHCFREANSVADSIAKNALSTRSSMVWDEG 149

Query: 152 ISDWIVSLVDSD 162
           + D+I   + +D
Sbjct: 150 VPDFISLQIVND 158

[Close]

BLAST of Spg023287 vs. NCBI nr
Match: EMS54402.1 (Ketohexokinase [Triticum urartu])

HSP 1 Score: 72.0 bits (175), Expect = 5.5e-09
Identity = 48/132 (36.36%), Postives = 68/132 (51.52%), Query Frame = 0


Query: 32  VCRDSKGDALGAKTIFLDADFRAPLIELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVS 91
           V RD +GD + A + F+         EL AI  GI LA  +GCN I IESDC+ A+D ++
Sbjct: 413 VARDYRGDFITAASWFVLNISGVDSAELTAIRNGIYLATHIGCNNIEIESDCSFAVDSLN 472

Query: 92  KKSAPWGELEAELETIWSDVASFSDCSFIHTPKKKNLVADKIAKRARMLNSSDIWVFSIS 151
           +     G   A +      +  F+  SF H  ++ N VADK+AK +    SS  W   I 
Sbjct: 473 QVDDYLGPEVAIIIEYKQLMMDFASISFRHCYREANQVADKLAKNSFTTRSSSSWDDEIP 532

Query: 152 DWIVSLVDSDCS 164
           D+I  L+ +D S
Sbjct: 533 DFISHLLVNDMS 544

[Close]

BLAST of Spg023287 vs. NCBI nr
Match: VAH65567.1 (unnamed protein product [Triticum turgidum subsp. durum])

HSP 1 Score: 70.9 bits (172), Expect = 1.2e-08
Identity = 46/132 (34.85%), Postives = 67/132 (50.76%), Query Frame = 0


Query: 32  VCRDSKGDALGAKTIFLDADFRAPLIELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVS 91
           V RD +GD + A + F+         EL AI  GI LA  +GCN I IESDC+ A++ ++
Sbjct: 102 VARDYRGDFIAAASWFVPNISGVDSAELTAIRNGIYLATHIGCNNIEIESDCSFAVNSLN 161

Query: 92  KKSAPWGELEAELETIWSDVASFSDCSFIHTPKKKNLVADKIAKRARMLNSSDIWVFSIS 151
           +     G   A +      +  F+  SF H  ++ N VAD++AK      SS  W   I 
Sbjct: 162 QVDDYLGPEVAIITEYKQLMMDFASISFRHCYREANQVADELAKNTFTTRSSSSWDDEIP 221

Query: 152 DWIVSLVDSDCS 164
           D+I  L+ +D S
Sbjct: 222 DFISHLLVNDMS 233

[Close]

BLAST of Spg023287 vs. ExPASy TrEMBL
Match: A0A446K5I3 (RNase H domain-containing protein OS=Triticum turgidum subsp. durum OX=4567 GN=TRITD_1Bv1G175360 PE=4 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 1.2e-09
Identity = 44/132 (33.33%), Postives = 71/132 (53.79%), Query Frame = 0


Query: 32  VCRDSKGDALGAKTIFLDADFRAPLIELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVS 91
           V RD +GD + A + F+     A   E+ AI  G+ LA  +GCN +I+ESD + A+D+++
Sbjct: 30  VARDDRGDFIAATSCFISHVTSAKSAEVVAIRNGLILAMTIGCNSLILESDSSNAVDIIN 89

Query: 92  KKSAPWGELEAELET--IWSDVASFSDCSFIHTPKKKNLVADKIAKRARMLNSSDIWVFS 151
           ++     E+   +E   + SD   F+    IH  ++ N VAD IAK A    SS +W   
Sbjct: 90  QEDYLGQEVSIYMECRHLGSD---FAKVEVIHCFREANSVADSIAKNALSTRSSMVWDEG 149

Query: 152 ISDWIVSLVDSD 162
           + D+I   + +D
Sbjct: 150 VPDFISLQIVND 158

[Close]

BLAST of Spg023287 vs. ExPASy TrEMBL
Match: M8AHN8 (Zinc finger CCCH domain-containing protein 7 OS=Aegilops tauschii OX=37682 GN=F775_04738 PE=4 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 1.6e-09
Identity = 52/155 (33.55%), Postives = 77/155 (49.68%), Query Frame = 0


Query: 9   EYLEEFSKANLREAPREVSSPRLVCRDSKGDALGAKTIFLDADFRAPLIELKAIGEGIKL 68
           E + EF  A L  A      P   CRD +G+ +GA + F+     A   E+ AI  G+ L
Sbjct: 298 ELVGEFPMAEL-VAVASWELPEQFCRDDQGEFIGAASWFVSHVQSAETSEIVAIRNGLYL 357

Query: 69  AKLLGCNKIIIESDCTQAIDLVSKKS--APWGELEAELETIWSDVASFSDCSFIHTPKKK 128
           A  LGCN +I+ESD   AI+  ++     P   + AE +TI  D+A F      H  ++ 
Sbjct: 358 AAKLGCNSVIVESDSLNAIEAYNQDDYLDPDAAVVAEGKTIGEDLAKF---DVAHCFREA 417

Query: 129 NLVADKIAKRARMLNSSDIWVFSISDWIVSLVDSD 162
           N VAD IAK +   +S++ W   I D++   +  D
Sbjct: 418 NGVADSIAKHSLCNSSTEYWEGPIPDFMSHFIVHD 448

[Close]

BLAST of Spg023287 vs. ExPASy TrEMBL
Match: M7Z366 (Ketohexokinase OS=Triticum urartu OX=4572 GN=TRIUR3_11649 PE=3 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 2.7e-09
Identity = 48/132 (36.36%), Postives = 68/132 (51.52%), Query Frame = 0


Query: 32  VCRDSKGDALGAKTIFLDADFRAPLIELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVS 91
           V RD +GD + A + F+         EL AI  GI LA  +GCN I IESDC+ A+D ++
Sbjct: 413 VARDYRGDFITAASWFVLNISGVDSAELTAIRNGIYLATHIGCNNIEIESDCSFAVDSLN 472

Query: 92  KKSAPWGELEAELETIWSDVASFSDCSFIHTPKKKNLVADKIAKRARMLNSSDIWVFSIS 151
           +     G   A +      +  F+  SF H  ++ N VADK+AK +    SS  W   I 
Sbjct: 473 QVDDYLGPEVAIIIEYKQLMMDFASISFRHCYREANQVADKLAKNSFTTRSSSSWDDEIP 532

Query: 152 DWIVSLVDSDCS 164
           D+I  L+ +D S
Sbjct: 533 DFISHLLVNDMS 544

[Close]

BLAST of Spg023287 vs. ExPASy TrEMBL
Match: A0A446NU09 (RNase H domain-containing protein OS=Triticum turgidum subsp. durum OX=4567 GN=TRITD_3Av1G213860 PE=4 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 6.0e-09
Identity = 46/132 (34.85%), Postives = 67/132 (50.76%), Query Frame = 0


Query: 32  VCRDSKGDALGAKTIFLDADFRAPLIELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVS 91
           V RD +GD + A + F+         EL AI  GI LA  +GCN I IESDC+ A++ ++
Sbjct: 102 VARDYRGDFIAAASWFVPNISGVDSAELTAIRNGIYLATHIGCNNIEIESDCSFAVNSLN 161

Query: 92  KKSAPWGELEAELETIWSDVASFSDCSFIHTPKKKNLVADKIAKRARMLNSSDIWVFSIS 151
           +     G   A +      +  F+  SF H  ++ N VAD++AK      SS  W   I 
Sbjct: 162 QVDDYLGPEVAIITEYKQLMMDFASISFRHCYREANQVADELAKNTFTTRSSSSWDDEIP 221

Query: 152 DWIVSLVDSDCS 164
           D+I  L+ +D S
Sbjct: 222 DFISHLLVNDMS 233

[Close]

BLAST of Spg023287 vs. ExPASy TrEMBL
Match: A0A446W4K1 (RNase H domain-containing protein OS=Triticum turgidum subsp. durum OX=4567 GN=TRITD_6Bv1G045260 PE=4 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 1.9e-07
Identity = 38/132 (28.79%), Postives = 65/132 (49.24%), Query Frame = 0


Query: 32  VCRDSKGDALGAKTIFLDADFRAPLIELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVS 91
           + RD +G+ + A   F+     A   E++AI  G  LA+ +GCN +I+ESD   A+    
Sbjct: 62  IARDDRGEFIAAAAWFIPHVITAESAEIQAIRNGAWLAQHIGCNSLILESDNLNAVQAFD 121

Query: 92  KKSAPWGELEAELETIWSDVASFSDCSFIHTPKKKNLVADKIAKRARMLNSSDIWVFSIS 151
           ++    G     +         F+  S++H  ++ N VAD +AK + +  SS +W  S  
Sbjct: 122 QQETYNGNDAVVVMDGKQTCTDFAAVSYLHCFREANEVADLLAKHSSLSRSSHLWEDSTP 181

Query: 152 DWIVSLVDSDCS 164
           D+I   + +D S
Sbjct: 182 DFISHAIINDLS 193

[Close]

BLAST of Spg023287 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 44.3 bits (103), Expect = 1.1e-04
Identity = 30/100 (30.00%), Postives = 49/100 (49.00%), Query Frame = 0


Query: 58  ELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVSKKSAPWGELEAELETIWSDVASFSDC 117
           EL+A+   +         +II ESD    ++L++     W  L+  LE I   +  F + 
Sbjct: 191 ELEALRWAVLTMSRFNYKRIIFESDAQALVNLLNSDDF-WPTLQPALEDIQQLLHHFEEV 250

Query: 118 SFIHTPKKKNLVADKIAKRARMLNSSDIWVFSI-SDWIVS 157
            F  TP+  N VAD+IA+ +   ++ D  +FSI   W+ S
Sbjct: 251 KFEFTPRGGNKVADRIARESISFSNYDPKLFSIVPQWLRS 289

[Close]

BLAST of Spg023287 vs. TAIR 10
Match: AT1G52990.1 (thioredoxin family protein )

HSP 1 Score: 42.4 bits (98), Expect = 4.4e-04
Identity = 29/89 (32.58%), Postives = 45/89 (50.56%), Query Frame = 0


Query: 58  ELKAIGEGIKLAKLLGCNKIIIESDCTQAIDLVSKKSAPWGELEAELETIWSDVASFSDC 117
           E  A+   I+     G  K+I E D +    L++ KS     L+  L+TI S + SF+  
Sbjct: 94  ECSALIWAIQATSAFGYTKVIFEGDNSNVNRLINTKSDN-PRLKHYLDTIKSWIPSFTST 153

Query: 118 SFIHTPKKKNLVADKIAKRARMLNSSDIW 147
            FI T +++N  AD + K+A  + SS  W
Sbjct: 154 EFIFTHREQNQCADTLVKKA--IKSSTQW 179

The following BLAST results are available for this feature:

BLAST of Spg023287 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038902513.1	5.0e-10	33.33	uncharacterized protein LOC120089172 [Benincasa hispida]	[more]
XP_023915006.1	8.5e-10	29.61	uncharacterized protein LOC112026546 [Quercus suber]	[more]
VAH20608.1	2.5e-09	33.33	unnamed protein product [Triticum turgidum subsp. durum]	[more]
EMS54402.1	5.5e-09	36.36	Ketohexokinase [Triticum urartu]	[more]
VAH65567.1	1.2e-08	34.85	unnamed protein product [Triticum turgidum subsp. durum]	[more]

BLAST of Spg023287 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Spg023287 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A446K5I3	1.2e-09	33.33	RNase H domain-containing protein OS=Triticum turgidum subsp. durum OX=4567 GN=T...	[more]
M8AHN8	1.6e-09	33.55	Zinc finger CCCH domain-containing protein 7 OS=Aegilops tauschii OX=37682 GN=F7...	[more]
M7Z366	2.7e-09	36.36	Ketohexokinase OS=Triticum urartu OX=4572 GN=TRIUR3_11649 PE=3 SV=1	[more]
A0A446NU09	6.0e-09	34.85	RNase H domain-containing protein OS=Triticum turgidum subsp. durum OX=4567 GN=T...	[more]
A0A446W4K1	1.9e-07	28.79	RNase H domain-containing protein OS=Triticum turgidum subsp. durum OX=4567 GN=T...	[more]

BLAST of Spg023287 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G34320.1	1.1e-04	30.00	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G52990.1	4.4e-04	32.58	thioredoxin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 27..137 e-value: 7.9E-17 score: 61.2
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 31..138 e-value: 1.7E-10 score: 43.1
None	No IPR available	PANTHER	PTHR33033:SF67	SUBFAMILY NOT NAMED	coord: 31..163
None	No IPR available	PANTHER	PTHR33033	POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATED	coord: 31..163
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 32..135 e-value: 1.23099E-16 score: 70.0356
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 55..140

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg023287.1	Spg023287.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0044237	cellular metabolic process
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity
molecular_function	GO:0016740	transferase activity