Homology
BLAST of Spg023281 vs. NCBI nr
Match:
XP_023514261.1 (tubulin-folding cofactor D isoform X1 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 2285.4 bits (5921), Expect = 0.0e+00
Identity = 1178/1365 (86.30%), Postives = 1227/1365 (89.89%), Query Frame = 0
Query: 1 MAATTETAPKKEEVDGLITYDDDDEHESKERVIQKYFLQEWKLVKSILDDIVSNGCVSDI 60
MAATTETAPK EE DG++TYDDDDEHESKERVIQKYFLQEWKLVKSILDDI SNG VSD+
Sbjct: 1 MAATTETAPKNEEFDGMVTYDDDDEHESKERVIQKYFLQEWKLVKSILDDIASNGRVSDL 60
Query: 61 SAVHKIRSIMQASHSEVVRISSRDAYWAPFMDKYQEQGQLVEPYLDIIVSPLMSLVCSKA 120
S+VHKIRSI MDKYQEQGQLVEPYL+IIVSPLMSLVCSKA
Sbjct: 61 SSVHKIRSI---------------------MDKYQEQGQLVEPYLEIIVSPLMSLVCSKA 120
Query: 121 AELGVASDEVLEVIKPICIIIYTLVTVCGYKAVIRFFPHQVSDLELAVALLERCQNTNSV 180
EL VAS+EVLE IKPICIIIYTLVTVCGYKAVIRFFPHQVSDLELAV+LLERCQNTNSV
Sbjct: 121 TELDVASNEVLEAIKPICIIIYTLVTVCGYKAVIRFFPHQVSDLELAVSLLERCQNTNSV 180
Query: 181 TSLRQESTGEMEAQCVILLWLSILVLVPFDISTVDSSLSNASSLAKFEPSPLVLRIVSFC 240
T+LRQESTGEMEAQCVILLWLSILVLVPFDISTVDSSLS+ +SLAKFEPSPLVLRI+SFC
Sbjct: 181 TTLRQESTGEMEAQCVILLWLSILVLVPFDISTVDSSLSSTNSLAKFEPSPLVLRIISFC 240
Query: 241 KDYLSSAGPMRTMAGLLLARLLTRPDMPKAFISFTEWTHEALSATTNDVMDHFRLLGAAG 300
KDYL+SAGPMRTMAGLLLARLLTRPDMPKAFISF EWTHEALSATTND+MDHFRLLGAAG
Sbjct: 241 KDYLASAGPMRTMAGLLLARLLTRPDMPKAFISFIEWTHEALSATTNDIMDHFRLLGAAG 300
Query: 301 ALASIFKTGDRKLLLDVVPQVWNDTSMLIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHRA 360
ALASIFKTGDRKLLLDVVPQVWNDTS LIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHRA
Sbjct: 301 ALASIFKTGDRKLLLDVVPQVWNDTSTLIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHRA 360
Query: 361 ASWHYVLLSSSTVDRAKNLVLGVNCVLISKCASLVGCESRTSSLGDNISATVSVGTHKCS 420
ASWHYV SRTSSLGDNISA VSVGTHKC+
Sbjct: 361 ASWHYV--------------------------------SRTSSLGDNISAPVSVGTHKCT 420
Query: 421 PINAVELADVCGSSSSLEDEDMEVPEIIEEIIEMLLTGLKDTDTVVRWSAAKGLGRVTSR 480
PINAVEL+DVC SS+SLEDEDMEVPEIIEEIIE LLTGLKDTDTVVRWSAAKGLGRVTSR
Sbjct: 421 PINAVELSDVCQSSTSLEDEDMEVPEIIEEIIETLLTGLKDTDTVVRWSAAKGLGRVTSR 480
Query: 481 LTSALSEEVLSSVLELFSPGEGDGSWHGGCLALAELARRGLLLPYSLPQVVPIVVKEVEF 540
LTSALSEEVLSS+LELFSPGEGDGSWHGGCLALAELARRGLLLP+SLPQVVPIVVK
Sbjct: 481 LTSALSEEVLSSILELFSPGEGDGSWHGGCLALAELARRGLLLPHSLPQVVPIVVK---- 540
Query: 541 VIEVEFDNYSKIVDVFKWVFEEEVVMVKTVVEDVDMEVALHYDIRRGPHSVGSHVRDAAA 600
ALHYDIRRGPHSVGSHVRDAAA
Sbjct: 541 --------------------------------------ALHYDIRRGPHSVGSHVRDAAA 600
Query: 601 YVCWAFGRAYHHTDMREILKQLAPHLLTVACYDREVNCRRAAAAAFQENVGRQGNYPHGI 660
YVCWAFGRAYHHTDMREILKQLAPHLLTVACYDREVNCRRAAAAAFQENVGRQGNYPHGI
Sbjct: 601 YVCWAFGRAYHHTDMREILKQLAPHLLTVACYDREVNCRRAAAAAFQENVGRQGNYPHGI 660
Query: 661 DIVNTADYFSLASRVTSYMKVAVCIAQYEGYLLPFIDELLCNKICHWDKGLRELAADALS 720
DIVNTADYFSLASRVTSY+KVAVCI+QYEG+LLPFIDELLCNKICHWDKGLRELAADALS
Sbjct: 661 DIVNTADYFSLASRVTSYLKVAVCISQYEGFLLPFIDELLCNKICHWDKGLRELAADALS 720
Query: 721 ALVKYDPEYFASYAVEKLIPCTLSSDLCMRHGATLAVGEVVLSLHQCGHILSSDIQKRVA 780
ALVKYDPEYFASYAVEKLIPCTLSSDLCMRHGATLAVGEVVLSLHQCGHILSSDIQKRVA
Sbjct: 721 ALVKYDPEYFASYAVEKLIPCTLSSDLCMRHGATLAVGEVVLSLHQCGHILSSDIQKRVA 780
Query: 781 GIVPAIEKARLYRGKGGEIMRAAVSRFIECISLSHLPLPEKTKRTLLDALNENLRHPNSQ 840
GIVPAIEKARLYRGKGGEIMRAAVSRFIECISLSHLPL EKTKR LLDALNENLRHPNSQ
Sbjct: 781 GIVPAIEKARLYRGKGGEIMRAAVSRFIECISLSHLPLLEKTKRMLLDALNENLRHPNSQ 840
Query: 841 IQNAAVKSLKPFVPAYLATADAGKSGNVATKYVEQLSDPNVAVRRGSALALSVLPYEFLA 900
IQNAAVKSLKPFV AYLATADAGKSGN+ TKY+EQLSDPNVA+RRGSALALSVLPYEFLA
Sbjct: 841 IQNAAVKSLKPFVLAYLATADAGKSGNITTKYLEQLSDPNVAIRRGSALALSVLPYEFLA 900
Query: 901 NRWKDVLLKLCCSCAIEDNPDDRDAEARVNAVRGLVSVCETLVQGRECSNEDDVPLLYLI 960
NRWKDVLLKLCCSCAIEDNPDDRDAEARVNAVRGLVSVCETLVQGRE SN D++PLLYLI
Sbjct: 901 NRWKDVLLKLCCSCAIEDNPDDRDAEARVNAVRGLVSVCETLVQGREFSNVDELPLLYLI 960
Query: 961 KDEVMASLFKALDDYSVDNRGDVGSWVREAAMNGLEKCTYILCARDSNGFPKKLNGVGSE 1020
KDEVMASLFKALDDYSVDNRGDVGSWVREAAM GLEKCTYILCA DSNGF KKLN VGSE
Sbjct: 961 KDEVMASLFKALDDYSVDNRGDVGSWVREAAMIGLEKCTYILCAIDSNGFTKKLNSVGSE 1020
Query: 1021 PESHHCEMAEKDQIDSFFDSTMATSLVGGICKQAVEKLDKLREAAAMVLQRILYNKIIHV 1080
ES HCEMA+KDQ +SFFDS+MATSLVGGICKQAVEKLDKLREAAAMVLQRILYNK+I V
Sbjct: 1021 QESLHCEMADKDQTNSFFDSSMATSLVGGICKQAVEKLDKLREAAAMVLQRILYNKVILV 1080
Query: 1081 PHIPFQEILEEIVPDDPDMKWGVPAITYPRFVRLLQFGCYSKTVMSGLVISIGGMQDSLS 1140
PHIPF++ LEEI+PDDP+MKW VPA++YP FVRLLQFGCYSKTVMSGLVISIGGMQDSLS
Sbjct: 1081 PHIPFRDTLEEIIPDDPNMKWAVPAVSYPHFVRLLQFGCYSKTVMSGLVISIGGMQDSLS 1140
Query: 1141 KASMSALMEYLEGDAIGDQDKSSRKGILFTDLLWILQRYKKCDRVIIPTFKTIEILFSKR 1200
KAS+SALMEYLEGDAIGD DK SRKG+LFTDLLWILQRYKKCDRVIIPTFKTIEILFSKR
Sbjct: 1141 KASISALMEYLEGDAIGDLDKRSRKGMLFTDLLWILQRYKKCDRVIIPTFKTIEILFSKR 1200
Query: 1201 ILLNVEVHISSFCNGILDSMEIELKGSKDFSKLYAGIAILGYIASLTEPVNSRAFFHLLT 1260
ILLN+EVH+SSFCNGILDS+EIE+KGSKDFSKLYAGI+ILGYI SL+EPVNSRAF HLLT
Sbjct: 1201 ILLNMEVHLSSFCNGILDSLEIEMKGSKDFSKLYAGISILGYITSLSEPVNSRAFVHLLT 1260
Query: 1261 FLSHRYPKIRKVSAEQVYLVLLQNGDFVPENKIEQALEIVSNTCWEGDLENAKLQRRELS 1320
FLSHRYPKIRK SAEQVYLVLLQNGDFVPE+KIEQALEIVSNTCWEGDLENAKLQR+ELS
Sbjct: 1261 FLSHRYPKIRKASAEQVYLVLLQNGDFVPEDKIEQALEIVSNTCWEGDLENAKLQRQELS 1266
Query: 1321 DIAGIETDLRPKTNTVPPPEKEVKNRFSAADENASYSSLVESTGF 1366
DIAGIE +L P V PEKEVKNRFSA+DENA+YSSLVESTGF
Sbjct: 1321 DIAGIEINLHP----VRAPEKEVKNRFSASDENATYSSLVESTGF 1266
BLAST of Spg023281 vs. NCBI nr
Match:
XP_023000016.1 (tubulin-folding cofactor D isoform X1 [Cucurbita maxima])
HSP 1 Score: 2275.4 bits (5895), Expect = 0.0e+00
Identity = 1174/1365 (86.01%), Postives = 1224/1365 (89.67%), Query Frame = 0
Query: 1 MAATTETAPKKEEVDGLITYDDDDEHESKERVIQKYFLQEWKLVKSILDDIVSNGCVSDI 60
MAATTETAPK EE DG++TYDDDDEHESKERVIQKYFLQEWKLVKSILDDI SNG VSDI
Sbjct: 1 MAATTETAPKNEEFDGMVTYDDDDEHESKERVIQKYFLQEWKLVKSILDDIASNGRVSDI 60
Query: 61 SAVHKIRSIMQASHSEVVRISSRDAYWAPFMDKYQEQGQLVEPYLDIIVSPLMSLVCSKA 120
S+VHKIRSI MDKYQEQGQLVEPYL+IIVSPLMSLVCSKA
Sbjct: 61 SSVHKIRSI---------------------MDKYQEQGQLVEPYLEIIVSPLMSLVCSKA 120
Query: 121 AELGVASDEVLEVIKPICIIIYTLVTVCGYKAVIRFFPHQVSDLELAVALLERCQNTNSV 180
EL VAS++VLE IKPICIIIYTLVTVCGYKAVIRFFPHQVSDLELAV+LLERCQNTNSV
Sbjct: 121 TELDVASNKVLEAIKPICIIIYTLVTVCGYKAVIRFFPHQVSDLELAVSLLERCQNTNSV 180
Query: 181 TSLRQESTGEMEAQCVILLWLSILVLVPFDISTVDSSLSNASSLAKFEPSPLVLRIVSFC 240
T+LRQESTGEMEAQCVILLWLSILVLVPFDISTVDSSLS+ +SLAKFEPSPLVLRI+SFC
Sbjct: 181 TTLRQESTGEMEAQCVILLWLSILVLVPFDISTVDSSLSSTNSLAKFEPSPLVLRIISFC 240
Query: 241 KDYLSSAGPMRTMAGLLLARLLTRPDMPKAFISFTEWTHEALSATTNDVMDHFRLLGAAG 300
KDYLSSAGPMRTMAGLLLARLLTRPDMPKAFISF EWTHEALSATTND+MDHFRLLGAAG
Sbjct: 241 KDYLSSAGPMRTMAGLLLARLLTRPDMPKAFISFIEWTHEALSATTNDIMDHFRLLGAAG 300
Query: 301 ALASIFKTGDRKLLLDVVPQVWNDTSMLIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHRA 360
ALASIFKTGDRKLLLDVVPQVWNDTS LIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHRA
Sbjct: 301 ALASIFKTGDRKLLLDVVPQVWNDTSTLIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHRA 360
Query: 361 ASWHYVLLSSSTVDRAKNLVLGVNCVLISKCASLVGCESRTSSLGDNISATVSVGTHKCS 420
ASWHYV SRTSSLGDNISA VSVGTH C+
Sbjct: 361 ASWHYV--------------------------------SRTSSLGDNISAPVSVGTHTCT 420
Query: 421 PINAVELADVCGSSSSLEDEDMEVPEIIEEIIEMLLTGLKDTDTVVRWSAAKGLGRVTSR 480
PINAV+L+DVC SS+SLEDEDMEVPEIIEEIIE LLTGLKDTDTVVRWSAAKGLGRVTSR
Sbjct: 421 PINAVKLSDVCQSSTSLEDEDMEVPEIIEEIIETLLTGLKDTDTVVRWSAAKGLGRVTSR 480
Query: 481 LTSALSEEVLSSVLELFSPGEGDGSWHGGCLALAELARRGLLLPYSLPQVVPIVVKEVEF 540
LTSALSEEVLSS+LELFSPGEGDGSWHGGCLALAELARRGLLLP SLPQVVPIVVK
Sbjct: 481 LTSALSEEVLSSILELFSPGEGDGSWHGGCLALAELARRGLLLPLSLPQVVPIVVK---- 540
Query: 541 VIEVEFDNYSKIVDVFKWVFEEEVVMVKTVVEDVDMEVALHYDIRRGPHSVGSHVRDAAA 600
ALHYDIRRGPHSVGSHVRDAAA
Sbjct: 541 --------------------------------------ALHYDIRRGPHSVGSHVRDAAA 600
Query: 601 YVCWAFGRAYHHTDMREILKQLAPHLLTVACYDREVNCRRAAAAAFQENVGRQGNYPHGI 660
YVCWAFGRAYHHTDMR+ILKQLAPHLLTVACYDREVNCRRAAAAAFQENVGRQGNYPHGI
Sbjct: 601 YVCWAFGRAYHHTDMRDILKQLAPHLLTVACYDREVNCRRAAAAAFQENVGRQGNYPHGI 660
Query: 661 DIVNTADYFSLASRVTSYMKVAVCIAQYEGYLLPFIDELLCNKICHWDKGLRELAADALS 720
DIVNTADYFSLASRVTSY+KVAVCI+QYEG+LLPFIDELLCNKICHWDKGLRELAADALS
Sbjct: 661 DIVNTADYFSLASRVTSYLKVAVCISQYEGFLLPFIDELLCNKICHWDKGLRELAADALS 720
Query: 721 ALVKYDPEYFASYAVEKLIPCTLSSDLCMRHGATLAVGEVVLSLHQCGHILSSDIQKRVA 780
ALVKYDPEYFASYAVEKLIPCTLSSDLCMRHGATLAVGEVVLSLHQCGHILSSDIQKRVA
Sbjct: 721 ALVKYDPEYFASYAVEKLIPCTLSSDLCMRHGATLAVGEVVLSLHQCGHILSSDIQKRVA 780
Query: 781 GIVPAIEKARLYRGKGGEIMRAAVSRFIECISLSHLPLPEKTKRTLLDALNENLRHPNSQ 840
GIVPAIEKARLYRGKGGEIMRAAVSRFIECISLSHLPL EKTKR LLDALNENLRHPNSQ
Sbjct: 781 GIVPAIEKARLYRGKGGEIMRAAVSRFIECISLSHLPLLEKTKRMLLDALNENLRHPNSQ 840
Query: 841 IQNAAVKSLKPFVPAYLATADAGKSGNVATKYVEQLSDPNVAVRRGSALALSVLPYEFLA 900
IQNAAVKSLKPFV AYLATADAGKSGN+ TKY+EQLSDPNVAVRRGSALALSVLPYEFLA
Sbjct: 841 IQNAAVKSLKPFVLAYLATADAGKSGNITTKYLEQLSDPNVAVRRGSALALSVLPYEFLA 900
Query: 901 NRWKDVLLKLCCSCAIEDNPDDRDAEARVNAVRGLVSVCETLVQGRECSNEDDVPLLYLI 960
NRWKDVLLKLCCSCAIEDNPDDRDAEARVNAVRGLVSVCETLVQGRE SN D++PLLYLI
Sbjct: 901 NRWKDVLLKLCCSCAIEDNPDDRDAEARVNAVRGLVSVCETLVQGREFSNVDELPLLYLI 960
Query: 961 KDEVMASLFKALDDYSVDNRGDVGSWVREAAMNGLEKCTYILCARDSNGFPKKLNGVGSE 1020
KDEVMASLFKALDDYSVDNRGDVGSWVREAAM GLEKCTYILCARDSNGF KKLN VGSE
Sbjct: 961 KDEVMASLFKALDDYSVDNRGDVGSWVREAAMIGLEKCTYILCARDSNGFTKKLNSVGSE 1020
Query: 1021 PESHHCEMAEKDQIDSFFDSTMATSLVGGICKQAVEKLDKLREAAAMVLQRILYNKIIHV 1080
ES HCEM+EKDQ +SFFDS MATSLVGGICKQAVEKLDKLREAAAMVL RILY+K+I V
Sbjct: 1021 QESLHCEMSEKDQTNSFFDSAMATSLVGGICKQAVEKLDKLREAAAMVLLRILYSKVILV 1080
Query: 1081 PHIPFQEILEEIVPDDPDMKWGVPAITYPRFVRLLQFGCYSKTVMSGLVISIGGMQDSLS 1140
PHIPF++ LEEI+PDDP+MKW VPA++YP FVRLLQFGCYSKTVMSGLVISIGGMQDSLS
Sbjct: 1081 PHIPFRDTLEEIIPDDPNMKWAVPAVSYPHFVRLLQFGCYSKTVMSGLVISIGGMQDSLS 1140
Query: 1141 KASMSALMEYLEGDAIGDQDKSSRKGILFTDLLWILQRYKKCDRVIIPTFKTIEILFSKR 1200
KAS+SALMEYLEGDAI DQDK SRKG+LFTDL+WILQRYKKCDRVIIPTFKTIEILFSKR
Sbjct: 1141 KASISALMEYLEGDAIEDQDKRSRKGMLFTDLIWILQRYKKCDRVIIPTFKTIEILFSKR 1200
Query: 1201 ILLNVEVHISSFCNGILDSMEIELKGSKDFSKLYAGIAILGYIASLTEPVNSRAFFHLLT 1260
ILLN+EVH+SSFCNGILDS+EIE+KGSKDFSKLYAGI+ILGYI SL+EPVNSRAF HLL+
Sbjct: 1201 ILLNMEVHLSSFCNGILDSLEIEMKGSKDFSKLYAGISILGYITSLSEPVNSRAFVHLLS 1260
Query: 1261 FLSHRYPKIRKVSAEQVYLVLLQNGDFVPENKIEQALEIVSNTCWEGDLENAKLQRRELS 1320
FLSHRYPKIRK SAEQVYLVLLQNGDFVPE+KIEQALEIVSNTCWEGDLENAKLQR+ELS
Sbjct: 1261 FLSHRYPKIRKASAEQVYLVLLQNGDFVPEDKIEQALEIVSNTCWEGDLENAKLQRQELS 1266
Query: 1321 DIAGIETDLRPKTNTVPPPEKEVKNRFSAADENASYSSLVESTGF 1366
DIAGIE +L P V PEKEVKNRFSA+DENA+YSSLVESTGF
Sbjct: 1321 DIAGIEINLHP----VRAPEKEVKNRFSASDENATYSSLVESTGF 1266
BLAST of Spg023281 vs. NCBI nr
Match:
XP_038906763.1 (tubulin-folding cofactor D [Benincasa hispida])
HSP 1 Score: 2275.0 bits (5894), Expect = 0.0e+00
Identity = 1172/1366 (85.80%), Postives = 1217/1366 (89.09%), Query Frame = 0
Query: 1 MAATTETA-PKKEEVDGLITYDDDDEHESKERVIQKYFLQEWKLVKSILDDIVSNGCVSD 60
MAATTE A PKKEE+DG+ TYDDDDEHESKERVIQKYFLQEWKLVKSILDDIVSNGCV D
Sbjct: 1 MAATTEAASPKKEELDGMTTYDDDDEHESKERVIQKYFLQEWKLVKSILDDIVSNGCVYD 60
Query: 61 ISAVHKIRSIMQASHSEVVRISSRDAYWAPFMDKYQEQGQLVEPYLDIIVSPLMSLVCSK 120
IS+VHKIRSI MDKYQEQGQLVEPYL+IIVSPLMSLVCSK
Sbjct: 61 ISSVHKIRSI---------------------MDKYQEQGQLVEPYLEIIVSPLMSLVCSK 120
Query: 121 AAELGVASDEVLEVIKPICIIIYTLVTVCGYKAVIRFFPHQVSDLELAVALLERCQNTNS 180
A LGV SDE+LEVIKPICIIIYTLVTVCGYKAVIRFFPHQVSDLELAV+LLERCQNTNS
Sbjct: 121 ATALGVDSDELLEVIKPICIIIYTLVTVCGYKAVIRFFPHQVSDLELAVSLLERCQNTNS 180
Query: 181 VTSLRQESTGEMEAQCVILLWLSILVLVPFDISTVDSSLSNASSLAKFEPSPLVLRIVSF 240
VTS RQESTGEMEAQCVILLWLSILVLVPFDISTVDSSLSN + LAKFEP+PLVLRI+SF
Sbjct: 181 VTSSRQESTGEMEAQCVILLWLSILVLVPFDISTVDSSLSNTNDLAKFEPAPLVLRIISF 240
Query: 241 CKDYLSSAGPMRTMAGLLLARLLTRPDMPKAFISFTEWTHEALSATTNDVMDHFRLLGAA 300
CKDYLSSAGPMRTMAGLLLARLLTRPDMPKAFISFTEWTHEALSATTND+M+HFRLLGA
Sbjct: 241 CKDYLSSAGPMRTMAGLLLARLLTRPDMPKAFISFTEWTHEALSATTNDIMNHFRLLGAT 300
Query: 301 GALASIFKTGDRKLLLDVVPQVWNDTSMLIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHR 360
GALASIFKTGDRKLLLDVVPQVWNDTSMLIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHR
Sbjct: 301 GALASIFKTGDRKLLLDVVPQVWNDTSMLIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHR 360
Query: 361 AASWHYVLLSSSTVDRAKNLVLGVNCVLISKCASLVGCESRTSSLGDNISATVSVGTHKC 420
AASWHYV SRTSSLGDNISAT+SVGT KC
Sbjct: 361 AASWHYV--------------------------------SRTSSLGDNISATMSVGTQKC 420
Query: 421 SPINAVELADVCGSSSSLEDEDMEVPEIIEEIIEMLLTGLKDTDTVVRWSAAKGLGRVTS 480
SPINA EL+ VC SS SLEDEDMEVPEIIEEIIEMLLTGLKDTDTVVRWSAAKGLGRVTS
Sbjct: 421 SPINAAELSYVCQSSISLEDEDMEVPEIIEEIIEMLLTGLKDTDTVVRWSAAKGLGRVTS 480
Query: 481 RLTSALSEEVLSSVLELFSPGEGDGSWHGGCLALAELARRGLLLPYSLPQVVPIVVKEVE 540
RLTS LSEEVLSS+LELFSPGEGDGSWHGGCLA+AELARRGLLLP SLPQVVPIVVK
Sbjct: 481 RLTSTLSEEVLSSILELFSPGEGDGSWHGGCLAVAELARRGLLLPCSLPQVVPIVVK--- 540
Query: 541 FVIEVEFDNYSKIVDVFKWVFEEEVVMVKTVVEDVDMEVALHYDIRRGPHSVGSHVRDAA 600
ALHYDIRRGPHSVGSHVRDAA
Sbjct: 541 ---------------------------------------ALHYDIRRGPHSVGSHVRDAA 600
Query: 601 AYVCWAFGRAYHHTDMREILKQLAPHLLTVACYDREVNCRRAAAAAFQENVGRQGNYPHG 660
AYVCWAFGRAYHHTDM+EILKQLAPHLLTVACYDREVNCRRAAAAAFQENVGRQGNYPHG
Sbjct: 601 AYVCWAFGRAYHHTDMKEILKQLAPHLLTVACYDREVNCRRAAAAAFQENVGRQGNYPHG 660
Query: 661 IDIVNTADYFSLASRVTSYMKVAVCIAQYEGYLLPFIDELLCNKICHWDKGLRELAADAL 720
IDIVNTADYFSLASRVTSY+KVAVCIAQYEGYLLPFIDELLCNKICHWDKGLRELAADAL
Sbjct: 661 IDIVNTADYFSLASRVTSYVKVAVCIAQYEGYLLPFIDELLCNKICHWDKGLRELAADAL 720
Query: 721 SALVKYDPEYFASYAVEKLIPCTLSSDLCMRHGATLAVGEVVLSLHQCGHILSSDIQKRV 780
SALVKYDPEYF SY VEKLIPCTLSSDLCMRHGATLAVGEVVLSLHQC HILSSDIQKRV
Sbjct: 721 SALVKYDPEYFESYVVEKLIPCTLSSDLCMRHGATLAVGEVVLSLHQCRHILSSDIQKRV 780
Query: 781 AGIVPAIEKARLYRGKGGEIMRAAVSRFIECISLSHLPLPEKTKRTLLDALNENLRHPNS 840
AGIVPAIEKARLYRGKGGEIMRAAVSRFIECISLS+LPL EKTKR LLDALNENLRHPNS
Sbjct: 781 AGIVPAIEKARLYRGKGGEIMRAAVSRFIECISLSNLPLLEKTKRMLLDALNENLRHPNS 840
Query: 841 QIQNAAVKSLKPFVPAYLATADAGKSGNVATKYVEQLSDPNVAVRRGSALALSVLPYEFL 900
IQNAAVK+LKPFV AYL AD GKSGN+ TKY+EQL+DPNVAVRRGSALALSVLPYE L
Sbjct: 841 HIQNAAVKALKPFVSAYLVAADTGKSGNITTKYLEQLNDPNVAVRRGSALALSVLPYELL 900
Query: 901 ANRWKDVLLKLCCSCAIEDNPDDRDAEARVNAVRGLVSVCETLVQGRECSNEDDVPLLYL 960
ANRWKDVLLKL C+CAIEDNPDDRDAEARVNAVRGLVSVCETLVQG+ECSNED +PLL+L
Sbjct: 901 ANRWKDVLLKLSCACAIEDNPDDRDAEARVNAVRGLVSVCETLVQGKECSNEDGIPLLFL 960
Query: 961 IKDEVMASLFKALDDYSVDNRGDVGSWVREAAMNGLEKCTYILCARDSNGFPKKLNGVGS 1020
IKDEVM SLFKALDDYSVDNRGDVGSWVREAAMNGLEKCTYILCAR NGF K LNGVG
Sbjct: 961 IKDEVMTSLFKALDDYSVDNRGDVGSWVREAAMNGLEKCTYILCARGPNGFSKTLNGVGF 1020
Query: 1021 EPESHHCEMAEKDQIDSFFDSTMATSLVGGICKQAVEKLDKLREAAAMVLQRILYNKIIH 1080
EPES HCE A KDQ SFFDSTMATSLVGG+CKQAVEKLDKLRE AA VLQRILYNKIIH
Sbjct: 1021 EPESLHCEKAAKDQTTSFFDSTMATSLVGGLCKQAVEKLDKLREVAATVLQRILYNKIIH 1080
Query: 1081 VPHIPFQEILEEIVPDDPDMKWGVPAITYPRFVRLLQFGCYSKTVMSGLVISIGGMQDSL 1140
VPHIPF+EILE+IVPDDP MKWGVPA++YPRFVRLLQF CYSKTVMSGLVISIGGMQDSL
Sbjct: 1081 VPHIPFREILEKIVPDDPHMKWGVPAVSYPRFVRLLQFDCYSKTVMSGLVISIGGMQDSL 1140
Query: 1141 SKASMSALMEYLEGDAIGDQDKSSRKGILFTDLLWILQRYKKCDRVIIPTFKTIEILFSK 1200
SKASMS+LMEYLEGDAIGDQD+SSRKG+LFTDLLWILQRYK+CDRVI+PTFKTIEILFSK
Sbjct: 1141 SKASMSSLMEYLEGDAIGDQDESSRKGMLFTDLLWILQRYKRCDRVIVPTFKTIEILFSK 1200
Query: 1201 RILLNVEVHISSFCNGILDSMEIELKGSKDFSKLYAGIAILGYIASLTEPVNSRAFFHLL 1260
RILLN+EVHISSFCNGIL S+EIELKGSKDFSKLYAGIAILGYIASL+EPVNSRAF +LL
Sbjct: 1201 RILLNMEVHISSFCNGILVSLEIELKGSKDFSKLYAGIAILGYIASLSEPVNSRAFSYLL 1260
Query: 1261 TFLSHRYPKIRKVSAEQVYLVLLQNGDFVPENKIEQALEIVSNTCWEGDLENAKLQRREL 1320
TFLSHRYPKIRK SAEQVYLVLLQNGDFVPENKIE+ALEIVSNTCWEGDLENAKLQRREL
Sbjct: 1261 TFLSHRYPKIRKASAEQVYLVLLQNGDFVPENKIEEALEIVSNTCWEGDLENAKLQRREL 1271
Query: 1321 SDIAGIETDLRPKTNTVPPPEKEVKNRFSAADENASYSSLVESTGF 1366
S IAG ETD++PKTNT+PPPEK+VKNRFS ADENASY SLVESTGF
Sbjct: 1321 SYIAGTETDIQPKTNTMPPPEKQVKNRFSGADENASYLSLVESTGF 1271
BLAST of Spg023281 vs. NCBI nr
Match:
XP_022964337.1 (tubulin-folding cofactor D isoform X1 [Cucurbita moschata])
HSP 1 Score: 2275.0 bits (5894), Expect = 0.0e+00
Identity = 1174/1365 (86.01%), Postives = 1222/1365 (89.52%), Query Frame = 0
Query: 1 MAATTETAPKKEEVDGLITYDDDDEHESKERVIQKYFLQEWKLVKSILDDIVSNGCVSDI 60
MAA TETAPK EE DG++TYDDDDEHESKERVIQKYFLQEWKLVKSILDDI SNG VSDI
Sbjct: 1 MAAKTETAPKNEEFDGMVTYDDDDEHESKERVIQKYFLQEWKLVKSILDDIASNGRVSDI 60
Query: 61 SAVHKIRSIMQASHSEVVRISSRDAYWAPFMDKYQEQGQLVEPYLDIIVSPLMSLVCSKA 120
S+VHKIRSI MDKYQEQGQLVEPYL+IIVSPLMSLVCSKA
Sbjct: 61 SSVHKIRSI---------------------MDKYQEQGQLVEPYLEIIVSPLMSLVCSKA 120
Query: 121 AELGVASDEVLEVIKPICIIIYTLVTVCGYKAVIRFFPHQVSDLELAVALLERCQNTNSV 180
EL VAS+EVLE IKPICIIIYTLVTVCGYKAVIRFFPHQVSDLELAV+LLERCQNTNSV
Sbjct: 121 IELDVASNEVLEAIKPICIIIYTLVTVCGYKAVIRFFPHQVSDLELAVSLLERCQNTNSV 180
Query: 181 TSLRQESTGEMEAQCVILLWLSILVLVPFDISTVDSSLSNASSLAKFEPSPLVLRIVSFC 240
T+LRQESTGEMEAQCVILLWLSILVLVPFDISTVDSSLS+ +SLAKFEPSPLVLRI+SFC
Sbjct: 181 TTLRQESTGEMEAQCVILLWLSILVLVPFDISTVDSSLSSTNSLAKFEPSPLVLRIISFC 240
Query: 241 KDYLSSAGPMRTMAGLLLARLLTRPDMPKAFISFTEWTHEALSATTNDVMDHFRLLGAAG 300
KDYL+SAGPMRTMAGLLLARLLTRPDMPKAFISF EWTHEALSA TND+MDHFRLLGAAG
Sbjct: 241 KDYLASAGPMRTMAGLLLARLLTRPDMPKAFISFIEWTHEALSAATNDIMDHFRLLGAAG 300
Query: 301 ALASIFKTGDRKLLLDVVPQVWNDTSMLIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHRA 360
ALASIFKTGDRKLLLDVVPQVWNDTS LIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHRA
Sbjct: 301 ALASIFKTGDRKLLLDVVPQVWNDTSTLIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHRA 360
Query: 361 ASWHYVLLSSSTVDRAKNLVLGVNCVLISKCASLVGCESRTSSLGDNISATVSVGTHKCS 420
ASWHYV SRTSSLGDNISA VSVGTHKC+
Sbjct: 361 ASWHYV--------------------------------SRTSSLGDNISAPVSVGTHKCT 420
Query: 421 PINAVELADVCGSSSSLEDEDMEVPEIIEEIIEMLLTGLKDTDTVVRWSAAKGLGRVTSR 480
PINAVEL+DVC SS+SLEDEDMEVPEIIEEIIE LLTGLKDTDTVVRWSAAKGLGRVTSR
Sbjct: 421 PINAVELSDVCQSSTSLEDEDMEVPEIIEEIIETLLTGLKDTDTVVRWSAAKGLGRVTSR 480
Query: 481 LTSALSEEVLSSVLELFSPGEGDGSWHGGCLALAELARRGLLLPYSLPQVVPIVVKEVEF 540
LTSALSEEVLSS+LELFSPGEGDGSWHGGCLALAELARRGLLLP+SLPQVVPIVVK
Sbjct: 481 LTSALSEEVLSSILELFSPGEGDGSWHGGCLALAELARRGLLLPHSLPQVVPIVVK---- 540
Query: 541 VIEVEFDNYSKIVDVFKWVFEEEVVMVKTVVEDVDMEVALHYDIRRGPHSVGSHVRDAAA 600
ALHYDIRRGPHSVGSHVRDAAA
Sbjct: 541 --------------------------------------ALHYDIRRGPHSVGSHVRDAAA 600
Query: 601 YVCWAFGRAYHHTDMREILKQLAPHLLTVACYDREVNCRRAAAAAFQENVGRQGNYPHGI 660
YVCWAFGRAYHHTDMREILKQLAPHLLTVACYDREVNCRRAAAAAFQENVGRQGNYPHGI
Sbjct: 601 YVCWAFGRAYHHTDMREILKQLAPHLLTVACYDREVNCRRAAAAAFQENVGRQGNYPHGI 660
Query: 661 DIVNTADYFSLASRVTSYMKVAVCIAQYEGYLLPFIDELLCNKICHWDKGLRELAADALS 720
DIVNTADYFSLASRVTSY+KVAVCI+QYEG+LLPFIDELLCNKICHWDKGLRELAADALS
Sbjct: 661 DIVNTADYFSLASRVTSYLKVAVCISQYEGFLLPFIDELLCNKICHWDKGLRELAADALS 720
Query: 721 ALVKYDPEYFASYAVEKLIPCTLSSDLCMRHGATLAVGEVVLSLHQCGHILSSDIQKRVA 780
ALVKYDPEYFASYAVEKLIPCTLSSDLCMRHGATLAVGEVVLSLHQCGHILSSDIQKRVA
Sbjct: 721 ALVKYDPEYFASYAVEKLIPCTLSSDLCMRHGATLAVGEVVLSLHQCGHILSSDIQKRVA 780
Query: 781 GIVPAIEKARLYRGKGGEIMRAAVSRFIECISLSHLPLPEKTKRTLLDALNENLRHPNSQ 840
GIVPAIEKARLYRGKGGEIMRAAVSRFIECISLSHLPL EKTKR LLDALNENLRHPNSQ
Sbjct: 781 GIVPAIEKARLYRGKGGEIMRAAVSRFIECISLSHLPLLEKTKRMLLDALNENLRHPNSQ 840
Query: 841 IQNAAVKSLKPFVPAYLATADAGKSGNVATKYVEQLSDPNVAVRRGSALALSVLPYEFLA 900
IQNAAVKSLKPFV AYLATADAGKSGN+ TKY+EQLSDPNVA+RRGSALALSVLPYEFLA
Sbjct: 841 IQNAAVKSLKPFVLAYLATADAGKSGNITTKYLEQLSDPNVAIRRGSALALSVLPYEFLA 900
Query: 901 NRWKDVLLKLCCSCAIEDNPDDRDAEARVNAVRGLVSVCETLVQGRECSNEDDVPLLYLI 960
NRWKDVLLKLCCSCAIEDNPDDRDAEARVNAVRGLVSVCETLVQGRE SN D++PLLYLI
Sbjct: 901 NRWKDVLLKLCCSCAIEDNPDDRDAEARVNAVRGLVSVCETLVQGREFSNVDELPLLYLI 960
Query: 961 KDEVMASLFKALDDYSVDNRGDVGSWVREAAMNGLEKCTYILCARDSNGFPKKLNGVGSE 1020
KDEVMASLFKALDDYSVDNRGDVGSWVREAAM GLEKCTYILCA DSNGF KKLN VGSE
Sbjct: 961 KDEVMASLFKALDDYSVDNRGDVGSWVREAAMIGLEKCTYILCAIDSNGFTKKLNSVGSE 1020
Query: 1021 PESHHCEMAEKDQIDSFFDSTMATSLVGGICKQAVEKLDKLREAAAMVLQRILYNKIIHV 1080
ES HCEMA+KDQ +SFFDS+MATSLVGGICKQAVEKLDKLREAAAMVLQRILYNK+I V
Sbjct: 1021 QESLHCEMADKDQTNSFFDSSMATSLVGGICKQAVEKLDKLREAAAMVLQRILYNKVILV 1080
Query: 1081 PHIPFQEILEEIVPDDPDMKWGVPAITYPRFVRLLQFGCYSKTVMSGLVISIGGMQDSLS 1140
PHIPF++ LEEI+PDDP+MKW VPA++YP FVRLLQFGCYSKTVMSGLVISIGGMQDSLS
Sbjct: 1081 PHIPFRDTLEEIIPDDPNMKWAVPAVSYPHFVRLLQFGCYSKTVMSGLVISIGGMQDSLS 1140
Query: 1141 KASMSALMEYLEGDAIGDQDKSSRKGILFTDLLWILQRYKKCDRVIIPTFKTIEILFSKR 1200
KAS+SALMEYLEGDAIGDQDK SRKG+LFTDLLWILQRYKKCDRVIIPTFK IEILFSKR
Sbjct: 1141 KASISALMEYLEGDAIGDQDKRSRKGMLFTDLLWILQRYKKCDRVIIPTFKAIEILFSKR 1200
Query: 1201 ILLNVEVHISSFCNGILDSMEIELKGSKDFSKLYAGIAILGYIASLTEPVNSRAFFHLLT 1260
ILLN+EVH+SSFCNGILDS+EIE+KGSKDFSKLYAGI+ILGYI SL+EPVNSRAF HLL+
Sbjct: 1201 ILLNMEVHLSSFCNGILDSLEIEMKGSKDFSKLYAGISILGYITSLSEPVNSRAFVHLLS 1260
Query: 1261 FLSHRYPKIRKVSAEQVYLVLLQNGDFVPENKIEQALEIVSNTCWEGDLENAKLQRRELS 1320
LSHRYPKIRK SAEQVYLVLLQNGDFVPE+KIEQALEIVSNTCWEGDLENAKLQR+ELS
Sbjct: 1261 LLSHRYPKIRKASAEQVYLVLLQNGDFVPEDKIEQALEIVSNTCWEGDLENAKLQRQELS 1266
Query: 1321 DIAGIETDLRPKTNTVPPPEKEVKNRFSAADENASYSSLVESTGF 1366
DIAGIE +L P V PEKEVKNR SA DENA+YSSLVESTGF
Sbjct: 1321 DIAGIEINLHP----VRAPEKEVKNRVSALDENATYSSLVESTGF 1266
BLAST of Spg023281 vs. NCBI nr
Match:
XP_004145065.1 (tubulin-folding cofactor D [Cucumis sativus] >KGN46255.1 hypothetical protein Csa_004849 [Cucumis sativus])
HSP 1 Score: 2269.2 bits (5879), Expect = 0.0e+00
Identity = 1166/1366 (85.36%), Postives = 1220/1366 (89.31%), Query Frame = 0
Query: 1 MAATTETAPKKEEVDGLITYDDDDEHESKERVIQKYFLQEWKLVKSILDDIVSNGCVSDI 60
MAATTE APKKEEVDG +YDDDDE+ESKERVIQKYFLQEWKLVKSILDDIVSNG VSDI
Sbjct: 1 MAATTEAAPKKEEVDGTTSYDDDDEYESKERVIQKYFLQEWKLVKSILDDIVSNGRVSDI 60
Query: 61 SAVHKIRSIMQASHSEVVRISSRDAYWAPFMDKYQEQGQLVEPYLDIIVSPLMSLVCSKA 120
S+VHKIRSI MDKYQEQGQLVEPYL+IIVSPLMSLVCSKA
Sbjct: 61 SSVHKIRSI---------------------MDKYQEQGQLVEPYLEIIVSPLMSLVCSKA 120
Query: 121 AELGVASDEVLEVIKPICIIIYTLVTVCGYKAVIRFFPHQVSDLELAVALLERCQNTNSV 180
LGV SD++LEVIKPICIIIYTLVTVCGYKAVIRFFPHQVSDLELAV+LLERCQNTNSV
Sbjct: 121 TALGVESDKLLEVIKPICIIIYTLVTVCGYKAVIRFFPHQVSDLELAVSLLERCQNTNSV 180
Query: 181 TSLRQESTGEMEAQCVILLWLSILVLVPFDISTVDSSLSNASSLAKFEPSPLVLRIVSFC 240
TS RQESTGEMEAQCVILLWLSILVLVPFDIS+VDSSLSN + LA+FEP+PLVLRI+SFC
Sbjct: 181 TSSRQESTGEMEAQCVILLWLSILVLVPFDISSVDSSLSNTNDLAEFEPAPLVLRIISFC 240
Query: 241 KDYLSSAGPMRTMAGLLLARLLTRPDMPKAFISFTEWTHEALSATTNDVMDHFRLLGAAG 300
KDYLSSAGPMRTMAGLLLARLLTRPDMPKAFISFTEWTHEALS TTND+M+HFRLLGAAG
Sbjct: 241 KDYLSSAGPMRTMAGLLLARLLTRPDMPKAFISFTEWTHEALSTTTNDIMNHFRLLGAAG 300
Query: 301 ALASIFKTGDRKLLLDVVPQVWNDTSMLIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHRA 360
ALASIFKTGDRKLLLDVVPQVWNDTSMLIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHRA
Sbjct: 301 ALASIFKTGDRKLLLDVVPQVWNDTSMLIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHRA 360
Query: 361 ASWHYVLLSSSTVDRAKNLVLGVNCVLISKCASLVGCESRTSSLGDNISATVSVGTHKCS 420
ASWHYV SRTSSLGDNISAT+SVGTHKCS
Sbjct: 361 ASWHYV--------------------------------SRTSSLGDNISATMSVGTHKCS 420
Query: 421 PINAVELADVCGSSSSLEDEDMEVPEIIEEIIEMLLTGLKDTDTVVRWSAAKGLGRVTSR 480
PINAVEL++VC S+SLEDEDMEVPEIIEEIIEMLLTGLKDTDTVVRWSAAKGLGRVTSR
Sbjct: 421 PINAVELSNVCQGSTSLEDEDMEVPEIIEEIIEMLLTGLKDTDTVVRWSAAKGLGRVTSR 480
Query: 481 LTSALSEEVLSSVLELFSPGEGDGSWHGGCLALAELARRGLLLPYSLPQVVPIVVKEVEF 540
LTS LSEEVL S+LELFSPGEGDGSWHGGCLALAELARRGLLLP SLPQVVPIVVK
Sbjct: 481 LTSTLSEEVLLSILELFSPGEGDGSWHGGCLALAELARRGLLLPCSLPQVVPIVVK---- 540
Query: 541 VIEVEFDNYSKIVDVFKWVFEEEVVMVKTVVEDVDMEVALHYDIRRGPHSVGSHVRDAAA 600
ALHYDIRRGPHSVGSHVRDAAA
Sbjct: 541 --------------------------------------ALHYDIRRGPHSVGSHVRDAAA 600
Query: 601 YVCWAFGRAYHHTDMREILKQLAPHLLTVACYDREVNCRRAAAAAFQENVGRQGNYPHGI 660
YVCWAFGRAYHHTDMREILKQLAPHLLTVACYDREVNCRRAAAAAFQENVGRQGNYPHGI
Sbjct: 601 YVCWAFGRAYHHTDMREILKQLAPHLLTVACYDREVNCRRAAAAAFQENVGRQGNYPHGI 660
Query: 661 DIVNTADYFSLASRVTSYMKVAVCIAQYEGYLLPFIDELLCNKICHWDKGLRELAADALS 720
DIVN+ADYFSLASRVTSY+KVAVCI QYEGYLLPFIDELLCNKICHWDKGLRELAADALS
Sbjct: 661 DIVNSADYFSLASRVTSYLKVAVCIVQYEGYLLPFIDELLCNKICHWDKGLRELAADALS 720
Query: 721 ALVKYDPEYFASYAVEKLIPCTLSSDLCMRHGATLAVGEVVLSLHQCGHILSSDIQKRVA 780
ALVKYDPEYFASYAVEKLIPCTLSSDLCMRHGATLAVGEVVLSLHQCGHIL SDIQKRVA
Sbjct: 721 ALVKYDPEYFASYAVEKLIPCTLSSDLCMRHGATLAVGEVVLSLHQCGHILPSDIQKRVA 780
Query: 781 GIVPAIEKARLYRGKGGEIMRAAVSRFIECISLSHLPLPEKTKRTLLDALNENLRHPNSQ 840
GIVPAIEKARLYRGKGGEIMRAAVSRFIECISLSHLPL EKTKR LLDALNENLRHPNSQ
Sbjct: 781 GIVPAIEKARLYRGKGGEIMRAAVSRFIECISLSHLPLLEKTKRMLLDALNENLRHPNSQ 840
Query: 841 IQNAAVKSLKPFVPAYLATADAGKSGNVATKYVEQLSDPNVAVRRGSALALSVLPYEFLA 900
IQNAAVKSLKPFVPAYL AD GKSGN+ TKY+EQLSDPNVAVRRGSALALSVLPYE LA
Sbjct: 841 IQNAAVKSLKPFVPAYLVAADTGKSGNIITKYLEQLSDPNVAVRRGSALALSVLPYELLA 900
Query: 901 NRWKDVLLKLCCSCAIEDNPDDRDAEARVNAVRGLVSVCETLVQGRECSNEDDVPLLYLI 960
NRWKDV++KLCC+CAIE+NPDDRDAEARVNAVRGLVSVCETLVQGRECSNED +PLL L+
Sbjct: 901 NRWKDVIMKLCCACAIEENPDDRDAEARVNAVRGLVSVCETLVQGRECSNEDGIPLLCLM 960
Query: 961 KDEVMASLFKALDDYSVDNRGDVGSWVREAAMNGLEKCTYILCARDSNGFPKKLNGVGSE 1020
KDEVM SLFKALDDYSVDNRGDVGSWVREAAMNGLEKCTYILCAR S GF K +N GSE
Sbjct: 961 KDEVMTSLFKALDDYSVDNRGDVGSWVREAAMNGLEKCTYILCARGSCGFTKTVNEFGSE 1020
Query: 1021 PESHHCEMAEKDQ-IDSFFDSTMATSLVGGICKQAVEKLDKLREAAAMVLQRILYNKIIH 1080
PE+ HCE AEKDQ SFFDSTMATSLVGGICKQAVEKLDKLREAAA +LQRILYNKI+H
Sbjct: 1021 PETLHCEKAEKDQTTTSFFDSTMATSLVGGICKQAVEKLDKLREAAATILQRILYNKIVH 1080
Query: 1081 VPHIPFQEILEEIVPDDPDMKWGVPAITYPRFVRLLQFGCYSKTVMSGLVISIGGMQDSL 1140
VPHIPF+EILE+IVPD+ DMKWGVPA++YPRFVRLLQFGCYSKTVMSGLV+S+GGMQDSL
Sbjct: 1081 VPHIPFREILEKIVPDNQDMKWGVPAVSYPRFVRLLQFGCYSKTVMSGLVVSVGGMQDSL 1140
Query: 1141 SKASMSALMEYLEGDAIGDQDKSSRKGILFTDLLWILQRYKKCDRVIIPTFKTIEILFSK 1200
SKASMSALMEYLEGDAIGDQD+SSRKG+LFTD+LWILQRYK+CDRVI+PTFKTIEILFSK
Sbjct: 1141 SKASMSALMEYLEGDAIGDQDESSRKGMLFTDILWILQRYKRCDRVIVPTFKTIEILFSK 1200
Query: 1201 RILLNVEVHISSFCNGILDSMEIELKGSKDFSKLYAGIAILGYIASLTEPVNSRAFFHLL 1260
RI LN+EVHISSFCNGIL S+++ELKGSKDFSKLYAGIAILGYIASL EPVNSRAF +LL
Sbjct: 1201 RI-LNMEVHISSFCNGILGSLDVELKGSKDFSKLYAGIAILGYIASLPEPVNSRAFSYLL 1260
Query: 1261 TFLSHRYPKIRKVSAEQVYLVLLQNGDFVPENKIEQALEIVSNTCWEGDLENAKLQRREL 1320
TFLSHRYPKIRK SAEQVYLVLLQNG+FVPENKI++ALEIVSNTCWEGDLENAKLQRREL
Sbjct: 1261 TFLSHRYPKIRKASAEQVYLVLLQNGNFVPENKIDEALEIVSNTCWEGDLENAKLQRREL 1270
Query: 1321 SDIAGIETDLRPKTNTVPPPEKEVKNRFSAADENASYSSLVESTGF 1366
DIAGIETD+ KTN VPPPEKEVKNRFS ADENASYSSLVESTGF
Sbjct: 1321 YDIAGIETDIHSKTNMVPPPEKEVKNRFSGADENASYSSLVESTGF 1270
BLAST of Spg023281 vs. ExPASy Swiss-Prot
Match:
Q8L5R3 (Tubulin-folding cofactor D OS=Arabidopsis thaliana OX=3702 GN=TFCD PE=2 SV=1)
HSP 1 Score: 1604.0 bits (4152), Expect = 0.0e+00
Identity = 847/1364 (62.10%), Postives = 1023/1364 (75.00%), Query Frame = 0
Query: 6 ETAPKKEEVDGL--ITYDDDDEHESKERVIQKYFLQEWKLVKSILDDIVSNGCVSDISAV 65
E +P K E++ I ++DDEH+SKERV+Q+YFLQEWKLVKS+LDDIVSNG V D ++V
Sbjct: 7 EMSPTKIEIETAVKIEEEEDDEHDSKERVLQRYFLQEWKLVKSLLDDIVSNGRVVDPTSV 66
Query: 66 HKIRSIMQASHSEVVRISSRDAYWAPFMDKYQEQGQLVEPYLDIIVSPLMSLVCSKAAEL 125
HKIRSI MDKYQEQGQLVEPYL+ IVSPLM ++ SK +L
Sbjct: 67 HKIRSI---------------------MDKYQEQGQLVEPYLESIVSPLMFIIRSKTVDL 126
Query: 126 GVASDEVLEVIKPICIIIYTLVTVCGYKAVIRFFPHQVSDLELAVALLERCQNTNSVTSL 185
DE+LE+IKPI IIIY LVTVCGYKAVI+FFPHQVSDLELAV LLE+C +TNSV++L
Sbjct: 127 EAKPDEILEIIKPISIIIYALVTVCGYKAVIKFFPHQVSDLELAVLLLEKCHSTNSVSAL 186
Query: 186 RQESTGEMEAQCVILLWLSILVLVPFDISTVDSSLSNASSLAKFEPSPLVLRIVSFCKDY 245
RQESTGEMEA+CV LLWLSILVLVPFDIS+VD+S+++ + + +PLVL+I+ FCKDY
Sbjct: 187 RQESTGEMEAKCVTLLWLSILVLVPFDISSVDTSIADDKTFGVDDLAPLVLKILGFCKDY 246
Query: 246 LSSAGPMRTMAGLLLARLLTRPDMPKAFISFTEWTHEALSATTNDVMDHFRLLGAAGALA 305
L SAGPMR ++GLLL++LLTRPDM KAF SF EWT+E LS + V +HFRLLG AL+
Sbjct: 247 LCSAGPMRRISGLLLSKLLTRPDMGKAFSSFFEWTYEVLSCKEDSVTNHFRLLGVMEALS 306
Query: 306 SIFKTGDRKLLLDVVPQVWNDTSMLIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHRAASW 365
+IFKT RK+LLDV+P V ND ++L KSN A +S LLRKYL+KLTQRIGL CLPHR+ SW
Sbjct: 307 AIFKTASRKVLLDVLPIVLNDVTVLSKSNAAAKSSLLRKYLIKLTQRIGLVCLPHRSPSW 366
Query: 366 HYVLLSSSTVDRAKNLVLGVNCVLISKCASLVGCESRTSSLGDNISATVSVGTHKCSPIN 425
YV ++T+SL +N+S + S + +P +
Sbjct: 367 RYV--------------------------------AQTASLSENMSTSSS---QRLAPDH 426
Query: 426 AVELADVCGSSSSLEDEDMEVPEIIEEIIEMLLTGLKDTDTVVRWSAAKGLGRVTSRLTS 485
V S EDEDM+VPEI+EEIIEMLL+GL+DTDTVVRWSAAKG+GRVTSRLTS
Sbjct: 427 TVTAILQPESLDDQEDEDMDVPEILEEIIEMLLSGLRDTDTVVRWSAAKGIGRVTSRLTS 486
Query: 486 ALSEEVLSSVLELFSPGEGDGSWHGGCLALAELARRGLLLPYSLPQVVPIVVKEVEFVIE 545
LS+EVLSSVLELFSPGEGDGSWHGGCLALAELARRGLLLP S P VVP++VK
Sbjct: 487 VLSDEVLSSVLELFSPGEGDGSWHGGCLALAELARRGLLLPRSFPLVVPVIVK------- 546
Query: 546 VEFDNYSKIVDVFKWVFEEEVVMVKTVVEDVDMEVALHYDIRRGPHSVGSHVRDAAAYVC 605
ALHYD+RRGPHSVGSHVRDAAAYVC
Sbjct: 547 -----------------------------------ALHYDVRRGPHSVGSHVRDAAAYVC 606
Query: 606 WAFGRAYHHTDMREILKQLAPHLLTVACYDREVNCRRAAAAAFQENVGRQGNYPHGIDIV 665
WAFGRAY H DM+ +L QLAP LL V +DREVNCRRAAAAAFQENVGRQGNYPHGIDIV
Sbjct: 607 WAFGRAYSHKDMKNVLDQLAPDLLIVGSFDREVNCRRAAAAAFQENVGRQGNYPHGIDIV 666
Query: 666 NTADYFSLASRVTSYMKVAVCIAQYEGYLLPFIDELLCNKICHWDKGLRELAADALSALV 725
+ ADYFSL+SRV SY++VAV IAQYEGYL PF+DELL NKICHWDK LRELAA+AL+ LV
Sbjct: 667 SIADYFSLSSRVNSYLQVAVSIAQYEGYLYPFVDELLYNKICHWDKSLRELAAEALAPLV 726
Query: 726 KYDPEYFASYAVEKLIPCTLSSDLCMRHGATLAVGEVVLSLHQCGHILSSDIQKRVAGIV 785
KY+P++FA+Y +EKLIPCTLS+DLCMRHGATLA GEVVL+LHQCG++LS+D QKR+AGIV
Sbjct: 727 KYEPKHFANYVLEKLIPCTLSTDLCMRHGATLAAGEVVLALHQCGYVLSADSQKRMAGIV 786
Query: 786 PAIEKARLYRGKGGEIMRAAVSRFIECISLSHLPLPEKTKRTLLDALNENLRHPNSQIQN 845
P+IEKARLYRGKGGEIMR AVSRFIECISLSH+ L E+T+R LLD L ENLRHPNSQIQN
Sbjct: 787 PSIEKARLYRGKGGEIMRLAVSRFIECISLSHVTLAERTERILLDTLTENLRHPNSQIQN 846
Query: 846 AAVKSLKPFVPAYLATADAGKSGNVATKYVEQLSDPNVAVRRGSALALSVLPYEFLANRW 905
AAV ++K V +YL D KS ++ K+++ L+DPNVAVRRGSALAL VLPYE L +W
Sbjct: 847 AAVSAVKQLVQSYLVGNDK-KSVDLILKHLKHLTDPNVAVRRGSALALGVLPYELLTAKW 906
Query: 906 KDVLLKLCCSCAIEDNPDDRDAEARVNAVRGLVSVCETLVQGRECS-NEDDVPLLYLIKD 965
KD++LKLC +C IE NP+DRDAEARVNAV+GL SVCETL Q R DD+ L LIK
Sbjct: 907 KDIVLKLCSACKIEVNPEDRDAEARVNAVKGLTSVCETLTQKRASDPGNDDLSLFLLIKT 966
Query: 966 EVMASLFKALDDYSVDNRGDVGSWVREAAMNGLEKCTYILCARDSNGFPKKLNGVGSEPE 1025
EVM +L KALDDYSVDNRGDVGSWVREAA++GLEKCTYILC KK+
Sbjct: 967 EVMDTLLKALDDYSVDNRGDVGSWVREAAVHGLEKCTYILC--------KKM-------- 1026
Query: 1026 SHHCEMAEKDQIDSFFDSTMATSLVGGICKQAVEKLDKLREAAAMVLQRILYNKIIHVPH 1085
+ E D S FDS +AT L+GG+ KQ VEK+DKLRE AA VLQRILY+K + VPH
Sbjct: 1027 ETYSEGDYNDDTSSLFDSNLATRLIGGMLKQGVEKMDKLRETAAKVLQRILYHKSVSVPH 1086
Query: 1086 IPFQEILEEIVPDDPDMKWGVPAITYPRFVRLLQFGCYSKTVMSGLVISIGGMQDSLSKA 1145
+P++E LEEI+P+ +++W VPA ++PRFV+LL+ CYSK VMSGLVISIGG+QDSL KA
Sbjct: 1087 VPYREKLEEILPNKANLQWAVPAFSFPRFVQLLKLRCYSKEVMSGLVISIGGLQDSLRKA 1146
Query: 1146 SMSALMEYL-EGDAIGDQDKSSRKGILFTDLLWILQRYKKCDRVIIPTFKTIEILFSKRI 1205
S+ AL+EY+ EG+A +++ SR+ L D+LWILQ YKKCDRV++P +TIEILFS +I
Sbjct: 1147 SLVALLEYMREGEAKDPKEQQSRESALGDDILWILQEYKKCDRVMVPCLQTIEILFSSKI 1206
Query: 1206 LLNVEVHISSFCNGILDSMEIELKGSKDFSKLYAGIAILGYIASLTEPVNSRAFFHLLTF 1265
LN E + SF G++DS+ IEL+ SKDF+KL AG+AILGYIAS++ ++++AF LL+F
Sbjct: 1207 FLNQESYTFSFYAGVMDSLAIELRASKDFTKLKAGLAILGYIASVSHFISTKAFSQLLSF 1254
Query: 1266 LSHRYPKIRKVSAEQVYLVLLQNGDFVPENKIEQALEIVSNTCWEGDLENAKLQRRELSD 1325
L HRYP IRK +AEQVYL LLQNG V E K+E+ +EI+S +CWE D+E K QR EL +
Sbjct: 1267 LGHRYPMIRKAAAEQVYLALLQNGILVTEEKMEKVIEIISESCWEADMETTKTQRLELCE 1254
Query: 1326 IAGIETDLRPKTNTVPPPEKEVKNRFSAADENASYSSLVESTGF 1366
+AG++ ++ KT K++ SAADENASYSSLV+S+GF
Sbjct: 1327 LAGLDHEVVFKTRN-RLVIKDIAGNKSAADENASYSSLVDSSGF 1254
BLAST of Spg023281 vs. ExPASy Swiss-Prot
Match:
Q9BTW9 (Tubulin-specific chaperone D OS=Homo sapiens OX=9606 GN=TBCD PE=1 SV=2)
HSP 1 Score: 603.2 bits (1554), Expect = 7.3e-171
Identity = 428/1286 (33.28%), Postives = 641/1286 (49.84%), Query Frame = 0
Query: 91 MDKYQEQGQLVEPYLDIIVSPLMSLVCSKAAELGVASDEVLEVIKPICIIIYTLVTVCGY 150
MDKYQEQ L++P+L+ +++ L+ +V + + ++ +Y + V GY
Sbjct: 64 MDKYQEQPHLLDPHLEWMMNLLLDIVQDQTSP--------ASLVHLAFKFLYIITKVRGY 123
Query: 151 KAVIRFFPHQVSDLELAVALLERCQNTNSVTSLRQESTGEMEAQCVILLWLSILVLVPFD 210
K +R FPH+V+D+E + L VT + E + ++LLWLS+ L+PFD
Sbjct: 124 KTFLRLFPHEVADVEPVLDL---------VTIQNPKDHEAWETRYMLLLWLSVTCLIPFD 183
Query: 211 ISTVDSSLSNASSLAKFEPSPLVLRIVSFCKDYLSSAGPMRTMAGLLLARLLTRPDMPKA 270
S +D +L A+ ++ RI+ + YL + R A +L++R +TRPD+ ++
Sbjct: 184 FSRLDGNLLTQPGQARMS---IMDRILQIAESYLIVSDKARDAAAVLVSRFITRPDVKQS 243
Query: 271 -FISFTEWTHEALSATTNDVMDH-FRLLGAAGALASIFKTGDRKLLLDVVPQVWN--DTS 330
F +W+ L+ ++ M + G ALA IFK G R+ L V D
Sbjct: 244 KMAEFLDWSLCNLARSSFQTMQGVITMDGTLQALAQIFKHGKREDCLPYAATVLRCLDGC 303
Query: 331 MLIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHRAASWHYVLLSSSTVDRAKNLVLGVNCV 390
L +SN LLRK +KL QR+GLT L + A+W Y
Sbjct: 304 RLPESN----QTLLRKLGVKLVQRLGLTFLKPKVAAWRYQR------------------- 363
Query: 391 LISKCASLVGCESRTSSLGDNISATVSVGTHKCSPINAVELADVCGSSSSLEDEDMEVPE 450
GC S ++L ++ G + P+ E +DED +VPE
Sbjct: 364 ---------GCRSLAANL-----QLLTQGQSEQKPLILTE----------DDDEDDDVPE 423
Query: 451 IIEEIIEMLLTGLKDTDTVVRWSAAKGLGRVTSRLTSALSEEVLSSVLELFSPGEGDGSW 510
+E +IE LL GLKD DTVVRWSAAKG+GR+ RL AL+++V+ SVL+ FS E D +W
Sbjct: 424 GVERVIEQLLVGLKDKDTVVRWSAAKGIGRMAGRLPRALADDVVGSVLDCFSFQETDKAW 483
Query: 511 HGGCLALAELARRGLLLPYSLPQVVPIVVKEVEFVIEVEFDNYSKIVDVFKWVFEEEVVM 570
HGGCLALAEL RRGLLLP L VV +++K
Sbjct: 484 HGGCLALAELGRRGLLLPSRLVDVVAVILK------------------------------ 543
Query: 571 VKTVVEDVDMEVALHYDIRRGPHSVGSHVRDAAAYVCWAFGRAYHHTDMREILKQLAPHL 630
AL YD +RG SVG++VRDAA YVCWAF RAY +++ + ++ L
Sbjct: 544 ------------ALTYDEKRGACSVGTNVRDAACYVCWAFARAYEPQELKPFVTAISSAL 603
Query: 631 LTVACYDREVNCRRAAAAAFQENVGRQGNYPHGIDIVNTADYFSLASRVTSYMKVAVCIA 690
+ A +DR++NCRRAA+AAFQENVGRQG +PHGIDI+ TADYF++ +R ++ ++V IA
Sbjct: 604 VIAAVFDRDINCRRAASAAFQENVGRQGTFPHGIDILTTADYFAVGNRSNCFLVISVFIA 663
Query: 691 QYEGYLLPFIDELLCNKICHWDKGLRELAADALSALVKYDPEYFASYAVEKLIPCTLSSD 750
+ Y P ID L+ KI HWD +RELAA AL L + PE+ A+ +L+ TLS D
Sbjct: 664 GFPEYTQPMIDHLVTMKISHWDGVIRELAARALHNLAQQAPEFSATQVFPRLLSMTLSPD 723
Query: 751 LCMRHGATLAVGEVVLSLHQCG---------HILSSDIQKRVAGIVPAIEKARLYRGKGG 810
L MRHG+ LA EV +L++ H+ +Q + I + +LYRG GG
Sbjct: 724 LHMRHGSILACAEVAYALYKLAAQENRPVTDHLDEQAVQ-GLKQIHQQLYDRQLYRGLGG 783
Query: 811 EIMRAAVSRFIECISLSHLPLPEKTKRTLLDA----LNENLR-------HPNSQIQNAAV 870
++MR AV IE +SLS +P T++D +N+ LR H Q+++AAV
Sbjct: 784 QLMRQAVCVLIEKLSLSKMPF---RGDTVIDGWQWLINDTLRHLHLISSHSRQQMKDAAV 843
Query: 871 KSLKPFVPAYL----ATADAGKSGNVATKYVEQLSDPNVAVRRGSALALSVLPYEFLANR 930
+L Y AD + T+Y+ +L +P R G +LAL LP L R
Sbjct: 844 SALAALCSEYYMKEPGEADPAIQEELITQYLAELRNPEEMTRCGFSLALGALPGFLLKGR 903
Query: 931 WKDVLLKLCCSCAIEDNPDDRD-AEARVNAVRGLVSVCETLVQGRECSNEDDVPLLYLIK 990
+ VL L +P+D AE+R + ++ + +C+T+ G + D+ +
Sbjct: 904 LQQVLTGL--RAVTHTSPEDVSFAESRRDGLKAIARICQTV--GVKAGAPDEAVCGENV- 963
Query: 991 DEVMASLFKALDDYSVDNRGDVGSWVREAAMNGLEKCTYILCARDSNGFPKKLNGVGSEP 1050
++ +L +DDY+ D+RGDVG+WVR+AAM L T +L S+P
Sbjct: 964 SQIYCALLGCMDDYTTDSRGDVGTWVRKAAMTSLMDLTLLLAR--------------SQP 1023
Query: 1051 ---ESHHCEMAEKDQIDSFFDSTMATSLVGGICKQAVEKLDKLREAAAMVLQRILYNKII 1110
E+H CE ++ + +QA EK+D+ R AA V +L+
Sbjct: 1024 ELIEAHTCE-----------------RIMCCVAQQASEKIDRFRAHAASVFLTLLHFDSP 1083
Query: 1111 HVPHIPFQEILEEIVP--DDPDMKWGVPAITYPRFVRLLQFGCYSKTVMSGLVISIGGMQ 1170
+PH+P + LE++ P D + W P+ +PR +LL Y V+ GLV+S+GG+
Sbjct: 1084 PIPHVPHRGELEKLFPRSDVASVNWSAPSQAFPRITQLLGLPTYRYHVLLGLVVSLGGLT 1143
Query: 1171 DSLSKASMSALMEYLEGDAIGDQDKSSRKGILFTDLLWILQRYKKCDRVIIPTFKTIEIL 1230
+S + S +L EY++G Q S G LL I + +RV +P KT++ +
Sbjct: 1144 ESTIRHSTQSLFEYMKGIQSDPQALGSFSG----TLLQIFEDNLLNERVSVPLLKTLDHV 1189
Query: 1231 FSK---RILLNVEVHISSFCNGILDSMEIELKGSKDFSKLYAGIAILGYIASLTEPVNSR 1290
+ I E H F +L + E+K SKD KL +GIA+ + V +
Sbjct: 1204 LTHGCFDIFTTEEDH--PFAVKLLALCKKEIKNSKDIQKLLSGIAVFCEMVQFPGDVRRQ 1189
Query: 1291 AFFHLLTFLSHRYPKIRKVSAEQVYLVLLQNGDFVPENKIEQALEIVSNTCWEGDLENAK 1340
A L L HR+P IRK +A QVY LL D V + +++ + ++S+T W+ +L +
Sbjct: 1264 ALLQLCLLLCHRFPLIRKTTASQVYETLLTYSDVVGADVLDEVVTVLSDTAWDAELAVVR 1189
BLAST of Spg023281 vs. ExPASy Swiss-Prot
Match:
Q28205 (Tubulin-specific chaperone D OS=Bos taurus OX=9913 GN=TBCD PE=1 SV=1)
HSP 1 Score: 587.4 bits (1513), Expect = 4.1e-166
Identity = 422/1281 (32.94%), Postives = 622/1281 (48.56%), Query Frame = 0
Query: 91 MDKYQEQGQLVEPYLDIIVSPLMSLVCSKAAELGVASDEVLEVIKPICIIIYTLVTVCGY 150
MDKYQEQ L++P+L+ +++ L+ V +K + +D V K +Y + V GY
Sbjct: 72 MDKYQEQPHLLDPHLEWMLNLLLEFVQNKTS----PADLVHLAFK----FLYIISKVRGY 131
Query: 151 KAVIRFFPHQVSDLELAVALLERCQNTNSVTSLRQESTGEMEAQCVILLWLSILVLVPFD 210
K +R FPH+V+D++ + + T+ + E + ++LLWLS+ L+PFD
Sbjct: 132 KTFLRLFPHEVADVQPVLDMF---------TNQNPKDHETWETRYMLLLWLSVTCLIPFD 191
Query: 211 ISTVDSSLSNASSLAKFEPSPLVLRIVSFCKDYLSSAGPMRTMAGLLLARLLTRPDM-PK 270
S +D +LS E + + RI+ + YL + R A +L+++ +TRPD+ K
Sbjct: 192 FSRLDGNLSQPGQ----ERASTMDRILQVAESYLVVSDKARDAAAVLVSKFVTRPDVKQK 251
Query: 271 AFISFTEWTHEALSATTNDVMDH-FRLLGAAGALASIFKTGDRKLLLDVVPQVWN--DTS 330
SF +W+ L+ ++ ++ + G ALA IFK G R+ L V D+
Sbjct: 252 KMASFLDWSLCTLARSSFQTIEGVIAMDGTLQALAQIFKHGKREDCLPYAATVLQCLDSC 311
Query: 331 MLIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHRAASWHYVLLSSSTVDRAKNLVLGVNCV 390
L SN LLRK +KL QR+GLT L + A W Y
Sbjct: 312 RLPDSN----QTLLRKLGVKLVQRLGLTFLKPQVAKWRYQR------------------- 371
Query: 391 LISKCASLVGCESRTSSLGDNISATVSVGTHKCSPINAVELADVCGSSSSLEDEDMEVPE 450
GC S SL +I + P+ E D D +VPE
Sbjct: 372 ---------GCRSLAESLQHSIQ-------NPREPVTQAETPD--------SDGQDDVPE 431
Query: 451 IIEEIIEMLLTGLKDTDTVVRWSAAKGLGRVTSRLTSALSEEVLSSVLELFSPGEGDGSW 510
+E +IE LL GLKD DT+VRWSAAKG+GR+ RL L+++V SVL+ FS E D +W
Sbjct: 432 EVESVIEQLLVGLKDKDTIVRWSAAKGIGRMAGRLPKELADDVTGSVLDCFSFQETDSAW 491
Query: 511 HGGCLALAELARRGLLLPYSLPQVVPIVVKEVEFVIEVEFDNYSKIVDVFKWVFEEEVVM 570
HGGCLALAEL RRGLLLP L VVP++++
Sbjct: 492 HGGCLALAELGRRGLLLPSRLSDVVPVILR------------------------------ 551
Query: 571 VKTVVEDVDMEVALHYDIRRGPHSVGSHVRDAAAYVCWAFGRAYHHTDMREILKQLAPHL 630
AL Y+ +RG SVGS+VRDAA YVCWAF RAY +++ + ++ L
Sbjct: 552 ------------ALTYEEKRGACSVGSNVRDAACYVCWAFARAYEPQELKPFVAAISSAL 611
Query: 631 LTVACYDREVNCRRAAAAAFQENVGRQGNYPHGIDIVNTADYFSLASRVTSYMKVAVCIA 690
+ +DR+VNCRRAA+AAFQENVGRQG +PHGIDI+ TADYF++ +R ++ +++ IA
Sbjct: 612 VIATVFDRDVNCRRAASAAFQENVGRQGTFPHGIDILTTADYFAVGNRSNCFLVISMFIA 671
Query: 691 QYEGYLLPFIDELLCNKICHWDKGLRELAADALSALVKYDPEYFASYAVEKLIPCTLSSD 750
+ Y P I+ L+ K+ HWD +REL+A AL L + PE+ A +L+ T S D
Sbjct: 672 GFPEYTQPMIEHLVTMKVGHWDGTIRELSAKALRNLAQRAPEHTAREVFPRLLSMTQSPD 731
Query: 751 LCMRHGATLAVGEVVLSLH----QCGHILSSDIQKRVAGIVPAIEK----ARLYRGKGGE 810
L RHGA LA EV SLH Q G +S + ++ + I + +LYRG GGE
Sbjct: 732 LHTRHGAVLACAEVARSLHTLATQQGRPVSDFLDEKAMHGLKQIHQQLYDRQLYRGLGGE 791
Query: 811 IMRAAVSRFIECISLSHLPLPEKTK----RTLLDALNENLR----HPNSQIQNAAVKSLK 870
+MR AV IE ++LS +P + L++ +NL H I+ AAV +L
Sbjct: 792 LMRQAVCILIENVALSKMPFRGDAVIDGWQWLINDTLKNLHLISSHSRQHIKEAAVSALA 851
Query: 871 PFVPAYLA----TADAGKSGNVATKYVEQLSDPNVAVRRGSALALSVLPYEFLANRWKDV 930
Y A A+A + Y+ +L P R G ALAL LP FL R + V
Sbjct: 852 ALCSEYHAQEPGEAEAAAQEELVKLYLAELQSPEEMTRCGCALALGALPAFFLKGRLRQV 911
Query: 931 LLKLCCSCAIEDNPDDRDAEARVNAVRGLVSVCETLVQGRECSNEDDVPLLYLIKDEVMA 990
L L I D AEAR +A++ + +C+T+ G D + ++
Sbjct: 912 LAGLRAVTHISPK-DVSFAEARRDALKAISRICQTV--GVRAEGPPDEAVCRENVSQIYC 971
Query: 991 SLFKALDDYSVDNRGDVGSWVREAAMNGLEKCTYILCARDSNGFPKKLNGVGSEPESHHC 1050
+L L DY+ D+RGDVG+WVREAAM L T +L ++PE
Sbjct: 972 TLLDCLKDYTTDSRGDVGAWVREAAMTSLMDLTLLLGR--------------NQPE---- 1031
Query: 1051 EMAEKDQIDSFFDSTMATSLVGGICKQAVEKLDKLREAAAMVLQRILYNKIIHVPHIPFQ 1110
++ + L+ + +QA EK+D+ R AA V +L+ +PH+P +
Sbjct: 1032 ----------LIEAPLCQQLMCCLAQQASEKIDRFRAHAARVFLALLHADSPAIPHVPAR 1091
Query: 1111 EILEEIVPDD--PDMKWGVPAITYPRFVRLLQFGCYSKTVMSGLVISIGGMQDSLSKASM 1170
LE + P + WG P+ +PR RLL Y V+ GL +S+GG+ +S + S
Sbjct: 1092 PELERLFPRAAVASVNWGAPSQAFPRMARLLGLPAYRYHVLLGLAVSVGGLTESTVRYST 1151
Query: 1171 SALMEY---LEGDAIGDQDKSSRKGILFTDLLWILQRYKKCDRVIIPTFKTIEILFSK-- 1230
L EY ++ D +D LL + + DRV +P KT++ + +
Sbjct: 1152 QGLFEYMKEIQNDPAALEDFGG-------TLLQVFEDNLLNDRVSVPLLKTLDQMLANGC 1196
Query: 1231 -RILLNVEVHISSFCNGILDSMEIELKGSKDFSKLYAGIAILGYIASLTEPVNSRAFFHL 1290
I E H FC +L + E+K SKD KL + IA+ + V + L
Sbjct: 1212 FDIFTAQENH--PFCVKLLALCKEEIKKSKDVQKLRSSIAVFCGLVQFPGDVRRKVLLQL 1196
Query: 1291 LTFLSHRYPKIRKVSAEQVYLVLLQNGDFVPENKIEQALEIVSNTCWEGDLENAKLQRRE 1340
L H +P IRK +A QVY ++L D VP +++ + ++S+T W+ +L + QR
Sbjct: 1272 FLLLCHPFPVIRKNTASQVYEMVL-TYDVVPTAVLDEVMAVLSSTAWDAELPVVRAQRNR 1196
BLAST of Spg023281 vs. ExPASy Swiss-Prot
Match:
Q8BYA0 (Tubulin-specific chaperone D OS=Mus musculus OX=10090 GN=Tbcd PE=1 SV=1)
HSP 1 Score: 578.2 bits (1489), Expect = 2.5e-163
Identity = 420/1304 (32.21%), Postives = 633/1304 (48.54%), Query Frame = 0
Query: 70 MQASHSEVVRISSRDAYWAPFMDKYQEQGQLVEPYLDIIVSPLMSLVCSKAAELGVASDE 129
+ A H E + + MDKYQEQ L++P+L+ +++ L+ LV + +
Sbjct: 43 LPAVHRERASREVAEERFRVIMDKYQEQPHLLDPHLEWMMNSLLDLVQDETS-------- 102
Query: 130 VLEVIKPICIIIYTLVTVCGYKAVIRFFPHQVSDLELAVALLERCQNTNSVTSLRQESTG 189
+ +++ +Y + V GYK +R FPH+V++++ + + T +
Sbjct: 103 LPDLVHLAFKFLYIITKVRGYKVFLRLFPHEVANVQPVLDMF---------TGQNPKDHE 162
Query: 190 EMEAQCVILLWLSILVLVPFDISTVDSSLSNASSLAKFEPSPLVLRIVSFCKDYLSSAGP 249
E + ++LLWLS+ L+PFD S +D +LS + + P + RI+ + YL +
Sbjct: 163 TWETRYMLLLWLSVTCLIPFDFSRLDGNLSTQTGETRV---PTMDRILQIAESYLVVSDK 222
Query: 250 MRTMAGLLLARLLTRPDM-PKAFISFTEWTHEALSATTNDVMDH-FRLLGAAGALASIFK 309
R A +L+++ +TRPD+ + SF +W+ L+ ++ ++ + G ALA IFK
Sbjct: 223 ARDAAAVLVSKFITRPDVKQRKMASFLDWSLCTLAHSSFQTIEGVITMDGMLQALAQIFK 282
Query: 310 TGDRKLLL---DVVPQVWNDTSMLIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHRAASWH 369
G R+ L + V Q + + S+T+ LRK +KL QR+GLT L + A+W
Sbjct: 283 HGKREDCLPYANTVLQCLDGCRLPESSHTS-----LRKLGVKLVQRLGLTFLKPKVATWR 342
Query: 370 YVLLSSSTVDRAKNLVLGVNCVLISKCASLVGCESRTSSLGDNISATVSVGTHKCSPINA 429
Y GC S ++L C+P +
Sbjct: 343 YQR----------------------------GCRSLAANL------------KLCAPGKS 402
Query: 430 VELADVCGSSSSLEDEDMEVPEIIEEIIEMLLTGLKDTDTVVRWSAAKGLGRVTSRLTSA 489
+ + S +S DED +VPE +E +IE LL GLKD DTVVRWSAAKG+GR+ RL
Sbjct: 403 DQKL-LSDSLTSDGDEDYDVPEGVETVIEQLLVGLKDKDTVVRWSAAKGIGRMAGRLPRE 462
Query: 490 LSEEVLSSVLELFSPGEGDGSWHGGCLALAELARRGLLLPYSLPQVVPIVVKEVEFVIEV 549
L+++V+ SVL+ FS E D +WHGGCLALAEL RRGLLLP L +VV +++K
Sbjct: 463 LADDVVGSVLDCFSFQETDKAWHGGCLALAELGRRGLLLPSRLSEVVTVILK-------- 522
Query: 550 EFDNYSKIVDVFKWVFEEEVVMVKTVVEDVDMEVALHYDIRRGPHSVGSHVRDAAAYVCW 609
AL YD +RG SVG++VRDAA YVCW
Sbjct: 523 ----------------------------------ALTYDEKRGACSVGANVRDAACYVCW 582
Query: 610 AFGRAYHHTDMREILKQLAPHLLTVACYDREVNCRRAAAAAFQENVGRQGNYPHGIDIVN 669
AF RAY ++ + ++ L+ A +DR VNCRRAA+AAFQENVGRQG +PHGIDI+
Sbjct: 583 AFARAYEPQELTPFVTAISSALVIAAVFDRNVNCRRAASAAFQENVGRQGTFPHGIDILT 642
Query: 670 TADYFSLASRVTSYMKVAVCIAQYEGYLLPFIDELLCNKICHWDKGLRELAADALSALVK 729
TADYF++ + ++ ++V IA ++ Y P ID L+ KI HWD +REL+A AL L
Sbjct: 643 TADYFAVGNISNCFLIISVFIAGFQEYTKPMIDHLVSMKINHWDGAIRELSAKALHNLTP 702
Query: 730 YDPEYFASYAVEKLIPCTLSSDLCMRHGATLAVGEVVLSLH----QCGHILSSDIQKRVA 789
PEY A + L+ T S DL RHGA LA EV +L+ Q +++ + ++
Sbjct: 703 QVPEYIAMHVFPALLLMTQSPDLHTRHGAILACAEVTYALYKLATQSNRLVTDYLDEKAV 762
Query: 790 GIVPAIEK----ARLYRGKGGEIMRAAVSRFIECISLSHLPLP-EKTKRTLLDALNENLR 849
+ I + LYRG GGE+MR AV IE +SLS +P + T +N+ LR
Sbjct: 763 QSLKQIHQQLCDRHLYRGLGGELMRQAVCILIEKLSLSRMPFKGDATVEGWQWLINDTLR 822
Query: 850 -------HPNSQIQNAAVKSLKPFVPAYLATADAGKSGNVATK-----YVEQLSDPNVAV 909
H QI+ AV +L Y + G++G+ K Y+ +L P
Sbjct: 823 SLHLVSSHSRQQIKEVAVSALTALCSEYY-VKEPGEAGSSIAKELIPQYLAELQSPEEMA 882
Query: 910 RRGSALALSVLPYEFLANRWKDVLLKLCCSCAIEDNPDDRDAEARVNAVRGLVSVCETLV 969
R G + AL LP L + VL L I N D AEAR + ++ + +C+T+
Sbjct: 883 RCGFSSALGALPGFLLRGHLQQVLSGLRRVTCISPN-DVSFAEARRDGLKAISRICQTV- 942
Query: 970 QGRECSNEDDVPLLYLIKDEVMASLFKALDDYSVDNRGDVGSWVREAAMNGLEKCTYILC 1029
G D + EV A+L + DY+ D+RGDVG+WVREAAM L +L
Sbjct: 943 -GVNTRGPPDEVICKENISEVYAALLGCMSDYTTDSRGDVGAWVREAAMTSLMDLMLLLA 1002
Query: 1030 ARDSNGFPKKLNGVGSEP---ESHHCEMAEKDQIDSFFDSTMATSLVGGICKQAVEKLDK 1089
+EP E+H CE ++ + +QA EK+D+
Sbjct: 1003 R--------------TEPVLIEAHICE-----------------RVMCCVAQQASEKIDR 1062
Query: 1090 LREAAAMVLQRILYNKIIHVPHIPFQEILEEIVP--DDPDMKWGVPAITYPRFVRLLQFG 1149
R AA V +L+ +PH+P ++ LE + P D + W P+ +P +LL
Sbjct: 1063 FRAHAARVFLTLLHFDSPPIPHVPHRQELESLFPRSDVATVNWNAPSQAFPLITQLLGLP 1122
Query: 1150 CYSKTVMSGLVISIGGMQDSLSKASMSALMEYLEGDAIGDQDKSSRKGILFTDLLWILQR 1209
Y V+ GL +S+GG+ +S + S +L EY++G Q S LL + +
Sbjct: 1123 TYRYHVLLGLAVSVGGLTESTVRHSTQSLFEYMKGIQKDAQVLQS----FSETLLKVFED 1182
Query: 1210 YKKCDRVIIPTFKTIEILFSK---RILLNVEVHISSFCNGILDSMEIELKGSKDFSKLYA 1269
DRV + K ++ L + I E H FC +L + E+K SKD KL +
Sbjct: 1183 NLLNDRVSVSLLKMLDQLLANGCFDIFTAEENH--PFCVKLLTLCKEEIKKSKDIQKLRS 1192
Query: 1270 GIAILGYIASLTEPVNSRAFFHLLTFLSHRYPKIRKVSAEQVYLVLLQNGDFVPENKIEQ 1329
IA+L + V + L L H +P IRK +A QVY ++L D V +++
Sbjct: 1243 SIAVLCGMVQFNGDVRKKILLQLFLLLGHPFPVIRKSTASQVYEMVLTYSDLVDAEVLDE 1192
Query: 1330 ALEIVSNTCWEGDLENAKLQRRELSDIAGIETDLRPKTNTVPPP 1340
+ ++S+T W+ +L + QR L D+ G+ P+ VP P
Sbjct: 1303 VMSVLSDTAWDAELPVVREQRNRLCDLLGV-----PRPQLVPKP 1192
BLAST of Spg023281 vs. ExPASy Swiss-Prot
Match:
Q5ZI87 (Tubulin-specific chaperone D OS=Gallus gallus OX=9031 GN=TBCD PE=2 SV=1)
HSP 1 Score: 510.0 bits (1312), Expect = 8.4e-143
Identity = 354/1077 (32.87%), Postives = 531/1077 (49.30%), Query Frame = 0
Query: 91 MDKYQEQGQLVEPYLDIIVSPLMSLVCSKAAELGVASDEVLEVIKPICIIIYTLVTVCGY 150
MDKYQEQ L++ +L+ +++ L+ ++ S VL +Y + V GY
Sbjct: 65 MDKYQEQPHLLDRHLEWMMNMLLDIIRDS------GSPPVL--FHLAFKFLYIITKVRGY 124
Query: 151 KAVIRFFPHQVSDLELAVALLERCQNTNSVTSLRQESTGEMEAQCVILLWLSILVLVPFD 210
K +R FPH+V+DL+ + + + + E + ++LLWLS++ L+PFD
Sbjct: 125 KLFLRLFPHEVTDLQPVL---------DMIVDQNPKDCETWETRYMLLLWLSMICLIPFD 184
Query: 211 ISTVDSSLSNASSLAKFEPSPLVLRIVSFCKDYLSSAGPMRTMAGLLLARLLTRPDM-PK 270
++ D ++ + + P + RI+ K YL + R A +L+++ + RPD+ K
Sbjct: 185 LARFDGNILSEEGHTRM---PTMDRILEIAKCYLVVSDKARDAAAVLVSKFIVRPDVRQK 244
Query: 271 AFISFTEWTHEALSATTNDVMDHFRLL-GAAGALASIFKTGDRKLLLDVVPQVWN--DTS 330
F +WT LS ++ M+ ++ G ALA +FK G R+ L V D
Sbjct: 245 RMADFLDWTLSMLSKSSFQSMEGTVVMNGMLQALAQLFKHGKREDCLPYAATVLECLDNC 304
Query: 331 MLIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHRAASWHYVLLSSSTVDRAKNLVLGVNCV 390
L +SN V LRK +KL QR+GLT + + A W Y
Sbjct: 305 KLSESNQMV----LRKLGMKLVQRLGLTFVKPKVAKWRYQR------------------- 364
Query: 391 LISKCASLVGCESRTSSLGDNISATVSVGTHKCSPINAVELADVCGSSSSLEDEDMEVPE 450
GC SL N+ A SV + + A E D DE+ ++P
Sbjct: 365 ---------GCR----SLAANLQAQSSVMQSQKITVAANEAED---------DEEYDIPG 424
Query: 451 IIEEIIEMLLTGLKDTDTVVRWSAAKGLGRVTSRLTSALSEEVLSSVLELFSPGEGDGSW 510
IE ++E LL GLKD DT+VRWSAAKG+GR+T RL L+++V+ S+L+ FS E D +W
Sbjct: 425 EIENVVEQLLVGLKDKDTIVRWSAAKGIGRITGRLPKELADDVVGSLLDCFSFQETDNAW 484
Query: 511 HGGCLALAELARRGLLLPYSLPQVVPIVVKEVEFVIEVEFDNYSKIVDVFKWVFEEEVVM 570
HGGCLALAEL RRGLLLP + VVP+++K
Sbjct: 485 HGGCLALAELGRRGLLLPSRISDVVPVILK------------------------------ 544
Query: 571 VKTVVEDVDMEVALHYDIRRGPHSVGSHVRDAAAYVCWAFGRAYHHTDMREILKQLAPHL 630
AL YD +RG SVGS++RDAA Y+ WAF RAY +++ + Q++ L
Sbjct: 545 ------------ALTYDEKRGSCSVGSNLRDAACYLSWAFARAYDPSELIPFINQISSAL 604
Query: 631 LTVACYDREVNCRRAAAAAFQENVGRQGNYPHGIDIVNTADYFSLASRVTSYMKVAVCIA 690
+ A +DR+VNCRRAA+AAFQENVGRQG +PHGIDI+ ADYF++ +RV Y+ ++V IA
Sbjct: 605 VIAAVFDRDVNCRRAASAAFQENVGRQGTFPHGIDILTAADYFAVGNRVNCYLTISVYIA 664
Query: 691 QYEGYLLPFIDELLCNKICHWDKGLRELAADALSALVKYDPEYFASYAVEKLIPCTLSSD 750
+ Y P ID L+ KI HWD +REL+ AL L PEY A+ + +L+P ++ +D
Sbjct: 665 GFPEYTQPMIDHLVNMKINHWDSVIRELSTKALHNLTPRAPEYMANVVLPRLLPLSVGTD 724
Query: 751 LCMRHGATLAVGEVVLSLHQCGHILSSDIQ-----KRVAGIVPAIEK---ARLYRGKGGE 810
L RHGA LA E+ +L + + I K + G+ ++ +LYRG GGE
Sbjct: 725 LHTRHGAILACAEITHALCKLAEENNRSITYYFNGKSLEGLKQIHQELCSRQLYRGLGGE 784
Query: 811 IMRAAVSRFIECISLSHLPLP-EKTKRTLLDALNENLRH-------PNSQIQNAAVKSLK 870
+MR AV IE +SLS +P + +N++LR ++ +AV +L
Sbjct: 785 LMRPAVCTLIEKLSLSKMPFKGDPIIEGWQWLINDSLRSLPLASCAARQHVKESAVSALS 844
Query: 871 PFVPAYL----ATADAGKSGNVATKYVEQLSDPNVAVRRGSALALSVLPYEFLANRWKDV 930
Y AD G + T+Y+ +L +R G +LAL LP L R + V
Sbjct: 845 ALCNEYYINENGEADPALQGELVTQYISELQSTEQMIRCGFSLALGALPRFLLKGRLQQV 904
Query: 931 LLKLCCSCAIEDNPDDRDAEARVNAVRGLVSVCETLVQGRECSNEDDVPLLYLIKD---E 990
L L I D AE+R +A+ + +C+T+ E S E+ Y+ KD +
Sbjct: 905 LEGLRKVTLITPR-DVSFAESRRDALIAIAEICQTVGVKGEGSQEE-----YICKDNVAQ 964
Query: 991 VMASLFKALDDYSVDNRGDVGSWVREAAMNGLEKCTYILCARDSNGFPKKLNGVGSEPES 1050
+ A+L + DY+ D+RGDVG WVREAAM L K T +L ++
Sbjct: 965 IYATLLNCVTDYTTDSRGDVGGWVREAAMTSLMKVTLLLVQNEA---------------- 1000
Query: 1051 HHCEMAEKDQIDSFFDSTMATSLVGGICKQAVEKLDKLREAAAMVLQRILYNKIIHVPHI 1110
++ + ++ + +Q+ EK+DK R A V +L+ VPHI
Sbjct: 1025 ------------ELINANICKQIMCWLAQQSAEKIDKFRAHAGSVFLTLLHFDSPPVPHI 1000
Query: 1111 PFQEILEEIVP--DDPDMKWGVPAITYPRFVRLLQFGCYSKTVMSGLVISIGGMQDS 1139
P +E LE I P + + W + +PR +LL Y V+ GL +S+GG+ ++
Sbjct: 1085 PHREELERIFPRSEAETLNWNAASEAFPRITQLLALPAYQYYVLLGLSVSVGGLTET 1000
BLAST of Spg023281 vs. ExPASy TrEMBL
Match:
A0A6J1KEP9 (Tubulin-specific chaperone D OS=Cucurbita maxima OX=3661 GN=LOC111494326 PE=3 SV=1)
HSP 1 Score: 2275.4 bits (5895), Expect = 0.0e+00
Identity = 1174/1365 (86.01%), Postives = 1224/1365 (89.67%), Query Frame = 0
Query: 1 MAATTETAPKKEEVDGLITYDDDDEHESKERVIQKYFLQEWKLVKSILDDIVSNGCVSDI 60
MAATTETAPK EE DG++TYDDDDEHESKERVIQKYFLQEWKLVKSILDDI SNG VSDI
Sbjct: 1 MAATTETAPKNEEFDGMVTYDDDDEHESKERVIQKYFLQEWKLVKSILDDIASNGRVSDI 60
Query: 61 SAVHKIRSIMQASHSEVVRISSRDAYWAPFMDKYQEQGQLVEPYLDIIVSPLMSLVCSKA 120
S+VHKIRSI MDKYQEQGQLVEPYL+IIVSPLMSLVCSKA
Sbjct: 61 SSVHKIRSI---------------------MDKYQEQGQLVEPYLEIIVSPLMSLVCSKA 120
Query: 121 AELGVASDEVLEVIKPICIIIYTLVTVCGYKAVIRFFPHQVSDLELAVALLERCQNTNSV 180
EL VAS++VLE IKPICIIIYTLVTVCGYKAVIRFFPHQVSDLELAV+LLERCQNTNSV
Sbjct: 121 TELDVASNKVLEAIKPICIIIYTLVTVCGYKAVIRFFPHQVSDLELAVSLLERCQNTNSV 180
Query: 181 TSLRQESTGEMEAQCVILLWLSILVLVPFDISTVDSSLSNASSLAKFEPSPLVLRIVSFC 240
T+LRQESTGEMEAQCVILLWLSILVLVPFDISTVDSSLS+ +SLAKFEPSPLVLRI+SFC
Sbjct: 181 TTLRQESTGEMEAQCVILLWLSILVLVPFDISTVDSSLSSTNSLAKFEPSPLVLRIISFC 240
Query: 241 KDYLSSAGPMRTMAGLLLARLLTRPDMPKAFISFTEWTHEALSATTNDVMDHFRLLGAAG 300
KDYLSSAGPMRTMAGLLLARLLTRPDMPKAFISF EWTHEALSATTND+MDHFRLLGAAG
Sbjct: 241 KDYLSSAGPMRTMAGLLLARLLTRPDMPKAFISFIEWTHEALSATTNDIMDHFRLLGAAG 300
Query: 301 ALASIFKTGDRKLLLDVVPQVWNDTSMLIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHRA 360
ALASIFKTGDRKLLLDVVPQVWNDTS LIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHRA
Sbjct: 301 ALASIFKTGDRKLLLDVVPQVWNDTSTLIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHRA 360
Query: 361 ASWHYVLLSSSTVDRAKNLVLGVNCVLISKCASLVGCESRTSSLGDNISATVSVGTHKCS 420
ASWHYV SRTSSLGDNISA VSVGTH C+
Sbjct: 361 ASWHYV--------------------------------SRTSSLGDNISAPVSVGTHTCT 420
Query: 421 PINAVELADVCGSSSSLEDEDMEVPEIIEEIIEMLLTGLKDTDTVVRWSAAKGLGRVTSR 480
PINAV+L+DVC SS+SLEDEDMEVPEIIEEIIE LLTGLKDTDTVVRWSAAKGLGRVTSR
Sbjct: 421 PINAVKLSDVCQSSTSLEDEDMEVPEIIEEIIETLLTGLKDTDTVVRWSAAKGLGRVTSR 480
Query: 481 LTSALSEEVLSSVLELFSPGEGDGSWHGGCLALAELARRGLLLPYSLPQVVPIVVKEVEF 540
LTSALSEEVLSS+LELFSPGEGDGSWHGGCLALAELARRGLLLP SLPQVVPIVVK
Sbjct: 481 LTSALSEEVLSSILELFSPGEGDGSWHGGCLALAELARRGLLLPLSLPQVVPIVVK---- 540
Query: 541 VIEVEFDNYSKIVDVFKWVFEEEVVMVKTVVEDVDMEVALHYDIRRGPHSVGSHVRDAAA 600
ALHYDIRRGPHSVGSHVRDAAA
Sbjct: 541 --------------------------------------ALHYDIRRGPHSVGSHVRDAAA 600
Query: 601 YVCWAFGRAYHHTDMREILKQLAPHLLTVACYDREVNCRRAAAAAFQENVGRQGNYPHGI 660
YVCWAFGRAYHHTDMR+ILKQLAPHLLTVACYDREVNCRRAAAAAFQENVGRQGNYPHGI
Sbjct: 601 YVCWAFGRAYHHTDMRDILKQLAPHLLTVACYDREVNCRRAAAAAFQENVGRQGNYPHGI 660
Query: 661 DIVNTADYFSLASRVTSYMKVAVCIAQYEGYLLPFIDELLCNKICHWDKGLRELAADALS 720
DIVNTADYFSLASRVTSY+KVAVCI+QYEG+LLPFIDELLCNKICHWDKGLRELAADALS
Sbjct: 661 DIVNTADYFSLASRVTSYLKVAVCISQYEGFLLPFIDELLCNKICHWDKGLRELAADALS 720
Query: 721 ALVKYDPEYFASYAVEKLIPCTLSSDLCMRHGATLAVGEVVLSLHQCGHILSSDIQKRVA 780
ALVKYDPEYFASYAVEKLIPCTLSSDLCMRHGATLAVGEVVLSLHQCGHILSSDIQKRVA
Sbjct: 721 ALVKYDPEYFASYAVEKLIPCTLSSDLCMRHGATLAVGEVVLSLHQCGHILSSDIQKRVA 780
Query: 781 GIVPAIEKARLYRGKGGEIMRAAVSRFIECISLSHLPLPEKTKRTLLDALNENLRHPNSQ 840
GIVPAIEKARLYRGKGGEIMRAAVSRFIECISLSHLPL EKTKR LLDALNENLRHPNSQ
Sbjct: 781 GIVPAIEKARLYRGKGGEIMRAAVSRFIECISLSHLPLLEKTKRMLLDALNENLRHPNSQ 840
Query: 841 IQNAAVKSLKPFVPAYLATADAGKSGNVATKYVEQLSDPNVAVRRGSALALSVLPYEFLA 900
IQNAAVKSLKPFV AYLATADAGKSGN+ TKY+EQLSDPNVAVRRGSALALSVLPYEFLA
Sbjct: 841 IQNAAVKSLKPFVLAYLATADAGKSGNITTKYLEQLSDPNVAVRRGSALALSVLPYEFLA 900
Query: 901 NRWKDVLLKLCCSCAIEDNPDDRDAEARVNAVRGLVSVCETLVQGRECSNEDDVPLLYLI 960
NRWKDVLLKLCCSCAIEDNPDDRDAEARVNAVRGLVSVCETLVQGRE SN D++PLLYLI
Sbjct: 901 NRWKDVLLKLCCSCAIEDNPDDRDAEARVNAVRGLVSVCETLVQGREFSNVDELPLLYLI 960
Query: 961 KDEVMASLFKALDDYSVDNRGDVGSWVREAAMNGLEKCTYILCARDSNGFPKKLNGVGSE 1020
KDEVMASLFKALDDYSVDNRGDVGSWVREAAM GLEKCTYILCARDSNGF KKLN VGSE
Sbjct: 961 KDEVMASLFKALDDYSVDNRGDVGSWVREAAMIGLEKCTYILCARDSNGFTKKLNSVGSE 1020
Query: 1021 PESHHCEMAEKDQIDSFFDSTMATSLVGGICKQAVEKLDKLREAAAMVLQRILYNKIIHV 1080
ES HCEM+EKDQ +SFFDS MATSLVGGICKQAVEKLDKLREAAAMVL RILY+K+I V
Sbjct: 1021 QESLHCEMSEKDQTNSFFDSAMATSLVGGICKQAVEKLDKLREAAAMVLLRILYSKVILV 1080
Query: 1081 PHIPFQEILEEIVPDDPDMKWGVPAITYPRFVRLLQFGCYSKTVMSGLVISIGGMQDSLS 1140
PHIPF++ LEEI+PDDP+MKW VPA++YP FVRLLQFGCYSKTVMSGLVISIGGMQDSLS
Sbjct: 1081 PHIPFRDTLEEIIPDDPNMKWAVPAVSYPHFVRLLQFGCYSKTVMSGLVISIGGMQDSLS 1140
Query: 1141 KASMSALMEYLEGDAIGDQDKSSRKGILFTDLLWILQRYKKCDRVIIPTFKTIEILFSKR 1200
KAS+SALMEYLEGDAI DQDK SRKG+LFTDL+WILQRYKKCDRVIIPTFKTIEILFSKR
Sbjct: 1141 KASISALMEYLEGDAIEDQDKRSRKGMLFTDLIWILQRYKKCDRVIIPTFKTIEILFSKR 1200
Query: 1201 ILLNVEVHISSFCNGILDSMEIELKGSKDFSKLYAGIAILGYIASLTEPVNSRAFFHLLT 1260
ILLN+EVH+SSFCNGILDS+EIE+KGSKDFSKLYAGI+ILGYI SL+EPVNSRAF HLL+
Sbjct: 1201 ILLNMEVHLSSFCNGILDSLEIEMKGSKDFSKLYAGISILGYITSLSEPVNSRAFVHLLS 1260
Query: 1261 FLSHRYPKIRKVSAEQVYLVLLQNGDFVPENKIEQALEIVSNTCWEGDLENAKLQRRELS 1320
FLSHRYPKIRK SAEQVYLVLLQNGDFVPE+KIEQALEIVSNTCWEGDLENAKLQR+ELS
Sbjct: 1261 FLSHRYPKIRKASAEQVYLVLLQNGDFVPEDKIEQALEIVSNTCWEGDLENAKLQRQELS 1266
Query: 1321 DIAGIETDLRPKTNTVPPPEKEVKNRFSAADENASYSSLVESTGF 1366
DIAGIE +L P V PEKEVKNRFSA+DENA+YSSLVESTGF
Sbjct: 1321 DIAGIEINLHP----VRAPEKEVKNRFSASDENATYSSLVESTGF 1266
BLAST of Spg023281 vs. ExPASy TrEMBL
Match:
A0A6J1HHJ4 (Tubulin-specific chaperone D OS=Cucurbita moschata OX=3662 GN=LOC111464377 PE=3 SV=1)
HSP 1 Score: 2275.0 bits (5894), Expect = 0.0e+00
Identity = 1174/1365 (86.01%), Postives = 1222/1365 (89.52%), Query Frame = 0
Query: 1 MAATTETAPKKEEVDGLITYDDDDEHESKERVIQKYFLQEWKLVKSILDDIVSNGCVSDI 60
MAA TETAPK EE DG++TYDDDDEHESKERVIQKYFLQEWKLVKSILDDI SNG VSDI
Sbjct: 1 MAAKTETAPKNEEFDGMVTYDDDDEHESKERVIQKYFLQEWKLVKSILDDIASNGRVSDI 60
Query: 61 SAVHKIRSIMQASHSEVVRISSRDAYWAPFMDKYQEQGQLVEPYLDIIVSPLMSLVCSKA 120
S+VHKIRSI MDKYQEQGQLVEPYL+IIVSPLMSLVCSKA
Sbjct: 61 SSVHKIRSI---------------------MDKYQEQGQLVEPYLEIIVSPLMSLVCSKA 120
Query: 121 AELGVASDEVLEVIKPICIIIYTLVTVCGYKAVIRFFPHQVSDLELAVALLERCQNTNSV 180
EL VAS+EVLE IKPICIIIYTLVTVCGYKAVIRFFPHQVSDLELAV+LLERCQNTNSV
Sbjct: 121 IELDVASNEVLEAIKPICIIIYTLVTVCGYKAVIRFFPHQVSDLELAVSLLERCQNTNSV 180
Query: 181 TSLRQESTGEMEAQCVILLWLSILVLVPFDISTVDSSLSNASSLAKFEPSPLVLRIVSFC 240
T+LRQESTGEMEAQCVILLWLSILVLVPFDISTVDSSLS+ +SLAKFEPSPLVLRI+SFC
Sbjct: 181 TTLRQESTGEMEAQCVILLWLSILVLVPFDISTVDSSLSSTNSLAKFEPSPLVLRIISFC 240
Query: 241 KDYLSSAGPMRTMAGLLLARLLTRPDMPKAFISFTEWTHEALSATTNDVMDHFRLLGAAG 300
KDYL+SAGPMRTMAGLLLARLLTRPDMPKAFISF EWTHEALSA TND+MDHFRLLGAAG
Sbjct: 241 KDYLASAGPMRTMAGLLLARLLTRPDMPKAFISFIEWTHEALSAATNDIMDHFRLLGAAG 300
Query: 301 ALASIFKTGDRKLLLDVVPQVWNDTSMLIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHRA 360
ALASIFKTGDRKLLLDVVPQVWNDTS LIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHRA
Sbjct: 301 ALASIFKTGDRKLLLDVVPQVWNDTSTLIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHRA 360
Query: 361 ASWHYVLLSSSTVDRAKNLVLGVNCVLISKCASLVGCESRTSSLGDNISATVSVGTHKCS 420
ASWHYV SRTSSLGDNISA VSVGTHKC+
Sbjct: 361 ASWHYV--------------------------------SRTSSLGDNISAPVSVGTHKCT 420
Query: 421 PINAVELADVCGSSSSLEDEDMEVPEIIEEIIEMLLTGLKDTDTVVRWSAAKGLGRVTSR 480
PINAVEL+DVC SS+SLEDEDMEVPEIIEEIIE LLTGLKDTDTVVRWSAAKGLGRVTSR
Sbjct: 421 PINAVELSDVCQSSTSLEDEDMEVPEIIEEIIETLLTGLKDTDTVVRWSAAKGLGRVTSR 480
Query: 481 LTSALSEEVLSSVLELFSPGEGDGSWHGGCLALAELARRGLLLPYSLPQVVPIVVKEVEF 540
LTSALSEEVLSS+LELFSPGEGDGSWHGGCLALAELARRGLLLP+SLPQVVPIVVK
Sbjct: 481 LTSALSEEVLSSILELFSPGEGDGSWHGGCLALAELARRGLLLPHSLPQVVPIVVK---- 540
Query: 541 VIEVEFDNYSKIVDVFKWVFEEEVVMVKTVVEDVDMEVALHYDIRRGPHSVGSHVRDAAA 600
ALHYDIRRGPHSVGSHVRDAAA
Sbjct: 541 --------------------------------------ALHYDIRRGPHSVGSHVRDAAA 600
Query: 601 YVCWAFGRAYHHTDMREILKQLAPHLLTVACYDREVNCRRAAAAAFQENVGRQGNYPHGI 660
YVCWAFGRAYHHTDMREILKQLAPHLLTVACYDREVNCRRAAAAAFQENVGRQGNYPHGI
Sbjct: 601 YVCWAFGRAYHHTDMREILKQLAPHLLTVACYDREVNCRRAAAAAFQENVGRQGNYPHGI 660
Query: 661 DIVNTADYFSLASRVTSYMKVAVCIAQYEGYLLPFIDELLCNKICHWDKGLRELAADALS 720
DIVNTADYFSLASRVTSY+KVAVCI+QYEG+LLPFIDELLCNKICHWDKGLRELAADALS
Sbjct: 661 DIVNTADYFSLASRVTSYLKVAVCISQYEGFLLPFIDELLCNKICHWDKGLRELAADALS 720
Query: 721 ALVKYDPEYFASYAVEKLIPCTLSSDLCMRHGATLAVGEVVLSLHQCGHILSSDIQKRVA 780
ALVKYDPEYFASYAVEKLIPCTLSSDLCMRHGATLAVGEVVLSLHQCGHILSSDIQKRVA
Sbjct: 721 ALVKYDPEYFASYAVEKLIPCTLSSDLCMRHGATLAVGEVVLSLHQCGHILSSDIQKRVA 780
Query: 781 GIVPAIEKARLYRGKGGEIMRAAVSRFIECISLSHLPLPEKTKRTLLDALNENLRHPNSQ 840
GIVPAIEKARLYRGKGGEIMRAAVSRFIECISLSHLPL EKTKR LLDALNENLRHPNSQ
Sbjct: 781 GIVPAIEKARLYRGKGGEIMRAAVSRFIECISLSHLPLLEKTKRMLLDALNENLRHPNSQ 840
Query: 841 IQNAAVKSLKPFVPAYLATADAGKSGNVATKYVEQLSDPNVAVRRGSALALSVLPYEFLA 900
IQNAAVKSLKPFV AYLATADAGKSGN+ TKY+EQLSDPNVA+RRGSALALSVLPYEFLA
Sbjct: 841 IQNAAVKSLKPFVLAYLATADAGKSGNITTKYLEQLSDPNVAIRRGSALALSVLPYEFLA 900
Query: 901 NRWKDVLLKLCCSCAIEDNPDDRDAEARVNAVRGLVSVCETLVQGRECSNEDDVPLLYLI 960
NRWKDVLLKLCCSCAIEDNPDDRDAEARVNAVRGLVSVCETLVQGRE SN D++PLLYLI
Sbjct: 901 NRWKDVLLKLCCSCAIEDNPDDRDAEARVNAVRGLVSVCETLVQGREFSNVDELPLLYLI 960
Query: 961 KDEVMASLFKALDDYSVDNRGDVGSWVREAAMNGLEKCTYILCARDSNGFPKKLNGVGSE 1020
KDEVMASLFKALDDYSVDNRGDVGSWVREAAM GLEKCTYILCA DSNGF KKLN VGSE
Sbjct: 961 KDEVMASLFKALDDYSVDNRGDVGSWVREAAMIGLEKCTYILCAIDSNGFTKKLNSVGSE 1020
Query: 1021 PESHHCEMAEKDQIDSFFDSTMATSLVGGICKQAVEKLDKLREAAAMVLQRILYNKIIHV 1080
ES HCEMA+KDQ +SFFDS+MATSLVGGICKQAVEKLDKLREAAAMVLQRILYNK+I V
Sbjct: 1021 QESLHCEMADKDQTNSFFDSSMATSLVGGICKQAVEKLDKLREAAAMVLQRILYNKVILV 1080
Query: 1081 PHIPFQEILEEIVPDDPDMKWGVPAITYPRFVRLLQFGCYSKTVMSGLVISIGGMQDSLS 1140
PHIPF++ LEEI+PDDP+MKW VPA++YP FVRLLQFGCYSKTVMSGLVISIGGMQDSLS
Sbjct: 1081 PHIPFRDTLEEIIPDDPNMKWAVPAVSYPHFVRLLQFGCYSKTVMSGLVISIGGMQDSLS 1140
Query: 1141 KASMSALMEYLEGDAIGDQDKSSRKGILFTDLLWILQRYKKCDRVIIPTFKTIEILFSKR 1200
KAS+SALMEYLEGDAIGDQDK SRKG+LFTDLLWILQRYKKCDRVIIPTFK IEILFSKR
Sbjct: 1141 KASISALMEYLEGDAIGDQDKRSRKGMLFTDLLWILQRYKKCDRVIIPTFKAIEILFSKR 1200
Query: 1201 ILLNVEVHISSFCNGILDSMEIELKGSKDFSKLYAGIAILGYIASLTEPVNSRAFFHLLT 1260
ILLN+EVH+SSFCNGILDS+EIE+KGSKDFSKLYAGI+ILGYI SL+EPVNSRAF HLL+
Sbjct: 1201 ILLNMEVHLSSFCNGILDSLEIEMKGSKDFSKLYAGISILGYITSLSEPVNSRAFVHLLS 1260
Query: 1261 FLSHRYPKIRKVSAEQVYLVLLQNGDFVPENKIEQALEIVSNTCWEGDLENAKLQRRELS 1320
LSHRYPKIRK SAEQVYLVLLQNGDFVPE+KIEQALEIVSNTCWEGDLENAKLQR+ELS
Sbjct: 1261 LLSHRYPKIRKASAEQVYLVLLQNGDFVPEDKIEQALEIVSNTCWEGDLENAKLQRQELS 1266
Query: 1321 DIAGIETDLRPKTNTVPPPEKEVKNRFSAADENASYSSLVESTGF 1366
DIAGIE +L P V PEKEVKNR SA DENA+YSSLVESTGF
Sbjct: 1321 DIAGIEINLHP----VRAPEKEVKNRVSALDENATYSSLVESTGF 1266
BLAST of Spg023281 vs. ExPASy TrEMBL
Match:
A0A0A0K913 (Tubulin-specific chaperone D OS=Cucumis sativus OX=3659 GN=Csa_6G078500 PE=3 SV=1)
HSP 1 Score: 2269.2 bits (5879), Expect = 0.0e+00
Identity = 1166/1366 (85.36%), Postives = 1220/1366 (89.31%), Query Frame = 0
Query: 1 MAATTETAPKKEEVDGLITYDDDDEHESKERVIQKYFLQEWKLVKSILDDIVSNGCVSDI 60
MAATTE APKKEEVDG +YDDDDE+ESKERVIQKYFLQEWKLVKSILDDIVSNG VSDI
Sbjct: 1 MAATTEAAPKKEEVDGTTSYDDDDEYESKERVIQKYFLQEWKLVKSILDDIVSNGRVSDI 60
Query: 61 SAVHKIRSIMQASHSEVVRISSRDAYWAPFMDKYQEQGQLVEPYLDIIVSPLMSLVCSKA 120
S+VHKIRSI MDKYQEQGQLVEPYL+IIVSPLMSLVCSKA
Sbjct: 61 SSVHKIRSI---------------------MDKYQEQGQLVEPYLEIIVSPLMSLVCSKA 120
Query: 121 AELGVASDEVLEVIKPICIIIYTLVTVCGYKAVIRFFPHQVSDLELAVALLERCQNTNSV 180
LGV SD++LEVIKPICIIIYTLVTVCGYKAVIRFFPHQVSDLELAV+LLERCQNTNSV
Sbjct: 121 TALGVESDKLLEVIKPICIIIYTLVTVCGYKAVIRFFPHQVSDLELAVSLLERCQNTNSV 180
Query: 181 TSLRQESTGEMEAQCVILLWLSILVLVPFDISTVDSSLSNASSLAKFEPSPLVLRIVSFC 240
TS RQESTGEMEAQCVILLWLSILVLVPFDIS+VDSSLSN + LA+FEP+PLVLRI+SFC
Sbjct: 181 TSSRQESTGEMEAQCVILLWLSILVLVPFDISSVDSSLSNTNDLAEFEPAPLVLRIISFC 240
Query: 241 KDYLSSAGPMRTMAGLLLARLLTRPDMPKAFISFTEWTHEALSATTNDVMDHFRLLGAAG 300
KDYLSSAGPMRTMAGLLLARLLTRPDMPKAFISFTEWTHEALS TTND+M+HFRLLGAAG
Sbjct: 241 KDYLSSAGPMRTMAGLLLARLLTRPDMPKAFISFTEWTHEALSTTTNDIMNHFRLLGAAG 300
Query: 301 ALASIFKTGDRKLLLDVVPQVWNDTSMLIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHRA 360
ALASIFKTGDRKLLLDVVPQVWNDTSMLIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHRA
Sbjct: 301 ALASIFKTGDRKLLLDVVPQVWNDTSMLIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHRA 360
Query: 361 ASWHYVLLSSSTVDRAKNLVLGVNCVLISKCASLVGCESRTSSLGDNISATVSVGTHKCS 420
ASWHYV SRTSSLGDNISAT+SVGTHKCS
Sbjct: 361 ASWHYV--------------------------------SRTSSLGDNISATMSVGTHKCS 420
Query: 421 PINAVELADVCGSSSSLEDEDMEVPEIIEEIIEMLLTGLKDTDTVVRWSAAKGLGRVTSR 480
PINAVEL++VC S+SLEDEDMEVPEIIEEIIEMLLTGLKDTDTVVRWSAAKGLGRVTSR
Sbjct: 421 PINAVELSNVCQGSTSLEDEDMEVPEIIEEIIEMLLTGLKDTDTVVRWSAAKGLGRVTSR 480
Query: 481 LTSALSEEVLSSVLELFSPGEGDGSWHGGCLALAELARRGLLLPYSLPQVVPIVVKEVEF 540
LTS LSEEVL S+LELFSPGEGDGSWHGGCLALAELARRGLLLP SLPQVVPIVVK
Sbjct: 481 LTSTLSEEVLLSILELFSPGEGDGSWHGGCLALAELARRGLLLPCSLPQVVPIVVK---- 540
Query: 541 VIEVEFDNYSKIVDVFKWVFEEEVVMVKTVVEDVDMEVALHYDIRRGPHSVGSHVRDAAA 600
ALHYDIRRGPHSVGSHVRDAAA
Sbjct: 541 --------------------------------------ALHYDIRRGPHSVGSHVRDAAA 600
Query: 601 YVCWAFGRAYHHTDMREILKQLAPHLLTVACYDREVNCRRAAAAAFQENVGRQGNYPHGI 660
YVCWAFGRAYHHTDMREILKQLAPHLLTVACYDREVNCRRAAAAAFQENVGRQGNYPHGI
Sbjct: 601 YVCWAFGRAYHHTDMREILKQLAPHLLTVACYDREVNCRRAAAAAFQENVGRQGNYPHGI 660
Query: 661 DIVNTADYFSLASRVTSYMKVAVCIAQYEGYLLPFIDELLCNKICHWDKGLRELAADALS 720
DIVN+ADYFSLASRVTSY+KVAVCI QYEGYLLPFIDELLCNKICHWDKGLRELAADALS
Sbjct: 661 DIVNSADYFSLASRVTSYLKVAVCIVQYEGYLLPFIDELLCNKICHWDKGLRELAADALS 720
Query: 721 ALVKYDPEYFASYAVEKLIPCTLSSDLCMRHGATLAVGEVVLSLHQCGHILSSDIQKRVA 780
ALVKYDPEYFASYAVEKLIPCTLSSDLCMRHGATLAVGEVVLSLHQCGHIL SDIQKRVA
Sbjct: 721 ALVKYDPEYFASYAVEKLIPCTLSSDLCMRHGATLAVGEVVLSLHQCGHILPSDIQKRVA 780
Query: 781 GIVPAIEKARLYRGKGGEIMRAAVSRFIECISLSHLPLPEKTKRTLLDALNENLRHPNSQ 840
GIVPAIEKARLYRGKGGEIMRAAVSRFIECISLSHLPL EKTKR LLDALNENLRHPNSQ
Sbjct: 781 GIVPAIEKARLYRGKGGEIMRAAVSRFIECISLSHLPLLEKTKRMLLDALNENLRHPNSQ 840
Query: 841 IQNAAVKSLKPFVPAYLATADAGKSGNVATKYVEQLSDPNVAVRRGSALALSVLPYEFLA 900
IQNAAVKSLKPFVPAYL AD GKSGN+ TKY+EQLSDPNVAVRRGSALALSVLPYE LA
Sbjct: 841 IQNAAVKSLKPFVPAYLVAADTGKSGNIITKYLEQLSDPNVAVRRGSALALSVLPYELLA 900
Query: 901 NRWKDVLLKLCCSCAIEDNPDDRDAEARVNAVRGLVSVCETLVQGRECSNEDDVPLLYLI 960
NRWKDV++KLCC+CAIE+NPDDRDAEARVNAVRGLVSVCETLVQGRECSNED +PLL L+
Sbjct: 901 NRWKDVIMKLCCACAIEENPDDRDAEARVNAVRGLVSVCETLVQGRECSNEDGIPLLCLM 960
Query: 961 KDEVMASLFKALDDYSVDNRGDVGSWVREAAMNGLEKCTYILCARDSNGFPKKLNGVGSE 1020
KDEVM SLFKALDDYSVDNRGDVGSWVREAAMNGLEKCTYILCAR S GF K +N GSE
Sbjct: 961 KDEVMTSLFKALDDYSVDNRGDVGSWVREAAMNGLEKCTYILCARGSCGFTKTVNEFGSE 1020
Query: 1021 PESHHCEMAEKDQ-IDSFFDSTMATSLVGGICKQAVEKLDKLREAAAMVLQRILYNKIIH 1080
PE+ HCE AEKDQ SFFDSTMATSLVGGICKQAVEKLDKLREAAA +LQRILYNKI+H
Sbjct: 1021 PETLHCEKAEKDQTTTSFFDSTMATSLVGGICKQAVEKLDKLREAAATILQRILYNKIVH 1080
Query: 1081 VPHIPFQEILEEIVPDDPDMKWGVPAITYPRFVRLLQFGCYSKTVMSGLVISIGGMQDSL 1140
VPHIPF+EILE+IVPD+ DMKWGVPA++YPRFVRLLQFGCYSKTVMSGLV+S+GGMQDSL
Sbjct: 1081 VPHIPFREILEKIVPDNQDMKWGVPAVSYPRFVRLLQFGCYSKTVMSGLVVSVGGMQDSL 1140
Query: 1141 SKASMSALMEYLEGDAIGDQDKSSRKGILFTDLLWILQRYKKCDRVIIPTFKTIEILFSK 1200
SKASMSALMEYLEGDAIGDQD+SSRKG+LFTD+LWILQRYK+CDRVI+PTFKTIEILFSK
Sbjct: 1141 SKASMSALMEYLEGDAIGDQDESSRKGMLFTDILWILQRYKRCDRVIVPTFKTIEILFSK 1200
Query: 1201 RILLNVEVHISSFCNGILDSMEIELKGSKDFSKLYAGIAILGYIASLTEPVNSRAFFHLL 1260
RI LN+EVHISSFCNGIL S+++ELKGSKDFSKLYAGIAILGYIASL EPVNSRAF +LL
Sbjct: 1201 RI-LNMEVHISSFCNGILGSLDVELKGSKDFSKLYAGIAILGYIASLPEPVNSRAFSYLL 1260
Query: 1261 TFLSHRYPKIRKVSAEQVYLVLLQNGDFVPENKIEQALEIVSNTCWEGDLENAKLQRREL 1320
TFLSHRYPKIRK SAEQVYLVLLQNG+FVPENKI++ALEIVSNTCWEGDLENAKLQRREL
Sbjct: 1261 TFLSHRYPKIRKASAEQVYLVLLQNGNFVPENKIDEALEIVSNTCWEGDLENAKLQRREL 1270
Query: 1321 SDIAGIETDLRPKTNTVPPPEKEVKNRFSAADENASYSSLVESTGF 1366
DIAGIETD+ KTN VPPPEKEVKNRFS ADENASYSSLVESTGF
Sbjct: 1321 YDIAGIETDIHSKTNMVPPPEKEVKNRFSGADENASYSSLVESTGF 1270
BLAST of Spg023281 vs. ExPASy TrEMBL
Match:
A0A5A7TFF8 (Tubulin-specific chaperone D OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00550 PE=3 SV=1)
HSP 1 Score: 2248.0 bits (5824), Expect = 0.0e+00
Identity = 1157/1358 (85.20%), Postives = 1206/1358 (88.81%), Query Frame = 0
Query: 8 APKKEEVDGLITYDDDDEHESKERVIQKYFLQEWKLVKSILDDIVSNGCVSDISAVHKIR 67
A K EEVDG+ TYDDDDEHESKERVIQKYFLQEWKLVKSILDDIVSNG VSDIS+VHKIR
Sbjct: 2 AAKNEEVDGMTTYDDDDEHESKERVIQKYFLQEWKLVKSILDDIVSNGRVSDISSVHKIR 61
Query: 68 SIMQASHSEVVRISSRDAYWAPFMDKYQEQGQLVEPYLDIIVSPLMSLVCSKAAELGVAS 127
SI MDKYQEQGQLVEPYL+IIVSPLMSLVCSKA LGV S
Sbjct: 62 SI---------------------MDKYQEQGQLVEPYLEIIVSPLMSLVCSKATALGVDS 121
Query: 128 DEVLEVIKPICIIIYTLVTVCGYKAVIRFFPHQVSDLELAVALLERCQNTNSVTSLRQES 187
+++LEVIKPICIIIYTLVTVCGYKAVIRFFPHQVSDLELAV+LLERCQNTNSVTS RQES
Sbjct: 122 EKLLEVIKPICIIIYTLVTVCGYKAVIRFFPHQVSDLELAVSLLERCQNTNSVTSSRQES 181
Query: 188 TGEMEAQCVILLWLSILVLVPFDISTVDSSLSNASSLAKFEPSPLVLRIVSFCKDYLSSA 247
TGEMEAQCVILLWLSILVLVPFDIS+VDSSLSN ++LA+FEP+PLVLRI+SFCKDYLSSA
Sbjct: 182 TGEMEAQCVILLWLSILVLVPFDISSVDSSLSNTNNLAEFEPAPLVLRIISFCKDYLSSA 241
Query: 248 GPMRTMAGLLLARLLTRPDMPKAFISFTEWTHEALSATTNDVMDHFRLLGAAGALASIFK 307
GPMR MAGLL+ARLLTRPDMPKAFISF WTHEALS TTND+M+ FRLLGA GALASIFK
Sbjct: 242 GPMRAMAGLLVARLLTRPDMPKAFISFIGWTHEALSTTTNDIMNQFRLLGATGALASIFK 301
Query: 308 TGDRKLLLDVVPQVWNDTSMLIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHRAASWHYVL 367
TGDRKLLLDVVPQVWNDTSMLIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHRAASWHYV
Sbjct: 302 TGDRKLLLDVVPQVWNDTSMLIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHRAASWHYV- 361
Query: 368 LSSSTVDRAKNLVLGVNCVLISKCASLVGCESRTSSLGDNISATVSVGTHKCSPINAVEL 427
SRTSSLGDNISAT+SVGTH CS IN VEL
Sbjct: 362 -------------------------------SRTSSLGDNISATMSVGTHNCSSINTVEL 421
Query: 428 ADVCGSSSSLEDEDMEVPEIIEEIIEMLLTGLKDTDTVVRWSAAKGLGRVTSRLTSALSE 487
++VC S+SLEDEDMEVPEIIEEIIEMLLTGLKDTDTVVRWSAAKGLGRVTSRLTS LSE
Sbjct: 422 SNVCQGSTSLEDEDMEVPEIIEEIIEMLLTGLKDTDTVVRWSAAKGLGRVTSRLTSTLSE 481
Query: 488 EVLSSVLELFSPGEGDGSWHGGCLALAELARRGLLLPYSLPQVVPIVVKEVEFVIEVEFD 547
EVLSS+LELFSPGEGDGSWHGGCLALAELARRGLLLP SLPQVVPIVVK
Sbjct: 482 EVLSSILELFSPGEGDGSWHGGCLALAELARRGLLLPCSLPQVVPIVVK----------- 541
Query: 548 NYSKIVDVFKWVFEEEVVMVKTVVEDVDMEVALHYDIRRGPHSVGSHVRDAAAYVCWAFG 607
ALHYDIRRGPHSVGSHVRDAAAYVCWAFG
Sbjct: 542 -------------------------------ALHYDIRRGPHSVGSHVRDAAAYVCWAFG 601
Query: 608 RAYHHTDMREILKQLAPHLLTVACYDREVNCRRAAAAAFQENVGRQGNYPHGIDIVNTAD 667
RAYHHTDMREILKQLAPHLLTVACYDREVNCRRAAAAAFQENVGRQGNYPHGIDIVN+AD
Sbjct: 602 RAYHHTDMREILKQLAPHLLTVACYDREVNCRRAAAAAFQENVGRQGNYPHGIDIVNSAD 661
Query: 668 YFSLASRVTSYMKVAVCIAQYEGYLLPFIDELLCNKICHWDKGLRELAADALSALVKYDP 727
YFSLASRVTSY+KVAVCIAQYEGYLLPFIDELLCNKICHWDKGLRELAADALSALVKYDP
Sbjct: 662 YFSLASRVTSYLKVAVCIAQYEGYLLPFIDELLCNKICHWDKGLRELAADALSALVKYDP 721
Query: 728 EYFASYAVEKLIPCTLSSDLCMRHGATLAVGEVVLSLHQCGHILSSDIQKRVAGIVPAIE 787
EYFASYAVEKLIPCTLSSDLCMRHGATLAVGEVVLSLHQCGHIL SDIQKRVAGIVPAIE
Sbjct: 722 EYFASYAVEKLIPCTLSSDLCMRHGATLAVGEVVLSLHQCGHILPSDIQKRVAGIVPAIE 781
Query: 788 KARLYRGKGGEIMRAAVSRFIECISLSHLPLPEKTKRTLLDALNENLRHPNSQIQNAAVK 847
KARLYRGKGGEIMRAAVSRFIECISLSHLPL EKTKR LLDALNENLRHPNSQIQNAAVK
Sbjct: 782 KARLYRGKGGEIMRAAVSRFIECISLSHLPLLEKTKRMLLDALNENLRHPNSQIQNAAVK 841
Query: 848 SLKPFVPAYLATADAGKSGNVATKYVEQLSDPNVAVRRGSALALSVLPYEFLANRWKDVL 907
SLKPFVPAYL AD GKSGN+ TKY+EQL DPNVAVRRGSALALSVLPYE LANRWKDV+
Sbjct: 842 SLKPFVPAYLVAADTGKSGNITTKYLEQLKDPNVAVRRGSALALSVLPYELLANRWKDVV 901
Query: 908 LKLCCSCAIEDNPDDRDAEARVNAVRGLVSVCETLVQGRECSNEDDVPLLYLIKDEVMAS 967
+KLCC+CAIE+NPDDRDAEARVNAVRGLVSVCETLVQGRECSNED +PLL LIKDEVM S
Sbjct: 902 MKLCCACAIEENPDDRDAEARVNAVRGLVSVCETLVQGRECSNEDGIPLLCLIKDEVMTS 961
Query: 968 LFKALDDYSVDNRGDVGSWVREAAMNGLEKCTYILCARDSNGFPKKLNGVGSEPESHHCE 1027
LFKALDDYSVDNRGDVGSWVREAAMNGLEKCTYILCAR S F K LNGVGSEPE+ HCE
Sbjct: 962 LFKALDDYSVDNRGDVGSWVREAAMNGLEKCTYILCARGSCVFTKTLNGVGSEPETLHCE 1021
Query: 1028 MAEKDQIDSFFDSTMATSLVGGICKQAVEKLDKLREAAAMVLQRILYNKIIHVPHIPFQE 1087
AEKDQ SFFDSTMATSLVGGICKQAVEKLDKLREAAA +LQRILYNKIIHVPHIPF+E
Sbjct: 1022 KAEKDQTTSFFDSTMATSLVGGICKQAVEKLDKLREAAATILQRILYNKIIHVPHIPFRE 1081
Query: 1088 ILEEIVPDDPDMKWGVPAITYPRFVRLLQFGCYSKTVMSGLVISIGGMQDSLSKASMSAL 1147
ILE+IVPDD DMKWGVPA++YPRFVRLLQFGCYSKTVMSGLVISIGGMQDSLSKASMSAL
Sbjct: 1082 ILEKIVPDDQDMKWGVPAVSYPRFVRLLQFGCYSKTVMSGLVISIGGMQDSLSKASMSAL 1141
Query: 1148 MEYLEGDAIGDQDKSSRKGILFTDLLWILQRYKKCDRVIIPTFKTIEILFSKRILLNVEV 1207
MEYLEGDAIGDQD+SSRKG+LFTDLLWILQRYK+CDRVI+PTFKTIEILFSKRI LN+EV
Sbjct: 1142 MEYLEGDAIGDQDESSRKGMLFTDLLWILQRYKRCDRVIVPTFKTIEILFSKRI-LNMEV 1201
Query: 1208 HISSFCNGILDSMEIELKGSKDFSKLYAGIAILGYIASLTEPVNSRAFFHLLTFLSHRYP 1267
HISSFCNG L S+++ELKGSKDFSKLYAGIAILGYIASL EPVNSRAF +LLT LSHRYP
Sbjct: 1202 HISSFCNGTLGSLDVELKGSKDFSKLYAGIAILGYIASLPEPVNSRAFSYLLTLLSHRYP 1261
Query: 1268 KIRKVSAEQVYLVLLQNGDFVPENKIEQALEIVSNTCWEGDLENAKLQRRELSDIAGIET 1327
KIRK SAEQVYLVLLQNG+FVPENKIE+ALEIVSNTCWEGDLENAKLQRRELSDIAGIET
Sbjct: 1262 KIRKASAEQVYLVLLQNGNFVPENKIEEALEIVSNTCWEGDLENAKLQRRELSDIAGIET 1263
Query: 1328 DLRPKTNTVPPPEKEVKNRFSAADENASYSSLVESTGF 1366
D+ P+TN VP EKEVKNRFS ADENASYSSLVESTGF
Sbjct: 1322 DIHPRTNLVPSTEKEVKNRFSGADENASYSSLVESTGF 1263
BLAST of Spg023281 vs. ExPASy TrEMBL
Match:
A0A6J1DR52 (Tubulin-specific chaperone D OS=Momordica charantia OX=3673 GN=LOC111022784 PE=3 SV=1)
HSP 1 Score: 2247.2 bits (5822), Expect = 0.0e+00
Identity = 1161/1365 (85.05%), Postives = 1210/1365 (88.64%), Query Frame = 0
Query: 1 MAATTETAPKKEEVDGLITYDDDDEHESKERVIQKYFLQEWKLVKSILDDIVSNGCVSDI 60
MAAT AP K E DG TYDDDDE ESKERVI KYFLQEWKLV SIL DI+SNGCVSDI
Sbjct: 1 MAATIGAAPTK-EFDGTKTYDDDDERESKERVIHKYFLQEWKLVDSILHDILSNGCVSDI 60
Query: 61 SAVHKIRSIMQASHSEVVRISSRDAYWAPFMDKYQEQGQLVEPYLDIIVSPLMSLVCSKA 120
S+VHKIRSI MDKYQEQGQLVEPYL+ IVSPLMSLVCSK
Sbjct: 61 SSVHKIRSI---------------------MDKYQEQGQLVEPYLESIVSPLMSLVCSKT 120
Query: 121 AELGVASDEVLEVIKPICIIIYTLVTVCGYKAVIRFFPHQVSDLELAVALLERCQNTNSV 180
ELGVASD ++EVIKPICI+IYTLVTVCGYKAVIRFFPHQVSDLELAV+LLERCQNTNSV
Sbjct: 121 MELGVASDRLIEVIKPICIVIYTLVTVCGYKAVIRFFPHQVSDLELAVSLLERCQNTNSV 180
Query: 181 TSLRQESTGEMEAQCVILLWLSILVLVPFDISTVDSSLSNASSLAKFEPSPLVLRIVSFC 240
T+LRQESTGEMEAQCVILLWLSILVLVPFDISTVDSSLSN +SL KFEPSPLVLRI+SFC
Sbjct: 181 TTLRQESTGEMEAQCVILLWLSILVLVPFDISTVDSSLSNTNSLGKFEPSPLVLRIISFC 240
Query: 241 KDYLSSAGPMRTMAGLLLARLLTRPDMPKAFISFTEWTHEALSATTNDVMDHFRLLGAAG 300
KDYLSSAGPMRTMAGLLLARLLTRPDMPKAFISF EWTHEALSATTND+MDHFRLLGAAG
Sbjct: 241 KDYLSSAGPMRTMAGLLLARLLTRPDMPKAFISFIEWTHEALSATTNDIMDHFRLLGAAG 300
Query: 301 ALASIFKTGDRKLLLDVVPQVWNDTSMLIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHRA 360
ALASIFK GDRKLL+DVVPQVWNDTS LIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHRA
Sbjct: 301 ALASIFKVGDRKLLIDVVPQVWNDTSTLIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHRA 360
Query: 361 ASWHYVLLSSSTVDRAKNLVLGVNCVLISKCASLVGCESRTSSLGDNISATVSVGTHKCS 420
ASWHYV SRTSSLGD+ISATVSVGTHKCS
Sbjct: 361 ASWHYV--------------------------------SRTSSLGDSISATVSVGTHKCS 420
Query: 421 PINAVELADVCGSSSSLEDEDMEVPEIIEEIIEMLLTGLKDTDTVVRWSAAKGLGRVTSR 480
P++AVEL+ VC +S+SLEDEDM+VPEIIEEIIEMLL+GLKDTDTVVRWSAAKGLGRVTSR
Sbjct: 421 PVHAVELSHVCQTSTSLEDEDMDVPEIIEEIIEMLLSGLKDTDTVVRWSAAKGLGRVTSR 480
Query: 481 LTSALSEEVLSSVLELFSPGEGDGSWHGGCLALAELARRGLLLPYSLPQVVPIVVKEVEF 540
LTSALSEEVLSSVLELFSPGEGDGSWHGGCLALAELARRGLLLPYSLPQVVPIVVK
Sbjct: 481 LTSALSEEVLSSVLELFSPGEGDGSWHGGCLALAELARRGLLLPYSLPQVVPIVVK---- 540
Query: 541 VIEVEFDNYSKIVDVFKWVFEEEVVMVKTVVEDVDMEVALHYDIRRGPHSVGSHVRDAAA 600
ALHYD+RRGPHSVGSHVRDAAA
Sbjct: 541 --------------------------------------ALHYDVRRGPHSVGSHVRDAAA 600
Query: 601 YVCWAFGRAYHHTDMREILKQLAPHLLTVACYDREVNCRRAAAAAFQENVGRQGNYPHGI 660
YVCWAFGRAYHHTDMREILKQLAPHLLTVACYDREVNCRRAAAAAFQENVGRQG+YPHGI
Sbjct: 601 YVCWAFGRAYHHTDMREILKQLAPHLLTVACYDREVNCRRAAAAAFQENVGRQGSYPHGI 660
Query: 661 DIVNTADYFSLASRVTSYMKVAVCIAQYEGYLLPFIDELLCNKICHWDKGLRELAADALS 720
DIVNTADYFSLASRVTSY+KVAV IAQYEGYLLPFIDELLCNKICHWDKGLRELAADALS
Sbjct: 661 DIVNTADYFSLASRVTSYLKVAVSIAQYEGYLLPFIDELLCNKICHWDKGLRELAADALS 720
Query: 721 ALVKYDPEYFASYAVEKLIPCTLSSDLCMRHGATLAVGEVVLSLHQCGHILSSDIQKRVA 780
ALVKYDPE FASY VEKLIPCTLSSDLC RHGATLAVGEVVLSLHQCGHILSSDIQKRVA
Sbjct: 721 ALVKYDPECFASYVVEKLIPCTLSSDLCTRHGATLAVGEVVLSLHQCGHILSSDIQKRVA 780
Query: 781 GIVPAIEKARLYRGKGGEIMRAAVSRFIECISLSHLPLPEKTKRTLLDALNENLRHPNSQ 840
IVPAIEKARLYRGKGGEIMRAAVSRFIECIS SHLPL EKTKR LLD LNENLRHPNSQ
Sbjct: 781 SIVPAIEKARLYRGKGGEIMRAAVSRFIECISSSHLPLLEKTKRALLDTLNENLRHPNSQ 840
Query: 841 IQNAAVKSLKPFVPAYLATADAGKSGNVATKYVEQLSDPNVAVRRGSALALSVLPYEFLA 900
IQNAAVKSLKPFVPAYLA ADAGKSGN+ KY+EQLSDPNVAVRRGSALALS+LPYEFLA
Sbjct: 841 IQNAAVKSLKPFVPAYLAAADAGKSGNITAKYLEQLSDPNVAVRRGSALALSILPYEFLA 900
Query: 901 NRWKDVLLKLCCSCAIEDNPDDRDAEARVNAVRGLVSVCETLVQGRECSNEDDVPLLYLI 960
NRWKDVLLKLCCSCAIEDNPDDRDAEARVNAVRGLVSVCETLVQGRECSNEDD+PLL LI
Sbjct: 901 NRWKDVLLKLCCSCAIEDNPDDRDAEARVNAVRGLVSVCETLVQGRECSNEDDIPLLQLI 960
Query: 961 KDEVMASLFKALDDYSVDNRGDVGSWVREAAMNGLEKCTYILCARDSNGFPKKLNGVGSE 1020
KDEVMASLFKALDDYSVDNRGDVGSWVREAAMNGLE+CTYILCARDSNGF K+LNGVGSE
Sbjct: 961 KDEVMASLFKALDDYSVDNRGDVGSWVREAAMNGLERCTYILCARDSNGFTKRLNGVGSE 1020
Query: 1021 PESHHCEMAEKDQIDSFFDSTMATSLVGGICKQAVEKLDKLREAAAMVLQRILYNKIIHV 1080
PES H EMAEKDQ +SFFDSTMATSLVGGICKQAVEKLDKLREAAA VL++ILYNK IHV
Sbjct: 1021 PESPHWEMAEKDQTNSFFDSTMATSLVGGICKQAVEKLDKLREAAATVLRQILYNKSIHV 1080
Query: 1081 PHIPFQEILEEIVPDDPDMKWGVPAITYPRFVRLLQFGCYSKTVMSGLVISIGGMQDSLS 1140
PHIPF+EILEEIVP+DPDMKWGVPA+TYPRFV+LL+FGCYSKTVMSGLVISIGGMQDSLS
Sbjct: 1081 PHIPFREILEEIVPEDPDMKWGVPAVTYPRFVQLLRFGCYSKTVMSGLVISIGGMQDSLS 1140
Query: 1141 KASMSALMEYLEGDAIGDQDKSSRKGILFTDLLWILQRYKKCDRVIIPTFKTIEILFSKR 1200
K SMSALMEYLEGDAIGDQDKSSRK +LFTDLLWILQ YK+ DRVIIPTFKTIEILFS+R
Sbjct: 1141 KVSMSALMEYLEGDAIGDQDKSSRKAMLFTDLLWILQSYKRRDRVIIPTFKTIEILFSRR 1200
Query: 1201 ILLNVEVHISSFCNGILDSMEIELKGSKDFSKLYAGIAILGYIASLTEPVNSRAFFHLLT 1260
ILLN+EVH SSFC G+LDS+EIELKGSKDFSKLYAGIAILGYIASL+E VN+RAF LL
Sbjct: 1201 ILLNIEVHTSSFCYGVLDSLEIELKGSKDFSKLYAGIAILGYIASLSESVNARAFCQLLA 1260
Query: 1261 FLSHRYPKIRKVSAEQVYLVLLQNGDFVPENKIEQALEIVSNTCWEGDLENAKLQRRELS 1320
FLSHRYPKIRK SAEQVYLVLLQNGD VPENKIEQALEIVSNTCWEGD+ENAKLQR+ELS
Sbjct: 1261 FLSHRYPKIRKASAEQVYLVLLQNGDLVPENKIEQALEIVSNTCWEGDIENAKLQRQELS 1268
Query: 1321 DIAGIETDLRPKTNTVPPPEKEVKNRFSAADENASYSSLVESTGF 1366
DIAGIETDL PKT+TV PEKEVKN +A DENASYSSLVESTGF
Sbjct: 1321 DIAGIETDLHPKTHTV-QPEKEVKNLSTATDENASYSSLVESTGF 1268
BLAST of Spg023281 vs. TAIR 10
Match:
AT3G60740.1 (ARM repeat superfamily protein )
HSP 1 Score: 1604.0 bits (4152), Expect = 0.0e+00
Identity = 847/1364 (62.10%), Postives = 1023/1364 (75.00%), Query Frame = 0
Query: 6 ETAPKKEEVDGL--ITYDDDDEHESKERVIQKYFLQEWKLVKSILDDIVSNGCVSDISAV 65
E +P K E++ I ++DDEH+SKERV+Q+YFLQEWKLVKS+LDDIVSNG V D ++V
Sbjct: 7 EMSPTKIEIETAVKIEEEEDDEHDSKERVLQRYFLQEWKLVKSLLDDIVSNGRVVDPTSV 66
Query: 66 HKIRSIMQASHSEVVRISSRDAYWAPFMDKYQEQGQLVEPYLDIIVSPLMSLVCSKAAEL 125
HKIRSI MDKYQEQGQLVEPYL+ IVSPLM ++ SK +L
Sbjct: 67 HKIRSI---------------------MDKYQEQGQLVEPYLESIVSPLMFIIRSKTVDL 126
Query: 126 GVASDEVLEVIKPICIIIYTLVTVCGYKAVIRFFPHQVSDLELAVALLERCQNTNSVTSL 185
DE+LE+IKPI IIIY LVTVCGYKAVI+FFPHQVSDLELAV LLE+C +TNSV++L
Sbjct: 127 EAKPDEILEIIKPISIIIYALVTVCGYKAVIKFFPHQVSDLELAVLLLEKCHSTNSVSAL 186
Query: 186 RQESTGEMEAQCVILLWLSILVLVPFDISTVDSSLSNASSLAKFEPSPLVLRIVSFCKDY 245
RQESTGEMEA+CV LLWLSILVLVPFDIS+VD+S+++ + + +PLVL+I+ FCKDY
Sbjct: 187 RQESTGEMEAKCVTLLWLSILVLVPFDISSVDTSIADDKTFGVDDLAPLVLKILGFCKDY 246
Query: 246 LSSAGPMRTMAGLLLARLLTRPDMPKAFISFTEWTHEALSATTNDVMDHFRLLGAAGALA 305
L SAGPMR ++GLLL++LLTRPDM KAF SF EWT+E LS + V +HFRLLG AL+
Sbjct: 247 LCSAGPMRRISGLLLSKLLTRPDMGKAFSSFFEWTYEVLSCKEDSVTNHFRLLGVMEALS 306
Query: 306 SIFKTGDRKLLLDVVPQVWNDTSMLIKSNTAVRSPLLRKYLLKLTQRIGLTCLPHRAASW 365
+IFKT RK+LLDV+P V ND ++L KSN A +S LLRKYL+KLTQRIGL CLPHR+ SW
Sbjct: 307 AIFKTASRKVLLDVLPIVLNDVTVLSKSNAAAKSSLLRKYLIKLTQRIGLVCLPHRSPSW 366
Query: 366 HYVLLSSSTVDRAKNLVLGVNCVLISKCASLVGCESRTSSLGDNISATVSVGTHKCSPIN 425
YV ++T+SL +N+S + S + +P +
Sbjct: 367 RYV--------------------------------AQTASLSENMSTSSS---QRLAPDH 426
Query: 426 AVELADVCGSSSSLEDEDMEVPEIIEEIIEMLLTGLKDTDTVVRWSAAKGLGRVTSRLTS 485
V S EDEDM+VPEI+EEIIEMLL+GL+DTDTVVRWSAAKG+GRVTSRLTS
Sbjct: 427 TVTAILQPESLDDQEDEDMDVPEILEEIIEMLLSGLRDTDTVVRWSAAKGIGRVTSRLTS 486
Query: 486 ALSEEVLSSVLELFSPGEGDGSWHGGCLALAELARRGLLLPYSLPQVVPIVVKEVEFVIE 545
LS+EVLSSVLELFSPGEGDGSWHGGCLALAELARRGLLLP S P VVP++VK
Sbjct: 487 VLSDEVLSSVLELFSPGEGDGSWHGGCLALAELARRGLLLPRSFPLVVPVIVK------- 546
Query: 546 VEFDNYSKIVDVFKWVFEEEVVMVKTVVEDVDMEVALHYDIRRGPHSVGSHVRDAAAYVC 605
ALHYD+RRGPHSVGSHVRDAAAYVC
Sbjct: 547 -----------------------------------ALHYDVRRGPHSVGSHVRDAAAYVC 606
Query: 606 WAFGRAYHHTDMREILKQLAPHLLTVACYDREVNCRRAAAAAFQENVGRQGNYPHGIDIV 665
WAFGRAY H DM+ +L QLAP LL V +DREVNCRRAAAAAFQENVGRQGNYPHGIDIV
Sbjct: 607 WAFGRAYSHKDMKNVLDQLAPDLLIVGSFDREVNCRRAAAAAFQENVGRQGNYPHGIDIV 666
Query: 666 NTADYFSLASRVTSYMKVAVCIAQYEGYLLPFIDELLCNKICHWDKGLRELAADALSALV 725
+ ADYFSL+SRV SY++VAV IAQYEGYL PF+DELL NKICHWDK LRELAA+AL+ LV
Sbjct: 667 SIADYFSLSSRVNSYLQVAVSIAQYEGYLYPFVDELLYNKICHWDKSLRELAAEALAPLV 726
Query: 726 KYDPEYFASYAVEKLIPCTLSSDLCMRHGATLAVGEVVLSLHQCGHILSSDIQKRVAGIV 785
KY+P++FA+Y +EKLIPCTLS+DLCMRHGATLA GEVVL+LHQCG++LS+D QKR+AGIV
Sbjct: 727 KYEPKHFANYVLEKLIPCTLSTDLCMRHGATLAAGEVVLALHQCGYVLSADSQKRMAGIV 786
Query: 786 PAIEKARLYRGKGGEIMRAAVSRFIECISLSHLPLPEKTKRTLLDALNENLRHPNSQIQN 845
P+IEKARLYRGKGGEIMR AVSRFIECISLSH+ L E+T+R LLD L ENLRHPNSQIQN
Sbjct: 787 PSIEKARLYRGKGGEIMRLAVSRFIECISLSHVTLAERTERILLDTLTENLRHPNSQIQN 846
Query: 846 AAVKSLKPFVPAYLATADAGKSGNVATKYVEQLSDPNVAVRRGSALALSVLPYEFLANRW 905
AAV ++K V +YL D KS ++ K+++ L+DPNVAVRRGSALAL VLPYE L +W
Sbjct: 847 AAVSAVKQLVQSYLVGNDK-KSVDLILKHLKHLTDPNVAVRRGSALALGVLPYELLTAKW 906
Query: 906 KDVLLKLCCSCAIEDNPDDRDAEARVNAVRGLVSVCETLVQGRECS-NEDDVPLLYLIKD 965
KD++LKLC +C IE NP+DRDAEARVNAV+GL SVCETL Q R DD+ L LIK
Sbjct: 907 KDIVLKLCSACKIEVNPEDRDAEARVNAVKGLTSVCETLTQKRASDPGNDDLSLFLLIKT 966
Query: 966 EVMASLFKALDDYSVDNRGDVGSWVREAAMNGLEKCTYILCARDSNGFPKKLNGVGSEPE 1025
EVM +L KALDDYSVDNRGDVGSWVREAA++GLEKCTYILC KK+
Sbjct: 967 EVMDTLLKALDDYSVDNRGDVGSWVREAAVHGLEKCTYILC--------KKM-------- 1026
Query: 1026 SHHCEMAEKDQIDSFFDSTMATSLVGGICKQAVEKLDKLREAAAMVLQRILYNKIIHVPH 1085
+ E D S FDS +AT L+GG+ KQ VEK+DKLRE AA VLQRILY+K + VPH
Sbjct: 1027 ETYSEGDYNDDTSSLFDSNLATRLIGGMLKQGVEKMDKLRETAAKVLQRILYHKSVSVPH 1086
Query: 1086 IPFQEILEEIVPDDPDMKWGVPAITYPRFVRLLQFGCYSKTVMSGLVISIGGMQDSLSKA 1145
+P++E LEEI+P+ +++W VPA ++PRFV+LL+ CYSK VMSGLVISIGG+QDSL KA
Sbjct: 1087 VPYREKLEEILPNKANLQWAVPAFSFPRFVQLLKLRCYSKEVMSGLVISIGGLQDSLRKA 1146
Query: 1146 SMSALMEYL-EGDAIGDQDKSSRKGILFTDLLWILQRYKKCDRVIIPTFKTIEILFSKRI 1205
S+ AL+EY+ EG+A +++ SR+ L D+LWILQ YKKCDRV++P +TIEILFS +I
Sbjct: 1147 SLVALLEYMREGEAKDPKEQQSRESALGDDILWILQEYKKCDRVMVPCLQTIEILFSSKI 1206
Query: 1206 LLNVEVHISSFCNGILDSMEIELKGSKDFSKLYAGIAILGYIASLTEPVNSRAFFHLLTF 1265
LN E + SF G++DS+ IEL+ SKDF+KL AG+AILGYIAS++ ++++AF LL+F
Sbjct: 1207 FLNQESYTFSFYAGVMDSLAIELRASKDFTKLKAGLAILGYIASVSHFISTKAFSQLLSF 1254
Query: 1266 LSHRYPKIRKVSAEQVYLVLLQNGDFVPENKIEQALEIVSNTCWEGDLENAKLQRRELSD 1325
L HRYP IRK +AEQVYL LLQNG V E K+E+ +EI+S +CWE D+E K QR EL +
Sbjct: 1267 LGHRYPMIRKAAAEQVYLALLQNGILVTEEKMEKVIEIISESCWEADMETTKTQRLELCE 1254
Query: 1326 IAGIETDLRPKTNTVPPPEKEVKNRFSAADENASYSSLVESTGF 1366
+AG++ ++ KT K++ SAADENASYSSLV+S+GF
Sbjct: 1327 LAGLDHEVVFKTRN-RLVIKDIAGNKSAADENASYSSLVDSSGF 1254
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_023514261.1 | 0.0e+00 | 86.30 | tubulin-folding cofactor D isoform X1 [Cucurbita pepo subsp. pepo] | [more] |
| XP_023000016.1 | 0.0e+00 | 86.01 | tubulin-folding cofactor D isoform X1 [Cucurbita maxima] | [more] |
| XP_038906763.1 | 0.0e+00 | 85.80 | tubulin-folding cofactor D [Benincasa hispida] | [more] |
| XP_022964337.1 | 0.0e+00 | 86.01 | tubulin-folding cofactor D isoform X1 [Cucurbita moschata] | [more] |
| XP_004145065.1 | 0.0e+00 | 85.36 | tubulin-folding cofactor D [Cucumis sativus] >KGN46255.1 hypothetical protein Cs... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q8L5R3 | 0.0e+00 | 62.10 | Tubulin-folding cofactor D OS=Arabidopsis thaliana OX=3702 GN=TFCD PE=2 SV=1 | [more] |
| Q9BTW9 | 7.3e-171 | 33.28 | Tubulin-specific chaperone D OS=Homo sapiens OX=9606 GN=TBCD PE=1 SV=2 | [more] |
| Q28205 | 4.1e-166 | 32.94 | Tubulin-specific chaperone D OS=Bos taurus OX=9913 GN=TBCD PE=1 SV=1 | [more] |
| Q8BYA0 | 2.5e-163 | 32.21 | Tubulin-specific chaperone D OS=Mus musculus OX=10090 GN=Tbcd PE=1 SV=1 | [more] |
| Q5ZI87 | 8.4e-143 | 32.87 | Tubulin-specific chaperone D OS=Gallus gallus OX=9031 GN=TBCD PE=2 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1KEP9 | 0.0e+00 | 86.01 | Tubulin-specific chaperone D OS=Cucurbita maxima OX=3661 GN=LOC111494326 PE=3 SV... | [more] |
| A0A6J1HHJ4 | 0.0e+00 | 86.01 | Tubulin-specific chaperone D OS=Cucurbita moschata OX=3662 GN=LOC111464377 PE=3 ... | [more] |
| A0A0A0K913 | 0.0e+00 | 85.36 | Tubulin-specific chaperone D OS=Cucumis sativus OX=3659 GN=Csa_6G078500 PE=3 SV=... | [more] |
| A0A5A7TFF8 | 0.0e+00 | 85.20 | Tubulin-specific chaperone D OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... | [more] |
| A0A6J1DR52 | 0.0e+00 | 85.05 | Tubulin-specific chaperone D OS=Momordica charantia OX=3673 GN=LOC111022784 PE=3... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT3G60740.1 | 0.0e+00 | 62.10 | ARM repeat superfamily protein | [more] |
