Spg023271 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg023271 vs. NCBI nr
Match: XP_038906949.1 (uncharacterized protein LOC120092818 [Benincasa hispida])

HSP 1 Score: 905.6 bits (2339), Expect = 2.0e-259
Identity = 481/540 (89.07%), Postives = 500/540 (92.59%), Query Frame = 0

Query: 1   MRTYAVAWVHPDRKLSTRVDTHGHNNPTWNDKFVFRVDDEFLHADTSAVMIEIYALHWFK 60
           MRTYAVAWVHPDRKLSTRVDTHGHNNPTWNDKFVFRVDDEFLH+DTSAVMIEIYALHWFK
Sbjct: 27  MRTYAVAWVHPDRKLSTRVDTHGHNNPTWNDKFVFRVDDEFLHSDTSAVMIEIYALHWFK 86

Query: 61  DIHVGTVRILVGNLIPTPPRLHQFSQQPQVGMRFVALQVRRPSGRPQGILNIGVALLHSS 120
           DIHVGTVRILVGNLIPTPPRLHQFSQQPQVGMRFVALQVRRPSGRPQGILNIGVALLHSS
Sbjct: 87  DIHVGTVRILVGNLIPTPPRLHQFSQQPQVGMRFVALQVRRPSGRPQGILNIGVALLHSS 146

Query: 121 MRSMPLYSQLSTSAIGYRNLMGEEDPFHNHHSNTNNHSHGTPTVVTRPEMRRSKSDTSSM 180
           MRSMPLYSQLSTSAIGYRNLMGEEDPF NHHSN NNHSHGTPTVVTRPEMRRSKSDTSSM
Sbjct: 147 MRSMPLYSQLSTSAIGYRNLMGEEDPFANHHSNNNNHSHGTPTVVTRPEMRRSKSDTSSM 206

Query: 181 MGSELRGSEMMGKGMKSKASSMINGSEVSKHKKKGRSKASSMISGSVVIKRKGKSRKASS 240
           MGSELRGSEMMG+G KSKASSM+NGSEVSK KKKGRSKASSMISGSVVIK+KGKSRKASS
Sbjct: 207 MGSELRGSEMMGRGTKSKASSMVNGSEVSKQKKKGRSKASSMISGSVVIKKKGKSRKASS 266

Query: 241 MLSGSGIEEAYNKQNGKNGKSNAVIVGKKEKTEPSSILSGSEVYPIELPPNKKETPVHKP 300
           M+SGSGIEEAYN  NGKNGKSN VI GKK+K +  SILSGSEV+PIE P NKKE    KP
Sbjct: 267 MISGSGIEEAYNVTNGKNGKSNLVIAGKKDKIDQYSILSGSEVHPIEPPTNKKEKAAQKP 326

Query: 301 NSSEETFDSPPWKKP-QEKPISRPEVTDSPRGKPGQETPNSRFDKMPYATPLRTPQRL-A 360
           NSSE++ DSPPWKKP  +KP+S     DSPRGK GQETPNSRFDKMPYATPLRTPQRL A
Sbjct: 327 NSSEKSLDSPPWKKPSNDKPVS-----DSPRGKQGQETPNSRFDKMPYATPLRTPQRLAA 386

Query: 361 GGGGTARATPMHEKFATPRRTPTPLHGKSSHTKYNNDFNYTTPRKSFLGGAILTESELGP 420
            GGGTARATPMH+KFATPRR+ TPL GKSS+TKY N+FNYTTPRKSFLGGA LTESELGP
Sbjct: 387 SGGGTARATPMHDKFATPRRS-TPL-GKSSYTKY-NEFNYTTPRKSFLGGAFLTESELGP 446

Query: 421 SPSEVAAQMATQKVDD-NESSIVGGLSLNDSVEGLQSKLERWRTELPPVYDRGELSSFPP 480
           SPSEVAAQMATQK+DD NESSIVGGLSLNDSVEGLQSKLERWRTELPPVYDRGELSS P 
Sbjct: 447 SPSEVAAQMATQKIDDGNESSIVGGLSLNDSVEGLQSKLERWRTELPPVYDRGELSSLPT 506

Query: 481 SESR-HNRRHSDGGGLFSCFSNICGCECSIVCGGGSQKAPKKSPSGRVGRSPSADNVSFM 537
           SE+R HNRRHSDGGGLFSCFSNICGCECSIVC GGS KAPKKSPSGRV RSPSAD VSF+
Sbjct: 507 SETRQHNRRHSDGGGLFSCFSNICGCECSIVC-GGSPKAPKKSPSGRVVRSPSADQVSFI 557

BLAST of Spg023271 vs. NCBI nr
Match: XP_004147189.1 (uncharacterized protein LOC101215059 [Cucumis sativus] >KGN61560.1 hypothetical protein Csa_006811 [Cucumis sativus])

HSP 1 Score: 894.0 bits (2309), Expect = 6.2e-256
Identity = 475/539 (88.13%), Postives = 497/539 (92.21%), Query Frame = 0

Query: 1   MRTYAVAWVHPDRKLSTRVDTHGHNNPTWNDKFVFRVDDEFLHADTSAVMIEIYALHWFK 60
           MRTYAVAWVHPDRKLSTRVDTHGHNNPTWNDKFVFRVDDEFLH+DTSAVMIEIYALHWFK
Sbjct: 27  MRTYAVAWVHPDRKLSTRVDTHGHNNPTWNDKFVFRVDDEFLHSDTSAVMIEIYALHWFK 86

Query: 61  DIHVGTVRILVGNLIPTPPRLHQFSQQPQVGMRFVALQVRRPSGRPQGILNIGVALLHSS 120
           DIHVGTVRILVGNLIPTPPRLHQFSQQPQVGMRFVALQVRRPSGRPQGILNIGVALLHSS
Sbjct: 87  DIHVGTVRILVGNLIPTPPRLHQFSQQPQVGMRFVALQVRRPSGRPQGILNIGVALLHSS 146

Query: 121 MRSMPLYSQLSTSAIGYRNLMGEEDPFHNHHSNTNNHSHGTPTVVTRPEMRRSKSDTSSM 180
           MRSMPLYSQLSTSAIGYRNLMGEEDPF NHHSNTNN SHGTPTV+TRPEMRRSKSDTSSM
Sbjct: 147 MRSMPLYSQLSTSAIGYRNLMGEEDPFANHHSNTNNQSHGTPTVLTRPEMRRSKSDTSSM 206

Query: 181 MGSELRGSEMMGKGMKSKASSMINGSEVSKHKKKGRSKASSMISGSVVIKRKGKSRKASS 240
           +GSELRGSEMMGKGMKSKASSM+NGSEVSK KKKGRSKASSMISGSVVIKRKGKSRKASS
Sbjct: 207 IGSELRGSEMMGKGMKSKASSMVNGSEVSKQKKKGRSKASSMISGSVVIKRKGKSRKASS 266

Query: 241 MLSGSGIEEAYNKQNGKNGKSNAVIVGKKEKTEPSSILSGSEVYPIELPPNKKETPVHKP 300
           M+SGSGIEE Y  +NGKNGKSN  IVGKK+K + SSILSGSEVYP+E   NKKE PV K 
Sbjct: 267 MISGSGIEEGYAVKNGKNGKSNPGIVGKKDKVDQSSILSGSEVYPVEQLSNKKEKPVQKL 326

Query: 301 NSSEETFDSPPWKK-PQEKPISRPEVTDSPRGKPGQETPNSRFDKMPYATPLRTPQRLAG 360
           NSSEE+F+S  WKK P E P S+ EV DSPRGKPGQETPNSRFDKMPYATPLRTPQRLA 
Sbjct: 327 NSSEESFNSQSWKKPPNEIPNSKVEVVDSPRGKPGQETPNSRFDKMPYATPLRTPQRLA- 386

Query: 361 GGGTARATPMHEKFATPRRTPTPLHGKSSHTKYNNDFNYTTPRKSFLGGAILTESELGPS 420
           G GTARATPMH+KFATPRR+ TPL+GKSS+TK+ N+F YTTPRKSFLGGA LTESELGPS
Sbjct: 387 GSGTARATPMHDKFATPRRS-TPLNGKSSYTKH-NEFTYTTPRKSFLGGAFLTESELGPS 446

Query: 421 PSEVAAQMATQKVDD-NESSIVGGLSLNDSVEGLQSKLERWRTELPPVYDRGELSSFPPS 480
           PSEVAAQMA QK +D NESSIVGGLSLNDSVEGLQSKLERWRTELPPVYDRGELSSFP S
Sbjct: 447 PSEVAAQMARQKTEDGNESSIVGGLSLNDSVEGLQSKLERWRTELPPVYDRGELSSFPSS 506

Query: 481 ESR-HNRRHSDGGGLFSCFSNICGCECSIVCGGGSQKAPKKSPSGRVGRSPSADNVSFM 537
           E+R HNRRHSDGGGLFSCFSNICGCECSIVC GGS KA KKS SGR+ RSPS D VSF+
Sbjct: 507 ETREHNRRHSDGGGLFSCFSNICGCECSIVC-GGSPKATKKSASGRLVRSPSVDQVSFL 561

BLAST of Spg023271 vs. NCBI nr
Match: XP_008460692.1 (PREDICTED: uncharacterized protein LOC103499460 [Cucumis melo] >KAA0031939.1 C2 domain-containing family protein [Cucumis melo var. makuwa] >TYK16760.1 C2 domain-containing family protein [Cucumis melo var. makuwa])

HSP 1 Score: 887.9 bits (2293), Expect = 4.4e-254
Identity = 471/539 (87.38%), Postives = 497/539 (92.21%), Query Frame = 0

Query: 1   MRTYAVAWVHPDRKLSTRVDTHGHNNPTWNDKFVFRVDDEFLHADTSAVMIEIYALHWFK 60
           MRTYAVAWVHPDRKLSTRVDTHGHNNPTWNDKFVFRVDDEFLH+DTSAVMIEIYALHWFK
Sbjct: 27  MRTYAVAWVHPDRKLSTRVDTHGHNNPTWNDKFVFRVDDEFLHSDTSAVMIEIYALHWFK 86

Query: 61  DIHVGTVRILVGNLIPTPPRLHQFSQQPQVGMRFVALQVRRPSGRPQGILNIGVALLHSS 120
           DIHVGTVRILVGNLIPTPPRLHQFSQQPQVGMRFVALQVRRPSGRPQGILNIGVALLHSS
Sbjct: 87  DIHVGTVRILVGNLIPTPPRLHQFSQQPQVGMRFVALQVRRPSGRPQGILNIGVALLHSS 146

Query: 121 MRSMPLYSQLSTSAIGYRNLMGEEDPFHNHHSNTNNHSHGTPTVVTRPEMRRSKSDTSSM 180
           MRSMPLYSQLSTSAIGYRNLMGEEDPF  HHS  NN SHGTPTV+TRPEMRRSKSDTSSM
Sbjct: 147 MRSMPLYSQLSTSAIGYRNLMGEEDPFATHHSTNNNQSHGTPTVLTRPEMRRSKSDTSSM 206

Query: 181 MGSELRGSEMMGKGMKSKASSMINGSEVSKHKKKGRSKASSMISGSVVIKRKGKSRKASS 240
           +GSELRGSEMMGKG+KSKASSM+NGSEVSK KKKGRSKASSMISGSVVIKRKGK+RKASS
Sbjct: 207 IGSELRGSEMMGKGLKSKASSMVNGSEVSKQKKKGRSKASSMISGSVVIKRKGKTRKASS 266

Query: 241 MLSGSGIEEAYNKQNGKNGKSNAVIVGKKEKTEPSSILSGSEVYPIELPPNKKETPVHKP 300
           M+SGSGIEE    +NGKNGKS+ +IVGKK+K + SSILSGSEVYPIE P NKKE PV K 
Sbjct: 267 MISGSGIEEGCTVKNGKNGKSSPLIVGKKDKVDQSSILSGSEVYPIEPPSNKKEKPVQKV 326

Query: 301 NSSEETFDSPPWKKP-QEKPISRPEVTDSPRGKPGQETPNSRFDKMPYATPLRTPQRLAG 360
           +SSEE+F+S P+KKP  +K  ++ EV DSPRGK GQETPNSRFDKMPYATPLRTPQRLA 
Sbjct: 327 DSSEESFNSQPYKKPLHDKTNTKHEVADSPRGKLGQETPNSRFDKMPYATPLRTPQRLA- 386

Query: 361 GGGTARATPMHEKFATPRRTPTPLHGKSSHTKYNNDFNYTTPRKSFLGGAILTESELGPS 420
           GGGTARATPMH+KFATPRR+ TPL GKSS+TKY N+F YTTPRKSFLGGA+LTESELGPS
Sbjct: 387 GGGTARATPMHDKFATPRRS-TPLQGKSSYTKY-NEFTYTTPRKSFLGGAVLTESELGPS 446

Query: 421 PSEVAAQMATQKVDD-NESSIVGGLSLNDSVEGLQSKLERWRTELPPVYDRGELSSFPPS 480
           PSEVAAQMA QK+DD NESSIVGGLSLNDSVEGLQSKLERWRTELPPVYDRGELSSFP S
Sbjct: 447 PSEVAAQMAKQKIDDGNESSIVGGLSLNDSVEGLQSKLERWRTELPPVYDRGELSSFPSS 506

Query: 481 ESR-HNRRHSDGGGLFSCFSNICGCECSIVCGGGSQKAPKKSPSGRVGRSPSADNVSFM 537
           E+R HNRRHSDGGGLFSCFSNICGCECSIVC GGS KA KKS SGRV RSPS D VSFM
Sbjct: 507 ETRQHNRRHSDGGGLFSCFSNICGCECSIVC-GGSPKATKKSSSGRVVRSPSVDQVSFM 561

BLAST of Spg023271 vs. NCBI nr
Match: XP_022959100.1 (uncharacterized protein LOC111460196 [Cucurbita moschata])

HSP 1 Score: 773.1 bits (1995), Expect = 1.6e-219
Identity = 422/540 (78.15%), Postives = 457/540 (84.63%), Query Frame = 0

Query: 1   MRTYAVAWVHPDRKLSTRVDTHGHNNPTWNDKFVFRVDDEFLHADTSAVMIEIYALHWFK 60
           MRTYA+AWVHPDR+LSTRVDTHGHNNPTWNDKFVFRVDDEFLH+DTSAVMIEIYALHWFK
Sbjct: 27  MRTYAIAWVHPDRRLSTRVDTHGHNNPTWNDKFVFRVDDEFLHSDTSAVMIEIYALHWFK 86

Query: 61  DIHVGTVRILVGNLIPTPPRLHQFSQQPQVGMRFVALQVRRPSGRPQGILNIGVALLHSS 120
           DIHVGTVRILVGNL PTPPRLHQFSQQPQVGMRFVALQVRRPSGRPQGILNIGVALLHSS
Sbjct: 87  DIHVGTVRILVGNLFPTPPRLHQFSQQPQVGMRFVALQVRRPSGRPQGILNIGVALLHSS 146

Query: 121 MRSMPLYSQLSTSAIGYRNLMGEEDPFHNHHSNTNNHSHGTPTVVTRPEMRRSKSDTSSM 180
           MRSMPLYSQLSTSAIGYRNLMGEEDPF NH        HGTPTVVTRPE RRSKSD SSM
Sbjct: 147 MRSMPLYSQLSTSAIGYRNLMGEEDPFRNH--------HGTPTVVTRPEFRRSKSDISSM 206

Query: 181 MGSELRGSEMMGKGMKSKASSMINGSEVS-KHKKKGRSKASSMISGSVVIKRKGKSRKAS 240
           +GS +RGSEM+GKG KS+ASSM+NGSE++ K+KKKGRSK+SSMISGS+ I+R+ K RKA+
Sbjct: 207 VGSTVRGSEMLGKGTKSRASSMVNGSEITRKNKKKGRSKSSSMISGSITIRRREKPRKAN 266

Query: 241 SMLSGSGIEEAYNKQNGKNGKSNAVIVGKKEKTEPSSILSGSEVYPIELPPNKKETPVHK 300
           SM+SG                S++ +V K+EK + SSILSGSEVYP E PP K   PV K
Sbjct: 267 SMISG----------------SSSAMVTKREKIKQSSILSGSEVYP-EPPPEK---PVQK 326

Query: 301 PNSSEETFDSPPWKKP-QEKPISRPEVTDSPRGKPGQETPNSRFDKMPYATPLRTPQRL- 360
            N    T DSP WKKP QEKPIS          K GQETPNSRFDKMPYATPLRTPQRL 
Sbjct: 327 MN----TGDSPAWKKPSQEKPIS----------KHGQETPNSRFDKMPYATPLRTPQRLT 386

Query: 361 AGGGGTARATPMHEKFATPRRTPTPLHGKSSHTKYNNDFNYTTPRKSFLGGAILTESELG 420
            GGGGTARATPMHEK+ATPRR+ TP++GKSS+ K+ N+F YTTPRKSFLGGA LTESELG
Sbjct: 387 GGGGGTARATPMHEKYATPRRS-TPMNGKSSYHKH-NEFTYTTPRKSFLGGAFLTESELG 446

Query: 421 PSPSEVAAQMATQKVDD-NESSIVGGLSLNDSVEGLQSKLERWRTELPPVYDRGELSSFP 480
           PSPSEVAAQMATQK DD NESSIVGGLSLNDS+EGLQSKLERWRTELPPVYDRGE++SFP
Sbjct: 447 PSPSEVAAQMATQKTDDSNESSIVGGLSLNDSIEGLQSKLERWRTELPPVYDRGEITSFP 506

Query: 481 PSESRHNRRHSDGGGLFSCFSNICGCECSIVCGGGSQKAPKKSPSGRVGRSPSADNVSFM 537
           PSE    RRHSDGGGLFSCFSNICGCECSIVC GGS K PKK P+GRV RSPSA ++SFM
Sbjct: 507 PSEDHPERRHSDGGGLFSCFSNICGCECSIVC-GGSPKTPKKPPNGRVTRSPSAGHLSFM 521

BLAST of Spg023271 vs. NCBI nr
Match: KAG6574889.1 (hypothetical protein SDJN03_25528, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013461.1 hypothetical protein SDJN02_23627, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 770.8 bits (1989), Expect = 7.9e-219
Identity = 423/540 (78.33%), Postives = 456/540 (84.44%), Query Frame = 0

Query: 1   MRTYAVAWVHPDRKLSTRVDTHGHNNPTWNDKFVFRVDDEFLHADTSAVMIEIYALHWFK 60
           MRTYA+AWVHPDRKLSTRVDTHGHNNPTWNDKFVFRVDDEFLH+DTSAVMIEIYALHWFK
Sbjct: 27  MRTYAIAWVHPDRKLSTRVDTHGHNNPTWNDKFVFRVDDEFLHSDTSAVMIEIYALHWFK 86

Query: 61  DIHVGTVRILVGNLIPTPPRLHQFSQQPQVGMRFVALQVRRPSGRPQGILNIGVALLHSS 120
           DIHVGTVRILVGNL PTPPRLHQFSQQPQVGMRFVALQVRRPSGRPQGILNIGVALLHSS
Sbjct: 87  DIHVGTVRILVGNLFPTPPRLHQFSQQPQVGMRFVALQVRRPSGRPQGILNIGVALLHSS 146

Query: 121 MRSMPLYSQLSTSAIGYRNLMGEEDPFHNHHSNTNNHSHGTPTVVTRPEMRRSKSDTSSM 180
           MRSMPLYSQLSTSAIGYRNLMGEEDPF NH        HGTPTVVTRPE RRSKSD SSM
Sbjct: 147 MRSMPLYSQLSTSAIGYRNLMGEEDPFRNH--------HGTPTVVTRPEFRRSKSDISSM 206

Query: 181 MGSELRGSEMMGKGMKSKASSMINGSEVS-KHKKKGRSKASSMISGSVVIKRKGKSRKAS 240
           +GS +RGSEM+GKG KS+ASSM+NGSE++ K+KKKGRSK+SSMISGS+ I+R+ K RKA+
Sbjct: 207 VGSTVRGSEMLGKGTKSRASSMVNGSEITRKNKKKGRSKSSSMISGSITIRRREKPRKAN 266

Query: 241 SMLSGSGIEEAYNKQNGKNGKSNAVIVGKKEKTEPSSILSGSEVYPIELPPNKKETPVHK 300
           SM+SG                S++V+V K+EK + SSILSGSEVYP E PP K   PV K
Sbjct: 267 SMISG----------------SSSVMVTKREKIKQSSILSGSEVYP-EPPPEK---PVQK 326

Query: 301 PNSSEETFDSPPWKKP-QEKPISRPEVTDSPRGKPGQETPNSRFDKMPYATPLRTPQRL- 360
            N    T DSP WKKP QEKPIS          K GQETPNSRFDKMPYATPLRTPQRL 
Sbjct: 327 MN----TGDSPAWKKPSQEKPIS----------KHGQETPNSRFDKMPYATPLRTPQRLT 386

Query: 361 AGGGGTARATPMHEKFATPRRTPTPLHGKSSHTKYNNDFNYTTPRKSFLGGAILTESELG 420
            GGGGTARATPMHEK+ATPRR+ TP++GKSS+ K+ N+F YTTPRKSFLGGA LTESELG
Sbjct: 387 GGGGGTARATPMHEKYATPRRS-TPMNGKSSYHKH-NEFTYTTPRKSFLGGAFLTESELG 446

Query: 421 PSPSEVAAQMATQKVDD-NESSIVGGLSLNDSVEGLQSKLERWRTELPPVYDRGELSSFP 480
           PSPSEVAAQMATQK DD NESSIVG LSLNDSVEGLQSKLERWRTELPPVYDRGE++SFP
Sbjct: 447 PSPSEVAAQMATQKTDDSNESSIVGALSLNDSVEGLQSKLERWRTELPPVYDRGEITSFP 506

Query: 481 PSESRHNRRHSDGGGLFSCFSNICGCECSIVCGGGSQKAPKKSPSGRVGRSPSADNVSFM 537
           PSE    RRHSDGGGLFSCFSNICGCECSIVC GGS K PKK  +GRV RSPSA ++SFM
Sbjct: 507 PSEDHPERRHSDGGGLFSCFSNICGCECSIVC-GGSPKTPKKPANGRVTRSPSAGHLSFM 521

BLAST of Spg023271 vs. ExPASy TrEMBL
Match: A0A0A0LI48 (C2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G171820 PE=4 SV=1)

HSP 1 Score: 894.0 bits (2309), Expect = 3.0e-256
Identity = 475/539 (88.13%), Postives = 497/539 (92.21%), Query Frame = 0

Query: 1   MRTYAVAWVHPDRKLSTRVDTHGHNNPTWNDKFVFRVDDEFLHADTSAVMIEIYALHWFK 60
           MRTYAVAWVHPDRKLSTRVDTHGHNNPTWNDKFVFRVDDEFLH+DTSAVMIEIYALHWFK
Sbjct: 27  MRTYAVAWVHPDRKLSTRVDTHGHNNPTWNDKFVFRVDDEFLHSDTSAVMIEIYALHWFK 86

Query: 61  DIHVGTVRILVGNLIPTPPRLHQFSQQPQVGMRFVALQVRRPSGRPQGILNIGVALLHSS 120
           DIHVGTVRILVGNLIPTPPRLHQFSQQPQVGMRFVALQVRRPSGRPQGILNIGVALLHSS
Sbjct: 87  DIHVGTVRILVGNLIPTPPRLHQFSQQPQVGMRFVALQVRRPSGRPQGILNIGVALLHSS 146

Query: 121 MRSMPLYSQLSTSAIGYRNLMGEEDPFHNHHSNTNNHSHGTPTVVTRPEMRRSKSDTSSM 180
           MRSMPLYSQLSTSAIGYRNLMGEEDPF NHHSNTNN SHGTPTV+TRPEMRRSKSDTSSM
Sbjct: 147 MRSMPLYSQLSTSAIGYRNLMGEEDPFANHHSNTNNQSHGTPTVLTRPEMRRSKSDTSSM 206

Query: 181 MGSELRGSEMMGKGMKSKASSMINGSEVSKHKKKGRSKASSMISGSVVIKRKGKSRKASS 240
           +GSELRGSEMMGKGMKSKASSM+NGSEVSK KKKGRSKASSMISGSVVIKRKGKSRKASS
Sbjct: 207 IGSELRGSEMMGKGMKSKASSMVNGSEVSKQKKKGRSKASSMISGSVVIKRKGKSRKASS 266

Query: 241 MLSGSGIEEAYNKQNGKNGKSNAVIVGKKEKTEPSSILSGSEVYPIELPPNKKETPVHKP 300
           M+SGSGIEE Y  +NGKNGKSN  IVGKK+K + SSILSGSEVYP+E   NKKE PV K 
Sbjct: 267 MISGSGIEEGYAVKNGKNGKSNPGIVGKKDKVDQSSILSGSEVYPVEQLSNKKEKPVQKL 326

Query: 301 NSSEETFDSPPWKK-PQEKPISRPEVTDSPRGKPGQETPNSRFDKMPYATPLRTPQRLAG 360
           NSSEE+F+S  WKK P E P S+ EV DSPRGKPGQETPNSRFDKMPYATPLRTPQRLA 
Sbjct: 327 NSSEESFNSQSWKKPPNEIPNSKVEVVDSPRGKPGQETPNSRFDKMPYATPLRTPQRLA- 386

Query: 361 GGGTARATPMHEKFATPRRTPTPLHGKSSHTKYNNDFNYTTPRKSFLGGAILTESELGPS 420
           G GTARATPMH+KFATPRR+ TPL+GKSS+TK+ N+F YTTPRKSFLGGA LTESELGPS
Sbjct: 387 GSGTARATPMHDKFATPRRS-TPLNGKSSYTKH-NEFTYTTPRKSFLGGAFLTESELGPS 446

Query: 421 PSEVAAQMATQKVDD-NESSIVGGLSLNDSVEGLQSKLERWRTELPPVYDRGELSSFPPS 480
           PSEVAAQMA QK +D NESSIVGGLSLNDSVEGLQSKLERWRTELPPVYDRGELSSFP S
Sbjct: 447 PSEVAAQMARQKTEDGNESSIVGGLSLNDSVEGLQSKLERWRTELPPVYDRGELSSFPSS 506

Query: 481 ESR-HNRRHSDGGGLFSCFSNICGCECSIVCGGGSQKAPKKSPSGRVGRSPSADNVSFM 537
           E+R HNRRHSDGGGLFSCFSNICGCECSIVC GGS KA KKS SGR+ RSPS D VSF+
Sbjct: 507 ETREHNRRHSDGGGLFSCFSNICGCECSIVC-GGSPKATKKSASGRLVRSPSVDQVSFL 561

BLAST of Spg023271 vs. ExPASy TrEMBL
Match: A0A5D3CZG7 (C2 domain-containing family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold96G00130 PE=4 SV=1)

HSP 1 Score: 887.9 bits (2293), Expect = 2.1e-254
Identity = 471/539 (87.38%), Postives = 497/539 (92.21%), Query Frame = 0

Query: 1   MRTYAVAWVHPDRKLSTRVDTHGHNNPTWNDKFVFRVDDEFLHADTSAVMIEIYALHWFK 60
           MRTYAVAWVHPDRKLSTRVDTHGHNNPTWNDKFVFRVDDEFLH+DTSAVMIEIYALHWFK
Sbjct: 27  MRTYAVAWVHPDRKLSTRVDTHGHNNPTWNDKFVFRVDDEFLHSDTSAVMIEIYALHWFK 86

Query: 61  DIHVGTVRILVGNLIPTPPRLHQFSQQPQVGMRFVALQVRRPSGRPQGILNIGVALLHSS 120
           DIHVGTVRILVGNLIPTPPRLHQFSQQPQVGMRFVALQVRRPSGRPQGILNIGVALLHSS
Sbjct: 87  DIHVGTVRILVGNLIPTPPRLHQFSQQPQVGMRFVALQVRRPSGRPQGILNIGVALLHSS 146

Query: 121 MRSMPLYSQLSTSAIGYRNLMGEEDPFHNHHSNTNNHSHGTPTVVTRPEMRRSKSDTSSM 180
           MRSMPLYSQLSTSAIGYRNLMGEEDPF  HHS  NN SHGTPTV+TRPEMRRSKSDTSSM
Sbjct: 147 MRSMPLYSQLSTSAIGYRNLMGEEDPFATHHSTNNNQSHGTPTVLTRPEMRRSKSDTSSM 206

Query: 181 MGSELRGSEMMGKGMKSKASSMINGSEVSKHKKKGRSKASSMISGSVVIKRKGKSRKASS 240
           +GSELRGSEMMGKG+KSKASSM+NGSEVSK KKKGRSKASSMISGSVVIKRKGK+RKASS
Sbjct: 207 IGSELRGSEMMGKGLKSKASSMVNGSEVSKQKKKGRSKASSMISGSVVIKRKGKTRKASS 266

Query: 241 MLSGSGIEEAYNKQNGKNGKSNAVIVGKKEKTEPSSILSGSEVYPIELPPNKKETPVHKP 300
           M+SGSGIEE    +NGKNGKS+ +IVGKK+K + SSILSGSEVYPIE P NKKE PV K 
Sbjct: 267 MISGSGIEEGCTVKNGKNGKSSPLIVGKKDKVDQSSILSGSEVYPIEPPSNKKEKPVQKV 326

Query: 301 NSSEETFDSPPWKKP-QEKPISRPEVTDSPRGKPGQETPNSRFDKMPYATPLRTPQRLAG 360
           +SSEE+F+S P+KKP  +K  ++ EV DSPRGK GQETPNSRFDKMPYATPLRTPQRLA 
Sbjct: 327 DSSEESFNSQPYKKPLHDKTNTKHEVADSPRGKLGQETPNSRFDKMPYATPLRTPQRLA- 386

Query: 361 GGGTARATPMHEKFATPRRTPTPLHGKSSHTKYNNDFNYTTPRKSFLGGAILTESELGPS 420
           GGGTARATPMH+KFATPRR+ TPL GKSS+TKY N+F YTTPRKSFLGGA+LTESELGPS
Sbjct: 387 GGGTARATPMHDKFATPRRS-TPLQGKSSYTKY-NEFTYTTPRKSFLGGAVLTESELGPS 446

Query: 421 PSEVAAQMATQKVDD-NESSIVGGLSLNDSVEGLQSKLERWRTELPPVYDRGELSSFPPS 480
           PSEVAAQMA QK+DD NESSIVGGLSLNDSVEGLQSKLERWRTELPPVYDRGELSSFP S
Sbjct: 447 PSEVAAQMAKQKIDDGNESSIVGGLSLNDSVEGLQSKLERWRTELPPVYDRGELSSFPSS 506

Query: 481 ESR-HNRRHSDGGGLFSCFSNICGCECSIVCGGGSQKAPKKSPSGRVGRSPSADNVSFM 537
           E+R HNRRHSDGGGLFSCFSNICGCECSIVC GGS KA KKS SGRV RSPS D VSFM
Sbjct: 507 ETRQHNRRHSDGGGLFSCFSNICGCECSIVC-GGSPKATKKSSSGRVVRSPSVDQVSFM 561

BLAST of Spg023271 vs. ExPASy TrEMBL
Match: A0A1S3CE80 (uncharacterized protein LOC103499460 OS=Cucumis melo OX=3656 GN=LOC103499460 PE=4 SV=1)

HSP 1 Score: 887.9 bits (2293), Expect = 2.1e-254
Identity = 471/539 (87.38%), Postives = 497/539 (92.21%), Query Frame = 0

Query: 1   MRTYAVAWVHPDRKLSTRVDTHGHNNPTWNDKFVFRVDDEFLHADTSAVMIEIYALHWFK 60
           MRTYAVAWVHPDRKLSTRVDTHGHNNPTWNDKFVFRVDDEFLH+DTSAVMIEIYALHWFK
Sbjct: 27  MRTYAVAWVHPDRKLSTRVDTHGHNNPTWNDKFVFRVDDEFLHSDTSAVMIEIYALHWFK 86

Query: 61  DIHVGTVRILVGNLIPTPPRLHQFSQQPQVGMRFVALQVRRPSGRPQGILNIGVALLHSS 120
           DIHVGTVRILVGNLIPTPPRLHQFSQQPQVGMRFVALQVRRPSGRPQGILNIGVALLHSS
Sbjct: 87  DIHVGTVRILVGNLIPTPPRLHQFSQQPQVGMRFVALQVRRPSGRPQGILNIGVALLHSS 146

Query: 121 MRSMPLYSQLSTSAIGYRNLMGEEDPFHNHHSNTNNHSHGTPTVVTRPEMRRSKSDTSSM 180
           MRSMPLYSQLSTSAIGYRNLMGEEDPF  HHS  NN SHGTPTV+TRPEMRRSKSDTSSM
Sbjct: 147 MRSMPLYSQLSTSAIGYRNLMGEEDPFATHHSTNNNQSHGTPTVLTRPEMRRSKSDTSSM 206

Query: 181 MGSELRGSEMMGKGMKSKASSMINGSEVSKHKKKGRSKASSMISGSVVIKRKGKSRKASS 240
           +GSELRGSEMMGKG+KSKASSM+NGSEVSK KKKGRSKASSMISGSVVIKRKGK+RKASS
Sbjct: 207 IGSELRGSEMMGKGLKSKASSMVNGSEVSKQKKKGRSKASSMISGSVVIKRKGKTRKASS 266

Query: 241 MLSGSGIEEAYNKQNGKNGKSNAVIVGKKEKTEPSSILSGSEVYPIELPPNKKETPVHKP 300
           M+SGSGIEE    +NGKNGKS+ +IVGKK+K + SSILSGSEVYPIE P NKKE PV K 
Sbjct: 267 MISGSGIEEGCTVKNGKNGKSSPLIVGKKDKVDQSSILSGSEVYPIEPPSNKKEKPVQKV 326

Query: 301 NSSEETFDSPPWKKP-QEKPISRPEVTDSPRGKPGQETPNSRFDKMPYATPLRTPQRLAG 360
           +SSEE+F+S P+KKP  +K  ++ EV DSPRGK GQETPNSRFDKMPYATPLRTPQRLA 
Sbjct: 327 DSSEESFNSQPYKKPLHDKTNTKHEVADSPRGKLGQETPNSRFDKMPYATPLRTPQRLA- 386

Query: 361 GGGTARATPMHEKFATPRRTPTPLHGKSSHTKYNNDFNYTTPRKSFLGGAILTESELGPS 420
           GGGTARATPMH+KFATPRR+ TPL GKSS+TKY N+F YTTPRKSFLGGA+LTESELGPS
Sbjct: 387 GGGTARATPMHDKFATPRRS-TPLQGKSSYTKY-NEFTYTTPRKSFLGGAVLTESELGPS 446

Query: 421 PSEVAAQMATQKVDD-NESSIVGGLSLNDSVEGLQSKLERWRTELPPVYDRGELSSFPPS 480
           PSEVAAQMA QK+DD NESSIVGGLSLNDSVEGLQSKLERWRTELPPVYDRGELSSFP S
Sbjct: 447 PSEVAAQMAKQKIDDGNESSIVGGLSLNDSVEGLQSKLERWRTELPPVYDRGELSSFPSS 506

Query: 481 ESR-HNRRHSDGGGLFSCFSNICGCECSIVCGGGSQKAPKKSPSGRVGRSPSADNVSFM 537
           E+R HNRRHSDGGGLFSCFSNICGCECSIVC GGS KA KKS SGRV RSPS D VSFM
Sbjct: 507 ETRQHNRRHSDGGGLFSCFSNICGCECSIVC-GGSPKATKKSSSGRVVRSPSVDQVSFM 561

BLAST of Spg023271 vs. ExPASy TrEMBL
Match: A0A6J1H5C1 (uncharacterized protein LOC111460196 OS=Cucurbita moschata OX=3662 GN=LOC111460196 PE=4 SV=1)

HSP 1 Score: 773.1 bits (1995), Expect = 7.7e-220
Identity = 422/540 (78.15%), Postives = 457/540 (84.63%), Query Frame = 0

Query: 1   MRTYAVAWVHPDRKLSTRVDTHGHNNPTWNDKFVFRVDDEFLHADTSAVMIEIYALHWFK 60
           MRTYA+AWVHPDR+LSTRVDTHGHNNPTWNDKFVFRVDDEFLH+DTSAVMIEIYALHWFK
Sbjct: 27  MRTYAIAWVHPDRRLSTRVDTHGHNNPTWNDKFVFRVDDEFLHSDTSAVMIEIYALHWFK 86

Query: 61  DIHVGTVRILVGNLIPTPPRLHQFSQQPQVGMRFVALQVRRPSGRPQGILNIGVALLHSS 120
           DIHVGTVRILVGNL PTPPRLHQFSQQPQVGMRFVALQVRRPSGRPQGILNIGVALLHSS
Sbjct: 87  DIHVGTVRILVGNLFPTPPRLHQFSQQPQVGMRFVALQVRRPSGRPQGILNIGVALLHSS 146

Query: 121 MRSMPLYSQLSTSAIGYRNLMGEEDPFHNHHSNTNNHSHGTPTVVTRPEMRRSKSDTSSM 180
           MRSMPLYSQLSTSAIGYRNLMGEEDPF NH        HGTPTVVTRPE RRSKSD SSM
Sbjct: 147 MRSMPLYSQLSTSAIGYRNLMGEEDPFRNH--------HGTPTVVTRPEFRRSKSDISSM 206

Query: 181 MGSELRGSEMMGKGMKSKASSMINGSEVS-KHKKKGRSKASSMISGSVVIKRKGKSRKAS 240
           +GS +RGSEM+GKG KS+ASSM+NGSE++ K+KKKGRSK+SSMISGS+ I+R+ K RKA+
Sbjct: 207 VGSTVRGSEMLGKGTKSRASSMVNGSEITRKNKKKGRSKSSSMISGSITIRRREKPRKAN 266

Query: 241 SMLSGSGIEEAYNKQNGKNGKSNAVIVGKKEKTEPSSILSGSEVYPIELPPNKKETPVHK 300
           SM+SG                S++ +V K+EK + SSILSGSEVYP E PP K   PV K
Sbjct: 267 SMISG----------------SSSAMVTKREKIKQSSILSGSEVYP-EPPPEK---PVQK 326

Query: 301 PNSSEETFDSPPWKKP-QEKPISRPEVTDSPRGKPGQETPNSRFDKMPYATPLRTPQRL- 360
            N    T DSP WKKP QEKPIS          K GQETPNSRFDKMPYATPLRTPQRL 
Sbjct: 327 MN----TGDSPAWKKPSQEKPIS----------KHGQETPNSRFDKMPYATPLRTPQRLT 386

Query: 361 AGGGGTARATPMHEKFATPRRTPTPLHGKSSHTKYNNDFNYTTPRKSFLGGAILTESELG 420
            GGGGTARATPMHEK+ATPRR+ TP++GKSS+ K+ N+F YTTPRKSFLGGA LTESELG
Sbjct: 387 GGGGGTARATPMHEKYATPRRS-TPMNGKSSYHKH-NEFTYTTPRKSFLGGAFLTESELG 446

Query: 421 PSPSEVAAQMATQKVDD-NESSIVGGLSLNDSVEGLQSKLERWRTELPPVYDRGELSSFP 480
           PSPSEVAAQMATQK DD NESSIVGGLSLNDS+EGLQSKLERWRTELPPVYDRGE++SFP
Sbjct: 447 PSPSEVAAQMATQKTDDSNESSIVGGLSLNDSIEGLQSKLERWRTELPPVYDRGEITSFP 506

Query: 481 PSESRHNRRHSDGGGLFSCFSNICGCECSIVCGGGSQKAPKKSPSGRVGRSPSADNVSFM 537
           PSE    RRHSDGGGLFSCFSNICGCECSIVC GGS K PKK P+GRV RSPSA ++SFM
Sbjct: 507 PSEDHPERRHSDGGGLFSCFSNICGCECSIVC-GGSPKTPKKPPNGRVTRSPSAGHLSFM 521

BLAST of Spg023271 vs. ExPASy TrEMBL
Match: A0A6J1KG00 (uncharacterized protein LOC111494746 OS=Cucurbita maxima OX=3661 GN=LOC111494746 PE=4 SV=1)

HSP 1 Score: 754.2 bits (1946), Expect = 3.7e-214
Identity = 416/537 (77.47%), Postives = 442/537 (82.31%), Query Frame = 0

Query: 1   MRTYAVAWVHPDRKLSTRVDTHGHNNPTWNDKFVFRVDDEFLHADTSAVMIEIYALHWFK 60
           MRTYAVAWVHPDRKLSTRVDTHGHNNPTWNDKFVFRVDDEFLHADTSAVMIEIYALHWFK
Sbjct: 27  MRTYAVAWVHPDRKLSTRVDTHGHNNPTWNDKFVFRVDDEFLHADTSAVMIEIYALHWFK 86

Query: 61  DIHVGTVRILVGNLIPTPPRLHQFSQQPQVGMRFVALQVRRPSGRPQGILNIGVALLHSS 120
           DIHVGTVRILVGNLIPT PRLHQFSQQPQVGMRFVALQ RRPSGRPQGILNIGVALLHSS
Sbjct: 87  DIHVGTVRILVGNLIPTQPRLHQFSQQPQVGMRFVALQARRPSGRPQGILNIGVALLHSS 146

Query: 121 MRSMPLYSQLSTSAIGYRNLMGEEDPFHNHHSNTNNHSHGTPTVVTRPEMRRSKSDTSSM 180
           MRSMPLYSQLSTSAIGYR+LMGEED FH      NN SHGTPT  T P+MRRSKSDTSSM
Sbjct: 147 MRSMPLYSQLSTSAIGYRDLMGEEDAFH------NNRSHGTPTATTLPKMRRSKSDTSSM 206

Query: 181 MGSELRGSEMMGKGMKSKASSMINGSEVSKHKKKGRSKASSMISGSVVIKRKGKSRKASS 240
           MGSELRGSE+MGK  KSKASSM++GSE+SK KKKGRSKASSMISGSV IKRK KS+KASS
Sbjct: 207 MGSELRGSEIMGKTTKSKASSMVDGSEISKTKKKGRSKASSMISGSVGIKRKEKSKKASS 266

Query: 241 MLSGSGIEEAYNKQNGKNGKSNAVIVGKKEKTEPSSILSGSEVYPIELPP-NKKETPVHK 300
           MLSGSGIEEAYNK+             K EK  P ++ + ++VY IE PP +KKET   K
Sbjct: 267 MLSGSGIEEAYNKK-------------KTEKPNPFNV-ANNKVYKIEPPPSSKKET---K 326

Query: 301 PNSSEETFDSPPWKKPQEKPISRPEVTDSPRGKPGQETPNSRFDKMPYATPLRTPQRLAG 360
           PNS              EKPIS  E  DSPR  PG ETPN R+DKMPYATPLRTP+RL G
Sbjct: 327 PNSG------------YEKPISILEFIDSPRIHPGLETPNPRYDKMPYATPLRTPRRLTG 386

Query: 361 GGGTARATPMHEKFATPRRTPTPLHGKSSHTKYNNDFNYTTPRKSFLGGAILTESELGPS 420
           GGGTA+ TPMHE   TPRR  TPL G+SS TK NN+F YTTPRKS L GA  TESELGPS
Sbjct: 387 GGGTAKGTPMHE---TPRRA-TPLQGRSSFTK-NNEFTYTTPRKSNLAGAFFTESELGPS 446

Query: 421 PSEVAAQMATQKVDDNESSIVGGLSLNDSVEGLQSKLERWRTELPPVYDRGELSSFPPSE 480
           PSEVAA+MA QK+DDN SS V G+S+N+SVEGLQSKLERWRTELPPV+DRGE  SFP SE
Sbjct: 447 PSEVAAKMAMQKIDDNASSTVDGVSVNESVEGLQSKLERWRTELPPVFDRGESPSFPKSE 506

Query: 481 SRHNRRHSDGGGLFSCFSNICGCECSIVCGGGSQKAPKKSPSGRVGRSPSADNVSFM 537
            RHNR HSDGGGLFSCFSNICGCECSIVC GGS K PKKS   RVGRSPSADNVSF+
Sbjct: 507 PRHNRSHSDGGGLFSCFSNICGCECSIVC-GGSPKTPKKSGGSRVGRSPSADNVSFV 522

BLAST of Spg023271 vs. TAIR 10
Match: AT1G04540.1 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 354.4 bits (908), Expect = 1.6e-97
Identity = 246/598 (41.14%), Postives = 344/598 (57.53%), Query Frame = 0

Query: 2   RTYAVAWVHPDRKLSTRVDTHGHNNPTWNDKFVFRVDDEFLHADTSAVMIEIYALHWFKD 61
           +TYAVAWVH +RKL+TRVD +G  NPTWNDKFVFRV++EFL+ADTSAV+IEIYALHWF+D
Sbjct: 29  KTYAVAWVHSERKLTTRVDYNGGTNPTWNDKFVFRVNEEFLYADTSAVVIEIYALHWFRD 88

Query: 62  IHVGTVRILVGNLIPTPPR------LHQFSQQPQVGMRFVALQVRRPSGRPQGILNIGVA 121
           +HVGTVR+L+ NLIP   R       +++ + P  GMRFVALQVRR SGRPQGILNIGV 
Sbjct: 89  VHVGTVRVLISNLIPPNRRPGYRTSNNEYRRTPPPGMRFVALQVRRTSGRPQGILNIGVG 148

Query: 122 LLHSSMRSMPLYSQLSTSAIGYRNLMGEEDPFHNH-HSNTNNHSHGTP---------TVV 181
           L+  SMRSMPLY+ + +SA+GYR+L+GEED    H H N+N  S   P         +V+
Sbjct: 149 LIDGSMRSMPLYTHMDSSAVGYRDLLGEEDHHLQHLHLNSNKGSSKNPQSPSSRQYQSVI 208

Query: 182 TRPEMRRSKSDTSSMMGSELRGSEMMGKGMKSKASSMINGSE-----VSKHKKKGRSKAS 241
           +RPE+RR+KSDTSSM+ S+L       +    + +S I  SE      +    + +S   
Sbjct: 209 SRPELRRTKSDTSSMVVSDLLSRAERSRLANRQPASAIVSSESETLPTTTDSDEKKSSEY 268

Query: 242 SMISGSVVIKRKGKSRKASSMLSGSGIEEAYNKQNGKNGKSNAVIVGKKEKTEPSSILSG 301
           +  S ++ + R+  +   S +++ S +E            ++ V+V ++E+ +   ++  
Sbjct: 269 TPPSKNLRVPRQRYNSIESDLINPSPME------------NHHVVVSRRERHD---VMPY 328

Query: 302 SEVYPIELPPNKKETPVHKPNSSEETFD---SPPWKKPQEKPISRPEVTDSPRGKPGQET 361
           S        P KK T + K  S ++ +D   + P+      P+    +  +P    G  +
Sbjct: 329 SSYQQTGKTPRKK-TRIEKQRSVKD-YDRGRASPYLSKHGTPLRSNIIASTPMRSNGVGS 388

Query: 362 PNSRFD---------KMPYATPLRTPQRLAGGGGTARATPMHEKFATPRRTPTPLHGKSS 421
              R +          M  +TP+RTP R         +TPM           TP+     
Sbjct: 389 TPMRSNIIAMSPMHPNMVGSTPMRTPMR----SNMVGSTPMRSNIV----GSTPIRSNYM 448

Query: 422 HTKYNNDFNYTTPRKSFLGGAILTESELGPSPSEVAAQMATQKVDDNESSIVGGLSLND- 481
            T      ++ TP ++  G  ILTESELGPSPSEVA ++A  +  + ESSI+   S+++ 
Sbjct: 449 ATPMRTHHDFGTPVRNLAGRRILTESELGPSPSEVADKLAKDRSHETESSILSEWSIDES 508

Query: 482 SVEGLQSKLERWRTELPPVYDRG---------ELSSFP--------------PSESRHNR 537
           S+EGL+SKLERWRTELPP+YD G         + +S P              P+  +HNR
Sbjct: 509 SIEGLRSKLERWRTELPPLYDIGSSHISSTDYDGASVPAATAGGGMSSRRKTPTTKKHNR 568

BLAST of Spg023271 vs. TAIR 10
Match: AT2G33320.1 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 336.3 bits (861), Expect = 4.6e-92
Identity = 243/594 (40.91%), Postives = 331/594 (55.72%), Query Frame = 0

Query: 1   MRTYAVAWVHPDRKLSTRVDTHGHNNPTWNDKFVFRVDDEFLHADTSAVMIEIYALHWFK 60
           M+TYAVAWVH +RKL+TRVD  G  NPTWNDKFVFRV ++FL+ADTSAV++EIYALHWF+
Sbjct: 27  MKTYAVAWVHSERKLTTRVDYTGGGNPTWNDKFVFRVSEDFLYADTSAVVVEIYALHWFR 86

Query: 61  DIHVGTVRILVGNLIPTPPR-----LHQFSQQPQVGMRFVALQVRRPSGRPQGILNIGVA 120
           D+HVGTVR+L+ NLIP   R       ++ + P  GMRFVALQVRRPSGRPQGILNIGV 
Sbjct: 87  DVHVGTVRVLISNLIPPNRRPGYRSNEEYRRTPPPGMRFVALQVRRPSGRPQGILNIGVG 146

Query: 121 LLHSSMRSMPLYSQLSTSAIGYRNLMGEEDP-FHNHHSNTNNHSHGTP---------TVV 180
           +L  SMRSMPLY+ + +SA+GYR+L+GEEDP   N H N+   S   P         +VV
Sbjct: 147 ILDGSMRSMPLYTNMDSSAVGYRDLLGEEDPNLQNLHLNSFKGSSKNPQSPSSKQYQSVV 206

Query: 181 TRPEM-RRSKSDTSSMMGSELRGSEMMGKGMKSKASSMINGSEVSKHKKKGRSKASSMIS 240
           +RP M RR++SDTSSM+ S+L       +    K +S +  +E         +   S+ S
Sbjct: 207 SRPPMLRRTRSDTSSMVVSDLLSRVERSRVANRKPASALMSAE--SETVPTTTGHDSVTS 266

Query: 241 GSVVIKRKGKSRKASSMLSGSGIEEAYNKQNGKNGKSNAVIVGKKEKTEPSSILSGSEVY 300
            S + K    +    S    S   ++Y         ++ V+  + E+      +  S   
Sbjct: 267 DSELTKSIEYALPYQSPKIPSQGYDSYEPDFIDQSPNHNVLRPRSERQHEPDFIDQS--- 326

Query: 301 PIELPPNKKETPVHKPNSSEETFDSPPWKKPQEKPISRPEVTDSPRGKPGQETPNSRFDK 360
           P       ++T    P  S    + P   +  ++  SR     S  G P +         
Sbjct: 327 PFRSNDRSRKT----PRRSTPMIEKPRPPRDYDRTSSRASPYLSRHGTPLR-------SN 386

Query: 361 MPYATPLR------TPQRLAGGGGTARATPMHEKFATPRRT----PTPLHGKSSHTKYNN 420
           +  +TP+R      +P R  G G T R + +    +TP R+     TP+      T   +
Sbjct: 387 IVASTPIRSNMVSSSPMRSTGVGSTPRRSNI--LGSTPLRSNIVGSTPIRSNYKATPMKS 446

Query: 421 DFNYTTPRKSFLGG-AILTESELGPSPSEVAAQMATQKVD--DNESSIVGGLSLND--SV 480
              + TP +S L G  +LTESELGPSPSEVA +MA ++    + ESSI+   SL+D  ++
Sbjct: 447 PMQFGTPMRSNLAGRPVLTESELGPSPSEVAQKMAKERSQAYETESSILSEWSLDDDSNI 506

Query: 481 EGLQSKLERWRTELPPVYDRG-----------------------ELSSFPPSESRHNRRH 537
           EGL+SKLERWRTELPP+YD G                            P  + +HNRRH
Sbjct: 507 EGLRSKLERWRTELPPLYDLGSSHQSSDVGSGAMVVANVGGGKSSRKKTPAVKKKHNRRH 566

BLAST of Spg023271 vs. TAIR 10
Match: AT2G13350.1 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 193.7 bits (491), Expect = 3.7e-49
Identity = 183/555 (32.97%), Postives = 241/555 (43.42%), Query Frame = 0

Query: 1   MRTYAVAWVHPDRKLSTRVDTHGHNNPTWNDKFVFRVDDEFLHADTSAVMIEIYALHWFK 60
           M+TYA+AW+ P+RKL+TRVD  G  +PTWNDKFVFR+D+E L+  TS V+IEIYALHWFK
Sbjct: 27  MKTYAIAWIDPERKLTTRVDNTGGTSPTWNDKFVFRLDEEALYDATSIVVIEIYALHWFK 86

Query: 61  DIHVGTVRILVGNLIPTPPRLHQFSQQPQVGMRFVALQVRRPSGRPQGILNIGVALLHSS 120
           DIHVGTV+ L+ +L+            P   MRFV L+V R SGRP G+LNI V L+ +S
Sbjct: 87  DIHVGTVQALISDLV-----------SPSSAMRFVTLEVLRASGRPHGLLNIAVGLIDNS 146

Query: 121 MRSMPLYSQLSTSAIGYRNLMGEEDPFHNHHSNTNNHSHGTPTVVTRPEMRRSKSDTSSM 180
            +SMP              L+ EED    H  N ++   G         +RRSKSDTSSM
Sbjct: 147 GQSMP--------------LLFEEDLLF-HKKNISSKPVG---------LRRSKSDTSSM 206

Query: 181 MGSELRGSEMMGKGMKSKASSMINGSEVSKHKKKGRSKASSMISGSVVIKRKGKSRKASS 240
           + S                                              ++  +SR +S+
Sbjct: 207 VDSP---------------------------------------------RKVTQSRVSST 266

Query: 241 MLSGSGIEEAYNKQNGKNGKSNAVIVGKKEKTEPSSILSGSEVYPIELPPNKKETPVHKP 300
             SG                                                        
Sbjct: 267 TNSG-------------------------------------------------------- 326

Query: 301 NSSEETFDSPPWKKPQEKPISRPEVTDSPRGKPGQETPNSRFDKMPYATPLRTPQRLAGG 360
                 FD   +    +  +  P+          ++TPN         T  +T Q+L  G
Sbjct: 327 ------FDKDEFSSDSQMVVYEPQ----------RKTPN---------TMRQTKQQLVYG 386

Query: 361 GGTARATPMHEKFATPRRTPTPLHGKSSHTKYNNDFNYTTPRKSFLGGAILTESELGPSP 420
                 TPM      PR+T       +++T   N   Y TP +S     ++TES+LGPS 
Sbjct: 387 ------TPMR-----PRKT-------TAYTPKRNTIEYGTPMRS--RPIVITESDLGPSA 396

Query: 421 SEVAAQMATQKV---DDNESSIVGGLSLND-SVEGLQSKLERWRTELPPVYDRGE----- 480
           S VAAQ+A +K     D ES+++   S+ + SVEGL+SKLERW+  LP V D G      
Sbjct: 447 SVVAAQIAKEKALTGKDAESTVI---SVGERSVEGLRSKLERWQANLPVVLDVGSSYQPS 396

Query: 481 -----------LSSFPPSE----------------SRHNRRHSDGGGLFSCFSNICGCEC 519
                       SS+ P+E                S  N++  D  GLFSCF NICG EC
Sbjct: 507 SDYKTNSNFNPKSSYKPNEIVPRNPQMIGAPIQKPSGRNKKSGD-NGLFSCFGNICGIEC 396

BLAST of Spg023271 vs. TAIR 10
Match: AT3G04360.1 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 117.1 bits (292), Expect = 4.4e-26
Identity = 104/282 (36.88%), Postives = 142/282 (50.35%), Query Frame = 0

Query: 1   MRTYAVAWVHPD--RKLSTRVDTHGHNNPTWNDKFVFRVDDEFLHADTSAVMIEIYALHW 60
           M+TY+VAW++ D  RKL+TRVD     NP WN+KFVFRV+D+ L+ D SA++IEIYA  W
Sbjct: 28  MKTYSVAWINTDPMRKLTTRVDQSNRANPIWNEKFVFRVNDKILYVDASAIVIEIYAAAW 87

Query: 61  FKDIHVGTVRILVGNLIPTPPRLHQFSQQPQVG-----MRFVALQVRRPSGRPQGILNIG 120
            KD  VGTV +L+ +L         F      G     MR V LQ+RRPSGR QG L +G
Sbjct: 88  AKDALVGTVNVLLSDLFAP---WSGFGDGNDGGGGNNNMRLVTLQIRRPSGRLQGFLRLG 147

Query: 121 VALLHSSMRSMPLYSQL-STSAIGYRN-LMGEEDPFHNHHSNTNNHSHGTPTVVTRPEMR 180
           VALL    RSMPL  ++   S  G RN +  +++     H  TN+          + ++ 
Sbjct: 148 VALLDGGQRSMPLSIEVFDGSRKGERNSVKRDQEASRMMHRRTNS---------DQTDLT 207

Query: 181 RSKSDTSSMMG--SELRGSEMMGKGMKSKASSMINGSEVSKHKKKGRSKASSMISGSVVI 240
            S +D     G  +   G    G G+   A SM+NGS  S       S  ++ I+  +  
Sbjct: 208 TSTNDYGVKTGVVTGNGGGGGGGGGIVVGADSMVNGSLCSSDIGPSASVVAAAIAQGLYN 267

Query: 241 KRK-----GKSRKASSMLSG--SGIEEAYNKQNG-KNGKSNA 264
           ++K          ASS+L G   GIE    +    KNG   A
Sbjct: 268 RQKTAVKAANKEDASSILEGKTEGIEHRVERWRAEKNGTGGA 297

BLAST of Spg023271 vs. TAIR 10
Match: AT4G01200.1 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 109.0 bits (271), Expect = 1.2e-23
Identity = 57/120 (47.50%), Postives = 76/120 (63.33%), Query Frame = 0

Query: 1   MRTYAVAWVHPDRKLSTRVDTHGHNNPTWNDKFVFRVDDEFLHADTSAVMIEIYALHWFK 60
           ++TYA  WV    KL TR+D  G  NP WNDKFVF+V  EFL ++TS V IEIYA+ + +
Sbjct: 27  LQTYASVWVDSSSKLRTRIDRIGSENPIWNDKFVFQVSPEFLSSETSGVSIEIYAVGYLR 86

Query: 61  DIHVGTVRILVGNLIPTPPRLHQFSQQPQVGMRFVALQVRRPSGRPQGILNIGVALLHSS 120
           D  +GTVR LV N +PT        + P +    VALQ+RRPSG+  G+LNI   ++ +S
Sbjct: 87  DHLIGTVRFLVSNFLPTAA-----VKVPSL----VALQIRRPSGKFHGVLNIAAMVMDAS 137