Spg023003 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg023003
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionorigin of replication complex subunit 3
Locationscaffold12: 30981809 .. 30983505 (+)
RNA-Seq ExpressionSpg023003
SyntenySpg023003
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAACTTTCATGTAGGTATAATTTAGCACAAGAGCATGGTGATCTCGTCAATCTCCATGATTGGTTCCAATCGTTCAAGACAGTTGTTAGTCATCCTCATGCTAAAGGGAATCAAAAGGCGAAGCAATGCTCTACACCAAAGAAAAGGAAAGATAAACCTAACCTTAAAAACAAAAGCGATGCATCAATTCAGTATCCTTTTACATTCTTATGTGGTGAAATTGTTGTCTGCTAATTTGATCTGCAAAACTGGTTCGTGAATGGAGTTCTATTATGTTTGTTTCTTAATTTTGGTACTTCATGACTGCAATTTTTTCAGGTTAATGCCATAGCTCCATCTCCATTTTTGGTGTTTTCTATTAGACAACATGGGTTCCATCTCAAAACCAATTGGTAATGAGTGGAGTGACCCTTCTAACATATAAATTGTGAGGTCTTCACTTTTCTTTTCAATGTGGGATCCTCAACATGCCCCCTCAAGATGGTGCCTCTTTGGGTTCACCATTCTTGGATCGGATCCCAATTTTTTTTTTATTGGACCGAATACCCGTTTGGGTTTCATGGGCTTTGATACCATATTAGACAACATGGGTTCCATCTCAAAACCAATTGGTAATGAGTGGAGTGACCCTTCTAACATATAAATTGTGAGGTCTCCACTTTTCTTTTCAATGTGGGATCCTCAACATTTTCTTCTAGAATATTGAAAAATAGGAAAAAAATCACATGGCTGTGAGTCTGTGACACTGTTCCCTGTTTCTTGTTTTGTCCTAAAAGCATTTGCAGAAATAGTTTTAATTATATATATACTGCATTTTTTCCCTTTTTCTATGAAGCATTGATCCGCTTAATATTTCTAGGATCCATTATAAGATATCCTTGAAAATCCTTAACCCCTTTTTCAACAGAGCAAGGTTTTGCACTGCTGTTACTGAACTACAGATTACAGGGTTGCTTCGAATGCCAAGCAAAAGACGGCCTGATTATGTGCAGAGAGTGGCCTTCGGATTATAAGAACTGCAATTGGTCCAAGCTCAATGATCCATACAAAATGACTGAGAATCCTCAGCTTTATAGCTAAGGTGATTGATGCTAATGTGTACTTTTTCTTGGAAAAATCTTATTGATATTTTTATTATTTTTAAAGTGTAGAAATTGACTGAAACAGGCCATTAAAAGGCTGAGACGAACGGATTGTCGAGAATGTAGAGGAAAAATGAAGAGTTAGTCAAATCCAATATGAATCAGACGAAGATGGTAGTTAGAGTTGTCAGCTCTCTTAAAATTCTTTCGTCTTAGACATCTGGAGAAGAGGATCAAGTTATATCGGGCCCTCGCCTCAAGAGGGAATAGAGCAGTCAATTGTTTTATAGAATGATCGAAACATTTTCTTTTTCAAACATTGGTTAAAACAATTTTGATTATTGAACCATGGAATAATTTTCGATATGCTGCAAGTTTGTAATAGTTCAATTGTATTTCCACCAGTAACACTCTGAGAAGTTGATGTACTGATTTTCGATGGATGAGAGATGGGGTGATTTGTTGATTGTTTAAATAGTAAAGCCGCTCATGTCGAGCTTTATAGACCAACCAATATATTAAATTTTTGACATTTTCATCGACATTTTCAATTTTTTTTTTTATTTTTATTCACAATATGTGGGGAGGGGATTTCGAACCACAGACTTTGTG

mRNA sequence

ATGGAACTTTCATGTAGGTATAATTTAGCACAAGAGCATGGTGATCTCGTCAATCTCCATGATTGGTTCCAATCGTTCAAGACAGTTGTTAGTCATCCTCATGCTAAAGGGAATCAAAAGGCGAAGCAATGCTCTACACCAAAGAAAAGGAAAGATAAACCTAACCTTAAAAACAAAAGCGATGCATCAATTCAAGCAAGGTTTTGCACTGCTGTTACTGAACTACAGATTACAGGGTTGCTTCGAATGCCAAGCAAAAGACGGCCTGATTATGTGCAGAGAGTGGCCTTCGGATTATAA

Coding sequence (CDS)

ATGGAACTTTCATGTAGGTATAATTTAGCACAAGAGCATGGTGATCTCGTCAATCTCCATGATTGGTTCCAATCGTTCAAGACAGTTGTTAGTCATCCTCATGCTAAAGGGAATCAAAAGGCGAAGCAATGCTCTACACCAAAGAAAAGGAAAGATAAACCTAACCTTAAAAACAAAAGCGATGCATCAATTCAAGCAAGGTTTTGCACTGCTGTTACTGAACTACAGATTACAGGGTTGCTTCGAATGCCAAGCAAAAGACGGCCTGATTATGTGCAGAGAGTGGCCTTCGGATTATAA

Protein sequence

MELSCRYNLAQEHGDLVNLHDWFQSFKTVVSHPHAKGNQKAKQCSTPKKRKDKPNLKNKSDASIQARFCTAVTELQITGLLRMPSKRRPDYVQRVAFGL
Homology
BLAST of Spg023003 vs. NCBI nr
Match: XP_022159468.1 (origin of replication complex subunit 3 isoform X1 [Momordica charantia] >XP_022159469.1 origin of replication complex subunit 3 isoform X1 [Momordica charantia])

HSP 1 Score: 177.9 bits (450), Expect = 4.2e-41
Identity = 84/93 (90.32%), Postives = 89/93 (95.70%), Query Frame = 0

Query: 7   YNLAQEHGDLVNLHDWFQSFKTVVSHPHAKGNQKAKQCSTPKKRKDKPNLKNKSDASIQA 66
           YNLAQEHGDL+NLHDWFQSFKTVVSH HAK N + KQCSTPKKRKDKP+L+NKSDAS+QA
Sbjct: 648 YNLAQEHGDLINLHDWFQSFKTVVSHTHAKWNHRTKQCSTPKKRKDKPSLENKSDASVQA 707

Query: 67  RFCTAVTELQITGLLRMPSKRRPDYVQRVAFGL 100
           RFCTAVTELQITGLLRMPSKRRPDYVQRVAFGL
Sbjct: 708 RFCTAVTELQITGLLRMPSKRRPDYVQRVAFGL 740

BLAST of Spg023003 vs. NCBI nr
Match: XP_022159471.1 (origin of replication complex subunit 3 isoform X3 [Momordica charantia])

HSP 1 Score: 177.9 bits (450), Expect = 4.2e-41
Identity = 84/93 (90.32%), Postives = 89/93 (95.70%), Query Frame = 0

Query: 7   YNLAQEHGDLVNLHDWFQSFKTVVSHPHAKGNQKAKQCSTPKKRKDKPNLKNKSDASIQA 66
           YNLAQEHGDL+NLHDWFQSFKTVVSH HAK N + KQCSTPKKRKDKP+L+NKSDAS+QA
Sbjct: 645 YNLAQEHGDLINLHDWFQSFKTVVSHTHAKWNHRTKQCSTPKKRKDKPSLENKSDASVQA 704

Query: 67  RFCTAVTELQITGLLRMPSKRRPDYVQRVAFGL 100
           RFCTAVTELQITGLLRMPSKRRPDYVQRVAFGL
Sbjct: 705 RFCTAVTELQITGLLRMPSKRRPDYVQRVAFGL 737

BLAST of Spg023003 vs. NCBI nr
Match: XP_022159470.1 (origin of replication complex subunit 3 isoform X2 [Momordica charantia])

HSP 1 Score: 177.9 bits (450), Expect = 4.2e-41
Identity = 84/93 (90.32%), Postives = 89/93 (95.70%), Query Frame = 0

Query: 7   YNLAQEHGDLVNLHDWFQSFKTVVSHPHAKGNQKAKQCSTPKKRKDKPNLKNKSDASIQA 66
           YNLAQEHGDL+NLHDWFQSFKTVVSH HAK N + KQCSTPKKRKDKP+L+NKSDAS+QA
Sbjct: 647 YNLAQEHGDLINLHDWFQSFKTVVSHTHAKWNHRTKQCSTPKKRKDKPSLENKSDASVQA 706

Query: 67  RFCTAVTELQITGLLRMPSKRRPDYVQRVAFGL 100
           RFCTAVTELQITGLLRMPSKRRPDYVQRVAFGL
Sbjct: 707 RFCTAVTELQITGLLRMPSKRRPDYVQRVAFGL 739

BLAST of Spg023003 vs. NCBI nr
Match: XP_023534344.1 (origin of replication complex subunit 3 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 172.6 bits (436), Expect = 1.7e-39
Identity = 83/93 (89.25%), Postives = 87/93 (93.55%), Query Frame = 0

Query: 7   YNLAQEHGDLVNLHDWFQSFKTVVSHPHAKGNQKAKQCSTPKKRKDKPNLKNKSDASIQA 66
           YNLAQEHGDL+NLHDWFQSFKTVVSHP  KGN + KQ STPKK+KDKP L+NKSDASIQA
Sbjct: 647 YNLAQEHGDLINLHDWFQSFKTVVSHPRTKGNHRTKQGSTPKKKKDKPILENKSDASIQA 706

Query: 67  RFCTAVTELQITGLLRMPSKRRPDYVQRVAFGL 100
           RFCTAVTELQITGLLRMPSKRRPDYVQRVAFGL
Sbjct: 707 RFCTAVTELQITGLLRMPSKRRPDYVQRVAFGL 739

BLAST of Spg023003 vs. NCBI nr
Match: XP_023534346.1 (origin of replication complex subunit 3 isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 172.6 bits (436), Expect = 1.7e-39
Identity = 83/93 (89.25%), Postives = 87/93 (93.55%), Query Frame = 0

Query: 7   YNLAQEHGDLVNLHDWFQSFKTVVSHPHAKGNQKAKQCSTPKKRKDKPNLKNKSDASIQA 66
           YNLAQEHGDL+NLHDWFQSFKTVVSHP  KGN + KQ STPKK+KDKP L+NKSDASIQA
Sbjct: 645 YNLAQEHGDLINLHDWFQSFKTVVSHPRTKGNHRTKQGSTPKKKKDKPILENKSDASIQA 704

Query: 67  RFCTAVTELQITGLLRMPSKRRPDYVQRVAFGL 100
           RFCTAVTELQITGLLRMPSKRRPDYVQRVAFGL
Sbjct: 705 RFCTAVTELQITGLLRMPSKRRPDYVQRVAFGL 737

BLAST of Spg023003 vs. ExPASy Swiss-Prot
Match: Q6E7H0 (Origin of replication complex subunit 3 OS=Arabidopsis thaliana OX=3702 GN=ORC3 PE=1 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 2.0e-25
Identity = 59/98 (60.20%), Postives = 73/98 (74.49%), Query Frame = 0

Query: 2   ELSCRYNLAQEHGDLVNLHDWFQSFKTVVSHPHAKGNQKAKQCSTPKKRKD-KPNLKNKS 61
           + S  Y LAQEH D++NLHDW+QSFKT++    +K  QK+K  S  KKRK+     +  +
Sbjct: 636 DTSILYKLAQEHADVINLHDWYQSFKTILIPRSSKAKQKSKSSSKSKKRKEICEEPEAPA 695

Query: 62  DASIQARFCTAVTELQITGLLRMPSKRRPDYVQRVAFG 99
           +A IQARFC AV ELQITGL+RMPSKRRPD+VQRVAFG
Sbjct: 696 EALIQARFCRAVMELQITGLIRMPSKRRPDFVQRVAFG 733

BLAST of Spg023003 vs. ExPASy Swiss-Prot
Match: Q0IY07 (Origin of replication complex subunit 3 OS=Oryza sativa subsp. japonica OX=39947 GN=ORC3 PE=2 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 7.4e-25
Identity = 59/98 (60.20%), Postives = 75/98 (76.53%), Query Frame = 0

Query: 2   ELSCRYNLAQEHGDLVNLHDWFQSFKTVVSHPHAKGNQKAKQCSTPKKRKDKPNLKNKSD 61
           + S   NLAQE+GD++NLHDW+ SF  +++  H+K   K K  ++P K+K KP +  +S+
Sbjct: 624 DTSIMCNLAQEYGDVINLHDWYISFDGIINSVHSK--IKRKPHTSPSKKKSKP-VAAESE 683

Query: 62  ASIQARFCTAVTELQITGLLRMPSKRRPDYVQRVAFGL 100
           A IQARFC AVTELQITGLLRMPSKRRPD VQR+AFGL
Sbjct: 684 AMIQARFCRAVTELQITGLLRMPSKRRPDLVQRIAFGL 718

BLAST of Spg023003 vs. ExPASy Swiss-Prot
Match: Q10067 (Origin recognition complex subunit 3 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=orc3 PE=1 SV=2)

HSP 1 Score: 44.3 bits (103), Expect = 9.4e-04
Identity = 30/107 (28.04%), Postives = 53/107 (49.53%), Query Frame = 0

Query: 2   ELSCRYNLAQEHGDLVNLHDWFQSFKTVVSHPHAKGNQKAKQCSTPKKRKDKPNLKNK-- 61
           +LS  Y L  E G L+NL+DW+ +F     H  A G +  K+     +   + NL+ +  
Sbjct: 587 DLSILYKLYSESGALLNLYDWYIAFS---EHLQA-GKENLKEDDNHPRESPQGNLQRELN 646

Query: 62  ---------SDASIQARFCTAVTELQITGLLRMPSKRRPDYVQRVAF 98
                     +A +Q+RF   + EL+  GL++ P+ R+ D+V +  +
Sbjct: 647 ELDEDKRKLEEAKLQSRFLFGLEELRFLGLIK-PTARKTDHVMKTIY 688

BLAST of Spg023003 vs. ExPASy TrEMBL
Match: A0A6J1DYW4 (origin of replication complex subunit 3 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111025876 PE=3 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 2.0e-41
Identity = 84/93 (90.32%), Postives = 89/93 (95.70%), Query Frame = 0

Query: 7   YNLAQEHGDLVNLHDWFQSFKTVVSHPHAKGNQKAKQCSTPKKRKDKPNLKNKSDASIQA 66
           YNLAQEHGDL+NLHDWFQSFKTVVSH HAK N + KQCSTPKKRKDKP+L+NKSDAS+QA
Sbjct: 648 YNLAQEHGDLINLHDWFQSFKTVVSHTHAKWNHRTKQCSTPKKRKDKPSLENKSDASVQA 707

Query: 67  RFCTAVTELQITGLLRMPSKRRPDYVQRVAFGL 100
           RFCTAVTELQITGLLRMPSKRRPDYVQRVAFGL
Sbjct: 708 RFCTAVTELQITGLLRMPSKRRPDYVQRVAFGL 740

BLAST of Spg023003 vs. ExPASy TrEMBL
Match: A0A6J1DYU6 (origin of replication complex subunit 3 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111025876 PE=3 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 2.0e-41
Identity = 84/93 (90.32%), Postives = 89/93 (95.70%), Query Frame = 0

Query: 7   YNLAQEHGDLVNLHDWFQSFKTVVSHPHAKGNQKAKQCSTPKKRKDKPNLKNKSDASIQA 66
           YNLAQEHGDL+NLHDWFQSFKTVVSH HAK N + KQCSTPKKRKDKP+L+NKSDAS+QA
Sbjct: 647 YNLAQEHGDLINLHDWFQSFKTVVSHTHAKWNHRTKQCSTPKKRKDKPSLENKSDASVQA 706

Query: 67  RFCTAVTELQITGLLRMPSKRRPDYVQRVAFGL 100
           RFCTAVTELQITGLLRMPSKRRPDYVQRVAFGL
Sbjct: 707 RFCTAVTELQITGLLRMPSKRRPDYVQRVAFGL 739

BLAST of Spg023003 vs. ExPASy TrEMBL
Match: A0A6J1E410 (origin of replication complex subunit 3 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111025876 PE=3 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 2.0e-41
Identity = 84/93 (90.32%), Postives = 89/93 (95.70%), Query Frame = 0

Query: 7   YNLAQEHGDLVNLHDWFQSFKTVVSHPHAKGNQKAKQCSTPKKRKDKPNLKNKSDASIQA 66
           YNLAQEHGDL+NLHDWFQSFKTVVSH HAK N + KQCSTPKKRKDKP+L+NKSDAS+QA
Sbjct: 645 YNLAQEHGDLINLHDWFQSFKTVVSHTHAKWNHRTKQCSTPKKRKDKPSLENKSDASVQA 704

Query: 67  RFCTAVTELQITGLLRMPSKRRPDYVQRVAFGL 100
           RFCTAVTELQITGLLRMPSKRRPDYVQRVAFGL
Sbjct: 705 RFCTAVTELQITGLLRMPSKRRPDYVQRVAFGL 737

BLAST of Spg023003 vs. ExPASy TrEMBL
Match: A0A6J1H3F7 (origin of replication complex subunit 3 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459306 PE=3 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 1.4e-39
Identity = 83/93 (89.25%), Postives = 87/93 (93.55%), Query Frame = 0

Query: 7   YNLAQEHGDLVNLHDWFQSFKTVVSHPHAKGNQKAKQCSTPKKRKDKPNLKNKSDASIQA 66
           YNLAQEHGDL+NLHDWFQSFKTVVSHP  KGN + KQ STPKK+KDKP L+NKSDASIQA
Sbjct: 648 YNLAQEHGDLINLHDWFQSFKTVVSHPGTKGNHRTKQGSTPKKKKDKPILENKSDASIQA 707

Query: 67  RFCTAVTELQITGLLRMPSKRRPDYVQRVAFGL 100
           RFCTAVTELQITGLLRMPSKRRPDYVQRVAFGL
Sbjct: 708 RFCTAVTELQITGLLRMPSKRRPDYVQRVAFGL 740

BLAST of Spg023003 vs. ExPASy TrEMBL
Match: A0A6J1K0R6 (origin of replication complex subunit 3 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111491354 PE=3 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 1.4e-39
Identity = 83/93 (89.25%), Postives = 87/93 (93.55%), Query Frame = 0

Query: 7   YNLAQEHGDLVNLHDWFQSFKTVVSHPHAKGNQKAKQCSTPKKRKDKPNLKNKSDASIQA 66
           YNLAQEHGDL+NLHDWFQSFKTVVSHP  KGN + KQ STPKK+KDKP L+NKSDASIQA
Sbjct: 647 YNLAQEHGDLINLHDWFQSFKTVVSHPRTKGNLRTKQGSTPKKKKDKPILENKSDASIQA 706

Query: 67  RFCTAVTELQITGLLRMPSKRRPDYVQRVAFGL 100
           RFCTAVTELQITGLLRMPSKRRPDYVQRVAFGL
Sbjct: 707 RFCTAVTELQITGLLRMPSKRRPDYVQRVAFGL 739

BLAST of Spg023003 vs. TAIR 10
Match: AT5G16690.1 (origin recognition complex subunit 3 )

HSP 1 Score: 116.3 bits (290), Expect = 1.4e-26
Identity = 59/98 (60.20%), Postives = 73/98 (74.49%), Query Frame = 0

Query: 2   ELSCRYNLAQEHGDLVNLHDWFQSFKTVVSHPHAKGNQKAKQCSTPKKRKD-KPNLKNKS 61
           + S  Y LAQEH D++NLHDW+QSFKT++    +K  QK+K  S  KKRK+     +  +
Sbjct: 636 DTSILYKLAQEHADVINLHDWYQSFKTILIPRSSKAKQKSKSSSKSKKRKEICEEPEAPA 695

Query: 62  DASIQARFCTAVTELQITGLLRMPSKRRPDYVQRVAFG 99
           +A IQARFC AV ELQITGL+RMPSKRRPD+VQRVAFG
Sbjct: 696 EALIQARFCRAVMELQITGLIRMPSKRRPDFVQRVAFG 733

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022159468.14.2e-4190.32origin of replication complex subunit 3 isoform X1 [Momordica charantia] >XP_022... [more]
XP_022159471.14.2e-4190.32origin of replication complex subunit 3 isoform X3 [Momordica charantia][more]
XP_022159470.14.2e-4190.32origin of replication complex subunit 3 isoform X2 [Momordica charantia][more]
XP_023534344.11.7e-3989.25origin of replication complex subunit 3 isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_023534346.11.7e-3989.25origin of replication complex subunit 3 isoform X3 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q6E7H02.0e-2560.20Origin of replication complex subunit 3 OS=Arabidopsis thaliana OX=3702 GN=ORC3 ... [more]
Q0IY077.4e-2560.20Origin of replication complex subunit 3 OS=Oryza sativa subsp. japonica OX=39947... [more]
Q100679.4e-0428.04Origin recognition complex subunit 3 OS=Schizosaccharomyces pombe (strain 972 / ... [more]
Match NameE-valueIdentityDescription
A0A6J1DYW42.0e-4190.32origin of replication complex subunit 3 isoform X1 OS=Momordica charantia OX=367... [more]
A0A6J1DYU62.0e-4190.32origin of replication complex subunit 3 isoform X2 OS=Momordica charantia OX=367... [more]
A0A6J1E4102.0e-4190.32origin of replication complex subunit 3 isoform X3 OS=Momordica charantia OX=367... [more]
A0A6J1H3F71.4e-3989.25origin of replication complex subunit 3 isoform X1 OS=Cucurbita moschata OX=3662... [more]
A0A6J1K0R61.4e-3989.25origin of replication complex subunit 3 isoform X2 OS=Cucurbita maxima OX=3661 G... [more]
Match NameE-valueIdentityDescription
AT5G16690.11.4e-2660.20origin recognition complex subunit 3 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR040855ORC3, winged helix C-terminalPFAMPF18137ORC_WH_Ccoord: 3..97
e-value: 1.2E-18
score: 67.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 33..61
NoneNo IPR availablePANTHERPTHR12748:SF0ORIGIN RECOGNITION COMPLEX SUBUNIT 3coord: 4..98
IPR020795Origin recognition complex, subunit 3PANTHERPTHR12748ORIGIN RECOGNITION COMPLEX SUBUNIT 3coord: 4..98

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg023003.1Spg023003.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006270 DNA replication initiation
biological_process GO:0048527 lateral root development
biological_process GO:0009744 response to sucrose
biological_process GO:0006260 DNA replication
cellular_component GO:0031261 DNA replication preinitiation complex
cellular_component GO:0005664 nuclear origin of replication recognition complex
cellular_component GO:0005656 nuclear pre-replicative complex
molecular_function GO:0003688 DNA replication origin binding
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0003677 DNA binding