Homology
BLAST of Spg022862 vs. NCBI nr
Match:
XP_042955269.1 (L-ascorbate oxidase-like isoform X1 [Carya illinoinensis] >KAG2674098.1 hypothetical protein I3760_13G119700 [Carya illinoinensis] >KAG6631892.1 hypothetical protein CIPAW_13G121200 [Carya illinoinensis] >KAG6682048.1 hypothetical protein I3842_13G119400 [Carya illinoinensis])
HSP 1 Score: 550.1 bits (1416), Expect = 1.9e-152
Identity = 288/571 (50.44%), Postives = 356/571 (62.35%), Query Frame = 0
Query: 8 LVILYVLVSKIGWSQDSQIRNFQWDVEYMFWSPDCEENVVMGINDQFPGPTIKANVGDTV 67
++I ++L+ IG S ++ R+F+W+VEYMFWSPDC ENVVMGIN +FPGPTI+A GDTV
Sbjct: 21 IIIWFILLPIIGVSGGAKTRHFKWEVEYMFWSPDCVENVVMGINGEFPGPTIRAKAGDTV 80
Query: 68 VVNVTNKLFTEG------------------------------------------------ 127
V +TNKL TEG
Sbjct: 81 DVELTNKLSTEGVVIHWHGIRQFGTPWADGTASISQCVINPGETFHYRFKVDRAGTYFYH 140
Query: 128 --------------------------------RNILLS---------------------- 187
N+LLS
Sbjct: 141 GHFGMQRSAGLYGSLIVEVGEEEREPFHYDGEFNLLLSDWWHQSVHNQEVGLSSNPFRWI 200
Query: 188 -------------WTLWDAKNGRTASKYNSSSSMK--QCEFKGNEQCAPSILQVLPNKTY 247
+ A N RT +SSS+ + QC+F+GNEQCAP IL+V PNKTY
Sbjct: 201 GEPQTLLINGRGQYNCSMAANFRTPESSSSSSNTQYSQCKFRGNEQCAPHILRVNPNKTY 260
Query: 248 RLRIASTTSLAFLNVAIGVESQHDSSRADGNYVEPFTTSNINIYSGESYSVLITTNQDPT 307
RLR+ASTT+LA LN+AIG + ADGNY++PF+ +++IYSGESYSVLITT+QDP+
Sbjct: 261 RLRVASTTALASLNLAIG-NHKMVVVEADGNYLQPFSVDDLDIYSGESYSVLITTDQDPS 320
Query: 308 KNYWLSAAVTGQQPNTSSALTLLNYLPTPATDLPISPPPPTPQWNDTTRLKNFAKQISAT 367
KNYWLS V G+ P T L +LNY PT A+ LPISPPP TP+WND K F+K+I A
Sbjct: 321 KNYWLSVGVRGRLPETPPGLAILNYKPTSASKLPISPPPVTPRWNDYGHSKAFSKKIFAL 380
Query: 368 ATSPKPPQNSTRRILLLNTQNQINGAEKWALNNVSLSLPTTPYLGAIKFGLNQSFDQGTP 427
S KPP++ RRI+LLNTQN+ING KWA+NNVSL+LP TPYLG+IK+GL +FD+ +P
Sbjct: 381 MGSSKPPKSYERRIILLNTQNKINGYTKWAINNVSLALPPTPYLGSIKYGLKNAFDRNSP 440
Query: 428 PETFPENYDINIPPTNNATTVRNAIHRLDFGSTVDIVLQNANTMVKNVSEIHPWHLHGHD 462
PE F ENYD+ PPTN +T + ++ L F +TVD++LQNAN + NVSEIHPWHLHGHD
Sbjct: 441 PENFDENYDVMSPPTNPNSTSGSGVYLLSFNTTVDVILQNANALSANVSEIHPWHLHGHD 500
BLAST of Spg022862 vs. NCBI nr
Match:
KAG7950316.1 (hypothetical protein I3843_13G106200 [Carya illinoinensis])
HSP 1 Score: 549.3 bits (1414), Expect = 3.2e-152
Identity = 288/571 (50.44%), Postives = 356/571 (62.35%), Query Frame = 0
Query: 8 LVILYVLVSKIGWSQDSQIRNFQWDVEYMFWSPDCEENVVMGINDQFPGPTIKANVGDTV 67
++I ++L+ IG S ++ R+F+W+VEYMFWSPDC ENVVMGIN +FPGPTI+A GDTV
Sbjct: 21 IIIWFILLPIIGVSGVAKTRHFKWEVEYMFWSPDCVENVVMGINGEFPGPTIRAKAGDTV 80
Query: 68 VVNVTNKLFTEG------------------------------------------------ 127
V +TNKL TEG
Sbjct: 81 DVELTNKLSTEGVVIHWHGIRQFGTPWADGTASISQCVINPGETFHYRFKVDRAGTYFYH 140
Query: 128 --------------------------------RNILLS---------------------- 187
N+LLS
Sbjct: 141 GHFGMQRSAGLYGSLIVEVGEEEREPFHYDGEFNLLLSDWWHQSVHNQEVGLSSNPFRWI 200
Query: 188 -------------WTLWDAKNGRTASKYNSSSSMK--QCEFKGNEQCAPSILQVLPNKTY 247
+ A N RT +SSS+ + QC+F+GNEQCAP IL+V PNKTY
Sbjct: 201 GEPQTLLINGRGQYNCSMAANFRTPESSSSSSNTQYSQCKFRGNEQCAPHILRVNPNKTY 260
Query: 248 RLRIASTTSLAFLNVAIGVESQHDSSRADGNYVEPFTTSNINIYSGESYSVLITTNQDPT 307
RLR+ASTT+LA LN+AIG + ADGNY++PF+ +++IYSGESYSVLITT+QDP+
Sbjct: 261 RLRVASTTALASLNLAIG-NHKMVVVEADGNYLQPFSVDDLDIYSGESYSVLITTDQDPS 320
Query: 308 KNYWLSAAVTGQQPNTSSALTLLNYLPTPATDLPISPPPPTPQWNDTTRLKNFAKQISAT 367
KNYWLS V G+ P T L +LNY PT A+ LPISPPP TP+WND K F+K+I A
Sbjct: 321 KNYWLSVGVRGRLPETPPGLAILNYKPTSASKLPISPPPVTPRWNDYGHSKAFSKKIFAL 380
Query: 368 ATSPKPPQNSTRRILLLNTQNQINGAEKWALNNVSLSLPTTPYLGAIKFGLNQSFDQGTP 427
S KPP++ RRI+LLNTQN+ING KWA+NNVSL+LP TPYLG+IK+GL +FD+ +P
Sbjct: 381 MGSSKPPKSYERRIILLNTQNKINGYTKWAINNVSLALPPTPYLGSIKYGLKNAFDRNSP 440
Query: 428 PETFPENYDINIPPTNNATTVRNAIHRLDFGSTVDIVLQNANTMVKNVSEIHPWHLHGHD 462
PE F ENYD+ PPTN +T + ++ L F +TVD++LQNAN + NVSEIHPWHLHGHD
Sbjct: 441 PENFDENYDVMSPPTNPNSTSGSGVYLLSFNTTVDVILQNANALSANVSEIHPWHLHGHD 500
BLAST of Spg022862 vs. NCBI nr
Match:
XP_038880052.1 (L-ascorbate oxidase-like [Benincasa hispida])
HSP 1 Score: 546.6 bits (1407), Expect = 2.1e-151
Identity = 295/570 (51.75%), Postives = 352/570 (61.75%), Query Frame = 0
Query: 6 VLLVILYVLVSKIGWSQDSQIRN---FQWDVEYMFWSPDCEENVVMGINDQFPGPTIKAN 65
+++ +++ ++S G + + N F+W+VEYMFWSPDC EN+VMGIN QFPGPTI+AN
Sbjct: 15 LVIFLIFSMISGFGITLSEAVNNTRYFKWEVEYMFWSPDCVENLVMGINGQFPGPTIRAN 74
Query: 66 VGDTVVVNVTNKLFTEG------------------------------------------- 125
VGDT++V + NKLFTEG
Sbjct: 75 VGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAINPGETFIYQFKADKPG 134
Query: 126 -------------------------------------RNILLS--W-------------- 185
N+LLS W
Sbjct: 135 TYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDWWHKSAHKQEVGLSSS 194
Query: 186 -TLWDAK------NGR------TASKYNSSSSMKQCEFKGNEQCAPSILQVLPNKTYRLR 245
W + NGR A+KYN +++QC+ KG EQCAP ILQV P KTYR+R
Sbjct: 195 PMRWIGEPETILINGRGQISCSIAAKYN--KNLEQCKLKGGEQCAPFILQVKPKKTYRIR 254
Query: 246 IASTTSLAFLNVAIGVESQHDSSRADGNYVEPFTTSNINIYSGESYSVLITTNQDPTKNY 305
IASTTSL+ LN+AIG Q ADGNYVEPF TS+I+IYSGESYSVLITTNQDP +NY
Sbjct: 255 IASTTSLSALNLAIG-NHQLLVVEADGNYVEPFVTSDIDIYSGESYSVLITTNQDPAQNY 314
Query: 306 WLSAAVTGQQPNTSSALTLLNYLPTPATDLPISPPPPTPQWNDTTRLKNFAKQISATATS 365
W+S V G+ P T ALTLLNYLP LP+SPPP TP W D R KNF +I A S
Sbjct: 315 WVSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSKNFTYRILAAMGS 374
Query: 366 PKPPQNSTRRILLLNTQNQINGA-EKWALNNVSLSLPTTPYLGAIKFGLNQSFDQGTPPE 425
P PP RRI LLNTQN++NG KWA+NNVSLSLP+TPYLGAIK GLN +F+Q PPE
Sbjct: 375 PNPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIGLNNTFNQNPPPE 434
Query: 426 TFPENYDINIPPTNNATTVRNAIHRLDFGSTVDIVLQNANTMVKNVSEIHPWHLHGHDFW 462
TF E+YDI PP N T N ++ + G TVD++LQNANT+ SEIHPWHLHGHDFW
Sbjct: 435 TFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSEIHPWHLHGHDFW 494
BLAST of Spg022862 vs. NCBI nr
Match:
KAF7831515.1 (L-ascorbate oxidase-like [Senna tora])
HSP 1 Score: 545.8 bits (1405), Expect = 3.5e-151
Identity = 271/498 (54.42%), Postives = 348/498 (69.88%), Query Frame = 0
Query: 6 VLLVILYVLVSKIGWSQDSQIRNFQWDVEYMFWSPDCEENVVMGINDQFPGPTIKANVGD 65
V +I++V + ++ W +++R+F+W+VEYM+ +PDC E VV+GIN QFPGPT++A VGD
Sbjct: 12 VSFMIIWVCLCQVSWLLGAKVRHFEWEVEYMYGAPDCLEGVVIGINGQFPGPTVRAQVGD 71
Query: 66 TVVVNVTNKLFTEGRNILLSW-------TLWD---------------------------A 125
TV V +TNKL TEG +++ W T W
Sbjct: 72 TVSVALTNKLSTEG--VVIHWHGIRQRGTPWADGTASISQCAINPGETFHYRFLVERSLL 131
Query: 126 KNGRTASKYNSS-------SSMKQCEFKGNEQCAPSILQVLPNKTYRLRIASTTSLAFLN 185
NGR ++N S +++ QC F+GNEQCAP IL VLPN TYR+RIAS+T+LA LN
Sbjct: 132 MNGR--GQFNCSMASGLINTTLAQCNFRGNEQCAPQILHVLPNNTYRIRIASSTALASLN 191
Query: 186 VAIGVESQHDSSRADGNYVEPFTTSNINIYSGESYSVLITTNQDPTKNYWLSAAVTGQQP 245
AIG + ADGNYV+PFT +I+IYSGESYSVL+TTNQDPT+NYW++ V G++P
Sbjct: 192 FAIG-NHKMVVVEADGNYVQPFTVEDIDIYSGESYSVLLTTNQDPTQNYWIAIGVRGRKP 251
Query: 246 NTSSALTLLNYLPTPATDLPISPPPPTPQWNDTTRLKNFAKQISATATSPKPPQNSTRRI 305
T ALT+LNY PT A+ LP PPP TP WN+ T K+F++++ A SPKPP+ + RR+
Sbjct: 252 KTPQALTILNYKPTWASKLPPVPPPITPLWNNYTHSKSFSQKVLALVGSPKPPKKAQRRV 311
Query: 306 LLLNTQNQINGAEKWALNNVSLSLPTTPYLGAIKFGLNQSFDQGTPPETFPENYDINIPP 365
LLLNTQN+ING KWA+NNVSLSLPTTPYLG++K+ L +FD +PPE+FP YDI PP
Sbjct: 312 LLLNTQNKINGFTKWAINNVSLSLPTTPYLGSLKYNLRNAFDFKSPPESFPIEYDIMKPP 371
Query: 366 TNNATTVRNAIHRLDFGSTVDIVLQNANTMVKNVSEIHPWHLHGHDFWVLGFGEGKF-SA 425
N +T+ N ++ VD++LQNAN + +NVSEIHPWHLHGHDFWVLG+GEGKF
Sbjct: 372 MNPNSTIGNGVYVFKMKQVVDVILQNANALDENVSEIHPWHLHGHDFWVLGYGEGKFRGR 431
Query: 426 ADEKQLNLKNPALKNTVVVFPYGWIVVRFVADNPGVWIFHCHIEPHLHMGMGVVLAEGVD 462
DE++ NLKNP +KNT V+FPYGW +RF ADNPGVW FHCHIEPHLHMGMGVV AEGV
Sbjct: 432 EDEQKFNLKNPPIKNTAVIFPYGWTALRFRADNPGVWAFHCHIEPHLHMGMGVVFAEGVR 491
BLAST of Spg022862 vs. NCBI nr
Match:
XP_031283448.1 (L-ascorbate oxidase-like [Pistacia vera])
HSP 1 Score: 544.3 bits (1401), Expect = 1.0e-150
Identity = 283/565 (50.09%), Postives = 360/565 (63.72%), Query Frame = 0
Query: 4 FRVLLVILYVLVSKIGWSQDSQIRNFQWDVEYMFWSPDCEENVVMGINDQFPGPTIKANV 63
FR+++V +L ++I + S+ R+F+W+VEYMF +PDC E+VVMGIN QFPGPTI+A
Sbjct: 2 FRLVVVWCILLCTQIVIAFGSKTRHFKWEVEYMFGAPDCMEHVVMGINGQFPGPTIRAKA 61
Query: 64 GDTVVVNVTNKLFTEG-------------------------------------------- 123
GDT+ + +TNKL TEG
Sbjct: 62 GDTIAIQLTNKLHTEGVVIHWHGIRQLGTPWADGTASISQCAINPGETFLYRFKVDKAGT 121
Query: 124 ------------------------------------RNILLS--W--TLWDAKNGRTA-- 183
N+LLS W ++ D + G ++
Sbjct: 122 YFYHGHFGMQRSAGLYGSLIVDVGDGEKEPFHYDGEFNLLLSDWWHQSVHDQEVGLSSKP 181
Query: 184 ---------------SKYN-------SSSSMKQCEFKGNEQCAPSILQVLPNKTYRLRIA 243
+YN S+SS+KQC+F+GNEQCAP IL+VLPNKTYRLRIA
Sbjct: 182 FRWIGEPQTLLINGRGQYNCSMAAHFSNSSIKQCKFRGNEQCAPQILRVLPNKTYRLRIA 241
Query: 244 STTSLAFLNVAIGVESQHDSSRADGNYVEPFTTSNINIYSGESYSVLITTNQDPTKNYWL 303
STT+LA LN+AIG + ADGNYV+PF +++IYSGESYSVL TTNQD +KNYW+
Sbjct: 242 STTALASLNLAIG-NHKMVVVEADGNYVQPFDVDDMDIYSGESYSVLFTTNQDSSKNYWI 301
Query: 304 SAAVTGQQPNTSSALTLLNYLPTPATDLPISPPPPTPQWNDTTRLKNFAKQISATATSPK 363
S V G++P T AL++LNY PT A+ LP+SPPP TP+WND K+F+ +I A SPK
Sbjct: 302 SVGVRGREPKTPQALSILNYHPTSASKLPLSPPPITPKWNDYNHSKSFSNKIFALMGSPK 361
Query: 364 PPQNSTRRILLLNTQNQINGAEKWALNNVSLSLPTTPYLGAIKFGLNQSFDQGTPPETFP 423
PP+ RRI+LLNTQN ING KWA+NN+SL+LP TPY+G+IKF L +FDQ +PPE F
Sbjct: 362 PPETYQRRIILLNTQNTINGFTKWAINNISLTLPATPYIGSIKFSLKTAFDQKSPPENFS 421
Query: 424 ENYDINIPPTNNATTVRNAIHRLDFGSTVDIVLQNANTMVKNVSEIHPWHLHGHDFWVLG 461
E+YD+ PP N +T N ++ L F +TVD++LQNAN + NVSEIHPWHLHGHDFW+LG
Sbjct: 422 EDYDVMKPPVNPNSTTGNGVYMLGFNTTVDVILQNANALSANVSEIHPWHLHGHDFWMLG 481
BLAST of Spg022862 vs. ExPASy Swiss-Prot
Match:
P24792 (L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2)
HSP 1 Score: 543.1 bits (1398), Expect = 3.0e-153
Identity = 288/571 (50.44%), Postives = 353/571 (61.82%), Query Frame = 0
Query: 2 RDFRVLLVILYVLVSKIGWSQD-SQIRNFQWDVEYMFWSPDCEENVVMGINDQFPGPTIK 61
R+ L++ + L+ G S + SQIR+++W+VEYMFW+PDC EN+VMGIN QFPGPTI+
Sbjct: 8 REPNFLILFFFGLILAFGISSEGSQIRHYKWEVEYMFWAPDCNENIVMGINGQFPGPTIR 67
Query: 62 ANVGDTVVVNVTNKLFTEG----------------------------------------- 121
AN GDTVVV + NKL TEG
Sbjct: 68 ANAGDTVVVELINKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFFYNFTVDN 127
Query: 122 ---------------------------------------RNILLS--W------------ 181
N+LLS W
Sbjct: 128 PGTFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLS 187
Query: 182 ---TLWDAK------NGR------TASKYNSSSSMKQCEFKGNEQCAPSILQVLPNKTYR 241
W + NGR A+KY+ S+++ C+ KG+E CAP I V+P KTYR
Sbjct: 188 SKPIRWIGEPQTILLNGRGQFDCSIAAKYD--SNLEPCKLKGSEPCAPYIFHVMPKKTYR 247
Query: 242 LRIASTTSLAFLNVAIGVESQHD--SSRADGNYVEPFTTSNINIYSGESYSVLITTNQDP 301
+RIASTT+LA LN AIG H ADGNYV+PF TS+I+IYSGESYSVLITT+Q+P
Sbjct: 248 IRIASTTALAALNFAIG---NHPLLVVEADGNYVQPFYTSDIDIYSGESYSVLITTDQNP 307
Query: 302 TKNYWLSAAVTGQQPNTSSALTLLNYLPTPATDLPISPPPPTPQWNDTTRLKNFAKQISA 361
++NYW+S G+ PNT LTLLNYLP + LP SPPP TP W+D R KNF +I+A
Sbjct: 308 SENYWVSVGTRGRHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNFTYRITA 367
Query: 362 TATSPKPPQNSTRRILLLNTQNQINGAEKWALNNVSLSLPTTPYLGAIKFGLNQSFDQGT 421
SPKPP S RRI LLNTQN ING KWA+N+VSL+LP TPYLGA+KF L +FDQ
Sbjct: 368 AMGSPKPPVKSNRRIFLLNTQNVINGYVKWAINDVSLALPPTPYLGAMKFNLLHAFDQNP 427
Query: 422 PPETFPENYDINIPPTNNATTVRNAIHRLDFGSTVDIVLQNANTMVKNVSEIHPWHLHGH 461
PPE FPE+YDI+ PPTN T + N +++ G VD++LQNAN M +N+SEIHPWHLHGH
Sbjct: 428 PPEVFPEDYDIDTPPTNEKTKIGNGVYQFKIGEIVDVILQNANMMKENLSEIHPWHLHGH 487
BLAST of Spg022862 vs. ExPASy Swiss-Prot
Match:
P14133 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1)
HSP 1 Score: 533.9 bits (1374), Expect = 1.8e-150
Identity = 285/546 (52.20%), Postives = 332/546 (60.81%), Query Frame = 0
Query: 25 QIRNFQWDVEYMFWSPDCEENVVMGINDQFPGPTIKANVGDTVVVNVTNKLFTEG----- 84
+I++++WDVEYMFWSPDC EN+VMGIN +FPGPTI+AN GD VVV +TNKL TEG
Sbjct: 37 KIKHYKWDVEYMFWSPDCVENIVMGINGEFPGPTIRANAGDIVVVELTNKLHTEGVVIHW 96
Query: 85 RNILLSWTLW-------------------------------------------------- 144
IL T W
Sbjct: 97 HGILQRGTPWADGTASISQCAINPGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIV 156
Query: 145 DAKNGRT----------------------------------------------------- 204
D GR+
Sbjct: 157 DPPEGRSEPFHYDEEINLLLSDWWHQSVHKQEVGLSSKPMRWIGEPQSILINGKGQFDCS 216
Query: 205 -ASKYNSSSSMKQCEFKGNEQCAPSILQVLPNKTYRLRIASTTSLAFLNVAIGVESQHD- 264
A+KYN +KQCE G E+CAP IL V P KTYR+RIASTT+LA LN AIG H+
Sbjct: 217 IAAKYN--QGLKQCELSGKEKCAPFILHVQPKKTYRIRIASTTALASLNFAIG---NHEL 276
Query: 265 -SSRADGNYVEPFTTSNINIYSGESYSVLITTNQDPTKNYWLSAAVTGQQPNTSSALTLL 324
ADGNYV+PF TS+I+IYSGESYSVLITT+Q+P +NYW+S V + P T LTLL
Sbjct: 277 LVVEADGNYVQPFVTSDIDIYSGESYSVLITTDQNPLENYWVSIGVRARLPKTPPGLTLL 336
Query: 325 NYLPTPATDLPISPPPPTPQWNDTTRLKNFAKQISATATSPKPPQNSTRRILLLNTQNQI 384
NYLP A+ LPISPPP TP W D R KNF +I A SPKPP RR+ LLNTQN+I
Sbjct: 337 NYLPNSASKLPISPPPETPHWEDFDRSKNFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRI 396
Query: 385 NGAEKWALNNVSLSLPTTPYLGAIKFGLNQSFDQGTPPETFPENYDINIPPTNNATTVRN 444
NG KWA+NNVSL+LP TPYL A+K LN +F+Q PPETFP NYDIN PP N TT N
Sbjct: 397 NGFMKWAINNVSLALPPTPYLAAMKMRLNTAFNQNPPPETFPLNYDINNPPPNPETTTGN 456
Query: 445 AIHRLDFGSTVDIVLQNANTMVKNVSEIHPWHLHGHDFWVLGFGEGKFSA-ADEKQLNLK 459
+++ + G TVD++LQNAN + N+SEIHPWHLHGHDFWVLG+GEGKF A DEK+LNLK
Sbjct: 457 GVYKFNMGETVDVILQNANMLNPNMSEIHPWHLHGHDFWVLGYGEGKFYAPEDEKKLNLK 516
BLAST of Spg022862 vs. ExPASy Swiss-Prot
Match:
P37064 (L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1)
HSP 1 Score: 533.1 bits (1372), Expect = 3.1e-150
Identity = 279/546 (51.10%), Postives = 339/546 (62.09%), Query Frame = 0
Query: 24 SQIRNFQWDVEYMFWSPDCEENVVMGINDQFPGPTIKANVGDTVVVNVTNKLFTEG---- 83
SQIR+++W+VEYMFW+P+C EN+VMGIN QFPGPTI+AN GD+VVV +TNKL TEG
Sbjct: 1 SQIRHYKWEVEYMFWAPNCNENIVMGINGQFPGPTIRANAGDSVVVELTNKLHTEGVVIH 60
Query: 84 ------------------------------------------------------------ 143
Sbjct: 61 WHGILQRGTPWADGTASISQCAINPGETFFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLI 120
Query: 144 ----------------RNILLS--W---------------TLWDAK------NGR----- 203
N+LLS W W + NGR
Sbjct: 121 VDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSSKPIRWIGEPQTILLNGRGQFDC 180
Query: 204 -TASKYNSSSSMKQCEFKGNEQCAPSILQVLPNKTYRLRIASTTSLAFLNVAIGVESQHD 263
A+KY+ S+++ C+ KG+E CAP I V P KTYR+RIASTT+LA LN AIG Q
Sbjct: 181 SIAAKYD--SNLEPCKLKGSESCAPYIFHVSPKKTYRIRIASTTALAALNFAIG-NHQLL 240
Query: 264 SSRADGNYVEPFTTSNINIYSGESYSVLITTNQDPTKNYWLSAAVTGQQPNTSSALTLLN 323
ADGNYV+PF TS+I+IYSGESYSVLITT+Q+P++NYW+S + PNT LTLLN
Sbjct: 241 VVEADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVSVGTRARHPNTPPGLTLLN 300
Query: 324 YLPTPATDLPISPPPPTPQWNDTTRLKNFAKQISATATSPKPPQNSTRRILLLNTQNQIN 383
YLP + LP SPPP TP W+D R KNF +I+A SPKPP RRI LLNTQN IN
Sbjct: 301 YLPNSVSKLPTSPPPQTPAWDDFDRSKNFTYRITAAMGSPKPPVKFNRRIFLLNTQNVIN 360
Query: 384 GAEKWALNNVSLSLPTTPYLGAIKFGLNQSFDQGTPPETFPENYDINIPPTNNATTVRNA 443
G KWA+N+VSL+LP TPYLGA+K+ L +FDQ PPE FPE+YDI+ PPTN T + N
Sbjct: 361 GYVKWAINDVSLALPPTPYLGAMKYNLLHAFDQNPPPEVFPEDYDIDTPPTNEKTRIGNG 420
Query: 444 IHRLDFGSTVDIVLQNANTMVKNVSEIHPWHLHGHDFWVLGFGEGKFSAADEKQLNLKNP 461
+++ G VD++LQNAN M +N+SE HPWHLHGHDFWVLG+G+GKFSA +E LNLKNP
Sbjct: 421 VYQFKIGEVVDVILQNANMMKENLSETHPWHLHGHDFWVLGYGDGKFSAEEESSLNLKNP 480
BLAST of Spg022862 vs. ExPASy Swiss-Prot
Match:
Q40588 (L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1)
HSP 1 Score: 492.7 bits (1267), Expect = 4.7e-138
Identity = 268/568 (47.18%), Postives = 337/568 (59.33%), Query Frame = 0
Query: 4 FRVLLVILYVLVSKIGWSQDSQIRNFQWDVEYMFWSPDCEENVVMGINDQFPGPTIKANV 63
F LL ++ + +S ++ R+F+WDVEY+ WSPD EE+VVMGIN QFPGPTI+A
Sbjct: 8 FFFLLPLILLELSSSRSVMAAKTRHFKWDVEYIHWSPDGEESVVMGINGQFPGPTIRAKA 67
Query: 64 GDTVVVNVTNKLFTEG-------------------------------------------- 123
GDTV V++TNKL TEG
Sbjct: 68 GDTVAVHLTNKLHTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETFLYRFKVDKAGT 127
Query: 124 ------------------------------------RNILLS--W--------------- 183
N+LLS W
Sbjct: 128 YFYHGHYGMQRSAGLYGSLIVEVGEGEKEPFHYDGEFNLLLSDWWHKGSHEQEVDLSSNP 187
Query: 184 TLWDAK------NGRTASKYN-------SSSSMKQCEFKGNEQCAPSILQVLPNKTYRLR 243
W + NGR +YN S + QC+ +G EQ AP IL+V PNK YRLR
Sbjct: 188 LRWIGEPQTLLLNGR--GQYNCSLAARFSKPPLPQCKLRGGEQYAPQILRVRPNKIYRLR 247
Query: 244 IASTTSLAFLNVAIGVESQHDSSRADGNYVEPFTTSNINIYSGESYSVLITTNQDPTKNY 303
+ASTT+L L++AIG ADGNYV+PF+ +++IYSGESYSVL T+QDPTKNY
Sbjct: 248 VASTTALGSLSLAIGGHKM-VVVEADGNYVQPFSVQDMDIYSGESYSVLFKTDQDPTKNY 307
Query: 304 WLSAAVTGQQPNTSSALTLLNYLPTPATDLPISPPPPTPQWNDTTRLKNFAKQISATATS 363
W+S V G++P T LTLLNYLP A+ P PPP P WND K+F+ +I A S
Sbjct: 308 WISINVRGREPKTPQGLTLLNYLPNSASKFPTLPPPIAPLWNDYNHSKSFSNKIFALMGS 367
Query: 364 PKPPQNSTRRILLLNTQNQINGAEKWALNNVSLSLPTTPYLGAIKFGLNQSFDQGTPPET 423
PKPP + RRI+LLNTQN+I+G KWA+NNVSL LPT YLG+I++G+N +FD PP+
Sbjct: 368 PKPPPQNHRRIILLNTQNKIDGYTKWAINNVSLVLPTQLYLGSIRYGIN-AFDTKPPPDN 427
Query: 424 FPENYDINIPPTNNATTVRNAIHRLDFGSTVDIVLQNANTMVKNVSEIHPWHLHGHDFWV 462
FP++YD+ N+ +T N ++ L F +T+DI+LQNAN + K+VSEIHPWHLHGHDFWV
Sbjct: 428 FPKDYDVLKQAPNSNSTYGNGVYMLKFNTTIDIILQNANALAKDVSEIHPWHLHGHDFWV 487
BLAST of Spg022862 vs. ExPASy Swiss-Prot
Match:
M4DUF2 (L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1)
HSP 1 Score: 479.6 bits (1233), Expect = 4.1e-134
Identity = 254/557 (45.60%), Postives = 326/557 (58.53%), Query Frame = 0
Query: 13 VLVSKIGWSQDSQIRNFQWDVEYMFWSPDCEENVVMGINDQFPGPTIKANVGDTVVVNVT 72
V V+ + + + +R + W+VEY F PDC+E +VM +N QFPGPTI A GDT+VV++T
Sbjct: 7 VAVAILAHTASAAVREYAWEVEYKFGWPDCKEGMVMAVNGQFPGPTIHALAGDTIVVHLT 66
Query: 73 NKLFTEGR---------------------------------------------------- 132
NKL TEG
Sbjct: 67 NKLATEGLVIHWHGIRQLGSPWADGAAGVTQCAISPGETFTYNFTVDKPGTHFYHGHYGM 126
Query: 133 ----------------------------NILLS-WTLWDAK------------------- 192
N+LLS W D
Sbjct: 127 QRSAGLYGSLIIDVAKGKKEPLRYDGEFNLLLSDWWHEDVLSQEIGLSSRPMRWIGEAQS 186
Query: 193 ---NGR-----TASKYNSSSSMKQCEFKGNEQCAPSILQVLPNKTYRLRIASTTSLAFLN 252
NGR + + SS+S+ C FK +QCAP L V PNKTYR+R+AS+T+LA LN
Sbjct: 187 ILINGRGQFNCSLAAQFSSTSLPTCTFKEGDQCAPQRLHVEPNKTYRIRLASSTALASLN 246
Query: 253 VAIGVESQHDSSRADGNYVEPFTTSNINIYSGESYSVLITTNQDPTKNYWLSAAVTGQQP 312
A+ + ADGNY+ PFTT +I+IYSGE+YSVL+TT+QDP++NY+++A V G++P
Sbjct: 247 FAV-QGHKLVVVEADGNYITPFTTDDIDIYSGETYSVLLTTDQDPSQNYYITAGVRGRKP 306
Query: 313 NTSSALTLLNYLPTPATDLPISPPPPTPQWNDTTRLKNFAKQISATATSPKPPQNSTRRI 372
NT ALT+LNY+ P++ LP SPPP TP+WND R KNF+K+I A SP PP+ R+
Sbjct: 307 NTPPALTVLNYVTAPSSQLPTSPPPETPRWNDFDRSKNFSKKIFAAMGSPSPPETFDERL 366
Query: 373 LLLNTQNQINGAEKWALNNVSLSLPTTPYLGAIKFGLNQSFDQGTPPETFPENYDINIPP 432
+LLNTQN I G KWA+NNVSL++P TPYLG++K+ L F++ +PP+ +P +YDI PP
Sbjct: 367 ILLNTQNLIEGFTKWAINNVSLAVPGTPYLGSVKYNLRTGFNRSSPPKDYPVDYDIMTPP 426
Query: 433 TNNATTVRNAIHRLDFGSTVDIVLQNANTMVKNVSEIHPWHLHGHDFWVLGFGEGKFS-A 461
N N F TVD++LQNAN + N SEIHPWHLHGHDFWVLG+GEGKF
Sbjct: 427 RNRNAKQGNVSCVFPFNVTVDVILQNANGLNANASEIHPWHLHGHDFWVLGYGEGKFKPG 486
BLAST of Spg022862 vs. ExPASy TrEMBL
Match:
A0A6J1HPZ9 (L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=LOC111466657 PE=3 SV=1)
HSP 1 Score: 543.1 bits (1398), Expect = 1.1e-150
Identity = 288/571 (50.44%), Postives = 353/571 (61.82%), Query Frame = 0
Query: 2 RDFRVLLVILYVLVSKIGWSQD-SQIRNFQWDVEYMFWSPDCEENVVMGINDQFPGPTIK 61
R+ L++ + L+ G S + SQIR+++W+VEYMFW+PDC EN+VMGIN QFPGPTI+
Sbjct: 8 REPNFLILFFFGLILAFGISSEGSQIRHYKWEVEYMFWAPDCNENIVMGINGQFPGPTIR 67
Query: 62 ANVGDTVVVNVTNKLFTEG----------------------------------------- 121
AN GDTVVV + NKL TEG
Sbjct: 68 ANAGDTVVVELINKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFFYNFTVDN 127
Query: 122 ---------------------------------------RNILLS--W------------ 181
N+LLS W
Sbjct: 128 PGTFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLS 187
Query: 182 ---TLWDAK------NGR------TASKYNSSSSMKQCEFKGNEQCAPSILQVLPNKTYR 241
W + NGR A+KY+ S+++ C+ KG+E CAP I V+P KTYR
Sbjct: 188 SKPIRWIGEPQTILLNGRGQFDCSIAAKYD--SNLEPCKLKGSEPCAPYIFHVMPKKTYR 247
Query: 242 LRIASTTSLAFLNVAIGVESQHD--SSRADGNYVEPFTTSNINIYSGESYSVLITTNQDP 301
+RIASTT+LA LN AIG H ADGNYV+PF TS+I+IYSGESYSVLITT+Q+P
Sbjct: 248 IRIASTTALAALNFAIG---NHPLLVVEADGNYVQPFYTSDIDIYSGESYSVLITTDQNP 307
Query: 302 TKNYWLSAAVTGQQPNTSSALTLLNYLPTPATDLPISPPPPTPQWNDTTRLKNFAKQISA 361
++NYW+S G+ PNT LTLLNYLP + LP SPPP TP W+D R KNF +I+A
Sbjct: 308 SENYWVSVGTRGRHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNFTYRITA 367
Query: 362 TATSPKPPQNSTRRILLLNTQNQINGAEKWALNNVSLSLPTTPYLGAIKFGLNQSFDQGT 421
SPKPP S RRI LLNTQN ING KWA+N+VSL+LP TPYLGA+KF L +FDQ
Sbjct: 368 AMGSPKPPVKSNRRIFLLNTQNVINGYVKWAINDVSLALPPTPYLGAMKFNLLHAFDQNP 427
Query: 422 PPETFPENYDINIPPTNNATTVRNAIHRLDFGSTVDIVLQNANTMVKNVSEIHPWHLHGH 461
PPE FPE+YDI+ PPTN T + N +++ G VD++LQNAN M +N+SEIHPWHLHGH
Sbjct: 428 PPEVFPEDYDIDTPPTNEKTKIGNGVYQFKIGEIVDVILQNANMMKENLSEIHPWHLHGH 487
BLAST of Spg022862 vs. ExPASy TrEMBL
Match:
A0A660KQX3 (Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_010271 PE=3 SV=1)
HSP 1 Score: 542.7 bits (1397), Expect = 1.5e-150
Identity = 283/554 (51.08%), Postives = 348/554 (62.82%), Query Frame = 0
Query: 8 LVILYVLVSKIGWSQDSQIRNFQWDVEYMFWSPDCEENVVMGINDQFPGPTIKANVGDTV 67
L+I +L+ S ++ R+F+W+VEY+FWSPDC ENVVMGIN QFPGPTI+A GDT+
Sbjct: 12 LIIWSILLYFSRPSLGAKSRHFKWEVEYLFWSPDCVENVVMGINGQFPGPTIRAKAGDTI 71
Query: 68 VVNVTNKLFTEG------------------------------------------------ 127
VV +TNK TEG
Sbjct: 72 VVELTNKFSTEGFGTPWADGTASISQCAINPGETFHYRFKVDRAGTYFYHGHLGMQRSAG 131
Query: 128 ----------------------RNILLS--W---------------TLWDAK------NG 187
++LLS W W + NG
Sbjct: 132 LYGSLVVDVAEGEKEPFHYDGEFDLLLSDWWHKSVHEQEVGLSSNPFRWIGEPQSLLMNG 191
Query: 188 RTASKYN-------SSSSMKQCEFKGNEQCAPSILQVLPNKTYRLRIASTTSLAFLNVAI 247
R +YN S+SS QC+ +G EQCAP IL VLPNKTYR+R+ASTT+LA LN+AI
Sbjct: 192 R--GQYNCSLAAQFSNSSATQCKLRGTEQCAPEILHVLPNKTYRIRVASTTALASLNLAI 251
Query: 248 GVESQHDSSRADGNYVEPFTTSNINIYSGESYSVLITTNQDPTKNYWLSAAVTGQQPNTS 307
G + ADGNY++PF +++IYSGESYSVLITTNQDP+KNYW+S V G+ P T
Sbjct: 252 G-NHKMVVVEADGNYLQPFAVDDLDIYSGESYSVLITTNQDPSKNYWISVGVRGRSPATP 311
Query: 308 SALTLLNYLPTPATDLPISPPPPTPQWNDTTRLKNFAKQISATATSPKPPQNSTRRILLL 367
LT+LNY PT A+ LP SPPP TP+WND K F+ +I A SP+PP RRI LL
Sbjct: 312 QGLTILNYKPTSASKLPSSPPPVTPRWNDYVHSKTFSNKILARMGSPRPPTKYDRRIFLL 371
Query: 368 NTQNQINGAEKWALNNVSLSLPTTPYLGAIKFGLNQSFDQGTPPETFPENYDINIPPTNN 427
NTQN+ING KWALNNVSL+LP TPYLG+IK+GL +FDQ TPPE F +NYD+ +PP N
Sbjct: 372 NTQNKINGFTKWALNNVSLTLPATPYLGSIKYGLKNAFDQKTPPENFADNYDVMVPPINP 431
Query: 428 ATTVRNAIHRLDFGSTVDIVLQNANTMVKNVSEIHPWHLHGHDFWVLGFGEGKFSAADEK 462
T+ + + ++ STVD++LQNAN + NVSEIHPWHLHGHDFWVLG+GEGKFS DEK
Sbjct: 432 NTSTGSGAYIIELNSTVDLILQNANALSDNVSEIHPWHLHGHDFWVLGYGEGKFSEKDEK 491
BLAST of Spg022862 vs. ExPASy TrEMBL
Match:
A0A6J1ENQ7 (L-ascorbate oxidase OS=Cucurbita moschata OX=3662 GN=LOC111436167 PE=3 SV=1)
HSP 1 Score: 542.0 bits (1395), Expect = 2.5e-150
Identity = 287/569 (50.44%), Postives = 351/569 (61.69%), Query Frame = 0
Query: 2 RDFRVLLVILYVLVSKIGWSQD-SQIRNFQWDVEYMFWSPDCEENVVMGINDQFPGPTIK 61
R+ L++ + L+ G S + SQIR+++W+VEYMFW+PDC EN+VMGIN QFPGPTI+
Sbjct: 8 REPNFLVLFFFGLILGFGISSEGSQIRHYKWEVEYMFWAPDCNENIVMGINGQFPGPTIR 67
Query: 62 ANVGDTVVVNVTNKLFTEG----------------------------------------- 121
AN GDTVVV +TNKL TEG
Sbjct: 68 ANAGDTVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFFYNFTVDN 127
Query: 122 ---------------------------------------RNILLS--W------------ 181
N+LLS W
Sbjct: 128 PGTFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLS 187
Query: 182 ---TLWDAK------NGR------TASKYNSSSSMKQCEFKGNEQCAPSILQVLPNKTYR 241
W + NGR A+KY+ S+++ C+ KG+E CAP I V P KTYR
Sbjct: 188 SKPIRWIGEPQTILLNGRGQFDCSIAAKYD--SNLEPCKLKGSEPCAPYIFHVSPKKTYR 247
Query: 242 LRIASTTSLAFLNVAIGVESQHDSSRADGNYVEPFTTSNINIYSGESYSVLITTNQDPTK 301
+RIASTT+LA LN AIG Q ADGNYV+PF TS+I+IYSGESYSVLITT+Q+P++
Sbjct: 248 IRIASTTALAALNFAIG-NHQLLVVEADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSE 307
Query: 302 NYWLSAAVTGQQPNTSSALTLLNYLPTPATDLPISPPPPTPQWNDTTRLKNFAKQISATA 361
NYW+S + PNT LTLLNYLP + LP SPPP TP W+D R KNF +I+A
Sbjct: 308 NYWVSVGTRARHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNFTYRITAAM 367
Query: 362 TSPKPPQNSTRRILLLNTQNQINGAEKWALNNVSLSLPTTPYLGAIKFGLNQSFDQGTPP 421
SPKPP RRI LLNTQN ING KWA+N+VSL+LP TPYLGA+K+ L +FDQ PP
Sbjct: 368 GSPKPPVKFNRRIFLLNTQNVINGYVKWAINDVSLALPPTPYLGAMKYNLLHAFDQNPPP 427
Query: 422 ETFPENYDINIPPTNNATTVRNAIHRLDFGSTVDIVLQNANTMVKNVSEIHPWHLHGHDF 461
E FPE+YDI+ PPTN T + N +++ G VD++LQNAN M +N+SE HPWHLHGHDF
Sbjct: 428 EVFPEDYDIDTPPTNEKTRIGNGVYQFKIGEVVDVILQNANMMKENLSETHPWHLHGHDF 487
BLAST of Spg022862 vs. ExPASy TrEMBL
Match:
A0A6J5XR80 (L-ascorbate oxidase OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCUS36932 PE=3 SV=1)
HSP 1 Score: 538.5 bits (1386), Expect = 2.7e-149
Identity = 281/567 (49.56%), Postives = 346/567 (61.02%), Query Frame = 0
Query: 5 RVLLVILYVLVSKIGWSQDSQIRNFQWDVEYMFWSPDCEENVVMGINDQFPGPTIKANVG 64
R++++ + V + + S+ S+ R+++WDVEY+FWSPDC ENVVMGIN QFPGPTI+A G
Sbjct: 6 RLIILSVLVCLLLVEASEGSKARHYKWDVEYLFWSPDCVENVVMGINGQFPGPTIRAKAG 65
Query: 65 DTVVVNVTNKLFTEGRNILLSW-------TLW---------------------------- 124
D VVV +TNKL TEG +++ W T W
Sbjct: 66 DMVVVELTNKLHTEG--VVIHWHGIRQFGTPWADGTASISQCAINPGETFIYRFKVDKAG 125
Query: 125 -------------------------DAK-------------------------------- 184
D K
Sbjct: 126 TYFYHGHFGMQRSAGLYGSLIVDVEDGKKEPFHYDGEFDLLLSDWWHQSVHHQEVGLSSK 185
Query: 185 -------------NGR-----TASKYNSSSSMKQCEFKGNEQCAPSILQVLPNKTYRLRI 244
NGR + + + S+SS QC +G+EQCAP IL VLPNK YRLRI
Sbjct: 186 PFRWIGEPQTLLINGRGQYNCSLAAHYSNSSSSQCRLRGSEQCAPPILHVLPNKIYRLRI 245
Query: 245 ASTTSLAFLNVAIGVESQHDSSRADGNYVEPFTTSNINIYSGESYSVLITTNQDPTKNYW 304
ASTT+LA LN+AIG + ADGNYV+PF +++IYSGESYSVLITTNQDP+ NYW
Sbjct: 246 ASTTALASLNLAIG-NHKMVVVEADGNYVQPFAVDDLDIYSGESYSVLITTNQDPSNNYW 305
Query: 305 LSAAVTGQQPNTSSALTLLNYLPTPATDLPISPPPPTPQWNDTTRLKNFAKQISATATSP 364
LS V G++P T LT+LNY P A+ LP S PP TP WND K+F +I A+ SP
Sbjct: 306 LSVGVRGREPKTQQGLTILNYHPNSASKLPASSPPVTPLWNDYNHSKSFTNKIFASMGSP 365
Query: 365 KPPQNSTRRILLLNTQNQINGAEKWALNNVSLSLPTTPYLGAIKFGLNQSFDQGTPPETF 424
KPP+N RRI LLNTQN++NG KWA+NNVSL+LP TPYLG+IK GL +F+Q PPE+F
Sbjct: 366 KPPRNYDRRITLLNTQNKLNGYTKWAINNVSLTLPPTPYLGSIKHGLRNAFNQENPPESF 425
Query: 425 PENYDINIPPTNNATTVRNAIHRLDFGSTVDIVLQNANTMVKNVSEIHPWHLHGHDFWVL 462
+YD+ PP N TT N ++ L +TVD++LQNAN + NVSEIHPWHLHGHDFWVL
Sbjct: 426 SNDYDVMKPPVNPNTTTGNGVYMLGMNTTVDVILQNANALSANVSEIHPWHLHGHDFWVL 485
BLAST of Spg022862 vs. ExPASy TrEMBL
Match:
A0A6J5V4G8 (L-ascorbate oxidase OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS37329 PE=3 SV=1)
HSP 1 Score: 537.7 bits (1384), Expect = 4.7e-149
Identity = 281/567 (49.56%), Postives = 346/567 (61.02%), Query Frame = 0
Query: 5 RVLLVILYVLVSKIGWSQDSQIRNFQWDVEYMFWSPDCEENVVMGINDQFPGPTIKANVG 64
R++++ + V + + S+ S+ R+++WDVEY+FWSPDC ENVVMGIN QFPGPTI+A G
Sbjct: 6 RLIILSVLVCLLLVEASEGSKARHYKWDVEYLFWSPDCVENVVMGINGQFPGPTIRAKAG 65
Query: 65 DTVVVNVTNKLFTEGRNILLSW-------TLW---------------------------- 124
D VVV +TNKL TEG +++ W T W
Sbjct: 66 DMVVVELTNKLHTEG--VVIHWHGIRQFGTPWADGTASISQCAINPGETFIYRFKVDKAG 125
Query: 125 -------------------------DAK-------------------------------- 184
D K
Sbjct: 126 TYFYHGHFGMQRSAGLYGSLIVDVEDGKKEPFHYDGEFDLLLSDWWHQSVHHQEVGLSSK 185
Query: 185 -------------NGR-----TASKYNSSSSMKQCEFKGNEQCAPSILQVLPNKTYRLRI 244
NGR + + + S+SS QC +G+EQCAP IL VLPNK YRLRI
Sbjct: 186 PFRWIGEPQTLLINGRGQYNCSLAAHYSNSSSSQCRLRGSEQCAPPILHVLPNKIYRLRI 245
Query: 245 ASTTSLAFLNVAIGVESQHDSSRADGNYVEPFTTSNINIYSGESYSVLITTNQDPTKNYW 304
ASTT+LA LN+AIG + ADGNYV+PF +++IYSGESYSVLITTNQDP+ NYW
Sbjct: 246 ASTTALASLNLAIG-NHKMVVVEADGNYVQPFAVDDLDIYSGESYSVLITTNQDPSNNYW 305
Query: 305 LSAAVTGQQPNTSSALTLLNYLPTPATDLPISPPPPTPQWNDTTRLKNFAKQISATATSP 364
LS V G++P T LT+LNY P A+ LP S PP TP WND K+F +I A+ SP
Sbjct: 306 LSVGVRGREPKTQQGLTILNYHPNSASKLPASSPPVTPLWNDYNHSKSFTNKIFASMGSP 365
Query: 365 KPPQNSTRRILLLNTQNQINGAEKWALNNVSLSLPTTPYLGAIKFGLNQSFDQGTPPETF 424
KPP+N RRI LLNTQN++NG KWA+NNVSL+LP TPYLG+IK GL +F+Q PPE+F
Sbjct: 366 KPPRNYDRRITLLNTQNKLNGYTKWAINNVSLTLPPTPYLGSIKNGLRNAFNQENPPESF 425
Query: 425 PENYDINIPPTNNATTVRNAIHRLDFGSTVDIVLQNANTMVKNVSEIHPWHLHGHDFWVL 462
+YD+ PP N TT N ++ L +TVD++LQNAN + NVSEIHPWHLHGHDFWVL
Sbjct: 426 SNDYDVMKPPVNPNTTTGNGVYMLGMNTTVDVILQNANALSANVSEIHPWHLHGHDFWVL 485
BLAST of Spg022862 vs. TAIR 10
Match:
AT5G21105.1 (Plant L-ascorbate oxidase )
HSP 1 Score: 489.6 bits (1259), Expect = 2.8e-138
Identity = 253/560 (45.18%), Postives = 338/560 (60.36%), Query Frame = 0
Query: 11 LYVLVSKIGWSQDSQIRNFQWDVEYMFWSPDCEENVVMGINDQFPGPTIKANVGDTVVVN 70
+ ++V+ + + + +R + W+VEY +WSPDC+E VM +N +FPGPTIKA GDT+VVN
Sbjct: 22 IVLVVAVLTHTASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTIVVN 81
Query: 71 VTNKLFTEGR-------------------------------------------------- 130
+TNKL TEG
Sbjct: 82 LTNKLTTEGLVIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFYHGHY 141
Query: 131 ------------------------------NILLS--W---------------TLWDAK- 190
N+LLS W W +
Sbjct: 142 GMQRSAGLYGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSSKPMRWIGEA 201
Query: 191 -----NGR------TASKYNSSSSMKQCEFKGNEQCAPSILQVLPNKTYRLRIASTTSLA 250
NGR A+++++++S+ C FK +QCAP IL V PNKTYR+R++STT+LA
Sbjct: 202 QSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALA 261
Query: 251 FLNVAIGVESQHDSSRADGNYVEPFTTSNINIYSGESYSVLITTNQDPTKNYWLSAAVTG 310
LN+A+ + ADGNY+ PFTT +I+IYSGESYSVL+TT+QDP++NY++S V G
Sbjct: 262 SLNLAV-QGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRG 321
Query: 311 QQPNTSSALTLLNYLPTPATDLPISPPPPTPQWNDTTRLKNFAKQISATATSPKPPQNST 370
++PNT+ ALT+LNY+ PA+ LP SPPP TP+W+D R KNF+K+I + SP PP+
Sbjct: 322 RKPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPPKKYR 381
Query: 371 RRILLLNTQNQINGAEKWALNNVSLSLPTTPYLGAIKFGLNQSFDQGTPPETFPENYDIN 430
+R++LLNTQN I+G KWA+NNVSL P TPYLG++K+ L F++ +PP ++ +YDI
Sbjct: 382 KRLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYDIM 441
Query: 431 IPPTNNATTVRNAIHRLDFGSTVDIVLQNANTMVKNVSEIHPWHLHGHDFWVLGFGEGKF 461
PP TT N I+ F TVD+++QNAN + VSEIHPWHLHGHDFWVLG+G+GKF
Sbjct: 442 NPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKF 501
BLAST of Spg022862 vs. TAIR 10
Match:
AT5G21105.2 (Plant L-ascorbate oxidase )
HSP 1 Score: 449.5 bits (1155), Expect = 3.2e-126
Identity = 209/369 (56.64%), Postives = 276/369 (74.80%), Query Frame = 0
Query: 93 NGRTASKYNSSSSMKQCEFKGNEQCAPSILQVLPNKTYRLRIASTTSLAFLNVAIGVESQ 152
N A+++++++S+ C FK +QCAP IL V PNKTYR+R++STT+LA LN+A+ +
Sbjct: 22 NCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALASLNLAV-QGHK 81
Query: 153 HDSSRADGNYVEPFTTSNINIYSGESYSVLITTNQDPTKNYWLSAAVTGQQPNTSSALTL 212
ADGNY+ PFTT +I+IYSGESYSVL+TT+QDP++NY++S V G++PNT+ ALT+
Sbjct: 82 LVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGRKPNTTQALTI 141
Query: 213 LNYLPTPATDLPISPPPPTPQWNDTTRLKNFAKQISATATSPKPPQNSTRRILLLNTQNQ 272
LNY+ PA+ LP SPPP TP+W+D R KNF+K+I + SP PP+ +R++LLNTQN
Sbjct: 142 LNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPPKKYRKRLILLNTQNL 201
Query: 273 INGAEKWALNNVSLSLPTTPYLGAIKFGLNQSFDQGTPPETFPENYDINIPPTNNATTVR 332
I+G KWA+NNVSL P TPYLG++K+ L F++ +PP ++ +YDI PP TT
Sbjct: 202 IDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYDIMNPPPFPNTTTG 261
Query: 333 NAIHRLDFGSTVDIVLQNANTMVKNVSEIHPWHLHGHDFWVLGFGEGKFS-AADEKQLNL 392
N I+ F TVD+++QNAN + VSEIHPWHLHGHDFWVLG+G+GKF DEK NL
Sbjct: 262 NGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFKPGIDEKTYNL 321
Query: 393 KNPALKNTVVVFPYGWIVVRFVADNPGVWIFHCHIEPHLHMGMGVVLAEGVDRVGDIPKN 452
KNP L+NT +++PYGW +RFV DNPGVW FHCHIEPHLHMGMGVV AEG++R+G +P
Sbjct: 322 KNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRIGKVPDE 381
Query: 453 ALSCGLTGQ 461
AL CGLT Q
Sbjct: 382 ALGCGLTKQ 389
BLAST of Spg022862 vs. TAIR 10
Match:
AT5G21100.1 (Plant L-ascorbate oxidase )
HSP 1 Score: 449.1 bits (1154), Expect = 4.2e-126
Identity = 245/538 (45.54%), Postives = 308/538 (57.25%), Query Frame = 0
Query: 31 WDVEYMFWSPDCEENVVMGINDQFPGPTIKANVGDTVVVNVTNKLFTEG----------- 90
W+VEY +W PDC+E +VM IN QFPGPTI A GDTV+++V NKL TEG
Sbjct: 28 WEVEYKYWWPDCKEGIVMAINGQFPGPTIDAVAGDTVIIHVVNKLSTEGVVIHWHGIRQK 87
Query: 91 ------------------------------------------------------------ 150
Sbjct: 88 GTPWADGAAGVTQCPINPGETFTYKFIVDKAGTHFYHGHYGMQRSSGLYGMLIVRSPKER 147
Query: 151 ------RNILLS--W---------------TLWDAK------NGR------TASKYNSSS 210
N+LLS W W + NGR A+ +N
Sbjct: 148 LIYDGEFNLLLSDWWHQSIHAQELALSSRPMRWIGEPQSLLINGRGQFNCSQAAYFNKGG 207
Query: 211 SMKQCEFKGNEQCAPSILQVLPNKTYRLRIASTTSLAFLNVAIGVESQHDSSRADGNYVE 270
C FK N+QCAP L+V PN+ YRLRIASTT+LA LN+A+ Q ADGNYV
Sbjct: 208 EKDVCTFKENDQCAPQTLRVEPNRVYRLRIASTTALASLNLAV-QGHQLVVVEADGNYVA 267
Query: 271 PFTTSNINIYSGESYSVLITTNQDPTKNYWLSAAVTGQQPNTSSALTLLNYLPTPATDLP 330
PFT ++I++YSGE+YSVL+ TN P+K YW+S V G++P T ALT++NY+ P
Sbjct: 268 PFTVNDIDVYSGETYSVLLKTNALPSKKYWISVGVRGREPKTPQALTVINYVDA-TESRP 327
Query: 331 ISPPPPTPQWNDTTRLKNFAKQISATATSPKPPQNSTRRILLLNTQNQINGAEKWALNNV 390
PPP TP WNDT R K+F+K+I A PKPP+ S +++LLNTQN KW++NNV
Sbjct: 328 SHPPPVTPIWNDTDRSKSFSKKIFAAKGYPKPPEKSHDQLILLNTQNLYEDYTKWSINNV 387
Query: 391 SLSLPTTPYLGAIKFGLNQSFDQGTP-PETFPENYDINIPPTNNATTVRNAIHRLDFGST 450
SLS+P TPYLG+I++GL ++D +P + +NYDI PP N TT + I+ FG
Sbjct: 388 SLSVPVTPYLGSIRYGLKSAYDLKSPAKKLIMDNYDIMKPPPNPNTTKGSGIYNFAFGIV 447
Query: 451 VDIVLQNANTMVKNVSEIHPWHLHGHDFWVLGFGEGKFS-AADEKQLNLKNPALKNTVVV 459
VD++LQNAN + +SEIHPWH+HGHDFWVLG+GEGKF DEK NLKNP L+NTVV+
Sbjct: 448 VDVILQNANVLKGVISEIHPWHIHGHDFWVLGYGEGKFKPGIDEKTFNLKNPPLRNTVVL 507
BLAST of Spg022862 vs. TAIR 10
Match:
AT5G21105.3 (Plant L-ascorbate oxidase )
HSP 1 Score: 416.8 bits (1070), Expect = 2.3e-116
Identity = 222/518 (42.86%), Postives = 303/518 (58.49%), Query Frame = 0
Query: 11 LYVLVSKIGWSQDSQIRNFQWDVEYMFWSPDCEENVVMGINDQFPGPTIKANVGDTVVVN 70
+ ++V+ + + + +R + W+VEY +WSPDC+E VM +N +FPGPTIKA GDT+VVN
Sbjct: 22 IVLVVAVLTHTASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTIVVN 81
Query: 71 VTNKLFTEGR-------------------------------------------------- 130
+TNKL TEG
Sbjct: 82 LTNKLTTEGLVIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFYHGHY 141
Query: 131 ------------------------------NILLS--W---------------TLWDAK- 190
N+LLS W W +
Sbjct: 142 GMQRSAGLYGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSSKPMRWIGEA 201
Query: 191 -----NGR------TASKYNSSSSMKQCEFKGNEQCAPSILQVLPNKTYRLRIASTTSLA 250
NGR A+++++++S+ C FK +QCAP IL V PNKTYR+R++STT+LA
Sbjct: 202 QSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALA 261
Query: 251 FLNVAIGVESQHDSSRADGNYVEPFTTSNINIYSGESYSVLITTNQDPTKNYWLSAAVTG 310
LN+A+ + ADGNY+ PFTT +I+IYSGESYSVL+TT+QDP++NY++S V G
Sbjct: 262 SLNLAV-QGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRG 321
Query: 311 QQPNTSSALTLLNYLPTPATDLPISPPPPTPQWNDTTRLKNFAKQISATATSPKPPQNST 370
++PNT+ ALT+LNY+ PA+ LP SPPP TP+W+D R KNF+K+I + SP PP+
Sbjct: 322 RKPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPPKKYR 381
Query: 371 RRILLLNTQNQINGAEKWALNNVSLSLPTTPYLGAIKFGLNQSFDQGTPPETFPENYDIN 419
+R++LLNTQN I+G KWA+NNVSL P TPYLG++K+ L F++ +PP ++ +YDI
Sbjct: 382 KRLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYDIM 441
BLAST of Spg022862 vs. TAIR 10
Match:
AT4G39830.1 (Cupredoxin superfamily protein )
HSP 1 Score: 350.1 bits (897), Expect = 2.7e-96
Identity = 218/570 (38.25%), Postives = 289/570 (50.70%), Query Frame = 0
Query: 7 LLVILYVLVSKIGWSQDSQIRNFQWDVEYMFWSPDCEENVVMGINDQFPGPTIKANVGDT 66
L+V+ ++ + +IR F+W+V+Y F SPDC E +V+ IN +FPGPTIKA GDT
Sbjct: 16 LMVLCFIALFFSSVLCQGKIRRFKWEVKYEFKSPDCFEKLVITINGKFPGPTIKAQQGDT 75
Query: 67 VVVNVTNKLFTEGRNILLSW-------TLW-DAKNGRT---------------------- 126
+VV + N TE N+ + W T W D G T
Sbjct: 76 IVVELKNSFMTE--NVAVHWHGIRQIGTPWFDGVEGVTQCPILPGEVFIYQFVVDRPGTY 135
Query: 127 ----------------------------------------ASKYNSSSSMK-----QCEF 186
Y+ S S K F
Sbjct: 136 MYHSHYGMQRESGLIGMIQVSPPATEPEPFTYDYDRNFLLTDWYHKSMSEKATGLASIPF 195
Query: 187 K-----------------------------------GNEQCAPSILQVLPNKTYRLRIAS 246
K N C+ IL V+P KTYRLRI S
Sbjct: 196 KWVGEPQSLMIQGRGRFNCSNNLTTPPSLVSGVCNVSNADCSRFILTVIPGKTYRLRIGS 255
Query: 247 TTSLAFLNVAIGVESQHDSS--RADGNYVEPFTTSNINIYSGESYSVLITTNQDPTKNYW 306
T+L+ L+ I H+ + ADG+YVEPFT N+ +YSGE+YSVL+ +Q+P +NYW
Sbjct: 256 LTALSALSFQI---EGHNLTVVEADGHYVEPFTVKNLFVYSGETYSVLLKADQNPRRNYW 315
Query: 307 LSAAVTGQQPNTSSALTLLNYLPT-PATDLPISPPPP-TPQWNDT-TRLKNFAKQISATA 366
+++++ + T A +LNY P P P S P+WNDT +RL +
Sbjct: 316 ITSSIVSRPATTPPATAVLNYYPNHPRRRPPTSESSNIVPEWNDTRSRLAQSLAIKARRG 375
Query: 367 TSPKPPQNSTRRILLLNTQNQINGAEKWALNNVSLSLPTTPYLGAIKFGLNQSFD-QGTP 426
P+NS + I+LLNTQN++NG +W++NNVS P TPYL A+K L +FD + T
Sbjct: 376 FIHALPENSDKVIVLLNTQNEVNGYRRWSVNNVSYHHPKTPYLIALKQNLTNAFDWRFTA 435
Query: 427 PETF-PENYDINIPPTNNATTVRNAIHRLDFGSTVDIVLQNANTMVKNVSEIHPWHLHGH 459
PE + NYDI P N T + I+RL F STVD++LQNANTM N SE HPWHLHGH
Sbjct: 436 PENYDSRNYDIFAKPLNANATTSDGIYRLRFNSTVDVILQNANTMNANNSETHPWHLHGH 495
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| P24792 | 3.0e-153 | 50.44 | L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2 | [more] |
| P14133 | 1.8e-150 | 52.20 | L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1 | [more] |
| P37064 | 3.1e-150 | 51.10 | L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1 | [more] |
| Q40588 | 4.7e-138 | 47.18 | L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1 | [more] |
| M4DUF2 | 4.1e-134 | 45.60 | L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1HPZ9 | 1.1e-150 | 50.44 | L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=LOC111466657 PE=3 SV=1 | [more] |
| A0A660KQX3 | 1.5e-150 | 51.08 | Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_010271 PE=3 SV=1 | [more] |
| A0A6J1ENQ7 | 2.5e-150 | 50.44 | L-ascorbate oxidase OS=Cucurbita moschata OX=3662 GN=LOC111436167 PE=3 SV=1 | [more] |
| A0A6J5XR80 | 2.7e-149 | 49.56 | L-ascorbate oxidase OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCUS36932 PE=3 SV... | [more] |
| A0A6J5V4G8 | 4.7e-149 | 49.56 | L-ascorbate oxidase OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS37329 PE=3 SV=1 | [more] |
