Spg022823 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg022823
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Replication protein A 32 kDa subunit B-like
Location	scaffold2: 2309798 .. 2314385 (+)
RNA-Seq Expression	Spg022823
Synteny	Spg022823

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGAAATGGCGGGAGCCTGGAGGGAAGCTGAACTGGTATTTCTAGGTTTTAACAAAATAGAATTTCATTTCGAAAACTGCTCCTCAAATTTGCTCTCCATCAGTCACTTGATGCTCTGCTCTTACAGGGTTTGAATACTATTCAGTTGTAGCGTTACAGTGGAAGAATGATGTATGGAAGTCAATTCGACGGAAACGCTGCCTTCTCCGGTGGCGGATTCATGCCATCTCAGACCACCCAGGCCCCTGATCATTCCTTCTCTCCGGCGAAAGTACGTTCTTTCTCCAGATATTATATCATCTCTCTTCCGGATTCCTTTAATTTCTGTATGGATTACTAGTCTCCGCTGTTTAGATTTATCACTTGCGGTTTCATGTATTTTCTGATTGAATTCGTTCAAGTGGTTTGAAATTATAAGTACTTTGATAGCCAATTAGGTGGATAGTAATTCATTTATCTCTTGGATTTGACGTCTAGTTCATATAATTGAAGTATGGTTGATATAATGGTGTTTTTGCCCTTACGGTTTCCGCGGACAATAGTTTCTTCTCCTCACGGTCGCTGGGAAAGTTAAGTTGAATTTGTATTTCTTCGTCTATTGGAATAATATAGAAGAAGTACTGTTCCTTTGTGTCATAAGTTATTTAAATGGCGAAAATGCTTTGGATTCACTGGTTTTCACCTTTTGTATATCCTCAAGAATGTTTATTCTCAACACAATGAAGTCTTACGTTTTCGTGTCTAATTTGCAGAATCGAGATGTTCAGGCATTGCTTCCGTTAACAGTGAAGCAGATAAACAACGCATTCCTGTCTAGTGATGATAAATCAAATTTTGTTATTGATGGTGTTGATGTTAATAATGTTAGTTATCGAACTTGTATGTTTTGTCTATTTTTAGTTGTTGAGTTGCTTGTTGAGCTGCATTATGTTCAGGTTAAGCTTATTGGGATGGTGCGCAATAGAGCAGGAAGAATTACTGATGTGACTTTTGCACTGGATGATGGCACTGGACGGATTGACTGTAGCAAATGGTTTGTTCTCCCCTCCTCAAAGATGGAAAAGAAGTTAACCATTGTTGATATTGATTCTTTTTGATGAATCTATAGGGTCAATGAAGCTGCGGATTCGAATGAAGTTGAAGGAATATTGTAATGTTCTTTTTACCCTGTCTTAATAGCATTTAAAATATTCCCTCAAAATTATAATACTTTTTGTGCCGTTATTATTTTCATGATTAGTTTTTCAGTGTTGCCTAGAATCTATTATTCTTTAGGATGGAAGTGGAATTTCAGAACATGACAATGTTATAGACATGAAGTTTGATGGGAGTTTTGTGGTTGGTTCTTCAGTTTTTCACACGGTTTTACTCGTTCTAAGAATTCTCTTATTAATTTTTAGTAAAATGAGATATATTGACTGAGTCCTAAGGAAAATTTGCAAACTAGCTGTTTTGAATTTAGCAATACGGAGTCAGTAATTCCCCACACAAATATTAGGATTTAAGTAAGCTTAAATCTGGTTAAATATGATTCAATATATAAACTACACCCTCACTCTGTGGCCTGGATAGATCTTTATGAGCATGCCTGTGAGGTTATGACCAAACCAGTAGTCTTATTCGTATATATGTGTAATTCATTGTTCTGTATTGTCCTATGGGTGTTTGTATATATTCTTTTTCCAGCAAAATGAACTATATACCTGTAGCCCATGCTACTATGGAGCATACAAATGTCTTATTTATCTCAGTCCTATTGAGATAATGAGTCACAAGATGGAGTGTACCTTTTTTTTTTCTTCTCTTATCAGATTCCCCATTACATATCACAGTTTTTTTTATCATGATTTAAACAAATGATTTCTAACCAGGACATGATTATGTGGAAATTATAACTTGTTGCATGGCTTGAACATATTTTGGATTCCATTTTCAGCTCTTTTACTCTCTTGAAATGTGAAATTGTGAATGATCGGTTCGAAATGGACTAATCAGTCTTTCTATGTGTATCATGACACTAATTTTAGCATATAAAGAAAGAGAGATGGCTGGCTAGATCATATTCACTTCCTTAAGATTTCCATCAAGTTGCAATTATCATTCGTTGAAATATCTCTTGTGGCTCTGCTGGATTATGATTTGTGTTTAACCTGAAATTAATTCTAGAGGGTTTTGGATGATTTGGGGGATGGCTCTGCTTATTTGGACTAACTGATGCATTGAGTCATCTCTGAAGTTTATCGCCTTTTAGCTCATGCCACTTAGATAAGATTCTTGTGCCTCAGGGATGGCATGTATGTCCGGGTCCATGGCCACTTGAAAAGCTTCCAGGGTAAAAGAACTTTGAATGTCTTTTCTATCAGGTATATTATAGTTTTCCTTTGTTCTTTTTGAGGGGTCTCCTGGTTTTGTTATTTCAGAGGGATTTAAGTTGTGAGTGCACAAACTGTATTAATGCAATTCTTGAACTATAGTGGATAGGAGTTTATGGTTATATTTTTAGCAATTGAATTGAAACGAGGCACATTCTTAAATGAAAAATTAATCATGATTGTTCTATTGTTGAAATTTTGATTATTTTGTAACTTAAACATTGCACGAGCAGTTCTTTTTCACAACATGAAACTTGTGTATTAGATTTCTATTGCTGTGGGGGGATTTTGATATAAAATATTTGGTTTCCAGGCCTGTGACCGACTACAATGAGATCACAAGTCACTTCATCGAATGCATATATGTTCATTTTTACAATACTAGATTACGGGTATTAATCTAGCCGTTCAAATTTTACTTGATATTTTGATTAGGTGAAAAGTAATATTTATGGTCTTACCTGCTGCTTCTTTTGGGTTATGTTTATATTGTGCTGATAGAAACAACAAAGTAGCAGTACGACTACTCAGCCACAGATGCCGAATGCATCTAATACGCCTATGAAAGGATATCAAGCTCCCATTCCAAATCAAGTAAGATTCTGGTTCAATTCTCATATGATGATGAGCTACAGTTTGTTTATACTATATGCATCCTTCTGATGATCCTTGATTCCTTTTTCAGTACTCTGGTCAAGCAGGTGGTGATGGTTGGAAAAGCGTTGAGCAGATGGTTTTAGATTTTTTGCAACTTCCTTCATGCGAGTGAGTGCCTTTTAACAATGAGCAAATCTTTTCATTTCATATTCCCATCTTTTATTTCCATGCTTGTTTGTCTTGAATTTGTTATTTTGTCAGATGCATAGTTATGAAATGTGATTTTTTCTAAGGCTTTTCCTTATATTTCTTTTGGTTTTGATGATTGATGTGTGCTCTTTCTCTATCTTTTTGTTTCTCTCTCTTTCATGAATAGATGACTGACTTCTTGAGTGGAGACTCGACCTCCATAGCTTGGCGAATTGTTTACTATTACTTATTAGGATCCTCATATCACTCAAGCACTCTTGTTTTAATGCCTACAAATCACTGAAGCACTTATAGAGAAGGTTGTCCCTAGATGAGATTTACTGAGAAAAGTTCTTGTCCTAATCTACTTAATTGGTATTTCCTTGTTCGGGAGTAGAGAATCAGAATACCTAATCAAGTATAGGATGGTACAACGTTAAAAATATTTGTCTCACTATAAGCAACAATCTATAGTGTAAAATGATGCATAAGCTATGTTGATTGTCATGATGGACCCTGCATATTTTGAGCGAGGGTAGTGATAACTAGACAATTTATTGTAGTTTCTTTATCCCGTGTCCTTTGGGTTCATGTTGCGCTTTATTCTTTCTTCTTTTTTTTTCCCCTTTTTTTGGTGTGTTATAGCAAAGCATGAAACGTCATTTGCCAAACAGATTACATGATATTGGGATGGCCAATCCATATTTCCTTGGTATGATTGCCTTGGTCCATATTGATCTCTTTCTGCAATTGATGAGTGTTCTGTATTTTTGGGTGAAAAAACAAAGTCTGGAAATTCGAATGCTCCCTCACTATTTTTTTTCCCTGCTCATCTATTTCTTAGTTTTAATTTTTCATTGGAAGCTATATTCAAACTTTTCTTGTGTTTAGTAATGAGAGGGGCGCACACCGTGATGTCATTGCTCAACAACTTAAAGTTCCACTAGAGAAGCTGCTGTATTACTCTCTTTCCCTCACCTCTACTTGACAGCAGCACTTACCTTGCAAGTAGGGTTAACTTAATATACCATTTTGCTTGTTTTTTTGCAGTCCAGCGATGAAAAATCTAGAAGAGGAAGGCCTAATCTACTCAACAACTGATGATTTCCACTTCAAATCAACCGCCAACGGTTGAATTCTCTATTATGGTGTAACTAATGAATTGAAGGAGGCAAGTTTATTTTCTGTCGTTCTTTCTTTTCATTTGTGGTTTAAGTTGGGAAAATCTTTGTAGTCAAAATATGATCCGTGCAGATCTGTTTTTGCCTTTATATTTAACTTGCCAGCGAACAAAAAAAGATAAAATGAAAACAAAAAGCCCGGGGCCATGACTTGTTGAAACTATTGTTATGACATTTGCTTCTACTGTGCAACATTTTGAATGCACTAATACAGCACAGGGTGCAACCTGCAGAGGTGGATTTGGCTGA

mRNA sequence

ATGATGTATGGAAGTCAATTCGACGGAAACGCTGCCTTCTCCGGTGGCGGATTCATGCCATCTCAGACCACCCAGGCCCCTGATCATTCCTTCTCTCCGGCGAAAAATCGAGATGTTCAGGCATTGCTTCCGTTAACAGTGAAGCAGATAAACAACGCATTCCTGTCTAGTGATGATAAATCAAATTTTGTTATTGATGGTGTTGATGTTAATAATGTTAAGCTTATTGGGATGGTGCGCAATAGAGCAGGAAGAATTACTGATGTGACTTTTGCACTGGATGATGGCACTGGACGGATTGACTGTAGCAAATGGGTCAATGAAGCTGCGGATTCGAATGAAGTTGAAGGAATATTGGATGGCATGTATGTCCGGGTCCATGGCCACTTGAAAAGCTTCCAGGGTAAAAGAACTTTGAATGTCTTTTCTATCAGGCCTGTGACCGACTACAATGAGATCACAAGTCACTTCATCGAATGCATATATGTTCATTTTTACAATACTAGATTACGGAAACAACAAAGTAGCAGTACGACTACTCAGCCACAGATGCCGAATGCATCTAATACGCCTATGAAAGGATATCAAGCTCCCATTCCAAATCAATACTCTGGTCAAGCAGGTGGTGATGGTTGGAAAAGCGTTGAGCAGATGGTTTTAGATTTTTTGCAACTTCCTTCATGCGATAATGAGAGGGGCGCACACCGTGATGTCATTGCTCAACAACTTAAAGTTCCACTAGAGAAGCTGCTTCCAGCGATGAAAAATCTAGAAGAGGAAGGCCTAATCTACTCAACAACTGATGATTTCCACTTCAAATCAACCGCCAACGGTTGA

Coding sequence (CDS)

ATGATGTATGGAAGTCAATTCGACGGAAACGCTGCCTTCTCCGGTGGCGGATTCATGCCATCTCAGACCACCCAGGCCCCTGATCATTCCTTCTCTCCGGCGAAAAATCGAGATGTTCAGGCATTGCTTCCGTTAACAGTGAAGCAGATAAACAACGCATTCCTGTCTAGTGATGATAAATCAAATTTTGTTATTGATGGTGTTGATGTTAATAATGTTAAGCTTATTGGGATGGTGCGCAATAGAGCAGGAAGAATTACTGATGTGACTTTTGCACTGGATGATGGCACTGGACGGATTGACTGTAGCAAATGGGTCAATGAAGCTGCGGATTCGAATGAAGTTGAAGGAATATTGGATGGCATGTATGTCCGGGTCCATGGCCACTTGAAAAGCTTCCAGGGTAAAAGAACTTTGAATGTCTTTTCTATCAGGCCTGTGACCGACTACAATGAGATCACAAGTCACTTCATCGAATGCATATATGTTCATTTTTACAATACTAGATTACGGAAACAACAAAGTAGCAGTACGACTACTCAGCCACAGATGCCGAATGCATCTAATACGCCTATGAAAGGATATCAAGCTCCCATTCCAAATCAATACTCTGGTCAAGCAGGTGGTGATGGTTGGAAAAGCGTTGAGCAGATGGTTTTAGATTTTTTGCAACTTCCTTCATGCGATAATGAGAGGGGCGCACACCGTGATGTCATTGCTCAACAACTTAAAGTTCCACTAGAGAAGCTGCTTCCAGCGATGAAAAATCTAGAAGAGGAAGGCCTAATCTACTCAACAACTGATGATTTCCACTTCAAATCAACCGCCAACGGTTGA

Protein sequence

MMYGSQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINNAFLSSDDKSNFVIDGVDVNNVKLIGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDGMYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSTTTQPQMPNASNTPMKGYQAPIPNQYSGQAGGDGWKSVEQMVLDFLQLPSCDNERGAHRDVIAQQLKVPLEKLLPAMKNLEEEGLIYSTTDDFHFKSTANG

Homology

[Close]

BLAST of Spg022823 vs. NCBI nr
Match: XP_008453602.1 (PREDICTED: replication protein A 32 kDa subunit B-like [Cucumis melo] >XP_008453603.1 PREDICTED: replication protein A 32 kDa subunit B-like [Cucumis melo] >XP_016901361.1 PREDICTED: replication protein A 32 kDa subunit B-like [Cucumis melo] >KAA0058271.1 replication protein A 32 kDa subunit B-like [Cucumis melo var. makuwa] >TYK11823.1 replication protein A 32 kDa subunit B-like [Cucumis melo var. makuwa])

HSP 1 Score: 533.5 bits (1373), Expect = 1.1e-147
Identity = 261/277 (94.22%), Postives = 270/277 (97.47%), Query Frame = 0


Query: 2   MYGSQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINNAFLSSDDKS 61
           MY SQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQIN+AFLSSDDKS
Sbjct: 1   MYASQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINDAFLSSDDKS 60

Query: 62  NFVIDGVDVNNVKLIGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 121
           NFVIDGVDVNNVKL+GMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG
Sbjct: 61  NFVIDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 120

Query: 122 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSTTTQ 181
           MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEIT+HFIE IYVHFYNTRLRKQQSSS TTQ
Sbjct: 121 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQ 180

Query: 182 PQMPNASNTPMKGYQAPIPNQYSGQAGGDGWKSVEQMVLDFLQLPSCDNERGAHRDVIAQ 241
           PQM N SNTP+KGYQAPI NQY+GQAGGD WKS+EQMVLDFLQLPSCD+ERGAHRDVIAQ
Sbjct: 181 PQMTNLSNTPVKGYQAPIANQYTGQAGGDSWKSLEQMVLDFLQLPSCDSERGAHRDVIAQ 240

Query: 242 QLKVPLEKLLPAMKNLEEEGLIYSTTDDFHFKSTANG 279
           QLKVPLEKL+PAMKNLEEEGLIYSTTDD+HFKSTANG
Sbjct: 241 QLKVPLEKLIPAMKNLEEEGLIYSTTDDYHFKSTANG 277

[Close]

BLAST of Spg022823 vs. NCBI nr
Match: XP_011656845.1 (replication protein A 32 kDa subunit B [Cucumis sativus] >KAE8653166.1 hypothetical protein Csa_019848 [Cucumis sativus])

HSP 1 Score: 532.7 bits (1371), Expect = 1.9e-147
Identity = 262/277 (94.58%), Postives = 269/277 (97.11%), Query Frame = 0


Query: 2   MYGSQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINNAFLSSDDKS 61
           MY SQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQIN+AFLSSDDKS
Sbjct: 1   MYASQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINDAFLSSDDKS 60

Query: 62  NFVIDGVDVNNVKLIGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 121
           NFVIDGVDVNNVKL+GMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG
Sbjct: 61  NFVIDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 120

Query: 122 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSTTTQ 181
           MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEIT+HFIE IYVHFYNTRLRKQQSSS TTQ
Sbjct: 121 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQ 180

Query: 182 PQMPNASNTPMKGYQAPIPNQYSGQAGGDGWKSVEQMVLDFLQLPSCDNERGAHRDVIAQ 241
           PQM N SNTPMK YQAPI NQY+GQAGGD WKS+EQMVLDFLQLPSCD+ERGAHRDVIAQ
Sbjct: 181 PQMTNLSNTPMKVYQAPIANQYTGQAGGDSWKSLEQMVLDFLQLPSCDSERGAHRDVIAQ 240

Query: 242 QLKVPLEKLLPAMKNLEEEGLIYSTTDDFHFKSTANG 279
           QLKVPLEKL+PAMKNLEEEGLIYSTTDDFHFKSTANG
Sbjct: 241 QLKVPLEKLIPAMKNLEEEGLIYSTTDDFHFKSTANG 277

[Close]

BLAST of Spg022823 vs. NCBI nr
Match: XP_038880298.1 (replication protein A 32 kDa subunit B [Benincasa hispida])

HSP 1 Score: 528.9 bits (1361), Expect = 2.7e-146
Identity = 261/277 (94.22%), Postives = 269/277 (97.11%), Query Frame = 0


Query: 2   MYGSQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINNAFLSSDDKS 61
           MYGSQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQIN+AFLSSDDKS
Sbjct: 1   MYGSQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINDAFLSSDDKS 60

Query: 62  NFVIDGVDVNNVKLIGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 121
           NFVIDGVDVNNVKL+GMVRNRAGRITDVTFALDDGTG IDCSKWVNEAADSNEVEGILDG
Sbjct: 61  NFVIDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGWIDCSKWVNEAADSNEVEGILDG 120

Query: 122 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSTTTQ 181
           MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIE IYVHFYNTRLRKQQ SS  TQ
Sbjct: 121 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIESIYVHFYNTRLRKQQGSS-ITQ 180

Query: 182 PQMPNASNTPMKGYQAPIPNQYSGQAGGDGWKSVEQMVLDFLQLPSCDNERGAHRDVIAQ 241
           PQM N SNTPMKGYQAP+ NQY+GQAGGDGWKS+EQMVLDFLQLPSCD+ERGAHRDVIAQ
Sbjct: 181 PQMANLSNTPMKGYQAPMSNQYTGQAGGDGWKSLEQMVLDFLQLPSCDSERGAHRDVIAQ 240

Query: 242 QLKVPLEKLLPAMKNLEEEGLIYSTTDDFHFKSTANG 279
           QLKVPLEKL+PAMKNLEEEGLIYSTTDD+HFKSTANG
Sbjct: 241 QLKVPLEKLIPAMKNLEEEGLIYSTTDDYHFKSTANG 276

[Close]

BLAST of Spg022823 vs. NCBI nr
Match: XP_023516911.1 (replication protein A 32 kDa subunit B-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023516912.1 replication protein A 32 kDa subunit B-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 527.3 bits (1357), Expect = 7.9e-146
Identity = 257/277 (92.78%), Postives = 266/277 (96.03%), Query Frame = 0


Query: 2   MYGSQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINNAFLSSDDKS 61
           MYGSQFDGNAAFSGGGFMPSQTT APDHS SPAKNRDVQALLPLTVKQIN+AFLSSDDKS
Sbjct: 1   MYGSQFDGNAAFSGGGFMPSQTTMAPDHSLSPAKNRDVQALLPLTVKQINDAFLSSDDKS 60

Query: 62  NFVIDGVDVNNVKLIGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 121
           NFV+DGVDVNNVKL+GMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG
Sbjct: 61  NFVVDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 120

Query: 122 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSTTTQ 181
           MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIE IYVHFYNTRLRKQQSSS T Q
Sbjct: 121 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIESIYVHFYNTRLRKQQSSSMTPQ 180

Query: 182 PQMPNASNTPMKGYQAPIPNQYSGQAGGDGWKSVEQMVLDFLQLPSCDNERGAHRDVIAQ 241
           PQM N SNTP++GYQ PI NQYSGQAGGDGWK++EQMVLDFLQLPSCD ERGAHRDVIAQ
Sbjct: 181 PQMANLSNTPVRGYQTPISNQYSGQAGGDGWKNLEQMVLDFLQLPSCDTERGAHRDVIAQ 240

Query: 242 QLKVPLEKLLPAMKNLEEEGLIYSTTDDFHFKSTANG 279
            LKVPLEKL+PAMKNLEEEGLIYSTTDD+HFKSTANG
Sbjct: 241 HLKVPLEKLMPAMKNLEEEGLIYSTTDDYHFKSTANG 277

[Close]

BLAST of Spg022823 vs. NCBI nr
Match: XP_022988280.1 (replication protein A 32 kDa subunit B [Cucurbita maxima])

HSP 1 Score: 525.0 bits (1351), Expect = 3.9e-145
Identity = 255/277 (92.06%), Postives = 266/277 (96.03%), Query Frame = 0


Query: 2   MYGSQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINNAFLSSDDKS 61
           MYGSQFDGNAAFSGGGFMPSQTT APDHS SPAKNRDVQALLPLTVKQIN+AFLSSDDKS
Sbjct: 1   MYGSQFDGNAAFSGGGFMPSQTTMAPDHSLSPAKNRDVQALLPLTVKQINDAFLSSDDKS 60

Query: 62  NFVIDGVDVNNVKLIGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 121
           NFV+DGVDVNNVKL+GMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG
Sbjct: 61  NFVVDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 120

Query: 122 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSTTTQ 181
           MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIE IYVHFYNTRLRKQQSSS T Q
Sbjct: 121 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIESIYVHFYNTRLRKQQSSSMTPQ 180

Query: 182 PQMPNASNTPMKGYQAPIPNQYSGQAGGDGWKSVEQMVLDFLQLPSCDNERGAHRDVIAQ 241
           PQM N SNTP++GYQ P+ NQY+GQAGGDGWK++EQMVLDFLQLPSCD ERGAHRDVIAQ
Sbjct: 181 PQMANLSNTPVRGYQTPMSNQYTGQAGGDGWKNLEQMVLDFLQLPSCDTERGAHRDVIAQ 240

Query: 242 QLKVPLEKLLPAMKNLEEEGLIYSTTDDFHFKSTANG 279
            LKVPLEKL+PAMKNLEEEGLIYSTTDD+HFKSTANG
Sbjct: 241 HLKVPLEKLMPAMKNLEEEGLIYSTTDDYHFKSTANG 277

[Close]

BLAST of Spg022823 vs. ExPASy Swiss-Prot
Match: Q8LFJ8 (Replication protein A 32 kDa subunit B OS=Arabidopsis thaliana OX=3702 GN=RPA2B PE=2 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 6.0e-72
Identity = 142/280 (50.71%), Postives = 188/280 (67.14%), Query Frame = 0


Query: 2   MYGSQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINNAFLSSDDKS 61
           MYG  FDGNAAF+GGGFMPSQ T     S S  KNRDV+ LLPLT+KQ+++A  S+  +S
Sbjct: 1   MYGGDFDGNAAFAGGGFMPSQATTQAHESSSSLKNRDVRTLLPLTLKQLSSA--STTGES 60

Query: 62  NFVIDGVDVNNVKLIGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 121
           NF IDGVD+  V ++G +     RIT V F +DDGTG +DC +W +   ++ E+E +  G
Sbjct: 61  NFSIDGVDIKTVVIVGRISRMENRITQVDFVVDDGTGWVDCVRWCHARQETEEMEAVKLG 120

Query: 122 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLR--KQQSSSTT 181
           MYVR+HGHLK FQGKR++NVFS+RPVTD+NEI  HF EC+YVH YNT+LR       + T
Sbjct: 121 MYVRLHGHLKIFQGKRSVNVFSVRPVTDFNEIVHHFTECMYVHMYNTKLRGGSITQDTAT 180

Query: 182 TQPQMP-NASNTPMKGYQAPIPNQYSGQAGGDGWKSVEQMVLDFLQLP-SCDNERGAHRD 241
            +PQMP +   TP K YQ    NQ+  Q   D    V+Q VL++L  P    +E G H D
Sbjct: 181 PRPQMPYSTMPTPAKPYQTGPSNQFPNQF-NDSMHGVKQTVLNYLNQPMHIVSEAGVHCD 240

Query: 242 VIAQQLKVPLEKLLPAMKNLEEEGLIYSTTDDFHFKSTAN 278
           +IA++L++PL ++  A++ L  +G IYST D+  FKSTAN
Sbjct: 241 IIARELRIPLLQVKEALEQLSNDGCIYSTLDETCFKSTAN 277

[Close]

BLAST of Spg022823 vs. ExPASy Swiss-Prot
Match: Q9ZQ19 (Replication protein A 32 kDa subunit A OS=Arabidopsis thaliana OX=3702 GN=RPA2A PE=1 SV=2)

HSP 1 Score: 259.2 bits (661), Expect = 5.2e-68
Identity = 132/277 (47.65%), Postives = 186/277 (67.15%), Query Frame = 0


Query: 1   MMYGSQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINNAFLSSDDK 60
           M   SQF+ N+ FSGGGFM SQ +QA + S S AKNRD Q L+P+TVKQI   F SS +K
Sbjct: 1   MFSSSQFEPNSGFSGGGFMSSQPSQAYESSSSTAKNRDFQGLVPVTVKQITECFQSSGEK 60

Query: 61  SNFVIDGVDVNNVKLIGMVRNR-AGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGIL 120
           S  VI+G+ + NV L+G+V ++   ++T+V F LDDGTGRIDC +WV+E  D+ E+E + 
Sbjct: 61  SGLVINGISLTNVSLVGLVCDKDESKVTEVRFTLDDGTGRIDCKRWVSETFDAREMESVR 120

Query: 121 DGMYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSTT 180
           DG YVR+ GHLK+FQGK  L VFS+RP+ D+NE+T H+IECI+ +  N+  ++QQ    T
Sbjct: 121 DGTYVRLSGHLKTFQGKTQLLVFSVRPIMDFNEVTFHYIECIHFYSQNSESQRQQVGDVT 180

Query: 181 --TQPQMPNASNTPMKGYQAPIPNQYSGQAGGDGWKSVEQMVLDFLQLPSCD-NERGAHR 240
                     SNT       P+ +  +    G+G K+++ M+LD+L+ P+C   ++G H 
Sbjct: 181 QSVNTTFQGGSNTNQATLLNPVVSSQNND--GNGRKNLDDMILDYLKQPACTARQQGIHI 240

Query: 241 DVIAQQLKVPLEKLLPAMKNLEEEGLIYSTTDDFHFK 274
           D IAQQLK+P  KL   +++LE +GLIYST D++HFK
Sbjct: 241 DEIAQQLKIPKNKLEGVVQSLEGDGLIYSTIDEYHFK 275

[Close]

BLAST of Spg022823 vs. ExPASy Swiss-Prot
Match: Q6H7J5 (Replication protein A 32 kDa subunit B OS=Oryza sativa subsp. japonica OX=39947 GN=RPA2B PE=1 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 6.9e-52
Identity = 110/288 (38.19%), Postives = 176/288 (61.11%), Query Frame = 0


Query: 4   GSQFDG-----NAAFSGGGFMPSQTTQAPDHSFSPA----KNRDVQALLPLTVKQINNAF 63
           G  +DG     ++ F GGGFMPSQ T A + +        K+R+ QALLPLTVKQI +A 
Sbjct: 11  GGNYDGGGGNASSLFGGGGFMPSQATNAAEGTSGGGGGFPKSRNAQALLPLTVKQIMDAS 70

Query: 64  LSSDDKSNFVIDGVDVNNVKLIGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNE 123
            ++DDKSNF ++G++V+ V+L+G + N+  R+TDV+F LDDGTGR+  ++W N++ D+ E
Sbjct: 71  QTNDDKSNFAVNGMEVSTVRLVGRMLNKLDRVTDVSFTLDDGTGRVPVNRWENDSTDTKE 130

Query: 124 VEGILDGMYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQ 183
           +  I +G YV V+G LK FQGKR +  +S+R +T++N++T HF+ C++VH   TR + Q 
Sbjct: 131 MADIQNGDYVIVNGGLKGFQGKRQVVAYSVRRITNFNDVTHHFLHCVHVHLELTRPKSQV 190

Query: 184 SSSTTTQPQMPNASNTPMKGYQAPIPNQYS----GQAGGDGWKSVEQMVLDFLQLPSCDN 243
           +++T T           M   Q+P+ NQ S     Q  G G   ++ ++  F      ++
Sbjct: 191 NANTATGTPNQTMPRDSMAYNQSPLTNQASTFSAPQNTGTGTNMIDLVLNVFHDPAVMND 250

Query: 244 ERGAHRDVIAQQLKVPLEKLLPAMKNLEEEGLIYSTTDDFHFKSTANG 279
           + G   D ++++L +P E +   + +  + G +Y+T DD H+KST NG
Sbjct: 251 DHGVGVDYVSRRLNLPEETVGKIIIDQVDLGHLYATIDDHHYKSTMNG 298

[Close]

BLAST of Spg022823 vs. ExPASy Swiss-Prot
Match: Q6K9U2 (Replication protein A 32 kDa subunit A OS=Oryza sativa subsp. japonica OX=39947 GN=RPA2A PE=1 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 3.1e-44
Identity = 112/290 (38.62%), Postives = 165/290 (56.90%), Query Frame = 0


Query: 1   MMYGSQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINNA---FLSS 60
           MM  SQ D   AFS   F  SQ   A   S +P+K+R   + +PLTVKQI+ A    ++ 
Sbjct: 1   MMSFSQPD---AFSPSQFTSSQNAAA--DSTTPSKSRGASSTMPLTVKQISEAQQSGITG 60

Query: 61  DDKSNFVIDGVDVNNVKLIGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEG 120
           +  + FV+DGV+  NV+L+G+V  +  R TDV+F +DDGTGR+D  +WVN+ ADS E   
Sbjct: 61  EKGAPFVVDGVETANVRLVGLVSGKTERNTDVSFTIDDGTGRLDFIRWVNDGADSAETAA 120

Query: 121 ILDGMYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSS 180
           + +GMYV V G LK  Q ++    F+IRPVTDYNE+T HFI+C+ +H  NT  + Q  S 
Sbjct: 121 VQNGMYVSVIGSLKGLQERKRATAFAIRPVTDYNEVTLHFIQCVRMHLENT--KSQIGSP 180

Query: 181 TTTQPQMPNASN---------TPMKGYQAPIPNQYSGQAGGDGWKSVEQMVLDFLQLP-S 240
             T   M ++S+         T +K   AP+ +  +G         +   VL+  + P +
Sbjct: 181 AKTYSAMGSSSSNGFSEMTTPTSVKSNPAPVLSVTNGSK-----TDLNTEVLNVFREPAN 240

Query: 241 CDNERGAHRDVIAQQLKVPLEKLLPAMKNLEEEGLIYSTTDDFHFKSTAN 278
            ++E G H D I ++ ++P  K+  A+  L + G IYST D+ H+KS  N
Sbjct: 241 VESEHGVHIDEIVKRFRLPEAKIKVAIDYLADIGHIYSTIDESHYKSAFN 278

[Close]

BLAST of Spg022823 vs. ExPASy Swiss-Prot
Match: Q5Z8L1 (Replication protein A 32 kDa subunit C OS=Oryza sativa subsp. japonica OX=39947 GN=RPA2C PE=1 SV=2)

HSP 1 Score: 114.4 bits (285), Expect = 2.1e-24
Identity = 71/204 (34.80%), Postives = 111/204 (54.41%), Query Frame = 0


Query: 13  FSGGGFMPSQTTQAPDHSF-----------SPAKNRD--VQALLPLTVKQINNAF---LS 72
           FSG   MPSQ + AP   +           SP+K RD      +P TV  I+ +F   L+
Sbjct: 8   FSGTALMPSQRSGAPAPEYSAAGTGAAAAPSPSKPRDPRFSGCVPATVLHISRSFAAALA 67

Query: 73  SDDKSN--FVIDGVDVNNVKLIGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNE 132
           +D   +  F IDGV+  NV+++G V +   R TDV F LDD TG+I   +W+ + +D+ +
Sbjct: 68  ADGGGDPVFSIDGVETTNVRVLGRVVSVVSRDTDVCFTLDDSTGKIPLVRWITDQSDTRD 127

Query: 133 VEGILDGMYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIECIYVHF--YNTRLRK 192
              I +G+YV+V  +L  FQ K+     SIRP+ ++NE+  HFIEC++VH     +++++
Sbjct: 128 TSYIQEGVYVKVQVNLMGFQAKKQGLARSIRPINNFNEVVLHFIECMHVHLESVQSKMQR 187

Query: 193 QQSSSTTTQPQMPNASNTPMKGYQ 197
           Q   S  T       S+  ++ YQ
Sbjct: 188 QLPPSVQTNEYTHVPSSGGVRDYQ 211

[Close]

BLAST of Spg022823 vs. ExPASy TrEMBL
Match: A0A1S4DZE3 (replication protein A 32 kDa subunit B-like OS=Cucumis melo OX=3656 GN=LOC103494262 PE=3 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 5.3e-148
Identity = 261/277 (94.22%), Postives = 270/277 (97.47%), Query Frame = 0


Query: 2   MYGSQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINNAFLSSDDKS 61
           MY SQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQIN+AFLSSDDKS
Sbjct: 1   MYASQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINDAFLSSDDKS 60

Query: 62  NFVIDGVDVNNVKLIGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 121
           NFVIDGVDVNNVKL+GMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG
Sbjct: 61  NFVIDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 120

Query: 122 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSTTTQ 181
           MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEIT+HFIE IYVHFYNTRLRKQQSSS TTQ
Sbjct: 121 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQ 180

Query: 182 PQMPNASNTPMKGYQAPIPNQYSGQAGGDGWKSVEQMVLDFLQLPSCDNERGAHRDVIAQ 241
           PQM N SNTP+KGYQAPI NQY+GQAGGD WKS+EQMVLDFLQLPSCD+ERGAHRDVIAQ
Sbjct: 181 PQMTNLSNTPVKGYQAPIANQYTGQAGGDSWKSLEQMVLDFLQLPSCDSERGAHRDVIAQ 240

Query: 242 QLKVPLEKLLPAMKNLEEEGLIYSTTDDFHFKSTANG 279
           QLKVPLEKL+PAMKNLEEEGLIYSTTDD+HFKSTANG
Sbjct: 241 QLKVPLEKLIPAMKNLEEEGLIYSTTDDYHFKSTANG 277

[Close]

BLAST of Spg022823 vs. ExPASy TrEMBL
Match: A0A5D3CIS3 (Replication protein A 32 kDa subunit B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold152G00360 PE=3 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 5.3e-148
Identity = 261/277 (94.22%), Postives = 270/277 (97.47%), Query Frame = 0


Query: 2   MYGSQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINNAFLSSDDKS 61
           MY SQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQIN+AFLSSDDKS
Sbjct: 1   MYASQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINDAFLSSDDKS 60

Query: 62  NFVIDGVDVNNVKLIGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 121
           NFVIDGVDVNNVKL+GMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG
Sbjct: 61  NFVIDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 120

Query: 122 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSTTTQ 181
           MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEIT+HFIE IYVHFYNTRLRKQQSSS TTQ
Sbjct: 121 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITNHFIESIYVHFYNTRLRKQQSSSMTTQ 180

Query: 182 PQMPNASNTPMKGYQAPIPNQYSGQAGGDGWKSVEQMVLDFLQLPSCDNERGAHRDVIAQ 241
           PQM N SNTP+KGYQAPI NQY+GQAGGD WKS+EQMVLDFLQLPSCD+ERGAHRDVIAQ
Sbjct: 181 PQMTNLSNTPVKGYQAPIANQYTGQAGGDSWKSLEQMVLDFLQLPSCDSERGAHRDVIAQ 240

Query: 242 QLKVPLEKLLPAMKNLEEEGLIYSTTDDFHFKSTANG 279
           QLKVPLEKL+PAMKNLEEEGLIYSTTDD+HFKSTANG
Sbjct: 241 QLKVPLEKLIPAMKNLEEEGLIYSTTDDYHFKSTANG 277

[Close]

BLAST of Spg022823 vs. ExPASy TrEMBL
Match: A0A6J1JJ51 (replication protein A 32 kDa subunit B OS=Cucurbita maxima OX=3661 GN=LOC111485578 PE=3 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 1.9e-145
Identity = 255/277 (92.06%), Postives = 266/277 (96.03%), Query Frame = 0


Query: 2   MYGSQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINNAFLSSDDKS 61
           MYGSQFDGNAAFSGGGFMPSQTT APDHS SPAKNRDVQALLPLTVKQIN+AFLSSDDKS
Sbjct: 1   MYGSQFDGNAAFSGGGFMPSQTTMAPDHSLSPAKNRDVQALLPLTVKQINDAFLSSDDKS 60

Query: 62  NFVIDGVDVNNVKLIGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 121
           NFV+DGVDVNNVKL+GMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG
Sbjct: 61  NFVVDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 120

Query: 122 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSTTTQ 181
           MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIE IYVHFYNTRLRKQQSSS T Q
Sbjct: 121 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIESIYVHFYNTRLRKQQSSSMTPQ 180

Query: 182 PQMPNASNTPMKGYQAPIPNQYSGQAGGDGWKSVEQMVLDFLQLPSCDNERGAHRDVIAQ 241
           PQM N SNTP++GYQ P+ NQY+GQAGGDGWK++EQMVLDFLQLPSCD ERGAHRDVIAQ
Sbjct: 181 PQMANLSNTPVRGYQTPMSNQYTGQAGGDGWKNLEQMVLDFLQLPSCDTERGAHRDVIAQ 240

Query: 242 QLKVPLEKLLPAMKNLEEEGLIYSTTDDFHFKSTANG 279
            LKVPLEKL+PAMKNLEEEGLIYSTTDD+HFKSTANG
Sbjct: 241 HLKVPLEKLMPAMKNLEEEGLIYSTTDDYHFKSTANG 277

[Close]

BLAST of Spg022823 vs. ExPASy TrEMBL
Match: A0A6J1E347 (replication protein A 32 kDa subunit B-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430133 PE=3 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 9.4e-145
Identity = 254/277 (91.70%), Postives = 265/277 (95.67%), Query Frame = 0


Query: 2   MYGSQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINNAFLSSDDKS 61
           MYGSQFDGNAAFSGGGFMPSQTT APDHS SPAKNRDVQALLPLTVKQIN+AFLSSDDKS
Sbjct: 1   MYGSQFDGNAAFSGGGFMPSQTTMAPDHSLSPAKNRDVQALLPLTVKQINDAFLSSDDKS 60

Query: 62  NFVIDGVDVNNVKLIGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 121
           NFV+DGVDVNNVKL+GMVRNRAGRITDVTFALDDGTG+IDCSKWVNEAADSNEVEGILDG
Sbjct: 61  NFVVDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGQIDCSKWVNEAADSNEVEGILDG 120

Query: 122 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSTTTQ 181
           MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIE IYVHFYNTRLRKQQSSS T Q
Sbjct: 121 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIESIYVHFYNTRLRKQQSSSMTPQ 180

Query: 182 PQMPNASNTPMKGYQAPIPNQYSGQAGGDGWKSVEQMVLDFLQLPSCDNERGAHRDVIAQ 241
           PQM N SNTP++GYQ PI NQY+GQAGGDGWK++EQMVLDFLQLPSCD ERGAHRDVIAQ
Sbjct: 181 PQMANLSNTPVRGYQTPISNQYTGQAGGDGWKNLEQMVLDFLQLPSCDTERGAHRDVIAQ 240

Query: 242 QLKVPLEKLLPAMKNLEEEGLIYSTTDDFHFKSTANG 279
            L VPLEKL+PAMKNLEEEGLIYSTTDD+HFKSTANG
Sbjct: 241 HLNVPLEKLMPAMKNLEEEGLIYSTTDDYHFKSTANG 277

[Close]

BLAST of Spg022823 vs. ExPASy TrEMBL
Match: A0A6J1E235 (replication protein A 32 kDa subunit B-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430133 PE=3 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 8.8e-143
Identity = 253/277 (91.34%), Postives = 264/277 (95.31%), Query Frame = 0


Query: 2   MYGSQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINNAFLSSDDKS 61
           MYGSQFDGNAAFSGGGFMPSQTT APDHS SPAKNRDVQALLPLTVKQIN+AFLSSDDKS
Sbjct: 1   MYGSQFDGNAAFSGGGFMPSQTTMAPDHSLSPAKNRDVQALLPLTVKQINDAFLSSDDKS 60

Query: 62  NFVIDGVDVNNVKLIGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 121
           NFV+DGVDVNNVKL+GMVRNRAGRITDVTFALDDGTG+IDCSKWVNEAADSNEVEGILDG
Sbjct: 61  NFVVDGVDVNNVKLVGMVRNRAGRITDVTFALDDGTGQIDCSKWVNEAADSNEVEGILDG 120

Query: 122 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSTTTQ 181
           MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIE IYVHFYNTRLR QQSSS T Q
Sbjct: 121 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIESIYVHFYNTRLR-QQSSSMTPQ 180

Query: 182 PQMPNASNTPMKGYQAPIPNQYSGQAGGDGWKSVEQMVLDFLQLPSCDNERGAHRDVIAQ 241
           PQM N SNTP++GYQ PI NQY+GQAGGDGWK++EQMVLDFLQLPSCD ERGAHRDVIAQ
Sbjct: 181 PQMANLSNTPVRGYQTPISNQYTGQAGGDGWKNLEQMVLDFLQLPSCDTERGAHRDVIAQ 240

Query: 242 QLKVPLEKLLPAMKNLEEEGLIYSTTDDFHFKSTANG 279
            L VPLEKL+PAMKNLEEEGLIYSTTDD+HFKSTANG
Sbjct: 241 HLNVPLEKLMPAMKNLEEEGLIYSTTDDYHFKSTANG 276

[Close]

BLAST of Spg022823 vs. TAIR 10
Match: AT3G02920.1 (Replication protein A, subunit RPA32 )

HSP 1 Score: 272.3 bits (695), Expect = 4.2e-73
Identity = 142/280 (50.71%), Postives = 188/280 (67.14%), Query Frame = 0


Query: 2   MYGSQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINNAFLSSDDKS 61
           MYG  FDGNAAF+GGGFMPSQ T     S S  KNRDV+ LLPLT+KQ+++A  S+  +S
Sbjct: 1   MYGGDFDGNAAFAGGGFMPSQATTQAHESSSSLKNRDVRTLLPLTLKQLSSA--STTGES 60

Query: 62  NFVIDGVDVNNVKLIGMVRNRAGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGILDG 121
           NF IDGVD+  V ++G +     RIT V F +DDGTG +DC +W +   ++ E+E +  G
Sbjct: 61  NFSIDGVDIKTVVIVGRISRMENRITQVDFVVDDGTGWVDCVRWCHARQETEEMEAVKLG 120

Query: 122 MYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLR--KQQSSSTT 181
           MYVR+HGHLK FQGKR++NVFS+RPVTD+NEI  HF EC+YVH YNT+LR       + T
Sbjct: 121 MYVRLHGHLKIFQGKRSVNVFSVRPVTDFNEIVHHFTECMYVHMYNTKLRGGSITQDTAT 180

Query: 182 TQPQMP-NASNTPMKGYQAPIPNQYSGQAGGDGWKSVEQMVLDFLQLP-SCDNERGAHRD 241
            +PQMP +   TP K YQ    NQ+  Q   D    V+Q VL++L  P    +E G H D
Sbjct: 181 PRPQMPYSTMPTPAKPYQTGPSNQFPNQF-NDSMHGVKQTVLNYLNQPMHIVSEAGVHCD 240

Query: 242 VIAQQLKVPLEKLLPAMKNLEEEGLIYSTTDDFHFKSTAN 278
           +IA++L++PL ++  A++ L  +G IYST D+  FKSTAN
Sbjct: 241 IIARELRIPLLQVKEALEQLSNDGCIYSTLDETCFKSTAN 277

[Close]

BLAST of Spg022823 vs. TAIR 10
Match: AT3G02920.2 (Replication protein A, subunit RPA32 )

HSP 1 Score: 261.5 bits (667), Expect = 7.5e-70
Identity = 144/300 (48.00%), Postives = 192/300 (64.00%), Query Frame = 0


Query: 2   MYGSQFDGNAAFSGGGFMPSQ-TTQAPDHSF-------------------SPAKNRDVQA 61
           MYG  FDGNAAF+GGGFMPSQ TTQA + S                    S  +NRDV+ 
Sbjct: 1   MYGGDFDGNAAFAGGGFMPSQATTQAHESSSSLKVYVSDSRRFSGISAAKSSIRNRDVRT 60

Query: 62  LLPLTVKQINNAFLSSDDKSNFVIDGVDVNNVKLIGMVRNRAGRITDVTFALDDGTGRID 121
           LLPLT+KQ+++A  S+  +SNF IDGVD+  V ++G +     RIT V F +DDGTG +D
Sbjct: 61  LLPLTLKQLSSA--STTGESNFSIDGVDIKTVVIVGRISRMENRITQVDFVVDDGTGWVD 120

Query: 122 CSKWVNEAADSNEVEGILDGMYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIECI 181
           C +W +   ++ E+E +  GMYVR+HGHLK FQGKR++NVFS+RPVTD+NEI  HF EC+
Sbjct: 121 CVRWCHARQETEEMEAVKLGMYVRLHGHLKIFQGKRSVNVFSVRPVTDFNEIVHHFTECM 180

Query: 182 YVHFYNTRLR--KQQSSSTTTQPQMP-NASNTPMKGYQAPIPNQYSGQAGGDGWKSVEQM 241
           YVH YNT+LR       + T +PQMP +   TP K YQ    NQ+  Q   D    V+Q 
Sbjct: 181 YVHMYNTKLRGGSITQDTATPRPQMPYSTMPTPAKPYQTGPSNQFPNQF-NDSMHGVKQT 240

Query: 242 VLDFLQLP-SCDNERGAHRDVIAQQLKVPLEKLLPAMKNLEEEGLIYSTTDDFHFKSTAN 278
           VL++L  P    +E G H D+IA++L++PL ++  A++ L  +G IYST D+  FKSTAN
Sbjct: 241 VLNYLNQPMHIVSEAGVHCDIIARELRIPLLQVKEALEQLSNDGCIYSTLDETCFKSTAN 297

[Close]

BLAST of Spg022823 vs. TAIR 10
Match: AT2G24490.1 (replicon protein A2 )

HSP 1 Score: 259.2 bits (661), Expect = 3.7e-69
Identity = 132/277 (47.65%), Postives = 186/277 (67.15%), Query Frame = 0


Query: 1   MMYGSQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINNAFLSSDDK 60
           M   SQF+ N+ FSGGGFM SQ +QA + S S AKNRD Q L+P+TVKQI   F SS +K
Sbjct: 1   MFSSSQFEPNSGFSGGGFMSSQPSQAYESSSSTAKNRDFQGLVPVTVKQITECFQSSGEK 60

Query: 61  SNFVIDGVDVNNVKLIGMVRNR-AGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGIL 120
           S  VI+G+ + NV L+G+V ++   ++T+V F LDDGTGRIDC +WV+E  D+ E+E + 
Sbjct: 61  SGLVINGISLTNVSLVGLVCDKDESKVTEVRFTLDDGTGRIDCKRWVSETFDAREMESVR 120

Query: 121 DGMYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSTT 180
           DG YVR+ GHLK+FQGK  L VFS+RP+ D+NE+T H+IECI+ +  N+  ++QQ    T
Sbjct: 121 DGTYVRLSGHLKTFQGKTQLLVFSVRPIMDFNEVTFHYIECIHFYSQNSESQRQQVGDVT 180

Query: 181 --TQPQMPNASNTPMKGYQAPIPNQYSGQAGGDGWKSVEQMVLDFLQLPSCD-NERGAHR 240
                     SNT       P+ +  +    G+G K+++ M+LD+L+ P+C   ++G H 
Sbjct: 181 QSVNTTFQGGSNTNQATLLNPVVSSQNND--GNGRKNLDDMILDYLKQPACTARQQGIHI 240

Query: 241 DVIAQQLKVPLEKLLPAMKNLEEEGLIYSTTDDFHFK 274
           D IAQQLK+P  KL   +++LE +GLIYST D++HFK
Sbjct: 241 DEIAQQLKIPKNKLEGVVQSLEGDGLIYSTIDEYHFK 275

[Close]

BLAST of Spg022823 vs. TAIR 10
Match: AT2G24490.2 (replicon protein A2 )

HSP 1 Score: 259.2 bits (661), Expect = 3.7e-69
Identity = 132/277 (47.65%), Postives = 186/277 (67.15%), Query Frame = 0


Query: 1   MMYGSQFDGNAAFSGGGFMPSQTTQAPDHSFSPAKNRDVQALLPLTVKQINNAFLSSDDK 60
           M   SQF+ N+ FSGGGFM SQ +QA + S S AKNRD Q L+P+TVKQI   F SS +K
Sbjct: 1   MFSSSQFEPNSGFSGGGFMSSQPSQAYESSSSTAKNRDFQGLVPVTVKQITECFQSSGEK 60

Query: 61  SNFVIDGVDVNNVKLIGMVRNR-AGRITDVTFALDDGTGRIDCSKWVNEAADSNEVEGIL 120
           S  VI+G+ + NV L+G+V ++   ++T+V F LDDGTGRIDC +WV+E  D+ E+E + 
Sbjct: 61  SGLVINGISLTNVSLVGLVCDKDESKVTEVRFTLDDGTGRIDCKRWVSETFDAREMESVR 120

Query: 121 DGMYVRVHGHLKSFQGKRTLNVFSIRPVTDYNEITSHFIECIYVHFYNTRLRKQQSSSTT 180
           DG YVR+ GHLK+FQGK  L VFS+RP+ D+NE+T H+IECI+ +  N+  ++QQ    T
Sbjct: 121 DGTYVRLSGHLKTFQGKTQLLVFSVRPIMDFNEVTFHYIECIHFYSQNSESQRQQVGDVT 180

Query: 181 --TQPQMPNASNTPMKGYQAPIPNQYSGQAGGDGWKSVEQMVLDFLQLPSCD-NERGAHR 240
                     SNT       P+ +  +    G+G K+++ M+LD+L+ P+C   ++G H 
Sbjct: 181 QSVNTTFQGGSNTNQATLLNPVVSSQNND--GNGRKNLDDMILDYLKQPACTARQQGIHI 240

Query: 241 DVIAQQLKVPLEKLLPAMKNLEEEGLIYSTTDDFHFK 274
           D IAQQLK+P  KL   +++LE +GLIYST D++HFK
Sbjct: 241 DEIAQQLKIPKNKLEGVVQSLEGDGLIYSTIDEYHFK 275

[Close]

BLAST of Spg022823 vs. TAIR 10
Match: AT1G07130.1 (Nucleic acid-binding, OB-fold-like protein )

HSP 1 Score: 44.7 bits (104), Expect = 1.4e-04
Identity = 31/136 (22.79%), Postives = 56/136 (41.18%), Query Frame = 0


Query: 45  LTVKQINNAFLSSDDKSNF-VIDGVDVNNVKLIGMVRNRAGRITDVTFALDDGTGRIDCS 104
           L  + I     S  + ++F ++ G  V+ V+++G + +R      + F +DDGTG + C 
Sbjct: 12  LVARDIQRLTQSPTESNSFSLLGGACVSRVEIVGTIVSRDLTPKFLKFGVDDGTGCVTCV 71

Query: 105 KWVNEAADS------------------NEVEGILDGMYVRVHGHLKSFQGKRTLNVFSIR 162
            W+N+   S                   +   I  G   RV G + S++G   +      
Sbjct: 72  MWLNQLTSSYFSRWDPATILLLASAARKQAAQIRIGAVARVRGRVGSYRGVMQITANVAV 131

The following BLAST results are available for this feature:

BLAST of Spg022823 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008453602.1	1.1e-147	94.22	PREDICTED: replication protein A 32 kDa subunit B-like [Cucumis melo] >XP_008453...	[more]
XP_011656845.1	1.9e-147	94.58	replication protein A 32 kDa subunit B [Cucumis sativus] >KAE8653166.1 hypotheti...	[more]
XP_038880298.1	2.7e-146	94.22	replication protein A 32 kDa subunit B [Benincasa hispida]	[more]
XP_023516911.1	7.9e-146	92.78	replication protein A 32 kDa subunit B-like isoform X1 [Cucurbita pepo subsp. pe...	[more]
XP_022988280.1	3.9e-145	92.06	replication protein A 32 kDa subunit B [Cucurbita maxima]	[more]

BLAST of Spg022823 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LFJ8	6.0e-72	50.71	Replication protein A 32 kDa subunit B OS=Arabidopsis thaliana OX=3702 GN=RPA2B ...	[more]
Q9ZQ19	5.2e-68	47.65	Replication protein A 32 kDa subunit A OS=Arabidopsis thaliana OX=3702 GN=RPA2A ...	[more]
Q6H7J5	6.9e-52	38.19	Replication protein A 32 kDa subunit B OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q6K9U2	3.1e-44	38.62	Replication protein A 32 kDa subunit A OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q5Z8L1	2.1e-24	34.80	Replication protein A 32 kDa subunit C OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of Spg022823 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S4DZE3	5.3e-148	94.22	replication protein A 32 kDa subunit B-like OS=Cucumis melo OX=3656 GN=LOC103494...	[more]
A0A5D3CIS3	5.3e-148	94.22	Replication protein A 32 kDa subunit B-like OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A6J1JJ51	1.9e-145	92.06	replication protein A 32 kDa subunit B OS=Cucurbita maxima OX=3661 GN=LOC1114855...	[more]
A0A6J1E347	9.4e-145	91.70	replication protein A 32 kDa subunit B-like isoform X1 OS=Cucurbita moschata OX=...	[more]
A0A6J1E235	8.8e-143	91.34	replication protein A 32 kDa subunit B-like isoform X2 OS=Cucurbita moschata OX=...	[more]

BLAST of Spg022823 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G02920.1	4.2e-73	50.71	Replication protein A, subunit RPA32	[more]
AT3G02920.2	7.5e-70	48.00	Replication protein A, subunit RPA32	[more]
AT2G24490.1	3.7e-69	47.65	replicon protein A2	[more]
AT2G24490.2	3.7e-69	47.65	replicon protein A2	[more]
AT1G07130.1	1.4e-04	22.79	Nucleic acid-binding, OB-fold-like protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	2.40.50.140		coord: 38..170 e-value: 5.4E-42 score: 144.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 16..31
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 173..203
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 14..33
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 173..207
None	No IPR available	PANTHER	PTHR13989:SF42	REPLICATION PROTEIN A 32 KDA SUBUNIT A-LIKE	coord: 4..278
None	No IPR available	CDD	cd04478	RPA2_DBD_D	coord: 72..165 e-value: 5.93477E-42 score: 137.731
IPR004365	OB-fold nucleic acid binding domain, AA-tRNA synthetase-type	PFAM	PF01336	tRNA_anti-codon	coord: 73..146 e-value: 1.4E-8 score: 34.6
IPR014646	Replication factor A protein 2	PIRSF	PIRSF036949	RPA32	coord: 2..276 e-value: 1.6E-79 score: 264.4
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 207..277 e-value: 7.0E-17 score: 62.9
IPR014892	Replication protein A, C-terminal	PFAM	PF08784	RPA_C	coord: 164..269 e-value: 5.5E-16 score: 59.1
IPR040260	Replication factor A protein-like	PANTHER	PTHR13989	REPLICATION PROTEIN A-RELATED	coord: 4..278
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 210..276
IPR012340	Nucleic acid-binding, OB-fold	SUPERFAMILY	50249	Nucleic acid-binding proteins	coord: 42..169

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg022823.1	Spg022823.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006260	DNA replication
biological_process	GO:0000724	double-strand break repair via homologous recombination
biological_process	GO:0006289	nucleotide-excision repair
biological_process	GO:0006310	DNA recombination
biological_process	GO:0006281	DNA repair
cellular_component	GO:0000781	chromosome, telomeric region
cellular_component	GO:0005662	DNA replication factor A complex
cellular_component	GO:0035861	site of double-strand break
cellular_component	GO:0005634	nucleus
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0003697	single-stranded DNA binding
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003676	nucleic acid binding