Spg022760 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg022760
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionCholine monooxygenase, chloroplastic
Locationscaffold2: 2662989 .. 2666885 (-)
RNA-Seq ExpressionSpg022760
SyntenySpg022760
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTACAGAGAAGCTTGGTGATTGTTGCAATGGCGACGTTAACGAAGCAAATCCCTACTCACTTCTTCCAAGCTCCTCGTCCTTGGATCACTTGCCCTTCATCTTCCTCACGAATCCCGGCGGCTTTCTCCTTTCGAAAACACGATTCTTACTCCATTGAAGCTCAGAAACTCGTCGATGAATTCGACCCTAAAATTCCCTTGGAGAAGGCCGTCACTCCACCCAGCTCGTGGTACACCGATCCTTCGTTTTTCGCTCTCGAGCTCGATCGTGTCTTCTGCAGAGGATGGCAGGCCGTAGGTTCGCTTCCATCTCTACATTGTTGTTGTTCTTCTTCTTTTGCGGCTTTATTTGATTGTATATGCAAAGCGAGGAGATTAATTGTTAAATGTTAATGTTACTGCAATTTGTGACCTATGACTATAATGGAAGATTGTTTAGACATTAGAGTTCCCAGCCAAGTCTTTTTTTGTGGATTTTAGGCTTTCGTAAACCAATTTTTAGTATTTCCTTGTTGTGACATCACGCTAGAAAGAGAATTCAATCTTTACTGAATATCTACCTTTTGAACTTGCAAAATAGAAAAAAGAACAGGAGAATGGCCATGGCTCGATTACTGCCCTTTTTGTGGTGATTTTTGTGCTTGACATATTTACATGTTCTTCCATTTAGTGTTAAGAGTTACTCAACCAGACCTCGAGTGTGAGAGAGGATGAAGGAAAGTAACCTGTGGCGAATAGTTTCTGCCGTGCATGATCTGAGAGCTTAGAAGTTTTTGCTGTTTGACAGAGTGATTCTTTATGGGGTTTCTCATGCACTCAAAGTTGGCCTATGCCATTGAAATTGCGTCCTCTTCTCTGTTCCTTTTTTTATTTTATCTTCTTTCCCTGTAGATTATAACTCTGACTATACTAATGCATGGATCATCAACTTCTTGTGCCATTGACTTGCCAGGAAATGTTGAACAGTTAAAAGATCCCCATGACTTTTTCACAGGCAGGTGTGATTAACTGTCAGTTACCACTTACTACTGATAGTATAAAATCCAAAGAGCAGAAATAAGGGTGTGTTTGGATTACCTTTGTAAGTGGTTATGAAGGATCAAAATGTTTTTTTTAAGAAAATATAGTGTTTGGCAACAAAAAGTGATAATTACTTTGAAATAATTTGATTGGTTAATAATCGTTTTATCAATAATCAATTTTAAATGACTATCAAACAAAGTGATTTCTGTTCAATCATTTTAGAATCATTCTAAAAAAAACTCTTCACAGCAAAGAATTAAAAAAAAAAAAAAAATCCCAAAGAAGTAGTTAAAAACACTTTCATATTAAAGAAATCCAAAATCACTCAAAAACCCAAAGAATCACTTAAAAAAAAAAACTTTTCACTTTAGAATCACCTAAAAAGCTCTTAATTCAAGAATTCCACAATCCACATGAAAAAAACCTGAAAAATGCGGAATTACAACATTGCCCTCCAATTTAAAGTTTTTTCTTTTCTCATTATAGCAATTTAACATCTGTAGAATCATTTTCTACCACCAAACAAAGTTATGATTAAAATAATTTTTAAGAGGTAACCAAACACACAAATCTTTAAATCAAAATCACTTTAATGAAAAGTGTTTCAAGATAAAACAAGAAAAAGTGTTTAAATGAGCATATATGCCAAACTCACCCTGAAAAGTGAAACTATTTCAGATGCCATAATTAAACCAAATATGGAGGTGAGTCTGTTGACATTTTTAACACCTCGAGTCTAATATATGGAATGCTAGAGACGAGTGTTTCTCCTCACCATAGCTTGTCCATGAAATTTGTTGACTCTTTGCTCTTTGACCATGGCAGGTTGGGAAATGTAGAGTTTGTGGTGTGCAAAGATAATAACAGGAAGGTTCGTGCATTTCACAATGTCTGCCGCCATCATGCTTCACTTATTGCTTCTGGAAGTGGGAAAAAGTCGTGCTTTGTATGCCCGTATCATGTAAGTTCACTTCATTTCTTTTTGTAGGTTCCTTGGGAATTTTTTGGTTTTCTTTCTGGTTATTTATTGCTGAGCTTATTAAGGTTTTAGTCCACAACTTTAACTTCCTATTGAAAGGAAACGGTAATATCCATTGAAGAAAAAAGAGATCATTGTTAAAGGTTGAAATAATATCATCCAATCCAACCATATCAAGTCAATGCATTGGAAATCTCCAAGCTCTGCTCTGATCTTATGCAAAAATTTTAAAATGCAATTAGGATGTAAGATTCTGAGATATTACAAGGTGCGTCTATATGTATTGAAGAGCCTTATTCCGTATTTTCTACAGGGATGGACTTATGGGTTGGATGGAGTTCTGCTTAAGGCGACTAGAATAAATGGGATACAAAACTTTGATGTAAATGTATGTGATTATATCATAGTGATACCAGTTCCTAAAACCATTTTAATATAATATTTCTGAATGGCTTAGTAATTCTAATTGGTTAATTTTTTTAATGATGGAGATTAGGATTTTGGGCTCATACCAATACCGGCAGCTACATGGGGGCCTTTCATTCTTCTCAATCTGGATAAAAAATTATCATCTGAGCTGGATGTTGATGAAGATAAAGTAGCACATGAATGGCTTGGAAGCCGTGCAGATGTGCTGAGTTTGAATGGAGTTGATGCTTCTTTAAGTTTTGTTTGTCGACGTGAATACACCATTGAATGTAATTGGAAGGTATAGTCCTTGCATCCTTCTAGTTACCACACTTCGATCAAGAGTTCAAGTTTCTGAAAAGAAAAAAAAGAAAAAAAGAACAGGAAGAAGACGTATGACCAATAGTTCTGAGGACGAGTGCAGCCCATCAAAGGACGAGGAACATTTGTCTGTTGTAAAAATTATTTGGCATCTCTGAATTCGGAAATCAGCAATCAAAATCAGGGAACATTTTCTTCCATAATCCTTATGGTTTTGGTAATATGTTTTATTGATTTTTTTGCAGGTTTATTGTGACAACTACTTAGACGGAGGATATCACGTTCCCTATGCACATAAAGGGCTAGCATCAAATCTGAATATTGATTCGTATTCTACAGAAGTACGTTGACTGTTTATTTTAAATTTATTACCAACTGCTCTTATAGCATTATGCTTTCATGCAATTTGCTGTTTTAGGATTTGTTTACTTCATTATTTAAGTTGTTGTTCTGTTTTCACCTAAGGTCTATAAGACCATATAAAATAAAAATGCATGTTTATAGTAGTTAATCATTCGCTGTTAATTATCAATGTCATGTGACTATAACTTGTCATGTAGACGTTTGAAACTGTTAGCATTCAAAGTTGTAAGGCAAAAGGCGATGATTATGGTCGACTTGGATCAGAAGCACTATATGCTTTTATATACCCAAACTTCATGATAAATAGGTAGTTCCTGGCTACTTCAACATTGCTGGCTCTTTGGCTCTTTATCTTTTAGTTGTTCTGCCGTGATGTTTGTCTCATCAATTTCGAGTTCTATGACAGGTATGGACCTTGGATGGACACTAATCTAGTACTGCCACTTGGACCTCGAAAATGTCTTGTGGTTTTTGATTATTTTCTTGAAGCTCCTTTCAAGGTAATTTCGAGTATTTTGGCCTGATGGATGTGCAATAAGTCCTTTCTGAGTGTGCATGAGTTAACTCATATTCTTGATTTGTTAAAAGGACTGCTCAGTCGACAAACCAGTCCATATCTAATACGAAAACTCTGCAAAAACTGGGCATTTGTATTGTAAAGGATTGTCCGTTCATTCGGGTTTTTACCTTTCTCTTCCTTGATATTCAGAATGACGACTCTCTTATACAAAACAGTTTAGAAGACAGTGAAAGCGTGCAGGTATTACAAACAAAACAAATCCTCCCCTTTCCATCTCTATCAT

mRNA sequence

TTACAGAGAAGCTTGGTGATTGTTGCAATGGCGACGTTAACGAAGCAAATCCCTACTCACTTCTTCCAAGCTCCTCGTCCTTGGATCACTTGCCCTTCATCTTCCTCACGAATCCCGGCGGCTTTCTCCTTTCGAAAACACGATTCTTACTCCATTGAAGCTCAGAAACTCGTCGATGAATTCGACCCTAAAATTCCCTTGGAGAAGGCCGTCACTCCACCCAGCTCGTGGTACACCGATCCTTCGTTTTTCGCTCTCGAGCTCGATCGTGTCTTCTGCAGAGGATGGCAGGCCGTAGGTTCGCTTCCATCTCTACATTGTTGTTGTTCTTCTTCTTTTGCGGCTTTATTTGATTGTATATGCAAAGCGAGGAGATTAATTATTATAACTCTGACTATACTAATGCATGGATCATCAACTTCTTGTGCCATTGACTTGCCAGGAAATGTTGAACAGTTAAAAGATCCCCATGACTTTTTCACAGGCAGGTTGGGAAATGTAGAGTTTGTGGTGTGCAAAGATAATAACAGGAAGGTTCGTGCATTTCACAATGTCTGCCGCCATCATGCTTCACTTATTGCTTCTGGAAGTGGGAAAAAGTCGTGCTTTGTATGCCCGTATCATGGATGGACTTATGGGTTGGATGGAGTTCTGCTTAAGGCGACTAGAATAAATGGGATACAAAACTTTGATGTAAATGATTTTGGGCTCATACCAATACCGGCAGCTACATGGGGGCCTTTCATTCTTCTCAATCTGGATAAAAAATTATCATCTGAGCTGGATGTTGATGAAGATAAAGTAGCACATGAATGGCTTGGAAGCCGTGCAGATGTGCTGAGTTTGAATGGAGTTGATGCTTCTTTAAGTTTTGTTTGTCGACGTGAATACACCATTGAATGTAATTGGAAGGTTTATTGTGACAACTACTTAGACGGAGGATATCACGTTCCCTATGCACATAAAGGGCTAGCATCAAATCTGAATATTGATTCGTATTCTACAGAAGTACGTTGA

Coding sequence (CDS)

TTACAGAGAAGCTTGGTGATTGTTGCAATGGCGACGTTAACGAAGCAAATCCCTACTCACTTCTTCCAAGCTCCTCGTCCTTGGATCACTTGCCCTTCATCTTCCTCACGAATCCCGGCGGCTTTCTCCTTTCGAAAACACGATTCTTACTCCATTGAAGCTCAGAAACTCGTCGATGAATTCGACCCTAAAATTCCCTTGGAGAAGGCCGTCACTCCACCCAGCTCGTGGTACACCGATCCTTCGTTTTTCGCTCTCGAGCTCGATCGTGTCTTCTGCAGAGGATGGCAGGCCGTAGGTTCGCTTCCATCTCTACATTGTTGTTGTTCTTCTTCTTTTGCGGCTTTATTTGATTGTATATGCAAAGCGAGGAGATTAATTATTATAACTCTGACTATACTAATGCATGGATCATCAACTTCTTGTGCCATTGACTTGCCAGGAAATGTTGAACAGTTAAAAGATCCCCATGACTTTTTCACAGGCAGGTTGGGAAATGTAGAGTTTGTGGTGTGCAAAGATAATAACAGGAAGGTTCGTGCATTTCACAATGTCTGCCGCCATCATGCTTCACTTATTGCTTCTGGAAGTGGGAAAAAGTCGTGCTTTGTATGCCCGTATCATGGATGGACTTATGGGTTGGATGGAGTTCTGCTTAAGGCGACTAGAATAAATGGGATACAAAACTTTGATGTAAATGATTTTGGGCTCATACCAATACCGGCAGCTACATGGGGGCCTTTCATTCTTCTCAATCTGGATAAAAAATTATCATCTGAGCTGGATGTTGATGAAGATAAAGTAGCACATGAATGGCTTGGAAGCCGTGCAGATGTGCTGAGTTTGAATGGAGTTGATGCTTCTTTAAGTTTTGTTTGTCGACGTGAATACACCATTGAATGTAATTGGAAGGTTTATTGTGACAACTACTTAGACGGAGGATATCACGTTCCCTATGCACATAAAGGGCTAGCATCAAATCTGAATATTGATTCGTATTCTACAGAAGTACGTTGA

Protein sequence

LQRSLVIVAMATLTKQIPTHFFQAPRPWITCPSSSSRIPAAFSFRKHDSYSIEAQKLVDEFDPKIPLEKAVTPPSSWYTDPSFFALELDRVFCRGWQAVGSLPSLHCCCSSSFAALFDCICKARRLIIITLTILMHGSSTSCAIDLPGNVEQLKDPHDFFTGRLGNVEFVVCKDNNRKVRAFHNVCRHHASLIASGSGKKSCFVCPYHGWTYGLDGVLLKATRINGIQNFDVNDFGLIPIPAATWGPFILLNLDKKLSSELDVDEDKVAHEWLGSRADVLSLNGVDASLSFVCRREYTIECNWKVYCDNYLDGGYHVPYAHKGLASNLNIDSYSTEVR
Homology
BLAST of Spg022760 vs. NCBI nr
Match: XP_016899776.1 (PREDICTED: choline monooxygenase, chloroplastic isoform X1 [Cucumis melo])

HSP 1 Score: 496.1 bits (1276), Expect = 2.4e-136
Identity = 243/332 (73.19%), Postives = 265/332 (79.82%), Query Frame = 0

Query: 10  MATLTKQIPTHFFQAPR---PWITC-PSSSSRIPAAFSFRKHDSYSIEAQKLVDEFDPKI 69
           MATLTK I  HFFQ P     +  C   S SRI A+ SFR  DS  IEA+KLVD+FDP+I
Sbjct: 1   MATLTKHIQIHFFQPPSISFNFHFCNHRSPSRISASLSFRSPDSRFIEARKLVDDFDPEI 60

Query: 70  PLEKAVTPPSSWYTDPSFFALELDRVFCRGWQAVGSLPSLHCCCSSSFAALFDCICKARR 129
           PLEKA+TPPSSWY DPSFFALELDRVF RGWQA                           
Sbjct: 61  PLEKALTPPSSWYIDPSFFALELDRVFYRGWQA--------------------------- 120

Query: 130 LIIITLTILMHGSSTSCAIDLPGNVEQLKDPHDFFTGRLGNVEFVVCKDNNRKVRAFHNV 189
             IITLTIL HGSST CAI L G VEQLKD HDFFTGRLGNVE+VVCKDNNRKVRAFHNV
Sbjct: 121 --IITLTILRHGSSTFCAIGLSGYVEQLKDAHDFFTGRLGNVEYVVCKDNNRKVRAFHNV 180

Query: 190 CRHHASLIASGSGKKSCFVCPYHGWTYGLDGVLLKATRINGIQNFDVNDFGLIPIPAATW 249
           CRHHASL+A+G GKKSCFVCPYHGWTYGLDG+LLKATRINGIQNF+ NDFGL+P+P A W
Sbjct: 181 CRHHASLLATGCGKKSCFVCPYHGWTYGLDGILLKATRINGIQNFNENDFGLVPLPVAMW 240

Query: 250 GPFILLNLDKKLSSELDVDEDKVAHEWLGSRADVLSLNGVDASLSFVCRREYTIECNWKV 309
           GPF+LLNLD KLSS+ DVDEDKVA EWLG+ ADVL LNGVDASLS+VCRREYTI+CNWKV
Sbjct: 241 GPFVLLNLDGKLSSKPDVDEDKVAREWLGTCADVLRLNGVDASLSYVCRREYTIDCNWKV 300

Query: 310 YCDNYLDGGYHVPYAHKGLASNLNIDSYSTEV 338
           +CDNYLDGGYHVPYAHKGLASNLN++SYSTE+
Sbjct: 301 FCDNYLDGGYHVPYAHKGLASNLNLESYSTEL 303

BLAST of Spg022760 vs. NCBI nr
Match: XP_038880722.1 (choline monooxygenase, chloroplastic [Benincasa hispida])

HSP 1 Score: 483.4 bits (1243), Expect = 1.6e-132
Identity = 239/332 (71.99%), Postives = 253/332 (76.20%), Query Frame = 0

Query: 10  MATLTKQIPTHFFQAPRPWITCPS----SSSRIPAAFSFRKHDSYSIEAQKLVDEFDPKI 69
           MATLTK I THFFQ P       S    S SRI AA SFR  DS+ IEAQKLVDEFDP+I
Sbjct: 1   MATLTKHIHTHFFQPPSISFNLHSCNHRSPSRISAALSFRNSDSHFIEAQKLVDEFDPEI 60

Query: 70  PLEKAVTPPSSWYTDPSFFALELDRVFCRGWQAVGSLPSLHCCCSSSFAALFDCICKARR 129
           PLEKAVTPPSSWY DPSF+ALELDRVF RGWQAV                          
Sbjct: 61  PLEKAVTPPSSWYIDPSFYALELDRVFYRGWQAV-------------------------- 120

Query: 130 LIIITLTILMHGSSTSCAIDLPGNVEQLKDPHDFFTGRLGNVEFVVCKDNNRKVRAFHNV 189
                                 G VEQLKDPHDFFTGRLGNVE+VVCKDNNRKVRAFHNV
Sbjct: 121 ----------------------GYVEQLKDPHDFFTGRLGNVEYVVCKDNNRKVRAFHNV 180

Query: 190 CRHHASLIASGSGKKSCFVCPYHGWTYGLDGVLLKATRINGIQNFDVNDFGLIPIPAATW 249
           CRHHASL+ASG GK+SCFVCPYHGWTYGLDGVLLKATRINGIQNFD N+FGLIP+P ATW
Sbjct: 181 CRHHASLLASGCGKRSCFVCPYHGWTYGLDGVLLKATRINGIQNFDENNFGLIPLPVATW 240

Query: 250 GPFILLNLDKKLSSELDVDEDKVAHEWLGSRADVLSLNGVDASLSFVCRREYTIECNWKV 309
           GPF+LLNLD KLSSELDVDEDKV  EWLGS AD+LSLNGVDASLS+VCRREYTIECNWKV
Sbjct: 241 GPFVLLNLDGKLSSELDVDEDKVPGEWLGSCADLLSLNGVDASLSYVCRREYTIECNWKV 284

Query: 310 YCDNYLDGGYHVPYAHKGLASNLNIDSYSTEV 338
           +CDNYLDGGYHVPYAHKGLASNL +DSYSTE+
Sbjct: 301 FCDNYLDGGYHVPYAHKGLASNLKLDSYSTEI 284

BLAST of Spg022760 vs. NCBI nr
Match: XP_022965039.1 (choline monooxygenase, chloroplastic isoform X1 [Cucurbita moschata])

HSP 1 Score: 476.9 bits (1226), Expect = 1.5e-130
Identity = 235/332 (70.78%), Postives = 252/332 (75.90%), Query Frame = 0

Query: 10  MATLTKQIPTHFFQAPRPWITCPS----SSSRIPAAFSFRKHDSYSIEAQKLVDEFDPKI 69
           MA LTK I THFFQ         S    S +RI +  SFR  DS+ IEA KLVDEFDP+I
Sbjct: 1   MAALTKHIHTHFFQPLFASFNFQSRSYRSPTRISSTLSFRNSDSHFIEALKLVDEFDPEI 60

Query: 70  PLEKAVTPPSSWYTDPSFFALELDRVFCRGWQAVGSLPSLHCCCSSSFAALFDCICKARR 129
           PLEKAVTPPSSWYTDPSFFALELDRVF RGWQAV                          
Sbjct: 61  PLEKAVTPPSSWYTDPSFFALELDRVFHRGWQAV-------------------------- 120

Query: 130 LIIITLTILMHGSSTSCAIDLPGNVEQLKDPHDFFTGRLGNVEFVVCKDNNRKVRAFHNV 189
                                 G VEQLKDPHDFFTGRLGNVE+VVCKDNN+KVRAFHNV
Sbjct: 121 ----------------------GYVEQLKDPHDFFTGRLGNVEYVVCKDNNKKVRAFHNV 180

Query: 190 CRHHASLIASGSGKKSCFVCPYHGWTYGLDGVLLKATRINGIQNFDVNDFGLIPIPAATW 249
           CRHHASL+ASG GKKSCFVCPYHGWTYGLDG LLKATRINGIQNFDVNDFGL+P+P ATW
Sbjct: 181 CRHHASLLASGCGKKSCFVCPYHGWTYGLDGGLLKATRINGIQNFDVNDFGLVPLPVATW 240

Query: 250 GPFILLNLDKKLSSELDVDEDKVAHEWLGSRADVLSLNGVDASLSFVCRREYTIECNWKV 309
           GPF+LLNLD+KLSSEL VDED+VAHEWLGS AD+LSLNGVDASLSFVCRREYTIECNWKV
Sbjct: 241 GPFVLLNLDEKLSSELVVDEDEVAHEWLGSCADLLSLNGVDASLSFVCRREYTIECNWKV 284

Query: 310 YCDNYLDGGYHVPYAHKGLASNLNIDSYSTEV 338
           +CDNYLDGGYHVPYAHKGLASNL ++SYSTE+
Sbjct: 301 FCDNYLDGGYHVPYAHKGLASNLKLESYSTEI 284

BLAST of Spg022760 vs. NCBI nr
Match: XP_022987329.1 (choline monooxygenase, chloroplastic isoform X1 [Cucurbita maxima])

HSP 1 Score: 474.9 bits (1221), Expect = 5.6e-130
Identity = 234/332 (70.48%), Postives = 250/332 (75.30%), Query Frame = 0

Query: 10  MATLTKQIPTHFFQAPRPWITCPSSSSRIPA----AFSFRKHDSYSIEAQKLVDEFDPKI 69
           MA LTK I THFFQ         S S R P       SFR  DS+ IEA KLVDEFDP+I
Sbjct: 1   MAALTKHIHTHFFQPLFASFNFQSRSHRSPTRISPTLSFRNSDSHFIEALKLVDEFDPEI 60

Query: 70  PLEKAVTPPSSWYTDPSFFALELDRVFCRGWQAVGSLPSLHCCCSSSFAALFDCICKARR 129
           PLEKAVTPPSSWYTDPSFFALELDRVF RGWQAV                          
Sbjct: 61  PLEKAVTPPSSWYTDPSFFALELDRVFHRGWQAV-------------------------- 120

Query: 130 LIIITLTILMHGSSTSCAIDLPGNVEQLKDPHDFFTGRLGNVEFVVCKDNNRKVRAFHNV 189
                                 G VEQLKDPHDFFTGRLGNVE+VVCKDNN+KVRAFHNV
Sbjct: 121 ----------------------GYVEQLKDPHDFFTGRLGNVEYVVCKDNNKKVRAFHNV 180

Query: 190 CRHHASLIASGSGKKSCFVCPYHGWTYGLDGVLLKATRINGIQNFDVNDFGLIPIPAATW 249
           CRHHASL+ASG GKKSCFVCPYHGWTYGLDG LLKATRINGIQNFDVNDFGL+P+P ATW
Sbjct: 181 CRHHASLLASGCGKKSCFVCPYHGWTYGLDGGLLKATRINGIQNFDVNDFGLVPLPVATW 240

Query: 250 GPFILLNLDKKLSSELDVDEDKVAHEWLGSRADVLSLNGVDASLSFVCRREYTIECNWKV 309
           GPF+LLN+D+KLSSEL VDEDKVA+EWLGS AD+LSLNGVDASLSFVCRREYTIECNWKV
Sbjct: 241 GPFVLLNMDEKLSSELVVDEDKVAYEWLGSCADLLSLNGVDASLSFVCRREYTIECNWKV 284

Query: 310 YCDNYLDGGYHVPYAHKGLASNLNIDSYSTEV 338
           +CDNYLDGGYHVPYAHKGLASNL ++SYSTE+
Sbjct: 301 FCDNYLDGGYHVPYAHKGLASNLKLESYSTEI 284

BLAST of Spg022760 vs. NCBI nr
Match: KAG6589681.1 (Choline monooxygenase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023360.1 Choline monooxygenase, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 474.9 bits (1221), Expect = 5.6e-130
Identity = 234/332 (70.48%), Postives = 250/332 (75.30%), Query Frame = 0

Query: 10  MATLTKQIPTHFFQAPRPWITCPS----SSSRIPAAFSFRKHDSYSIEAQKLVDEFDPKI 69
           MA LTK I THFFQ         S    SS+RI    SFR  DS+ IEA KLVDEFDP+I
Sbjct: 1   MAALTKHIHTHFFQPLFASFNFQSRSHRSSTRISPTLSFRNSDSHFIEALKLVDEFDPEI 60

Query: 70  PLEKAVTPPSSWYTDPSFFALELDRVFCRGWQAVGSLPSLHCCCSSSFAALFDCICKARR 129
           PLEKAVTPPSSWYTDPSFF LELDRVF RGWQAV                          
Sbjct: 61  PLEKAVTPPSSWYTDPSFFDLELDRVFHRGWQAV-------------------------- 120

Query: 130 LIIITLTILMHGSSTSCAIDLPGNVEQLKDPHDFFTGRLGNVEFVVCKDNNRKVRAFHNV 189
                                 G VEQLKDPHDFFTGRLGNVE+VVCKDNN+KVRAFHNV
Sbjct: 121 ----------------------GYVEQLKDPHDFFTGRLGNVEYVVCKDNNKKVRAFHNV 180

Query: 190 CRHHASLIASGSGKKSCFVCPYHGWTYGLDGVLLKATRINGIQNFDVNDFGLIPIPAATW 249
           CRHHASL+ASG GKKSCFVCPYHGWTYGLDG LLKATRINGIQNFDVNDFGL+P+P ATW
Sbjct: 181 CRHHASLLASGCGKKSCFVCPYHGWTYGLDGGLLKATRINGIQNFDVNDFGLVPLPVATW 240

Query: 250 GPFILLNLDKKLSSELDVDEDKVAHEWLGSRADVLSLNGVDASLSFVCRREYTIECNWKV 309
           GPF+LLN+D+KLSSEL VDEDKVAHEWLGS AD+LSLNGVDASL FVCRREYTIECNWKV
Sbjct: 241 GPFVLLNMDEKLSSELVVDEDKVAHEWLGSCADLLSLNGVDASLGFVCRREYTIECNWKV 284

Query: 310 YCDNYLDGGYHVPYAHKGLASNLNIDSYSTEV 338
           +CDNYLDGGYHVPYAHKGLASNL ++SYSTE+
Sbjct: 301 FCDNYLDGGYHVPYAHKGLASNLKLESYSTEI 284

BLAST of Spg022760 vs. ExPASy Swiss-Prot
Match: Q9SZR0 (Choline monooxygenase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g29890 PE=2 SV=2)

HSP 1 Score: 330.5 bits (846), Expect = 2.2e-89
Identity = 164/337 (48.66%), Postives = 211/337 (62.61%), Query Frame = 0

Query: 10  MATLTKQIPTHF---FQAPRPWITCPSSSSRIPAAFSFRK-HDSYSI----EAQKLVDEF 69
           M TLT  +P       ++ R +    S      + FS R+ H+   +    +  KLV EF
Sbjct: 2   MTTLTATVPEFLPPSLKSTRGYFNSHSEFGVSISKFSRRRFHNPTRVFAVSDISKLVTEF 61

Query: 70  DPKIPLEKAVTPPSSWYTDPSFFALELDRVFCRGWQAVGSLPSLHCCCSSSFAALFDCIC 129
           DPKIPLE+A TPPSSWYTDP F++ ELDRVF  GWQAV                      
Sbjct: 62  DPKIPLERASTPPSSWYTDPQFYSFELDRVFYGGWQAV---------------------- 121

Query: 130 KARRLIIITLTILMHGSSTSCAIDLPGNVEQLKDPHDFFTGRLGNVEFVVCKDNNRKVRA 189
                                     G  +Q+K+  DFFTGRLG+V+FVVC+D N K+ A
Sbjct: 122 --------------------------GYSDQIKESRDFFTGRLGDVDFVVCRDENGKIHA 181

Query: 190 FHNVCRHHASLIASGSGKKSCFVCPYHGWTYGLDGVLLKATRINGIQNFDVNDFGLIPIP 249
           FHNVC HHAS++ASG+G+KSCFVC YHGWTY L G L+KATR++GIQNF +++ GL P+ 
Sbjct: 182 FHNVCSHHASILASGNGRKSCFVCLYHGWTYSLSGSLVKATRMSGIQNFSLSEMGLKPLR 241

Query: 250 AATWGPFILLNLDKKLSSELDVDEDK-VAHEWLGSRADVLSLNGVDASLSFVCRREYTIE 309
            A WGPF+LL +    S + +V+ D+ VA EWLG+    LS  GVD+ LS++CRREYTI+
Sbjct: 242 VAVWGPFVLLKVTAATSRKGEVETDELVASEWLGTSVGRLSQGGVDSPLSYICRREYTID 290

Query: 310 CNWKVYCDNYLDGGYHVPYAHKGLASNLNIDSYSTEV 338
           CNWKV+CDNYLDGGYHVPYAHKGL S L++++YST +
Sbjct: 302 CNWKVFCDNYLDGGYHVPYAHKGLMSGLDLETYSTTI 290

BLAST of Spg022760 vs. ExPASy Swiss-Prot
Match: O22553 (Choline monooxygenase, chloroplastic OS=Beta vulgaris OX=161934 GN=CMO PE=2 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 3.3e-72
Identity = 126/283 (44.52%), Postives = 170/283 (60.07%), Query Frame = 0

Query: 55  QKLVDEFDPKIPLEKAVTPPSSWYTDPSFFALELDRVFCRGWQAVGSLPSLHCCCSSSFA 114
           + LV EFDP+IP E A+TPPS+WYT+P+F++ EL+R+F +GWQ                 
Sbjct: 86  RSLVHEFDPEIPPEDALTPPSTWYTEPAFYSHELERIFYKGWQ----------------- 145

Query: 115 ALFDCICKARRLIIITLTILMHGSSTSCAIDLPGNVEQLKDPHDFFTGRLGNVEFVVCKD 174
                                          + G  EQ+K+ + +FTG LGNVE++V +D
Sbjct: 146 -------------------------------VAGYSEQVKEKNQYFTGSLGNVEYLVSRD 205

Query: 175 NNRKVRAFHNVCRHHASLIASGSGKKSCFVCPYHGWTYGLDGVLLKATRINGIQNFDVND 234
              ++ AFHNVC H AS++A GSGKKSCFVCPYHGW YGLDG L KA++    QN D  +
Sbjct: 206 GQGELHAFHNVCTHRASILACGSGKKSCFVCPYHGWVYGLDGSLAKASKATETQNLDPKE 265

Query: 235 FGLIPIPAATWGPFILLNLDKKLSSELDVDEDKVAHEWLGSRADVLSLNGVDASLSFVCR 294
            GL P+  A WGPFIL++LD+ L +  D     V  EW+G  A+ +  +  D +L F  R
Sbjct: 266 LGLAPLKVAEWGPFILISLDRSLDANAD-----VGTEWIGKSAEDVKAHAFDPNLKFTHR 315

Query: 295 REYTIECNWKVYCDNYLDGGYHVPYAHKGLASNLNIDSYSTEV 338
            E+ +ECNWKV+CDNYLD  YHVPYAHK  A+ L+ D+Y+TE+
Sbjct: 326 SEFPMECNWKVFCDNYLDSSYHVPYAHKYYAAELDFDTYNTEM 315

BLAST of Spg022760 vs. ExPASy Swiss-Prot
Match: O04121 (Choline monooxygenase, chloroplastic OS=Spinacia oleracea OX=3562 GN=CMO PE=1 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 3.3e-72
Identity = 127/283 (44.88%), Postives = 168/283 (59.36%), Query Frame = 0

Query: 55  QKLVDEFDPKIPLEKAVTPPSSWYTDPSFFALELDRVFCRGWQAVGSLPSLHCCCSSSFA 114
           Q LV EFDP+IP E A TPPSSWYT+P+F++ EL+R+F +GWQ  G              
Sbjct: 79  QSLVHEFDPQIPPEDAHTPPSSWYTEPAFYSHELERIFYKGWQVAGI------------- 138

Query: 115 ALFDCICKARRLIIITLTILMHGSSTSCAIDLPGNVEQLKDPHDFFTGRLGNVEFVVCKD 174
                                               +Q+K+P+ +FTG LGNVE++V +D
Sbjct: 139 -----------------------------------SDQIKEPNQYFTGSLGNVEYLVSRD 198

Query: 175 NNRKVRAFHNVCRHHASLIASGSGKKSCFVCPYHGWTYGLDGVLLKATRINGIQNFDVND 234
              KV AFHNVC H AS++A GSGKKSCFVCPYHGW YG+DG L KA++    QN D  +
Sbjct: 199 GEGKVHAFHNVCTHRASILACGSGKKSCFVCPYHGWVYGMDGSLAKASKAKPEQNLDPKE 258

Query: 235 FGLIPIPAATWGPFILLNLDKKLSSELDVDEDKVAHEWLGSRADVLSLNGVDASLSFVCR 294
            GL+P+  A WGPF+L++LD+ L    D     V  EWLG+ A+ +  +  D SL F+ R
Sbjct: 259 LGLVPLKVAVWGPFVLISLDRSLEEGGD-----VGTEWLGTSAEDVKAHAFDPSLQFIHR 308

Query: 295 REYTIECNWKVYCDNYLDGGYHVPYAHKGLASNLNIDSYSTEV 338
            E+ +E NWK++ DNYLD  YHVPYAHK  A+ LN D+Y T++
Sbjct: 319 SEFPMESNWKIFSDNYLDSSYHVPYAHKYYATELNFDTYDTQM 308

BLAST of Spg022760 vs. ExPASy Swiss-Prot
Match: Q93XE1 (Choline monooxygenase, chloroplastic OS=Amaranthus tricolor OX=29722 GN=CMO PE=2 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 4.3e-72
Identity = 127/304 (41.78%), Postives = 179/304 (58.88%), Query Frame = 0

Query: 34  SSSRIPAAFSFRKHDSYSIEAQKLVDEFDPKIPLEKAVTPPSSWYTDPSFFALELDRVFC 93
           +S  IP++ +     + +   ++++ EFDPK+P E   TPPS+WYTDPS ++ ELDR+F 
Sbjct: 61  ASPVIPSSINSNNITTTTPNIKRIIHEFDPKVPAEDGFTPPSTWYTDPSLYSHELDRIFS 120

Query: 94  RGWQAVGSLPSLHCCCSSSFAALFDCICKARRLIIITLTILMHGSSTSCAIDLPGNVEQL 153
           +GWQ                                                + G  +Q+
Sbjct: 121 KGWQ------------------------------------------------VAGYSDQI 180

Query: 154 KDPHDFFTGRLGNVEFVVCKDNNRKVRAFHNVCRHHASLIASGSGKKSCFVCPYHGWTYG 213
           K+P+ +FTG LGNVE++VC+D   KV AFHNVC H AS++A G+GKKSCFVCPYHGW +G
Sbjct: 181 KEPNQYFTGSLGNVEYLVCRDGQGKVHAFHNVCTHRASILACGTGKKSCFVCPYHGWVFG 240

Query: 214 LDGVLLKATRINGIQNFDVNDFGLIPIPAATWGPFILLNLDKKLSSELDVDEDKVAHEWL 273
           LDG L+KAT+    Q FD  + GL+ +  A WGPF+L++LD+  S       + V  EW+
Sbjct: 241 LDGSLMKATKTEN-QVFDPKELGLVTLKVAIWGPFVLISLDRSGSE----GTEDVGKEWI 300

Query: 274 GSRADVLSLNGVDASLSFVCRREYTIECNWKVYCDNYLDGGYHVPYAHKGLASNLNIDSY 333
           GS A+ +  +  D SL F+ R E+ +E NWKV+CDNYLD  YHVPYAHK  A+ L+ D+Y
Sbjct: 301 GSCAEEVKKHAFDPSLQFINRSEFPMESNWKVFCDNYLDSAYHVPYAHKYYAAELDFDTY 311

Query: 334 STEV 338
            T++
Sbjct: 361 KTDL 311

BLAST of Spg022760 vs. ExPASy Swiss-Prot
Match: Q9LKN0 (Choline monooxygenase, chloroplastic OS=Atriplex hortensis OX=34272 GN=CMO PE=2 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 2.3e-70
Identity = 125/283 (44.17%), Postives = 172/283 (60.78%), Query Frame = 0

Query: 55  QKLVDEFDPKIPLEKAVTPPSSWYTDPSFFALELDRVFCRGWQAVGSLPSLHCCCSSSFA 114
           Q LV +FDP +P E A+TPPSSWYT+P+F+A ELDR+F +GWQ                 
Sbjct: 80  QSLVKDFDPLVPAEDALTPPSSWYTEPAFYAHELDRIFYKGWQ----------------- 139

Query: 115 ALFDCICKARRLIIITLTILMHGSSTSCAIDLPGNVEQLKDPHDFFTGRLGNVEFVVCKD 174
                                          + G  +Q+K+ + +FTG LGNVE++VC+D
Sbjct: 140 -------------------------------VAGYSDQVKEANQYFTGTLGNVEYLVCRD 199

Query: 175 NNRKVRAFHNVCRHHASLIASGSGKKSCFVCPYHGWTYGLDGVLLKATRINGIQNFDVND 234
              KV AFHNVC H AS++A GSGKKSCFVCPYHGW YG++G L KA++    Q+ + ++
Sbjct: 200 GEGKVHAFHNVCTHRASILACGSGKKSCFVCPYHGWVYGMNGSLTKASKATPEQSLNPDE 259

Query: 235 FGLIPIPAATWGPFILLNLDKKLSSELDVDEDKVAHEWLGSRADVLSLNGVDASLSFVCR 294
            GL+P+  A WGPFIL++LD+   S  +V +  V  EWLGS A+ +  +  D +L F+ R
Sbjct: 260 LGLVPLKVAVWGPFILISLDR---SSREVGD--VGSEWLGSCAEDVKAHAFDPNLQFINR 309

Query: 295 REYTIECNWKVYCDNYLDGGYHVPYAHKGLASNLNIDSYSTEV 338
            E+ IE NWK++ DNYLD  YHVPYAHK  A+ L+ D+Y T++
Sbjct: 320 SEFPIESNWKIFSDNYLDSSYHVPYAHKYYATELDFDTYQTDM 309

BLAST of Spg022760 vs. ExPASy TrEMBL
Match: A0A1S4DUW5 (choline monooxygenase, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103487183 PE=4 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 1.1e-136
Identity = 243/332 (73.19%), Postives = 265/332 (79.82%), Query Frame = 0

Query: 10  MATLTKQIPTHFFQAPR---PWITC-PSSSSRIPAAFSFRKHDSYSIEAQKLVDEFDPKI 69
           MATLTK I  HFFQ P     +  C   S SRI A+ SFR  DS  IEA+KLVD+FDP+I
Sbjct: 1   MATLTKHIQIHFFQPPSISFNFHFCNHRSPSRISASLSFRSPDSRFIEARKLVDDFDPEI 60

Query: 70  PLEKAVTPPSSWYTDPSFFALELDRVFCRGWQAVGSLPSLHCCCSSSFAALFDCICKARR 129
           PLEKA+TPPSSWY DPSFFALELDRVF RGWQA                           
Sbjct: 61  PLEKALTPPSSWYIDPSFFALELDRVFYRGWQA--------------------------- 120

Query: 130 LIIITLTILMHGSSTSCAIDLPGNVEQLKDPHDFFTGRLGNVEFVVCKDNNRKVRAFHNV 189
             IITLTIL HGSST CAI L G VEQLKD HDFFTGRLGNVE+VVCKDNNRKVRAFHNV
Sbjct: 121 --IITLTILRHGSSTFCAIGLSGYVEQLKDAHDFFTGRLGNVEYVVCKDNNRKVRAFHNV 180

Query: 190 CRHHASLIASGSGKKSCFVCPYHGWTYGLDGVLLKATRINGIQNFDVNDFGLIPIPAATW 249
           CRHHASL+A+G GKKSCFVCPYHGWTYGLDG+LLKATRINGIQNF+ NDFGL+P+P A W
Sbjct: 181 CRHHASLLATGCGKKSCFVCPYHGWTYGLDGILLKATRINGIQNFNENDFGLVPLPVAMW 240

Query: 250 GPFILLNLDKKLSSELDVDEDKVAHEWLGSRADVLSLNGVDASLSFVCRREYTIECNWKV 309
           GPF+LLNLD KLSS+ DVDEDKVA EWLG+ ADVL LNGVDASLS+VCRREYTI+CNWKV
Sbjct: 241 GPFVLLNLDGKLSSKPDVDEDKVAREWLGTCADVLRLNGVDASLSYVCRREYTIDCNWKV 300

Query: 310 YCDNYLDGGYHVPYAHKGLASNLNIDSYSTEV 338
           +CDNYLDGGYHVPYAHKGLASNLN++SYSTE+
Sbjct: 301 FCDNYLDGGYHVPYAHKGLASNLNLESYSTEL 303

BLAST of Spg022760 vs. ExPASy TrEMBL
Match: A0A6J1HPX3 (Choline monooxygenase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111464984 PE=3 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 7.2e-131
Identity = 235/332 (70.78%), Postives = 252/332 (75.90%), Query Frame = 0

Query: 10  MATLTKQIPTHFFQAPRPWITCPS----SSSRIPAAFSFRKHDSYSIEAQKLVDEFDPKI 69
           MA LTK I THFFQ         S    S +RI +  SFR  DS+ IEA KLVDEFDP+I
Sbjct: 1   MAALTKHIHTHFFQPLFASFNFQSRSYRSPTRISSTLSFRNSDSHFIEALKLVDEFDPEI 60

Query: 70  PLEKAVTPPSSWYTDPSFFALELDRVFCRGWQAVGSLPSLHCCCSSSFAALFDCICKARR 129
           PLEKAVTPPSSWYTDPSFFALELDRVF RGWQAV                          
Sbjct: 61  PLEKAVTPPSSWYTDPSFFALELDRVFHRGWQAV-------------------------- 120

Query: 130 LIIITLTILMHGSSTSCAIDLPGNVEQLKDPHDFFTGRLGNVEFVVCKDNNRKVRAFHNV 189
                                 G VEQLKDPHDFFTGRLGNVE+VVCKDNN+KVRAFHNV
Sbjct: 121 ----------------------GYVEQLKDPHDFFTGRLGNVEYVVCKDNNKKVRAFHNV 180

Query: 190 CRHHASLIASGSGKKSCFVCPYHGWTYGLDGVLLKATRINGIQNFDVNDFGLIPIPAATW 249
           CRHHASL+ASG GKKSCFVCPYHGWTYGLDG LLKATRINGIQNFDVNDFGL+P+P ATW
Sbjct: 181 CRHHASLLASGCGKKSCFVCPYHGWTYGLDGGLLKATRINGIQNFDVNDFGLVPLPVATW 240

Query: 250 GPFILLNLDKKLSSELDVDEDKVAHEWLGSRADVLSLNGVDASLSFVCRREYTIECNWKV 309
           GPF+LLNLD+KLSSEL VDED+VAHEWLGS AD+LSLNGVDASLSFVCRREYTIECNWKV
Sbjct: 241 GPFVLLNLDEKLSSELVVDEDEVAHEWLGSCADLLSLNGVDASLSFVCRREYTIECNWKV 284

Query: 310 YCDNYLDGGYHVPYAHKGLASNLNIDSYSTEV 338
           +CDNYLDGGYHVPYAHKGLASNL ++SYSTE+
Sbjct: 301 FCDNYLDGGYHVPYAHKGLASNLKLESYSTEI 284

BLAST of Spg022760 vs. ExPASy TrEMBL
Match: A0A6J1JGJ2 (Choline monooxygenase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111484916 PE=3 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 2.7e-130
Identity = 234/332 (70.48%), Postives = 250/332 (75.30%), Query Frame = 0

Query: 10  MATLTKQIPTHFFQAPRPWITCPSSSSRIPA----AFSFRKHDSYSIEAQKLVDEFDPKI 69
           MA LTK I THFFQ         S S R P       SFR  DS+ IEA KLVDEFDP+I
Sbjct: 1   MAALTKHIHTHFFQPLFASFNFQSRSHRSPTRISPTLSFRNSDSHFIEALKLVDEFDPEI 60

Query: 70  PLEKAVTPPSSWYTDPSFFALELDRVFCRGWQAVGSLPSLHCCCSSSFAALFDCICKARR 129
           PLEKAVTPPSSWYTDPSFFALELDRVF RGWQAV                          
Sbjct: 61  PLEKAVTPPSSWYTDPSFFALELDRVFHRGWQAV-------------------------- 120

Query: 130 LIIITLTILMHGSSTSCAIDLPGNVEQLKDPHDFFTGRLGNVEFVVCKDNNRKVRAFHNV 189
                                 G VEQLKDPHDFFTGRLGNVE+VVCKDNN+KVRAFHNV
Sbjct: 121 ----------------------GYVEQLKDPHDFFTGRLGNVEYVVCKDNNKKVRAFHNV 180

Query: 190 CRHHASLIASGSGKKSCFVCPYHGWTYGLDGVLLKATRINGIQNFDVNDFGLIPIPAATW 249
           CRHHASL+ASG GKKSCFVCPYHGWTYGLDG LLKATRINGIQNFDVNDFGL+P+P ATW
Sbjct: 181 CRHHASLLASGCGKKSCFVCPYHGWTYGLDGGLLKATRINGIQNFDVNDFGLVPLPVATW 240

Query: 250 GPFILLNLDKKLSSELDVDEDKVAHEWLGSRADVLSLNGVDASLSFVCRREYTIECNWKV 309
           GPF+LLN+D+KLSSEL VDEDKVA+EWLGS AD+LSLNGVDASLSFVCRREYTIECNWKV
Sbjct: 241 GPFVLLNMDEKLSSELVVDEDKVAYEWLGSCADLLSLNGVDASLSFVCRREYTIECNWKV 284

Query: 310 YCDNYLDGGYHVPYAHKGLASNLNIDSYSTEV 338
           +CDNYLDGGYHVPYAHKGLASNL ++SYSTE+
Sbjct: 301 FCDNYLDGGYHVPYAHKGLASNLKLESYSTEI 284

BLAST of Spg022760 vs. ExPASy TrEMBL
Match: A0A6J1JIK0 (Choline monooxygenase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111484916 PE=3 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 2.7e-130
Identity = 234/332 (70.48%), Postives = 250/332 (75.30%), Query Frame = 0

Query: 10  MATLTKQIPTHFFQAPRPWITCPSSSSRIPA----AFSFRKHDSYSIEAQKLVDEFDPKI 69
           MA LTK I THFFQ         S S R P       SFR  DS+ IEA KLVDEFDP+I
Sbjct: 1   MAALTKHIHTHFFQPLFASFNFQSRSHRSPTRISPTLSFRNSDSHFIEALKLVDEFDPEI 60

Query: 70  PLEKAVTPPSSWYTDPSFFALELDRVFCRGWQAVGSLPSLHCCCSSSFAALFDCICKARR 129
           PLEKAVTPPSSWYTDPSFFALELDRVF RGWQAV                          
Sbjct: 61  PLEKAVTPPSSWYTDPSFFALELDRVFHRGWQAV-------------------------- 120

Query: 130 LIIITLTILMHGSSTSCAIDLPGNVEQLKDPHDFFTGRLGNVEFVVCKDNNRKVRAFHNV 189
                                 G VEQLKDPHDFFTGRLGNVE+VVCKDNN+KVRAFHNV
Sbjct: 121 ----------------------GYVEQLKDPHDFFTGRLGNVEYVVCKDNNKKVRAFHNV 180

Query: 190 CRHHASLIASGSGKKSCFVCPYHGWTYGLDGVLLKATRINGIQNFDVNDFGLIPIPAATW 249
           CRHHASL+ASG GKKSCFVCPYHGWTYGLDG LLKATRINGIQNFDVNDFGL+P+P ATW
Sbjct: 181 CRHHASLLASGCGKKSCFVCPYHGWTYGLDGGLLKATRINGIQNFDVNDFGLVPLPVATW 240

Query: 250 GPFILLNLDKKLSSELDVDEDKVAHEWLGSRADVLSLNGVDASLSFVCRREYTIECNWKV 309
           GPF+LLN+D+KLSSEL VDEDKVA+EWLGS AD+LSLNGVDASLSFVCRREYTIECNWKV
Sbjct: 241 GPFVLLNMDEKLSSELVVDEDKVAYEWLGSCADLLSLNGVDASLSFVCRREYTIECNWKV 284

Query: 310 YCDNYLDGGYHVPYAHKGLASNLNIDSYSTEV 338
           +CDNYLDGGYHVPYAHKGLASNL ++SYSTE+
Sbjct: 301 FCDNYLDGGYHVPYAHKGLASNLKLESYSTEI 284

BLAST of Spg022760 vs. ExPASy TrEMBL
Match: A0A6J1C1T8 (Choline monooxygenase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111007490 PE=3 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 9.7e-128
Identity = 231/332 (69.58%), Postives = 248/332 (74.70%), Query Frame = 0

Query: 10  MATLTKQIPTHFFQAPRPWITCPSSSSR----IPAAFSFRKHDSYSIEAQKLVDEFDPKI 69
           MAT+ KQIPTHFFQ   P I  PS + R    I A  +FR  DS  +EAQKLV EFDP+I
Sbjct: 1   MATIIKQIPTHFFQP--PGIATPSINHRPSMPISAVSTFRNPDSRFVEAQKLVHEFDPEI 60

Query: 70  PLEKAVTPPSSWYTDPSFFALELDRVFCRGWQAVGSLPSLHCCCSSSFAALFDCICKARR 129
           PLEKA+TPPSSWYTDPSFF LELDRVF RGWQAV                          
Sbjct: 61  PLEKALTPPSSWYTDPSFFTLELDRVFYRGWQAV-------------------------- 120

Query: 130 LIIITLTILMHGSSTSCAIDLPGNVEQLKDPHDFFTGRLGNVEFVVCKDNNRKVRAFHNV 189
                                 G VEQLKDPHDFFTGRLGNVEFVVCKDNN KVRAFHNV
Sbjct: 121 ----------------------GYVEQLKDPHDFFTGRLGNVEFVVCKDNNGKVRAFHNV 180

Query: 190 CRHHASLIASGSGKKSCFVCPYHGWTYGLDGVLLKATRINGIQNFDVNDFGLIPIPAATW 249
           CRHHASL+ASGSGKKSCFVCPYHGWTYGLDGVLLKATRI GIQNFDVNDFGLIP+  ATW
Sbjct: 181 CRHHASLLASGSGKKSCFVCPYHGWTYGLDGVLLKATRIKGIQNFDVNDFGLIPLLVATW 240

Query: 250 GPFILLNLDKKLSSELDVDEDKVAHEWLGSRADVLSLNGVDASLSFVCRREYTIECNWKV 309
           GPF+LLNLDK+L SE +VDEDKVA EWLGS  DVLSLNGV+ SLS+VCRREYTIECNWKV
Sbjct: 241 GPFVLLNLDKELPSEQEVDEDKVAREWLGSCVDVLSLNGVNTSLSYVCRREYTIECNWKV 282

Query: 310 YCDNYLDGGYHVPYAHKGLASNLNIDSYSTEV 338
           +CDNYLDGGYHVPYAHKGLASNL ++SY TE+
Sbjct: 301 FCDNYLDGGYHVPYAHKGLASNLKLESYLTEI 282

BLAST of Spg022760 vs. TAIR 10
Match: AT4G29890.1 (choline monooxygenase, putative (CMO-like) )

HSP 1 Score: 330.5 bits (846), Expect = 1.6e-90
Identity = 164/337 (48.66%), Postives = 211/337 (62.61%), Query Frame = 0

Query: 10  MATLTKQIPTHF---FQAPRPWITCPSSSSRIPAAFSFRK-HDSYSI----EAQKLVDEF 69
           M TLT  +P       ++ R +    S      + FS R+ H+   +    +  KLV EF
Sbjct: 2   MTTLTATVPEFLPPSLKSTRGYFNSHSEFGVSISKFSRRRFHNPTRVFAVSDISKLVTEF 61

Query: 70  DPKIPLEKAVTPPSSWYTDPSFFALELDRVFCRGWQAVGSLPSLHCCCSSSFAALFDCIC 129
           DPKIPLE+A TPPSSWYTDP F++ ELDRVF  GWQAV                      
Sbjct: 62  DPKIPLERASTPPSSWYTDPQFYSFELDRVFYGGWQAV---------------------- 121

Query: 130 KARRLIIITLTILMHGSSTSCAIDLPGNVEQLKDPHDFFTGRLGNVEFVVCKDNNRKVRA 189
                                     G  +Q+K+  DFFTGRLG+V+FVVC+D N K+ A
Sbjct: 122 --------------------------GYSDQIKESRDFFTGRLGDVDFVVCRDENGKIHA 181

Query: 190 FHNVCRHHASLIASGSGKKSCFVCPYHGWTYGLDGVLLKATRINGIQNFDVNDFGLIPIP 249
           FHNVC HHAS++ASG+G+KSCFVC YHGWTY L G L+KATR++GIQNF +++ GL P+ 
Sbjct: 182 FHNVCSHHASILASGNGRKSCFVCLYHGWTYSLSGSLVKATRMSGIQNFSLSEMGLKPLR 241

Query: 250 AATWGPFILLNLDKKLSSELDVDEDK-VAHEWLGSRADVLSLNGVDASLSFVCRREYTIE 309
            A WGPF+LL +    S + +V+ D+ VA EWLG+    LS  GVD+ LS++CRREYTI+
Sbjct: 242 VAVWGPFVLLKVTAATSRKGEVETDELVASEWLGTSVGRLSQGGVDSPLSYICRREYTID 290

Query: 310 CNWKVYCDNYLDGGYHVPYAHKGLASNLNIDSYSTEV 338
           CNWKV+CDNYLDGGYHVPYAHKGL S L++++YST +
Sbjct: 302 CNWKVFCDNYLDGGYHVPYAHKGLMSGLDLETYSTTI 290

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_016899776.12.4e-13673.19PREDICTED: choline monooxygenase, chloroplastic isoform X1 [Cucumis melo][more]
XP_038880722.11.6e-13271.99choline monooxygenase, chloroplastic [Benincasa hispida][more]
XP_022965039.11.5e-13070.78choline monooxygenase, chloroplastic isoform X1 [Cucurbita moschata][more]
XP_022987329.15.6e-13070.48choline monooxygenase, chloroplastic isoform X1 [Cucurbita maxima][more]
KAG6589681.15.6e-13070.48Choline monooxygenase, chloroplastic, partial [Cucurbita argyrosperma subsp. sor... [more]
Match NameE-valueIdentityDescription
Q9SZR02.2e-8948.66Choline monooxygenase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g2989... [more]
O225533.3e-7244.52Choline monooxygenase, chloroplastic OS=Beta vulgaris OX=161934 GN=CMO PE=2 SV=1[more]
O041213.3e-7244.88Choline monooxygenase, chloroplastic OS=Spinacia oleracea OX=3562 GN=CMO PE=1 SV... [more]
Q93XE14.3e-7241.78Choline monooxygenase, chloroplastic OS=Amaranthus tricolor OX=29722 GN=CMO PE=2... [more]
Q9LKN02.3e-7044.17Choline monooxygenase, chloroplastic OS=Atriplex hortensis OX=34272 GN=CMO PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A1S4DUW51.1e-13673.19choline monooxygenase, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A6J1HPX37.2e-13170.78Choline monooxygenase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC1114649... [more]
A0A6J1JGJ22.7e-13070.48Choline monooxygenase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111484916... [more]
A0A6J1JIK02.7e-13070.48Choline monooxygenase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111484916... [more]
A0A6J1C1T89.7e-12869.58Choline monooxygenase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111007... [more]
Match NameE-valueIdentityDescription
AT4G29890.11.6e-9048.66choline monooxygenase, putative (CMO-like) [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015879Aromatic-ring-hydroxylating dioxygenase, alpha subunit, C-terminal domainPFAMPF00848Ring_hydroxyl_Acoord: 294..329
e-value: 3.0E-8
score: 33.9
IPR017941Rieske [2Fe-2S] iron-sulphur domainPFAMPF00355Rieskecoord: 150..222
e-value: 2.7E-14
score: 52.9
IPR017941Rieske [2Fe-2S] iron-sulphur domainPROSITEPS51296RIESKEcoord: 145..251
score: 22.374025
IPR036922Rieske [2Fe-2S] iron-sulphur domain superfamilyGENE3D2.102.10.10coord: 146..259
e-value: 2.0E-28
score: 100.3
IPR036922Rieske [2Fe-2S] iron-sulphur domain superfamilySUPERFAMILY50022ISP domaincoord: 59..104
IPR036922Rieske [2Fe-2S] iron-sulphur domain superfamilySUPERFAMILY50022ISP domaincoord: 149..254
NoneNo IPR availableGENE3D3.90.380.10coord: 260..338
e-value: 2.8E-12
score: 48.6
NoneNo IPR availablePANTHERPTHR43756:SF5CHOLINE MONOOXYGENASE, CHLOROPLASTICcoord: 148..337
coord: 52..101
NoneNo IPR availableSUPERFAMILY55961Bet v1-likecoord: 271..329
IPR044637Aromatic-ring-hydroxylating dioxygenasePANTHERPTHR43756CHOLINE MONOOXYGENASE, CHLOROPLASTICcoord: 148..337
coord: 52..101

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg022760.1Spg022760.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019285 glycine betaine biosynthetic process from choline
biological_process GO:0044237 cellular metabolic process
cellular_component GO:0009570 chloroplast stroma
cellular_component GO:0016020 membrane
cellular_component GO:0031967 organelle envelope
molecular_function GO:0051537 2 iron, 2 sulfur cluster binding
molecular_function GO:0019133 choline monooxygenase activity
molecular_function GO:0005506 iron ion binding