Spg022739 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg022739
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionDUF4220 domain-containing protein
Locationscaffold2: 15502061 .. 15504118 (-)
RNA-Seq ExpressionSpg022739
SyntenySpg022739
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATACATCTGCTTTCTGGGATTTTATGTACGAATGGGAGCTTGTGTTTTTGCTCGTCGTAAGTTTATCTCTACAGTTGGTTCTGGTTCCAATGGGGTCGAAGAGAAAATACAGTTCCAACAACTGCCTCCGCCTGTTTCTCTTGATTGCATATTCTTCTGCTGATTGGATCGTCATGGTTTCTTTGGGCATCGTCATTGAAAAATACGGCGGCGGCTGCTACGATGAATTTTCAGATCCCATATACATCATTAGAGCCTTTTGGATGCCATTTTTACTATTCCACCTCGGTGGCTCCGACACCATCACTGCCTACTCCATTCAAGACAACGACCTCTGGCTAAGAGCTCTTTTTCGCTTCATCACTAACCTTCTCCTTTCGTTTTACATCTTTATGCTAGCCCTGAACCGACCTAAGGCCCTCAACTATATAGCCATTCCCGTGTTTGTTGCTGGGATTATCAAGTATGCTCAGAAGATTTGTGCTTTGAGATCTGCTTCTGGCCAACGCTTGCGAGATTTGTTGGTTTCACCTCCTCCTCCTTTCAGACCCAACACTCAAAATTTTGCATTTCCCATCAAAATCACTTCCACAGAGATGGACCAGTTGAATGTTTCTAATAATAACGACCCATATTTCAGCGATCTTCACATGCTACATCTTGCTTACTGTTTCTTCAAGAGAGACAAGCGGTTGTTTGTAGATTTGGATTTGAGCTCCTATCATCTTCAACAAAGCCTTTACTACTTCGAGAAATTTGATTCTAAAACTGCGTTCAAAGTCATTGAGCTCGAGCTCGGGTTTATATACGATTTCTTCTACACAAAAGCCTTTGTCATTCATTCTCGATGGGGTCGTGTTCTTCGCCTCACCAGCGTATCTTCCCTTGTCATCGCCATGGTCGCCTTCAGCTGCATGAGTGACTGGCAGGATTATCCTTTAAAGTATGTGAGATTGACATTTATCCTCTTCTCTGCAGCTTTGGGGCTTGAGATCTATTCACTTCTCCTATTTCTACACTCAGATTGGAACGTAATTTCTTTGACTCGAAGCTCTTACTCCTTGCCTCGTTTGATCTTTAAACTCATCTCTCATTGTGGATGGTCTGTTTGGAAGAGAAGATGGTCGAATTCCACTTCGCAGTACAATATAATATCTCGTTGCTTGAAAGAGAAAAACGACGGACGTTATTATTATCGCAAGTTCTTAAGATCAATTTCTTGCATCACCAAAATCACGGCTGCTAATTCACGATGCATCTCTAATGACCTTCAAACGCGGATCTTCGAACAGCTAAAGGAGAAGAAGTTGGAAGCCAATGAAGCCAACAAGAAGCTACCTGGCTGGATTTTAAGAAAGCACAACTGTTATAATCAGCTTGGTTGGAGCCTAAAATTAGATTTCGACGAAAGCATCCTCCTTTGGCACATCGCCACAGATATTTGCTATCATAATAATTCTGATGCAGCATCAATTGATTCTTCTCTTCTTGAAGACAGCGTATTGTTGTCTGATTTTTTGACCGACATTTTAGCACAACGTCCATGGTTGTTTCCAAATGGGATGAGTCAAATAAGGCTTAGCGCCACAAATGACGATGTCGTTGAATTTCTACAGAAGAAAAAGTTGGTAGAAATAGCTAGTGATGCTTGTAAGAGAATGTTGGATTTGGAATTGCAGATTCCTCACGAAGAAAAACGGCGACGCCACTCAATGTTCTTTGATGGTTGTCGGCTAGCGCAGCAGCTTCAAGAGCTACGAGGGTCTGAGAGGTGGGAGATAATCAGCCATGTGTGGGTGGAGTTGCTTGCCAATATTTCATGTGAATGCGAATGGTACGAACATGCTAAGCAGCTTAGGCAGGGCGGGAGCCTGCTCACTCATGTTTGGCTTTTGATGCAACATCTTGGCTACATCAAGCAGGACAGCTTCTTCACCATGCAAGATCAAGAAGAAGAAGATACAGAACTAGTCGATGAAATCCTAAATAGTGATGTGGTGGGTCATATGTTGGATATTGTAGCTCGTGTGACAGGTCTCAATTCAAATTCCTAG

mRNA sequence

ATGGATACATCTGCTTTCTGGGATTTTATGTACGAATGGGAGCTTGTGTTTTTGCTCGTCGTAAGTTTATCTCTACAGTTGGTTCTGGTTCCAATGGGGTCGAAGAGAAAATACAGTTCCAACAACTGCCTCCGCCTGTTTCTCTTGATTGCATATTCTTCTGCTGATTGGATCGTCATGGTTTCTTTGGGCATCGTCATTGAAAAATACGGCGGCGGCTGCTACGATGAATTTTCAGATCCCATATACATCATTAGAGCCTTTTGGATGCCATTTTTACTATTCCACCTCGGTGGCTCCGACACCATCACTGCCTACTCCATTCAAGACAACGACCTCTGGCTAAGAGCTCTTTTTCGCTTCATCACTAACCTTCTCCTTTCGTTTTACATCTTTATGCTAGCCCTGAACCGACCTAAGGCCCTCAACTATATAGCCATTCCCGTGTTTGTTGCTGGGATTATCAAGTATGCTCAGAAGATTTGTGCTTTGAGATCTGCTTCTGGCCAACGCTTGCGAGATTTGTTGGTTTCACCTCCTCCTCCTTTCAGACCCAACACTCAAAATTTTGCATTTCCCATCAAAATCACTTCCACAGAGATGGACCAGTTGAATGTTTCTAATAATAACGACCCATATTTCAGCGATCTTCACATGCTACATCTTGCTTACTGTTTCTTCAAGAGAGACAAGCGGTTGTTTGTAGATTTGGATTTGAGCTCCTATCATCTTCAACAAAGCCTTTACTACTTCGAGAAATTTGATTCTAAAACTGCGTTCAAAGTCATTGAGCTCGAGCTCGGGTTTATATACGATTTCTTCTACACAAAAGCCTTTGTCATTCATTCTCGATGGGGTCGTGTTCTTCGCCTCACCAGCGTATCTTCCCTTGTCATCGCCATGGTCGCCTTCAGCTGCATGAGTGACTGGCAGGATTATCCTTTAAAGTATGTGAGATTGACATTTATCCTCTTCTCTGCAGCTTTGGGGCTTGAGATCTATTCACTTCTCCTATTTCTACACTCAGATTGGAACGTAATTTCTTTGACTCGAAGCTCTTACTCCTTGCCTCGTTTGATCTTTAAACTCATCTCTCATTGTGGATGGTCTGTTTGGAAGAGAAGATGGTCGAATTCCACTTCGCAGTACAATATAATATCTCGTTGCTTGAAAGAGAAAAACGACGGACGTTATTATTATCGCAAGTTCTTAAGATCAATTTCTTGCATCACCAAAATCACGGCTGCTAATTCACGATGCATCTCTAATGACCTTCAAACGCGGATCTTCGAACAGCTAAAGGAGAAGAAGTTGGAAGCCAATGAAGCCAACAAGAAGCTACCTGGCTGGATTTTAAGAAAGCACAACTGTTATAATCAGCTTGGTTGGAGCCTAAAATTAGATTTCGACGAAAGCATCCTCCTTTGGCACATCGCCACAGATATTTGCTATCATAATAATTCTGATGCAGCATCAATTGATTCTTCTCTTCTTGAAGACAGCGTATTGTTGTCTGATTTTTTGACCGACATTTTAGCACAACGTCCATGGTTGTTTCCAAATGGGATGAGTCAAATAAGGCTTAGCGCCACAAATGACGATGTCGTTGAATTTCTACAGAAGAAAAAGTTGGTAGAAATAGCTAGTGATGCTTGTAAGAGAATGTTGGATTTGGAATTGCAGATTCCTCACGAAGAAAAACGGCGACGCCACTCAATGTTCTTTGATGGTTGTCGGCTAGCGCAGCAGCTTCAAGAGCTACGAGGGTCTGAGAGGTGGGAGATAATCAGCCATGTGTGGGTGGAGTTGCTTGCCAATATTTCATGTGAATGCGAATGGTACGAACATGCTAAGCAGCTTAGGCAGGGCGGGAGCCTGCTCACTCATGTTTGGCTTTTGATGCAACATCTTGGCTACATCAAGCAGGACAGCTTCTTCACCATGCAAGATCAAGAAGAAGAAGATACAGAACTAGTCGATGAAATCCTAAATAGTGATGTGGTGGGTCATATGTTGGATATTGTAGCTCGTGTGACAGGTCTCAATTCAAATTCCTAG

Coding sequence (CDS)

ATGGATACATCTGCTTTCTGGGATTTTATGTACGAATGGGAGCTTGTGTTTTTGCTCGTCGTAAGTTTATCTCTACAGTTGGTTCTGGTTCCAATGGGGTCGAAGAGAAAATACAGTTCCAACAACTGCCTCCGCCTGTTTCTCTTGATTGCATATTCTTCTGCTGATTGGATCGTCATGGTTTCTTTGGGCATCGTCATTGAAAAATACGGCGGCGGCTGCTACGATGAATTTTCAGATCCCATATACATCATTAGAGCCTTTTGGATGCCATTTTTACTATTCCACCTCGGTGGCTCCGACACCATCACTGCCTACTCCATTCAAGACAACGACCTCTGGCTAAGAGCTCTTTTTCGCTTCATCACTAACCTTCTCCTTTCGTTTTACATCTTTATGCTAGCCCTGAACCGACCTAAGGCCCTCAACTATATAGCCATTCCCGTGTTTGTTGCTGGGATTATCAAGTATGCTCAGAAGATTTGTGCTTTGAGATCTGCTTCTGGCCAACGCTTGCGAGATTTGTTGGTTTCACCTCCTCCTCCTTTCAGACCCAACACTCAAAATTTTGCATTTCCCATCAAAATCACTTCCACAGAGATGGACCAGTTGAATGTTTCTAATAATAACGACCCATATTTCAGCGATCTTCACATGCTACATCTTGCTTACTGTTTCTTCAAGAGAGACAAGCGGTTGTTTGTAGATTTGGATTTGAGCTCCTATCATCTTCAACAAAGCCTTTACTACTTCGAGAAATTTGATTCTAAAACTGCGTTCAAAGTCATTGAGCTCGAGCTCGGGTTTATATACGATTTCTTCTACACAAAAGCCTTTGTCATTCATTCTCGATGGGGTCGTGTTCTTCGCCTCACCAGCGTATCTTCCCTTGTCATCGCCATGGTCGCCTTCAGCTGCATGAGTGACTGGCAGGATTATCCTTTAAAGTATGTGAGATTGACATTTATCCTCTTCTCTGCAGCTTTGGGGCTTGAGATCTATTCACTTCTCCTATTTCTACACTCAGATTGGAACGTAATTTCTTTGACTCGAAGCTCTTACTCCTTGCCTCGTTTGATCTTTAAACTCATCTCTCATTGTGGATGGTCTGTTTGGAAGAGAAGATGGTCGAATTCCACTTCGCAGTACAATATAATATCTCGTTGCTTGAAAGAGAAAAACGACGGACGTTATTATTATCGCAAGTTCTTAAGATCAATTTCTTGCATCACCAAAATCACGGCTGCTAATTCACGATGCATCTCTAATGACCTTCAAACGCGGATCTTCGAACAGCTAAAGGAGAAGAAGTTGGAAGCCAATGAAGCCAACAAGAAGCTACCTGGCTGGATTTTAAGAAAGCACAACTGTTATAATCAGCTTGGTTGGAGCCTAAAATTAGATTTCGACGAAAGCATCCTCCTTTGGCACATCGCCACAGATATTTGCTATCATAATAATTCTGATGCAGCATCAATTGATTCTTCTCTTCTTGAAGACAGCGTATTGTTGTCTGATTTTTTGACCGACATTTTAGCACAACGTCCATGGTTGTTTCCAAATGGGATGAGTCAAATAAGGCTTAGCGCCACAAATGACGATGTCGTTGAATTTCTACAGAAGAAAAAGTTGGTAGAAATAGCTAGTGATGCTTGTAAGAGAATGTTGGATTTGGAATTGCAGATTCCTCACGAAGAAAAACGGCGACGCCACTCAATGTTCTTTGATGGTTGTCGGCTAGCGCAGCAGCTTCAAGAGCTACGAGGGTCTGAGAGGTGGGAGATAATCAGCCATGTGTGGGTGGAGTTGCTTGCCAATATTTCATGTGAATGCGAATGGTACGAACATGCTAAGCAGCTTAGGCAGGGCGGGAGCCTGCTCACTCATGTTTGGCTTTTGATGCAACATCTTGGCTACATCAAGCAGGACAGCTTCTTCACCATGCAAGATCAAGAAGAAGAAGATACAGAACTAGTCGATGAAATCCTAAATAGTGATGTGGTGGGTCATATGTTGGATATTGTAGCTCGTGTGACAGGTCTCAATTCAAATTCCTAG

Protein sequence

MDTSAFWDFMYEWELVFLLVVSLSLQLVLVPMGSKRKYSSNNCLRLFLLIAYSSADWIVMVSLGIVIEKYGGGCYDEFSDPIYIIRAFWMPFLLFHLGGSDTITAYSIQDNDLWLRALFRFITNLLLSFYIFMLALNRPKALNYIAIPVFVAGIIKYAQKICALRSASGQRLRDLLVSPPPPFRPNTQNFAFPIKITSTEMDQLNVSNNNDPYFSDLHMLHLAYCFFKRDKRLFVDLDLSSYHLQQSLYYFEKFDSKTAFKVIELELGFIYDFFYTKAFVIHSRWGRVLRLTSVSSLVIAMVAFSCMSDWQDYPLKYVRLTFILFSAALGLEIYSLLLFLHSDWNVISLTRSSYSLPRLIFKLISHCGWSVWKRRWSNSTSQYNIISRCLKEKNDGRYYYRKFLRSISCITKITAANSRCISNDLQTRIFEQLKEKKLEANEANKKLPGWILRKHNCYNQLGWSLKLDFDESILLWHIATDICYHNNSDAASIDSSLLEDSVLLSDFLTDILAQRPWLFPNGMSQIRLSATNDDVVEFLQKKKLVEIASDACKRMLDLELQIPHEEKRRRHSMFFDGCRLAQQLQELRGSERWEIISHVWVELLANISCECEWYEHAKQLRQGGSLLTHVWLLMQHLGYIKQDSFFTMQDQEEEDTELVDEILNSDVVGHMLDIVARVTGLNSNS
Homology
BLAST of Spg022739 vs. NCBI nr
Match: XP_022141971.1 (uncharacterized protein LOC111012216 [Momordica charantia])

HSP 1 Score: 685.6 bits (1768), Expect = 4.3e-193
Identity = 377/653 (57.73%), Postives = 469/653 (71.82%), Query Frame = 0

Query: 9   FMYEWE--LVFLL-VVSLSLQLVLVPMGSKRKYSSNNCLRLFLLIAYSSADWIVMVSLGI 68
           F+  W   L FLL + S +LQ++L+PMG +RKYSSNN LRL LL+ Y SADW    SLG 
Sbjct: 11  FLQSWSFGLTFLLPIFSFTLQVILIPMGLRRKYSSNNALRLLLLLFYLSADWAATTSLGT 70

Query: 69  VIEKYGGGCYDEFSDPIYIIRAFWMPFLLFHLGGSDTITAYSIQDNDLWLRALFRFITNL 128
           ++ K+ G   D F   ++I+     PF+L HLGGSDTITAYS++DNDLW R+ F F   +
Sbjct: 71  LV-KFYGSYEDAFFGRLFIL----APFMLLHLGGSDTITAYSMEDNDLWYRSFFGFFVQV 130

Query: 129 LLSFYIFMLALNRPKALNYIAIPVFVAGIIKYAQKICALRSASGQRLRDLLVSPPPPFRP 188
            ++FYI +LAL +P+ L+++ IP+FVAGIIKY ++I   RS S QRL DLL+S    F P
Sbjct: 131 GIAFYILLLAL-QPQHLDFLGIPIFVAGIIKYGERIWVFRSTSTQRLPDLLLS-TTRFSP 190

Query: 189 NTQNFAFPIKITSTEMDQLNVSN--NNDPYFSDLHMLHLAYCFFKRDKRLFVDLDLSSYH 248
              N A    +      Q+NV +    +P+FS LH+LH+AY FFK +K LFVDL L+SY 
Sbjct: 191 IQINAAKSKHLHLEFPIQINVDHEATQNPHFSHLHLLHIAYYFFKTNKFLFVDLTLTSYD 250

Query: 249 LQQSLYYFEKFDSKTAFKVIELELGFIYDFFYTKAFVIHSRWGRVLRLTSVSSLVIAMVA 308
           LQQSL+YF +FDS+ AFKVIELELGF+YDFFYTKA +IHSRWG +LRLT++ S+V+ +V 
Sbjct: 251 LQQSLHYFMQFDSREAFKVIELELGFMYDFFYTKASIIHSRWGPILRLTTLFSIVVVIVT 310

Query: 309 FSC--MSDWQDYPLKYVRLTFILFSAALGLEIYSLLLFLHSDWNVISLTRSSYSLPRLIF 368
           F         + PL  + LT IL   AL LEI S +LFL SDWNVI LT+SSYSL  L F
Sbjct: 311 FHIDHFDPGFNNPLTNI-LTLILLYGALSLEISSFILFLCSDWNVIRLTKSSYSLAHLTF 370

Query: 369 KLISHCGWSVWKRRWSNSTSQYNIISRCLKEKNDGRYYYRKFLRSISCITKITAANSRCI 428
           K IS CGWSV K RWSNS  QYN+IS CLKE   GRY   K+ R+ S I+KI  A SR I
Sbjct: 371 KAISRCGWSVKKYRWSNSVRQYNLISCCLKETKYGRYC--KYFRT-SYISKIMTA-SRNI 430

Query: 429 SNDLQTRIFEQLKEKKLEANEANKKLPGWILRKHNCYNQLGWSLKLDFDESILLWHIATD 488
           S++L+TRIF+QL + KLE NE N+KLPGWILRKHNCYNQLGWSL+LD D+SILLWHIAT+
Sbjct: 431 SDELKTRIFQQLTQ-KLEVNEENRKLPGWILRKHNCYNQLGWSLELDSDQSILLWHIATN 490

Query: 489 ICYHNNSDAASIDSSLLEDSVLLSDFLTDILAQRPWLFPNGMSQIRLSATNDDVVEFLQK 548
           ICYH + +  + + SLLED  LLSDFLT +L     LF +GMS+IR   T D  +EF+Q+
Sbjct: 491 ICYHRDKETEASNCSLLEDGTLLSDFLTYLLVYHHSLFLDGMSEIRFCETVDSAIEFMQQ 550

Query: 549 KKLVEIASDACKRMLDLELQIPHEEKRRRHSMFFDGCRLAQQLQELRGSERWEIISHVWV 608
           +K +E  SDACK MLDLE    +  K   +S+FF GCRLA++LQ L G ERWEII+HVWV
Sbjct: 551 RKSIETTSDACKSMLDLETSTVY--KDAGNSVFFGGCRLARELQGLEGCERWEIINHVWV 610

Query: 609 ELLANISCECEWYEHAKQLRQGGSLLTHVWLLMQHLGYIKQDSFFTMQDQEEE 655
           E+LANISCEC WYEHAK+LR GG+LLTHVWLLM HLGYIK  +  TMQDQ++E
Sbjct: 611 EMLANISCECRWYEHAKKLRHGGNLLTHVWLLMHHLGYIKPANLSTMQDQQDE 648

BLAST of Spg022739 vs. NCBI nr
Match: XP_004139148.1 (uncharacterized protein LOC101222078 [Cucumis sativus] >KGN66604.1 hypothetical protein Csa_007023 [Cucumis sativus])

HSP 1 Score: 619.8 bits (1597), Expect = 2.9e-173
Identity = 361/685 (52.70%), Postives = 458/685 (66.86%), Query Frame = 0

Query: 5   AFWDFMYEWELVFLLVVSLSLQLVLVPMGSKRKYSSNNCLRLFLLIAYSSADWIVMVSLG 64
           +FW+FMY WE    L +SL +QLVLVP+G KRK SS+  LR FLLIAY+ +DWI   S  
Sbjct: 7   SFWEFMYLWEPSAALFLSLCVQLVLVPLGLKRKCSSSQFLRFFLLIAYTFSDWIANFSFV 66

Query: 65  IVIEKYGGGCYDEFSDPIYIIRAFWMPFLLFHLGGSDTITAYSIQDNDLWLRALFRFITN 124
           +++E+YG GCYD+F+DP+Y+IRAF   FLL HLGGSDTITAYS++DN+LWLR L   +  
Sbjct: 67  MLVERYGTGCYDDFTDPMYMIRAFLAHFLLLHLGGSDTITAYSMEDNELWLRTLLSMLAI 126

Query: 125 LLLSFYIFMLALNRPKALNYIAIPVFVAGIIKYAQKICALRSASGQRLRDLL-VSPPPPF 184
           L  S YIF+ AL  P +LNYI+IPV +AG+IK ++KI ALRSAS +RLRD L VS P P 
Sbjct: 127 LAASIYIFLQAL-LPTSLNYISIPVIIAGVIKNSEKIWALRSASAERLRDFLAVSTPSP- 186

Query: 185 RPNTQNFAFPIKITSTEMDQLNVSNNNDPYFSDLHMLHLAYCFFKRDKRLFVDLDLSSYH 244
                                 ++ +N+    D  +L +AY FF RDKRLFV L  +SY 
Sbjct: 187 ----------------------ITTHNEEEVQDFEVLRIAYYFFIRDKRLFVGLGPTSYD 246

Query: 245 LQQS-LYYFEKFDSKTAFKVIELELGFIYDFFYTKAFVIHSRWGRVLRLTSVSSLVIAMV 304
           LQQ+ L Y+EKF+SK+AFK+IELELGF+YDFFYTKA + HS  GR+ RLT+ SSLVIA +
Sbjct: 247 LQQNRLSYYEKFESKSAFKIIELELGFMYDFFYTKASINHSLCGRLFRLTTFSSLVIAFL 306

Query: 305 AFSCMSDWQDYPLKYVRLTFILFSAALGLEIYSLLLFLHSDWNVISL--TRS-SYSLPRL 364
            + C+ D Q+YP  YV L F+LFS AL +EIYSL LFL SDWNVI L  T+S S  LPRL
Sbjct: 307 TY-CLIDKQEYPSTYVNLIFLLFSGALSIEIYSLFLFLFSDWNVIWLLTTQSPSNPLPRL 366

Query: 365 IFKLISHCGWSVWKRRWSNSTSQYNIISRCLKEKNDGRYYYRKFLRSISCITKITAANS- 424
             KLIS CGWS+ KRR SNS SQYN+IS CL++KND   YY KF       TK  AA S 
Sbjct: 367 ALKLISLCGWSLKKRRCSNSISQYNLISHCLEQKNDS--YYFKFPS-----TKTIAAFSV 426

Query: 425 -RCISNDLQTRIFEQLKEKKLEANEANKKLPGWILRKHNCYNQLGWSLKLDFDESILLWH 484
            R ISN+L+  IF+QLK+K +   E +             YN++GWSLKLD D+SIL+WH
Sbjct: 427 QRPISNNLEAHIFQQLKQKLVLNQEYDYG-----------YNEIGWSLKLDLDQSILIWH 486

Query: 485 IATDICYHNNSDAASIDSSLL----EDSVLLSDFLTDILAQRPWLFPNGMSQIRLSATND 544
           IATD CYH++      + S      +DSV LS+FL   +   P LFP+GMSQIR  AT++
Sbjct: 487 IATDFCYHSSPKFKESEESKSCIPPQDSVSLSNFLAYFIVHHPSLFPSGMSQIRHKATSE 546

Query: 545 DVVEFLQKKKLVEIASDACKRMLDLELQIPHEEKRRRHSMFFDGCRLAQQLQELRGSERW 604
            V+E LQ +KL    S+  K   +LEL I   ++ R+ S   D  RLA  L++L  S++W
Sbjct: 547 HVLELLQDEKLDRCRSNMLK---NLELNIEVVKEERKESRVLDAFRLAGFLEKLEQSQKW 606

Query: 605 EIISHVWVELLANISCECEWYEHAKQLRQGGSLLTHVWLLMQHLGYIKQDSFFT---MQD 664
           EII +VWVELL  ISCECEWY+HAKQL QGGSL+T VW+LM HLGY+KQ+  FT    +D
Sbjct: 607 EIIGNVWVELLGRISCECEWYDHAKQLTQGGSLVTRVWILMHHLGYLKQNDVFTTHIQED 645

Query: 665 QEEEDTELVDEILNSDVVGHMLDIV 676
           Q++    L  EI+   VV  ML++V
Sbjct: 667 QQDTTPLLGHEIVADYVVEQMLNVV 645

BLAST of Spg022739 vs. NCBI nr
Match: KAA0037446.1 (uncharacterized protein E6C27_scaffold277G00320 [Cucumis melo var. makuwa] >TYK01920.1 uncharacterized protein E5676_scaffold808G00060 [Cucumis melo var. makuwa])

HSP 1 Score: 619.4 bits (1596), Expect = 3.7e-173
Identity = 365/686 (53.21%), Postives = 456/686 (66.47%), Query Frame = 0

Query: 5   AFWDFMYEWELVFLLVVSLSLQLVLVPMGSKRKYSSNNCLRLFLLIAYSSADWIVMVSLG 64
           +FWDFMY WE    L +SL +QLVLVPMG KRK+SS+  LR FLLIAYS +DWI   S  
Sbjct: 7   SFWDFMYLWEPSAALFLSLCVQLVLVPMGLKRKHSSSQFLRFFLLIAYSFSDWIANFSFV 66

Query: 65  IVIEKYGGGCYDEFSDPIYIIRAFWMPFLLFHLGGSDTITAYSIQDNDLWLRALFRFITN 124
           +++E+YG GCYD+F+DP YIIRAF   FLL HLGGSDTITAYS++DN+LWLR L   +  
Sbjct: 67  MLVERYGTGCYDDFTDPNYIIRAFLAHFLLLHLGGSDTITAYSMEDNELWLRTLLSLLAV 126

Query: 125 LLLSFYIFMLALNRPKALNYIAIPVFVAGIIKYAQKICALRSASGQRLRDLL-VSPPPPF 184
           L  S YIF+ AL  P +LNY++IPV +AGIIK  +KI ALRSAS +RLRD L VS P P 
Sbjct: 127 LAASIYIFLQAL-LPTSLNYVSIPVIIAGIIKNCEKIWALRSASAERLRDFLAVSTPSP- 186

Query: 185 RPNTQNFAFPIKITSTEMDQLNVSNNNDPYFSDLHMLHLAYCFFKRDKRLFVDLDLSSYH 244
                                 ++ +N+    D  MLH+AY FF RDKRLFV L  +SY 
Sbjct: 187 ----------------------ITTHNEEEVQDFEMLHIAYYFFNRDKRLFVGLGPTSYD 246

Query: 245 LQQS-LYYFEKFDSKTAFKVIELELGFIYDFFYTKAFVIHSRWGRVLRLTSVSSLVIAMV 304
           LQQ+ L Y+EKF S +AFK+IELELGF+YDFFYTKA + HS  GR+ RLT+ SSL IA++
Sbjct: 247 LQQNRLSYYEKFQSNSAFKIIELELGFMYDFFYTKASINHSLCGRLFRLTTFSSLFIAIL 306

Query: 305 AFSCMSDWQDYPLKYVRLTFILFSAALGLEIYSLLLFLHSDWNVISL--TRS-SYSLPRL 364
            + C+ D Q+YP  YV L F+LF  AL +EIYSL L L SDWNVI L  T+S S  LPRL
Sbjct: 307 TY-CLIDKQEYPSTYVNLIFLLFFGALSIEIYSLFLILFSDWNVIWLLTTQSPSNPLPRL 366

Query: 365 IFKLISHCGWSVWKRRWSNSTSQYNIISRCLKEKNDGRYYYRKFLRSISCITKITAANS- 424
             KLIS CGWS  KRR SNS SQYN+IS CLK+KND  YY  KF       TK  AA S 
Sbjct: 367 ALKLISLCGWSFKKRRCSNSISQYNLISHCLKQKNDDSYYC-KFHN-----TKTMAAFSV 426

Query: 425 -RCISNDLQTRIFEQLKEKKLEANEANKKLPGWILRKHNCYNQLGWSLKLDFDESILLWH 484
            R ISN+L+  IF+QLK KKL  N+             + YN++GWSLKLD D+SILLWH
Sbjct: 427 QRPISNNLEAHIFQQLK-KKLVLNQ----------EYDSGYNEIGWSLKLDLDQSILLWH 486

Query: 485 IATDICYHNN-----SDAASIDSSLLEDSVLLSDFLTDILAQRPWLFPNGMSQIRLSATN 544
           IATD CY+++     S+  S      +DS+ LS+FL   +   P LFP+GMSQIR  AT+
Sbjct: 487 IATDFCYYSSPKFKESEEYSESCIPPQDSISLSNFLAYFIVHHPSLFPSGMSQIRHKATS 546

Query: 545 DDVVEFLQKKKLVEIASDACKRMLDLELQIPHEEKRRRHSMFFDGCRLAQQLQELRGSER 604
           +DV+E LQ KKL    S+  K   +LEL+I   ++ R+ SM  D CRLA  L++L  S++
Sbjct: 547 EDVLELLQDKKLGRCNSNMLK---NLELKIEVVKEERKESMVLDACRLAGILEKLEQSQK 606

Query: 605 WEIISHVWVELLANISCECEWYEHAKQLRQGGSLLTHVWLLMQHLGYIKQDSFFTM---Q 664
           WEII +VWVELL  ISCE EWY+HAKQL QGG+L+T VW+LM HLG  K +  FTM   +
Sbjct: 607 WEIIGNVWVELLGRISCEFEWYDHAKQLTQGGNLVTRVWILMHHLGCDKPNDVFTMHMQE 647

Query: 665 DQEEEDTELVDEILNSDVVGHMLDIV 676
           DQ++    L  +I+   VV  ML+++
Sbjct: 667 DQQDTTPLLGHDIVADYVVEQMLNVI 647

BLAST of Spg022739 vs. NCBI nr
Match: XP_008458716.1 (PREDICTED: uncharacterized protein LOC103498043 [Cucumis melo])

HSP 1 Score: 612.1 bits (1577), Expect = 6.0e-171
Identity = 361/686 (52.62%), Postives = 453/686 (66.03%), Query Frame = 0

Query: 5   AFWDFMYEWELVFLLVVSLSLQLVLVPMGSKRKYSSNNCLRLFLLIAYSSADWIVMVSLG 64
           +FWDFMY WE    L +SL +QLVLVPMG KRK+SS+   R FLLIAYS +DWI   S  
Sbjct: 7   SFWDFMYLWEPSAALFLSLCVQLVLVPMGLKRKHSSSQFRRFFLLIAYSFSDWIANFSFV 66

Query: 65  IVIEKYGGGCYDEFSDPIYIIRAFWMPFLLFHLGGSDTITAYSIQDNDLWLRALFRFITN 124
           +++E+YG GCYD+F+DP YIIRAF   FLL HLGG DTITAYS++DN+LWLR L   +  
Sbjct: 67  MLVERYGTGCYDDFTDPNYIIRAFLAHFLLLHLGGFDTITAYSMEDNELWLRTLLSLLAV 126

Query: 125 LLLSFYIFMLALNRPKALNYIAIPVFVAGIIKYAQKICALRSASGQRLRDLL-VSPPPPF 184
           L  S YIF+ AL  P +LNY++IPV +AGIIK  +KI ALRSAS +RLRD L VS P P 
Sbjct: 127 LAASIYIFLQAL-LPTSLNYVSIPVIIAGIIKNCEKIWALRSASAERLRDFLAVSTPSP- 186

Query: 185 RPNTQNFAFPIKITSTEMDQLNVSNNNDPYFSDLHMLHLAYCFFKRDKRLFVDLDLSSYH 244
                                 ++ +N+    D  MLH+AY FF RDKRLFV L  +SY 
Sbjct: 187 ----------------------ITTHNEEEVQDFEMLHIAYYFFNRDKRLFVGLGPTSYD 246

Query: 245 LQQS-LYYFEKFDSKTAFKVIELELGFIYDFFYTKAFVIHSRWGRVLRLTSVSSLVIAMV 304
           LQQ+ L Y+EKF S +AFK+IELELGF+YDFFYTKA + HS  GR+ RLT+ SSL IA++
Sbjct: 247 LQQNRLCYYEKFQSNSAFKIIELELGFMYDFFYTKASINHSLCGRLFRLTTFSSLFIAIL 306

Query: 305 AFSCMSDWQDYPLKYVRLTFILFSAALGLEIYSLLLFLHSDWNVISL--TRS-SYSLPRL 364
            + C+ D Q+YP  YV L F+LF  AL +EIYSL L L SDWNVI L  T+S S  LPRL
Sbjct: 307 TY-CLIDKQEYPSTYVNLIFLLFFGALSIEIYSLFLILFSDWNVIWLLTTQSPSNPLPRL 366

Query: 365 IFKLISHCGWSVWKRRWSNSTSQYNIISRCLKEKNDGRYYYRKFLRSISCITKITAANS- 424
             KLIS CGWS  KRR SNS SQYN+IS CLK+KND  YY  KF       TK  AA S 
Sbjct: 367 ALKLISLCGWSFKKRRCSNSISQYNLISHCLKQKNDDSYYC-KFHN-----TKTMAAFSV 426

Query: 425 -RCISNDLQTRIFEQLKEKKLEANEANKKLPGWILRKHNCYNQLGWSLKLDFDESILLWH 484
            R ISN+L+  IF+QLK KKL  N+             + YN++GWSLKLD D+SILLWH
Sbjct: 427 QRPISNNLEAHIFQQLK-KKLVLNQ----------EYDSGYNEIGWSLKLDLDQSILLWH 486

Query: 485 IATDICYHNN-----SDAASIDSSLLEDSVLLSDFLTDILAQRPWLFPNGMSQIRLSATN 544
           IATD CY+++     S+  S      +DS+ LS+FL   +   P LFP+ MSQIR  AT+
Sbjct: 487 IATDFCYYSSPKFKESEEYSESCIPPQDSISLSNFLAYFIVHHPSLFPSRMSQIRHKATS 546

Query: 545 DDVVEFLQKKKLVEIASDACKRMLDLELQIPHEEKRRRHSMFFDGCRLAQQLQELRGSER 604
           +DV+E LQ KKL    S+  K   +LEL+I   ++ R+ SM  D CRLA  L++L  S++
Sbjct: 547 EDVLELLQDKKLGRCNSNMLK---NLELKIEVVKEERKESMVLDACRLAGILEKLEQSQK 606

Query: 605 WEIISHVWVELLANISCECEWYEHAKQLRQGGSLLTHVWLLMQHLGYIKQDSFFTM---Q 664
           WEII +VWVELL  ISCE EWY+HAK L QGG+L+T VW+LM HLG +K +  FTM   +
Sbjct: 607 WEIIGNVWVELLGRISCEFEWYDHAKHLTQGGNLVTRVWILMHHLGCVKPNDVFTMHMQE 647

Query: 665 DQEEEDTELVDEILNSDVVGHMLDIV 676
           DQ++    L  +I+   VV  ML+++
Sbjct: 667 DQQDTTPLLGHDIVADYVVEQMLNVI 647

BLAST of Spg022739 vs. NCBI nr
Match: GFZ17712.1 (transmembrane protein, putative [Actinidia rufa])

HSP 1 Score: 474.9 bits (1221), Expect = 1.1e-129
Identity = 280/673 (41.60%), Postives = 400/673 (59.44%), Query Frame = 0

Query: 12  EWELVFLLVVSLSLQLVLVPMGSKRKYSSNNCLRLFLLIAYSSADWIVMVSLGIVIEKYG 71
           +WEL   +V+SL LQ++L+ +GS+RK ++ N +R+ L +AY  ADW+  VSLG++    G
Sbjct: 14  KWELRATVVLSLFLQIILIGLGSRRKSNTKNWIRIILWLAYLLADWVATVSLGVLANNQG 73

Query: 72  -GGCYDEFSDPIYIIRAFWMPFLLFHLGGSDTITAYSIQDNDLWLRALFRFITNLLLSFY 131
                D  S   Y+I AFW PFLL HLGG DTITAYS++DN+LWLR L   +  + ++ Y
Sbjct: 74  DSNSKDHNSPDSYVIMAFWAPFLLLHLGGPDTITAYSLEDNELWLRHLLGLVVQVGVALY 133

Query: 132 IFMLALNRPKALNYIAIPVFVAGIIKYAQKICALRSASGQRLRDLLVSPPPPFRPNTQNF 191
           + + AL RP  LN+IAIP+F+AGI+KY ++   LRSAS Q  R  L+  P P  PN   F
Sbjct: 134 VSVRAL-RPADLNFIAIPMFIAGIVKYGERTWILRSASSQHFRKSLLPRPDP-GPNYSKF 193

Query: 192 AFPIKITSTEMDQLNVSNNNDP-------------YFSDLHMLHLAYCFFKRDKRLFVDL 251
                +  TE  +L+ S    P                D  +L+ A+ FF   KRLF DL
Sbjct: 194 MEEYMLKETEGFELSWSVTQAPKVVAPDYAAGTNRLIPDAKILNAAHDFFLTFKRLFADL 253

Query: 252 DLSSYHLQQSLYYFEKFDSKTAFKVIELELGFIYDFFYTKAFVIHSRWGRVLRLTSVSSL 311
            LS   L++S  +F++   K AF+VI +ELGF+YD  YTKA VI+S WG +LR TS+SS 
Sbjct: 254 ILSFQDLEKSASFFQENSWKDAFEVIGVELGFVYDVLYTKAAVIYSIWGGILRCTSLSST 313

Query: 312 VIAMVAFSCMSDWQDYPLKYVRLTFILFSAALGLEIYSLLLFLHSDWNVISLTRSSYSLP 371
           VIA +AF C  DW  Y    V +TF+L   A+GLEIY++LL L SDW +  L +    L 
Sbjct: 314 VIAFMAF-CAIDWHGYSPVDVSITFLLLVGAIGLEIYAILLLLSSDWTLFWLIKHDNKLV 373

Query: 372 RLIFKLISHCGWSVWKRRWSNSTSQYNIISRCLKEKNDGRYY-YRKFLRSISCITKITAA 431
            L++ +IS     + K++WSN  +QYN+++ C K+K         K++     + K    
Sbjct: 374 YLVYTVISSFKCIISKKKWSNCMAQYNLLNSCFKDKKTSTCCDILKWMGMNEILEKWPWE 433

Query: 432 NSRCISNDLQTRIFEQLKEKKLEANEAN--KKL----PGWILRKHNCYNQLGWSLKLDFD 491
            S+ + + L+  IF QL EK   A++    K+L       +L+  NC N LGWS++++FD
Sbjct: 434 TSKNVPSKLKESIFAQLVEKSKSASDFRVCKQLCSARGDRVLQNWNCLNDLGWSVEVEFD 493

Query: 492 ESILLWHIATDICYHNNSD-----------AASIDSSL--LEDSVLLSDFLTDILAQRPW 551
            SILLWHIATD+CYH++ D              +DS     E S LLSD++  IL +RP 
Sbjct: 494 HSILLWHIATDLCYHSDKDNHPDKDHHSVKDRDLDSVFDPCEMSKLLSDYMLYILVKRPN 553

Query: 552 LFPNGMSQIRLSATNDDVVEFLQKKKLVEIASDACKRMLDLELQIPHEEKR--RRHSMFF 611
           + PNG+ QIR   T  +V+EF Q K  +     ACK++L ++++IP  E +  R  S+ F
Sbjct: 554 MLPNGIGQIRFQDTCAEVLEFSQDKNCMSDERLACKKLLRVDVKIPPAEVKGDRSKSLLF 613

Query: 612 DGCRLAQQLQELR------GSERWEIISHVWVELLANISCECEWYEHAKQLRQGGSLLTH 643
           D C+LA+ LQ L+        ++WE+I HVW+E+L+  + +C W +HA+QLR+GG LLTH
Sbjct: 614 DACKLAKALQSLQIEREWEMQQKWEMICHVWLEILSYAASQCMWNQHAQQLRRGGELLTH 673

BLAST of Spg022739 vs. ExPASy TrEMBL
Match: A0A6J1CKT2 (uncharacterized protein LOC111012216 OS=Momordica charantia OX=3673 GN=LOC111012216 PE=4 SV=1)

HSP 1 Score: 685.6 bits (1768), Expect = 2.1e-193
Identity = 377/653 (57.73%), Postives = 469/653 (71.82%), Query Frame = 0

Query: 9   FMYEWE--LVFLL-VVSLSLQLVLVPMGSKRKYSSNNCLRLFLLIAYSSADWIVMVSLGI 68
           F+  W   L FLL + S +LQ++L+PMG +RKYSSNN LRL LL+ Y SADW    SLG 
Sbjct: 11  FLQSWSFGLTFLLPIFSFTLQVILIPMGLRRKYSSNNALRLLLLLFYLSADWAATTSLGT 70

Query: 69  VIEKYGGGCYDEFSDPIYIIRAFWMPFLLFHLGGSDTITAYSIQDNDLWLRALFRFITNL 128
           ++ K+ G   D F   ++I+     PF+L HLGGSDTITAYS++DNDLW R+ F F   +
Sbjct: 71  LV-KFYGSYEDAFFGRLFIL----APFMLLHLGGSDTITAYSMEDNDLWYRSFFGFFVQV 130

Query: 129 LLSFYIFMLALNRPKALNYIAIPVFVAGIIKYAQKICALRSASGQRLRDLLVSPPPPFRP 188
            ++FYI +LAL +P+ L+++ IP+FVAGIIKY ++I   RS S QRL DLL+S    F P
Sbjct: 131 GIAFYILLLAL-QPQHLDFLGIPIFVAGIIKYGERIWVFRSTSTQRLPDLLLS-TTRFSP 190

Query: 189 NTQNFAFPIKITSTEMDQLNVSN--NNDPYFSDLHMLHLAYCFFKRDKRLFVDLDLSSYH 248
              N A    +      Q+NV +    +P+FS LH+LH+AY FFK +K LFVDL L+SY 
Sbjct: 191 IQINAAKSKHLHLEFPIQINVDHEATQNPHFSHLHLLHIAYYFFKTNKFLFVDLTLTSYD 250

Query: 249 LQQSLYYFEKFDSKTAFKVIELELGFIYDFFYTKAFVIHSRWGRVLRLTSVSSLVIAMVA 308
           LQQSL+YF +FDS+ AFKVIELELGF+YDFFYTKA +IHSRWG +LRLT++ S+V+ +V 
Sbjct: 251 LQQSLHYFMQFDSREAFKVIELELGFMYDFFYTKASIIHSRWGPILRLTTLFSIVVVIVT 310

Query: 309 FSC--MSDWQDYPLKYVRLTFILFSAALGLEIYSLLLFLHSDWNVISLTRSSYSLPRLIF 368
           F         + PL  + LT IL   AL LEI S +LFL SDWNVI LT+SSYSL  L F
Sbjct: 311 FHIDHFDPGFNNPLTNI-LTLILLYGALSLEISSFILFLCSDWNVIRLTKSSYSLAHLTF 370

Query: 369 KLISHCGWSVWKRRWSNSTSQYNIISRCLKEKNDGRYYYRKFLRSISCITKITAANSRCI 428
           K IS CGWSV K RWSNS  QYN+IS CLKE   GRY   K+ R+ S I+KI  A SR I
Sbjct: 371 KAISRCGWSVKKYRWSNSVRQYNLISCCLKETKYGRYC--KYFRT-SYISKIMTA-SRNI 430

Query: 429 SNDLQTRIFEQLKEKKLEANEANKKLPGWILRKHNCYNQLGWSLKLDFDESILLWHIATD 488
           S++L+TRIF+QL + KLE NE N+KLPGWILRKHNCYNQLGWSL+LD D+SILLWHIAT+
Sbjct: 431 SDELKTRIFQQLTQ-KLEVNEENRKLPGWILRKHNCYNQLGWSLELDSDQSILLWHIATN 490

Query: 489 ICYHNNSDAASIDSSLLEDSVLLSDFLTDILAQRPWLFPNGMSQIRLSATNDDVVEFLQK 548
           ICYH + +  + + SLLED  LLSDFLT +L     LF +GMS+IR   T D  +EF+Q+
Sbjct: 491 ICYHRDKETEASNCSLLEDGTLLSDFLTYLLVYHHSLFLDGMSEIRFCETVDSAIEFMQQ 550

Query: 549 KKLVEIASDACKRMLDLELQIPHEEKRRRHSMFFDGCRLAQQLQELRGSERWEIISHVWV 608
           +K +E  SDACK MLDLE    +  K   +S+FF GCRLA++LQ L G ERWEII+HVWV
Sbjct: 551 RKSIETTSDACKSMLDLETSTVY--KDAGNSVFFGGCRLARELQGLEGCERWEIINHVWV 610

Query: 609 ELLANISCECEWYEHAKQLRQGGSLLTHVWLLMQHLGYIKQDSFFTMQDQEEE 655
           E+LANISCEC WYEHAK+LR GG+LLTHVWLLM HLGYIK  +  TMQDQ++E
Sbjct: 611 EMLANISCECRWYEHAKKLRHGGNLLTHVWLLMHHLGYIKPANLSTMQDQQDE 648

BLAST of Spg022739 vs. ExPASy TrEMBL
Match: A0A0A0LZZ2 (DUF4220 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G638510 PE=4 SV=1)

HSP 1 Score: 619.8 bits (1597), Expect = 1.4e-173
Identity = 361/685 (52.70%), Postives = 458/685 (66.86%), Query Frame = 0

Query: 5   AFWDFMYEWELVFLLVVSLSLQLVLVPMGSKRKYSSNNCLRLFLLIAYSSADWIVMVSLG 64
           +FW+FMY WE    L +SL +QLVLVP+G KRK SS+  LR FLLIAY+ +DWI   S  
Sbjct: 7   SFWEFMYLWEPSAALFLSLCVQLVLVPLGLKRKCSSSQFLRFFLLIAYTFSDWIANFSFV 66

Query: 65  IVIEKYGGGCYDEFSDPIYIIRAFWMPFLLFHLGGSDTITAYSIQDNDLWLRALFRFITN 124
           +++E+YG GCYD+F+DP+Y+IRAF   FLL HLGGSDTITAYS++DN+LWLR L   +  
Sbjct: 67  MLVERYGTGCYDDFTDPMYMIRAFLAHFLLLHLGGSDTITAYSMEDNELWLRTLLSMLAI 126

Query: 125 LLLSFYIFMLALNRPKALNYIAIPVFVAGIIKYAQKICALRSASGQRLRDLL-VSPPPPF 184
           L  S YIF+ AL  P +LNYI+IPV +AG+IK ++KI ALRSAS +RLRD L VS P P 
Sbjct: 127 LAASIYIFLQAL-LPTSLNYISIPVIIAGVIKNSEKIWALRSASAERLRDFLAVSTPSP- 186

Query: 185 RPNTQNFAFPIKITSTEMDQLNVSNNNDPYFSDLHMLHLAYCFFKRDKRLFVDLDLSSYH 244
                                 ++ +N+    D  +L +AY FF RDKRLFV L  +SY 
Sbjct: 187 ----------------------ITTHNEEEVQDFEVLRIAYYFFIRDKRLFVGLGPTSYD 246

Query: 245 LQQS-LYYFEKFDSKTAFKVIELELGFIYDFFYTKAFVIHSRWGRVLRLTSVSSLVIAMV 304
           LQQ+ L Y+EKF+SK+AFK+IELELGF+YDFFYTKA + HS  GR+ RLT+ SSLVIA +
Sbjct: 247 LQQNRLSYYEKFESKSAFKIIELELGFMYDFFYTKASINHSLCGRLFRLTTFSSLVIAFL 306

Query: 305 AFSCMSDWQDYPLKYVRLTFILFSAALGLEIYSLLLFLHSDWNVISL--TRS-SYSLPRL 364
            + C+ D Q+YP  YV L F+LFS AL +EIYSL LFL SDWNVI L  T+S S  LPRL
Sbjct: 307 TY-CLIDKQEYPSTYVNLIFLLFSGALSIEIYSLFLFLFSDWNVIWLLTTQSPSNPLPRL 366

Query: 365 IFKLISHCGWSVWKRRWSNSTSQYNIISRCLKEKNDGRYYYRKFLRSISCITKITAANS- 424
             KLIS CGWS+ KRR SNS SQYN+IS CL++KND   YY KF       TK  AA S 
Sbjct: 367 ALKLISLCGWSLKKRRCSNSISQYNLISHCLEQKNDS--YYFKFPS-----TKTIAAFSV 426

Query: 425 -RCISNDLQTRIFEQLKEKKLEANEANKKLPGWILRKHNCYNQLGWSLKLDFDESILLWH 484
            R ISN+L+  IF+QLK+K +   E +             YN++GWSLKLD D+SIL+WH
Sbjct: 427 QRPISNNLEAHIFQQLKQKLVLNQEYDYG-----------YNEIGWSLKLDLDQSILIWH 486

Query: 485 IATDICYHNNSDAASIDSSLL----EDSVLLSDFLTDILAQRPWLFPNGMSQIRLSATND 544
           IATD CYH++      + S      +DSV LS+FL   +   P LFP+GMSQIR  AT++
Sbjct: 487 IATDFCYHSSPKFKESEESKSCIPPQDSVSLSNFLAYFIVHHPSLFPSGMSQIRHKATSE 546

Query: 545 DVVEFLQKKKLVEIASDACKRMLDLELQIPHEEKRRRHSMFFDGCRLAQQLQELRGSERW 604
            V+E LQ +KL    S+  K   +LEL I   ++ R+ S   D  RLA  L++L  S++W
Sbjct: 547 HVLELLQDEKLDRCRSNMLK---NLELNIEVVKEERKESRVLDAFRLAGFLEKLEQSQKW 606

Query: 605 EIISHVWVELLANISCECEWYEHAKQLRQGGSLLTHVWLLMQHLGYIKQDSFFT---MQD 664
           EII +VWVELL  ISCECEWY+HAKQL QGGSL+T VW+LM HLGY+KQ+  FT    +D
Sbjct: 607 EIIGNVWVELLGRISCECEWYDHAKQLTQGGSLVTRVWILMHHLGYLKQNDVFTTHIQED 645

Query: 665 QEEEDTELVDEILNSDVVGHMLDIV 676
           Q++    L  EI+   VV  ML++V
Sbjct: 667 QQDTTPLLGHEIVADYVVEQMLNVV 645

BLAST of Spg022739 vs. ExPASy TrEMBL
Match: A0A5D3BS41 (DUF4220 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G00060 PE=4 SV=1)

HSP 1 Score: 619.4 bits (1596), Expect = 1.8e-173
Identity = 365/686 (53.21%), Postives = 456/686 (66.47%), Query Frame = 0

Query: 5   AFWDFMYEWELVFLLVVSLSLQLVLVPMGSKRKYSSNNCLRLFLLIAYSSADWIVMVSLG 64
           +FWDFMY WE    L +SL +QLVLVPMG KRK+SS+  LR FLLIAYS +DWI   S  
Sbjct: 7   SFWDFMYLWEPSAALFLSLCVQLVLVPMGLKRKHSSSQFLRFFLLIAYSFSDWIANFSFV 66

Query: 65  IVIEKYGGGCYDEFSDPIYIIRAFWMPFLLFHLGGSDTITAYSIQDNDLWLRALFRFITN 124
           +++E+YG GCYD+F+DP YIIRAF   FLL HLGGSDTITAYS++DN+LWLR L   +  
Sbjct: 67  MLVERYGTGCYDDFTDPNYIIRAFLAHFLLLHLGGSDTITAYSMEDNELWLRTLLSLLAV 126

Query: 125 LLLSFYIFMLALNRPKALNYIAIPVFVAGIIKYAQKICALRSASGQRLRDLL-VSPPPPF 184
           L  S YIF+ AL  P +LNY++IPV +AGIIK  +KI ALRSAS +RLRD L VS P P 
Sbjct: 127 LAASIYIFLQAL-LPTSLNYVSIPVIIAGIIKNCEKIWALRSASAERLRDFLAVSTPSP- 186

Query: 185 RPNTQNFAFPIKITSTEMDQLNVSNNNDPYFSDLHMLHLAYCFFKRDKRLFVDLDLSSYH 244
                                 ++ +N+    D  MLH+AY FF RDKRLFV L  +SY 
Sbjct: 187 ----------------------ITTHNEEEVQDFEMLHIAYYFFNRDKRLFVGLGPTSYD 246

Query: 245 LQQS-LYYFEKFDSKTAFKVIELELGFIYDFFYTKAFVIHSRWGRVLRLTSVSSLVIAMV 304
           LQQ+ L Y+EKF S +AFK+IELELGF+YDFFYTKA + HS  GR+ RLT+ SSL IA++
Sbjct: 247 LQQNRLSYYEKFQSNSAFKIIELELGFMYDFFYTKASINHSLCGRLFRLTTFSSLFIAIL 306

Query: 305 AFSCMSDWQDYPLKYVRLTFILFSAALGLEIYSLLLFLHSDWNVISL--TRS-SYSLPRL 364
            + C+ D Q+YP  YV L F+LF  AL +EIYSL L L SDWNVI L  T+S S  LPRL
Sbjct: 307 TY-CLIDKQEYPSTYVNLIFLLFFGALSIEIYSLFLILFSDWNVIWLLTTQSPSNPLPRL 366

Query: 365 IFKLISHCGWSVWKRRWSNSTSQYNIISRCLKEKNDGRYYYRKFLRSISCITKITAANS- 424
             KLIS CGWS  KRR SNS SQYN+IS CLK+KND  YY  KF       TK  AA S 
Sbjct: 367 ALKLISLCGWSFKKRRCSNSISQYNLISHCLKQKNDDSYYC-KFHN-----TKTMAAFSV 426

Query: 425 -RCISNDLQTRIFEQLKEKKLEANEANKKLPGWILRKHNCYNQLGWSLKLDFDESILLWH 484
            R ISN+L+  IF+QLK KKL  N+             + YN++GWSLKLD D+SILLWH
Sbjct: 427 QRPISNNLEAHIFQQLK-KKLVLNQ----------EYDSGYNEIGWSLKLDLDQSILLWH 486

Query: 485 IATDICYHNN-----SDAASIDSSLLEDSVLLSDFLTDILAQRPWLFPNGMSQIRLSATN 544
           IATD CY+++     S+  S      +DS+ LS+FL   +   P LFP+GMSQIR  AT+
Sbjct: 487 IATDFCYYSSPKFKESEEYSESCIPPQDSISLSNFLAYFIVHHPSLFPSGMSQIRHKATS 546

Query: 545 DDVVEFLQKKKLVEIASDACKRMLDLELQIPHEEKRRRHSMFFDGCRLAQQLQELRGSER 604
           +DV+E LQ KKL    S+  K   +LEL+I   ++ R+ SM  D CRLA  L++L  S++
Sbjct: 547 EDVLELLQDKKLGRCNSNMLK---NLELKIEVVKEERKESMVLDACRLAGILEKLEQSQK 606

Query: 605 WEIISHVWVELLANISCECEWYEHAKQLRQGGSLLTHVWLLMQHLGYIKQDSFFTM---Q 664
           WEII +VWVELL  ISCE EWY+HAKQL QGG+L+T VW+LM HLG  K +  FTM   +
Sbjct: 607 WEIIGNVWVELLGRISCEFEWYDHAKQLTQGGNLVTRVWILMHHLGCDKPNDVFTMHMQE 647

Query: 665 DQEEEDTELVDEILNSDVVGHMLDIV 676
           DQ++    L  +I+   VV  ML+++
Sbjct: 667 DQQDTTPLLGHDIVADYVVEQMLNVI 647

BLAST of Spg022739 vs. ExPASy TrEMBL
Match: A0A1S3C8L7 (uncharacterized protein LOC103498043 OS=Cucumis melo OX=3656 GN=LOC103498043 PE=4 SV=1)

HSP 1 Score: 612.1 bits (1577), Expect = 2.9e-171
Identity = 361/686 (52.62%), Postives = 453/686 (66.03%), Query Frame = 0

Query: 5   AFWDFMYEWELVFLLVVSLSLQLVLVPMGSKRKYSSNNCLRLFLLIAYSSADWIVMVSLG 64
           +FWDFMY WE    L +SL +QLVLVPMG KRK+SS+   R FLLIAYS +DWI   S  
Sbjct: 7   SFWDFMYLWEPSAALFLSLCVQLVLVPMGLKRKHSSSQFRRFFLLIAYSFSDWIANFSFV 66

Query: 65  IVIEKYGGGCYDEFSDPIYIIRAFWMPFLLFHLGGSDTITAYSIQDNDLWLRALFRFITN 124
           +++E+YG GCYD+F+DP YIIRAF   FLL HLGG DTITAYS++DN+LWLR L   +  
Sbjct: 67  MLVERYGTGCYDDFTDPNYIIRAFLAHFLLLHLGGFDTITAYSMEDNELWLRTLLSLLAV 126

Query: 125 LLLSFYIFMLALNRPKALNYIAIPVFVAGIIKYAQKICALRSASGQRLRDLL-VSPPPPF 184
           L  S YIF+ AL  P +LNY++IPV +AGIIK  +KI ALRSAS +RLRD L VS P P 
Sbjct: 127 LAASIYIFLQAL-LPTSLNYVSIPVIIAGIIKNCEKIWALRSASAERLRDFLAVSTPSP- 186

Query: 185 RPNTQNFAFPIKITSTEMDQLNVSNNNDPYFSDLHMLHLAYCFFKRDKRLFVDLDLSSYH 244
                                 ++ +N+    D  MLH+AY FF RDKRLFV L  +SY 
Sbjct: 187 ----------------------ITTHNEEEVQDFEMLHIAYYFFNRDKRLFVGLGPTSYD 246

Query: 245 LQQS-LYYFEKFDSKTAFKVIELELGFIYDFFYTKAFVIHSRWGRVLRLTSVSSLVIAMV 304
           LQQ+ L Y+EKF S +AFK+IELELGF+YDFFYTKA + HS  GR+ RLT+ SSL IA++
Sbjct: 247 LQQNRLCYYEKFQSNSAFKIIELELGFMYDFFYTKASINHSLCGRLFRLTTFSSLFIAIL 306

Query: 305 AFSCMSDWQDYPLKYVRLTFILFSAALGLEIYSLLLFLHSDWNVISL--TRS-SYSLPRL 364
            + C+ D Q+YP  YV L F+LF  AL +EIYSL L L SDWNVI L  T+S S  LPRL
Sbjct: 307 TY-CLIDKQEYPSTYVNLIFLLFFGALSIEIYSLFLILFSDWNVIWLLTTQSPSNPLPRL 366

Query: 365 IFKLISHCGWSVWKRRWSNSTSQYNIISRCLKEKNDGRYYYRKFLRSISCITKITAANS- 424
             KLIS CGWS  KRR SNS SQYN+IS CLK+KND  YY  KF       TK  AA S 
Sbjct: 367 ALKLISLCGWSFKKRRCSNSISQYNLISHCLKQKNDDSYYC-KFHN-----TKTMAAFSV 426

Query: 425 -RCISNDLQTRIFEQLKEKKLEANEANKKLPGWILRKHNCYNQLGWSLKLDFDESILLWH 484
            R ISN+L+  IF+QLK KKL  N+             + YN++GWSLKLD D+SILLWH
Sbjct: 427 QRPISNNLEAHIFQQLK-KKLVLNQ----------EYDSGYNEIGWSLKLDLDQSILLWH 486

Query: 485 IATDICYHNN-----SDAASIDSSLLEDSVLLSDFLTDILAQRPWLFPNGMSQIRLSATN 544
           IATD CY+++     S+  S      +DS+ LS+FL   +   P LFP+ MSQIR  AT+
Sbjct: 487 IATDFCYYSSPKFKESEEYSESCIPPQDSISLSNFLAYFIVHHPSLFPSRMSQIRHKATS 546

Query: 545 DDVVEFLQKKKLVEIASDACKRMLDLELQIPHEEKRRRHSMFFDGCRLAQQLQELRGSER 604
           +DV+E LQ KKL    S+  K   +LEL+I   ++ R+ SM  D CRLA  L++L  S++
Sbjct: 547 EDVLELLQDKKLGRCNSNMLK---NLELKIEVVKEERKESMVLDACRLAGILEKLEQSQK 606

Query: 605 WEIISHVWVELLANISCECEWYEHAKQLRQGGSLLTHVWLLMQHLGYIKQDSFFTM---Q 664
           WEII +VWVELL  ISCE EWY+HAK L QGG+L+T VW+LM HLG +K +  FTM   +
Sbjct: 607 WEIIGNVWVELLGRISCEFEWYDHAKHLTQGGNLVTRVWILMHHLGCVKPNDVFTMHMQE 647

Query: 665 DQEEEDTELVDEILNSDVVGHMLDIV 676
           DQ++    L  +I+   VV  ML+++
Sbjct: 667 DQQDTTPLLGHDIVADYVVEQMLNVI 647

BLAST of Spg022739 vs. ExPASy TrEMBL
Match: A0A5B7BVN3 (DUF4220 domain-containing protein OS=Davidia involucrata OX=16924 GN=Din_042423 PE=4 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 6.5e-131
Identity = 274/661 (41.45%), Postives = 409/661 (61.88%), Query Frame = 0

Query: 13  WELVFLLVVSLSLQLVLVPMGSKRKYSSNNCLRLFLLIAYSSADWIVMVSLGIVIEKYGG 72
           WEL  ++++SLSLQ++L+ +G++RKY++   LR+ L ++Y SADW+  V+LG++    G 
Sbjct: 15  WELRAMVLLSLSLQIILIGLGNRRKYNTRIWLRIILWLSYLSADWVATVALGVLSNSQGD 74

Query: 73  GCYDEFSDPIYIIRAFWMPFLLFHLGGSDTITAYSIQDNDLWLRALFRFITNLLLSFYIF 132
               +   P Y+I AFW PFLL HLGG DTITAY+++DN+LWLR L   +  + ++ Y+F
Sbjct: 75  ---SDSEGPSYVIMAFWAPFLLVHLGGPDTITAYALEDNELWLRHLLGLVVQVGVALYVF 134

Query: 133 MLALNRPKALNYIAIPVFVAGIIKYAQKICALRSASGQRLRDLLVSPPPPFRPNTQNFAF 192
           + +LN P  LN++A+P+FVAGIIKY ++   LRSAS Q  R+ L+  P P  PN   F  
Sbjct: 135 VRSLN-PTDLNFVALPIFVAGIIKYGERTWVLRSASSQHFRESLLPRPDP-GPNFAKFME 194

Query: 193 PIKITSTEMDQLNVSNNNDP-----YFSDLHMLHLAYCFFKRDKRLFVDLDLSSYHLQQS 252
              +   E  +L+ +    P     Y++D   L+ AY FF   +RLF DL LS   L++S
Sbjct: 195 EYNLKDREGYKLSWTVTPAPTTTAHYYADATFLNAAYDFFLTYRRLFADLILSFQDLEKS 254

Query: 253 LYYFEKFDSKTAFKVIELELGFIYDFFYTKAFVIHSRWGRVLRLTSVSSLVIAMVAFSCM 312
           L +F++   + AF+VIE+ELGF+YD  YTKA +++S WG  LR T +SS +IA+V F C 
Sbjct: 255 LSFFQESSWENAFQVIEVELGFMYDILYTKATIVYSVWGVFLRFTCLSSTIIALVVF-CT 314

Query: 313 SDWQDYPLKYVRLTFILFSAALGLEIYSLLLFLHSDWNVISLTRSSYSLPRLIFKLISHC 372
            DW  Y    V ++F+L   A+GLEIY++LL L SDW ++  ++       LI K+IS  
Sbjct: 315 IDWHGYSQVDVGISFLLLLGAIGLEIYAILLLLSSDWTLLWFSKHDNLGVNLIKKVISPF 374

Query: 373 GWSVWKRRWSNSTSQYNIISRCLKEKNDGRYYYRKFLRSISCITKI----TAANSRCISN 432
                KR+WSNS +QYN++S C K K        K L+  +CI +I       +S  +S 
Sbjct: 375 NCVTSKRKWSNSMAQYNLLSSCFKYK---PAMCCKILK-CACIHRIIDDYLYESSEDVSP 434

Query: 433 DLQTRIFEQLKEKKLEANEAN--KKLPG----WILRKHNCYNQLGWSLKLDFDESILLWH 492
           +L+  IF+QL E    A+E    KKL       +L+KH+C  +LGWS+K +FD SILLWH
Sbjct: 435 NLKESIFKQLVENSKGASEFRDCKKLCACRGEQVLQKHDCLEKLGWSVKDEFDYSILLWH 494

Query: 493 IATDICYHN---NSDAASIDSSLLEDSVLLSDFLTDILAQRPWLFPNGMSQIRLSATNDD 552
           IATD+CY++   +  A  +  +  ++S LLS+++  IL +RP++ PNG+ QIR   T  +
Sbjct: 495 IATDLCYYSDYGDEGANFVPHAKCKESKLLSNYMLYILVKRPFMLPNGIGQIRFQDTCAE 554

Query: 553 VVEFLQKKKLVEIASD-----ACKRMLDLELQIPHEEKR--RRHSMFFDGCRLAQQLQEL 612
            +EF ++ +      D     ACK++L ++++IP  E +  R  S+ FD C+LA+ LQ L
Sbjct: 555 AMEFFEEDRTKFFQEDKTINLACKKLLQVDIKIPPAEVKGDRSKSVLFDACKLAKSLQSL 614

Query: 613 R------GSERWEIISHVWVELLANISCECEWYEHAKQLRQGGSLLTHVWLLMQHLGYIK 643
                    ++WE++SHVW+E+L+  + +C W  HA+QLR+GG LLTHVWLLM HLG  +
Sbjct: 615 ETQKQWGKQQKWEMVSHVWMEMLSYCASQCRWNHHAQQLRRGGELLTHVWLLMAHLGITE 665

BLAST of Spg022739 vs. TAIR 10
Match: AT5G45540.1 (Protein of unknown function (DUF594) )

HSP 1 Score: 231.5 bits (589), Expect = 2.0e-60
Identity = 203/778 (26.09%), Postives = 337/778 (43.32%), Query Frame = 0

Query: 7   WDFMYEWELVFLLVVSLSLQLVLVPMGSKRKYSSNNCLRLFLLIAYSSADWIVMVSLGIV 66
           WD   +W +  ++++SL LQ +L+     R+ ++     + +  AY  ADW    ++G +
Sbjct: 13  WD---KWNIRGVIILSLFLQTILIFFAPSRRRTAKKLFLVLIWSAYLLADWAADYAVGQI 72

Query: 67  IEKYGGGCYDEFSDPIYIIRAFWMPFLLFHLGGSDTITAYSIQDNDLWLRALFRFITNLL 126
            +                + AFW PFLL HLGG DTITA +++DN+LW R LF  +   +
Sbjct: 73  SDSQEEEAESNKPSKNRELLAFWSPFLLLHLGGPDTITALALEDNELWDRHLFSLVCQAV 132

Query: 127 LSFYIFMLALNRPKALNYIAIPVFVAGIIKYAQKICALRSASGQRLRDLLVSPPPPFR-- 186
            + Y+ +L++  P  L    + +FV G+IKY ++  AL SAS  + +D ++  P P    
Sbjct: 133 ATVYVILLSI--PNRLLTPTLIMFVGGVIKYVERTAALFSASLDKFKDSMLDDPDPGANY 192

Query: 187 -------PNTQNFAFPIKITSTEMDQLNVSNNN----DPYFSDLHMLHLAYCFFKRDKRL 246
                     +    P  +   +  +     N     D   + L ++  AY +F   K L
Sbjct: 193 AKLMEEYEARKKMNMPTDVIVVKDPEKGREGNTPVRPDNELTALQVIQYAYKYFNIFKGL 252

Query: 247 FVDLDLSSYHLQQSLYYFEKFDSKTAFKVIELELGFIYDFFYTKAFVIHSRWGRVLRLTS 306
            VDL  ++    +S  +F+K  ++ A ++IE+ELG IYD  +TKA ++H+  G V R  +
Sbjct: 253 IVDLIFTNQERDESRKFFDKLTAEEALRIIEVELGLIYDCLFTKAEILHNWTGAVFRFIA 312

Query: 307 VSSLVIAMVAFSCMSDWQDYPLKYVRLTFILFSAALGLEIYSLLLFLHSDWNVISLTRSS 366
           +  LV ++  F  M+    Y    V LT+ L    + L+  +LL+F  SDW +  L +  
Sbjct: 313 LGCLVASLCLFK-MNKKDQYDGFDVVLTYALLICGIALDSIALLMFCVSDWTIARLRKLK 372

Query: 367 YSLPR------------LIFKLI-------SHCGWSVWK-----RRWSNSTSQYNIISRC 426
             L              L FK +       S  G  V       RRWS     YN+I  C
Sbjct: 373 EDLEEKDTLTDRVLNWILDFKTLRWKRSKCSQDGHQVLNRNFMFRRWSEYVHAYNLIGFC 432

Query: 427 LKEKNDGRYYYR-----------------------------------KFLRSIS------ 486
           L  +    +Y +                                   +FL ++S      
Sbjct: 433 LGIRPKRIHYTKGKIHSFFHQTVHILSIDTAIENATRGTRQFHNWIGRFLSNLSKRDNSV 492

Query: 487 ------------------------------CITKITAANSRCISNDLQTRIFEQLKEKK- 546
                                          + +I    S  ++ +L   IF ++++K  
Sbjct: 493 IRTGLRWFLFFPQLLGLLIYNFLDFFGIKDLVEEIRFTVSDRLTRELWEFIFTEVQQKHR 552

Query: 547 -LEANEANKKLP----GWILRKHNCYNQ---------LGWSLKLDFDESILLWHIATDIC 606
             E  E+ K +      W L + +   +         L +  + D+D+SILLWHIAT++ 
Sbjct: 553 FAEDQESAKGISSARGNWTLLETSSKKKEDGTDHTKLLQYVTEKDYDQSILLWHIATELL 612

Query: 607 Y------------HNNSDAASIDSSLLEDSVLLSDFLTDILAQRPWLFP--NGMSQIRLS 639
           Y             ++++    + S  E S +LSD++  +L  +P L    +G+++IR  
Sbjct: 613 YQKPIDKKVTEKEEHSTNREKEEHSNREFSKILSDYMMYLLIVQPTLMSAVSGIAKIRFR 672

BLAST of Spg022739 vs. TAIR 10
Match: AT5G45470.1 (Protein of unknown function (DUF594) )

HSP 1 Score: 222.2 bits (565), Expect = 1.2e-57
Identity = 220/844 (26.07%), Postives = 346/844 (41.00%), Query Frame = 0

Query: 7   WDFMYEWELVFLLVVSLSLQLVLVPMGSKRKYSSNNCLRLFLLIAYSSADWIVMVSLGIV 66
           WD    W +   +++SL+LQ +L+     RK +    L + +  +Y  ADW    ++G++
Sbjct: 13  WD---RWNIRGAVILSLTLQAILICFSPLRKRTPRRLLIVLVWSSYLLADWSANFAVGLI 72

Query: 67  IEKYGGGCYDEFSDPIYIIRAFWMPFLLFHLGGSDTITAYSIQDNDLWLRALFRFITNLL 126
            +  G     +       + A W PFLL HLGG DTITA++++DN LWLR +F  +   +
Sbjct: 73  SKNQGKDLKPDDPPQDKKVMALWAPFLLLHLGGPDTITAFALEDNALWLRHVFGLVFQAI 132

Query: 127 LSFYIFMLALNRPKALNYIAIPVFVAGIIKYAQKICALRSASGQRLRDLLVSPPPPFRPN 186
              Y+ +++L  P +L  + + VFV+G IKY ++  AL SAS  + RD ++  P P  PN
Sbjct: 133 AGVYVVVMSL--PNSLWVVIVLVFVSGTIKYLERTTALYSASLDKFRDSMIQAPDP-GPN 192

Query: 187 ----------TQNFAFPIKIT-STEMDQLN----------VSNNNDPYFSDLHMLHLAYC 246
                      +    P KI    E D+ N           S       +DL ++  AY 
Sbjct: 193 YAKLMEEYKAKKEARLPTKIVLIDEPDKENRPKKLEHPALASKKRKKDLTDLEIVQYAYK 252

Query: 247 FFKRDKRLFVDLDLSSYHLQQSLYYFEKF-DSKTAFKVIELELGFIYDFFYTKAFVIHSR 306
           FF   K L V+L  S     +SL  FE   D + A ++IE+ELGF+YD  +TK  ++H+ 
Sbjct: 253 FFNTFKGLVVNLIFSFRERDESLEIFENLNDPEEALRIIEIELGFLYDALFTKIAILHTG 312

Query: 307 WGRVLRLTSVSSLVIAMVAF-SCMSDWQDYPLKYVRLTFILFSAALGLEIYSLLLFLHSD 366
            G V R+ +  +LV A + F    +   D+    V +T+ LF+  L L+  S+LLFL SD
Sbjct: 313 IGTVSRVFASGTLVAAFIIFHKKPNKGTDFHGADVVVTYTLFAVGLVLDFISILLFLFSD 372

Query: 367 W-----------------------NVISLTRSSYSLPRLIFKLISH-------------- 426
           W                       N +   R     P+   K   H              
Sbjct: 373 WTCAAYSSLKDDPDELQSWQERCFNWLLKFRKLRWTPQECHKTGMHKCTKEGLKPCIMKG 432

Query: 427 ---------------------------CG---------WSVWKRRWSNSTSQYNIISRCL 486
                                      C             + RRWS S + +N I+   
Sbjct: 433 ADKKEGGNKNEGGNKKEGDDKKEAADKCSLVDKHDVLTTRFFLRRWSGSINVFNFIAYAT 492

Query: 487 KEK----NDGRYYYRK-------------------------------------------- 546
           K      +D R  +R+                                            
Sbjct: 493 KADVERIHDARGSFRRYSWKIITFPFKKLNFVIKKIFGSIVKLINEVHRRISHEVNALSR 552

Query: 547 ----------------------FLRSI--------------SCITKITAANSRCISNDLQ 606
                                 F++S+                + K    +   ++ +L 
Sbjct: 553 KHLWARRFLYPIYFEFISRIPHFIKSVWDILSEFFDISDTLDMVHKTLFVHGEPMTRELW 612

Query: 607 TRIFEQLKEKKL--EANEANKKLP----GWILRKH-----NCYNQLGWSLKLDFDESILL 643
             IFE+LK K    ++ E  K++      W LR++          + +  K+D+D+S+L+
Sbjct: 613 KFIFEELKNKSKYGDSPENAKRISLARGEWTLRENLPVDAEREKLVRYVTKVDYDQSLLM 672

BLAST of Spg022739 vs. TAIR 10
Match: AT5G45480.1 (Protein of unknown function (DUF594) )

HSP 1 Score: 213.4 bits (542), Expect = 5.8e-55
Identity = 215/853 (25.21%), Postives = 340/853 (39.86%), Query Frame = 0

Query: 7   WDFMYEWELVFLLVVSLSLQLVLVPMGSKRKYSSNNCLRLFLLIAYSSADWIVMVSLGIV 66
           WD   EW +   L+ SLSLQ  L+    +RK SS   L  F+  AY  ADW    + G +
Sbjct: 13  WD---EWSIRSTLIFSLSLQTFLIFFAPQRKRSSRKVLLSFIWSAYLLADWSANFAAGQI 72

Query: 67  IEKYGGGCYDEFSDPIYIIRAFWMPFLLFHLGGSDTITAYSIQDNDLWLRALFRFITNLL 126
            +  G             + AFW+PFLL HLGG DTITA +++DN+LWLR L       +
Sbjct: 73  SDSQGDDPEPGEPKKSAELFAFWVPFLLLHLGGPDTITALALEDNELWLRHLLGLFFQSV 132

Query: 127 LSFYIFMLALNRPKALNYIAIPVFVAGIIKYAQKICALRSASGQRLRDLLVSPPPPFRPN 186
            + Y+ + +L  P AL    + VF  G+IKY ++  AL  AS  + +D ++  P P  PN
Sbjct: 133 ATVYVLLQSL--PNALWKPILLVFATGVIKYVERTLALYLASLDKFKDSMIQRPDP-GPN 192

Query: 187 ----------TQNFAFP---IKITSTEMDQLNVSNNNDP-YFSDLHMLHLAYCFFKRDKR 246
                      ++   P   IK+   E D  + +    P  F+ L++L  AY +F   K 
Sbjct: 193 YAKLMEEYAAKKDMKMPTQIIKVGEPEKDPRDDAPVKPPDGFTPLNILQYAYKYFNIFKG 252

Query: 247 LFVDLDLSSYHLQQSLYYFEKFDSKTAFKVIELELGFIYDFFYTKAFVIHSRWGRVLRLT 306
           L VDL  +     +S  +F+   ++ A +++E+EL FIY   YTKA ++H+  G + R  
Sbjct: 253 LVVDLIFTFQQRAESKRFFDSLKAEEALRILEVELNFIYAALYTKAEILHNWIGFLFRFI 312

Query: 307 SVSSLVIAMVAFSCMSDWQDYPLKYVRLTFILFSAALGLEIYSLLLFLHSDWNVISL--- 366
           ++  L  A+  F   S  +DY    V LT+ L    + L+  +L++F  SDW  + L   
Sbjct: 313 ALGCLAAALRIFQYKSK-KDYSGFDVGLTYALLLGGIALDCIALIMFCASDWTFVRLRKM 372

Query: 367 -------------------------------------------------TRSSYSLPRLI 426
                                                            T+ +  L R++
Sbjct: 373 KDEVDDPPTWSDNILNWILENILGVRKLKVEEYDECYKNTQSHEVPNTSTKKTPFLKRIL 432

Query: 427 FKLI---------SH----------------------------CGWSVWKRRWSNSTSQY 486
            +++         SH                               S   RRWS     +
Sbjct: 433 NRILRVRELKTEKSHEVLDKSTSKIPGQEVPDKSAKTIPCHKVLDTSFMYRRWSEYVHAH 492

Query: 487 NIISRCLKEKNDGRYYYRKFLR-------------------------------------- 546
           N+I  CL  K    ++ + F+                                       
Sbjct: 493 NLIEYCLGLKPKRIHHTKGFIHIAFDKVINILYIGPAFTKLGSVIESCFRVTKQRIHQTF 552

Query: 547 ------------------------SISCITKITAANSRCISN------------------ 606
                                   S  CI  I +   R I +                  
Sbjct: 553 KWIDGKVSRLCKKYPKLNEEYIRFSFFCIFYIPSLPGRWIKSFMEFFGIRAQLDEVIYTS 612

Query: 607 ------DLQTRIFEQLKEKKLEANEANKKL------PGWILR--------KHNCYNQLGW 639
                 D+   IF ++K K   A+++   +        W LR        +      L +
Sbjct: 613 SDRLTLDMWEHIFGEVKAKSRFADDSESAMRVSSARGDWTLRDIQGDPETEKKREKLLRY 672

BLAST of Spg022739 vs. TAIR 10
Match: AT5G45530.1 (Protein of unknown function (DUF594) )

HSP 1 Score: 209.5 bits (532), Expect = 8.3e-54
Identity = 203/771 (26.33%), Postives = 320/771 (41.50%), Query Frame = 0

Query: 12  EWELVFLLVVSLSLQLVLVPMGSKRKYSSNNCLRLFLLIAYSSADWIVMVSLGIVIEKYG 71
           +W +  L+++SL  Q  L+ +   RK +S   L   L  AY  ADW    ++  + +  G
Sbjct: 14  KWNIRGLVIMSLLFQTSLIFLAPMRKRTSKKLLAAVLWTAYLLADWTANYAVSQITKNQG 73

Query: 72  GGCYDEFSDPIYIIRAFWMPFLLFHLGGSDTITAYSIQDNDLWLRALFRFITNLLLSFYI 131
                        + A W PFLL HLGG DTITA +++DN LW R LF  ++  L   Y 
Sbjct: 74  KETEPGDPPKNKKLLALWAPFLLLHLGGPDTITALALEDNALWQRHLFGLVSQALAGVYA 133

Query: 132 FMLALNRPKALNYIAIPV---FVAGIIKYAQKICALRSASGQRLRDLLVSPPPP---FRP 191
            + +L      N +  P+   F+ G IKY ++  AL SAS  + +D ++        +  
Sbjct: 134 VVQSLE-----NVLWPPITLLFITGTIKYVERTRALYSASLDKFKDRMLQRADAGSNYAK 193

Query: 192 NTQNFA------FPIKITSTE----MDQLNVSNNNDPYFSDLHMLHLAYCFFKRDKRLFV 251
             + FA       P +I  T+     ++       D   +DL ++   + FF   K L V
Sbjct: 194 LMEEFASRKMSNLPTEIFLTDEPDKHERPPTLVKPDRDLTDLEIVQYGFKFFNTFKGLVV 253

Query: 252 DLDLSSYHLQQSLYYFEKFDSKTAFKVIELELGFIYDFFYTKAFVIHSRWGRVLRLTSVS 311
           DL  S     +S  +F++     A ++IE ELGF+Y+  YTK  ++H+  G + RL S  
Sbjct: 254 DLIFSFRERDESRDFFKELKPGEALRIIETELGFLYESMYTKTAILHTGIGTLFRLISFG 313

Query: 312 SLVIAMVAFSCMS-DWQDYPLKYVRLTFILFSAALGLEIYSLLLFLHSDWNVISLTRSSY 371
           SL+ +   F       +D+    V +T++LF   + L++ S+++FL SDW    L     
Sbjct: 314 SLLSSFFVFHRRPLKSEDFHGADVVITYVLFIVGIALDLASMVIFLLSDWTFAVLRNLKD 373

Query: 372 SLPRLIFKLISHCGWSV------WK-------------------RRWSNSTSQYNIISRC 431
                   + S   W +      WK                   RRWS +   +N I  C
Sbjct: 374 DPEEKSTSIDSLFNWFLEFRKPRWKKHTCNGNQTHEVLSTGFFTRRWSGTIYGFNFIGFC 433

Query: 432 LKEKNDGRYYYR--------------------------------KFLRSI---------- 491
           LK K    +  R                                + +RS+          
Sbjct: 434 LKAKVSRIHQKRNCNLLVWDYVVSLFDLVIRRIQMMIGWIKNVNRSIRSVLRQWSKKNPM 493

Query: 492 ------------------------SCITKITAANS-----RCISNDLQTR-----IFEQL 551
                                     I +I +  S     R IS +  T+     IF ++
Sbjct: 494 IRCTVYPLYLVFFAGIPEVFRVLWKYIDRIFSVTSYLDGIRFISREPLTKNQWEFIFNEV 553

Query: 552 KEKK--LEANEANKKLP----GWILRKHNCY---NQLGWSLKLDFDESILLWHIATDICY 611
           K+K    E  E  KK+      W LR          + +  K+D+D+S+LLWHIAT++C+
Sbjct: 554 KDKSGFAETPEVAKKVSWARGEWALRDSKLMEVDTLMRYIEKVDYDQSLLLWHIATELCF 613

Query: 612 HNNSDAASIDSSL-----LEDSVLLSDFLTDILAQRPWLFPN--GMSQIRLSATNDDVVE 639
                      S       E S ++SD++  +L  RP L     G+  IR   T  +   
Sbjct: 614 QKEEGGKMEKLSREGYDDREFSKIISDYMMYLLIMRPKLMSEVAGIGTIRFRDTKAEAER 673

BLAST of Spg022739 vs. TAIR 10
Match: AT5G45460.1 (unknown protein; BEST Arabidopsis thaliana protein match is: Protein of unknown function (DUF594) (TAIR:AT5G45470.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 161.0 bits (406), Expect = 3.4e-39
Identity = 119/367 (32.43%), Postives = 184/367 (50.14%), Query Frame = 0

Query: 7   WDFMYEWELVFLLVVSLSLQLVLVPMGSKRKYSSNNCLRLFLLIAYSSADWIVMVSLGIV 66
           WD    W +   + +SL+LQ  L+     RK +    L + +  +Y  ADW    ++G++
Sbjct: 13  WD---RWNIRGTIFLSLTLQAFLICFSPLRKRTPRRHLIIVIWSSYLLADWSANFAVGLI 72

Query: 67  IEKYGGGCYDEFSDPIYIIRAFWMPFLLFHLGGSDTITAYSIQDNDLWLRALFRFITNLL 126
            +  G     +       + A W PFLL HLGG DTITA++++DN LWLR +F  +   +
Sbjct: 73  SKNQGKDLKPDDPPQDKKLMALWAPFLLLHLGGPDTITAFALEDNALWLRNVFGLVFQAI 132

Query: 127 LSFYIFMLALNRPKALNYIAIPVFVAGIIKYAQKICALRSASGQRLRDLLVSPPPPFRPN 186
              Y+ + +L  P +L    + VF++G IKY ++  AL SAS  + RD ++  P P  PN
Sbjct: 133 AGVYVVLQSL--PNSLWVTILLVFISGTIKYLERTTALYSASLDKFRDSMIQGPDP-GPN 192

Query: 187 ----------TQNFAFPIK-ITSTEMDQLN----------VSNNNDPYFSDLHMLHLAYC 246
                      +    P K I   E D+ +           S       + L +   AY 
Sbjct: 193 YAKLMEEYKAKKEAKLPTKIILIDEPDKEHRPKKLEHPSLASETKRKELTHLEIAQYAYK 252

Query: 247 FFKRDKRLFVDLDLSSYHLQQSLYYFEKF-DSKTAFKVIELELGFIYDFFYTKAFVIHSR 306
           FF   K L V+L  S     QS+  F+   D + A ++IE+ELGF+YD  +TK  V+H+ 
Sbjct: 253 FFNTFKGLVVNLIFSFRERDQSIEIFQNLEDPEEALRIIEIELGFLYDALFTKNAVLHTV 312

Query: 307 WGRVLRLTSVSSLVIAMVAFSCMSD-WQDYPLKYVRLTFILFSAALGLEIYSLLLFLHSD 351
            G V R+ +  SLV A + F  +S+  +D+    V +T+ILF+  L L+  S+LLFL SD
Sbjct: 313 LGTVSRVVASGSLVAAFIIFHKISNKGRDFHGADVVITYILFAVGLVLDFISILLFLFSD 372

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022141971.14.3e-19357.73uncharacterized protein LOC111012216 [Momordica charantia][more]
XP_004139148.12.9e-17352.70uncharacterized protein LOC101222078 [Cucumis sativus] >KGN66604.1 hypothetical ... [more]
KAA0037446.13.7e-17353.21uncharacterized protein E6C27_scaffold277G00320 [Cucumis melo var. makuwa] >TYK0... [more]
XP_008458716.16.0e-17152.62PREDICTED: uncharacterized protein LOC103498043 [Cucumis melo][more]
GFZ17712.11.1e-12941.60transmembrane protein, putative [Actinidia rufa][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1CKT22.1e-19357.73uncharacterized protein LOC111012216 OS=Momordica charantia OX=3673 GN=LOC111012... [more]
A0A0A0LZZ21.4e-17352.70DUF4220 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G638510 PE=... [more]
A0A5D3BS411.8e-17353.21DUF4220 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3C8L72.9e-17152.62uncharacterized protein LOC103498043 OS=Cucumis melo OX=3656 GN=LOC103498043 PE=... [more]
A0A5B7BVN36.5e-13141.45DUF4220 domain-containing protein OS=Davidia involucrata OX=16924 GN=Din_042423 ... [more]
Match NameE-valueIdentityDescription
AT5G45540.12.0e-6026.09Protein of unknown function (DUF594) [more]
AT5G45470.11.2e-5726.07Protein of unknown function (DUF594) [more]
AT5G45480.15.8e-5525.21Protein of unknown function (DUF594) [more]
AT5G45530.18.3e-5426.33Protein of unknown function (DUF594) [more]
AT5G45460.13.4e-3932.43unknown protein; BEST Arabidopsis thaliana protein match is: Protein of unknown ... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025315Domain of unknown function DUF4220PFAMPF13968DUF4220coord: 50..387
e-value: 6.1E-66
score: 223.2
IPR007658Protein of unknown function DUF594PFAMPF04578DUF594coord: 589..638
e-value: 6.3E-20
score: 70.5
NoneNo IPR availablePANTHERPTHR31325:SF207OS08G0149333 PROTEINcoord: 6..639
NoneNo IPR availablePANTHERPTHR31325OS01G0798800 PROTEIN-RELATEDcoord: 6..639

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg022739.1Spg022739.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane