Spg022731 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg022731
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	exopolygalacturonase-like
Location	scaffold2: 13444271 .. 13446317 (+)
RNA-Seq Expression	Spg022731
Synteny	Spg022731

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACAACGTCAAGAAGTTTTAGCCTCTTCCAAATCCTTCTCTTGGTATTTGCTTGGCAATGTTGTGCAATGGTAGTTGCCATTTTCGACAACGAGGTCGGTATTGTGAATCTCGTCGGCGGGATTGCATCAACGGTAAATCAACAACTTCCTACATCTGCTCCTCAGACTCTTGGTATCGGAATTCTGACAGGTAGCAGCGATAGTGTTGTAGTAAATGATGTTAAGGCCGCTGTTGATCTGAATGGGAAGGGTGGTGCAGTTTTTGATGTTAGAAAGTATGGAGCTAAAGCTGATGGAAAGACTGATAATGCACAGGTGATGATATAATTAACGTCAATGAATCATAGACTTTAAACTTTTAATTTGAATAATTATCTTTGAACTTATAAAATTATTGAAATTTTAGCAACGATCATCTAAATATATGCTACTCCATAAATTTGCCAACTTAAACAAGTCATCTGGTAAAAACAATCTCTTTCTAATTTAACTGGCATCTTTTTTCTACTTTTCTAAAAAAAAATAACTTTGATTTTCCACGTGGTAGGCATTCATGACGACATGGATTGCAGCATGTCAGAAGACAGTCGGTCCGGCGAAGTTTTTGATCCCACGGGGCACATTTCTTGTGGGTCCGGTGACTTTTGCTGGTCCGTGCAAAAGCTTGCCGATCACCATAGAAATTCAAGGAACTGTAAAGGCCACAACCGATATCAGTGAATATTCTTCCCCTGAATGGTTCTCTATCGAACATATCACCGGCCTTATCCTCACCGGCTCCGACGTCTTCGACGGCCAAGGTGCCTCCGCCTGGTCCTACAATGACTGCAAGAAAAACATTCACTGCCAGAGTCTTCCAATCGTAACTATAATTTTCCATAACTCAATCACCTCCTATTTTTATTATAAATTACAGCTATAAATTTTTAAAGTTATGTCTAATTAATACGAAGTCGGGAATTCACTATTAAAATTCAGTAATTCACTATTAAAAAAATGTGTCATGACTAAATTTATAATTTGTTCTAATTGTTATTGTGATTAATTAATTGGTTTTTTTTTCAGTCGATTAAATTCTGGAAATTGAACCACACAATTGTTGATGGGATCACTTCCTTGAACAGCAAGGCCTTTCACACGTCTGTTTTCAACTGCTATAATTTCACTGCAACCAACATGCGCATTATAGCCCCCGGAAATAGTCCCAACACCGACGGAATGCACCTTAGCACCTCGAAATTGGTCACCATTTCTAACAGCGTCATTGGCACCGGTGACGACTGTGTCTCTATTGGCCAAGGCTGTCAGAAAATCACTATCACTAATATCACTTGCGGTCCTGGACATGGTATTAGGTAATTATATCTTTTTGTAAAAAAAAATAAAGATTAAATAGACACAAATCTTCGAAGTTTCTAATTTTCATAGACGAAGAAAAGCAACTCAAAAGTTGTTTATCCTCTTTACAGTGTGGGCAGCTTGGGTAAGTATCCTAAAGAAAAGAGTGTCTTCGGCGTTCTTGTGCAAAACTGCACCATTTTTAACGCCACCAACGGTGCTAGAATCAAGACATGGGCTGACACAGTGTCGGGGTCGGCCACAGGAATAATCTTTGATGACATCGTAATGTACAACGTCAAATACCCCATAATCATCGATCAAACTTATGGCACAAAGAAGAACAAGGTAACAACCACAGAAAAACAAACTAATCTACGTTCTTTTCAAAACATTTATCATAAACCATCAAGTTAATTAAGCTTTTGAGTTTAATAGTAATTTTACGCTCTACAATTTGATCATGTAGGCTTCAAAGTGGAAGATCAGCGACGTTCACTTCAAGAACATCCGCGGGACGTCGGGGACGAACGTGGCAGTGTTGTTGGAGTGCAGCGCGTTGTTTCCGTGCAAGGGGGTGGAGCTGAGGGACATTAACTTGACCTATGGGGGCAACAACTTGAGGAATACGACCATTGTTTCTTCATGTTTGAATGCAAAAATTGAACATCATGGGGTGCAGAACCCGCCGCCTTGTGTTGTATAA

mRNA sequence

ATGACAACGTCAAGAAGTTTTAGCCTCTTCCAAATCCTTCTCTTGGTATTTGCTTGGCAATGTTGTGCAATGGTAGTTGCCATTTTCGACAACGAGGTCGGTATTGTGAATCTCGTCGGCGGGATTGCATCAACGGTAAATCAACAACTTCCTACATCTGCTCCTCAGACTCTTGGTATCGGAATTCTGACAGGTAGCAGCGATAGTGTTGTAGTAAATGATGTTAAGGCCGCTGTTGATCTGAATGGGAAGGGTGGTGCAGTTTTTGATGTTAGAAAGTATGGAGCTAAAGCTGATGGAAAGACTGATAATGCACAGAAGACAGTCGGTCCGGCGAAGTTTTTGATCCCACGGGGCACATTTCTTGTGGGTCCGGTGACTTTTGCTGGTCCGTGCAAAAGCTTGCCGATCACCATAGAAATTCAAGGAACTGTAAAGGCCACAACCGATATCAGTGAATATTCTTCCCCTGAATGGTTCTCTATCGAACATATCACCGGCCTTATCCTCACCGGCTCCGACGTCTTCGACGGCCAAGGTGCCTCCGCCTGGTCCTACAATGACTGCAAGAAAAACATTCACTGCCAGAGTCTTCCAATCTCGATTAAATTCTGGAAATTGAACCACACAATTGTTGATGGGATCACTTCCTTGAACAGCAAGGCCTTTCACACGTCTGTTTTCAACTGCTATAATTTCACTGCAACCAACATGCGCATTATAGCCCCCGGAAATAGTCCCAACACCGACGGAATGCACCTTAGCACCTCGAAATTGGTCACCATTTCTAACAGCGTCATTGGCACCGGTGACGACTGTGTCTCTATTGGCCAAGGCTGTCAGAAAATCACTATCACTAATATCACTTGCGGTCCTGGACATGGTATTAGTGTGGGCAGCTTGGGTAAGTATCCTAAAGAAAAGAGTGTCTTCGGCGTTCTTGTGCAAAACTGCACCATTTTTAACGCCACCAACGGTGCTAGAATCAAGACATGGGCTGACACAGTGTCGGGGTCGGCCACAGGAATAATCTTTGATGACATCGTAATGTACAACGTCAAATACCCCATAATCATCGATCAAACTTATGGCACAAAGAAGAACAAGGCTTCAAAGTGGAAGATCAGCGACGTTCACTTCAAGAACATCCGCGGGACGTCGGGGACGAACGTGGCAGTGTTGTTGGAGTGCAGCGCGTTGTTTCCGTGCAAGGGGGTGGAGCTGAGGGACATTAACTTGACCTATGGGGGCAACAACTTGAGGAATACGACCATTGTTTCTTCATGTTTGAATGCAAAAATTGAACATCATGGGGTGCAGAACCCGCCGCCTTGTGTTGTATAA

Coding sequence (CDS)

ATGACAACGTCAAGAAGTTTTAGCCTCTTCCAAATCCTTCTCTTGGTATTTGCTTGGCAATGTTGTGCAATGGTAGTTGCCATTTTCGACAACGAGGTCGGTATTGTGAATCTCGTCGGCGGGATTGCATCAACGGTAAATCAACAACTTCCTACATCTGCTCCTCAGACTCTTGGTATCGGAATTCTGACAGGTAGCAGCGATAGTGTTGTAGTAAATGATGTTAAGGCCGCTGTTGATCTGAATGGGAAGGGTGGTGCAGTTTTTGATGTTAGAAAGTATGGAGCTAAAGCTGATGGAAAGACTGATAATGCACAGAAGACAGTCGGTCCGGCGAAGTTTTTGATCCCACGGGGCACATTTCTTGTGGGTCCGGTGACTTTTGCTGGTCCGTGCAAAAGCTTGCCGATCACCATAGAAATTCAAGGAACTGTAAAGGCCACAACCGATATCAGTGAATATTCTTCCCCTGAATGGTTCTCTATCGAACATATCACCGGCCTTATCCTCACCGGCTCCGACGTCTTCGACGGCCAAGGTGCCTCCGCCTGGTCCTACAATGACTGCAAGAAAAACATTCACTGCCAGAGTCTTCCAATCTCGATTAAATTCTGGAAATTGAACCACACAATTGTTGATGGGATCACTTCCTTGAACAGCAAGGCCTTTCACACGTCTGTTTTCAACTGCTATAATTTCACTGCAACCAACATGCGCATTATAGCCCCCGGAAATAGTCCCAACACCGACGGAATGCACCTTAGCACCTCGAAATTGGTCACCATTTCTAACAGCGTCATTGGCACCGGTGACGACTGTGTCTCTATTGGCCAAGGCTGTCAGAAAATCACTATCACTAATATCACTTGCGGTCCTGGACATGGTATTAGTGTGGGCAGCTTGGGTAAGTATCCTAAAGAAAAGAGTGTCTTCGGCGTTCTTGTGCAAAACTGCACCATTTTTAACGCCACCAACGGTGCTAGAATCAAGACATGGGCTGACACAGTGTCGGGGTCGGCCACAGGAATAATCTTTGATGACATCGTAATGTACAACGTCAAATACCCCATAATCATCGATCAAACTTATGGCACAAAGAAGAACAAGGCTTCAAAGTGGAAGATCAGCGACGTTCACTTCAAGAACATCCGCGGGACGTCGGGGACGAACGTGGCAGTGTTGTTGGAGTGCAGCGCGTTGTTTCCGTGCAAGGGGGTGGAGCTGAGGGACATTAACTTGACCTATGGGGGCAACAACTTGAGGAATACGACCATTGTTTCTTCATGTTTGAATGCAAAAATTGAACATCATGGGGTGCAGAACCCGCCGCCTTGTGTTGTATAA

Protein sequence

MTTSRSFSLFQILLLVFAWQCCAMVVAIFDNEVGIVNLVGGIASTVNQQLPTSAPQTLGIGILTGSSDSVVVNDVKAAVDLNGKGGAVFDVRKYGAKADGKTDNAQKTVGPAKFLIPRGTFLVGPVTFAGPCKSLPITIEIQGTVKATTDISEYSSPEWFSIEHITGLILTGSDVFDGQGASAWSYNDCKKNIHCQSLPISIKFWKLNHTIVDGITSLNSKAFHTSVFNCYNFTATNMRIIAPGNSPNTDGMHLSTSKLVTISNSVIGTGDDCVSIGQGCQKITITNITCGPGHGISVGSLGKYPKEKSVFGVLVQNCTIFNATNGARIKTWADTVSGSATGIIFDDIVMYNVKYPIIIDQTYGTKKNKASKWKISDVHFKNIRGTSGTNVAVLLECSALFPCKGVELRDINLTYGGNNLRNTTIVSSCLNAKIEHHGVQNPPPCVV

Homology

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BLAST of Spg022731 vs. NCBI nr
Match: XP_038896336.1 (exopolygalacturonase-like [Benincasa hispida])

HSP 1 Score: 689.5 bits (1778), Expect = 1.9e-194
Identity = 344/458 (75.11%), Postives = 378/458 (82.53%), Query Frame = 0


Query: 3   TSRSFSLFQILLLVFAWQCCAMVVAIFDNEVGIVNLVGGIASTVNQQL--PTSAPQTLGI 62
           T  S SLFQILLL FAWQCCA V AIFD+  G  NLV G+ STVNQ L  P +AP  LGI
Sbjct: 2   TMTSPSLFQILLLAFAWQCCAEVAAIFDDITGTANLVNGVVSTVNQPLLHPAAAPLPLGI 61

Query: 63  GILTGSSDSVVVNDVKAAVDLNGKGGAVFDVRKYGAKADGKTDNAQ-----------KTV 122
           G L     +  VND+KA VDLNG GG+VFDV K+GAK DGKTD+AQ              
Sbjct: 62  GTLPDLGGT--VNDIKANVDLNGNGGSVFDVTKHGAKGDGKTDDAQAFMTTWIEACRNAE 121

Query: 123 GPAKFLIPRGTFLVGPVTFAGPCKSLPITIEIQGTVKATTDISEYSSPEWFSIEHITGLI 182
           GPAKFLIP+GTFLVGPVTFAGPCKS PIT+E QGTVKATTDI++YSSPEWFSIE ITG I
Sbjct: 122 GPAKFLIPQGTFLVGPVTFAGPCKSFPITLENQGTVKATTDITQYSSPEWFSIEEITGFI 181

Query: 183 LTGSDVFDGQGASAWSYNDCKKNIHCQSLPISIKFWKLNHTIVDGITSLNSKAFHTSVFN 242
           LTGS VFDGQGA++W YNDCKKN  CQ LPISIKF KLNHTIVDG+ SLNSK FHTS+F 
Sbjct: 182 LTGSGVFDGQGAASWPYNDCKKNTKCQILPISIKFTKLNHTIVDGLASLNSKGFHTSIFY 241

Query: 243 CYNFTATNMRIIAPGNSPNTDGMHLSTSKLVTISNSVIGTGDDCVSIGQGCQKITITNIT 302
           CYNFTATNM IIAPGNSPNTDGMH+STSKLVTI+NSVIGTGDDCVSIG   +KI +TN+T
Sbjct: 242 CYNFTATNMNIIAPGNSPNTDGMHISTSKLVTITNSVIGTGDDCVSIGHSTEKIVVTNVT 301

Query: 303 CGPGHGISVGSLGKYPKEKSVFGVLVQNCTIFNATNGARIKTWADTVSGSATGIIFDDIV 362
           CGPGHG+SVGSLGKY KEKSV+ VLV+NCTIFNATNGARIKTWA  +SG A+GI F+DI+
Sbjct: 302 CGPGHGLSVGSLGKYSKEKSVYDVLVKNCTIFNATNGARIKTWASPISGLASGITFEDII 361

Query: 363 MYNVKYPIIIDQTYGTKKNKASKWKISDVHFKNIRGTSGTNVAVLLECSALFPCKGVELR 422
           MYNVK PIIIDQTYGTK+ KAS WKISDVHFKNIRGTS TNVAV LECS L PC+ VELR
Sbjct: 362 MYNVKNPIIIDQTYGTKQKKASNWKISDVHFKNIRGTSTTNVAVSLECSELLPCEEVELR 421

Query: 423 DINLTYGGNNLRNTTIVSSCLNAKIEHHGVQNPPPCVV 448
           DINLTYGG NLRNTTI+SSC NAKI+ +G+QNPP CVV
Sbjct: 422 DINLTYGGPNLRNTTILSSCSNAKIDSYGMQNPPACVV 457

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BLAST of Spg022731 vs. NCBI nr
Match: XP_022991773.1 (exopolygalacturonase-like [Cucurbita maxima])

HSP 1 Score: 688.7 bits (1776), Expect = 3.3e-194
Identity = 345/459 (75.16%), Postives = 381/459 (83.01%), Query Frame = 0


Query: 1   MTTSRSFSLFQILLLVFAWQCCAMVVAIFDNEVGIVNLVGGIASTVNQQ-LPTSAPQTLG 60
           MTT+R+ SLFQ LLLV A QCCA V A+FD+  G  NL+ GI ST NQQ  P +AP  LG
Sbjct: 1   MTTTRNLSLFQTLLLVLACQCCAEVAAVFDHVTGTANLIDGIVSTANQQHRPPAAPLPLG 60

Query: 61  IGILTGSSDSVVVNDVKAAVDLNGKGGAVFDVRKYGAKADGKTDNAQ-----------KT 120
           I        SV +N  KAAV L G G AVFDV+KYGAKA+GK+D+AQ            T
Sbjct: 61  IRTPANRRGSVAINGTKAAVYLKGNGNAVFDVKKYGAKANGKSDDAQAFMTTWIAACRNT 120

Query: 121 VGPAKFLIPRGTFLVGPVTFAGPCKSLPITIEIQGTVKATTDISEYSSPEWFSIEHITGL 180
            GPAKFLIP+G FLVGPVTFAGPCKS+PITIEIQGTVKATTDISEYSSP+W SIE ITGL
Sbjct: 121 TGPAKFLIPKGIFLVGPVTFAGPCKSMPITIEIQGTVKATTDISEYSSPDWISIEGITGL 180

Query: 181 ILTGSDVFDGQGASAWSYNDCKKNIHCQSLPISIKFWKLNHTIVDGITSLNSKAFHTSVF 240
           ILTGS VFDGQGASAW YNDCK N +CQ LP SIKF +LNH+IVDG+TS+NSK FHTSVF
Sbjct: 181 ILTGSGVFDGQGASAWPYNDCKINNNCQILPNSIKFTRLNHSIVDGLTSINSKGFHTSVF 240

Query: 241 NCYNFTATNMRIIAPGNSPNTDGMHLSTSKLVTISNSVIGTGDDCVSIGQGCQKITITNI 300
            CYNFTATNM I+APGNSPNTDGMHLSTSKLVTIS+S IGTGDDCVSIG  C+KIT+TN+
Sbjct: 241 RCYNFTATNMNIMAPGNSPNTDGMHLSTSKLVTISSSTIGTGDDCVSIGHSCEKITVTNV 300

Query: 301 TCGPGHGISVGSLGKYPKEKSVFGVLVQNCTIFNATNGARIKTWADTVSGSATGIIFDDI 360
           TCGPGHGIS+GSLG+Y  EKSV  VLVQNCTIFNATNGARIKTWA+T+SGSA GI+FD+I
Sbjct: 301 TCGPGHGISIGSLGRYKTEKSVLDVLVQNCTIFNATNGARIKTWANTISGSAVGIVFDNI 360

Query: 361 VMYNVKYPIIIDQTYGTKKNKASKWKISDVHFKNIRGTSGTNVAVLLECSALFPCKGVEL 420
           VM  VK PIIIDQTYGTK+ KASKWKISDVHFKNIRGTS TNVAVLLECS LFPC+GVEL
Sbjct: 361 VMRKVKNPIIIDQTYGTKEKKASKWKISDVHFKNIRGTSTTNVAVLLECSELFPCEGVEL 420

Query: 421 RDINLTYGGNNLRNTTIVSSCLNAKIEHHGVQNPPPCVV 448
           RDINL+YGG +L+NTT VSSCLNAKI+  GVQNPP CVV
Sbjct: 421 RDINLSYGGASLKNTTTVSSCLNAKIKTFGVQNPPACVV 459

[Close]

BLAST of Spg022731 vs. NCBI nr
Match: KAG7014351.1 (Exopolygalacturonase-like 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 688.3 bits (1775), Expect = 4.3e-194
Identity = 349/461 (75.70%), Postives = 380/461 (82.43%), Query Frame = 0


Query: 1   MTTSRSFSLFQILLLVFAWQCCAMVVAIFDNEVGIVNLVGGIASTVNQQ-LPTSAPQTLG 60
           MTT+ + SLFQ LLLV A QCCA V A+FD+     N + GI ST NQQ  P + P  LG
Sbjct: 1   MTTTTNLSLFQTLLLVLACQCCAEVAAVFDHVKVAANPIDGIVSTANQQHRPPAPPLPLG 60

Query: 61  IGILTGSSDSVVVNDVKAAVDL--NGKGGAVFDVRKYGAKADGKTDNAQ----------- 120
           IG L  S   V +N  KAAVDL  NG G AVFDV+KYGAKA+GK+D+AQ           
Sbjct: 61  IGTLANSRGRVAINSTKAAVDLKGNGNGNAVFDVKKYGAKANGKSDDAQAFMTTWIAACR 120

Query: 121 KTVGPAKFLIPRGTFLVGPVTFAGPCKSLPITIEIQGTVKATTDISEYSSPEWFSIEHIT 180
            T GPAKFLIP+GTFLVGPV FAGPC+S PITIEIQGTVKATTDISEYSSP+W SIE IT
Sbjct: 121 NTTGPAKFLIPKGTFLVGPVVFAGPCQSRPITIEIQGTVKATTDISEYSSPDWISIEGIT 180

Query: 181 GLILTGSDVFDGQGASAWSYNDCKKNIHCQSLPISIKFWKLNHTIVDGITSLNSKAFHTS 240
           GLILTGS VFDGQGASAW YNDCK N +CQ LP SIKF +LNH+IVDG+TS+NSK FHTS
Sbjct: 181 GLILTGSGVFDGQGASAWPYNDCKTNNNCQILPNSIKFTRLNHSIVDGLTSINSKGFHTS 240

Query: 241 VFNCYNFTATNMRIIAPGNSPNTDGMHLSTSKLVTISNSVIGTGDDCVSIGQGCQKITIT 300
           VF CYNFTATNM IIAPGNSPNTDGMHLSTSKLVTIS+S IGTGDDCVSIG  C+KIT+T
Sbjct: 241 VFRCYNFTATNMNIIAPGNSPNTDGMHLSTSKLVTISSSTIGTGDDCVSIGHSCEKITVT 300

Query: 301 NITCGPGHGISVGSLGKYPKEKSVFGVLVQNCTIFNATNGARIKTWADTVSGSATGIIFD 360
           N+TCGPGHGIS+GSLGKY  EKSV  VLVQNCTIFNATNGARIKTWADT+SGSA GIIFD
Sbjct: 301 NVTCGPGHGISIGSLGKYKTEKSVLDVLVQNCTIFNATNGARIKTWADTISGSAVGIIFD 360

Query: 361 DIVMYNVKYPIIIDQTYGTKKNKASKWKISDVHFKNIRGTSGTNVAVLLECSALFPCKGV 420
           +IVM  VK PIIIDQTYGTKK KASKWKIS+VHFKNIRGTS TNVAVLLECS LFPC+GV
Sbjct: 361 NIVMRKVKNPIIIDQTYGTKKKKASKWKISNVHFKNIRGTSTTNVAVLLECSELFPCEGV 420

Query: 421 ELRDINLTYGGNNLRNTTIVSSCLNAKIEHHGVQNPPPCVV 448
           ELRDINL+YGG +L+NTTIVSSCLNAKI   GVQNPP CVV
Sbjct: 421 ELRDINLSYGGASLKNTTIVSSCLNAKIRTFGVQNPPACVV 461

[Close]

BLAST of Spg022731 vs. NCBI nr
Match: XP_023549400.1 (exopolygalacturonase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 688.0 bits (1774), Expect = 5.6e-194
Identity = 350/461 (75.92%), Postives = 380/461 (82.43%), Query Frame = 0


Query: 1   MTTSRSFSLFQILLLVFAWQCCAMVVAIFDNEVGIVNLVGGIASTVNQQ-LPTSAPQTLG 60
           MTT+ + SLFQ LLLV A QCCA V A+FD+     N + GI ST NQQ  P + P  LG
Sbjct: 1   MTTTTNLSLFQTLLLVLACQCCAEVAAVFDHVTVAANPIDGIVSTANQQHRPPAPPLPLG 60

Query: 61  IGILTGSSDSVVVNDVKAAVDL--NGKGGAVFDVRKYGAKADGKTDNAQ----------- 120
           IG L  S   V +N  KAAV L  NG G AVFDV+KYGAKA+GKTD+AQ           
Sbjct: 61  IGTLANSRGRVAINGTKAAVYLKGNGNGNAVFDVKKYGAKANGKTDDAQAFMTTWIAACR 120

Query: 121 KTVGPAKFLIPRGTFLVGPVTFAGPCKSLPITIEIQGTVKATTDISEYSSPEWFSIEHIT 180
            T GPAKFLIP+GTFLVGPV FAGPCKS PITIEIQGTVKATTDISEYSSP+W SIE IT
Sbjct: 121 NTTGPAKFLIPKGTFLVGPVVFAGPCKSWPITIEIQGTVKATTDISEYSSPDWISIEGIT 180

Query: 181 GLILTGSDVFDGQGASAWSYNDCKKNIHCQSLPISIKFWKLNHTIVDGITSLNSKAFHTS 240
           GLILTGS VFDGQGASAW YNDCK N +CQ LP SIKF +LNH+IVDG+TS+NSK FHTS
Sbjct: 181 GLILTGSGVFDGQGASAWPYNDCKTNNNCQILPNSIKFTRLNHSIVDGLTSINSKGFHTS 240

Query: 241 VFNCYNFTATNMRIIAPGNSPNTDGMHLSTSKLVTISNSVIGTGDDCVSIGQGCQKITIT 300
           VF CYNFTATNM IIAPGNSPNTDGMHLSTSKLVTIS+S IGTGDDCVSIG  C+KIT+T
Sbjct: 241 VFRCYNFTATNMNIIAPGNSPNTDGMHLSTSKLVTISSSTIGTGDDCVSIGHSCEKITVT 300

Query: 301 NITCGPGHGISVGSLGKYPKEKSVFGVLVQNCTIFNATNGARIKTWADTVSGSATGIIFD 360
           NITCGPGHGIS+GSLG+Y  EKSV  VLVQNCTIFNATNGARIKTWADT+SGSA GIIFD
Sbjct: 301 NITCGPGHGISIGSLGRYKTEKSVSDVLVQNCTIFNATNGARIKTWADTISGSAVGIIFD 360

Query: 361 DIVMYNVKYPIIIDQTYGTKKNKASKWKISDVHFKNIRGTSGTNVAVLLECSALFPCKGV 420
           +IVM  VK PIIIDQTYGTKK KASKWKIS+VHFKNIRGTS TNVAVLLECS LFPC+GV
Sbjct: 361 NIVMRKVKNPIIIDQTYGTKKKKASKWKISNVHFKNIRGTSTTNVAVLLECSELFPCEGV 420

Query: 421 ELRDINLTYGGNNLRNTTIVSSCLNAKIEHHGVQNPPPCVV 448
           ELRDINL+YGG + +NTTIVSSCLNAKI+  GVQNPPPCVV
Sbjct: 421 ELRDINLSYGGASSKNTTIVSSCLNAKIKTFGVQNPPPCVV 461

[Close]

BLAST of Spg022731 vs. NCBI nr
Match: KAG6575809.1 (Exopolygalacturonase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 685.6 bits (1768), Expect = 2.8e-193
Identity = 347/461 (75.27%), Postives = 379/461 (82.21%), Query Frame = 0


Query: 1   MTTSRSFSLFQILLLVFAWQCCAMVVAIFDNEVGIVNLVGGIASTVNQQ-LPTSAPQTLG 60
           MTT+ + SLFQ LLLV A QCCA V A+FD+     N + GI ST NQQ  P + P  LG
Sbjct: 1   MTTTTNLSLFQTLLLVLACQCCAEVAAVFDHVKVAANPIDGIVSTANQQHRPPAPPLPLG 60

Query: 61  IGILTGSSDSVVVNDVKAAVDL--NGKGGAVFDVRKYGAKADGKTDNAQ----------- 120
           IG L  S   V +N  KAAVDL  NG G AVFDV+KYGAKA+GK+D+AQ           
Sbjct: 61  IGTLANSRGRVAINSTKAAVDLKGNGNGNAVFDVKKYGAKANGKSDDAQAFMTTWIAACR 120

Query: 121 KTVGPAKFLIPRGTFLVGPVTFAGPCKSLPITIEIQGTVKATTDISEYSSPEWFSIEHIT 180
            T GPAKFLIP+GTFLVGPV FAGPC+S PITIEIQGTVKATTDISEYSSP+W SIE IT
Sbjct: 121 NTTGPAKFLIPKGTFLVGPVVFAGPCQSRPITIEIQGTVKATTDISEYSSPDWISIEGIT 180

Query: 181 GLILTGSDVFDGQGASAWSYNDCKKNIHCQSLPISIKFWKLNHTIVDGITSLNSKAFHTS 240
           GLILTGS VFDGQGASAW YNDCK N +CQ LP SIKF +LNH+IVDG+TS+NSK FHTS
Sbjct: 181 GLILTGSGVFDGQGASAWPYNDCKTNNNCQILPNSIKFTRLNHSIVDGLTSINSKGFHTS 240

Query: 241 VFNCYNFTATNMRIIAPGNSPNTDGMHLSTSKLVTISNSVIGTGDDCVSIGQGCQKITIT 300
           VF CYNFTATNM IIAPGNSPNTDGMHLSTSKLVTIS+S IGTGDDCVSIG  C+KIT+T
Sbjct: 241 VFRCYNFTATNMNIIAPGNSPNTDGMHLSTSKLVTISSSTIGTGDDCVSIGHSCEKITVT 300

Query: 301 NITCGPGHGISVGSLGKYPKEKSVFGVLVQNCTIFNATNGARIKTWADTVSGSATGIIFD 360
           N+TCGPGHGIS+GSLG+Y  EKSV  VLVQNCTIFNATNG RIKTWADT+SGSA GIIFD
Sbjct: 301 NVTCGPGHGISIGSLGRYKTEKSVLDVLVQNCTIFNATNGVRIKTWADTISGSAVGIIFD 360

Query: 361 DIVMYNVKYPIIIDQTYGTKKNKASKWKISDVHFKNIRGTSGTNVAVLLECSALFPCKGV 420
           +IVM  VK PIIIDQTYGTKK KASKWKIS+VHFKNIRGTS TNVAVLLECS LFPC+GV
Sbjct: 361 NIVMRKVKNPIIIDQTYGTKKKKASKWKISNVHFKNIRGTSTTNVAVLLECSELFPCEGV 420

Query: 421 ELRDINLTYGGNNLRNTTIVSSCLNAKIEHHGVQNPPPCVV 448
           ELRDINL+YGG +L+NTTIVSSCLNAKI   GVQNPP CVV
Sbjct: 421 ELRDINLSYGGASLKNTTIVSSCLNAKIRTFGVQNPPACVV 461

[Close]

BLAST of Spg022731 vs. ExPASy Swiss-Prot
Match: P49062 (Exopolygalacturonase clone GBGE184 OS=Arabidopsis thaliana OX=3702 GN=PGA3 PE=2 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 5.2e-86
Identity = 164/380 (43.16%), Postives = 230/380 (60.53%), Query Frame = 0


Query: 82  NGKGGAVFDVRKYGAKADGKTDNAQKTVG----------PAKFLIPRGTFLVGPVTFAGP 141
           N     V+D+ K+GA  DG T+  +  +           PA  L+P+GTFL GPV FAGP
Sbjct: 45  NSGNPTVYDITKFGAVGDGSTNTFKAFLNTWIQVCDSPVPATLLVPKGTFLAGPVIFAGP 104

Query: 142 CKSLPITIEIQGTVKATTDISEYSSPEWFSIEHITGLILTGSDVFDGQGASAWSYNDCKK 201
           CKS  +T+ + GT+ ATT  S Y++PEWF  E +  L+LTG+  F G+G + W  + C K
Sbjct: 105 CKS-KVTVNVIGTIIATT--SGYATPEWFLFERVDNLVLTGTGTFHGKGEAVWKADGCGK 164

Query: 202 NIHCQSLPISIKFWKLNHTIVDGITSLNSKAFHTSVFNCYNFTATNMRIIAPGNSPNTDG 261
            + C   P S+KF  + +  ++GI+S+N+KAFH  +    N    N+++ AP  SPNTDG
Sbjct: 165 KVQCNLPPTSLKFRNMKNVEINGISSVNAKAFHMFLVKTENVNIQNIKLTAPAESPNTDG 224

Query: 262 MHLSTSKLVTISNSVIGTGDDCVSIGQGCQKITITNITCGPGHGISVGSLGKYPKEKSVF 321
           +HLS +  V+I +S I TGDDCVS+G+G   +T+  + CGPGHG+SVGSLGKY  E+ V 
Sbjct: 225 IHLSNADNVSILDSTIATGDDCVSVGRGSNNVTVERVICGPGHGLSVGSLGKYKNEEDVS 284

Query: 322 GVLVQNCTIFNATNGARIKTWADTVSGSATGIIFDDIVMYNVKYPIIIDQTYGTKKNKAS 381
           G+ V NCT+    NG RIKTW  +    A  I F++I+M +VK PIIIDQ YG++    S
Sbjct: 285 GIHVNNCTMIETDNGLRIKTWGGSDPSKAVDIKFENIIMQSVKNPIIIDQNYGSRGGD-S 344

Query: 382 KWKISDVHFKNIRGTSGTNVAVLLECSALFPCKGVELRDINLTY----GGNNLRNT--TI 441
           +  ISD+ FKNIRGT+ T   V + CS   PC+GV + D+NL Y    GG    ++   +
Sbjct: 345 QVAISDILFKNIRGTTITKDVVQIMCSKSVPCQGVNVVDVNLDYVGKTGGEKKSSSGGLV 404

Query: 442 VSSCLNAKIEHHGVQNPPPC 446
            + C NA +   G  + P C
Sbjct: 405 GALCDNANVIFGGKLSFPMC 420

[Close]

BLAST of Spg022731 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 5.2e-86
Identity = 161/369 (43.63%), Postives = 223/369 (60.43%), Query Frame = 0


Query: 86  GAVFDVRKYGAKADGKTDNAQ--------KTVGPAKFLIPRGTFLVGPVTFAGPCKSLPI 145
           G+VF+V  YGAK  G    A          + GP+  LIP+G + +G V   GPCK   I
Sbjct: 7   GSVFNVNDYGAKGAGDISQAVMKAWKAACASQGPSTVLIPKGNYNMGEVAMQGPCKGSKI 66

Query: 146 TIEIQGTVKATTDISEYSSPEWFSIEHITGLILTGSDVFDGQGASAWSYNDCKKNIHCQS 205
             +I G VKA  D S++ S  W S   I GL ++G+   DGQG +AW+ N+C KN +C+ 
Sbjct: 67  GFQIDGVVKAPADPSKFKSDGWVSFYRIDGLTVSGTGTLDGQGQTAWAKNNCDKNPNCKH 126

Query: 206 LPISIKFWKLNHTIVDGITSLNSKAFHTSVFNCYNFTATNMRIIAPGNSPNTDGMHLSTS 265
             ++++F  L H +V  ITSLNSK FH +V  C + T  ++ + APG S NTDG+H+  S
Sbjct: 127 AAMNLRFDFLKHAMVRDITSLNSKMFHINVLECEDITFQHVTVTAPGTSINTDGIHVGIS 186

Query: 266 KLVTISNSVIGTGDDCVSIGQGCQKITITNITCGPGHGISVGSLGKYPKEKSVFGVLVQN 325
           K VTI+N+ I TGDDC+SIG G Q +TIT + CGPGHGIS+GSLG+Y  EK V G+ V+ 
Sbjct: 187 KGVTITNTKIATGDDCISIGPGSQNVTITQVNCGPGHGISIGSLGRYNNEKEVRGITVKG 246

Query: 326 CTIFNATNGARIKTWADTVSGSATGIIFDDIVMYNVKYPIIIDQTY----GTKKNKASKW 385
           CT     NG R+KTW ++  G+AT + F D+ M NV+ P+I+DQ Y       +   S+ 
Sbjct: 247 CTFSGTMNGVRVKTWPNSPPGAATDLTFQDLTMNNVQNPVILDQEYCPYGQCSRQAPSRI 306

Query: 386 KISDVHFKNIRGTSGTNVAVLLECSALFPCKGVELRDINLTYGGNNLRNTTIVSSCLNAK 443
           K+S+++F NIRGTS   VAV++ CS   PC  +++ +INL+Y G         S+C N K
Sbjct: 307 KLSNINFNNIRGTSTGKVAVVIACSHGMPCSNMKIGEINLSYRG---AGGPATSTCSNVK 366

[Close]

BLAST of Spg022731 vs. ExPASy Swiss-Prot
Match: Q39786 (Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 1.5e-85
Identity = 167/369 (45.26%), Postives = 220/369 (59.62%), Query Frame = 0


Query: 91  VRKYGAKADGKTD----------NAQKTVGPAKFLIPRGTFLVGPVTFAGPCKSLPITIE 150
           V K+GAKADGKTD           A  +V P+  +IP+GT+L+  V   GPCK+ PI I 
Sbjct: 32  VAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVVIPKGTYLLSKVNLEGPCKA-PIEIN 91

Query: 151 IQGTVKATTDISEYSSPEWFSIEHITGLILTGSDVFDGQGASAWSYNDCKKNIHCQSLPI 210
           +QGT++A  D S +  P W     +    + G  +FDGQG+ A+  N C+       LP+
Sbjct: 92  VQGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIFDGQGSIAYEKNTCENREFRSKLPV 151

Query: 211 SIKFWKLNHTIVDGITSLNSKAFHTSVFNCYNFTATNMRIIAPGNSPNTDGMHLSTSKLV 270
           +I+F  L + ++  ITS +SK FH +VF C N T   ++I AP  SPNTDG+H+  S+ V
Sbjct: 152 NIRFDFLTNALIQDITSKDSKLFHINVFACKNITLERLKIEAPDESPNTDGIHMGKSEGV 211

Query: 271 TISNSVIGTGDDCVSIGQGCQKITITNITCGPGHGISVGSLGKYPKEKSVFGVLVQNCTI 330
            I  S I TGDDC+SIG G + + I  ITCGPGHGIS+GSLGK+  E+ V G+ + NCTI
Sbjct: 212 NIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIKISNCTI 271

Query: 331 FNATNGARIKTWADTVSGSATGIIFDDIVMYNVKYPIIIDQTY----GTKKNKASKWKIS 390
            N +NGARIKTW     G+ + I F+DI M NV  PI+IDQ Y      KKN+ SK K+S
Sbjct: 272 TNTSNGARIKTWPGEHGGAVSEIHFEDITMNNVSSPILIDQQYCPWNKCKKNEESKVKLS 331

Query: 391 DVHFKNIRGTSGTNVAVLLECSALFPCKGVELRDINLTYGGNNLRNTTIVSSCLNAKIEH 446
           ++ FKNIRGTS    A+   CS   PC+ VEL DI++ + G         S CLN K   
Sbjct: 332 NISFKNIRGTSALPEAIKFICSGSSPCQNVELADIDIKHNGAE----PATSQCLNVKPIT 391

[Close]

BLAST of Spg022731 vs. ExPASy Swiss-Prot
Match: P35339 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 4.4e-85
Identity = 170/377 (45.09%), Postives = 227/377 (60.21%), Query Frame = 0


Query: 73  NDVKAAVDLNGKGGAVFDVRKYGAKADGKTDNAQKT----------VGPAKFLIPRGTFL 132
           ND KA    +G GG+ FD+ K GA  +GKTD+ +             G    LIP+G FL
Sbjct: 30  NDAKA----SGPGGS-FDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFL 89

Query: 133 VGPVTFAGPCKSLPITIEIQGTVKATTDISEYSS-PEWFSIEHITGLILTGSDVFDGQGA 192
           VGP+ F GPCK   +TI++ G + ATTD+S+Y     W  I  +  L++TG    DGQG 
Sbjct: 90  VGPLNFTGPCKG-DVTIQVNGNLLATTDLSQYKDHGNWIEILRVDNLVITGKGKLDGQGP 149

Query: 193 SAWSYNDCKKNIHCQSLPISIKFWKLNHTIVDGITSLNSKAFHTSVFNCYNFTATNMRII 252
           + WS N C K   C+ LP S+    +N+  V GIT LNSK FH +++ C +    ++ + 
Sbjct: 150 AVWSKNSCVKKYDCKILPNSLVMDFVNNGEVSGITLLNSKFFHMNMYKCKDMLIKDVNVT 209

Query: 253 APGNSPNTDGMHLSTSKLVTISNSVIGTGDDCVSIGQGCQKITITNITCGPGHGISVGSL 312
           APG+SPNTDG+H+  S  VTI+N+VIG GDDC+SIG G  K+ IT +TCGPGHGIS+GSL
Sbjct: 210 APGDSPNTDGIHMGDSSGVTITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSL 269

Query: 313 GKYPKEKSVFGVLVQNCTIFNATNGARIKTWADTVSG-SATGIIFDDIVMYNVKYPIIID 372
           G+Y  EK V  + V++CT+    NG RIK + D  S  +A+ I +++I M +  YPIIID
Sbjct: 270 GRYKDEKDVTDINVKDCTLKKTANGVRIKAYEDAASVLTASKIHYENIKMEDSGYPIIID 329

Query: 373 QTYGTKK----NKASKWKISDVHFKNIRGTSGTNVAVLLECSALFPCKGVELRDINLTYG 432
             Y   K    N ASK  + DV FKNI GTS T  AV L C+A  PC GV + D+N+ Y 
Sbjct: 330 MKYCPNKLCTANGASKVTVKDVTFKNITGTSSTPEAVNLLCTAKIPCTGVTMDDVNIKYS 389

Query: 433 GNNLRNTTIVSSCLNAK 434
           G N +   +   C NAK
Sbjct: 390 GTNNKTMAV---CKNAK 397

[Close]

BLAST of Spg022731 vs. ExPASy Swiss-Prot
Match: Q39766 (Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 5.8e-85
Identity = 166/369 (44.99%), Postives = 220/369 (59.62%), Query Frame = 0


Query: 91  VRKYGAKADGKTD----------NAQKTVGPAKFLIPRGTFLVGPVTFAGPCKSLPITIE 150
           V K+GAKADGKTD           A  +V P+  +IP+GT+L+  V   GPCK+ PI I 
Sbjct: 32  VAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVVIPKGTYLLSKVNLEGPCKA-PIEIN 91

Query: 151 IQGTVKATTDISEYSSPEWFSIEHITGLILTGSDVFDGQGASAWSYNDCKKNIHCQSLPI 210
           +QGT++A  D S +  P W     +    + G  +FDGQG+ A+  N C+       LP+
Sbjct: 92  VQGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIFDGQGSIAYEKNTCENREFRSKLPV 151

Query: 211 SIKFWKLNHTIVDGITSLNSKAFHTSVFNCYNFTATNMRIIAPGNSPNTDGMHLSTSKLV 270
           +I+F  + + ++  ITS +SK FH +VF C N T   ++I AP  SPNTDG+H+  S+ V
Sbjct: 152 NIRFDFVTNALIQDITSKDSKLFHINVFACKNITLERLKIEAPDESPNTDGIHMGKSEGV 211

Query: 271 TISNSVIGTGDDCVSIGQGCQKITITNITCGPGHGISVGSLGKYPKEKSVFGVLVQNCTI 330
            I  S I TGDDC+SIG G + + I  ITCGPGHGIS+GSLGK+  E+ V G+ + NCTI
Sbjct: 212 NIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIKISNCTI 271

Query: 331 FNATNGARIKTWADTVSGSATGIIFDDIVMYNVKYPIIIDQTY----GTKKNKASKWKIS 390
            N +NGARIKTW     G+ + I F+DI M NV  PI+IDQ Y      KKN+ SK K+S
Sbjct: 272 TNTSNGARIKTWPGEHGGAVSEIHFEDITMNNVSSPILIDQQYCPWNKCKKNEESKVKLS 331

Query: 391 DVHFKNIRGTSGTNVAVLLECSALFPCKGVELRDINLTYGGNNLRNTTIVSSCLNAKIEH 446
           ++ FKNIRGTS    A+   CS   PC+ VEL DI++ + G         S CLN K   
Sbjct: 332 NISFKNIRGTSALPEAIKFICSGSSPCQNVELADIDIQHNGAE----PATSQCLNVKPIT 391

[Close]

BLAST of Spg022731 vs. ExPASy TrEMBL
Match: A0A6J1JX85 (exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111488306 PE=3 SV=1)

HSP 1 Score: 688.7 bits (1776), Expect = 1.6e-194
Identity = 345/459 (75.16%), Postives = 381/459 (83.01%), Query Frame = 0


Query: 1   MTTSRSFSLFQILLLVFAWQCCAMVVAIFDNEVGIVNLVGGIASTVNQQ-LPTSAPQTLG 60
           MTT+R+ SLFQ LLLV A QCCA V A+FD+  G  NL+ GI ST NQQ  P +AP  LG
Sbjct: 1   MTTTRNLSLFQTLLLVLACQCCAEVAAVFDHVTGTANLIDGIVSTANQQHRPPAAPLPLG 60

Query: 61  IGILTGSSDSVVVNDVKAAVDLNGKGGAVFDVRKYGAKADGKTDNAQ-----------KT 120
           I        SV +N  KAAV L G G AVFDV+KYGAKA+GK+D+AQ            T
Sbjct: 61  IRTPANRRGSVAINGTKAAVYLKGNGNAVFDVKKYGAKANGKSDDAQAFMTTWIAACRNT 120

Query: 121 VGPAKFLIPRGTFLVGPVTFAGPCKSLPITIEIQGTVKATTDISEYSSPEWFSIEHITGL 180
            GPAKFLIP+G FLVGPVTFAGPCKS+PITIEIQGTVKATTDISEYSSP+W SIE ITGL
Sbjct: 121 TGPAKFLIPKGIFLVGPVTFAGPCKSMPITIEIQGTVKATTDISEYSSPDWISIEGITGL 180

Query: 181 ILTGSDVFDGQGASAWSYNDCKKNIHCQSLPISIKFWKLNHTIVDGITSLNSKAFHTSVF 240
           ILTGS VFDGQGASAW YNDCK N +CQ LP SIKF +LNH+IVDG+TS+NSK FHTSVF
Sbjct: 181 ILTGSGVFDGQGASAWPYNDCKINNNCQILPNSIKFTRLNHSIVDGLTSINSKGFHTSVF 240

Query: 241 NCYNFTATNMRIIAPGNSPNTDGMHLSTSKLVTISNSVIGTGDDCVSIGQGCQKITITNI 300
            CYNFTATNM I+APGNSPNTDGMHLSTSKLVTIS+S IGTGDDCVSIG  C+KIT+TN+
Sbjct: 241 RCYNFTATNMNIMAPGNSPNTDGMHLSTSKLVTISSSTIGTGDDCVSIGHSCEKITVTNV 300

Query: 301 TCGPGHGISVGSLGKYPKEKSVFGVLVQNCTIFNATNGARIKTWADTVSGSATGIIFDDI 360
           TCGPGHGIS+GSLG+Y  EKSV  VLVQNCTIFNATNGARIKTWA+T+SGSA GI+FD+I
Sbjct: 301 TCGPGHGISIGSLGRYKTEKSVLDVLVQNCTIFNATNGARIKTWANTISGSAVGIVFDNI 360

Query: 361 VMYNVKYPIIIDQTYGTKKNKASKWKISDVHFKNIRGTSGTNVAVLLECSALFPCKGVEL 420
           VM  VK PIIIDQTYGTK+ KASKWKISDVHFKNIRGTS TNVAVLLECS LFPC+GVEL
Sbjct: 361 VMRKVKNPIIIDQTYGTKEKKASKWKISDVHFKNIRGTSTTNVAVLLECSELFPCEGVEL 420

Query: 421 RDINLTYGGNNLRNTTIVSSCLNAKIEHHGVQNPPPCVV 448
           RDINL+YGG +L+NTT VSSCLNAKI+  GVQNPP CVV
Sbjct: 421 RDINLSYGGASLKNTTTVSSCLNAKIKTFGVQNPPACVV 459

[Close]

BLAST of Spg022731 vs. ExPASy TrEMBL
Match: A0A6J1GQZ3 (exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111456314 PE=3 SV=1)

HSP 1 Score: 681.4 bits (1757), Expect = 2.5e-192
Identity = 346/461 (75.05%), Postives = 379/461 (82.21%), Query Frame = 0


Query: 1   MTTSRSFSLFQILLLVFAWQCCAMVVAIFDNEVGIVNLVGGIASTVNQQ-LPTSAPQTLG 60
           MTT+ + SLFQ LLLV A QCCA V A+FD+     N + GI ST +QQ  P + P  LG
Sbjct: 1   MTTTTNLSLFQTLLLVLACQCCAEVAAVFDHVKVAANPINGIVSTTSQQHRPPAPPLPLG 60

Query: 61  IGILTGSSDSVVVNDVKAAVDL--NGKGGAVFDVRKYGAKADGKTDNAQ----------- 120
           IG L  S   V +N  KAAVDL  NG G AVFDV+KYGAKA+GK+D+AQ           
Sbjct: 61  IGTLANSHGRVAINSTKAAVDLKGNGNGNAVFDVKKYGAKANGKSDDAQAFMTTWIAACR 120

Query: 121 KTVGPAKFLIPRGTFLVGPVTFAGPCKSLPITIEIQGTVKATTDISEYSSPEWFSIEHIT 180
            T GPAKFLIP+GTFLVGPV FAGPC+S PITIEIQGTVKATTDISEYSSP+W SIE IT
Sbjct: 121 NTTGPAKFLIPKGTFLVGPVVFAGPCQSRPITIEIQGTVKATTDISEYSSPDWISIEGIT 180

Query: 181 GLILTGSDVFDGQGASAWSYNDCKKNIHCQSLPISIKFWKLNHTIVDGITSLNSKAFHTS 240
           GLILTGS VFDGQGASAW YNDCK N +CQ LP SIKF +LNH+IVDG+TS+NSK FHTS
Sbjct: 181 GLILTGSGVFDGQGASAWPYNDCKTNNNCQILPNSIKFTRLNHSIVDGLTSINSKGFHTS 240

Query: 241 VFNCYNFTATNMRIIAPGNSPNTDGMHLSTSKLVTISNSVIGTGDDCVSIGQGCQKITIT 300
           VF CYNFTATNM IIAPGNSPNTDGMHLSTSKLVTIS+S IGTGDDCVSIG  C+KIT+T
Sbjct: 241 VFRCYNFTATNMNIIAPGNSPNTDGMHLSTSKLVTISSSTIGTGDDCVSIGHSCEKITVT 300

Query: 301 NITCGPGHGISVGSLGKYPKEKSVFGVLVQNCTIFNATNGARIKTWADTVSGSATGIIFD 360
           N+TCGPGHGIS+GSLG+Y  EKSV  VLVQNCTIFNATNGARIKTWADT+SGSA GIIFD
Sbjct: 301 NVTCGPGHGISIGSLGRYKTEKSVLDVLVQNCTIFNATNGARIKTWADTISGSAVGIIFD 360

Query: 361 DIVMYNVKYPIIIDQTYGTKKNKASKWKISDVHFKNIRGTSGTNVAVLLECSALFPCKGV 420
           +IVM  VK PIIIDQTYGTKK KASKWKIS+V FKNIRGTS TNVAVLLECS LFPC+GV
Sbjct: 361 NIVMRKVKNPIIIDQTYGTKKKKASKWKISNVLFKNIRGTSTTNVAVLLECSELFPCEGV 420

Query: 421 ELRDINLTYGGNNLRNTTIVSSCLNAKIEHHGVQNPPPCVV 448
           ELRDINL+YGG +L+NTTIVSSCLNAKI   GVQNPP CVV
Sbjct: 421 ELRDINLSYGGASLKNTTIVSSCLNAKIRTFGVQNPPACVV 461

[Close]

BLAST of Spg022731 vs. ExPASy TrEMBL
Match: A0A5A7U4W1 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00950 PE=3 SV=1)

HSP 1 Score: 663.7 bits (1711), Expect = 5.5e-187
Identity = 338/458 (73.80%), Postives = 371/458 (81.00%), Query Frame = 0


Query: 4   SRSFSL-FQILLLVFAWQCCAMVVAIFDNEVGIVNLVGGIASTVNQQL--PTSAPQTLGI 63
           +R+ SL  QILLLVFA QC A V A  D+  G  NL+     TVN+Q   P  AP+ LGI
Sbjct: 2   TRNLSLVIQILLLVFASQCSAKVAATLDDVTGTENLLKVTVPTVNEQFLRPAGAPRRLGI 61

Query: 64  GILTGSSDSVVVNDVKAAVDLNGKGGAVFDVRKYGAKADGKTDNAQ-----------KTV 123
           G L     +  VND++A + LN  GG+VFDV K+GAKADG+TD+AQ            TV
Sbjct: 62  GTLPDIGST--VNDIRANIVLNENGGSVFDVTKHGAKADGETDDAQAFMTTWIAACRNTV 121

Query: 124 GPAKFLIPRGTFLVGPVTFAGPCKSLPITIEIQGTVKATTDISEYSSPEWFSIEHITGLI 183
           GPAKFLIP+GT+LVGPVTFAGPCKS PIT+E QGTVKATTDISEYSSPEWFSIE ITG I
Sbjct: 122 GPAKFLIPQGTYLVGPVTFAGPCKSFPITLENQGTVKATTDISEYSSPEWFSIEDITGFI 181

Query: 184 LTGSDVFDGQGASAWSYNDCKKNIHCQSLPISIKFWKLNHTIVDGITSLNSKAFHTSVFN 243
           LTGS VFDGQG + W YNDCKKN  CQ LPISIKF +LNHTIVDG+TS+NS  FHTSVF 
Sbjct: 182 LTGSGVFDGQGVTVWPYNDCKKNRFCQLLPISIKFSRLNHTIVDGLTSVNSMGFHTSVFY 241

Query: 244 CYNFTATNMRIIAPGNSPNTDGMHLSTSKLVTISNSVIGTGDDCVSIGQGCQKITITNIT 303
           CYNFTATNM+IIAP NSPNTDGMHLSTSKLVTI+NSVIGTGDDCVSIG   + I +TN+T
Sbjct: 242 CYNFTATNMKIIAPHNSPNTDGMHLSTSKLVTITNSVIGTGDDCVSIGHSTENIVVTNVT 301

Query: 304 CGPGHGISVGSLGKYPKEKSVFGVLVQNCTIFNATNGARIKTWADTVSGSATGIIFDDIV 363
           CGPGHG+SVGSLGKY KEKSV+ VLV+NCTIFNATNGARIKTWA  +SG A+GIIF+DIV
Sbjct: 302 CGPGHGLSVGSLGKYSKEKSVYNVLVKNCTIFNATNGARIKTWASRISGLASGIIFEDIV 361

Query: 364 MYNVKYPIIIDQTYGTKKNKASKWKISDVHFKNIRGTSGTNVAVLLECSALFPCKGVELR 423
           MYNVK PIIIDQTYGTKK K S WKISDVHFKNIRGTS TNVAVLLECS L PC+GVELR
Sbjct: 362 MYNVKNPIIIDQTYGTKKKKVSNWKISDVHFKNIRGTSTTNVAVLLECSELLPCEGVELR 421

Query: 424 DINLTYGGNNLRNTTIVSSCLNAKIEHHGVQNPPPCVV 448
           DINLTYGG NLRNTTIVSSC NAKI   GVQNPPPCVV
Sbjct: 422 DINLTYGGTNLRNTTIVSSCSNAKIATFGVQNPPPCVV 457

[Close]

BLAST of Spg022731 vs. ExPASy TrEMBL
Match: A0A5A7TQ89 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36913G00010 PE=3 SV=1)

HSP 1 Score: 661.0 bits (1704), Expect = 3.5e-186
Identity = 337/458 (73.58%), Postives = 370/458 (80.79%), Query Frame = 0


Query: 4   SRSFSL-FQILLLVFAWQCCAMVVAIFDNEVGIVNLVGGIASTVNQQL--PTSAPQTLGI 63
           +R+ SL  QILLLVFA QC A   A  D+  G  NL+     TVN+Q   P  AP+ LGI
Sbjct: 2   TRNLSLVIQILLLVFASQCSAKDAATLDDVTGTENLLKVTVPTVNEQFLRPAGAPRRLGI 61

Query: 64  GILTGSSDSVVVNDVKAAVDLNGKGGAVFDVRKYGAKADGKTDNAQ-----------KTV 123
           G L     +  VND++A + LN  GG+VFDV K+GAKADG+TD+AQ            TV
Sbjct: 62  GTLPDIGST--VNDIRANIVLNENGGSVFDVTKHGAKADGETDDAQAFMTTWIAACRNTV 121

Query: 124 GPAKFLIPRGTFLVGPVTFAGPCKSLPITIEIQGTVKATTDISEYSSPEWFSIEHITGLI 183
           GPAKFLIP+GT+LVGPVTFAGPCKS PIT+E QGTVKATTDISEYSSPEWFSIE ITG I
Sbjct: 122 GPAKFLIPQGTYLVGPVTFAGPCKSFPITLENQGTVKATTDISEYSSPEWFSIEDITGFI 181

Query: 184 LTGSDVFDGQGASAWSYNDCKKNIHCQSLPISIKFWKLNHTIVDGITSLNSKAFHTSVFN 243
           LTGS VFDGQG + W YNDCKKN  CQ LPISIKF +LNHTIVDG+TS+NS  FHTSVF 
Sbjct: 182 LTGSGVFDGQGVTVWPYNDCKKNRFCQLLPISIKFSRLNHTIVDGLTSVNSMGFHTSVFY 241

Query: 244 CYNFTATNMRIIAPGNSPNTDGMHLSTSKLVTISNSVIGTGDDCVSIGQGCQKITITNIT 303
           CYNFTATNM+IIAP NSPNTDGMHLSTSKLVTI+NSVIGTGDDCVSIG   + I +TN+T
Sbjct: 242 CYNFTATNMKIIAPHNSPNTDGMHLSTSKLVTITNSVIGTGDDCVSIGHSTENIVVTNVT 301

Query: 304 CGPGHGISVGSLGKYPKEKSVFGVLVQNCTIFNATNGARIKTWADTVSGSATGIIFDDIV 363
           CGPGHG+SVGSLGKY KEKSV+ VLV+NCTIFNATNGARIKTWA  +SG A+GIIF+DIV
Sbjct: 302 CGPGHGLSVGSLGKYSKEKSVYNVLVKNCTIFNATNGARIKTWASRISGLASGIIFEDIV 361

Query: 364 MYNVKYPIIIDQTYGTKKNKASKWKISDVHFKNIRGTSGTNVAVLLECSALFPCKGVELR 423
           MYNVK PIIIDQTYGTKK K S WKISDVHFKNIRGTS TNVAVLLECS L PC+GVELR
Sbjct: 362 MYNVKNPIIIDQTYGTKKKKVSNWKISDVHFKNIRGTSTTNVAVLLECSELLPCEGVELR 421

Query: 424 DINLTYGGNNLRNTTIVSSCLNAKIEHHGVQNPPPCVV 448
           DINLTYGG NLRNTTIVSSC NAKI   GVQNPPPCVV
Sbjct: 422 DINLTYGGTNLRNTTIVSSCSNAKIATFGVQNPPPCVV 457

[Close]

BLAST of Spg022731 vs. ExPASy TrEMBL
Match: A0A1S3BRD3 (exopolygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103492867 PE=3 SV=1)

HSP 1 Score: 646.4 bits (1666), Expect = 9.0e-182
Identity = 328/460 (71.30%), Postives = 372/460 (80.87%), Query Frame = 0


Query: 1   MTTSRSFSLFQILLLVFAWQCCAMVVAIFDNEVGIVNLVGGIASTVNQQLPT--SAPQTL 60
           MT +R   LFQILL VFAWQCC  V AI ++   I NL   I ST+NQQLP+  +AP  L
Sbjct: 1   MTITRDIGLFQILLWVFAWQCCDKVSAITND---IANLNYEIVSTINQQLPSPVAAPGPL 60

Query: 61  GIGILTGSSDSVVVNDVKAAVDLNGKGGAVFDVRKYGAKADGKTDNAQ-----------K 120
           GI  L    D++V +D+    DLNG GG+VF V K+GAKADGKTD+AQ            
Sbjct: 61  GIETLP-DLDNMVNDDINTNNDLNGNGGSVFHVTKHGAKADGKTDDAQAFVTTWIEACRN 120

Query: 121 TVGPAKFLIPRGTFLVGPVTFAGPCKSLPITIEIQGTVKATTDISEYSSPEWFSIEHITG 180
           TVGPAK LIP GT+LVGPVT AGPCKS PIT+E QGTVKATTDIS+YSSPEWFSIE ITG
Sbjct: 121 TVGPAKILIPEGTYLVGPVTLAGPCKSFPITLENQGTVKATTDISQYSSPEWFSIEDITG 180

Query: 181 LILTGSDVFDGQGASAWSYNDCKKNIHCQSLPISIKFWKLNHTIVDGITSLNSKAFHTSV 240
            ILTGS VFDGQG +AW YNDCK N  CQ LPISIKF +LN TIVD +TSLNSK FH S+
Sbjct: 181 FILTGSGVFDGQGVAAWPYNDCKNNNICQLLPISIKFSRLNDTIVDRLTSLNSKGFHMSL 240

Query: 241 FNCYNFTATNMRIIAPGNSPNTDGMHLSTSKLVTISNSVIGTGDDCVSIGQGCQKITITN 300
           F+CYNFTATN+ IIAP +SPNTDG+HLSTSKLV I+NS+IGTGDDCVSIG   +KIT+TN
Sbjct: 241 FSCYNFTATNINIIAPHDSPNTDGIHLSTSKLVNIANSIIGTGDDCVSIGHSTEKITVTN 300

Query: 301 ITCGPGHGISVGSLGKYPKEKSVFGVLVQNCTIFNATNGARIKTWADTVSGSATGIIFDD 360
           +TCGPGHG+SVGSLGKYP+EK V+ VLV+NCTIFNATNGARIKT+A  +SGSA+GIIF+D
Sbjct: 301 VTCGPGHGLSVGSLGKYPEEKDVYDVLVKNCTIFNATNGARIKTFASPISGSASGIIFED 360

Query: 361 IVMYNVKYPIIIDQTYGTKKNKASKWKISDVHFKNIRGTSGTNVAVLLECSALFPCKGVE 420
           IVMYNVKYPIIIDQTY T +NK SKWK+SDVHFKNIRGTS TNVAVLL+CS L PC+GVE
Sbjct: 361 IVMYNVKYPIIIDQTYDTNENKESKWKVSDVHFKNIRGTSATNVAVLLDCSKLLPCEGVE 420

Query: 421 LRDINLTYGGNNLRNTTIVSSCLNAKIEHHGVQNPPPCVV 448
           LRDI+LTYGG +L+NTTIVSSC NAKI   GVQNPPPC V
Sbjct: 421 LRDIDLTYGGTDLKNTTIVSSCSNAKITTFGVQNPPPCDV 456

[Close]

BLAST of Spg022731 vs. TAIR 10
Match: AT4G18180.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 327.4 bits (838), Expect = 1.8e-89
Identity = 177/381 (46.46%), Postives = 237/381 (62.20%), Query Frame = 0


Query: 90  DVRKYGAKADGKTDNAQKTV-----------GPAKFLIPRGTFLVGPVTFAGPCKSLPIT 149
           DVR +GA+A+   D+ +  V                +IPRG F VG + F+GPC ++   
Sbjct: 36  DVRSFGARANDHRDHTKAFVAAWDKACKSSSSSVNLIIPRGEFSVGSLRFSGPCTNVS-- 95

Query: 150 IEIQGTVKATTDISEY-SSPEWFSIEHITGLILTGSDVFDGQGASAWSYNDCKKNIHCQS 209
             +   VKA+TD+S+Y S   W     I GL LTG   FDGQGA AW +N+C  + +C+ 
Sbjct: 96  -NLTVRVKASTDLSKYRSGGGWIQFGWINGLTLTGGGTFDGQGALAWPFNNCTSDSNCKL 155

Query: 210 LPISIKFWKLNHTIVDGITSLNSKAFHTSVFNCYNFTATNMRIIAPGNSPNTDGMHLSTS 269
           LP S+KF  +N T+V  I+S+NSK FH ++  C +F  T + I AP +SPNTDG+H+  S
Sbjct: 156 LPTSLKFVGMNRTVVRRISSVNSKFFHIALVECRDFKGTRLNITAPSDSPNTDGIHIERS 215

Query: 270 KLVTISNSVIGTGDDCVSIGQGCQKITITNITCGPGHGISVGSLGKYPKEKSVFGVLVQN 329
             V  S S I TGDDCVSIGQG  +ITIT+I CGPGHGISVGSLG+YP EK V G++V++
Sbjct: 216 SNVYFSRSHIATGDDCVSIGQGNSQITITSIKCGPGHGISVGSLGRYPNEKDVNGLVVKD 275

Query: 330 CTIFNATNGARIKTWADTVS-GSATGIIFDDIVMYNVKYPIIIDQTY----GTKKNKASK 389
           C I   TNG RIKTWA++    +AT + F++I+M NV  PIIIDQ+Y        N  SK
Sbjct: 276 CKISGTTNGIRIKTWANSPGLSAATNMTFENIIMNNVTNPIIIDQSYCPFSSCISNVPSK 335

Query: 390 WKISDVHFKNIRGTSGTNVAVLLECSALFPCKGVELRDINL----TYGG----NNLRNTT 446
            ++S+++FKNIRGTS + VAV L CS   PCK V L +++L    + GG    +N  N  
Sbjct: 336 VELSEIYFKNIRGTSSSLVAVQLHCSRGMPCKKVYLENVHLDLSSSDGGRKQSSNRGNEA 395

[Close]

BLAST of Spg022731 vs. TAIR 10
Match: AT1G02790.1 (polygalacturonase 4 )

HSP 1 Score: 319.7 bits (818), Expect = 3.7e-87
Identity = 164/380 (43.16%), Postives = 230/380 (60.53%), Query Frame = 0


Query: 82  NGKGGAVFDVRKYGAKADGKTDNAQKTVG----------PAKFLIPRGTFLVGPVTFAGP 141
           N     V+D+ K+GA  DG T+  +  +           PA  L+P+GTFL GPV FAGP
Sbjct: 45  NSGNPTVYDITKFGAVGDGSTNTFKAFLNTWIQVCDSPVPATLLVPKGTFLAGPVIFAGP 104

Query: 142 CKSLPITIEIQGTVKATTDISEYSSPEWFSIEHITGLILTGSDVFDGQGASAWSYNDCKK 201
           CKS  +T+ + GT+ ATT  S Y++PEWF  E +  L+LTG+  F G+G + W  + C K
Sbjct: 105 CKS-KVTVNVIGTIIATT--SGYATPEWFLFERVDNLVLTGTGTFHGKGEAVWKADGCGK 164

Query: 202 NIHCQSLPISIKFWKLNHTIVDGITSLNSKAFHTSVFNCYNFTATNMRIIAPGNSPNTDG 261
            + C   P S+KF  + +  ++GI+S+N+KAFH  +    N    N+++ AP  SPNTDG
Sbjct: 165 KVQCNLPPTSLKFRNMKNVEINGISSVNAKAFHMFLVKTENVNIQNIKLTAPAESPNTDG 224

Query: 262 MHLSTSKLVTISNSVIGTGDDCVSIGQGCQKITITNITCGPGHGISVGSLGKYPKEKSVF 321
           +HLS +  V+I +S I TGDDCVS+G+G   +T+  + CGPGHG+SVGSLGKY  E+ V 
Sbjct: 225 IHLSNADNVSILDSTIATGDDCVSVGRGSNNVTVERVICGPGHGLSVGSLGKYKNEEDVS 284

Query: 322 GVLVQNCTIFNATNGARIKTWADTVSGSATGIIFDDIVMYNVKYPIIIDQTYGTKKNKAS 381
           G+ V NCT+    NG RIKTW  +    A  I F++I+M +VK PIIIDQ YG++    S
Sbjct: 285 GIHVNNCTMIETDNGLRIKTWGGSDPSKAVDIKFENIIMQSVKNPIIIDQNYGSRGGD-S 344

Query: 382 KWKISDVHFKNIRGTSGTNVAVLLECSALFPCKGVELRDINLTY----GGNNLRNT--TI 441
           +  ISD+ FKNIRGT+ T   V + CS   PC+GV + D+NL Y    GG    ++   +
Sbjct: 345 QVAISDILFKNIRGTTITKDVVQIMCSKSVPCQGVNVVDVNLDYVGKTGGEKKSSSGGLV 404

Query: 442 VSSCLNAKIEHHGVQNPPPC 446
            + C NA +   G  + P C
Sbjct: 405 GALCDNANVIFGGKLSFPMC 420

[Close]

BLAST of Spg022731 vs. TAIR 10
Match: AT3G14040.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 310.5 bits (794), Expect = 2.3e-84
Identity = 167/376 (44.41%), Postives = 221/376 (58.78%), Query Frame = 0


Query: 85  GGAVFDVRKYGAKADGKTDNA--------QKTVGPAKFLIPRGTFLVGPVTFAGPCKSLP 144
           GGA  DV+  GAK DGKTD++        +     +   +P+G +LV  + F GPCK  P
Sbjct: 67  GGASLDVKASGAKGDGKTDDSAAFAAAWKEACAAGSTITVPKGEYLVESLEFKGPCKG-P 126

Query: 145 ITIEIQGTVKATTDISEYSSPE--WFSIEHITGLILTGSD-VFDGQGASAWSYNDCKKNI 204
           +T+E+ G  KA   + + + P   W   E++    L G+  +FDGQG+ AW  NDC K  
Sbjct: 127 VTLELNGNFKAPATV-KTTKPHAGWIDFENLADFTLNGNKAIFDGQGSLAWKANDCAKTG 186

Query: 205 HCQSLPISIKFWKLNHTIVDGITSLNSKAFHTSVFNCYNFTATNMRIIAPGNSPNTDGMH 264
            C SLPI+I+F  L ++ ++ ITS NSK FH ++ NC N T T++ I AP  S NTDG+H
Sbjct: 187 KCNSLPINIRFTGLTNSKINSITSTNSKLFHMNILNCKNITLTDIGIDAPPESLNTDGIH 246

Query: 265 LSTSKLVTISNSVIGTGDDCVSIGQGCQKITITNITCGPGHGISVGSLGKYPKEKSVFGV 324
           +  S  V +  + I TGDDCVSIG G + + + N+ CGPGHGIS+GSLG+YP E+ V GV
Sbjct: 247 IGRSNGVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKGV 306

Query: 325 LVQNCTIFNATNGARIKTWADTVSGSATGIIFDDIVMYNVKYPIIIDQTY----GTKKNK 384
            V+ C I N  NG RIKTW  +  G A+ I+F+DI M NV  P++IDQ Y      K   
Sbjct: 307 TVRKCLIKNTDNGVRIKTWPGSPPGIASNILFEDITMDNVSLPVLIDQEYCPYGHCKAGV 366

Query: 385 ASKWKISDVHFKNIRGTSGTNVAVLLECSALFPCKGVELRDINLTYGGNNLRNTTIVSSC 444
            SK K+SDV  KNI+GTS T VAV L CS   PC  + L DINL + G   +    VS+C
Sbjct: 367 PSKVKLSDVTIKNIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNG---KEGPAVSAC 426

Query: 445 LNAKIEHHGVQNPPPC 446
            N K    G   P  C
Sbjct: 427 SNIKPILSGKLVPAAC 437

[Close]

BLAST of Spg022731 vs. TAIR 10
Match: AT3G07850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 302.0 bits (772), Expect = 8.0e-82
Identity = 163/377 (43.24%), Postives = 219/377 (58.09%), Query Frame = 0


Query: 84  KGGAVFDVRKYGAKADGKTDNA--------QKTVGPAKFLIPRGTFLVGPVTFAGPCKSL 143
           K GA  DV+  GAK D KTD++        +     +   +P+G ++V  + F GPCK  
Sbjct: 65  KPGAAVDVKASGAKGDSKTDDSAAFAAAWKEACAAGSTITVPKGEYMVESLEFKGPCKG- 124

Query: 144 PITIEIQGTVKATTDISEYSSPE--WFSIEHITGLILTGSD-VFDGQGASAWSYNDCKKN 203
           P+T+E+ G  KA   + + + P   W   E+I    L G+  +FDGQG+ AW  NDC K 
Sbjct: 125 PVTLELNGNFKAPATV-KTTKPHAGWIDFENIADFTLNGNKAIFDGQGSLAWKANDCAKT 184

Query: 204 IHCQSLPISIKFWKLNHTIVDGITSLNSKAFHTSVFNCYNFTATNMRIIAPGNSPNTDGM 263
             C SLPI+I+F  L ++ ++ ITS NSK FH ++ NC N T +++ I AP  S NTDG+
Sbjct: 185 GKCNSLPINIRFTGLTNSKINSITSTNSKLFHMNILNCKNITLSDIGIDAPPESLNTDGI 244

Query: 264 HLSTSKLVTISNSVIGTGDDCVSIGQGCQKITITNITCGPGHGISVGSLGKYPKEKSVFG 323
           H+  S  V +  + I TGDDCVSIG G + + + N+ CGPGHGIS+GSLG+YP E+ V G
Sbjct: 245 HIGRSNGVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKG 304

Query: 324 VLVQNCTIFNATNGARIKTWADTVSGSATGIIFDDIVMYNVKYPIIIDQTY----GTKKN 383
           V V+ C I N  NG RIKTW  +  G A+ I+F+DI M NV  P++IDQ Y      K  
Sbjct: 305 VTVRKCLIKNTDNGVRIKTWPGSPPGIASNILFEDITMDNVSLPVLIDQEYCPYGHCKAG 364

Query: 384 KASKWKISDVHFKNIRGTSGTNVAVLLECSALFPCKGVELRDINLTYGGNNLRNTTIVSS 443
             S+ K+SDV  K I+GTS T VAV L CS   PC  + L DINL + G   +    VS+
Sbjct: 365 VPSQVKLSDVTIKGIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNG---KEGPAVSA 424

Query: 444 CLNAKIEHHGVQNPPPC 446
           C N K    G   P  C
Sbjct: 425 CSNIKPILSGKLVPAAC 436

[Close]

BLAST of Spg022731 vs. TAIR 10
Match: AT3G07820.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 288.9 bits (738), Expect = 7.0e-78
Identity = 150/347 (43.23%), Postives = 212/347 (61.10%), Query Frame = 0


Query: 104 NAQKTVGPAKFLIPRGTFLVGPVTFAGPCKSLPITIEIQGTVKATTDISEYSSPEWFSIE 163
           +A ++  P+K +IP+G F +G +   GPCK+ PI + +QGTVKA  +  +    +W    
Sbjct: 45  SACQSSSPSKVVIPKGEFKLGEIEMRGPCKA-PIEVTLQGTVKADGNAIQ-GKEKWVVFG 104

Query: 164 HITGLILTGSDVFDGQGASAWSYNDCKKNIHCQSLPISIKFWKLNHTIVDGITSLNSKAF 223
           +I G  L G   FDG+G +AW  N+C K   C+ LPISI+F  + ++ +  I+S+++K F
Sbjct: 105 NIDGFKLNGGGAFDGEGNAAWRVNNCHKTFECKKLPISIRFDFILNSEIRDISSIDAKNF 164

Query: 224 HTSVFNCYNFTATNMRIIAPGNSPNTDGMHLSTSKLVTISNSVIGTGDDCVSIGQGCQKI 283
           H +V    N T  N++I+AP +SPNTDG+HL  S  V I NS I TGDDC+S+G G + +
Sbjct: 165 HINVLGAKNMTMNNIKIVAPEDSPNTDGIHLGRSDGVKILNSFISTGDDCISVGDGMKNL 224

Query: 284 TITNITCGPGHGISVGSLGKYPKEKSVFGVLVQNCTIFNATNGARIKTWADTV-SGSATG 343
            +  +TCGPGHGISVGSLG+Y  E+ V G+ V NCT+    NG RIKTW     S +A+ 
Sbjct: 225 HVEKVTCGPGHGISVGSLGRYGHEQDVSGIKVINCTLQETDNGLRIKTWPSAACSTTASD 284

Query: 344 IIFDDIVMYNVKYPIIIDQTY----GTKKNKASKWKISDVHFKNIRGTSGTNVAVLLECS 403
           I F+DI++ +V  PI+IDQ Y       K KAS  K+ ++ FKNIRGTSG   AV L CS
Sbjct: 285 IHFEDIILKDVSNPILIDQEYCPWNQCNKQKASTIKLVNISFKNIRGTSGNKDAVKLLCS 344

Query: 404 ALFPCKGVELRDINLTYGGNNLRNTTIVSSCLNAKIEHHGVQNPPPC 446
             +PC+ VE+ DI++ Y G    +      C N   +  G Q+P  C
Sbjct: 345 KGYPCQNVEIGDIDIKYNG---ADGPATFHCSNVSPKILGSQSPKAC 386

The following BLAST results are available for this feature:

BLAST of Spg022731 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038896336.1	1.9e-194	75.11	exopolygalacturonase-like [Benincasa hispida]	[more]
XP_022991773.1	3.3e-194	75.16	exopolygalacturonase-like [Cucurbita maxima]	[more]
KAG7014351.1	4.3e-194	75.70	Exopolygalacturonase-like 4, partial [Cucurbita argyrosperma subsp. argyrosperma...	[more]
XP_023549400.1	5.6e-194	75.92	exopolygalacturonase-like [Cucurbita pepo subsp. pepo]	[more]
KAG6575809.1	2.8e-193	75.27	Exopolygalacturonase, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Spg022731 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P49062	5.2e-86	43.16	Exopolygalacturonase clone GBGE184 OS=Arabidopsis thaliana OX=3702 GN=PGA3 PE=2 ...	[more]
Q6H9K0	5.2e-86	43.63	Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S...	[more]
Q39786	1.5e-85	45.26	Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1	[more]
P35339	4.4e-85	45.09	Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1	[more]
Q39766	5.8e-85	44.99	Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1	[more]

BLAST of Spg022731 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JX85	1.6e-194	75.16	exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111488306 PE=3 SV=1	[more]
A0A6J1GQZ3	2.5e-192	75.05	exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111456314 PE=3 SV=...	[more]
A0A5A7U4W1	5.5e-187	73.80	Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A5A7TQ89	3.5e-186	73.58	Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo...	[more]
A0A1S3BRD3	9.0e-182	71.30	exopolygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103492867 PE=3 SV=1	[more]

BLAST of Spg022731 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G18180.1	1.8e-89	46.46	Pectin lyase-like superfamily protein	[more]
AT1G02790.1	3.7e-87	43.16	polygalacturonase 4	[more]
AT3G14040.1	2.3e-84	44.41	Pectin lyase-like superfamily protein	[more]
AT3G07850.1	8.0e-82	43.24	Pectin lyase-like superfamily protein	[more]
AT3G07820.1	7.0e-78	43.23	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 280..300 e-value: 2300.0 score: 4.6 coord: 340..361 e-value: 4100.0 score: 2.9 coord: 257..278 e-value: 590.0 score: 8.8 coord: 230..256 e-value: 3200.0 score: 3.6 coord: 310..331 e-value: 16.0 score: 14.4
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 110..422 e-value: 5.7E-93 score: 311.5
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 287..300
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 78..444 e-value: 4.4E-99 score: 334.2
None	No IPR available	PANTHER	PTHR31375:SF158	POLYGALACTURONASE	coord: 3..442
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 3..442
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 86..446

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg022731.1	Spg022731.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity