Spg022718 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg022718
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Malic enzyme
Location	scaffold2: 7818515 .. 7823466 (+)
RNA-Seq Expression	Spg022718
Synteny	Spg022718

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAATATCATTTCGTTCTATCCGATAATGGAATCGGCGACCCCACTTTCTCGCTCTCTGTGTCCCGTTTTCTTGTTGGTAGTGCTGCATTTTGATTTTGGCCAAATTTGACTTTGCCTTTTCCACTGTCAATGCTCACAAGTGGCTTTGATTATTTAATGGAAACCCATGTCTGTCCCAATTCGTATTGTGTTCATTTTTGGGGCAGATTTGTTTCTTGCTTCTTCTGCTGCTCTCTTTTTGTGGGGTGTTTCAAATTTGAGTTTAGTGATCGGGGATGGAGAGCACTTTGAAGGAGATCGGTGATGGCGGTTCTGTGCTTGACTTGGACCCTAAATCCACCGTTGGCGGTGGCGTTGAGGACATCTATGGAGAAGACTGTGCTACTGAGGAGCAGCTCGTCACTCCATGGACTTTCTCCGTTGCGAGGTAAATCCAACTTACTTGAACTTTTGGGTTTGGTTTAATAGCGAATTTGTATTGAGTGAGAGATGCAACGGTTTGGTTTGACTGATTACTACAAACTCTCAGTTCATCTTTGTATTCTACTTTGTCCCAACTTTTGGGAACTACTTGCTTTCATTTTGTGATAGTGAATTACCATTAATTCTTGAAGAAGAGTCATTAGGAGGCCTTTGATTCTTCCTTGAGATTCTGATCATGCTGGCCTCTGGATTCTTAGAATATTACTGTTCCCTGTAAATTGGAGAATATTATTCTTAAAATCTAAACGTGAATACTTTTTACACTCTGATATGATGCAGTGGCTATTCCTTGTTGAGAGATCCCCACCATAACAAGGGGTTGGCATTTACAGAGAAGGAAAGAGATGCTCATTATTTGCGGGGACTTTTGCCACCTGCCATTGTTACTCAACAACTTCAGGTTGCCATTGATGTCCCATTTTGAGCTTTTTAGTGGTCATGTTTTTGATGAATTCTTGATGAGCTTTTCCATTTGTGATATCAGGAGAAGAAGTTGATGCAGAACATCAGACAATATCAACTTCCACTACAAAAGTTTATTGCCATGATGGAACTTCAGGTTCTTACTTTACCTACTATTCCTCTGTTTACCTTGGTGATAAGTTTGATGTTTTGAACCTCACTTTTGATTACCTGAATAAGTGAAAAAGATTAAGCTAGTGGCTCAACCATTTTCCTAGGCCCGTTTCATTACTATTTGGTTTTTAGTTTTTGAAAATTAAACTTATAAATATAAGCACTACTCACACTTATGGTTTTCTTTGTTTTGTTATTTACTTTTTACAAATGTTTTCAAAATCTTAGCCAAGTTTTGAAAACTAAAAAAACAGTTTTAAAAAAATCATTCTTGTTTTGGAATTTGACTAAGAATTCAAATCTTTCCTTGAAAAAGATGAAAACTATTTTAAAGAAATTATGAAAAGCATGCATAATTTTCAAAACCTAAAAATAAAAAATAACAAATCAAATGATTATCATACGGACTGTTCGGTTTTCACTGATCATTTTCTTTTATTATTATTTTTTTTTCTGGGTATGATTGGTTGGATTTCTTCTGTTCATATGCCAATTATGATTTATTGGAGTTTGTAATTCGGTTTACAGGAAAGAAATGAAAGGCTTTTTTACAAACTTCTTATCGACAATGTCGAAGAATTGCTTCCTGTTGTCTACACTCCCACAGTTGGTGAAGCTTGCCAGAAGTATGGAAGCATCTTCAGGCGTCCTCAGGGTCTTTACATCAGTTTGAAAGAGAAGTAAAGATGCTTTCCTTTTTTTTTTTGCTCCATGTGTAAGACTTTCAAGGTTCTGTGAATCTTAAAATACCGAATTGTGCAGGGGTAAGATTCTTGAAGTATTGAAGAATTGGCCCCAAAGGAGCATCCAAGTTATTGTTGTGACTGATGGTGAGCGTATTTTGGGTCTTGGTGATCTTGGTTGTCAGGTAATTTCTATGGGAATATTCTCTGATGAGTTAGACCTACGTTACTAGCAGATTTACTGACTTTGCTATGAATTTATTGCACGTTTTAGGGAATGGGAATTCCAGTTGGGAAACTTTCTTTATACACCGCACTTGGAGGAGTTCGTCCTTCTGCAGTAAGGATTTATTTGCAAAGCGCCTGTTCTTTTCTGTCACTACTGTATTTGTACTGCTTCAGTGGAAACTTTCTCCATACACTGCCACATTGAAAATCATCAAACTCATTTAGCTTCATTTAAAGCTTTTAAAGATATTCTTCATTTCAGCTAATACCACATTTTCTTGATAGTGTTTGCCTATTACAATTGATGTCGGGACAAACAATGAGAAATTGTTGAATGATGAATTCTACATTGGGCTTAAACAAAAAAGGGCAACTGGAGAGGTTCGGGACGCTTCTTTATCTTGCTTTTTTCTGGTTTAAGTACAATTGAGATATTTTAAGTTTGTGAGTTTGTAATGAACTCAAGTGTTTTGCCTATGTTCTATGCAGGAGTACTATGAACTTCTACATGAGTTTATGACGGCTGTTAAGCAGAATTATGGGGAAAAAGTTCTTATTCAGGTCAGAACATGATATATATATAGTCATTGGTATCTGAGGTTTGAATCGTTTCTGTACTCAAGGGTTCTAATTGAAACTTTCAGTTTGAAGATTTTGCGAACCACAATGCTTTCGAGCTACTCGCCAAGTATCGTACATCTCATTTAGTCTTCAATGATGATATTCAGGTACTGGATAGTATAAATTTGGTACTCATCATTTAGGAAGTTGTCAAATTTTCTTATCCATAATCTTTTTCTTGTTTCAGGGAACAGCGTCTGTCGTACTTGCTGGAGCTGTTTCTGCCCTGAAACTCATTGGCGGTACCTTGGCTGATCACACTTTCTTGTTTCTTGGTGCTGGGGAAGTAAGATCTCTCCTTCCATGCCTCCATCTCTCTATTTTTGTAAATTCCTTTGTGTAAATGGCCCAGTTTTTCAAAACGCTATCATATTCTTGCATTCCCATACGCCATCCTAGTTCAAAATTTTCTCACATATTTCTTTGGCATGGCTGCAGGCTGGAACTGGTATTGCAGAGCTTATAGCCCTTGAAGTATCAAAACAGGTACTTGTCAAGCTATACCAAATATAAATGGATCACAAACAAATTTCTTGAAATCGATGTTTCCTATGTTTTACCTTCCTTTTTTTCTTTTAGACAAATGCTCCAGTTGAGGAGACCCGCAAGAAGATCTGGCTTGTCGACTCGAAGGTAGTAGAAAGTTTCATCAAGCTTAGGACAGAAGCATAATTATATCTTGATGAGTTAATGTTATTGGCTTCTTGCTTCAACCATAAAGAGAATGATGTTCTTGTAGGGATTAATTGTCCACTCTCGTAAGGAATCACTTCAACACTTCAAGAAGCCATGGGCTCATGAGCATGAACCTGTTAAGGATTTATTAAGTGCTGTCAAGGTGTGAACTATTCTCTTTATGAGTTTCGATTACATCCCGTAATCTCACAATGGTTCCTTGTCTCTTGTCGCTCACATATTTCTCACTATTCCAGGCAATTAAGCCAACAGTTCTGATTGGATCATCTGGAGTTGGAAGGACATTTACGAAAGAAGTCGTTGAGGCTGTGTCCTCCATCAATGAGGTTCTTTCTTTTCCCTCACAGATGGACTCATAAGCACATATACATATTAGATACATGTTTAGAAAAAATTTACAAATAGGATCAACTCAGCCAAACTTATTCCCTGATGCAAACTGACATTAGTTTGTCGCATCGTTACGCAGAAACCTCTTATTATGGCTCTTTCCAACCCGACGTCTCAATCTGAATGCACTGCTGAAGAGGCTTACACTTGGAGTGAGGTACATAACAAACATGACACTTTAAAGCCAAAGTTTTTCTCTCTTTTCTTTTTTCCAGGAACTTTTCTATTTACTTTGCTTTTATATGCAAATTGAAGGGCCGTGCAATCTTCGCTAGTGGAAGTCCATTCGATCCCTTTGAATACAATGGGAAAACCTTTGTCCCTGGCCAGGTTTTTCAACTTTATCATTATGTTAAATGATTTACTCTTCATTACTGTTATCACATATCAAGAGCTGACATTTTGAACCTGTCAAATCTCAAATCAGGCCAACAATGCCTACATTTTCCCTGGTTTCGGTTTGGGTGTCGTAATTTCTGGAGCAATTCGCGTGCACGATGACATGCTTCTGGCTGCCTGTAAGTAGGATTGTTAAATGAGAAATATGACACTTAACTGACTGAAGTACATACATACATGCACAAATATATGCAGACATTTAGTTCTTAACTATTCTTATGATTACAAATGTGGGATCATATCCTCATAACTGAAGTCTAAACACATCATAAATCACAAGTATAGTAACAATGTTAAATCTTTAAATGGCGGTACAATTTATGATTCTTATTTCGCCTTTGTCACACCAGCGGAAGCGTTGGCTGCCCAAGTCTCCGAGGAGAACTACGACAAGGGATTGATTTACCCGCCCTTCACTAACATCCGAAAGATCTCAGCAAACATTGCAGCTAATGTGGCTGCCAAGGCATATGAACTTGGTGAGACATTTTCTTTGATTATGTTGTTATTCTTGTAGTTGTAAGTACTCTTCCATAATTTAAATTCTTTTGCCTCCCATTTATGTACTCTCCAGGCTTGGCGACCCGTCTCCCGCGCCCTGCAGATCTTGTCAAGTATGCTGAGAGCTGCATGTACAGTCCTGTCTACCGAACCTACCGATAAATGTAACGAGATTTCATGGTGCTGGTTTGTATTTTGGAGCTTTTTATGGAAGCTCGGTAATGCATATGGTGTGTGATTAGGAGAGAGCTTGAAGTCTTCCTAAAATCTTTACTTGTAATGATGGAATGTTGTAATGTTTTTCCTTCATAAGTGAACTTATTTTGTTAATGAAATAAGCTTTTGTATCAACTTACAAATGCTTCCATTCCTCC

mRNA sequence

ATGGAGAGCACTTTGAAGGAGATCGGTGATGGCGGTTCTGTGCTTGACTTGGACCCTAAATCCACCGTTGGCGGTGGCGTTGAGGACATCTATGGAGAAGACTGTGCTACTGAGGAGCAGCTCGTCACTCCATGGACTTTCTCCGTTGCGAGTGGCTATTCCTTGTTGAGAGATCCCCACCATAACAAGGGGTTGGCATTTACAGAGAAGGAAAGAGATGCTCATTATTTGCGGGGACTTTTGCCACCTGCCATTGTTACTCAACAACTTCAGGAGAAGAAGTTGATGCAGAACATCAGACAATATCAACTTCCACTACAAAAGTTTATTGCCATGATGGAACTTCAGGAAAGAAATGAAAGGCTTTTTTACAAACTTCTTATCGACAATGTCGAAGAATTGCTTCCTGTTGTCTACACTCCCACAGTTGGTGAAGCTTGCCAGAAGTATGGAAGCATCTTCAGGCGTCCTCAGGGTCTTTACATCAGTTTGAAAGAGAAGGGTAAGATTCTTGAAGTATTGAAGAATTGGCCCCAAAGGAGCATCCAAGTTATTGTTGTGACTGATGGTGAGCGTATTTTGGGTCTTGGTGATCTTGGTTGTCAGTGTTTGCCTATTACAATTGATGTCGGGACAAACAATGAGAAATTGTTGAATGATGAATTCTACATTGGGCTTAAACAAAAAAGGGCAACTGGAGAGGAGTACTATGAACTTCTACATGAGTTTATGACGGCTGTTAAGCAGAATTATGGGGAAAAAGTTCTTATTCAGTTTGAAGATTTTGCGAACCACAATGCTTTCGAGCTACTCGCCAAGTATCGTACATCTCATTTAGTCTTCAATGATGATATTCAGGGAACAGCGTCTGTCGTACTTGCTGGAGCTGTTTCTGCCCTGAAACTCATTGGCGGTACCTTGGCTGATCACACTTTCTTGTTTCTTGGTGCTGGGGAAGCTGGAACTGGTATTGCAGAGCTTATAGCCCTTGAAGTATCAAAACAGACAAATGCTCCAGTTGAGGAGACCCGCAAGAAGATCTGGCTTGTCGACTCGAAGGGATTAATTGTCCACTCTCGTAAGGAATCACTTCAACACTTCAAGAAGCCATGGGCTCATGAGCATGAACCTGTTAAGGATTTATTAAGTGCTGTCAAGGCAATTAAGCCAACAGTTCTGATTGGATCATCTGGAGTTGGAAGGACATTTACGAAAGAAGTCGTTGAGGCTGTGTCCTCCATCAATGAGAAACCTCTTATTATGGCTCTTTCCAACCCGACGTCTCAATCTGAATGCACTGCTGAAGAGGCTTACACTTGGAGTGAGGGCCGTGCAATCTTCGCTAGTGGAAGTCCATTCGATCCCTTTGAATACAATGGGAAAACCTTTGTCCCTGGCCAGGCCAACAATGCCTACATTTTCCCTGGTTTCGGTTTGGGTGTCGTAATTTCTGGAGCAATTCGCGTGCACGATGACATGCTTCTGGCTGCCTCGGAAGCGTTGGCTGCCCAAGTCTCCGAGGAGAACTACGACAAGGGATTGATTTACCCGCCCTTCACTAACATCCGAAAGATCTCAGCAAACATTGCAGCTAATGTGGCTGCCAAGGCATATGAACTTGGCTTGGCGACCCGTCTCCCGCGCCCTGCAGATCTTGTCAAGTATGCTGAGAGCTGCATGTACAGTCCTGTCTACCGAACCTACCGATAA

Coding sequence (CDS)

ATGGAGAGCACTTTGAAGGAGATCGGTGATGGCGGTTCTGTGCTTGACTTGGACCCTAAATCCACCGTTGGCGGTGGCGTTGAGGACATCTATGGAGAAGACTGTGCTACTGAGGAGCAGCTCGTCACTCCATGGACTTTCTCCGTTGCGAGTGGCTATTCCTTGTTGAGAGATCCCCACCATAACAAGGGGTTGGCATTTACAGAGAAGGAAAGAGATGCTCATTATTTGCGGGGACTTTTGCCACCTGCCATTGTTACTCAACAACTTCAGGAGAAGAAGTTGATGCAGAACATCAGACAATATCAACTTCCACTACAAAAGTTTATTGCCATGATGGAACTTCAGGAAAGAAATGAAAGGCTTTTTTACAAACTTCTTATCGACAATGTCGAAGAATTGCTTCCTGTTGTCTACACTCCCACAGTTGGTGAAGCTTGCCAGAAGTATGGAAGCATCTTCAGGCGTCCTCAGGGTCTTTACATCAGTTTGAAAGAGAAGGGTAAGATTCTTGAAGTATTGAAGAATTGGCCCCAAAGGAGCATCCAAGTTATTGTTGTGACTGATGGTGAGCGTATTTTGGGTCTTGGTGATCTTGGTTGTCAGTGTTTGCCTATTACAATTGATGTCGGGACAAACAATGAGAAATTGTTGAATGATGAATTCTACATTGGGCTTAAACAAAAAAGGGCAACTGGAGAGGAGTACTATGAACTTCTACATGAGTTTATGACGGCTGTTAAGCAGAATTATGGGGAAAAAGTTCTTATTCAGTTTGAAGATTTTGCGAACCACAATGCTTTCGAGCTACTCGCCAAGTATCGTACATCTCATTTAGTCTTCAATGATGATATTCAGGGAACAGCGTCTGTCGTACTTGCTGGAGCTGTTTCTGCCCTGAAACTCATTGGCGGTACCTTGGCTGATCACACTTTCTTGTTTCTTGGTGCTGGGGAAGCTGGAACTGGTATTGCAGAGCTTATAGCCCTTGAAGTATCAAAACAGACAAATGCTCCAGTTGAGGAGACCCGCAAGAAGATCTGGCTTGTCGACTCGAAGGGATTAATTGTCCACTCTCGTAAGGAATCACTTCAACACTTCAAGAAGCCATGGGCTCATGAGCATGAACCTGTTAAGGATTTATTAAGTGCTGTCAAGGCAATTAAGCCAACAGTTCTGATTGGATCATCTGGAGTTGGAAGGACATTTACGAAAGAAGTCGTTGAGGCTGTGTCCTCCATCAATGAGAAACCTCTTATTATGGCTCTTTCCAACCCGACGTCTCAATCTGAATGCACTGCTGAAGAGGCTTACACTTGGAGTGAGGGCCGTGCAATCTTCGCTAGTGGAAGTCCATTCGATCCCTTTGAATACAATGGGAAAACCTTTGTCCCTGGCCAGGCCAACAATGCCTACATTTTCCCTGGTTTCGGTTTGGGTGTCGTAATTTCTGGAGCAATTCGCGTGCACGATGACATGCTTCTGGCTGCCTCGGAAGCGTTGGCTGCCCAAGTCTCCGAGGAGAACTACGACAAGGGATTGATTTACCCGCCCTTCACTAACATCCGAAAGATCTCAGCAAACATTGCAGCTAATGTGGCTGCCAAGGCATATGAACTTGGCTTGGCGACCCGTCTCCCGCGCCCTGCAGATCTTGTCAAGTATGCTGAGAGCTGCATGTACAGTCCTGTCTACCGAACCTACCGATAA

Protein sequence

MESTLKEIGDGGSVLDLDPKSTVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPHHNKGLAFTEKERDAHYLRGLLPPAIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQRSIQVIVVTDGERILGLGDLGCQCLPITIDVGTNNEKLLNDEFYIGLKQKRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTNAPVEETRKKIWLVDSKGLIVHSRKESLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEYNGKTFVPGQANNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPPFTNIRKISANIAANVAAKAYELGLATRLPRPADLVKYAESCMYSPVYRTYR

Homology

[Close]

BLAST of Spg022718 vs. NCBI nr
Match: XP_004135668.1 (NADP-dependent malic enzyme [Cucumis sativus] >KGN66170.1 hypothetical protein Csa_007288 [Cucumis sativus])

HSP 1 Score: 1103.6 bits (2853), Expect = 0.0e+00
Identity = 560/591 (94.75%), Postives = 567/591 (95.94%), Query Frame = 0


Query: 1   MESTLKEIGDGGSVLDLDPKSTVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH 60
           MESTLKEIGDGGSVLDLDPK+TVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH
Sbjct: 1   MESTLKEIGDGGSVLDLDPKATVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH 60

Query: 61  HNKGLAFTEKERDAHYLRGLLPPAIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120
           HNKGLAFTEKERDAHYLRGLLPPAIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE
Sbjct: 61  HNKGLAFTEKERDAHYLRGLLPPAIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120

Query: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180
           RLFYKLL+DNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR
Sbjct: 121 RLFYKLLVDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180

Query: 181 SIQVIVVTDGERILGLGDLGCQ----------------------CLPITIDVGTNNEKLL 240
           SIQVIVVTDGERILGLGDLGCQ                      CLPITIDVGTNNEKLL
Sbjct: 181 SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240

Query: 241 NDEFYIGLKQKRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSH 300
           NDEFYIGLKQ+RATGEEYYELL EFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRT+H
Sbjct: 241 NDEFYIGLKQRRATGEEYYELLDEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTTH 300

Query: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTNA 360
           LVFNDDIQGTA+VVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEVSKQT A
Sbjct: 301 LVFNDDIQGTAAVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTKA 360

Query: 361 PVEETRKKIWLVDSKGLIVHSRKESLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSG 420
           PVEETRKKIWLVDSKGLIVHSRK+SLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSG
Sbjct: 361 PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSG 420

Query: 421 VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY 480
           VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY
Sbjct: 421 VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY 480

Query: 481 NGKTFVPGQANNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPP 540
           NGKTFVPGQ+NNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPP
Sbjct: 481 NGKTFVPGQSNNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPP 540

Query: 541 FTNIRKISANIAANVAAKAYELGLATRLPRPADLVKYAESCMYSPVYRTYR 570
           FTNIRKISANIAANVAAKAYELGLATRLPRPADLVKYAESCMYSPVYRTYR
Sbjct: 541 FTNIRKISANIAANVAAKAYELGLATRLPRPADLVKYAESCMYSPVYRTYR 591

[Close]

BLAST of Spg022718 vs. NCBI nr
Match: XP_008450776.1 (PREDICTED: NADP-dependent malic enzyme [Cucumis melo] >TYK10154.1 NADP-dependent malic enzyme [Cucumis melo var. makuwa])

HSP 1 Score: 1101.7 bits (2848), Expect = 0.0e+00
Identity = 558/591 (94.42%), Postives = 567/591 (95.94%), Query Frame = 0


Query: 1   MESTLKEIGDGGSVLDLDPKSTVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH 60
           MESTLKEIGDGGSVLDLDPK+TVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH
Sbjct: 1   MESTLKEIGDGGSVLDLDPKATVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH 60

Query: 61  HNKGLAFTEKERDAHYLRGLLPPAIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120
           HNKGLAFTEKERDAHYLRGLLPPAIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE
Sbjct: 61  HNKGLAFTEKERDAHYLRGLLPPAIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120

Query: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180
           RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR
Sbjct: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180

Query: 181 SIQVIVVTDGERILGLGDLGCQ----------------------CLPITIDVGTNNEKLL 240
           SIQVIVVTDGERILGLGDLGCQ                      CLPITIDVGTNNEKLL
Sbjct: 181 SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240

Query: 241 NDEFYIGLKQKRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSH 300
           NDEFYIGLKQ+RATGEEYYELL EFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRT+H
Sbjct: 241 NDEFYIGLKQRRATGEEYYELLDEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTTH 300

Query: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTNA 360
           LVFNDDIQGTA+VVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALE+SKQT A
Sbjct: 301 LVFNDDIQGTAAVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTKA 360

Query: 361 PVEETRKKIWLVDSKGLIVHSRKESLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSG 420
           PVEETRKKIWLVDSKGLIVHSRK+SLQHFK+PWAHEHEPVKDLLSAVKAIKPTVLIGSSG
Sbjct: 361 PVEETRKKIWLVDSKGLIVHSRKDSLQHFKQPWAHEHEPVKDLLSAVKAIKPTVLIGSSG 420

Query: 421 VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY 480
           VGRTFTKEV+EAVSSINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY
Sbjct: 421 VGRTFTKEVIEAVSSINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY 480

Query: 481 NGKTFVPGQANNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPP 540
           NGKTFVPGQ+NNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPP
Sbjct: 481 NGKTFVPGQSNNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPP 540

Query: 541 FTNIRKISANIAANVAAKAYELGLATRLPRPADLVKYAESCMYSPVYRTYR 570
           FTNIRKISANIAANVAAKAYELGLATRLPRPADLVKYAESCMYSPVYRTYR
Sbjct: 541 FTNIRKISANIAANVAAKAYELGLATRLPRPADLVKYAESCMYSPVYRTYR 591

[Close]

BLAST of Spg022718 vs. NCBI nr
Match: KAG6589949.1 (hypothetical protein SDJN03_15372, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1100.9 bits (2846), Expect = 0.0e+00
Identity = 555/591 (93.91%), Postives = 567/591 (95.94%), Query Frame = 0


Query: 1   MESTLKEIGDGGSVLDLDPKSTVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH 60
           MESTLKEIGDGGSVLDLDPK TVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH
Sbjct: 1   MESTLKEIGDGGSVLDLDPKGTVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH 60

Query: 61  HNKGLAFTEKERDAHYLRGLLPPAIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120
           HNKGLAFTEKERDAHYLRGLLPP+IVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE
Sbjct: 61  HNKGLAFTEKERDAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120

Query: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180
           RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR
Sbjct: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180

Query: 181 SIQVIVVTDGERILGLGDLGCQ----------------------CLPITIDVGTNNEKLL 240
           SIQVIVVTDGERILGLGDLGCQ                      CLPITIDVGTNNEKLL
Sbjct: 181 SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240

Query: 241 NDEFYIGLKQKRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSH 300
           NDEFYIGLKQ+RATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRT+H
Sbjct: 241 NDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTTH 300

Query: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTNA 360
           LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALE+SKQTNA
Sbjct: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTNA 360

Query: 361 PVEETRKKIWLVDSKGLIVHSRKESLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSG 420
           PVEETRKKIWLVDSKGLIVHSRK+SLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSG
Sbjct: 361 PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSG 420

Query: 421 VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY 480
           VGRTFTKEV+EAVS+INEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY
Sbjct: 421 VGRTFTKEVIEAVSAINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY 480

Query: 481 NGKTFVPGQANNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPP 540
           NGKTFVPGQ+NNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAA+VS+ENY+KGLIYPP
Sbjct: 481 NGKTFVPGQSNNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAEVSDENYEKGLIYPP 540

Query: 541 FTNIRKISANIAANVAAKAYELGLATRLPRPADLVKYAESCMYSPVYRTYR 570
           FTNIRKISANIAA VAAKAYELGLATRLPRPADLVKYAESCMYSP+YRTYR
Sbjct: 541 FTNIRKISANIAAKVAAKAYELGLATRLPRPADLVKYAESCMYSPIYRTYR 591

[Close]

BLAST of Spg022718 vs. NCBI nr
Match: XP_023516304.1 (NADP-dependent malic enzyme [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1099.3 bits (2842), Expect = 0.0e+00
Identity = 554/591 (93.74%), Postives = 567/591 (95.94%), Query Frame = 0


Query: 1   MESTLKEIGDGGSVLDLDPKSTVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH 60
           MESTLKEIGDGGSVLDLDPK TVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH
Sbjct: 1   MESTLKEIGDGGSVLDLDPKGTVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH 60

Query: 61  HNKGLAFTEKERDAHYLRGLLPPAIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120
           HNKGLAFTEKERDAHYLRGLLPP+IV+QQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE
Sbjct: 61  HNKGLAFTEKERDAHYLRGLLPPSIVSQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120

Query: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180
           RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR
Sbjct: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180

Query: 181 SIQVIVVTDGERILGLGDLGCQ----------------------CLPITIDVGTNNEKLL 240
           SIQVIVVTDGERILGLGDLGCQ                      CLPITIDVGTNNEKLL
Sbjct: 181 SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240

Query: 241 NDEFYIGLKQKRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSH 300
           NDEFYIGLKQ+RATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRT+H
Sbjct: 241 NDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTTH 300

Query: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTNA 360
           LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALE+SKQTNA
Sbjct: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTNA 360

Query: 361 PVEETRKKIWLVDSKGLIVHSRKESLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSG 420
           PVEETRKKIWLVDSKGLIVHSRK+SLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSG
Sbjct: 361 PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSG 420

Query: 421 VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY 480
           VGRTFTKEV+EAVS+INEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY
Sbjct: 421 VGRTFTKEVIEAVSAINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY 480

Query: 481 NGKTFVPGQANNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPP 540
           NGKTFVPGQ+NNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAA+VS+ENY+KGLIYPP
Sbjct: 481 NGKTFVPGQSNNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAEVSDENYEKGLIYPP 540

Query: 541 FTNIRKISANIAANVAAKAYELGLATRLPRPADLVKYAESCMYSPVYRTYR 570
           FTNIRKISANIAA VAAKAYELGLATRLPRPADLVKYAESCMYSP+YRTYR
Sbjct: 541 FTNIRKISANIAAKVAAKAYELGLATRLPRPADLVKYAESCMYSPIYRTYR 591

[Close]

BLAST of Spg022718 vs. NCBI nr
Match: XP_022961041.1 (NADP-dependent malic enzyme [Cucurbita moschata])

HSP 1 Score: 1099.0 bits (2841), Expect = 0.0e+00
Identity = 554/591 (93.74%), Postives = 566/591 (95.77%), Query Frame = 0


Query: 1   MESTLKEIGDGGSVLDLDPKSTVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH 60
           MESTLKEIGDGGSVLDLDPK TVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH
Sbjct: 1   MESTLKEIGDGGSVLDLDPKGTVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH 60

Query: 61  HNKGLAFTEKERDAHYLRGLLPPAIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120
           HNKGLAFTEKERDAHYLRGLLPP+IVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE
Sbjct: 61  HNKGLAFTEKERDAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120

Query: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180
           RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR
Sbjct: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180

Query: 181 SIQVIVVTDGERILGLGDLGCQ----------------------CLPITIDVGTNNEKLL 240
           SIQVIVVTDGERILGLGDLGCQ                      CLPITIDVGTNNEKLL
Sbjct: 181 SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240

Query: 241 NDEFYIGLKQKRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSH 300
           NDEFYIGLKQ+RATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRT+H
Sbjct: 241 NDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTTH 300

Query: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTNA 360
           LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALE+SKQTNA
Sbjct: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTNA 360

Query: 361 PVEETRKKIWLVDSKGLIVHSRKESLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSG 420
           PVEETRKKIWLVDSKGLIVHSRK+SLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSG
Sbjct: 361 PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSG 420

Query: 421 VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY 480
           VGRTFTKEV+EAVS+INEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY
Sbjct: 421 VGRTFTKEVIEAVSAINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY 480

Query: 481 NGKTFVPGQANNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPP 540
           NGKTFVPGQ+NNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAA+VS+ENY+ GLIYPP
Sbjct: 481 NGKTFVPGQSNNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAEVSDENYENGLIYPP 540

Query: 541 FTNIRKISANIAANVAAKAYELGLATRLPRPADLVKYAESCMYSPVYRTYR 570
           FTNIRKISANIAA VAAKAYELGLATRLPRPADLVKYAESCMYSP+YRTYR
Sbjct: 541 FTNIRKISANIAAKVAAKAYELGLATRLPRPADLVKYAESCMYSPIYRTYR 591

[Close]

BLAST of Spg022718 vs. ExPASy Swiss-Prot
Match: P51615 (NADP-dependent malic enzyme OS=Vitis vinifera OX=29760 PE=2 SV=1)

HSP 1 Score: 988.4 bits (2554), Expect = 3.3e-287
Identity = 494/591 (83.59%), Postives = 537/591 (90.86%), Query Frame = 0


Query: 1   MESTLKEIGDGGSVLDLDPKSTVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH 60
           MESTLK+I DG SVLDLDPK+TVGGGVED+YGED ATE+QLVTPWT SVASGYSLLRDP 
Sbjct: 1   MESTLKDIRDGASVLDLDPKATVGGGVEDLYGEDFATEDQLVTPWTVSVASGYSLLRDPR 60

Query: 61  HNKGLAFTEKERDAHYLRGLLPPAIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120
           HNKGLAF +KERDAHYL GLLPP + TQ+LQE+KLM +IRQYQ+PLQK++AMM+LQERNE
Sbjct: 61  HNKGLAFNDKERDAHYLCGLLPPVVSTQELQERKLMNSIRQYQVPLQKYMAMMDLQERNE 120

Query: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180
           RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWP+R
Sbjct: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPER 180

Query: 181 SIQVIVVTDGERILGLGDLGCQ----------------------CLPITIDVGTNNEKLL 240
            IQVIVVTDGERILGLGDLGCQ                      CLPITIDVGTNNEKLL
Sbjct: 181 RIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240

Query: 241 NDEFYIGLKQKRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSH 300
            +EFYIGLKQ+RATG+EY E L EFM+ VKQNYGEKVLIQFEDFANHNAF+LLAKY T+H
Sbjct: 241 ANEFYIGLKQRRATGKEYSEFLQEFMSPVKQNYGEKVLIQFEDFANHNAFDLLAKYGTTH 300

Query: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTNA 360
           L FNDDIQGTASVVLAG VSAL+L+GGTLADH FLFLGAGEAGTGIAELIALE+SKQT  
Sbjct: 301 LAFNDDIQGTASVVLAGIVSALRLLGGTLADHKFLFLGAGEAGTGIAELIALEMSKQTKC 360

Query: 361 PVEETRKKIWLVDSKGLIVHSRKESLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSG 420
           P+EETRKKIWLVDSKGLIV SRK+SLQ FKKPWAHEHEPVKDLL AVK IKPTVLIGSSG
Sbjct: 361 PIEETRKKIWLVDSKGLIVGSRKDSLQQFKKPWAHEHEPVKDLLDAVKVIKPTVLIGSSG 420

Query: 421 VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY 480
           VG+ FTKEV+EA++S NEKPLI+ALSNPTSQSECTAEEAYTW++GRAIFASGSPFDP EY
Sbjct: 421 VGKAFTKEVIEAMASCNEKPLILALSNPTSQSECTAEEAYTWTQGRAIFASGSPFDPVEY 480

Query: 481 NGKTFVPGQANNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPP 540
           NGKTFVPGQANNAYIFPG G+G+VISGAIRVHD+MLLAASEALA QV++EN+DKGLIYPP
Sbjct: 481 NGKTFVPGQANNAYIFPGLGMGLVISGAIRVHDEMLLAASEALARQVTQENFDKGLIYPP 540

Query: 541 FTNIRKISANIAANVAAKAYELGLATRLPRPADLVKYAESCMYSPVYRTYR 570
           F+NIRKISA+IAANVAAKAYELGLATRLP+P +LVKYAESCMYSPVYR+YR
Sbjct: 541 FSNIRKISAHIAANVAAKAYELGLATRLPQPENLVKYAESCMYSPVYRSYR 591

[Close]

BLAST of Spg022718 vs. ExPASy Swiss-Prot
Match: P34105 (NADP-dependent malic enzyme OS=Populus trichocarpa OX=3694 PE=2 SV=3)

HSP 1 Score: 980.7 bits (2534), Expect = 7.0e-285
Identity = 486/591 (82.23%), Postives = 532/591 (90.02%), Query Frame = 0


Query: 1   MESTLKEIGDGGSVLDLDPKSTVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH 60
           MESTLKE+ DG SVLD+DPKSTVGGGVED+YGED ATE+QLVTPWT SVASGY+LLRDPH
Sbjct: 1   MESTLKEMRDGASVLDMDPKSTVGGGVEDVYGEDRATEDQLVTPWTISVASGYTLLRDPH 60

Query: 61  HNKGLAFTEKERDAHYLRGLLPPAIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120
           HNKGLAFTEKERDAHYLRGLLPP  ++QQLQEKKLM  IRQYQLPLQK+ AMMEL+ERNE
Sbjct: 61  HNKGLAFTEKERDAHYLRGLLPPTTISQQLQEKKLMNTIRQYQLPLQKYTAMMELEERNE 120

Query: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180
           RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIF+RPQGLYISLKEKGK+L+VLKNWPQ+
Sbjct: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFKRPQGLYISLKEKGKVLDVLKNWPQK 180

Query: 181 SIQVIVVTDGERILGLGDLGCQ----------------------CLPITIDVGTNNEKLL 240
           SIQVIVVTDGERILGLGDLGCQ                      CLP+TIDVGTNNE+LL
Sbjct: 181 SIQVIVVTDGERILGLGDLGCQGIGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEQLL 240

Query: 241 NDEFYIGLKQKRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSH 300
            DEFYIGL+Q+RATG+EY ELLHEFMTAVKQNYGEKVLIQFEDFANHNAF+LLAKY T+H
Sbjct: 241 KDEFYIGLRQRRATGQEYSELLHEFMTAVKQNYGEKVLIQFEDFANHNAFDLLAKYGTTH 300

Query: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTNA 360
           LVFNDDIQGTA+VVLAG +SALKL+GG+LADHTFLFLGAGEAGTGIAELIALE+S+++  
Sbjct: 301 LVFNDDIQGTAAVVLAGLISALKLLGGSLADHTFLFLGAGEAGTGIAELIALEMSRRSKT 360

Query: 361 PVEETRKKIWLVDSKGLIVHSRKESLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSG 420
           P+EETRKKIWL DSKGLIV SRKESLQHFKKPWAHEHEPVK LL  VKAIKP VLIG+SG
Sbjct: 361 PLEETRKKIWLTDSKGLIVSSRKESLQHFKKPWAHEHEPVKGLLEVVKAIKPIVLIGTSG 420

Query: 421 VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY 480
           VG+TFTKEV+EA++S NEKPLI+ALSNPTSQSECTA+EAYTW++G+AIFASGSPFDP EY
Sbjct: 421 VGKTFTKEVIEAMASFNEKPLILALSNPTSQSECTAQEAYTWTKGKAIFASGSPFDPVEY 480

Query: 481 NGKTFVPGQANNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPP 540
            GK FVPGQ+NNAYIFPG GLG+VISGAIRVHDDMLLAA+EALA Q+ EE   KGLIYPP
Sbjct: 481 EGKVFVPGQSNNAYIFPGLGLGLVISGAIRVHDDMLLAAAEALAGQIKEEYLAKGLIYPP 540

Query: 541 FTNIRKISANIAANVAAKAYELGLATRLPRPADLVKYAESCMYSPVYRTYR 570
            +NIRKIS  IAANVAAKAYELGLATRLPRP +LVK+AESCMYSP YR YR
Sbjct: 541 LSNIRKISVQIAANVAAKAYELGLATRLPRPENLVKHAESCMYSPAYRYYR 591

[Close]

BLAST of Spg022718 vs. ExPASy Swiss-Prot
Match: P12628 (NADP-dependent malic enzyme OS=Phaseolus vulgaris OX=3885 GN=ME1 PE=2 SV=1)

HSP 1 Score: 938.3 bits (2424), Expect = 4.0e-272
Identity = 468/582 (80.41%), Postives = 515/582 (88.49%), Query Frame = 0


Query: 10  DGGSVLDLDPKSTVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPHHNKGLAFTE 69
           + G  + +    + GGGV D+YGED ATE+ L+TPWTFSVASG SLLRDP +NKGLAFTE
Sbjct: 8   ENGGEVSVKKDYSNGGGVRDLYGEDSATEDHLITPWTFSVASGCSLLRDPRYNKGLAFTE 67

Query: 70  KERDAHYLRGLLPPAIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNERLFYKLLID 129
            ERDAHYLRGLLPP++  Q+LQEK+LM N+RQY++PL +++A+M+LQERNERLFYKLLID
Sbjct: 68  GERDAHYLRGLLPPSVFNQELQEKRLMHNLRQYEVPLHRYMALMDLQERNERLFYKLLID 127

Query: 130 NVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQRSIQVIVVTD 189
           NV ELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWP++SIQVIVVTD
Sbjct: 128 NVAELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPEKSIQVIVVTD 187

Query: 190 GERILGLGDLGCQ----------------------CLPITIDVGTNNEKLLNDEFYIGLK 249
           GERILGLGDLGCQ                      CLP+TIDVGTNNEKLLNDEFYIGL+
Sbjct: 188 GERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSSCLPVTIDVGTNNEKLLNDEFYIGLR 247

Query: 250 QKRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQG 309
           Q+RATG+EY   L EFM AVKQNYGEKVL+QFEDFANHNAF+LL KY +SHLVFNDDIQG
Sbjct: 248 QRRATGQEYATFLDEFMRAVKQNYGEKVLVQFEDFANHNAFDLLEKYSSSHLVFNDDIQG 307

Query: 310 TASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTNAPVEETRKKI 369
           TASVVLAG +++LKL+GGTLADHTFLFLGAGEAGTGIAELIA+EVSKQT APVEETRKKI
Sbjct: 308 TASVVLAGLLASLKLVGGTLADHTFLFLGAGEAGTGIAELIAVEVSKQTKAPVEETRKKI 367

Query: 370 WLVDSKGLIVHSRKESLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEV 429
           WLVDSKGLIV SR ESLQ FKKPWAHEHEPVK LL AVKAIKPTVLIGSSG G+TFTKEV
Sbjct: 368 WLVDSKGLIVSSRLESLQQFKKPWAHEHEPVKGLLEAVKAIKPTVLIGSSGAGKTFTKEV 427

Query: 430 VEAVSSINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEYNGKTFVPGQ 489
           VE ++S+NEKPLI+ALSNPTSQSECTAEEAYTWS+GRAIFASGSPFDP EY GK FVPGQ
Sbjct: 428 VETMASLNEKPLILALSNPTSQSECTAEEAYTWSKGRAIFASGSPFDPVEYEGKLFVPGQ 487

Query: 490 ANNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPPFTNIRKISA 549
           ANNAYIFPGFGLG+++SGAIRV D+MLLAASEALAAQVSEENYDKGLIYPPFTNIRKISA
Sbjct: 488 ANNAYIFPGFGLGLIMSGAIRVRDEMLLAASEALAAQVSEENYDKGLIYPPFTNIRKISA 547

Query: 550 NIAANVAAKAYELGLATRLPRPADLVKYAESCMYSPVYRTYR 570
           NIAA VAAKAY+LGLA+ L RP DLVKYAESCMYSP YR+YR
Sbjct: 548 NIAAKVAAKAYDLGLASHLKRPKDLVKYAESCMYSPGYRSYR 589

[Close]

BLAST of Spg022718 vs. ExPASy Swiss-Prot
Match: P36444 (NADP-dependent malic enzyme, chloroplastic OS=Flaveria pringlei OX=4226 GN=MODA PE=2 SV=1)

HSP 1 Score: 934.5 bits (2414), Expect = 5.7e-271
Identity = 456/573 (79.58%), Postives = 512/573 (89.35%), Query Frame = 0


Query: 19  PKSTVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPHHNKGLAFTEKERDAHYLR 78
           P + VGGGVED+YGED ATE+  +TPW+ SVASGYSLLRDPHHNKGLAFTEKERDAHYLR
Sbjct: 75  PTAVVGGGVEDVYGEDSATEDHFITPWSVSVASGYSLLRDPHHNKGLAFTEKERDAHYLR 134

Query: 79  GLLPPAIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNERLFYKLLIDNVEELLPVV 138
           GLLPP +V   LQ KK+M NIRQY++PLQ++ AMM+LQERNERLFYKLLI+N+EELLP+V
Sbjct: 135 GLLPPVVVNHDLQVKKMMHNIRQYEVPLQRYQAMMDLQERNERLFYKLLIENIEELLPIV 194

Query: 139 YTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQRSIQVIVVTDGERILGLGD 198
           YTPTVGEACQKYG+IF+ PQGLYISLK+KGK+LE+LKNWPQ+ IQVIVVTDGERILGLGD
Sbjct: 195 YTPTVGEACQKYGTIFKNPQGLYISLKDKGKVLEILKNWPQKKIQVIVVTDGERILGLGD 254

Query: 199 LGCQ----------------------CLPITIDVGTNNEKLLNDEFYIGLKQKRATGEEY 258
           LGCQ                      CLPITIDVGTNNEK+LNDEFYIGL+Q+RA+G+EY
Sbjct: 255 LGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEKMLNDEFYIGLRQRRASGKEY 314

Query: 259 YELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASVVLAGA 318
            EL++EFM+AVKQNYGEKVLIQFEDFANHNAF+LL KYRT+HLVFNDDIQGTASVVLAG 
Sbjct: 315 AELMNEFMSAVKQNYGEKVLIQFEDFANHNAFDLLEKYRTTHLVFNDDIQGTASVVLAGL 374

Query: 319 VSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTNAPVEETRKKIWLVDSKGLI 378
           +SALKL+GG+LADH FLFLGAGEAGTGIAELIALE+SKQTNAP+EETRKKIWLVDSKGLI
Sbjct: 375 ISALKLVGGSLADHKFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKIWLVDSKGLI 434

Query: 379 VHSRKESLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAVSSINE 438
           V SR +SLQHFKKPWAH+HEPV   L AVKAIKPTVLIGSSG G+TFTKEVVEA+SS NE
Sbjct: 435 VRSRLDSLQHFKKPWAHDHEPVNKFLDAVKAIKPTVLIGSSGAGQTFTKEVVEAMSSFNE 494

Query: 439 KPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEYNGKTFVPGQANNAYIFPG 498
           KP+I+ALSNPTSQSECTAE+AYTWSEGR IFASGSPF P EYNGK +V GQ+NNAYIFPG
Sbjct: 495 KPIILALSNPTSQSECTAEQAYTWSEGRTIFASGSPFAPVEYNGKVYVSGQSNNAYIFPG 554

Query: 499 FGLGVVISGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPPFTNIRKISANIAANVAAK 558
           FGLG++ISGAIRVHD+MLLAASEALA QV++E++D GLIYPPFTNIRKISA+IAA VAAK
Sbjct: 555 FGLGLIISGAIRVHDEMLLAASEALAEQVTQEHFDNGLIYPPFTNIRKISAHIAAKVAAK 614

Query: 559 AYELGLATRLPRPADLVKYAESCMYSPVYRTYR 570
           AYELGLA+RLP+P +LV YAESCMYSP YR YR
Sbjct: 615 AYELGLASRLPQPENLVAYAESCMYSPKYRNYR 647

[Close]

BLAST of Spg022718 vs. ExPASy Swiss-Prot
Match: O82191 (NADP-dependent malic enzyme 1 OS=Arabidopsis thaliana OX=3702 GN=NADP-ME1 PE=1 SV=1)

HSP 1 Score: 918.7 bits (2373), Expect = 3.3e-266
Identity = 462/578 (79.93%), Postives = 510/578 (88.24%), Query Frame = 0


Query: 14  VLDLDPKSTVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPHHNKGLAFTEKERD 73
           V + D KS+V GGV D+YGED AT E  +TPW+ SV+SGYSLLRDP +NKGLAFTEKERD
Sbjct: 4   VTNSDLKSSVDGGVVDVYGEDSATIEHNITPWSLSVSSGYSLLRDPRYNKGLAFTEKERD 63

Query: 74  AHYLRGLLPPAIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNERLFYKLLIDNVEE 133
            HYLRGLLPP ++ Q+LQEK+L+ NIRQYQ PLQK++A+ ELQERNERLFYKLLIDNVEE
Sbjct: 64  THYLRGLLPPVVLDQKLQEKRLLNNIRQYQFPLQKYMALTELQERNERLFYKLLIDNVEE 123

Query: 134 LLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQRSIQVIVVTDGERI 193
           LLP+VYTPTVGEACQK+GSIFRRPQGL+ISLK+KGKIL+VLKNWP+R+IQVIVVTDGERI
Sbjct: 124 LLPIVYTPTVGEACQKFGSIFRRPQGLFISLKDKGKILDVLKNWPERNIQVIVVTDGERI 183

Query: 194 LGLGDLGCQ----------------------CLPITIDVGTNNEKLLNDEFYIGLKQKRA 253
           LGLGDLGCQ                      CLP+TIDVGTNNEKLLNDEFYIGL+QKRA
Sbjct: 184 LGLGDLGCQGMGIPVGKLALYSALGGVRPSACLPVTIDVGTNNEKLLNDEFYIGLRQKRA 243

Query: 254 TGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASV 313
           TG+EY ELL+EFM+AVKQNYGEKVLIQFEDFANHNAFELLAKY  +HLVFNDDIQGTASV
Sbjct: 244 TGQEYSELLNEFMSAVKQNYGEKVLIQFEDFANHNAFELLAKYSDTHLVFNDDIQGTASV 303

Query: 314 VLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTNAPVEETRKKIWLVD 373
           VLAG VSA KL    LA+HTFLFLGAGEAGTGIAELIAL +SKQ NA VEE+RKKIWLVD
Sbjct: 304 VLAGLVSAQKLTNSPLAEHTFLFLGAGEAGTGIAELIALYMSKQMNASVEESRKKIWLVD 363

Query: 374 SKGLIVHSRKESLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAV 433
           SKGLIV+SRK+SLQ FKKPWAHEHEPVKDLL A+KAIKPTVLIGSSGVGR+FTKEV+EA+
Sbjct: 364 SKGLIVNSRKDSLQDFKKPWAHEHEPVKDLLGAIKAIKPTVLIGSSGVGRSFTKEVIEAM 423

Query: 434 SSINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEYNGKTFVPGQANNA 493
           SSINE+PLIMALSNPT+QSECTAEEAYTWS+GRAIFASGSPFDP EY GK FV  QANNA
Sbjct: 424 SSINERPLIMALSNPTTQSECTAEEAYTWSKGRAIFASGSPFDPVEYEGKVFVSTQANNA 483

Query: 494 YIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPPFTNIRKISANIAA 553
           YIFPGFGLG+VISGAIRVHDDMLLAA+EALA QVS+ENY+KG+IYP F++IRKISA IAA
Sbjct: 484 YIFPGFGLGLVISGAIRVHDDMLLAAAEALAGQVSKENYEKGMIYPSFSSIRKISAQIAA 543

Query: 554 NVAAKAYELGLATRLPRPADLVKYAESCMYSPVYRTYR 570
           NVA KAYELGLA RLPRP D+VK AES MYSP YR YR
Sbjct: 544 NVATKAYELGLAGRLPRPKDIVKCAESSMYSPTYRLYR 581

[Close]

BLAST of Spg022718 vs. ExPASy TrEMBL
Match: A0A0A0LW91 (Malic enzyme OS=Cucumis sativus OX=3659 GN=Csa_1G574870 PE=3 SV=1)

HSP 1 Score: 1103.6 bits (2853), Expect = 0.0e+00
Identity = 560/591 (94.75%), Postives = 567/591 (95.94%), Query Frame = 0


Query: 1   MESTLKEIGDGGSVLDLDPKSTVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH 60
           MESTLKEIGDGGSVLDLDPK+TVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH
Sbjct: 1   MESTLKEIGDGGSVLDLDPKATVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH 60

Query: 61  HNKGLAFTEKERDAHYLRGLLPPAIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120
           HNKGLAFTEKERDAHYLRGLLPPAIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE
Sbjct: 61  HNKGLAFTEKERDAHYLRGLLPPAIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120

Query: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180
           RLFYKLL+DNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR
Sbjct: 121 RLFYKLLVDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180

Query: 181 SIQVIVVTDGERILGLGDLGCQ----------------------CLPITIDVGTNNEKLL 240
           SIQVIVVTDGERILGLGDLGCQ                      CLPITIDVGTNNEKLL
Sbjct: 181 SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240

Query: 241 NDEFYIGLKQKRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSH 300
           NDEFYIGLKQ+RATGEEYYELL EFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRT+H
Sbjct: 241 NDEFYIGLKQRRATGEEYYELLDEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTTH 300

Query: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTNA 360
           LVFNDDIQGTA+VVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEVSKQT A
Sbjct: 301 LVFNDDIQGTAAVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTKA 360

Query: 361 PVEETRKKIWLVDSKGLIVHSRKESLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSG 420
           PVEETRKKIWLVDSKGLIVHSRK+SLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSG
Sbjct: 361 PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSG 420

Query: 421 VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY 480
           VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY
Sbjct: 421 VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY 480

Query: 481 NGKTFVPGQANNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPP 540
           NGKTFVPGQ+NNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPP
Sbjct: 481 NGKTFVPGQSNNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPP 540

Query: 541 FTNIRKISANIAANVAAKAYELGLATRLPRPADLVKYAESCMYSPVYRTYR 570
           FTNIRKISANIAANVAAKAYELGLATRLPRPADLVKYAESCMYSPVYRTYR
Sbjct: 541 FTNIRKISANIAANVAAKAYELGLATRLPRPADLVKYAESCMYSPVYRTYR 591

[Close]

BLAST of Spg022718 vs. ExPASy TrEMBL
Match: A0A5D3CG29 (Malic enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G002990 PE=3 SV=1)

HSP 1 Score: 1101.7 bits (2848), Expect = 0.0e+00
Identity = 558/591 (94.42%), Postives = 567/591 (95.94%), Query Frame = 0


Query: 1   MESTLKEIGDGGSVLDLDPKSTVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH 60
           MESTLKEIGDGGSVLDLDPK+TVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH
Sbjct: 1   MESTLKEIGDGGSVLDLDPKATVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH 60

Query: 61  HNKGLAFTEKERDAHYLRGLLPPAIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120
           HNKGLAFTEKERDAHYLRGLLPPAIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE
Sbjct: 61  HNKGLAFTEKERDAHYLRGLLPPAIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120

Query: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180
           RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR
Sbjct: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180

Query: 181 SIQVIVVTDGERILGLGDLGCQ----------------------CLPITIDVGTNNEKLL 240
           SIQVIVVTDGERILGLGDLGCQ                      CLPITIDVGTNNEKLL
Sbjct: 181 SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240

Query: 241 NDEFYIGLKQKRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSH 300
           NDEFYIGLKQ+RATGEEYYELL EFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRT+H
Sbjct: 241 NDEFYIGLKQRRATGEEYYELLDEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTTH 300

Query: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTNA 360
           LVFNDDIQGTA+VVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALE+SKQT A
Sbjct: 301 LVFNDDIQGTAAVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTKA 360

Query: 361 PVEETRKKIWLVDSKGLIVHSRKESLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSG 420
           PVEETRKKIWLVDSKGLIVHSRK+SLQHFK+PWAHEHEPVKDLLSAVKAIKPTVLIGSSG
Sbjct: 361 PVEETRKKIWLVDSKGLIVHSRKDSLQHFKQPWAHEHEPVKDLLSAVKAIKPTVLIGSSG 420

Query: 421 VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY 480
           VGRTFTKEV+EAVSSINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY
Sbjct: 421 VGRTFTKEVIEAVSSINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY 480

Query: 481 NGKTFVPGQANNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPP 540
           NGKTFVPGQ+NNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPP
Sbjct: 481 NGKTFVPGQSNNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPP 540

Query: 541 FTNIRKISANIAANVAAKAYELGLATRLPRPADLVKYAESCMYSPVYRTYR 570
           FTNIRKISANIAANVAAKAYELGLATRLPRPADLVKYAESCMYSPVYRTYR
Sbjct: 541 FTNIRKISANIAANVAAKAYELGLATRLPRPADLVKYAESCMYSPVYRTYR 591

[Close]

BLAST of Spg022718 vs. ExPASy TrEMBL
Match: A0A1S3BQ08 (Malic enzyme OS=Cucumis melo OX=3656 GN=LOC103492256 PE=3 SV=1)

HSP 1 Score: 1101.7 bits (2848), Expect = 0.0e+00
Identity = 558/591 (94.42%), Postives = 567/591 (95.94%), Query Frame = 0


Query: 1   MESTLKEIGDGGSVLDLDPKSTVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH 60
           MESTLKEIGDGGSVLDLDPK+TVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH
Sbjct: 1   MESTLKEIGDGGSVLDLDPKATVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH 60

Query: 61  HNKGLAFTEKERDAHYLRGLLPPAIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120
           HNKGLAFTEKERDAHYLRGLLPPAIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE
Sbjct: 61  HNKGLAFTEKERDAHYLRGLLPPAIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120

Query: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180
           RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR
Sbjct: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180

Query: 181 SIQVIVVTDGERILGLGDLGCQ----------------------CLPITIDVGTNNEKLL 240
           SIQVIVVTDGERILGLGDLGCQ                      CLPITIDVGTNNEKLL
Sbjct: 181 SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240

Query: 241 NDEFYIGLKQKRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSH 300
           NDEFYIGLKQ+RATGEEYYELL EFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRT+H
Sbjct: 241 NDEFYIGLKQRRATGEEYYELLDEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTTH 300

Query: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTNA 360
           LVFNDDIQGTA+VVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALE+SKQT A
Sbjct: 301 LVFNDDIQGTAAVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTKA 360

Query: 361 PVEETRKKIWLVDSKGLIVHSRKESLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSG 420
           PVEETRKKIWLVDSKGLIVHSRK+SLQHFK+PWAHEHEPVKDLLSAVKAIKPTVLIGSSG
Sbjct: 361 PVEETRKKIWLVDSKGLIVHSRKDSLQHFKQPWAHEHEPVKDLLSAVKAIKPTVLIGSSG 420

Query: 421 VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY 480
           VGRTFTKEV+EAVSSINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY
Sbjct: 421 VGRTFTKEVIEAVSSINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY 480

Query: 481 NGKTFVPGQANNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPP 540
           NGKTFVPGQ+NNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPP
Sbjct: 481 NGKTFVPGQSNNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPP 540

Query: 541 FTNIRKISANIAANVAAKAYELGLATRLPRPADLVKYAESCMYSPVYRTYR 570
           FTNIRKISANIAANVAAKAYELGLATRLPRPADLVKYAESCMYSPVYRTYR
Sbjct: 541 FTNIRKISANIAANVAAKAYELGLATRLPRPADLVKYAESCMYSPVYRTYR 591

[Close]

BLAST of Spg022718 vs. ExPASy TrEMBL
Match: A0A6J1JEY0 (Malic enzyme OS=Cucurbita maxima OX=3661 GN=LOC111485147 PE=3 SV=1)

HSP 1 Score: 1099.0 bits (2841), Expect = 0.0e+00
Identity = 555/591 (93.91%), Postives = 566/591 (95.77%), Query Frame = 0


Query: 1   MESTLKEIGDGGSVLDLDPKSTVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH 60
           MESTLKEIGDGGSVLDLDPK TVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH
Sbjct: 1   MESTLKEIGDGGSVLDLDPKGTVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH 60

Query: 61  HNKGLAFTEKERDAHYLRGLLPPAIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120
           HNKGLAFTEKERDAHYLRGLLPP+IVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE
Sbjct: 61  HNKGLAFTEKERDAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120

Query: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180
           RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR
Sbjct: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180

Query: 181 SIQVIVVTDGERILGLGDLGCQ----------------------CLPITIDVGTNNEKLL 240
           SIQVIVVTDGERILGLGDLGCQ                      CLPITIDVGTNNEKLL
Sbjct: 181 SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240

Query: 241 NDEFYIGLKQKRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSH 300
           NDEFYIGLKQ+RATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRT+H
Sbjct: 241 NDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTTH 300

Query: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTNA 360
           LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEVSKQT A
Sbjct: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTKA 360

Query: 361 PVEETRKKIWLVDSKGLIVHSRKESLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSG 420
           PVEETRKKIWLVDSKGLIVHSRK+SLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSG
Sbjct: 361 PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSG 420

Query: 421 VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY 480
           VGRTFTKEV+EAVS+INEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY
Sbjct: 421 VGRTFTKEVIEAVSAINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY 480

Query: 481 NGKTFVPGQANNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPP 540
           NGKTFVPGQ+NNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAA+VS+ENY+KGLIYPP
Sbjct: 481 NGKTFVPGQSNNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAEVSDENYEKGLIYPP 540

Query: 541 FTNIRKISANIAANVAAKAYELGLATRLPRPADLVKYAESCMYSPVYRTYR 570
           FTNIRKISANIAA VAAKAYELGLATRLPRPADLVKYAESCMYSP+YRTYR
Sbjct: 541 FTNIRKISANIAAKVAAKAYELGLATRLPRPADLVKYAESCMYSPIYRTYR 591

[Close]

BLAST of Spg022718 vs. ExPASy TrEMBL
Match: A0A6J1H992 (Malic enzyme OS=Cucurbita moschata OX=3662 GN=LOC111461666 PE=3 SV=1)

HSP 1 Score: 1099.0 bits (2841), Expect = 0.0e+00
Identity = 554/591 (93.74%), Postives = 566/591 (95.77%), Query Frame = 0


Query: 1   MESTLKEIGDGGSVLDLDPKSTVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH 60
           MESTLKEIGDGGSVLDLDPK TVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH
Sbjct: 1   MESTLKEIGDGGSVLDLDPKGTVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPH 60

Query: 61  HNKGLAFTEKERDAHYLRGLLPPAIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120
           HNKGLAFTEKERDAHYLRGLLPP+IVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE
Sbjct: 61  HNKGLAFTEKERDAHYLRGLLPPSIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNE 120

Query: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180
           RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR
Sbjct: 121 RLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQR 180

Query: 181 SIQVIVVTDGERILGLGDLGCQ----------------------CLPITIDVGTNNEKLL 240
           SIQVIVVTDGERILGLGDLGCQ                      CLPITIDVGTNNEKLL
Sbjct: 181 SIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLL 240

Query: 241 NDEFYIGLKQKRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSH 300
           NDEFYIGLKQ+RATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRT+H
Sbjct: 241 NDEFYIGLKQRRATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTTH 300

Query: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTNA 360
           LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALE+SKQTNA
Sbjct: 301 LVFNDDIQGTASVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTNA 360

Query: 361 PVEETRKKIWLVDSKGLIVHSRKESLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSG 420
           PVEETRKKIWLVDSKGLIVHSRK+SLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSG
Sbjct: 361 PVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSG 420

Query: 421 VGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY 480
           VGRTFTKEV+EAVS+INEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY
Sbjct: 421 VGRTFTKEVIEAVSAINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY 480

Query: 481 NGKTFVPGQANNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPP 540
           NGKTFVPGQ+NNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAA+VS+ENY+ GLIYPP
Sbjct: 481 NGKTFVPGQSNNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAEVSDENYENGLIYPP 540

Query: 541 FTNIRKISANIAANVAAKAYELGLATRLPRPADLVKYAESCMYSPVYRTYR 570
           FTNIRKISANIAA VAAKAYELGLATRLPRPADLVKYAESCMYSP+YRTYR
Sbjct: 541 FTNIRKISANIAAKVAAKAYELGLATRLPRPADLVKYAESCMYSPIYRTYR 591

[Close]

BLAST of Spg022718 vs. TAIR 10
Match: AT2G19900.1 (NADP-malic enzyme 1 )

HSP 1 Score: 918.7 bits (2373), Expect = 2.3e-267
Identity = 462/578 (79.93%), Postives = 510/578 (88.24%), Query Frame = 0


Query: 14  VLDLDPKSTVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPHHNKGLAFTEKERD 73
           V + D KS+V GGV D+YGED AT E  +TPW+ SV+SGYSLLRDP +NKGLAFTEKERD
Sbjct: 4   VTNSDLKSSVDGGVVDVYGEDSATIEHNITPWSLSVSSGYSLLRDPRYNKGLAFTEKERD 63

Query: 74  AHYLRGLLPPAIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNERLFYKLLIDNVEE 133
            HYLRGLLPP ++ Q+LQEK+L+ NIRQYQ PLQK++A+ ELQERNERLFYKLLIDNVEE
Sbjct: 64  THYLRGLLPPVVLDQKLQEKRLLNNIRQYQFPLQKYMALTELQERNERLFYKLLIDNVEE 123

Query: 134 LLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQRSIQVIVVTDGERI 193
           LLP+VYTPTVGEACQK+GSIFRRPQGL+ISLK+KGKIL+VLKNWP+R+IQVIVVTDGERI
Sbjct: 124 LLPIVYTPTVGEACQKFGSIFRRPQGLFISLKDKGKILDVLKNWPERNIQVIVVTDGERI 183

Query: 194 LGLGDLGCQ----------------------CLPITIDVGTNNEKLLNDEFYIGLKQKRA 253
           LGLGDLGCQ                      CLP+TIDVGTNNEKLLNDEFYIGL+QKRA
Sbjct: 184 LGLGDLGCQGMGIPVGKLALYSALGGVRPSACLPVTIDVGTNNEKLLNDEFYIGLRQKRA 243

Query: 254 TGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASV 313
           TG+EY ELL+EFM+AVKQNYGEKVLIQFEDFANHNAFELLAKY  +HLVFNDDIQGTASV
Sbjct: 244 TGQEYSELLNEFMSAVKQNYGEKVLIQFEDFANHNAFELLAKYSDTHLVFNDDIQGTASV 303

Query: 314 VLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTNAPVEETRKKIWLVD 373
           VLAG VSA KL    LA+HTFLFLGAGEAGTGIAELIAL +SKQ NA VEE+RKKIWLVD
Sbjct: 304 VLAGLVSAQKLTNSPLAEHTFLFLGAGEAGTGIAELIALYMSKQMNASVEESRKKIWLVD 363

Query: 374 SKGLIVHSRKESLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAV 433
           SKGLIV+SRK+SLQ FKKPWAHEHEPVKDLL A+KAIKPTVLIGSSGVGR+FTKEV+EA+
Sbjct: 364 SKGLIVNSRKDSLQDFKKPWAHEHEPVKDLLGAIKAIKPTVLIGSSGVGRSFTKEVIEAM 423

Query: 434 SSINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEYNGKTFVPGQANNA 493
           SSINE+PLIMALSNPT+QSECTAEEAYTWS+GRAIFASGSPFDP EY GK FV  QANNA
Sbjct: 424 SSINERPLIMALSNPTTQSECTAEEAYTWSKGRAIFASGSPFDPVEYEGKVFVSTQANNA 483

Query: 494 YIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPPFTNIRKISANIAA 553
           YIFPGFGLG+VISGAIRVHDDMLLAA+EALA QVS+ENY+KG+IYP F++IRKISA IAA
Sbjct: 484 YIFPGFGLGLVISGAIRVHDDMLLAAAEALAGQVSKENYEKGMIYPSFSSIRKISAQIAA 543

Query: 554 NVAAKAYELGLATRLPRPADLVKYAESCMYSPVYRTYR 570
           NVA KAYELGLA RLPRP D+VK AES MYSP YR YR
Sbjct: 544 NVATKAYELGLAGRLPRPKDIVKCAESSMYSPTYRLYR 581

[Close]

BLAST of Spg022718 vs. TAIR 10
Match: AT1G79750.1 (NADP-malic enzyme 4 )

HSP 1 Score: 914.1 bits (2361), Expect = 5.7e-266
Identity = 442/579 (76.34%), Postives = 514/579 (88.77%), Query Frame = 0


Query: 13  SVLDLDPKSTVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPHHNKGLAFTEKER 72
           S  D+ PKSTV GGV+D+YGED ATE+  +TPW+ SVASGY+LLRDPHHNKGLAF+ +ER
Sbjct: 68  SAADIVPKSTVSGGVQDVYGEDAATEDMPITPWSLSVASGYTLLRDPHHNKGLAFSHRER 127

Query: 73  DAHYLRGLLPPAIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNERLFYKLLIDNVE 132
           DAHYLRGLLPP +++Q LQ KK+M  +RQYQ+PLQK++AMM+LQE NERLFYKLLID+VE
Sbjct: 128 DAHYLRGLLPPTVISQDLQVKKIMHTLRQYQVPLQKYMAMMDLQETNERLFYKLLIDHVE 187

Query: 133 ELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQRSIQVIVVTDGER 192
           ELLPV+YTPTVGEACQKYGSIF RPQGL+ISLKEKGKI EVL+NWP+++IQVIVVTDGER
Sbjct: 188 ELLPVIYTPTVGEACQKYGSIFLRPQGLFISLKEKGKIHEVLRNWPEKNIQVIVVTDGER 247

Query: 193 ILGLGDLGCQ----------------------CLPITIDVGTNNEKLLNDEFYIGLKQKR 252
           ILGLGDLGCQ                      CLP+TIDVGTNNEKLLNDEFYIGL+Q+R
Sbjct: 248 ILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEKLLNDEFYIGLRQRR 307

Query: 253 ATGEEYYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTAS 312
           ATGEEY EL+HEFMTAVKQNYGEKV+IQFEDFANHNAF+LLAKY T+HLVFNDDIQGTAS
Sbjct: 308 ATGEEYSELMHEFMTAVKQNYGEKVVIQFEDFANHNAFDLLAKYGTTHLVFNDDIQGTAS 367

Query: 313 VVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTNAPVEETRKKIWLV 372
           VVLAG ++AL+ +GG+L+DH FLFLGAGEAGTGIAELIALE+SK+++ P+EE RK IWLV
Sbjct: 368 VVLAGLIAALRFVGGSLSDHRFLFLGAGEAGTGIAELIALEISKKSHIPLEEARKNIWLV 427

Query: 373 DSKGLIVHSRKESLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEA 432
           DSKGLIV SRKES+QHFKKPWAH+HEP+++L+ AVKAIKPTVLIG+SGVG+TFT++VVE 
Sbjct: 428 DSKGLIVSSRKESIQHFKKPWAHDHEPIRELVDAVKAIKPTVLIGTSGVGQTFTQDVVET 487

Query: 433 VSSINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEYNGKTFVPGQANN 492
           ++ +NEKP+I++LSNPTSQSECTAEEAYTWS+GRAIFASGSPF P EY GKTFVPGQANN
Sbjct: 488 MAKLNEKPIILSLSNPTSQSECTAEEAYTWSQGRAIFASGSPFAPVEYEGKTFVPGQANN 547

Query: 493 AYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPPFTNIRKISANIA 552
           AYIFPGFGLG+++SG IRVHDDMLLAASEALA ++ EE+Y+KG+IYPPF NIRKISA IA
Sbjct: 548 AYIFPGFGLGLIMSGTIRVHDDMLLAASEALAEELMEEHYEKGMIYPPFRNIRKISARIA 607

Query: 553 ANVAAKAYELGLATRLPRPADLVKYAESCMYSPVYRTYR 570
           A VAAKAYELGLATRLP+P +L + AES MYSP YR+YR
Sbjct: 608 AKVAAKAYELGLATRLPQPKELEQCAESSMYSPSYRSYR 646

[Close]

BLAST of Spg022718 vs. TAIR 10
Match: AT5G25880.1 (NADP-malic enzyme 3 )

HSP 1 Score: 907.1 bits (2343), Expect = 7.0e-264
Identity = 444/571 (77.76%), Postives = 507/571 (88.79%), Query Frame = 0


Query: 21  STVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPHHNKGLAFTEKERDAHYLRGL 80
           S VGGG+ D+YGED AT +QLVTPW  SVASGY+L+RDP +NKGLAFT+KERDAHY+ GL
Sbjct: 18  SGVGGGISDVYGEDSATLDQLVTPWVTSVASGYTLMRDPRYNKGLAFTDKERDAHYITGL 77

Query: 81  LPPAIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNERLFYKLLIDNVEELLPVVYT 140
           LPP +++Q +QE+K+M N+RQY +PLQ+++A+M+LQERNERLFYKLLIDNVEELLPVVYT
Sbjct: 78  LPPVVLSQDVQERKVMHNLRQYTVPLQRYMALMDLQERNERLFYKLLIDNVEELLPVVYT 137

Query: 141 PTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQRSIQVIVVTDGERILGLGDLG 200
           PTVGEACQKYGSI+RRPQGLYISLKEKGKILEVLKNWPQR IQVIVVTDGERILGLGDLG
Sbjct: 138 PTVGEACQKYGSIYRRPQGLYISLKEKGKILEVLKNWPQRGIQVIVVTDGERILGLGDLG 197

Query: 201 CQ----------------------CLPITIDVGTNNEKLLNDEFYIGLKQKRATGEEYYE 260
           CQ                      CLPITIDVGTNNEKLLN+EFYIGLKQKRA GEEY E
Sbjct: 198 CQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEKLLNNEFYIGLKQKRANGEEYAE 257

Query: 261 LLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASVVLAGAVS 320
            L EFM AVKQNYGEKVL+QFEDFANH+AFELL+KY +SHLVFNDDIQGTASVVLAG ++
Sbjct: 258 FLQEFMCAVKQNYGEKVLVQFEDFANHHAFELLSKYCSSHLVFNDDIQGTASVVLAGLIA 317

Query: 321 ALKLIGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTNAPVEETRKKIWLVDSKGLIVH 380
           A K++G +LADHTFLFLGAGEAGTGIAELIAL++SK+T  P++ETRKKIWLVDSKGLIV 
Sbjct: 318 AQKVLGKSLADHTFLFLGAGEAGTGIAELIALKISKETGKPIDETRKKIWLVDSKGLIVS 377

Query: 381 SRKESLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAVSSINEKP 440
            RKESLQHFK+PWAH+H+PVK+LL+AV AIKPTVLIG+SGVG+TFTKEVVEA++++NEKP
Sbjct: 378 ERKESLQHFKQPWAHDHKPVKELLAAVNAIKPTVLIGTSGVGKTFTKEVVEAMATLNEKP 437

Query: 441 LIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEYNGKTFVPGQANNAYIFPGFG 500
           LI+ALSNPTSQ+ECTAEEAYTW++GRAIFASGSPFDP +Y+GK F PGQANN YIFPG G
Sbjct: 438 LILALSNPTSQAECTAEEAYTWTKGRAIFASGSPFDPVQYDGKKFTPGQANNCYIFPGLG 497

Query: 501 LGVVISGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPPFTNIRKISANIAANVAAKAY 560
           LG+++SGAIRV DDMLLAASEALA+QV+EEN+  GLIYPPF NIRKISANIAA+V AK Y
Sbjct: 498 LGLIMSGAIRVRDDMLLAASEALASQVTEENFANGLIYPPFANIRKISANIAASVGAKTY 557

Query: 561 ELGLATRLPRPADLVKYAESCMYSPVYRTYR 570
           ELGLA+ LPRP DLVK AESCMYSPVYR +R
Sbjct: 558 ELGLASNLPRPKDLVKMAESCMYSPVYRNFR 588

[Close]

BLAST of Spg022718 vs. TAIR 10
Match: AT5G11670.1 (NADP-malic enzyme 2 )

HSP 1 Score: 906.7 bits (2342), Expect = 9.1e-264
Identity = 447/574 (77.87%), Postives = 507/574 (88.33%), Query Frame = 0


Query: 18  DPKSTVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPHHNKGLAFTEKERDAHYL 77
           D +S VGGG+ D+YGED AT +QLVTPW  SVASGY+L+RDP +NKGLAFT+KERDAHYL
Sbjct: 15  DNRSGVGGGISDVYGEDSATLDQLVTPWVTSVASGYTLMRDPRYNKGLAFTDKERDAHYL 74

Query: 78  RGLLPPAIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNERLFYKLLIDNVEELLPV 137
            GLLPP I++Q +QE+K+M N+RQY +PLQ+++A+M+LQERNERLFYKLLIDNVEELLPV
Sbjct: 75  TGLLPPVILSQDVQERKVMHNLRQYTVPLQRYMALMDLQERNERLFYKLLIDNVEELLPV 134

Query: 138 VYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQRSIQVIVVTDGERILGLG 197
           VYTPTVGEACQKYGSIFR+PQGLYISL EKGKILEVLKNWPQR IQVIVVTDGERILGLG
Sbjct: 135 VYTPTVGEACQKYGSIFRKPQGLYISLNEKGKILEVLKNWPQRGIQVIVVTDGERILGLG 194

Query: 198 DLGCQ----------------------CLPITIDVGTNNEKLLNDEFYIGLKQKRATGEE 257
           DLGCQ                      CLPITIDVGTNNEKLLNDEFYIGLKQ+RATG+E
Sbjct: 195 DLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEKLLNDEFYIGLKQRRATGQE 254

Query: 258 YYELLHEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTSHLVFNDDIQGTASVVLAG 317
           Y E LHEFM AVKQNYGEKVL+QFEDFANHNAF+LL+KY  SHLVFNDDIQGTASVVLAG
Sbjct: 255 YAEFLHEFMCAVKQNYGEKVLVQFEDFANHNAFDLLSKYSDSHLVFNDDIQGTASVVLAG 314

Query: 318 AVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTNAPVEETRKKIWLVDSKGL 377
            ++A K++G  LADHTFLFLGAGEAGTGIAELIAL++SK+T AP+ ETRKKIWLVDSKGL
Sbjct: 315 LIAAQKVLGKKLADHTFLFLGAGEAGTGIAELIALKISKETGAPITETRKKIWLVDSKGL 374

Query: 378 IVHSRKESLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAVSSIN 437
           IV SRKESLQHFK+PWAHEH+PVKDL+ AV AIKPTVLIG+SGVG+TFTKEVVEA+++ N
Sbjct: 375 IVSSRKESLQHFKQPWAHEHKPVKDLIGAVNAIKPTVLIGTSGVGQTFTKEVVEAMATNN 434

Query: 438 EKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEYNGKTFVPGQANNAYIFP 497
           EKPLI+ALSNPTSQ+ECTAE+AYTW++GRAIF SGSPFDP  Y+GKT++PGQANN YIFP
Sbjct: 435 EKPLILALSNPTSQAECTAEQAYTWTKGRAIFGSGSPFDPVVYDGKTYLPGQANNCYIFP 494

Query: 498 GFGLGVVISGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPPFTNIRKISANIAANVAA 557
           G GLG+++SGAIRV DDMLLAASEALAAQV+EE+Y  GLIYPPF+NIR+ISANIAA VAA
Sbjct: 495 GLGLGLIMSGAIRVRDDMLLAASEALAAQVTEEHYANGLIYPPFSNIREISANIAACVAA 554

Query: 558 KAYELGLATRLPRPADLVKYAESCMYSPVYRTYR 570
           K Y+LGLA+ LPR  DLVK+AES MYSPVYR YR
Sbjct: 555 KTYDLGLASNLPRAKDLVKFAESSMYSPVYRNYR 588

[Close]

BLAST of Spg022718 vs. TAIR 10
Match: AT2G13560.1 (NAD-dependent malic enzyme 1 )

HSP 1 Score: 391.7 bits (1005), Expect = 9.8e-109
Identity = 231/574 (40.24%), Postives = 338/574 (58.89%), Query Frame = 0


Query: 52  GYSLLRDPHHNKGLAFTEKERDAHYLRGLLPPAIVTQQLQEKKLMQNIRQYQ-------- 111
           G  +L DP  NKG AFT  ER+   LRGLLPP ++  + Q  + M ++++ +        
Sbjct: 46  GLDILHDPWFNKGTAFTMTERNRLDLRGLLPPNVMDSEQQIFRFMTDLKRLEEQARDGPS 105

Query: 112 --LPLQKFIAMMELQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLY 171
               L K+  +  L +RNE ++YK+LI+N+EE  P+VYTPTVG  CQ Y  +FRRP+G+Y
Sbjct: 106 DPNALAKWRILNRLHDRNETMYYKVLINNIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMY 165

Query: 172 ISLKEKGKILEVLKNWPQRSIQVIVVTDGERILGLGDLGC-------------------- 231
            S +++G+++ ++ NWP   + +IVVTDG RILGLGDLG                     
Sbjct: 166 FSAEDRGEMMSMVYNWPAEQVDMIVVTDGSRILGLGDLGVHGIGIAVGKLDLYVAAAGIN 225

Query: 232 --QCLPITIDVGTNNEKLLNDEFYIGLKQKRATGEEYYELLHEFMTAVKQNYGEKVLIQF 291
             + LP+ IDVGTNNEKL ND  Y+GL+Q+R   ++Y +++ EFM AV   +   V++QF
Sbjct: 226 PQRVLPVMIDVGTNNEKLRNDPMYLGLQQRRLEDDDYIDVIDEFMEAVYTRW-PHVIVQF 285

Query: 292 EDFANHNAFELLAKYRTSHLVFNDDIQGTASVVLAGAVSALKLIGGTLADH---TFLFLG 351
           EDF +  AF+LL +YR ++ +FNDD+QGTA V +AG + A++  G  + D      +  G
Sbjct: 286 EDFQSKWAFKLLQRYRCTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKMKIVVAG 345

Query: 352 AGEAGTGIAELIALEVSK---QTNAPVEETRKKIWLVDSKGLIVHSRKESLQHFKKPWAH 411
           AG AG G+       +++    T    +  + + W+VD++GLI   R E++    +P+A 
Sbjct: 346 AGSAGIGVLNAARKTMARMLGNTETAFDSAQSQFWVVDAQGLITEGR-ENIDPEAQPFAR 405

Query: 412 -----EHEPVKD---LLSAVKAIKPTVLIGSSGVGRTFTKEVVEAV-SSINEKPLIMALS 471
                E + +K+   L+  V+ +KP VL+G S VG  F+KEV+EA+  S + +P I A+S
Sbjct: 406 KTKEMERQGLKEGATLVEVVREVKPDVLLGLSAVGGLFSKEVLEAMKGSTSTRPAIFAMS 465

Query: 472 NPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEY-NGKTFVPGQANNAYIFPGFGLGVVI 531
           NPT  +ECT ++A++      IFASGSPF   E+ NG      Q NN Y+FPG GLG ++
Sbjct: 466 NPTKNAECTPQDAFSILGENMIFASGSPFKNVEFGNGHVGHCNQGNNMYLFPGIGLGTLL 525

Query: 532 SGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPPFTNIRKISANIAANVAAKAYELGLA 568
           SGA  V D ML AASE LAA +SEE   +G+IYPP + IR I+  IAA V  +A E  L 
Sbjct: 526 SGAPIVSDGMLQAASECLAAYMSEEEVLEGIIYPPISRIRDITKRIAAAVIKEAIEEDLV 585

The following BLAST results are available for this feature:

BLAST of Spg022718 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004135668.1	0.0e+00	94.75	NADP-dependent malic enzyme [Cucumis sativus] >KGN66170.1 hypothetical protein C...	[more]
XP_008450776.1	0.0e+00	94.42	PREDICTED: NADP-dependent malic enzyme [Cucumis melo] >TYK10154.1 NADP-dependent...	[more]
KAG6589949.1	0.0e+00	93.91	hypothetical protein SDJN03_15372, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023516304.1	0.0e+00	93.74	NADP-dependent malic enzyme [Cucurbita pepo subsp. pepo]	[more]
XP_022961041.1	0.0e+00	93.74	NADP-dependent malic enzyme [Cucurbita moschata]	[more]

BLAST of Spg022718 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P51615	3.3e-287	83.59	NADP-dependent malic enzyme OS=Vitis vinifera OX=29760 PE=2 SV=1	[more]
P34105	7.0e-285	82.23	NADP-dependent malic enzyme OS=Populus trichocarpa OX=3694 PE=2 SV=3	[more]
P12628	4.0e-272	80.41	NADP-dependent malic enzyme OS=Phaseolus vulgaris OX=3885 GN=ME1 PE=2 SV=1	[more]
P36444	5.7e-271	79.58	NADP-dependent malic enzyme, chloroplastic OS=Flaveria pringlei OX=4226 GN=MODA ...	[more]
O82191	3.3e-266	79.93	NADP-dependent malic enzyme 1 OS=Arabidopsis thaliana OX=3702 GN=NADP-ME1 PE=1 S...	[more]

BLAST of Spg022718 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LW91	0.0e+00	94.75	Malic enzyme OS=Cucumis sativus OX=3659 GN=Csa_1G574870 PE=3 SV=1	[more]
A0A5D3CG29	0.0e+00	94.42	Malic enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G002990 P...	[more]
A0A1S3BQ08	0.0e+00	94.42	Malic enzyme OS=Cucumis melo OX=3656 GN=LOC103492256 PE=3 SV=1	[more]
A0A6J1JEY0	0.0e+00	93.91	Malic enzyme OS=Cucurbita maxima OX=3661 GN=LOC111485147 PE=3 SV=1	[more]
A0A6J1H992	0.0e+00	93.74	Malic enzyme OS=Cucurbita moschata OX=3662 GN=LOC111461666 PE=3 SV=1	[more]

BLAST of Spg022718 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G19900.1	2.3e-267	79.93	NADP-malic enzyme 1	[more]
AT1G79750.1	5.7e-266	76.34	NADP-malic enzyme 4	[more]
AT5G25880.1	7.0e-264	77.76	NADP-malic enzyme 3	[more]
AT5G11670.1	9.1e-264	77.87	NADP-malic enzyme 2	[more]
AT2G13560.1	9.8e-109	40.24	NAD-dependent malic enzyme 1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001891	Malic oxidoreductase	PRINTS	PR00072	MALOXRDTASE	coord: 122..146 score: 69.8 coord: 182..211 score: 61.25 coord: 415..431 score: 66.62
IPR001891	Malic oxidoreductase	PIRSF	PIRSF000106	ME	coord: 201..569 e-value: 1.4E-153 score: 510.5 coord: 29..203 e-value: 1.4E-60 score: 203.4
IPR012302	Malic enzyme, NAD-binding	SMART	SM00919	Malic_M_2	coord: 285..538 e-value: 3.1E-112 score: 388.9
IPR012302	Malic enzyme, NAD-binding	PFAM	PF03949	Malic_M	coord: 285..537 e-value: 4.2E-95 score: 317.9
IPR012301	Malic enzyme, N-terminal domain	SMART	SM01274	malic_2	coord: 116..275 e-value: 3.7E-78 score: 275.6
IPR012301	Malic enzyme, N-terminal domain	PFAM	PF00390	malic	coord: 116..202 e-value: 1.8E-33 score: 115.9
IPR037062	Malic enzyme, N-terminal domain superfamily	GENE3D	3.40.50.10380		coord: 203..282 e-value: 8.0E-36 score: 125.5 coord: 50..202 e-value: 2.0E-68 score: 232.3
None	No IPR available	GENE3D	3.40.50.720		coord: 283..569 e-value: 4.0E-120 score: 402.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..20
None	No IPR available	PANTHER	PTHR23406	MALIC ENZYME-RELATED	coord: 203..569
None	No IPR available	PANTHER	PTHR23406:SF65	MALIC ENZYME	coord: 203..569
None	No IPR available	PANTHER	PTHR23406:SF65	MALIC ENZYME	coord: 20..202
None	No IPR available	PANTHER	PTHR23406	MALIC ENZYME-RELATED	coord: 20..202
None	No IPR available	CDD	cd05312	NAD_bind_1_malic_enz	coord: 285..563 e-value: 1.3228E-145 score: 419.261
None	No IPR available	SUPERFAMILY	53223	Aminoacid dehydrogenase-like, N-terminal domain	coord: 31..284
IPR015884	Malic enzyme, conserved site	PROSITE	PS00331	MALIC_ENZYMES	coord: 281..297
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 285..568

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg022718.1	Spg022718.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006108	malate metabolic process
biological_process	GO:0006090	pyruvate metabolic process
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0004471	malate dehydrogenase (decarboxylating) (NAD+) activity
molecular_function	GO:0004473	malate dehydrogenase (decarboxylating) (NADP+) activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0051287	NAD binding
molecular_function	GO:0004470	malic enzyme activity