Spg022645 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg022645
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Exonuclease domain-containing protein
Location	scaffold2: 4793793 .. 4797705 (-)
RNA-Seq Expression	Spg022645
Synteny	Spg022645

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGCCGAGTGATCCGTACGTTGGAAGTTAACATCACGAAGCAACCTCTCAAGCCACGGTGCTCGTTATCGTTATAATACCTTCATTTCTAGGCGCCTTCTTGGCTTCTTCTTCTTCAATCTCAACACAAATCTTCTTCCTTTTCTCTCTCCACCCCTCTGCGCTCTTCTCTGATCGAAATTTCCCTCGCTTCAATCATGGGTCCGACGGAGGATCGATCGGAGATTGCCTTCTTTGATGTGGAGACCACTGTTCCCACCCGCCAAGGCCAGAGATTTTCAATTTTGGAGTTTGGCGCGATTCTGGTGTGCCCTAGAAAGCTGGTTGAGTTGGAGAGCTATTCCACTCTCGTTCGCCCCTCTGATCTCTCTCTCATTTCCTCGTTGTCGGTGCGATGCAATGGCATTACTAGAGACGCCGTCATTTCTTCCCCTACTTTCGCCCAAATTGCCGATAGGGTTTACGATATCCTTCACGGTACTCTCTCTCTTTTGCGATCATTCTTATTCTTTACTTGTGTAGTTGAAAGGAAGCGGCGAAATGTTTATTTTGATGAATTGTCCGAGTGGGTTGATAGTTAATTTGATGTAAAAGTTGAATTGGCTCTGAATTTTGAACTTTCAGTCAGTATATTTTTTTGGCAATCAGGTTAATTTAGCTGAACAAAGAGAGTGAAGCTTTTTAAGGTTCGAAGAGTATGTATTTTCTTCGATCATAAACGAAGCCGAGGTTTATTTTCGAAGTCCGTAGATGATATTTTTGAAATGCAGTCGTTCTTCGTTCCTGTTCCTTTTTATTTTTCAGTCAAAAGAACTACATTGTCTGTTCAAGTTTGAATTGATGTTACATATCTGGGAACGATGATCATTACGAAAGATTGTACCATACTCTGTCGGGTTGAATGGTCCATGCTTCTTCTATTTTTATTTTATTATATGCCTGCGTTTGAGTTTCCTACTGGGATTTTGTTTTGTAGGACGGATATGGGCAGGCCATAACATACTGAGATTTGATTGTGCACGAATAAGGGAGGCTTTTGCAGAAATTGGGATGCCAGCTCCTCAACCGAAAGGTACTATCGATTCATTGGCATTGTTGACTCAAAGGTTTGGAAGGAGAGCTGGCGACATGAAGGTAATGACAGAACTCTTTCTCTGTTTCATGCTTGCTATGTTTTAAGTTTTGGGTGATCCCAAGAAACCTGTAAACTAGTATCTATTGGATGGTGCCTTCGGAAAAATTATTAAGAGCTTTCAACCTTCAGAACTCAAGCAGGAAATTGTGAAAATGATTTAAGTTTTGGGATTTACCTCTCTAAATATTCTAAATATGAGACTTAAACTTCGTTATCATTTGGCACTAAAATAAGGGTACATTATGACTGTAACGTCGAGCCAATTATCACTGTTAAGGTTTATGCCAAAGAGAGGCTGGAAAAACCTCCCCCATAGGAGGAGAACTTGAATTCAAACTCCTTCATAGTTATCCGTGCAGAGTTTTCTACTTGTATCCATGGTACAGTGCTCTAAATCTGAATGGAACTTGATTCTGTTACATCCATGACCTTGACCTTGACCCTTTTCAAGAAGTGACCCTATATTTGTGGGTATCTCTTCACTGTCTTCCTTAACTTCTGTGTTTATAGGCTTCTTGTAACGATACTTCCCATCAGATCCTTGCATTTGGCTTCTCTAGTTAATTCAATATTACTGTCTTGGCTTGATATAATGGATCTTCATGCATGTTTCTAATTTTGGGTTTTGATTGTAAAAGTTTATGGCAACTCTTTATTTATTTGATTTTGACGTTCCTTTTCTCCCTGTAGATGGCTACTCTTGCTACCTATTTCGGAATAGGACAACAAACACACAGGCAACTCTTCGAACTTGCTAGCAATTTTTGAACTTAAATCAAAGGAGTGGTTTCGATTTATCTGACTTTGTATGTATAATTCTCCAGGAGTTTGGATGATGTCAGGATGAATCTCGAAGTTCTAAAGTATTGTGCAACTGTCTTGTTTCTGGTAATGTATAAATTTAAGATGTCGAACATAACTAATATTTTTATGTGAACTTGTAACTGTGTCAACGATTCATTGATTGTTTGCAGTTTAGATCATTTTATAATGTTTCTACATGTATGAATTTTTTTTTCATTATCTGAATGTTGACCTCTTGGATGTGTGCCCGTGATAACCCTGATTTGGATCATCATTAGGTTCTAAATGCTTCATCGTTAGTTTTTGCATCAATTGACAATACTTATCTCCATTTCACCTCTGCCATTTGAAAACATTCTTTTAGTAGTTTTTTCTTTCGTTTTTTTTGTTTAAAGTTAACTTAATCTGATATCAGCATAGTGGCATTAGCTTTGCTCGTACGTTGACCATTTTAATTTAGAAAAGTTATGGTGGGTTAGAGTTGAACTGTTGTCTGGCTACAGGAATCTAGTCTACCCGAAGTTTTCCCGGAGAATAGTTGGGTTTCTCCAGATGCTGTTACAAGGCGTCGTGCTGGAAAATCATCTCCACAAGGAGTTAATTCAAATAATAGTACTCACTCATCAAGTTCAAGGATAGGTGGTAATCCAATATCTCTACCAGATCAACAAGGGGAAGCTCATCCGATATTATCTCTTGTAACTTGCAGCTCTGAGGACGGAACCTCAAATCTAGCTGAATCTGATGCTACTGAATCAGATTCTTTTAACATTCACACTCTCAGTGACCAAATAACAGGTGAATCACTAGAAACAGATATCAGCATGGAAGAAGAAGATGTCTCTGAATCTACTGAAGCATCTCCCACAGAGGATACTTCTACAAGTCTCAGCGCTGCTACTGAATTCTTGGAGCCTGATCAGGTGTCTGTTTCGTCCATCACTGCATCTTTCATTCCATTTCTTCGTGGGAGCCAAAGAATACAATTATCGCACAAAGACGACTGTTTACAGCTTCTTTGTAACAATCTGAAAGTTCGGTTTGGCATCAGCACAAAATTCACTGATTATGCTGGCCGTCCAAGGTTGAGTTTCGTGGTCGATGTACCACCAAGTTTATGCAGTGTTCTTGAAGCATCTGATAGCGTGGCTCAGAGATTATTCTCACAGTCTGGCAGCGGCTCCGAGTGGAGACCTGTCGTGGTGAGACCTGTCGTGGTGAGAAAGAATGGTTATTTCAACTACCCAACAATGAGATTACAGTAAGTCATACTCCCATCTGATCATTTGACATGTAAACTGACTACTATTTACAAAATTCTGATGAAAAATTTGAACTATCAAATGAGGGAAAAGGAACAAAAAAGAACAAAGAAAAACTAGTCCAACATATGATCTGCATGCTTGCTTGCTTCAAATTTTTTTATTTCTTGCCAACCAAATCCATTCATCTGCTGTATAAGTCCATAAATTTCCTCTTCTTGGTCAGTTTCTAATGGATGTTAAATCTGTTTTTTCACAGCATTCCAACTGCAGTGAGTGGAGATACGGCCATTTATGCCACAGAGATGTACCAAAAAGAATCATCAGGTGCAGCACAAAGGCTTATATTTAGTAAATTTGATGCTGCAGAGCTTGATAGCTTGATCAACCCTGGAGCCATCTTAGATGCATTCATTTCCTTGGATACATATGATTATCAACAGAGTGCAGGCATTAGATTGGTAGCAAAAAAGTTGATTATCCATTCCTAGTAATGGTAAATCAAGTTGTCCCATTATTGAAGTAGCTCTGTTGTTATATTATGCTTAGTTCACTGACTAACATTCAAATCTCCAATAAATCCCTTTTCGTTATTTTAAGAAAAATCATCACTTTTCCCCATGTCTGCATTGTTTGATTGGAAAAGTACTTTTCTCTTTAATTATTTTAGTATTGTAGTTACACATGTTTAATAAATTTGGGTTTTTGAAG

mRNA sequence

ATGAGCCGAGTGATCCGTACGTTGGAAGCGCCTTCTTGGCTTCTTCTTCTTCAATCTCAACACAAATCTTCTTCCTTTTCTCTCTCCACCCCTCTGCGCTCTTCTCTGATCGAAATTTCCCTCGCTTCAATCATGGGTCCGACGGAGGATCGATCGGAGATTGCCTTCTTTGATGTGGAGACCACTGTTCCCACCCGCCAAGGCCAGAGATTTTCAATTTTGGAGTTTGGCGCGATTCTGGTGTGCCCTAGAAAGCTGGTTGAGTTGGAGAGCTATTCCACTCTCGTTCGCCCCTCTGATCTCTCTCTCATTTCCTCGTTGTCGGTGCGATGCAATGGCATTACTAGAGACGCCGTCATTTCTTCCCCTACTTTCGCCCAAATTGCCGATAGGGTTTACGATATCCTTCACGGACGGATATGGGCAGGCCATAACATACTGAGATTTGATTGTGCACGAATAAGGGAGGCTTTTGCAGAAATTGGGATGCCAGCTCCTCAACCGAAAGGTACTATCGATTCATTGGCATTGTTGACTCAAAGGAGTTTGGATGATGTCAGGATGAATCTCGAAGTTCTAAAGTATTGTGCAACTGTCTTGTTTCTGGAATCTAGTCTACCCGAAGTTTTCCCGGAGAATAGTTGGGTTTCTCCAGATGCTGTTACAAGGCGTCGTGCTGGAAAATCATCTCCACAAGGAGTTAATTCAAATAATAGTACTCACTCATCAAGTTCAAGGATAGGTGGTAATCCAATATCTCTACCAGATCAACAAGGGGAAGCTCATCCGATATTATCTCTTGTAACTTGCAGCTCTGAGGACGGAACCTCAAATCTAGCTGAATCTGATGCTACTGAATCAGATTCTTTTAACATTCACACTCTCAGTGACCAAATAACAGGTGAATCACTAGAAACAGATATCAGCATGGAAGAAGAAGATGTCTCTGAATCTACTGAAGCATCTCCCACAGAGGATACTTCTACAAGTCTCAGCGCTGCTACTGAATTCTTGGAGCCTGATCAGGTGTCTGTTTCGTCCATCACTGCATCTTTCATTCCATTTCTTCGTGGGAGCCAAAGAATACAATTATCGCACAAAGACGACTGTTTACAGCTTCTTTGTAACAATCTGAAAGTTCGGTTTGGCATCAGCACAAAATTCACTGATTATGCTGGCCGTCCAAGGTTGAGTTTCGTGGTCGATGTACCACCAAGTTTATGCAGTGTTCTTGAAGCATCTGATAGCGTGGCTCAGAGATTATTCTCACAGTCTGGCAGCGGCTCCGAGTGGAGACCTGTCGTGGTGAGACCTGTCGTGGTGAGAAAGAATGGTTATTTCAACTACCCAACAATGAGATTACACATTCCAACTGCAGTGAGTGGAGATACGGCCATTTATGCCACAGAGATGTACCAAAAAGAATCATCAGGTGCAGCACAAAGGCTTATATTTAGTAAATTTGATGCTGCAGAGCTTGATAGCTTGATCAACCCTGGAGCCATCTTAGATGCATTCATTTCCTTGGATACATATGATTATCAACAGAGTGCAGGCATTAGATTGGTAGCAAAAAAGTTGATTATCCATTCCTAG

Coding sequence (CDS)

ATGAGCCGAGTGATCCGTACGTTGGAAGCGCCTTCTTGGCTTCTTCTTCTTCAATCTCAACACAAATCTTCTTCCTTTTCTCTCTCCACCCCTCTGCGCTCTTCTCTGATCGAAATTTCCCTCGCTTCAATCATGGGTCCGACGGAGGATCGATCGGAGATTGCCTTCTTTGATGTGGAGACCACTGTTCCCACCCGCCAAGGCCAGAGATTTTCAATTTTGGAGTTTGGCGCGATTCTGGTGTGCCCTAGAAAGCTGGTTGAGTTGGAGAGCTATTCCACTCTCGTTCGCCCCTCTGATCTCTCTCTCATTTCCTCGTTGTCGGTGCGATGCAATGGCATTACTAGAGACGCCGTCATTTCTTCCCCTACTTTCGCCCAAATTGCCGATAGGGTTTACGATATCCTTCACGGACGGATATGGGCAGGCCATAACATACTGAGATTTGATTGTGCACGAATAAGGGAGGCTTTTGCAGAAATTGGGATGCCAGCTCCTCAACCGAAAGGTACTATCGATTCATTGGCATTGTTGACTCAAAGGAGTTTGGATGATGTCAGGATGAATCTCGAAGTTCTAAAGTATTGTGCAACTGTCTTGTTTCTGGAATCTAGTCTACCCGAAGTTTTCCCGGAGAATAGTTGGGTTTCTCCAGATGCTGTTACAAGGCGTCGTGCTGGAAAATCATCTCCACAAGGAGTTAATTCAAATAATAGTACTCACTCATCAAGTTCAAGGATAGGTGGTAATCCAATATCTCTACCAGATCAACAAGGGGAAGCTCATCCGATATTATCTCTTGTAACTTGCAGCTCTGAGGACGGAACCTCAAATCTAGCTGAATCTGATGCTACTGAATCAGATTCTTTTAACATTCACACTCTCAGTGACCAAATAACAGGTGAATCACTAGAAACAGATATCAGCATGGAAGAAGAAGATGTCTCTGAATCTACTGAAGCATCTCCCACAGAGGATACTTCTACAAGTCTCAGCGCTGCTACTGAATTCTTGGAGCCTGATCAGGTGTCTGTTTCGTCCATCACTGCATCTTTCATTCCATTTCTTCGTGGGAGCCAAAGAATACAATTATCGCACAAAGACGACTGTTTACAGCTTCTTTGTAACAATCTGAAAGTTCGGTTTGGCATCAGCACAAAATTCACTGATTATGCTGGCCGTCCAAGGTTGAGTTTCGTGGTCGATGTACCACCAAGTTTATGCAGTGTTCTTGAAGCATCTGATAGCGTGGCTCAGAGATTATTCTCACAGTCTGGCAGCGGCTCCGAGTGGAGACCTGTCGTGGTGAGACCTGTCGTGGTGAGAAAGAATGGTTATTTCAACTACCCAACAATGAGATTACACATTCCAACTGCAGTGAGTGGAGATACGGCCATTTATGCCACAGAGATGTACCAAAAAGAATCATCAGGTGCAGCACAAAGGCTTATATTTAGTAAATTTGATGCTGCAGAGCTTGATAGCTTGATCAACCCTGGAGCCATCTTAGATGCATTCATTTCCTTGGATACATATGATTATCAACAGAGTGCAGGCATTAGATTGGTAGCAAAAAAGTTGATTATCCATTCCTAG

Protein sequence

MSRVIRTLEAPSWLLLLQSQHKSSSFSLSTPLRSSLIEISLASIMGPTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMPAPQPKGTIDSLALLTQRSLDDVRMNLEVLKYCATVLFLESSLPEVFPENSWVSPDAVTRRRAGKSSPQGVNSNNSTHSSSSRIGGNPISLPDQQGEAHPILSLVTCSSEDGTSNLAESDATESDSFNIHTLSDQITGESLETDISMEEEDVSESTEASPTEDTSTSLSAATEFLEPDQVSVSSITASFIPFLRGSQRIQLSHKDDCLQLLCNNLKVRFGISTKFTDYAGRPRLSFVVDVPPSLCSVLEASDSVAQRLFSQSGSGSEWRPVVVRPVVVRKNGYFNYPTMRLHIPTAVSGDTAIYATEMYQKESSGAAQRLIFSKFDAAELDSLINPGAILDAFISLDTYDYQQSAGIRLVAKKLIIHS

Homology

[Close]

BLAST of Spg022645 vs. NCBI nr
Match: XP_038880150.1 (protein NEN1-like [Benincasa hispida])

HSP 1 Score: 838.2 bits (2164), Expect = 4.0e-239
Identity = 446/512 (87.11%), Postives = 459/512 (89.65%), Query Frame = 0


Query: 45  MGPTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 104
           MG TEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI
Sbjct: 1   MGSTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60

Query: 105 SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 164
           SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIG+P
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120

Query: 165 APQPKGTIDSLALLTQ-------------------------RSLDDVRMNLEVLKYCATV 224
           AP+PKGTIDSLALLTQ                         RSLDDVRMNLEVLKYCATV
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 225 LFLESSLPEVFPENSWVSPDAVTRRRAGKSSPQGVNSNNSTHSSSSRIGGNPISLPDQQG 284
           LFLESSLPEVF ENSWVSP+AVTRRR  KSSPQG +SNN THSSSSRIGGNPISL D QG
Sbjct: 181 LFLESSLPEVFRENSWVSPNAVTRRRTAKSSPQGNSSNNDTHSSSSRIGGNPISLLD-QG 240

Query: 285 EAHPILSLVTCSSEDGTSNLAESDATESDSFNIHTLSDQITGESLETDISMEEEDVSEST 344
           E HPILSLVTCSSEDG SNLAES ATESDSFN+ TLSDQITGESLETDISMEEE  + ST
Sbjct: 241 ETHPILSLVTCSSEDGASNLAESRATESDSFNMRTLSDQITGESLETDISMEEEHATVST 300

Query: 345 EASPTEDTSTSLSAATEFLEPDQVSVSSITASFIPFLRGSQRIQLSHKDDCLQLLCNNLK 404
           EASP+EDTSTSLSA TEFLEPDQVSVS ITASF+PF RGSQRIQLSHKDDCLQLLCNNL+
Sbjct: 301 EASPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQRIQLSHKDDCLQLLCNNLR 360

Query: 405 VRFGISTKFTDYAGRPRLSFVVDVPPSLCSVLEASDSVAQRLFSQSGSGSEWRPVVVRPV 464
           VRFGISTKFTDYAGRP+LSFVVDVPPSLC+VLEASD VAQRLFS SGSGSEW     RPV
Sbjct: 361 VRFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEW-----RPV 420

Query: 465 VVRKNGYFNYPTMRLHIPTAVSGDTAIYATEMYQKESSGAAQRLIFSKFDAAELDSLINP 524
           V+RKN YFNYPTMRLHIPTAVSGD AIYATEMYQKESSGAAQRLIFSKFDAAELDSLI P
Sbjct: 421 VMRKNDYFNYPTMRLHIPTAVSGDVAIYATEMYQKESSGAAQRLIFSKFDAAELDSLIKP 480

Query: 525 GAILDAFISLDTYDYQQSAGIRLVAKKLIIHS 532
           GAILDAFISLDTYDYQQSAGIRLVAK+LIIHS
Sbjct: 481 GAILDAFISLDTYDYQQSAGIRLVAKRLIIHS 506

[Close]

BLAST of Spg022645 vs. NCBI nr
Match: XP_022921575.1 (protein NEN1-like [Cucurbita moschata] >KAG6589518.1 Protein NEN2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 834.7 bits (2155), Expect = 4.4e-238
Identity = 446/527 (84.63%), Postives = 462/527 (87.67%), Query Frame = 0


Query: 45  MGPTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 104
           MGPT DRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKL ELESYSTLVRPSDLSLI
Sbjct: 1   MGPTGDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLDELESYSTLVRPSDLSLI 60

Query: 105 SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 164
           SSLSVRCNGITRDAVIS+PTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP
Sbjct: 61  SSLSVRCNGITRDAVISAPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 120

Query: 165 APQPKGTIDSLALLTQ-------------------------RSLDDVRMNLEVLKYCATV 224
           APQPKGTIDSLALLTQ                         RSLDDVRMNLEVLKYCATV
Sbjct: 121 APQPKGTIDSLALLTQKFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 225 LFLESSLPEVFPENSWVSPDAVTRRRAGKSSPQGVNSNNS--------------THSSSS 284
           LFLESSLPEVFPENSWVSP+AVTRRRA KSSPQG N+NNS              T SSSS
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGTNANNSAAKASPQGINANSNTRSSSS 240

Query: 285 RIGGNPISLPDQQGEAHPILSLVTCSSEDGTSNL-AESDATESDSFNIHTLSDQITGESL 344
           RIGG+PISL DQQGEAHPILSLVTCSSEDG SNL A+SDATESDSFN+ TLSDQI GESL
Sbjct: 241 RIGGSPISLTDQQGEAHPILSLVTCSSEDGASNLVADSDATESDSFNMGTLSDQIAGESL 300

Query: 345 ETDISMEEEDVSESTEASPTEDTSTSLSAATEFLEPDQVSVSSITASFIPFLRGSQRIQL 404
           ETD SMEEE  S STEASPTE+TSTS+S+ATEFLEPDQVSVSSITASF+PF RGSQRIQL
Sbjct: 301 ETDTSMEEEHASVSTEASPTENTSTSISSATEFLEPDQVSVSSITASFVPFFRGSQRIQL 360

Query: 405 SHKDDCLQLLCNNLKVRFGISTKFTDYAGRPRLSFVVDVPPSLCSVLEASDSVAQRLFSQ 464
           SHKDDCLQLLCNNL+VRFGISTKFTDYAGRPRL+FVVDVPPSLC VLEASD VAQRLFS+
Sbjct: 361 SHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLNFVVDVPPSLCRVLEASDGVAQRLFSE 420

Query: 465 SGSGSEWRPVVVRPVVVRKNGYFNYPTMRLHIPTAVSGDTAIYATEMYQKESSGAAQRLI 524
           SGSGSEW     RPVV+RKNGYFNYPTMRLHIPTAVSGD AIYATEMYQKE+SG AQRLI
Sbjct: 421 SGSGSEW-----RPVVMRKNGYFNYPTMRLHIPTAVSGDMAIYATEMYQKEASGGAQRLI 480

Query: 525 FSKFDAAELDSLINPGAILDAFISLDTYDYQQSAGIRLVAKKLIIHS 532
           FSKFDA ELDSLINPG I+DAFISLDTYDYQQSAGIRLVAKKLIIHS
Sbjct: 481 FSKFDAVELDSLINPGGIIDAFISLDTYDYQQSAGIRLVAKKLIIHS 522

[Close]

BLAST of Spg022645 vs. NCBI nr
Match: XP_022987423.1 (protein NEN1-like [Cucurbita maxima])

HSP 1 Score: 833.6 bits (2152), Expect = 9.8e-238
Identity = 446/527 (84.63%), Postives = 460/527 (87.29%), Query Frame = 0


Query: 45  MGPTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 104
           MGPT DRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKL ELESYSTLVRPSDLSLI
Sbjct: 1   MGPTGDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLDELESYSTLVRPSDLSLI 60

Query: 105 SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 164
           SSLSVRCNGITRDAVIS+PTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP
Sbjct: 61  SSLSVRCNGITRDAVISAPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 120

Query: 165 APQPKGTIDSLALLTQ-------------------------RSLDDVRMNLEVLKYCATV 224
           APQPKGTIDSLALLTQ                         RSLDDVRMNLEVLKYCATV
Sbjct: 121 APQPKGTIDSLALLTQKFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 225 LFLESSLPEVFPENSWVSPDAVTRRRAGKSSPQGVNSNNS--------------THSSSS 284
           LFLESSLPEVFPENSWVSP+AVTRRRA KSSPQG+N NNS              T SSSS
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGINENNSAAKSSPRGINANSNTRSSSS 240

Query: 285 RIGGNPISLPDQQGEAHPILSLVTCSSEDGTSNL-AESDATESDSFNIHTLSDQITGESL 344
           RIGG+PISL DQQGEAHPILSLVTCSSEDG SNL A+SDATESDSFN+ TLSDQI GESL
Sbjct: 241 RIGGSPISLTDQQGEAHPILSLVTCSSEDGASNLVADSDATESDSFNMGTLSDQIAGESL 300

Query: 345 ETDISMEEEDVSESTEASPTEDTSTSLSAATEFLEPDQVSVSSITASFIPFLRGSQRIQL 404
           ETD SMEEE    STEASPTE+TSTSLSAATEFLEPDQVSVSSITASF+PF RGSQRIQL
Sbjct: 301 ETDTSMEEEHAPVSTEASPTENTSTSLSAATEFLEPDQVSVSSITASFVPFFRGSQRIQL 360

Query: 405 SHKDDCLQLLCNNLKVRFGISTKFTDYAGRPRLSFVVDVPPSLCSVLEASDSVAQRLFSQ 464
           SHKDDCLQLLCNNL+VRFGISTKFTDYAGRPRL+FVVDVPPSLC VLEASD VAQRLFS+
Sbjct: 361 SHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLNFVVDVPPSLCRVLEASDGVAQRLFSE 420

Query: 465 SGSGSEWRPVVVRPVVVRKNGYFNYPTMRLHIPTAVSGDTAIYATEMYQKESSGAAQRLI 524
           SGSGSEW     RPVV+RKNGYFNYPTMRLHIPTAV GD AIYATEMYQKE+SG AQRLI
Sbjct: 421 SGSGSEW-----RPVVMRKNGYFNYPTMRLHIPTAVCGDMAIYATEMYQKEASGGAQRLI 480

Query: 525 FSKFDAAELDSLINPGAILDAFISLDTYDYQQSAGIRLVAKKLIIHS 532
           FSKFDA ELDSLINPG I+DAFISLDTYDYQQSAGIRLVAKKLIIHS
Sbjct: 481 FSKFDAVELDSLINPGGIIDAFISLDTYDYQQSAGIRLVAKKLIIHS 522

[Close]

BLAST of Spg022645 vs. NCBI nr
Match: XP_023515370.1 (protein NEN1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 830.5 bits (2144), Expect = 8.3e-237
Identity = 444/527 (84.25%), Postives = 461/527 (87.48%), Query Frame = 0


Query: 45  MGPTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 104
           MGPT DRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKL ELESYSTLVRPSDLSLI
Sbjct: 1   MGPTGDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLDELESYSTLVRPSDLSLI 60

Query: 105 SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 164
           SSLSVRCNGITRDAVIS+PTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP
Sbjct: 61  SSLSVRCNGITRDAVISAPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 120

Query: 165 APQPKGTIDSLALLTQ-------------------------RSLDDVRMNLEVLKYCATV 224
           AP+PKGTIDSLALLTQ                         RSLDDVRMNLEVLKYCATV
Sbjct: 121 APEPKGTIDSLALLTQKFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 225 LFLESSLPEVFPENSWVSPDAVTRRRAGKSSPQGVNSNNS--------------THSSSS 284
           LFLESSLPEVFPENSW+SP+AVTRRRA KSSPQG+N+NNS              T SSSS
Sbjct: 181 LFLESSLPEVFPENSWISPNAVTRRRAAKSSPQGINANNSAAKSSPQGINANSNTRSSSS 240

Query: 285 RIGGNPISLPDQQGEAHPILSLVTCSSEDGTSNL-AESDATESDSFNIHTLSDQITGESL 344
           RIGG+PISL DQQGEAHPILSLVTCSSEDG SNL A+SDATESDSFN+ TLSDQI GESL
Sbjct: 241 RIGGSPISLTDQQGEAHPILSLVTCSSEDGASNLVADSDATESDSFNMGTLSDQIAGESL 300

Query: 345 ETDISMEEEDVSESTEASPTEDTSTSLSAATEFLEPDQVSVSSITASFIPFLRGSQRIQL 404
           ET  SMEEE    STEASPTE+TSTSLSAATEFLEPDQVSVSSITASF+PF RGSQRIQL
Sbjct: 301 ETYSSMEEEHAPVSTEASPTENTSTSLSAATEFLEPDQVSVSSITASFVPFFRGSQRIQL 360

Query: 405 SHKDDCLQLLCNNLKVRFGISTKFTDYAGRPRLSFVVDVPPSLCSVLEASDSVAQRLFSQ 464
           SHKDDCLQLLCNNL+VRFGISTKFTDYAGRPRL+FVVDVPPSLC VLEASD VAQRLFS+
Sbjct: 361 SHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLNFVVDVPPSLCRVLEASDGVAQRLFSE 420

Query: 465 SGSGSEWRPVVVRPVVVRKNGYFNYPTMRLHIPTAVSGDTAIYATEMYQKESSGAAQRLI 524
           SGSGSEW     RPVV+RKNGYFNYPTMRLHIPTAVSGD AIYATEMYQKE+SG AQRLI
Sbjct: 421 SGSGSEW-----RPVVMRKNGYFNYPTMRLHIPTAVSGDMAIYATEMYQKEASGGAQRLI 480

Query: 525 FSKFDAAELDSLINPGAILDAFISLDTYDYQQSAGIRLVAKKLIIHS 532
           FSKFDA ELDSLINPG I+DAFISLDTYDYQQSAGIRLVAKKLIIHS
Sbjct: 481 FSKFDAVELDSLINPGGIIDAFISLDTYDYQQSAGIRLVAKKLIIHS 522

[Close]

BLAST of Spg022645 vs. NCBI nr
Match: XP_023515369.1 (protein NEN1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 825.1 bits (2130), Expect = 3.5e-235
Identity = 444/541 (82.07%), Postives = 461/541 (85.21%), Query Frame = 0


Query: 45  MGPTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 104
           MGPT DRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKL ELESYSTLVRPSDLSLI
Sbjct: 1   MGPTGDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLDELESYSTLVRPSDLSLI 60

Query: 105 SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 164
           SSLSVRCNGITRDAVIS+PTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP
Sbjct: 61  SSLSVRCNGITRDAVISAPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 120

Query: 165 APQPKGTIDSLALLTQ-------------------------RSLDDVRMNLEVLKYCATV 224
           AP+PKGTIDSLALLTQ                         RSLDDVRMNLEVLKYCATV
Sbjct: 121 APEPKGTIDSLALLTQKFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 225 LFLESSLPEVFPENSWVSPDAVTRRRAGKSSPQGVNSNNS-------------------- 284
           LFLESSLPEVFPENSW+SP+AVTRRRA KSSPQG+N+NNS                    
Sbjct: 181 LFLESSLPEVFPENSWISPNAVTRRRAAKSSPQGINANNSAAKSSPQGINANNSAAKSSP 240

Query: 285 --------THSSSSRIGGNPISLPDQQGEAHPILSLVTCSSEDGTSNL-AESDATESDSF 344
                   T SSSSRIGG+PISL DQQGEAHPILSLVTCSSEDG SNL A+SDATESDSF
Sbjct: 241 QGINANSNTRSSSSRIGGSPISLTDQQGEAHPILSLVTCSSEDGASNLVADSDATESDSF 300

Query: 345 NIHTLSDQITGESLETDISMEEEDVSESTEASPTEDTSTSLSAATEFLEPDQVSVSSITA 404
           N+ TLSDQI GESLET  SMEEE    STEASPTE+TSTSLSAATEFLEPDQVSVSSITA
Sbjct: 301 NMGTLSDQIAGESLETYSSMEEEHAPVSTEASPTENTSTSLSAATEFLEPDQVSVSSITA 360

Query: 405 SFIPFLRGSQRIQLSHKDDCLQLLCNNLKVRFGISTKFTDYAGRPRLSFVVDVPPSLCSV 464
           SF+PF RGSQRIQLSHKDDCLQLLCNNL+VRFGISTKFTDYAGRPRL+FVVDVPPSLC V
Sbjct: 361 SFVPFFRGSQRIQLSHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLNFVVDVPPSLCRV 420

Query: 465 LEASDSVAQRLFSQSGSGSEWRPVVVRPVVVRKNGYFNYPTMRLHIPTAVSGDTAIYATE 524
           LEASD VAQRLFS+SGSGSEW     RPVV+RKNGYFNYPTMRLHIPTAVSGD AIYATE
Sbjct: 421 LEASDGVAQRLFSESGSGSEW-----RPVVMRKNGYFNYPTMRLHIPTAVSGDMAIYATE 480

Query: 525 MYQKESSGAAQRLIFSKFDAAELDSLINPGAILDAFISLDTYDYQQSAGIRLVAKKLIIH 532
           MYQKE+SG AQRLIFSKFDA ELDSLINPG I+DAFISLDTYDYQQSAGIRLVAKKLIIH
Sbjct: 481 MYQKEASGGAQRLIFSKFDAVELDSLINPGGIIDAFISLDTYDYQQSAGIRLVAKKLIIH 536

[Close]

BLAST of Spg022645 vs. ExPASy Swiss-Prot
Match: Q9FLR0 (Protein NEN1 OS=Arabidopsis thaliana OX=3702 GN=NEN1 PE=2 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 1.6e-129
Identity = 274/511 (53.62%), Postives = 344/511 (67.32%), Query Frame = 0


Query: 49  EDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLS 108
           EDRSEIAFFDVETTVP R GQRF+ILEFG+ILVCP+KL EL SY+TLV+P+DLSLISSLS
Sbjct: 4   EDRSEIAFFDVETTVPKR-GQRFAILEFGSILVCPKKLTELRSYTTLVQPADLSLISSLS 63

Query: 109 VRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMPAPQP 168
           VRCNGI RD V+ +P FA IAD VYDILHGRIWAGHNILRFDCARIREAFAEIG   P+P
Sbjct: 64  VRCNGIKRDDVVLAPLFADIADTVYDILHGRIWAGHNILRFDCARIREAFAEIGRQPPEP 123

Query: 169 KGTIDSLALLTQ-------------------------RSLDDVRMNLEVLKYCATVLFLE 228
           KG IDSL LLTQ                         RSLDDVRMNLEVLKYCATVLFLE
Sbjct: 124 KGAIDSLGLLTQKFGRRAGDMKMATLARYFGLGNQTHRSLDDVRMNLEVLKYCATVLFLE 183

Query: 229 SSLPEVFPENSWVSPDAVTRRRAGKSSPQGVNSNNSTHSSSSRIGGNPISLPDQQGEAHP 288
           SSLP    +NS VSP+ ++ RR   +S +G     +T ++S R+                
Sbjct: 184 SSLPYAHVDNS-VSPETISSRRRIDASREG-----NTVTTSVRL---------------- 243

Query: 289 ILSLVTCSSEDGTSNLAESDATESDSFNIHTLSDQITGES-LETDISMEEEDVSESTEAS 348
                         +++E+ A + D FN+  L +++  ++ +++DI MEEE + E ++  
Sbjct: 244 -------------PSISENSAAQPDPFNMSVLRNEMASDNHIQSDILMEEEQI-EPSDVV 303

Query: 349 PTEDTSTSLSAATEFLEPDQVSVSSITASFIPFLRGSQ--RIQLSHKDDCLQLLCNNLKV 408
            +E+TS        FL PD +S+S+I A   PF  GSQ  +++L H D  LQL C+ LK+
Sbjct: 304 ASENTSDH----EGFLTPDAMSLSNIKAMLFPFYPGSQMMKLKLLHGDSPLQLYCSYLKI 363

Query: 409 RFGISTKFTDYAGRPRLSFVVDVPPSLCSVLEASDSVAQRLFSQSGSGSEWRPVVVRPVV 468
           RFG++ KF D  GR RL+FVVD+ PSL S+LEA DS AQ+L   SGS SEW P VV P+ 
Sbjct: 364 RFGVNGKFLDNTGRRRLNFVVDLNPSLYSILEACDSNAQKLSVDSGSTSEWNP-VVNPM- 423

Query: 469 VRKNGYFNYPTMRLHIPTAVSGDTAIYATEMYQKESSGAAQRLIFSKFDAAELDSLINPG 528
               G+ NYP  R+HI T ++GD A YATE++Q+ESSGA Q+LIFS  +  EL+SL+  G
Sbjct: 424 ---KGFVNYPNARIHIATEINGDEARYATEIHQRESSGATQKLIFSNPNNEELESLLTTG 468

Query: 529 AILDAFISLDTYDYQQSAGIRLVAKKLIIHS 532
           +++DAF+SL+ YDYQQ AGIRLVAKKL+I S
Sbjct: 484 SVVDAFLSLEPYDYQQKAGIRLVAKKLVIQS 468

[Close]

BLAST of Spg022645 vs. ExPASy Swiss-Prot
Match: Q9CA74 (Protein NEN3 OS=Arabidopsis thaliana OX=3702 GN=NEN3 PE=2 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 4.7e-126
Identity = 260/517 (50.29%), Postives = 345/517 (66.73%), Query Frame = 0


Query: 41  LASIMGPTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSD 100
           +AS +G  ++RSEIAFFD+ET VPT+ G+ F+ILEFGAILVCPR+L EL SYSTLVRP+D
Sbjct: 1   MASSLG-GDERSEIAFFDLETAVPTKSGEPFAILEFGAILVCPRRLEELYSYSTLVRPTD 60

Query: 101 LSLISSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAE 160
           LSLIS+L+ R +GITRD V+S+ TF++IAD+VYDILHGRIWAGHNI+RFDC RIREAFAE
Sbjct: 61  LSLISTLTKRRSGITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAE 120

Query: 161 IGMPAPQPKGTIDSLALLTQ-------------------------RSLDDVRMNLEVLKY 220
           IG+  P+PK TIDSL+LL+Q                         RSLDDVRMNLEV+KY
Sbjct: 121 IGLSPPEPKATIDSLSLLSQKFGKRAGDMKMASLATYFGLGDQAHRSLDDVRMNLEVVKY 180

Query: 221 CATVLFLESSLPEVFPENSWVSPDAVTRRRA-GKSSPQGVNSNNSTHSSSSRIGGNPISL 280
           CATVLFLESS+P++  + SW SP    R R+ GK    GV  ++++ SSS +   +  S+
Sbjct: 181 CATVLFLESSVPDILTDMSWFSPRKSPRTRSNGKLVANGVRESSTSSSSSPKTDPSSSSV 240

Query: 281 PDQQGEAHPILSLVT-CSSEDGTSNLAESDATESDSFNIHTLSDQITGESLETDISMEEE 340
                + HPI+SL+T CS  D +S   E      D  +I TL  ++   +L+TD + E +
Sbjct: 241 DATIVKNHPIVSLLTECSESDASSYDIE------DPIDITTLIGKLRIGTLQTDAAEEAK 300

Query: 341 DVSESTEASPTEDTSTSLSAATEFLEPDQVSVSSITASFIPFLRGSQRIQLSHKDDCLQL 400
            V +  E+ P+ D+    +    FL  ++VSVSSI AS +PF RGS R++L H D  L L
Sbjct: 301 TVRQQDESPPSPDSD---AKDESFLGVNEVSVSSIRASLVPFYRGSLRMKLFHNDTPLHL 360

Query: 401 LCNNLKVRFGISTKFTDYAGRPRLSFVVDVPPSLCSVLEASDSVAQRLFSQSGSGSEWRP 460
             ++LKVRFGIS KF D+AGRP+L+ +VD P  LC +L+A D+ A  L + S + S+W  
Sbjct: 361 CWHSLKVRFGISRKFVDHAGRPKLNIIVDAPLDLCKILDAVDAAAHNLPTDSSTNSDW-- 420

Query: 461 VVVRPVVVRKNGYFNYPTMRLHIPTAVSGDTAIYATEMYQKESS-GAAQRLIFSKFDAAE 520
              RP V+RK G+ NYPT RLHI +  +GD  +  T++YQKE   G  Q+L  S  +  +
Sbjct: 421 ---RPTVIRKEGFANYPTARLHISSESNGDDTLCGTQVYQKEEPLGTNQKLDVSSDNLEK 480

Query: 521 LDSLINPGAILDAFISLDTYDYQQSAGIRLVAKKLII 530
           L+S + PG ++DAF SL+ Y YQQ AGIRL  KKL+I
Sbjct: 481 LESALLPGTLVDAFFSLEPYSYQQMAGIRLAVKKLVI 502

[Close]

BLAST of Spg022645 vs. ExPASy Swiss-Prot
Match: Q0V842 (Protein NEN2 OS=Arabidopsis thaliana OX=3702 GN=NEN2 PE=2 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 1.5e-124
Identity = 268/516 (51.94%), Postives = 336/516 (65.12%), Query Frame = 0


Query: 47  PTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISS 106
           P EDRSEIAFFDVETT+P R GQ ++ILEFG+ILVCP+KLVEL++YS LVRP++L+LI+ 
Sbjct: 9   PAEDRSEIAFFDVETTIPFRVGQGYAILEFGSILVCPKKLVELKNYSVLVRPANLNLITP 68

Query: 107 LSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMPAP 166
            SV+CNGI R+ V S+ TFA IAD VYDILHGRIWAGHNIL+FDC RIREAFAEIG   P
Sbjct: 69  RSVKCNGIKREDVESALTFADIADTVYDILHGRIWAGHNILKFDCPRIREAFAEIGRDPP 128

Query: 167 QPKGTIDSLALLTQ-------------------------RSLDDVRMNLEVLKYCATVLF 226
           +PKGTIDSLALLTQ                         RSLDDVRMN EVLKYCATVLF
Sbjct: 129 EPKGTIDSLALLTQRFGRRAGDMKMATLATYFGLGNQTHRSLDDVRMNFEVLKYCATVLF 188

Query: 227 LESSLPEVFPENSWVSPDAVT---RRRAGKSSPQGVNSNNSTHSSSSRIGGNPISLPDQQ 286
           LESSLP+   ENS  +    T   RRR  K SP    ++  T  + + I           
Sbjct: 189 LESSLPDELIENSVTTTTPETSSRRRRTIKKSPLQSPTDQQTGENMTTI----------- 248

Query: 287 GEAHPILSLVTCSSEDGTSNLAESDATESDSFNIHTLSDQITGESLETDISMEEEDVSES 346
               PILS V+            S   ++D F++ TL ++I  E L++D+ MEEE  ++ 
Sbjct: 249 ----PILSFVS------------SAEAQTDPFDMSTLRNEIAPEVLQSDVPMEEEQ-NQQ 308

Query: 347 TEASPTEDTSTSLSAATEFLEPDQVSVSSITASFIPFLRGSQ--RIQLSHKDDCLQLLCN 406
           +E   +E T         F+E D++SVSSI A+ +P   GSQ  ++QL   D  LQL C 
Sbjct: 309 SETVASEGTGDQ----EGFMELDKISVSSIRATHVPLYDGSQTMKLQLFLGDRPLQLHCP 368

Query: 407 NLKVRFGISTKFTDYAGRPRLSFVVDVPPSLCSVLEASDSVAQRLFSQSGSGSEWRPVVV 466
            LKVRFGI+ KF D AGR RL+FV+D+ PSLC+VL+  DS AQ +   SGSGS+W     
Sbjct: 369 RLKVRFGINGKFMDKAGRRRLNFVIDLYPSLCNVLQECDSAAQTISVDSGSGSDW----- 428

Query: 467 RPVVVRKNGYFNYPTMRLHIPTAVSGDTAIYATEMYQKESSG--AAQRLIFSKFDAAELD 526
            P+V+   G+ N PT R+HIPT ++GD   YA E++QKE SG  A Q+LI S   A E++
Sbjct: 429 NPLVIPMKGFLNCPTARIHIPTELNGDIDRYAAEIHQKEFSGATATQKLISSNPKAEEIE 487

Query: 527 SLINPGAILDAFISLDTYDYQQSAGIRLVAKKLIIH 531
           SL+NP  +LDAF+SL+ YDYQQ AGIRLVA+KL+IH
Sbjct: 489 SLLNPRTVLDAFLSLEPYDYQQRAGIRLVARKLVIH 487

[Close]

BLAST of Spg022645 vs. ExPASy Swiss-Prot
Match: F4JJ23 (Protein NEN4 OS=Arabidopsis thaliana OX=3702 GN=NEN4 PE=2 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 1.5e-52
Identity = 107/199 (53.77%), Postives = 135/199 (67.84%), Query Frame = 0


Query: 52  SEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLSVRC 111
           +EI FFD+ET VP + GQ F ILEFGAI+VCP+KL ELES++TL++P DLS++S  S R 
Sbjct: 8   NEIVFFDLETNVPNKAGQHFHILEFGAIIVCPKKLEELESFTTLIQPKDLSVVSIRSSRS 67

Query: 112 NGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMPAPQPKGT 171
           +GITR  V ++P+F  +A++++ +L+GRIWAGHNI RFDC RI+EAFAEIG  AP+P G 
Sbjct: 68  DGITRAKVTNAPSFEDVAEKIHGLLNGRIWAGHNIRRFDCVRIKEAFAEIGKAAPEPSGI 127

Query: 172 IDSLALLT-------------------------QRSLDDVRMNLEVLKYCATVLFLESSL 226
           IDSL LL+                          RSLDDVRMNLEVLK+CATVLFLES+L
Sbjct: 128 IDSLGLLSDKFGKRAGNMKMASLAAYFGLGVQKHRSLDDVRMNLEVLKHCATVLFLESTL 187

[Close]

BLAST of Spg022645 vs. ExPASy TrEMBL
Match: A0A6J1E665 (protein NEN1-like OS=Cucurbita moschata OX=3662 GN=LOC111429799 PE=4 SV=1)

HSP 1 Score: 834.7 bits (2155), Expect = 2.1e-238
Identity = 446/527 (84.63%), Postives = 462/527 (87.67%), Query Frame = 0


Query: 45  MGPTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 104
           MGPT DRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKL ELESYSTLVRPSDLSLI
Sbjct: 1   MGPTGDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLDELESYSTLVRPSDLSLI 60

Query: 105 SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 164
           SSLSVRCNGITRDAVIS+PTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP
Sbjct: 61  SSLSVRCNGITRDAVISAPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 120

Query: 165 APQPKGTIDSLALLTQ-------------------------RSLDDVRMNLEVLKYCATV 224
           APQPKGTIDSLALLTQ                         RSLDDVRMNLEVLKYCATV
Sbjct: 121 APQPKGTIDSLALLTQKFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 225 LFLESSLPEVFPENSWVSPDAVTRRRAGKSSPQGVNSNNS--------------THSSSS 284
           LFLESSLPEVFPENSWVSP+AVTRRRA KSSPQG N+NNS              T SSSS
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGTNANNSAAKASPQGINANSNTRSSSS 240

Query: 285 RIGGNPISLPDQQGEAHPILSLVTCSSEDGTSNL-AESDATESDSFNIHTLSDQITGESL 344
           RIGG+PISL DQQGEAHPILSLVTCSSEDG SNL A+SDATESDSFN+ TLSDQI GESL
Sbjct: 241 RIGGSPISLTDQQGEAHPILSLVTCSSEDGASNLVADSDATESDSFNMGTLSDQIAGESL 300

Query: 345 ETDISMEEEDVSESTEASPTEDTSTSLSAATEFLEPDQVSVSSITASFIPFLRGSQRIQL 404
           ETD SMEEE  S STEASPTE+TSTS+S+ATEFLEPDQVSVSSITASF+PF RGSQRIQL
Sbjct: 301 ETDTSMEEEHASVSTEASPTENTSTSISSATEFLEPDQVSVSSITASFVPFFRGSQRIQL 360

Query: 405 SHKDDCLQLLCNNLKVRFGISTKFTDYAGRPRLSFVVDVPPSLCSVLEASDSVAQRLFSQ 464
           SHKDDCLQLLCNNL+VRFGISTKFTDYAGRPRL+FVVDVPPSLC VLEASD VAQRLFS+
Sbjct: 361 SHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLNFVVDVPPSLCRVLEASDGVAQRLFSE 420

Query: 465 SGSGSEWRPVVVRPVVVRKNGYFNYPTMRLHIPTAVSGDTAIYATEMYQKESSGAAQRLI 524
           SGSGSEW     RPVV+RKNGYFNYPTMRLHIPTAVSGD AIYATEMYQKE+SG AQRLI
Sbjct: 421 SGSGSEW-----RPVVMRKNGYFNYPTMRLHIPTAVSGDMAIYATEMYQKEASGGAQRLI 480

Query: 525 FSKFDAAELDSLINPGAILDAFISLDTYDYQQSAGIRLVAKKLIIHS 532
           FSKFDA ELDSLINPG I+DAFISLDTYDYQQSAGIRLVAKKLIIHS
Sbjct: 481 FSKFDAVELDSLINPGGIIDAFISLDTYDYQQSAGIRLVAKKLIIHS 522

[Close]

BLAST of Spg022645 vs. ExPASy TrEMBL
Match: A0A6J1JJE4 (protein NEN1-like OS=Cucurbita maxima OX=3661 GN=LOC111484975 PE=4 SV=1)

HSP 1 Score: 833.6 bits (2152), Expect = 4.7e-238
Identity = 446/527 (84.63%), Postives = 460/527 (87.29%), Query Frame = 0


Query: 45  MGPTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 104
           MGPT DRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKL ELESYSTLVRPSDLSLI
Sbjct: 1   MGPTGDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLDELESYSTLVRPSDLSLI 60

Query: 105 SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 164
           SSLSVRCNGITRDAVIS+PTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP
Sbjct: 61  SSLSVRCNGITRDAVISAPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 120

Query: 165 APQPKGTIDSLALLTQ-------------------------RSLDDVRMNLEVLKYCATV 224
           APQPKGTIDSLALLTQ                         RSLDDVRMNLEVLKYCATV
Sbjct: 121 APQPKGTIDSLALLTQKFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 225 LFLESSLPEVFPENSWVSPDAVTRRRAGKSSPQGVNSNNS--------------THSSSS 284
           LFLESSLPEVFPENSWVSP+AVTRRRA KSSPQG+N NNS              T SSSS
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGINENNSAAKSSPRGINANSNTRSSSS 240

Query: 285 RIGGNPISLPDQQGEAHPILSLVTCSSEDGTSNL-AESDATESDSFNIHTLSDQITGESL 344
           RIGG+PISL DQQGEAHPILSLVTCSSEDG SNL A+SDATESDSFN+ TLSDQI GESL
Sbjct: 241 RIGGSPISLTDQQGEAHPILSLVTCSSEDGASNLVADSDATESDSFNMGTLSDQIAGESL 300

Query: 345 ETDISMEEEDVSESTEASPTEDTSTSLSAATEFLEPDQVSVSSITASFIPFLRGSQRIQL 404
           ETD SMEEE    STEASPTE+TSTSLSAATEFLEPDQVSVSSITASF+PF RGSQRIQL
Sbjct: 301 ETDTSMEEEHAPVSTEASPTENTSTSLSAATEFLEPDQVSVSSITASFVPFFRGSQRIQL 360

Query: 405 SHKDDCLQLLCNNLKVRFGISTKFTDYAGRPRLSFVVDVPPSLCSVLEASDSVAQRLFSQ 464
           SHKDDCLQLLCNNL+VRFGISTKFTDYAGRPRL+FVVDVPPSLC VLEASD VAQRLFS+
Sbjct: 361 SHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLNFVVDVPPSLCRVLEASDGVAQRLFSE 420

Query: 465 SGSGSEWRPVVVRPVVVRKNGYFNYPTMRLHIPTAVSGDTAIYATEMYQKESSGAAQRLI 524
           SGSGSEW     RPVV+RKNGYFNYPTMRLHIPTAV GD AIYATEMYQKE+SG AQRLI
Sbjct: 421 SGSGSEW-----RPVVMRKNGYFNYPTMRLHIPTAVCGDMAIYATEMYQKEASGGAQRLI 480

Query: 525 FSKFDAAELDSLINPGAILDAFISLDTYDYQQSAGIRLVAKKLIIHS 532
           FSKFDA ELDSLINPG I+DAFISLDTYDYQQSAGIRLVAKKLIIHS
Sbjct: 481 FSKFDAVELDSLINPGGIIDAFISLDTYDYQQSAGIRLVAKKLIIHS 522

[Close]

BLAST of Spg022645 vs. ExPASy TrEMBL
Match: A0A5A7UTA8 (Protein NEN1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002600 PE=4 SV=1)

HSP 1 Score: 820.1 bits (2117), Expect = 5.4e-234
Identity = 431/511 (84.34%), Postives = 454/511 (88.85%), Query Frame = 0


Query: 45  MGPTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 104
           MGPTEDRSEIAFFDVETTVPTRQGQ+FSILEFGAILVCP+KLVELESYSTLV+PSDLSLI
Sbjct: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60

Query: 105 SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 164
           SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFA+IG+P
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFADIGVP 120

Query: 165 APQPKGTIDSLALLTQR------------------------SLDDVRMNLEVLKYCATVL 224
           AP+PKGTIDSLALLTQR                        SLDDVRMNLEVLKYCATVL
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKVMTEFSLLHANSGDQSLDDVRMNLEVLKYCATVL 180

Query: 225 FLESSLPEVFPENSWVSPDAVTRRRAGKSSPQGVNSNNSTHSSSSRIGGNPISLPDQQGE 284
           FLESSLPEVFPENSWVSP+AVTRRRA KSSPQG +SNN++H+SSS IGGNPISL DQQGE
Sbjct: 181 FLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISLTDQQGE 240

Query: 285 AHPILSLVTCSSEDGTSNLAESDATESDSFNIHTLSDQITGESLETDISMEEEDVSESTE 344
           AHPILSLVTCSSEDG  NLAE  ATESDSFN+HT+SDQITG +LETDI+ME E  + STE
Sbjct: 241 AHPILSLVTCSSEDGALNLAEPSATESDSFNMHTISDQITGATLETDINMEGEHATVSTE 300

Query: 345 ASPTEDTSTSLSAATEFLEPDQVSVSSITASFIPFLRGSQRIQLSHKDDCLQLLCNNLKV 404
           ASP+EDTSTSL   T+FLEPDQVSV  ITASF+PF RGSQRIQL HKDDCLQLLCNNL+V
Sbjct: 301 ASPSEDTSTSLH-TTKFLEPDQVSVLFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRV 360

Query: 405 RFGISTKFTDYAGRPRLSFVVDVPPSLCSVLEASDSVAQRLFSQSGSGSEWRPVVVRPVV 464
           RFGISTKFTDYAGRPRLSFVVDVPPSLC+VLEASD VAQRLFS SGSGSEW     RP V
Sbjct: 361 RFGISTKFTDYAGRPRLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEW-----RPAV 420

Query: 465 VRKNGYFNYPTMRLHIPTAVSGDTAIYATEMYQKESSGAAQRLIFSKFDAAELDSLINPG 524
            RKNGYFNYPTMRLHIPTAVSGD A YATEMYQK++SGA QRLIFSKFDAAELDSLI PG
Sbjct: 421 TRKNGYFNYPTMRLHIPTAVSGDVANYATEMYQKDASGAVQRLIFSKFDAAELDSLIKPG 480

Query: 525 AILDAFISLDTYDYQQSAGIRLVAKKLIIHS 532
           AILDAFISLDTYDYQQSAGIRLVAKKLIIHS
Sbjct: 481 AILDAFISLDTYDYQQSAGIRLVAKKLIIHS 505

[Close]

BLAST of Spg022645 vs. ExPASy TrEMBL
Match: A0A1S3BWV4 (protein NEN1 OS=Cucumis melo OX=3656 GN=LOC103494007 PE=4 SV=1)

HSP 1 Score: 819.7 bits (2116), Expect = 7.1e-234
Identity = 431/512 (84.18%), Postives = 454/512 (88.67%), Query Frame = 0


Query: 45  MGPTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 104
           MGPTEDRSEIAFFDVETTVPTRQGQ+FSILEFGAILVCP+KLVELESYSTLV+PSDLSLI
Sbjct: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60

Query: 105 SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 164
           SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFA+IG+P
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFADIGVP 120

Query: 165 APQPKGTIDSLALLTQ-------------------------RSLDDVRMNLEVLKYCATV 224
           AP+PKGTIDSLALLTQ                         RSLDDVRMNLEVLKYCATV
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 225 LFLESSLPEVFPENSWVSPDAVTRRRAGKSSPQGVNSNNSTHSSSSRIGGNPISLPDQQG 284
           LFLESSLPEVFPENSWVSP+AVTRRRA KSSPQG +SNN++H+SSS IGGNPISL DQQG
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISLTDQQG 240

Query: 285 EAHPILSLVTCSSEDGTSNLAESDATESDSFNIHTLSDQITGESLETDISMEEEDVSEST 344
           EAHPILSLVTCSSEDG  NLAE  ATESDSFN+HT+SDQITG +LETDI+ME E  + ST
Sbjct: 241 EAHPILSLVTCSSEDGALNLAEPSATESDSFNMHTISDQITGATLETDINMEGEHATVST 300

Query: 345 EASPTEDTSTSLSAATEFLEPDQVSVSSITASFIPFLRGSQRIQLSHKDDCLQLLCNNLK 404
           EASP+EDTSTSL   T+FLEPDQVSV  ITASF+PF RGSQRIQL HKDDCLQLLCNNL+
Sbjct: 301 EASPSEDTSTSLH-TTKFLEPDQVSVLFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLR 360

Query: 405 VRFGISTKFTDYAGRPRLSFVVDVPPSLCSVLEASDSVAQRLFSQSGSGSEWRPVVVRPV 464
           VRFGISTKFTDYAGRPRLSFVVDVPPSLC+VLEASD VAQRLFS SGSGSEW     RP 
Sbjct: 361 VRFGISTKFTDYAGRPRLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEW-----RPA 420

Query: 465 VVRKNGYFNYPTMRLHIPTAVSGDTAIYATEMYQKESSGAAQRLIFSKFDAAELDSLINP 524
           V RKNGYFNYPTMRLHIPTAVSGD A YATEMYQK++SGA QRLIFSKFDAAELDSLI P
Sbjct: 421 VTRKNGYFNYPTMRLHIPTAVSGDVANYATEMYQKDASGAVQRLIFSKFDAAELDSLIKP 480

Query: 525 GAILDAFISLDTYDYQQSAGIRLVAKKLIIHS 532
           GAILDAFISLDTYDYQQSAGIRLVAKKLIIHS
Sbjct: 481 GAILDAFISLDTYDYQQSAGIRLVAKKLIIHS 506

[Close]

BLAST of Spg022645 vs. ExPASy TrEMBL
Match: A0A0A0LSC3 (Exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G009910 PE=4 SV=1)

HSP 1 Score: 802.7 bits (2072), Expect = 9.0e-229
Identity = 427/512 (83.40%), Postives = 449/512 (87.70%), Query Frame = 0


Query: 45  MGPTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 104
           MGPTEDRSEIAFFDVETTVPTRQGQ+FSILEFGAILVCP+KLVELESYSTLV+PSDLSLI
Sbjct: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60

Query: 105 SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 164
           SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIG+P
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120

Query: 165 APQPKGTIDSLALLTQ-------------------------RSLDDVRMNLEVLKYCATV 224
           AP+PKGTIDSLALLTQ                         RSLDDVRMNLEVLKYCATV
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 225 LFLESSLPEVFPENSWVSPDAVTRRRAGKSSPQGVNSNNSTHSSSSRIGGNPISLPDQQG 284
           LFLESSLPEVFPENSWVSP+AVTRRRA KSSPQG +SNN++H+SSS IGGNPISLP QQG
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISLPVQQG 240

Query: 285 EAHPILSLVTCSSEDGTSNLAESDATESDSFNIHTLSDQITGESLETDISMEEEDVSEST 344
           E HPILSLVT  SED  S LAE  ATESDSFN+HT SDQITG +LETDI+MEE   + ST
Sbjct: 241 ETHPILSLVTICSEDRASILAEPSATESDSFNMHTTSDQITGATLETDINMEEH-ATVST 300

Query: 345 EASPTEDTSTSLSAATEFLEPDQVSVSSITASFIPFLRGSQRIQLSHKDDCLQLLCNNLK 404
           EASP+EDTSTSL   T+FLEPDQVSVS ITASF+PF RGSQRIQL HKDDCLQLLCNNL+
Sbjct: 301 EASPSEDTSTSL-CTTKFLEPDQVSVSFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLR 360

Query: 405 VRFGISTKFTDYAGRPRLSFVVDVPPSLCSVLEASDSVAQRLFSQSGSGSEWRPVVVRPV 464
           VRFGISTKFTDYAGRPRLSFVVDVPP+LC+VLEASD VAQRLFS SGSGSEW     RP 
Sbjct: 361 VRFGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEW-----RPA 420

Query: 465 VVRKNGYFNYPTMRLHIPTAVSGDTAIYATEMYQKESSGAAQRLIFSKFDAAELDSLINP 524
           V RKNGYFNYPTMRLHIPTAVSGD A YATEM+QKE+SGA QRLIFSKFDAAELDSLI P
Sbjct: 421 VTRKNGYFNYPTMRLHIPTAVSGDVANYATEMHQKEASGAVQRLIFSKFDAAELDSLIKP 480

Query: 525 GAILDAFISLDTYDYQQSAGIRLVAKKLIIHS 532
           GAILDAFISLDTYDYQQSAGIRLVAKKLII S
Sbjct: 481 GAILDAFISLDTYDYQQSAGIRLVAKKLIIRS 505

[Close]

BLAST of Spg022645 vs. TAIR 10
Match: AT5G07710.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 464.5 bits (1194), Expect = 1.1e-130
Identity = 274/511 (53.62%), Postives = 344/511 (67.32%), Query Frame = 0


Query: 49  EDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLS 108
           EDRSEIAFFDVETTVP R GQRF+ILEFG+ILVCP+KL EL SY+TLV+P+DLSLISSLS
Sbjct: 4   EDRSEIAFFDVETTVPKR-GQRFAILEFGSILVCPKKLTELRSYTTLVQPADLSLISSLS 63

Query: 109 VRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMPAPQP 168
           VRCNGI RD V+ +P FA IAD VYDILHGRIWAGHNILRFDCARIREAFAEIG   P+P
Sbjct: 64  VRCNGIKRDDVVLAPLFADIADTVYDILHGRIWAGHNILRFDCARIREAFAEIGRQPPEP 123

Query: 169 KGTIDSLALLTQ-------------------------RSLDDVRMNLEVLKYCATVLFLE 228
           KG IDSL LLTQ                         RSLDDVRMNLEVLKYCATVLFLE
Sbjct: 124 KGAIDSLGLLTQKFGRRAGDMKMATLARYFGLGNQTHRSLDDVRMNLEVLKYCATVLFLE 183

Query: 229 SSLPEVFPENSWVSPDAVTRRRAGKSSPQGVNSNNSTHSSSSRIGGNPISLPDQQGEAHP 288
           SSLP    +NS VSP+ ++ RR   +S +G     +T ++S R+                
Sbjct: 184 SSLPYAHVDNS-VSPETISSRRRIDASREG-----NTVTTSVRL---------------- 243

Query: 289 ILSLVTCSSEDGTSNLAESDATESDSFNIHTLSDQITGES-LETDISMEEEDVSESTEAS 348
                         +++E+ A + D FN+  L +++  ++ +++DI MEEE + E ++  
Sbjct: 244 -------------PSISENSAAQPDPFNMSVLRNEMASDNHIQSDILMEEEQI-EPSDVV 303

Query: 349 PTEDTSTSLSAATEFLEPDQVSVSSITASFIPFLRGSQ--RIQLSHKDDCLQLLCNNLKV 408
            +E+TS        FL PD +S+S+I A   PF  GSQ  +++L H D  LQL C+ LK+
Sbjct: 304 ASENTSDH----EGFLTPDAMSLSNIKAMLFPFYPGSQMMKLKLLHGDSPLQLYCSYLKI 363

Query: 409 RFGISTKFTDYAGRPRLSFVVDVPPSLCSVLEASDSVAQRLFSQSGSGSEWRPVVVRPVV 468
           RFG++ KF D  GR RL+FVVD+ PSL S+LEA DS AQ+L   SGS SEW P VV P+ 
Sbjct: 364 RFGVNGKFLDNTGRRRLNFVVDLNPSLYSILEACDSNAQKLSVDSGSTSEWNP-VVNPM- 423

Query: 469 VRKNGYFNYPTMRLHIPTAVSGDTAIYATEMYQKESSGAAQRLIFSKFDAAELDSLINPG 528
               G+ NYP  R+HI T ++GD A YATE++Q+ESSGA Q+LIFS  +  EL+SL+  G
Sbjct: 424 ---KGFVNYPNARIHIATEINGDEARYATEIHQRESSGATQKLIFSNPNNEELESLLTTG 468

Query: 529 AILDAFISLDTYDYQQSAGIRLVAKKLIIHS 532
           +++DAF+SL+ YDYQQ AGIRLVAKKL+I S
Sbjct: 484 SVVDAFLSLEPYDYQQKAGIRLVAKKLVIQS 468

[Close]

BLAST of Spg022645 vs. TAIR 10
Match: AT1G74390.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 453.0 bits (1164), Expect = 3.4e-127
Identity = 260/517 (50.29%), Postives = 345/517 (66.73%), Query Frame = 0


Query: 41  LASIMGPTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSD 100
           +AS +G  ++RSEIAFFD+ET VPT+ G+ F+ILEFGAILVCPR+L EL SYSTLVRP+D
Sbjct: 1   MASSLG-GDERSEIAFFDLETAVPTKSGEPFAILEFGAILVCPRRLEELYSYSTLVRPTD 60

Query: 101 LSLISSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAE 160
           LSLIS+L+ R +GITRD V+S+ TF++IAD+VYDILHGRIWAGHNI+RFDC RIREAFAE
Sbjct: 61  LSLISTLTKRRSGITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAE 120

Query: 161 IGMPAPQPKGTIDSLALLTQ-------------------------RSLDDVRMNLEVLKY 220
           IG+  P+PK TIDSL+LL+Q                         RSLDDVRMNLEV+KY
Sbjct: 121 IGLSPPEPKATIDSLSLLSQKFGKRAGDMKMASLATYFGLGDQAHRSLDDVRMNLEVVKY 180

Query: 221 CATVLFLESSLPEVFPENSWVSPDAVTRRRA-GKSSPQGVNSNNSTHSSSSRIGGNPISL 280
           CATVLFLESS+P++  + SW SP    R R+ GK    GV  ++++ SSS +   +  S+
Sbjct: 181 CATVLFLESSVPDILTDMSWFSPRKSPRTRSNGKLVANGVRESSTSSSSSPKTDPSSSSV 240

Query: 281 PDQQGEAHPILSLVT-CSSEDGTSNLAESDATESDSFNIHTLSDQITGESLETDISMEEE 340
                + HPI+SL+T CS  D +S   E      D  +I TL  ++   +L+TD + E +
Sbjct: 241 DATIVKNHPIVSLLTECSESDASSYDIE------DPIDITTLIGKLRIGTLQTDAAEEAK 300

Query: 341 DVSESTEASPTEDTSTSLSAATEFLEPDQVSVSSITASFIPFLRGSQRIQLSHKDDCLQL 400
            V +  E+ P+ D+    +    FL  ++VSVSSI AS +PF RGS R++L H D  L L
Sbjct: 301 TVRQQDESPPSPDSD---AKDESFLGVNEVSVSSIRASLVPFYRGSLRMKLFHNDTPLHL 360

Query: 401 LCNNLKVRFGISTKFTDYAGRPRLSFVVDVPPSLCSVLEASDSVAQRLFSQSGSGSEWRP 460
             ++LKVRFGIS KF D+AGRP+L+ +VD P  LC +L+A D+ A  L + S + S+W  
Sbjct: 361 CWHSLKVRFGISRKFVDHAGRPKLNIIVDAPLDLCKILDAVDAAAHNLPTDSSTNSDW-- 420

Query: 461 VVVRPVVVRKNGYFNYPTMRLHIPTAVSGDTAIYATEMYQKESS-GAAQRLIFSKFDAAE 520
              RP V+RK G+ NYPT RLHI +  +GD  +  T++YQKE   G  Q+L  S  +  +
Sbjct: 421 ---RPTVIRKEGFANYPTARLHISSESNGDDTLCGTQVYQKEEPLGTNQKLDVSSDNLEK 480

Query: 521 LDSLINPGAILDAFISLDTYDYQQSAGIRLVAKKLII 530
           L+S + PG ++DAF SL+ Y YQQ AGIRL  KKL+I
Sbjct: 481 LESALLPGTLVDAFFSLEPYSYQQMAGIRLAVKKLVI 502

[Close]

BLAST of Spg022645 vs. TAIR 10
Match: AT5G61390.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 448.0 bits (1151), Expect = 1.1e-125
Identity = 268/516 (51.94%), Postives = 336/516 (65.12%), Query Frame = 0


Query: 47  PTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISS 106
           P EDRSEIAFFDVETT+P R GQ ++ILEFG+ILVCP+KLVEL++YS LVRP++L+LI+ 
Sbjct: 9   PAEDRSEIAFFDVETTIPFRVGQGYAILEFGSILVCPKKLVELKNYSVLVRPANLNLITP 68

Query: 107 LSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMPAP 166
            SV+CNGI R+ V S+ TFA IAD VYDILHGRIWAGHNIL+FDC RIREAFAEIG   P
Sbjct: 69  RSVKCNGIKREDVESALTFADIADTVYDILHGRIWAGHNILKFDCPRIREAFAEIGRDPP 128

Query: 167 QPKGTIDSLALLTQ-------------------------RSLDDVRMNLEVLKYCATVLF 226
           +PKGTIDSLALLTQ                         RSLDDVRMN EVLKYCATVLF
Sbjct: 129 EPKGTIDSLALLTQRFGRRAGDMKMATLATYFGLGNQTHRSLDDVRMNFEVLKYCATVLF 188

Query: 227 LESSLPEVFPENSWVSPDAVT---RRRAGKSSPQGVNSNNSTHSSSSRIGGNPISLPDQQ 286
           LESSLP+   ENS  +    T   RRR  K SP    ++  T  + + I           
Sbjct: 189 LESSLPDELIENSVTTTTPETSSRRRRTIKKSPLQSPTDQQTGENMTTI----------- 248

Query: 287 GEAHPILSLVTCSSEDGTSNLAESDATESDSFNIHTLSDQITGESLETDISMEEEDVSES 346
               PILS V+            S   ++D F++ TL ++I  E L++D+ MEEE  ++ 
Sbjct: 249 ----PILSFVS------------SAEAQTDPFDMSTLRNEIAPEVLQSDVPMEEEQ-NQQ 308

Query: 347 TEASPTEDTSTSLSAATEFLEPDQVSVSSITASFIPFLRGSQ--RIQLSHKDDCLQLLCN 406
           +E   +E T         F+E D++SVSSI A+ +P   GSQ  ++QL   D  LQL C 
Sbjct: 309 SETVASEGTGDQ----EGFMELDKISVSSIRATHVPLYDGSQTMKLQLFLGDRPLQLHCP 368

Query: 407 NLKVRFGISTKFTDYAGRPRLSFVVDVPPSLCSVLEASDSVAQRLFSQSGSGSEWRPVVV 466
            LKVRFGI+ KF D AGR RL+FV+D+ PSLC+VL+  DS AQ +   SGSGS+W     
Sbjct: 369 RLKVRFGINGKFMDKAGRRRLNFVIDLYPSLCNVLQECDSAAQTISVDSGSGSDW----- 428

Query: 467 RPVVVRKNGYFNYPTMRLHIPTAVSGDTAIYATEMYQKESSG--AAQRLIFSKFDAAELD 526
            P+V+   G+ N PT R+HIPT ++GD   YA E++QKE SG  A Q+LI S   A E++
Sbjct: 429 NPLVIPMKGFLNCPTARIHIPTELNGDIDRYAAEIHQKEFSGATATQKLISSNPKAEEIE 487

Query: 527 SLINPGAILDAFISLDTYDYQQSAGIRLVAKKLIIH 531
           SL+NP  +LDAF+SL+ YDYQQ AGIRLVA+KL+IH
Sbjct: 489 SLLNPRTVLDAFLSLEPYDYQQRAGIRLVARKLVIH 487

[Close]

BLAST of Spg022645 vs. TAIR 10
Match: AT1G74390.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 424.5 bits (1090), Expect = 1.3e-118
Identity = 250/517 (48.36%), Postives = 334/517 (64.60%), Query Frame = 0


Query: 41  LASIMGPTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSD 100
           +AS +G  ++RSEIAFFD+ET VPT+ G+ F+ILEFGAILVCPR+L EL SYSTLVRP+D
Sbjct: 1   MASSLG-GDERSEIAFFDLETAVPTKSGEPFAILEFGAILVCPRRLEELYSYSTLVRPTD 60

Query: 101 LSLISSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAE 160
           LSLIS+L+ R +GITRD V+S+ TF++IAD+VYDILHGRIWAGHNI+RFDC RIREAFAE
Sbjct: 61  LSLISTLTKRRSGITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAE 120

Query: 161 IGMPAPQPKGTIDSLALLTQ-------------------------RSLDDVRMNLEVLKY 220
           IG+  P+PK TIDSL+LL+Q                         RSLDDVRMNLE    
Sbjct: 121 IGLSPPEPKATIDSLSLLSQKFGKRAGDMKMASLATYFGLGDQAHRSLDDVRMNLE---- 180

Query: 221 CATVLFLESSLPEVFPENSWVSPDAVTRRRA-GKSSPQGVNSNNSTHSSSSRIGGNPISL 280
                  ESS+P++  + SW SP    R R+ GK    GV  ++++ SSS +   +  S+
Sbjct: 181 -------ESSVPDILTDMSWFSPRKSPRTRSNGKLVANGVRESSTSSSSSPKTDPSSSSV 240

Query: 281 PDQQGEAHPILSLVT-CSSEDGTSNLAESDATESDSFNIHTLSDQITGESLETDISMEEE 340
                + HPI+SL+T CS  D +S   E      D  +I TL  ++   +L+TD + E +
Sbjct: 241 DATIVKNHPIVSLLTECSESDASSYDIE------DPIDITTLIGKLRIGTLQTDAAEEAK 300

Query: 341 DVSESTEASPTEDTSTSLSAATEFLEPDQVSVSSITASFIPFLRGSQRIQLSHKDDCLQL 400
            V +  E+ P+ D+    +    FL  ++VSVSSI AS +PF RGS R++L H D  L L
Sbjct: 301 TVRQQDESPPSPDSD---AKDESFLGVNEVSVSSIRASLVPFYRGSLRMKLFHNDTPLHL 360

Query: 401 LCNNLKVRFGISTKFTDYAGRPRLSFVVDVPPSLCSVLEASDSVAQRLFSQSGSGSEWRP 460
             ++LKVRFGIS KF D+AGRP+L+ +VD P  LC +L+A D+ A  L + S + S+W  
Sbjct: 361 CWHSLKVRFGISRKFVDHAGRPKLNIIVDAPLDLCKILDAVDAAAHNLPTDSSTNSDW-- 420

Query: 461 VVVRPVVVRKNGYFNYPTMRLHIPTAVSGDTAIYATEMYQKESS-GAAQRLIFSKFDAAE 520
              RP V+RK G+ NYPT RLHI +  +GD  +  T++YQKE   G  Q+L  S  +  +
Sbjct: 421 ---RPTVIRKEGFANYPTARLHISSESNGDDTLCGTQVYQKEEPLGTNQKLDVSSDNLEK 480

Query: 521 LDSLINPGAILDAFISLDTYDYQQSAGIRLVAKKLII 530
           L+S + PG ++DAF SL+ Y YQQ AGIRL  KKL+I
Sbjct: 481 LESALLPGTLVDAFFSLEPYSYQQMAGIRLAVKKLVI 491

[Close]

BLAST of Spg022645 vs. TAIR 10
Match: AT4G39810.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 208.8 bits (530), Expect = 1.1e-53
Identity = 107/199 (53.77%), Postives = 135/199 (67.84%), Query Frame = 0


Query: 52  SEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLSVRC 111
           +EI FFD+ET VP + GQ F ILEFGAI+VCP+KL ELES++TL++P DLS++S  S R 
Sbjct: 8   NEIVFFDLETNVPNKAGQHFHILEFGAIIVCPKKLEELESFTTLIQPKDLSVVSIRSSRS 67

Query: 112 NGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMPAPQPKGT 171
           +GITR  V ++P+F  +A++++ +L+GRIWAGHNI RFDC RI+EAFAEIG  AP+P G 
Sbjct: 68  DGITRAKVTNAPSFEDVAEKIHGLLNGRIWAGHNIRRFDCVRIKEAFAEIGKAAPEPSGI 127

Query: 172 IDSLALLT-------------------------QRSLDDVRMNLEVLKYCATVLFLESSL 226
           IDSL LL+                          RSLDDVRMNLEVLK+CATVLFLES+L
Sbjct: 128 IDSLGLLSDKFGKRAGNMKMASLAAYFGLGVQKHRSLDDVRMNLEVLKHCATVLFLESTL 187

The following BLAST results are available for this feature:

BLAST of Spg022645 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038880150.1	4.0e-239	87.11	protein NEN1-like [Benincasa hispida]	[more]
XP_022921575.1	4.4e-238	84.63	protein NEN1-like [Cucurbita moschata] >KAG6589518.1 Protein NEN2, partial [Cucu...	[more]
XP_022987423.1	9.8e-238	84.63	protein NEN1-like [Cucurbita maxima]	[more]
XP_023515370.1	8.3e-237	84.25	protein NEN1-like isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_023515369.1	3.5e-235	82.07	protein NEN1-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Spg022645 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FLR0	1.6e-129	53.62	Protein NEN1 OS=Arabidopsis thaliana OX=3702 GN=NEN1 PE=2 SV=1	[more]
Q9CA74	4.7e-126	50.29	Protein NEN3 OS=Arabidopsis thaliana OX=3702 GN=NEN3 PE=2 SV=1	[more]
Q0V842	1.5e-124	51.94	Protein NEN2 OS=Arabidopsis thaliana OX=3702 GN=NEN2 PE=2 SV=1	[more]
F4JJ23	1.5e-52	53.77	Protein NEN4 OS=Arabidopsis thaliana OX=3702 GN=NEN4 PE=2 SV=1	[more]

BLAST of Spg022645 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E665	2.1e-238	84.63	protein NEN1-like OS=Cucurbita moschata OX=3662 GN=LOC111429799 PE=4 SV=1	[more]
A0A6J1JJE4	4.7e-238	84.63	protein NEN1-like OS=Cucurbita maxima OX=3661 GN=LOC111484975 PE=4 SV=1	[more]
A0A5A7UTA8	5.4e-234	84.34	Protein NEN1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002600 ...	[more]
A0A1S3BWV4	7.1e-234	84.18	protein NEN1 OS=Cucumis melo OX=3656 GN=LOC103494007 PE=4 SV=1	[more]
A0A0A0LSC3	9.0e-229	83.40	Exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G009910...	[more]

BLAST of Spg022645 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G07710.1	1.1e-130	53.62	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G74390.1	3.4e-127	50.29	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT5G61390.1	1.1e-125	51.94	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G74390.2	1.3e-118	48.36	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT4G39810.1	1.1e-53	53.77	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013520	Exonuclease, RNase T/DNA polymerase III	SMART	SM00479	exoiiiendus	coord: 53..202 e-value: 1.2E-16 score: 71.3
IPR013520	Exonuclease, RNase T/DNA polymerase III	PFAM	PF00929	RNase_T	coord: 56..181 e-value: 2.9E-14 score: 53.9
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 48..204 e-value: 2.6E-21 score: 78.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 309..331
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 228..252
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 221..258
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 273..296
None	No IPR available	PANTHER	PTHR30231:SF27	PROTEIN NEN3	coord: 179..530 coord: 42..181
None	No IPR available	PANTHER	PTHR30231	DNA POLYMERASE III SUBUNIT EPSILON	coord: 179..530
None	No IPR available	PANTHER	PTHR30231	DNA POLYMERASE III SUBUNIT EPSILON	coord: 42..181
None	No IPR available	CDD	cd06127	DEDDh	coord: 55..194 e-value: 3.80078E-21 score: 88.1264
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 53..200

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg022645.1	Spg022645.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
molecular_function	GO:0008408	3'-5' exonuclease activity
molecular_function	GO:0003676	nucleic acid binding