Spg022644 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg022644
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	protein EMBRYO DEFECTIVE 514
Location	scaffold2: 4920937 .. 4924088 (-)
RNA-Seq Expression	Spg022644
Synteny	Spg022644

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TAAAAAAAAAAAAAAAAAAACGGTTTACATAGACCGATCCGTGTAGAAATTAAACCGGACACCTTCAATTTAAAGATGAAGGGGCGAATATAAAATCTCGTCTTCTGTATTGCTAGGGTTTTAGCTTTTAAGCTTTGCGGTTCCAACTTCAGAGTAACTTAAAATGGCAGAAGAGACAACCCCTCAACTCCCTGAAAACGATCCGGAGGTTACCAACTCGGCCACCGAAGATATGGAACTAGAAAGCACAGAGCCTGGGGCAGAGACGAAAGCTAACAACGGTGATTCTGAAGCAAATGAGGCCGCTCCAGTAACGAATGGAGATGCAAGTTCGAAGCGAGAGAGGGAGGAAGAAAGCACTGATGACAGCGTCGAAGAGGCGAAAAAGCAGAAGGTGGAGAAGTCAGTTGAAGAGGAAAGGCTTGAGAAGGCGGAAGGAGATGGGAAAGCAGAGGAGACTGGTCCTGTGAGTTTAGGCCCGAAGAGCTTTGGATCATCGGTGGAGTTGTTCGATTACTTCTACAGTTTCCTTCACCACTGGCCCGCCAATTTGAACGTGAACCAGGTATGATATTATGGACTTCTTTTATTTGCTATCATCCTCTGCTTTGTTAATGGAAAGGATGGGAATAACATCGATAAAACTTTATTTTAGTTAGAAGGGGGATTTGGAACTCGGTTGAAAGAAAATTTCTACTAAATGCTGCTGGTTTTGTTCATCATCATCGTTATTTTATTGTGTGAAAGACACATATCGCCAAAGAAGAAGAAAAAAAAATTCAAAATTAGGTGGGGAACTAGGATCTTTATCCGCATGAAAGGTGAATGGTTCATTAGTTAGCCGAAGGTGACTCTTGTTTGAACCCGTTTCTTATGTCAATCAATTTGATATAAATCTTTTAGTATCTTTTTAGCTTTTAAGTATCATGAATTACTGTCTTAGTAGGTTGGGTCTATAATCTATTATGGAGGCAAGTCACTTAGTACTTAATTATCGTAGTTGTCTTGTTGGAGAAAAGGCTTGTATTTGTGGGCAAGTTGGAATCTGAGACGTGTCATTCTTCAATATTCTCATGGAGATTGATGATGGTTGCTTCTTAACTTTTAAAAGTTTGAGGTCTTTACTTCTCAGAGTTCCTTAATAGGCACACTGAATGTGTATTATTTTATTTTATTTTTCTCTTTAAGTCGTATTATATTTTTTGATCGACAATATTTCTTGGCAAGCAACCGGGCGAGTATTTGAAATTATGATCAGCATCAGTTTATCATGAAAGGTTTATTTAATTGGTCCTGTATTCGGTGAAGATATTATTTGTCTGCCTCTGCATGTTTATTTTAAAGGAGATCTCTAAACTTCTTGCTTGGTGGATTATTCAACGCATCATGTGATTGCGTTTATTTTAATTGAAAACCAAAAGATCATTTCAGTGCTTCTATCAGATTGATTAATTAGTGGTTGGCTTGATTTTATACAATTTATAAAAAAAAAGTATACTGGTTGGTTTGGTTGTGATCCGTTTTGTTGATTTTTTAGTCAATTCTAGTTGATCTTCTGGATGGTTTTTCCAAAAAAAAAATATATATATATAGAAGTTTGATTTTTCAGTTAGTTCCCAATTTTTTCTAAACTCTGTTGATTTGTTGTCAGTTTTGGTCAAAAACCCACTTCTTTAGATGGGAATGTTTGACATAATTGAACATGGGAAATGGAATAATTGTGGCACTTATAAAACCCCCACCCTGAAAGGATAAATTTTATTTGTTTCTTTGAATGTATACTCTTTTGGCATGAGTTGCTCATTGAAGAATTTCTTTCAGCAGCTTATTCTGCATTGTTGTATGTTATTCTTCATAATTCCCTTCTTATTCTTGATTGATTTTAGTGGAAGAAATGGATATGCTTTATCTATTTAGTTCTTCTGGCATATCCTTTGATGTAGAAAGTATGGCATGGCTCTTCAGAAGCAAGAGCCGCACTAAGCCAATGTGCCTCTATAGGATAATTTTGCAGTCCAAAGGGGGAAGTTATGGTTAGTGTTTGGGAACCCATGTATTCTTGGCCGGTTACAGGTTGTGATCCCATGAGGAAATCATTCTGAAATAATACTTAAATCAAAAGGCAGTTAAGTGTTATTCTTTGAATTCCGCAGCAATTATTAGTACTTCTGTTTTATCTTCTTCCAATATTCTTGCTTTCTGTCTAAATCTTGCTTCCCATAGGTATTATTCAAATGCATATGCTTTTGCCAATCTTAACATAGCTCAAATGGTTAAAACGTTATTAACTTTCTTTGTAGGTTCAATTGTATGCTTGAATTGTAATCTTAAGAAAAAGAAGTGTGTACCATTAACGCTCCCAGTATTATTTTCAGTAATGTTTTCCATTCAGTCAGTGCCGTTCCAAGGGCAAAATTTTCCACCATCTTTAGTCCGGAATGTCTTCAACTTGATATGAAACATTTATTGTTGATCCAATTTAATGTTGAAGAAATTGATTCGTGGTAAAAATATTTACATGTTGAGGAAGCATACACTCCATAGATGACCAATATTTGATAATTGTTTCTGTGGCAGTACGAGAAGATGGTGCTGCTGGATTTGATTAAGAAGGGCCATGTAGAACCGGAGAAAAAGATCGGCTGTGGTATTAATGCTTTCCAAATTCGATACCATCCTATGTGGAAAAGCAAATGCTTCTTCCTCATTCGAGAGGACGAGTCTTCTGATGACTTCAGCTTCAGGAAGTGTGTGGATCACATCCTTCCCCTGCCTGAAAACTTGAAGGCAAAATCTGATGCAAACAGGGCATTAGGAGGTGGTAAGCACCGCGGAGGAGGACGAGGCGGTGGACGTGGAAGAGGGGGTAGGTGGAGAAACTGATGATTTTCCCAAGTTCTTTCAGTTTCAGCACGTTTGAGTCAGTTTTCCTGCGATGAATGTGCGACCAAATCCCAACCATGTCGCTCTTGGCCTCGAGTTCAAGGAAGAAAGTAGAGTAATAGTTTGTTTATGCCCATAATTTCTGTTTTTTATGCTTCTTTACCCGTGCTGATGTGATCTTTGAACTCAAAATTAGAGAATATACTTTCATAACGAATAATTATGAAAGGATGAGTCTTTACTTTATTTTAAAGAGAAATCATTTCTTC

mRNA sequence

ATGGCAGAAGAGACAACCCCTCAACTCCCTGAAAACGATCCGGAGGTTACCAACTCGGCCACCGAAGATATGGAACTAGAAAGCACAGAGCCTGGGGCAGAGACGAAAGCTAACAACGGTGATTCTGAAGCAAATGAGGCCGCTCCAGTAACGAATGGAGATGCAAGTTCGAAGCGAGAGAGGGAGGAAGAAAGCACTGATGACAGCGTCGAAGAGGCGAAAAAGCAGAAGGTGGAGAAGTCAGTTGAAGAGGAAAGGCTTGAGAAGGCGGAAGGAGATGGGAAAGCAGAGGAGACTGGTCCTGTGAGTTTAGGCCCGAAGAGCTTTGGATCATCGGTGGAGTTGTTCGATTACTTCTACAGTTTCCTTCACCACTGGCCCGCCAATTTGAACGTGAACCAGGATAATTTTGCAGTCCAAAGGGGGAAGTTATGGTTAGTGTTTGGGAACCCATGTATTCTTGGCCGGTTACAGTACGAGAAGATGGTGCTGCTGGATTTGATTAAGAAGGGCCATGTAGAACCGGAGAAAAAGATCGGCTGTGGTATTAATGCTTTCCAAATTCGATACCATCCTATGTGGAAAAGCAAATGCTTCTTCCTCATTCGAGAGGACGAGTCTTCTGATGACTTCAGCTTCAGGAAGTGTGTGGATCACATCCTTCCCCTGCCTGAAAACTTGAAGGCAAAATCTGATGCAAACAGGGCATTAGGAGGTGGTAAGCACCGCGGAGGAGGACGAGGCGGTGGACGTGGAAGAGGGGGTAGGTGGAGAAACTGA

Coding sequence (CDS)

ATGGCAGAAGAGACAACCCCTCAACTCCCTGAAAACGATCCGGAGGTTACCAACTCGGCCACCGAAGATATGGAACTAGAAAGCACAGAGCCTGGGGCAGAGACGAAAGCTAACAACGGTGATTCTGAAGCAAATGAGGCCGCTCCAGTAACGAATGGAGATGCAAGTTCGAAGCGAGAGAGGGAGGAAGAAAGCACTGATGACAGCGTCGAAGAGGCGAAAAAGCAGAAGGTGGAGAAGTCAGTTGAAGAGGAAAGGCTTGAGAAGGCGGAAGGAGATGGGAAAGCAGAGGAGACTGGTCCTGTGAGTTTAGGCCCGAAGAGCTTTGGATCATCGGTGGAGTTGTTCGATTACTTCTACAGTTTCCTTCACCACTGGCCCGCCAATTTGAACGTGAACCAGGATAATTTTGCAGTCCAAAGGGGGAAGTTATGGTTAGTGTTTGGGAACCCATGTATTCTTGGCCGGTTACAGTACGAGAAGATGGTGCTGCTGGATTTGATTAAGAAGGGCCATGTAGAACCGGAGAAAAAGATCGGCTGTGGTATTAATGCTTTCCAAATTCGATACCATCCTATGTGGAAAAGCAAATGCTTCTTCCTCATTCGAGAGGACGAGTCTTCTGATGACTTCAGCTTCAGGAAGTGTGTGGATCACATCCTTCCCCTGCCTGAAAACTTGAAGGCAAAATCTGATGCAAACAGGGCATTAGGAGGTGGTAAGCACCGCGGAGGAGGACGAGGCGGTGGACGTGGAAGAGGGGGTAGGTGGAGAAACTGA

Protein sequence

MAEETTPQLPENDPEVTNSATEDMELESTEPGAETKANNGDSEANEAAPVTNGDASSKREREEESTDDSVEEAKKQKVEKSVEEERLEKAEGDGKAEETGPVSLGPKSFGSSVELFDYFYSFLHHWPANLNVNQDNFAVQRGKLWLVFGNPCILGRLQYEKMVLLDLIKKGHVEPEKKIGCGINAFQIRYHPMWKSKCFFLIREDESSDDFSFRKCVDHILPLPENLKAKSDANRALGGGKHRGGGRGGGRGRGGRWRN

Homology

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BLAST of Spg022644 vs. NCBI nr
Match: XP_022135230.1 (protein EMBRYO DEFECTIVE 514 [Momordica charantia])

HSP 1 Score: 356.3 bits (913), Expect = 2.2e-94
Identity = 198/262 (75.57%), Postives = 218/262 (83.21%), Query Frame = 0


Query: 1   MAEETTPQLPENDPEVTNSATEDMELESTEPGAETKANNGDSEANEAAPVTNGDASSKRE 60
           MAEETTPQL ENDPEVTNSATEDMELE++EPGAE K  NGDSEANEAAP TNGDA+SKRE
Sbjct: 1   MAEETTPQLAENDPEVTNSATEDMELETSEPGAEPKGLNGDSEANEAAPETNGDANSKRE 60

Query: 61  REEESTDDSVEEAKKQKVEKSVEEERLEKAEGDGKAEE-TGPVSLGPKSFGSSVELFDYF 120
           REEE+ D+++ EAKK KVEKSVEEER+EK EGDGK +E +GPVSLGPKSFGSSVE+FDYF
Sbjct: 61  REEETADENIGEAKKPKVEKSVEEERMEKVEGDGKGDEKSGPVSLGPKSFGSSVEMFDYF 120

Query: 121 YSFLHHWPANLNVNQDNFAVQRGKLWLVFGNPCILGRLQYEKMVLLDLIKKGHVEPEKKI 180
           Y FLH+WPANLN+N                        +YE+MVLL+L+KKGHVEPEKKI
Sbjct: 121 YKFLHYWPANLNLN------------------------EYEQMVLLELLKKGHVEPEKKI 180

Query: 181 GCGINAFQIRYHPMWKSKCFFLIREDESSDDFSFRKCVDHILPLPENLKAKSDANRALGG 240
           GCGI+AFQIR HP +KSKC+FLIREDES DDFSFRKCVDHILPLPENLK KSDANRALGG
Sbjct: 181 GCGIHAFQIRNHPTFKSKCYFLIREDESVDDFSFRKCVDHILPLPENLKVKSDANRALGG 238

Query: 241 GKHRGGGR--GGGRGRGGRWRN 260
            KHRGGGR  GGGRGRGGR RN
Sbjct: 241 CKHRGGGRGGGGGRGRGGRSRN 238

[Close]

BLAST of Spg022644 vs. NCBI nr
Match: XP_022933555.1 (protein EMBRYO DEFECTIVE 514-like [Cucurbita moschata])

HSP 1 Score: 347.8 bits (891), Expect = 7.9e-92
Identity = 197/260 (75.77%), Postives = 205/260 (78.85%), Query Frame = 0


Query: 1   MAEETTPQLPENDPEVTNSATEDMELESTEPGAETKANNGDSEANEAAPVTNGDASSKRE 60
           MAEET PQL EN PEVTNSA EDMELESTEP    K + GDS+ANEAAPVTNGDA+SKRE
Sbjct: 1   MAEETIPQLAENGPEVTNSAAEDMELESTEP----KVSGGDSDANEAAPVTNGDANSKRE 60

Query: 61  REEESTDDSVEEAKKQKVEKSVEEERLEKAEGDGKAEET-GPVSLGPKSFGSSVELFDYF 120
           REEE  DD   EAKK KVEKSVEEERLEK E DGK EE  GPVSLGPKSFGSSVELFDYF
Sbjct: 61  REEEGADDHAGEAKKPKVEKSVEEERLEKVERDGKGEEEHGPVSLGPKSFGSSVELFDYF 120

Query: 121 YSFLHHWPANLNVNQDNFAVQRGKLWLVFGNPCILGRLQYEKMVLLDLIKKGHVEPEKKI 180
           Y+FLH+WPANLNVN                        QYE MVLLDLIKKGHVEP+KKI
Sbjct: 121 YNFLHYWPANLNVN------------------------QYELMVLLDLIKKGHVEPDKKI 180

Query: 181 GCGINAFQIRYHPMWKSKCFFLIREDESSDDFSFRKCVDHILPLPENLKAKSDANRALGG 240
           GCG+ A QIRYHPMWKSKCFFLIRED S+DDFSFRKCVDHILPLPENLK  SDANRALGG
Sbjct: 181 GCGVRACQIRYHPMWKSKCFFLIREDGSADDFSFRKCVDHILPLPENLKTISDANRALGG 228

Query: 241 GKHRGGGRGGGRGRGGRWRN 260
           GKHRGGGRGG    GGRWRN
Sbjct: 241 GKHRGGGRGG----GGRWRN 228

[Close]

BLAST of Spg022644 vs. NCBI nr
Match: KAG7021514.1 (Protein EMBRYO DEFECTIVE [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 347.4 bits (890), Expect = 1.0e-91
Identity = 197/260 (75.77%), Postives = 204/260 (78.46%), Query Frame = 0


Query: 1   MAEETTPQLPENDPEVTNSATEDMELESTEPGAETKANNGDSEANEAAPVTNGDASSKRE 60
           MAEET PQL EN PEVTNSA EDMELESTEP    K + GDSEANEAAPVTNGDA+SKRE
Sbjct: 1   MAEETIPQLAENGPEVTNSAAEDMELESTEP----KVSGGDSEANEAAPVTNGDANSKRE 60

Query: 61  REEESTDDSVEEAKKQKVEKSVEEERLEKAEGDGKAEET-GPVSLGPKSFGSSVELFDYF 120
           REEE  DD   EAKK KVEKSVEEERLEK E DGK EE  GPVSLGPKSFGSSVELFDYF
Sbjct: 61  REEEGADDHAGEAKKPKVEKSVEEERLEKVERDGKGEEEHGPVSLGPKSFGSSVELFDYF 120

Query: 121 YSFLHHWPANLNVNQDNFAVQRGKLWLVFGNPCILGRLQYEKMVLLDLIKKGHVEPEKKI 180
           Y+FLH+WPANLNVN                        QYE MVLLDLIKKGHVEP+KKI
Sbjct: 121 YNFLHYWPANLNVN------------------------QYELMVLLDLIKKGHVEPDKKI 180

Query: 181 GCGINAFQIRYHPMWKSKCFFLIREDESSDDFSFRKCVDHILPLPENLKAKSDANRALGG 240
           GCG+ A QIRYHPMWKSKCFFLIRED S+DDFSFRKCVDHILPLPENLK  SDANRALGG
Sbjct: 181 GCGVRACQIRYHPMWKSKCFFLIREDGSADDFSFRKCVDHILPLPENLKTISDANRALGG 227

Query: 241 GKHRGGGRGGGRGRGGRWRN 260
           GKHRGGGRG     GGRWRN
Sbjct: 241 GKHRGGGRG-----GGRWRN 227

[Close]

BLAST of Spg022644 vs. NCBI nr
Match: XP_023529405.1 (protein EMBRYO DEFECTIVE 514-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 344.7 bits (883), Expect = 6.7e-91
Identity = 196/260 (75.38%), Postives = 205/260 (78.85%), Query Frame = 0


Query: 1   MAEETTPQLPENDPEVTNSATEDMELESTEPGAETKANNGDSEANEAAPVTNGDASSKRE 60
           MAEET PQL EN PEVTNS+ EDMELESTEP    K + GDS+ANEAAPVTNGDA+SKRE
Sbjct: 1   MAEETIPQLGENGPEVTNSSAEDMELESTEP----KVSGGDSDANEAAPVTNGDANSKRE 60

Query: 61  REEESTDDSVEEAKKQKVEKSVEEERLEKAEGDGKAEET-GPVSLGPKSFGSSVELFDYF 120
           REEE   D V EAKK KVEKSVEEERLEK E DGK EE  GPVSLGPKSFGSSVELFDYF
Sbjct: 61  REEEGAGDCVGEAKKLKVEKSVEEERLEKVERDGKGEEEHGPVSLGPKSFGSSVELFDYF 120

Query: 121 YSFLHHWPANLNVNQDNFAVQRGKLWLVFGNPCILGRLQYEKMVLLDLIKKGHVEPEKKI 180
           Y+FLH+WPANLNVN                        QYE MVLLDLIKKGHVEP+KKI
Sbjct: 121 YNFLHYWPANLNVN------------------------QYELMVLLDLIKKGHVEPDKKI 180

Query: 181 GCGINAFQIRYHPMWKSKCFFLIREDESSDDFSFRKCVDHILPLPENLKAKSDANRALGG 240
           GCG+ A QIRYHPMWKSKCFFLIRED S+DDFSFRKCVDHILPLPENLK  SDANRALGG
Sbjct: 181 GCGVRACQIRYHPMWKSKCFFLIREDGSADDFSFRKCVDHILPLPENLKTISDANRALGG 228

Query: 241 GKHRGGGRGGGRGRGGRWRN 260
           GKHRGGGRGG    GGRWRN
Sbjct: 241 GKHRGGGRGG----GGRWRN 228

[Close]

BLAST of Spg022644 vs. NCBI nr
Match: XP_022972943.1 (protein EMBRYO DEFECTIVE 514-like [Cucurbita maxima])

HSP 1 Score: 344.7 bits (883), Expect = 6.7e-91
Identity = 196/260 (75.38%), Postives = 205/260 (78.85%), Query Frame = 0


Query: 1   MAEETTPQLPENDPEVTNSATEDMELESTEPGAETKANNGDSEANEAAPVTNGDASSKRE 60
           MAEET PQL EN PEVTNSA EDMELESTEP    K + GDS+ANEAAPVTNGDA+SKRE
Sbjct: 1   MAEETIPQLAENGPEVTNSAAEDMELESTEP----KVSGGDSDANEAAPVTNGDANSKRE 60

Query: 61  REEESTDDSVEEAKKQKVEKSVEEERLEKAEGDGKAEET-GPVSLGPKSFGSSVELFDYF 120
           REEE   D V EAKK KVEKSVEEERLEK E DGK EE  GPVSLGPKSFGSSVELF+YF
Sbjct: 61  REEEGAGDYVGEAKKPKVEKSVEEERLEKVERDGKGEEEHGPVSLGPKSFGSSVELFEYF 120

Query: 121 YSFLHHWPANLNVNQDNFAVQRGKLWLVFGNPCILGRLQYEKMVLLDLIKKGHVEPEKKI 180
           Y+FLH+WPANLNVN                        QYE MVLLDLIKKGHVEP+KKI
Sbjct: 121 YNFLHYWPANLNVN------------------------QYELMVLLDLIKKGHVEPDKKI 180

Query: 181 GCGINAFQIRYHPMWKSKCFFLIREDESSDDFSFRKCVDHILPLPENLKAKSDANRALGG 240
           GCG+ A QIRYHPMWKSKCFFLIRED S+DDFSFRKCVDHILPLPENLK  SDANRALGG
Sbjct: 181 GCGVRACQIRYHPMWKSKCFFLIREDGSADDFSFRKCVDHILPLPENLKTISDANRALGG 228

Query: 241 GKHRGGGRGGGRGRGGRWRN 260
           GKHRGGGRGG    GGRWRN
Sbjct: 241 GKHRGGGRGG----GGRWRN 228

[Close]

BLAST of Spg022644 vs. ExPASy Swiss-Prot
Match: Q8L557 (Protein EMBRYO DEFECTIVE 514 OS=Arabidopsis thaliana OX=3702 GN=EMB514 PE=1 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 1.8e-46
Identity = 120/246 (48.78%), Postives = 143/246 (58.13%), Query Frame = 0


Query: 15  EVTNSATEDMELESTEPGAETKANNGDSEANEAAPVTNGDASSKREREEESTDDSVEEAK 74
           EV      DME+E+  P AET                 GD   KREREE   +++  E+K
Sbjct: 14  EVNPDQKVDMEVETATPKAET-----------------GD--EKREREETEEEENGGESK 73

Query: 75  KQKVEKSVEEERLEKAEGDGKAEETGPVSLGPKSFGSSVELFDYFYSFLHHWPANLNVNQ 134
           KQKV               G+ E++GPV LGPK F +SV +FDYF  FLH WP +L+VN 
Sbjct: 74  KQKV---------------GEEEKSGPVKLGPKEFVTSVAMFDYFVKFLHFWPTDLDVN- 133

Query: 135 DNFAVQRGKLWLVFGNPCILGRLQYEKMVLLDLIKKGHVEPEKKIGCGINAFQIRYHPMW 194
                                  +YE MVLLDLIKKGH EPEKKIG GI  FQ+R HPMW
Sbjct: 134 -----------------------KYEHMVLLDLIKKGHSEPEKKIGGGIKTFQVRTHPMW 193

Query: 195 KSKCFFLIREDESSDDFSFRKCVDHILPLPENLKAKSDANRALGGGKHRGGGRGGGR--G 254
           KS+CFFL+RED+++DDFSFRKCVD ILPLPEN+K         GGG+  GGGR GGR  G
Sbjct: 194 KSRCFFLVREDDTADDFSFRKCVDQILPLPENMKTPGANGNGHGGGRGGGGGRRGGRGGG 201

Query: 255 RGGRWR 259
           RGGR+R
Sbjct: 254 RGGRFR 201

[Close]

BLAST of Spg022644 vs. ExPASy Swiss-Prot
Match: Q9C642 (Protein DCL homolog, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DCL PE=2 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 8.2e-07
Identity = 25/69 (36.23%), Postives = 43/69 (62.32%), Query Frame = 0


Query: 164 LLDLIKKGHVEPEKKIGCGINAFQIRYHPMWK-SKCFFLIREDESSDDFSFRKCVDHILP 223
           +++++   H E EKKIGCGI+   + +HP ++ S+C F++R+D    DFS+ KC+  ++ 
Sbjct: 138 IIEMLLPYHPECEKKIGCGIDYIMVGHHPDFESSRCMFIVRKDGEVVDFSYWKCIKGLIK 197

Query: 224 LPENLKAKS 232
               L A S
Sbjct: 198 KKYPLYADS 206

[Close]

BLAST of Spg022644 vs. ExPASy Swiss-Prot
Match: Q42463 (Protein DCL, chloroplastic OS=Solanum lycopersicum OX=4081 GN=DCL PE=2 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 5.9e-05
Identity = 20/51 (39.22%), Postives = 35/51 (68.63%), Query Frame = 0


Query: 172 HVEPEKKIGCGINAFQIRYHPMWK-SKCFFLIREDESSDDFSFRKCVDHIL 222
           H E +KKIG G++   + YHP ++ S+C F++R+D  + DFS+ KC+  ++
Sbjct: 151 HPECDKKIGPGVDYITVGYHPDFENSRCLFIVRKDGETVDFSYWKCIKGLI 201

[Close]

BLAST of Spg022644 vs. ExPASy TrEMBL
Match: A0A6J1C0K4 (protein EMBRYO DEFECTIVE 514 OS=Momordica charantia OX=3673 GN=LOC111007244 PE=4 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 1.1e-94
Identity = 198/262 (75.57%), Postives = 218/262 (83.21%), Query Frame = 0


Query: 1   MAEETTPQLPENDPEVTNSATEDMELESTEPGAETKANNGDSEANEAAPVTNGDASSKRE 60
           MAEETTPQL ENDPEVTNSATEDMELE++EPGAE K  NGDSEANEAAP TNGDA+SKRE
Sbjct: 1   MAEETTPQLAENDPEVTNSATEDMELETSEPGAEPKGLNGDSEANEAAPETNGDANSKRE 60

Query: 61  REEESTDDSVEEAKKQKVEKSVEEERLEKAEGDGKAEE-TGPVSLGPKSFGSSVELFDYF 120
           REEE+ D+++ EAKK KVEKSVEEER+EK EGDGK +E +GPVSLGPKSFGSSVE+FDYF
Sbjct: 61  REEETADENIGEAKKPKVEKSVEEERMEKVEGDGKGDEKSGPVSLGPKSFGSSVEMFDYF 120

Query: 121 YSFLHHWPANLNVNQDNFAVQRGKLWLVFGNPCILGRLQYEKMVLLDLIKKGHVEPEKKI 180
           Y FLH+WPANLN+N                        +YE+MVLL+L+KKGHVEPEKKI
Sbjct: 121 YKFLHYWPANLNLN------------------------EYEQMVLLELLKKGHVEPEKKI 180

Query: 181 GCGINAFQIRYHPMWKSKCFFLIREDESSDDFSFRKCVDHILPLPENLKAKSDANRALGG 240
           GCGI+AFQIR HP +KSKC+FLIREDES DDFSFRKCVDHILPLPENLK KSDANRALGG
Sbjct: 181 GCGIHAFQIRNHPTFKSKCYFLIREDESVDDFSFRKCVDHILPLPENLKVKSDANRALGG 238

Query: 241 GKHRGGGR--GGGRGRGGRWRN 260
            KHRGGGR  GGGRGRGGR RN
Sbjct: 241 CKHRGGGRGGGGGRGRGGRSRN 238

[Close]

BLAST of Spg022644 vs. ExPASy TrEMBL
Match: A0A6J1F569 (protein EMBRYO DEFECTIVE 514-like OS=Cucurbita moschata OX=3662 GN=LOC111440949 PE=4 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 3.8e-92
Identity = 197/260 (75.77%), Postives = 205/260 (78.85%), Query Frame = 0


Query: 1   MAEETTPQLPENDPEVTNSATEDMELESTEPGAETKANNGDSEANEAAPVTNGDASSKRE 60
           MAEET PQL EN PEVTNSA EDMELESTEP    K + GDS+ANEAAPVTNGDA+SKRE
Sbjct: 1   MAEETIPQLAENGPEVTNSAAEDMELESTEP----KVSGGDSDANEAAPVTNGDANSKRE 60

Query: 61  REEESTDDSVEEAKKQKVEKSVEEERLEKAEGDGKAEET-GPVSLGPKSFGSSVELFDYF 120
           REEE  DD   EAKK KVEKSVEEERLEK E DGK EE  GPVSLGPKSFGSSVELFDYF
Sbjct: 61  REEEGADDHAGEAKKPKVEKSVEEERLEKVERDGKGEEEHGPVSLGPKSFGSSVELFDYF 120

Query: 121 YSFLHHWPANLNVNQDNFAVQRGKLWLVFGNPCILGRLQYEKMVLLDLIKKGHVEPEKKI 180
           Y+FLH+WPANLNVN                        QYE MVLLDLIKKGHVEP+KKI
Sbjct: 121 YNFLHYWPANLNVN------------------------QYELMVLLDLIKKGHVEPDKKI 180

Query: 181 GCGINAFQIRYHPMWKSKCFFLIREDESSDDFSFRKCVDHILPLPENLKAKSDANRALGG 240
           GCG+ A QIRYHPMWKSKCFFLIRED S+DDFSFRKCVDHILPLPENLK  SDANRALGG
Sbjct: 181 GCGVRACQIRYHPMWKSKCFFLIREDGSADDFSFRKCVDHILPLPENLKTISDANRALGG 228

Query: 241 GKHRGGGRGGGRGRGGRWRN 260
           GKHRGGGRGG    GGRWRN
Sbjct: 241 GKHRGGGRGG----GGRWRN 228

[Close]

BLAST of Spg022644 vs. ExPASy TrEMBL
Match: A0A6J1IBL1 (protein EMBRYO DEFECTIVE 514-like OS=Cucurbita maxima OX=3661 GN=LOC111471455 PE=4 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 3.3e-91
Identity = 196/260 (75.38%), Postives = 205/260 (78.85%), Query Frame = 0


Query: 1   MAEETTPQLPENDPEVTNSATEDMELESTEPGAETKANNGDSEANEAAPVTNGDASSKRE 60
           MAEET PQL EN PEVTNSA EDMELESTEP    K + GDS+ANEAAPVTNGDA+SKRE
Sbjct: 1   MAEETIPQLAENGPEVTNSAAEDMELESTEP----KVSGGDSDANEAAPVTNGDANSKRE 60

Query: 61  REEESTDDSVEEAKKQKVEKSVEEERLEKAEGDGKAEET-GPVSLGPKSFGSSVELFDYF 120
           REEE   D V EAKK KVEKSVEEERLEK E DGK EE  GPVSLGPKSFGSSVELF+YF
Sbjct: 61  REEEGAGDYVGEAKKPKVEKSVEEERLEKVERDGKGEEEHGPVSLGPKSFGSSVELFEYF 120

Query: 121 YSFLHHWPANLNVNQDNFAVQRGKLWLVFGNPCILGRLQYEKMVLLDLIKKGHVEPEKKI 180
           Y+FLH+WPANLNVN                        QYE MVLLDLIKKGHVEP+KKI
Sbjct: 121 YNFLHYWPANLNVN------------------------QYELMVLLDLIKKGHVEPDKKI 180

Query: 181 GCGINAFQIRYHPMWKSKCFFLIREDESSDDFSFRKCVDHILPLPENLKAKSDANRALGG 240
           GCG+ A QIRYHPMWKSKCFFLIRED S+DDFSFRKCVDHILPLPENLK  SDANRALGG
Sbjct: 181 GCGVRACQIRYHPMWKSKCFFLIREDGSADDFSFRKCVDHILPLPENLKTISDANRALGG 228

Query: 241 GKHRGGGRGGGRGRGGRWRN 260
           GKHRGGGRGG    GGRWRN
Sbjct: 241 GKHRGGGRGG----GGRWRN 228

[Close]

BLAST of Spg022644 vs. ExPASy TrEMBL
Match: A0A0A0LRN9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G009730 PE=4 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 1.9e-86
Identity = 191/264 (72.35%), Postives = 203/264 (76.89%), Query Frame = 0


Query: 1   MAEETTPQLPENDPEVTNSATEDMELESTEPGAETKANNGDSEANEAAPVTNGDASSKRE 60
           MAEET PQL   DPEVTNS TEDMELE+TEP A T A    SEANEAAPV NGDA+SKRE
Sbjct: 1   MAEETIPQL---DPEVTNSGTEDMELETTEPKASTDA----SEANEAAPVINGDATSKRE 60

Query: 61  REEESTDDSVEEAKKQKVEKSVEEERLEKAEGDGKAEETG-PVSLGPKSFGSSVELFDYF 120
           R EES DD V + KKQK+EKSVEEERLEK  GDGK EE   PVSLGPKSF SSVELFDYF
Sbjct: 61  R-EESADDGVADGKKQKIEKSVEEERLEKLGGDGKCEEEPVPVSLGPKSFRSSVELFDYF 120

Query: 121 YSFLHHWPANLNVNQDNFAVQRGKLWLVFGNPCILGRLQYEKMVLLDLIKKGHVEPEKKI 180
           Y  LHHWP NLNVN                        QYE+MVL+DL+KKGH+EPEKKI
Sbjct: 121 YKILHHWPVNLNVN------------------------QYEQMVLVDLLKKGHLEPEKKI 180

Query: 181 GCGINAFQIRYHPMWKSKCFFLIREDESSDDFSFRKCVDHILPLPENLKAKSDANRALGG 240
           GCGI++FQIR+HP WKSKCFFLIREDES+DDFSFRKCVDHILPLPEN K K DANRALGG
Sbjct: 181 GCGIHSFQIRFHPEWKSKCFFLIREDESADDFSFRKCVDHILPLPENFKGKFDANRALGG 232

Query: 241 GKHRGGGRGG----GRGRGGRWRN 260
           GKHRGGGRGG    GRG GGRWRN
Sbjct: 241 GKHRGGGRGGGGGRGRGGGGRWRN 232

[Close]

BLAST of Spg022644 vs. ExPASy TrEMBL
Match: A0A6J1JDH2 (protein EMBRYO DEFECTIVE 514-like OS=Cucurbita maxima OX=3661 GN=LOC111484822 PE=4 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 1.6e-85
Identity = 187/271 (69.00%), Postives = 208/271 (76.75%), Query Frame = 0


Query: 1   MAEETTPQLPENDPEVTNSATEDMELESTEPGAETKANNGDSEANEAAPVTNGDASSKRE 60
           MAEET PQ  +N+ EVTN+  EDMELE+TEPGAE K +N   EANEA+ +TNGDA+SKRE
Sbjct: 1   MAEETIPQPADNNQEVTNA--EDMELENTEPGAEPKGSN--DEANEASKITNGDANSKRE 60

Query: 61  REEESTDDSVEEAKKQKVEKSVEEERLEKAEGDGKA-EETGPVSLGPKSFGSSVELFDYF 120
           REEES D SVEEAKKQK+EKSVEE R+EKA+GDGK  EE GPV++GPKSF SS ELFD+F
Sbjct: 61  REEESADGSVEEAKKQKMEKSVEEGRVEKAQGDGKGEEEPGPVTIGPKSFRSSRELFDHF 120

Query: 121 YSFLHHWPANLNVNQDNFAVQRGKLWLVFGNPCILGRLQYEKMVLLDLIKKGHVEPEKKI 180
           Y FLHHWPA LNVN                        QYEKM+LLDL+KKGH EPEKKI
Sbjct: 121 YKFLHHWPAYLNVN------------------------QYEKMMLLDLLKKGHAEPEKKI 180

Query: 181 GCGINAFQIRYHPMWKSKCFFLIREDESSDDFSFRKCVDHILPLPENLKAKSDANRALGG 240
           GCGI++FQIR HPMWK+KCFFLIREDES DDFSFRKCVDHILPLPENLK +SDAN+A GG
Sbjct: 181 GCGIHSFQIRNHPMWKNKCFFLIREDESVDDFSFRKCVDHILPLPENLKGRSDANKAFGG 240

Query: 241 GKHRG-----------GGRGGGRGRGGRWRN 260
           GKHRG           GG GGGRGRGGRWRN
Sbjct: 241 GKHRGGGGGGGSGGGRGGGGGGRGRGGRWRN 243

[Close]

BLAST of Spg022644 vs. TAIR 10
Match: AT5G62440.1 (Protein of unknown function (DUF3223) )

HSP 1 Score: 187.6 bits (475), Expect = 1.3e-47
Identity = 120/246 (48.78%), Postives = 143/246 (58.13%), Query Frame = 0


Query: 15  EVTNSATEDMELESTEPGAETKANNGDSEANEAAPVTNGDASSKREREEESTDDSVEEAK 74
           EV      DME+E+  P AET                 GD   KREREE   +++  E+K
Sbjct: 14  EVNPDQKVDMEVETATPKAET-----------------GD--EKREREETEEEENGGESK 73

Query: 75  KQKVEKSVEEERLEKAEGDGKAEETGPVSLGPKSFGSSVELFDYFYSFLHHWPANLNVNQ 134
           KQKV               G+ E++GPV LGPK F +SV +FDYF  FLH WP +L+VN 
Sbjct: 74  KQKV---------------GEEEKSGPVKLGPKEFVTSVAMFDYFVKFLHFWPTDLDVN- 133

Query: 135 DNFAVQRGKLWLVFGNPCILGRLQYEKMVLLDLIKKGHVEPEKKIGCGINAFQIRYHPMW 194
                                  +YE MVLLDLIKKGH EPEKKIG GI  FQ+R HPMW
Sbjct: 134 -----------------------KYEHMVLLDLIKKGHSEPEKKIGGGIKTFQVRTHPMW 193

Query: 195 KSKCFFLIREDESSDDFSFRKCVDHILPLPENLKAKSDANRALGGGKHRGGGRGGGR--G 254
           KS+CFFL+RED+++DDFSFRKCVD ILPLPEN+K         GGG+  GGGR GGR  G
Sbjct: 194 KSRCFFLVREDDTADDFSFRKCVDQILPLPENMKTPGANGNGHGGGRGGGGGRRGGRGGG 201

Query: 255 RGGRWR 259
           RGGR+R
Sbjct: 254 RGGRFR 201

[Close]

BLAST of Spg022644 vs. TAIR 10
Match: AT1G45230.1 (Protein of unknown function (DUF3223) )

HSP 1 Score: 55.8 bits (133), Expect = 5.8e-08
Identity = 25/69 (36.23%), Postives = 43/69 (62.32%), Query Frame = 0


Query: 164 LLDLIKKGHVEPEKKIGCGINAFQIRYHPMWK-SKCFFLIREDESSDDFSFRKCVDHILP 223
           +++++   H E EKKIGCGI+   + +HP ++ S+C F++R+D    DFS+ KC+  ++ 
Sbjct: 138 IIEMLLPYHPECEKKIGCGIDYIMVGHHPDFESSRCMFIVRKDGEVVDFSYWKCIKGLIK 197

Query: 224 LPENLKAKS 232
               L A S
Sbjct: 198 KKYPLYADS 206

[Close]

BLAST of Spg022644 vs. TAIR 10
Match: AT1G45230.2 (Protein of unknown function (DUF3223) )

HSP 1 Score: 55.5 bits (132), Expect = 7.6e-08
Identity = 25/69 (36.23%), Postives = 43/69 (62.32%), Query Frame = 0


Query: 164 LLDLIKKGHVEPEKKIGCGINAFQIRYHPMWK-SKCFFLIREDESSDDFSFRKCVDHILP 223
           +++++   H E EKKIGCGI+   + +HP ++ S+C F++R+D    DFS+ KC+  ++ 
Sbjct: 138 IIEMLLPYHPECEKKIGCGIDYIMVWHHPDFESSRCMFIVRKDGEVVDFSYWKCIKGLIK 197

Query: 224 LPENLKAKS 232
               L A S
Sbjct: 198 KKYPLYADS 206

[Close]

BLAST of Spg022644 vs. TAIR 10
Match: AT2G40030.1 (nuclear RNA polymerase D1B )

HSP 1 Score: 46.2 bits (108), Expect = 4.6e-05
Identity = 20/63 (31.75%), Postives = 38/63 (60.32%), Query Frame = 0


Query: 160  EKMVLLDLIKKGHVEPEKKIGCGINAFQIRYHPMWK-SKCFFLIREDESSDDFSFRKCVD 219
            +K  +L+ I   H + E K+G G++   +  H ++  S+CFF++  D +  DFS+RK ++
Sbjct: 1769 DKTFVLEKILNFHPQKETKLGSGVDFITVDKHTIFSDSRCFFVVSTDGAKQDFSYRKSLN 1828

Query: 220  HIL 222
            + L
Sbjct: 1829 NYL 1831

The following BLAST results are available for this feature:

BLAST of Spg022644 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022135230.1	2.2e-94	75.57	protein EMBRYO DEFECTIVE 514 [Momordica charantia]	[more]
XP_022933555.1	7.9e-92	75.77	protein EMBRYO DEFECTIVE 514-like [Cucurbita moschata]	[more]
KAG7021514.1	1.0e-91	75.77	Protein EMBRYO DEFECTIVE [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023529405.1	6.7e-91	75.38	protein EMBRYO DEFECTIVE 514-like [Cucurbita pepo subsp. pepo]	[more]
XP_022972943.1	6.7e-91	75.38	protein EMBRYO DEFECTIVE 514-like [Cucurbita maxima]	[more]

BLAST of Spg022644 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L557	1.8e-46	48.78	Protein EMBRYO DEFECTIVE 514 OS=Arabidopsis thaliana OX=3702 GN=EMB514 PE=1 SV=1	[more]
Q9C642	8.2e-07	36.23	Protein DCL homolog, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DCL PE=2 S...	[more]
Q42463	5.9e-05	39.22	Protein DCL, chloroplastic OS=Solanum lycopersicum OX=4081 GN=DCL PE=2 SV=1	[more]

BLAST of Spg022644 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C0K4	1.1e-94	75.57	protein EMBRYO DEFECTIVE 514 OS=Momordica charantia OX=3673 GN=LOC111007244 PE=4...	[more]
A0A6J1F569	3.8e-92	75.77	protein EMBRYO DEFECTIVE 514-like OS=Cucurbita moschata OX=3662 GN=LOC111440949 ...	[more]
A0A6J1IBL1	3.3e-91	75.38	protein EMBRYO DEFECTIVE 514-like OS=Cucurbita maxima OX=3661 GN=LOC111471455 PE...	[more]
A0A0A0LRN9	1.9e-86	72.35	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G009730 PE=4 SV=1	[more]
A0A6J1JDH2	1.6e-85	69.00	protein EMBRYO DEFECTIVE 514-like OS=Cucurbita maxima OX=3661 GN=LOC111484822 PE...	[more]

BLAST of Spg022644 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G62440.1	1.3e-47	48.78	Protein of unknown function (DUF3223)	[more]
AT1G45230.1	5.8e-08	36.23	Protein of unknown function (DUF3223)	[more]
AT1G45230.2	7.6e-08	36.23	Protein of unknown function (DUF3223)	[more]
AT2G40030.1	4.6e-05	31.75	nuclear RNA polymerase D1B	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 70..93
None	No IPR available	PFAM	PF11523	DUF3223	coord: 159..217 e-value: 1.1E-20 score: 74.1
None	No IPR available	GENE3D	3.10.450.40		coord: 156..220 e-value: 2.6E-17 score: 65.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 10..25
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..103
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 232..259
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 32..52
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 55..96
None	No IPR available	PANTHER	PTHR33415:SF12	PROTEIN EMBRYO DEFECTIVE 514	coord: 158..251 coord: 15..139
IPR044673	Protein DCL-like	PANTHER	PTHR33415	FAMILY NOT NAMED	coord: 158..251 coord: 15..139

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg022644.1	Spg022644.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0017126	nucleologenesis
cellular_component	GO:0005634	nucleus