Spg022635 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg022635
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	L-ascorbate oxidase
Location	scaffold2: 11106364 .. 11108158 (+)
RNA-Seq Expression	Spg022635
Synteny	Spg022635

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACCCCAGAGGCGAAACCGACAAATGGGACGGGTCAAGACCAAAGGGGTCGGGCATTTGGCCCAACCCCCCTGCTCGGCTTGCGCGGGCGGTGTCCCACCGACTCCGTTCGGTCCGTGCAGCCTCTAGCCACCCCGGTTTCGCCTGGTTTGTCCCGAAACGCCTCCGAATTCCTAAAAACCCTAGGAGCATGAGCAGGTATTTATACCACTCTTCGCCACTAAAGAAGAGACCCGAACTCTCGACTCTCTCCTCTTACTCTCTAGCTCTCCTCTTCCGATTTCTGACTTAAGCATTGGAGGCGGTGTGGCTAGCACCACACCGGTGTGCAGGTTTTCCGTTTTGCAGGTCACGTCTTCCCCCTCATCTACAAATTTACTGTTGGTGACACGTGAAGGTCAGGTGAGTTTCCTCTGACCAAATTTGACCATCAACAGTTACAATTATACTCATTAATTTGTCATCGTAAAAATCATATTCTCACGATTACATAATTGGATGCCAACTTGAACATATTTTTATTAGTTAAGACTTATATCAATCAACCAAAAGAGCACGATAAAATATTCTCCACCCCGTATATTGTTCAACCTAAAAAACAAATTTACATATATAATTCTTTAAACATATACTCCTACAAGTCACCTAAATTACTTCTCTTGATTTCACAAATAATATGCTGATGAAGGACTGCCAACTTACTTAAAGCGTAACACAACAAAGTGAGTCTAAATCAACGGTAACTGACATGTATTTTCAACTTAGAGATCAATCCTAATAGGCGTGTCGAATATCTAGATTCTTCAACTTTTAATTTTTATTTCTTATTTCTTGTTTTTCGATAGAATCACGAGATGACAGTAGTAGAAGCCGATGCGAACTACGTCCAACCATTTATAACTTCCAGCATCGACGTTCAGTCCGGCGAGACCTACTCCGTCCTCATAACCACCGATCAAGACCCATCCATCAACTACTGGCTCTCCGCCGCCGTCACCGGACACAATCTCGCCACCCCTCCGGCGCTCACCCTCCTCCACTACATCCCCACTCCGGCCACCAAGCTCCCCCCTTCTCCGCCACCCCCTCCTCCGCAGTGGAACGATTTCGACCGCCTCAAGCAATTCGCCAACCAACTCCTCGCCGCCGCTGGCTCCCCACAACCGCCGCCCAAGTACAACCGTCGAATGTCGTTCCTCACCACCCAGAACGAGAACGACGGAGTCCTGAAGTGGGCCATCAACAACGTCTCCTTATCCCTCCCAACGACGCCGTATTTGGGCGCCATCAAATTCGGCCTGAACCGATCCTTCGACCAGTTTACCCCGCCGGAGACTTTCCCGGAGAACTACGACATCGACATCCCTCTGATCAACAACGCCACCGCAGTCAGCAGCGCGATTTATCGACTCGAGTTTGGGTCGACGGTGGACATCGTGCTTCAAAATGCGAACACGTTGGTGAAAAATGTCGGCGAAATTCATCCCTGGCATTTGCACGGACATGATTTCTGGGTTTTGGGTTTTGGCCCTTTTGGGGAAGGGAAATTTTCGGCGGCCGATGAGAAGCTGCTGAATTTGAAGAACCCGGCGCTGAAGAACACGGTGGTGGTTTTTCCATATGGGTGGACGGCGGTGAGGTTTGTGGCGGATAATCCTGGAGTTTGGATATTCCATTGCCATATCGAACCTCATCTGCATATGGGAATGGGCGTTGTTATTGCTGAAGGAGTTGATAGAGTTGGTGATATTCCTAAAAATGCTCTCTCTTGTGGCCTCACTGGACAAATGTAG

mRNA sequence

ATGGACCCCAGAGGCGAAACCGACAAATGGGACGGGTCAAGACCAAAGGGGTCGGGCATTTGGCCCAACCCCCCTGCTCGGCTTGCGCGGGCGGTGTCCCACCGACTCCGTTCGGTCCGTGCAGCCTCTAGCCACCCCGGTTTCGCCTGGTTTGTCCCGAAACGCCTCCGAATTCCTAAAAACCCTAGGAGCATGAGCAGCATTGGAGGCGGTGTGGCTAGCACCACACCGGTGTGCAGGTTTTCCGTTTTGCAGGTCACGTCTTCCCCCTCATCTACAAATTTACTGTTGGTGACACGTGAAGGTCAGAATCACGAGATGACAGTAGTAGAAGCCGATGCGAACTACGTCCAACCATTTATAACTTCCAGCATCGACGTTCAGTCCGGCGAGACCTACTCCGTCCTCATAACCACCGATCAAGACCCATCCATCAACTACTGGCTCTCCGCCGCCGTCACCGGACACAATCTCGCCACCCCTCCGGCGCTCACCCTCCTCCACTACATCCCCACTCCGGCCACCAAGCTCCCCCCTTCTCCGCCACCCCCTCCTCCGCAGTGGAACGATTTCGACCGCCTCAAGCAATTCGCCAACCAACTCCTCGCCGCCGCTGGCTCCCCACAACCGCCGCCCAAGTACAACCGTCGAATGTCGTTCCTCACCACCCAGAACGAGAACGACGGAGTCCTGAAGTGGGCCATCAACAACGTCTCCTTATCCCTCCCAACGACGCCGTATTTGGGCGCCATCAAATTCGGCCTGAACCGATCCTTCGACCAGTTTACCCCGCCGGAGACTTTCCCGGAGAACTACGACATCGACATCCCTCTGATCAACAACGCCACCGCAGTCAGCAGCGCGATTTATCGACTCGAGTTTGGGTCGACGGTGGACATCGTGCTTCAAAATGCGAACACGTTGGTGAAAAATGTCGGCGAAATTCATCCCTGGCATTTGCACGGACATGATTTCTGGGTTTTGGGTTTTGGCCCTTTTGGGGAAGGGAAATTTTCGGCGGCCGATGAGAAGCTGCTGAATTTGAAGAACCCGGCGCTGAAGAACACGGTGGTGGTTTTTCCATATGGGTGGACGGCGGTGAGGTTTGTGGCGGATAATCCTGGAGTTTGGATATTCCATTGCCATATCGAACCTCATCTGCATATGGGAATGGGCGTTGTTATTGCTGAAGGAGTTGATAGAGTTGGTGATATTCCTAAAAATGCTCTCTCTTGTGGCCTCACTGGACAAATGTAG

Coding sequence (CDS)

ATGGACCCCAGAGGCGAAACCGACAAATGGGACGGGTCAAGACCAAAGGGGTCGGGCATTTGGCCCAACCCCCCTGCTCGGCTTGCGCGGGCGGTGTCCCACCGACTCCGTTCGGTCCGTGCAGCCTCTAGCCACCCCGGTTTCGCCTGGTTTGTCCCGAAACGCCTCCGAATTCCTAAAAACCCTAGGAGCATGAGCAGCATTGGAGGCGGTGTGGCTAGCACCACACCGGTGTGCAGGTTTTCCGTTTTGCAGGTCACGTCTTCCCCCTCATCTACAAATTTACTGTTGGTGACACGTGAAGGTCAGAATCACGAGATGACAGTAGTAGAAGCCGATGCGAACTACGTCCAACCATTTATAACTTCCAGCATCGACGTTCAGTCCGGCGAGACCTACTCCGTCCTCATAACCACCGATCAAGACCCATCCATCAACTACTGGCTCTCCGCCGCCGTCACCGGACACAATCTCGCCACCCCTCCGGCGCTCACCCTCCTCCACTACATCCCCACTCCGGCCACCAAGCTCCCCCCTTCTCCGCCACCCCCTCCTCCGCAGTGGAACGATTTCGACCGCCTCAAGCAATTCGCCAACCAACTCCTCGCCGCCGCTGGCTCCCCACAACCGCCGCCCAAGTACAACCGTCGAATGTCGTTCCTCACCACCCAGAACGAGAACGACGGAGTCCTGAAGTGGGCCATCAACAACGTCTCCTTATCCCTCCCAACGACGCCGTATTTGGGCGCCATCAAATTCGGCCTGAACCGATCCTTCGACCAGTTTACCCCGCCGGAGACTTTCCCGGAGAACTACGACATCGACATCCCTCTGATCAACAACGCCACCGCAGTCAGCAGCGCGATTTATCGACTCGAGTTTGGGTCGACGGTGGACATCGTGCTTCAAAATGCGAACACGTTGGTGAAAAATGTCGGCGAAATTCATCCCTGGCATTTGCACGGACATGATTTCTGGGTTTTGGGTTTTGGCCCTTTTGGGGAAGGGAAATTTTCGGCGGCCGATGAGAAGCTGCTGAATTTGAAGAACCCGGCGCTGAAGAACACGGTGGTGGTTTTTCCATATGGGTGGACGGCGGTGAGGTTTGTGGCGGATAATCCTGGAGTTTGGATATTCCATTGCCATATCGAACCTCATCTGCATATGGGAATGGGCGTTGTTATTGCTGAAGGAGTTGATAGAGTTGGTGATATTCCTAAAAATGCTCTCTCTTGTGGCCTCACTGGACAAATGTAG

Protein sequence

MDPRGETDKWDGSRPKGSGIWPNPPARLARAVSHRLRSVRAASSHPGFAWFVPKRLRIPKNPRSMSSIGGGVASTTPVCRFSVLQVTSSPSSTNLLLVTREGQNHEMTVVEADANYVQPFITSSIDVQSGETYSVLITTDQDPSINYWLSAAVTGHNLATPPALTLLHYIPTPATKLPPSPPPPPPQWNDFDRLKQFANQLLAAAGSPQPPPKYNRRMSFLTTQNENDGVLKWAINNVSLSLPTTPYLGAIKFGLNRSFDQFTPPETFPENYDIDIPLINNATAVSSAIYRLEFGSTVDIVLQNANTLVKNVGEIHPWHLHGHDFWVLGFGPFGEGKFSAADEKLLNLKNPALKNTVVVFPYGWTAVRFVADNPGVWIFHCHIEPHLHMGMGVVIAEGVDRVGDIPKNALSCGLTGQM

Homology

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BLAST of Spg022635 vs. NCBI nr
Match: KAE8037705.1 (hypothetical protein FH972_010271 [Carpinus fangiana])

HSP 1 Score: 446.0 bits (1146), Expect = 3.5e-121
Identity = 206/328 (62.80%), Postives = 251/328 (76.52%), Query Frame = 0


Query: 91  SSTNLLLVTREGQNHEMTVVEADANYVQPFITSSIDVQSGETYSVLITTDQDPSINYWLS 150
           S+T L  +     NH+M VVEAD NY+QPF    +D+ SGE+YSVLITT+QDPS NYW+S
Sbjct: 238 STTALASLNLAIGNHKMVVVEADGNYLQPFAVDDLDIYSGESYSVLITTNQDPSKNYWIS 297

Query: 151 AAVTGHNLATPPALTLLHYIPTPATKLPPSPPPPPPQWNDFDRLKQFANQLLAAAGSPQP 210
             V G + ATP  LT+L+Y PT A+KLP SPPP  P+WND+   K F+N++LA  GSP+P
Sbjct: 298 VGVRGRSPATPQGLTILNYKPTSASKLPSSPPPVTPRWNDYVHSKTFSNKILARMGSPRP 357

Query: 211 PPKYNRRMSFLTTQNENDGVLKWAINNVSLSLPTTPYLGAIKFGLNRSFDQFTPPETFPE 270
           P KY+RR+  L TQN+ +G  KWA+NNVSL+LP TPYLG+IK+GL  +FDQ TPPE F +
Sbjct: 358 PTKYDRRIFLLNTQNKINGFTKWALNNVSLTLPATPYLGSIKYGLKNAFDQKTPPENFAD 417

Query: 271 NYDIDIPLINNATAVSSAIYRLEFGSTVDIVLQNANTLVKNVGEIHPWHLHGHDFWVLGF 330
           NYD+ +P IN  T+  S  Y +E  STVD++LQNAN L  NV EIHPWHLHGHDFWVLG 
Sbjct: 418 NYDVMVPPINPNTSTGSGAYIIELNSTVDLILQNANALSDNVSEIHPWHLHGHDFWVLG- 477

Query: 331 GPFGEGKFSAADEKLLNLKNPALKNTVVVFPYGWTAVRFVADNPGVWIFHCHIEPHLHMG 390
             +GEGKFS  DEK LNLKNP L+NT V+FPYGWTA+RFVADNPG W FHCHIEPHLHMG
Sbjct: 478 --YGEGKFSEKDEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGAWAFHCHIEPHLHMG 537

Query: 391 MGVVIAEGVDRVGDIPKNALSCGLTGQM 419
           MGVV AEG+ RV +IP+ A++CGLT +M
Sbjct: 538 MGVVFAEGIHRVDNIPREAIACGLTAKM 562

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BLAST of Spg022635 vs. NCBI nr
Match: KAG6671128.1 (hypothetical protein I3843_Q014000 [Carya illinoinensis])

HSP 1 Score: 443.0 bits (1138), Expect = 2.9e-120
Identity = 202/317 (63.72%), Postives = 243/317 (76.66%), Query Frame = 0


Query: 101 EGQNHEMTVVEADANYVQPFITSSIDVQSGETYSVLITTDQDPSINYWLSAAVTGHNLAT 160
           E QNH+M VVEAD NY+QPF  + +D+ SGE+YSVLITTDQDPS NYWLS  V G   AT
Sbjct: 202 EPQNHKMVVVEADGNYLQPFAVNDLDIYSGESYSVLITTDQDPSKNYWLSIGVRGRLPAT 261

Query: 161 PPALTLLHYIPTPATKLPPSPPPPPPQWNDFDRLKQFANQLLAAAGSPQPPPKYNRRMSF 220
           PP L++L+Y PT  +K P SPPP  P+W+D++  K F+N + A  GSP+PP  Y+RR++ 
Sbjct: 262 PPGLSILNYQPTSVSKFPTSPPPVTPRWDDYNHSKTFSNSIFALMGSPKPPTSYDRRITL 321

Query: 221 LTTQNENDGVLKWAINNVSLSLPTTPYLGAIKFGLNRSFDQFTPPETFPENYDIDIPLIN 280
           L TQN+ DG  KWAINNVSL+LP TPYLG+IK+GL  +FDQ +PPE FP+NYD+  P IN
Sbjct: 322 LNTQNKIDGFTKWAINNVSLALPPTPYLGSIKYGLQNAFDQKSPPENFPDNYDVMRPPIN 381

Query: 281 NATAVSSAIYRLEFGSTVDIVLQNANTLVKNVGEIHPWHLHGHDFWVLGFGPFGEGKFSA 340
             + + S +Y     STVD++LQNAN+L  NV EIHPWHLHGHDFWVLG   +GEGKFSA
Sbjct: 382 PNSTIGSGVYMFGLNSTVDVILQNANSLSNNVSEIHPWHLHGHDFWVLG---YGEGKFSA 441

Query: 341 ADEKLLNLKNPALKNTVVVFPYGWTAVRFVADNPGVWIFHCHIEPHLHMGMGVVIAEGVD 400
            DEK LN KNP L+NT V+FPYGWTA+R+VADNPGVW FHCHIEPHLHMGMGVV AEGV 
Sbjct: 442 KDEKKLNFKNPPLRNTAVIFPYGWTALRYVADNPGVWAFHCHIEPHLHMGMGVVFAEGVH 501

Query: 401 RVGDIPKNALSCGLTGQ 418
            V  IP  AL+CGLT +
Sbjct: 502 HVKSIPNEALTCGLTAK 515

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BLAST of Spg022635 vs. NCBI nr
Match: KAG6671127.1 (hypothetical protein I3843_Q014000 [Carya illinoinensis])

HSP 1 Score: 443.0 bits (1138), Expect = 2.9e-120
Identity = 202/317 (63.72%), Postives = 243/317 (76.66%), Query Frame = 0


Query: 101 EGQNHEMTVVEADANYVQPFITSSIDVQSGETYSVLITTDQDPSINYWLSAAVTGHNLAT 160
           E QNH+M VVEAD NY+QPF  + +D+ SGE+YSVLITTDQDPS NYWLS  V G   AT
Sbjct: 189 EPQNHKMVVVEADGNYLQPFAVNDLDIYSGESYSVLITTDQDPSKNYWLSIGVRGRLPAT 248

Query: 161 PPALTLLHYIPTPATKLPPSPPPPPPQWNDFDRLKQFANQLLAAAGSPQPPPKYNRRMSF 220
           PP L++L+Y PT  +K P SPPP  P+W+D++  K F+N + A  GSP+PP  Y+RR++ 
Sbjct: 249 PPGLSILNYQPTSVSKFPTSPPPVTPRWDDYNHSKTFSNSIFALMGSPKPPTSYDRRITL 308

Query: 221 LTTQNENDGVLKWAINNVSLSLPTTPYLGAIKFGLNRSFDQFTPPETFPENYDIDIPLIN 280
           L TQN+ DG  KWAINNVSL+LP TPYLG+IK+GL  +FDQ +PPE FP+NYD+  P IN
Sbjct: 309 LNTQNKIDGFTKWAINNVSLALPPTPYLGSIKYGLQNAFDQKSPPENFPDNYDVMRPPIN 368

Query: 281 NATAVSSAIYRLEFGSTVDIVLQNANTLVKNVGEIHPWHLHGHDFWVLGFGPFGEGKFSA 340
             + + S +Y     STVD++LQNAN+L  NV EIHPWHLHGHDFWVLG   +GEGKFSA
Sbjct: 369 PNSTIGSGVYMFGLNSTVDVILQNANSLSNNVSEIHPWHLHGHDFWVLG---YGEGKFSA 428

Query: 341 ADEKLLNLKNPALKNTVVVFPYGWTAVRFVADNPGVWIFHCHIEPHLHMGMGVVIAEGVD 400
            DEK LN KNP L+NT V+FPYGWTA+R+VADNPGVW FHCHIEPHLHMGMGVV AEGV 
Sbjct: 429 KDEKKLNFKNPPLRNTAVIFPYGWTALRYVADNPGVWAFHCHIEPHLHMGMGVVFAEGVH 488

Query: 401 RVGDIPKNALSCGLTGQ 418
            V  IP  AL+CGLT +
Sbjct: 489 HVKSIPNEALTCGLTAK 502

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BLAST of Spg022635 vs. NCBI nr
Match: KAG7947369.1 (hypothetical protein I3843_14G091000 [Carya illinoinensis])

HSP 1 Score: 443.0 bits (1138), Expect = 2.9e-120
Identity = 202/317 (63.72%), Postives = 243/317 (76.66%), Query Frame = 0


Query: 101 EGQNHEMTVVEADANYVQPFITSSIDVQSGETYSVLITTDQDPSINYWLSAAVTGHNLAT 160
           E QNH+M VVEAD NY+QPF  + +D+ SGE+YSVLITTDQDPS NYWLS  V G   AT
Sbjct: 202 EPQNHKMVVVEADGNYLQPFAVNDLDIYSGESYSVLITTDQDPSKNYWLSIGVRGRLPAT 261

Query: 161 PPALTLLHYIPTPATKLPPSPPPPPPQWNDFDRLKQFANQLLAAAGSPQPPPKYNRRMSF 220
           PP L++L+Y PT  +K P SPPP  P+W+D++  K F+N + A  GSP+PP  Y+RR++ 
Sbjct: 262 PPGLSILNYQPTSVSKFPTSPPPVTPRWDDYNHSKTFSNSIFALMGSPKPPTSYDRRITL 321

Query: 221 LTTQNENDGVLKWAINNVSLSLPTTPYLGAIKFGLNRSFDQFTPPETFPENYDIDIPLIN 280
           L TQN+ DG  KWAINNVSL+LP TPYLG+IK+GL  +FDQ +PPE FP+NYD+  P IN
Sbjct: 322 LNTQNKIDGFTKWAINNVSLALPPTPYLGSIKYGLQNAFDQKSPPENFPDNYDVMRPPIN 381

Query: 281 NATAVSSAIYRLEFGSTVDIVLQNANTLVKNVGEIHPWHLHGHDFWVLGFGPFGEGKFSA 340
             + + S +Y     STVD++LQNAN+L  NV EIHPWHLHGHDFWVLG   +GEGKFSA
Sbjct: 382 PNSTIGSGVYMFGLNSTVDVILQNANSLSNNVSEIHPWHLHGHDFWVLG---YGEGKFSA 441

Query: 341 ADEKLLNLKNPALKNTVVVFPYGWTAVRFVADNPGVWIFHCHIEPHLHMGMGVVIAEGVD 400
            DEK LN KNP L+NT V+FPYGWTA+R+VADNPGVW FHCHIEPHLHMGMGVV AEGV 
Sbjct: 442 KDEKKLNFKNPPLRNTAVIFPYGWTALRYVADNPGVWAFHCHIEPHLHMGMGVVFAEGVH 501

Query: 401 RVGDIPKNALSCGLTGQ 418
            V  IP  AL+CGLT +
Sbjct: 502 HVKSIPNEALTCGLTAK 515

[Close]

BLAST of Spg022635 vs. NCBI nr
Match: XP_035542995.1 (L-ascorbate oxidase-like [Juglans regia] >KAF5441951.1 hypothetical protein F2P56_037098 [Juglans regia])

HSP 1 Score: 441.8 bits (1135), Expect = 6.5e-120
Identity = 206/327 (63.00%), Postives = 244/327 (74.62%), Query Frame = 0


Query: 91  SSTNLLLVTREGQNHEMTVVEADANYVQPFITSSIDVQSGETYSVLITTDQDPSINYWLS 150
           SST L  +     NH+M VVEAD NY+QPF  + +D+ SGE+YSVLITTDQDPS NYWLS
Sbjct: 257 SSTALASLNLAIGNHKMVVVEADGNYLQPFAVNDLDIYSGESYSVLITTDQDPSKNYWLS 316

Query: 151 AAVTGHNLATPPALTLLHYIPTPATKLPPSPPPPPPQWNDFDRLKQFANQLLAAAGSPQP 210
             V G   ATPPALT+L+Y P  A+K P SPPP  P+WND+D  K+F+  + A  GSP+P
Sbjct: 317 LGVRGRLPATPPALTILNYQPISASKFPTSPPPVTPRWNDYDHSKKFSKSIFALMGSPKP 376

Query: 211 PPKYNRRMSFLTTQNENDGVLKWAINNVSLSLPTTPYLGAIKFGLNRSFDQFTPPETFPE 270
           P  Y+RR++ L TQN+ DG  KWAINNVSL+LP TPYLG+IK+GL  +FDQ +PPE FP 
Sbjct: 377 PKSYDRRITLLNTQNKIDGFTKWAINNVSLALPPTPYLGSIKYGLRNAFDQKSPPENFPN 436

Query: 271 NYDIDIPLINNATAVSSAIYRLEFGSTVDIVLQNANTLVKNVGEIHPWHLHGHDFWVLGF 330
           +YD+  P IN  +   S +Y     +TVD++LQNAN L  NV EIHPWHLHGHDFWVLG 
Sbjct: 437 SYDVTKPPINPNSTTGSGVYMFGLNTTVDVILQNANALSDNVSEIHPWHLHGHDFWVLG- 496

Query: 331 GPFGEGKFSAADEKLLNLKNPALKNTVVVFPYGWTAVRFVADNPGVWIFHCHIEPHLHMG 390
             +GEGKFSA DEK LN KNP L+NT V+FPYGWTA+R+VADNPGVW FHCHIEPHLHMG
Sbjct: 497 --YGEGKFSAKDEKKLNFKNPPLRNTAVIFPYGWTALRYVADNPGVWAFHCHIEPHLHMG 556

Query: 391 MGVVIAEGVDRVGDIPKNALSCGLTGQ 418
           MGVV AEGV  V  IP  ALSCGLT +
Sbjct: 557 MGVVFAEGVHHVKSIPNEALSCGLTAK 580

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BLAST of Spg022635 vs. ExPASy Swiss-Prot
Match: P24792 (L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2)

HSP 1 Score: 437.6 bits (1124), Expect = 1.6e-121
Identity = 203/327 (62.08%), Postives = 247/327 (75.54%), Query Frame = 0


Query: 91  SSTNLLLVTREGQNHEMTVVEADANYVQPFITSSIDVQSGETYSVLITTDQDPSINYWLS 150
           S+T L  +     NH + VVEAD NYVQPF TS ID+ SGE+YSVLITTDQ+PS NYW+S
Sbjct: 250 STTALAALNFAIGNHPLLVVEADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVS 309

Query: 151 AAVTGHNLATPPALTLLHYIPTPATKLPPSPPPPPPQWNDFDRLKQFANQLLAAAGSPQP 210
               G +  TPP LTLL+Y+P   +KLP SPPP  P W+DFDR K F  ++ AA GSP+P
Sbjct: 310 VGTRGRHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNFTYRITAAMGSPKP 369

Query: 211 PPKYNRRMSFLTTQNENDGVLKWAINNVSLSLPTTPYLGAIKFGLNRSFDQFTPPETFPE 270
           P K NRR+  L TQN  +G +KWAIN+VSL+LP TPYLGA+KF L  +FDQ  PPE FPE
Sbjct: 370 PVKSNRRIFLLNTQNVINGYVKWAINDVSLALPPTPYLGAMKFNLLHAFDQNPPPEVFPE 429

Query: 271 NYDIDIPLINNATAVSSAIYRLEFGSTVDIVLQNANTLVKNVGEIHPWHLHGHDFWVLGF 330
           +YDID P  N  T + + +Y+ + G  VD++LQNAN + +N+ EIHPWHLHGHDFWVLG 
Sbjct: 430 DYDIDTPPTNEKTKIGNGVYQFKIGEIVDVILQNANMMKENLSEIHPWHLHGHDFWVLG- 489

Query: 331 GPFGEGKFSAADEKLLNLKNPALKNTVVVFPYGWTAVRFVADNPGVWIFHCHIEPHLHMG 390
             +G+GKF+A +E  LNLKNP L+NTVV+FPYGWTA+RFVADNPGVW FHCHIEPHLHMG
Sbjct: 490 --YGDGKFTAEEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMG 549

Query: 391 MGVVIAEGVDRVGDIPKNALSCGLTGQ 418
           MGVV AEGV++VG IP  AL+CG T +
Sbjct: 550 MGVVFAEGVEKVGRIPTKALACGGTAK 573

[Close]

BLAST of Spg022635 vs. ExPASy Swiss-Prot
Match: P37064 (L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1)

HSP 1 Score: 436.8 bits (1122), Expect = 2.8e-121
Identity = 201/327 (61.47%), Postives = 247/327 (75.54%), Query Frame = 0


Query: 91  SSTNLLLVTREGQNHEMTVVEADANYVQPFITSSIDVQSGETYSVLITTDQDPSINYWLS 150
           S+T L  +     NH++ VVEAD NYVQPF TS ID+ SGE+YSVLITTDQ+PS NYW+S
Sbjct: 220 STTALAALNFAIGNHQLLVVEADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVS 279

Query: 151 AAVTGHNLATPPALTLLHYIPTPATKLPPSPPPPPPQWNDFDRLKQFANQLLAAAGSPQP 210
                 +  TPP LTLL+Y+P   +KLP SPPP  P W+DFDR K F  ++ AA GSP+P
Sbjct: 280 VGTRARHPNTPPGLTLLNYLPNSVSKLPTSPPPQTPAWDDFDRSKNFTYRITAAMGSPKP 339

Query: 211 PPKYNRRMSFLTTQNENDGVLKWAINNVSLSLPTTPYLGAIKFGLNRSFDQFTPPETFPE 270
           P K+NRR+  L TQN  +G +KWAIN+VSL+LP TPYLGA+K+ L  +FDQ  PPE FPE
Sbjct: 340 PVKFNRRIFLLNTQNVINGYVKWAINDVSLALPPTPYLGAMKYNLLHAFDQNPPPEVFPE 399

Query: 271 NYDIDIPLINNATAVSSAIYRLEFGSTVDIVLQNANTLVKNVGEIHPWHLHGHDFWVLGF 330
           +YDID P  N  T + + +Y+ + G  VD++LQNAN + +N+ E HPWHLHGHDFWVLG 
Sbjct: 400 DYDIDTPPTNEKTRIGNGVYQFKIGEVVDVILQNANMMKENLSETHPWHLHGHDFWVLG- 459

Query: 331 GPFGEGKFSAADEKLLNLKNPALKNTVVVFPYGWTAVRFVADNPGVWIFHCHIEPHLHMG 390
             +G+GKFSA +E  LNLKNP L+NTVV+FPYGWTA+RFVADNPGVW FHCHIEPHLHMG
Sbjct: 460 --YGDGKFSAEEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMG 519

Query: 391 MGVVIAEGVDRVGDIPKNALSCGLTGQ 418
           MGVV AEGV++VG IP  AL+CG T +
Sbjct: 520 MGVVFAEGVEKVGRIPTKALACGGTAK 543

[Close]

BLAST of Spg022635 vs. ExPASy Swiss-Prot
Match: P14133 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 3.0e-120
Identity = 205/313 (65.50%), Postives = 237/313 (75.72%), Query Frame = 0


Query: 104 NHEMTVVEADANYVQPFITSSIDVQSGETYSVLITTDQDPSINYWLSAAVTGHNLATPPA 163
           NHE+ VVEAD NYVQPF+TS ID+ SGE+YSVLITTDQ+P  NYW+S  V      TPP 
Sbjct: 268 NHELLVVEADGNYVQPFVTSDIDIYSGESYSVLITTDQNPLENYWVSIGVRARLPKTPPG 327

Query: 164 LTLLHYIPTPATKLPPSPPPPPPQWNDFDRLKQFANQLLAAAGSPQPPPKYNRRMSFLTT 223
           LTLL+Y+P  A+KLP SPPP  P W DFDR K F  ++ AA GSP+PP +YNRR+  L T
Sbjct: 328 LTLLNYLPNSASKLPISPPPETPHWEDFDRSKNFTFRIFAAMGSPKPPVRYNRRLFLLNT 387

Query: 224 QNENDGVLKWAINNVSLSLPTTPYLGAIKFGLNRSFDQFTPPETFPENYDIDIPLINNAT 283
           QN  +G +KWAINNVSL+LP TPYL A+K  LN +F+Q  PPETFP NYDI+ P  N  T
Sbjct: 388 QNRINGFMKWAINNVSLALPPTPYLAAMKMRLNTAFNQNPPPETFPLNYDINNPPPNPET 447

Query: 284 AVSSAIYRLEFGSTVDIVLQNANTLVKNVGEIHPWHLHGHDFWVLGFGPFGEGKFSA-AD 343
              + +Y+   G TVD++LQNAN L  N+ EIHPWHLHGHDFWVLG   +GEGKF A  D
Sbjct: 448 TTGNGVYKFNMGETVDVILQNANMLNPNMSEIHPWHLHGHDFWVLG---YGEGKFYAPED 507

Query: 344 EKLLNLKNPALKNTVVVFPYGWTAVRFVADNPGVWIFHCHIEPHLHMGMGVVIAEGVDRV 403
           EK LNLKNP L+NTVV+FPYGWTA+RFVADNPGVW FHCHIEPHLHMGMGVV AEGV  V
Sbjct: 508 EKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVHMV 567

Query: 404 GDIPKNALSCGLT 416
           G IP  AL+CG T
Sbjct: 568 GMIPPKALACGST 577

[Close]

BLAST of Spg022635 vs. ExPASy Swiss-Prot
Match: Q40588 (L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 1.8e-112
Identity = 199/367 (54.22%), Postives = 250/367 (68.12%), Query Frame = 0


Query: 57  RIPKNPRSMSSIGGGVASTTPVCRFSV-----LQVTSSPSSTNLLLVTREGQNHEMTVVE 116
           R  K P     + GG      + R        L+V S+ +  +L L       H+M VVE
Sbjct: 212 RFSKPPLPQCKLRGGEQYAPQILRVRPNKIYRLRVASTTALGSLSLAI---GGHKMVVVE 271

Query: 117 ADANYVQPFITSSIDVQSGETYSVLITTDQDPSINYWLSAAVTGHNLATPPALTLLHYIP 176
           AD NYVQPF    +D+ SGE+YSVL  TDQDP+ NYW+S  V G    TP  LTLL+Y+P
Sbjct: 272 ADGNYVQPFSVQDMDIYSGESYSVLFKTDQDPTKNYWISINVRGREPKTPQGLTLLNYLP 331

Query: 177 TPATKLPPSPPPPPPQWNDFDRLKQFANQLLAAAGSPQPPPKYNRRMSFLTTQNENDGVL 236
             A+K P  PPP  P WND++  K F+N++ A  GSP+PPP+ +RR+  L TQN+ DG  
Sbjct: 332 NSASKFPTLPPPIAPLWNDYNHSKSFSNKIFALMGSPKPPPQNHRRIILLNTQNKIDGYT 391

Query: 237 KWAINNVSLSLPTTPYLGAIKFGLNRSFDQFTPPETFPENYDIDIPLINNATAVSSAIYR 296
           KWAINNVSL LPT  YLG+I++G+N +FD   PP+ FP++YD+     N+ +   + +Y 
Sbjct: 392 KWAINNVSLVLPTQLYLGSIRYGIN-AFDTKPPPDNFPKDYDVLKQAPNSNSTYGNGVYM 451

Query: 297 LEFGSTVDIVLQNANTLVKNVGEIHPWHLHGHDFWVLGFGPFGEGKFSAADEKLLNLKNP 356
           L+F +T+DI+LQNAN L K+V EIHPWHLHGHDFWVLG   +GEGKFS  D K  NLKNP
Sbjct: 452 LKFNTTIDIILQNANALAKDVSEIHPWHLHGHDFWVLG---YGEGKFSEKDVKKFNLKNP 511

Query: 357 ALKNTVVVFPYGWTAVRFVADNPGVWIFHCHIEPHLHMGMGVVIAEGVDRVGDIPKNALS 416
            L+NT V+FP+GWTA+RFV DNPGVW FHCHIEPHLHMGMGV+ AEGV  V  IPK AL+
Sbjct: 512 PLRNTAVIFPFGWTALRFVTDNPGVWAFHCHIEPHLHMGMGVIFAEGVHLVKKIPKEALA 571

Query: 417 CGLTGQM 419
           CGLTG+M
Sbjct: 572 CGLTGKM 571

[Close]

BLAST of Spg022635 vs. ExPASy Swiss-Prot
Match: M4DUF2 (L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 5.2e-112
Identity = 189/328 (57.62%), Postives = 235/328 (71.65%), Query Frame = 0


Query: 91  SSTNLLLVTREGQNHEMTVVEADANYVQPFITSSIDVQSGETYSVLITTDQDPSINYWLS 150
           SST L  +    Q H++ VVEAD NY+ PF T  ID+ SGETYSVL+TTDQDPS NY+++
Sbjct: 238 SSTALASLNFAVQGHKLVVVEADGNYITPFTTDDIDIYSGETYSVLLTTDQDPSQNYYIT 297

Query: 151 AAVTGHNLATPPALTLLHYIPTPATKLPPSPPPPPPQWNDFDRLKQFANQLLAAAGSPQP 210
           A V G    TPPALT+L+Y+  P+++LP SPPP  P+WNDFDR K F+ ++ AA GSP P
Sbjct: 298 AGVRGRKPNTPPALTVLNYVTAPSSQLPTSPPPETPRWNDFDRSKNFSKKIFAAMGSPSP 357

Query: 211 PPKYNRRMSFLTTQNENDGVLKWAINNVSLSLPTTPYLGAIKFGLNRSFDQFTPPETFPE 270
           P  ++ R+  L TQN  +G  KWAINNVSL++P TPYLG++K+ L   F++ +PP+ +P 
Sbjct: 358 PETFDERLILLNTQNLIEGFTKWAINNVSLAVPGTPYLGSVKYNLRTGFNRSSPPKDYPV 417

Query: 271 NYDIDIPLINNATAVSSAIYRLEFGSTVDIVLQNANTLVKNVGEIHPWHLHGHDFWVLGF 330
           +YDI  P  N      +      F  TVD++LQNAN L  N  EIHPWHLHGHDFWVLG 
Sbjct: 418 DYDIMTPPRNRNAKQGNVSCVFPFNVTVDVILQNANGLNANASEIHPWHLHGHDFWVLG- 477

Query: 331 GPFGEGKFS-AADEKLLNLKNPALKNTVVVFPYGWTAVRFVADNPGVWIFHCHIEPHLHM 390
             +GEGKF    DEK  NLKNP L+NTV ++PYGWTA+RFV DNPGVW FHCHIEPHLHM
Sbjct: 478 --YGEGKFKPGVDEKTYNLKNPPLRNTVALYPYGWTALRFVTDNPGVWFFHCHIEPHLHM 537

Query: 391 GMGVVIAEGVDRVGDIPKNALSCGLTGQ 418
           GMGVV AEG++R+G +P  AL CGLT Q
Sbjct: 538 GMGVVFAEGLNRIGKVPDEALGCGLTKQ 562

[Close]

BLAST of Spg022635 vs. ExPASy TrEMBL
Match: A0A660KQX3 (Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_010271 PE=3 SV=1)

HSP 1 Score: 446.0 bits (1146), Expect = 1.7e-121
Identity = 206/328 (62.80%), Postives = 251/328 (76.52%), Query Frame = 0


Query: 91  SSTNLLLVTREGQNHEMTVVEADANYVQPFITSSIDVQSGETYSVLITTDQDPSINYWLS 150
           S+T L  +     NH+M VVEAD NY+QPF    +D+ SGE+YSVLITT+QDPS NYW+S
Sbjct: 238 STTALASLNLAIGNHKMVVVEADGNYLQPFAVDDLDIYSGESYSVLITTNQDPSKNYWIS 297

Query: 151 AAVTGHNLATPPALTLLHYIPTPATKLPPSPPPPPPQWNDFDRLKQFANQLLAAAGSPQP 210
             V G + ATP  LT+L+Y PT A+KLP SPPP  P+WND+   K F+N++LA  GSP+P
Sbjct: 298 VGVRGRSPATPQGLTILNYKPTSASKLPSSPPPVTPRWNDYVHSKTFSNKILARMGSPRP 357

Query: 211 PPKYNRRMSFLTTQNENDGVLKWAINNVSLSLPTTPYLGAIKFGLNRSFDQFTPPETFPE 270
           P KY+RR+  L TQN+ +G  KWA+NNVSL+LP TPYLG+IK+GL  +FDQ TPPE F +
Sbjct: 358 PTKYDRRIFLLNTQNKINGFTKWALNNVSLTLPATPYLGSIKYGLKNAFDQKTPPENFAD 417

Query: 271 NYDIDIPLINNATAVSSAIYRLEFGSTVDIVLQNANTLVKNVGEIHPWHLHGHDFWVLGF 330
           NYD+ +P IN  T+  S  Y +E  STVD++LQNAN L  NV EIHPWHLHGHDFWVLG 
Sbjct: 418 NYDVMVPPINPNTSTGSGAYIIELNSTVDLILQNANALSDNVSEIHPWHLHGHDFWVLG- 477

Query: 331 GPFGEGKFSAADEKLLNLKNPALKNTVVVFPYGWTAVRFVADNPGVWIFHCHIEPHLHMG 390
             +GEGKFS  DEK LNLKNP L+NT V+FPYGWTA+RFVADNPG W FHCHIEPHLHMG
Sbjct: 478 --YGEGKFSEKDEKKLNLKNPPLRNTAVIFPYGWTALRFVADNPGAWAFHCHIEPHLHMG 537

Query: 391 MGVVIAEGVDRVGDIPKNALSCGLTGQM 419
           MGVV AEG+ RV +IP+ A++CGLT +M
Sbjct: 538 MGVVFAEGIHRVDNIPREAIACGLTAKM 562

[Close]

BLAST of Spg022635 vs. ExPASy TrEMBL
Match: A0A6P9E4J4 (L-ascorbate oxidase OS=Juglans regia OX=51240 GN=LOC108986886 PE=3 SV=1)

HSP 1 Score: 441.8 bits (1135), Expect = 3.2e-120
Identity = 206/327 (63.00%), Postives = 244/327 (74.62%), Query Frame = 0


Query: 91  SSTNLLLVTREGQNHEMTVVEADANYVQPFITSSIDVQSGETYSVLITTDQDPSINYWLS 150
           SST L  +     NH+M VVEAD NY+QPF  + +D+ SGE+YSVLITTDQDPS NYWLS
Sbjct: 257 SSTALASLNLAIGNHKMVVVEADGNYLQPFAVNDLDIYSGESYSVLITTDQDPSKNYWLS 316

Query: 151 AAVTGHNLATPPALTLLHYIPTPATKLPPSPPPPPPQWNDFDRLKQFANQLLAAAGSPQP 210
             V G   ATPPALT+L+Y P  A+K P SPPP  P+WND+D  K+F+  + A  GSP+P
Sbjct: 317 LGVRGRLPATPPALTILNYQPISASKFPTSPPPVTPRWNDYDHSKKFSKSIFALMGSPKP 376

Query: 211 PPKYNRRMSFLTTQNENDGVLKWAINNVSLSLPTTPYLGAIKFGLNRSFDQFTPPETFPE 270
           P  Y+RR++ L TQN+ DG  KWAINNVSL+LP TPYLG+IK+GL  +FDQ +PPE FP 
Sbjct: 377 PKSYDRRITLLNTQNKIDGFTKWAINNVSLALPPTPYLGSIKYGLRNAFDQKSPPENFPN 436

Query: 271 NYDIDIPLINNATAVSSAIYRLEFGSTVDIVLQNANTLVKNVGEIHPWHLHGHDFWVLGF 330
           +YD+  P IN  +   S +Y     +TVD++LQNAN L  NV EIHPWHLHGHDFWVLG 
Sbjct: 437 SYDVTKPPINPNSTTGSGVYMFGLNTTVDVILQNANALSDNVSEIHPWHLHGHDFWVLG- 496

Query: 331 GPFGEGKFSAADEKLLNLKNPALKNTVVVFPYGWTAVRFVADNPGVWIFHCHIEPHLHMG 390
             +GEGKFSA DEK LN KNP L+NT V+FPYGWTA+R+VADNPGVW FHCHIEPHLHMG
Sbjct: 497 --YGEGKFSAKDEKKLNFKNPPLRNTAVIFPYGWTALRYVADNPGVWAFHCHIEPHLHMG 556

Query: 391 MGVVIAEGVDRVGDIPKNALSCGLTGQ 418
           MGVV AEGV  V  IP  ALSCGLT +
Sbjct: 557 MGVVFAEGVHHVKSIPNEALSCGLTAK 580

[Close]

BLAST of Spg022635 vs. ExPASy TrEMBL
Match: A0A6P9EUV6 (L-ascorbate oxidase OS=Juglans regia OX=51240 GN=LOC108986893 PE=3 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 5.4e-120
Identity = 206/327 (63.00%), Postives = 244/327 (74.62%), Query Frame = 0


Query: 91  SSTNLLLVTREGQNHEMTVVEADANYVQPFITSSIDVQSGETYSVLITTDQDPSINYWLS 150
           SST L  +     NH+M VVEAD NY+QPF  + +D+ SGE+YSVLITTDQDPS NYWLS
Sbjct: 257 SSTALASLNLAIGNHKMVVVEADGNYLQPFAVNDLDIYSGESYSVLITTDQDPSKNYWLS 316

Query: 151 AAVTGHNLATPPALTLLHYIPTPATKLPPSPPPPPPQWNDFDRLKQFANQLLAAAGSPQP 210
             V G   ATPPALT+L+Y P  A+K P SPPP  P+WND+D  K+F+  + A  GSP+P
Sbjct: 317 LGVRGRLPATPPALTILNYQPISASKFPTSPPPVTPRWNDYDHSKKFSKSIFALMGSPKP 376

Query: 211 PPKYNRRMSFLTTQNENDGVLKWAINNVSLSLPTTPYLGAIKFGLNRSFDQFTPPETFPE 270
           P  Y+RR++ L TQN+ DG  KWAINNVSL+LP TPYLG+IK+GL  +FDQ +PPE FP 
Sbjct: 377 PKSYDRRITLLNTQNKIDGFTKWAINNVSLALPPTPYLGSIKYGLRNAFDQKSPPENFPN 436

Query: 271 NYDIDIPLINNATAVSSAIYRLEFGSTVDIVLQNANTLVKNVGEIHPWHLHGHDFWVLGF 330
           +YD+  P IN  +   S +Y     +TVD++LQNAN L  NV EIHPWHLHGHDFWVLG 
Sbjct: 437 SYDVMKPPINPNSTTGSGVYMFGLNTTVDVILQNANALSDNVSEIHPWHLHGHDFWVLG- 496

Query: 331 GPFGEGKFSAADEKLLNLKNPALKNTVVVFPYGWTAVRFVADNPGVWIFHCHIEPHLHMG 390
             +GEGKFSA DEK LN KNP L+NT V+FPYGWTA+R+VADNPGVW FHCHIEPHLHMG
Sbjct: 497 --YGEGKFSAKDEKKLNFKNPPLRNTAVIFPYGWTALRYVADNPGVWAFHCHIEPHLHMG 556

Query: 391 MGVVIAEGVDRVGDIPKNALSCGLTGQ 418
           MGVV AEGV  V  IP  ALSCGLT +
Sbjct: 557 MGVVFAEGVHHVKSIPNEALSCGLTAK 580

[Close]

BLAST of Spg022635 vs. ExPASy TrEMBL
Match: A0A6J1ENQ7 (L-ascorbate oxidase OS=Cucurbita moschata OX=3662 GN=LOC111436167 PE=3 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 9.2e-120
Identity = 202/327 (61.77%), Postives = 248/327 (75.84%), Query Frame = 0


Query: 91  SSTNLLLVTREGQNHEMTVVEADANYVQPFITSSIDVQSGETYSVLITTDQDPSINYWLS 150
           S+T L  +     NH++ VVEAD NYVQPF TS ID+ SGE+YSVLITTDQ+PS NYW+S
Sbjct: 250 STTALAALNFAIGNHQLLVVEADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVS 309

Query: 151 AAVTGHNLATPPALTLLHYIPTPATKLPPSPPPPPPQWNDFDRLKQFANQLLAAAGSPQP 210
                 +  TPP LTLL+Y+P   +KLP SPPP  P W+DFDR K F  ++ AA GSP+P
Sbjct: 310 VGTRARHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNFTYRITAAMGSPKP 369

Query: 211 PPKYNRRMSFLTTQNENDGVLKWAINNVSLSLPTTPYLGAIKFGLNRSFDQFTPPETFPE 270
           P K+NRR+  L TQN  +G +KWAIN+VSL+LP TPYLGA+K+ L  +FDQ  PPE FPE
Sbjct: 370 PVKFNRRIFLLNTQNVINGYVKWAINDVSLALPPTPYLGAMKYNLLHAFDQNPPPEVFPE 429

Query: 271 NYDIDIPLINNATAVSSAIYRLEFGSTVDIVLQNANTLVKNVGEIHPWHLHGHDFWVLGF 330
           +YDID P  N  T + + +Y+ + G  VD++LQNAN + +N+ E HPWHLHGHDFWVLG 
Sbjct: 430 DYDIDTPPTNEKTRIGNGVYQFKIGEVVDVILQNANMMKENLSETHPWHLHGHDFWVLG- 489

Query: 331 GPFGEGKFSAADEKLLNLKNPALKNTVVVFPYGWTAVRFVADNPGVWIFHCHIEPHLHMG 390
             +G+GKFSA +E  LNLKNP L+NTVV+FPYGWTA+RFVADNPGVW FHCHIEPHLHMG
Sbjct: 490 --YGDGKFSAEEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMG 549

Query: 391 MGVVIAEGVDRVGDIPKNALSCGLTGQ 418
           MGVV AEGVD+VG IP  AL+CG+T +
Sbjct: 550 MGVVFAEGVDKVGRIPTKALACGVTAK 573

[Close]

BLAST of Spg022635 vs. ExPASy TrEMBL
Match: A0A5J5C7U3 (L-ascorbate oxidase OS=Nyssa sinensis OX=561372 GN=F0562_001734 PE=4 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 1.6e-119
Identity = 206/341 (60.41%), Postives = 253/341 (74.19%), Query Frame = 0


Query: 78  VCRFSVLQVTSSPSSTNLLLVTREGQNHEMTVVEADANYVQPFITSSIDVQSGETYSVLI 137
           V R  +  +TS  SS NL +      NH+M VVEAD NY+QPF   ++D+ SGE+YSVL 
Sbjct: 20  VYRLRIASITSM-SSLNLAI-----GNHKMVVVEADGNYLQPFAVDNMDIYSGESYSVLF 79

Query: 138 TTDQDPSINYWLSAAVTGHNLATPPALTLLHYIPTPATKLPPSPPPPPPQWNDFDRLKQF 197
           TTDQDPS NYW+S +V G    TP  LT+L+Y PT A+KLP S PP  PQWND++  K F
Sbjct: 80  TTDQDPSKNYWISISVRGREPKTPQGLTILNYHPTSASKLPTSSPPVAPQWNDYNHSKSF 139

Query: 198 ANQLLAAAGSPQPPPKYNRRMSFLTTQNENDGVLKWAINNVSLSLPTTPYLGAIKFGLNR 257
           ++++LA  GSP+PP  YNRR+  L TQN  +G +KWAINN+SLSLP TPYLG+I++GLN 
Sbjct: 140 SHKILAIMGSPKPPTTYNRRIMLLNTQNYINGYIKWAINNISLSLPATPYLGSIRYGLNN 199

Query: 258 SFDQFTPPETFPENYDIDIPLINNATAVSSAIYRLEFGSTVDIVLQNANTLVKNVGEIHP 317
           SFDQ +PP++F   YD+  P IN  +   S +Y L F +TVD++LQNAN L +N  EIHP
Sbjct: 200 SFDQTSPPDSFSHFYDVMKPPINPNSTYGSGVYMLAFNTTVDVILQNANALSENTSEIHP 259

Query: 318 WHLHGHDFWVLGFGPFGEGKFSAADEKLLNLKNPALKNTVVVFPYGWTAVRFVADNPGVW 377
           WHLHGHDFWVLG   +G+GKFS  DEK  NLKNP L+NT V+FP+GWTA+RFVADNPGVW
Sbjct: 260 WHLHGHDFWVLG---YGDGKFSVKDEKKFNLKNPPLRNTAVIFPFGWTALRFVADNPGVW 319

Query: 378 IFHCHIEPHLHMGMGVVIAEGVDRVGDIPKNALSCGLTGQM 419
            FHCHIEPHLHMGMGVV AEGV  VG +P  ALSCG+TG+M
Sbjct: 320 AFHCHIEPHLHMGMGVVFAEGVHHVGKVPNEALSCGMTGKM 351

[Close]

BLAST of Spg022635 vs. TAIR 10
Match: AT5G21105.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 396.7 bits (1018), Expect = 2.2e-110
Identity = 185/328 (56.40%), Postives = 232/328 (70.73%), Query Frame = 0


Query: 91  SSTNLLLVTREGQNHEMTVVEADANYVQPFITSSIDVQSGETYSVLITTDQDPSINYWLS 150
           S+T L  +    Q H++ VVEAD NY+ PF T  ID+ SGE+YSVL+TTDQDPS NY++S
Sbjct: 256 STTALASLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYIS 315

Query: 151 AAVTGHNLATPPALTLLHYIPTPATKLPPSPPPPPPQWNDFDRLKQFANQLLAAAGSPQP 210
             V G    T  ALT+L+Y+  PA+KLP SPPP  P+W+DF+R K F+ ++ +A GSP P
Sbjct: 316 VGVRGRKPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSP 375

Query: 211 PPKYNRRMSFLTTQNENDGVLKWAINNVSLSLPTTPYLGAIKFGLNRSFDQFTPPETFPE 270
           P KY +R+  L TQN  DG  KWAINNVSL  P TPYLG++K+ L   F++ +PP ++  
Sbjct: 376 PKKYRKRLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRM 435

Query: 271 NYDIDIPLINNATAVSSAIYRLEFGSTVDIVLQNANTLVKNVGEIHPWHLHGHDFWVLGF 330
           +YDI  P     T   + IY   F  TVD+++QNAN L   V EIHPWHLHGHDFWVLG 
Sbjct: 436 DYDIMNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLG- 495

Query: 331 GPFGEGKFS-AADEKLLNLKNPALKNTVVVFPYGWTAVRFVADNPGVWIFHCHIEPHLHM 390
             +G+GKF    DEK  NLKNP L+NT +++PYGWTA+RFV DNPGVW FHCHIEPHLHM
Sbjct: 496 --YGDGKFKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHM 555

Query: 391 GMGVVIAEGVDRVGDIPKNALSCGLTGQ 418
           GMGVV AEG++R+G +P  AL CGLT Q
Sbjct: 556 GMGVVFAEGLNRIGKVPDEALGCGLTKQ 580

[Close]

BLAST of Spg022635 vs. TAIR 10
Match: AT5G21105.2 (Plant L-ascorbate oxidase )

HSP 1 Score: 396.7 bits (1018), Expect = 2.2e-110
Identity = 185/328 (56.40%), Postives = 232/328 (70.73%), Query Frame = 0


Query: 91  SSTNLLLVTREGQNHEMTVVEADANYVQPFITSSIDVQSGETYSVLITTDQDPSINYWLS 150
           S+T L  +    Q H++ VVEAD NY+ PF T  ID+ SGE+YSVL+TTDQDPS NY++S
Sbjct: 65  STTALASLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYIS 124

Query: 151 AAVTGHNLATPPALTLLHYIPTPATKLPPSPPPPPPQWNDFDRLKQFANQLLAAAGSPQP 210
             V G    T  ALT+L+Y+  PA+KLP SPPP  P+W+DF+R K F+ ++ +A GSP P
Sbjct: 125 VGVRGRKPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSP 184

Query: 211 PPKYNRRMSFLTTQNENDGVLKWAINNVSLSLPTTPYLGAIKFGLNRSFDQFTPPETFPE 270
           P KY +R+  L TQN  DG  KWAINNVSL  P TPYLG++K+ L   F++ +PP ++  
Sbjct: 185 PKKYRKRLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRM 244

Query: 271 NYDIDIPLINNATAVSSAIYRLEFGSTVDIVLQNANTLVKNVGEIHPWHLHGHDFWVLGF 330
           +YDI  P     T   + IY   F  TVD+++QNAN L   V EIHPWHLHGHDFWVLG 
Sbjct: 245 DYDIMNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLG- 304

Query: 331 GPFGEGKFS-AADEKLLNLKNPALKNTVVVFPYGWTAVRFVADNPGVWIFHCHIEPHLHM 390
             +G+GKF    DEK  NLKNP L+NT +++PYGWTA+RFV DNPGVW FHCHIEPHLHM
Sbjct: 305 --YGDGKFKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHM 364

Query: 391 GMGVVIAEGVDRVGDIPKNALSCGLTGQ 418
           GMGVV AEG++R+G +P  AL CGLT Q
Sbjct: 365 GMGVVFAEGLNRIGKVPDEALGCGLTKQ 389

[Close]

BLAST of Spg022635 vs. TAIR 10
Match: AT5G21100.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 366.7 bits (940), Expect = 2.5e-101
Identity = 180/329 (54.71%), Postives = 224/329 (68.09%), Query Frame = 0


Query: 91  SSTNLLLVTREGQNHEMTVVEADANYVQPFITSSIDVQSGETYSVLITTDQDPSINYWLS 150
           S+T L  +    Q H++ VVEAD NYV PF  + IDV SGETYSVL+ T+  PS  YW+S
Sbjct: 239 STTALASLNLAVQGHQLVVVEADGNYVAPFTVNDIDVYSGETYSVLLKTNALPSKKYWIS 298

Query: 151 AAVTGHNLATPPALTLLHYIPTPATKLPPSPPPPPPQWNDFDRLKQFANQLLAAAGSPQP 210
             V G    TP ALT+++Y+    ++ P  PPP  P WND DR K F+ ++ AA G P+P
Sbjct: 299 VGVRGREPKTPQALTVINYVDATESR-PSHPPPVTPIWNDTDRSKSFSKKIFAAKGYPKP 358

Query: 211 PPKYNRRMSFLTTQNENDGVLKWAINNVSLSLPTTPYLGAIKFGLNRSFDQFTP-PETFP 270
           P K + ++  L TQN  +   KW+INNVSLS+P TPYLG+I++GL  ++D  +P  +   
Sbjct: 359 PEKSHDQLILLNTQNLYEDYTKWSINNVSLSVPVTPYLGSIRYGLKSAYDLKSPAKKLIM 418

Query: 271 ENYDIDIPLINNATAVSSAIYRLEFGSTVDIVLQNANTLVKNVGEIHPWHLHGHDFWVLG 330
           +NYDI  P  N  T   S IY   FG  VD++LQNAN L   + EIHPWH+HGHDFWVLG
Sbjct: 419 DNYDIMKPPPNPNTTKGSGIYNFAFGIVVDVILQNANVLKGVISEIHPWHIHGHDFWVLG 478

Query: 331 FGPFGEGKFS-AADEKLLNLKNPALKNTVVVFPYGWTAVRFVADNPGVWIFHCHIEPHLH 390
              +GEGKF    DEK  NLKNP L+NTVV++P+GWTA+RFV DNPGVW FHCHIEPHLH
Sbjct: 479 ---YGEGKFKPGIDEKTFNLKNPPLRNTVVLYPFGWTAIRFVTDNPGVWFFHCHIEPHLH 538

Query: 391 MGMGVVIAEGVDRVG--DIPKNALSCGLT 416
           MGMGVV  EGVDR+G  +IP  AL CGLT
Sbjct: 539 MGMGVVFVEGVDRIGKMEIPDEALGCGLT 563

[Close]

BLAST of Spg022635 vs. TAIR 10
Match: AT5G21105.3 (Plant L-ascorbate oxidase )

HSP 1 Score: 323.9 bits (829), Expect = 1.8e-88
Identity = 154/286 (53.85%), Postives = 197/286 (68.88%), Query Frame = 0


Query: 91  SSTNLLLVTREGQNHEMTVVEADANYVQPFITSSIDVQSGETYSVLITTDQDPSINYWLS 150
           S+T L  +    Q H++ VVEAD NY+ PF T  ID+ SGE+YSVL+TTDQDPS NY++S
Sbjct: 256 STTALASLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYIS 315

Query: 151 AAVTGHNLATPPALTLLHYIPTPATKLPPSPPPPPPQWNDFDRLKQFANQLLAAAGSPQP 210
             V G    T  ALT+L+Y+  PA+KLP SPPP  P+W+DF+R K F+ ++ +A GSP P
Sbjct: 316 VGVRGRKPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSP 375

Query: 211 PPKYNRRMSFLTTQNENDGVLKWAINNVSLSLPTTPYLGAIKFGLNRSFDQFTPPETFPE 270
           P KY +R+  L TQN  DG  KWAINNVSL  P TPYLG++K+ L   F++ +PP ++  
Sbjct: 376 PKKYRKRLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRM 435

Query: 271 NYDIDIPLINNATAVSSAIYRLEFGSTVDIVLQNANTLVKNVGEIHPWHLHGHDFWVLGF 330
           +YDI  P     T   + IY   F  TVD+++QNAN L   V EIHPWHLHGHDFWVLG 
Sbjct: 436 DYDIMNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLG- 495

Query: 331 GPFGEGKFS-AADEKLLNLKNPALKNTVVVFPYGWTAVRFVADNPG 376
             +G+GKF    DEK  NLKNP L+NT +++PYGWTA+RFV DNPG
Sbjct: 496 --YGDGKFKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPG 538

[Close]

BLAST of Spg022635 vs. TAIR 10
Match: AT4G39830.1 (Cupredoxin superfamily protein )

HSP 1 Score: 299.7 bits (766), Expect = 3.7e-81
Identity = 155/331 (46.83%), Postives = 203/331 (61.33%), Query Frame = 0


Query: 91  SSTNLLLVTREGQNHEMTVVEADANYVQPFITSSIDVQSGETYSVLITTDQDPSINYWLS 150
           S T L  ++ + + H +TVVEAD +YV+PF   ++ V SGETYSVL+  DQ+P  NYW++
Sbjct: 253 SLTALSALSFQIEGHNLTVVEADGHYVEPFTVKNLFVYSGETYSVLLKADQNPRRNYWIT 312

Query: 151 AAVTGHNLATPPALTLLHYIPTPATKLPPSPPPP--PPQWNDF-DRLKQFANQLLAAAGS 210
           +++      TPPA  +L+Y P    + PP+       P+WND   RL Q           
Sbjct: 313 SSIVSRPATTPPATAVLNYYPNHPRRRPPTSESSNIVPEWNDTRSRLAQSLAIKARRGFI 372

Query: 211 PQPPPKYNRRMSFLTTQNENDGVLKWAINNVSLSLPTTPYLGAIKFGLNRSFD-QFTPPE 270
              P   ++ +  L TQNE +G  +W++NNVS   P TPYL A+K  L  +FD +FT PE
Sbjct: 373 HALPENSDKVIVLLNTQNEVNGYRRWSVNNVSYHHPKTPYLIALKQNLTNAFDWRFTAPE 432

Query: 271 TF-PENYDIDIPLINNATAVSSAIYRLEFGSTVDIVLQNANTLVKNVGEIHPWHLHGHDF 330
            +   NYDI    +N     S  IYRL F STVD++LQNANT+  N  E HPWHLHGHDF
Sbjct: 433 NYDSRNYDIFAKPLNANATTSDGIYRLRFNSTVDVILQNANTMNANNSETHPWHLHGHDF 492

Query: 331 WVLGFGPFGEGKFS-AADEKLLNLKNPALKNTVVVFPYGWTAVRFVADNPGVWIFHCHIE 390
           WVLG   +GEGKF+ + D K  N  +P  KNTV V P+GWTA+RF ADNPGVW FHCHIE
Sbjct: 493 WVLG---YGEGKFNESEDPKRYNRVDPIKKNTVAVQPFGWTALRFRADNPGVWSFHCHIE 552

Query: 391 PHLHMGMGVVIAEGVDRVGDIPKNALSCGLT 416
            H  MGMG+V   G+D+V  +P + + CG T
Sbjct: 553 SHFFMGMGIVFESGIDKVSSLPSSIMGCGQT 580

The following BLAST results are available for this feature:

BLAST of Spg022635 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8037705.1	3.5e-121	62.80	hypothetical protein FH972_010271 [Carpinus fangiana]	[more]
KAG6671128.1	2.9e-120	63.72	hypothetical protein I3843_Q014000 [Carya illinoinensis]	[more]
KAG6671127.1	2.9e-120	63.72	hypothetical protein I3843_Q014000 [Carya illinoinensis]	[more]
KAG7947369.1	2.9e-120	63.72	hypothetical protein I3843_14G091000 [Carya illinoinensis]	[more]
XP_035542995.1	6.5e-120	63.00	L-ascorbate oxidase-like [Juglans regia] >KAF5441951.1 hypothetical protein F2P5...	[more]

BLAST of Spg022635 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P24792	1.6e-121	62.08	L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2	[more]
P37064	2.8e-121	61.47	L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1	[more]
P14133	3.0e-120	65.50	L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1	[more]
Q40588	1.8e-112	54.22	L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1	[more]
M4DUF2	5.2e-112	57.62	L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1	[more]

BLAST of Spg022635 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A660KQX3	1.7e-121	62.80	Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_010271 PE=3 SV=1	[more]
A0A6P9E4J4	3.2e-120	63.00	L-ascorbate oxidase OS=Juglans regia OX=51240 GN=LOC108986886 PE=3 SV=1	[more]
A0A6P9EUV6	5.4e-120	63.00	L-ascorbate oxidase OS=Juglans regia OX=51240 GN=LOC108986893 PE=3 SV=1	[more]
A0A6J1ENQ7	9.2e-120	61.77	L-ascorbate oxidase OS=Cucurbita moschata OX=3662 GN=LOC111436167 PE=3 SV=1	[more]
A0A5J5C7U3	1.6e-119	60.41	L-ascorbate oxidase OS=Nyssa sinensis OX=561372 GN=F0562_001734 PE=4 SV=1	[more]

BLAST of Spg022635 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G21105.1	2.2e-110	56.40	Plant L-ascorbate oxidase	[more]
AT5G21105.2	2.2e-110	56.40	Plant L-ascorbate oxidase	[more]
AT5G21100.1	2.5e-101	54.71	Plant L-ascorbate oxidase	[more]
AT5G21105.3	1.8e-88	53.85	Plant L-ascorbate oxidase	[more]
AT4G39830.1	3.7e-81	46.83	Cupredoxin superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001117	Multicopper oxidase, type 1	PFAM	PF00394	Cu-oxidase	coord: 103..170 e-value: 1.9E-18 score: 67.1
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 16..207 e-value: 8.0E-28 score: 99.8
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 208..417 e-value: 2.1E-70 score: 238.7
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 210..412
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 103..206
IPR011706	Multicopper oxidase, C-terminal	PFAM	PF07731	Cu-oxidase_2	coord: 280..399 e-value: 4.2E-35 score: 120.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..25
None	No IPR available	PANTHER	PTHR11709:SF394	PLANT L-ASCORBATE OXIDASE	coord: 91..415
IPR045087	Multicopper oxidase	PANTHER	PTHR11709	MULTI-COPPER OXIDASE	coord: 91..415
IPR033138	Multicopper oxidases, conserved site	PROSITE	PS00079	MULTICOPPER_OXIDASE1	coord: 375..395
IPR002355	Multicopper oxidase, copper-binding site	PROSITE	PS00080	MULTICOPPER_OXIDASE2	coord: 380..391
IPR034267	Ascorbate oxidase, third cupredoxin domain	CDD	cd13893	CuRO_3_AAO	coord: 215..406 e-value: 6.55329E-75 score: 228.844

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg022635.1	Spg022635.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0005507	copper ion binding
molecular_function	GO:0016491	oxidoreductase activity