Spg022631 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg022631
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	mitogen-activated protein kinase kinase kinase 17-like
Location	scaffold2: 6343782 .. 6344807 (-)
RNA-Seq Expression	Spg022631
Synteny	Spg022631

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACGAAGTCCTGTGTTATTTCACGAGTGAGTGAGCAAAGTCGTAACTTTGTGAACTTGCACGATTTCTTGAAAACGAAGAGAGGCATTGTGAAGTATGCAAAAAAGACACTGCCCGACAACAACTGGAACGACAGCGTCAAACATTACAATGGAAGATTTGCTAATTTGACCGTCGAAGAATCCCACGGTTCGTGTTGGATTGGAGATCGTCTTCTTCGTAAAAGGGGCTCTGGCCTTGTTTTCTTGGCCAAGAACAGAGAACCCACCAACCCAAATTCAGATCTTCCCACAGAAATCGTTGTCAAATCTTGTCTCATGGAATTCTCTTCTTCACTTCAACACGAGAAGGAAGTGCTCAGTGATTTGGCACCCTGCACTAATATAGTGCATTGCTACGGCGATGAAATCACAACGACAAAATCCGGTGAGAAAGTTTACAACGTCCTGTTCGAGTATTGCTCTGGCTCGAGCTTGCGTGACCACATTATTAACTTTGGACTTAATGGATTGCCGGAATACGAAGTTCGTAAGTATACAAGAGACATTGTTCATGGGCTCTATCACATGCATTGCAATGCTCGCTACGTCCACGGTGACATAAAACCAAGAAACATCTTATTGTTACCTAGTGCTTGCGCAGCTGACAAAGGTTCTCTAGTGGCTAAGATGGCCAGTTTTGGATTGGCTAAGAAGCTAACTCCTGAAGTAGTATGCGATCAAGAAATGAGTGGTTCGGGACCGTACATATCTCCAGAGTTGGCAAGGGATGGCTATTTAGATTGGCCTGCTGACATTTGGGCGCTTGGATGCGTGGTGTTGGAGATGTTGACTGGAAAACCAGCATGGAGCTTTGAGAATGCTAATAAGTACATGTTGGATTGCTACAACAATGAGCTGCTGCCAGAGATTCCGAAGGGGATGTCAAGTGCTGGAAGAGATTTTATAAAAAGGTGTTTGATTAGAAGTCCTTACAAAAGAAGACCGATTTGGTTGTTGATCAAACATCCTTTTGTTTGCCTTTGA

mRNA sequence

ATGACGAAGTCCTGTGTTATTTCACGAGTGAGTGAGCAAAGTCGTAACTTTGTGAACTTGCACGATTTCTTGAAAACGAAGAGAGGCATTGTGAAGTATGCAAAAAAGACACTGCCCGACAACAACTGGAACGACAGCGTCAAACATTACAATGGAAGATTTGCTAATTTGACCGTCGAAGAATCCCACGGTTCGTGTTGGATTGGAGATCGTCTTCTTCGTAAAAGGGGCTCTGGCCTTGTTTTCTTGGCCAAGAACAGAGAACCCACCAACCCAAATTCAGATCTTCCCACAGAAATCGTTGTCAAATCTTGTCTCATGGAATTCTCTTCTTCACTTCAACACGAGAAGGAAGTGCTCAGTGATTTGGCACCCTGCACTAATATAGTGCATTGCTACGGCGATGAAATCACAACGACAAAATCCGGTGAGAAAGTTTACAACGTCCTGTTCGAGTATTGCTCTGGCTCGAGCTTGCGTGACCACATTATTAACTTTGGACTTAATGGATTGCCGGAATACGAAGTTCGTAAGTATACAAGAGACATTGTTCATGGGCTCTATCACATGCATTGCAATGCTCGCTACGTCCACGGTGACATAAAACCAAGAAACATCTTATTGTTACCTAGTGCTTGCGCAGCTGACAAAGGTTCTCTAGTGGCTAAGATGGCCAGTTTTGGATTGGCTAAGAAGCTAACTCCTGAAGTAGTATGCGATCAAGAAATGAGTGGTTCGGGACCGTACATATCTCCAGAGTTGGCAAGGGATGGCTATTTAGATTGGCCTGCTGACATTTGGGCGCTTGGATGCGTGGTGTTGGAGATGTTGACTGGAAAACCAGCATGGAGCTTTGAGAATGCTAATAAGTACATGTTGGATTGCTACAACAATGAGCTGCTGCCAGAGATTCCGAAGGGGATGTCAAGTGCTGGAAGAGATTTTATAAAAAGGTGTTTGATTAGAAGTCCTTACAAAAGAAGACCGATTTGGTTGTTGATCAAACATCCTTTTGTTTGCCTTTGA

Coding sequence (CDS)

ATGACGAAGTCCTGTGTTATTTCACGAGTGAGTGAGCAAAGTCGTAACTTTGTGAACTTGCACGATTTCTTGAAAACGAAGAGAGGCATTGTGAAGTATGCAAAAAAGACACTGCCCGACAACAACTGGAACGACAGCGTCAAACATTACAATGGAAGATTTGCTAATTTGACCGTCGAAGAATCCCACGGTTCGTGTTGGATTGGAGATCGTCTTCTTCGTAAAAGGGGCTCTGGCCTTGTTTTCTTGGCCAAGAACAGAGAACCCACCAACCCAAATTCAGATCTTCCCACAGAAATCGTTGTCAAATCTTGTCTCATGGAATTCTCTTCTTCACTTCAACACGAGAAGGAAGTGCTCAGTGATTTGGCACCCTGCACTAATATAGTGCATTGCTACGGCGATGAAATCACAACGACAAAATCCGGTGAGAAAGTTTACAACGTCCTGTTCGAGTATTGCTCTGGCTCGAGCTTGCGTGACCACATTATTAACTTTGGACTTAATGGATTGCCGGAATACGAAGTTCGTAAGTATACAAGAGACATTGTTCATGGGCTCTATCACATGCATTGCAATGCTCGCTACGTCCACGGTGACATAAAACCAAGAAACATCTTATTGTTACCTAGTGCTTGCGCAGCTGACAAAGGTTCTCTAGTGGCTAAGATGGCCAGTTTTGGATTGGCTAAGAAGCTAACTCCTGAAGTAGTATGCGATCAAGAAATGAGTGGTTCGGGACCGTACATATCTCCAGAGTTGGCAAGGGATGGCTATTTAGATTGGCCTGCTGACATTTGGGCGCTTGGATGCGTGGTGTTGGAGATGTTGACTGGAAAACCAGCATGGAGCTTTGAGAATGCTAATAAGTACATGTTGGATTGCTACAACAATGAGCTGCTGCCAGAGATTCCGAAGGGGATGTCAAGTGCTGGAAGAGATTTTATAAAAAGGTGTTTGATTAGAAGTCCTTACAAAAGAAGACCGATTTGGTTGTTGATCAAACATCCTTTTGTTTGCCTTTGA

Protein sequence

MTKSCVISRVSEQSRNFVNLHDFLKTKRGIVKYAKKTLPDNNWNDSVKHYNGRFANLTVEESHGSCWIGDRLLRKRGSGLVFLAKNREPTNPNSDLPTEIVVKSCLMEFSSSLQHEKEVLSDLAPCTNIVHCYGDEITTTKSGEKVYNVLFEYCSGSSLRDHIINFGLNGLPEYEVRKYTRDIVHGLYHMHCNARYVHGDIKPRNILLLPSACAADKGSLVAKMASFGLAKKLTPEVVCDQEMSGSGPYISPELARDGYLDWPADIWALGCVVLEMLTGKPAWSFENANKYMLDCYNNELLPEIPKGMSSAGRDFIKRCLIRSPYKRRPIWLLIKHPFVCL

Homology

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BLAST of Spg022631 vs. NCBI nr
Match: XP_023516554.1 (mitogen-activated protein kinase kinase kinase 17-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 525.4 bits (1352), Expect = 3.7e-145
Identity = 248/339 (73.16%), Postives = 285/339 (84.07%), Query Frame = 0


Query: 1   MTKSCVISRVSEQSRNFVNLHDFLKTKRGIVKYAKKTLPDNNWNDSVKHYNGRFANLTVE 60
           MTKSCV+S+V++Q  N VNLH+FLKTKRGIVK AKK+   NNWN++VKH  GRF N  +E
Sbjct: 1   MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSNNWNNNVKHNYGRFGNFAIE 60

Query: 61  ESHGSCWIGDRLLRKRGSGLVFLAKNREPTNPNSDLPTEIVVKSCLMEFSSSLQHEKEVL 120
           E HGS WIGDRL+RK GSGLVFLAKN+  TN N     E +VKSCLME SSSLQ+EKEVL
Sbjct: 61  EYHGSFWIGDRLIRKSGSGLVFLAKNKSSTNSN----PEFLVKSCLMESSSSLQYEKEVL 120

Query: 121 SDLAPCTNIVHCYGDEITTTKSGEKVYNVLFEYCSGSSLRDHIINFGLNGLPEYEVRKYT 180
            +L PC+N+V CYGDEIT  KSGE++YNV+FEYC GSSLRDHII FG NGLPE  VR+YT
Sbjct: 121 RNLGPCSNLVRCYGDEITMNKSGEQIYNVVFEYCRGSSLRDHIIKFGRNGLPEPIVRQYT 180

Query: 181 RDIVHGLYHMHCNARYVHGDIKPRNILLLPSACAADKGSLVAKMASFGLAKKLTPEVVCD 240
           RDIVHGL +MHCNA Y+HGDIKPRNILL PS      G LVAK+ASFGLA+KLTPEV+CD
Sbjct: 181 RDIVHGLCYMHCNAGYIHGDIKPRNILLSPS--DHQGGCLVAKLASFGLARKLTPEVLCD 240

Query: 241 QEMSGSGPYISPELARDGYLDWPADIWALGCVVLEMLTGKPAWSFENANKYMLDCYNNEL 300
           +E+SGSG YI+PELA+DGYLDWPADIWALGCVVLEMLTGKPAWSFE+A  Y++DC  N+ 
Sbjct: 241 EEISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMDC--NQE 300

Query: 301 LPEIPKGMSSAGRDFIKRCLIRSPYKRRPIWLLIKHPFV 340
           +PEIP  +SSAGRDF+KRCLIRSPYKRRP WLL+KHPFV
Sbjct: 301 IPEIPSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFV 331

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BLAST of Spg022631 vs. NCBI nr
Match: KAG7023508.1 (Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 521.2 bits (1341), Expect = 6.9e-144
Identity = 247/339 (72.86%), Postives = 284/339 (83.78%), Query Frame = 0


Query: 1   MTKSCVISRVSEQSRNFVNLHDFLKTKRGIVKYAKKTLPDNNWNDSVKHYNGRFANLTVE 60
           MTKSCV+S+V++Q  N VNLH+FLKTKRGIVK AKK+    NWN++VKH  GRF N  +E
Sbjct: 1   MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSTNWNNNVKHNYGRFGNFAIE 60

Query: 61  ESHGSCWIGDRLLRKRGSGLVFLAKNREPTNPNSDLPTEIVVKSCLMEFSSSLQHEKEVL 120
           E HGS WIGDRL+RK GSGLVFLAKN+  TN NS    E +VKSCLME SSSLQ+EKEVL
Sbjct: 61  EYHGSFWIGDRLIRKSGSGLVFLAKNKSSTNSNS----EFLVKSCLMESSSSLQYEKEVL 120

Query: 121 SDLAPCTNIVHCYGDEITTTKSGEKVYNVLFEYCSGSSLRDHIINFGLNGLPEYEVRKYT 180
            +L PC+N+V CYGDEIT  KSGE++YNV+FEYC GSSLRDHII FG NGLPE  VRKYT
Sbjct: 121 CNLGPCSNLVRCYGDEITMNKSGEQIYNVVFEYCCGSSLRDHIIKFGRNGLPEPIVRKYT 180

Query: 181 RDIVHGLYHMHCNARYVHGDIKPRNILLLPSACAADKGSLVAKMASFGLAKKLTPEVVCD 240
           RDIV GL +MHCNA Y+HGDIKPRNILL PS      G LVAK+ASFGLA+KLTPEV+CD
Sbjct: 181 RDIVRGLCYMHCNAGYIHGDIKPRNILLSPSDHKG--GCLVAKLASFGLARKLTPEVLCD 240

Query: 241 QEMSGSGPYISPELARDGYLDWPADIWALGCVVLEMLTGKPAWSFENANKYMLDCYNNEL 300
           +E+SGSG YI+PELA+DGYLDWPADIWALGCVVLEMLTGKPAWSFE+A  Y+++C  N+ 
Sbjct: 241 EEISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMNC--NQE 300

Query: 301 LPEIPKGMSSAGRDFIKRCLIRSPYKRRPIWLLIKHPFV 340
           +PEIP  +SSAGRDF+KRCLIRSPYKRRP WLL+KHPFV
Sbjct: 301 IPEIPSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFV 331

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BLAST of Spg022631 vs. NCBI nr
Match: XP_022960664.1 (mitogen-activated protein kinase kinase kinase 17-like [Cucurbita moschata])

HSP 1 Score: 520.8 bits (1340), Expect = 9.0e-144
Identity = 246/339 (72.57%), Postives = 285/339 (84.07%), Query Frame = 0


Query: 1   MTKSCVISRVSEQSRNFVNLHDFLKTKRGIVKYAKKTLPDNNWNDSVKHYNGRFANLTVE 60
           MTKSCV+S+V++Q  N VNLH+FLKTKRGIVK AKK+    NWN++VKH  GRF N  +E
Sbjct: 1   MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSTNWNNNVKHNYGRFGNFAIE 60

Query: 61  ESHGSCWIGDRLLRKRGSGLVFLAKNREPTNPNSDLPTEIVVKSCLMEFSSSLQHEKEVL 120
           E HGS WIGDRL+R+ GSGLVFLAKN+  TN NS    E +VKSCLME SSSLQ+EKEVL
Sbjct: 61  ECHGSFWIGDRLIRRSGSGLVFLAKNKSSTNSNS----EFLVKSCLMESSSSLQYEKEVL 120

Query: 121 SDLAPCTNIVHCYGDEITTTKSGEKVYNVLFEYCSGSSLRDHIINFGLNGLPEYEVRKYT 180
            +L PC+N+V CYGDEIT +KSGE++YNV+FEYC GSSLRDHII FG NGLPE  VRKYT
Sbjct: 121 CNLGPCSNLVRCYGDEITMSKSGEQIYNVVFEYCCGSSLRDHIIKFGRNGLPEPIVRKYT 180

Query: 181 RDIVHGLYHMHCNARYVHGDIKPRNILLLPSACAADKGSLVAKMASFGLAKKLTPEVVCD 240
           RDIV GL +MHCNA Y+HGDIKPRNILL PS      G LVAK+ASFGLA+KLTPEV+CD
Sbjct: 181 RDIVRGLCYMHCNAGYIHGDIKPRNILLSPSDHKG--GCLVAKLASFGLARKLTPEVLCD 240

Query: 241 QEMSGSGPYISPELARDGYLDWPADIWALGCVVLEMLTGKPAWSFENANKYMLDCYNNEL 300
           +E+SGSG YI+PELA+DGYLDWPADIWALGCVVLEMLTGKPAWSFE+A  Y+++C  N+ 
Sbjct: 241 EEISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMNC--NQE 300

Query: 301 LPEIPKGMSSAGRDFIKRCLIRSPYKRRPIWLLIKHPFV 340
           +PEIP  +SSAGRDF+KRCLIRSPYKRRP WLL+KHPFV
Sbjct: 301 IPEIPSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFV 331

[Close]

BLAST of Spg022631 vs. NCBI nr
Match: KAG6589837.1 (Mitogen-activated protein kinase kinase kinase 20, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 520.4 bits (1339), Expect = 1.2e-143
Identity = 246/339 (72.57%), Postives = 284/339 (83.78%), Query Frame = 0


Query: 1   MTKSCVISRVSEQSRNFVNLHDFLKTKRGIVKYAKKTLPDNNWNDSVKHYNGRFANLTVE 60
           MTKSCV+S+V++Q  N VNLH+FLKTKRGIVK AKK+    NWN++VKH  GRF N  +E
Sbjct: 1   MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSTNWNNNVKHNYGRFGNFAIE 60

Query: 61  ESHGSCWIGDRLLRKRGSGLVFLAKNREPTNPNSDLPTEIVVKSCLMEFSSSLQHEKEVL 120
           E HGS WIGDRL+RK GSGLVFLAKN+  TN NS    E +VKSCLME SSSLQ+EKEVL
Sbjct: 61  ECHGSFWIGDRLIRKSGSGLVFLAKNKSSTNSNS----EFLVKSCLMESSSSLQYEKEVL 120

Query: 121 SDLAPCTNIVHCYGDEITTTKSGEKVYNVLFEYCSGSSLRDHIINFGLNGLPEYEVRKYT 180
            +L PC+N+V CYGDEIT  KSGE++YNV+FEYC GSSLRDHII FG NGLPE  VRKYT
Sbjct: 121 CNLGPCSNLVRCYGDEITMNKSGEQIYNVVFEYCCGSSLRDHIIKFGRNGLPEPIVRKYT 180

Query: 181 RDIVHGLYHMHCNARYVHGDIKPRNILLLPSACAADKGSLVAKMASFGLAKKLTPEVVCD 240
           RDIV GL ++HCNA Y+HGDIKPRNILL PS      G LVAK+ASFGLA+KLTPEV+CD
Sbjct: 181 RDIVRGLCYIHCNAGYIHGDIKPRNILLSPS--DRKGGCLVAKLASFGLARKLTPEVLCD 240

Query: 241 QEMSGSGPYISPELARDGYLDWPADIWALGCVVLEMLTGKPAWSFENANKYMLDCYNNEL 300
           +E+SGSG YI+PELA+DGYLDWPADIWALGCVVLEMLTGKPAWSFE+A  Y+++C  N+ 
Sbjct: 241 EEISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMNC--NQE 300

Query: 301 LPEIPKGMSSAGRDFIKRCLIRSPYKRRPIWLLIKHPFV 340
           +PEIP  +SSAGRDF+KRCLIRSPYKRRP WLL+KHPFV
Sbjct: 301 IPEIPSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFV 331

[Close]

BLAST of Spg022631 vs. NCBI nr
Match: XP_038880300.1 (mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida])

HSP 1 Score: 479.2 bits (1232), Expect = 3.0e-131
Identity = 231/342 (67.54%), Postives = 269/342 (78.65%), Query Frame = 0


Query: 1   MTKSCVISRVSEQSRNFVNLHDFLKTKRGIVKYAKKTLPDNNWNDSVKHYNGRFA-NLTV 60
           MTKSCV+S+V+++S NFVNLH+FLKTKRGIVK AKK+L  NNWND+VKH + +F  +  +
Sbjct: 1   MTKSCVVSQVNQRSHNFVNLHEFLKTKRGIVKCAKKSLRTNNWNDNVKHNSEKFGKDFAI 60

Query: 61  EESHGSCWIGDRLLRKRGSGLVFLAKNREPTNPNSDLPTEIVVKSCLMEFSSSLQHEKEV 120
           EE HGS W GD L RK GS L+FLA+NR PTNPNS+LPTE +VKSCLME SSSLQHEKEV
Sbjct: 61  EEYHGSIWTGDHLFRKTGSSLIFLARNRAPTNPNSNLPTEFMVKSCLMESSSSLQHEKEV 120

Query: 121 LSDLAPCTNIVHCYGDEITTTKSGEKVYNVLFEYCSGSSLRDHIINFGLNGLPEYEVRKY 180
           L++L   TN+V CYGDEIT TKSGEKVYNVLFEYC GSSLRDHI+ FG NGLPE+EVR+Y
Sbjct: 121 LNNLGSFTNLVRCYGDEITITKSGEKVYNVLFEYCYGSSLRDHILKFGPNGLPEHEVRRY 180

Query: 181 TRDIVHGLYHMHCNARYVHGDIKPRNILLLPSACAADKGSLVAKMASFGLAKKLTPEVVC 240
           TRDIV+G+ +MHCN RY+HGDIK RNIL+ P+          AK+ASFG A+KLT EV+C
Sbjct: 181 TRDIVYGVCYMHCNGRYIHGDIKSRNILMSPTG---------AKLASFGWARKLTAEVLC 240

Query: 241 DQEMSGSGPYISPELARDGYLDWPADIWALGCVVLEMLTGKPAWSFENANKYMLDCYNNE 300
           ++E+ GSGPYISPELA DGYL WPADIWALGCVVLEMLTGK                NNE
Sbjct: 241 EEEIRGSGPYISPELASDGYLGWPADIWALGCVVLEMLTGKSP-------------RNNE 300

Query: 301 LLPEIPKGMSSAGRDFIKRCLIRSPYKRRPIWLLIKHPFVCL 342
              +I   +S  GRDFIK+CLI SPYKRRP WLLIKHPFVC+
Sbjct: 301 EAIQIINDISKEGRDFIKKCLITSPYKRRPSWLLIKHPFVCV 320

[Close]

BLAST of Spg022631 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 5.5e-43
Identity = 96/271 (35.42%), Postives = 149/271 (54.98%), Query Frame = 0


Query: 77  GSGLVFLAKNREPTNPNSDLPTEIVVKSCLMEFSSSLQHEKEVLSDLAPCTNIVHCYGDE 136
           G G          +  + D P  I VKS     ++SL +EK VL  L  C  I+ CYG++
Sbjct: 10  GFGTFSTVSTATKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCPEIIRCYGED 69

Query: 137 ITTTKSGEKVYNVLFEYCSGSSLRDHIINFGLNGLPEYEVRKYTRDIVHGLYHMHCNARY 196
            +T ++GE+++N+L EY S  SL  ++   G  GLPE  VR++T  ++ GL H+H    +
Sbjct: 70  -STVENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGLRHIHAKG-F 129

Query: 197 VHGDIKPRNILLLPSACAADKGSLVAKMASFGLAKKLTPEVVC---DQEMSGSGPYISPE 256
            H DIK  NILL       + GS+  K+A FGLA ++  ++       E+ G+  Y++PE
Sbjct: 130 AHCDIKLANILLF------NDGSV--KIADFGLAMRVDGDLTALRKSVEIRGTPLYMAPE 189

Query: 257 LARDGYLDWPADIWALGCVVLEMLTGKPAWSFENANKYM---LDCYNNELLPEIPKGMSS 316
              D      AD+WALGC V+EM +GK AWS +  + +M   +     + LP+IP+ +S 
Sbjct: 190 CVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEMLSE 249

Query: 317 AGRDFIKRCLIRSPYKRRPIWLLIKHPFVCL 342
            G+DF+ +C ++ P KR    +L+ H FV +
Sbjct: 250 EGKDFLSKCFVKDPAKRWTAEMLLNHSFVTI 270

[Close]

BLAST of Spg022631 vs. ExPASy Swiss-Prot
Match: Q9CAD5 (Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3702 GN=YDA PE=1 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 5.9e-29
Identity = 98/284 (34.51%), Postives = 137/284 (48.24%), Query Frame = 0


Query: 62  SHGSCWIGDRLLRKRGSGLVFLAKNREPTNPNSDLPTEIVVKSC-----LMEFSSSLQHE 121
           S GS W   RLL     G V+L  N E    + ++     V  C       E +  L  E
Sbjct: 395 SPGSRWKKGRLLGMGSFGHVYLGFNSE----SGEMCAMKEVTLCSDDPKSRESAQQLGQE 454

Query: 122 KEVLSDLAPCTNIVHCYGDEITTTKSGEKVYNVLFEYCSGSSLRDHIINFGLNGLPEYEV 181
             VLS L    NIV  YG E       +K+Y +  EY SG S+   +  +G  G  E  +
Sbjct: 455 ISVLSRLRH-QNIVQYYGSETV----DDKLY-IYLEYVSGGSIYKLLQEYGQFG--ENAI 514

Query: 182 RKYTRDIVHGLYHMHCNARYVHGDIKPRNILLLPSACAADKGSLVAKMASFGLAKKLTPE 241
           R YT+ I+ GL ++H     VH DIK  NIL+ P            K+A FG+AK +T +
Sbjct: 515 RNYTQQILSGLAYLHAK-NTVHRDIKGANILVDPHG--------RVKVADFGMAKHITAQ 574

Query: 242 VVCDQEMSGSGPYISPELARDGY-LDWPADIWALGCVVLEMLTGKPAWSFENANKYMLDC 301
                   GS  +++PE+ ++    +   DIW+LGC VLEM T KP WS       M   
Sbjct: 575 -SGPLSFKGSPYWMAPEVIKNSNGSNLAVDIWSLGCTVLEMATTKPPWSQYEGVPAMFKI 634

Query: 302 YNNELLPEIPKGMSSAGRDFIKRCLIRSPYKRRPIWLLIKHPFV 340
            N++ LP+IP  +S  G+DF+++CL R+P  R     L+ H FV
Sbjct: 635 GNSKELPDIPDHLSEEGKDFVRKCLQRNPANRPTAAQLLDHAFV 656

[Close]

BLAST of Spg022631 vs. ExPASy Swiss-Prot
Match: Q54R82 (Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=44689 GN=mkkA PE=1 SV=2)

HSP 1 Score: 128.3 bits (321), Expect = 1.7e-28
Identity = 90/314 (28.66%), Postives = 149/314 (47.45%), Query Frame = 0


Query: 40  DNNWNDSVKHYNGRFANLTVEE------SHGSCWIGDRLLRKRGSGLVFLAKNREPTNPN 99
           +NN N+ ++H +  F +L + E      +H   W   ++L + G G V+L  N++     
Sbjct: 139 NNNNNNIIQHTD--FPSLIINEHEELISNHNIKWQKGQILGRGGYGSVYLGLNKDTGELF 198

Query: 100 SDLPTEIV-------VKSCLMEFSSSLQHEKEVLSDLAPCTNIVHCYGDEITTTKSGEKV 159
           +    EIV       +K+ ++ FS  ++  + +  D     NIV   G     T   +  
Sbjct: 199 AVKQLEIVDINSDPKLKNMILSFSKEIEVMRSLRHD-----NIVRYLG-----TSLDQSF 258

Query: 160 YNVLFEYCSGSSLRDHIINFGLNGLPEYEVRKYTRDIVHGLYHMHCNARYVHGDIKPRNI 219
            +V  EY  G S+   +  FG     E  ++ YT+ I+ GL  +H N+  +H DIK  NI
Sbjct: 259 LSVFLEYIPGGSISSLLGKFG--AFSENVIKVYTKQILQGLSFLHANS-IIHRDIKGANI 318

Query: 220 LLLPSACAADKGSLVAKMASFGLAKKLTPEVVCDQEMSGSGPYISPELARDGYLDWPADI 279
           L+        KG  + K++ FG +K  +  V   + M G+  +++PE+ +       +DI
Sbjct: 319 LI------DTKG--IVKLSDFGCSKSFSGIVSQFKSMQGTPYWMAPEVIKQTGHGRSSDI 378

Query: 280 WALGCVVLEMLTGKPAWS-FENANKYMLDCYNNELLPEIPKGMSSAGRDFIKRCLIRSPY 339
           W+LGCV++EM T +P WS        M    ++  +P IP  MS    DF+  C  R P 
Sbjct: 379 WSLGCVIVEMATAQPPWSNITELAAVMYHIASSNSIPNIPSHMSQEAFDFLNLCFKRDPK 429

[Close]

BLAST of Spg022631 vs. ExPASy Swiss-Prot
Match: F4HRJ4 (Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK3 PE=1 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 3.2e-27
Identity = 90/282 (31.91%), Postives = 138/282 (48.94%), Query Frame = 0


Query: 65  SCWIGDRLLRKRGSGLVFLAKNREPTNPNSDLPTEIV-----VKSCLMEFSSSLQHEKEV 124
           S W   + L     G V+L  N E     +    +++      K CL +    L  E  +
Sbjct: 212 STWKKGKFLGSGTFGQVYLGFNSEKGKMCAIKEVKVISDDQTSKECLKQ----LNQEINL 271

Query: 125 LSDLAPCTNIVHCYGDEITTTKSGEKVYNVLFEYCSGSSLRDHIINFGLNGLPEYEVRKY 184
           L+ L    NIV  YG E++     E+  +V  EY SG S+   + ++G     E  ++ Y
Sbjct: 272 LNQLCH-PNIVQYYGSELS-----EETLSVYLEYVSGGSIHKLLKDYG--SFTEPVIQNY 331

Query: 185 TRDIVHGLYHMHCNARYVHGDIKPRNILLLPSACAADKGSLVAKMASFGLAKKLTPEVVC 244
           TR I+ GL ++H     VH DIK  NIL+ P+           K+A FG+AK +T     
Sbjct: 332 TRQILAGLAYLH-GRNTVHRDIKGANILVDPNG--------EIKLADFGMAKHVTAFSTM 391

Query: 245 DQEMSGSGPYISPE--LARDGYLDWPADIWALGCVVLEMLTGKPAWSFENANKYMLDCYN 304
                GS  +++PE  ++++GY     DIW+LGC +LEM T KP WS       +    N
Sbjct: 392 -LSFKGSPYWMAPEVVMSQNGYTH-AVDIWSLGCTILEMATSKPPWSQFEGVAAIFKIGN 451

Query: 305 NELLPEIPKGMSSAGRDFIKRCLIRSPYKRRPIWLLIKHPFV 340
           ++  PEIP  +S+  ++FI+ CL R+P  R     L++HPF+
Sbjct: 452 SKDTPEIPDHLSNDAKNFIRLCLQRNPTVRPTASQLLEHPFL 470

[Close]

BLAST of Spg022631 vs. ExPASy Swiss-Prot
Match: Q61084 (Mitogen-activated protein kinase kinase kinase 3 OS=Mus musculus OX=10090 GN=Map3k3 PE=1 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 3.9e-25
Identity = 84/263 (31.94%), Postives = 124/263 (47.15%), Query Frame = 0


Query: 83  LAKNREPTNPNS-DLPTEIVVKSCLMEFSSSLQHEKEVLSDLAPCTNIVHCYGDEITTTK 142
           LA  +   +P+S +   E+    C ++   +LQHE+           IV  YG       
Sbjct: 388 LASKQVQFDPDSPETSKEVSALECEIQLLKNLQHER-----------IVQYYG---CLRD 447

Query: 143 SGEKVYNVLFEYCSGSSLRDHIINFGLNGLPEYEVRKYTRDIVHGLYHMHCNARYVHGDI 202
             EK+  +  EY  G S++D +  +G   L E   RKYTR I+ G+ ++H N   VH DI
Sbjct: 448 RAEKILTIFMEYMPGGSVKDQLKAYG--ALTESVTRKYTRQILEGMSYLHSN-MIVHRDI 507

Query: 203 KPRNILLLPSACAADKGSLVAKMASFGLAKKLTPEVVCDQEMSGSG-------PY-ISPE 262
           K  NIL        D    V K+  FG +K+L  + +C   MSG+G       PY +SPE
Sbjct: 508 KGANIL-------RDSAGNV-KLGDFGASKRL--QTIC---MSGTGIRSVTGTPYWMSPE 567

Query: 263 LARDGYLDWPADIWALGCVVLEMLTGKPAWSFENANKYMLDCYNNELLPEIPKGMSSAGR 322
           +         AD+W+LGC V+EMLT KP W+   A   +         P++P  +S  GR
Sbjct: 568 VISGEGYGRKADVWSLGCTVVEMLTEKPPWAEYEAMAAIFKIATQPTNPQLPSHISEHGR 620

Query: 323 DFIKRCLIRSPYKRRPIWLLIKH 337
           DF++R  + +  +     LL  H
Sbjct: 628 DFLRRIFVEARQRPSAEELLTHH 620

[Close]

BLAST of Spg022631 vs. ExPASy TrEMBL
Match: A0A6J1HBP5 (mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=3662 GN=LOC111461386 PE=4 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 4.4e-144
Identity = 246/339 (72.57%), Postives = 285/339 (84.07%), Query Frame = 0


Query: 1   MTKSCVISRVSEQSRNFVNLHDFLKTKRGIVKYAKKTLPDNNWNDSVKHYNGRFANLTVE 60
           MTKSCV+S+V++Q  N VNLH+FLKTKRGIVK AKK+    NWN++VKH  GRF N  +E
Sbjct: 1   MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSTNWNNNVKHNYGRFGNFAIE 60

Query: 61  ESHGSCWIGDRLLRKRGSGLVFLAKNREPTNPNSDLPTEIVVKSCLMEFSSSLQHEKEVL 120
           E HGS WIGDRL+R+ GSGLVFLAKN+  TN NS    E +VKSCLME SSSLQ+EKEVL
Sbjct: 61  ECHGSFWIGDRLIRRSGSGLVFLAKNKSSTNSNS----EFLVKSCLMESSSSLQYEKEVL 120

Query: 121 SDLAPCTNIVHCYGDEITTTKSGEKVYNVLFEYCSGSSLRDHIINFGLNGLPEYEVRKYT 180
            +L PC+N+V CYGDEIT +KSGE++YNV+FEYC GSSLRDHII FG NGLPE  VRKYT
Sbjct: 121 CNLGPCSNLVRCYGDEITMSKSGEQIYNVVFEYCCGSSLRDHIIKFGRNGLPEPIVRKYT 180

Query: 181 RDIVHGLYHMHCNARYVHGDIKPRNILLLPSACAADKGSLVAKMASFGLAKKLTPEVVCD 240
           RDIV GL +MHCNA Y+HGDIKPRNILL PS      G LVAK+ASFGLA+KLTPEV+CD
Sbjct: 181 RDIVRGLCYMHCNAGYIHGDIKPRNILLSPSDHKG--GCLVAKLASFGLARKLTPEVLCD 240

Query: 241 QEMSGSGPYISPELARDGYLDWPADIWALGCVVLEMLTGKPAWSFENANKYMLDCYNNEL 300
           +E+SGSG YI+PELA+DGYLDWPADIWALGCVVLEMLTGKPAWSFE+A  Y+++C  N+ 
Sbjct: 241 EEISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMNC--NQE 300

Query: 301 LPEIPKGMSSAGRDFIKRCLIRSPYKRRPIWLLIKHPFV 340
           +PEIP  +SSAGRDF+KRCLIRSPYKRRP WLL+KHPFV
Sbjct: 301 IPEIPSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFV 331

[Close]

BLAST of Spg022631 vs. ExPASy TrEMBL
Match: A0A5D3D3K9 (Mitogen-activated protein kinase kinase kinase YODA-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1804G00170 PE=4 SV=1)

HSP 1 Score: 476.5 bits (1225), Expect = 9.4e-131
Identity = 235/343 (68.51%), Postives = 270/343 (78.72%), Query Frame = 0


Query: 1   MTKSCVISRVSEQSRNFVNLHDFLKTKRGIVKYAKKTLPDNNWNDSVKHYNGRFA-NLTV 60
           MTKSCV+SRV    +  VNLH+FLKTKR I+K AKK+ P NNWND++KH   +F  + T+
Sbjct: 1   MTKSCVVSRV----QFCVNLHEFLKTKRRILKIAKKSAPTNNWNDTIKHNYEKFGEDFTI 60

Query: 61  EESHGSCWIGDRLLRKRGSGLVFLA-KNREP-TNPNSDLPTEIVVKSCLMEFSSSLQHEK 120
           EE HGS W G  L RK  S L+FLA KNR P  N  S+LP E +VKS L E SSSL HEK
Sbjct: 61  EEFHGSIWSGVHLFRKTESTLIFLAKKNRAPINNSTSNLPEEFLVKSSLTESSSSLHHEK 120

Query: 121 EVLSDLAPCTNIVHCYGDEITTTKSGEKVYNVLFEYCSGSSLRDHIINFGLNGLPEYEVR 180
           EVLS+L P  N+V CYGDEIT +KSGEKVYNV FEYCSGSSLRDHI  FG NGL E+EVR
Sbjct: 121 EVLSNLGPFMNLVDCYGDEITISKSGEKVYNVFFEYCSGSSLRDHIRKFGPNGLQEHEVR 180

Query: 181 KYTRDIVHGLYHMHCNARYVHGDIKPRNILLLPSACAADKGSLVAKMASFGLAKKLTPEV 240
           KYTRDIVHGLY+MHCN RY+HGDIK RNILL            +AK+ASFGLA+KLT EV
Sbjct: 181 KYTRDIVHGLYYMHCNGRYIHGDIKSRNILLSHG---------MAKLASFGLARKLTAEV 240

Query: 241 VCDQEMSGSGPYISPELARDGYLDWPADIWALGCVVLEMLTGKPAWSFENANKYMLDCYN 300
           +C++E+SGSGPY SPELAR+GYL WPADIWALGCVVLEM TGK AWSF +A++Y++DC N
Sbjct: 241 ICEEEISGSGPYASPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFGDAHRYLMDC-N 300

Query: 301 NELLPEIPKGMSSAGRDFIKRCLIRSPYKRRPIWLLIKHPFVC 341
           NE +PEIPK +S  GRDFIK+CLIRSPYKRRP WLLIKHPFVC
Sbjct: 301 NEKIPEIPKNISREGRDFIKKCLIRSPYKRRPSWLLIKHPFVC 329

[Close]

BLAST of Spg022631 vs. ExPASy TrEMBL
Match: A0A1S3B829 (mitogen-activated protein kinase kinase kinase YODA-like OS=Cucumis melo OX=3656 GN=LOC103487049 PE=4 SV=1)

HSP 1 Score: 476.5 bits (1225), Expect = 9.4e-131
Identity = 235/343 (68.51%), Postives = 270/343 (78.72%), Query Frame = 0


Query: 1   MTKSCVISRVSEQSRNFVNLHDFLKTKRGIVKYAKKTLPDNNWNDSVKHYNGRFA-NLTV 60
           MTKSCV+SRV    +  VNLH+FLKTKR I+K AKK+ P NNWND++KH   +F  + T+
Sbjct: 1   MTKSCVVSRV----QFCVNLHEFLKTKRRILKIAKKSAPTNNWNDTIKHNYEKFGEDFTI 60

Query: 61  EESHGSCWIGDRLLRKRGSGLVFLA-KNREP-TNPNSDLPTEIVVKSCLMEFSSSLQHEK 120
           EE HGS W G  L RK  S L+FLA KNR P  N  S+LP E +VKS L E SSSL HEK
Sbjct: 61  EEFHGSIWSGVHLFRKTESTLIFLAKKNRAPINNSTSNLPEEFLVKSSLTESSSSLHHEK 120

Query: 121 EVLSDLAPCTNIVHCYGDEITTTKSGEKVYNVLFEYCSGSSLRDHIINFGLNGLPEYEVR 180
           EVLS+L P  N+V CYGDEIT +KSGEKVYNV FEYCSGSSLRDHI  FG NGL E+EVR
Sbjct: 121 EVLSNLGPFMNLVDCYGDEITISKSGEKVYNVFFEYCSGSSLRDHIRKFGPNGLQEHEVR 180

Query: 181 KYTRDIVHGLYHMHCNARYVHGDIKPRNILLLPSACAADKGSLVAKMASFGLAKKLTPEV 240
           KYTRDIVHGLY+MHCN RY+HGDIK RNILL            +AK+ASFGLA+KLT EV
Sbjct: 181 KYTRDIVHGLYYMHCNGRYIHGDIKSRNILLSHG---------MAKLASFGLARKLTAEV 240

Query: 241 VCDQEMSGSGPYISPELARDGYLDWPADIWALGCVVLEMLTGKPAWSFENANKYMLDCYN 300
           +C++E+SGSGPY SPELAR+GYL WPADIWALGCVVLEM TGK AWSF +A++Y++DC N
Sbjct: 241 ICEEEISGSGPYASPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFGDAHRYLMDC-N 300

Query: 301 NELLPEIPKGMSSAGRDFIKRCLIRSPYKRRPIWLLIKHPFVC 341
           NE +PEIPK +S  GRDFIK+CLIRSPYKRRP WLLIKHPFVC
Sbjct: 301 NEKIPEIPKNISREGRDFIKKCLIRSPYKRRPSWLLIKHPFVC 329

[Close]

BLAST of Spg022631 vs. ExPASy TrEMBL
Match: A0A0A0LHV1 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G829110 PE=4 SV=1)

HSP 1 Score: 475.3 bits (1222), Expect = 2.1e-130
Identity = 234/343 (68.22%), Postives = 271/343 (79.01%), Query Frame = 0


Query: 1   MTKSCVISRVSEQSRNFVNLHDFLKTKRGIVKYAKKTLPDNNWNDSVKHYNGRFA-NLTV 60
           MTKSCV+S+V        NLH+FLKTKR I+K AKK+ P NNWND++KH   +F  + T+
Sbjct: 1   MTKSCVVSQVQISG----NLHEFLKTKRRILKIAKKSAPRNNWNDTIKHNYEKFGEDFTI 60

Query: 61  EESHGSCWIGDRLLRKRGSGLVFLA-KNREP-TNPNSDLPTEIVVKSCLMEFSSSLQHEK 120
           EE HGS W G  L RK  S LVFLA KNR P  N NS+LP E +VKS LME SSSL+HEK
Sbjct: 61  EEFHGSIWTGSLLFRKTESTLVFLAKKNRAPINNSNSNLPEEFLVKSSLMESSSSLRHEK 120

Query: 121 EVLSDLAPCTNIVHCYGDEITTTKSGEKVYNVLFEYCSGSSLRDHIINFGLNGLPEYEVR 180
           EVLS+L P TN+V CYGDEIT TKSGE+VYNV FEYCSGSSLR+HI+ FG NGL + EVR
Sbjct: 121 EVLSNLGPFTNLVDCYGDEITVTKSGEEVYNVFFEYCSGSSLRNHILKFGPNGLQDDEVR 180

Query: 181 KYTRDIVHGLYHMHCNARYVHGDIKPRNILLLPSACAADKGSLVAKMASFGLAKKLTPEV 240
           +YTRDIV GLY+MHCN RY+HGDIK RNILL            +AK+ASFGLA+KLT EV
Sbjct: 181 RYTRDIVRGLYYMHCNGRYIHGDIKSRNILLSHG---------MAKLASFGLARKLTAEV 240

Query: 241 VCDQEMSGSGPYISPELARDGYLDWPADIWALGCVVLEMLTGKPAWSFENANKYMLDCYN 300
           +C++E+SGSGPY SPELAR+GYL WPADIWALGCVVLEM TGK AWSFE+A +Y++D  N
Sbjct: 241 ICEEEISGSGPYASPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFEDAYRYLMDS-N 300

Query: 301 NELLPEIPKGMSSAGRDFIKRCLIRSPYKRRPIWLLIKHPFVC 341
           NE +PEIPK +S  GRDFIK+CLIRSPYKRRPIWLLIKHPFVC
Sbjct: 301 NEKIPEIPKNISREGRDFIKKCLIRSPYKRRPIWLLIKHPFVC 329

[Close]

BLAST of Spg022631 vs. ExPASy TrEMBL
Match: A0A6J1JAH8 (mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita maxima OX=3661 GN=LOC111485031 PE=4 SV=1)

HSP 1 Score: 387.1 bits (993), Expect = 7.5e-104
Identity = 180/233 (77.25%), Postives = 204/233 (87.55%), Query Frame = 0


Query: 107 MEFSSSLQHEKEVLSDLAPCTNIVHCYGDEITTTKSGEKVYNVLFEYCSGSSLRDHIINF 166
           ME SSSLQ+EKEVL +L PC+N+V CYGDEIT  KSGE++YNV+FEYC GSSLRDHII F
Sbjct: 1   MESSSSLQYEKEVLCNLGPCSNLVRCYGDEITMNKSGEQIYNVVFEYCCGSSLRDHIIKF 60

Query: 167 GLNGLPEYEVRKYTRDIVHGLYHMHCNARYVHGDIKPRNILLLPSACAADKGSLVAKMAS 226
           G NGLPE  VRKYTRDIVHGL +MHCNARY+HGDIKPRNILL PS      G LVAK+AS
Sbjct: 61  GRNGLPEPIVRKYTRDIVHGLCYMHCNARYIHGDIKPRNILLSPSDHKG--GCLVAKLAS 120

Query: 227 FGLAKKLTPEVVCDQEMSGSGPYISPELARDGYLDWPADIWALGCVVLEMLTGKPAWSFE 286
           FGLA+KLTPEV+CD+E+SGSG YI+PELA+DGYLDWPADIWALGCVVLEMLTGKPAWSFE
Sbjct: 121 FGLARKLTPEVLCDEEISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFE 180

Query: 287 NANKYMLDCYNNELLPEIPKGMSSAGRDFIKRCLIRSPYKRRPIWLLIKHPFV 340
           +A  Y++DC  N+ +PEIP  +SSAGRDF+KRCLIRSPYKRRP WLL+KHPFV
Sbjct: 181 SAYNYLMDC--NQEIPEIPSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFV 229

[Close]

BLAST of Spg022631 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 182.2 bits (461), Expect = 7.1e-46
Identity = 98/250 (39.20%), Postives = 150/250 (60.00%), Query Frame = 0


Query: 97  PTEIVVKSCLMEFSSSLQHEKEVLSDLAPCTNIVHCYGDEITTTKSGEKVYNVLFEYCSG 156
           P+ + VKS  +  S++L++E++VL DL  C+ IV C+G E  T ++GE++YN+  EY SG
Sbjct: 31  PSLMAVKSSGVVCSAALRNERDVLDDLGDCSEIVRCFG-EGRTVENGEEIYNLFLEYASG 90

Query: 157 SSLRDHIINFGLNGLPEYEVRKYTRDIVHGLYHMHCNARYVHGDIKPRNILLLPSACAAD 216
            SL D I + G   LPE+EVR++TR IV GL H+H N  + H DIK  N+L+        
Sbjct: 91  GSLADRIKSSG-EALPEFEVRRFTRSIVKGLCHIHGNG-FTHCDIKLENVLVF------- 150

Query: 217 KGSLVAKMASFGLAKKLTPEVVCDQEMSGSGPYISPELARDGYLDWPADIWALGCVVLEM 276
            G    K++ FGLAK+ + EV    E+ G+  Y++PE    G  + PADIWALGC V+EM
Sbjct: 151 -GDGDVKISDFGLAKRRSGEVCV--EIRGTPLYMAPESVNHGEFESPADIWALGCSVVEM 210

Query: 277 LTGKPAWSFE-----NANKYMLDCYNNELLPEIPKGMSSAGRDFIKRCLIRSPYKRRPIW 336
            +GK AW  E     N    ++   + + +P IP  +S  G+DF+ +C +++  +R    
Sbjct: 211 SSGKTAWCLEDGVMNNVMSLLVRIGSGDEVPRIPVELSEEGKDFVSKCFVKNAAERWTAE 267

Query: 337 LLIKHPFVCL 342
           +L+ HPF+ +
Sbjct: 271 MLLDHPFLAV 267

[Close]

BLAST of Spg022631 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 176.4 bits (446), Expect = 3.9e-44
Identity = 96/271 (35.42%), Postives = 149/271 (54.98%), Query Frame = 0


Query: 77  GSGLVFLAKNREPTNPNSDLPTEIVVKSCLMEFSSSLQHEKEVLSDLAPCTNIVHCYGDE 136
           G G          +  + D P  I VKS     ++SL +EK VL  L  C  I+ CYG++
Sbjct: 10  GFGTFSTVSTATKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCPEIIRCYGED 69

Query: 137 ITTTKSGEKVYNVLFEYCSGSSLRDHIINFGLNGLPEYEVRKYTRDIVHGLYHMHCNARY 196
            +T ++GE+++N+L EY S  SL  ++   G  GLPE  VR++T  ++ GL H+H    +
Sbjct: 70  -STVENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGLRHIHAKG-F 129

Query: 197 VHGDIKPRNILLLPSACAADKGSLVAKMASFGLAKKLTPEVVC---DQEMSGSGPYISPE 256
            H DIK  NILL       + GS+  K+A FGLA ++  ++       E+ G+  Y++PE
Sbjct: 130 AHCDIKLANILLF------NDGSV--KIADFGLAMRVDGDLTALRKSVEIRGTPLYMAPE 189

Query: 257 LARDGYLDWPADIWALGCVVLEMLTGKPAWSFENANKYM---LDCYNNELLPEIPKGMSS 316
              D      AD+WALGC V+EM +GK AWS +  + +M   +     + LP+IP+ +S 
Sbjct: 190 CVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEMLSE 249

Query: 317 AGRDFIKRCLIRSPYKRRPIWLLIKHPFVCL 342
            G+DF+ +C ++ P KR    +L+ H FV +
Sbjct: 250 EGKDFLSKCFVKDPAKRWTAEMLLNHSFVTI 270

[Close]

BLAST of Spg022631 vs. TAIR 10
Match: AT3G46140.1 (Protein kinase superfamily protein )

HSP 1 Score: 172.6 bits (436), Expect = 5.6e-43
Identity = 116/319 (36.36%), Postives = 164/319 (51.41%), Query Frame = 0


Query: 35  KKTLPDNNWNDSVKHYNGRFANLTVEESHGSCWIGDRLLRKRGSGLVFLAKNREPTNPNS 94
           K+  P ++ + SV+    +  +  V +S  S WI    L +   G V+LA     T+  +
Sbjct: 66  KRFTPKSSSSRSVEEITKQVFDGVVRKS--SSWIKSEFLGRGSYGSVYLA-----TSKKA 125

Query: 95  DLPTEIVVKSCLMEFSSSLQHEKEVLSDLAPCTNIVHCYGDEIT---TTKSGEKV-YNVL 154
              T + +KS  +  +SSL  E+ +L+ L+    IV CYG EI    T   GE+  YN++
Sbjct: 126 KTKTTMAIKSAEISRASSLMDEERILTRLS-SPFIVRCYGHEIAREETLFGGERTNYNLI 185

Query: 155 FEYCSGSSLRDHIINFGLNGLPEYEVRKYTRDIVHGLYHMHCNARYVHGDIKPRNILLLP 214
            EYCSG SL D ++N  L GL E +V+   RDI++GL  +H  A  +H DIKP NI L P
Sbjct: 186 LEYCSGKSLFD-LVNDNLGGLSEKDVKLLARDILYGLDCIH-RANIIHCDIKPENIFLTP 245

Query: 215 SACAADKGSLVAKMASFGLA--KKLTPEVVCDQEMSGSGPYISPELARDGYLDWPADIWA 274
                     VAK+  FGLA  K  +          G+  Y+SPEL R G +D+  D WA
Sbjct: 246 VENRIRPSGYVAKIGDFGLALEKGSSEYEKASGHRRGTTRYMSPELIRHGIVDYAVDTWA 305

Query: 275 LGCVVLEMLTGKPAWSFENANKYMLD--------CYNNELLPEIPKGMSSAGRDFIKRCL 334
            GC VLEMLTG+  W  E+++   +D        CY    +P IP  +S   + F+ RCL
Sbjct: 306 FGCTVLEMLTGQQVWG-EHSDLGSVDWDILIGQSCY----IPYIPDWLSEEAQHFLSRCL 365

Query: 335 IRSPYKRRPIWLLIKHPFV 340
            R P  R  I  L+ HPF+
Sbjct: 366 KRDPASRWGIGALLNHPFL 369

[Close]

BLAST of Spg022631 vs. TAIR 10
Match: AT5G67080.1 (mitogen-activated protein kinase kinase kinase 19 )

HSP 1 Score: 168.7 bits (426), Expect = 8.1e-42
Identity = 91/255 (35.69%), Postives = 142/255 (55.69%), Query Frame = 0


Query: 91  NPNSDLPTEIVVKSCLMEFSSSLQHEKEVLSDLA-PCTNIVHCYGDEITTTKSGEKVYNV 150
           N + + P  + VKS     ++SL +EK VL +L   C  IV C+G++  T ++GE+++N+
Sbjct: 25  NDSGEFPPLMAVKSADSYGAASLANEKSVLDNLGDDCNEIVRCFGED-RTVENGEEMHNL 84

Query: 151 LFEYCSGSSLRDHIINFGLNGLPEYEVRKYTRDIVHGLYHMHCNARYVHGDIKPRNILLL 210
             EY S  SL  ++      G+PE  VR++T  ++ GL H+H N  + H D+K  NILL 
Sbjct: 85  FLEYASRGSLESYLKKLAGEGVPESTVRRHTGSVLRGLRHIHANG-FAHCDLKLGNILLF 144

Query: 211 PSACAADKGSLVAKMASFGLAKKLTPEVVCDQ--EMSGSGPYISPELARDGYLDWPADIW 270
                   G    K+A FGLAK++      +   ++ G+  Y++PE   D       D+W
Sbjct: 145 --------GDGAVKIADFGLAKRIGDLTALNYGVQIRGTPLYMAPESVNDNEYGSEGDVW 204

Query: 271 ALGCVVLEMLTGKPAWSFENANKYM---LDCYNNELLPEIPKGMSSAGRDFIKRCLIRSP 330
           ALGCVV+EM +GK AWS +  + +M   L     + +P IP+ +S  GRDF+ +C ++ P
Sbjct: 205 ALGCVVVEMFSGKTAWSLKEGSNFMSLLLRIGVGDEVPMIPEELSEQGRDFLSKCFVKDP 264

Query: 331 YKRRPIWLLIKHPFV 340
            KR    +L+ HPFV
Sbjct: 265 KKRWTAEMLLNHPFV 269

[Close]

BLAST of Spg022631 vs. TAIR 10
Match: AT3G45790.1 (Protein kinase superfamily protein )

HSP 1 Score: 165.2 bits (417), Expect = 9.0e-41
Identity = 111/304 (36.51%), Postives = 158/304 (51.97%), Query Frame = 0


Query: 35  KKTLPDNNWNDSVKHYNGRFANLTVEESHGSCWIGDRLLRKRGSGLVFLAKNREPTNPNS 94
           K+  P ++ + SV+    +  +  V +S  S WI    L +   G V+LA     T+  +
Sbjct: 66  KRFTPKSSSSLSVEEITKQVFDGVVRKS--SSWIKSEFLGRGSYGSVYLA-----TSKKA 125

Query: 95  DLPTEIVVKSCLMEFSSSLQHEKEVLSDLAPCTNIVHCYGDEIT---TTKSGEKV-YNVL 154
              T + +KS  +  +SSL  E+ +L+ L+    IV CYG EI    T   GE+  YN++
Sbjct: 126 KTKTTMAIKSAEISRASSLMDEERILTRLS-SPFIVRCYGHEIAREETLFGGERTNYNLI 185

Query: 155 FEYCSGSSLRDHIINFGLNGLPEYEVRKYTRDIVHGLYHMHCNARYVHGDIKPRNILLLP 214
            EYCSG SL D ++N  L GL E +V+   RDI++GL ++H  A  +H DIKP NILL P
Sbjct: 186 LEYCSGKSLFD-LVNSNLGGLSEKDVKLLARDILYGLDYIH-RANIIHCDIKPENILLAP 245

Query: 215 SACAADKGSLVAKMASFGLA--KKLTPEVVCDQEMSGSGPYISPELARDGYLDWPADIWA 274
                     VAK+  FGLA  K  +          G+  Y+SPEL R G +D+  D WA
Sbjct: 246 VENRIRPNGYVAKIGDFGLALEKGSSEYEKASGHRRGTTRYMSPELIRHGIVDYAVDTWA 305

Query: 275 LGCVVLEMLTGKPAWSFENANKYMLD--------CYNNELLPEIPKGMSSAGRDFIKRCL 325
            GC VLEMLTG+  W  E+++   +D        CY    +P IP  +S   + F+ RCL
Sbjct: 306 FGCTVLEMLTGQQVWG-EHSDLGSVDWDILIGQSCY----IPYIPDWLSEEAQHFLSRCL 354

The following BLAST results are available for this feature:

BLAST of Spg022631 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023516554.1	3.7e-145	73.16	mitogen-activated protein kinase kinase kinase 17-like [Cucurbita pepo subsp. pe...	[more]
KAG7023508.1	6.9e-144	72.86	Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosper...	[more]
XP_022960664.1	9.0e-144	72.57	mitogen-activated protein kinase kinase kinase 17-like [Cucurbita moschata]	[more]
KAG6589837.1	1.2e-143	72.57	Mitogen-activated protein kinase kinase kinase 20, partial [Cucurbita argyrosper...	[more]
XP_038880300.1	3.0e-131	67.54	mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida]	[more]

BLAST of Spg022631 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SND6	5.5e-43	35.42	Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370...	[more]
Q9CAD5	5.9e-29	34.51	Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3...	[more]
Q54R82	1.7e-28	28.66	Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=...	[more]
F4HRJ4	3.2e-27	31.91	Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702...	[more]
Q61084	3.9e-25	31.94	Mitogen-activated protein kinase kinase kinase 3 OS=Mus musculus OX=10090 GN=Map...	[more]

BLAST of Spg022631 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HBP5	4.4e-144	72.57	mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=...	[more]
A0A5D3D3K9	9.4e-131	68.51	Mitogen-activated protein kinase kinase kinase YODA-like OS=Cucumis melo var. ma...	[more]
A0A1S3B829	9.4e-131	68.51	mitogen-activated protein kinase kinase kinase YODA-like OS=Cucumis melo OX=3656...	[more]
A0A0A0LHV1	2.1e-130	68.22	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G829...	[more]
A0A6J1JAH8	7.5e-104	77.25	mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita maxima OX=36...	[more]

BLAST of Spg022631 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G36950.1	7.1e-46	39.20	mitogen-activated protein kinase kinase kinase 21	[more]
AT3G50310.1	3.9e-44	35.42	mitogen-activated protein kinase kinase kinase 20	[more]
AT3G46140.1	5.6e-43	36.36	Protein kinase superfamily protein	[more]
AT5G67080.1	8.1e-42	35.69	mitogen-activated protein kinase kinase kinase 19	[more]
AT3G45790.1	9.0e-41	36.51	Protein kinase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 67..339 e-value: 1.5E-43 score: 160.7
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 111..339 e-value: 4.3E-35 score: 121.4
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 67..339 score: 33.403603
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 70..341 e-value: 8.6E-47 score: 161.9
None	No IPR available	PANTHER	PTHR48011	CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATED	coord: 54..339
None	No IPR available	PANTHER	PTHR48011:SF55	SUBFAMILY NOT NAMED	coord: 54..339
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 196..208
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 70..339

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg022631.1	Spg022631.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity