Spg022624 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg022624
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionAluminum-activated malate transporter
Locationscaffold2: 14847447 .. 14852228 (-)
RNA-Seq ExpressionSpg022624
SyntenySpg022624
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTGGCTCATCTCTCTCTCTCAATTGCAAATAATTCTCTGTTGCTTGAGCAATGGAGATGGCTGATCTAAAGAGTGAGGGAGTCTTGGCTCGTTGGGGCGAGGGGCTCAAAGCCAAATCTTCAAAGCTAAAGGCTAAGGTGGTCGAGTTGTTTGGTAAGACCAAGAAGCTAGCCAAGGATGATCCGAGGAGAATCGTTCATTCTCTCAAAGTTGGCTTGGCCATCACTTTGGTGTCGTTGTTCTACTACTTCGAGCCGCTCTATGATGGCCTCGGCGCCTCCGCTATGTGGGCTGTCTTAACCGTCGTCGTCGTCTTCGAGTTCTCCGTGGGTAACTATATTCTGATTCTCGTTGCAATGTGGTTGTCACTCTTGTAATTGTTAGATGTGGTGTTTTATTTATTTTGGTTGTTGGGATAATAGGAGCAACACTTGGACGAGGATTGAATAGAGTGTTGGCTACATTTTTGGCTGCGGCTCTGGGATTTGGAGCCCATTTTTTGGCGGATTTGGCTGGAGATGAAGCACAACCTATTATGCTTGCATGCTTTGTTTTCTTTCTTGGTATGTAATTCTACTCCCAACTAGACTTTCATGATTTTGTTTTTTGTTTTTTGAAATTTATGTTTCTTTGTCGTTTCTTTATTATATTTTTGTCTTTTTTAAAAGAAAATGTTGGAATTCTTAGTTAAATTTTGAAAAGAAAAATAAGCTTTTACATTTTACATCTCGTTTGATGACCATTTGGTTTTTGAAAATTGAACTTATAAACATTACTTCCACCTATTGGATTTTTTTTTTTTTTTGTTTTTTTTTGTTTTTTTGTTTTTGTTATCTACCTCTACCCATGTTTTCAAAAACCAAGTTAAGGTTTGAAAACTAAAAAAAGTAGTTTTCGAAAAACTTGTTTTTGTTTTTGAAATTTGGCTAAAAATTCAAATGAGACCGACCTTAACAAAGATGAAAAGCATTATAAGAAAATTAGGGGAAAACAAGTCTAATTTTCAAAAACCAAAATCCAAATGGGTATAAAAAAAACGAGGCTTTAGTTTTCAAATTAATTTTGATTTTGATGTTGCAAACTTTTTAGAATAAATATAATGAAATAAGAAGCTTATAGATGGAAGCAGTATTTACAAGTTTTTTTAATTTTCAAAAATCAAATGGTTATCGTAAATGAGACATTGAAAAGTAATCTTTTGTATTTCTTTTGGGTTAAAGAAAATAAATATGTAATATAAAGTAGTCATCTTTCTTTTAATAATGATTTCTTTAGATTTTCTTTTTACCTTGGCATGATTTCTCTTGACAAAGCTTCATCAATCCTATGAATATCTAACTTTTTAAGGAATCTGCAACTTAATGATATATCTAAAGATATTAGATAAAGAAAGTATTTGGGAAGATTATTAGTGGGACATCTTGAATTTTAAATCTCTATACCTTTTCTTTTTCCATATATGATATTCAAATCAAATAATATTTGTTATTGAATTATTACTATTATTTTTTTTTTTGACAGTTGTTATAGAATTATTATATTTTTTCGAAACATGTTATTGAATTATTGTAGATCCTTGTGATGAATACTAATTGTTGAATAGAAAAATATGGCCTTAAGCTTATCACTATAACTTTAATCATTGTATTTGACATTAGATTTAGCTTTTAAATTATCAAAGATAGCAATAATAGAAATTTTAAAAACTTTCATATAAAATTCAAAATTATATATATATTTTTTTATCCATTGTCAACTGGAAAAAAAAATATATGAAATTAGAAAGAAAAAAAAAACTAAAAAGGATTAAATTATGATTTTTTTTCAATGACATTATGAATTTGATCCCTATGACTTGAACTTAATTTCAATATGATCTAGTTTTTTAGAGTATCAATTTAGTTCTTATGAGACTTAGGGTATGTTTGGGTCTGATTTTAAGATGGAAAAATGATTATGGAAAACTTTTAACTTTTGTAGGTGTTTGATAAAACTAAATTTTTATTGATTTAAATACAATCATTTTTCAGTTACTTTGGTTTGGAGAATCTCATAAGAAGATGCTTTTGAAATTAGCTTTCCCGTGTAAAATTACTATGATATCCTTGCCTAGTTAAGCTTTTCCTATTTGGCAATTTGGAAGCAAGTTTTTTTTTTTCTTTGTTTCTTTTTCATGAAATGAGGTGTCATATGTTTTTTTTTTTTTCTCATTATTTTCAAGTCATAATGACAAACAAAAATTGTATTATTCAATAAGTCAGTTTTATGTTTGATATTTATTATTTTAGGAAACAAGTTATTTCAAAAATTTATTATTATTTTTGTTTTTATGTTTTGATCTATTAATTAATTCAATATTTTTTGTTATATAAAAATTATTTTTCCTAATCTTTTATCAAATAATTTAGTGTAATTTGAATATAATTTTTATAATTGAAATCATATAAAGTATAAAATAATTGTCTTCTAACAATTTTAAATATTAAAAATTAATATAAAAATAATTGAAAATAAAATATCTATTTTAGTAATTATAGCTTAATCACAATAATTTAGTATATTAATTTACCAAACACTCTAACTTGCTTCTTGAGGTCTAAAGTTGAAATTTACGAAACGCTAATTTGCTTATTATTATAGCTAATTTTTCTCAAAGCACATATGACAACCATGGTTATTTTAACCGCCACAACAATCCCAAACTAAGTCTTCGTTTTAAACATTAAGGTCTTGTTTGATAATTTTTTGTTTTTCATATTTAAAAATTCTTCTTGCTTTTTTCCACAATTTCTTTACAATGTTTTCATCTTTTTCTTCAAAGAAACATATGAATTTTTAGCCAAATTTCAAAAACAAAAACATGTTGTTAAAAACTGTAGTGTAAGAATCAAATTGAAAAAAAAAACTATGTATGGATGTATATGTTGATAGATAGTATTTGATTATGAATATAATGCATAATGCAGCTGCAACAACAACATTCGTGCGATTCTTCCCTCGAATAAAGGCGAGATACGACTATGGCTTCCTCATCTTCATCTTGACCTTCTGTTTAGTATCAGTTTCTGGTTACAGAGACGACGAGATTCTTCAAGTGGCCTACACCAGAGCTCTCACCATTCTCATCGGCACCTTCATCGCCGTTCTCATCTGTGTTTTCATCTGCCCCGTTTGGGCTGGCCACGATCTCCATTCCCTTCTCTCCAACAACATTCAACTCCTCGCCAACTTCCTCCAAGGTCTTCCCACACTCTCTCTCTTATCATTGGTTCATTTTAGCAAAACAACGATACTTGGCTGCATATCGGTAGATGGTCCGTAAACAAACTCAGTTTTGATCTTTATTTTTTTTGCTTTGCAGATTTTGGGGATCGATTTTCGAGTAGTGAATCGAAAGGAGATGAGCTTCTACAAGGATGCAAGAGTGTTTTGACGTCAAGGCAAACCGAAGAGTCGTTGGTTAACTTTGCAAGGTGGGAACCACGCCATGGGAGCTTCAAGTTTCGTCATCCATGGAAGCAATATCGGAAGATTGGTAGCTTGACCAGGCAATGTGCTTATCGCCTCGAGTCTCTCAACAGCTACCTTCTCACTGAAGCTCAAGTAAGACACTCTCTTCCTTCAAACAATTAGGTTAAACTATTAGATAACATTGCCTCTCCAAGGTTGAATTCACTATTCTTAAATCGGATTCCAATTTTTTTCGGACCGAATTCGTTTGGGTTTCATGGACTCTGATACCATATTAGATAACATAAGATTCCATCTCAAAACCTATTCACGAGTAATTCATATTTTATAAATTGTGAGATCTCCTGCACTTTTCTTTTTAACGTGGGATCCTCAACATAAACTACTCCATTTGATCTCAAATTGCATTTTTTCTATTTACATTATTTTAGATGAATAATGTAAATGGATCCGAGAAAATAGGTGGGTAAATAGAAAAAAATAGCTTATTATATAGATTGAAAATGTGTCACATATTTAATAACGTCGAGAACTAATTTATAGAGACAATTTGTATAGATTTAACCAAATTATATAAGCAAATTTTATGTGAAATTACAAAATACTATAAAACTAATCTCAAACCATGTGGAACAAATAGATAATTTAGCCCTTGAAAAAACACATGCCAAAGGAAGTAAAATTAATGAATTTATTGAACTTCGTCCATTTGTTTCATGCAGACCCCATTACAGATCCGAGACCAACTCAAGGAGTCCTGCTCAAGAATGAGCATTGAATCAGGCAAAGCTCTGAAGGATCTAGCATCTGCAATAAGAACGATGACCAGACCGACGTTTCCGAATCCTCACATCGAGAAGTCGAAGGCAGCAGCGAAGAACCTGAAGGCAGCGCTGAAGATCGGGCCGTGCGACGATGGTATTGATCTTCTCGAGATAGTACCGACGGCGACTGTGGCTTCTTTGCTCATTGATTCCATTTCTTGCATAGAGAAGATTGCAGAGTCAGTTGGTGAATTGGCCTCACTTGCACATTTCAAGTGTGTGGAAGCTGGTAAATCAGCTGGTTTGGAATTGGAACAAGAGCAGCAAAAGTTGCCAACTCCAGCCATTGTTAATGACCATTGTCATGTTGTTACTATAGATTAATGATAATGTCACACTTTACCAATCTTAACTGTGATCCATATGTATATGGTATAATATATGAGCTTTTAGGAACTATAATAACAAATGACTACCTTTTTGAAGCCACTTTAATATATCGCTTTCTTTTTAAATTATTTATTTTTAGTCCTATGGTATTACCT

mRNA sequence

ATGGAGATGGCTGATCTAAAGAGTGAGGGAGTCTTGGCTCGTTGGGGCGAGGGGCTCAAAGCCAAATCTTCAAAGCTAAAGGCTAAGGTGGTCGAGTTGTTTGGTAAGACCAAGAAGCTAGCCAAGGATGATCCGAGGAGAATCGTTCATTCTCTCAAAGTTGGCTTGGCCATCACTTTGGTGTCGTTGTTCTACTACTTCGAGCCGCTCTATGATGGCCTCGGCGCCTCCGCTATGTGGGCTGTCTTAACCGTCGTCGTCGTCTTCGAGTTCTCCGTGGGAGCAACACTTGGACGAGGATTGAATAGAGTGTTGGCTACATTTTTGGCTGCGGCTCTGGGATTTGGAGCCCATTTTTTGGCGGATTTGGCTGGAGATGAAGCACAACCTATTATGCTTGCATGCTTTGTTTTCTTTCTTGCTGCAACAACAACATTCGTGCGATTCTTCCCTCGAATAAAGGCGAGATACGACTATGGCTTCCTCATCTTCATCTTGACCTTCTGTTTAGTATCAGTTTCTGGTTACAGAGACGACGAGATTCTTCAAGTGGCCTACACCAGAGCTCTCACCATTCTCATCGGCACCTTCATCGCCGTTCTCATCTGTGTTTTCATCTGCCCCGTTTGGGCTGGCCACGATCTCCATTCCCTTCTCTCCAACAACATTCAACTCCTCGCCAACTTCCTCCAAGATTTTGGGGATCGATTTTCGAGTAGTGAATCGAAAGGAGATGAGCTTCTACAAGGATGCAAGAGTGTTTTGACGTCAAGGCAAACCGAAGAGTCGTTGGTTAACTTTGCAAGGTGGGAACCACGCCATGGGAGCTTCAAGTTTCGTCATCCATGGAAGCAATATCGGAAGATTGGTAGCTTGACCAGGCAATGTGCTTATCGCCTCGAGTCTCTCAACAGCTACCTTCTCACTGAAGCTCAAACCCCATTACAGATCCGAGACCAACTCAAGGAGTCCTGCTCAAGAATGAGCATTGAATCAGGCAAAGCTCTGAAGGATCTAGCATCTGCAATAAGAACGATGACCAGACCGACGTTTCCGAATCCTCACATCGAGAAGTCGAAGGCAGCAGCGAAGAACCTGAAGGCAGCGCTGAAGATCGGGCCGTGCGACGATGGTATTGATCTTCTCGAGATAGTACCGACGGCGACTGTGGCTTCTTTGCTCATTGATTCCATTTCTTGCATAGAGAAGATTGCAGAGTCAGTTGGTGAATTGGCCTCACTTGCACATTTCAAGTGTGTGGAAGCTGGTAAATCAGCTGGTTTGGAATTGGAACAAGAGCAGCAAAAGTTGCCAACTCCAGCCATTGTTAATGACCATTGTCATGTTGTTACTATAGATTAA

Coding sequence (CDS)

ATGGAGATGGCTGATCTAAAGAGTGAGGGAGTCTTGGCTCGTTGGGGCGAGGGGCTCAAAGCCAAATCTTCAAAGCTAAAGGCTAAGGTGGTCGAGTTGTTTGGTAAGACCAAGAAGCTAGCCAAGGATGATCCGAGGAGAATCGTTCATTCTCTCAAAGTTGGCTTGGCCATCACTTTGGTGTCGTTGTTCTACTACTTCGAGCCGCTCTATGATGGCCTCGGCGCCTCCGCTATGTGGGCTGTCTTAACCGTCGTCGTCGTCTTCGAGTTCTCCGTGGGAGCAACACTTGGACGAGGATTGAATAGAGTGTTGGCTACATTTTTGGCTGCGGCTCTGGGATTTGGAGCCCATTTTTTGGCGGATTTGGCTGGAGATGAAGCACAACCTATTATGCTTGCATGCTTTGTTTTCTTTCTTGCTGCAACAACAACATTCGTGCGATTCTTCCCTCGAATAAAGGCGAGATACGACTATGGCTTCCTCATCTTCATCTTGACCTTCTGTTTAGTATCAGTTTCTGGTTACAGAGACGACGAGATTCTTCAAGTGGCCTACACCAGAGCTCTCACCATTCTCATCGGCACCTTCATCGCCGTTCTCATCTGTGTTTTCATCTGCCCCGTTTGGGCTGGCCACGATCTCCATTCCCTTCTCTCCAACAACATTCAACTCCTCGCCAACTTCCTCCAAGATTTTGGGGATCGATTTTCGAGTAGTGAATCGAAAGGAGATGAGCTTCTACAAGGATGCAAGAGTGTTTTGACGTCAAGGCAAACCGAAGAGTCGTTGGTTAACTTTGCAAGGTGGGAACCACGCCATGGGAGCTTCAAGTTTCGTCATCCATGGAAGCAATATCGGAAGATTGGTAGCTTGACCAGGCAATGTGCTTATCGCCTCGAGTCTCTCAACAGCTACCTTCTCACTGAAGCTCAAACCCCATTACAGATCCGAGACCAACTCAAGGAGTCCTGCTCAAGAATGAGCATTGAATCAGGCAAAGCTCTGAAGGATCTAGCATCTGCAATAAGAACGATGACCAGACCGACGTTTCCGAATCCTCACATCGAGAAGTCGAAGGCAGCAGCGAAGAACCTGAAGGCAGCGCTGAAGATCGGGCCGTGCGACGATGGTATTGATCTTCTCGAGATAGTACCGACGGCGACTGTGGCTTCTTTGCTCATTGATTCCATTTCTTGCATAGAGAAGATTGCAGAGTCAGTTGGTGAATTGGCCTCACTTGCACATTTCAAGTGTGTGGAAGCTGGTAAATCAGCTGGTTTGGAATTGGAACAAGAGCAGCAAAAGTTGCCAACTCCAGCCATTGTTAATGACCATTGTCATGTTGTTACTATAGATTAA

Protein sequence

MEMADLKSEGVLARWGEGLKAKSSKLKAKVVELFGKTKKLAKDDPRRIVHSLKVGLAITLVSLFYYFEPLYDGLGASAMWAVLTVVVVFEFSVGATLGRGLNRVLATFLAAALGFGAHFLADLAGDEAQPIMLACFVFFLAATTTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDDEILQVAYTRALTILIGTFIAVLICVFICPVWAGHDLHSLLSNNIQLLANFLQDFGDRFSSSESKGDELLQGCKSVLTSRQTEESLVNFARWEPRHGSFKFRHPWKQYRKIGSLTRQCAYRLESLNSYLLTEAQTPLQIRDQLKESCSRMSIESGKALKDLASAIRTMTRPTFPNPHIEKSKAAAKNLKAALKIGPCDDGIDLLEIVPTATVASLLIDSISCIEKIAESVGELASLAHFKCVEAGKSAGLELEQEQQKLPTPAIVNDHCHVVTID
Homology
BLAST of Spg022624 vs. NCBI nr
Match: XP_038880035.1 (aluminum-activated malate transporter 8-like [Benincasa hispida])

HSP 1 Score: 754.6 bits (1947), Expect = 4.9e-214
Identity = 391/454 (86.12%), Postives = 422/454 (92.95%), Query Frame = 0

Query: 1   MEMADLKSEGVLARWGEGLKAKSSKLKAKVVELFGKTKKLAKDDPRRIVHSLKVGLAITL 60
           MEMA+ KSEGVLARWGEGL+AK SKLKAKV+ELF KTKKLAKDDPRRIVHSLKVGLA+TL
Sbjct: 1   MEMANDKSEGVLARWGEGLEAKFSKLKAKVIELFRKTKKLAKDDPRRIVHSLKVGLALTL 60

Query: 61  VSLFYYFEPLYDGLGASAMWAVLTVVVVFEFSVGATLGRGLNRVLATFLAAALGFGAHFL 120
           VSLFYYFEPLYDGLGASAMWA+LTVVVVFEFSVGATLGRGLNRVLATF AAALGFGAHFL
Sbjct: 61  VSLFYYFEPLYDGLGASAMWAILTVVVVFEFSVGATLGRGLNRVLATFFAAALGFGAHFL 120

Query: 121 ADLAGDEAQPIMLACFVFFLAATTTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDDE 180
           ADLAGD+AQPIML+  VFFLA  TTFVRFFPR+KARYDYGFLIFILTFCLVSVSGYR+DE
Sbjct: 121 ADLAGDKAQPIMLSLSVFFLATITTFVRFFPRLKARYDYGFLIFILTFCLVSVSGYREDE 180

Query: 181 ILQVAYTRALTILIGTFIAVLICVFICPVWAGHDLHSLLSNNIQLLANFLQDFGDRFSSS 240
           IL VAY RALTILIGTFIA+LIC+ ICPVWAG DLHSL+SNNI+LLANFL+ FG ++ S+
Sbjct: 181 ILNVAYRRALTILIGTFIAILICICICPVWAGDDLHSLVSNNIELLANFLEGFGGQY-SN 240

Query: 241 ESKGDELLQGCKSVLTSRQTEESLVNFARWEPRHGSFKFRHPWKQYRKIGSLTRQCAYRL 300
           E K +E+++ CKSVLTSRQTEESLVNFARWEP HG+FKFRHPWKQYRKIGSLTRQCAYRL
Sbjct: 241 EWKENEVVEECKSVLTSRQTEESLVNFARWEPGHGTFKFRHPWKQYRKIGSLTRQCAYRL 300

Query: 301 ESLNSYLLTEAQTPLQIRDQLKESCSRMSIESGKALKDLASAIRTMTRPTFPNPHIEKSK 360
           ESLN+YL+TE QTPL IRDQLKESCS+MSIES KALK LASAIRTMTRPTFPNPHIE+SK
Sbjct: 301 ESLNTYLVTECQTPLHIRDQLKESCSKMSIESSKALKVLASAIRTMTRPTFPNPHIERSK 360

Query: 361 AAAKNLKAALKIGPCDDGIDLLEIVPTATVASLLIDSISCIEKIAESVGELASLAHFKCV 420
           A AK+LKAALKI PCD G+DLLEIVP ATVASLLIDSISCIEKIAESVGELASLA+FKCV
Sbjct: 361 AVAKDLKAALKIRPCDSGMDLLEIVPMATVASLLIDSISCIEKIAESVGELASLANFKCV 420

Query: 421 EAGKSAGLELEQEQ-QKLPTPAIVNDHCHVVTID 454
           E GKSA ++LEQ Q QKLPTPAIVN HCHVVTID
Sbjct: 421 EVGKSASMKLEQHQEQKLPTPAIVNGHCHVVTID 453

BLAST of Spg022624 vs. NCBI nr
Match: XP_023515628.1 (aluminum-activated malate transporter 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 750.4 bits (1936), Expect = 9.3e-213
Identity = 388/451 (86.03%), Postives = 417/451 (92.46%), Query Frame = 0

Query: 3   MADLKSEGVLARWGEGLKAKSSKLKAKVVELFGKTKKLAKDDPRRIVHSLKVGLAITLVS 62
           MAD K+EGVLARWG+GLKAKSS L AKVVEL  KTKKLAKDDPRRIVHS KVGLAITLVS
Sbjct: 1   MADEKNEGVLARWGKGLKAKSSSLMAKVVELLRKTKKLAKDDPRRIVHSFKVGLAITLVS 60

Query: 63  LFYYFEPLYDGLGASAMWAVLTVVVVFEFSVGATLGRGLNRVLATFLAAALGFGAHFLAD 122
           LFYYFEPLYDGLGASAMWAVLTVVVVFEFSVGATLGRGLNRVLAT LAA+LGFGAHFLAD
Sbjct: 61  LFYYFEPLYDGLGASAMWAVLTVVVVFEFSVGATLGRGLNRVLATSLAASLGFGAHFLAD 120

Query: 123 LAGDEAQPIMLACFVFFLAATTTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDDEIL 182
           LAGD+AQP++L+  VFF AA TTF+RFFPRIKARYDYGFLIFILTFCLVSVSGYRDDEIL
Sbjct: 121 LAGDKAQPVILSLSVFFFAAITTFIRFFPRIKARYDYGFLIFILTFCLVSVSGYRDDEIL 180

Query: 183 QVAYTRALTILIGTFIAVLICVFICPVWAGHDLHSLLSNNIQLLANFLQDFGDRFSSSES 242
           QVAY RALTIL+GTFIA+LIC+FICPVWAG DLHSL+SNNIQLLANFLQ FG ++ S+ES
Sbjct: 181 QVAYRRALTILLGTFIAILICLFICPVWAGDDLHSLVSNNIQLLANFLQGFGVQY-SNES 240

Query: 243 KGDELLQGCKSVLTSRQTEESLVNFARWEPRHGSFKFRHPWKQYRKIGSLTRQCAYRLES 302
           K DELL+GCKSVLTSRQTEESLVNFARWEPRHG FKFRHPWKQYRKIGSLTRQCAYRLES
Sbjct: 241 KEDELLEGCKSVLTSRQTEESLVNFARWEPRHGPFKFRHPWKQYRKIGSLTRQCAYRLES 300

Query: 303 LNSYLLTEAQTPLQIRDQLKESCSRMSIESGKALKDLASAIRTMTRPTFPNPHIEKSKAA 362
           LNSYL+TE QTPL IRD+L +SCS+MSIES  AL+D+AS +RTMT+PTFPNPHIEKSKAA
Sbjct: 301 LNSYLITETQTPLHIRDRLMKSCSKMSIESSNALEDIASDLRTMTQPTFPNPHIEKSKAA 360

Query: 363 AKNLKAALKIGPCDDGIDLLEIVPTATVASLLIDSISCIEKIAESVGELASLAHFKCVEA 422
            K+LKAALKIGP D  IDLLEIVP ATVASLLID+ISCIEKIAESV EL SLA+FKCVEA
Sbjct: 361 MKDLKAALKIGPGDGDIDLLEIVPIATVASLLIDTISCIEKIAESVCELGSLANFKCVEA 420

Query: 423 GKSAGLELEQEQQKLPTPAIVNDHCHVVTID 454
           GKS+GLELEQE QKLPT AIVNDHCHVV +D
Sbjct: 421 GKSSGLELEQEHQKLPTSAIVNDHCHVVMVD 450

BLAST of Spg022624 vs. NCBI nr
Match: XP_022960624.1 (aluminum-activated malate transporter 2-like [Cucurbita moschata])

HSP 1 Score: 750.4 bits (1936), Expect = 9.3e-213
Identity = 387/451 (85.81%), Postives = 417/451 (92.46%), Query Frame = 0

Query: 3   MADLKSEGVLARWGEGLKAKSSKLKAKVVELFGKTKKLAKDDPRRIVHSLKVGLAITLVS 62
           MAD K+EGVLARWGEGLKAKSS LKAKVVEL  KTKKLAKDDPRRIVHS KVGLAITLVS
Sbjct: 1   MADEKNEGVLARWGEGLKAKSSSLKAKVVELLRKTKKLAKDDPRRIVHSFKVGLAITLVS 60

Query: 63  LFYYFEPLYDGLGASAMWAVLTVVVVFEFSVGATLGRGLNRVLATFLAAALGFGAHFLAD 122
           LFYYFEPLYDGLGASAMWAVLTVVVVFEFSVGATLGRGLNRVLAT LAA+LGFGAHFLAD
Sbjct: 61  LFYYFEPLYDGLGASAMWAVLTVVVVFEFSVGATLGRGLNRVLATSLAASLGFGAHFLAD 120

Query: 123 LAGDEAQPIMLACFVFFLAATTTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDDEIL 182
           LAGD+AQP++L+  VFF A  TTF+RFFPRIKARYDYGFLIFILTFCLVSVSGYRDDEIL
Sbjct: 121 LAGDKAQPVILSLSVFFFATITTFIRFFPRIKARYDYGFLIFILTFCLVSVSGYRDDEIL 180

Query: 183 QVAYTRALTILIGTFIAVLICVFICPVWAGHDLHSLLSNNIQLLANFLQDFGDRFSSSES 242
           QVAY RALTIL+GTFIA+LIC+FICPVWAG DLHSL+SNNIQLLANFLQ FG ++ S+ES
Sbjct: 181 QVAYRRALTILLGTFIAILICLFICPVWAGDDLHSLVSNNIQLLANFLQGFGVQY-SNES 240

Query: 243 KGDELLQGCKSVLTSRQTEESLVNFARWEPRHGSFKFRHPWKQYRKIGSLTRQCAYRLES 302
           K DELL+GCKSVLTSRQTEESLVNFARWEPRHG FKFRHPWKQY+KIGSLTRQCAYRLES
Sbjct: 241 KEDELLEGCKSVLTSRQTEESLVNFARWEPRHGPFKFRHPWKQYQKIGSLTRQCAYRLES 300

Query: 303 LNSYLLTEAQTPLQIRDQLKESCSRMSIESGKALKDLASAIRTMTRPTFPNPHIEKSKAA 362
           LN+YL+TE QTPL IRD+L +SCS+MSIES  AL+D+AS +RTMT+PTFPNPHIEKSKAA
Sbjct: 301 LNNYLVTETQTPLHIRDRLMKSCSKMSIESSNALEDIASDLRTMTQPTFPNPHIEKSKAA 360

Query: 363 AKNLKAALKIGPCDDGIDLLEIVPTATVASLLIDSISCIEKIAESVGELASLAHFKCVEA 422
            K+LKAALKIGP D  IDLLEIVP ATVASLLID+ISCIEKIAESVGEL SLA+FKCVEA
Sbjct: 361 MKDLKAALKIGPGDGDIDLLEIVPIATVASLLIDTISCIEKIAESVGELGSLANFKCVEA 420

Query: 423 GKSAGLELEQEQQKLPTPAIVNDHCHVVTID 454
           GKS+ LELEQE QKLPT AIVNDHCHVV +D
Sbjct: 421 GKSSDLELEQEHQKLPTSAIVNDHCHVVIVD 450

BLAST of Spg022624 vs. NCBI nr
Match: XP_022141905.1 (aluminum-activated malate transporter 2-like [Momordica charantia])

HSP 1 Score: 750.0 bits (1935), Expect = 1.2e-212
Identity = 393/453 (86.75%), Postives = 415/453 (91.61%), Query Frame = 0

Query: 1   MEMADLKSEGV-LARWGEGLKAKSSKLKAKVVELFGKTKKLAKDDPRRIVHSLKVGLAIT 60
           MEMAD KSEGV  ARW EGL+AKSS+L  KV EL  KTKKLAKDDPRRI HSLKV LAIT
Sbjct: 1   MEMADHKSEGVSSARWAEGLRAKSSRLTVKVGELARKTKKLAKDDPRRIAHSLKVALAIT 60

Query: 61  LVSLFYYFEPLYDGLGASAMWAVLTVVVVFEFSVGATLGRGLNRVLATFLAAALGFGAHF 120
           LVSLFYYFEPLYDG GASAMWA+LTVVVVFEFSVGATLGRGLNRVLATF AAALGFGAHF
Sbjct: 61  LVSLFYYFEPLYDGFGASAMWAILTVVVVFEFSVGATLGRGLNRVLATFSAAALGFGAHF 120

Query: 121 LADLAGDEAQPIMLACFVFFLAATTTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDD 180
           LADLA D+ +PI+LA FVF LAATTTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDD
Sbjct: 121 LADLAADKGEPIILAFFVFLLAATTTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDD 180

Query: 181 EILQVAYTRALTILIGTFIAVLICVFICPVWAGHDLHSLLSNNIQLLANFLQDFGDRFSS 240
           EILQVAYTRALTILIGTFIA+LIC+FICPVWAG DLHSL+SNN++LL NFLQ FG R+ S
Sbjct: 181 EILQVAYTRALTILIGTFIAILICIFICPVWAGEDLHSLVSNNVELLGNFLQGFGARY-S 240

Query: 241 SESKGDELLQGCKSVLTSRQTEESLVNFARWEPRHGSFKFRHPWKQYRKIGSLTRQCAYR 300
            E KGD+ ++GCKSVLTSRQTEESLVNFARWEP HGSFKFRHPWKQYRKIGSLTRQCAYR
Sbjct: 241 DEWKGDKQVEGCKSVLTSRQTEESLVNFARWEPGHGSFKFRHPWKQYRKIGSLTRQCAYR 300

Query: 301 LESLNSYLLTEAQTPLQIRDQLKESCSRMSIESGKALKDLASAIRTMTRPTFPNPHIEKS 360
           LESLN YL TE Q PL IRDQLK+SCS+MSIESGKALKDLASAIRTMTRPTFPNPH+EKS
Sbjct: 301 LESLNGYLTTETQIPLHIRDQLKDSCSKMSIESGKALKDLASAIRTMTRPTFPNPHVEKS 360

Query: 361 KAAAKNLKAALKIGPCDDGIDLLEIVPTATVASLLIDSISCIEKIAESVGELASLAHF-K 420
           KAAAKNLK ALKIGPC+DGIDLLEIVP ATVASLLIDSISCIEKIAESVGELAS+A F K
Sbjct: 361 KAAAKNLKVALKIGPCNDGIDLLEIVPAATVASLLIDSISCIEKIAESVGELASMAQFKK 420

Query: 421 CVEAGKSAGLELEQEQQK-LPTPAIVNDHCHVV 451
           CVEAGK AGLELEQ+QQ+ LPTPAIVN HCHVV
Sbjct: 421 CVEAGKLAGLELEQDQQQNLPTPAIVNGHCHVV 452

BLAST of Spg022624 vs. NCBI nr
Match: XP_022988110.1 (aluminum-activated malate transporter 2-like [Cucurbita maxima])

HSP 1 Score: 748.8 bits (1932), Expect = 2.7e-212
Identity = 387/451 (85.81%), Postives = 417/451 (92.46%), Query Frame = 0

Query: 3   MADLKSEGVLARWGEGLKAKSSKLKAKVVELFGKTKKLAKDDPRRIVHSLKVGLAITLVS 62
           M+D K+EGVLARWGEGLKAKSS LKAKVVEL  KTKKLAKDDPRRIVHS KVGLAITLVS
Sbjct: 1   MSDEKNEGVLARWGEGLKAKSSSLKAKVVELLRKTKKLAKDDPRRIVHSFKVGLAITLVS 60

Query: 63  LFYYFEPLYDGLGASAMWAVLTVVVVFEFSVGATLGRGLNRVLATFLAAALGFGAHFLAD 122
           LFYYFEPLYDGLGASAMWAVLTVVVVFEFSVGATLGRGLNRVLAT LAA+LGFGAHFLAD
Sbjct: 61  LFYYFEPLYDGLGASAMWAVLTVVVVFEFSVGATLGRGLNRVLATSLAASLGFGAHFLAD 120

Query: 123 LAGDEAQPIMLACFVFFLAATTTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDDEIL 182
           LAGD+AQP++L+  VF  AA TTF+RFFPRIKARYDYGFLIFILTFCLVSVSGYRDDEIL
Sbjct: 121 LAGDKAQPVILSLSVFIFAAITTFIRFFPRIKARYDYGFLIFILTFCLVSVSGYRDDEIL 180

Query: 183 QVAYTRALTILIGTFIAVLICVFICPVWAGHDLHSLLSNNIQLLANFLQDFGDRFSSSES 242
           QVAY RALTIL+GTFIA+LIC+FICPVWAG DLHSL+SNNIQLLANFLQ FG ++ S+ES
Sbjct: 181 QVAYRRALTILLGTFIAILICLFICPVWAGDDLHSLVSNNIQLLANFLQGFGVQY-SNES 240

Query: 243 KGDELLQGCKSVLTSRQTEESLVNFARWEPRHGSFKFRHPWKQYRKIGSLTRQCAYRLES 302
           K DELL+GCKSVLTSRQTEESLVNFARWEPRHG+FKFRHPWKQYRKIGSLTRQ AYRLES
Sbjct: 241 KEDELLEGCKSVLTSRQTEESLVNFARWEPRHGAFKFRHPWKQYRKIGSLTRQSAYRLES 300

Query: 303 LNSYLLTEAQTPLQIRDQLKESCSRMSIESGKALKDLASAIRTMTRPTFPNPHIEKSKAA 362
           LNSYL+TE QTPL IRD+L +SCS+MSIES  AL+D+AS +RTMT+PTFPNPHIEKSKAA
Sbjct: 301 LNSYLVTETQTPLHIRDRLMKSCSKMSIESSNALEDIASDLRTMTQPTFPNPHIEKSKAA 360

Query: 363 AKNLKAALKIGPCDDGIDLLEIVPTATVASLLIDSISCIEKIAESVGELASLAHFKCVEA 422
            K+LKAALKIGP D  IDLLEIVP ATVASLLID+ISCIEKIAESVGEL SLA+FKCVEA
Sbjct: 361 MKDLKAALKIGPGDGDIDLLEIVPIATVASLLIDTISCIEKIAESVGELGSLANFKCVEA 420

Query: 423 GKSAGLELEQEQQKLPTPAIVNDHCHVVTID 454
           GKS+GLELEQE QKLP  AIVNDHCHVV +D
Sbjct: 421 GKSSGLELEQEHQKLPNSAIVNDHCHVVIVD 450

BLAST of Spg022624 vs. ExPASy Swiss-Prot
Match: Q9SJE8 (Aluminum-activated malate transporter 2 OS=Arabidopsis thaliana OX=3702 GN=ALMT2 PE=2 SV=2)

HSP 1 Score: 420.2 bits (1079), Expect = 2.9e-116
Identity = 222/432 (51.39%), Postives = 305/432 (70.60%), Query Frame = 0

Query: 29  KVVELFGKTKKLAKDDPRRIVHSLKVGLAITLVSLFYYFEPLYDGLGASAMWAVLTVVVV 88
           KV E+  + +++ K+DPRR+VH+ KVGLA+ LVS FYY++PLYD  G +AMWAV+TVVVV
Sbjct: 3   KVREIVREGRRVGKEDPRRVVHAFKVGLALALVSSFYYYQPLYDNFGVNAMWAVMTVVVV 62

Query: 89  FEFSVGATLGRGLNRVLATFLAAALGFGAHFLADLAGDEAQPIMLACFVFFLAATTTFVR 148
           FEFSVGATLG+GLNR +AT +A  LG GAH LA L+G   +PI+LA FVF LAA +TFVR
Sbjct: 63  FEFSVGATLGKGLNRAVATLVAGGLGIGAHHLASLSGPTVEPILLAIFVFVLAALSTFVR 122

Query: 149 FFPRIKARYDYGFLIFILTFCLVSVSGYRDDEILQVAYTRALTILIGTFIAVLICVFICP 208
           FFPR+KARYDYG LIFILTF L+SVSG+R+DEIL +A+ R  T+++G    VLI +F+CP
Sbjct: 123 FFPRVKARYDYGVLIFILTFALISVSGFREDEILDLAHKRLSTVIMGGVSCVLISIFVCP 182

Query: 209 VWAGHDLHSLLSNNIQLLANFLQDFGDRFSSSESKGD--------ELLQGCKSVLTSRQT 268
           VWAG DLHSLL++N   L++FLQ+FGD +  +   GD          L+  KSVL S+  
Sbjct: 183 VWAGQDLHSLLASNFDTLSHFLQEFGDEYFEATEDGDIKEVEKRRRNLERYKSVLNSKSN 242

Query: 269 EESLVNFARWEPRHGSFKFRHPWKQYRKIGSLTRQCAYRLESLNSYLLTEAQTPLQIRDQ 328
           EE+L NFA+WEPRHG F+FRHPW+QY  +G+L RQ AYR+++LNS + ++ Q P+ I+ +
Sbjct: 243 EEALANFAKWEPRHGQFRFRHPWRQYLAVGALLRQSAYRIDALNSNINSDMQIPMDIKKK 302

Query: 329 LKESCSRMSIESGKALKDLASAIRTMTRPTFPNPHIEKSKAAAKNLKAALKIGPCDDGID 388
           ++E   RMS ESGK++K+++ +++ MT  +  + H+  S++A K L   LK G  +D ++
Sbjct: 303 IEEPLRRMSSESGKSMKEVSISLKNMTISSSFDIHVVNSQSACKTLSTLLKSGILND-VE 362

Query: 389 LLEIVPTATVASLLIDSISCIEKIAESVGELASLAHFKCVEAGKSAGLELEQEQQKLPTP 448
            L+++   T  SLLID ++  EKI+ESV ELAS A FK     K +        Q +P  
Sbjct: 363 PLQMISLMTTVSLLIDIVNLTEKISESVHELASAAKFK--NKKKPSKSNSGSIGQAMPNK 422

Query: 449 AIVNDHCHVVTI 453
           +  +D  HVVTI
Sbjct: 423 S-HDDDDHVVTI 430

BLAST of Spg022624 vs. ExPASy Swiss-Prot
Match: Q9SRM9 (Aluminum-activated malate transporter 8 OS=Arabidopsis thaliana OX=3702 GN=ALMT8 PE=3 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 8.1e-111
Identity = 219/418 (52.39%), Postives = 281/418 (67.22%), Query Frame = 0

Query: 5   DLKSEGVLARWGEGLKAKSSKLKAKVVELFGKTKKLAKDDPRRIVHSLKVGLAITLVSLF 64
           DL ++   A + + L+   SKLK  V +     +K AKDDPRRI+HS+KVG+A+TLVSL 
Sbjct: 2   DLNAQEKKAGFFQRLQDFPSKLKDDVTKRVKNVQKFAKDDPRRIIHSMKVGVALTLVSLL 61

Query: 65  YYFEPLYDGLGASAMWAVLTVVVVFEFSVGATLGRGLNRVLATFLAAALGFGAHFLADLA 124
           YY  PLY   G + MWA+LTVVVVFEF+VG TL +GLNR  AT +A ALG GA  LA   
Sbjct: 62  YYVRPLYISFGVTGMWAILTVVVVFEFTVGGTLSKGLNRGFATLIAGALGVGAVHLARFF 121

Query: 125 GDEAQPIMLACFVFFLAATTTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDDEILQV 184
           G + +PI+L   VF L A  TF RFFPRIK RYDYG LIFILTF  V++SGYR DEIL +
Sbjct: 122 GHQGEPIVLGILVFSLGAAATFSRFFPRIKQRYDYGALIFILTFSFVAISGYRTDEILIM 181

Query: 185 AYTRALTILIGTFIAVLICVFICPVWAGHDLHSLLSNNIQLLANFLQDFGDRFSSSESKG 244
           AY R  TILIG  I +L+ +FICPVWAG DLH +++NNI  LA +L+ F   +   E   
Sbjct: 182 AYQRLSTILIGGTICILVSIFICPVWAGEDLHKMIANNINKLAKYLEGFEGEYFQPEKIS 241

Query: 245 DE---LLQGCKSVLTSRQTEESLVNFARWEPRHGSFKFRHPWKQYRKIGSLTRQCAYRLE 304
            E    ++  KS+LTS+ TE+SL N ARWEP HG F+ RHPWK+Y KI  L RQCA  LE
Sbjct: 242 KETSSCVREYKSILTSKSTEDSLANLARWEPGHGRFRLRHPWKKYLKIAGLVRQCAVHLE 301

Query: 305 SLNSYLLTEAQTPLQIRDQLKESCSRMSIESGKALKDLASAIRTMTRPT-FPNPHIEKSK 364
            LN Y+L+  + P +   +++E  + MS E G+ALK +A +I+TM   +   N HI+ SK
Sbjct: 302 ILNGYVLSNDKAPQEFESKIQEPITTMSREVGEALKAIAKSIKTMRNDSACVNAHIDNSK 361

Query: 365 AAAKNLKAALKIGPCDDGIDLLEIVPTATVASLLIDSISCIEKIAESVGELASLAHFK 419
            A KNLK ALK    +   DLLEI+P  T+AS+LI+ ++C+EKI E+V E + LAHFK
Sbjct: 362 KAIKNLKIALKSSYPETYKDLLEIIPGVTMASILIEVVNCVEKIYEAVEEFSGLAHFK 419

BLAST of Spg022624 vs. ExPASy Swiss-Prot
Match: Q9XIN1 (Aluminum-activated malate transporter 7 OS=Arabidopsis thaliana OX=3702 GN=ALMT7 PE=3 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 2.0e-109
Identity = 209/424 (49.29%), Postives = 285/424 (67.22%), Query Frame = 0

Query: 29  KVVELFGKTKKLAKDDPRRIVHSLKVGLAITLVSLFYYFEPLYDGLGASAMWAVLTVVVV 88
           KV E+  + +++AK+DPRR+VHS KVGL + LVS FYY++PLYD  G +AMWAV+TVVVV
Sbjct: 3   KVREIVREGRRVAKEDPRRVVHSFKVGLVLALVSSFYYYQPLYDSFGVNAMWAVMTVVVV 62

Query: 89  FEFSVGATLGRGLNRVLATFLAAALGFGAHFLADLAGDEAQPIMLACFVFFLAATTTFVR 148
           FEFSVGATLG+GLNRV AT  A  LG GAH LA ++G   +PI+LA FVF  AA +TFVR
Sbjct: 63  FEFSVGATLGKGLNRVAATLFAGGLGIGAHHLASMSGPTGEPILLAVFVFVQAALSTFVR 122

Query: 149 FFPRIKARYDYGFLIFILTFCLVSVSGYRDDEILQVAYTRALTILIGTFIAVLICVFICP 208
           FFPR+KARYDY  LIFILTF L+SVSG+R+++++++ + R  T++IG    V+I +F+CP
Sbjct: 123 FFPRVKARYDYSLLIFILTFALISVSGFREEQVVKLTHKRISTVIIGGLSCVIISIFVCP 182

Query: 209 VWAGHDLHSLLSNNIQLLANF--------------------------LQDFGDRF----- 268
           VWAG DLHSL+++N + L+ F                          L DFGD++     
Sbjct: 183 VWAGQDLHSLIASNFEKLSFFLLGNSFHYVSSDLNSITLLRKIKSWRLADFGDKYCEVVE 242

Query: 269 ---SSSESKGDELLQGCKSVLTSRQTEESLVNFARWEPRHGSFKFRHPWKQYRKIGSLTR 328
              +    K  +     KSVL S+  EESL NFA+WEP HG F+FRHPWKQY  +G L R
Sbjct: 243 NDGAKEVDKRKKDFDNYKSVLNSKSNEESLANFAKWEPGHGQFRFRHPWKQYLAVGELIR 302

Query: 329 QCAYRLESLNSYLLTEAQTPLQIRDQLKESCSRMSIESGKALKDLASAIRTMTRPTFPNP 388
           QCAYR+ +LNSYL  + Q  + I+ +L E   RMS+ESGKA+K+++ +++ MT+P+  + 
Sbjct: 303 QCAYRIHALNSYLNADNQVSVDIKKKLGEPLRRMSLESGKAMKEMSISLKKMTKPSSSDL 362

Query: 389 HIEKSKAAAKNLKAALKIGPCDDGIDLLEIVPTATVASLLIDSISCIEKIAESVGELASL 419
           H++ +K+A K+L   L  G   + ++ LE+V   T  SLLID I+  EKI ES+ ELA+ 
Sbjct: 363 HVQNAKSACKSLTNLLNSGILKE-VEPLELVSLLTAISLLIDIINLTEKILESLHELATA 422

BLAST of Spg022624 vs. ExPASy Swiss-Prot
Match: Q9SJE9 (Aluminum-activated malate transporter 1 OS=Arabidopsis thaliana OX=3702 GN=ALMT1 PE=1 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 1.9e-107
Identity = 202/401 (50.37%), Postives = 284/401 (70.82%), Query Frame = 0

Query: 29  KVVELFGKTKKLAKDDPRRIVHSLKVGLAITLVSLFYYFE---PLYDGLGASAMWAVLTV 88
           KV E+  +  ++  +DPRRI+H+ KVGLA+ LVS FYY++   P  D  G +AMWAV+TV
Sbjct: 3   KVREIVREGIRVGNEDPRRIIHAFKVGLALVLVSSFYYYQPFGPFTDYFGINAMWAVMTV 62

Query: 89  VVVFEFSVGATLGRGLNRVLATFLAAALGFGAHFLADLAGDEAQPIMLACFVFFLAATTT 148
           VVVFEFSVGATLG+GLNR +AT +A  LG GAH LA L+G   +PI+L   VF  AA +T
Sbjct: 63  VVVFEFSVGATLGKGLNRGVATLVAGGLGIGAHQLARLSGATVEPILLVMLVFVQAALST 122

Query: 149 FVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDDEILQVAYTRALTILIGTFIAVLICVF 208
           FVRFFP +K ++DYG LIFILTF L+S+SG+RD+EI+ +A +R  T++IG    +LI +F
Sbjct: 123 FVRFFPWVKTKFDYGILIFILTFALISLSGFRDEEIMDLAESRLSTVVIGGVSCILISIF 182

Query: 209 ICPVWAGHDLHSLLSNNIQLLANFLQDFGDRFSSSESKGD--------ELLQGCKSVLTS 268
           +CPVWAG DLHSLL++N   L++FLQDFGD +  +  KGD        + L+  KSVL S
Sbjct: 183 VCPVWAGQDLHSLLASNFDTLSHFLQDFGDEYFEAREKGDYKVVEKRKKNLERYKSVLDS 242

Query: 269 RQTEESLVNFARWEPRHGSFKFRHPWKQYRKIGSLTRQCAYRLESLNSYLLTEAQTPLQI 328
           +  EE+L N+A WEP HG F+FRHPWKQY  +G+L RQCAYR+++LNSY+ ++ Q P+ I
Sbjct: 243 KSDEEALANYAEWEPPHGQFRFRHPWKQYVAVGALLRQCAYRIDALNSYINSDFQIPVDI 302

Query: 329 RDQLKESCSRMSIESGKALKDLASAIRTMTRPTFPNPHIEKSKAAAKNLKAALKIGPCDD 388
           + +L+    RMS ESG ++K+++ +++ M + +  + H+  S+AA K+L   LK G  +D
Sbjct: 303 KKKLETPLRRMSSESGNSMKEMSISLKQMIKSSSSDIHVSNSQAACKSLSTLLKSGILND 362

Query: 389 GIDLLEIVPTATVASLLIDSISCIEKIAESVGELASLAHFK 419
            ++ L+++   T  S+LID ++  EKI+ESV ELAS A FK
Sbjct: 363 -VEPLQMISLMTTVSMLIDIVNLTEKISESVHELASAARFK 402

BLAST of Spg022624 vs. ExPASy Swiss-Prot
Match: Q76LB1 (Aluminum-activated malate transporter 1 OS=Triticum aestivum OX=4565 GN=ALMT1 PE=1 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 8.7e-97
Identity = 203/454 (44.71%), Postives = 289/454 (63.66%), Query Frame = 0

Query: 4   ADLKSEGVLARWGEGLKAKSSKLKAKVVELFGKTKKLAKDDPRRIVHSLKVGLAITLVSL 63
           +D +  G +A  G  L +  + L  +      K    A++DPRR+ HSLKVGLA+ LVS+
Sbjct: 9   SDGEMVGTIASCGLLLHSLLAGLGRRAAGFARKVGGAAREDPRRVAHSLKVGLALALVSV 68

Query: 64  FYYFEPLYDGLGASAMWAVLTVVVVFEFSVGATLGRGLNRVLATFLAAALGFGAHFLADL 123
            Y+  PL++GLG SA+WAVLTVVVV E++VGATL +GLNR LAT +A  +  GAH LA+L
Sbjct: 69  VYFVTPLFNGLGVSAIWAVLTVVVVMEYTVGATLSKGLNRALATLVAGCIAVGAHQLAEL 128

Query: 124 A---GDEAQPIMLACFVFFLAATTTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDDE 183
           A   GD+ +PI+L   VFF+A+  TF+RF P IKA+YDYG  IFILTF LV+VS YR +E
Sbjct: 129 AERCGDQGEPIVLTVLVFFVASAATFLRFIPEIKAKYDYGVTIFILTFGLVAVSSYRVEE 188

Query: 184 ILQVAYTRALTILIGTFIAVLICVFICPVWAGHDLHSLLSNNIQLLANFLQD-----FGD 243
           ++Q+A+ R  TI +G FI +   VF+ PVWAG D+H L S N+  LA F++      FG+
Sbjct: 189 LIQLAHQRFYTIAVGVFICLCTTVFLFPVWAGEDVHKLASGNLDKLAQFIEGMEFNCFGE 248

Query: 244 RFSSSESKGDELLQGCKSVLTSRQTEESLVNFARWEPRHGSFKFRHPWKQYRKIGSLTRQ 303
              ++   G +  Q  KSVL S+ TE+SL  FA+WEPRHG F+FRHPW QY+K+G+L RQ
Sbjct: 249 NSVANNFGGKDSPQMHKSVLNSKATEDSLCTFAKWEPRHGQFRFRHPWSQYQKLGTLCRQ 308

Query: 304 CAYRLESLNSYLLTEAQTPL------QIRDQLKESCSRMSIESGKALKDLASAIRTMTRP 363
           CA  +E+L SY++T ++T        ++  +++++C  MS+ S K L+DLA A RTMT P
Sbjct: 309 CASSMEALASYVITTSKTQCPAAANPELSCKVRKTCGEMSLHSSKVLRDLAMATRTMTVP 368

Query: 364 TFPNPHIEKSKAAAKNLKAALKIGPCDDGIDLLEIVPTATVASLLIDSISCIEKIAESVG 423
           +  N  +  +  AA++L++ L      +   LL+++  A  A+LL D +  +++IAE V 
Sbjct: 369 SPVNITMATAVKAAESLRSELA-----ENTALLQVMHVAVTATLLADLVDRVKEIAECVD 428

Query: 424 ELASLAHFKCVEAGKSAGLELEQEQQKLPTPAIV 444
            LA LAHFK  E  K+  +         P P +V
Sbjct: 429 VLARLAHFKNPEDTKNVVVSTVSRGIDEPLPDVV 457

BLAST of Spg022624 vs. ExPASy TrEMBL
Match: A0A6J1HBI7 (aluminum-activated malate transporter 2-like OS=Cucurbita moschata OX=3662 GN=LOC111461355 PE=3 SV=1)

HSP 1 Score: 750.4 bits (1936), Expect = 4.5e-213
Identity = 387/451 (85.81%), Postives = 417/451 (92.46%), Query Frame = 0

Query: 3   MADLKSEGVLARWGEGLKAKSSKLKAKVVELFGKTKKLAKDDPRRIVHSLKVGLAITLVS 62
           MAD K+EGVLARWGEGLKAKSS LKAKVVEL  KTKKLAKDDPRRIVHS KVGLAITLVS
Sbjct: 1   MADEKNEGVLARWGEGLKAKSSSLKAKVVELLRKTKKLAKDDPRRIVHSFKVGLAITLVS 60

Query: 63  LFYYFEPLYDGLGASAMWAVLTVVVVFEFSVGATLGRGLNRVLATFLAAALGFGAHFLAD 122
           LFYYFEPLYDGLGASAMWAVLTVVVVFEFSVGATLGRGLNRVLAT LAA+LGFGAHFLAD
Sbjct: 61  LFYYFEPLYDGLGASAMWAVLTVVVVFEFSVGATLGRGLNRVLATSLAASLGFGAHFLAD 120

Query: 123 LAGDEAQPIMLACFVFFLAATTTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDDEIL 182
           LAGD+AQP++L+  VFF A  TTF+RFFPRIKARYDYGFLIFILTFCLVSVSGYRDDEIL
Sbjct: 121 LAGDKAQPVILSLSVFFFATITTFIRFFPRIKARYDYGFLIFILTFCLVSVSGYRDDEIL 180

Query: 183 QVAYTRALTILIGTFIAVLICVFICPVWAGHDLHSLLSNNIQLLANFLQDFGDRFSSSES 242
           QVAY RALTIL+GTFIA+LIC+FICPVWAG DLHSL+SNNIQLLANFLQ FG ++ S+ES
Sbjct: 181 QVAYRRALTILLGTFIAILICLFICPVWAGDDLHSLVSNNIQLLANFLQGFGVQY-SNES 240

Query: 243 KGDELLQGCKSVLTSRQTEESLVNFARWEPRHGSFKFRHPWKQYRKIGSLTRQCAYRLES 302
           K DELL+GCKSVLTSRQTEESLVNFARWEPRHG FKFRHPWKQY+KIGSLTRQCAYRLES
Sbjct: 241 KEDELLEGCKSVLTSRQTEESLVNFARWEPRHGPFKFRHPWKQYQKIGSLTRQCAYRLES 300

Query: 303 LNSYLLTEAQTPLQIRDQLKESCSRMSIESGKALKDLASAIRTMTRPTFPNPHIEKSKAA 362
           LN+YL+TE QTPL IRD+L +SCS+MSIES  AL+D+AS +RTMT+PTFPNPHIEKSKAA
Sbjct: 301 LNNYLVTETQTPLHIRDRLMKSCSKMSIESSNALEDIASDLRTMTQPTFPNPHIEKSKAA 360

Query: 363 AKNLKAALKIGPCDDGIDLLEIVPTATVASLLIDSISCIEKIAESVGELASLAHFKCVEA 422
            K+LKAALKIGP D  IDLLEIVP ATVASLLID+ISCIEKIAESVGEL SLA+FKCVEA
Sbjct: 361 MKDLKAALKIGPGDGDIDLLEIVPIATVASLLIDTISCIEKIAESVGELGSLANFKCVEA 420

Query: 423 GKSAGLELEQEQQKLPTPAIVNDHCHVVTID 454
           GKS+ LELEQE QKLPT AIVNDHCHVV +D
Sbjct: 421 GKSSDLELEQEHQKLPTSAIVNDHCHVVIVD 450

BLAST of Spg022624 vs. ExPASy TrEMBL
Match: A0A6J1CJE7 (aluminum-activated malate transporter 2-like OS=Momordica charantia OX=3673 GN=LOC111012160 PE=3 SV=1)

HSP 1 Score: 750.0 bits (1935), Expect = 5.9e-213
Identity = 393/453 (86.75%), Postives = 415/453 (91.61%), Query Frame = 0

Query: 1   MEMADLKSEGV-LARWGEGLKAKSSKLKAKVVELFGKTKKLAKDDPRRIVHSLKVGLAIT 60
           MEMAD KSEGV  ARW EGL+AKSS+L  KV EL  KTKKLAKDDPRRI HSLKV LAIT
Sbjct: 1   MEMADHKSEGVSSARWAEGLRAKSSRLTVKVGELARKTKKLAKDDPRRIAHSLKVALAIT 60

Query: 61  LVSLFYYFEPLYDGLGASAMWAVLTVVVVFEFSVGATLGRGLNRVLATFLAAALGFGAHF 120
           LVSLFYYFEPLYDG GASAMWA+LTVVVVFEFSVGATLGRGLNRVLATF AAALGFGAHF
Sbjct: 61  LVSLFYYFEPLYDGFGASAMWAILTVVVVFEFSVGATLGRGLNRVLATFSAAALGFGAHF 120

Query: 121 LADLAGDEAQPIMLACFVFFLAATTTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDD 180
           LADLA D+ +PI+LA FVF LAATTTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDD
Sbjct: 121 LADLAADKGEPIILAFFVFLLAATTTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDD 180

Query: 181 EILQVAYTRALTILIGTFIAVLICVFICPVWAGHDLHSLLSNNIQLLANFLQDFGDRFSS 240
           EILQVAYTRALTILIGTFIA+LIC+FICPVWAG DLHSL+SNN++LL NFLQ FG R+ S
Sbjct: 181 EILQVAYTRALTILIGTFIAILICIFICPVWAGEDLHSLVSNNVELLGNFLQGFGARY-S 240

Query: 241 SESKGDELLQGCKSVLTSRQTEESLVNFARWEPRHGSFKFRHPWKQYRKIGSLTRQCAYR 300
            E KGD+ ++GCKSVLTSRQTEESLVNFARWEP HGSFKFRHPWKQYRKIGSLTRQCAYR
Sbjct: 241 DEWKGDKQVEGCKSVLTSRQTEESLVNFARWEPGHGSFKFRHPWKQYRKIGSLTRQCAYR 300

Query: 301 LESLNSYLLTEAQTPLQIRDQLKESCSRMSIESGKALKDLASAIRTMTRPTFPNPHIEKS 360
           LESLN YL TE Q PL IRDQLK+SCS+MSIESGKALKDLASAIRTMTRPTFPNPH+EKS
Sbjct: 301 LESLNGYLTTETQIPLHIRDQLKDSCSKMSIESGKALKDLASAIRTMTRPTFPNPHVEKS 360

Query: 361 KAAAKNLKAALKIGPCDDGIDLLEIVPTATVASLLIDSISCIEKIAESVGELASLAHF-K 420
           KAAAKNLK ALKIGPC+DGIDLLEIVP ATVASLLIDSISCIEKIAESVGELAS+A F K
Sbjct: 361 KAAAKNLKVALKIGPCNDGIDLLEIVPAATVASLLIDSISCIEKIAESVGELASMAQFKK 420

Query: 421 CVEAGKSAGLELEQEQQK-LPTPAIVNDHCHVV 451
           CVEAGK AGLELEQ+QQ+ LPTPAIVN HCHVV
Sbjct: 421 CVEAGKLAGLELEQDQQQNLPTPAIVNGHCHVV 452

BLAST of Spg022624 vs. ExPASy TrEMBL
Match: A0A6J1JIP2 (aluminum-activated malate transporter 2-like OS=Cucurbita maxima OX=3661 GN=LOC111485453 PE=3 SV=1)

HSP 1 Score: 748.8 bits (1932), Expect = 1.3e-212
Identity = 387/451 (85.81%), Postives = 417/451 (92.46%), Query Frame = 0

Query: 3   MADLKSEGVLARWGEGLKAKSSKLKAKVVELFGKTKKLAKDDPRRIVHSLKVGLAITLVS 62
           M+D K+EGVLARWGEGLKAKSS LKAKVVEL  KTKKLAKDDPRRIVHS KVGLAITLVS
Sbjct: 1   MSDEKNEGVLARWGEGLKAKSSSLKAKVVELLRKTKKLAKDDPRRIVHSFKVGLAITLVS 60

Query: 63  LFYYFEPLYDGLGASAMWAVLTVVVVFEFSVGATLGRGLNRVLATFLAAALGFGAHFLAD 122
           LFYYFEPLYDGLGASAMWAVLTVVVVFEFSVGATLGRGLNRVLAT LAA+LGFGAHFLAD
Sbjct: 61  LFYYFEPLYDGLGASAMWAVLTVVVVFEFSVGATLGRGLNRVLATSLAASLGFGAHFLAD 120

Query: 123 LAGDEAQPIMLACFVFFLAATTTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDDEIL 182
           LAGD+AQP++L+  VF  AA TTF+RFFPRIKARYDYGFLIFILTFCLVSVSGYRDDEIL
Sbjct: 121 LAGDKAQPVILSLSVFIFAAITTFIRFFPRIKARYDYGFLIFILTFCLVSVSGYRDDEIL 180

Query: 183 QVAYTRALTILIGTFIAVLICVFICPVWAGHDLHSLLSNNIQLLANFLQDFGDRFSSSES 242
           QVAY RALTIL+GTFIA+LIC+FICPVWAG DLHSL+SNNIQLLANFLQ FG ++ S+ES
Sbjct: 181 QVAYRRALTILLGTFIAILICLFICPVWAGDDLHSLVSNNIQLLANFLQGFGVQY-SNES 240

Query: 243 KGDELLQGCKSVLTSRQTEESLVNFARWEPRHGSFKFRHPWKQYRKIGSLTRQCAYRLES 302
           K DELL+GCKSVLTSRQTEESLVNFARWEPRHG+FKFRHPWKQYRKIGSLTRQ AYRLES
Sbjct: 241 KEDELLEGCKSVLTSRQTEESLVNFARWEPRHGAFKFRHPWKQYRKIGSLTRQSAYRLES 300

Query: 303 LNSYLLTEAQTPLQIRDQLKESCSRMSIESGKALKDLASAIRTMTRPTFPNPHIEKSKAA 362
           LNSYL+TE QTPL IRD+L +SCS+MSIES  AL+D+AS +RTMT+PTFPNPHIEKSKAA
Sbjct: 301 LNSYLVTETQTPLHIRDRLMKSCSKMSIESSNALEDIASDLRTMTQPTFPNPHIEKSKAA 360

Query: 363 AKNLKAALKIGPCDDGIDLLEIVPTATVASLLIDSISCIEKIAESVGELASLAHFKCVEA 422
            K+LKAALKIGP D  IDLLEIVP ATVASLLID+ISCIEKIAESVGEL SLA+FKCVEA
Sbjct: 361 MKDLKAALKIGPGDGDIDLLEIVPIATVASLLIDTISCIEKIAESVGELGSLANFKCVEA 420

Query: 423 GKSAGLELEQEQQKLPTPAIVNDHCHVVTID 454
           GKS+GLELEQE QKLP  AIVNDHCHVV +D
Sbjct: 421 GKSSGLELEQEHQKLPNSAIVNDHCHVVIVD 450

BLAST of Spg022624 vs. ExPASy TrEMBL
Match: A0A5D3DX63 (Aluminum-activated malate transporter 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold289G00170 PE=3 SV=1)

HSP 1 Score: 727.2 bits (1876), Expect = 4.1e-206
Identity = 375/454 (82.60%), Postives = 411/454 (90.53%), Query Frame = 0

Query: 1   MEMADLKSEGVLARWGEGLKAKSSKLKAKVVELFGKTKKLAKDDPRRIVHSLKVGLAITL 60
           MEM++ KS G LARW +GLKAK SKLKAKV+ELF KTKKLAKDDPRRIVHSLKVGLAITL
Sbjct: 1   MEMSNEKSGGALARWSDGLKAKISKLKAKVIELFKKTKKLAKDDPRRIVHSLKVGLAITL 60

Query: 61  VSLFYYFEPLYDGLGASAMWAVLTVVVVFEFSVGATLGRGLNRVLATFLAAALGFGAHFL 120
           VSLFYYFEPLYDGLGASAMWA+LTVVVVFEFSVGAT GRGLNRVLATFLAAALGFG HFL
Sbjct: 61  VSLFYYFEPLYDGLGASAMWAILTVVVVFEFSVGATFGRGLNRVLATFLAAALGFGVHFL 120

Query: 121 ADLAGDEAQPIMLACFVFFLAATTTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDDE 180
           ADLAGD+AQPIML+  VFFLAA TTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYR+DE
Sbjct: 121 ADLAGDKAQPIMLSLSVFFLAAITTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYREDE 180

Query: 181 ILQVAYTRALTILIGTFIAVLICVFICPVWAGHDLHSLLSNNIQLLANFLQDFGDRFSSS 240
           IL+VAY RALTILIGTFIA+LIC+ ICPVWAG DLHSL+SNNI+ LANF Q FG  +S+ 
Sbjct: 181 ILKVAYRRALTILIGTFIAILICILICPVWAGDDLHSLVSNNIEQLANFFQGFGVEYSNE 240

Query: 241 ESKGDELLQGCKSVLTSRQTEESLVNFARWEPRHGSFKFRHPWKQYRKIGSLTRQCAYRL 300
             + + +++G KSVL+SRQTEESLVNFARWEP HG+FKFRHPWKQY +IGSLTRQCAYRL
Sbjct: 241 WKEDERIVEGFKSVLSSRQTEESLVNFARWEPPHGTFKFRHPWKQYSRIGSLTRQCAYRL 300

Query: 301 ESLNSYLLTEAQTPLQIRDQLKESCSRMSIESGKALKDLASAIRTMTRPTFPNPHIEKSK 360
           ESLN+YLL E+QTPL IRDQLKESC++MS ESGKALKDLAS+IRTMT P  PNPHIEKSK
Sbjct: 301 ESLNTYLLAESQTPLHIRDQLKESCTKMSTESGKALKDLASSIRTMTLPRLPNPHIEKSK 360

Query: 361 AAAKNLKAALKIGPCDDGIDLLEIVPTATVASLLIDSISCIEKIAESVGELASLAHFKCV 420
           AAAK+LKAALKI PC+  IDLLEIVP ATVASLLIDSISCIEKIAESVGELASLA+FKC+
Sbjct: 361 AAAKDLKAALKIRPCNSSIDLLEIVPMATVASLLIDSISCIEKIAESVGELASLANFKCL 420

Query: 421 EAGKSAGLELEQE-QQKLPTPAIVNDHCHVVTID 454
           E GKSA L+ +QE QQKL TPAIV+ HCHVVT+D
Sbjct: 421 EVGKSASLKFQQEQQQKLATPAIVSGHCHVVTVD 454

BLAST of Spg022624 vs. ExPASy TrEMBL
Match: A0A1S3BNY9 (aluminum-activated malate transporter 2-like OS=Cucumis melo OX=3656 GN=LOC103491946 PE=3 SV=1)

HSP 1 Score: 727.2 bits (1876), Expect = 4.1e-206
Identity = 375/454 (82.60%), Postives = 411/454 (90.53%), Query Frame = 0

Query: 1   MEMADLKSEGVLARWGEGLKAKSSKLKAKVVELFGKTKKLAKDDPRRIVHSLKVGLAITL 60
           MEM++ KS G LARW +GLKAK SKLKAKV+ELF KTKKLAKDDPRRIVHSLKVGLAITL
Sbjct: 1   MEMSNEKSGGALARWSDGLKAKISKLKAKVIELFKKTKKLAKDDPRRIVHSLKVGLAITL 60

Query: 61  VSLFYYFEPLYDGLGASAMWAVLTVVVVFEFSVGATLGRGLNRVLATFLAAALGFGAHFL 120
           VSLFYYFEPLYDGLGASAMWA+LTVVVVFEFSVGAT GRGLNRVLATFLAAALGFG HFL
Sbjct: 61  VSLFYYFEPLYDGLGASAMWAILTVVVVFEFSVGATFGRGLNRVLATFLAAALGFGVHFL 120

Query: 121 ADLAGDEAQPIMLACFVFFLAATTTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDDE 180
           ADLAGD+AQPIML+  VFFLAA TTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYR+DE
Sbjct: 121 ADLAGDKAQPIMLSLSVFFLAAITTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYREDE 180

Query: 181 ILQVAYTRALTILIGTFIAVLICVFICPVWAGHDLHSLLSNNIQLLANFLQDFGDRFSSS 240
           IL+VAY RALTILIGTFIA+LIC+ ICPVWAG DLHSL+SNNI+ LANF Q FG  +S+ 
Sbjct: 181 ILKVAYRRALTILIGTFIAILICILICPVWAGDDLHSLVSNNIEQLANFFQGFGVEYSNE 240

Query: 241 ESKGDELLQGCKSVLTSRQTEESLVNFARWEPRHGSFKFRHPWKQYRKIGSLTRQCAYRL 300
             + + +++G KSVL+SRQTEESLVNFARWEP HG+FKFRHPWKQY +IGSLTRQCAYRL
Sbjct: 241 WKEDERIVEGFKSVLSSRQTEESLVNFARWEPPHGTFKFRHPWKQYSRIGSLTRQCAYRL 300

Query: 301 ESLNSYLLTEAQTPLQIRDQLKESCSRMSIESGKALKDLASAIRTMTRPTFPNPHIEKSK 360
           ESLN+YLL E+QTPL IRDQLKESC++MS ESGKALKDLAS+IRTMT P  PNPHIEKSK
Sbjct: 301 ESLNTYLLAESQTPLHIRDQLKESCTKMSTESGKALKDLASSIRTMTLPRLPNPHIEKSK 360

Query: 361 AAAKNLKAALKIGPCDDGIDLLEIVPTATVASLLIDSISCIEKIAESVGELASLAHFKCV 420
           AAAK+LKAALKI PC+  IDLLEIVP ATVASLLIDSISCIEKIAESVGELASLA+FKC+
Sbjct: 361 AAAKDLKAALKIRPCNSSIDLLEIVPMATVASLLIDSISCIEKIAESVGELASLANFKCL 420

Query: 421 EAGKSAGLELEQE-QQKLPTPAIVNDHCHVVTID 454
           E GKSA L+ +QE QQKL TPAIV+ HCHVVT+D
Sbjct: 421 EVGKSASLKFQQEQQQKLATPAIVSGHCHVVTVD 454

BLAST of Spg022624 vs. TAIR 10
Match: AT1G08440.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 420.2 bits (1079), Expect = 2.1e-117
Identity = 222/432 (51.39%), Postives = 305/432 (70.60%), Query Frame = 0

Query: 29  KVVELFGKTKKLAKDDPRRIVHSLKVGLAITLVSLFYYFEPLYDGLGASAMWAVLTVVVV 88
           KV E+  + +++ K+DPRR+VH+ KVGLA+ LVS FYY++PLYD  G +AMWAV+TVVVV
Sbjct: 3   KVREIVREGRRVGKEDPRRVVHAFKVGLALALVSSFYYYQPLYDNFGVNAMWAVMTVVVV 62

Query: 89  FEFSVGATLGRGLNRVLATFLAAALGFGAHFLADLAGDEAQPIMLACFVFFLAATTTFVR 148
           FEFSVGATLG+GLNR +AT +A  LG GAH LA L+G   +PI+LA FVF LAA +TFVR
Sbjct: 63  FEFSVGATLGKGLNRAVATLVAGGLGIGAHHLASLSGPTVEPILLAIFVFVLAALSTFVR 122

Query: 149 FFPRIKARYDYGFLIFILTFCLVSVSGYRDDEILQVAYTRALTILIGTFIAVLICVFICP 208
           FFPR+KARYDYG LIFILTF L+SVSG+R+DEIL +A+ R  T+++G    VLI +F+CP
Sbjct: 123 FFPRVKARYDYGVLIFILTFALISVSGFREDEILDLAHKRLSTVIMGGVSCVLISIFVCP 182

Query: 209 VWAGHDLHSLLSNNIQLLANFLQDFGDRFSSSESKGD--------ELLQGCKSVLTSRQT 268
           VWAG DLHSLL++N   L++FLQ+FGD +  +   GD          L+  KSVL S+  
Sbjct: 183 VWAGQDLHSLLASNFDTLSHFLQEFGDEYFEATEDGDIKEVEKRRRNLERYKSVLNSKSN 242

Query: 269 EESLVNFARWEPRHGSFKFRHPWKQYRKIGSLTRQCAYRLESLNSYLLTEAQTPLQIRDQ 328
           EE+L NFA+WEPRHG F+FRHPW+QY  +G+L RQ AYR+++LNS + ++ Q P+ I+ +
Sbjct: 243 EEALANFAKWEPRHGQFRFRHPWRQYLAVGALLRQSAYRIDALNSNINSDMQIPMDIKKK 302

Query: 329 LKESCSRMSIESGKALKDLASAIRTMTRPTFPNPHIEKSKAAAKNLKAALKIGPCDDGID 388
           ++E   RMS ESGK++K+++ +++ MT  +  + H+  S++A K L   LK G  +D ++
Sbjct: 303 IEEPLRRMSSESGKSMKEVSISLKNMTISSSFDIHVVNSQSACKTLSTLLKSGILND-VE 362

Query: 389 LLEIVPTATVASLLIDSISCIEKIAESVGELASLAHFKCVEAGKSAGLELEQEQQKLPTP 448
            L+++   T  SLLID ++  EKI+ESV ELAS A FK     K +        Q +P  
Sbjct: 363 PLQMISLMTTVSLLIDIVNLTEKISESVHELASAAKFK--NKKKPSKSNSGSIGQAMPNK 422

Query: 449 AIVNDHCHVVTI 453
           +  +D  HVVTI
Sbjct: 423 S-HDDDDHVVTI 430

BLAST of Spg022624 vs. TAIR 10
Match: AT3G11680.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 402.1 bits (1032), Expect = 5.8e-112
Identity = 219/418 (52.39%), Postives = 281/418 (67.22%), Query Frame = 0

Query: 5   DLKSEGVLARWGEGLKAKSSKLKAKVVELFGKTKKLAKDDPRRIVHSLKVGLAITLVSLF 64
           DL ++   A + + L+   SKLK  V +     +K AKDDPRRI+HS+KVG+A+TLVSL 
Sbjct: 2   DLNAQEKKAGFFQRLQDFPSKLKDDVTKRVKNVQKFAKDDPRRIIHSMKVGVALTLVSLL 61

Query: 65  YYFEPLYDGLGASAMWAVLTVVVVFEFSVGATLGRGLNRVLATFLAAALGFGAHFLADLA 124
           YY  PLY   G + MWA+LTVVVVFEF+VG TL +GLNR  AT +A ALG GA  LA   
Sbjct: 62  YYVRPLYISFGVTGMWAILTVVVVFEFTVGGTLSKGLNRGFATLIAGALGVGAVHLARFF 121

Query: 125 GDEAQPIMLACFVFFLAATTTFVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDDEILQV 184
           G + +PI+L   VF L A  TF RFFPRIK RYDYG LIFILTF  V++SGYR DEIL +
Sbjct: 122 GHQGEPIVLGILVFSLGAAATFSRFFPRIKQRYDYGALIFILTFSFVAISGYRTDEILIM 181

Query: 185 AYTRALTILIGTFIAVLICVFICPVWAGHDLHSLLSNNIQLLANFLQDFGDRFSSSESKG 244
           AY R  TILIG  I +L+ +FICPVWAG DLH +++NNI  LA +L+ F   +   E   
Sbjct: 182 AYQRLSTILIGGTICILVSIFICPVWAGEDLHKMIANNINKLAKYLEGFEGEYFQPEKIS 241

Query: 245 DE---LLQGCKSVLTSRQTEESLVNFARWEPRHGSFKFRHPWKQYRKIGSLTRQCAYRLE 304
            E    ++  KS+LTS+ TE+SL N ARWEP HG F+ RHPWK+Y KI  L RQCA  LE
Sbjct: 242 KETSSCVREYKSILTSKSTEDSLANLARWEPGHGRFRLRHPWKKYLKIAGLVRQCAVHLE 301

Query: 305 SLNSYLLTEAQTPLQIRDQLKESCSRMSIESGKALKDLASAIRTMTRPT-FPNPHIEKSK 364
            LN Y+L+  + P +   +++E  + MS E G+ALK +A +I+TM   +   N HI+ SK
Sbjct: 302 ILNGYVLSNDKAPQEFESKIQEPITTMSREVGEALKAIAKSIKTMRNDSACVNAHIDNSK 361

Query: 365 AAAKNLKAALKIGPCDDGIDLLEIVPTATVASLLIDSISCIEKIAESVGELASLAHFK 419
            A KNLK ALK    +   DLLEI+P  T+AS+LI+ ++C+EKI E+V E + LAHFK
Sbjct: 362 KAIKNLKIALKSSYPETYKDLLEIIPGVTMASILIEVVNCVEKIYEAVEEFSGLAHFK 419

BLAST of Spg022624 vs. TAIR 10
Match: AT2G27240.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 397.5 bits (1020), Expect = 1.4e-110
Identity = 209/424 (49.29%), Postives = 285/424 (67.22%), Query Frame = 0

Query: 29  KVVELFGKTKKLAKDDPRRIVHSLKVGLAITLVSLFYYFEPLYDGLGASAMWAVLTVVVV 88
           KV E+  + +++AK+DPRR+VHS KVGL + LVS FYY++PLYD  G +AMWAV+TVVVV
Sbjct: 3   KVREIVREGRRVAKEDPRRVVHSFKVGLVLALVSSFYYYQPLYDSFGVNAMWAVMTVVVV 62

Query: 89  FEFSVGATLGRGLNRVLATFLAAALGFGAHFLADLAGDEAQPIMLACFVFFLAATTTFVR 148
           FEFSVGATLG+GLNRV AT  A  LG GAH LA ++G   +PI+LA FVF  AA +TFVR
Sbjct: 63  FEFSVGATLGKGLNRVAATLFAGGLGIGAHHLASMSGPTGEPILLAVFVFVQAALSTFVR 122

Query: 149 FFPRIKARYDYGFLIFILTFCLVSVSGYRDDEILQVAYTRALTILIGTFIAVLICVFICP 208
           FFPR+KARYDY  LIFILTF L+SVSG+R+++++++ + R  T++IG    V+I +F+CP
Sbjct: 123 FFPRVKARYDYSLLIFILTFALISVSGFREEQVVKLTHKRISTVIIGGLSCVIISIFVCP 182

Query: 209 VWAGHDLHSLLSNNIQLLANF--------------------------LQDFGDRF----- 268
           VWAG DLHSL+++N + L+ F                          L DFGD++     
Sbjct: 183 VWAGQDLHSLIASNFEKLSFFLLGNSFHYVSSDLNSITLLRKIKSWRLADFGDKYCEVVE 242

Query: 269 ---SSSESKGDELLQGCKSVLTSRQTEESLVNFARWEPRHGSFKFRHPWKQYRKIGSLTR 328
              +    K  +     KSVL S+  EESL NFA+WEP HG F+FRHPWKQY  +G L R
Sbjct: 243 NDGAKEVDKRKKDFDNYKSVLNSKSNEESLANFAKWEPGHGQFRFRHPWKQYLAVGELIR 302

Query: 329 QCAYRLESLNSYLLTEAQTPLQIRDQLKESCSRMSIESGKALKDLASAIRTMTRPTFPNP 388
           QCAYR+ +LNSYL  + Q  + I+ +L E   RMS+ESGKA+K+++ +++ MT+P+  + 
Sbjct: 303 QCAYRIHALNSYLNADNQVSVDIKKKLGEPLRRMSLESGKAMKEMSISLKKMTKPSSSDL 362

Query: 389 HIEKSKAAAKNLKAALKIGPCDDGIDLLEIVPTATVASLLIDSISCIEKIAESVGELASL 419
           H++ +K+A K+L   L  G   + ++ LE+V   T  SLLID I+  EKI ES+ ELA+ 
Sbjct: 363 HVQNAKSACKSLTNLLNSGILKE-VEPLELVSLLTAISLLIDIINLTEKILESLHELATA 422

BLAST of Spg022624 vs. TAIR 10
Match: AT1G08430.1 (aluminum-activated malate transporter 1 )

HSP 1 Score: 391.0 bits (1003), Expect = 1.3e-108
Identity = 202/401 (50.37%), Postives = 284/401 (70.82%), Query Frame = 0

Query: 29  KVVELFGKTKKLAKDDPRRIVHSLKVGLAITLVSLFYYFE---PLYDGLGASAMWAVLTV 88
           KV E+  +  ++  +DPRRI+H+ KVGLA+ LVS FYY++   P  D  G +AMWAV+TV
Sbjct: 3   KVREIVREGIRVGNEDPRRIIHAFKVGLALVLVSSFYYYQPFGPFTDYFGINAMWAVMTV 62

Query: 89  VVVFEFSVGATLGRGLNRVLATFLAAALGFGAHFLADLAGDEAQPIMLACFVFFLAATTT 148
           VVVFEFSVGATLG+GLNR +AT +A  LG GAH LA L+G   +PI+L   VF  AA +T
Sbjct: 63  VVVFEFSVGATLGKGLNRGVATLVAGGLGIGAHQLARLSGATVEPILLVMLVFVQAALST 122

Query: 149 FVRFFPRIKARYDYGFLIFILTFCLVSVSGYRDDEILQVAYTRALTILIGTFIAVLICVF 208
           FVRFFP +K ++DYG LIFILTF L+S+SG+RD+EI+ +A +R  T++IG    +LI +F
Sbjct: 123 FVRFFPWVKTKFDYGILIFILTFALISLSGFRDEEIMDLAESRLSTVVIGGVSCILISIF 182

Query: 209 ICPVWAGHDLHSLLSNNIQLLANFLQDFGDRFSSSESKGD--------ELLQGCKSVLTS 268
           +CPVWAG DLHSLL++N   L++FLQDFGD +  +  KGD        + L+  KSVL S
Sbjct: 183 VCPVWAGQDLHSLLASNFDTLSHFLQDFGDEYFEAREKGDYKVVEKRKKNLERYKSVLDS 242

Query: 269 RQTEESLVNFARWEPRHGSFKFRHPWKQYRKIGSLTRQCAYRLESLNSYLLTEAQTPLQI 328
           +  EE+L N+A WEP HG F+FRHPWKQY  +G+L RQCAYR+++LNSY+ ++ Q P+ I
Sbjct: 243 KSDEEALANYAEWEPPHGQFRFRHPWKQYVAVGALLRQCAYRIDALNSYINSDFQIPVDI 302

Query: 329 RDQLKESCSRMSIESGKALKDLASAIRTMTRPTFPNPHIEKSKAAAKNLKAALKIGPCDD 388
           + +L+    RMS ESG ++K+++ +++ M + +  + H+  S+AA K+L   LK G  +D
Sbjct: 303 KKKLETPLRRMSSESGNSMKEMSISLKQMIKSSSSDIHVSNSQAACKSLSTLLKSGILND 362

Query: 389 GIDLLEIVPTATVASLLIDSISCIEKIAESVGELASLAHFK 419
            ++ L+++   T  S+LID ++  EKI+ESV ELAS A FK
Sbjct: 363 -VEPLQMISLMTTVSMLIDIVNLTEKISESVHELASAARFK 402

BLAST of Spg022624 vs. TAIR 10
Match: AT4G00910.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 310.5 bits (794), Expect = 2.3e-84
Identity = 179/432 (41.44%), Postives = 260/432 (60.19%), Query Frame = 0

Query: 29  KVVELFGKTKKLAKDDPRRIVHSLKVGLAITLVSLFYYFEPLYDGLGASAMWAVLTVVVV 88
           +V +   K  ++  DDP ++VH LKVGLA++LVS+FYY  PLYDG+G +AMWA++TVVVV
Sbjct: 48  RVAKFMRKAWRIGADDPAKVVHCLKVGLALSLVSIFYYMRPLYDGVGGNAMWAIMTVVVV 107

Query: 89  FEFSVGATLGRGLNRVLATFLAAALGFGAHFLADLAGDEAQPIMLACFVFFLAATTTFVR 148
           FE +VGAT  + +NRV+AT LA +LG   H++A  +G +A+  ++ C VF  A   T+ R
Sbjct: 108 FESNVGATFCKCVNRVVATILAGSLGIAVHWVATQSG-KAEVFVIGCSVFLFAFAATYSR 167

Query: 149 FFPRIKARYDYGFLIFILTFCLVSVSGYRDDEILQVAYTRALTILIGTFIAVLICVFICP 208
           F P  KAR+DYG +IFILTF LVSV GYR D+++++A  R  TI IGT I ++I VF CP
Sbjct: 168 FVPSFKARFDYGAMIFILTFSLVSVGGYRVDKLVELAQQRVSTIAIGTSICIIITVFFCP 227

Query: 209 VWAGHDLHSLLSNNIQLLANFL-----QDFGDRFSSSESKGDE----LLQGCKSVLTSRQ 268
           +WAG  LH L+  N++ LA+ L     + F +   S+    DE     LQG K VL S+ 
Sbjct: 228 IWAGSQLHRLIERNLEKLADSLDGCVAEYFKENEVSTNRNEDENTNMKLQGFKCVLNSKG 287

Query: 269 TEESL------------VNFARWEPRHGSFKFRHPWKQYRKIGSLTRQCAYRLESLNSYL 328
           TEE++             N ARWEP HGSF FRHPWK Y KIG+  R+CAY LE+L+  +
Sbjct: 288 TEEAMPLIRFSGFSFSQANLARWEPAHGSFNFRHPWKLYVKIGAAMRRCAYCLENLSICI 347

Query: 329 LTEAQTPLQIRDQLKESCSRMSIESGKALKDLASAIRTMTRPTFPNPHIEKSKAAAKNLK 388
             E + P Q+++   E+C ++S  S K L++LA  ++   + +  +  +    +A + L+
Sbjct: 348 NYETEAPDQVKNHFGEACMKLSSASSKILRELADMMKNTRKSSKMDFLVFDMNSAVQELQ 407

Query: 389 AALKIGPCD---------------------DGIDLLEIVPTATVASLLIDSISCIEKIAE 419
             LK  P +                       + L E++P AT+ SLLI++ + I+   E
Sbjct: 408 ETLKNVPIETNKPEEVPSEEENKVDSEERTTSMSLHEVLPVATLVSLLIENAARIQTAVE 467

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038880035.14.9e-21486.12aluminum-activated malate transporter 8-like [Benincasa hispida][more]
XP_023515628.19.3e-21386.03aluminum-activated malate transporter 2-like [Cucurbita pepo subsp. pepo][more]
XP_022960624.19.3e-21385.81aluminum-activated malate transporter 2-like [Cucurbita moschata][more]
XP_022141905.11.2e-21286.75aluminum-activated malate transporter 2-like [Momordica charantia][more]
XP_022988110.12.7e-21285.81aluminum-activated malate transporter 2-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9SJE82.9e-11651.39Aluminum-activated malate transporter 2 OS=Arabidopsis thaliana OX=3702 GN=ALMT2... [more]
Q9SRM98.1e-11152.39Aluminum-activated malate transporter 8 OS=Arabidopsis thaliana OX=3702 GN=ALMT8... [more]
Q9XIN12.0e-10949.29Aluminum-activated malate transporter 7 OS=Arabidopsis thaliana OX=3702 GN=ALMT7... [more]
Q9SJE91.9e-10750.37Aluminum-activated malate transporter 1 OS=Arabidopsis thaliana OX=3702 GN=ALMT1... [more]
Q76LB18.7e-9744.71Aluminum-activated malate transporter 1 OS=Triticum aestivum OX=4565 GN=ALMT1 PE... [more]
Match NameE-valueIdentityDescription
A0A6J1HBI74.5e-21385.81aluminum-activated malate transporter 2-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1CJE75.9e-21386.75aluminum-activated malate transporter 2-like OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1JIP21.3e-21285.81aluminum-activated malate transporter 2-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A5D3DX634.1e-20682.60Aluminum-activated malate transporter 2-like OS=Cucumis melo var. makuwa OX=1194... [more]
A0A1S3BNY94.1e-20682.60aluminum-activated malate transporter 2-like OS=Cucumis melo OX=3656 GN=LOC10349... [more]
Match NameE-valueIdentityDescription
AT1G08440.12.1e-11751.39Aluminium activated malate transporter family protein [more]
AT3G11680.15.8e-11252.39Aluminium activated malate transporter family protein [more]
AT2G27240.11.4e-11049.29Aluminium activated malate transporter family protein [more]
AT1G08430.11.3e-10850.37aluminum-activated malate transporter 1 [more]
AT4G00910.12.3e-8441.44Aluminium activated malate transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020966Aluminum-activated malate transporterPFAMPF11744ALMTcoord: 39..372
e-value: 1.0E-144
score: 482.9
NoneNo IPR availablePANTHERPTHR31086ALUMINUM-ACTIVATED MALATE TRANSPORTER 10coord: 20..425
NoneNo IPR availablePANTHERPTHR31086:SF53ALUMINUM ACTIVATED MALATE TRANSPORTER FAMILY PROTEINcoord: 20..425

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg022624.1Spg022624.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034220 ion transmembrane transport
biological_process GO:0015743 malate transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009705 plant-type vacuole membrane