Spg022595 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg022595
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Glucan endo-1,3-beta-glucosidase
Location	scaffold2: 13050050 .. 13052088 (+)
RNA-Seq Expression	Spg022595
Synteny	Spg022595

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAATGGCGATTGAAGATGTAGATTCCATGGCTCTTTTACCATTTGGATCTTCCTCTGGCACTGGCAGCTTCATTCGACTGCCTCAATGCGGAGGCCCTCGCCGGACAAGTACTCTTTCCCATTACATGTTTGGTTTTCTTTCTGCTCTGTTTTCCTCGTCTTGTTAATCCATTTCTATGATTTTACTTATGTGATCAATTTTTTGAGCTTTATGTTTCTCAATGTCATCCCTCGAAAACCCTCCACCAAGGATTTAGTGTTTTGAAATTCTGAATCTGCTAATAAACACGTATCTAAAGACATTGAAAACACGAAATACGATTTTGTTTTCTATTAATTCTCGTTTTTAGTTCACCAACCAACCATACCTGTCTTCCTGGTATTTTGGCATTCACGGCGAAATTCCATATTTGTATTCCTTCCACCTTTCCTTCTAAGATTGAAAACCATGACCTATATGCTTAAATGTTTGGATTGGGTTGCTATTTACCATGATATCATAGTAGTTGAATCTTTGCAATCTCATTTTCCCGCCGATTGATATTAATTGTTCATAGTAGTTAAATTTTTCTGATTTGTATATCACAATATTTATGAACATGCATGCTTCTAGTCAAACCTATGATGATAAAATGAGCCAAATTATCATCAACAATGTCGAAAAACTAGATGGATTTATTTGATTTGGGCTGGCTAAACAATTGGTTCTACTTGATTCTGTGTTTTCTGCCTTTCAAACTGATCAAATGTTACTTTTTTTGTGCATTCATATAGTCGCAACTGCCGAAGCCATCGGTGTTTGCTATGGAAGCAAGGGAGACAATTTACCAGAACCCAGAGAAGTAGTCGAACTATGCAAAAGCTATGGCATAAAGAGGATGAGGATTTATGATCCAAACAAAGCTATGTTAGACGCTCTTCGAGGATCAAACATAGAAGTGATTCTAGGCATGCCAAATACTGACATTGAAGGCATTGCTAATTTTTCTTTTGCAGCAAATTGGGTCCAGAGGAACATTCAAGCCTATGTACCGGACATCAAATTTCGATACATTGCTGTTGGAAATGAAGTGCGACCATCTGATTCTTTTGCTAGTTACGTTCTACCGGCCATGAGAAACATTTATAGTGCAATTGTGGCAGCCAATTTGCAGGATCATATGAATGTTTCCACAGTAATAACCACTGATTTGTTGGGGAGCAGCTTTCCTCCTTCTGTTGGATCATTTAGATCCGACGCTCGTGAATTTATAGAACCAATTATCAGCTTTCTGGCTAAGAATGGCTCACCCCTTCTTGCTAATGTGTATCCATACTACATTTATATCAGTAACCCCACATCAATTAACCTTGAATATGCTTTGTTCACAGCATCGCGACCTGTTATTGTAGATGGCAACTTAGAGTACTACAACTTTGTCAAAACAATAATCGATGCTCTTTATGCAGCTCTCGAGAAGTCAGGTGGAGCCAATGTGTCGATTGTGATATCAGAGAGCGGTTGGCCTTCAGGTACAGGCACCAATCCTGTAATTGAGAATGCAGTTGCTGAAACATACTATCAAAATTTGATCAGGTTTCTTCAAAGTGGGACTCCCAGAAGGCCTGAAAGAGCTATCGAAACGTATTTGTATGGTATGTTTGATGAAAATTTGATGTCTTCTGAAACGGCGAAACATTTTGGTTTATTCAAACTTGACAAAACCCCAAAGTACCGACTTAAGTTTAGTTAGGTTCCAAGCTCCACTCCTTATTTGGTGTTATATGGGATAATTAGTTGAGGTTTTAAAGTTTTAGTACTCGTTTGGAGTGACTTTTTAAGTGTTAGAAACAATTTTTTTTCACTTAAAAATACTTTTTTTAAACACATGAAGAGTCATTTCAAAAGGGTCTTTAATTGCTTTCAGGACATTATTGGATGCCCATGAATGGAACAATCCTGTGGTTGGGAATCAAAGATTACTCTCTTGGGTTTGGGATTTGGTCTTTAATCCTAGATATTTTATGTGATCGTCAAGCATTAACATTAGTGAGC

mRNA sequence

ATGGCAATGGCGATTGAAGATGTAGATTCCATGGCTCTTTTACCATTTGGATCTTCCTCTGGCACTGGCAGCTTCATTCGACTGCCTCAATGCGGAGGCCCTCGCCGGACAAGTACTCTTTCCCATTACATGTTTGTCGCAACTGCCGAAGCCATCGGTGTTTGCTATGGAAGCAAGGGAGACAATTTACCAGAACCCAGAGAAGTAGTCGAACTATGCAAAAGCTATGGCATAAAGAGGATGAGGATTTATGATCCAAACAAAGCTATGTTAGACGCTCTTCGAGGATCAAACATAGAAGTGATTCTAGGCATGCCAAATACTGACATTGAAGGCATTGCTAATTTTTCTTTTGCAGCAAATTGGGTCCAGAGGAACATTCAAGCCTATGTACCGGACATCAAATTTCGATACATTGCTGTTGGAAATGAAGTGCGACCATCTGATTCTTTTGCTAGTTACGTTCTACCGGCCATGAGAAACATTTATAGTGCAATTGTGGCAGCCAATTTGCAGGATCATATGAATGTTTCCACAGTAATAACCACTGATTTGTTGGGGAGCAGCTTTCCTCCTTCTGTTGGATCATTTAGATCCGACGCTCGTGAATTTATAGAACCAATTATCAGCTTTCTGGCTAAGAATGGCTCACCCCTTCTTGCTAATGTGTATCCATACTACATTTATATCAGTAACCCCACATCAATTAACCTTGAATATGCTTTGTTCACAGCATCGCGACCTGTTATTGTAGATGGCAACTTAGAGTACTACAACTTTGTCAAAACAATAATCGATGCTCTTTATGCAGCTCTCGAGAAGTCAGGTGGAGCCAATGTGTCGATTGTGATATCAGAGAGCGGTTGGCCTTCAGGTACAGGCACCAATCCTGTAATTGAGAATGCAGTTGCTGAAACATACTATCAAAATTTGATCAGGTTTCTTCAAAGTGGGACTCCCAGAAGGCCTGAAAGAGCTATCGAAACGTATTTGTATGGTATGTTTGATGAAAATTTGATGTCTTCTGAAACGGCGAAACATTTTGGTTTATTCAAACTTGACAAAACCCCAAAGTACCGACTTAAGTTTAGTTAG

Coding sequence (CDS)

ATGGCAATGGCGATTGAAGATGTAGATTCCATGGCTCTTTTACCATTTGGATCTTCCTCTGGCACTGGCAGCTTCATTCGACTGCCTCAATGCGGAGGCCCTCGCCGGACAAGTACTCTTTCCCATTACATGTTTGTCGCAACTGCCGAAGCCATCGGTGTTTGCTATGGAAGCAAGGGAGACAATTTACCAGAACCCAGAGAAGTAGTCGAACTATGCAAAAGCTATGGCATAAAGAGGATGAGGATTTATGATCCAAACAAAGCTATGTTAGACGCTCTTCGAGGATCAAACATAGAAGTGATTCTAGGCATGCCAAATACTGACATTGAAGGCATTGCTAATTTTTCTTTTGCAGCAAATTGGGTCCAGAGGAACATTCAAGCCTATGTACCGGACATCAAATTTCGATACATTGCTGTTGGAAATGAAGTGCGACCATCTGATTCTTTTGCTAGTTACGTTCTACCGGCCATGAGAAACATTTATAGTGCAATTGTGGCAGCCAATTTGCAGGATCATATGAATGTTTCCACAGTAATAACCACTGATTTGTTGGGGAGCAGCTTTCCTCCTTCTGTTGGATCATTTAGATCCGACGCTCGTGAATTTATAGAACCAATTATCAGCTTTCTGGCTAAGAATGGCTCACCCCTTCTTGCTAATGTGTATCCATACTACATTTATATCAGTAACCCCACATCAATTAACCTTGAATATGCTTTGTTCACAGCATCGCGACCTGTTATTGTAGATGGCAACTTAGAGTACTACAACTTTGTCAAAACAATAATCGATGCTCTTTATGCAGCTCTCGAGAAGTCAGGTGGAGCCAATGTGTCGATTGTGATATCAGAGAGCGGTTGGCCTTCAGGTACAGGCACCAATCCTGTAATTGAGAATGCAGTTGCTGAAACATACTATCAAAATTTGATCAGGTTTCTTCAAAGTGGGACTCCCAGAAGGCCTGAAAGAGCTATCGAAACGTATTTGTATGGTATGTTTGATGAAAATTTGATGTCTTCTGAAACGGCGAAACATTTTGGTTTATTCAAACTTGACAAAACCCCAAAGTACCGACTTAAGTTTAGTTAG

Protein sequence

MAMAIEDVDSMALLPFGSSSGTGSFIRLPQCGGPRRTSTLSHYMFVATAEAIGVCYGSKGDNLPEPREVVELCKSYGIKRMRIYDPNKAMLDALRGSNIEVILGMPNTDIEGIANFSFAANWVQRNIQAYVPDIKFRYIAVGNEVRPSDSFASYVLPAMRNIYSAIVAANLQDHMNVSTVITTDLLGSSFPPSVGSFRSDAREFIEPIISFLAKNGSPLLANVYPYYIYISNPTSINLEYALFTASRPVIVDGNLEYYNFVKTIIDALYAALEKSGGANVSIVISESGWPSGTGTNPVIENAVAETYYQNLIRFLQSGTPRRPERAIETYLYGMFDENLMSSETAKHFGLFKLDKTPKYRLKFS

Homology

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BLAST of Spg022595 vs. NCBI nr
Match: XP_022154376.1 (glucan endo-1,3-beta-glucosidase-like [Momordica charantia])

HSP 1 Score: 525.0 bits (1351), Expect = 5.1e-145
Identity = 262/365 (71.78%), Postives = 298/365 (81.64%), Query Frame = 0


Query: 1   MAMAIEDVDSMALLPFGSSSGTGSFIRLPQCGGPRRTSTLSHYMFVATAEAIGVCYGSKG 60
           MAMAI++VDSMALLP GS SG GSFIRLPQCGGPR T        V T +A+GVCY S G
Sbjct: 39  MAMAIDEVDSMALLPLGSVSGIGSFIRLPQCGGPRPT--------VETVQAVGVCYSSMG 98

Query: 61  DNLPEPREVVELCKSYGIKRMRIYDPNKAMLDALRGSNIEVILGMPNTDIEGIANFSFAA 120
           DNLPEP EVV+L KSYGIKRMRIYD NKA+L+ALRGSNIEV++G+PN D++ + N S A 
Sbjct: 99  DNLPEPGEVVQLYKSYGIKRMRIYDTNKAILNALRGSNIEVVVGIPNNDLQRVVNLSSAE 158

Query: 121 NWVQRNIQAYVPDIKFRYIAVGNEVRPSDSFASYVLPAMRNIYSAIVAANLQDHMNVSTV 180
           +WVQRNIQAY+P + FRY+ +GNEVRPSDSFA YVLPAMRNIYSAI AANLQD M VSTV
Sbjct: 159 SWVQRNIQAYIPHVNFRYVTIGNEVRPSDSFAGYVLPAMRNIYSAISAANLQDQMKVSTV 218

Query: 181 ITTDLLGSSFPPSVGSFRSDAREFIEPIISFLAKNGSPLLANVYPYYIYISNPTSINLEY 240
           I+T LLG+S+PPS GSFRSDAREFIEPI+SFLAKNGSPLLAN+YPYY YISN   I L+Y
Sbjct: 219 ISTVLLGASYPPSAGSFRSDAREFIEPIVSFLAKNGSPLLANLYPYYSYISNNRDITLDY 278

Query: 241 A-LFTASRPVIVDGNLEYYNFVKTIIDALYAALEKSGGANVSIVISESGWPSGTGTNPVI 300
           A L+  + P +VDGNL Y+N +   +DALYAALEKSGGAN SIVISESGWPSG GTN V+
Sbjct: 279 ALLYDYNFPTVVDGNLVYHNLLDATVDALYAALEKSGGANFSIVISESGWPSGAGTNTVV 338

Query: 301 ENAVAETYYQNLIRFLQSGTPRRPERAIETYLYGMFDENLMSSETAKHFGLFKLDKTPKY 360
           EN VA TYY+NLIR +QSG+PRRP RAIETYL+ MFDENL  SET KHFGLF  D   KY
Sbjct: 339 ENEVAGTYYRNLIRHVQSGSPRRPGRAIETYLFSMFDENLRPSETEKHFGLFTHDMKQKY 395

Query: 361 RLKFS 365
           +L  S
Sbjct: 399 QLSVS 395

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BLAST of Spg022595 vs. NCBI nr
Match: XP_022154361.1 (glucan endo-1,3-beta-glucosidase-like isoform X3 [Momordica charantia])

HSP 1 Score: 524.2 bits (1349), Expect = 8.7e-145
Identity = 262/364 (71.98%), Postives = 300/364 (82.42%), Query Frame = 0


Query: 1   MAMAIEDVDSMALLPFGSSSGTGSFIRLPQCGGPRRTSTLSHYMFVATAEAIGVCYGSKG 60
           +A+AIE+VDSMALLPFGS+SG GSF+RLPQCGGPR T        V T +AIGVCY +KG
Sbjct: 35  VALAIEEVDSMALLPFGSASGIGSFVRLPQCGGPRPT--------VETVQAIGVCYSTKG 94

Query: 61  DNLPEPREVVELCKSYGIKRMRIYDPNKAMLDALRGSNIEVILGMPNTDIEGIANFSFAA 120
           DNLPEP EVV L KS GIKRMRIYD ++ +L+ALRGSNIEV++G+PNTD+E +AN S AA
Sbjct: 95  DNLPEPGEVVRLYKSLGIKRMRIYDTDQTILNALRGSNIEVVVGIPNTDLERVANVSSAA 154

Query: 121 NWVQRNIQAYVPDIKFRYIAVGNEVRPSDSFASYVLPAMRNIYSAIVAANLQDHMNVSTV 180
           NWVQ NIQA+VP +KFRYI VGNEV+PSDSFA YVLPAMRNIYSAI AANLQD M +STV
Sbjct: 155 NWVQMNIQAFVPHVKFRYITVGNEVQPSDSFAGYVLPAMRNIYSAISAANLQDQMKISTV 214

Query: 181 ITTDLLGSSFPPSVGSFRSDAREFIEPIISFLAKNGSPLLANVYPYYIYISNPTSINLEY 240
           I+T LLG+S PPS GSF SDAREFIEPI+SFLAK GSPLLAN+Y YY YI     +NL+Y
Sbjct: 215 ISTALLGTSHPPSAGSFGSDAREFIEPIVSFLAKKGSPLLANLYTYYSYIGLSRQMNLDY 274

Query: 241 ALFTASRPVIVDGNLEYYNFVKTIIDALYAALEKSGGANVSIVISESGWPSGTGTNPVIE 300
           AL   S+ VI DGNLEY+N+V+++IDALYAALE SGGANVSIVISESGWPSG GTN +IE
Sbjct: 275 ALLKESQGVIADGNLEYHNYVESMIDALYAALENSGGANVSIVISESGWPSGAGTNTIIE 334

Query: 301 NAVAETYYQNLIRFLQSGTPRRPERAIETYLYGMFDENLMSSETAKHFGLFKLDKTPKYR 360
           + VAETYY NLIR +QSGTPRRP RAIETYL+ +FDENL  SE  KHFGLF  D  PKY+
Sbjct: 335 SVVAETYYVNLIRCVQSGTPRRPGRAIETYLFSIFDENLRPSEMEKHFGLFTHDMEPKYQ 390

Query: 361 LKFS 365
             FS
Sbjct: 395 FSFS 390

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BLAST of Spg022595 vs. NCBI nr
Match: XP_022154360.1 (glucan endo-1,3-beta-glucosidase-like isoform X2 [Momordica charantia])

HSP 1 Score: 469.5 bits (1207), Expect = 2.5e-128
Identity = 232/319 (72.73%), Postives = 265/319 (83.07%), Query Frame = 0


Query: 46  VATAEAIGVCYGSKGDNLPEPREVVELCKSYGIKRMRIYDPNKAMLDALRGSNIEVILGM 105
           V T +AIGVCY +KGDNLPEP EVV L KS GIKRMRIYD ++ +L+ALRGSNIEV++G+
Sbjct: 24  VETVQAIGVCYSTKGDNLPEPGEVVRLYKSLGIKRMRIYDTDQTILNALRGSNIEVVVGI 83

Query: 106 PNTDIEGIANFSFAANWVQRNIQAYVPDIKFRYIAVGNEVRPSDSFASYVLPAMRNIYSA 165
           PNTD+E +AN S AANWVQ NIQA+VP +KFRYI VGNEV+PSDSFA YVLPAMRNIYSA
Sbjct: 84  PNTDLERVANVSSAANWVQMNIQAFVPHVKFRYITVGNEVQPSDSFAGYVLPAMRNIYSA 143

Query: 166 IVAANLQDHMNVSTVITTDLLGSSFPPSVGSFRSDAREFIEPIISFLAKNGSPLLANVYP 225
           I AANLQD M +STVI+T LLG+S PPS GSF SDAREFIEPI+SFLAK GSPLLAN+Y 
Sbjct: 144 ISAANLQDQMKISTVISTALLGTSHPPSAGSFGSDAREFIEPIVSFLAKKGSPLLANLYT 203

Query: 226 YYIYISNPTSINLEYALFTASRPVIVDGNLEYYNFVKTIIDALYAALEKSGGANVSIVIS 285
           YY YI     +NL+YAL   S+ VI DGNLEY+N+V+++IDALYAALE SGGANVSIVIS
Sbjct: 204 YYSYIGLSRQMNLDYALLKESQGVIADGNLEYHNYVESMIDALYAALENSGGANVSIVIS 263

Query: 286 ESGWPSGTGTNPVIENAVAETYYQNLIRFLQSGTPRRPERAIETYLYGMFDENLMSSETA 345
           ESGWPSG GTN +IE+ VAETYY NLIR +QSGTPRRP RAIETYL+ +FDENL  SE  
Sbjct: 264 ESGWPSGAGTNTIIESVVAETYYVNLIRCVQSGTPRRPGRAIETYLFSIFDENLRPSEME 323

Query: 346 KHFGLFKLDKTPKYRLKFS 365
           KHFGLF  D  PKY+  FS
Sbjct: 324 KHFGLFTHDMEPKYQFSFS 342

[Close]

BLAST of Spg022595 vs. NCBI nr
Match: XP_022154359.1 (glucan endo-1,3-beta-glucosidase-like isoform X1 [Momordica charantia])

HSP 1 Score: 469.5 bits (1207), Expect = 2.5e-128
Identity = 242/364 (66.48%), Postives = 276/364 (75.82%), Query Frame = 0


Query: 1   MAMAIEDVDSMALLPFGSSSGTGSFIRLPQCGGPRRTSTLSHYMFVATAEAIGVCYGSKG 60
           +A+AIE+VDSMALLPFGS+SG GSF+RLPQCGGPR T        V T +AIGVCY +KG
Sbjct: 35  VALAIEEVDSMALLPFGSASGIGSFVRLPQCGGPRPT--------VETVQAIGVCYSTKG 94

Query: 61  DNLPEPREVVELCKSYGIKRMRIYDPNKAMLDALRGSNIEVILGMPNTDIEGIANFSFAA 120
           DNLPEP EVV L KS GIKRMRIYD ++ +L+ALRGSNIEV++G+PNTD+E +AN S AA
Sbjct: 95  DNLPEPGEVVRLYKSLGIKRMRIYDTDQTILNALRGSNIEVVVGIPNTDLERVANVSSAA 154

Query: 121 NWVQRNIQAYVPDIKFRYIAVGNEVRPSDSFASYVLPAMRNIYSAIVAANLQDHMNVSTV 180
           NWVQ NIQA+VP +KFRYI VGNEV+PSDSFA YVLPAMRNIYSAI AANLQD M +STV
Sbjct: 155 NWVQMNIQAFVPHVKFRYITVGNEVQPSDSFAGYVLPAMRNIYSAISAANLQDQMKISTV 214

Query: 181 ITTDLLGSSFPPSVGSFRSDAREFIEPIISFLAKNGSPLLANVYPYYIYISNPTSINLEY 240
           I+T LLG+S PPS GSF SDAREFIEPI+SFLAK                          
Sbjct: 215 ISTALLGTSHPPSAGSFGSDAREFIEPIVSFLAKK------------------------- 274

Query: 241 ALFTASRPVIVDGNLEYYNFVKTIIDALYAALEKSGGANVSIVISESGWPSGTGTNPVIE 300
                      DGNLEY+N+V+++IDALYAALE SGGANVSIVISESGWPSG GTN +IE
Sbjct: 275 -----------DGNLEYHNYVESMIDALYAALENSGGANVSIVISESGWPSGAGTNTIIE 334

Query: 301 NAVAETYYQNLIRFLQSGTPRRPERAIETYLYGMFDENLMSSETAKHFGLFKLDKTPKYR 360
           + VAETYY NLIR +QSGTPRRP RAIETYL+ +FDENL  SE  KHFGLF  D  PKY+
Sbjct: 335 SVVAETYYVNLIRCVQSGTPRRPGRAIETYLFSIFDENLRPSEMEKHFGLFTHDMEPKYQ 354

Query: 361 LKFS 365
             FS
Sbjct: 395 FSFS 354

[Close]

BLAST of Spg022595 vs. NCBI nr
Match: XP_022154372.1 (glucan endo-1,3-beta-glucosidase-like [Momordica charantia])

HSP 1 Score: 464.2 bits (1193), Expect = 1.1e-126
Identity = 237/366 (64.75%), Postives = 285/366 (77.87%), Query Frame = 0


Query: 1   MAMAIEDVDSMALLPFGSSSGTGSFIRLPQCGGPRRTSTLSHYMFVATAEAIGVCYGSKG 60
           MA+AIE+VDSMALLPFGS+SG  SF+RLPQCGGPR T           AE++GVCY S G
Sbjct: 37  MALAIEEVDSMALLPFGSASGIDSFVRLPQCGGPRPT----------VAESVGVCYASSG 96

Query: 61  DNLPEPREVVELCKSYGIKRMRIYDPNKAMLDALRGSNIEVILGMPNTDIEGIANFSFAA 120
            NLP   EVV+L K+  I +MRIY+P++A+L+AL+GSNIEV++G+PNTD+E + N S AA
Sbjct: 97  SNLPSATEVVQLYKNNSIGKMRIYNPSEAILNALQGSNIEVVVGIPNTDLEHVVNLSSAA 156

Query: 121 NWVQRNIQAYVPDIKFRYIAVGNEVRPSDSFASYVLPAMRNIYSAIVAANLQDHMNVSTV 180
           NWVQRNIQAY+  +KFRYIA+GN+V PSDSFA YVLPA+R+IY AI AANLQD + VSTV
Sbjct: 157 NWVQRNIQAYISHVKFRYIAIGNDVHPSDSFAGYVLPAIRSIYGAISAANLQDQIKVSTV 216

Query: 181 ITTDLL-GSSFPPSVGSFRSDAREFIEPIISFLAKNGSPLLANVYPYYIYISNPTSINLE 240
           I+  LL  SSFPPS+GSF S+AR FIEPI+SFLA+NGSPLLANVYPY+ Y     +I+L+
Sbjct: 217 ISISLLSNSSFPPSIGSFSSEARGFIEPIVSFLAENGSPLLANVYPYFTYTDIARTISLD 276

Query: 241 YALFTASRPVIV-DGNLEYYNFVKTIIDALYAALEKSGGANVSIVISESGWPSGTGTNPV 300
           YALF  S  + + DGN  Y +  ++++DALYAALEKSGG  VSIVISESGWPS  GT   
Sbjct: 277 YALFMQSPALAIRDGNFMYNSLFESMVDALYAALEKSGGNEVSIVISESGWPSSEGTAAT 336

Query: 301 IENAVAETYYQNLIRFLQSGTPRRPERAIETYLYGMFDENLMSSETAKHFGLFKLDKTPK 360
           IEN  A TYY+NLI F+QSGTPRRP RAIETYL+ MFDENL   E  KHFGLF  +K PK
Sbjct: 337 IEN--AGTYYRNLINFVQSGTPRRPGRAIETYLFSMFDENLKPPEIEKHFGLFTPNKKPK 390

Query: 361 YRLKFS 365
           Y+L FS
Sbjct: 397 YQLSFS 390

[Close]

BLAST of Spg022595 vs. ExPASy Swiss-Prot
Match: P07979 (Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3)

HSP 1 Score: 354.8 bits (909), Expect = 1.2e-96
Identity = 176/322 (54.66%), Postives = 231/322 (71.74%), Query Frame = 0


Query: 46  VATAEAIGVCYGSKGDNLPEPREVVELCKSYGIKRMRIYDPNKAMLDALRGSNIEVILGM 105
           +  A+++GVCYG  G+NLP   +VV+L KS  I+RMR+YDPN+A L ALRGSNIEV+LG+
Sbjct: 27  IVGAQSVGVCYGMLGNNLPPASQVVQLYKSKNIRRMRLYDPNQAALQALRGSNIEVMLGV 86

Query: 106 PNTDIEGI-ANFSFAANWVQRNIQAYVPDIKFRYIAVGNEVRP---SDSFASYVLPAMRN 165
           PN+D++ I AN S A NWVQRN++ + P +KFRYIAVGNEV P   + S   Y+LPAMRN
Sbjct: 87  PNSDLQNIAANPSNANNWVQRNVRNFWPAVKFRYIAVGNEVSPVTGTSSLTRYLLPAMRN 146

Query: 166 IYSAIVAANLQDHMNVSTVITTDLLGSSFPPSVGSFRSDAREFIEPIISFLAKNGSPLLA 225
           I +AI +A LQ+++ VS+ +   L+G+SFPPS GSFR+D R FI+PII F+ +  SPLL 
Sbjct: 147 IRNAISSAGLQNNIKVSSSVDMTLIGNSFPPSQGSFRNDVRSFIDPIIGFVRRINSPLLV 206

Query: 226 NVYPYYIYISNPTSINLEYALFTASRPVIVDGNLEYYNFVKTIIDALYAALEKSGGANVS 285
           N+YPY+ Y  NP  I+L YALFTA   V+ DG+L Y N    + DA+YAAL ++GG ++ 
Sbjct: 207 NIYPYFSYAGNPRDISLPYALFTAPNVVVQDGSLGYRNLFDAMSDAVYAALSRAGGGSIE 266

Query: 286 IVISESGWPSGTGTNPVIENAVAETYYQNLIRFLQSGTPRRPERAIETYLYGMFDENLMS 345
           IV+SESGWPS         NA   TYY+NLI+ ++ G+PRRP + IETYL+ MFDEN  +
Sbjct: 267 IVVSESGWPSAGAFAATTNNAA--TYYKNLIQHVKRGSPRRPNKVIETYLFAMFDENNKN 326

Query: 346 SETAKHFGLFKLDKTPKYRLKF 364
            E  KHFGLF  +K PKY L F
Sbjct: 327 PELEKHFGLFSPNKQPKYPLSF 346

[Close]

BLAST of Spg022595 vs. ExPASy Swiss-Prot
Match: A7PQW3 (Glucan endo-1,3-beta-glucosidase OS=Vitis vinifera OX=29760 GN=VIT_06s0061g00120 PE=1 SV=2)

HSP 1 Score: 354.0 bits (907), Expect = 2.0e-96
Identity = 181/321 (56.39%), Postives = 231/321 (71.96%), Query Frame = 0


Query: 49  AEAIGVCYGSKGDNLPEPREVVELCKSYGIKRMRIYDPNKAMLDALRGSNIEVILGMPNT 108
           A++IGVCYG  G+NLP   +V+ L KS GI  MRIYDPN   L ALRGS+IE+IL +PNT
Sbjct: 27  AQSIGVCYGRNGNNLPSASQVINLYKSNGIGSMRIYDPNSDTLQALRGSDIELILDVPNT 86

Query: 109 DIEGIA-NFSFAANWVQRNIQAYVPDIKFRYIAVGNEVRPSDS---FASYVLPAMRNIYS 168
           D++ +A + S AA WVQ N+  Y  ++KFRYIAVGNEV P+ S   +A YVLPAM+N+ S
Sbjct: 87  DLQSLASDASAAATWVQNNVVNYASEVKFRYIAVGNEVLPTGSNAQYAQYVLPAMKNVQS 146

Query: 169 AIVAANLQDHMNVSTVITTDLLGSSFPPSVGSFRSDAREFIEPIISFLAKNGSPLLANVY 228
           AI +A LQD + VST   + +LG S+PPS GSF  D   FI PIISFLA+NGSPLLAN+Y
Sbjct: 147 AITSAGLQDQIKVSTATFSAVLGKSYPPSEGSFSDDVSSFINPIISFLAENGSPLLANIY 206

Query: 229 PYYIYISNPTSINLEYALFTASRPVIVDGNLEYYNFVKTIIDALYAALEKSGGANVSIVI 288
           PY+ Y  +  +I L+YALFTAS  V+ DG+ +Y N    ++DALYAALEK+GG+N+ IV+
Sbjct: 207 PYFSYTGDTQNIRLDYALFTASGVVVQDGSYQYQNLFDALLDALYAALEKAGGSNLKIVV 266

Query: 289 SESGWPSGTGTNPVIENAVAETYYQNLIRFLQSGTPRRPERAIETYLYGMFDENLMSS-E 348
           SESGWPS  GT   ++N  A TYY+NLI  ++ GTPR+   AIETYL+ MFDEN  +  E
Sbjct: 267 SESGWPSEGGTAATVDN--ARTYYKNLINHVKGGTPRK-SGAIETYLFAMFDENQKTGLE 326

Query: 349 TAKHFGLFKLDKTPKYRLKFS 365
           T KHFGLF   +  KY++ FS
Sbjct: 327 TEKHFGLFTPGQESKYQISFS 344

[Close]

BLAST of Spg022595 vs. ExPASy Swiss-Prot
Match: O22317 (Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2)

HSP 1 Score: 345.9 bits (886), Expect = 5.6e-94
Identity = 169/316 (53.48%), Postives = 224/316 (70.89%), Query Frame = 0


Query: 50  EAIGVCYGSKGDNLPEPREVVELCKSYGIKRMRIYDPNKAMLDALRGSNIEVILGMPNTD 109
           ++IGVCYG  G+NLP P EVV L KS  I RMR+YDPN+A L ALR SNI+V+L +P +D
Sbjct: 27  QSIGVCYGMLGNNLPPPSEVVSLYKSNNIARMRLYDPNQAALQALRNSNIQVLLDVPRSD 86

Query: 110 IEGIA-NFSFAANWVQRNIQAYVPDIKFRYIAVGNEVRPSDSFASYVLPAMRNIYSAIVA 169
           ++ +A N S A +W++RN+ AY P + FRYIAVGNE+ P    A Y+LPAMRNIY+A+ +
Sbjct: 87  VQSLASNPSAAGDWIRRNVVAYWPSVSFRYIAVGNELIPGSDLAQYILPAMRNIYNALSS 146

Query: 170 ANLQDHMNVSTVITTDLLGSSFPPSVGSFRSDAREFIEPIISFLAKNGSPLLANVYPYYI 229
           A LQ+ + VST + T +LG+S+PPS G+F S A+ ++ PI+ FLA NG+PLL NVYPY+ 
Sbjct: 147 AGLQNQIKVSTAVDTGVLGTSYPPSAGAFSSAAQAYLSPIVQFLASNGAPLLVNVYPYFS 206

Query: 230 YISNPTSINLEYALFTASRPVIVDGNLEYYNFVKTIIDALYAALEKSGGANVSIVISESG 289
           Y  NP  I+L YALFTAS  V+ DG   Y N    I+DA++AALE+ GGANV++V+SESG
Sbjct: 207 YTGNPGQISLPYALFTASGVVVQDGRFSYQNLFDAIVDAVFAALERVGGANVAVVVSESG 266

Query: 290 WPS-GTGTNPVIENAVAETYYQNLIRFLQSGTPRRPERAIETYLYGMFDENLMSSETAKH 349
           WPS G G      N  A TY QNLIR +  GTPRRP + IE Y++ MF+EN  +    ++
Sbjct: 267 WPSAGGGAEASTSN--ARTYNQNLIRHVGGGTPRRPGKEIEAYIFEMFNENQKAGGIEQN 326

Query: 350 FGLFKLDKTPKYRLKF 364
           FGLF  +K P Y++ F
Sbjct: 327 FGLFYPNKQPVYQISF 340

[Close]

BLAST of Spg022595 vs. ExPASy Swiss-Prot
Match: P52407 (Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform OS=Hevea brasiliensis OX=3981 GN=HGN1 PE=1 SV=2)

HSP 1 Score: 344.4 bits (882), Expect = 1.6e-93
Identity = 177/356 (49.72%), Postives = 233/356 (65.45%), Query Frame = 0


Query: 18  SSSGTGSFIRLPQCGGPRRTSTLSHYMFVA-----TAEAIGVCYGSKGDNLPEPREVVEL 77
           S+SGT S         P RT+ +    F A     T   +GVCYG +G+NLP   EV+ L
Sbjct: 6   STSGTSSSF-------PSRTTVMLLLFFFAASVGITDAQVGVCYGMQGNNLPPVSEVIAL 65

Query: 78  CKSYGIKRMRIYDPNKAMLDALRGSNIEVILGMPNTDIEGIANFSFAANWVQRNIQAYVP 137
            K   I RMRIYDPN+A+L+ALRGSNIE+ILG+PN+D++ + N S A +WVQ+N++ +  
Sbjct: 66  YKKSNITRMRIYDPNRAVLEALRGSNIELILGVPNSDLQSLTNPSNAKSWVQKNVRGFWS 125

Query: 138 DIKFRYIAVGNEVRPSDS----FASYVLPAMRNIYSAIVAANLQDHMNVSTVITTDLLGS 197
            + FRYIAVGNE+ P +      A +VLPAMRNI+ AI +A LQD + VST I   L+G+
Sbjct: 126 SVLFRYIAVGNEISPVNRGTAWLAQFVLPAMRNIHDAIRSAGLQDQIKVSTAIDLTLVGN 185

Query: 198 SFPPSVGSFRSDAREFIEPIISFLAKNGSPLLANVYPYYIYISNPTSINLEYALFTASRP 257
           S+PPS G+FR D R +++PII FL+   SPLLAN+YPY+ Y  NP  I+L YALFT+   
Sbjct: 186 SYPPSAGAFRDDVRSYLDPIIGFLSSIRSPLLANIYPYFTYAYNPRDISLPYALFTSPSV 245

Query: 258 VIVDGNLEYYNFVKTIIDALYAALEKSGGANVSIVISESGWPSGTGTNPVIENAVAETYY 317
           V+ DG   Y N     +DALY+ALE++ G ++ +V+SESGWPS        +N    TY 
Sbjct: 246 VVWDGQRGYKNLFDATLDALYSALERASGGSLEVVVSESGWPSAGAFAATFDN--GRTYL 305

Query: 318 QNLIRFLQSGTPRRPERAIETYLYGMFDENLMSSETAKHFGLFKLDKTPKYRLKFS 365
            NLI+ ++ GTP+RP RAIETYL+ MFDEN    E  KHFGLF  +K  KY L FS
Sbjct: 306 SNLIQHVKGGTPKRPNRAIETYLFAMFDENKKQPEVEKHFGLFFPNKWQKYNLNFS 352

[Close]

BLAST of Spg022595 vs. ExPASy Swiss-Prot
Match: P52408 (Glucan endo-1,3-beta-glucosidase, basic isoform OS=Prunus persica OX=3760 GN=GNS1 PE=3 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 7.5e-91
Identity = 168/318 (52.83%), Postives = 220/318 (69.18%), Query Frame = 0


Query: 48  TAEAIGVCYGSKGDNLPEPREVVELCKSYGIKRMRIYDPNKAMLDALRGSNIEVILGMPN 107
           T   IGVC G  GD+LP   EVV L K+  I RMR+YDPN A L+ALRGSNI+++LG+PN
Sbjct: 36  TGAPIGVCNGMVGDDLPPQAEVVALYKTNNIPRMRLYDPNPAALEALRGSNIKLLLGVPN 95

Query: 108 TDIEGIANFSFAAN-WVQRNIQAYVPDIKFRYIAVGNEVRPSDSFASYVLPAMRNIYSAI 167
            +++ IA     AN WVQ N++ Y  ++KF+YIAVGNEV+PSDSFA +++PAMRNI  AI
Sbjct: 96  ENLQYIALSQANANAWVQNNVRNYA-NVKFKYIAVGNEVKPSDSFAQFLVPAMRNIQEAI 155

Query: 168 VAANLQDHMNVSTVITTDLLGSSFPPSVGSFRSDAREFIEPIISFLAKNGSPLLANVYPY 227
             A L   + VST I T +LG +FPPS+GSF+S+    + PII FL  + SPLL N+YPY
Sbjct: 156 SLAGLAKKIKVSTAIDTGVLGETFPPSIGSFKSEYNALLYPIIRFLVSHQSPLLVNLYPY 215

Query: 228 YIYISNPTSINLEYALFTASRPVIVDGNLEYYNFVKTIIDALYAALEKSGGANVSIVISE 287
           + Y  N   I L+YALFTA   V+ DGN  Y N    ++D +YAALEK+GG ++ +VISE
Sbjct: 216 FAYSGNTQDIRLDYALFTAPSVVVQDGNFGYRNLFDAMLDGVYAALEKAGGGSLKVVISE 275

Query: 288 SGWPSGTGTNPVIENAVAETYYQNLIRFLQSGTPRRPERAIETYLYGMFDENLMSSETAK 347
           +GWPS  GT   I+N  A T+  NLI+ ++ GTPRRP R IETY++ MFDEN  + E  K
Sbjct: 276 TGWPSAAGTATTIDN--ARTFISNLIQHVKEGTPRRPGRPIETYIFAMFDENRKTPELEK 335

Query: 348 HFGLFKLDKTPKYRLKFS 365
           H+GLF   K PKY++ F+
Sbjct: 336 HWGLFSPTKQPKYQISFN 350

[Close]

BLAST of Spg022595 vs. ExPASy TrEMBL
Match: A0A6J1DNI9 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111021658 PE=3 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 2.5e-145
Identity = 262/365 (71.78%), Postives = 298/365 (81.64%), Query Frame = 0


Query: 1   MAMAIEDVDSMALLPFGSSSGTGSFIRLPQCGGPRRTSTLSHYMFVATAEAIGVCYGSKG 60
           MAMAI++VDSMALLP GS SG GSFIRLPQCGGPR T        V T +A+GVCY S G
Sbjct: 39  MAMAIDEVDSMALLPLGSVSGIGSFIRLPQCGGPRPT--------VETVQAVGVCYSSMG 98

Query: 61  DNLPEPREVVELCKSYGIKRMRIYDPNKAMLDALRGSNIEVILGMPNTDIEGIANFSFAA 120
           DNLPEP EVV+L KSYGIKRMRIYD NKA+L+ALRGSNIEV++G+PN D++ + N S A 
Sbjct: 99  DNLPEPGEVVQLYKSYGIKRMRIYDTNKAILNALRGSNIEVVVGIPNNDLQRVVNLSSAE 158

Query: 121 NWVQRNIQAYVPDIKFRYIAVGNEVRPSDSFASYVLPAMRNIYSAIVAANLQDHMNVSTV 180
           +WVQRNIQAY+P + FRY+ +GNEVRPSDSFA YVLPAMRNIYSAI AANLQD M VSTV
Sbjct: 159 SWVQRNIQAYIPHVNFRYVTIGNEVRPSDSFAGYVLPAMRNIYSAISAANLQDQMKVSTV 218

Query: 181 ITTDLLGSSFPPSVGSFRSDAREFIEPIISFLAKNGSPLLANVYPYYIYISNPTSINLEY 240
           I+T LLG+S+PPS GSFRSDAREFIEPI+SFLAKNGSPLLAN+YPYY YISN   I L+Y
Sbjct: 219 ISTVLLGASYPPSAGSFRSDAREFIEPIVSFLAKNGSPLLANLYPYYSYISNNRDITLDY 278

Query: 241 A-LFTASRPVIVDGNLEYYNFVKTIIDALYAALEKSGGANVSIVISESGWPSGTGTNPVI 300
           A L+  + P +VDGNL Y+N +   +DALYAALEKSGGAN SIVISESGWPSG GTN V+
Sbjct: 279 ALLYDYNFPTVVDGNLVYHNLLDATVDALYAALEKSGGANFSIVISESGWPSGAGTNTVV 338

Query: 301 ENAVAETYYQNLIRFLQSGTPRRPERAIETYLYGMFDENLMSSETAKHFGLFKLDKTPKY 360
           EN VA TYY+NLIR +QSG+PRRP RAIETYL+ MFDENL  SET KHFGLF  D   KY
Sbjct: 339 ENEVAGTYYRNLIRHVQSGSPRRPGRAIETYLFSMFDENLRPSETEKHFGLFTHDMKQKY 395

Query: 361 RLKFS 365
           +L  S
Sbjct: 399 QLSVS 395

[Close]

BLAST of Spg022595 vs. ExPASy TrEMBL
Match: A0A6J1DNH1 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111021643 PE=3 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 4.2e-145
Identity = 262/364 (71.98%), Postives = 300/364 (82.42%), Query Frame = 0


Query: 1   MAMAIEDVDSMALLPFGSSSGTGSFIRLPQCGGPRRTSTLSHYMFVATAEAIGVCYGSKG 60
           +A+AIE+VDSMALLPFGS+SG GSF+RLPQCGGPR T        V T +AIGVCY +KG
Sbjct: 35  VALAIEEVDSMALLPFGSASGIGSFVRLPQCGGPRPT--------VETVQAIGVCYSTKG 94

Query: 61  DNLPEPREVVELCKSYGIKRMRIYDPNKAMLDALRGSNIEVILGMPNTDIEGIANFSFAA 120
           DNLPEP EVV L KS GIKRMRIYD ++ +L+ALRGSNIEV++G+PNTD+E +AN S AA
Sbjct: 95  DNLPEPGEVVRLYKSLGIKRMRIYDTDQTILNALRGSNIEVVVGIPNTDLERVANVSSAA 154

Query: 121 NWVQRNIQAYVPDIKFRYIAVGNEVRPSDSFASYVLPAMRNIYSAIVAANLQDHMNVSTV 180
           NWVQ NIQA+VP +KFRYI VGNEV+PSDSFA YVLPAMRNIYSAI AANLQD M +STV
Sbjct: 155 NWVQMNIQAFVPHVKFRYITVGNEVQPSDSFAGYVLPAMRNIYSAISAANLQDQMKISTV 214

Query: 181 ITTDLLGSSFPPSVGSFRSDAREFIEPIISFLAKNGSPLLANVYPYYIYISNPTSINLEY 240
           I+T LLG+S PPS GSF SDAREFIEPI+SFLAK GSPLLAN+Y YY YI     +NL+Y
Sbjct: 215 ISTALLGTSHPPSAGSFGSDAREFIEPIVSFLAKKGSPLLANLYTYYSYIGLSRQMNLDY 274

Query: 241 ALFTASRPVIVDGNLEYYNFVKTIIDALYAALEKSGGANVSIVISESGWPSGTGTNPVIE 300
           AL   S+ VI DGNLEY+N+V+++IDALYAALE SGGANVSIVISESGWPSG GTN +IE
Sbjct: 275 ALLKESQGVIADGNLEYHNYVESMIDALYAALENSGGANVSIVISESGWPSGAGTNTIIE 334

Query: 301 NAVAETYYQNLIRFLQSGTPRRPERAIETYLYGMFDENLMSSETAKHFGLFKLDKTPKYR 360
           + VAETYY NLIR +QSGTPRRP RAIETYL+ +FDENL  SE  KHFGLF  D  PKY+
Sbjct: 335 SVVAETYYVNLIRCVQSGTPRRPGRAIETYLFSIFDENLRPSEMEKHFGLFTHDMEPKYQ 390

Query: 361 LKFS 365
             FS
Sbjct: 395 FSFS 390

[Close]

BLAST of Spg022595 vs. ExPASy TrEMBL
Match: A0A6J1DLU4 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111021643 PE=3 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 1.2e-128
Identity = 242/364 (66.48%), Postives = 276/364 (75.82%), Query Frame = 0


Query: 1   MAMAIEDVDSMALLPFGSSSGTGSFIRLPQCGGPRRTSTLSHYMFVATAEAIGVCYGSKG 60
           +A+AIE+VDSMALLPFGS+SG GSF+RLPQCGGPR T        V T +AIGVCY +KG
Sbjct: 35  VALAIEEVDSMALLPFGSASGIGSFVRLPQCGGPRPT--------VETVQAIGVCYSTKG 94

Query: 61  DNLPEPREVVELCKSYGIKRMRIYDPNKAMLDALRGSNIEVILGMPNTDIEGIANFSFAA 120
           DNLPEP EVV L KS GIKRMRIYD ++ +L+ALRGSNIEV++G+PNTD+E +AN S AA
Sbjct: 95  DNLPEPGEVVRLYKSLGIKRMRIYDTDQTILNALRGSNIEVVVGIPNTDLERVANVSSAA 154

Query: 121 NWVQRNIQAYVPDIKFRYIAVGNEVRPSDSFASYVLPAMRNIYSAIVAANLQDHMNVSTV 180
           NWVQ NIQA+VP +KFRYI VGNEV+PSDSFA YVLPAMRNIYSAI AANLQD M +STV
Sbjct: 155 NWVQMNIQAFVPHVKFRYITVGNEVQPSDSFAGYVLPAMRNIYSAISAANLQDQMKISTV 214

Query: 181 ITTDLLGSSFPPSVGSFRSDAREFIEPIISFLAKNGSPLLANVYPYYIYISNPTSINLEY 240
           I+T LLG+S PPS GSF SDAREFIEPI+SFLAK                          
Sbjct: 215 ISTALLGTSHPPSAGSFGSDAREFIEPIVSFLAKK------------------------- 274

Query: 241 ALFTASRPVIVDGNLEYYNFVKTIIDALYAALEKSGGANVSIVISESGWPSGTGTNPVIE 300
                      DGNLEY+N+V+++IDALYAALE SGGANVSIVISESGWPSG GTN +IE
Sbjct: 275 -----------DGNLEYHNYVESMIDALYAALENSGGANVSIVISESGWPSGAGTNTIIE 334

Query: 301 NAVAETYYQNLIRFLQSGTPRRPERAIETYLYGMFDENLMSSETAKHFGLFKLDKTPKYR 360
           + VAETYY NLIR +QSGTPRRP RAIETYL+ +FDENL  SE  KHFGLF  D  PKY+
Sbjct: 335 SVVAETYYVNLIRCVQSGTPRRPGRAIETYLFSIFDENLRPSEMEKHFGLFTHDMEPKYQ 354

Query: 361 LKFS 365
             FS
Sbjct: 395 FSFS 354

[Close]

BLAST of Spg022595 vs. ExPASy TrEMBL
Match: A0A6J1DJE2 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111021643 PE=3 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 1.2e-128
Identity = 232/319 (72.73%), Postives = 265/319 (83.07%), Query Frame = 0


Query: 46  VATAEAIGVCYGSKGDNLPEPREVVELCKSYGIKRMRIYDPNKAMLDALRGSNIEVILGM 105
           V T +AIGVCY +KGDNLPEP EVV L KS GIKRMRIYD ++ +L+ALRGSNIEV++G+
Sbjct: 24  VETVQAIGVCYSTKGDNLPEPGEVVRLYKSLGIKRMRIYDTDQTILNALRGSNIEVVVGI 83

Query: 106 PNTDIEGIANFSFAANWVQRNIQAYVPDIKFRYIAVGNEVRPSDSFASYVLPAMRNIYSA 165
           PNTD+E +AN S AANWVQ NIQA+VP +KFRYI VGNEV+PSDSFA YVLPAMRNIYSA
Sbjct: 84  PNTDLERVANVSSAANWVQMNIQAFVPHVKFRYITVGNEVQPSDSFAGYVLPAMRNIYSA 143

Query: 166 IVAANLQDHMNVSTVITTDLLGSSFPPSVGSFRSDAREFIEPIISFLAKNGSPLLANVYP 225
           I AANLQD M +STVI+T LLG+S PPS GSF SDAREFIEPI+SFLAK GSPLLAN+Y 
Sbjct: 144 ISAANLQDQMKISTVISTALLGTSHPPSAGSFGSDAREFIEPIVSFLAKKGSPLLANLYT 203

Query: 226 YYIYISNPTSINLEYALFTASRPVIVDGNLEYYNFVKTIIDALYAALEKSGGANVSIVIS 285
           YY YI     +NL+YAL   S+ VI DGNLEY+N+V+++IDALYAALE SGGANVSIVIS
Sbjct: 204 YYSYIGLSRQMNLDYALLKESQGVIADGNLEYHNYVESMIDALYAALENSGGANVSIVIS 263

Query: 286 ESGWPSGTGTNPVIENAVAETYYQNLIRFLQSGTPRRPERAIETYLYGMFDENLMSSETA 345
           ESGWPSG GTN +IE+ VAETYY NLIR +QSGTPRRP RAIETYL+ +FDENL  SE  
Sbjct: 264 ESGWPSGAGTNTIIESVVAETYYVNLIRCVQSGTPRRPGRAIETYLFSIFDENLRPSEME 323

Query: 346 KHFGLFKLDKTPKYRLKFS 365
           KHFGLF  D  PKY+  FS
Sbjct: 324 KHFGLFTHDMEPKYQFSFS 342

[Close]

BLAST of Spg022595 vs. ExPASy TrEMBL
Match: A0A6J1DLI3 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111021653 PE=3 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 5.2e-127
Identity = 237/366 (64.75%), Postives = 285/366 (77.87%), Query Frame = 0


Query: 1   MAMAIEDVDSMALLPFGSSSGTGSFIRLPQCGGPRRTSTLSHYMFVATAEAIGVCYGSKG 60
           MA+AIE+VDSMALLPFGS+SG  SF+RLPQCGGPR T           AE++GVCY S G
Sbjct: 37  MALAIEEVDSMALLPFGSASGIDSFVRLPQCGGPRPT----------VAESVGVCYASSG 96

Query: 61  DNLPEPREVVELCKSYGIKRMRIYDPNKAMLDALRGSNIEVILGMPNTDIEGIANFSFAA 120
            NLP   EVV+L K+  I +MRIY+P++A+L+AL+GSNIEV++G+PNTD+E + N S AA
Sbjct: 97  SNLPSATEVVQLYKNNSIGKMRIYNPSEAILNALQGSNIEVVVGIPNTDLEHVVNLSSAA 156

Query: 121 NWVQRNIQAYVPDIKFRYIAVGNEVRPSDSFASYVLPAMRNIYSAIVAANLQDHMNVSTV 180
           NWVQRNIQAY+  +KFRYIA+GN+V PSDSFA YVLPA+R+IY AI AANLQD + VSTV
Sbjct: 157 NWVQRNIQAYISHVKFRYIAIGNDVHPSDSFAGYVLPAIRSIYGAISAANLQDQIKVSTV 216

Query: 181 ITTDLL-GSSFPPSVGSFRSDAREFIEPIISFLAKNGSPLLANVYPYYIYISNPTSINLE 240
           I+  LL  SSFPPS+GSF S+AR FIEPI+SFLA+NGSPLLANVYPY+ Y     +I+L+
Sbjct: 217 ISISLLSNSSFPPSIGSFSSEARGFIEPIVSFLAENGSPLLANVYPYFTYTDIARTISLD 276

Query: 241 YALFTASRPVIV-DGNLEYYNFVKTIIDALYAALEKSGGANVSIVISESGWPSGTGTNPV 300
           YALF  S  + + DGN  Y +  ++++DALYAALEKSGG  VSIVISESGWPS  GT   
Sbjct: 277 YALFMQSPALAIRDGNFMYNSLFESMVDALYAALEKSGGNEVSIVISESGWPSSEGTAAT 336

Query: 301 IENAVAETYYQNLIRFLQSGTPRRPERAIETYLYGMFDENLMSSETAKHFGLFKLDKTPK 360
           IEN  A TYY+NLI F+QSGTPRRP RAIETYL+ MFDENL   E  KHFGLF  +K PK
Sbjct: 337 IEN--AGTYYRNLINFVQSGTPRRPGRAIETYLFSMFDENLKPPEIEKHFGLFTPNKKPK 390

Query: 361 YRLKFS 365
           Y+L FS
Sbjct: 397 YQLSFS 390

[Close]

BLAST of Spg022595 vs. TAIR 10
Match: AT4G16260.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 328.6 bits (841), Expect = 6.5e-90
Identity = 158/319 (49.53%), Postives = 217/319 (68.03%), Query Frame = 0


Query: 48  TAEAIGVCYGSKGDNLPEPREVVELCKSYGIKRMRIYDPNKAMLDALRGSNIEVILGMPN 107
           + E++GVCYG  G+NLP   + + L +   I+R+R+YDPN+A L+ALR + IEVI+G+PN
Sbjct: 20  SGESVGVCYGMMGNNLPSQSDTIALFRQNNIRRVRLYDPNQAALNALRNTGIEVIIGVPN 79

Query: 108 TDIEGIANFSFAANWVQRNIQAYVPDIKFRYIAVGNEVRPSDSFASYVLPAMRNIYSAIV 167
           TD+  + N S A +W+Q N+  Y P + F+YIAVGNEV PS+     VLPAMRN+Y A+ 
Sbjct: 80  TDLRSLTNPSSARSWLQNNVLNYYPAVSFKYIAVGNEVSPSNG-GDVVLPAMRNVYDALR 139

Query: 168 AANLQDHMNVSTVITTDLLGSSFPPSVGSFRSDAREFIEPIISFLAKNGSPLLANVYPYY 227
            ANLQD + VST I   L+G+SFPPS G FR D R +I+P+I FL    S LLAN+YPY+
Sbjct: 140 GANLQDRIKVSTAIDMTLIGNSFPPSSGEFRGDVRWYIDPVIGFLTSTNSALLANIYPYF 199

Query: 228 IYISNPTSINLEYALFTASRPVIVDGNLEYYNFVKTIIDALYAALEKSGGANVSIVISES 287
            Y+ NP  I+L YALFT+   V+ DG+  Y N    ++D +Y+A+E+SGG ++ +V+SES
Sbjct: 200 SYVDNPRDISLSYALFTSPSVVVWDGSRGYQNLFDALLDVVYSAVERSGGGSLPVVVSES 259

Query: 288 GWPSGTGTNPVIENAVAETYYQNLIRFLQS--GTPRRPERAIETYLYGMFDENLMSSETA 347
           GWPS  G     +NA A  +Y NL   ++   GTP+RP R +ETYL+ MFDEN  S E  
Sbjct: 260 GWPSNGGNAASFDNARA--FYTNLASRVRENRGTPKRPGRGVETYLFAMFDENQKSPEIE 319

Query: 348 KHFGLFKLDKTPKYRLKFS 365
           K+FGLF  +K PK+ + FS
Sbjct: 320 KNFGLFFPNKQPKFPITFS 335

[Close]

BLAST of Spg022595 vs. TAIR 10
Match: AT3G57270.1 (beta-1,3-glucanase 1 )

HSP 1 Score: 300.4 bits (768), Expect = 1.9e-81
Identity = 151/319 (47.34%), Postives = 210/319 (65.83%), Query Frame = 0


Query: 48  TAEAIGVCYGSKGDNLPEPREVVELCKSYGIKRMRIYDPNKAMLDALRGSNIEVILGMPN 107
           T   +GVCYG  G+NLP P E + L K   I+R+R+Y P+  +L ALRGSNIEV LG+PN
Sbjct: 23  TGGQVGVCYGRNGNNLPSPAETIALFKQKNIQRVRLYSPDHDVLAALRGSNIEVTLGLPN 82

Query: 108 TDIEGIANFSFAAN-WVQRNIQAYVPDIKFRYIAVGNEVRPSDSFASYVLPAMRNIYSAI 167
           + ++ +A+    AN WVQ  +  Y   ++FRYI+VGNEV+ SDS+A +++PAM NI  A+
Sbjct: 83  SYLQSVASSQSQANAWVQTYVMNYANGVRFRYISVGNEVKISDSYAQFLVPAMENIDRAV 142

Query: 168 VAANLQDHMNVSTVITTDLLGSSFPPSVGSFRSDAREFIEPIISFLAKNGSPLLANVYPY 227
           +AA L   + VST +   +L  S+PPS GSFR D    +EPII FL    SPLL N+Y Y
Sbjct: 143 LAAGLGGRIKVSTSVDMGVLRESYPPSKGSFRGDVMVVMEPIIRFLVSKNSPLLLNLYTY 202

Query: 228 YIYISNPTSINLEYALFTASRPVIVDGNLEYYNFVKTIIDALYAALEKSGGANVSIVISE 287
           + Y  N   I L+YALFTA   ++ D    Y N    ++DA+Y+ALEKSGGA++ IV++E
Sbjct: 203 FSYAGNVGQIRLDYALFTAPSGIVSDPPRSYQNLFDAMLDAMYSALEKSGGASLEIVVAE 262

Query: 288 SGWPSGTGTNPVIENAVAETYYQNLIRFLQSGTPRRPERAIETYLYGMFDENLMSSE--T 347
           +GWP+G GT+  IEN  A  Y  NLI+ +++GTP+RP + IETYL+ ++DEN   +    
Sbjct: 263 TGWPTGGGTDTNIEN--ARIYNNNLIKHVKNGTPKRPGKEIETYLFAIYDENQKPTPPYV 322

Query: 348 AKHFGLFKLDKTPKYRLKF 364
            K +GLF  +K PKY + F
Sbjct: 323 EKFWGLFYPNKQPKYDINF 339

[Close]

BLAST of Spg022595 vs. TAIR 10
Match: AT3G57260.1 (beta-1,3-glucanase 2 )

HSP 1 Score: 294.7 bits (753), Expect = 1.0e-79
Identity = 152/321 (47.35%), Postives = 211/321 (65.73%), Query Frame = 0


Query: 45  FVATAEAIGVCYGSKGDNLPEPREVVELCKSYGIKRMRIYDPNKAMLDALRGSNIEVILG 104
           F  TA  IGVCYG  GD LP P +VV L K   I+RMR+Y P+   L ALRGS+IE+IL 
Sbjct: 25  FNHTAGQIGVCYGMLGDTLPSPSDVVALYKQQNIQRMRLYGPDPGALAALRGSDIELILD 84

Query: 105 MPNTDIEGIANFSFAAN-WVQRNIQAYVPDIKFRYIAVGNEVRPSDSFASYVLPAMRNIY 164
           +P++D+E +A+    A+ WVQ N+Q+Y   ++FRYI VGNEV+P  S   ++L AM+NI 
Sbjct: 85  VPSSDLERLASSQTEADKWVQENVQSYRDGVRFRYINVGNEVKP--SVGGFLLQAMQNIE 144

Query: 165 SAIVAANLQDHMNVSTVITTDLLGSSFPPSVGSFRSDAREFIEPIISFLAKNGSPLLANV 224
           +A+  A L+  + VST I TD    + PPS G FR + + F+EP+I FLA   SPLL N+
Sbjct: 145 NAVSGAGLE--VKVSTAIATDTTTDTSPPSQGRFRDEYKSFLEPVIGFLASKQSPLLVNL 204

Query: 225 YPYYIYISNPTSINLEYALFTASRPVIVDGNLEYYNFVKTIIDALYAALEKSGGANVSIV 284
           YPY+ Y+ +  +I+L+YALFTA   V  D    Y N     +D++YAALEKSGG ++ IV
Sbjct: 205 YPYFSYMGDTANIHLDYALFTAQSTVDNDPGYSYQNLFDANLDSVYAALEKSGGGSLEIV 264

Query: 285 ISESGWPSGTGTNPVIENAVAETYYQNLIRFLQSGTPRRPERAIETYLYGMFDENLMSSE 344
           +SE+GWP+       +EN  A+TY  NLI+ +++G+PRRP +AIETY++ MFDEN     
Sbjct: 265 VSETGWPTEGAVGTSVEN--AKTYVNNLIQHVKNGSPRRPGKAIETYIFAMFDENKKEPT 324

Query: 345 TAKHFGLFKLDKTPKYRLKFS 365
             K +GLF  D+  KY + F+
Sbjct: 325 YEKFWGLFHPDRQSKYEVNFN 339

[Close]

BLAST of Spg022595 vs. TAIR 10
Match: AT3G57240.1 (beta-1,3-glucanase 3 )

HSP 1 Score: 291.2 bits (744), Expect = 1.2e-78
Identity = 154/315 (48.89%), Postives = 211/315 (66.98%), Query Frame = 0


Query: 52  IGVCYGSKGDNLPEPREVVELCKSYGIKRMRIYDPNKAMLDALRGSNIEVILGMPNTDIE 111
           IGVCYG  G+NL    EVV L +   I+RMR+YDPN+  L+ALRGSNIE++L +PN D++
Sbjct: 35  IGVCYGRNGNNLRPASEVVALYQQRNIRRMRLYDPNQETLNALRGSNIELVLDVPNPDLQ 94

Query: 112 GIANFSFAAN-WVQRNIQAYVPDIKFRYIAVGNEVRPSDSFASYVLPAMRNIYSAIVAAN 171
            +A+    A+ WV+ N++ Y  ++ FRYI+VGNEV+PSD  AS+VLPAM+NI  A+ +  
Sbjct: 95  RLASSQAEADTWVRNNVRNYA-NVTFRYISVGNEVQPSDQAASFVLPAMQNIERAVSSLG 154

Query: 172 LQDHMNVSTVITTDLLGSSFPPSVGSFRSDAREFIEPIISFLAKNGSPLLANVYPYYIYI 231
           ++    VST I T  + S FPPS G+F  + R FI P+ISFL+   SPLL N YPY+ Y 
Sbjct: 155 IK----VSTAIDTRGI-SGFPPSSGTFTPEFRSFIAPVISFLSSKQSPLLVNNYPYFSYT 214

Query: 232 SNPTSINLEYALFTASRPVIVDGNLEYYNFVKTIIDALYAALEKSGGANVSIVISESGWP 291
            N   I L+Y LFTA   V+ DG  +Y N    I+D +YA+LEK+GG ++ IV+SESGWP
Sbjct: 215 GNMRDIRLDYTLFTAPSTVVNDGQNQYRNLFHAILDTVYASLEKAGGGSLEIVVSESGWP 274

Query: 292 SGTGTNPVIENAVAETYYQNLIRFLQSGTPRRPERAIETYLYGMFDEN-LMSSETAKHFG 351
           +  G    ++N  A TY  NLI+ +++G+PRRP RA ETY++ MFDEN     ET K +G
Sbjct: 275 TAGGAATGVDN--ARTYVNNLIQTVKNGSPRRPGRATETYIFAMFDENSKQGPETEKFWG 334

Query: 352 LFKLDKTPKYRLKFS 365
           LF  +  PKY + F+
Sbjct: 335 LFLPNLQPKYVVNFN 341

[Close]

BLAST of Spg022595 vs. TAIR 10
Match: AT5G20330.1 (beta-1,3-glucanase 4 )

HSP 1 Score: 245.0 bits (624), Expect = 9.5e-65
Identity = 130/322 (40.37%), Postives = 195/322 (60.56%), Query Frame = 0


Query: 45  FVATAEAIGVCYGSKGDNLPEPREVVELCKSYGIKRMRIYDPNKAMLDALRG-SNIEVIL 104
           FV  A +IG+ YG  GDNLP P  V+ L KS GI R+RI+DPN  +L+ALRG  +IEV +
Sbjct: 27  FVTAANSIGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFDPNTEVLNALRGHRDIEVTV 86

Query: 105 GMPNTDIEGIANFSFAA-NWVQRNIQAYVPDIKFRYIAVGNEVRPSDSFASYVLPAMRNI 164
           G+ + D+  +A    A   W   NI++Y+ D+   +I VGNEV P       VLP M+++
Sbjct: 87  GVKDQDLAALAASEEAVKGWFAANIESYLADVNITFITVGNEVIPG-PIGPQVLPVMQSL 146

Query: 165 YSAIVAANLQDHMNVSTVITTDLLGSSFPPSVGSFRSDAREFIEPIISFLAKNGSPLLAN 224
            + + + NL   +++STV+    L  S+PPS G F S ARE + P++  L++  +P+L N
Sbjct: 147 TNLVKSRNLP--ISISTVVAMSNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTSTPILVN 206

Query: 225 VYPYYIYISNPTSINLEYALFTASRPVIVDGNLEYYNFVKTIIDALYAALEKSGGANVSI 284
           +YPY+ Y S+P +I L+YA F     V+ DG+L Y N    I DA   A+EK G  N+ +
Sbjct: 207 IYPYFAYASDPANIRLDYASFNTKSIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKNLPM 266

Query: 285 VISESGWPSGTGTNPVIENAVAETYYQNLIRFLQS--GTPRRPERAIETYLYGMFDENLM 344
           V+SE+GWPS  G       A+A TY +N ++ + S  GTP+RP +++  +L+  F+EN  
Sbjct: 267 VVSETGWPS-AGNGNFTTPAIASTYNRNFVKHIASGKGTPKRPNKSMNGFLFATFNENQK 326

Query: 345 SSETAKHFGLFK-LDKTPKYRL 362
            + T ++FGL+   D  P Y+L
Sbjct: 327 PAGTEQNFGLYNPSDMKPIYKL 344

The following BLAST results are available for this feature:

BLAST of Spg022595 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022154376.1	5.1e-145	71.78	glucan endo-1,3-beta-glucosidase-like [Momordica charantia]	[more]
XP_022154361.1	8.7e-145	71.98	glucan endo-1,3-beta-glucosidase-like isoform X3 [Momordica charantia]	[more]
XP_022154360.1	2.5e-128	72.73	glucan endo-1,3-beta-glucosidase-like isoform X2 [Momordica charantia]	[more]
XP_022154359.1	2.5e-128	66.48	glucan endo-1,3-beta-glucosidase-like isoform X1 [Momordica charantia]	[more]
XP_022154372.1	1.1e-126	64.75	glucan endo-1,3-beta-glucosidase-like [Momordica charantia]	[more]

BLAST of Spg022595 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P07979	1.2e-96	54.66	Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3	[more]
A7PQW3	2.0e-96	56.39	Glucan endo-1,3-beta-glucosidase OS=Vitis vinifera OX=29760 GN=VIT_06s0061g00120...	[more]
O22317	5.6e-94	53.48	Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2	[more]
P52407	1.6e-93	49.72	Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform OS=Hevea brasiliensis O...	[more]
P52408	7.5e-91	52.83	Glucan endo-1,3-beta-glucosidase, basic isoform OS=Prunus persica OX=3760 GN=GNS...	[more]

BLAST of Spg022595 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DNI9	2.5e-145	71.78	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111021658 ...	[more]
A0A6J1DNH1	4.2e-145	71.98	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111021643 ...	[more]
A0A6J1DLU4	1.2e-128	66.48	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111021643 ...	[more]
A0A6J1DJE2	1.2e-128	72.73	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111021643 ...	[more]
A0A6J1DLI3	5.2e-127	64.75	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111021653 ...	[more]

BLAST of Spg022595 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G16260.1	6.5e-90	49.53	Glycosyl hydrolase superfamily protein	[more]
AT3G57270.1	1.9e-81	47.34	beta-1,3-glucanase 1	[more]
AT3G57260.1	1.0e-79	47.35	beta-1,3-glucanase 2	[more]
AT3G57240.1	1.2e-78	48.89	beta-1,3-glucanase 3	[more]
AT5G20330.1	9.5e-65	40.37	beta-1,3-glucanase 4	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 50..364 e-value: 5.9E-120 score: 402.0
None	No IPR available	PANTHER	PTHR32227:SF292	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE-LIKE	coord: 44..361
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 52..363 e-value: 2.1E-104 score: 349.5
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 44..361
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 52..361

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg022595.1	Spg022595.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds