Spg022565 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg022565 vs. NCBI nr
Match: XP_038879674.1 (protochlorophyllide-dependent translocon component 52, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 941.4 bits (2432), Expect = 3.4e-270
Identity = 455/548 (83.03%), Postives = 482/548 (87.96%), Query Frame = 0

Query: 1   MDVLKFSSAPLHFTCKLNKSQFPKPCMQIIHLSSSSSSSINRHSRRLKLCASTSSAISAE 60
           MDVLK SS+P HFT  LNK QFP  C+QI   SSSSSSSIN+HSRR KLCA TSSAIS E
Sbjct: 1   MDVLKLSSSPFHFTAILNKPQFPNQCLQIPLFSSSSSSSINKHSRRFKLCAITSSAISTE 60

Query: 61  ESANSFEVEAESKRREERFDWYAQWYPIMPICDLDQRVPHGKTVMGIDVVVWWDKNENAW 120
           ESAN FEVEAES+RREERFDWYAQWYPIMPICDLD+RVPHGKTVMGIDVVVWWDKNE+AW
Sbjct: 61  ESANPFEVEAESERREERFDWYAQWYPIMPICDLDKRVPHGKTVMGIDVVVWWDKNESAW 120

Query: 121 KVFEDLCPHRLAPLSEGRIDQWGRLQCVYHGWCFNGSGDCKFIPQAPPDGPPVHTSKRAC 180
           KVF+DLCPHRLAPLSEGRIDQWGRLQCVYHGWCFNGSGDCKFIPQAPPDGPPVHTSKRAC
Sbjct: 121 KVFDDLCPHRLAPLSEGRIDQWGRLQCVYHGWCFNGSGDCKFIPQAPPDGPPVHTSKRAC 180

Query: 181 VAVYPSIVQNGMLWFWPNSDPQYKDILVKKKPPYIPELDDPSYTKSICSRDLPYGVTEVD 240
           VAVYPSIVQN MLWFWPNSDPQYKDILVKKKPPYIPELDDPS+TK I +RDLPYG   + 
Sbjct: 181 VAVYPSIVQNEMLWFWPNSDPQYKDILVKKKPPYIPELDDPSFTKLISNRDLPYGYEVLT 240

Query: 241 LIVKDRKELECVEKTSMFTQLLCLFALSEKADREGGKPIEMVVRDLDVYGFITDQEQGNG 300
             + D   +       M  Q        EKADREGGKPI++VVRDLDV GFIT+QE G  
Sbjct: 241 ENLMDPAHVPYAHYGIMGRQQP---KNREKADREGGKPIDIVVRDLDVNGFITNQELGTS 300

Query: 301 QFFAPCVFYTSFSPRSKERNSKHQGNGTLLSTENNTVARNTSQRKTMLIFMCIPVSPGKS 360
           QFFAPCVFYTSFS  SK+R+S  Q NG LLS+ENNTVA NTSQRK MLIFMCIPV PGKS
Sbjct: 301 QFFAPCVFYTSFSLPSKKRDSNRQDNGALLSSENNTVAPNTSQRKAMLIFMCIPVGPGKS 360

Query: 361 RVIFIPLRNFAVWIDWFIPRWMFHVGQNLVFDSDLYLLHVEERKILDVGPLNWQKACFVP 420
           R+IF+ LRNFAVWID  IPRW+FHVGQNL+ DSDLYLLHVEERKILDVGP NWQKACFVP
Sbjct: 361 RLIFVSLRNFAVWIDRIIPRWVFHVGQNLILDSDLYLLHVEERKILDVGPSNWQKACFVP 420

Query: 421 TKSDAKVVAFRRWLNKYSDGHVDWRGKFSGILPPSPPREQLMDRYWSHVVNCSSCSVAYK 480
           TKSDAKVVAFRRWLNKYSDG VDWRGKFSGILPPSPP+EQLMDRYWSHVV+CSSC VAYK
Sbjct: 421 TKSDAKVVAFRRWLNKYSDGQVDWRGKFSGILPPSPPKEQLMDRYWSHVVSCSSCRVAYK 480

Query: 481 GLNALEVVLQVISIASLGVFAVTKQSLVSGAARVGMVAMAVLCFAASRWLSHFIYKNFHF 540
           GLNALEVVLQVISIASLG+FA TKQSLVSGAARV MVAMA+LCFAASRWLSH+IYKNFHF
Sbjct: 481 GLNALEVVLQVISIASLGIFAATKQSLVSGAARVLMVAMAILCFAASRWLSHYIYKNFHF 540

Query: 541 HDYNHALR 549
           HDYNHALR
Sbjct: 541 HDYNHALR 545

BLAST of Spg022565 vs. NCBI nr
Match: XP_038879955.1 (protochlorophyllide-dependent translocon component 52, chloroplastic-like isoform X1 [Benincasa hispida])

HSP 1 Score: 911.4 bits (2354), Expect = 3.8e-261
Identity = 432/548 (78.83%), Postives = 478/548 (87.23%), Query Frame = 0

Query: 1   MDVLKFSSAPLHFTCKLNKSQFPKPCMQIIHLSSSSSSSINRHSRRLKLCASTSSAISAE 60
           M+VLK SSAPLHFT K+NKSQFPK C+QI   SSS SSS+NR +RR KLCAS S+ IS E
Sbjct: 1   MNVLKISSAPLHFTTKVNKSQFPKQCLQIPLFSSSFSSSVNRRNRRFKLCASKSTTISTE 60

Query: 61  ESANSFEVEAESKRREERFDWYAQWYPIMPICDLDQRVPHGKTVMGIDVVVWWDKNENAW 120
           ESAN FEVEAES++REERFDWYAQWYPI+PICDLD++VPHGKTVMGID+VVWWDKNE+AW
Sbjct: 61  ESANPFEVEAESEQREERFDWYAQWYPILPICDLDKKVPHGKTVMGIDLVVWWDKNESAW 120

Query: 121 KVFEDLCPHRLAPLSEGRIDQWGRLQCVYHGWCFNGSGDCKFIPQAPPDGPPVHTSKRAC 180
           KVF+DLCPHRLAPLSEGRID+WGRLQCVYHGWCFNGSGDCKFIPQAPPDGPPVHTSKRAC
Sbjct: 121 KVFDDLCPHRLAPLSEGRIDRWGRLQCVYHGWCFNGSGDCKFIPQAPPDGPPVHTSKRAC 180

Query: 181 VAVYPSIVQNGMLWFWPNSDPQYKDILVKKKPPYIPELDDPSYTKSICSRDLPYGVTEVD 240
           V+VYPSIVQN MLWFWPNSDPQYKDI+V KKPPYIPELDDPSYTK I +RDLPYG   + 
Sbjct: 181 VSVYPSIVQNDMLWFWPNSDPQYKDIMVTKKPPYIPELDDPSYTKLIINRDLPYGYEVLI 240

Query: 241 LIVKDRKELECVEKTSMFTQLLCLFALSEKADREGGKPIEMVVRDLDVYGFITDQEQGNG 300
             + D   +       +  Q       + KADREGGKPIE+VVRD DV+GFI++QE GN 
Sbjct: 241 ENLMDPAHVPYAHYGIIGRQQ----TKNRKADREGGKPIEIVVRDFDVHGFISNQEVGNS 300

Query: 301 QFFAPCVFYTSFSPRSKERNSKHQGNGTLLSTENNTVARNTSQRKTMLIFMCIPVSPGKS 360
           +F APCV YTSF+  SKERNS+HQ NGTLL  ENN V  +TSQRKTM++FMCIP+SPGKS
Sbjct: 301 KFVAPCVLYTSFNLPSKERNSRHQDNGTLLLAENNPVTPSTSQRKTMVVFMCIPISPGKS 360

Query: 361 RVIFIPLRNFAVWIDWFIPRWMFHVGQNLVFDSDLYLLHVEERKILDVGPLNWQKACFVP 420
           RVIFIPLRNFA+W++  IPRWMFH+GQNLV DSD YLLHVEERKILDVGP NWQKACF+P
Sbjct: 361 RVIFIPLRNFAIWVNRIIPRWMFHLGQNLVLDSDSYLLHVEERKILDVGPSNWQKACFLP 420

Query: 421 TKSDAKVVAFRRWLNKYSDGHVDWRGKFSGILPPSPPREQLMDRYWSHVVNCSSCSVAYK 480
           TKSDAKVVA RRWLNKYSDGHVDWRGKFSGILPPSPP+++LMDRYWSHVVNCSSC VAYK
Sbjct: 421 TKSDAKVVALRRWLNKYSDGHVDWRGKFSGILPPSPPKKKLMDRYWSHVVNCSSCKVAYK 480

Query: 481 GLNALEVVLQVISIASLGVFAVTKQSLVSGAARVGMVAMAVLCFAASRWLSHFIYKNFHF 540
            LNALEVVLQV+SIASLG+ A TKQSL SGAARV MV+MA+L FAASRWLSHFIYKNFHF
Sbjct: 481 VLNALEVVLQVMSIASLGILAATKQSLASGAARVLMVSMAILFFAASRWLSHFIYKNFHF 540

Query: 541 HDYNHALR 549
           HDYNHALR
Sbjct: 541 HDYNHALR 544

BLAST of Spg022565 vs. NCBI nr
Match: XP_022933573.1 (protochlorophyllide-dependent translocon component 52, chloroplastic [Cucurbita moschata])

HSP 1 Score: 910.6 bits (2352), Expect = 6.5e-261
Identity = 444/551 (80.58%), Postives = 474/551 (86.03%), Query Frame = 0

Query: 1   MDVLKFSSAPLHFTCKLNKSQFPKPCMQIIHLSSSSSSSINRHSRRLKLCASTSSAISAE 60
           MDVLKFSS PLHFT KL KS+F K C+Q+  L S  SSSI+RHSRR KL AS SS IS E
Sbjct: 1   MDVLKFSSVPLHFTAKLKKSRFLKQCLQVNLLPSLPSSSISRHSRRFKLRASVSSTISTE 60

Query: 61  --ESANSFEVEAESKRRE-ERFDWYAQWYPIMPICDLDQRVPHGKTVMGIDVVVWWDKNE 120
             E A+ FEVEAE++RRE ERFDWYAQWYPIMPICDLD+RVPHGKTVMGIDVVVWWDKNE
Sbjct: 61  EFEFASPFEVEAENERREDERFDWYAQWYPIMPICDLDKRVPHGKTVMGIDVVVWWDKNE 120

Query: 121 NAWKVFEDLCPHRLAPLSEGRIDQWGRLQCVYHGWCFNGSGDCKFIPQAPPDGPPVHTSK 180
           +AWKVFEDLCPHRLAPLSEGRIDQWGRLQCVYHGWCFNGSGDCKFIPQAPPDGPPVHTSK
Sbjct: 121 SAWKVFEDLCPHRLAPLSEGRIDQWGRLQCVYHGWCFNGSGDCKFIPQAPPDGPPVHTSK 180

Query: 181 RACVAVYPSIVQNGMLWFWPNSDPQYKDILVKKKPPYIPELDDPSYTKSICSRDLPYGVT 240
           RACVAVYPSIVQN +LWFWPNSDPQYKDILVKKKPPYIPELDDPSYTK I +RDLPYG  
Sbjct: 181 RACVAVYPSIVQNDILWFWPNSDPQYKDILVKKKPPYIPELDDPSYTKLISNRDLPYGYE 240

Query: 241 EVDLIVKDRKELECVEKTSMFTQLLCLFALSEKADREGGKPIEMVVRDLDVYGFITDQEQ 300
            +   + D   +       M  Q        EKADREGGKPIE+ ++DLD YGFI++QE 
Sbjct: 241 VLTENLMDPAHVPYAHYGIMGRQQP---NNREKADREGGKPIEIFIKDLDAYGFISNQEL 300

Query: 301 GNGQFFAPCVFYTSFSPRSKERNSKHQGNGTLLSTENNTVARNTSQRKTMLIFMCIPVSP 360
           GN QFFAPCVFYTSFS RSK+R  KHQ NG LLS ENN  A NTSQRK ML+FMCIPVSP
Sbjct: 301 GNSQFFAPCVFYTSFSLRSKKRTPKHQDNGALLSAENNAEAPNTSQRKAMLVFMCIPVSP 360

Query: 361 GKSRVIFIPLRNFAVWIDWFIPRWMFHVGQNLVFDSDLYLLHVEERKILDVGPLNWQKAC 420
           G+SR+IF+ LRNFA+ ID  IPRW+FHVGQNL+ DSDLYLLHVEERKILDVGP NWQKAC
Sbjct: 361 GRSRLIFVSLRNFAIGIDRIIPRWVFHVGQNLILDSDLYLLHVEERKILDVGPSNWQKAC 420

Query: 421 FVPTKSDAKVVAFRRWLNKYSDGHVDWRGKFSGILPPSPPREQLMDRYWSHVVNCSSCSV 480
           FVPTKSDAKVVAFRRWLNKYSDGHVDWRGKFSGILPPSPP+EQLMDRYWSHVVNCSSC+ 
Sbjct: 421 FVPTKSDAKVVAFRRWLNKYSDGHVDWRGKFSGILPPSPPKEQLMDRYWSHVVNCSSCNG 480

Query: 481 AYKGLNALEVVLQVISIASLGVFAVTKQSLVSGAARVGMVAMAVLCFAASRWLSHFIYKN 540
           AYKGLNALEVVLQVISIA LGVFA T QSLVS  AR+ MVAMAVLCFAASRWLSHFIYKN
Sbjct: 481 AYKGLNALEVVLQVISIALLGVFAATNQSLVSRTARIVMVAMAVLCFAASRWLSHFIYKN 540

Query: 541 FHFHDYNHALR 549
           FHFHDYNHALR
Sbjct: 541 FHFHDYNHALR 548

BLAST of Spg022565 vs. NCBI nr
Match: XP_023531366.1 (protochlorophyllide-dependent translocon component 52, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 909.1 bits (2348), Expect = 1.9e-260
Identity = 442/551 (80.22%), Postives = 474/551 (86.03%), Query Frame = 0

Query: 1   MDVLKFSSAPLHFTCKLNKSQFPKPCMQIIHLSSSSSSSINRHSRRLKLCASTSSAISAE 60
           MDVLKFSS PLHFT KLNKS+F K C+Q+  L S  SSSI+R+SRR KL AS SS IS E
Sbjct: 1   MDVLKFSSVPLHFTAKLNKSRFLKQCLQVNLLPSLPSSSISRYSRRFKLRASVSSTISTE 60

Query: 61  --ESANSFEVEAESKRR-EERFDWYAQWYPIMPICDLDQRVPHGKTVMGIDVVVWWDKNE 120
             E A+ FEVEAES+RR +ERFDWYAQWYPIMPICDLD+RVPHGKTVMGIDVVVWWDKNE
Sbjct: 61  EFEFASPFEVEAESERRVDERFDWYAQWYPIMPICDLDKRVPHGKTVMGIDVVVWWDKNE 120

Query: 121 NAWKVFEDLCPHRLAPLSEGRIDQWGRLQCVYHGWCFNGSGDCKFIPQAPPDGPPVHTSK 180
           +AWKVFEDLCPHRLAPLSEGRIDQWGRLQCVYHGWCFNGSGDCKFIPQAPPDGPPVHTSK
Sbjct: 121 SAWKVFEDLCPHRLAPLSEGRIDQWGRLQCVYHGWCFNGSGDCKFIPQAPPDGPPVHTSK 180

Query: 181 RACVAVYPSIVQNGMLWFWPNSDPQYKDILVKKKPPYIPELDDPSYTKSICSRDLPYGVT 240
           RACVAVYPSIVQN +LWFWPNSDPQYKDILVKK PPYIPELDDPSYTK I +RDLPYG  
Sbjct: 181 RACVAVYPSIVQNDILWFWPNSDPQYKDILVKKNPPYIPELDDPSYTKLISNRDLPYGYE 240

Query: 241 EVDLIVKDRKELECVEKTSMFTQLLCLFALSEKADREGGKPIEMVVRDLDVYGFITDQEQ 300
            +   + D   +       M  Q        EKADREGGKPIE+ ++DLD YGFI++QE 
Sbjct: 241 VLTENLMDPAHVPYAHYGIMGRQQP---NNREKADREGGKPIEIFIKDLDAYGFISNQEL 300

Query: 301 GNGQFFAPCVFYTSFSPRSKERNSKHQGNGTLLSTENNTVARNTSQRKTMLIFMCIPVSP 360
           GN QFFAPCVFYTSFS RSK+R  KHQ NG LLS ENN  A NTSQRK ML+FMCIPVSP
Sbjct: 301 GNSQFFAPCVFYTSFSLRSKKRTPKHQDNGALLSAENNAEAPNTSQRKAMLVFMCIPVSP 360

Query: 361 GKSRVIFIPLRNFAVWIDWFIPRWMFHVGQNLVFDSDLYLLHVEERKILDVGPLNWQKAC 420
           G+SR+IF+ LRNFA+ ID  IPRW+FHVGQNL+ DSDLYLLHVEERKILDVGP NWQKAC
Sbjct: 361 GRSRLIFVSLRNFAIGIDRIIPRWVFHVGQNLILDSDLYLLHVEERKILDVGPSNWQKAC 420

Query: 421 FVPTKSDAKVVAFRRWLNKYSDGHVDWRGKFSGILPPSPPREQLMDRYWSHVVNCSSCSV 480
           FVPTKSDAKVVAFRRWLNKYSDGHVDWRGKFSGILPPSPP+EQLMDRYWSHVVNCSSC+ 
Sbjct: 421 FVPTKSDAKVVAFRRWLNKYSDGHVDWRGKFSGILPPSPPKEQLMDRYWSHVVNCSSCNG 480

Query: 481 AYKGLNALEVVLQVISIASLGVFAVTKQSLVSGAARVGMVAMAVLCFAASRWLSHFIYKN 540
           AYKGLN LEVVLQVISIA LGVFA T QSLVSG AR+ MVAMAVLCFAAS+WLSHFIYKN
Sbjct: 481 AYKGLNTLEVVLQVISIALLGVFAATNQSLVSGTARIVMVAMAVLCFAASKWLSHFIYKN 540

Query: 541 FHFHDYNHALR 549
           FHFHDYNHALR
Sbjct: 541 FHFHDYNHALR 548

BLAST of Spg022565 vs. NCBI nr
Match: XP_008451029.1 (PREDICTED: protochlorophyllide-dependent translocon component 52, chloroplastic-like [Cucumis melo])

HSP 1 Score: 902.5 bits (2331), Expect = 1.8e-258
Identity = 433/548 (79.01%), Postives = 474/548 (86.50%), Query Frame = 0

Query: 1   MDVLKFSSAPLHFTCKLNKSQFPKPCMQIIHLSSSSSSSINRHSRRLKLCASTSSAISAE 60
           MD+LK SS+PLHFT K +K QFP  C++I    SSSSSSINR + R KLCA TSSAIS +
Sbjct: 1   MDLLKLSSSPLHFTPKFDKPQFPNQCLRIPLFPSSSSSSINRRNPRFKLCAITSSAISTQ 60

Query: 61  ESANSFEVEAESKRREERFDWYAQWYPIMPICDLDQRVPHGKTVMGIDVVVWWDKNENAW 120
           ESAN F+VEAES+  EERFDWYAQWYPIMPICDLD+RVPHGKTVMGIDVVVWWDKNE+AW
Sbjct: 61  ESANPFQVEAESESGEERFDWYAQWYPIMPICDLDKRVPHGKTVMGIDVVVWWDKNESAW 120

Query: 121 KVFEDLCPHRLAPLSEGRIDQWGRLQCVYHGWCFNGSGDCKFIPQAPPDGPPVHTSKRAC 180
           KVF+DLCPHRLAPLSEGRIDQWGRLQCVYHGWCFNGSGDCKFIPQAPPDGPPVHTSKRAC
Sbjct: 121 KVFDDLCPHRLAPLSEGRIDQWGRLQCVYHGWCFNGSGDCKFIPQAPPDGPPVHTSKRAC 180

Query: 181 VAVYPSIVQNGMLWFWPNSDPQYKDILVKKKPPYIPELDDPSYTKSICSRDLPYGVTEVD 240
           VAVYPSIVQN +LWFWPNSDPQYKDI+VKKKPPYIPELDDPS+TK I +RDLPYG   + 
Sbjct: 181 VAVYPSIVQNDVLWFWPNSDPQYKDIMVKKKPPYIPELDDPSFTKLISNRDLPYGYEILT 240

Query: 241 LIVKDRKELECVEKTSMFTQLLCLFALSEKADREGGKPIEMVVRDLDVYGFITDQEQGNG 300
             + D   +       M  Q        EKADREGGKPI++VVRDL++ GFI++Q+ G+ 
Sbjct: 241 ENLMDPAHVPYAHYGIMGRQQP---KNREKADREGGKPIDIVVRDLNINGFISNQDLGSS 300

Query: 301 QFFAPCVFYTSFSPRSKERNSKHQGNGTLLSTENNTVARNTSQRKTMLIFMCIPVSPGKS 360
           QFFAPCVF+TSFS  SK+ NS  Q +G LLS ENN VA NTSQRK MLIFMCIPV PGKS
Sbjct: 301 QFFAPCVFFTSFSLPSKKTNSNRQDSGALLSGENNAVAPNTSQRKAMLIFMCIPVGPGKS 360

Query: 361 RVIFIPLRNFAVWIDWFIPRWMFHVGQNLVFDSDLYLLHVEERKILDVGPLNWQKACFVP 420
           R+IF+ LRNFAV ID  IPRW+FHVGQNL+ DSDLYLLH+EERKILDVGP NWQKACFVP
Sbjct: 361 RLIFVSLRNFAVSIDRIIPRWIFHVGQNLILDSDLYLLHIEERKILDVGPSNWQKACFVP 420

Query: 421 TKSDAKVVAFRRWLNKYSDGHVDWRGKFSGILPPSPPREQLMDRYWSHVVNCSSCSVAYK 480
           TKSDAKVVAFRRWLN+YSDG VDWRGKF+GI PPSPP+EQLMDRYWSHVVNCSSC VAYK
Sbjct: 421 TKSDAKVVAFRRWLNRYSDGQVDWRGKFNGIFPPSPPKEQLMDRYWSHVVNCSSCKVAYK 480

Query: 481 GLNALEVVLQVISIASLGVFAVTKQSLVSGAARVGMVAMAVLCFAASRWLSHFIYKNFHF 540
           GLNALEVVLQVISIASLG+FA TKQSLVSGAARV MVAMA+LCFA+SRWLSHFIYKNFHF
Sbjct: 481 GLNALEVVLQVISIASLGIFAATKQSLVSGAARVLMVAMAILCFASSRWLSHFIYKNFHF 540

Query: 541 HDYNHALR 549
           HDYNHALR
Sbjct: 541 HDYNHALR 545

BLAST of Spg022565 vs. ExPASy Swiss-Prot
Match: Q8W496 (Protochlorophyllide-dependent translocon component 52, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PTC52 PE=2 SV=1)

HSP 1 Score: 644.0 bits (1660), Expect = 1.5e-183
Identity = 315/541 (58.23%), Postives = 390/541 (72.09%), Query Frame = 0

Query: 32  LSSSSSSSINRHSRRLKLCASTSSAISAEESANS-FEVEAESKRREERFDWYAQWYPIMP 91
           L  S +S I R S R     S+  + SA  S NS  E EA  +   ++FDWYA WYP+MP
Sbjct: 31  LPISPNSLITRKSSRFTTAVSSPPSSSAATSTNSPPEPEALFEPGSDKFDWYANWYPVMP 90

Query: 92  ICDLDQRVPHGKTVMGIDVVVWWDKNENAWKVFEDLCPHRLAPLSEGRIDQWGRLQCVYH 151
           ICDLD++VPHGK VMGID+VVWWD+NE  WKV +D CPHRLAPLS+GRIDQWGRLQCVYH
Sbjct: 91  ICDLDKKVPHGKKVMGIDLVVWWDRNEKQWKVMDDTCPHRLAPLSDGRIDQWGRLQCVYH 150

Query: 152 GWCFNGSGDCKFIPQAPPDGPPVHTSKRACVAVYPSIVQNGMLWFWPNSDPQYKDILVKK 211
           GWCFNGSGDCK IPQAPPDGPPVHT K+ACVAVYPS VQ+ ++WFWPNSDP+YK+I+   
Sbjct: 151 GWCFNGSGDCKLIPQAPPDGPPVHTFKQACVAVYPSTVQHEIIWFWPNSDPKYKNIIETN 210

Query: 212 KPPYIPELDDPSYTKSICSRDLPYGVTEVDLIVKD---------------------RKEL 271
           KPPYIPEL+DPS+TK + +RD+PYG    D++V++                      K +
Sbjct: 211 KPPYIPELEDPSFTKLMGNRDIPYG---YDVLVENLMDPAHVPYAHYGLMRFPKPKGKYI 270

Query: 272 ECVEKT--SMFTQLLCLFALSEKADREGGKPIEMVVRDLDVYGFITDQEQGNGQFFAPCV 331
            C+  +  + FT L  L  L+EK DREGGKP+E+ V+ LD  GF + QE G   F APCV
Sbjct: 271 ICISNSCFNPFTNLQIL--LAEKIDREGGKPLEINVKKLDNKGFFSKQEWGYSNFIAPCV 330

Query: 332 FYTSFSPRSKERNSKHQGNGTLLSTENNTVARNTSQRKTMLIFMCIPVSPGKSRVIFIPL 391
           + +S  P  ++   +H+      S +        S+R+  LIF+CIPVSPG+SR+I+   
Sbjct: 331 YRSSTDPLPEQ---EHEYPAPAASDK-----AALSKRRLSLIFICIPVSPGRSRLIWTFP 390

Query: 392 RNFAVWIDWFIPRWMFHVGQNLVFDSDLYLLHVEERKILDVGPLNWQKACFVPTKSDAKV 451
           RNF V+ID  +PRW+FH+GQN + DSDL+LLHVEERKIL+ GP NWQKACF+PTKSDA V
Sbjct: 391 RNFGVFIDKIVPRWVFHIGQNTILDSDLHLLHVEERKILERGPENWQKACFIPTKSDANV 450

Query: 452 VAFRRWLNKYSDGHVDWRGKFSG-ILPPSPPREQLMDRYWSHVVNCSSCSVAYKGLNALE 511
           V FRRW NKYS+  VDWRGKF   +LPP+PPREQL DRYWSHV NCSSC  A+K LNALE
Sbjct: 451 VTFRRWFNKYSEARVDWRGKFDPFLLPPTPPREQLFDRYWSHVENCSSCKKAHKYLNALE 510

Query: 512 VVLQVISIASLGVFAVTKQSLVSGAARVGMVAMAVLCFAASRWLSHFIYKNFHFHDYNHA 548
           V+LQ+ S+A +GV AV KQ+ +S  AR+ ++  AVL FAAS+WLSHFIYK FH+HDYNHA
Sbjct: 511 VILQIASVAMIGVMAVLKQTTMSNVARIAVLVAAVLSFAASKWLSHFIYKTFHYHDYNHA 558

BLAST of Spg022565 vs. ExPASy Swiss-Prot
Match: Q0DV66 (Pheophorbide a oxygenase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=PAO PE=2 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 2.4e-64
Identity = 172/542 (31.73%), Postives = 248/542 (45.76%), Query Frame = 0

Query: 32  LSSSSSSSINRHSRRLKLCASTSSAISAEESANSFEVEAESKRREERFDWYAQWYPIMPI 91
           LS+S    + R     +L  +   ++  E      E E  +   +E+F W   WYP+  +
Sbjct: 29  LSASGRLRLRRARADTRLRVAAPPSVPGEADQAPGETEPSTSSADEKFVWRDHWYPVSLV 88

Query: 92  CDLDQRVPHGKTVMGIDVVVWWDKNENAWKVFEDLCPHRLAPLSEGRIDQWGRLQCVYHG 151
            DLD  VP    ++  D+V+W D     W   +D CPHRLAPLSEGRID+ G LQC YHG
Sbjct: 89  EDLDPSVPTPFQLLNRDLVIWKDPKSGEWVALDDRCPHRLAPLSEGRIDETGCLQCSYHG 148

Query: 152 WCFNGSGDCKFIPQAPPDGPPVHT--SKRACVAVYPSIVQNGMLWFWPNSDPQYKDILVK 211
           W F+GSG C  IPQA P+GP      S +AC   +P++V  G+L+ WP+ +   K     
Sbjct: 149 WSFDGSGACTRIPQAAPEGPEAKAVRSPKACAIKFPTLVSQGLLFVWPDENGWEK--ATA 208

Query: 212 KKPPYIP-ELDDPSYTKSICSRDLPYGVTEVDLIVKDRKELECVEKTSMFTQLLCLFALS 271
            KPP +P E +DP+++     RDL YG   +   V D   +E                  
Sbjct: 209 TKPPMLPKEFEDPAFSTVTIQRDLYYGYDTLMENVSDPSHIEFAHH-------------K 268

Query: 272 EKADREGGKPIEMVVRDLDVYGFITDQEQGN----GQFFAPCVFYTSFSPRSKERNSKHQ 331
               R+  +P+   +     +G+ +    GN      F APC                  
Sbjct: 269 VTGRRDRARPLPFKMESSGAWGY-SGSNSGNPRISATFVAPCY----------------- 328

Query: 332 GNGTLLSTENNTVARNTSQRKTMLIFMC---IPVSPGKSRVIFIPLRNF--------AVW 391
               L   E +T       +K  +I++C   IP++PGK+R I    RNF        A W
Sbjct: 329 ---ALNKIEIDTKLPIFGDQK-WVIWICSFNIPMAPGKTRSIVCSARNFFQFSMPGKAWW 388

Query: 392 IDWFIPRWMFHVGQNLVFDSDLYLLHVEERKILDV-----GPLNWQ--KACFVPTKSDAK 451
               +PRW  H   NLV+D D+ +L  +E+  L         +N Q  K  F PT++D  
Sbjct: 389 --QLVPRWYEHWTSNLVYDGDMIVLQGQEKIFLSASKESSADINQQYTKITFTPTQADRF 448

Query: 452 VVAFRRWLNKYSDGHVDWRGKFSGILPPSP--PREQLMDRYWSHVVNCSSCSVAYKGLNA 511
           V+AFR WL K+ +   DW G  S  + PS    + +++DRY  H + CSSC  AY     
Sbjct: 449 VLAFRAWLRKFGNSQPDWFGNPSQEVLPSTVLSKREMLDRYEQHTLKCSSCKGAYNAFQT 508

Query: 512 LEVVLQVISIASLGVFAVTKQSLVSGAARVGMVAMAVLCFAASRWLSHFIYKNFHFHDYN 547
           L+ V    ++A    F  T         R+ ++A A L  AA  +  + + KNF F DY 
Sbjct: 509 LQKVFMGATVA----FCATAGIPADVQFRL-LLAAAALVSAAVAYAFYTLQKNFVFVDYV 526

BLAST of Spg022565 vs. ExPASy Swiss-Prot
Match: Q9FYC2 (Pheophorbide a oxygenase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PAO PE=1 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 7.2e-61
Identity = 174/545 (31.93%), Postives = 245/545 (44.95%), Query Frame = 0

Query: 34  SSSSSSINRHSRRLKLCASTSSAISAEESANSFEVEAESKRREERFDWYAQWYPIMPICD 93
           S S S +  +  R+    S  ++ S EE     E   + +     F W   WYP+  + D
Sbjct: 37  SPSRSKLFHNPLRVAAPPSVPTSDSTEEKRIEEEYGGDKEEEGSEFKWRDHWYPVSLVED 96

Query: 94  LDQRVPHGKTVMGIDVVVWWDKNENAWKVFEDLCPHRLAPLSEGRIDQWGRLQCVYHGWC 153
           LD  VP    ++G D+V+W+D+N+  W  F+DLCPHRLAPLSEGR+D+ G LQC YHGW 
Sbjct: 97  LDPNVPTPFQLLGRDLVLWFDRNDQKWAAFDDLCPHRLAPLSEGRLDENGHLQCSYHGWS 156

Query: 154 FNGSGDCKFIPQAPPDGPPVHT--SKRACVAVYPSIVQNGMLWFWPNSDPQYKDILVKKK 213
           F G G C  IPQA   GP      S RAC   +P++V  G+L+ WP  D    D     +
Sbjct: 157 FGGCGSCTRIPQAATSGPEARAVKSPRACAIKFPTMVSQGLLFVWP--DENGWDRANSIE 216

Query: 214 PPYIP-ELDDPSYTKSICSRDLPYGVTEVDLIVKDRKELECVEKTSMFTQLLCLFALSEK 273
           PP +P + D P ++     RDL YG   +   V D   ++                    
Sbjct: 217 PPRLPDDFDKPEFSTVTIQRDLFYGYDTLMENVSDPSHIDFAHH-------------KVT 276

Query: 274 ADREGGKPIEMVVRDLDVYGF---ITDQEQGNGQFFAPCVFYTSFSPRSKERNSKHQGNG 333
             R+  KP+   V     +GF     D  +   +F APC     +S    E ++K    G
Sbjct: 277 GRRDRAKPLPFKVESSGPWGFQGANDDSPRITAKFVAPC-----YSMNKIELDAKLPIVG 336

Query: 334 TLLSTENNTVARNTSQRKTMLIFMC---IPVSPGKSRVIFIPLRNF--------AVWIDW 393
                            +  +I++C   IP++PGK+R I    RNF        A W   
Sbjct: 337 ----------------NQKWVIWICSFNIPMAPGKTRSIVCSARNFFQFSVPGPAWW--Q 396

Query: 394 FIPRWMFHVGQNLVFDSDLYLLHVEERKIL---------DVGPLNWQKACFVPTKSDAKV 453
            +PRW  H   NLV+D D+ +L  +E+  L         DV    + K  F PT++D  V
Sbjct: 397 VVPRWYEHWTSNLVYDGDMIVLQGQEKVFLAKSMESPDYDVNK-QYTKLTFTPTQADRFV 456

Query: 454 VAFRRWLNKYSDGHVDWRGKFSGILP-PSP--PREQLMDRYWSHVVNCSSCSVAYKGLNA 513
           +AFR WL ++     +W G      P PS    + Q++DR+  H   CSSC  AY     
Sbjct: 457 LAFRNWLRRHGKSQPEWFGSTPSNQPLPSTVLTKRQMLDRFDQHTQVCSSCKGAYNSFQI 516

Query: 514 LEVVL---QVISIASLGVFAVTKQSLVSGAARVGMVAMAVLCFAASRWLSHFIYKNFHFH 547
           L+  L    V   A+ GV +  +  LV        +A   L  AAS +  H   KNF F 
Sbjct: 517 LKKFLVGATVFWAATAGVPSDVQIRLV--------LAGLSLISAASAYALHEQEKNFVFR 534

BLAST of Spg022565 vs. ExPASy Swiss-Prot
Match: Q9SK50 (Protein TIC 55, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC55 PE=1 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 1.5e-34
Identity = 136/534 (25.47%), Postives = 221/534 (41.39%), Query Frame = 0

Query: 45  RRLKLCASTSSAISAE-ESANSFEVEAESKRREE--RFDWYAQWYPIMPICDLDQRVPHG 104
           R L+  A   +A+S + E      +  E ++R E   +DW  +WYP+    ++ +  P G
Sbjct: 45  RLLRRSAVAGTAVSDQTEGGGDVLLNPEEEKRVEVADYDWTEEWYPLYLTKNVPEDAPLG 104

Query: 105 KTVMGIDVVVWWDKNENAWKVFEDLCPHRLAPLSEGRIDQWGRLQCVYHGWCFNGSGDCK 164
            TV    +V++ D  E   + +ED CPHRLA LSEG++   GRL+C+YHGW F G G C 
Sbjct: 105 LTVYDRQIVLYKD-GEGTLRCYEDRCPHRLAKLSEGQLID-GRLECLYHGWQFEGEGKCV 164

Query: 165 FIPQAPPDGPPVHTSKRACVAVYPSIVQNGMLWFWPNSDPQYKDILVKKKPPYIPELDDP 224
            IPQ P         K ACV  Y      G++W W ++    K     +K P+      P
Sbjct: 165 KIPQLPAS---AKIPKAACVKTYEVKDSQGVVWVWMST----KTPPNPEKLPWFENFARP 224

Query: 225 SYTKSICSRDLPYGVTEVDLIVKDRKELECVEKTSMFTQLLCLFALSEKADREGGKPIEM 284
            +     + +LPY  + +   + D   +      + FT           A RE  +P+  
Sbjct: 225 GFFDISTTHELPYDHSILLENLMDPAHVPISHDRTDFT-----------AKREDAQPLVF 284

Query: 285 VVRDLDVYGFI----TDQEQGNG----QFFAPCVFYTSFSPRSKERNSKHQGNGTLLSTE 344
            V +    GF      ++E G G    +F APCV          + N + +G   + +  
Sbjct: 285 EVTERSNRGFAGTWGREKEGGKGSNLLRFDAPCVL---------QNNREFEGKDGVKNYF 344

Query: 345 NNTVARNTSQRKTMLIFMCIPVSPGKSRVIF---IPLRNFAVWIDWFIPRWMFHVGQNLV 404
           +              +F+C P   GKS +I    +  R+  V +   +P+W +H     V
Sbjct: 345 SG-------------LFLCRPTGQGKSMLIVRFGVTKRSPLVSV---LPQWFWHQNACKV 404

Query: 405 FDSDLYLLHVEERKILDVGPLNWQKACFVPTK--------SDAKVVAFRRWLNKYSDGHV 464
           F+ D+  L  +   ++       +K   VPTK        SD  V  +R+W++K   G  
Sbjct: 405 FEQDMGFLSSQNEVLMK------EK---VPTKDLYLNLKSSDTWVAEYRKWMDKVGHGMP 464

Query: 465 DWRGKFSGILPPSPP---------------------------REQLMDRYWSHVVNCSSC 524
              G  +  LP  PP                              L +RY+ H+++C SC
Sbjct: 465 YHFGHRTISLPKVPPVVEHAPAGLIAALSASYPAKGGIGTMHAPNLANRYFRHIIHCRSC 518

Query: 525 SVAYKGLNALEVVLQVISIA--SLGVFAVTKQSLVSGAARVGMVAMAVLCFAAS 528
           S   K     + +L   ++A  +L +  V++Q       +  ++  A LC AA+
Sbjct: 525 SNVIKSFELWKNILSATAVALTALAILVVSRQ------WKAVLLGSAALCSAAA 518

BLAST of Spg022565 vs. ExPASy Swiss-Prot
Match: O49931 (Protein TIC 55, chloroplastic OS=Pisum sativum OX=3888 GN=TIC55 PE=1 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 3.7e-33
Identity = 136/499 (27.25%), Postives = 203/499 (40.68%), Query Frame = 0

Query: 65  SFEVEAESKRREERFDWYAQWYPIMPICDLDQRVPHGKTVMGIDVVVWWDKNENAWKVFE 124
           S E E + +R    +DW  +WYP+    ++    P G  V   ++V++ D N+  ++ +E
Sbjct: 83  SSEQERKGEREVADYDWTEEWYPLYLTKNVPHDAPLGLKVYDKNIVLFRDGNDQ-FQCYE 142

Query: 125 DLCPHRLAPLSEGRIDQWGRLQCVYHGWCFNGSGDCKFIPQAPPDGPPVHTSKRACVAVY 184
           D CPHRLA LSEG++   GRL+C+YHGW F G G C  IPQ P D       K ACV  Y
Sbjct: 143 DRCPHRLAKLSEGQLID-GRLECLYHGWQFEGEGKCVKIPQLPAD---AKIPKSACVKTY 202

Query: 185 PSIVQNGMLWFWPNSDPQYKDILVKKKPPYIPELDDPSYTKSICSRDLPYGVTEVDLIVK 244
                 G+LW W +     K      K P+      P +     + +LPY  + +   + 
Sbjct: 203 EVRDSQGVLWVWMSR----KTPPNVSKIPWFENFARPGFQDISTTHELPYDHSILLENLM 262

Query: 245 DRKELECVEKTSMFTQLLCLFALSEKADREGGKPIEMVVRDL-DVYGFITDQEQGNG-QF 304
           D   +      + ++      A  E A   G +  E   R     +G   D  + N  +F
Sbjct: 263 DPAHVPISHDRTDWS------AKREDAQALGFEVTERTDRGFAGWWGREKDGSKPNFLRF 322

Query: 305 FAPCVFYTSFSPRSKERNSKHQGNGTLLSTENNTVARNTSQRKTMLIFMCIPVSPGKSRV 364
            APCV                      L      V +N        +F+C P   GKS +
Sbjct: 323 EAPCV----------------------LQNNREIVDKNGEINHFSGLFLCRPTGQGKSML 382

Query: 365 IFIPLRNFAVWIDWFIPRWMFHVGQNLVFDSDLYLLHVEERKILDVGPLNWQKACFVPTK 424
           I          +    P W FH   + VF+ D+  L  +   +L       +K   VPTK
Sbjct: 383 IVRFGATKRSPLIKLFPEWYFHQNASKVFEQDMGFLSSQNEILLK------EK---VPTK 442

Query: 425 --------SDAKVVAFRRWLNK------YSDGHVDW-------------RGKFSGILPPS 484
                   SD  V  +R+W++K      Y  GH                 G  +G+   S
Sbjct: 443 ELYLNLKSSDTWVAEYRKWMDKVGHGMPYHFGHSTISLPEEPAVVEHAPAGLVAGLSASS 502

Query: 485 PPR--------EQLMDRYWSHVVNCSSCSVAYKGLNALEVVLQ--VISIASLGVFAVTKQ 524
           P +          L +RY+ HV++C  CS A K     + VL   V+++A+L +      
Sbjct: 503 PAKGGIGTMHAPNLANRYFRHVIHCKGCSSAIKAFQIWKNVLSGVVVALAALAI------ 528

BLAST of Spg022565 vs. ExPASy TrEMBL
Match: A0A6J1F557 (protochlorophyllide-dependent translocon component 52, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111440957 PE=4 SV=1)

HSP 1 Score: 910.6 bits (2352), Expect = 3.1e-261
Identity = 444/551 (80.58%), Postives = 474/551 (86.03%), Query Frame = 0

Query: 1   MDVLKFSSAPLHFTCKLNKSQFPKPCMQIIHLSSSSSSSINRHSRRLKLCASTSSAISAE 60
           MDVLKFSS PLHFT KL KS+F K C+Q+  L S  SSSI+RHSRR KL AS SS IS E
Sbjct: 1   MDVLKFSSVPLHFTAKLKKSRFLKQCLQVNLLPSLPSSSISRHSRRFKLRASVSSTISTE 60

Query: 61  --ESANSFEVEAESKRRE-ERFDWYAQWYPIMPICDLDQRVPHGKTVMGIDVVVWWDKNE 120
             E A+ FEVEAE++RRE ERFDWYAQWYPIMPICDLD+RVPHGKTVMGIDVVVWWDKNE
Sbjct: 61  EFEFASPFEVEAENERREDERFDWYAQWYPIMPICDLDKRVPHGKTVMGIDVVVWWDKNE 120

Query: 121 NAWKVFEDLCPHRLAPLSEGRIDQWGRLQCVYHGWCFNGSGDCKFIPQAPPDGPPVHTSK 180
           +AWKVFEDLCPHRLAPLSEGRIDQWGRLQCVYHGWCFNGSGDCKFIPQAPPDGPPVHTSK
Sbjct: 121 SAWKVFEDLCPHRLAPLSEGRIDQWGRLQCVYHGWCFNGSGDCKFIPQAPPDGPPVHTSK 180

Query: 181 RACVAVYPSIVQNGMLWFWPNSDPQYKDILVKKKPPYIPELDDPSYTKSICSRDLPYGVT 240
           RACVAVYPSIVQN +LWFWPNSDPQYKDILVKKKPPYIPELDDPSYTK I +RDLPYG  
Sbjct: 181 RACVAVYPSIVQNDILWFWPNSDPQYKDILVKKKPPYIPELDDPSYTKLISNRDLPYGYE 240

Query: 241 EVDLIVKDRKELECVEKTSMFTQLLCLFALSEKADREGGKPIEMVVRDLDVYGFITDQEQ 300
            +   + D   +       M  Q        EKADREGGKPIE+ ++DLD YGFI++QE 
Sbjct: 241 VLTENLMDPAHVPYAHYGIMGRQQP---NNREKADREGGKPIEIFIKDLDAYGFISNQEL 300

Query: 301 GNGQFFAPCVFYTSFSPRSKERNSKHQGNGTLLSTENNTVARNTSQRKTMLIFMCIPVSP 360
           GN QFFAPCVFYTSFS RSK+R  KHQ NG LLS ENN  A NTSQRK ML+FMCIPVSP
Sbjct: 301 GNSQFFAPCVFYTSFSLRSKKRTPKHQDNGALLSAENNAEAPNTSQRKAMLVFMCIPVSP 360

Query: 361 GKSRVIFIPLRNFAVWIDWFIPRWMFHVGQNLVFDSDLYLLHVEERKILDVGPLNWQKAC 420
           G+SR+IF+ LRNFA+ ID  IPRW+FHVGQNL+ DSDLYLLHVEERKILDVGP NWQKAC
Sbjct: 361 GRSRLIFVSLRNFAIGIDRIIPRWVFHVGQNLILDSDLYLLHVEERKILDVGPSNWQKAC 420

Query: 421 FVPTKSDAKVVAFRRWLNKYSDGHVDWRGKFSGILPPSPPREQLMDRYWSHVVNCSSCSV 480
           FVPTKSDAKVVAFRRWLNKYSDGHVDWRGKFSGILPPSPP+EQLMDRYWSHVVNCSSC+ 
Sbjct: 421 FVPTKSDAKVVAFRRWLNKYSDGHVDWRGKFSGILPPSPPKEQLMDRYWSHVVNCSSCNG 480

Query: 481 AYKGLNALEVVLQVISIASLGVFAVTKQSLVSGAARVGMVAMAVLCFAASRWLSHFIYKN 540
           AYKGLNALEVVLQVISIA LGVFA T QSLVS  AR+ MVAMAVLCFAASRWLSHFIYKN
Sbjct: 481 AYKGLNALEVVLQVISIALLGVFAATNQSLVSRTARIVMVAMAVLCFAASRWLSHFIYKN 540

Query: 541 FHFHDYNHALR 549
           FHFHDYNHALR
Sbjct: 541 FHFHDYNHALR 548

BLAST of Spg022565 vs. ExPASy TrEMBL
Match: A0A1S3BQM5 (protochlorophyllide-dependent translocon component 52, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103492435 PE=4 SV=1)

HSP 1 Score: 902.5 bits (2331), Expect = 8.6e-259
Identity = 433/548 (79.01%), Postives = 474/548 (86.50%), Query Frame = 0

Query: 1   MDVLKFSSAPLHFTCKLNKSQFPKPCMQIIHLSSSSSSSINRHSRRLKLCASTSSAISAE 60
           MD+LK SS+PLHFT K +K QFP  C++I    SSSSSSINR + R KLCA TSSAIS +
Sbjct: 1   MDLLKLSSSPLHFTPKFDKPQFPNQCLRIPLFPSSSSSSINRRNPRFKLCAITSSAISTQ 60

Query: 61  ESANSFEVEAESKRREERFDWYAQWYPIMPICDLDQRVPHGKTVMGIDVVVWWDKNENAW 120
           ESAN F+VEAES+  EERFDWYAQWYPIMPICDLD+RVPHGKTVMGIDVVVWWDKNE+AW
Sbjct: 61  ESANPFQVEAESESGEERFDWYAQWYPIMPICDLDKRVPHGKTVMGIDVVVWWDKNESAW 120

Query: 121 KVFEDLCPHRLAPLSEGRIDQWGRLQCVYHGWCFNGSGDCKFIPQAPPDGPPVHTSKRAC 180
           KVF+DLCPHRLAPLSEGRIDQWGRLQCVYHGWCFNGSGDCKFIPQAPPDGPPVHTSKRAC
Sbjct: 121 KVFDDLCPHRLAPLSEGRIDQWGRLQCVYHGWCFNGSGDCKFIPQAPPDGPPVHTSKRAC 180

Query: 181 VAVYPSIVQNGMLWFWPNSDPQYKDILVKKKPPYIPELDDPSYTKSICSRDLPYGVTEVD 240
           VAVYPSIVQN +LWFWPNSDPQYKDI+VKKKPPYIPELDDPS+TK I +RDLPYG   + 
Sbjct: 181 VAVYPSIVQNDVLWFWPNSDPQYKDIMVKKKPPYIPELDDPSFTKLISNRDLPYGYEILT 240

Query: 241 LIVKDRKELECVEKTSMFTQLLCLFALSEKADREGGKPIEMVVRDLDVYGFITDQEQGNG 300
             + D   +       M  Q        EKADREGGKPI++VVRDL++ GFI++Q+ G+ 
Sbjct: 241 ENLMDPAHVPYAHYGIMGRQQP---KNREKADREGGKPIDIVVRDLNINGFISNQDLGSS 300

Query: 301 QFFAPCVFYTSFSPRSKERNSKHQGNGTLLSTENNTVARNTSQRKTMLIFMCIPVSPGKS 360
           QFFAPCVF+TSFS  SK+ NS  Q +G LLS ENN VA NTSQRK MLIFMCIPV PGKS
Sbjct: 301 QFFAPCVFFTSFSLPSKKTNSNRQDSGALLSGENNAVAPNTSQRKAMLIFMCIPVGPGKS 360

Query: 361 RVIFIPLRNFAVWIDWFIPRWMFHVGQNLVFDSDLYLLHVEERKILDVGPLNWQKACFVP 420
           R+IF+ LRNFAV ID  IPRW+FHVGQNL+ DSDLYLLH+EERKILDVGP NWQKACFVP
Sbjct: 361 RLIFVSLRNFAVSIDRIIPRWIFHVGQNLILDSDLYLLHIEERKILDVGPSNWQKACFVP 420

Query: 421 TKSDAKVVAFRRWLNKYSDGHVDWRGKFSGILPPSPPREQLMDRYWSHVVNCSSCSVAYK 480
           TKSDAKVVAFRRWLN+YSDG VDWRGKF+GI PPSPP+EQLMDRYWSHVVNCSSC VAYK
Sbjct: 421 TKSDAKVVAFRRWLNRYSDGQVDWRGKFNGIFPPSPPKEQLMDRYWSHVVNCSSCKVAYK 480

Query: 481 GLNALEVVLQVISIASLGVFAVTKQSLVSGAARVGMVAMAVLCFAASRWLSHFIYKNFHF 540
           GLNALEVVLQVISIASLG+FA TKQSLVSGAARV MVAMA+LCFA+SRWLSHFIYKNFHF
Sbjct: 481 GLNALEVVLQVISIASLGIFAATKQSLVSGAARVLMVAMAILCFASSRWLSHFIYKNFHF 540

Query: 541 HDYNHALR 549
           HDYNHALR
Sbjct: 541 HDYNHALR 545

BLAST of Spg022565 vs. ExPASy TrEMBL
Match: A0A6J1HR92 (protochlorophyllide-dependent translocon component 52, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111466721 PE=4 SV=1)

HSP 1 Score: 902.1 bits (2330), Expect = 1.1e-258
Identity = 438/551 (79.49%), Postives = 473/551 (85.84%), Query Frame = 0

Query: 1   MDVLKFSSAPLHFTCKLNKSQFPKPCMQIIHLSSSSSSSINRHSRRLKLCASTSSAISAE 60
           MDVLKFSS PLHFT KLNKS+F K C+Q+  L S  SS I+RHSRR  L AS SS IS E
Sbjct: 1   MDVLKFSSVPLHFTAKLNKSRFLKQCLQVNLLPSLPSSLISRHSRRFNLRASVSSTISTE 60

Query: 61  --ESANSFEVEAESKRRE-ERFDWYAQWYPIMPICDLDQRVPHGKTVMGIDVVVWWDKNE 120
             E A+ FE EAE++RRE ERFDWYAQWYPIMPICDLD+RVPHGKTVMGIDVVVWWDKNE
Sbjct: 61  EFEYASPFEDEAENERREDERFDWYAQWYPIMPICDLDKRVPHGKTVMGIDVVVWWDKNE 120

Query: 121 NAWKVFEDLCPHRLAPLSEGRIDQWGRLQCVYHGWCFNGSGDCKFIPQAPPDGPPVHTSK 180
           +AWKVFED CPHRLAPLSEGRIDQWGRLQCVYHGWCFNGSGDCKFIPQAPPDGPPVHTSK
Sbjct: 121 SAWKVFEDSCPHRLAPLSEGRIDQWGRLQCVYHGWCFNGSGDCKFIPQAPPDGPPVHTSK 180

Query: 181 RACVAVYPSIVQNGMLWFWPNSDPQYKDILVKKKPPYIPELDDPSYTKSICSRDLPYGVT 240
           RACVAVYPSIVQN +LWFWPNSDPQYKDILVKKKPPYIPELDDPSYTK   +RDLPYG  
Sbjct: 181 RACVAVYPSIVQNDILWFWPNSDPQYKDILVKKKPPYIPELDDPSYTKLTSNRDLPYG-- 240

Query: 241 EVDLIVKDRKELECVEKTSMFTQLLCLFALSEKADREGGKPIEMVVRDLDVYGFITDQEQ 300
             +++ ++  +   V         +      EKADREGGKPIE+ V+DLD YGFI++QE 
Sbjct: 241 -YEILTENLMDPAHVPYAHYGIMGMRQPNNREKADREGGKPIEIFVKDLDAYGFISNQEL 300

Query: 301 GNGQFFAPCVFYTSFSPRSKERNSKHQGNGTLLSTENNTVARNTSQRKTMLIFMCIPVSP 360
           GN QFFAPCVFYTSFS RSK+R  KHQ NG LLS ENN  A NTSQRK ML+FMCIPVSP
Sbjct: 301 GNSQFFAPCVFYTSFSLRSKKRTPKHQDNGALLSAENNAEAPNTSQRKAMLVFMCIPVSP 360

Query: 361 GKSRVIFIPLRNFAVWIDWFIPRWMFHVGQNLVFDSDLYLLHVEERKILDVGPLNWQKAC 420
           G+SR+IF+ LRNFA+ ID  IPRW+FHVGQNL+ DSDLYLLH+EERKILDVGP NWQKAC
Sbjct: 361 GRSRLIFVSLRNFAIGIDRIIPRWVFHVGQNLILDSDLYLLHLEERKILDVGPSNWQKAC 420

Query: 421 FVPTKSDAKVVAFRRWLNKYSDGHVDWRGKFSGILPPSPPREQLMDRYWSHVVNCSSCSV 480
           FVPTKSDAKVVAFRRWLNKYSDGHVDWRGKFSGILPPSPP+EQLMDRYWSHVVNCSSC+ 
Sbjct: 421 FVPTKSDAKVVAFRRWLNKYSDGHVDWRGKFSGILPPSPPKEQLMDRYWSHVVNCSSCNG 480

Query: 481 AYKGLNALEVVLQVISIASLGVFAVTKQSLVSGAARVGMVAMAVLCFAASRWLSHFIYKN 540
           AYKGLNALEVVLQVISIA LGVFA T QSLVSG AR+ MVAMAVLCFAASRWLSHFIYKN
Sbjct: 481 AYKGLNALEVVLQVISIALLGVFAATNQSLVSGTARIVMVAMAVLCFAASRWLSHFIYKN 540

Query: 541 FHFHDYNHALR 549
           FHFHDYNHA+R
Sbjct: 541 FHFHDYNHAIR 548

BLAST of Spg022565 vs. ExPASy TrEMBL
Match: A0A6J1D0M6 (protochlorophyllide-dependent translocon component 52, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111016497 PE=4 SV=1)

HSP 1 Score: 902.1 bits (2330), Expect = 1.1e-258
Identity = 436/550 (79.27%), Postives = 476/550 (86.55%), Query Frame = 0

Query: 1   MDVLKFSSAPLHFTCKLNKSQFPKPCMQIIHLSSSSSSSINRHSRRLKLCASTSSAISAE 60
           M+VLKFSSAPLH T K+NK +F   C Q+  L S+SS +I+RHSRR KLCA++S  IS  
Sbjct: 8   MEVLKFSSAPLHSTAKVNKYRFRNQCFQVHLLPSTSSLTIHRHSRRFKLCANSSPTIST- 67

Query: 61  ESANSFEVEAESKRREERFDWYAQWYPIMPICDLDQRVPHGKTVMGIDVVVWWDKNENAW 120
           ESAN  EVE ES+RREERFDWYAQWYP+MPICDLD+RVPHGK VMGIDVVVWWD+NE+AW
Sbjct: 68  ESANPLEVEPESERREERFDWYAQWYPVMPICDLDKRVPHGKKVMGIDVVVWWDRNESAW 127

Query: 121 KVFEDLCPHRLAPLSEGRIDQWGRLQCVYHGWCFNGSGDCKFIPQAPPDGPPVHTSKRAC 180
           KVF+D CPHRLAPLSEGRIDQWGRLQCVYHGWCFNGSG CKFIPQAPPDGPPVHTSKRAC
Sbjct: 128 KVFDDACPHRLAPLSEGRIDQWGRLQCVYHGWCFNGSGGCKFIPQAPPDGPPVHTSKRAC 187

Query: 181 VAVYPSIVQNGMLWFWPNSDPQYKDILVKKKPPYIPELDDPSYTKSICSRDLPYGVTEVD 240
           VAVYPSIVQN +LWFWPNSDPQYKDILVKKKPPYIPELDDPSYTKS+C+RDLPYG   + 
Sbjct: 188 VAVYPSIVQNDILWFWPNSDPQYKDILVKKKPPYIPELDDPSYTKSMCNRDLPYGYEVLI 247

Query: 241 LIVKDRKELECVEKTSMFTQLLCLFALSEKADREGGKPIEMVVRDLDVYGFITDQEQGNG 300
             + D   +       M TQ       SEKADREGGKPIE+VVRDLD+YGFI++Q  G+ 
Sbjct: 248 ENLMDPAHVPYAHYGIMGTQQP---KNSEKADREGGKPIEIVVRDLDIYGFISNQTLGSS 307

Query: 301 QFFAPCVFYTSFS--PRSKERNSKHQGNGTLLSTENNTVARNTSQRKTMLIFMCIPVSPG 360
           +FFAPC++Y+SFS  P SKE+N K Q NGTLLS ENNTV  NTSQ+K MLIFMCIPVSPG
Sbjct: 308 KFFAPCLYYSSFSLGPISKEKNPKLQDNGTLLSAENNTVTPNTSQKKGMLIFMCIPVSPG 367

Query: 361 KSRVIFIPLRNFAVWIDWFIPRWMFHVGQNLVFDSDLYLLHVEERKILDVGPLNWQKACF 420
           KSRVIF+  RNFAVW+D  IPRWMFHVGQNL+ DSDLYLLHVEERKILD+GPLNWQKACF
Sbjct: 368 KSRVIFVFPRNFAVWMDRIIPRWMFHVGQNLILDSDLYLLHVEERKILDIGPLNWQKACF 427

Query: 421 VPTKSDAKVVAFRRWLNKYSDGHVDWRGKFSGILPPSPPREQLMDRYWSHVVNCSSCSVA 480
           VPTKSDA VVAFRRWLNKYSDGHVDWRGKFSG LPPSPP+EQLMDRYWSHVVNCSSC VA
Sbjct: 428 VPTKSDAIVVAFRRWLNKYSDGHVDWRGKFSGSLPPSPPKEQLMDRYWSHVVNCSSCRVA 487

Query: 481 YKGLNALEVVLQVISIASLGVFAVTKQSLVSGAARVGMVAMAVLCFAASRWLSHFIYKNF 540
            KGLNALEVVLQVISIASLG+FA TKQSLVS AAR  MVAMA+LCFAASRWLSH+IYKNF
Sbjct: 488 CKGLNALEVVLQVISIASLGIFASTKQSLVSAAARNVMVAMAILCFAASRWLSHYIYKNF 547

Query: 541 HFHDYNHALR 549
           HFHDYNHALR
Sbjct: 548 HFHDYNHALR 553

BLAST of Spg022565 vs. ExPASy TrEMBL
Match: A0A1S3BQK3 (protochlorophyllide-dependent translocon component 52, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103492436 PE=4 SV=1)

HSP 1 Score: 878.2 bits (2268), Expect = 1.7e-251
Identity = 422/550 (76.73%), Postives = 467/550 (84.91%), Query Frame = 0

Query: 1   MDVLKFSSAPLHFTCKLNKSQFPKPCMQIIHLSSSSSSSINRHSRRLKLCASTSSAISAE 60
           MDVLK SS+PLHFT KL+KSQFPK CMQI    SSS SSINR +R  KLCA+T    S E
Sbjct: 3   MDVLKLSSSPLHFTTKLSKSQFPKQCMQIPLFPSSSFSSINRSNRSFKLCATT---FSTE 62

Query: 61  ESANSFEVEAESKRREERFDWYAQWYPIMPICDLDQRVPHGKTVMGIDVVVWWDKNENAW 120
           E+ N FEVEA+S+R   RFDWYAQWYPIMPICDLD+RVP GKTVMGIDVVVWWDKNE+ W
Sbjct: 63  ETGNPFEVEAQSER---RFDWYAQWYPIMPICDLDKRVPQGKTVMGIDVVVWWDKNESGW 122

Query: 121 KVFEDLCPHRLAPLSEGRIDQWGRLQCVYHGWCFNGSGDCKFIPQ--APPDGPPVHTSKR 180
           KVF+DLCPHRLAPLSEGRID+WGRLQCV+HGWCFNGSGDCKFIPQ  APPDGPPVHTSKR
Sbjct: 123 KVFDDLCPHRLAPLSEGRIDEWGRLQCVHHGWCFNGSGDCKFIPQAPAPPDGPPVHTSKR 182

Query: 181 ACVAVYPSIVQNGMLWFWPNSDPQYKDILVKKKPPYIPELDDPSYTKSICSRDLPYGVTE 240
           ACV+VYPS+VQN +LWFWPNSDPQYKDI+ KK PP+IPELDDPSYTK I SRDLPYG   
Sbjct: 183 ACVSVYPSVVQNDILWFWPNSDPQYKDIMAKKTPPFIPELDDPSYTKLIISRDLPYGYEV 242

Query: 241 VDLIVKDRKELECVEKTSMFTQLLCLFALSEKADREGGKPIEMVVRDLDVYGFITDQEQG 300
           +   + D   +       +  Q        +KADREGG+PIE+VVRDLDV+GFI++Q  G
Sbjct: 243 LTENLMDPAHVPYAHYGIVSRQQ----TKHKKADREGGQPIEIVVRDLDVHGFISNQGLG 302

Query: 301 NGQFFAPCVFYTSFSPRSKERNSKHQGNGTLLSTENNTVARNTSQRKTMLIFMCIPVSPG 360
           + +F APCV YTSF+  SKERN+KHQ NGTL   ENN VA  TSQRK M +FMCIP+SPG
Sbjct: 303 HSKFVAPCVLYTSFNLPSKERNAKHQDNGTLSLGENNAVASKTSQRKRMAVFMCIPISPG 362

Query: 361 KSRVIFIPLRNFAVWIDWFIPRWMFHVGQNLVFDSDLYLLHVEERKILDVGPLNWQKACF 420
           KSRVIFIPLRNFA+W++  IPRWMFH+GQNLV DSD YLLH+EERKILDVG  NWQKACF
Sbjct: 363 KSRVIFIPLRNFAIWVNQIIPRWMFHLGQNLVLDSDSYLLHLEERKILDVGTANWQKACF 422

Query: 421 VPTKSDAKVVAFRRWLNKYSDGHVDWRGKFSGILPPSPPREQLMDRYWSHVVNCSSCSVA 480
           +PTKSDAKVVAFRRWLNKYSDGHV WRGKFSGILPPSPP+EQLM+RYWSHVVNCSSC VA
Sbjct: 423 LPTKSDAKVVAFRRWLNKYSDGHVGWRGKFSGILPPSPPKEQLMERYWSHVVNCSSCKVA 482

Query: 481 YKGLNALEVVLQVISIASLGVFAVTKQSLVSGAARVGMVAMAVLCFAASRWLSHFIYKNF 540
           YKGLNALEVVLQVISIASLG+FA TKQSLVSGAARV MVAMA+LCFA+SRWLSHFIYKNF
Sbjct: 483 YKGLNALEVVLQVISIASLGIFAATKQSLVSGAARVLMVAMAILCFASSRWLSHFIYKNF 542

Query: 541 HFHDYNHALR 549
           HFHDYNHA+R
Sbjct: 543 HFHDYNHAIR 542

BLAST of Spg022565 vs. TAIR 10
Match: AT4G25650.2 (ACD1-like )

HSP 1 Score: 644.0 bits (1660), Expect = 1.1e-184
Identity = 315/541 (58.23%), Postives = 390/541 (72.09%), Query Frame = 0

Query: 32  LSSSSSSSINRHSRRLKLCASTSSAISAEESANS-FEVEAESKRREERFDWYAQWYPIMP 91
           L  S +S I R S R     S+  + SA  S NS  E EA  +   ++FDWYA WYP+MP
Sbjct: 31  LPISPNSLITRKSSRFTTAVSSPPSSSAATSTNSPPEPEALFEPGSDKFDWYANWYPVMP 90

Query: 92  ICDLDQRVPHGKTVMGIDVVVWWDKNENAWKVFEDLCPHRLAPLSEGRIDQWGRLQCVYH 151
           ICDLD++VPHGK VMGID+VVWWD+NE  WKV +D CPHRLAPLS+GRIDQWGRLQCVYH
Sbjct: 91  ICDLDKKVPHGKKVMGIDLVVWWDRNEKQWKVMDDTCPHRLAPLSDGRIDQWGRLQCVYH 150

Query: 152 GWCFNGSGDCKFIPQAPPDGPPVHTSKRACVAVYPSIVQNGMLWFWPNSDPQYKDILVKK 211
           GWCFNGSGDCK IPQAPPDGPPVHT K+ACVAVYPS VQ+ ++WFWPNSDP+YK+I+   
Sbjct: 151 GWCFNGSGDCKLIPQAPPDGPPVHTFKQACVAVYPSTVQHEIIWFWPNSDPKYKNIIETN 210

Query: 212 KPPYIPELDDPSYTKSICSRDLPYGVTEVDLIVKD---------------------RKEL 271
           KPPYIPEL+DPS+TK + +RD+PYG    D++V++                      K +
Sbjct: 211 KPPYIPELEDPSFTKLMGNRDIPYG---YDVLVENLMDPAHVPYAHYGLMRFPKPKGKYI 270

Query: 272 ECVEKT--SMFTQLLCLFALSEKADREGGKPIEMVVRDLDVYGFITDQEQGNGQFFAPCV 331
            C+  +  + FT L  L  L+EK DREGGKP+E+ V+ LD  GF + QE G   F APCV
Sbjct: 271 ICISNSCFNPFTNLQIL--LAEKIDREGGKPLEINVKKLDNKGFFSKQEWGYSNFIAPCV 330

Query: 332 FYTSFSPRSKERNSKHQGNGTLLSTENNTVARNTSQRKTMLIFMCIPVSPGKSRVIFIPL 391
           + +S  P  ++   +H+      S +        S+R+  LIF+CIPVSPG+SR+I+   
Sbjct: 331 YRSSTDPLPEQ---EHEYPAPAASDK-----AALSKRRLSLIFICIPVSPGRSRLIWTFP 390

Query: 392 RNFAVWIDWFIPRWMFHVGQNLVFDSDLYLLHVEERKILDVGPLNWQKACFVPTKSDAKV 451
           RNF V+ID  +PRW+FH+GQN + DSDL+LLHVEERKIL+ GP NWQKACF+PTKSDA V
Sbjct: 391 RNFGVFIDKIVPRWVFHIGQNTILDSDLHLLHVEERKILERGPENWQKACFIPTKSDANV 450

Query: 452 VAFRRWLNKYSDGHVDWRGKFSG-ILPPSPPREQLMDRYWSHVVNCSSCSVAYKGLNALE 511
           V FRRW NKYS+  VDWRGKF   +LPP+PPREQL DRYWSHV NCSSC  A+K LNALE
Sbjct: 451 VTFRRWFNKYSEARVDWRGKFDPFLLPPTPPREQLFDRYWSHVENCSSCKKAHKYLNALE 510

Query: 512 VVLQVISIASLGVFAVTKQSLVSGAARVGMVAMAVLCFAASRWLSHFIYKNFHFHDYNHA 548
           V+LQ+ S+A +GV AV KQ+ +S  AR+ ++  AVL FAAS+WLSHFIYK FH+HDYNHA
Sbjct: 511 VILQIASVAMIGVMAVLKQTTMSNVARIAVLVAAVLSFAASKWLSHFIYKTFHYHDYNHA 558

BLAST of Spg022565 vs. TAIR 10
Match: AT4G25650.1 (ACD1-like )

HSP 1 Score: 642.5 bits (1656), Expect = 3.1e-184
Identity = 310/518 (59.85%), Postives = 382/518 (73.75%), Query Frame = 0

Query: 32  LSSSSSSSINRHSRRLKLCASTSSAISAEESANS-FEVEAESKRREERFDWYAQWYPIMP 91
           L  S +S I R S R     S+  + SA  S NS  E EA  +   ++FDWYA WYP+MP
Sbjct: 31  LPISPNSLITRKSSRFTTAVSSPPSSSAATSTNSPPEPEALFEPGSDKFDWYANWYPVMP 90

Query: 92  ICDLDQRVPHGKTVMGIDVVVWWDKNENAWKVFEDLCPHRLAPLSEGRIDQWGRLQCVYH 151
           ICDLD++VPHGK VMGID+VVWWD+NE  WKV +D CPHRLAPLS+GRIDQWGRLQCVYH
Sbjct: 91  ICDLDKKVPHGKKVMGIDLVVWWDRNEKQWKVMDDTCPHRLAPLSDGRIDQWGRLQCVYH 150

Query: 152 GWCFNGSGDCKFIPQAPPDGPPVHTSKRACVAVYPSIVQNGMLWFWPNSDPQYKDILVKK 211
           GWCFNGSGDCK IPQAPPDGPPVHT K+ACVAVYPS VQ+ ++WFWPNSDP+YK+I+   
Sbjct: 151 GWCFNGSGDCKLIPQAPPDGPPVHTFKQACVAVYPSTVQHEIIWFWPNSDPKYKNIIETN 210

Query: 212 KPPYIPELDDPSYTKSICSRDLPYGVTEVDLIVKDRKELECVEKTSMFTQLLCLFALSEK 271
           KPPYIPEL+DPS+TK + +RD+PYG    D++V++  +   V        L+      EK
Sbjct: 211 KPPYIPELEDPSFTKLMGNRDIPYG---YDVLVENLMDPAHVPYAHY--GLMRFPKPKEK 270

Query: 272 ADREGGKPIEMVVRDLDVYGFITDQEQGNGQFFAPCVFYTSFSPRSKERNSKHQGNGTLL 331
            DREGGKP+E+ V+ LD  GF + QE G   F APCV+ +S  P  ++   +H+      
Sbjct: 271 IDREGGKPLEINVKKLDNKGFFSKQEWGYSNFIAPCVYRSSTDPLPEQ---EHEYPAPAA 330

Query: 332 STENNTVARNTSQRKTMLIFMCIPVSPGKSRVIFIPLRNFAVWIDWFIPRWMFHVGQNLV 391
           S +        S+R+  LIF+CIPVSPG+SR+I+   RNF V+ID  +PRW+FH+GQN +
Sbjct: 331 SDK-----AALSKRRLSLIFICIPVSPGRSRLIWTFPRNFGVFIDKIVPRWVFHIGQNTI 390

Query: 392 FDSDLYLLHVEERKILDVGPLNWQKACFVPTKSDAKVVAFRRWLNKYSDGHVDWRGKFSG 451
            DSDL+LLHVEERKIL+ GP NWQKACF+PTKSDA VV FRRW NKYS+  VDWRGKF  
Sbjct: 391 LDSDLHLLHVEERKILERGPENWQKACFIPTKSDANVVTFRRWFNKYSEARVDWRGKFDP 450

Query: 452 -ILPPSPPREQLMDRYWSHVVNCSSCSVAYKGLNALEVVLQVISIASLGVFAVTKQSLVS 511
            +LPP+PPREQL DRYWSHV NCSSC  A+K LNALEV+LQ+ S+A +GV AV KQ+ +S
Sbjct: 451 FLLPPTPPREQLFDRYWSHVENCSSCKKAHKYLNALEVILQIASVAMIGVMAVLKQTTMS 510

Query: 512 GAARVGMVAMAVLCFAASRWLSHFIYKNFHFHDYNHAL 548
             AR+ ++  AVL FAAS+WLSHFIYK FH+HDYNHA+
Sbjct: 511 NVARIAVLVAAVLSFAASKWLSHFIYKTFHYHDYNHAV 535

BLAST of Spg022565 vs. TAIR 10
Match: AT3G44880.1 (Pheophorbide a oxygenase family protein with Rieske [2Fe-2S] domain )

HSP 1 Score: 236.5 bits (602), Expect = 5.1e-62
Identity = 174/545 (31.93%), Postives = 245/545 (44.95%), Query Frame = 0

Query: 34  SSSSSSINRHSRRLKLCASTSSAISAEESANSFEVEAESKRREERFDWYAQWYPIMPICD 93
           S S S +  +  R+    S  ++ S EE     E   + +     F W   WYP+  + D
Sbjct: 37  SPSRSKLFHNPLRVAAPPSVPTSDSTEEKRIEEEYGGDKEEEGSEFKWRDHWYPVSLVED 96

Query: 94  LDQRVPHGKTVMGIDVVVWWDKNENAWKVFEDLCPHRLAPLSEGRIDQWGRLQCVYHGWC 153
           LD  VP    ++G D+V+W+D+N+  W  F+DLCPHRLAPLSEGR+D+ G LQC YHGW 
Sbjct: 97  LDPNVPTPFQLLGRDLVLWFDRNDQKWAAFDDLCPHRLAPLSEGRLDENGHLQCSYHGWS 156

Query: 154 FNGSGDCKFIPQAPPDGPPVHT--SKRACVAVYPSIVQNGMLWFWPNSDPQYKDILVKKK 213
           F G G C  IPQA   GP      S RAC   +P++V  G+L+ WP  D    D     +
Sbjct: 157 FGGCGSCTRIPQAATSGPEARAVKSPRACAIKFPTMVSQGLLFVWP--DENGWDRANSIE 216

Query: 214 PPYIP-ELDDPSYTKSICSRDLPYGVTEVDLIVKDRKELECVEKTSMFTQLLCLFALSEK 273
           PP +P + D P ++     RDL YG   +   V D   ++                    
Sbjct: 217 PPRLPDDFDKPEFSTVTIQRDLFYGYDTLMENVSDPSHIDFAHH-------------KVT 276

Query: 274 ADREGGKPIEMVVRDLDVYGF---ITDQEQGNGQFFAPCVFYTSFSPRSKERNSKHQGNG 333
             R+  KP+   V     +GF     D  +   +F APC     +S    E ++K    G
Sbjct: 277 GRRDRAKPLPFKVESSGPWGFQGANDDSPRITAKFVAPC-----YSMNKIELDAKLPIVG 336

Query: 334 TLLSTENNTVARNTSQRKTMLIFMC---IPVSPGKSRVIFIPLRNF--------AVWIDW 393
                            +  +I++C   IP++PGK+R I    RNF        A W   
Sbjct: 337 ----------------NQKWVIWICSFNIPMAPGKTRSIVCSARNFFQFSVPGPAWW--Q 396

Query: 394 FIPRWMFHVGQNLVFDSDLYLLHVEERKIL---------DVGPLNWQKACFVPTKSDAKV 453
            +PRW  H   NLV+D D+ +L  +E+  L         DV    + K  F PT++D  V
Sbjct: 397 VVPRWYEHWTSNLVYDGDMIVLQGQEKVFLAKSMESPDYDVNK-QYTKLTFTPTQADRFV 456

Query: 454 VAFRRWLNKYSDGHVDWRGKFSGILP-PSP--PREQLMDRYWSHVVNCSSCSVAYKGLNA 513
           +AFR WL ++     +W G      P PS    + Q++DR+  H   CSSC  AY     
Sbjct: 457 LAFRNWLRRHGKSQPEWFGSTPSNQPLPSTVLTKRQMLDRFDQHTQVCSSCKGAYNSFQI 516

Query: 514 LEVVL---QVISIASLGVFAVTKQSLVSGAARVGMVAMAVLCFAASRWLSHFIYKNFHFH 547
           L+  L    V   A+ GV +  +  LV        +A   L  AAS +  H   KNF F 
Sbjct: 517 LKKFLVGATVFWAATAGVPSDVQIRLV--------LAGLSLISAASAYALHEQEKNFVFR 534

BLAST of Spg022565 vs. TAIR 10
Match: AT2G24820.1 (translocon at the inner envelope membrane of chloroplasts 55-II )

HSP 1 Score: 149.1 bits (375), Expect = 1.1e-35
Identity = 136/534 (25.47%), Postives = 221/534 (41.39%), Query Frame = 0

Query: 45  RRLKLCASTSSAISAE-ESANSFEVEAESKRREE--RFDWYAQWYPIMPICDLDQRVPHG 104
           R L+  A   +A+S + E      +  E ++R E   +DW  +WYP+    ++ +  P G
Sbjct: 45  RLLRRSAVAGTAVSDQTEGGGDVLLNPEEEKRVEVADYDWTEEWYPLYLTKNVPEDAPLG 104

Query: 105 KTVMGIDVVVWWDKNENAWKVFEDLCPHRLAPLSEGRIDQWGRLQCVYHGWCFNGSGDCK 164
            TV    +V++ D  E   + +ED CPHRLA LSEG++   GRL+C+YHGW F G G C 
Sbjct: 105 LTVYDRQIVLYKD-GEGTLRCYEDRCPHRLAKLSEGQLID-GRLECLYHGWQFEGEGKCV 164

Query: 165 FIPQAPPDGPPVHTSKRACVAVYPSIVQNGMLWFWPNSDPQYKDILVKKKPPYIPELDDP 224
            IPQ P         K ACV  Y      G++W W ++    K     +K P+      P
Sbjct: 165 KIPQLPAS---AKIPKAACVKTYEVKDSQGVVWVWMST----KTPPNPEKLPWFENFARP 224

Query: 225 SYTKSICSRDLPYGVTEVDLIVKDRKELECVEKTSMFTQLLCLFALSEKADREGGKPIEM 284
            +     + +LPY  + +   + D   +      + FT           A RE  +P+  
Sbjct: 225 GFFDISTTHELPYDHSILLENLMDPAHVPISHDRTDFT-----------AKREDAQPLVF 284

Query: 285 VVRDLDVYGFI----TDQEQGNG----QFFAPCVFYTSFSPRSKERNSKHQGNGTLLSTE 344
            V +    GF      ++E G G    +F APCV          + N + +G   + +  
Sbjct: 285 EVTERSNRGFAGTWGREKEGGKGSNLLRFDAPCVL---------QNNREFEGKDGVKNYF 344

Query: 345 NNTVARNTSQRKTMLIFMCIPVSPGKSRVIF---IPLRNFAVWIDWFIPRWMFHVGQNLV 404
           +              +F+C P   GKS +I    +  R+  V +   +P+W +H     V
Sbjct: 345 SG-------------LFLCRPTGQGKSMLIVRFGVTKRSPLVSV---LPQWFWHQNACKV 404

Query: 405 FDSDLYLLHVEERKILDVGPLNWQKACFVPTK--------SDAKVVAFRRWLNKYSDGHV 464
           F+ D+  L  +   ++       +K   VPTK        SD  V  +R+W++K   G  
Sbjct: 405 FEQDMGFLSSQNEVLMK------EK---VPTKDLYLNLKSSDTWVAEYRKWMDKVGHGMP 464

Query: 465 DWRGKFSGILPPSPP---------------------------REQLMDRYWSHVVNCSSC 524
              G  +  LP  PP                              L +RY+ H+++C SC
Sbjct: 465 YHFGHRTISLPKVPPVVEHAPAGLIAALSASYPAKGGIGTMHAPNLANRYFRHIIHCRSC 518

Query: 525 SVAYKGLNALEVVLQVISIA--SLGVFAVTKQSLVSGAARVGMVAMAVLCFAAS 528
           S   K     + +L   ++A  +L +  V++Q       +  ++  A LC AA+
Sbjct: 525 SNVIKSFELWKNILSATAVALTALAILVVSRQ------WKAVLLGSAALCSAAA 518

BLAST of Spg022565 vs. TAIR 10
Match: AT1G44446.1 (Pheophorbide a oxygenase family protein with Rieske [2Fe-2S] domain )

HSP 1 Score: 72.8 bits (177), Expect = 9.7e-13
Identity = 41/148 (27.70%), Postives = 60/148 (40.54%), Query Frame = 0

Query: 85  WYPIMPICDLDQRVPHGKTVMGIDVVVWWDKNENAWKVF----------EDLCPHRLAPL 144
           WYP+    DL             D +V  +  E  W +F           + C HR  PL
Sbjct: 221 WYPVAFTADLKH-----------DTMVPIECFEQPWVIFRGEDGKPGCVRNTCAHRACPL 280

Query: 145 SEGRIDQWGRLQCVYHGWCFNGSGDCKFIPQAPPDGPPVHTSKRACVAVYPSIVQNGMLW 204
             G +++ GR+QC YHGW ++  G+CK +P             +  +   P + Q GM+W
Sbjct: 281 DLGTVNE-GRIQCPYHGWEYSTDGECKKMPST--------KLLKVKIKSLPCLEQEGMIW 339

Query: 205 FWPNSDPQYKDILVKKKPPYIPELDDPS 223
            WP  +P           P +P L  PS
Sbjct: 341 IWPGDEP---------PAPILPSLQPPS 339