Spg022559 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg022559 vs. NCBI nr
Match: XP_008463375.1 (PREDICTED: probable sulfate transporter 3.5 [Cucumis melo])

HSP 1 Score: 1091.6 bits (2822), Expect = 0.0e+00
Identity = 571/694 (82.28%), Postives = 610/694 (87.90%), Query Frame = 0

Query: 1   MGSFKSDQKVSFADPRPFGKKLKSDLKETFFPDDPFRQFQDDR-ALHRVKKGVQYFIPIL 60
           MGS K+ Q V+F+ PRPFGK+LKSDLKETFFPDDPFRQF+D+R A+HR KKG QYFIPIL
Sbjct: 1   MGSLKNGQNVNFSAPRPFGKRLKSDLKETFFPDDPFRQFRDERGAMHRAKKGFQYFIPIL 60

Query: 61  DWLPKYNFNMFKYDLLAGITITSLAIPQGISYAKLGSLPPIIGLYSSFVPPLVYAVFGSS 120
           DWLPKYN NMFKYDLLAGITITSLAIPQGISYAKLG LPPIIGLYSSFVPPL+YAVFGSS
Sbjct: 61  DWLPKYNLNMFKYDLLAGITITSLAIPQGISYAKLGVLPPIIGLYSSFVPPLIYAVFGSS 120

Query: 121 KHLAVGTVAACSLLIADIIGAVASPEKEPTLYLHLVYTATFITGVFQAALGFLRLGILVD 180
           KHLAVGTVAACSLLIA+ IG VASPE+EPTLYLHLV+TATF+TG+ QA LGFLRLGILVD
Sbjct: 121 KHLAVGTVAACSLLIAETIGGVASPEEEPTLYLHLVFTATFVTGIMQAVLGFLRLGILVD 180

Query: 181 FLSHSTILGFMGGTAVIICLQQLKGIFGLTHFTSKTDVVSVLHAVISLRYEWKWQSAVVG 240
           FLSHSTILGFMGGTAVIICLQQLKGIFGLTHFTSKTDV SVLHAV SLR EWKW+SAVVG
Sbjct: 181 FLSHSTILGFMGGTAVIICLQQLKGIFGLTHFTSKTDVYSVLHAVFSLRKEWKWESAVVG 240

Query: 241 VIFLIFLQFTRFLRNRNPKLFWVSAMAPMVTVIIGCLFAYFIKGEKHGILTAVWDVGIDW 300
           VIFL+FLQFTR+LRNRNPKLFWVSAMAPMVTVIIGCLFAYFIKG +HGILT         
Sbjct: 241 VIFLLFLQFTRYLRNRNPKLFWVSAMAPMVTVIIGCLFAYFIKGSQHGILT--------- 300

Query: 301 VGMESHVIPMFGAPDLSGVGMELWTCGFQPNSHILVGHLSKGINPISIKFLNFDSKYISA 360
                                              VGHLSKGINPISI FLNFDSKY+SA
Sbjct: 301 -----------------------------------VGHLSKGINPISIHFLNFDSKYLSA 360

Query: 361 VLQAGLITGMVALAEGIAIGRSFAIMKNEQIDGNKEMVAFGLMNIVGSFTSCYLTTGPFS 420
           V+Q GLITG+VALAEGIAIGRSFAIMKNEQIDGNKEM+AFGLMNIVGSFTSCYLTTGPFS
Sbjct: 361 VVQTGLITGLVALAEGIAIGRSFAIMKNEQIDGNKEMIAFGLMNIVGSFTSCYLTTGPFS 420

Query: 421 KTAVNFNAGCRTAMSNIVMAVFMALTLLFLAPLFSYTPLVALSAIIMSAMLGLIKYEEMY 480
           KTAVNFNAGCRTAMSN+VMA+FMALTL+FLAP+FSYTPLVALSAIIMSAMLGLIKYEEMY
Sbjct: 421 KTAVNFNAGCRTAMSNVVMAIFMALTLIFLAPVFSYTPLVALSAIIMSAMLGLIKYEEMY 480

Query: 481 HIFIVDKFDFCICMAAFLGVALLSMDVGIMLSVGLALLRALLYMARPATCKLGKIPNSAL 540
           H+  VDKFDFCICMAAFLGVA LSMDVGIMLSVGLALLRALLYMARPATCKLGKIPNS+L
Sbjct: 481 HLLKVDKFDFCICMAAFLGVAFLSMDVGIMLSVGLALLRALLYMARPATCKLGKIPNSSL 540

Query: 541 YRDMEQYPDSNRVPGIIVLQLGSPVYYANSNYIRERIFRWIRDEQAMFGSIDGSVQHVLL 600
           YRD+EQYP++ R  GIIVLQLGSP+YYANSNYIRERIFRW+RDEQ    S DG V+HVLL
Sbjct: 541 YRDVEQYPNATRTHGIIVLQLGSPIYYANSNYIRERIFRWVRDEQG--DSKDGCVEHVLL 600

Query: 601 ELSGVTSIDMTGIETLVEIRRSLQAMGIQMGIVNPRIVVMEKMIASEFTDTIGKENIYLS 660
           ELSGVTSIDMTGIETL+EIRRSLQA GIQMGIVNPRIVVMEKMIASEFT TIGKENIYLS
Sbjct: 601 ELSGVTSIDMTGIETLIEIRRSLQANGIQMGIVNPRIVVMEKMIASEFTKTIGKENIYLS 648

Query: 661 VDDGVERCRDTVVKPKKTDASERSSDVTTMEQRV 694
           VD+GVERCRD V K K+T+ S  SS+V TMEQ+V
Sbjct: 661 VDEGVERCRDLVPKLKQTETSPGSSNVRTMEQQV 648

BLAST of Spg022559 vs. NCBI nr
Match: XP_031737048.1 (probable sulfate transporter 3.5 isoform X2 [Cucumis sativus])

HSP 1 Score: 1072.4 bits (2772), Expect = 1.6e-309
Identity = 561/694 (80.84%), Postives = 605/694 (87.18%), Query Frame = 0

Query: 1   MGSFKSDQKVSFADPRPFGKKLKSDLKETFFPDDPFRQFQDDR-ALHRVKKGVQYFIPIL 60
           MGS K+ + V+F+ PRPFGKKLKSDLKETFFPDDPF+QF+D+  A+ RVKKG QYFIPIL
Sbjct: 1   MGSLKNGENVNFSAPRPFGKKLKSDLKETFFPDDPFKQFRDESGAMDRVKKGFQYFIPIL 60

Query: 61  DWLPKYNFNMFKYDLLAGITITSLAIPQGISYAKLGSLPPIIGLYSSFVPPLVYAVFGSS 120
            WLPKYN NMFKYDLLAGITITSLAIPQGISYAKLG LPPIIGLYSSFVPPLVYAVFGSS
Sbjct: 61  QWLPKYNLNMFKYDLLAGITITSLAIPQGISYAKLGILPPIIGLYSSFVPPLVYAVFGSS 120

Query: 121 KHLAVGTVAACSLLIADIIGAVASPEKEPTLYLHLVYTATFITGVFQAALGFLRLGILVD 180
           KHLAVGTVAACSLLI++ IGAVASPE+EPTLYLHLV+TATF+TGV QA LGFLRLGILVD
Sbjct: 121 KHLAVGTVAACSLLISETIGAVASPEEEPTLYLHLVFTATFVTGVMQAVLGFLRLGILVD 180

Query: 181 FLSHSTILGFMGGTAVIICLQQLKGIFGLTHFTSKTDVVSVLHAVISLRYEWKWQSAVVG 240
           FLSHSTILGFMGGTAVIICLQQLKGIFGLTHFTSKTDV SVLHAV SLR EWKW+SA+VG
Sbjct: 181 FLSHSTILGFMGGTAVIICLQQLKGIFGLTHFTSKTDVYSVLHAVFSLRKEWKWESALVG 240

Query: 241 VIFLIFLQFTRFLRNRNPKLFWVSAMAPMVTVIIGCLFAYFIKGEKHGILTAVWDVGIDW 300
           V+FL+FLQFTR+LRNR PKLFWVSAMAPMVTVI+GCLFAYFIKG +HGILT         
Sbjct: 241 VVFLLFLQFTRYLRNRKPKLFWVSAMAPMVTVIVGCLFAYFIKGSQHGILT--------- 300

Query: 301 VGMESHVIPMFGAPDLSGVGMELWTCGFQPNSHILVGHLSKGINPISIKFLNFDSKYISA 360
                                              VGHLSKGINPISI FLNFDSKY+SA
Sbjct: 301 -----------------------------------VGHLSKGINPISIHFLNFDSKYLSA 360

Query: 361 VLQAGLITGMVALAEGIAIGRSFAIMKNEQIDGNKEMVAFGLMNIVGSFTSCYLTTGPFS 420
           V+Q GLITG++ALAEGIAIGRSFAI+KNEQIDGNKEM+AFGLMNIVGSFTSCYLTTGPFS
Sbjct: 361 VVQTGLITGLIALAEGIAIGRSFAIIKNEQIDGNKEMIAFGLMNIVGSFTSCYLTTGPFS 420

Query: 421 KTAVNFNAGCRTAMSNIVMAVFMALTLLFLAPLFSYTPLVALSAIIMSAMLGLIKYEEMY 480
           KTAVNFNAGCRTAMSNIVMA+FMALTLLFLAP+FSYTPLVALSAIIMSAMLGLIKYEEMY
Sbjct: 421 KTAVNFNAGCRTAMSNIVMAIFMALTLLFLAPVFSYTPLVALSAIIMSAMLGLIKYEEMY 480

Query: 481 HIFIVDKFDFCICMAAFLGVALLSMDVGIMLSVGLALLRALLYMARPATCKLGKIPNSAL 540
           H+  VDKFDFCICMAAFLGVA LSMD+GIMLSVGLALLRALLYMARPATCKLGKIPNS L
Sbjct: 481 HLLKVDKFDFCICMAAFLGVAFLSMDIGIMLSVGLALLRALLYMARPATCKLGKIPNSNL 540

Query: 541 YRDMEQYPDSNRVPGIIVLQLGSPVYYANSNYIRERIFRWIRDEQAMFGSIDGSVQHVLL 600
           YRD+EQYP++ R  GIIVLQLGSP+YYANSNYI ERIFRW+RDEQ  F   DG V+HVLL
Sbjct: 541 YRDVEQYPNATRNHGIIVLQLGSPIYYANSNYITERIFRWVRDEQGNFE--DGPVEHVLL 600

Query: 601 ELSGVTSIDMTGIETLVEIRRSLQAMGIQMGIVNPRIVVMEKMIASEFTDTIGKENIYLS 660
           ELSGVTSIDMTG+ETL EIRRSLQA GIQMGIVNPRIVVMEKMIAS+FTDTIGKENIYLS
Sbjct: 601 ELSGVTSIDMTGLETLTEIRRSLQANGIQMGIVNPRIVVMEKMIASKFTDTIGKENIYLS 648

Query: 661 VDDGVERCRDTVVKPKKTDASERSSDVTTMEQRV 694
           VD+GVERCRD V K K+T+    SS++ TMEQ+V
Sbjct: 661 VDEGVERCRDLVPKLKQTETGAGSSNMRTMEQQV 648

BLAST of Spg022559 vs. NCBI nr
Match: XP_031737105.1 (LOW QUALITY PROTEIN: probable sulfate transporter 3.5 [Cucumis sativus])

HSP 1 Score: 1071.6 bits (2770), Expect = 2.7e-309
Identity = 560/694 (80.69%), Postives = 605/694 (87.18%), Query Frame = 0

Query: 1   MGSFKSDQKVSFADPRPFGKKLKSDLKETFFPDDPFRQFQDDR-ALHRVKKGVQYFIPIL 60
           MGS K+ + V+F+ PRPFGKKLKSDLKETFFPDDPF+QF+D+  A+ RVKKG QYFIPIL
Sbjct: 1   MGSLKNGENVNFSAPRPFGKKLKSDLKETFFPDDPFKQFRDESGAMDRVKKGFQYFIPIL 60

Query: 61  DWLPKYNFNMFKYDLLAGITITSLAIPQGISYAKLGSLPPIIGLYSSFVPPLVYAVFGSS 120
            WLPKYN NMFKYDLLAGITITSLAIPQGISYAKLG LPP IGLYSSFVPPLVYAVFGSS
Sbjct: 61  QWLPKYNLNMFKYDLLAGITITSLAIPQGISYAKLGILPPXIGLYSSFVPPLVYAVFGSS 120

Query: 121 KHLAVGTVAACSLLIADIIGAVASPEKEPTLYLHLVYTATFITGVFQAALGFLRLGILVD 180
           KHLAVGTVAACSLLI++ IGAVASPE+EPTLYLHLV+TATF+TGV QA LGFLRLGILVD
Sbjct: 121 KHLAVGTVAACSLLISETIGAVASPEEEPTLYLHLVFTATFVTGVMQAVLGFLRLGILVD 180

Query: 181 FLSHSTILGFMGGTAVIICLQQLKGIFGLTHFTSKTDVVSVLHAVISLRYEWKWQSAVVG 240
           FLSHSTILGFMGGTAVIICLQQLKGIFGLTHFTSKTDV SVLHAV SLR EWKW+SA+VG
Sbjct: 181 FLSHSTILGFMGGTAVIICLQQLKGIFGLTHFTSKTDVYSVLHAVFSLRKEWKWESALVG 240

Query: 241 VIFLIFLQFTRFLRNRNPKLFWVSAMAPMVTVIIGCLFAYFIKGEKHGILTAVWDVGIDW 300
           V+FL+FLQFTR+LRNR PKLFWVSAMAPMVTVI+GCLFAYFIKG +HGILT         
Sbjct: 241 VVFLLFLQFTRYLRNRKPKLFWVSAMAPMVTVIVGCLFAYFIKGSQHGILT--------- 300

Query: 301 VGMESHVIPMFGAPDLSGVGMELWTCGFQPNSHILVGHLSKGINPISIKFLNFDSKYISA 360
                                              VGHLSKGINPISI FLNFDSKY+SA
Sbjct: 301 -----------------------------------VGHLSKGINPISIHFLNFDSKYLSA 360

Query: 361 VLQAGLITGMVALAEGIAIGRSFAIMKNEQIDGNKEMVAFGLMNIVGSFTSCYLTTGPFS 420
           V+Q GLITG++ALAEGIAIGRSFAI+KNEQIDGNKEM+AFGLMNIVGSFTSCYLTTGPFS
Sbjct: 361 VVQTGLITGLIALAEGIAIGRSFAIIKNEQIDGNKEMIAFGLMNIVGSFTSCYLTTGPFS 420

Query: 421 KTAVNFNAGCRTAMSNIVMAVFMALTLLFLAPLFSYTPLVALSAIIMSAMLGLIKYEEMY 480
           KTAVNFNAGCRTAMSNIVMA+FMALTLLFLAP+FSYTPLVALSAIIMSAMLGLIKYEEMY
Sbjct: 421 KTAVNFNAGCRTAMSNIVMAIFMALTLLFLAPVFSYTPLVALSAIIMSAMLGLIKYEEMY 480

Query: 481 HIFIVDKFDFCICMAAFLGVALLSMDVGIMLSVGLALLRALLYMARPATCKLGKIPNSAL 540
           H+  VDKFDFCICMAAFLGVA LSMD+GIMLSVGLALLRALLYMARPATCKLGKIPNS L
Sbjct: 481 HLLKVDKFDFCICMAAFLGVAFLSMDIGIMLSVGLALLRALLYMARPATCKLGKIPNSNL 540

Query: 541 YRDMEQYPDSNRVPGIIVLQLGSPVYYANSNYIRERIFRWIRDEQAMFGSIDGSVQHVLL 600
           YRD+EQYP++ R  GIIVLQLGSP+YYANSNYI ERIFRW+RDEQ  F   DG V+HVLL
Sbjct: 541 YRDVEQYPNATRNHGIIVLQLGSPIYYANSNYITERIFRWVRDEQGNFE--DGPVEHVLL 600

Query: 601 ELSGVTSIDMTGIETLVEIRRSLQAMGIQMGIVNPRIVVMEKMIASEFTDTIGKENIYLS 660
           ELSGVTSIDMTG+ETL+EIRRSLQA GIQMGIVNPRIVVMEKMIAS+FTDTIGKENIYLS
Sbjct: 601 ELSGVTSIDMTGLETLIEIRRSLQANGIQMGIVNPRIVVMEKMIASKFTDTIGKENIYLS 648

Query: 661 VDDGVERCRDTVVKPKKTDASERSSDVTTMEQRV 694
           VD+GVERCRD V K K+T+    SS++ TMEQ+V
Sbjct: 661 VDEGVERCRDLVPKLKQTETGAGSSNMRTMEQQV 648

BLAST of Spg022559 vs. NCBI nr
Match: XP_038879869.1 (probable sulfate transporter 3.5 [Benincasa hispida])

HSP 1 Score: 1070.8 bits (2768), Expect = 4.8e-309
Identity = 561/694 (80.84%), Postives = 608/694 (87.61%), Query Frame = 0

Query: 1   MGSFKSDQKVSFADPRPFGKKLKSDLKETFFPDDPFRQFQDDRA-LHRVKKGVQYFIPIL 60
           MGS K+   VSF  PRPFG++LKSDLKETFFPDDPF QF+++R  + RVKKG QYFIPIL
Sbjct: 1   MGSLKNVPNVSFNAPRPFGQRLKSDLKETFFPDDPFMQFREERGPMQRVKKGFQYFIPIL 60

Query: 61  DWLPKYNFNMFKYDLLAGITITSLAIPQGISYAKLGSLPPIIGLYSSFVPPLVYAVFGSS 120
           +WLPKYN NMFKYDLLAGITITSLAIPQGISYAKLG +PPI+GLYSSFVPPL+YAVFGSS
Sbjct: 61  EWLPKYNLNMFKYDLLAGITITSLAIPQGISYAKLGVIPPIVGLYSSFVPPLIYAVFGSS 120

Query: 121 KHLAVGTVAACSLLIADIIGAVASPEKEPTLYLHLVYTATFITGVFQAALGFLRLGILVD 180
            HLAVGTVAACSLLI++IIG VASPE+EPTLYLHLV+TATFITG+ Q+ALGFLRLGILVD
Sbjct: 121 NHLAVGTVAACSLLISEIIGGVASPEEEPTLYLHLVFTATFITGIMQSALGFLRLGILVD 180

Query: 181 FLSHSTILGFMGGTAVIICLQQLKGIFGLTHFTSKTDVVSVLHAVISLRYEWKWQSAVVG 240
           FLSHSTILGFMGGTAVIICLQQLKGIFGLT FTSKTDV SVLHAV SLR EW+WQSAVVG
Sbjct: 181 FLSHSTILGFMGGTAVIICLQQLKGIFGLTRFTSKTDVYSVLHAVFSLRKEWRWQSAVVG 240

Query: 241 VIFLIFLQFTRFLRNRNPKLFWVSAMAPMVTVIIGCLFAYFIKGEKHGILTAVWDVGIDW 300
           V+FL+FLQFTR+LRNRNPKLFWVSAMAPMVTVIIGC+FAYFIKG ++GILT         
Sbjct: 241 VVFLVFLQFTRYLRNRNPKLFWVSAMAPMVTVIIGCVFAYFIKGSQNGILT--------- 300

Query: 301 VGMESHVIPMFGAPDLSGVGMELWTCGFQPNSHILVGHLSKGINPISIKFLNFDSKYISA 360
                                              VGHLSKGINP+SI FLNFDSKY+SA
Sbjct: 301 -----------------------------------VGHLSKGINPLSIHFLNFDSKYLSA 360

Query: 361 VLQAGLITGMVALAEGIAIGRSFAIMKNEQIDGNKEMVAFGLMNIVGSFTSCYLTTGPFS 420
           V+QAGLITG++ALAEGIAIGRSFAIMKNEQIDGNKEM+AFGLMNIVGSFTSCYLTTGPFS
Sbjct: 361 VVQAGLITGLIALAEGIAIGRSFAIMKNEQIDGNKEMIAFGLMNIVGSFTSCYLTTGPFS 420

Query: 421 KTAVNFNAGCRTAMSNIVMAVFMALTLLFLAPLFSYTPLVALSAIIMSAMLGLIKYEEMY 480
           KTAVN+NAGCRT MSNIVMA+ MALTLLFLAPLFSYTPLVALSAIIMSAMLGLIKYEEMY
Sbjct: 421 KTAVNYNAGCRTPMSNIVMAICMALTLLFLAPLFSYTPLVALSAIIMSAMLGLIKYEEMY 480

Query: 481 HIFIVDKFDFCICMAAFLGVALLSMDVGIMLSVGLALLRALLYMARPATCKLGKIPNSAL 540
           H+  VDKFDFCICMAAFLGVA LSMDVGIMLSVGLALLRALLYMARPATCKLGKIPNS+L
Sbjct: 481 HLLKVDKFDFCICMAAFLGVAFLSMDVGIMLSVGLALLRALLYMARPATCKLGKIPNSSL 540

Query: 541 YRDMEQYPDSNRVPGIIVLQLGSPVYYANSNYIRERIFRWIRDEQAMFGSIDGSVQHVLL 600
           YRD+EQYP++ RV GI+V+QLGSPVYYAN NYIR+RIFRW+RDEQ+   S  GSV+HVLL
Sbjct: 541 YRDVEQYPNATRVHGIMVIQLGSPVYYANCNYIRDRIFRWVRDEQS--DSECGSVEHVLL 600

Query: 601 ELSGVTSIDMTGIETLVEIRRSLQAMGIQMGIVNPRIVVMEKMIASEFTDTIGKENIYLS 660
           ELSGVTSIDMTGIETLVEIRRSLQA GIQMGIVNPRIVVMEKMIASEFTDTIGKENIYLS
Sbjct: 601 ELSGVTSIDMTGIETLVEIRRSLQANGIQMGIVNPRIVVMEKMIASEFTDTIGKENIYLS 648

Query: 661 VDDGVERCRDTVVKPKKTDASERSSDVTTMEQRV 694
           VD+GVERCRD + K K+T  S +SSDVTTMEQRV
Sbjct: 661 VDEGVERCRDLIPKLKQTATSSQSSDVTTMEQRV 648

BLAST of Spg022559 vs. NCBI nr
Match: TYK00091.1 (putative sulfate transporter 3.5 [Cucumis melo var. makuwa])

HSP 1 Score: 1070.5 bits (2767), Expect = 6.4e-309
Identity = 565/700 (80.71%), Postives = 604/700 (86.29%), Query Frame = 0

Query: 1   MGSFKSDQKVSFADPRPFGKKLKSDLKETFFPDDPFRQFQDDR-ALHRVKKGVQYFIPIL 60
           MGS K+ Q V+F+ PRPFGK+LKSDLKETFFPDDPFRQF+D+R A+HR KKG QYFIPIL
Sbjct: 1   MGSLKNGQNVNFSAPRPFGKRLKSDLKETFFPDDPFRQFRDERGAMHRAKKGFQYFIPIL 60

Query: 61  DWLPKYNFNMFKYDLLAGITITSLAIPQGISYAKLGSLPPIIGLYSSFVPPLVYAVFGSS 120
           DWLPKYN NMFKYDLLAGITITSLAIPQGISYAKLG LPPIIGLYSSFVPPL+YAVFGSS
Sbjct: 61  DWLPKYNLNMFKYDLLAGITITSLAIPQGISYAKLGVLPPIIGLYSSFVPPLIYAVFGSS 120

Query: 121 KHLAVGTVAACSLLIADIIGAVASPEKEPTLYLHLVYTATFITGVFQAALGFLRLGILVD 180
           KHLAVGTVAACSLLIA+ IG VASPE+EPTLYLHLV+TATF+TG+ QA LGFLRLGILVD
Sbjct: 121 KHLAVGTVAACSLLIAETIGGVASPEEEPTLYLHLVFTATFVTGIMQAVLGFLRLGILVD 180

Query: 181 FLSHSTILGFMGGTAVIICLQQLKGIFGLTHFTSKTDVVSVLHAVISLRYEWKWQSAVVG 240
           FLSHSTILGFMGGTAVIICLQQLKGIFGLTHFTSKTDV SVLHAV SLR EWKW+SAVVG
Sbjct: 181 FLSHSTILGFMGGTAVIICLQQLKGIFGLTHFTSKTDVYSVLHAVFSLRKEWKWESAVVG 240

Query: 241 VIFLIFLQFTRFLRNRNPKLFWVSAMAPMVTVIIGCLFAYFIKGEKHGILTAVWDVGIDW 300
           VIFL+FLQFTR+LRNRNPKLFWVSAMAPMVTVIIGCLFAYFIKG +HGILT         
Sbjct: 241 VIFLLFLQFTRYLRNRNPKLFWVSAMAPMVTVIIGCLFAYFIKGSQHGILT--------- 300

Query: 301 VGMESHVIPMFGAPDLSGVGMELWTCGFQPNSHILVGHLSKGINPISIKFLNFDSKYISA 360
                                              VGHLSKGINPISI FLNFDSKY+SA
Sbjct: 301 -----------------------------------VGHLSKGINPISIHFLNFDSKYLSA 360

Query: 361 VLQAGLITGMVALAEGIAIGRSFAIMKNEQIDGNKEMVAFGLMNIVGSFTSCYLTTGPFS 420
           V+Q GLITG+VALAEGIAIGRSFAIMKNEQIDGNKEM+AFGLMNIVGSFTSCYLTTGPFS
Sbjct: 361 VVQTGLITGLVALAEGIAIGRSFAIMKNEQIDGNKEMIAFGLMNIVGSFTSCYLTTGPFS 420

Query: 421 KTAVNFNAGCRTAMSNIVMAVFMALTLLFLAPLFSYTPLVALSAIIMSAMLGLIKYEEMY 480
           KTAVNFNAGCRTAMSN+VMA+FMALTL+FLAP+FSYTPLVALSAIIMSAMLGLIKYEEMY
Sbjct: 421 KTAVNFNAGCRTAMSNVVMAIFMALTLIFLAPVFSYTPLVALSAIIMSAMLGLIKYEEMY 480

Query: 481 HIFIVDKFDFCICMAAFLGVALLSMDVGIMLSVGLALLRALLYMARPATCKLGKIPNSAL 540
           H+  VDKFDFCICMAAFLGVA LSMDVGIMLSVGLALLRALLYMARPATCKLGKIPNS+L
Sbjct: 481 HLLKVDKFDFCICMAAFLGVAFLSMDVGIMLSVGLALLRALLYMARPATCKLGKIPNSSL 540

Query: 541 YRDMEQYPDSNRVPGIIVLQLGSPVYYANSNYIRERIFRWIRDEQAMFGSIDGSVQHVLL 600
           YRD+EQYP++ R  GIIVLQLGSP+YYANSNYIRERIFRW+RDEQ    S DG V+HVLL
Sbjct: 541 YRDVEQYPNATRTHGIIVLQLGSPIYYANSNYIRERIFRWVRDEQG--DSKDGCVEHVLL 600

Query: 601 ELSGVTSIDMTGIETLVEIRRSLQAMGIQMGIVNPRIVVMEKMIASEFTDTIGKENIYLS 660
           ELSGVTSIDMTGIETL+EIRRSLQA GIQMGIVNPRIVVMEKMIASEFT TIGKENIYLS
Sbjct: 601 ELSGVTSIDMTGIETLIEIRRSLQANGIQMGIVNPRIVVMEKMIASEFTKTIGKENIYLS 654

Query: 661 VDDGVER--CR----DTVVKPKKTDASERSSDVTTMEQRV 694
           VD+GVER  CR      V KP  +    RSS + T+ Q +
Sbjct: 661 VDEGVERSVCRKSLLSNVAKPTPSSKLYRSSSLPTLCQTI 654

BLAST of Spg022559 vs. ExPASy Swiss-Prot
Match: Q94LW6 (Probable sulfate transporter 3.5 OS=Arabidopsis thaliana OX=3702 GN=SULTR3;5 PE=2 SV=1)

HSP 1 Score: 820.1 bits (2117), Expect = 1.9e-236
Identity = 422/669 (63.08%), Postives = 512/669 (76.53%), Query Frame = 0

Query: 10  VSFADPRPFGKKLKSDLKETFFPDDPFRQF-QDDRALHRVKKGVQYFIPILDWLPKYNFN 69
           V+F+ PR FG K KS  KETFFPDDPF+   Q+   L + KK ++YF+PI +WLPKY+  
Sbjct: 17  VNFSTPRGFGSKFKSKCKETFFPDDPFKPISQEPNRLLKTKKLLEYFVPIFEWLPKYDMQ 76

Query: 70  MFKYDLLAGITITSLAIPQGISYAKLGSLPPIIGLYSSFVPPLVYAVFGSSKHLAVGTVA 129
             KYD+LAGITITSLA+PQGISYAKL S+PPIIGLYSSFVPP VYAVFGSS +LAVGTVA
Sbjct: 77  KLKYDVLAGITITSLAVPQGISYAKLASIPPIIGLYSSFVPPFVYAVFGSSNNLAVGTVA 136

Query: 130 ACSLLIADIIGAVASPEKEPTLYLHLVYTATFITGVFQAALGFLRLGILVDFLSHSTILG 189
           ACSLLIA+  G     + EP LYLHL++TAT ITG+FQ A+GFLRLGILVDFLSHSTI G
Sbjct: 137 ACSLLIAETFGE-EMIKNEPELYLHLIFTATLITGLFQFAMGFLRLGILVDFLSHSTITG 196

Query: 190 FMGGTAVIICLQQLKGIFGLTHFTSKTDVVSVLHAVISLRYEWKWQSAVVGVIFLIFLQF 249
           FMGGTA+II LQQLKGIFGL HFT KTDVVSVLH+++  R EWKWQS + GV FL+FLQ 
Sbjct: 197 FMGGTAIIILLQQLKGIFGLVHFTHKTDVVSVLHSILDNRAEWKWQSTLAGVCFLVFLQS 256

Query: 250 TRFLRNRNPKLFWVSAMAPMVTVIIGCLFAYFIKGEKHGILTAVWDVGIDWVGMESHVIP 309
           TR+++ R PKLFWVSAM PMV V++GC+ AY +KG  HGI T                  
Sbjct: 257 TRYIKQRYPKLFWVSAMGPMVVVVVGCVVAYLVKGTAHGIAT------------------ 316

Query: 310 MFGAPDLSGVGMELWTCGFQPNSHILVGHLSKGINPISIKFLNFDSKYISAVLQAGLITG 369
                                     VG L KG+NP SI+ LNFDSKY+  V +AG++TG
Sbjct: 317 --------------------------VGPLKKGLNPPSIQLLNFDSKYLGMVFKAGIVTG 376

Query: 370 MVALAEGIAIGRSFAIMKNEQIDGNKEMVAFGLMNIVGSFTSCYLTTGPFSKTAVNFNAG 429
           ++ALAEGIAIGRSFA+MKNEQ DGNKEM+AFGLMN++GSFTSCYLTTGPFSKTAVN+NAG
Sbjct: 377 LIALAEGIAIGRSFAVMKNEQTDGNKEMIAFGLMNVIGSFTSCYLTTGPFSKTAVNYNAG 436

Query: 430 CRTAMSNIVMAVFMALTLLFLAPLFSYTPLVALSAIIMSAMLGLIKYEEMYHIFIVDKFD 489
            +T MSN+VM V M L LLFLAPLFSYTPLV LSAIIMSAMLGLI YEEMYH+F VDKFD
Sbjct: 437 TKTPMSNVVMGVCMMLVLLFLAPLFSYTPLVGLSAIIMSAMLGLINYEEMYHLFKVDKFD 496

Query: 490 FCICMAAFLGVALLSMDVGIMLSVGLALLRALLYMARPATCKLGKIPNSALYRDMEQYPD 549
           F +CM+AF GV+ LSMD G+++SVG +++RALLY+ARP+TCKLG+IPNS ++RD+EQYP 
Sbjct: 497 FLVCMSAFFGVSFLSMDYGLIISVGFSIVRALLYVARPSTCKLGRIPNSVMFRDIEQYPA 556

Query: 550 SNRVPGIIVLQLGSPVYYANSNYIRERIFRWIRDEQAMFGSIDGSVQHVLLELSGVTSID 609
           S  + G I+LQLGSPV++ANS Y+RERI RWIRDE         +++ +LL+LSGV++ID
Sbjct: 557 SEEMLGYIILQLGSPVFFANSTYVRERILRWIRDEPE-------AIEFLLLDLSGVSTID 616

Query: 610 MTGIETLVEIRRSLQAMGIQMGIVNPRIVVMEKMIASEFTDTIGKENIYLSVDDGVERCR 669
           MTG+ETL+EI+R L +  I+M I+NPR  V+EKM+ S F + IGKE ++LS+DD V+ CR
Sbjct: 617 MTGMETLLEIQRILGSKNIKMVIINPRFEVLEKMMLSHFVEKIGKEYMFLSIDDAVQACR 633

Query: 670 D--TVVKPK 676
              T  KP+
Sbjct: 677 FNLTTTKPE 633

BLAST of Spg022559 vs. ExPASy Swiss-Prot
Match: Q9SV13 (Sulfate transporter 3.1 OS=Arabidopsis thaliana OX=3702 GN=SULTR3;1 PE=2 SV=1)

HSP 1 Score: 681.4 bits (1757), Expect = 1.1e-194
Identity = 344/660 (52.12%), Postives = 468/660 (70.91%), Query Frame = 0

Query: 10  VSFADPRPFGKKLKSDLKETFFPDDPFRQFQDDRALHRVKKGVQYFIPILDWLPKYNFNM 69
           V    P+PF K L+  +KET FPDDPFRQF++  A  +   G++YF+PI +W P+YN   
Sbjct: 22  VEAPQPQPFLKSLQYSVKETLFPDDPFRQFKNQNASRKFVLGLKYFLPIFEWAPRYNLKF 81

Query: 70  FKYDLLAGITITSLAIPQGISYAKLGSLPPIIGLYSSFVPPLVYAVFGSSKHLAVGTVAA 129
           FK DL+AGITI SLAIPQGISYAKL +LPPI+GLYSSFVPPLVYAV GSS+ LAVGTVA 
Sbjct: 82  FKSDLIAGITIASLAIPQGISYAKLANLPPILGLYSSFVPPLVYAVLGSSRDLAVGTVAV 141

Query: 130 CSLLIADIIGAVASPEKEPTLYLHLVYTATFITGVFQAALGFLRLGILVDFLSHSTILGF 189
            SLL   ++      EK+P LYLHL +TATF  GV +A+LG  RLG +VDFLSH+TI+GF
Sbjct: 142 ASLLTGAMLSKEVDAEKDPKLYLHLAFTATFFAGVLEASLGIFRLGFIVDFLSHATIVGF 201

Query: 190 MGGTAVIICLQQLKGIFGLTHFTSKTDVVSVLHAVISLRYEWKWQSAVVGVIFLIFLQFT 249
           MGG A ++ LQQLKGIFGL HFT  TDV+SV+ +V S  +EW+W+S V+G  FL FL  T
Sbjct: 202 MGGAATVVSLQQLKGIFGLKHFTDSTDVISVMRSVFSQTHEWRWESGVLGCGFLFFLLST 261

Query: 250 RFLRNRNPKLFWVSAMAPMVTVIIGCLFAYFIKGEKHGILTAVWDVGIDWVGMESHVIPM 309
           R+   + PK FWV+AMAP+ +VI+G L  YF   E+HG+                     
Sbjct: 262 RYFSIKKPKFFWVAAMAPLTSVILGSLLVYFTHAERHGVQ-------------------- 321

Query: 310 FGAPDLSGVGMELWTCGFQPNSHILVGHLSKGINPISIKFLNFDSKYISAVLQAGLITGM 369
                                   ++G L KG+NP+S   L F S Y+S  ++ GLITG+
Sbjct: 322 ------------------------VIGDLKKGLNPLSGSDLIFTSPYMSTAVKTGLITGI 381

Query: 370 VALAEGIAIGRSFAIMKNEQIDGNKEMVAFGLMNIVGSFTSCYLTTGPFSKTAVNFNAGC 429
           +ALAEG+A+GRSFA+ KN  IDGNKEM+AFG+MNIVGSFTSCYLTTGPFS++AVN+NAGC
Sbjct: 382 IALAEGVAVGRSFAMFKNYNIDGNKEMIAFGMMNIVGSFTSCYLTTGPFSRSAVNYNAGC 441

Query: 430 RTAMSNIVMAVFMALTLLFLAPLFSYTPLVALSAIIMSAMLGLIKYEEMYHIFIVDKFDF 489
           +TAMSNIVMA+ +  TLLFL PLF YTPLV LSAII+SAMLGLI Y+   H++ VDKFDF
Sbjct: 442 KTAMSNIVMAIAVMFTLLFLTPLFHYTPLVVLSAIIISAMLGLIDYQAAIHLWKVDKFDF 501

Query: 490 CICMAAFLGVALLSMDVGIMLSVGLALLRALLYMARPATCKLGKIPNSALYRDMEQYPDS 549
            +CM+A++GV   S+++G++++V +++ R LL+++RP T   G IPNS +YR+ EQYP S
Sbjct: 502 LVCMSAYVGVVFGSVEIGLVVAVAISIARLLLFVSRPKTAVKGNIPNSMIYRNTEQYPSS 561

Query: 550 NRVPGIIVLQLGSPVYYANSNYIRERIFRWI-RDEQAMFGSIDGSVQHVLLELSGVTSID 609
             VPGI++L++ +P+Y+AN++Y+RERI RWI  +E+ +  S + S+Q+++L++S V +ID
Sbjct: 562 RTVPGILILEIDAPIYFANASYLRERIIRWIDEEEERVKQSGESSLQYIILDMSAVGNID 621

Query: 610 MTGIETLVEIRRSLQAMGIQMGIVNPRIVVMEKMIASEFT-DTIGKENIYLSVDDGVERC 668
            +GI  +VEI++ +    +++ + NP+  V++K+  S+F  D +GKE ++L+V + VE C
Sbjct: 622 TSGISMMVEIKKVIDRRALKLVLSNPKGEVVKKLTRSKFIGDHLGKEWMFLTVGEAVEAC 637

BLAST of Spg022559 vs. ExPASy Swiss-Prot
Match: O04289 (Sulfate transporter 3.2 OS=Arabidopsis thaliana OX=3702 GN=SULTR3;2 PE=2 SV=1)

HSP 1 Score: 625.2 bits (1611), Expect = 9.1e-178
Identity = 325/683 (47.58%), Postives = 463/683 (67.79%), Query Frame = 0

Query: 1   MGSFKSDQ--KVSFADPRPFGKKLKSDLKETFFPDDPFRQFQDD-RALHRVKKGVQYFIP 60
           M S ++ Q  +V    P+PF K LK+ L E  F DDPFR+ +++ +   +++ G+++  P
Sbjct: 1   MSSKRASQYHQVEIPPPQPFLKSLKNTLNEILFADDPFRRIRNESKTSKKIELGLRHVFP 60

Query: 61  ILDWLPKYNFNMFKYDLLAGITITSLAIPQGISYAKLGSLPPIIGLYSSFVPPLVYAVFG 120
           IL+W   Y+    K D+++GITI SLAIPQGISYA+L +LPPI+GLYSS VPPLVYA+ G
Sbjct: 61  ILEWARGYSLEYLKSDVISGITIASLAIPQGISYAQLANLPPILGLYSSLVPPLVYAIMG 120

Query: 121 SSKHLAVGTVAACSLLIADIIGAVASPEKEPTLYLHLVYTATFITGVFQAALGFLRLGIL 180
           SS+ LAVGTVA  SLL A ++G   +    P LYLHL +TATF  G+ Q  LG LRLG +
Sbjct: 121 SSRDLAVGTVAVASLLTAAMLGKEVNAVVNPKLYLHLAFTATFFAGLMQTCLGLLRLGFV 180

Query: 181 VDFLSHSTILGFMGGTAVIICLQQLKGIFGLTHFTSKTDVVSVLHAVISLRYEWKWQSAV 240
           V+ LSH+ I+GFMGG A ++CLQQLKG+ GL HFT  TD+V+VL ++ S  + W+W+S V
Sbjct: 181 VEILSHAAIVGFMGGAATVVCLQQLKGLLGLHHFTHSTDIVTVLRSIFSQSHMWRWESGV 240

Query: 241 VGVIFLIFLQFTRFLRNRNPKLFWVSAMAPMVTVIIGCLFAYFIKGEKHGILTAVWDVGI 300
           +G  FLIFL  T+++  + PKLFW+SAM+P+V+VI G +F YF+  + HGI         
Sbjct: 241 LGCCFLIFLLTTKYISKKRPKLFWISAMSPLVSVIFGTIFLYFLHDQFHGIQ-------- 300

Query: 301 DWVGMESHVIPMFGAPDLSGVGMELWTCGFQPNSHILVGHLSKGINPISIKFLNFDSKYI 360
                                                +G L KGINP SI  L F   Y+
Sbjct: 301 ------------------------------------FIGELKKGINPPSITHLVFTPPYV 360

Query: 361 SAVLQAGLITGMVALAEGIAIGRSFAIMKNEQIDGNKEMVAFGLMNIVGSFTSCYLTTGP 420
              L+ G+ITG++ALAEGIA+GRSFA+ KN  IDGNKEM+AFG+MNI+GSF+SCYLTTGP
Sbjct: 361 MLALKVGIITGVIALAEGIAVGRSFAMYKNYNIDGNKEMIAFGMMNILGSFSSCYLTTGP 420

Query: 421 FSKTAVNFNAGCRTAMSNIVMAVFMALTLLFLAPLFSYTPLVALSAIIMSAMLGLIKYEE 480
           FS++AVN+NAGC+TA+SN+VMAV +A+TLLFL PLF YTPLV LS+II++AMLGL+ YE 
Sbjct: 421 FSRSAVNYNAGCKTALSNVVMAVAVAVTLLFLTPLFFYTPLVVLSSIIIAAMLGLVDYEA 480

Query: 481 MYHIFIVDKFDFCICMAAFLGVALLSMDVGIMLSVGLALLRALLYMARPATCKLGKIPNS 540
             H++ +DKFDF +C++A+LGV   ++++G++LSVG++++R +L++ RP    +G I NS
Sbjct: 481 AIHLWKLDKFDFFVCLSAYLGVVFGTIEIGLILSVGISVMRLVLFVGRPKIYVMGNIQNS 540

Query: 541 ALYRDMEQYPDSNRVPGIIVLQLGSPVYYANSNYIRERIFRWI-RDEQAMFGSIDGSVQH 600
            +YR++E YP +     +++L +  P+Y+ANS Y+R+RI RWI  +E  +  S D S+Q+
Sbjct: 541 EIYRNIEHYPQAITRSSLLILHIDGPIYFANSTYLRDRIGRWIDEEEDKLRTSGDISLQY 600

Query: 601 VLLELSGVTSIDMTGIETLVEIRRSLQAMGIQMGIVNPRIVVMEKMIASEFTDTIGKENI 660
           ++L++S V +ID +GI  L E+ + L    +++ I NP   VM+K+  S F ++IGKE I
Sbjct: 601 IVLDMSAVGNIDTSGISMLEELNKILGRRELKLVIANPGAEVMKKLSKSTFIESIGKERI 638

Query: 661 YLSVDDGVERCRDTVVKPKKTDA 680
           YL+V + V  C D ++   K D+
Sbjct: 661 YLTVAEAVAAC-DFMLHTAKPDS 638

BLAST of Spg022559 vs. ExPASy Swiss-Prot
Match: Q9SAY1 (Sulfate transporter 1.1 OS=Arabidopsis thaliana OX=3702 GN=SULTR1;1 PE=1 SV=2)

HSP 1 Score: 580.1 bits (1494), Expect = 3.3e-164
Identity = 304/656 (46.34%), Postives = 427/656 (65.09%), Query Frame = 0

Query: 20  KKLKSDLKETFFPDDPFRQFQDDRALHRVKKGVQYFIPILDWLPKYNFNMFKYDLLAGIT 79
           K +KS ++ETFF D P R F+      +   G+Q   PI+ W  +Y    F+ DL+AG+T
Sbjct: 35  KDIKSVVEETFFHDAPLRDFKGQTPAKKALLGIQAVFPIIGWAREYTLRKFRGDLIAGLT 94

Query: 80  ITSLAIPQGISYAKLGSLPPIIGLYSSFVPPLVYAVFGSSKHLAVGTVAACSLLIADIIG 139
           I SL IPQ I YAKL ++ P  GLYSSFVPPL+YA  GSS+ +A+G VA  SLL+  +  
Sbjct: 95  IASLCIPQDIGYAKLANVDPKYGLYSSFVPPLIYAGMGSSRDIAIGPVAVVSLLVGTLCQ 154

Query: 140 AVASPEKEPTLYLHLVYTATFITGVFQAALGFLRLGILVDFLSHSTILGFMGGTAVIICL 199
           AV  P+K P  YL LV+TATF  G+FQA LGFLRLG L+DFLSH+ ++GFMGG A+ I L
Sbjct: 155 AVIDPKKNPEDYLRLVFTATFFAGIFQAGLGFLRLGFLIDFLSHAAVVGFMGGAAITIAL 214

Query: 200 QQLKGIFGLTHFTSKTDVVSVLHAVI-SLRYEWKWQSAVVGVIFLIFLQFTRFLRNRNPK 259
           QQLKG  G+  FT KTD+VSV+H+V  +  + W WQ+ V+G  FL FL  T+F+  RN K
Sbjct: 215 QQLKGFLGIKTFTKKTDIVSVMHSVFKNAEHGWNWQTIVIGASFLTFLLVTKFIGKRNRK 274

Query: 260 LFWVSAMAPMVTVIIGCLFAYFIKGEKHGILTAVWDVGIDWVGMESHVIPMFGAPDLSGV 319
           LFWV A+AP+++VII   F +  + +K G+                              
Sbjct: 275 LFWVPAIAPLISVIISTFFVFIFRADKQGVQ----------------------------- 334

Query: 320 GMELWTCGFQPNSHILVGHLSKGINPISIKFLNFDSKYISAVLQAGLITGMVALAEGIAI 379
                          +V H+ +GINPIS+  + F  KY +  ++ G I GMVAL E +AI
Sbjct: 335 ---------------IVKHIDQGINPISVHKIFFSGKYFTEGIRIGGIAGMVALTEAVAI 394

Query: 380 GRSFAIMKNEQIDGNKEMVAFGLMNIVGSFTSCYLTTGPFSKTAVNFNAGCRTAMSNIVM 439
            R+FA MK+ QIDGNKEM+A G MN+VGS TSCY+ TG FS++AVNF AG  TA+SNIVM
Sbjct: 395 ARTFAAMKDYQIDGNKEMIALGTMNVVGSMTSCYIATGSFSRSAVNFMAGVETAVSNIVM 454

Query: 440 AVFMALTLLFLAPLFSYTPLVALSAIIMSAMLGLIKYEEMYHIFIVDKFDFCICMAAFLG 499
           A+ +ALTL F+ PLF YTP   L+AII+SA+LGLI  +    I+ +DK DF  CM AFLG
Sbjct: 455 AIVVALTLEFITPLFKYTPNAILAAIIISAVLGLIDIDAAILIWRIDKLDFLACMGAFLG 514

Query: 500 VALLSMDVGIMLSVGLALLRALLYMARPATCKLGKIPNSALYRDMEQYPDSNRVPGIIVL 559
           V  +S+++G++++V ++  + LL + RP T  LGK+PNS +YR+  QYPD+ ++PGI+++
Sbjct: 515 VIFISVEIGLLIAVVISFAKILLQVTRPRTTVLGKLPNSNVYRNTLQYPDAAQIPGILII 574

Query: 560 QLGSPVYYANSNYIRERIFRWIRDEQAMFGSID-GSVQHVLLELSGVTSIDMTGIETLVE 619
           ++ S +Y++NSNY+RER  RW+R+EQ         +++ V++E+S VT ID +GI ++ E
Sbjct: 575 RVDSAIYFSNSNYVRERASRWVREEQENAKEYGMPAIRFVIIEMSPVTDIDTSGIHSIEE 634

Query: 620 IRRSLQAMGIQMGIVNPRIVVMEKMIASEFTDTIGKENIYLSVDDGVERCRDTVVK 674
           + +SL+   IQ+ + NP  VV+EK+ AS+F + IG++NI+L+V D V  C   V +
Sbjct: 635 LLKSLEKQEIQLILANPGPVVIEKLYASKFVEEIGEKNIFLTVGDAVAVCSTEVAE 646

BLAST of Spg022559 vs. ExPASy Swiss-Prot
Match: Q9FEP7 (Sulfate transporter 1.3 OS=Arabidopsis thaliana OX=3702 GN=SULTR1;3 PE=2 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 9.7e-164
Identity = 298/654 (45.57%), Postives = 423/654 (64.68%), Query Frame = 0

Query: 27  KETFFPDDPFRQFQDDRALHRVKKGVQYFIPILDWLPKYNFNMFKYDLLAGITITSLAIP 86
           KETFF DDP R F+D     ++  G+Q   P+++W  KYN  +F+ DL+AG+TI SL IP
Sbjct: 50  KETFFHDDPLRHFKDQSKSKKLMLGIQSVFPVIEWGRKYNLKLFRGDLIAGLTIASLCIP 109

Query: 87  QGISYAKLGSLPPIIGLYSSFVPPLVYAVFGSSKHLAVGTVAACSLLIADIIGAVASPEK 146
           Q I YAKL SL P  GLYSSFVPPLVYA  GSSK +A+G VA  SLL+  ++ A   P  
Sbjct: 110 QDIGYAKLASLDPKYGLYSSFVPPLVYACMGSSKDIAIGPVAVVSLLLGTLLRAEIDPNT 169

Query: 147 EPTLYLHLVYTATFITGVFQAALGFLRLGILVDFLSHSTILGFMGGTAVIICLQQLKGIF 206
            P  YL L +T+TF  GV QAALGF RLG L+DFLSH+ ++GFMGG A+ I LQQLKG  
Sbjct: 170 NPNEYLRLAFTSTFFAGVTQAALGFFRLGFLIDFLSHAAVVGFMGGAAITIALQQLKGFL 229

Query: 207 GLTHFTSKTDVVSVLHAVI-SLRYEWKWQSAVVGVIFLIFLQFTRFLRNRNPKLFWVSAM 266
           G+  FT KTD+++VL +VI S  + W WQ+ ++   FLIFL  ++F+  RN KLFW+ A+
Sbjct: 230 GINKFTKKTDIIAVLSSVISSAHHGWNWQTILISASFLIFLLISKFIGKRNKKLFWIPAI 289

Query: 267 APMVTVIIGCLFAYFIKGEKHGILTAVWDVGIDWVGMESHVIPMFGAPDLSGVGMELWTC 326
           AP+V+VII   F Y  + +K G+                                     
Sbjct: 290 APLVSVIISTFFVYITRADKKGVQ------------------------------------ 349

Query: 327 GFQPNSHILVGHLSKGINPISIKFLNFDSKYISAVLQAGLITGMVALAEGIAIGRSFAIM 386
                   +V HL KG+NP S++ + F   Y+    + G+++GMVAL E +AIGR+FA M
Sbjct: 350 --------IVKHLDKGLNPSSLRLIYFSGDYLLKGFRIGVVSGMVALTEAVAIGRTFAAM 409

Query: 387 KNEQIDGNKEMVAFGLMNIVGSFTSCYLTTGPFSKTAVNFNAGCRTAMSNIVMAVFMALT 446
           K+ QIDGNKEMVA G MN++GS TSCY++TG FS++AVNF AGC+TA+SNI+M++ + LT
Sbjct: 410 KDYQIDGNKEMVALGAMNVIGSMTSCYVSTGSFSRSAVNFMAGCQTAVSNIIMSIVVLLT 469

Query: 447 LLFLAPLFSYTPLVALSAIIMSAMLGLIKYEEMYHIFIVDKFDFCICMAAFLGVALLSMD 506
           LLFL PLF YTP   L+AII++A++ L+       IF +DK DF  CM AF GV  +S++
Sbjct: 470 LLFLTPLFKYTPNAILAAIIINAVIPLVDVNATILIFKIDKLDFVACMGAFFGVIFVSVE 529

Query: 507 VGIMLSVGLALLRALLYMARPATCKLGKIPNSALYRDMEQYPDSNRVPGIIVLQLGSPVY 566
           +G++++VG++  + LL + RP T  LGKIP +++YR++ QYP++ R+PG++ +++ S +Y
Sbjct: 530 IGLLIAVGISFAKILLQVTRPRTAILGKIPGTSVYRNINQYPEATRIPGVLTIRVDSAIY 589

Query: 567 YANSNYIRERIFRWIRDEQAMFGSID-GSVQHVLLELSGVTSIDMTGIETLVEIRRSLQA 626
           ++NSNY+RERI RW+ DE+ M  +     +Q +++E+S VT ID +GI  L ++ +SLQ 
Sbjct: 590 FSNSNYVRERIQRWLTDEEEMVEAARLPRIQFLIIEMSPVTDIDTSGIHALEDLYKSLQK 649

Query: 627 MGIQMGIVNPRIVVMEKMIASEFTDTIGKENIYLSVDDGVERCRDTVVKPKKTD 679
             IQ+ + NP   V+ K+  S F D IG + I+L+V + V+ C      PK +D
Sbjct: 650 RDIQLVLANPGPPVINKLHVSHFADLIGHDKIFLTVAEAVDSC-----SPKLSD 654

BLAST of Spg022559 vs. ExPASy TrEMBL
Match: A0A1S3CJ48 (probable sulfate transporter 3.5 OS=Cucumis melo OX=3656 GN=LOC103501545 PE=3 SV=1)

HSP 1 Score: 1091.6 bits (2822), Expect = 0.0e+00
Identity = 571/694 (82.28%), Postives = 610/694 (87.90%), Query Frame = 0

Query: 1   MGSFKSDQKVSFADPRPFGKKLKSDLKETFFPDDPFRQFQDDR-ALHRVKKGVQYFIPIL 60
           MGS K+ Q V+F+ PRPFGK+LKSDLKETFFPDDPFRQF+D+R A+HR KKG QYFIPIL
Sbjct: 1   MGSLKNGQNVNFSAPRPFGKRLKSDLKETFFPDDPFRQFRDERGAMHRAKKGFQYFIPIL 60

Query: 61  DWLPKYNFNMFKYDLLAGITITSLAIPQGISYAKLGSLPPIIGLYSSFVPPLVYAVFGSS 120
           DWLPKYN NMFKYDLLAGITITSLAIPQGISYAKLG LPPIIGLYSSFVPPL+YAVFGSS
Sbjct: 61  DWLPKYNLNMFKYDLLAGITITSLAIPQGISYAKLGVLPPIIGLYSSFVPPLIYAVFGSS 120

Query: 121 KHLAVGTVAACSLLIADIIGAVASPEKEPTLYLHLVYTATFITGVFQAALGFLRLGILVD 180
           KHLAVGTVAACSLLIA+ IG VASPE+EPTLYLHLV+TATF+TG+ QA LGFLRLGILVD
Sbjct: 121 KHLAVGTVAACSLLIAETIGGVASPEEEPTLYLHLVFTATFVTGIMQAVLGFLRLGILVD 180

Query: 181 FLSHSTILGFMGGTAVIICLQQLKGIFGLTHFTSKTDVVSVLHAVISLRYEWKWQSAVVG 240
           FLSHSTILGFMGGTAVIICLQQLKGIFGLTHFTSKTDV SVLHAV SLR EWKW+SAVVG
Sbjct: 181 FLSHSTILGFMGGTAVIICLQQLKGIFGLTHFTSKTDVYSVLHAVFSLRKEWKWESAVVG 240

Query: 241 VIFLIFLQFTRFLRNRNPKLFWVSAMAPMVTVIIGCLFAYFIKGEKHGILTAVWDVGIDW 300
           VIFL+FLQFTR+LRNRNPKLFWVSAMAPMVTVIIGCLFAYFIKG +HGILT         
Sbjct: 241 VIFLLFLQFTRYLRNRNPKLFWVSAMAPMVTVIIGCLFAYFIKGSQHGILT--------- 300

Query: 301 VGMESHVIPMFGAPDLSGVGMELWTCGFQPNSHILVGHLSKGINPISIKFLNFDSKYISA 360
                                              VGHLSKGINPISI FLNFDSKY+SA
Sbjct: 301 -----------------------------------VGHLSKGINPISIHFLNFDSKYLSA 360

Query: 361 VLQAGLITGMVALAEGIAIGRSFAIMKNEQIDGNKEMVAFGLMNIVGSFTSCYLTTGPFS 420
           V+Q GLITG+VALAEGIAIGRSFAIMKNEQIDGNKEM+AFGLMNIVGSFTSCYLTTGPFS
Sbjct: 361 VVQTGLITGLVALAEGIAIGRSFAIMKNEQIDGNKEMIAFGLMNIVGSFTSCYLTTGPFS 420

Query: 421 KTAVNFNAGCRTAMSNIVMAVFMALTLLFLAPLFSYTPLVALSAIIMSAMLGLIKYEEMY 480
           KTAVNFNAGCRTAMSN+VMA+FMALTL+FLAP+FSYTPLVALSAIIMSAMLGLIKYEEMY
Sbjct: 421 KTAVNFNAGCRTAMSNVVMAIFMALTLIFLAPVFSYTPLVALSAIIMSAMLGLIKYEEMY 480

Query: 481 HIFIVDKFDFCICMAAFLGVALLSMDVGIMLSVGLALLRALLYMARPATCKLGKIPNSAL 540
           H+  VDKFDFCICMAAFLGVA LSMDVGIMLSVGLALLRALLYMARPATCKLGKIPNS+L
Sbjct: 481 HLLKVDKFDFCICMAAFLGVAFLSMDVGIMLSVGLALLRALLYMARPATCKLGKIPNSSL 540

Query: 541 YRDMEQYPDSNRVPGIIVLQLGSPVYYANSNYIRERIFRWIRDEQAMFGSIDGSVQHVLL 600
           YRD+EQYP++ R  GIIVLQLGSP+YYANSNYIRERIFRW+RDEQ    S DG V+HVLL
Sbjct: 541 YRDVEQYPNATRTHGIIVLQLGSPIYYANSNYIRERIFRWVRDEQG--DSKDGCVEHVLL 600

Query: 601 ELSGVTSIDMTGIETLVEIRRSLQAMGIQMGIVNPRIVVMEKMIASEFTDTIGKENIYLS 660
           ELSGVTSIDMTGIETL+EIRRSLQA GIQMGIVNPRIVVMEKMIASEFT TIGKENIYLS
Sbjct: 601 ELSGVTSIDMTGIETLIEIRRSLQANGIQMGIVNPRIVVMEKMIASEFTKTIGKENIYLS 648

Query: 661 VDDGVERCRDTVVKPKKTDASERSSDVTTMEQRV 694
           VD+GVERCRD V K K+T+ S  SS+V TMEQ+V
Sbjct: 661 VDEGVERCRDLVPKLKQTETSPGSSNVRTMEQQV 648

BLAST of Spg022559 vs. ExPASy TrEMBL
Match: A0A5D3BJU3 (Putative sulfate transporter 3.5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold596G00430 PE=3 SV=1)

HSP 1 Score: 1070.5 bits (2767), Expect = 3.1e-309
Identity = 565/700 (80.71%), Postives = 604/700 (86.29%), Query Frame = 0

Query: 1   MGSFKSDQKVSFADPRPFGKKLKSDLKETFFPDDPFRQFQDDR-ALHRVKKGVQYFIPIL 60
           MGS K+ Q V+F+ PRPFGK+LKSDLKETFFPDDPFRQF+D+R A+HR KKG QYFIPIL
Sbjct: 1   MGSLKNGQNVNFSAPRPFGKRLKSDLKETFFPDDPFRQFRDERGAMHRAKKGFQYFIPIL 60

Query: 61  DWLPKYNFNMFKYDLLAGITITSLAIPQGISYAKLGSLPPIIGLYSSFVPPLVYAVFGSS 120
           DWLPKYN NMFKYDLLAGITITSLAIPQGISYAKLG LPPIIGLYSSFVPPL+YAVFGSS
Sbjct: 61  DWLPKYNLNMFKYDLLAGITITSLAIPQGISYAKLGVLPPIIGLYSSFVPPLIYAVFGSS 120

Query: 121 KHLAVGTVAACSLLIADIIGAVASPEKEPTLYLHLVYTATFITGVFQAALGFLRLGILVD 180
           KHLAVGTVAACSLLIA+ IG VASPE+EPTLYLHLV+TATF+TG+ QA LGFLRLGILVD
Sbjct: 121 KHLAVGTVAACSLLIAETIGGVASPEEEPTLYLHLVFTATFVTGIMQAVLGFLRLGILVD 180

Query: 181 FLSHSTILGFMGGTAVIICLQQLKGIFGLTHFTSKTDVVSVLHAVISLRYEWKWQSAVVG 240
           FLSHSTILGFMGGTAVIICLQQLKGIFGLTHFTSKTDV SVLHAV SLR EWKW+SAVVG
Sbjct: 181 FLSHSTILGFMGGTAVIICLQQLKGIFGLTHFTSKTDVYSVLHAVFSLRKEWKWESAVVG 240

Query: 241 VIFLIFLQFTRFLRNRNPKLFWVSAMAPMVTVIIGCLFAYFIKGEKHGILTAVWDVGIDW 300
           VIFL+FLQFTR+LRNRNPKLFWVSAMAPMVTVIIGCLFAYFIKG +HGILT         
Sbjct: 241 VIFLLFLQFTRYLRNRNPKLFWVSAMAPMVTVIIGCLFAYFIKGSQHGILT--------- 300

Query: 301 VGMESHVIPMFGAPDLSGVGMELWTCGFQPNSHILVGHLSKGINPISIKFLNFDSKYISA 360
                                              VGHLSKGINPISI FLNFDSKY+SA
Sbjct: 301 -----------------------------------VGHLSKGINPISIHFLNFDSKYLSA 360

Query: 361 VLQAGLITGMVALAEGIAIGRSFAIMKNEQIDGNKEMVAFGLMNIVGSFTSCYLTTGPFS 420
           V+Q GLITG+VALAEGIAIGRSFAIMKNEQIDGNKEM+AFGLMNIVGSFTSCYLTTGPFS
Sbjct: 361 VVQTGLITGLVALAEGIAIGRSFAIMKNEQIDGNKEMIAFGLMNIVGSFTSCYLTTGPFS 420

Query: 421 KTAVNFNAGCRTAMSNIVMAVFMALTLLFLAPLFSYTPLVALSAIIMSAMLGLIKYEEMY 480
           KTAVNFNAGCRTAMSN+VMA+FMALTL+FLAP+FSYTPLVALSAIIMSAMLGLIKYEEMY
Sbjct: 421 KTAVNFNAGCRTAMSNVVMAIFMALTLIFLAPVFSYTPLVALSAIIMSAMLGLIKYEEMY 480

Query: 481 HIFIVDKFDFCICMAAFLGVALLSMDVGIMLSVGLALLRALLYMARPATCKLGKIPNSAL 540
           H+  VDKFDFCICMAAFLGVA LSMDVGIMLSVGLALLRALLYMARPATCKLGKIPNS+L
Sbjct: 481 HLLKVDKFDFCICMAAFLGVAFLSMDVGIMLSVGLALLRALLYMARPATCKLGKIPNSSL 540

Query: 541 YRDMEQYPDSNRVPGIIVLQLGSPVYYANSNYIRERIFRWIRDEQAMFGSIDGSVQHVLL 600
           YRD+EQYP++ R  GIIVLQLGSP+YYANSNYIRERIFRW+RDEQ    S DG V+HVLL
Sbjct: 541 YRDVEQYPNATRTHGIIVLQLGSPIYYANSNYIRERIFRWVRDEQG--DSKDGCVEHVLL 600

Query: 601 ELSGVTSIDMTGIETLVEIRRSLQAMGIQMGIVNPRIVVMEKMIASEFTDTIGKENIYLS 660
           ELSGVTSIDMTGIETL+EIRRSLQA GIQMGIVNPRIVVMEKMIASEFT TIGKENIYLS
Sbjct: 601 ELSGVTSIDMTGIETLIEIRRSLQANGIQMGIVNPRIVVMEKMIASEFTKTIGKENIYLS 654

Query: 661 VDDGVER--CR----DTVVKPKKTDASERSSDVTTMEQRV 694
           VD+GVER  CR      V KP  +    RSS + T+ Q +
Sbjct: 661 VDEGVERSVCRKSLLSNVAKPTPSSKLYRSSSLPTLCQTI 654

BLAST of Spg022559 vs. ExPASy TrEMBL
Match: A0A5A7TZ58 (Putative sulfate transporter 3.5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold114G001580 PE=3 SV=1)

HSP 1 Score: 1070.5 bits (2767), Expect = 3.1e-309
Identity = 565/700 (80.71%), Postives = 604/700 (86.29%), Query Frame = 0

Query: 1   MGSFKSDQKVSFADPRPFGKKLKSDLKETFFPDDPFRQFQDDR-ALHRVKKGVQYFIPIL 60
           MGS K+ Q V+F+ PRPFGK+LKSDLKETFFPDDPFRQF+D+R A+HR KKG QYFIPIL
Sbjct: 1   MGSLKNGQNVNFSAPRPFGKRLKSDLKETFFPDDPFRQFRDERGAMHRAKKGFQYFIPIL 60

Query: 61  DWLPKYNFNMFKYDLLAGITITSLAIPQGISYAKLGSLPPIIGLYSSFVPPLVYAVFGSS 120
           DWLPKYN NMFKYDLLAGITITSLAIPQGISYAKLG LPPIIGLYSSFVPPL+YAVFGSS
Sbjct: 61  DWLPKYNLNMFKYDLLAGITITSLAIPQGISYAKLGVLPPIIGLYSSFVPPLIYAVFGSS 120

Query: 121 KHLAVGTVAACSLLIADIIGAVASPEKEPTLYLHLVYTATFITGVFQAALGFLRLGILVD 180
           KHLAVGTVAACSLLIA+ IG VASPE+EPTLYLHLV+TATF+TG+ QA LGFLRLGILVD
Sbjct: 121 KHLAVGTVAACSLLIAETIGGVASPEEEPTLYLHLVFTATFVTGIMQAVLGFLRLGILVD 180

Query: 181 FLSHSTILGFMGGTAVIICLQQLKGIFGLTHFTSKTDVVSVLHAVISLRYEWKWQSAVVG 240
           FLSHSTILGFMGGTAVIICLQQLKGIFGLTHFTSKTDV SVLHAV SLR EWKW+SAVVG
Sbjct: 181 FLSHSTILGFMGGTAVIICLQQLKGIFGLTHFTSKTDVYSVLHAVFSLRKEWKWESAVVG 240

Query: 241 VIFLIFLQFTRFLRNRNPKLFWVSAMAPMVTVIIGCLFAYFIKGEKHGILTAVWDVGIDW 300
           VIFL+FLQFTR+LRNRNPKLFWVSAMAPMVTVIIGCLFAYFIKG +HGILT         
Sbjct: 241 VIFLLFLQFTRYLRNRNPKLFWVSAMAPMVTVIIGCLFAYFIKGSQHGILT--------- 300

Query: 301 VGMESHVIPMFGAPDLSGVGMELWTCGFQPNSHILVGHLSKGINPISIKFLNFDSKYISA 360
                                              VGHLSKGINPISI FLNFDSKY+SA
Sbjct: 301 -----------------------------------VGHLSKGINPISIHFLNFDSKYLSA 360

Query: 361 VLQAGLITGMVALAEGIAIGRSFAIMKNEQIDGNKEMVAFGLMNIVGSFTSCYLTTGPFS 420
           V+Q GLITG+VALAEGIAIGRSFAIMKNEQIDGNKEM+AFGLMNIVGSFTSCYLTTGPFS
Sbjct: 361 VVQTGLITGLVALAEGIAIGRSFAIMKNEQIDGNKEMIAFGLMNIVGSFTSCYLTTGPFS 420

Query: 421 KTAVNFNAGCRTAMSNIVMAVFMALTLLFLAPLFSYTPLVALSAIIMSAMLGLIKYEEMY 480
           KTAVNFNAGCRTAMSN+VMA+FMALTL+FLAP+FSYTPLVALSAIIMSAMLGLIKYEEMY
Sbjct: 421 KTAVNFNAGCRTAMSNVVMAIFMALTLIFLAPVFSYTPLVALSAIIMSAMLGLIKYEEMY 480

Query: 481 HIFIVDKFDFCICMAAFLGVALLSMDVGIMLSVGLALLRALLYMARPATCKLGKIPNSAL 540
           H+  VDKFDFCICMAAFLGVA LSMDVGIMLSVGLALLRALLYMARPATCKLGKIPNS+L
Sbjct: 481 HLLKVDKFDFCICMAAFLGVAFLSMDVGIMLSVGLALLRALLYMARPATCKLGKIPNSSL 540

Query: 541 YRDMEQYPDSNRVPGIIVLQLGSPVYYANSNYIRERIFRWIRDEQAMFGSIDGSVQHVLL 600
           YRD+EQYP++ R  GIIVLQLGSP+YYANSNYIRERIFRW+RDEQ    S DG V+HVLL
Sbjct: 541 YRDVEQYPNATRTHGIIVLQLGSPIYYANSNYIRERIFRWVRDEQG--DSKDGCVEHVLL 600

Query: 601 ELSGVTSIDMTGIETLVEIRRSLQAMGIQMGIVNPRIVVMEKMIASEFTDTIGKENIYLS 660
           ELSGVTSIDMTGIETL+EIRRSLQA GIQMGIVNPRIVVMEKMIASEFT TIGKENIYLS
Sbjct: 601 ELSGVTSIDMTGIETLIEIRRSLQANGIQMGIVNPRIVVMEKMIASEFTKTIGKENIYLS 654

Query: 661 VDDGVER--CR----DTVVKPKKTDASERSSDVTTMEQRV 694
           VD+GVER  CR      V KP  +    RSS + T+ Q +
Sbjct: 661 VDEGVERSVCRKSLLSNVAKPTPSSKLYRSSSLPTLCQTI 654

BLAST of Spg022559 vs. ExPASy TrEMBL
Match: A0A6J1EB41 (probable sulfate transporter 3.5 OS=Cucurbita moschata OX=3662 GN=LOC111432501 PE=3 SV=1)

HSP 1 Score: 1062.0 bits (2745), Expect = 1.1e-306
Identity = 550/693 (79.37%), Postives = 601/693 (86.72%), Query Frame = 0

Query: 1   MGSFKSDQKVSFADPRPFGKKLKSDLKETFFPDDPFRQFQDDRALHRVKKGVQYFIPILD 60
           MGS KS+Q V+FA PRPF K+LKSDLKETF+PDDPFRQF+ DR  H++KK VQYFIPIL+
Sbjct: 1   MGSLKSEQHVNFAAPRPFVKRLKSDLKETFYPDDPFRQFRGDRRTHQLKKAVQYFIPILE 60

Query: 61  WLPKYNFNMFKYDLLAGITITSLAIPQGISYAKLGSLPPIIGLYSSFVPPLVYAVFGSSK 120
           WLPKYNFNMFKYDLLAGITITSLAIPQGISYAKLGSLPPIIGLYSSFVPPLVYAVFGSSK
Sbjct: 61  WLPKYNFNMFKYDLLAGITITSLAIPQGISYAKLGSLPPIIGLYSSFVPPLVYAVFGSSK 120

Query: 121 HLAVGTVAACSLLIADIIGAVASPEKEPTLYLHLVYTATFITGVFQAALGFLRLGILVDF 180
           HLAVGTVAACSLLIA+IIG VASPE++PTLYLHLV TAT ITGV Q ALG LRLGILVDF
Sbjct: 121 HLAVGTVAACSLLIAEIIGEVASPEEDPTLYLHLVLTATLITGVMQTALGILRLGILVDF 180

Query: 181 LSHSTILGFMGGTAVIICLQQLKGIFGLTHFTSKTDVVSVLHAVISLRYEWKWQSAVVGV 240
           LSHSTI+GFMGGTAVIICLQQLKGIFGLTHFTSKTDV SVLHAV SLR+EW+WQSAV GV
Sbjct: 181 LSHSTIIGFMGGTAVIICLQQLKGIFGLTHFTSKTDVYSVLHAVFSLRHEWRWQSAVTGV 240

Query: 241 IFLIFLQFTRFLRNRNPKLFWVSAMAPMVTVIIGCLFAYFIKGEKHGILTAVWDVGIDWV 300
           +FL+FLQFTR+LR+RNPKLFWVSAMAPMVTVI+GCL AY + G +HGILT          
Sbjct: 241 VFLVFLQFTRYLRDRNPKLFWVSAMAPMVTVIVGCLVAYLVHGSQHGILT---------- 300

Query: 301 GMESHVIPMFGAPDLSGVGMELWTCGFQPNSHILVGHLSKGINPISIKFLNFDSKYISAV 360
                                             VG L+KGINP SI  LNFD KY+ AV
Sbjct: 301 ----------------------------------VGRLNKGINPPSIHSLNFDPKYLPAV 360

Query: 361 LQAGLITGMVALAEGIAIGRSFAIMKNEQIDGNKEMVAFGLMNIVGSFTSCYLTTGPFSK 420
           +QAG ITG+VALAEGIAIGRSFAIMKNEQ+DGNKEMVAFGLMNIVGSFTSCYLTTGPFSK
Sbjct: 361 VQAGAITGLVALAEGIAIGRSFAIMKNEQVDGNKEMVAFGLMNIVGSFTSCYLTTGPFSK 420

Query: 421 TAVNFNAGCRTAMSNIVMAVFMALTLLFLAPLFSYTPLVALSAIIMSAMLGLIKYEEMYH 480
           TAVNFNAGCRTAMSN+VMAVFMALTLLFLAPLFSYTPLVALSAIIMSAM GLIKYEE+YH
Sbjct: 421 TAVNFNAGCRTAMSNVVMAVFMALTLLFLAPLFSYTPLVALSAIIMSAMFGLIKYEEIYH 480

Query: 481 IFIVDKFDFCICMAAFLGVALLSMDVGIMLSVGLALLRALLYMARPATCKLGKIPNSALY 540
           +F VDKFDFCICMAAFLGVALLSMDVG+MLSVGLALLRALLYMARPATCKL KIP++ LY
Sbjct: 481 LFKVDKFDFCICMAAFLGVALLSMDVGLMLSVGLALLRALLYMARPATCKLEKIPDTDLY 540

Query: 541 RDMEQYPDSNRVPGIIVLQLGSPVYYANSNYIRERIFRWIRDEQAMFGSIDGSVQHVLLE 600
           RD+EQYP++ RVPG+IVLQLGSP+YYANSNYIRERI RW+RDE A F S+DGSVQHVLLE
Sbjct: 541 RDVEQYPNAMRVPGVIVLQLGSPIYYANSNYIRERILRWVRDEHAKFDSVDGSVQHVLLE 600

Query: 601 LSGVTSIDMTGIETLVEIRRSLQAMGIQMGIVNPRIVVMEKMIASEFTDTIGKENIYLSV 660
           LSGVTSIDMTGIETL+EIRR LQA G++MGIVNPRIVVMEKMIASEFT+TIGKENIYLSV
Sbjct: 601 LSGVTSIDMTGIETLIEIRRLLQANGVKMGIVNPRIVVMEKMIASEFTNTIGKENIYLSV 649

Query: 661 DDGVERCRDTVVKPKKTDASERSSDVTTMEQRV 694
           DDGVERCRD    P+KT+ S+ + +++ +E RV
Sbjct: 661 DDGVERCRDLAYNPQKTNPSDDNVELSAVELRV 649

BLAST of Spg022559 vs. ExPASy TrEMBL
Match: A0A6J1IAJ9 (probable sulfate transporter 3.5 OS=Cucurbita maxima OX=3661 GN=LOC111471645 PE=3 SV=1)

HSP 1 Score: 1061.2 bits (2743), Expect = 1.8e-306
Identity = 550/693 (79.37%), Postives = 597/693 (86.15%), Query Frame = 0

Query: 1   MGSFKSDQKVSFADPRPFGKKLKSDLKETFFPDDPFRQFQDDRALHRVKKGVQYFIPILD 60
           MGS KS+Q VSFA PRPF  +LKSDLKETF+PDDPFRQF+DD+  H++KK VQYFIPIL+
Sbjct: 1   MGSLKSEQNVSFAAPRPFLNRLKSDLKETFYPDDPFRQFRDDKPTHQLKKAVQYFIPILE 60

Query: 61  WLPKYNFNMFKYDLLAGITITSLAIPQGISYAKLGSLPPIIGLYSSFVPPLVYAVFGSSK 120
           WLPKYNFNMFKYDLLAGITITSLAIPQGISYAKLGSLPPIIGLYSSFVPPLVYAVFGSSK
Sbjct: 61  WLPKYNFNMFKYDLLAGITITSLAIPQGISYAKLGSLPPIIGLYSSFVPPLVYAVFGSSK 120

Query: 121 HLAVGTVAACSLLIADIIGAVASPEKEPTLYLHLVYTATFITGVFQAALGFLRLGILVDF 180
           HLAVGTVAACSLLIA+IIG VASPE++PTLYLHLV TAT ITGV Q ALG LRLGILVDF
Sbjct: 121 HLAVGTVAACSLLIAEIIGEVASPEEDPTLYLHLVLTATLITGVMQTALGILRLGILVDF 180

Query: 181 LSHSTILGFMGGTAVIICLQQLKGIFGLTHFTSKTDVVSVLHAVISLRYEWKWQSAVVGV 240
           LSHSTILGFMGGTAVIICLQQLKGIFGLTHFTSKTDV SVLHAV SL+ EW+WQSAV GV
Sbjct: 181 LSHSTILGFMGGTAVIICLQQLKGIFGLTHFTSKTDVYSVLHAVFSLKNEWRWQSAVTGV 240

Query: 241 IFLIFLQFTRFLRNRNPKLFWVSAMAPMVTVIIGCLFAYFIKGEKHGILTAVWDVGIDWV 300
           +FL+FLQFTR+LR+RNPKLFWVSAMAPMVTVI+GCL AY + G +HGILT          
Sbjct: 241 VFLVFLQFTRYLRDRNPKLFWVSAMAPMVTVIVGCLVAYLVHGSQHGILT---------- 300

Query: 301 GMESHVIPMFGAPDLSGVGMELWTCGFQPNSHILVGHLSKGINPISIKFLNFDSKYISAV 360
                                             VG L+KGINP SI  LNFD KY+SAV
Sbjct: 301 ----------------------------------VGRLNKGINPPSIHSLNFDPKYLSAV 360

Query: 361 LQAGLITGMVALAEGIAIGRSFAIMKNEQIDGNKEMVAFGLMNIVGSFTSCYLTTGPFSK 420
           +QAG ITG+VALAEGIAIGRSFAIMKNEQ+DGNKEMVAFGLMNIVGSFTSCYLTTGPFSK
Sbjct: 361 VQAGAITGLVALAEGIAIGRSFAIMKNEQVDGNKEMVAFGLMNIVGSFTSCYLTTGPFSK 420

Query: 421 TAVNFNAGCRTAMSNIVMAVFMALTLLFLAPLFSYTPLVALSAIIMSAMLGLIKYEEMYH 480
           TAVNFNAGCRT MSN+VMA+FMALTLLFLAPLFSYTPLVALSAIIMSAM GLIKYEE+YH
Sbjct: 421 TAVNFNAGCRTPMSNVVMAIFMALTLLFLAPLFSYTPLVALSAIIMSAMFGLIKYEEIYH 480

Query: 481 IFIVDKFDFCICMAAFLGVALLSMDVGIMLSVGLALLRALLYMARPATCKLGKIPNSALY 540
           +F VDKFDFCICMAAFLGVALLSMDVG+MLSVGLALLRALLYMARPATCKLGKIP++ LY
Sbjct: 481 LFKVDKFDFCICMAAFLGVALLSMDVGLMLSVGLALLRALLYMARPATCKLGKIPDTDLY 540

Query: 541 RDMEQYPDSNRVPGIIVLQLGSPVYYANSNYIRERIFRWIRDEQAMFGSIDGSVQHVLLE 600
           RD EQYP++ RVPGIIVLQLGSP+YYANSNYIRERI RW+RDE A F S+DGSV HVLLE
Sbjct: 541 RDTEQYPNAMRVPGIIVLQLGSPIYYANSNYIRERILRWVRDEHAEFDSVDGSVHHVLLE 600

Query: 601 LSGVTSIDMTGIETLVEIRRSLQAMGIQMGIVNPRIVVMEKMIASEFTDTIGKENIYLSV 660
           LSGVTSIDMTGIETLVEIRR LQA G++MG+VNPRIVVMEKMIASEFT+TIGKENIYLSV
Sbjct: 601 LSGVTSIDMTGIETLVEIRRLLQANGVKMGVVNPRIVVMEKMIASEFTNTIGKENIYLSV 649

Query: 661 DDGVERCRDTVVKPKKTDASERSSDVTTMEQRV 694
           DDGVERCRD    P KT+ S+ + ++  +E RV
Sbjct: 661 DDGVERCRDLAYNPHKTNLSDENVELNAVEHRV 649

BLAST of Spg022559 vs. TAIR 10
Match: AT5G19600.1 (sulfate transporter 3;5 )

HSP 1 Score: 820.1 bits (2117), Expect = 1.4e-237
Identity = 422/669 (63.08%), Postives = 512/669 (76.53%), Query Frame = 0

Query: 10  VSFADPRPFGKKLKSDLKETFFPDDPFRQF-QDDRALHRVKKGVQYFIPILDWLPKYNFN 69
           V+F+ PR FG K KS  KETFFPDDPF+   Q+   L + KK ++YF+PI +WLPKY+  
Sbjct: 17  VNFSTPRGFGSKFKSKCKETFFPDDPFKPISQEPNRLLKTKKLLEYFVPIFEWLPKYDMQ 76

Query: 70  MFKYDLLAGITITSLAIPQGISYAKLGSLPPIIGLYSSFVPPLVYAVFGSSKHLAVGTVA 129
             KYD+LAGITITSLA+PQGISYAKL S+PPIIGLYSSFVPP VYAVFGSS +LAVGTVA
Sbjct: 77  KLKYDVLAGITITSLAVPQGISYAKLASIPPIIGLYSSFVPPFVYAVFGSSNNLAVGTVA 136

Query: 130 ACSLLIADIIGAVASPEKEPTLYLHLVYTATFITGVFQAALGFLRLGILVDFLSHSTILG 189
           ACSLLIA+  G     + EP LYLHL++TAT ITG+FQ A+GFLRLGILVDFLSHSTI G
Sbjct: 137 ACSLLIAETFGE-EMIKNEPELYLHLIFTATLITGLFQFAMGFLRLGILVDFLSHSTITG 196

Query: 190 FMGGTAVIICLQQLKGIFGLTHFTSKTDVVSVLHAVISLRYEWKWQSAVVGVIFLIFLQF 249
           FMGGTA+II LQQLKGIFGL HFT KTDVVSVLH+++  R EWKWQS + GV FL+FLQ 
Sbjct: 197 FMGGTAIIILLQQLKGIFGLVHFTHKTDVVSVLHSILDNRAEWKWQSTLAGVCFLVFLQS 256

Query: 250 TRFLRNRNPKLFWVSAMAPMVTVIIGCLFAYFIKGEKHGILTAVWDVGIDWVGMESHVIP 309
           TR+++ R PKLFWVSAM PMV V++GC+ AY +KG  HGI T                  
Sbjct: 257 TRYIKQRYPKLFWVSAMGPMVVVVVGCVVAYLVKGTAHGIAT------------------ 316

Query: 310 MFGAPDLSGVGMELWTCGFQPNSHILVGHLSKGINPISIKFLNFDSKYISAVLQAGLITG 369
                                     VG L KG+NP SI+ LNFDSKY+  V +AG++TG
Sbjct: 317 --------------------------VGPLKKGLNPPSIQLLNFDSKYLGMVFKAGIVTG 376

Query: 370 MVALAEGIAIGRSFAIMKNEQIDGNKEMVAFGLMNIVGSFTSCYLTTGPFSKTAVNFNAG 429
           ++ALAEGIAIGRSFA+MKNEQ DGNKEM+AFGLMN++GSFTSCYLTTGPFSKTAVN+NAG
Sbjct: 377 LIALAEGIAIGRSFAVMKNEQTDGNKEMIAFGLMNVIGSFTSCYLTTGPFSKTAVNYNAG 436

Query: 430 CRTAMSNIVMAVFMALTLLFLAPLFSYTPLVALSAIIMSAMLGLIKYEEMYHIFIVDKFD 489
            +T MSN+VM V M L LLFLAPLFSYTPLV LSAIIMSAMLGLI YEEMYH+F VDKFD
Sbjct: 437 TKTPMSNVVMGVCMMLVLLFLAPLFSYTPLVGLSAIIMSAMLGLINYEEMYHLFKVDKFD 496

Query: 490 FCICMAAFLGVALLSMDVGIMLSVGLALLRALLYMARPATCKLGKIPNSALYRDMEQYPD 549
           F +CM+AF GV+ LSMD G+++SVG +++RALLY+ARP+TCKLG+IPNS ++RD+EQYP 
Sbjct: 497 FLVCMSAFFGVSFLSMDYGLIISVGFSIVRALLYVARPSTCKLGRIPNSVMFRDIEQYPA 556

Query: 550 SNRVPGIIVLQLGSPVYYANSNYIRERIFRWIRDEQAMFGSIDGSVQHVLLELSGVTSID 609
           S  + G I+LQLGSPV++ANS Y+RERI RWIRDE         +++ +LL+LSGV++ID
Sbjct: 557 SEEMLGYIILQLGSPVFFANSTYVRERILRWIRDEPE-------AIEFLLLDLSGVSTID 616

Query: 610 MTGIETLVEIRRSLQAMGIQMGIVNPRIVVMEKMIASEFTDTIGKENIYLSVDDGVERCR 669
           MTG+ETL+EI+R L +  I+M I+NPR  V+EKM+ S F + IGKE ++LS+DD V+ CR
Sbjct: 617 MTGMETLLEIQRILGSKNIKMVIINPRFEVLEKMMLSHFVEKIGKEYMFLSIDDAVQACR 633

Query: 670 D--TVVKPK 676
              T  KP+
Sbjct: 677 FNLTTTKPE 633

BLAST of Spg022559 vs. TAIR 10
Match: AT3G51895.1 (sulfate transporter 3;1 )

HSP 1 Score: 681.4 bits (1757), Expect = 7.6e-196
Identity = 344/660 (52.12%), Postives = 468/660 (70.91%), Query Frame = 0

Query: 10  VSFADPRPFGKKLKSDLKETFFPDDPFRQFQDDRALHRVKKGVQYFIPILDWLPKYNFNM 69
           V    P+PF K L+  +KET FPDDPFRQF++  A  +   G++YF+PI +W P+YN   
Sbjct: 22  VEAPQPQPFLKSLQYSVKETLFPDDPFRQFKNQNASRKFVLGLKYFLPIFEWAPRYNLKF 81

Query: 70  FKYDLLAGITITSLAIPQGISYAKLGSLPPIIGLYSSFVPPLVYAVFGSSKHLAVGTVAA 129
           FK DL+AGITI SLAIPQGISYAKL +LPPI+GLYSSFVPPLVYAV GSS+ LAVGTVA 
Sbjct: 82  FKSDLIAGITIASLAIPQGISYAKLANLPPILGLYSSFVPPLVYAVLGSSRDLAVGTVAV 141

Query: 130 CSLLIADIIGAVASPEKEPTLYLHLVYTATFITGVFQAALGFLRLGILVDFLSHSTILGF 189
            SLL   ++      EK+P LYLHL +TATF  GV +A+LG  RLG +VDFLSH+TI+GF
Sbjct: 142 ASLLTGAMLSKEVDAEKDPKLYLHLAFTATFFAGVLEASLGIFRLGFIVDFLSHATIVGF 201

Query: 190 MGGTAVIICLQQLKGIFGLTHFTSKTDVVSVLHAVISLRYEWKWQSAVVGVIFLIFLQFT 249
           MGG A ++ LQQLKGIFGL HFT  TDV+SV+ +V S  +EW+W+S V+G  FL FL  T
Sbjct: 202 MGGAATVVSLQQLKGIFGLKHFTDSTDVISVMRSVFSQTHEWRWESGVLGCGFLFFLLST 261

Query: 250 RFLRNRNPKLFWVSAMAPMVTVIIGCLFAYFIKGEKHGILTAVWDVGIDWVGMESHVIPM 309
           R+   + PK FWV+AMAP+ +VI+G L  YF   E+HG+                     
Sbjct: 262 RYFSIKKPKFFWVAAMAPLTSVILGSLLVYFTHAERHGVQ-------------------- 321

Query: 310 FGAPDLSGVGMELWTCGFQPNSHILVGHLSKGINPISIKFLNFDSKYISAVLQAGLITGM 369
                                   ++G L KG+NP+S   L F S Y+S  ++ GLITG+
Sbjct: 322 ------------------------VIGDLKKGLNPLSGSDLIFTSPYMSTAVKTGLITGI 381

Query: 370 VALAEGIAIGRSFAIMKNEQIDGNKEMVAFGLMNIVGSFTSCYLTTGPFSKTAVNFNAGC 429
           +ALAEG+A+GRSFA+ KN  IDGNKEM+AFG+MNIVGSFTSCYLTTGPFS++AVN+NAGC
Sbjct: 382 IALAEGVAVGRSFAMFKNYNIDGNKEMIAFGMMNIVGSFTSCYLTTGPFSRSAVNYNAGC 441

Query: 430 RTAMSNIVMAVFMALTLLFLAPLFSYTPLVALSAIIMSAMLGLIKYEEMYHIFIVDKFDF 489
           +TAMSNIVMA+ +  TLLFL PLF YTPLV LSAII+SAMLGLI Y+   H++ VDKFDF
Sbjct: 442 KTAMSNIVMAIAVMFTLLFLTPLFHYTPLVVLSAIIISAMLGLIDYQAAIHLWKVDKFDF 501

Query: 490 CICMAAFLGVALLSMDVGIMLSVGLALLRALLYMARPATCKLGKIPNSALYRDMEQYPDS 549
            +CM+A++GV   S+++G++++V +++ R LL+++RP T   G IPNS +YR+ EQYP S
Sbjct: 502 LVCMSAYVGVVFGSVEIGLVVAVAISIARLLLFVSRPKTAVKGNIPNSMIYRNTEQYPSS 561

Query: 550 NRVPGIIVLQLGSPVYYANSNYIRERIFRWI-RDEQAMFGSIDGSVQHVLLELSGVTSID 609
             VPGI++L++ +P+Y+AN++Y+RERI RWI  +E+ +  S + S+Q+++L++S V +ID
Sbjct: 562 RTVPGILILEIDAPIYFANASYLRERIIRWIDEEEERVKQSGESSLQYIILDMSAVGNID 621

Query: 610 MTGIETLVEIRRSLQAMGIQMGIVNPRIVVMEKMIASEFT-DTIGKENIYLSVDDGVERC 668
            +GI  +VEI++ +    +++ + NP+  V++K+  S+F  D +GKE ++L+V + VE C
Sbjct: 622 TSGISMMVEIKKVIDRRALKLVLSNPKGEVVKKLTRSKFIGDHLGKEWMFLTVGEAVEAC 637

BLAST of Spg022559 vs. TAIR 10
Match: AT4G02700.1 (sulfate transporter 3;2 )

HSP 1 Score: 625.2 bits (1611), Expect = 6.4e-179
Identity = 325/683 (47.58%), Postives = 463/683 (67.79%), Query Frame = 0

Query: 1   MGSFKSDQ--KVSFADPRPFGKKLKSDLKETFFPDDPFRQFQDD-RALHRVKKGVQYFIP 60
           M S ++ Q  +V    P+PF K LK+ L E  F DDPFR+ +++ +   +++ G+++  P
Sbjct: 1   MSSKRASQYHQVEIPPPQPFLKSLKNTLNEILFADDPFRRIRNESKTSKKIELGLRHVFP 60

Query: 61  ILDWLPKYNFNMFKYDLLAGITITSLAIPQGISYAKLGSLPPIIGLYSSFVPPLVYAVFG 120
           IL+W   Y+    K D+++GITI SLAIPQGISYA+L +LPPI+GLYSS VPPLVYA+ G
Sbjct: 61  ILEWARGYSLEYLKSDVISGITIASLAIPQGISYAQLANLPPILGLYSSLVPPLVYAIMG 120

Query: 121 SSKHLAVGTVAACSLLIADIIGAVASPEKEPTLYLHLVYTATFITGVFQAALGFLRLGIL 180
           SS+ LAVGTVA  SLL A ++G   +    P LYLHL +TATF  G+ Q  LG LRLG +
Sbjct: 121 SSRDLAVGTVAVASLLTAAMLGKEVNAVVNPKLYLHLAFTATFFAGLMQTCLGLLRLGFV 180

Query: 181 VDFLSHSTILGFMGGTAVIICLQQLKGIFGLTHFTSKTDVVSVLHAVISLRYEWKWQSAV 240
           V+ LSH+ I+GFMGG A ++CLQQLKG+ GL HFT  TD+V+VL ++ S  + W+W+S V
Sbjct: 181 VEILSHAAIVGFMGGAATVVCLQQLKGLLGLHHFTHSTDIVTVLRSIFSQSHMWRWESGV 240

Query: 241 VGVIFLIFLQFTRFLRNRNPKLFWVSAMAPMVTVIIGCLFAYFIKGEKHGILTAVWDVGI 300
           +G  FLIFL  T+++  + PKLFW+SAM+P+V+VI G +F YF+  + HGI         
Sbjct: 241 LGCCFLIFLLTTKYISKKRPKLFWISAMSPLVSVIFGTIFLYFLHDQFHGIQ-------- 300

Query: 301 DWVGMESHVIPMFGAPDLSGVGMELWTCGFQPNSHILVGHLSKGINPISIKFLNFDSKYI 360
                                                +G L KGINP SI  L F   Y+
Sbjct: 301 ------------------------------------FIGELKKGINPPSITHLVFTPPYV 360

Query: 361 SAVLQAGLITGMVALAEGIAIGRSFAIMKNEQIDGNKEMVAFGLMNIVGSFTSCYLTTGP 420
              L+ G+ITG++ALAEGIA+GRSFA+ KN  IDGNKEM+AFG+MNI+GSF+SCYLTTGP
Sbjct: 361 MLALKVGIITGVIALAEGIAVGRSFAMYKNYNIDGNKEMIAFGMMNILGSFSSCYLTTGP 420

Query: 421 FSKTAVNFNAGCRTAMSNIVMAVFMALTLLFLAPLFSYTPLVALSAIIMSAMLGLIKYEE 480
           FS++AVN+NAGC+TA+SN+VMAV +A+TLLFL PLF YTPLV LS+II++AMLGL+ YE 
Sbjct: 421 FSRSAVNYNAGCKTALSNVVMAVAVAVTLLFLTPLFFYTPLVVLSSIIIAAMLGLVDYEA 480

Query: 481 MYHIFIVDKFDFCICMAAFLGVALLSMDVGIMLSVGLALLRALLYMARPATCKLGKIPNS 540
             H++ +DKFDF +C++A+LGV   ++++G++LSVG++++R +L++ RP    +G I NS
Sbjct: 481 AIHLWKLDKFDFFVCLSAYLGVVFGTIEIGLILSVGISVMRLVLFVGRPKIYVMGNIQNS 540

Query: 541 ALYRDMEQYPDSNRVPGIIVLQLGSPVYYANSNYIRERIFRWI-RDEQAMFGSIDGSVQH 600
            +YR++E YP +     +++L +  P+Y+ANS Y+R+RI RWI  +E  +  S D S+Q+
Sbjct: 541 EIYRNIEHYPQAITRSSLLILHIDGPIYFANSTYLRDRIGRWIDEEEDKLRTSGDISLQY 600

Query: 601 VLLELSGVTSIDMTGIETLVEIRRSLQAMGIQMGIVNPRIVVMEKMIASEFTDTIGKENI 660
           ++L++S V +ID +GI  L E+ + L    +++ I NP   VM+K+  S F ++IGKE I
Sbjct: 601 IVLDMSAVGNIDTSGISMLEELNKILGRRELKLVIANPGAEVMKKLSKSTFIESIGKERI 638

Query: 661 YLSVDDGVERCRDTVVKPKKTDA 680
           YL+V + V  C D ++   K D+
Sbjct: 661 YLTVAEAVAAC-DFMLHTAKPDS 638

BLAST of Spg022559 vs. TAIR 10
Match: AT4G08620.1 (sulphate transporter 1;1 )

HSP 1 Score: 580.1 bits (1494), Expect = 2.4e-165
Identity = 304/656 (46.34%), Postives = 427/656 (65.09%), Query Frame = 0

Query: 20  KKLKSDLKETFFPDDPFRQFQDDRALHRVKKGVQYFIPILDWLPKYNFNMFKYDLLAGIT 79
           K +KS ++ETFF D P R F+      +   G+Q   PI+ W  +Y    F+ DL+AG+T
Sbjct: 35  KDIKSVVEETFFHDAPLRDFKGQTPAKKALLGIQAVFPIIGWAREYTLRKFRGDLIAGLT 94

Query: 80  ITSLAIPQGISYAKLGSLPPIIGLYSSFVPPLVYAVFGSSKHLAVGTVAACSLLIADIIG 139
           I SL IPQ I YAKL ++ P  GLYSSFVPPL+YA  GSS+ +A+G VA  SLL+  +  
Sbjct: 95  IASLCIPQDIGYAKLANVDPKYGLYSSFVPPLIYAGMGSSRDIAIGPVAVVSLLVGTLCQ 154

Query: 140 AVASPEKEPTLYLHLVYTATFITGVFQAALGFLRLGILVDFLSHSTILGFMGGTAVIICL 199
           AV  P+K P  YL LV+TATF  G+FQA LGFLRLG L+DFLSH+ ++GFMGG A+ I L
Sbjct: 155 AVIDPKKNPEDYLRLVFTATFFAGIFQAGLGFLRLGFLIDFLSHAAVVGFMGGAAITIAL 214

Query: 200 QQLKGIFGLTHFTSKTDVVSVLHAVI-SLRYEWKWQSAVVGVIFLIFLQFTRFLRNRNPK 259
           QQLKG  G+  FT KTD+VSV+H+V  +  + W WQ+ V+G  FL FL  T+F+  RN K
Sbjct: 215 QQLKGFLGIKTFTKKTDIVSVMHSVFKNAEHGWNWQTIVIGASFLTFLLVTKFIGKRNRK 274

Query: 260 LFWVSAMAPMVTVIIGCLFAYFIKGEKHGILTAVWDVGIDWVGMESHVIPMFGAPDLSGV 319
           LFWV A+AP+++VII   F +  + +K G+                              
Sbjct: 275 LFWVPAIAPLISVIISTFFVFIFRADKQGVQ----------------------------- 334

Query: 320 GMELWTCGFQPNSHILVGHLSKGINPISIKFLNFDSKYISAVLQAGLITGMVALAEGIAI 379
                          +V H+ +GINPIS+  + F  KY +  ++ G I GMVAL E +AI
Sbjct: 335 ---------------IVKHIDQGINPISVHKIFFSGKYFTEGIRIGGIAGMVALTEAVAI 394

Query: 380 GRSFAIMKNEQIDGNKEMVAFGLMNIVGSFTSCYLTTGPFSKTAVNFNAGCRTAMSNIVM 439
            R+FA MK+ QIDGNKEM+A G MN+VGS TSCY+ TG FS++AVNF AG  TA+SNIVM
Sbjct: 395 ARTFAAMKDYQIDGNKEMIALGTMNVVGSMTSCYIATGSFSRSAVNFMAGVETAVSNIVM 454

Query: 440 AVFMALTLLFLAPLFSYTPLVALSAIIMSAMLGLIKYEEMYHIFIVDKFDFCICMAAFLG 499
           A+ +ALTL F+ PLF YTP   L+AII+SA+LGLI  +    I+ +DK DF  CM AFLG
Sbjct: 455 AIVVALTLEFITPLFKYTPNAILAAIIISAVLGLIDIDAAILIWRIDKLDFLACMGAFLG 514

Query: 500 VALLSMDVGIMLSVGLALLRALLYMARPATCKLGKIPNSALYRDMEQYPDSNRVPGIIVL 559
           V  +S+++G++++V ++  + LL + RP T  LGK+PNS +YR+  QYPD+ ++PGI+++
Sbjct: 515 VIFISVEIGLLIAVVISFAKILLQVTRPRTTVLGKLPNSNVYRNTLQYPDAAQIPGILII 574

Query: 560 QLGSPVYYANSNYIRERIFRWIRDEQAMFGSID-GSVQHVLLELSGVTSIDMTGIETLVE 619
           ++ S +Y++NSNY+RER  RW+R+EQ         +++ V++E+S VT ID +GI ++ E
Sbjct: 575 RVDSAIYFSNSNYVRERASRWVREEQENAKEYGMPAIRFVIIEMSPVTDIDTSGIHSIEE 634

Query: 620 IRRSLQAMGIQMGIVNPRIVVMEKMIASEFTDTIGKENIYLSVDDGVERCRDTVVK 674
           + +SL+   IQ+ + NP  VV+EK+ AS+F + IG++NI+L+V D V  C   V +
Sbjct: 635 LLKSLEKQEIQLILANPGPVVIEKLYASKFVEEIGEKNIFLTVGDAVAVCSTEVAE 646

BLAST of Spg022559 vs. TAIR 10
Match: AT1G22150.1 (sulfate transporter 1;3 )

HSP 1 Score: 578.6 bits (1490), Expect = 6.9e-165
Identity = 298/654 (45.57%), Postives = 423/654 (64.68%), Query Frame = 0

Query: 27  KETFFPDDPFRQFQDDRALHRVKKGVQYFIPILDWLPKYNFNMFKYDLLAGITITSLAIP 86
           KETFF DDP R F+D     ++  G+Q   P+++W  KYN  +F+ DL+AG+TI SL IP
Sbjct: 50  KETFFHDDPLRHFKDQSKSKKLMLGIQSVFPVIEWGRKYNLKLFRGDLIAGLTIASLCIP 109

Query: 87  QGISYAKLGSLPPIIGLYSSFVPPLVYAVFGSSKHLAVGTVAACSLLIADIIGAVASPEK 146
           Q I YAKL SL P  GLYSSFVPPLVYA  GSSK +A+G VA  SLL+  ++ A   P  
Sbjct: 110 QDIGYAKLASLDPKYGLYSSFVPPLVYACMGSSKDIAIGPVAVVSLLLGTLLRAEIDPNT 169

Query: 147 EPTLYLHLVYTATFITGVFQAALGFLRLGILVDFLSHSTILGFMGGTAVIICLQQLKGIF 206
            P  YL L +T+TF  GV QAALGF RLG L+DFLSH+ ++GFMGG A+ I LQQLKG  
Sbjct: 170 NPNEYLRLAFTSTFFAGVTQAALGFFRLGFLIDFLSHAAVVGFMGGAAITIALQQLKGFL 229

Query: 207 GLTHFTSKTDVVSVLHAVI-SLRYEWKWQSAVVGVIFLIFLQFTRFLRNRNPKLFWVSAM 266
           G+  FT KTD+++VL +VI S  + W WQ+ ++   FLIFL  ++F+  RN KLFW+ A+
Sbjct: 230 GINKFTKKTDIIAVLSSVISSAHHGWNWQTILISASFLIFLLISKFIGKRNKKLFWIPAI 289

Query: 267 APMVTVIIGCLFAYFIKGEKHGILTAVWDVGIDWVGMESHVIPMFGAPDLSGVGMELWTC 326
           AP+V+VII   F Y  + +K G+                                     
Sbjct: 290 APLVSVIISTFFVYITRADKKGVQ------------------------------------ 349

Query: 327 GFQPNSHILVGHLSKGINPISIKFLNFDSKYISAVLQAGLITGMVALAEGIAIGRSFAIM 386
                   +V HL KG+NP S++ + F   Y+    + G+++GMVAL E +AIGR+FA M
Sbjct: 350 --------IVKHLDKGLNPSSLRLIYFSGDYLLKGFRIGVVSGMVALTEAVAIGRTFAAM 409

Query: 387 KNEQIDGNKEMVAFGLMNIVGSFTSCYLTTGPFSKTAVNFNAGCRTAMSNIVMAVFMALT 446
           K+ QIDGNKEMVA G MN++GS TSCY++TG FS++AVNF AGC+TA+SNI+M++ + LT
Sbjct: 410 KDYQIDGNKEMVALGAMNVIGSMTSCYVSTGSFSRSAVNFMAGCQTAVSNIIMSIVVLLT 469

Query: 447 LLFLAPLFSYTPLVALSAIIMSAMLGLIKYEEMYHIFIVDKFDFCICMAAFLGVALLSMD 506
           LLFL PLF YTP   L+AII++A++ L+       IF +DK DF  CM AF GV  +S++
Sbjct: 470 LLFLTPLFKYTPNAILAAIIINAVIPLVDVNATILIFKIDKLDFVACMGAFFGVIFVSVE 529

Query: 507 VGIMLSVGLALLRALLYMARPATCKLGKIPNSALYRDMEQYPDSNRVPGIIVLQLGSPVY 566
           +G++++VG++  + LL + RP T  LGKIP +++YR++ QYP++ R+PG++ +++ S +Y
Sbjct: 530 IGLLIAVGISFAKILLQVTRPRTAILGKIPGTSVYRNINQYPEATRIPGVLTIRVDSAIY 589

Query: 567 YANSNYIRERIFRWIRDEQAMFGSID-GSVQHVLLELSGVTSIDMTGIETLVEIRRSLQA 626
           ++NSNY+RERI RW+ DE+ M  +     +Q +++E+S VT ID +GI  L ++ +SLQ 
Sbjct: 590 FSNSNYVRERIQRWLTDEEEMVEAARLPRIQFLIIEMSPVTDIDTSGIHALEDLYKSLQK 649

Query: 627 MGIQMGIVNPRIVVMEKMIASEFTDTIGKENIYLSVDDGVERCRDTVVKPKKTD 679
             IQ+ + NP   V+ K+  S F D IG + I+L+V + V+ C      PK +D
Sbjct: 650 RDIQLVLANPGPPVINKLHVSHFADLIGHDKIFLTVAEAVDSC-----SPKLSD 654