Spg022540 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg022540 vs. NCBI nr
Match: XP_022134966.1 (translation initiation factor eIF-2B subunit delta-like isoform X2 [Momordica charantia])

HSP 1 Score: 1187.6 bits (3071), Expect = 0.0e+00
Identity = 618/677 (91.29%), Postives = 636/677 (93.94%), Query Frame = 0

Query: 95  MDARRASRVVIDPKVRQVGFFTPDAPHSPGRTLPAPPDCTTSQLSDSPTSNSLSPVMIPP 154
           MDARR SRVVIDPKVRQVGFFTPDAP SPGRT  APPDCTTSQLSDSPTSNSLSPVMIPP
Sbjct: 1   MDARRGSRVVIDPKVRQVGFFTPDAPPSPGRTTTAPPDCTTSQLSDSPTSNSLSPVMIPP 60

Query: 155 PRHPSDNLASRTAVMPVPASANRRHPEDLVPVGSHNPSETLLGSSPMASPSSRIAGEDLE 214
           PRHPSDNLASRTAV+PVPAS N RHPED VPVGSHNPSETLLGSSP+ASPSSR+ GE+LE
Sbjct: 61  PRHPSDNLASRTAVLPVPASGNWRHPEDQVPVGSHNPSETLLGSSPLASPSSRLGGEELE 120

Query: 215 FSEDSSASVLLRRSGSGKLASSCPGVGFHMAAKPAENLQTMPANSVRTLGTNKPAGATEK 274
           FSEDS+ASVLLRRS SGKLASSCPGVGFHMAAK + NLQTMPANS+RT GTNKPAGATEK
Sbjct: 121 FSEDSAASVLLRRSSSGKLASSCPGVGFHMAAKSSANLQTMPANSMRTSGTNKPAGATEK 180

Query: 275 MGGAPAEVKDHLPSSSKPLKAKTTKAERRALQEAQRAAKISAKGEANKSDTASGATAPGK 334
           +GGA AEVKDH  SSSKPLK KTTKAERRALQEAQRAAK+SAKGEANKS TASG TAP K
Sbjct: 181 IGGASAEVKDHHASSSKPLKTKTTKAERRALQEAQRAAKVSAKGEANKSGTASGTTAPSK 240

Query: 335 AGKQSSTVKGGPATASVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFS 394
            GKQ ST+KGGPATASVASADNRGGDR+VDKERKKDAPPPRMQFDDK+RVE+AKKRAVF+
Sbjct: 241 VGKQPSTIKGGPATASVASADNRGGDRMVDKERKKDAPPPRMQFDDKNRVEKAKKRAVFN 300

Query: 395 QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI 454
           QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI
Sbjct: 301 QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI 360

Query: 455 AMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL 514
           AMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL
Sbjct: 361 AMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL 420

Query: 515 PPSLLESEAKATLCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCVVEMLLS 574
           PP+LLESEAKA LCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSC VEMLLS
Sbjct: 421 PPNLLESEAKAALCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCAVEMLLS 480

Query: 575 YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVNRVFLGAAA 634
           YAH+LGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREV RVFLGAAA
Sbjct: 481 YAHELGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVTRVFLGAAA 540

Query: 635 VLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKVS 694
           VLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKVS
Sbjct: 541 VLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKVS 600

Query: 695 GRTDINYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMVSLSVSLSVCMHMSLLIPP 754
           GRTD+NYLD LT+KEHLQLLNLMYDATPSDYISMIVTDYGM                IPP
Sbjct: 601 GRTDVNYLDGLTAKEHLQLLNLMYDATPSDYISMIVTDYGM----------------IPP 660

Query: 755 TSVPVIVREYRREHLWV 772
           TSVPVIVREYRREHLWV
Sbjct: 661 TSVPVIVREYRREHLWV 661

BLAST of Spg022540 vs. NCBI nr
Match: XP_022134965.1 (translation initiation factor eIF-2B subunit delta-like isoform X1 [Momordica charantia])

HSP 1 Score: 1182.2 bits (3057), Expect = 0.0e+00
Identity = 618/680 (90.88%), Postives = 636/680 (93.53%), Query Frame = 0

Query: 95  MDARRASRVVIDPKVRQVGFFTPDAPHSPGRTLPAPPDCTTSQLSDSPTSNSLSPVMIPP 154
           MDARR SRVVIDPKVRQVGFFTPDAP SPGRT  APPDCTTSQLSDSPTSNSLSPVMIPP
Sbjct: 1   MDARRGSRVVIDPKVRQVGFFTPDAPPSPGRTTTAPPDCTTSQLSDSPTSNSLSPVMIPP 60

Query: 155 PRHPSDNLASRTAVMPVPASANRRHPEDLVPVGSHNPSETLLGSSPMASPSSRIAGEDLE 214
           PRHPSDNLASRTAV+PVPAS N RHPED VPVGSHNPSETLLGSSP+ASPSSR+ GE+LE
Sbjct: 61  PRHPSDNLASRTAVLPVPASGNWRHPEDQVPVGSHNPSETLLGSSPLASPSSRLGGEELE 120

Query: 215 FSEDSSASVLLRRSGSGKLASSCPGVGFHMAAKPAENLQTMPANSVRTLGTNKPAGAT-- 274
           FSEDS+ASVLLRRS SGKLASSCPGVGFHMAAK + NLQTMPANS+RT GTNKPAGAT  
Sbjct: 121 FSEDSAASVLLRRSSSGKLASSCPGVGFHMAAKSSANLQTMPANSMRTSGTNKPAGATAL 180

Query: 275 -EKMGGAPAEVKDHLPSSSKPLKAKTTKAERRALQEAQRAAKISAKGEANKSDTASGATA 334
            EK+GGA AEVKDH  SSSKPLK KTTKAERRALQEAQRAAK+SAKGEANKS TASG TA
Sbjct: 181 AEKIGGASAEVKDHHASSSKPLKTKTTKAERRALQEAQRAAKVSAKGEANKSGTASGTTA 240

Query: 335 PGKAGKQSSTVKGGPATASVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRA 394
           P K GKQ ST+KGGPATASVASADNRGGDR+VDKERKKDAPPPRMQFDDK+RVE+AKKRA
Sbjct: 241 PSKVGKQPSTIKGGPATASVASADNRGGDRMVDKERKKDAPPPRMQFDDKNRVEKAKKRA 300

Query: 395 VFSQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNA 454
           VF+QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNA
Sbjct: 301 VFNQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNA 360

Query: 455 RCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRI 514
           RCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRI
Sbjct: 361 RCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRI 420

Query: 515 AKLPPSLLESEAKATLCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCVVEM 574
           AKLPP+LLESEAKA LCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSC VEM
Sbjct: 421 AKLPPNLLESEAKAALCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCAVEM 480

Query: 575 LLSYAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVNRVFLG 634
           LLSYAH+LGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREV RVFLG
Sbjct: 481 LLSYAHELGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVTRVFLG 540

Query: 635 AAAVLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAIS 694
           AAAVLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAIS
Sbjct: 541 AAAVLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAIS 600

Query: 695 KVSGRTDINYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMVSLSVSLSVCMHMSLL 754
           KVSGRTD+NYLD LT+KEHLQLLNLMYDATPSDYISMIVTDYGM                
Sbjct: 601 KVSGRTDVNYLDGLTAKEHLQLLNLMYDATPSDYISMIVTDYGM---------------- 660

Query: 755 IPPTSVPVIVREYRREHLWV 772
           IPPTSVPVIVREYRREHLWV
Sbjct: 661 IPPTSVPVIVREYRREHLWV 664

BLAST of Spg022540 vs. NCBI nr
Match: KAG7021588.1 (Translation initiation factor eIF-2B subunit delta [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1153.3 bits (2982), Expect = 0.0e+00
Identity = 615/678 (90.71%), Postives = 628/678 (92.63%), Query Frame = 0

Query: 95  MDARRASRVVIDPKVRQVGFFTPDAPHSPGRTLPAPPDCTTSQLSDSPTSNSLSPVMIPP 154
           MDARRASRVVIDPKVRQVGFFTPDAPHSPG  LPA PDCTTS L DSPTSNSLSPVMIPP
Sbjct: 1   MDARRASRVVIDPKVRQVGFFTPDAPHSPGGALPASPDCTTSLLPDSPTSNSLSPVMIPP 60

Query: 155 PRHPSDNLASRTAVMPVPASANRRHPEDLVPVGSHNPSETLLGSSPMASPSSRIAGEDLE 214
           PRHPSDNL+SR  V+PVPAS N RHPEDLV VGSHNPSE LL SSPMAS SSRIAGE+LE
Sbjct: 61  PRHPSDNLSSRLVVLPVPASGNWRHPEDLVRVGSHNPSEMLLRSSPMASHSSRIAGEELE 120

Query: 215 FSEDSSASVLLRRSGSGKLASSCPGVGFHMAAKPAENLQTMPANSVRTLGTNKPAGATEK 274
           FSE SSASVLLRRSGSGKLASSCPGVGF+MAAK  +NL+TMPANSVRTLGTNKPAG TEK
Sbjct: 121 FSEVSSASVLLRRSGSGKLASSCPGVGFYMAAKRGDNLETMPANSVRTLGTNKPAGTTEK 180

Query: 275 MGGAPAEVKDHLPSSSKPLKAKTTKAERRALQEAQRAAKISAKGEANKSDTASGATAPGK 334
            GGA  E KDH PSSSKPLK KTTKAERRALQEAQRAAK+S KGE NKS TASGATA GK
Sbjct: 181 TGGASTEAKDHFPSSSKPLKEKTTKAERRALQEAQRAAKVSGKGEVNKSGTASGATA-GK 240

Query: 335 AGKQSSTVKGGPATASVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFS 394
            GKQ STVK GPAT SVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVF+
Sbjct: 241 VGKQPSTVK-GPATPSVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFN 300

Query: 395 QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI 454
           QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI
Sbjct: 301 QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI 360

Query: 455 AMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL 514
           AMLHAFQ+AIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL
Sbjct: 361 AMLHAFQEAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL 420

Query: 515 PPSLLESEAKATLCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCVVEMLLS 574
           PPSLLESEAKATL LDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGS CVVEMLL 
Sbjct: 421 PPSLLESEAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLL 480

Query: 575 YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVNRVFLGAAA 634
           YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVM EVNRVFLGAAA
Sbjct: 481 YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMGEVNRVFLGAAA 540

Query: 635 VLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKVS 694
           VLANGTVYSRVGTAGIAMVA AFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISK+S
Sbjct: 541 VLANGTVYSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKIS 600

Query: 695 GRTDINYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMVSLSVSLSVCMHMSLLIPP 754
           GR DI+YLDSLTSKE+LQLLNLMYDATPSDYISMIVTDYGM       S+  H  L IPP
Sbjct: 601 GRADISYLDSLTSKENLQLLNLMYDATPSDYISMIVTDYGM-------SLPKHGRLQIPP 660

Query: 755 TSVPVIVREYRREH-LWV 772
           TSVPVIVREYRREH LWV
Sbjct: 661 TSVPVIVREYRREHLLWV 669

BLAST of Spg022540 vs. NCBI nr
Match: XP_023531306.1 (translation initiation factor eIF-2B subunit delta-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1152.5 bits (2980), Expect = 0.0e+00
Identity = 616/678 (90.86%), Postives = 629/678 (92.77%), Query Frame = 0

Query: 95  MDARRASRVVIDPKVRQVGFFTPDAPHSPGRTLPAPPDCTTSQLSDSPTSNSLSPVMIPP 154
           MDARRASRVVIDPKVRQVGFFTPDAPHSPG TLPAPPDCTTS L DSPTSNSLSPVMIPP
Sbjct: 1   MDARRASRVVIDPKVRQVGFFTPDAPHSPGGTLPAPPDCTTSLLPDSPTSNSLSPVMIPP 60

Query: 155 PRHPSDNLASRTAVMPVPASANRRHPEDLVPVGSHNPSETLLGSSPMASPSSRIAGEDLE 214
           PRHPSDNL+SR+AV+      N RHPEDLV VGSHNPSE LL SSPMAS S+RIAGE+LE
Sbjct: 61  PRHPSDNLSSRSAVL-----GNWRHPEDLVRVGSHNPSEMLLRSSPMASSSTRIAGEELE 120

Query: 215 FSEDSSASVLLRRSGSGKLASSCPGVGFHMAAKPAENLQTMPANSVRTLGTNKPAGATEK 274
           FSE SSASVLLRRSGSGKLASSCPGVGF+MAAK  +NL+TMPANSVRTLGTNKPAGATEK
Sbjct: 121 FSEVSSASVLLRRSGSGKLASSCPGVGFYMAAKRGDNLKTMPANSVRTLGTNKPAGATEK 180

Query: 275 MGGAPAEVKDHLPSSSKPLKAKTTKAERRALQEAQRAAKISAKGEANKSDTASGATAPGK 334
           MGGA  E KDHLPSSSKPLK KTTKAERRALQEAQRAAK+SAKGEANKS TASGATA GK
Sbjct: 181 MGGASTEAKDHLPSSSKPLKEKTTKAERRALQEAQRAAKVSAKGEANKSGTASGATA-GK 240

Query: 335 AGKQSSTVKGGPATASVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFS 394
            GKQ STVK GPAT SVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVF+
Sbjct: 241 VGKQPSTVK-GPATPSVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFN 300

Query: 395 QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI 454
           QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI
Sbjct: 301 QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI 360

Query: 455 AMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL 514
           AMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL
Sbjct: 361 AMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL 420

Query: 515 PPSLLESEAKATLCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCVVEMLLS 574
           PPSLLESEAKATL LDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGS CVVEMLL 
Sbjct: 421 PPSLLESEAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLL 480

Query: 575 YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVNRVFLGAAA 634
           YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREV+RVFLGAAA
Sbjct: 481 YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVDRVFLGAAA 540

Query: 635 VLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKVS 694
           VLANGTVYSRVGTAGIAMVA AFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISK+S
Sbjct: 541 VLANGTVYSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKIS 600

Query: 695 GRTDINYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMVSLSVSLSVCMHMSLLIPP 754
           GR DI+YLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGM                IPP
Sbjct: 601 GRADISYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGM----------------IPP 655

Query: 755 TSVPVIVREYRREH-LWV 772
           TSVPVIVREYRREH LWV
Sbjct: 661 TSVPVIVREYRREHLLWV 655

BLAST of Spg022540 vs. NCBI nr
Match: XP_022933250.1 (translation initiation factor eIF-2B subunit delta-like [Cucurbita moschata])

HSP 1 Score: 1150.6 bits (2975), Expect = 0.0e+00
Identity = 612/678 (90.27%), Postives = 625/678 (92.18%), Query Frame = 0

Query: 95  MDARRASRVVIDPKVRQVGFFTPDAPHSPGRTLPAPPDCTTSQLSDSPTSNSLSPVMIPP 154
           MDARRASR+VIDPKVRQVGFFTPDAPHSPG  LPA PDCTTS L DSPTSNSLSPVMIPP
Sbjct: 1   MDARRASRIVIDPKVRQVGFFTPDAPHSPGGALPASPDCTTSLLPDSPTSNSLSPVMIPP 60

Query: 155 PRHPSDNLASRTAVMPVPASANRRHPEDLVPVGSHNPSETLLGSSPMASPSSRIAGEDLE 214
           PRHPSDNL+SR+ V+PVPAS N RHPEDLV VGSHNPSE LL SSPMAS SSRIAGE+LE
Sbjct: 61  PRHPSDNLSSRSVVLPVPASGNWRHPEDLVRVGSHNPSEMLLRSSPMASHSSRIAGEELE 120

Query: 215 FSEDSSASVLLRRSGSGKLASSCPGVGFHMAAKPAENLQTMPANSVRTLGTNKPAGATEK 274
           FSE SSASVLLRRSGSGKLASSCPGVGF+MAAK  +NL+TMPANSVRTLGTNKPAGATEK
Sbjct: 121 FSEVSSASVLLRRSGSGKLASSCPGVGFYMAAKRGDNLETMPANSVRTLGTNKPAGATEK 180

Query: 275 MGGAPAEVKDHLPSSSKPLKAKTTKAERRALQEAQRAAKISAKGEANKSDTASGATAPGK 334
            GGA  E KDH PSSSKPLK KTTKAERRALQEAQR AK+SAKGEANKS TASGATA GK
Sbjct: 181 TGGASTEAKDHFPSSSKPLKEKTTKAERRALQEAQRVAKVSAKGEANKSGTASGATA-GK 240

Query: 335 AGKQSSTVKGGPATASVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFS 394
            GKQ STVK GPAT SVAS DNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVF+
Sbjct: 241 VGKQPSTVK-GPATPSVASTDNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFN 300

Query: 395 QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI 454
           QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI
Sbjct: 301 QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI 360

Query: 455 AMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL 514
           AMLHAFQ+AIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL
Sbjct: 361 AMLHAFQEAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL 420

Query: 515 PPSLLESEAKATLCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCVVEMLLS 574
           PPSLLESEAKATL LDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGS CVVEMLL 
Sbjct: 421 PPSLLESEAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLL 480

Query: 575 YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVNRVFLGAAA 634
           YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVM EVNRVFLGAAA
Sbjct: 481 YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMGEVNRVFLGAAA 540

Query: 635 VLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKVS 694
           VLANGTVYSRVGTAGIAMVA AFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISK+ 
Sbjct: 541 VLANGTVYSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKIF 600

Query: 695 GRTDINYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMVSLSVSLSVCMHMSLLIPP 754
           GR DI+YLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGM                IPP
Sbjct: 601 GRADISYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGM----------------IPP 660

Query: 755 TSVPVIVREYRREH-LWV 772
           TSVPVIVREYRREH LWV
Sbjct: 661 TSVPVIVREYRREHLLWV 660

BLAST of Spg022540 vs. ExPASy Swiss-Prot
Match: P41111 (Translation initiation factor eIF-2B subunit delta OS=Oryctolagus cuniculus OX=9986 GN=EIF2B4 PE=2 SV=2)

HSP 1 Score: 324.3 bits (830), Expect = 3.7e-87
Identity = 203/464 (43.75%), Postives = 278/464 (59.91%), Query Frame = 0

Query: 299 KAERRALQEAQRAAKISAKGEANKSDTASGATAPGKAGKQSSTVKGGPATASVASADNRG 358
           +AERRA QEA+RA K + KGE        G   P          +  P+TA  A A   G
Sbjct: 100 RAERRAKQEAERALKQARKGE-------QGGPPP----------QASPSTAGEAPA---G 159

Query: 359 GDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFSQTEARNRVELFRHLPQYEQGTQLP 418
           G R+ +  +  D   P +    +  V +++++ V ++ +  ++V LF HLPQY +   L 
Sbjct: 160 GKRLTEHTQADD---PTLL---RRLVRKSERQQVPTRKDYGSKVSLFSHLPQYSRQNSLT 219

Query: 419 DLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCIAMLHAFQQAIKDYSTPPQKSLVRD 478
             +S       IHPA+ ++GLQY  G ISG NARCIA+L A QQ I+DY+TPP + L RD
Sbjct: 220 QYMS--IPSSVIHPAMVRLGLQYSQGLISGSNARCIALLRALQQVIQDYTTPPNEELSRD 279

Query: 479 LTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKLPPSLLESEAKATLCLDISRFINEK 538
           L  K+  Y+ F  +CRPLS SM NAI+FL   I  +  +  E EAKA L     R++ EK
Sbjct: 280 LVNKLKPYICFLTQCRPLSASMYNAIKFLNKEITGVSSTKREEEAKAELQAAADRYVQEK 339

Query: 539 IMLADEVIVRHAATKIRDGDVLLTYGSSCVVEMLLSYAHDLGKQFRVVVVDSRPKLEGQA 598
           I+LA + I+R A+ KI +GDV+L YG S +V  +L  A   G++FRVVVVDSRP+LEG+ 
Sbjct: 340 IVLAAQAILRFASKKISNGDVILVYGCSSLVSRILQEAWSEGRKFRVVVVDSRPRLEGRH 399

Query: 599 LLRRLIAKGLSCTYTHINAISYVMREVNRVFLGAAAVLANGTVYSRVGTAGIAMVAHAFH 658
           +LR L+  G+  +Y  I A SYV+ EV++V LGA A+LANG+V SRVGTA +A+VA A +
Sbjct: 400 MLRFLVRAGVPASYLLIPAASYVLPEVSKVLLGAHALLANGSVMSRVGTAQLALVARAHN 459

Query: 659 VPVLFCCEAYKFHERVQLDSICFNELGDPDAISKVSGRTDINYLDSLTSKEHLQLLNLMY 718
           VPVL CCE YKF ERVQ D+   NEL DPD +     R D   L +  S   L+LLNL+Y
Sbjct: 460 VPVLVCCETYKFCERVQTDAFVSNELDDPDDLQ--CERGDHVALANWQSHPSLRLLNLVY 517

Query: 719 DATPSDYISMIVTDYGMVSLSVSLSVCMHMSLLIPPTSVPVIVR 763
           D TP + + +++T+ GM                IP +SVPV++R
Sbjct: 520 DVTPPELVDLVITELGM----------------IPCSSVPVVLR 517

BLAST of Spg022540 vs. ExPASy Swiss-Prot
Match: Q61749 (Translation initiation factor eIF-2B subunit delta OS=Mus musculus OX=10090 GN=Eif2b4 PE=1 SV=2)

HSP 1 Score: 318.5 bits (815), Expect = 2.0e-85
Identity = 208/493 (42.19%), Postives = 285/493 (57.81%), Query Frame = 0

Query: 270 GATEKMGGAPAEVKDHLPSSSKPLKAKTTKAERRALQEAQRAAKISAKGEANKSDTASGA 329
           G   ++GG   E    LP+     KA+  +AERRA QEA+RA K + KGE          
Sbjct: 78  GPGSQLGGTAGE---KLPAGRS--KAE-LRAERRAKQEAERALKQARKGE---QGGVPPQ 137

Query: 330 TAPGKAGKQSSTVKGGPATASVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKK 389
             P  AG+ +S VK  P                        A  P +    +  + +  +
Sbjct: 138 ACPSTAGETTSGVKRVP--------------------EHTPADDPTLL---RRLLRKPDR 197

Query: 390 RAVFSQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGG 449
           + V ++ +  ++V LF HLPQY + + L   +S       IHPA+ ++GLQY  G ISG 
Sbjct: 198 QQVPTRKDYGSKVSLFSHLPQYSRQSSLTQYMS--IPSSVIHPAMVRLGLQYSQGLISGS 257

Query: 450 NARCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKN 509
           NARCIA+LHA QQ I+DY+TPP + L RDL  K+  Y+SF  +CRP+S SM NAI+FL  
Sbjct: 258 NARCIALLHALQQVIQDYTTPPSEELSRDLVNKLKPYISFLTQCRPMSASMCNAIKFLTK 317

Query: 510 RIAKLPPSLLESEAKATLCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCVV 569
            +  +  S  E EAK+ L   + R++ EKI+LA + I R A+TKI DGDV+L YG S +V
Sbjct: 318 EVTGMSSSKREEEAKSELREALDRYVQEKIVLAAQAISRFASTKISDGDVILVYGCSSLV 377

Query: 570 EMLLSYAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVNRVF 629
             +L  A   G++FRVVVVDSRP+LEG+ +L  L+  G+  +Y  I A SYV+ EV++V 
Sbjct: 378 SRILQEARVEGRRFRVVVVDSRPRLEGRHMLHSLVRAGVPTSYLLIPAASYVLPEVSKVL 437

Query: 630 LGAAAVLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDA 689
           LGA A+LANG+V SRVGTA +A+VA A +VPVL CCE YKF ERVQ D+   NEL DPD 
Sbjct: 438 LGAHALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNELDDPDD 497

Query: 690 ISKVSGRTDINYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMVSLSVSLSVCMHMS 749
           +     R D   L +  S   L+LLNL+YD TP + + +++T+ GM              
Sbjct: 498 LQ--CKRGDQVALANWQSHPSLRLLNLVYDVTPPELVDLVITELGM-------------- 518

Query: 750 LLIPPTSVPVIVR 763
             IP +SVPV++R
Sbjct: 558 --IPCSSVPVVLR 518

BLAST of Spg022540 vs. ExPASy Swiss-Prot
Match: Q63186 (Translation initiation factor eIF-2B subunit delta OS=Rattus norvegicus OX=10116 GN=Eif2b4 PE=2 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 3.4e-85
Identity = 221/579 (38.17%), Postives = 300/579 (51.81%), Query Frame = 0

Query: 188 SHNPSETLLGSSPMASPSSRIAGEDLEFSEDSSASVLLRRSGSGKLASSCPGVGFHMAAK 247
           S +  +T L   P A+       E L+  ++       R+   G        V       
Sbjct: 12  SRSEMKTELSPRPGAAGRELTQEEKLQLRKEKKQQKKKRKEEKGADQEIGSAVSAAQRQD 71

Query: 248 PAENLQTMPANSVRTLGTNKPAGATEKMGGAPAEVKDHLPSSSKPLKAKTTKAERRALQE 307
           P   LQ   +    T G   PAG ++      AE+                +AERRA QE
Sbjct: 72  PVRELQGTGSQLGGTTGEKLPAGRSK------AEL----------------RAERRAKQE 131

Query: 308 AQRAAKISAKGEANKSDTASGATAPGKAGKQSSTVKGGPATASVASADNRGGDRIVDKER 367
           A+RA K + KGE       S    P  AG+ +S VK                        
Sbjct: 132 AERALKQARKGE---QGGPSPQACPSTAGEATSGVK------------------------ 191

Query: 368 KKDAPPPRMQFDDKHRVERAKKRAVFSQTEAR----NRVELFRHLPQYEQGTQLPDLVSK 427
                P   Q DD   + R  ++    Q   R    ++V LF HLPQY + + L   +S 
Sbjct: 192 ---RVPEHTQADDPTLLRRLLRKPDRQQVPTRKDYGSKVSLFSHLPQYSRQSSLTQYMS- 251

Query: 428 FFQLDTIHPAIYKVGLQYMAGDISGGNARCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKI 487
                 IHPA+ ++GLQY  G +SG NARCIA+LHA QQ I+DY+TPP + L RDL  K+
Sbjct: 252 -IPSSVIHPAMVRLGLQYSQGLVSGSNARCIALLHALQQVIQDYTTPPNEELSRDLVNKL 311

Query: 488 GVYVSFFNECRPLSVSMGNAIRFLKNRIAKLPPSLLESEAKATLCLDISRFINEKIMLAD 547
             Y+SF  +CRP+S SM NAI+F    +  +  S  E EAK+ L   I R++ EKI+LA 
Sbjct: 312 KPYISFLTQCRPMSASMCNAIKFFNKEVTGMSSSKREEEAKSELKEAIDRYVQEKIVLAS 371

Query: 548 EVIVRHAATKIRDGDVLLTYGSSCVVEMLLSYAHDLGKQFRVVVVDSRPKLEGQALLRRL 607
           + I R A+ KI DGDV+L YG S +V  +L  A   G++FRVVVVDSRP+LEG+ +L  L
Sbjct: 372 QAISRFASKKISDGDVILVYGCSSLVSRILQEAWVEGRRFRVVVVDSRPRLEGRHMLHCL 431

Query: 608 IAKGLSCTYTHINAISYVMREVNRVFLGAAAVLANGTVYSRVGTAGIAMVAHAFHVPVLF 667
           +  G+  +Y  I A SYV+ EV++V LGA A+LANG+V SRVGTA +A+VA A +VPVL 
Sbjct: 432 VRAGVPTSYLLIPAASYVLPEVSKVLLGAHALLANGSVMSRVGTAQLALVARAHNVPVLV 491

Query: 668 CCEAYKFHERVQLDSICFNELGDPDAISKVSGRTDINYLDSLTSKEHLQLLNLMYDATPS 727
           CCE YKF ERVQ D+   NEL DPD +     R D   L +  +   L+LLNL+YD TP 
Sbjct: 492 CCETYKFCERVQTDAFVSNELDDPDDLQ--CKRGDQVTLANWQNNSSLRLLNLVYDVTPP 518

Query: 728 DYISMIVTDYGMVSLSVSLSVCMHMSLLIPPTSVPVIVR 763
           + + +++T+ GM                IP +SVPV++R
Sbjct: 552 ELVDLVITELGM----------------IPCSSVPVVLR 518

BLAST of Spg022540 vs. ExPASy Swiss-Prot
Match: Q3T058 (Translation initiation factor eIF-2B subunit delta OS=Bos taurus OX=9913 GN=EIF2B4 PE=2 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 8.5e-84
Identity = 209/501 (41.72%), Postives = 285/501 (56.89%), Query Frame = 0

Query: 269 AGATEKMGGAPAEVKDHLPSSSKPLKAKTTKAERRALQEAQRAAKISAKGEANKSDTASG 328
           AG   ++G A    K+ +P+     KA+  +AERRA QEA+RA K + KG+         
Sbjct: 78  AGPDSQLGTA----KEKVPAGRS--KAE-LRAERRAKQEAERAMKQARKGD--------- 137

Query: 329 ATAPGKAGKQSSTVKGGP---ATASVASADNRGGDRIVDKERKKDAPPPRMQFDD----K 388
                         +GGP   A  S A     G  R+          P   Q DD    +
Sbjct: 138 --------------QGGPPPQACPSTAGETPSGVKRL----------PEHSQVDDPTLLR 197

Query: 389 HRVERAKKRAVFSQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQY 448
             V++ +++ V ++ +  ++V LF HLPQY +   L   +S       IHPA+ ++GLQY
Sbjct: 198 RLVKKPERQQVPTRKDYGSKVSLFSHLPQYSRQNSLTQFMS--IPSSVIHPAMVRLGLQY 257

Query: 449 MAGDISGGNARCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMG 508
             G +SG NARCIA+L A QQ I+DY+TPP + L RDL  K+  Y SF  +CRPLS SM 
Sbjct: 258 SQGLVSGSNARCIALLRALQQVIQDYTTPPSEELSRDLVNKLKPYFSFLTQCRPLSASMY 317

Query: 509 NAIRFLKNRIAKLPPSLLESEAKATLCLDISRFINEKIMLADEVIVRHAATKIRDGDVLL 568
           NAI+ L   I  +  S  E EAK+ L   I R+I EKI+LA + I R A  KI +GDV+L
Sbjct: 318 NAIKLLNKEITGVSGSKREEEAKSELRAAIDRYIKEKIVLAAQAISRFAYKKISNGDVIL 377

Query: 569 TYGSSCVVEMLLSYAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYV 628
            YG S +V  +L  A   G+QFRVVVVDSRP+LE +  LR L+  G+  +Y  I A SYV
Sbjct: 378 VYGCSSLVSHILQEAWAKGRQFRVVVVDSRPRLEAKHTLRSLVRAGVPASYLLIPAASYV 437

Query: 629 MREVNRVFLGAAAVLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICF 688
           + EV++V LGA A+LANG+V SRVGTA +A+VA A +VPVL CCE YKF ERVQ D+   
Sbjct: 438 LPEVSKVLLGAHALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVS 497

Query: 689 NELGDPDAISKVSGRTDINYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMVSLSVS 748
           NEL DPD +  +  R +   L +  +   L+LLNL+YD TP + + +++T+ GM      
Sbjct: 498 NELDDPDDL--LCERGEHVALANWQNHSSLRLLNLVYDVTPPELVDLVITELGM------ 518

Query: 749 LSVCMHMSLLIPPTSVPVIVR 763
                     IP +SVPV++R
Sbjct: 558 ----------IPCSSVPVVLR 518

BLAST of Spg022540 vs. ExPASy Swiss-Prot
Match: Q9UI10 (Translation initiation factor eIF-2B subunit delta OS=Homo sapiens OX=9606 GN=EIF2B4 PE=1 SV=2)

HSP 1 Score: 311.6 bits (797), Expect = 2.5e-83
Identity = 223/555 (40.18%), Postives = 297/555 (53.51%), Query Frame = 0

Query: 228 SGSGKLASSCPG---VGFHMAAKPAENLQTMPANSVRTLGTNKPAGATEKMGGAPAEVK- 287
           SGSG  A   PG   VG  M  +  E LQ       +     +  GA  + G A +  + 
Sbjct: 12  SGSGMKAELPPGPGAVGREMTKE--EKLQLRKEKKQQKKKRKEEKGAEPETGSAVSAAQC 71

Query: 288 -----DHLPSSSKPLKAKTTK-----------AERRALQEAQRAAKISAKGEANKSDTAS 347
                  LP S   L     K           AERRA QEA+RA K + KGE       +
Sbjct: 72  QVGPTRELPESGIQLGTPREKVPAGRSKAELRAERRAKQEAERALKQARKGEQGGPPPKA 131

Query: 348 GATAPGKAGKQSSTVKGGPATASVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERA 407
              +P  AG+  S VK  P    V   D+    R+V K  ++  P               
Sbjct: 132 ---SPSTAGETPSGVKRLPEYPQV---DDLLLRRLVKKPERQQVP--------------- 191

Query: 408 KKRAVFSQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDIS 467
                 ++ +  ++V LF HLPQY +   L   +S       IHPA+ ++GLQY  G +S
Sbjct: 192 ------TRKDYGSKVSLFSHLPQYSRQNSLTQFMS--IPSSVIHPAMVRLGLQYSQGLVS 251

Query: 468 GGNARCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFL 527
           G NARCIA+L A QQ I+DY+TPP + L RDL  K+  Y+SF  +CRPLS SM NAI+FL
Sbjct: 252 GSNARCIALLRALQQVIQDYTTPPNEELSRDLVNKLKPYMSFLTQCRPLSASMHNAIKFL 311

Query: 528 KNRIAKLPPSLLESEAKATLCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSC 587
              I  +  S  E EAK+ L   I R++ EKI+LA + I R A  KI +GDV+L YG S 
Sbjct: 312 NKEITSVGSSKREEEAKSELRAAIDRYVQEKIVLAAQAISRFAYQKISNGDVILVYGCSS 371

Query: 588 VVEMLLSYAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVNR 647
           +V  +L  A   G++FRVVVVDSRP LEG+  LR L+  G+  +Y  I A SYV+ EV++
Sbjct: 372 LVSRILQEAWTEGRRFRVVVVDSRPWLEGRHTLRSLVHAGVPASYLLIPAASYVLPEVSK 431

Query: 648 VFLGAAAVLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDP 707
           V LGA A+LANG+V SRVGTA +A+VA A +VPVL CCE YKF ERVQ D+   NEL DP
Sbjct: 432 VLLGAHALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNELDDP 491

Query: 708 DAISKVSGRTDINYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMVSLSVSLSVCMH 763
           D +     R +   L +  +   L+LLNL+YD TP + + +++T+ GM            
Sbjct: 492 DDLQ--CKRGEHVALANWQNHASLRLLNLVYDVTPPELVDLVITELGM------------ 517

BLAST of Spg022540 vs. ExPASy TrEMBL
Match: A0A6J1BZ97 (translation initiation factor eIF-2B subunit delta-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111007085 PE=3 SV=1)

HSP 1 Score: 1187.6 bits (3071), Expect = 0.0e+00
Identity = 618/677 (91.29%), Postives = 636/677 (93.94%), Query Frame = 0

Query: 95  MDARRASRVVIDPKVRQVGFFTPDAPHSPGRTLPAPPDCTTSQLSDSPTSNSLSPVMIPP 154
           MDARR SRVVIDPKVRQVGFFTPDAP SPGRT  APPDCTTSQLSDSPTSNSLSPVMIPP
Sbjct: 1   MDARRGSRVVIDPKVRQVGFFTPDAPPSPGRTTTAPPDCTTSQLSDSPTSNSLSPVMIPP 60

Query: 155 PRHPSDNLASRTAVMPVPASANRRHPEDLVPVGSHNPSETLLGSSPMASPSSRIAGEDLE 214
           PRHPSDNLASRTAV+PVPAS N RHPED VPVGSHNPSETLLGSSP+ASPSSR+ GE+LE
Sbjct: 61  PRHPSDNLASRTAVLPVPASGNWRHPEDQVPVGSHNPSETLLGSSPLASPSSRLGGEELE 120

Query: 215 FSEDSSASVLLRRSGSGKLASSCPGVGFHMAAKPAENLQTMPANSVRTLGTNKPAGATEK 274
           FSEDS+ASVLLRRS SGKLASSCPGVGFHMAAK + NLQTMPANS+RT GTNKPAGATEK
Sbjct: 121 FSEDSAASVLLRRSSSGKLASSCPGVGFHMAAKSSANLQTMPANSMRTSGTNKPAGATEK 180

Query: 275 MGGAPAEVKDHLPSSSKPLKAKTTKAERRALQEAQRAAKISAKGEANKSDTASGATAPGK 334
           +GGA AEVKDH  SSSKPLK KTTKAERRALQEAQRAAK+SAKGEANKS TASG TAP K
Sbjct: 181 IGGASAEVKDHHASSSKPLKTKTTKAERRALQEAQRAAKVSAKGEANKSGTASGTTAPSK 240

Query: 335 AGKQSSTVKGGPATASVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFS 394
            GKQ ST+KGGPATASVASADNRGGDR+VDKERKKDAPPPRMQFDDK+RVE+AKKRAVF+
Sbjct: 241 VGKQPSTIKGGPATASVASADNRGGDRMVDKERKKDAPPPRMQFDDKNRVEKAKKRAVFN 300

Query: 395 QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI 454
           QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI
Sbjct: 301 QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI 360

Query: 455 AMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL 514
           AMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL
Sbjct: 361 AMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL 420

Query: 515 PPSLLESEAKATLCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCVVEMLLS 574
           PP+LLESEAKA LCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSC VEMLLS
Sbjct: 421 PPNLLESEAKAALCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCAVEMLLS 480

Query: 575 YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVNRVFLGAAA 634
           YAH+LGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREV RVFLGAAA
Sbjct: 481 YAHELGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVTRVFLGAAA 540

Query: 635 VLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKVS 694
           VLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKVS
Sbjct: 541 VLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKVS 600

Query: 695 GRTDINYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMVSLSVSLSVCMHMSLLIPP 754
           GRTD+NYLD LT+KEHLQLLNLMYDATPSDYISMIVTDYGM                IPP
Sbjct: 601 GRTDVNYLDGLTAKEHLQLLNLMYDATPSDYISMIVTDYGM----------------IPP 660

Query: 755 TSVPVIVREYRREHLWV 772
           TSVPVIVREYRREHLWV
Sbjct: 661 TSVPVIVREYRREHLWV 661

BLAST of Spg022540 vs. ExPASy TrEMBL
Match: A0A6J1BZT6 (translation initiation factor eIF-2B subunit delta-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007085 PE=3 SV=1)

HSP 1 Score: 1182.2 bits (3057), Expect = 0.0e+00
Identity = 618/680 (90.88%), Postives = 636/680 (93.53%), Query Frame = 0

Query: 95  MDARRASRVVIDPKVRQVGFFTPDAPHSPGRTLPAPPDCTTSQLSDSPTSNSLSPVMIPP 154
           MDARR SRVVIDPKVRQVGFFTPDAP SPGRT  APPDCTTSQLSDSPTSNSLSPVMIPP
Sbjct: 1   MDARRGSRVVIDPKVRQVGFFTPDAPPSPGRTTTAPPDCTTSQLSDSPTSNSLSPVMIPP 60

Query: 155 PRHPSDNLASRTAVMPVPASANRRHPEDLVPVGSHNPSETLLGSSPMASPSSRIAGEDLE 214
           PRHPSDNLASRTAV+PVPAS N RHPED VPVGSHNPSETLLGSSP+ASPSSR+ GE+LE
Sbjct: 61  PRHPSDNLASRTAVLPVPASGNWRHPEDQVPVGSHNPSETLLGSSPLASPSSRLGGEELE 120

Query: 215 FSEDSSASVLLRRSGSGKLASSCPGVGFHMAAKPAENLQTMPANSVRTLGTNKPAGAT-- 274
           FSEDS+ASVLLRRS SGKLASSCPGVGFHMAAK + NLQTMPANS+RT GTNKPAGAT  
Sbjct: 121 FSEDSAASVLLRRSSSGKLASSCPGVGFHMAAKSSANLQTMPANSMRTSGTNKPAGATAL 180

Query: 275 -EKMGGAPAEVKDHLPSSSKPLKAKTTKAERRALQEAQRAAKISAKGEANKSDTASGATA 334
            EK+GGA AEVKDH  SSSKPLK KTTKAERRALQEAQRAAK+SAKGEANKS TASG TA
Sbjct: 181 AEKIGGASAEVKDHHASSSKPLKTKTTKAERRALQEAQRAAKVSAKGEANKSGTASGTTA 240

Query: 335 PGKAGKQSSTVKGGPATASVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRA 394
           P K GKQ ST+KGGPATASVASADNRGGDR+VDKERKKDAPPPRMQFDDK+RVE+AKKRA
Sbjct: 241 PSKVGKQPSTIKGGPATASVASADNRGGDRMVDKERKKDAPPPRMQFDDKNRVEKAKKRA 300

Query: 395 VFSQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNA 454
           VF+QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNA
Sbjct: 301 VFNQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNA 360

Query: 455 RCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRI 514
           RCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRI
Sbjct: 361 RCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRI 420

Query: 515 AKLPPSLLESEAKATLCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCVVEM 574
           AKLPP+LLESEAKA LCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSC VEM
Sbjct: 421 AKLPPNLLESEAKAALCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCAVEM 480

Query: 575 LLSYAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVNRVFLG 634
           LLSYAH+LGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREV RVFLG
Sbjct: 481 LLSYAHELGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVTRVFLG 540

Query: 635 AAAVLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAIS 694
           AAAVLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAIS
Sbjct: 541 AAAVLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAIS 600

Query: 695 KVSGRTDINYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMVSLSVSLSVCMHMSLL 754
           KVSGRTD+NYLD LT+KEHLQLLNLMYDATPSDYISMIVTDYGM                
Sbjct: 601 KVSGRTDVNYLDGLTAKEHLQLLNLMYDATPSDYISMIVTDYGM---------------- 660

Query: 755 IPPTSVPVIVREYRREHLWV 772
           IPPTSVPVIVREYRREHLWV
Sbjct: 661 IPPTSVPVIVREYRREHLWV 664

BLAST of Spg022540 vs. ExPASy TrEMBL
Match: A0A6J1F4E1 (translation initiation factor eIF-2B subunit delta-like OS=Cucurbita moschata OX=3662 GN=LOC111440216 PE=3 SV=1)

HSP 1 Score: 1150.6 bits (2975), Expect = 0.0e+00
Identity = 612/678 (90.27%), Postives = 625/678 (92.18%), Query Frame = 0

Query: 95  MDARRASRVVIDPKVRQVGFFTPDAPHSPGRTLPAPPDCTTSQLSDSPTSNSLSPVMIPP 154
           MDARRASR+VIDPKVRQVGFFTPDAPHSPG  LPA PDCTTS L DSPTSNSLSPVMIPP
Sbjct: 1   MDARRASRIVIDPKVRQVGFFTPDAPHSPGGALPASPDCTTSLLPDSPTSNSLSPVMIPP 60

Query: 155 PRHPSDNLASRTAVMPVPASANRRHPEDLVPVGSHNPSETLLGSSPMASPSSRIAGEDLE 214
           PRHPSDNL+SR+ V+PVPAS N RHPEDLV VGSHNPSE LL SSPMAS SSRIAGE+LE
Sbjct: 61  PRHPSDNLSSRSVVLPVPASGNWRHPEDLVRVGSHNPSEMLLRSSPMASHSSRIAGEELE 120

Query: 215 FSEDSSASVLLRRSGSGKLASSCPGVGFHMAAKPAENLQTMPANSVRTLGTNKPAGATEK 274
           FSE SSASVLLRRSGSGKLASSCPGVGF+MAAK  +NL+TMPANSVRTLGTNKPAGATEK
Sbjct: 121 FSEVSSASVLLRRSGSGKLASSCPGVGFYMAAKRGDNLETMPANSVRTLGTNKPAGATEK 180

Query: 275 MGGAPAEVKDHLPSSSKPLKAKTTKAERRALQEAQRAAKISAKGEANKSDTASGATAPGK 334
            GGA  E KDH PSSSKPLK KTTKAERRALQEAQR AK+SAKGEANKS TASGATA GK
Sbjct: 181 TGGASTEAKDHFPSSSKPLKEKTTKAERRALQEAQRVAKVSAKGEANKSGTASGATA-GK 240

Query: 335 AGKQSSTVKGGPATASVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFS 394
            GKQ STVK GPAT SVAS DNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVF+
Sbjct: 241 VGKQPSTVK-GPATPSVASTDNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFN 300

Query: 395 QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI 454
           QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI
Sbjct: 301 QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI 360

Query: 455 AMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL 514
           AMLHAFQ+AIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL
Sbjct: 361 AMLHAFQEAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL 420

Query: 515 PPSLLESEAKATLCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCVVEMLLS 574
           PPSLLESEAKATL LDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGS CVVEMLL 
Sbjct: 421 PPSLLESEAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLL 480

Query: 575 YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVNRVFLGAAA 634
           YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVM EVNRVFLGAAA
Sbjct: 481 YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMGEVNRVFLGAAA 540

Query: 635 VLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKVS 694
           VLANGTVYSRVGTAGIAMVA AFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISK+ 
Sbjct: 541 VLANGTVYSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKIF 600

Query: 695 GRTDINYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMVSLSVSLSVCMHMSLLIPP 754
           GR DI+YLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGM                IPP
Sbjct: 601 GRADISYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGM----------------IPP 660

Query: 755 TSVPVIVREYRREH-LWV 772
           TSVPVIVREYRREH LWV
Sbjct: 661 TSVPVIVREYRREHLLWV 660

BLAST of Spg022540 vs. ExPASy TrEMBL
Match: A0A6J1KJK1 (translation initiation factor eIF-2B subunit delta-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495813 PE=3 SV=1)

HSP 1 Score: 1130.9 bits (2924), Expect = 0.0e+00
Identity = 606/678 (89.38%), Postives = 620/678 (91.45%), Query Frame = 0

Query: 95  MDARRASRVVIDPKVRQVGFFTPDAPHSPGRTLPAPPDCTTSQLSDSPTSNSLSPVMIPP 154
           MDARRASRVVIDPKVRQVGFFTPDAPHSPG  LP PPDCTTS L DSPTSNSLSPVMIPP
Sbjct: 1   MDARRASRVVIDPKVRQVGFFTPDAPHSPGGALPTPPDCTTSLLPDSPTSNSLSPVMIPP 60

Query: 155 PRHPSDNLASRTAVMPVPASANRRHPEDLVPVGSHNPSETLLGSSPMASPSSRIAGEDLE 214
           PRHPSDNL+SR+A        N RHPEDLV VGSHNPSE LL SSPMASPS+RIAGE+LE
Sbjct: 61  PRHPSDNLSSRSA-------GNWRHPEDLVRVGSHNPSEMLLRSSPMASPSTRIAGEELE 120

Query: 215 FSEDSSASVLLRRSGSGKLASSCPGVGFHMAAKPAENLQTMPANSVRTLGTNKPAGATEK 274
            SE SSASVLLRRSGSGKLASSCPGVGF+MAAK  +NL+TMPANSVRTLGTNKPA ATEK
Sbjct: 121 SSEVSSASVLLRRSGSGKLASSCPGVGFYMAAKRGDNLETMPANSVRTLGTNKPAEATEK 180

Query: 275 MGGAPAEVKDHLPSSSKPLKAKTTKAERRALQEAQRAAKISAKGEANKSDTASGATAPGK 334
           MGGA  E KDHLPSSSKPLK KTTKAERRALQEAQRAAK+SAKGEANKS +ASGATA GK
Sbjct: 181 MGGASTEAKDHLPSSSKPLKEKTTKAERRALQEAQRAAKVSAKGEANKSGSASGATA-GK 240

Query: 335 AGKQSSTVKGGPATASVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFS 394
            GKQ STVK GPAT SVASADNRGGDRIVDKERKKDAPPPRMQFDDK+RVERAKKRAVF+
Sbjct: 241 VGKQPSTVK-GPATPSVASADNRGGDRIVDKERKKDAPPPRMQFDDKNRVERAKKRAVFN 300

Query: 395 QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI 454
           QTEARNRV LFRHLPQYEQGTQLPDLVSKFFQLDT HPAIYKVGLQYMAGDISGGNARCI
Sbjct: 301 QTEARNRVALFRHLPQYEQGTQLPDLVSKFFQLDTFHPAIYKVGLQYMAGDISGGNARCI 360

Query: 455 AMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL 514
           AMLHAFQ+AIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL
Sbjct: 361 AMLHAFQEAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL 420

Query: 515 PPSLLESEAKATLCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCVVEMLLS 574
           PPSLLESEAKATL LDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGS CVVEMLL 
Sbjct: 421 PPSLLESEAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLL 480

Query: 575 YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVNRVFLGAAA 634
           YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVNRVFLGAAA
Sbjct: 481 YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVNRVFLGAAA 540

Query: 635 VLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKVS 694
           VLANGTVYSRVGTAGIAMVA AFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISK+S
Sbjct: 541 VLANGTVYSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKIS 600

Query: 695 GRTDINYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMVSLSVSLSVCMHMSLLIPP 754
           GR DI+YLDSLTSKEH QLLNLMYDATPSDYISMIVTDYGM                IP 
Sbjct: 601 GRADISYLDSLTSKEHFQLLNLMYDATPSDYISMIVTDYGM----------------IPA 653

Query: 755 TSVPVIVREYRREH-LWV 772
           TSVPVIVREYRREH LWV
Sbjct: 661 TSVPVIVREYRREHLLWV 653

BLAST of Spg022540 vs. ExPASy TrEMBL
Match: A0A6J1BZS4 (translation initiation factor eIF-2B subunit delta-like OS=Momordica charantia OX=3673 GN=LOC111007076 PE=3 SV=1)

HSP 1 Score: 1010.7 bits (2612), Expect = 3.1e-291
Identity = 540/678 (79.65%), Postives = 582/678 (85.84%), Query Frame = 0

Query: 95  MDARRASRVVIDPKVRQVGFFTPDAPHSPGRTLPAPPDCTTSQLSDSPTSNSLSPVMIPP 154
           MDARR SR+V +PKVRQVGFFT DAP+ P R+L  P DCTTS  S SP +NSLSPVMIPP
Sbjct: 1   MDARRGSRIV-NPKVRQVGFFTIDAPNPPSRSLSVPADCTTSPQSHSPLTNSLSPVMIPP 60

Query: 155 PRHPSDNLASRTAVMPVPASANRRHPEDLVPVGSHNPSETLLGSSPMASPSSRIAGEDLE 214
           PRH SDNLASR+ V+PVPA AN R+  D +PVGS+NPSETLLG+SP+ASPSSRI  E+L+
Sbjct: 61  PRHLSDNLASRSTVLPVPAPANWRNSADQIPVGSYNPSETLLGTSPVASPSSRIEDEELD 120

Query: 215 FSEDSSASVLLRRSGSGKLASSCPGVGFHMAAKPAENLQTMPANSVRTLGTNKPAGATEK 274
            SEDS+ SV  RRSGSGK+ASS PGVG H+AAK +EN Q +P  + RT   +KP G  EK
Sbjct: 121 LSEDSATSVWYRRSGSGKIASSFPGVGIHVAAKSSENSQAVPLANARTSEPDKPTGVAEK 180

Query: 275 MGGAPAEV-KDHLPSSSKPLKAKTTKAERRALQEAQRAAKISAKGEANKSDTASGATAPG 334
            GGA  EV    LP SSKPLKAKTTKAERRALQEAQRAAK+SAKGEANKS     +TAPG
Sbjct: 181 NGGASTEVPNQQLPPSSKPLKAKTTKAERRALQEAQRAAKVSAKGEANKS-----STAPG 240

Query: 335 KAGKQSSTVKGGPATASVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVF 394
           KA KQ S +K  PA  SVAS D RGGDR +DKERKKDAP PRMQFDDK+RVE+AKKRAVF
Sbjct: 241 KAVKQPSAIKDSPAAPSVASTDKRGGDRTLDKERKKDAPHPRMQFDDKNRVEKAKKRAVF 300

Query: 395 SQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARC 454
           +QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDI GGNARC
Sbjct: 301 NQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDICGGNARC 360

Query: 455 IAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAK 514
           IAMLHAFQQAIKDYSTPPQKSL RDLTAKIG YVSFFNECRPLSVSMGNAIRFLKNRIAK
Sbjct: 361 IAMLHAFQQAIKDYSTPPQKSLGRDLTAKIGSYVSFFNECRPLSVSMGNAIRFLKNRIAK 420

Query: 515 LPPSLLESEAKATLCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCVVEMLL 574
           LP +L ESE KATLC DI+RFINEKI+LADEVIVRHAATKIRDGDVLLTYGSS  VEMLL
Sbjct: 421 LPLNLPESEVKATLCSDINRFINEKIILADEVIVRHAATKIRDGDVLLTYGSSSAVEMLL 480

Query: 575 SYAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVNRVFLGAA 634
            YAH+LGKQFRVVVVDSRPK+EGQALLRRLI +GLSCTYTHINAISYVM EV RVFLGAA
Sbjct: 481 LYAHELGKQFRVVVVDSRPKMEGQALLRRLITRGLSCTYTHINAISYVMHEVTRVFLGAA 540

Query: 635 AVLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKV 694
           AVL+NGTVYSRVGTAG+AMVA+AFHVPVL CCEAYKFHERVQLDSICFNELGDPDAISKV
Sbjct: 541 AVLSNGTVYSRVGTAGVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDAISKV 600

Query: 695 SGRTDINYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMVSLSVSLSVCMHMSLLIP 754
            GR +INYLD+LT+KE LQLLNLMYDATPSDYISMIVTDYGM                IP
Sbjct: 601 PGREEINYLDNLTAKEQLQLLNLMYDATPSDYISMIVTDYGM----------------IP 656

Query: 755 PTSVPVIVREYRREHLWV 772
           PTSVPVIVREYRREH+W+
Sbjct: 661 PTSVPVIVREYRREHVWI 656

BLAST of Spg022540 vs. TAIR 10
Match: AT5G38640.1 (NagB/RpiA/CoA transferase-like superfamily protein )

HSP 1 Score: 696.8 bits (1797), Expect = 1.9e-200
Identity = 408/693 (58.87%), Postives = 485/693 (69.99%), Query Frame = 0

Query: 95  MDARRASRVVIDPKVRQVGFFTPDAPHSPGRTLPAPPDCTTSQLSD----SPTSNSLSPV 154
           M++RR  R V+DPKVRQVGFFT   P S  +     P   TS  S     SP+ NSLSPV
Sbjct: 1   MESRRNPRAVVDPKVRQVGFFTSSQPDSSTQQQQPDPIAATSASSSSSTISPSGNSLSPV 60

Query: 155 MIPPPR-HPSDNLASRTAVMPVPASANRRHPEDLVPVGSHNPSETLLGSSPMASPSSRIA 214
           MIPPPR + SD    R AV    ASA                           SPSS   
Sbjct: 61  MIPPPRLNSSDTFPLRVAVAGAAASA--------------------------VSPSSFSY 120

Query: 215 GEDLEFSEDSSASVLLRRS------GSGKLASSCPGVGFHMAAKPAENLQTMPANSVRTL 274
               +F + S+ +   R S       S  +ASS PG+G  M +  A    ++PA+ + T+
Sbjct: 121 SSRRDFPDGSTTASPGRHSRGSFVKSSVPVASSLPGIG--MDSMAAAKSSSVPASGLTTV 180

Query: 275 G-TNKPAGATEKMGGAPAEVKDHLPSSSKPLKAKTTKAERRALQEAQRAAKISAKGEANK 334
                P G +EK  G   +         KPLK KTTKAERRA+QEAQRAAK +AK +  K
Sbjct: 181 SVVTMPPGLSEKASGMEVQ-----SDQKKPLKEKTTKAERRAIQEAQRAAKAAAKADGGK 240

Query: 335 ---SDTASGATAPGKAGKQS-STVKGGPATASVASADNRGGDRIVDKERKKDAPPPRMQF 394
                +AS A+   KA K + +T +      +  +A+ +GG    +K+RKKDAP PRMQ+
Sbjct: 241 VAPVSSASVASTSVKAAKPAKATSQKNDVAVATGAAEKKGGPS--EKDRKKDAPHPRMQY 300

Query: 395 DDKHRVERAKKRAVFSQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVG 454
           DD+ RV +AK+RAV  QTEA+NRVELFRHLPQYE GTQLPDL +KFFQLD +HPA+YKVG
Sbjct: 301 DDESRVVKAKRRAVVKQTEAKNRVELFRHLPQYEHGTQLPDLETKFFQLDPMHPAVYKVG 360

Query: 455 LQYMAGDISGGNARCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSV 514
           LQY++GDISGGNARCIAML AFQ+ +KDYSTPP+KSL RD+TAKI  YVSF  ECRPLS+
Sbjct: 361 LQYLSGDISGGNARCIAMLQAFQEVVKDYSTPPEKSLNRDMTAKISSYVSFLIECRPLSI 420

Query: 515 SMGNAIRFLKNRIAKLPPSLLESEAKATLCLDISRFINEKIMLADEVIVRHAATKIRDGD 574
           SMGNAIRF+KNRIAKLP +L ESEAKATL  DI RFINEKI++AD VIV+HA TKIRDGD
Sbjct: 421 SMGNAIRFVKNRIAKLPITLSESEAKATLQSDIERFINEKIIVADTVIVKHAVTKIRDGD 480

Query: 575 VLLTYGSSCVVEMLLSYAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAI 634
           VLLTYGS   VEM++ +AH+L K+FRV+VVDSRPKLEGQ LLRRLI +G++CTYTHINAI
Sbjct: 481 VLLTYGSPTAVEMVILHAHELRKKFRVLVVDSRPKLEGQLLLRRLIKRGINCTYTHINAI 540

Query: 635 SYVMREVNRVFLGAAAVLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDS 694
           SY+M EV +VFLGA++VL+NGTVYSRVGTA +AMVA+AF VPVL CCEAYKFHERVQLDS
Sbjct: 541 SYIMHEVTKVFLGASSVLSNGTVYSRVGTACVAMVANAFRVPVLVCCEAYKFHERVQLDS 600

Query: 695 ICFNELGDPDAISKVSGRTDINYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMVSL 754
           IC NELGDP+AIS+V GR DINYLD LT+  +LQ LNLMYDATPSDYISMI+TDYGMV  
Sbjct: 601 ICSNELGDPNAISEVHGREDINYLDGLTNNANLQFLNLMYDATPSDYISMIITDYGMV-- 642

Query: 755 SVSLSVCMHMSLLIPPTSVPVIVREYRREHLWV 772
                         PPTSVPVIVREY++EHL V
Sbjct: 661 --------------PPTSVPVIVREYQKEHLLV 642

BLAST of Spg022540 vs. TAIR 10
Match: AT1G48970.1 (NagB/RpiA/CoA transferase-like superfamily protein )

HSP 1 Score: 670.6 bits (1729), Expect = 1.5e-192
Identity = 396/696 (56.90%), Postives = 491/696 (70.55%), Query Frame = 0

Query: 95  MDARRASRVVIDPKVRQVGFFTPDAP------HSPGRTLPAPPDCTTSQ--LSDSPTSNS 154
           MD RR +  V  PKVR+VGFFT   P        P R+   P D TTS   LSDSP+ N 
Sbjct: 1   MDTRRGALAV--PKVRRVGFFTSIEPPPESSLQRPNRSQSGPVDATTSSSPLSDSPSGNL 60

Query: 155 LSPVMIPPPRHPSDNLASRTAV-----MPVPA-SANRRH--PE-DLVPVGSHNPSETLLG 214
           +SPV+IPP RH SDNL SR A      +PVP  +A RR+  PE  L+ VGS+NP ++LLG
Sbjct: 61  ISPVLIPPSRHHSDNLTSRVAAAAAAPVPVPGPAAFRRYLTPERTLLHVGSYNPPDSLLG 120

Query: 215 SSPMASPSSRIAGEDLEFSEDSSASVLLRRSGSGKLASSCPGVGFH--MAAKPAENLQTM 274
           +S   SPSS         SEDS++    +RS S KL++S P  GF   +A +  +  +T 
Sbjct: 121 TS---SPSSNGG-----LSEDSASLFGFQRSDSTKLSASLPNGGFDLTLAVRAPQESETK 180

Query: 275 PANSVRTLGTNKPAGATEKMGGAPAEVKDHLPSSSKPLKAKTTKAERRALQEAQRAAKIS 334
            A +  + G  K A  + K     A  K       K LK KTTKAERRA+QEAQRAAK +
Sbjct: 181 IATTSASQGKKKIAEVSGK--SESATTKPQKEKEPKALKDKTTKAERRAIQEAQRAAKAA 240

Query: 335 AKGEANKSDTASGATAPGKAG-KQSSTVKGGPATASVASADNRGGDRIVDKERKKDAPPP 394
           AKGE ++    SG   PGKA  K     +  P T+SV+          V+KE++ D P  
Sbjct: 241 AKGEGSRRADESGRANPGKAAIKPQPKKERLPVTSSVSEKTAVA----VEKEKRMDVPQT 300

Query: 395 RMQFDDKHRVERAKKRAVFSQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAI 454
           +MQ+DDK RV++AK+RAV  QTE++N+VELF HLPQYE+  QLP+L S FF LD+IH A+
Sbjct: 301 QMQYDDKSRVDKAKRRAVVEQTESKNKVELFLHLPQYERSNQLPNLSSNFFTLDSIHHAV 360

Query: 455 YKVGLQYMAGDISGGNARCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECR 514
           YKVGLQ++AGDISG NARCIAML AFQ+AI+DYSTPP K L  DLTAKI  YVSF  ECR
Sbjct: 361 YKVGLQHLAGDISGDNARCIAMLQAFQEAIEDYSTPPMKDLTMDLTAKINGYVSFLIECR 420

Query: 515 PLSVSMGNAIRFLKNRIAKLPPSLLESEAKATLCLDISRFINEKIMLADEVIVRHAATKI 574
           PLS+SMGNAIRFLKN+I KLP +L ESEAK++LC DI RFI+EK ++AD+ IV+HA TKI
Sbjct: 421 PLSMSMGNAIRFLKNQIRKLPVNLSESEAKSSLCSDIGRFIDEKTIIADKAIVQHAVTKI 480

Query: 575 RDGDVLLTYGSSCVVEMLLSYAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTH 634
           RDG+VLLTYG SCVVEM+L YAH++GK+FRVV+VDSRP LEGQ LLRRL+ +GL CTYTH
Sbjct: 481 RDGEVLLTYGFSCVVEMILLYAHEIGKKFRVVIVDSRPNLEGQKLLRRLVTRGLDCTYTH 540

Query: 635 INAISYVMREVNRVFLGAAAVLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERV 694
           INAISY+MRE  RVFLGA+++ +NGT+Y+RVGT+ IAMVA+AF VPV+ CCEAYKFHERV
Sbjct: 541 INAISYIMREATRVFLGASSIYSNGTLYARVGTSCIAMVANAFSVPVIVCCEAYKFHERV 600

Query: 695 QLDSICFNELGDPDAISKV-SGRTDINYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDY 754
            LDSIC NELGDPDA++ + S RT+  +  ++ + ++LQ LNLMYD+TPS+YISMIV+DY
Sbjct: 601 LLDSICSNELGDPDAVANIPSFRTNAKHSKTMDNNKNLQFLNLMYDSTPSEYISMIVSDY 660

Query: 755 GMVSLSVSLSVCMHMSLLIPPTSVPVIVREYRREHL 770
           GM                IPPTS+PVIVREYRRE+L
Sbjct: 661 GM----------------IPPTSIPVIVREYRRENL 664

BLAST of Spg022540 vs. TAIR 10
Match: AT2G44070.1 (NagB/RpiA/CoA transferase-like superfamily protein )

HSP 1 Score: 481.9 bits (1239), Expect = 9.8e-136
Identity = 243/341 (71.26%), Postives = 284/341 (83.28%), Query Frame = 0

Query: 430 IHPAIYKVGLQYMAGDISGGNARCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSF 489
           +H A+YKVGL Y++ DISGGNARCIAML AFQ+ +KDYSTPP+ +L RD+TA+I  YVSF
Sbjct: 1   MHSAVYKVGLHYLSWDISGGNARCIAMLQAFQEVVKDYSTPPENTLNRDMTAQISSYVSF 60

Query: 490 FNECRPLSVSMGNAIRFLKNRIAKLPPSLLESEAKATLCLDISRFINEKIMLADEVIVRH 549
             ECRPLS+SMGNAIRF+KNRIAKLP +L ESEAKA+L  DI RFINEKI+ AD VIV H
Sbjct: 61  LIECRPLSISMGNAIRFVKNRIAKLPITLSESEAKASLHSDIERFINEKIIGADTVIVEH 120

Query: 550 AATKIRDGDVLLTYGSSCVVEMLLSYAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLS 609
           A TKIRDGDVLLTYGSS V+EM+L +AH+LGK+FRV VVDSRPKL+G+ LLRRLI +G++
Sbjct: 121 AVTKIRDGDVLLTYGSSTVIEMILVHAHELGKKFRVFVVDSRPKLQGKLLLRRLIKRGIN 180

Query: 610 CTYTHINAISYVMREVNRVFLGAAAVLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYK 669
           CTYTHI AISY+M +V +VFLGA++V +NGTVYSRVGTA +AMVA+AF VPVL CCEAYK
Sbjct: 181 CTYTHITAISYIMHQVTKVFLGASSVFSNGTVYSRVGTACVAMVANAFRVPVLVCCEAYK 240

Query: 670 FHERVQLD-SICFNELGDPDAISKVSGRTDINYLDSLTSKEHLQLLNLMYDATPSDYISM 729
           FHE+VQLD SIC NELGDP+AISKV GR DINYL+ LT+   L+ LNLMYDATPSDYISM
Sbjct: 241 FHEKVQLDYSICSNELGDPNAISKVHGREDINYLNGLTNNAKLKFLNLMYDATPSDYISM 300

Query: 730 IVTDYGMVSLSVSLSVCMHMSLLIPPTSVPVIVREYRREHL 770
           I+TDYGMV                PPTSVPVIVREY++EHL
Sbjct: 301 IITDYGMV----------------PPTSVPVIVREYQKEHL 325

BLAST of Spg022540 vs. TAIR 10
Match: AT3G07300.1 (NagB/RpiA/CoA transferase-like superfamily protein )

HSP 1 Score: 87.8 bits (216), Expect = 4.1e-17
Identity = 66/219 (30.14%), Postives = 102/219 (46.58%), Query Frame = 0

Query: 517 SLLESEAKATLCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCVVEMLLSYA 576
           S L  + K  +   +++ I E I    E I   A   I   +V+LT GSS  V   L  A
Sbjct: 170 SSLTRKLKHDVIEGVNQLIQE-IAGCHEQIAEQAIEHIHQNEVILTLGSSRTVLEFLCAA 229

Query: 577 HDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVNRVFLGAAAVL 636
            +  + FRV V +  P+ +G  L + L+A+GL  T    +A+  ++  VN V +GA AV+
Sbjct: 230 KEKKRSFRVFVAEGAPRYQGHLLAKELVARGLQTTVITDSAVFAMISRVNMVIIGAHAVM 289

Query: 637 ANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLD-SICFNELGDPDAISKVSG 696
           ANG V   VG    A+ A    VP +    ++K       +  +  NEL  P  +     
Sbjct: 290 ANGGVIGPVGVNMAALAAQKHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGE 349

Query: 697 RTDINYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYG 735
            +D   LD       LQ++N  +D  P + +S+ +TD G
Sbjct: 350 FSDC--LDFGAGSGSLQVVNPTFDYVPPNLVSLFITDTG 385

BLAST of Spg022540 vs. TAIR 10
Match: AT3G07300.2 (NagB/RpiA/CoA transferase-like superfamily protein )

HSP 1 Score: 87.8 bits (216), Expect = 4.1e-17
Identity = 66/219 (30.14%), Postives = 102/219 (46.58%), Query Frame = 0

Query: 517 SLLESEAKATLCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCVVEMLLSYA 576
           S L  + K  +   +++ I E I    E I   A   I   +V+LT GSS  V   L  A
Sbjct: 170 SSLTRKLKHDVIEGVNQLIQE-IAGCHEQIAEQAIEHIHQNEVILTLGSSRTVLEFLCAA 229

Query: 577 HDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVNRVFLGAAAVL 636
            +  + FRV V +  P+ +G  L + L+A+GL  T    +A+  ++  VN V +GA AV+
Sbjct: 230 KEKKRSFRVFVAEGAPRYQGHLLAKELVARGLQTTVITDSAVFAMISRVNMVIIGAHAVM 289

Query: 637 ANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLD-SICFNELGDPDAISKVSG 696
           ANG V   VG    A+ A    VP +    ++K       +  +  NEL  P  +     
Sbjct: 290 ANGGVIGPVGVNMAALAAQKHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGE 349

Query: 697 RTDINYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYG 735
            +D   LD       LQ++N  +D  P + +S+ +TD G
Sbjct: 350 FSDC--LDFGAGSGSLQVVNPTFDYVPPNLVSLFITDTG 385