Spg022527 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg022527
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionTranscription initiation factor IIE subunit alpha
Locationscaffold2: 3897024 .. 3902738 (+)
RNA-Seq ExpressionSpg022527
SyntenySpg022527
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCGGCTTTCAGATCTCTCTCGTCTCTGCAAAGTCTTGCTCGAATCGAACACCGAAAATAATTCAGCTCACATGTAATATCAACCTCAGCCCTGATTGTTTGATCGAGGAATTGAACGCTTGTTAATTTATCATCACCATGAGTATCGAACCTTTTAACAGGTACTTTCCTCTATGAACTTGTGAGCCCTCTCAAATGGTAAAATGGCAATTTGAATATCTTCTTCAGCCGTCGACAATGAGACTGTTTTCTGTTTTTGTTTTCTTCATGGGCTTGGTTTAATTTGTTTTCTCGAACGATGTTGATCCATTGGGCAGGTTGGTGAAGCTAGCTGCGAGAGCTTTCTACGATGATATTACGACTAAAGGGGATAATCAGCCCAAGACCGGTCGGAGTGACAATAGGGGAATCGCGGTTGTCGTTCTTGATGCTCTCACGAGGTACTGCTCTTTTTTGTTTTATTTACTTGGAAATTTTAGTGTCATGTACAAGGAAGGATTCCGTCGCTCATTTTGTTGTTGCTAAGCAACTGAGAGGAGAAGGGGTGGCTAGTGTTATTAGTGGAATGAATTCTATACAGGAACTGTGCTTAAATATGATTGGATTATTGGAAATTCGAATGAAACCTTCTTTCTTGCTTGTGAACGTTTTGCACTTTTAGCCAGATTCTTGGTAGTTATATTTCAAGAGTAAGCTGGGCACCGTATTGTGCACCCAATTCTGCTAGTGCTGGAGTGAAACACTAAATCAATGGTTGTTATATATGTTTCTTGAAACTTATATTTTATACGTTATTTCTCTTGTATGGTCTGAGCAAATCGCTGATTGAAAATGTCATTTTCAAATCTCTTAATTTATTATCATAAAGGATTTGGGATATGATCCTGCCAAAGGTTTGAAATTTTATGATGATGAAACAATGCGGGTATTGTATCTCCTTTTGTGAATCTGGTGAGCTGTTTTACTGCCACATTTCTGCTTTGCTTATCACATTTTTTTGTTGTATCCTGTCCTAGACGACAATGGGTTCGGGAAGAAGATTTGGCAAAGAACTTAAAACTACATTCGAAGCAATTACGTCGAACCCTAAGGTTTTTAGAAGAGGAAAAATTGATTACTCGAGATCATAGAAGGGAGGTCAGTGCTGACTCTATCTATGCATCTTGTTCTTGTGCCTATTAGGTTAGTAAACTTGGAATATCTTAGTATGCAATTTTCTGCATTTTAACTTGATTGTCTATAGCATCTAAGGTGTTTATGTCAAATGGTGTTTGTGCATTATTTCGGGAAATTTTGTTAAAAAGAAATGGAGAGGTGTTTCAAGGAGCTGAAAGCAGTTGGAGAGCCTTGGGAAGTTACTGTTTTTGTTGCCTCTTGATGCATTTCTATTAAATTTGTTTGTAATTACTCTCTCTCTCCTTTTATTGCCATTAGGAGAGTCTTTTTTCTAATTTCTGGTGGAATGTCCATTTTTTTAGTTCAAGAAAGAGGAAGAAAAAGGAAGTTATTGGCCAGAGGGAAGACCAAATGGGAGCTAAAGACAGACAGTAATGGGATTTAGAAAAAAAAAATTTCTTGTTGTCTTGGAGAAAATATTTAAAGTGCATAAATAAAATGCTCTACGAGCTTTTTTGTGATATAGACCTTTGATTGCATGAATGTCACTAGCACTTTGGCAAACAAATAAATATAGTTCATGTCGGATCCATCGATAGACGACTCGACTGCAGTTACTAGTGCCTACATTGCTATACCTTCTTTCTTGGACTAGACATGCGGACAATCCACCTTTTTCTCTTGTATGCGCTATGCCATTTTAGATTTTCACTAGAGGGGAGATTGAAAAATAAGAATAAAGATTGGAGTTAAGTCGAATGATGGTCACTCTCATCAACTCTGCACTTTTTTCTCTCTCATCTTACCTGTTATCTTCTTGTATTTCTTCATCCCAGGATGATTAACATATGTGTTATGTAAACTTGTCCGTGTTTTTCATACAGCAAATTCTATTTGAATTCTGTTACACAGCAAATTCTATTTGAATTCTGTTACACAGCAAATTCTATTTGAATTCTGTTAAACATATTGCTTAATAATTCGTACTTTTTAATATGGAGCAGCTGTGTCAATGAGTTATCCTTTCCTAACATGATAACATTATTTATAGGAATGCTCTTCATTTTCTTTGTTCTTTGCTTTTCCAGACAGCCAAGGGAGCAAAGATATTTAGTGCTGCAGTTGCTGCCACAGCTGATGGTCAACACCCAGTGAAGGAGGGGGATGAAAAGATTAAGATGCACACGCACTCTTATTGCTCTCTAGACTATGCACAGGTTTCTTTTCTATATGTTATTTTTATGGTTGCTTCATAACTAATTAATAATTGTATCTAAACCCAATGGAAACAGACTTAATTTTGATCAGTTACCTTATTAAATCTAAACTTGGTTACAGATCTATGATGTGGTTAGATACAGAATGCACCGCATGAAGAAAAAACTGAAAGATGAATTAGAGGACAAGAACACAGTCCAGGAGTATATATGCCCCAACTGTTCGAGAAGGTTTTGATGATATGTTCTTTCTCTCTTAAAAATGTTCTTTCTAATATTTTAAACTGGAACCACAGCACACTTGTTATTGACTTCTTTACCTGGAAGCAAGCTTGAAAAATTTATGACATTGATTAGGTACACTGCTTTGGATGCTCTGCGATTAATCTCTCTTGAGGACGAATACTTCCACTGTGAAAATTGCAATGGAGAGCTAGTTGCAGAGAGTGATAAACTGGCTGCTGCACAAGGAGTGGATGGAGATGATAATGCCAGGAAGCGTCGACATGAAAAATTAAAGGACATGCTTCAGAAGATGGAGGTTTGATATCTTGAAATAGAATTTTATTTTTCAATGTGTCGTGCATTTTACTTTAATGGATTTTACATTTTGTACAATGACCGAAAAAAAGTGTTTTTCTGAATCATGTTCATTTTCAATTTTTTTCCCTTTAAATTGTTTCTTTTCTTTTTTTTAATCGTATTGATCTTGAAAGAATTATATCCTGGATACGAGAACACAAGGTATTATTCTATGCTGTAATGAGCTTATTATTTTCTTTAAATAATGAACACCTGTTCTGTAGAAAATTCATTATTTCTGTATACTTCAGAATTTATAGGGATCATTGATTGCTGTATTTTTTTACCTTTGTTAGTGTAATATAACACTGACCTTCAACTTCAAACCATGTTTCTGAAAAAATTTATGAGGGATTTGTGTTTTTACCCTTGAGTTTATGTAAAAGGATTTTTCTGTTGCTAAATGTTGATGAATAAATCAAATAATTGCAGGTCCAGCTTAAACCTTTAATGGAACAACTCAGCAGAGTCAAAGATTTGCCAGTTCCAGAATTTGGAACTCTTTTAGCATGGGAAGCTCGTGCAAGTGCAGCTGCACGTGCAGCAAATGGTGATCTCAATGGCAATGATCCCTCAAAATCATCACAAGGTTTAGGATATGGGGGAACACCAATGCCTTTTGTTGGAGAGACCAAGGTATCCCATTTTTTTTGCCTCATGCTTGTTATATTGTACCATTCTAACGGTCGAGTTTTTTAATGTATCTTTTAGGTTGAAGTTGCCTTTTCTGGGGCTGAGGGCAAGGGAGCAGATGTTAAATCTGAATCCGAGAACACAGGTCTGAAAGTTTTACCTCCTTGGATGATCAAGAAAGGAATGAACCTCACGAAGGAACAACGTGGGGAGGTCAAAGAGGAGTCAAAGATGGATGCTGGTTCAGCTTCTGCTCAGTTTTCTGATGATAAAAAGTCATCGGCCAATGAGGATGATAAAACGAATATACAGGCTTGTTCAATTATATTAGCTCTTTTCATTTCTTACTCATAACCAAGTGCTTTTTTTTATCCCAAAATCTAAAATTGAAAGAGAATACATGATCTCATGTACTGGTTGACTGTATTGTAGGATGAGTATGTTAAAGCTTATTATGCTGCTATACTGAAGAAGCAACAAGAGCTTGAAGAAGCTGCTAAGGGTCAACAGGAATTTTCCAGTACAGAGGTTACTGAAAATGTGTCTAGCACAAATTCCAGTCGCCAGGTGGGAATGAAGGCAAAACGTGAAGAGGATGAAGAAGATGATGTTGAGTGGGAGGAGGCTCCAATAGGGGGTGAGACATTACTTTTCTTTTCCATGTTTGACATTTTAATTTTTTCTGGTGTTTATATCTTCTTATTATTCTGCTGTGCAGTATATAAGTACCTTGACAGTACCATATATTAGTAAATGACATAAACAGCGTCACATACTTTATCTGTCTCAATATTGAGCATGGATTTATATGAGAAAGAATATGTTTAGTCTGAGAAATAGCTTCTTGTTAATCATCATTCTTGTGTGTATAAATTTTATCATCCTTGCACAATTTTATTGGAGGTCCCTGAAGGGATTTCGTGAATTTTATGTTTCACGCGACTTAAATGTTTTACTCATTATTTGTGTTTGGAACTGTGGACTGATTATCCAATGAAAAAAAATAATAAATAAATAATTAAGATAAAAATAAAAAACTAGATTTTATTGTTTTAAAACTTAACTTACAAATTGCAACCAAAATTGTATTGTTTTAAGCCCTTTCATACCATACCAATTTTTGAAATGAATAGTGGTTTCTAGTTTGCATATCAAAGTTACTGGTGTGTCCTTGGAGTTTTCAGAAGACATTTAATGACGGAATGCTTTGGGTTCTTAATTATTAAATTCAGTCTTACATTAGATTTTATTATGTTTCCTAGACTAAATTTATAAAGATGTTGAAATTAACAGTTGAACTTTCCTTTAATTGGTTGTGGCATATTTTATGTGTAGAAGTAAGTTGGGCTAAGTTCTTCAAAAAAAGTAGGTTGGGTTAGGGAAGATCTTTTCTGGTTTGACTTTAATCTACTATCTATTACTGTATGTTGCTTGCCTCCAATCACCAAGTTTCTTACCTTTCATACCGTATTACAGGTAGTGCTAATGAAAATTACAAGGTTGGTGACTTGAATGTTGAAGCATCAGCTTCGGTAGAGGATGAAGAAGAAGATGATGTGGATTGGGAGGAAGGTTGAAGAAGCATAGAGAAATTTTATACCCCTAATCTGACACAAGGTCATATTGCCTTTCTTCCATCTGCAAGGCCTTAATGAATTGAGGTAGGAGCTCTGCAGTTAATCTTGAGCGGCCGTGTTACTTGTTATGTTGGAGCCGAATTTAAAGATCCCTAGCCTATAATTGCTTGATTTACTGCTGAAATTTCATCTTGGGTACGATGATGAATTGAATTGTTCTTTACCCGTTTGGTGTGCTGGAAGAAAAGATCAAGGAAAATATATATTAATTGATTTATCACTCACCAATTAAGTTGGAAGAAGAGAAGGAATGTCAATACATTTGTTACCAAATTACAGTGGGATAAACTCGTGTGCTAACTTACTATTGGAGCAATACGGTGTATTTTACAACAGCTTTTACTTCTTTAGCTGATAGAGAAGTTTAGAAATAAAGACAGCGATTGCCAAACATTTTCTCCCTACTCTCCCTGTAGAAAAAACAGAACATAGCAAGTTAATATCATTTTAAACATAATGCATAGGCTTTATTTGTATGCTTATATTGACATTCATTGATCACAGTGACAATCAACACCTACCTATTGTCAG

mRNA sequence

ATGAGTATCGAACCTTTTAACAGGTTGGTGAAGCTAGCTGCGAGAGCTTTCTACGATGATATTACGACTAAAGGGGATAATCAGCCCAAGACCGGTCGGAGTGACAATAGGGGAATCGCGGTTGTCGTTCTTGATGCTCTCACGAGACGACAATGGGTTCGGGAAGAAGATTTGGCAAAGAACTTAAAACTACATTCGAAGCAATTACGTCGAACCCTAAGGTTTTTAGAAGAGGAAAAATTGATTACTCGAGATCATAGAAGGGAGACAGCCAAGGGAGCAAAGATATTTAGTGCTGCAGTTGCTGCCACAGCTGATGGTCAACACCCAGTGAAGGAGGGGGATGAAAAGATTAAGATGCACACGCACTCTTATTGCTCTCTAGACTATGCACAGATCTATGATGTGGTTAGATACAGAATGCACCGCATGAAGAAAAAACTGAAAGATGAATTAGAGGACAAGAACACAGTCCAGGAGTATATATGCCCCAACTGTTCGAGAAGGTACACTGCTTTGGATGCTCTGCGATTAATCTCTCTTGAGGACGAATACTTCCACTGTGAAAATTGCAATGGAGAGCTAGTTGCAGAGAGTGATAAACTGGCTGCTGCACAAGGAGTGGATGGAGATGATAATGCCAGGAAGCGTCGACATGAAAAATTAAAGGACATGCTTCAGAAGATGGAGGTCCAGCTTAAACCTTTAATGGAACAACTCAGCAGAGTCAAAGATTTGCCAGTTCCAGAATTTGGAACTCTTTTAGCATGGGAAGCTCGTGCAAGTGCAGCTGCACGTGCAGCAAATGGTGATCTCAATGGCAATGATCCCTCAAAATCATCACAAGGTTTAGGATATGGGGGAACACCAATGCCTTTTGTTGGAGAGACCAAGGTTGAAGTTGCCTTTTCTGGGGCTGAGGGCAAGGGAGCAGATGTTAAATCTGAATCCGAGAACACAGGTCTGAAAGTTTTACCTCCTTGGATGATCAAGAAAGGAATGAACCTCACGAAGGAACAACGTGGGGAGGTCAAAGAGGAGTCAAAGATGGATGCTGGTTCAGCTTCTGCTCAGTTTTCTGATGATAAAAAGTCATCGGCCAATGAGGATGATAAAACGAATATACAGGCTTCTTATTATGCTGCTATACTGAAGAAGCAACAAGAGCTTGAAGAAGCTGCTAAGGGTCAACAGGAATTTTCCAGTACAGAGGTTACTGAAAATGTGTCTAGCACAAATTCCAGTCGCCAGGTGGGAATGAAGGCAAAACGTGAAGAGGATGAAGAAGATGATGTTGAGTGGGAGGAGGCTCCAATAGGGGGTAGTGCTAATGAAAATTACAAGGTTGGTGACTTGAATGTTGAAGCATCAGCTTCGGTAGAGGATGAAGAAGAAGATGATGTGGATTGGGAGGAAGGTTGA

Coding sequence (CDS)

ATGAGTATCGAACCTTTTAACAGGTTGGTGAAGCTAGCTGCGAGAGCTTTCTACGATGATATTACGACTAAAGGGGATAATCAGCCCAAGACCGGTCGGAGTGACAATAGGGGAATCGCGGTTGTCGTTCTTGATGCTCTCACGAGACGACAATGGGTTCGGGAAGAAGATTTGGCAAAGAACTTAAAACTACATTCGAAGCAATTACGTCGAACCCTAAGGTTTTTAGAAGAGGAAAAATTGATTACTCGAGATCATAGAAGGGAGACAGCCAAGGGAGCAAAGATATTTAGTGCTGCAGTTGCTGCCACAGCTGATGGTCAACACCCAGTGAAGGAGGGGGATGAAAAGATTAAGATGCACACGCACTCTTATTGCTCTCTAGACTATGCACAGATCTATGATGTGGTTAGATACAGAATGCACCGCATGAAGAAAAAACTGAAAGATGAATTAGAGGACAAGAACACAGTCCAGGAGTATATATGCCCCAACTGTTCGAGAAGGTACACTGCTTTGGATGCTCTGCGATTAATCTCTCTTGAGGACGAATACTTCCACTGTGAAAATTGCAATGGAGAGCTAGTTGCAGAGAGTGATAAACTGGCTGCTGCACAAGGAGTGGATGGAGATGATAATGCCAGGAAGCGTCGACATGAAAAATTAAAGGACATGCTTCAGAAGATGGAGGTCCAGCTTAAACCTTTAATGGAACAACTCAGCAGAGTCAAAGATTTGCCAGTTCCAGAATTTGGAACTCTTTTAGCATGGGAAGCTCGTGCAAGTGCAGCTGCACGTGCAGCAAATGGTGATCTCAATGGCAATGATCCCTCAAAATCATCACAAGGTTTAGGATATGGGGGAACACCAATGCCTTTTGTTGGAGAGACCAAGGTTGAAGTTGCCTTTTCTGGGGCTGAGGGCAAGGGAGCAGATGTTAAATCTGAATCCGAGAACACAGGTCTGAAAGTTTTACCTCCTTGGATGATCAAGAAAGGAATGAACCTCACGAAGGAACAACGTGGGGAGGTCAAAGAGGAGTCAAAGATGGATGCTGGTTCAGCTTCTGCTCAGTTTTCTGATGATAAAAAGTCATCGGCCAATGAGGATGATAAAACGAATATACAGGCTTCTTATTATGCTGCTATACTGAAGAAGCAACAAGAGCTTGAAGAAGCTGCTAAGGGTCAACAGGAATTTTCCAGTACAGAGGTTACTGAAAATGTGTCTAGCACAAATTCCAGTCGCCAGGTGGGAATGAAGGCAAAACGTGAAGAGGATGAAGAAGATGATGTTGAGTGGGAGGAGGCTCCAATAGGGGGTAGTGCTAATGAAAATTACAAGGTTGGTGACTTGAATGTTGAAGCATCAGCTTCGGTAGAGGATGAAGAAGAAGATGATGTGGATTGGGAGGAAGGTTGA

Protein sequence

MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQHPVKEGDEKIKMHTHSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLEDEYFHCENCNGELVAESDKLAAAQGVDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSRVKDLPVPEFGTLLAWEARASAAARAANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVEVAFSGAEGKGADVKSESENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQFSDDKKSSANEDDKTNIQASYYAAILKKQQELEEAAKGQQEFSSTEVTENVSSTNSSRQVGMKAKREEDEEDDVEWEEAPIGGSANENYKVGDLNVEASASVEDEEEDDVDWEEG
Homology
[Close]
BLAST of Spg022527 vs. NCBI nr
Match: XP_038880289.1 (general transcription factor IIE subunit 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 871.3 bits (2250), Expect = 3.8e-249
Identity = 455/477 (95.39%), Postives = 464/477 (97.27%), Query Frame = 0 

Query: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60
           MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK
Sbjct: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60

Query: 61  NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQHPVKEGDEKIKM 120
           NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQHPVKEGDEKIKM
Sbjct: 61  NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQHPVKEGDEKIKM 120

Query: 121 HTHSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180
           HTHSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS
Sbjct: 121 HTHSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180

Query: 181 LEDEYFHCENCNGELVAESDKLAAAQGVDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQL 240
            EDEYFHCENCNGELVAESDKLAAAQG DGDDNARKRRHEKLKDMLQKMEVQLKPLMEQL
Sbjct: 181 FEDEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQL 240

Query: 241 SRVKDLPVPEFGTLLAWEARASAAARAANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVE 300
           SRVKDLPVPEFGTLLAWEARASAAAR ANGDLNGNDPSKS+QGLGYGGTPMPFVGETKVE
Sbjct: 241 SRVKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSAQGLGYGGTPMPFVGETKVE 300

Query: 301 VAFSGAEGKGADVKSESENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQFS 360
           VAFSGAEGKG DVKSESENTGLKVLPPWMIKKGMNLTKEQRGEVK+ESKMDAGSASAQFS
Sbjct: 301 VAFSGAEGKGVDVKSESENTGLKVLPPWMIKKGMNLTKEQRGEVKDESKMDAGSASAQFS 360

Query: 361 DDKKSSANEDDKTNIQ----ASYYAAILKKQQELEEAAKGQQEFSSTEVTENVSSTNSSR 420
           DDKKSSAN+DDKTNIQ     +YYAAILKKQQELEEAAKGQ+E  STEVTENV++ NSSR
Sbjct: 361 DDKKSSANDDDKTNIQDEYVKAYYAAILKKQQELEEAAKGQEELPSTEVTENVANRNSSR 420

Query: 421 QVGMKAKREEDEEDDVEWEEAPIGGSANENYKVGDLNVEASASVEDEEEDDVDWEEG 474
           QVGMKAKREEDEEDD+EWEEAPIGG+ANENYKV DLNVEASASVEDEEEDDVDWEEG
Sbjct: 421 QVGMKAKREEDEEDDIEWEEAPIGGNANENYKV-DLNVEASASVEDEEEDDVDWEEG 476

[Close]
BLAST of Spg022527 vs. NCBI nr
Match: XP_038880290.1 (general transcription factor IIE subunit 1 isoform X2 [Benincasa hispida])

HSP 1 Score: 864.4 bits (2232), Expect = 4.6e-247
Identity = 454/477 (95.18%), Postives = 463/477 (97.06%), Query Frame = 0 

Query: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60
           MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK
Sbjct: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60

Query: 61  NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQHPVKEGDEKIKM 120
           NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQHPVKEGDEKIKM
Sbjct: 61  NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQHPVKEGDEKIKM 120

Query: 121 HTHSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180
           HTHSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS
Sbjct: 121 HTHSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180

Query: 181 LEDEYFHCENCNGELVAESDKLAAAQGVDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQL 240
            EDEYFHCENCNGELVAESDKLAAAQG DGDDNARKRRHEKLKDMLQKMEVQLKPLMEQL
Sbjct: 181 FEDEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQL 240

Query: 241 SRVKDLPVPEFGTLLAWEARASAAARAANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVE 300
           SRVKDLPVPEFGTLLAWEARASAAAR ANGDLNGNDPSKS+QGLGYGGTPMPFVGETKVE
Sbjct: 241 SRVKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSAQGLGYGGTPMPFVGETKVE 300

Query: 301 VAFSGAEGKGADVKSESENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQFS 360
           VAFSGAEGKG DVKSESENTGLKVLPPWMIKKGMNLTKEQRGEVK+ESKMDAGSASAQFS
Sbjct: 301 VAFSGAEGKGVDVKSESENTGLKVLPPWMIKKGMNLTKEQRGEVKDESKMDAGSASAQFS 360

Query: 361 DDKKSSANEDDKTNIQ----ASYYAAILKKQQELEEAAKGQQEFSSTEVTENVSSTNSSR 420
           DDKKSSAN+DDKTNIQ     +YYAAILKKQQELEEAAKGQ+E  STEVTENV++ NSSR
Sbjct: 361 DDKKSSANDDDKTNIQDEYVKAYYAAILKKQQELEEAAKGQEELPSTEVTENVANRNSSR 420

Query: 421 QVGMKAKREEDEEDDVEWEEAPIGGSANENYKVGDLNVEASASVEDEEEDDVDWEEG 474
           QVGMKAKREEDEEDD+EWEEAPI G+ANENYKV DLNVEASASVEDEEEDDVDWEEG
Sbjct: 421 QVGMKAKREEDEEDDIEWEEAPI-GNANENYKV-DLNVEASASVEDEEEDDVDWEEG 475

[Close]
BLAST of Spg022527 vs. NCBI nr
Match: XP_023515738.1 (general transcription factor IIE subunit 1-like [Cucurbita pepo subsp. pepo] >XP_023515739.1 general transcription factor IIE subunit 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 860.5 bits (2222), Expect = 6.7e-246
Identity = 449/478 (93.93%), Postives = 462/478 (96.65%), Query Frame = 0 

Query: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60
           MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK
Sbjct: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60

Query: 61  NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQHPVKEGDEKIKM 120
           NLKLHSKQLRRTLRFLEEEKL+TRDHRRETAKGAKIFSAA+AATADGQHP KEGDEKIKM
Sbjct: 61  NLKLHSKQLRRTLRFLEEEKLVTRDHRRETAKGAKIFSAAIAATADGQHPGKEGDEKIKM 120

Query: 121 HTHSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180
           HTHSYCSLDYAQ+YDVVRYR+HRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS
Sbjct: 121 HTHSYCSLDYAQVYDVVRYRLHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180

Query: 181 LEDEYFHCENCNGELVAESDKLAAAQGVDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQL 240
           LEDEYFHCENCNGELVAESDKLAAAQG DGDDNARKRRHEKLKDMLQKMEVQLKPLMEQL
Sbjct: 181 LEDEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQL 240

Query: 241 SRVKDLPVPEFGTLLAWEARASAAARAANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVE 300
           SRVKDLPVPEFGTLLAWEARASAAAR ANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVE
Sbjct: 241 SRVKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVE 300

Query: 301 VAFSGAEGKGADVKSESENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQFS 360
           VAFSGAEGKG +VKSESENT LKVLPPWMIK+GMNLTKEQRGEVK E+KMDAGSAS QFS
Sbjct: 301 VAFSGAEGKGVNVKSESENTTLKVLPPWMIKEGMNLTKEQRGEVKGEAKMDAGSASTQFS 360

Query: 361 DDKKSSANEDDKTNIQ----ASYYAAILKKQQELEEAAKGQQEFSSTEVTENVSSTNSSR 420
           DDKKSSAN+DDKTNIQ     +YYAAILKKQQELEEAAKGQ+E SSTEVTEN+S TNSSR
Sbjct: 361 DDKKSSANDDDKTNIQDEYVKAYYAAILKKQQELEEAAKGQKELSSTEVTENLSYTNSSR 420

Query: 421 QVGMKAKREE-DEEDDVEWEEAPIGGSANENYKVGDLNVEASASVEDEEEDDVDWEEG 474
           QVGMKAKREE DEEDD+EWEEAPI G+ANENYKVGDLNVEASASVEDEEEDDVDWEEG
Sbjct: 421 QVGMKAKREEDDEEDDIEWEEAPIDGNANENYKVGDLNVEASASVEDEEEDDVDWEEG 478

[Close]
BLAST of Spg022527 vs. NCBI nr
Match: XP_022922068.1 (general transcription factor IIE subunit 1-like [Cucurbita moschata] >KAG6589563.1 General transcription factor IIE subunit 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023252.1 General transcription factor IIE subunit 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 860.1 bits (2221), Expect = 8.7e-246
Identity = 449/478 (93.93%), Postives = 462/478 (96.65%), Query Frame = 0 

Query: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60
           MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK
Sbjct: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60

Query: 61  NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQHPVKEGDEKIKM 120
           NLKLHSKQLRRTLRFLEEEKL+TRDHRRETAKGAKIFSAA+AATADGQHP KEGDEKIKM
Sbjct: 61  NLKLHSKQLRRTLRFLEEEKLVTRDHRRETAKGAKIFSAAIAATADGQHPGKEGDEKIKM 120

Query: 121 HTHSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180
           HTHSYCSLDYAQ+YDVVRYR+HRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS
Sbjct: 121 HTHSYCSLDYAQVYDVVRYRLHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180

Query: 181 LEDEYFHCENCNGELVAESDKLAAAQGVDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQL 240
           LEDEYFHCENCNGELVAESDKLAAAQG DGDDNARKRRHEKLKDMLQKMEVQLKPLMEQL
Sbjct: 181 LEDEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQL 240

Query: 241 SRVKDLPVPEFGTLLAWEARASAAARAANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVE 300
           SRVKDLPVPEFGTLLAWEARASAAAR ANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVE
Sbjct: 241 SRVKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVE 300

Query: 301 VAFSGAEGKGADVKSESENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQFS 360
           VAFSGAEGKG +VKSESENT LKVLPPWMIK+GMNLTKEQRGEVK +SKMDAGSASAQFS
Sbjct: 301 VAFSGAEGKGVNVKSESENTALKVLPPWMIKEGMNLTKEQRGEVKGDSKMDAGSASAQFS 360

Query: 361 DDKKSSANEDDKTNIQ----ASYYAAILKKQQELEEAAKGQQEFSSTEVTENVSSTNSSR 420
           DDKKSSAN+DDKTNIQ     +YYAAILKKQQELEEAAK Q+E SSTEVTEN+S TNSSR
Sbjct: 361 DDKKSSANDDDKTNIQDEYVKAYYAAILKKQQELEEAAKAQKELSSTEVTENLSYTNSSR 420

Query: 421 QVGMKAKREED-EEDDVEWEEAPIGGSANENYKVGDLNVEASASVEDEEEDDVDWEEG 474
           QVGMKAKREED EEDD+EWEEAPI G+ANENYKVGDLNVEASASVEDEEEDDVDWEEG
Sbjct: 421 QVGMKAKREEDEEEDDIEWEEAPIEGNANENYKVGDLNVEASASVEDEEEDDVDWEEG 478

[Close]
BLAST of Spg022527 vs. NCBI nr
Match: XP_022987307.1 (general transcription factor IIE subunit 1-like [Cucurbita maxima])

HSP 1 Score: 857.1 bits (2213), Expect = 7.4e-245
Identity = 447/478 (93.51%), Postives = 461/478 (96.44%), Query Frame = 0 

Query: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60
           MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK
Sbjct: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60

Query: 61  NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQHPVKEGDEKIKM 120
           NLKLHSKQLRRTLRFLEEEKL+TRDHRRETAKGAKIFSAAVAATADGQHP KEGDEKIKM
Sbjct: 61  NLKLHSKQLRRTLRFLEEEKLVTRDHRRETAKGAKIFSAAVAATADGQHPGKEGDEKIKM 120

Query: 121 HTHSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180
           HTHSYCSLDYAQ+YDVVRYR+HRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS
Sbjct: 121 HTHSYCSLDYAQVYDVVRYRLHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180

Query: 181 LEDEYFHCENCNGELVAESDKLAAAQGVDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQL 240
           LEDEYFHCENCNGELVAESDKLAAAQG DGDDNARKRRHEKLKDMLQKMEVQLKPLMEQL
Sbjct: 181 LEDEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQL 240

Query: 241 SRVKDLPVPEFGTLLAWEARASAAARAANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVE 300
           SRVKDLPVPEFGTLLAWEARASAAAR ANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVE
Sbjct: 241 SRVKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVE 300

Query: 301 VAFSGAEGKGADVKSESENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQFS 360
           VAFSGAE KG +VKSESENT LKVLPPWMIK+GMNLTKEQRGEVK ESKMDAGSASAQFS
Sbjct: 301 VAFSGAESKGVNVKSESENTALKVLPPWMIKEGMNLTKEQRGEVKGESKMDAGSASAQFS 360

Query: 361 DDKKSSANEDDKTNIQ----ASYYAAILKKQQELEEAAKGQQEFSSTEVTENVSSTNSSR 420
           DDKKSSAN+DDKTNIQ     +YYAAILKKQQELEEAAKGQ+E S TE+T+N+S TN+SR
Sbjct: 361 DDKKSSANDDDKTNIQDEYVKAYYAAILKKQQELEEAAKGQKELSRTEITDNLSYTNASR 420

Query: 421 QVGMKAKREED-EEDDVEWEEAPIGGSANENYKVGDLNVEASASVEDEEEDDVDWEEG 474
           QVGMKAKREED EEDD+EWEEAPI G+ANENYKVGDLNVEASASVEDEEEDDVDWEEG
Sbjct: 421 QVGMKAKREEDEEEDDIEWEEAPIEGNANENYKVGDLNVEASASVEDEEEDDVDWEEG 478

[Close]
BLAST of Spg022527 vs. ExPASy Swiss-Prot
Match: Q557M8 (General transcription factor IIE subunit 1 OS=Dictyostelium discoideum OX=44689 GN=gtf2e1-1 PE=3 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 2.7e-16
Identity = 110/468 (23.50%), Postives = 194/468 (41.45%), Query Frame = 0 

Query: 9   LVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNLKLHSKQ 68
           LVK+  RAFY D                   AV++   L  ++ +++EDLA  L++  K 
Sbjct: 14  LVKMVIRAFYPD-----------------EYAVIIDGLLREKKRIKDEDLALRLRIQQKY 73

Query: 69  LRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQHPVKEGDEKIKMHTHSYCSL 128
           +R+ L  L+ + ++     +  AKG                P + G       TH    +
Sbjct: 74  VRKILMDLKGDSMVKSSDVKVEAKG----------------PNERGS------THLLWYI 133

Query: 129 DYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLEDEYFHC 188
           DY  I D+V+Y+++  +KK++     K  VQ Y C  C + YTALD  +L++++     C
Sbjct: 134 DYKHIIDIVKYKLYMFRKKMESVKVQKIDVQTYKCQTCHKVYTALDIPKLLNMDTGALAC 193

Query: 189 ENCNGELVAESDKLAAAQGVDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSRVKDLPV 248
           E C+GEL  E         ++ +   +  +H+   D+      QL+ ++EQL + +   +
Sbjct: 194 EICDGELEEE---------LNNESLTQTAKHQ--SDLFS----QLRKIIEQLKKTEGHNI 253

Query: 249 PEFGTLLAWEARASAAARAANGDLN-GNDPSKSSQGLGYGGTPMPFVGETKVEVAF---- 308
           P F   LA  +     +   N + + G  P  S+  +  G      +  T   + F    
Sbjct: 254 PLFARDLADLSADQGPSYTINTNSSLGMGPKPSAFPVAQGAATSHHIDPTNENIEFHVDI 313

Query: 309 ---SGAEGKGADVKSESENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQFS 368
               G E   A VK E++ TGL  LPPW++    N  K +   VK  S ++    +   +
Sbjct: 314 LDTDGIEINKAVVKKENKKTGLASLPPWLLPS--NSFKNR--NVKSNSILNNNQQTQTST 373

Query: 369 DDKKSSANEDDKTNIQASYYAAILKKQQELEEAAKGQQEFSSTEVTENVSSTNSSRQVGM 428
           +++ ++  E  K  I   +Y   +K   +  E+A       S +   N S++ S      
Sbjct: 374 NEQPTAVKEQIK--IDQDFYINYIKTHYQEWESAP-----DSGDADGNGSNSGSGGST-- 403

Query: 429 KAKREEDEEDDVEWEEAPIGGSANENYKVGDLNVEASASVEDEEEDDV 469
                      +E  +   G   N+  K+ D    +S S  D++  D+
Sbjct: 434 -----------IEGNDGGNGEHQNKKMKLDDSQTVSSMSQSDDDGKDI 403

[Close]
BLAST of Spg022527 vs. ExPASy Swiss-Prot
Match: P36100 (Transcription initiation factor IIE subunit alpha OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TFA1 PE=1 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 7.9e-08
Identity = 107/477 (22.43%), Postives = 192/477 (40.25%), Query Frame = 0 

Query: 38  GIAVVVLDALTRRQWVREEDLAKNLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIF 97
           G  V+VLDA+     + E+DL + L ++  +L   +  L  ++LI+   +RE        
Sbjct: 23  GSFVLVLDAILFHSVLAEDDLKQLLSINKTELGPLIARLRSDRLISIHKQRE-------- 82

Query: 98  SAAVAATADGQHPVKEGDEKIKMHTHSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNT 157
                       P  +  E++      Y  + Y    D +++++H++ ++LKD+L+  + 
Sbjct: 83  ----------YPPNSKSVERV------YYYVKYPHAIDAIKWKVHQVVQRLKDDLDKNSE 142

Query: 158 VQEYICPNCSRRYTALDALRLISLEDEYFHCENCNGELVAESDKLAAAQGVDGDDNARKR 217
              Y+CP C  +YT L+A++L++ +   F C  C+  LV              +D++ K+
Sbjct: 143 PNGYMCPICLTKYTQLEAVQLLNFDRTEFLCSLCDEPLV--------------EDDSGKK 202

Query: 218 RHEKLKDMLQKMEVQLKPLMEQLSRVKDLPVPE--FGTLLA------------------- 277
             EK +D L ++  Q++P+++ L ++ D  + E  F   LA                   
Sbjct: 203 NKEK-QDKLNRLMDQIQPIIDSLKKIDDSRIEENTFEIALARLIPPQNQSHAAYTYNPKK 262

Query: 278 ----WEARASAAARAANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVEVAFSGAEGKGAD 337
               +    SA      G   GND S+ + G     T    +     EVA    + + A+
Sbjct: 263 GSTMFRPGDSAPLPNLMGTALGNDSSRRA-GANSQATLHINITTASDEVAQRELQERQAE 322

Query: 338 VKSESENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMD-AGSASAQFS----------- 397
            K +        +P W   K   + K   G +  E + D   +ASA  S           
Sbjct: 323 EKRKQ-----NAVPEW--HKQSTIGKTALGRLDNEEEFDPVVTASAMDSINPDNEPAQET 382

Query: 398 --------DDKKSSANEDDKTNIQASYYAAILKKQQELEEAAKGQQEFSSTEVTENVSST 457
                    +++    E++KT     YYAA+ KKQ +L +  + ++E    E  E     
Sbjct: 383 SYQNNRTLTEQEMEERENEKT--LNDYYAALAKKQAKLNKEEEEEEEEEEDEEEEEEEEM 442

Query: 458 NSSRQVGMKAKREEDEEDDVEWEEAPIGGSANENYKVGDLNVEASASVEDEEEDDVD 470
                   +  RE   ED+ E      G +  E+    D+  E   S+ D+ ED V+
Sbjct: 443 EDVMDDNDETARENALEDEFEDVTDTAGTAKTESNTSNDVKQE---SINDKTEDAVN 447

[Close]
BLAST of Spg022527 vs. ExPASy Swiss-Prot
Match: Q5R8H5 (General transcription factor IIE subunit 1 OS=Pongo abelii OX=9601 GN=GTF2E1 PE=2 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 1.0e-07
Identity = 95/413 (23.00%), Postives = 164/413 (39.71%), Query Frame = 0 

Query: 42  VVLDALTRRQWVREEDLAKNLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAV 101
           + LD L R   V+EED+ + LK   KQLR  L  L+ +K I    R ETA          
Sbjct: 32  LALDILIRNPCVKEEDMLELLKFDRKQLRSVLNNLKGDKFIKCRMRVETA---------- 91

Query: 102 AATADGQHPVKEGDEKIKMHTHSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEY 161
              ADG           K   H+Y  ++Y  + +VV+Y++  M+++++ +  D      +
Sbjct: 92  ---ADG-----------KTTRHNYYFINYRTLVNVVKYKLDHMRRRIETDERDSTNRASF 151

Query: 162 ICPNCSRRYTALDALRLISLEDEYFHCENCNGELVAESDKLAAAQGVDGDDNARKRRHEK 221
            CP CS  +T L+A +L       F C  C+ E             V+ D++A  ++   
Sbjct: 152 KCPVCSSTFTDLEANQLFDPMTGTFRCTFCHTE-------------VEEDESAMPKK--D 211

Query: 222 LKDMLQKMEVQLKPLMEQLSRVKDLPV------PEFGTLLAW-EARASAAARAANGDLNG 281
            + +L +   Q++P+   L   +D+ +      PE   + A  +++  AA  A    L G
Sbjct: 212 ARTLLARFNEQIEPIYALLRETEDVNLAYEILEPEPTEIPALKQSKDHAATTAGAASLAG 271

Query: 282 NDPSKSSQGLGYGGTPMPFVGETKVEVAFSGAEGKGADVKSESENTGLKVLPPWMIKKGM 341
               ++        T  P   +   +      +      ++  E    K  P W+ +  +
Sbjct: 272 GHHREA------WATKGPSYEDLYTQNVVINMDDHEDLHRASLEGKSAKERPIWLRESTV 331

Query: 342 NLTKEQRGEVKEESKMDAGSASAQFSDDKKSSANEDDKTNIQASYYAAILKKQQELEEAA 401
                 +G    E   + G     F + ++  A  DD   +      A+L  +++   A 
Sbjct: 332 ------QGAYSSEDMKEGGIDMDAFQEHEEGRAGPDDNEEVM----RALLIHEKKTSSAM 389

Query: 402 KGQQEFSSTEVTENVSSTNSS----------RQVGMKA-KREEDEEDDVEWEE 437
            G    ++   T N S + S           R   +   KREEDEE+D E+EE
Sbjct: 392 AGSVGAAAPVTTANGSDSESETSESDDDSPPRPAAVAVHKREEDEEEDDEFEE 389

[Close]
BLAST of Spg022527 vs. ExPASy Swiss-Prot
Match: P29083 (General transcription factor IIE subunit 1 OS=Homo sapiens OX=9606 GN=GTF2E1 PE=1 SV=2)

HSP 1 Score: 58.2 bits (139), Expect = 3.0e-07
Identity = 91/409 (22.25%), Postives = 163/409 (39.85%), Query Frame = 0 

Query: 42  VVLDALTRRQWVREEDLAKNLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAV 101
           + LD L R   V+EED+ + LK   KQLR  L  L+ +K I    R ETA          
Sbjct: 32  LALDILIRNSCVKEEDMLELLKFDRKQLRSVLNNLKGDKFIKCRMRVETA---------- 91

Query: 102 AATADGQHPVKEGDEKIKMHTHSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEY 161
              ADG           K   H+Y  ++Y  + +VV+Y++  M+++++ +  D      +
Sbjct: 92  ---ADG-----------KTTRHNYYFINYRTLVNVVKYKLDHMRRRIETDERDSTNRASF 151

Query: 162 ICPNCSRRYTALDALRLISLEDEYFHCENCNGELVAESDKLAAAQGVDGDDNARKRRHEK 221
            CP CS  +T L+A +L       F C  C+ E             V+ D++A  ++   
Sbjct: 152 KCPVCSSTFTDLEANQLFDPMTGTFRCTFCHTE-------------VEEDESAMPKK--D 211

Query: 222 LKDMLQKMEVQLKPLMEQLSRVKDLPV------PEFGTLLAW-EARASAAARAANGDLNG 281
            + +L +   Q++P+   L   +D+ +      PE   + A  +++  AA  A    L G
Sbjct: 212 ARTLLARFNEQIEPIYALLRETEDVNLAYEILEPEPTEIPALKQSKDHAATTAGAASLAG 271

Query: 282 NDPSKSSQGLGYGGTPMPFVGETKVEVAFSGAEGKGADVKSESENTGLKVLPPWMIKKGM 341
               ++        T  P   +   +      + +    ++  E    K  P W+ +  +
Sbjct: 272 GHHREA------WATKGPSYEDLYTQNVVINMDDQEDLHRASLEGKSAKERPIWLRESTV 331

Query: 342 NLTKEQRGEVKEESKMDAGSASAQFSDDKKSSANEDDKTNIQASYYAAILKKQQELEEAA 401
                 +G    E   + G     F + ++  A  DD   +  +      K    +  + 
Sbjct: 332 ------QGAYGSEDMKEGGIDMDAFQEREEGHAGPDDNEEVMRALLIHEKKTSSAMAGSV 389

Query: 402 KGQQEFSSTEVTENVSSTNSS------RQVGMKA-KREEDEEDDVEWEE 437
                 ++   +++ S T+ S      R   +   KREEDEE+D E+EE
Sbjct: 392 GAAAPVTAANGSDSESETSESDDDSPPRPAAVAVHKREEDEEEDDEFEE 389

[Close]
BLAST of Spg022527 vs. ExPASy Swiss-Prot
Match: A6QLI8 (General transcription factor IIE subunit 1 OS=Bos taurus OX=9913 GN=GTF2E1 PE=2 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 2.0e-06
Identity = 96/417 (23.02%), Postives = 166/417 (39.81%), Query Frame = 0 

Query: 42  VVLDALTRRQWVREEDLAKNLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAV 101
           + LD L R   V+EED+ + LK   KQLR  L  L+ +K I    R ETA          
Sbjct: 32  LALDILIRNPCVKEEDMLELLKFDRKQLRSVLNNLKGDKFIKCRMRVETA---------- 91

Query: 102 AATADGQHPVKEGDEKIKMHTHSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEY 161
              ADG           K   H+Y  ++Y  + +VV+Y++  M+++++ +  D      +
Sbjct: 92  ---ADG-----------KTTRHNYYFINYRTLVNVVKYKLDHMRRRIETDERDSTNRASF 151

Query: 162 ICPNCSRRYTALDALRLISLEDEYFHCENCNGELVAESDKLAAAQGVDGDDNARKRRHEK 221
            CP CS  +T L+A +L       F C  C  E             V+ D++A  ++   
Sbjct: 152 KCPVCSSTFTDLEANQLFDPMTGTFRCTFCQTE-------------VEEDESAMPKK--D 211

Query: 222 LKDMLQKMEVQLKPLMEQLSRVKDL--------PVP-EFGTLLAWEARASAAARAANGDL 281
            + +L +   Q++P+   L   +D+        P P E   L   + RA+ AA AA    
Sbjct: 212 ARTLLARFNEQIEPIYALLRETEDVNLAYEILEPEPTEIPALKQSKDRAATAAGAAGLAG 271

Query: 282 NGNDPSKSSQGLGYGGTPMPFV---GETKVEVAFSGAEGKGADVKSESENTGLKVLPPWM 341
             +  + +++G  Y       V    + + ++  +  EGK A           K  P W+
Sbjct: 272 GHHREAWTTKGPSYEDLYTQNVVINMDDQEDLHRASLEGKSA-----------KERPIWL 331

Query: 342 IKKGMNLTKEQRGEVKEESKMDAGSASAQFSDDKKSSANEDDKTNIQASYYAAILKKQQE 401
            +  +      +G    E   + G     F + ++  A  DD   +      A+L  +++
Sbjct: 332 RESTV------QGAYNSEEMKEGGIDIDSFQEHEEGHAGPDDNEEVM----RALLIHEKK 388

Query: 402 LEEAAKGQQEFSSTEVTENVSSTNSS----------RQVGMKAKREEDEEDDVEWEE 437
              A  G    ++     N S + S           R   + A   E++E+D E+EE
Sbjct: 392 TPSAPAGSVGAAAPVTAANGSDSESETSESDDDSPPRVATVAAHHGEEDEEDDEFEE 388

[Close]
BLAST of Spg022527 vs. ExPASy TrEMBL
Match: A0A6J1E5J2 (Transcription initiation factor IIE subunit alpha OS=Cucurbita moschata OX=3662 GN=LOC111430131 PE=3 SV=1)

HSP 1 Score: 860.1 bits (2221), Expect = 4.2e-246
Identity = 449/478 (93.93%), Postives = 462/478 (96.65%), Query Frame = 0 

Query: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60
           MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK
Sbjct: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60

Query: 61  NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQHPVKEGDEKIKM 120
           NLKLHSKQLRRTLRFLEEEKL+TRDHRRETAKGAKIFSAA+AATADGQHP KEGDEKIKM
Sbjct: 61  NLKLHSKQLRRTLRFLEEEKLVTRDHRRETAKGAKIFSAAIAATADGQHPGKEGDEKIKM 120

Query: 121 HTHSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180
           HTHSYCSLDYAQ+YDVVRYR+HRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS
Sbjct: 121 HTHSYCSLDYAQVYDVVRYRLHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180

Query: 181 LEDEYFHCENCNGELVAESDKLAAAQGVDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQL 240
           LEDEYFHCENCNGELVAESDKLAAAQG DGDDNARKRRHEKLKDMLQKMEVQLKPLMEQL
Sbjct: 181 LEDEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQL 240

Query: 241 SRVKDLPVPEFGTLLAWEARASAAARAANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVE 300
           SRVKDLPVPEFGTLLAWEARASAAAR ANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVE
Sbjct: 241 SRVKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVE 300

Query: 301 VAFSGAEGKGADVKSESENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQFS 360
           VAFSGAEGKG +VKSESENT LKVLPPWMIK+GMNLTKEQRGEVK +SKMDAGSASAQFS
Sbjct: 301 VAFSGAEGKGVNVKSESENTALKVLPPWMIKEGMNLTKEQRGEVKGDSKMDAGSASAQFS 360

Query: 361 DDKKSSANEDDKTNIQ----ASYYAAILKKQQELEEAAKGQQEFSSTEVTENVSSTNSSR 420
           DDKKSSAN+DDKTNIQ     +YYAAILKKQQELEEAAK Q+E SSTEVTEN+S TNSSR
Sbjct: 361 DDKKSSANDDDKTNIQDEYVKAYYAAILKKQQELEEAAKAQKELSSTEVTENLSYTNSSR 420

Query: 421 QVGMKAKREED-EEDDVEWEEAPIGGSANENYKVGDLNVEASASVEDEEEDDVDWEEG 474
           QVGMKAKREED EEDD+EWEEAPI G+ANENYKVGDLNVEASASVEDEEEDDVDWEEG
Sbjct: 421 QVGMKAKREEDEEEDDIEWEEAPIEGNANENYKVGDLNVEASASVEDEEEDDVDWEEG 478

[Close]
BLAST of Spg022527 vs. ExPASy TrEMBL
Match: A0A6J1JA07 (Transcription initiation factor IIE subunit alpha OS=Cucurbita maxima OX=3661 GN=LOC111484895 PE=3 SV=1)

HSP 1 Score: 857.1 bits (2213), Expect = 3.6e-245
Identity = 447/478 (93.51%), Postives = 461/478 (96.44%), Query Frame = 0 

Query: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60
           MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK
Sbjct: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60

Query: 61  NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQHPVKEGDEKIKM 120
           NLKLHSKQLRRTLRFLEEEKL+TRDHRRETAKGAKIFSAAVAATADGQHP KEGDEKIKM
Sbjct: 61  NLKLHSKQLRRTLRFLEEEKLVTRDHRRETAKGAKIFSAAVAATADGQHPGKEGDEKIKM 120

Query: 121 HTHSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180
           HTHSYCSLDYAQ+YDVVRYR+HRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS
Sbjct: 121 HTHSYCSLDYAQVYDVVRYRLHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180

Query: 181 LEDEYFHCENCNGELVAESDKLAAAQGVDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQL 240
           LEDEYFHCENCNGELVAESDKLAAAQG DGDDNARKRRHEKLKDMLQKMEVQLKPLMEQL
Sbjct: 181 LEDEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQL 240

Query: 241 SRVKDLPVPEFGTLLAWEARASAAARAANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVE 300
           SRVKDLPVPEFGTLLAWEARASAAAR ANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVE
Sbjct: 241 SRVKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVE 300

Query: 301 VAFSGAEGKGADVKSESENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQFS 360
           VAFSGAE KG +VKSESENT LKVLPPWMIK+GMNLTKEQRGEVK ESKMDAGSASAQFS
Sbjct: 301 VAFSGAESKGVNVKSESENTALKVLPPWMIKEGMNLTKEQRGEVKGESKMDAGSASAQFS 360

Query: 361 DDKKSSANEDDKTNIQ----ASYYAAILKKQQELEEAAKGQQEFSSTEVTENVSSTNSSR 420
           DDKKSSAN+DDKTNIQ     +YYAAILKKQQELEEAAKGQ+E S TE+T+N+S TN+SR
Sbjct: 361 DDKKSSANDDDKTNIQDEYVKAYYAAILKKQQELEEAAKGQKELSRTEITDNLSYTNASR 420

Query: 421 QVGMKAKREED-EEDDVEWEEAPIGGSANENYKVGDLNVEASASVEDEEEDDVDWEEG 474
           QVGMKAKREED EEDD+EWEEAPI G+ANENYKVGDLNVEASASVEDEEEDDVDWEEG
Sbjct: 421 QVGMKAKREEDEEEDDIEWEEAPIEGNANENYKVGDLNVEASASVEDEEEDDVDWEEG 478

[Close]
BLAST of Spg022527 vs. ExPASy TrEMBL
Match: A0A6J1BZ86 (Transcription initiation factor IIE subunit alpha OS=Momordica charantia OX=3673 GN=LOC111007077 PE=3 SV=1)

HSP 1 Score: 855.1 bits (2208), Expect = 1.4e-244
Identity = 451/478 (94.35%), Postives = 460/478 (96.23%), Query Frame = 0 

Query: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60
           MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK
Sbjct: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60

Query: 61  NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQHPVKEGDEKIKM 120
           NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQ P KEGDEKIKM
Sbjct: 61  NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQQPGKEGDEKIKM 120

Query: 121 HTHSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180
           HTHSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS
Sbjct: 121 HTHSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180

Query: 181 LEDEYFHCENCNGELVAESDKLAAAQGVDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQL 240
           LEDEYFHCENCNGELVAESDKLAAAQG DGDDNARKRRHEKLKDMLQKMEVQLKPLMEQL
Sbjct: 181 LEDEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQL 240

Query: 241 SRVKDLPVPEFGTLLAWEARASAAARAANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVE 300
           SRVKDLPVPEFGTLLAWEARASAAAR ANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVE
Sbjct: 241 SRVKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVE 300

Query: 301 VAFSGAEGKGADVKSESENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQFS 360
           VAFSGAEGKG DVKSE+ENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSA AQFS
Sbjct: 301 VAFSGAEGKGVDVKSETENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSA-AQFS 360

Query: 361 DDKKSSANEDDKTNIQ----ASYYAAILKKQQELEEAAKGQQEFSSTEVTENVSSTNSSR 420
           DDKKSSAN+DDKTN Q     +YYAAILKKQQELEE+AK  +E SSTEVTE+VS+ NSSR
Sbjct: 361 DDKKSSANDDDKTNFQDEYVKAYYAAILKKQQELEESAKRPEELSSTEVTESVSNANSSR 420

Query: 421 QVGMKAKREEDEE-DDVEWEEAPIGGSANENYKVGDLNVEASASVEDEEEDDVDWEEG 474
           QVGMKAKRE DEE DD+EWEEAPIGG+ANENYKVGDLNVEASASVEDEEEDDVDWEEG
Sbjct: 421 QVGMKAKREGDEEDDDIEWEEAPIGGNANENYKVGDLNVEASASVEDEEEDDVDWEEG 477

[Close]
BLAST of Spg022527 vs. ExPASy TrEMBL
Match: A0A5D3DYI4 (Transcription initiation factor IIE subunit alpha OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00230 PE=3 SV=1)

HSP 1 Score: 837.0 bits (2161), Expect = 3.8e-239
Identity = 441/478 (92.26%), Postives = 456/478 (95.40%), Query Frame = 0 

Query: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60
           MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK
Sbjct: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60

Query: 61  NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQHPVKEGDEKIKM 120
           NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQH  KEGDEKIKM
Sbjct: 61  NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQHQTKEGDEKIKM 120

Query: 121 HTHSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180
           HTHSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS
Sbjct: 121 HTHSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180

Query: 181 LEDEYFHCENCNGELVAESDKLAAAQGVDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQL 240
            EDEYFHCENCNGELVAESDKLAA QG DGDDNARKRRHEKLKDMLQKME QLKPL+EQL
Sbjct: 181 FEDEYFHCENCNGELVAESDKLAAVQGGDGDDNARKRRHEKLKDMLQKMEAQLKPLVEQL 240

Query: 241 SRVKDLPVPEFGTLLAWEARASAAARAANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVE 300
           +RVKDLPVPEFGTLLAWEARASAAAR  NGDLNGNDPSKSSQ  GYGGTPMPFVGETKVE
Sbjct: 241 TRVKDLPVPEFGTLLAWEARASAAARGVNGDLNGNDPSKSSQ--GYGGTPMPFVGETKVE 300

Query: 301 VAFSGAEGKGADVKSESENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQFS 360
           VAFSGA+GKG DVKSES+NTGLKVLPPWMIK+GMNLTKEQRGEVK+ESK DAGSASAQFS
Sbjct: 301 VAFSGADGKGVDVKSESKNTGLKVLPPWMIKQGMNLTKEQRGEVKDESKTDAGSASAQFS 360

Query: 361 DDKKSSANEDDKTNIQ----ASYYAAILKKQQELEEAAKGQQEFSSTEVTENVSSTNSSR 420
           DDKKS AN+DDKTNIQ     +YYAAILKKQQELEEAAKGQQE SSTEVTE+V++TNSSR
Sbjct: 361 DDKKSLANDDDKTNIQDEYVKAYYAAILKKQQELEEAAKGQQELSSTEVTESVANTNSSR 420

Query: 421 QVGMKAKREE-DEEDDVEWEEAPIGGSANENYKVGDLNVEASASVEDEEEDDVDWEEG 474
           QVGMKAKREE D++DD+EWEEAPIGG+ANENYKV DLNVEASA VEDEEEDDVDWEEG
Sbjct: 421 QVGMKAKREEDDDDDDIEWEEAPIGGNANENYKV-DLNVEASALVEDEEEDDVDWEEG 475

[Close]
BLAST of Spg022527 vs. ExPASy TrEMBL
Match: A0A5A7UX12 (Transcription initiation factor IIE subunit alpha OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G003550 PE=3 SV=1)

HSP 1 Score: 836.6 bits (2160), Expect = 5.0e-239
Identity = 441/478 (92.26%), Postives = 456/478 (95.40%), Query Frame = 0 

Query: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60
           MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK
Sbjct: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60

Query: 61  NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQHPVKEGDEKIKM 120
           NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQH  KEGDEKIKM
Sbjct: 61  NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQHQTKEGDEKIKM 120

Query: 121 HTHSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180
           HTHSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS
Sbjct: 121 HTHSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180

Query: 181 LEDEYFHCENCNGELVAESDKLAAAQGVDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQL 240
            EDEYFHCENCNGELVAESDKLAA QG DGDDNARKRRHEKLKDMLQKME QLKPL+EQL
Sbjct: 181 FEDEYFHCENCNGELVAESDKLAAVQGGDGDDNARKRRHEKLKDMLQKMEAQLKPLVEQL 240

Query: 241 SRVKDLPVPEFGTLLAWEARASAAARAANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVE 300
           +RVKDLPVPEFGTLLAWEARASAAAR  NGDLNGNDPSKSSQ  GYGGTPMPFVGETKVE
Sbjct: 241 TRVKDLPVPEFGTLLAWEARASAAARGVNGDLNGNDPSKSSQ--GYGGTPMPFVGETKVE 300

Query: 301 VAFSGAEGKGADVKSESENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQFS 360
           VAFSGAEGKG DVKSES+NTGLKVLPPWMIK+GMNLTKEQRGEVK+ESK DAGSASAQFS
Sbjct: 301 VAFSGAEGKGVDVKSESKNTGLKVLPPWMIKQGMNLTKEQRGEVKDESKTDAGSASAQFS 360

Query: 361 DDKKSSANEDDKTNIQ----ASYYAAILKKQQELEEAAKGQQEFSSTEVTENVSSTNSSR 420
           DDKKS AN+DDKTNIQ     +YYAAILKKQQELEEAAKGQQE SSTEVTE+V++TNSSR
Sbjct: 361 DDKKSLANDDDKTNIQDEYVKAYYAAILKKQQELEEAAKGQQELSSTEVTESVANTNSSR 420

Query: 421 QVGMKAKREE-DEEDDVEWEEAPIGGSANENYKVGDLNVEASASVEDEEEDDVDWEEG 474
           +VGMKAKREE D++DD+EWEEAPIGG+ANENYKV DLNVEASA VEDEEEDDVDWEEG
Sbjct: 421 RVGMKAKREEDDDDDDIEWEEAPIGGNANENYKV-DLNVEASALVEDEEEDDVDWEEG 475

[Close]
BLAST of Spg022527 vs. TAIR 10
Match: AT1G03280.1 (Transcription factor TFIIE, alpha subunit )

HSP 1 Score: 565.1 bits (1455), Expect = 5.4e-161
Identity = 319/483 (66.05%), Postives = 384/483 (79.50%), Query Frame = 0 

Query: 3   IEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNL 62
           ++PF +LV+L ARAFYDD TTK DNQ K+ RSDNRGIA VVLDAL RRQWVREEDLAK+L
Sbjct: 13  LQPFVKLVRLVARAFYDDYTTKSDNQQKSARSDNRGIAAVVLDALARRQWVREEDLAKDL 72

Query: 63  KLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQHPVKEGDEKIKMHT 122
           +LH+KQLR+ +R  EEEKLI RDHR+ETAKGAK++SAAVAAT DG+      ++K+K+HT
Sbjct: 73  QLHAKQLRKIIRLFEEEKLIMRDHRKETAKGAKMYSAAVAATTDGR-----AEDKVKLHT 132

Query: 123 HSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLE 182
           HSYC LDYAQI DVVR+R+HRMKK+LKDELEDKNTVQEY CPNC R+Y ALDALRLIS+ 
Sbjct: 133 HSYCCLDYAQICDVVRFRLHRMKKRLKDELEDKNTVQEYGCPNCQRKYNALDALRLISMV 192

Query: 183 DEYFHCENCNGELVAESDKLAAAQGVDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSR 242
           D+ FHCENCNGELV E +KL + + VDGDDNAR+RR E LK+MLQK+EVQ+KPLM+QL+R
Sbjct: 193 DDSFHCENCNGELVVECNKLTSEEVVDGDDNARRRRRENLKNMLQKLEVQMKPLMDQLNR 252

Query: 243 VKDLPVPEFGTLLAWEARASAAARAANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVEVA 302
           VKDLP+PEFG+ LAWEARA+ AAR  NGDLN NDP + SQG GYG TPMPF+GETKVEV 
Sbjct: 253 VKDLPIPEFGSFLAWEARAAMAAR-ENGDLNPNDPLR-SQG-GYGSTPMPFLGETKVEVN 312

Query: 303 FSGAEGKGADVKSESENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGS-ASAQFSD 362
                    DVKS+  ++ LKVLPPWMIK+GMNLT+EQRGE+++E+K+D G+ A+A+ SD
Sbjct: 313 LGDG---NEDVKSKGGDSSLKVLPPWMIKEGMNLTEEQRGEMRQEAKVDGGAGAAAKLSD 372

Query: 363 DKKSSANEDDKTNIQASY---YAAILKKQQELEEAAKGQQEFSSTEVTENVSS--TNSSR 422
           DKKS+    D+ +++  Y   Y A L KQQEL  AA+  Q+ S+ E T  + S    S R
Sbjct: 373 DKKSAIGNGDEKDLKDEYLKAYYAELMKQQEL--AARRNQQESAGEPTSGIQSGTVYSGR 432

Query: 423 QVGMKAKREEDEEDD---VEWEE-APIGGSANENYKVGDLNVEASAS--VEDEEEDDVDW 474
           QV MKAKREEDE++D   VEWEE AP+  +AN NYKV DLNVEA AS   E+EEEDDVDW
Sbjct: 433 QVSMKAKREEDEDEDEEEVEWEEKAPV--TANGNYKV-DLNVEAEASGGEEEEEEDDVDW 479

[Close]
BLAST of Spg022527 vs. TAIR 10
Match: AT4G20340.1 (Transcription factor TFIIE, alpha subunit )

HSP 1 Score: 464.9 bits (1195), Expect = 7.6e-131
Identity = 269/479 (56.16%), Postives = 345/479 (72.03%), Query Frame = 0 

Query: 3   IEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNL 62
           +EPF +LV+L  R FYD+ T + DNQ K+ + + +G AV+VLDALTRRQWVREEDLAK +
Sbjct: 14  LEPFVKLVRLLVRIFYDNYTPESDNQQKSVK-NVKGSAVIVLDALTRRQWVREEDLAKEV 73

Query: 63  KLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQHPVKEGDEKIKMHT 122
           K ++K+LR+ +R  EE+K + R HR+ETAK AK++S AV  T DG+      ++ +K HT
Sbjct: 74  KRNAKELRKLIRHFEEQKFVMRYHRKETAKRAKMYSYAVGGTTDGR-----AEDNVKFHT 133

Query: 123 HSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLE 182
           HSYC LDYAQIYD+VRY++HR+KKK KDELED+NTVQEY CPNC R+Y ALDALRLIS+E
Sbjct: 134 HSYCCLDYAQIYDIVRYKLHRLKKKFKDELEDRNTVQEYGCPNCKRKYNALDALRLISME 193

Query: 183 DEYFHCENCNGELVAESDKLAAAQGVDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSR 242
           D+ FHCENCNGELV E +KL + + VD  DNAR+R+ EK+K  LQ +E +LKPLME ++R
Sbjct: 194 DDSFHCENCNGELVMECNKLISEEVVDRGDNARRRQREKVKVWLQDLEGELKPLMELINR 253

Query: 243 VKDLPVPEFGTLLAWEARASAAARAANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVEVA 302
           VKDLP P F    AWEARA+ AAR  NGD N +DPS+S    GYG TPMPF+GETKVEV 
Sbjct: 254 VKDLPFPAFEPFPAWEARAAKAAR-ENGDFNPDDPSRSLG--GYGSTPMPFLGETKVEVN 313

Query: 303 FSGAEGKGADVKSESENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQFSDD 362
               EG   DV S   ++ LK+LPPWMIK+GM LT+EQRGE+++E+ +D    +A+ SDD
Sbjct: 314 L--GEG-NEDVTSTGGDSSLKMLPPWMIKQGMKLTEEQRGEMRQEANVD--GEAAKLSDD 373

Query: 363 KKS-----SANEDDKTNIQASYYAAILKKQQELEEAAKGQQEFSSTEVTENVSSTNSSRQ 422
           KKS       N+D K     +YYAAI+++Q+   +  + +    ST      ++T S RQ
Sbjct: 374 KKSVMENGDDNKDLKDEYLKAYYAAIMEQQKLAAKLNEQESAGESTTTDIESATTYSDRQ 433

Query: 423 VGMKAKREEDEEDDVEWEEAPIGGSANENYKVGDLNVEASASVEDE---EEDDVDWEEG 474
           VGMK+KREE EE+DVEWEE     +AN NYKV DLNVEA  + E E   E+DD+DWEEG
Sbjct: 434 VGMKSKREE-EEEDVEWEEG-ASVAANGNYKV-DLNVEAEEAEEKEDGDEDDDIDWEEG 475

[Close]
BLAST of Spg022527 vs. TAIR 10
Match: AT4G20810.1 (transcription initiation factor IIE (TFIIE) alpha subunit family protein / general transcription factor TFIIE family protein )

HSP 1 Score: 382.5 bits (981), Expect = 5.0e-106
Identity = 236/467 (50.54%), Postives = 320/467 (68.52%), Query Frame = 0 

Query: 9   LVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNLKLHSKQ 68
           +VKL A+ FYD+ T K +NQ K+ ++ + GIAV+VLDALTRRQWVREEDLAK LKL++KQ
Sbjct: 1   MVKLVAKTFYDNYTPKNNNQKKSAKNGSGGIAVLVLDALTRRQWVREEDLAKELKLNTKQ 60

Query: 69  LRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQHPVKEGDEKIKMHTHSYCSL 128
           LR  LR+ EE++ I R HR+E +          +AT +G+     G++K+K+H +SYC L
Sbjct: 61  LRTILRYFEEQQFIMRVHRKEKS----------SATTNGR-----GEDKVKVHMYSYCCL 120

Query: 129 DYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLEDEYFHC 188
           DY+QIYDV+RY++HRMKK+ KD LEDK+ VQEY CPNC R+               +FHC
Sbjct: 121 DYSQIYDVIRYKLHRMKKEFKDVLEDKDNVQEYGCPNCKRKI--------------FFHC 180

Query: 189 ENCNGELVAESDKLAAAQ-GVDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSRVKDLP 248
           ENCNGELV E +KL + +  VDG DN R RR + LKD+LQ MEV+LKPLM+ ++R+KDLP
Sbjct: 181 ENCNGELVMECNKLTSEEVVVDGSDNPRSRR-DHLKDLLQNMEVRLKPLMDHINRIKDLP 240

Query: 249 VPEFGTLLAWEARASAAARAANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVEVAFSGAE 308
           VP F +  AWE R + AAR  NGDLN +D  +  QG GYG TPMPF+GET++EV   G E
Sbjct: 241 VPSFESFPAWETRVAKAAR-ENGDLNPDDTLR-PQG-GYGSTPMPFLGETEIEVNL-GEE 300

Query: 309 GKGADVKSE--SENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQFSDDKKS 368
            +  DVKS+   +++  K+ P W+IKKGMNL+ EQRGE++ E+K D G +S +  DD ++
Sbjct: 301 NE--DVKSDEVGDSSRRKLTPSWLIKKGMNLSDEQRGEIRHEAKADDGGSSMENGDDDRN 360

Query: 369 SANEDDKTNIQASYYAAILKKQQELEEAAKGQQEFSSTEVTENVSSTNSSRQVGMKAKRE 428
             +E  K     +YYAAIL ++QEL E    Q+          +++++S RQVGMK+KRE
Sbjct: 361 LKDEYLK-----AYYAAIL-EEQELAEKLNQQESAGKVTTDIELATSSSDRQVGMKSKRE 415

Query: 429 EDEEDDVEWEEAPIGGSANENYKVGDLNVEASASVEDEEEDDVDWEE 473
           E+EE     EEA +  +AN NYKV DLNVEA  +  +++E+DVDW+E
Sbjct: 421 EEEE-----EEASV--AANGNYKV-DLNVEAEEA--EQDENDVDWQE 415

The following BLAST results are available for this feature:
BLAST of Spg022527 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038880289.13.8e-24995.39general transcription factor IIE subunit 1 isoform X1 [Benincasa hispida][more]
XP_038880290.14.6e-24795.18general transcription factor IIE subunit 1 isoform X2 [Benincasa hispida][more]
XP_023515738.16.7e-24693.93general transcription factor IIE subunit 1-like [Cucurbita pepo subsp. pepo] >XP... [more]
XP_022922068.18.7e-24693.93general transcription factor IIE subunit 1-like [Cucurbita moschata] >KAG6589563... [more]
XP_022987307.17.4e-24593.51general transcription factor IIE subunit 1-like [Cucurbita maxima][more]
BLAST of Spg022527 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q557M82.7e-1623.50General transcription factor IIE subunit 1 OS=Dictyostelium discoideum OX=44689 ... [more]
P361007.9e-0822.43Transcription initiation factor IIE subunit alpha OS=Saccharomyces cerevisiae (s... [more]
Q5R8H51.0e-0723.00General transcription factor IIE subunit 1 OS=Pongo abelii OX=9601 GN=GTF2E1 PE=... [more]
P290833.0e-0722.25General transcription factor IIE subunit 1 OS=Homo sapiens OX=9606 GN=GTF2E1 PE=... [more]
A6QLI82.0e-0623.02General transcription factor IIE subunit 1 OS=Bos taurus OX=9913 GN=GTF2E1 PE=2 ... [more]
BLAST of Spg022527 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1E5J24.2e-24693.93Transcription initiation factor IIE subunit alpha OS=Cucurbita moschata OX=3662 ... [more]
A0A6J1JA073.6e-24593.51Transcription initiation factor IIE subunit alpha OS=Cucurbita maxima OX=3661 GN... [more]
A0A6J1BZ861.4e-24494.35Transcription initiation factor IIE subunit alpha OS=Momordica charantia OX=3673... [more]
A0A5D3DYI43.8e-23992.26Transcription initiation factor IIE subunit alpha OS=Cucumis melo var. makuwa OX... [more]
A0A5A7UX125.0e-23992.26Transcription initiation factor IIE subunit alpha OS=Cucumis melo var. makuwa OX... [more]
BLAST of Spg022527 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G03280.15.4e-16166.05Transcription factor TFIIE, alpha subunit [more]
AT4G20340.17.6e-13156.16Transcription factor TFIIE, alpha subunit [more]
AT4G20810.15.0e-10650.54transcription initiation factor IIE (TFIIE) alpha subunit family protein / gener... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 59..79
NoneNo IPR availableCOILSCoilCoilcoord: 219..239
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 391..473
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 336..350
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 333..372
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 457..473
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 394..418
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 268..289
NoneNo IPR availableSUPERFAMILY57783Zinc beta-ribboncoord: 147..200
IPR002853Transcription initiation factor IIE subunit alpha, N-terminalSMARTSM00531tfiie3coord: 38..209
e-value: 9.9E-63
score: 224.4
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 147..206
e-value: 1.5E-8
score: 36.0
IPR024550TFIIEalpha/SarR/Rpc3 HTH domainPFAMPF02002TFIIE_alphacoord: 40..93
e-value: 3.3E-7
score: 30.1
IPR039997Transcription factor EPANTHERPTHR13097TRANSCRIPTION INITIATION FACTOR IIE, ALPHA SUBUNITcoord: 5..453
IPR017919Transcription factor TFE/TFIIEalpha HTH domainPROSITEPS51344HTH_TFE_IIEcoord: 6..138
score: 34.170559
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 42..127

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg022527.1Spg022527.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001113 transcription open complex formation at RNA polymerase II promoter
biological_process GO:0006367 transcription initiation from RNA polymerase II promoter
cellular_component GO:0005673 transcription factor TFIIE complex