Spg022456 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg022456
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	5-formyltetrahydrofolate cyclo-ligase
Location	scaffold2: 5223591 .. 5227695 (-)
RNA-Seq Expression	Spg022456
Synteny	Spg022456

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTACAAGATATTGGGCACCATGAGAACGACTCTTCACCAACCTCTTTAGCCAAAGACTCGATCTCATCCAACACATTCTTAGTTTTCGAAAGGTCAGTCGATTGGGAATAAGTTCAAACGTCTCTAGTTCCTCTAAATTATAAAATTATTTTGTTCTTCATATTTAACCTTCTTTTAAAAAGACAAAAGAAAGAACATAGATAGAAATTAAGAAAACACAGCATTGGTCGGTGTTTTATTTCATCAATCACTGTAAAAAAAAAAAGAAAAAAGAAATACAATGTTGAGATGGTAATAAATGTTTTATCCACCATGAAAGTTCAAAATGTTTGAGACATTATTATTGTTTTTTTTTTTTTGCACCATAACAAACATAGATATACGAAAACTTGGATTTTAACTTTGAGTACTTTAATATTAAGGTGTGTTGATGCTGCTAAAGTGAGTTTAGCTCAGCGGTAATTGGCATGTACCCTCAACCATAAGGTTAGAGGTTTGAATCCTCCCACCCCCAAATGTTGATGTACTCAACAAAAAAAAAAAAAAAAAAGGTGTGTTGATGCTGGTAAAATTCACTTTTGAAACTTATATTTGATTTTATTTTGACAGAGCCACACTATTCATTTTACATTTAAAACGGCACAATCTTAACCTCAAACAATCTCCATATGATTTGAAAGGGTCCAAATCGTGGAATTGTAAGCACATTGTTGTTGTTTGTGATCGGAGCATTTTGGATTTGATGATTAAATCTCGTCTCCTCCAAGGAAATGAGACAAATTCACCCGTGAGTGGATTTCAAAATTCGACCTCTGCTTTTAGATTGACGAACCAGCGAAGTCCAGCCGGAGTGCACGTGGGTCGGAGCCCAAAAGCCAGAAGGTGGGCAATTTCCGAAGCCCCAATGGTCCGAAACTATAAACTATTACCCATTGCAGCAAATTCACTGTTCAATCGCCTCCCGCCTTCAGCTTTCCAACAAGCACTTCCTCCATTATCGTTTCGCTCATCTGGGTCCCCGAAAATGGCGAGTAACGAGAGCAGCAAACCAGAAAACGAAGACCAATTGAAGGCCATATTTGAGCAGAAACGGGTTCTTCGATCCAGTGTTCGAAAAGCCCTCAAAGCCATGGATCCCTCCCTCAGATCCCAGGAAGGTACCAATTGCTTACATATCTTAATCGATTAACTTCGCTGTACTGAATATGTTAGTTCGAGGCATAGAAAAATTGATTGGATATCGTTTGATGATGTTGATGAATCGAGAGAATGTGCTATATTCTGTTGCTCCAATTTCATTGGCCCTCCTACTTGTTTCTCAGACAATGCAATTCAGAGTATAGTTTTGGAAGCTCCTTGGTTTAAATCCAGTCAGAGATTATGTGCTTATGTAAGTTGCAGTGCCTTAAGAGAAGTTGATACTTCGAGACTATTATCAGAGACTCTGCAACACCCACCAAAAGGTGAGTTCTTAATCGTACTAGCCACTTTCTAATTCTATCTGAAGTTTCTAGGATCAGTTTGGCAACATTGACTTAATCAAGTCAGTAAAGCTCAATGGAAGATTTTATATTTGAAGTTTTACATTAAATAACAGAATCACAGTTTTACCAACTCAATCAGATTCTTTAGTCTAATTCAACCAATTAGGTTAGGAGAATATGATCGTATACTTGGAACTGGTTTAAGTTTAAAACAGAGACTATTTGATATGGTGGATCTGAATGCAGAGTACATATGTGGAAGTATTGTTACTCATTATATTAGTAAGTAATTTTTTGAACTCGATATTGGTTTTACGATACAAGATACTGCTTTTCTAGCTAGCATTCTCTTATGCAGCTTTGTGAATGTTGACATTTTTCTTTTTATTTGAAGATGGTTATTCGAAGAAAATTTATGTCCCGCGTGTGGAGGACAAGAATAGTCACATGCGGATGTTCAATATTTCACGTATGGACGATCTTATTGCAAATTCAATGAATATCTTAGAACCAGCTCCAGTGGATGGTGATGGAAATGAACGTGAAGATGGTAACTAGATACTTCACCCCATCTCTTTTCATTTTATATTGTTAGTACCCAGCTTAATGATCGTACTTGTGGTCTATATGATTAATCTATAGTCTGCTATATATGGCACTGGGATAATGATATACTTGACTTATTATATATAAAACCAGCATATAACAGCGTTTTAAGATCGAACCATTTATGTTTATATCATTACTGATATTATTGTTTTCTGGCCTGGTCTCCTCTAATGCAAGGATGGCTTTGCAAATAACTCTAGACAATTGTTTCCTTACCATCGGAGACCAAACATATTTTGTTTCAAACAGCAGAAATCCAGGTCATTCCTGTCTTGCATGGTCAACCGATCTATTAATTCTTCAATGTGAGCCAGAATTTAACTTTCGGTGGTTTGCATCTTACAGATTCCATCTACCAGTATCCCCTACTAAATACTTGTCTACATTTCCAACCCACCACCAGTAGTTTTCTGGACTTTCTTTGAGCTTAACGAGAGGTCATGGAACTGTTGTTTCATTCTTTCAATATTTTTTAATACCCAGAAATCCTTAACTTATTTCGTTAAATTCCTTTTACAGACTTGTTAAGTTTTCCTGGAATATATTACTCTATCGATACCAAATGAACCATGACTGTTTTAAGAGAGAAATAATTGGTCTAACATAGTTTTTTATGAGAGAAATGAAGAGCACAAATGAGGTAGGTGTGCATCATACTCGACCTTTCAACTGAGCTGTACCATGAATCTTGTCTGTGATACACATGCTAATAGCAATAGCAAATATAAAATTTTATGGCATTTTCCCTTCAGGGTGGGGAGTGCTTAGCCATGAGTCTGCTTTGGCATATATTATAACAGTACTCTTATGGTGATAATTGATATCAACTAAACATTATTGTTATCCATACGGTCTCTCTCTCTCTCCCTTTTTTTTTTTTTTTTTTTTTTCCTGTTGTCAATGAAGAAATGATTTGACATCATCTGTCTCTGCATCTTGAACGTAACACCACTGATATTTAAACATCTATGTTTTGCAGTTATGCAGACAAAAGACCCCATTGATTTATTTCTCTTACCAGGTGGGATATTCATATCTATTTGTTGATTGTTTAGGGTTTATTTATAGGTTAGTAGCTGACATGAAATCACTTGTTAATATTTTGATGATAATTTCTGCCAGGTCTAGCATTTGATAAATCAGGAAGACGCCTAGGCCGAGGGGGAGGGTATGGATTTGCATTTAGCCTTTTTGAGGTTTCTTTCTGCTGCTGTTATTACTGAATTTTTAAGACATTTGGAGCTGATTTGTGAAGTTTAAACTAGATACAGTAACTCCCAAATCATGATAGTAAAATCTCTCCTCTATGTTTTTTTTTTCTTTGCTTGTTTAGATTTTGGCCCATATTCAATCTGTATATCTTATACATCCTACTCCAAATGAATCTTATCCTATAATTGTCATGAAAACAGTTATTATGATACCTTCCTAAAGAACTACCAAGAGCTTGCAAAGGCACGGAATTGGAAGCAGCCCCTCCTTGGTATGTGTGTAAGCTCTCTCCTTTGTAGTAGAACCTCGCCTTAGTTTGCTTTTTCAGCTCACCTCGTTTCCCTGTAACTTCACTTAATTCAGTTAGCATGATTTTGCAGTTGCCCTGTCCTATTCTGTACAGATAATGGATGAAGGAGTTATACCACTCACTCCAAATGATGTTTTAGTTGATGCTCTCGTATCACCATCGGGTGTGATTCCCACCAGCTCAGCTGGATTGGACAAGTAATTTCTTTCATCTGAGAAGTCTAAGAATCACTTGTCATTCCAATTTTTTCTTTGGTTTCTTACTTCCTACTTGTCATATCCTCGCAGAATGAAAGTATGATATGGTGCTCAGAGAATCAATCTCTAACGATGCCTTCTCTGATGAAATCCTTGACAATCTGGAGCTTGGAATCCTTGAAGCTGTTAAAATTATATGTACGCCTTTTCCCTCTCTTTTCAAATGGCACCTATTTCAGATAGGCCATCATGAGGAAGTTATGGTTCCTCGGCCATTATTTTCTTCTTAG

mRNA sequence

ATGTTACAAGATATTGGGCACCATGAGAACGACTCTTCACCAACCTCTTTAGCCAAAGACTCGATCTCATCCAACACATTCTTAGTTTTCGAAAGGTGTGTTGATGCTGCTAAAGTGAGTTTAGCTCAGCGAGCCACACTATTCATTTTACATTTAAAACGGCACAATCTTAACCTCAAACAATCTCCATATGATTTGAAAGGGTCCAAATCGTGGAATTGTAAGCACATTGTTGTTGTTTGTGATCGGAGCATTTTGGATTTGATGATTAAATCTCGTCTCCTCCAAGGAAATGAGACAAATTCACCCGTGAGTGGATTTCAAAATTCGACCTCTGCTTTTAGATTGACGAACCAGCGAAGTCCAGCCGGAGTGCACGTGGGTCGGAGCCCAAAAGCCAGAAGGTGGGCAATTTCCGAAGCCCCAATGGTCCGAAACTATAAACTATTACCCATTGCAGCAAATTCACTGTTCAATCGCCTCCCGCCTTCAGCTTTCCAACAAGCACTTCCTCCATTATCGTTTCGCTCATCTGGGTCCCCGAAAATGGCGAGTAACGAGAGCAGCAAACCAGAAAACGAAGACCAATTGAAGGCCATATTTGAGCAGAAACGGGTTCTTCGATCCAGTGTTCGAAAAGCCCTCAAAGCCATGGATCCCTCCCTCAGATCCCAGGAAGACAATGCAATTCAGAGTATAGTTTTGGAAGCTCCTTGGTTTAAATCCAGTCAGAGATTATGTGCTTATGTAAGTTGCAGTGCCTTAAGAGAAGTTGATACTTCGAGACTATTATCAGAGACTCTGCAACACCCACCAAAAGATGGTTATTCGAAGAAAATTTATGTCCCGCGTGTGGAGGACAAGAATAGTCACATGCGGATGTTCAATATTTCACGTATGGACGATCTTATTGCAAATTCAATGAATATCTTAGAACCAGCTCCAGTGGATGGTGATGGAAATGAACGTGAAGATGTTATGCAGACAAAAGACCCCATTGATTTATTTCTCTTACCAGGTCTAGCATTTGATAAATCAGGAAGACGCCTAGGCCGAGGGGGAGGTTATTATGATACCTTCCTAAAGAACTACCAAGAGCTTGCAAAGGCACGGAATTGGAAGCAGCCCCTCCTTGTTGCCCTGTCCTATTCTGTACAGATAATGGATGAAGGAGTTATACCACTCACTCCAAATGATGTTTTAGTTGATGCTCTCGTATCACCATCGGGTGTGATTCCCACCAGCTCAGCTGGATTGGACAAAGAATCAATCTCTAACGATGCCTTCTCTGATGAAATCCTTGACAATCTGGAGCTTGGAATCCTTGAAGCTGTTAAAATTATATGTACGCCTTTTCCCTCTCTTTTCAAATGGCACCTATTTCAGATAGGCCATCATGAGGAAGTTATGGTTCCTCGGCCATTATTTTCTTCTTAG

Coding sequence (CDS)

ATGTTACAAGATATTGGGCACCATGAGAACGACTCTTCACCAACCTCTTTAGCCAAAGACTCGATCTCATCCAACACATTCTTAGTTTTCGAAAGGTGTGTTGATGCTGCTAAAGTGAGTTTAGCTCAGCGAGCCACACTATTCATTTTACATTTAAAACGGCACAATCTTAACCTCAAACAATCTCCATATGATTTGAAAGGGTCCAAATCGTGGAATTGTAAGCACATTGTTGTTGTTTGTGATCGGAGCATTTTGGATTTGATGATTAAATCTCGTCTCCTCCAAGGAAATGAGACAAATTCACCCGTGAGTGGATTTCAAAATTCGACCTCTGCTTTTAGATTGACGAACCAGCGAAGTCCAGCCGGAGTGCACGTGGGTCGGAGCCCAAAAGCCAGAAGGTGGGCAATTTCCGAAGCCCCAATGGTCCGAAACTATAAACTATTACCCATTGCAGCAAATTCACTGTTCAATCGCCTCCCGCCTTCAGCTTTCCAACAAGCACTTCCTCCATTATCGTTTCGCTCATCTGGGTCCCCGAAAATGGCGAGTAACGAGAGCAGCAAACCAGAAAACGAAGACCAATTGAAGGCCATATTTGAGCAGAAACGGGTTCTTCGATCCAGTGTTCGAAAAGCCCTCAAAGCCATGGATCCCTCCCTCAGATCCCAGGAAGACAATGCAATTCAGAGTATAGTTTTGGAAGCTCCTTGGTTTAAATCCAGTCAGAGATTATGTGCTTATGTAAGTTGCAGTGCCTTAAGAGAAGTTGATACTTCGAGACTATTATCAGAGACTCTGCAACACCCACCAAAAGATGGTTATTCGAAGAAAATTTATGTCCCGCGTGTGGAGGACAAGAATAGTCACATGCGGATGTTCAATATTTCACGTATGGACGATCTTATTGCAAATTCAATGAATATCTTAGAACCAGCTCCAGTGGATGGTGATGGAAATGAACGTGAAGATGTTATGCAGACAAAAGACCCCATTGATTTATTTCTCTTACCAGGTCTAGCATTTGATAAATCAGGAAGACGCCTAGGCCGAGGGGGAGGTTATTATGATACCTTCCTAAAGAACTACCAAGAGCTTGCAAAGGCACGGAATTGGAAGCAGCCCCTCCTTGTTGCCCTGTCCTATTCTGTACAGATAATGGATGAAGGAGTTATACCACTCACTCCAAATGATGTTTTAGTTGATGCTCTCGTATCACCATCGGGTGTGATTCCCACCAGCTCAGCTGGATTGGACAAAGAATCAATCTCTAACGATGCCTTCTCTGATGAAATCCTTGACAATCTGGAGCTTGGAATCCTTGAAGCTGTTAAAATTATATGTACGCCTTTTCCCTCTCTTTTCAAATGGCACCTATTTCAGATAGGCCATCATGAGGAAGTTATGGTTCCTCGGCCATTATTTTCTTCTTAG

Protein sequence

MLQDIGHHENDSSPTSLAKDSISSNTFLVFERCVDAAKVSLAQRATLFILHLKRHNLNLKQSPYDLKGSKSWNCKHIVVVCDRSILDLMIKSRLLQGNETNSPVSGFQNSTSAFRLTNQRSPAGVHVGRSPKARRWAISEAPMVRNYKLLPIAANSLFNRLPPSAFQQALPPLSFRSSGSPKMASNESSKPENEDQLKAIFEQKRVLRSSVRKALKAMDPSLRSQEDNAIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSETLQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVMQTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQIMDEGVIPLTPNDVLVDALVSPSGVIPTSSAGLDKESISNDAFSDEILDNLELGILEAVKIICTPFPSLFKWHLFQIGHHEEVMVPRPLFSS

Homology

[Close]

BLAST of Spg022456 vs. NCBI nr
Match: XP_022988398.1 (5-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 597.8 bits (1540), Expect = 8.1e-167
Identity = 306/336 (91.07%), Postives = 318/336 (94.64%), Query Frame = 0


Query: 89  MIKSRLLQGNETNSPVSGFQNSTSAFRLTNQRSPAGVHVGRSPKARRWAISEAPMVRNYK 148
           MIKS+LLQGN+TN PV G QNST+A RLTN RSPAGVHVGRSPKARR AISE+PMVRNYK
Sbjct: 1   MIKSQLLQGNKTNLPVHGLQNSTTASRLTNPRSPAGVHVGRSPKARRRAISESPMVRNYK 60

Query: 149 LLPIAANSLFNRLPPSAFQQALPPLSFRSSGSPKMASNESSKPENEDQLKAIFEQKRVLR 208
           LLPIAA+SLFNRLPPSAFQ AL PLS  SSGSPKMA NE  KPENE+QLKA+FEQKRV+R
Sbjct: 61  LLPIAASSLFNRLPPSAFQPALRPLSRCSSGSPKMARNEGIKPENEEQLKALFEQKRVIR 120

Query: 209 SSVRKALKAMDPSLRSQEDNAIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSETL 268
           SSVRKALKAMDPSLRSQEDNAIQ+IVLEAPWFKSSQRLCAYVSCSALREVDTSR+LSETL
Sbjct: 121 SSVRKALKAMDPSLRSQEDNAIQNIVLEAPWFKSSQRLCAYVSCSALREVDTSRILSETL 180

Query: 269 QHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVMQ 328
           QHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVMQ
Sbjct: 181 QHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVMQ 240

Query: 329 TKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQIM 388
           TKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAK RNWKQPLLVALSYSVQIM
Sbjct: 241 TKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQIM 300

Query: 389 DEGVIPLTPNDVLVDALVSPSGVIPTSSAGLDKESI 425
           DEG+IPLTPNDVLVD LVSPSGVIP SSAGLDK  +
Sbjct: 301 DEGIIPLTPNDVLVDVLVSPSGVIPISSAGLDKMKV 336

[Close]

BLAST of Spg022456 vs. NCBI nr
Match: XP_022988397.1 (5-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 590.9 bits (1522), Expect = 1.0e-164
Identity = 305/338 (90.24%), Postives = 317/338 (93.79%), Query Frame = 0


Query: 89  MIKSRLLQGNETNSPVSGFQNSTSAFRLTNQRSPAGVHVGRSPKARRWAISEAPMVRNYK 148
           MIKS+LLQGN+TN PV G QNST+A RLTN RSPAGVHVGRSPKARR AISE+PMVRNYK
Sbjct: 1   MIKSQLLQGNKTNLPVHGLQNSTTASRLTNPRSPAGVHVGRSPKARRRAISESPMVRNYK 60

Query: 149 LLPIAANSLFNRLPPSAFQQALPPLSFRSSGSPKMASNESSKPENEDQLKAIFEQKRVLR 208
           LLPIAA+SLFNRLPPSAFQ AL PLS  SSGSPKMA NE  KPENE+QLKA+FEQKRV+R
Sbjct: 61  LLPIAASSLFNRLPPSAFQPALRPLSRCSSGSPKMARNEGIKPENEEQLKALFEQKRVIR 120

Query: 209 SSVRKALKAMDPSLRSQEDNAIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSETL 268
           SSVRKALKAMDPSLRSQEDNAIQ+IVLEAPWFKSSQRLCAYVSCSALREVDTSR+LSETL
Sbjct: 121 SSVRKALKAMDPSLRSQEDNAIQNIVLEAPWFKSSQRLCAYVSCSALREVDTSRILSETL 180

Query: 269 QHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNERED--V 328
           QHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNERED   
Sbjct: 181 QHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDGNF 240

Query: 329 MQTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQ 388
           MQTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAK RNWKQPLLVALSYSVQ
Sbjct: 241 MQTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQ 300

Query: 389 IMDEGVIPLTPNDVLVDALVSPSGVIPTSSAGLDKESI 425
           IMDEG+IPLTPNDVLVD LVSPSGVIP SSAGLDK  +
Sbjct: 301 IMDEGIIPLTPNDVLVDVLVSPSGVIPISSAGLDKMKV 338

[Close]

BLAST of Spg022456 vs. NCBI nr
Match: KGN63636.2 (hypothetical protein Csa_013278 [Cucumis sativus])

HSP 1 Score: 590.1 bits (1520), Expect = 1.7e-164
Identity = 302/338 (89.35%), Postives = 318/338 (94.08%), Query Frame = 0


Query: 89  MIKSRLLQGNETNSPVSGFQNSTSAFRLTNQRSPAGVHVGRSPKARRWAISEAPMVRNYK 148
           M+K +LLQGN TN PV+GFQNST+ FRLTNQ SPAGVH+GRSPK ++WAISEAPM+RNYK
Sbjct: 1   MVKYQLLQGNRTNLPVNGFQNSTTGFRLTNQLSPAGVHLGRSPKPQKWAISEAPMLRNYK 60

Query: 149 -LLPIAANSLFNRLPPSAFQQALPPLSFRSSGSPKMASNESSKPENEDQLKAIFEQKRVL 208
            LLPIAANSLFN +  SAFQQ+LPPLS  SSG PKMA+NES KPENEDQLK IF+QKRVL
Sbjct: 61  PLLPIAANSLFNHIQSSAFQQSLPPLSRFSSGYPKMATNESIKPENEDQLKHIFDQKRVL 120

Query: 209 RSSVRKALKAMDPSLRSQEDNAIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSET 268
           RSSVRKALKAMDPS RSQEDN IQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSE 
Sbjct: 121 RSSVRKALKAMDPSFRSQEDNVIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSEI 180

Query: 269 LQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 328
           LQHPPKDG+SKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM
Sbjct: 181 LQHPPKDGHSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 240

Query: 329 QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQI 388
           QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQI
Sbjct: 241 QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQI 300

Query: 389 MDEGVIPLTPNDVLVDALVSPSGVIPTSSAGLDKESIS 426
           MDEG+IPLTPNDVLVDALVSPSGVIP SSAGLDK +I+
Sbjct: 301 MDEGIIPLTPNDVLVDALVSPSGVIPISSAGLDKYAIT 338

[Close]

BLAST of Spg022456 vs. NCBI nr
Match: XP_004138292.1 (5-formyltetrahydrofolate cyclo-ligase, mitochondrial [Cucumis sativus])

HSP 1 Score: 589.0 bits (1517), Expect = 3.8e-164
Identity = 301/337 (89.32%), Postives = 316/337 (93.77%), Query Frame = 0


Query: 89  MIKSRLLQGNETNSPVSGFQNSTSAFRLTNQRSPAGVHVGRSPKARRWAISEAPMVRNYK 148
           M+K +LLQGN TN PV+GFQNST+ FRLTNQ SPAGVH+GRSPK ++WAISEAPM+RNYK
Sbjct: 1   MVKYQLLQGNRTNLPVNGFQNSTTGFRLTNQLSPAGVHLGRSPKPQKWAISEAPMLRNYK 60

Query: 149 -LLPIAANSLFNRLPPSAFQQALPPLSFRSSGSPKMASNESSKPENEDQLKAIFEQKRVL 208
            LLPIAANSLFN +  SAFQQ+LPPLS  SSG PKMA+NES KPENEDQLK IF+QKRVL
Sbjct: 61  PLLPIAANSLFNHIQSSAFQQSLPPLSRFSSGYPKMATNESIKPENEDQLKHIFDQKRVL 120

Query: 209 RSSVRKALKAMDPSLRSQEDNAIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSET 268
           RSSVRKALKAMDPS RSQEDN IQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSE 
Sbjct: 121 RSSVRKALKAMDPSFRSQEDNVIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSEI 180

Query: 269 LQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 328
           LQHPPKDG+SKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM
Sbjct: 181 LQHPPKDGHSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 240

Query: 329 QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQI 388
           QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQI
Sbjct: 241 QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQI 300

Query: 389 MDEGVIPLTPNDVLVDALVSPSGVIPTSSAGLDKESI 425
           MDEG+IPLTPNDVLVDALVSPSGVIP SSAGLDK  +
Sbjct: 301 MDEGIIPLTPNDVLVDALVSPSGVIPISSAGLDKMKV 337

[Close]

BLAST of Spg022456 vs. NCBI nr
Match: XP_022921537.1 (5-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 586.6 bits (1511), Expect = 1.9e-163
Identity = 300/336 (89.29%), Postives = 315/336 (93.75%), Query Frame = 0


Query: 89  MIKSRLLQGNETNSPVSGFQNSTSAFRLTNQRSPAGVHVGRSPKARRWAISEAPMVRNYK 148
           MIKS+LLQGN+TN PV G QNST+A RLTN R+PAGVHVGRSPKARR A+SE+PMVRNYK
Sbjct: 1   MIKSQLLQGNKTNLPVHGLQNSTTASRLTNPRNPAGVHVGRSPKARRRAVSESPMVRNYK 60

Query: 149 LLPIAANSLFNRLPPSAFQQALPPLSFRSSGSPKMASNESSKPENEDQLKAIFEQKRVLR 208
           LLPIAA+S FNRLPP+AFQ AL PLS  SS SPKMA NE  KPENE+QLKA+FEQKRV+R
Sbjct: 61  LLPIAASSPFNRLPPTAFQPALRPLSRCSSRSPKMARNEGIKPENEEQLKALFEQKRVIR 120

Query: 209 SSVRKALKAMDPSLRSQEDNAIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSETL 268
           SSVRKALKAMDPSLRSQEDNAIQ+IVLEAPWFKSSQRLCAYVSCSALREVDTSR+LSETL
Sbjct: 121 SSVRKALKAMDPSLRSQEDNAIQNIVLEAPWFKSSQRLCAYVSCSALREVDTSRILSETL 180

Query: 269 QHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVMQ 328
           QHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 
Sbjct: 181 QHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVML 240

Query: 329 TKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQIM 388
           TKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAK RNWKQPLLVALSYSVQIM
Sbjct: 241 TKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQIM 300

Query: 389 DEGVIPLTPNDVLVDALVSPSGVIPTSSAGLDKESI 425
           DEG+IPLTPNDVLVD LVSPSGVIP SSAGLDK  +
Sbjct: 301 DEGIIPLTPNDVLVDVLVSPSGVIPISSAGLDKMKV 336

[Close]

BLAST of Spg022456 vs. ExPASy Swiss-Prot
Match: Q8L539 (5-formyltetrahydrofolate cyclo-ligase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=5FCL PE=1 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 6.0e-88
Identity = 159/239 (66.53%), Postives = 200/239 (83.68%), Query Frame = 0


Query: 175 FRSSGSPKMASNESSKPENEDQLKAIFEQKRVLRSSVRKALKAMDPSLRSQEDNAIQSIV 234
           FR S + +     S+  +N+++L +IF+QKRV+RS+VRK+LKAMDPSLR+Q+D AIQ  V
Sbjct: 31  FRLSPATRPIVAMSTTSKNQEELDSIFKQKRVVRSTVRKSLKAMDPSLRTQQDEAIQKTV 90

Query: 235 LEAPWFKSSQRLCAYVSCSALREVDTSRLLSETLQHPPKDGYSKKIYVPRVEDKNSHMRM 294
           LEAPWFKS + LCAY+SC +L EVDTS++LSE LQHP  +   KK+YVP VEDKNS+MRM
Sbjct: 91  LEAPWFKSCKGLCAYISCKSLNEVDTSKILSEILQHPDSN-TQKKLYVPWVEDKNSNMRM 150

Query: 295 FNISRMDDLIANSMNILEPAPVDGDGNEREDVMQTKDPIDLFLLPGLAFDKSGRRLGRGG 354
            +IS M+DL+ANSMNILEPAPVD  GN+REDV+Q  +PIDLF+LPGLAFD+ GRRLGRGG
Sbjct: 151 LHISHMEDLVANSMNILEPAPVDAQGNDREDVLQADEPIDLFILPGLAFDRCGRRLGRGG 210

Query: 355 GYYDTFLKNYQELAKARNWKQPLLVALSYSVQIMDEGVIPLTPNDVLVDALVSPSGVIP 414
           GYYDTFLK YQ+ AK + W+ PL+VALSYS QI+++G IP+TPNDVL+DALV+PSGV+P
Sbjct: 211 GYYDTFLKRYQDRAKEKGWRYPLMVALSYSPQILEDGSIPVTPNDVLIDALVTPSGVVP 268

[Close]

BLAST of Spg022456 vs. ExPASy Swiss-Prot
Match: P49914 (5-formyltetrahydrofolate cyclo-ligase OS=Homo sapiens OX=9606 GN=MTHFS PE=1 SV=2)

HSP 1 Score: 94.4 bits (233), Expect = 3.8e-18
Identity = 65/214 (30.37%), Postives = 112/214 (52.34%), Query Frame = 0


Query: 199 AIFEQKRVLRSSVRKALKAMDPSLRSQEDNAIQSIVLEAPWFKSSQRLCAYVSCSALREV 258
           A+   KR LR  +++ L+AM    R ++   +   V+    ++ S+R+  ++S     E+
Sbjct: 5   AVSSAKRSLRGELKQRLRAMSAEERLRQSRVLSQKVIAHSEYQKSKRISIFLSMQ--DEI 64

Query: 259 DTSRLLSETLQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDD---LIANSMNILEPAP 318
           +T  ++ +  Q        K  ++PR   +++HM M  I   ++   L   S NI +P  
Sbjct: 65  ETEEIIKDIFQR------GKICFIPRYRFQSNHMDMVRIESPEEISLLPKTSWNIPQP-- 124

Query: 319 VDGDGNEREDVMQTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLK---NYQELAKARN 378
             G+G+ RE+ + T   +DL  +PGL FDK G RLGRG GYYD +LK    +QE+     
Sbjct: 125 --GEGDVREEALST-GGLDLIFMPGLGFDKHGNRLGRGKGYYDAYLKRCLQHQEV----- 184

Query: 379 WKQPLLVALSYSVQIMDEGVIPLTPNDVLVDALV 407
             +P  +AL++  QI  +  +P+  ND+ VD ++
Sbjct: 185 --KPYTLALAFKEQICLQ--VPVNENDMKVDEVL 196

[Close]

BLAST of Spg022456 vs. ExPASy Swiss-Prot
Match: Q9D110 (5-formyltetrahydrofolate cyclo-ligase OS=Mus musculus OX=10090 GN=Mthfs PE=2 SV=2)

HSP 1 Score: 90.9 bits (224), Expect = 4.2e-17
Identity = 64/209 (30.62%), Postives = 115/209 (55.02%), Query Frame = 0


Query: 204 KRVLRSSVRKALKAMDPSLRSQEDNAIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRL 263
           KR LR+ +++ L+A+    R ++   +   V+    +++S+R+  ++S     EV+T  +
Sbjct: 10  KRGLRAELKQRLRALSAEERLRQSLLLTQKVIAHNQYQNSKRISIFLSMQ--DEVETEVI 69

Query: 264 LSETLQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDD---LIANSMNILEPAPVDGDG 323
           + +  +        K  ++PR + +++HM M  ++  ++   L   S NI +P    G+G
Sbjct: 70  IKDIFKQ------GKICFIPRYQFQSNHMDMVRLTSSEEIALLPKTSWNIHQP----GEG 129

Query: 324 NEREDVMQTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLK---NYQELAKARNWKQPL 383
           + RE+ + T   +DL  LPGL FDK G RLGRG GYYDT+LK    +QE+       +P 
Sbjct: 130 DVREEALST-GGLDLIFLPGLGFDKDGNRLGRGKGYYDTYLKRCVQHQEV-------KPY 189

Query: 384 LVALSYSVQIMDEGVIPLTPNDVLVDALV 407
            +AL++  QI  +  IP+  +D+ VD ++
Sbjct: 190 TMALAFKEQICPQ--IPVDEHDMKVDEVL 196

[Close]

BLAST of Spg022456 vs. ExPASy Swiss-Prot
Match: P80405 (5-formyltetrahydrofolate cyclo-ligase OS=Oryctolagus cuniculus OX=9986 GN=MTHFS PE=1 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 1.2e-16
Identity = 60/211 (28.44%), Postives = 112/211 (53.08%), Query Frame = 0


Query: 199 AIFEQKRVLRSSVRKALKAMDPSLRSQEDNAIQSIVLEAPWFKSSQRLCAYVSCSALREV 258
           A+   KR LR+ +++ L+A+    R +    +   V+    ++ SQR+  ++S     E+
Sbjct: 5   AVSGAKRSLRAELKQRLRAISAEERLRCQRLLTQKVIAHRQYQKSQRISIFLSMP--DEI 64

Query: 259 DTSRLLSETLQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIA---NSMNILEPAP 318
           +T  ++ +  Q        K  ++PR   +++HM M  ++  D++ +    S NI +P+ 
Sbjct: 65  ETEEIIKDIFQQ------GKVCFIPRYRLQSNHMDMVKLASADEISSLPKTSWNIHQPS- 124

Query: 319 VDGDGNEREDVMQTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQ 378
              + + RE+ + T   +DL  +PGL FD++G RLGRG GYYDT+L+   +   A+    
Sbjct: 125 ---ESDTREEALAT-GGLDLIFMPGLGFDRNGNRLGRGRGYYDTYLQRCLQQQGAK---- 184

Query: 379 PLLVALSYSVQIMDEGVIPLTPNDVLVDALV 407
           P  +AL++  QI  +  +P+   DV VD ++
Sbjct: 185 PYTIALAFREQICPQ--VPVDDTDVSVDEVL 196

[Close]

BLAST of Spg022456 vs. ExPASy Swiss-Prot
Match: Q9XWE6 (Probable 5-formyltetrahydrofolate cyclo-ligase OS=Caenorhabditis elegans OX=6239 GN=Y106G6E.4 PE=3 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 1.3e-13
Identity = 58/213 (27.23%), Postives = 99/213 (46.48%), Query Frame = 0


Query: 197 LKAIFEQKRVLRSSVRKALKAMDPSLRSQEDNAIQSIVLEAPWFKSSQRLCAYVSCSALR 256
           + AI E K  LR  ++  L  +      ++  A+   ++E+ WF+ S+RL  YVS S   
Sbjct: 1   MSAIKEVKSELRQFMKTLLGKISKEETQRQTEAVFEKIIESKWFQESKRLSVYVSTSG-- 60

Query: 257 EVDTSRLLSETLQHPPKDGYSKKIYVPRVEDKNSHMRMFNI---SRMDDLIANSMNILEP 316
           E+ T  ++ + L+        K++++P+    ++ M M  +   +  D+L +    I +P
Sbjct: 61  EIQTDSIIQKALE------MGKEVFIPQFTKGSTAMDMVRVPDQTAFDNLPSTLWGIRQP 120

Query: 317 APVDGDGNEREDVMQTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNW 376
            P       +        P+DL L PG+AF   G R G G GYYD F   +    K    
Sbjct: 121 EP-----KWKWQSYHETGPLDLILAPGVAFSPYGLRCGHGKGYYDRFFSTHH---KHFPE 180

Query: 377 KQPLLVALSYSVQIMDEGVIPLTPNDVLVDALV 407
             P  + L+   QI+  G IP++  DV +D ++
Sbjct: 181 NSPKKIGLALREQII--GTIPISETDVELDEVI 195

[Close]

BLAST of Spg022456 vs. ExPASy TrEMBL
Match: A0A6J1JM66 (5-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485651 PE=4 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 3.9e-167
Identity = 306/336 (91.07%), Postives = 318/336 (94.64%), Query Frame = 0


Query: 89  MIKSRLLQGNETNSPVSGFQNSTSAFRLTNQRSPAGVHVGRSPKARRWAISEAPMVRNYK 148
           MIKS+LLQGN+TN PV G QNST+A RLTN RSPAGVHVGRSPKARR AISE+PMVRNYK
Sbjct: 1   MIKSQLLQGNKTNLPVHGLQNSTTASRLTNPRSPAGVHVGRSPKARRRAISESPMVRNYK 60

Query: 149 LLPIAANSLFNRLPPSAFQQALPPLSFRSSGSPKMASNESSKPENEDQLKAIFEQKRVLR 208
           LLPIAA+SLFNRLPPSAFQ AL PLS  SSGSPKMA NE  KPENE+QLKA+FEQKRV+R
Sbjct: 61  LLPIAASSLFNRLPPSAFQPALRPLSRCSSGSPKMARNEGIKPENEEQLKALFEQKRVIR 120

Query: 209 SSVRKALKAMDPSLRSQEDNAIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSETL 268
           SSVRKALKAMDPSLRSQEDNAIQ+IVLEAPWFKSSQRLCAYVSCSALREVDTSR+LSETL
Sbjct: 121 SSVRKALKAMDPSLRSQEDNAIQNIVLEAPWFKSSQRLCAYVSCSALREVDTSRILSETL 180

Query: 269 QHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVMQ 328
           QHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVMQ
Sbjct: 181 QHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVMQ 240

Query: 329 TKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQIM 388
           TKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAK RNWKQPLLVALSYSVQIM
Sbjct: 241 TKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQIM 300

Query: 389 DEGVIPLTPNDVLVDALVSPSGVIPTSSAGLDKESI 425
           DEG+IPLTPNDVLVD LVSPSGVIP SSAGLDK  +
Sbjct: 301 DEGIIPLTPNDVLVDVLVSPSGVIPISSAGLDKMKV 336

[Close]

BLAST of Spg022456 vs. ExPASy TrEMBL
Match: A0A6J1JCX7 (5-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485651 PE=4 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 4.8e-165
Identity = 305/338 (90.24%), Postives = 317/338 (93.79%), Query Frame = 0


Query: 89  MIKSRLLQGNETNSPVSGFQNSTSAFRLTNQRSPAGVHVGRSPKARRWAISEAPMVRNYK 148
           MIKS+LLQGN+TN PV G QNST+A RLTN RSPAGVHVGRSPKARR AISE+PMVRNYK
Sbjct: 1   MIKSQLLQGNKTNLPVHGLQNSTTASRLTNPRSPAGVHVGRSPKARRRAISESPMVRNYK 60

Query: 149 LLPIAANSLFNRLPPSAFQQALPPLSFRSSGSPKMASNESSKPENEDQLKAIFEQKRVLR 208
           LLPIAA+SLFNRLPPSAFQ AL PLS  SSGSPKMA NE  KPENE+QLKA+FEQKRV+R
Sbjct: 61  LLPIAASSLFNRLPPSAFQPALRPLSRCSSGSPKMARNEGIKPENEEQLKALFEQKRVIR 120

Query: 209 SSVRKALKAMDPSLRSQEDNAIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSETL 268
           SSVRKALKAMDPSLRSQEDNAIQ+IVLEAPWFKSSQRLCAYVSCSALREVDTSR+LSETL
Sbjct: 121 SSVRKALKAMDPSLRSQEDNAIQNIVLEAPWFKSSQRLCAYVSCSALREVDTSRILSETL 180

Query: 269 QHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNERED--V 328
           QHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNERED   
Sbjct: 181 QHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDGNF 240

Query: 329 MQTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQ 388
           MQTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAK RNWKQPLLVALSYSVQ
Sbjct: 241 MQTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQ 300

Query: 389 IMDEGVIPLTPNDVLVDALVSPSGVIPTSSAGLDKESI 425
           IMDEG+IPLTPNDVLVD LVSPSGVIP SSAGLDK  +
Sbjct: 301 IMDEGIIPLTPNDVLVDVLVSPSGVIPISSAGLDKMKV 338

[Close]

BLAST of Spg022456 vs. ExPASy TrEMBL
Match: A0A0A0LRK4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G007880 PE=4 SV=1)

HSP 1 Score: 589.0 bits (1517), Expect = 1.8e-164
Identity = 301/337 (89.32%), Postives = 316/337 (93.77%), Query Frame = 0


Query: 89  MIKSRLLQGNETNSPVSGFQNSTSAFRLTNQRSPAGVHVGRSPKARRWAISEAPMVRNYK 148
           M+K +LLQGN TN PV+GFQNST+ FRLTNQ SPAGVH+GRSPK ++WAISEAPM+RNYK
Sbjct: 1   MVKYQLLQGNRTNLPVNGFQNSTTGFRLTNQLSPAGVHLGRSPKPQKWAISEAPMLRNYK 60

Query: 149 -LLPIAANSLFNRLPPSAFQQALPPLSFRSSGSPKMASNESSKPENEDQLKAIFEQKRVL 208
            LLPIAANSLFN +  SAFQQ+LPPLS  SSG PKMA+NES KPENEDQLK IF+QKRVL
Sbjct: 61  PLLPIAANSLFNHIQSSAFQQSLPPLSRFSSGYPKMATNESIKPENEDQLKHIFDQKRVL 120

Query: 209 RSSVRKALKAMDPSLRSQEDNAIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSET 268
           RSSVRKALKAMDPS RSQEDN IQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSE 
Sbjct: 121 RSSVRKALKAMDPSFRSQEDNVIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSEI 180

Query: 269 LQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 328
           LQHPPKDG+SKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM
Sbjct: 181 LQHPPKDGHSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 240

Query: 329 QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQI 388
           QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQI
Sbjct: 241 QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQI 300

Query: 389 MDEGVIPLTPNDVLVDALVSPSGVIPTSSAGLDKESI 425
           MDEG+IPLTPNDVLVDALVSPSGVIP SSAGLDK  +
Sbjct: 301 MDEGIIPLTPNDVLVDALVSPSGVIPISSAGLDKMKV 337

[Close]

BLAST of Spg022456 vs. ExPASy TrEMBL
Match: A0A6J1E0S0 (5-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111429770 PE=4 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 9.1e-164
Identity = 300/336 (89.29%), Postives = 315/336 (93.75%), Query Frame = 0


Query: 89  MIKSRLLQGNETNSPVSGFQNSTSAFRLTNQRSPAGVHVGRSPKARRWAISEAPMVRNYK 148
           MIKS+LLQGN+TN PV G QNST+A RLTN R+PAGVHVGRSPKARR A+SE+PMVRNYK
Sbjct: 1   MIKSQLLQGNKTNLPVHGLQNSTTASRLTNPRNPAGVHVGRSPKARRRAVSESPMVRNYK 60

Query: 149 LLPIAANSLFNRLPPSAFQQALPPLSFRSSGSPKMASNESSKPENEDQLKAIFEQKRVLR 208
           LLPIAA+S FNRLPP+AFQ AL PLS  SS SPKMA NE  KPENE+QLKA+FEQKRV+R
Sbjct: 61  LLPIAASSPFNRLPPTAFQPALRPLSRCSSRSPKMARNEGIKPENEEQLKALFEQKRVIR 120

Query: 209 SSVRKALKAMDPSLRSQEDNAIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSETL 268
           SSVRKALKAMDPSLRSQEDNAIQ+IVLEAPWFKSSQRLCAYVSCSALREVDTSR+LSETL
Sbjct: 121 SSVRKALKAMDPSLRSQEDNAIQNIVLEAPWFKSSQRLCAYVSCSALREVDTSRILSETL 180

Query: 269 QHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVMQ 328
           QHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 
Sbjct: 181 QHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVML 240

Query: 329 TKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQIM 388
           TKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAK RNWKQPLLVALSYSVQIM
Sbjct: 241 TKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQIM 300

Query: 389 DEGVIPLTPNDVLVDALVSPSGVIPTSSAGLDKESI 425
           DEG+IPLTPNDVLVD LVSPSGVIP SSAGLDK  +
Sbjct: 301 DEGIIPLTPNDVLVDVLVSPSGVIPISSAGLDKMKV 336

[Close]

BLAST of Spg022456 vs. ExPASy TrEMBL
Match: A0A6J1E1N0 (5-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111429770 PE=4 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 1.1e-161
Identity = 299/338 (88.46%), Postives = 314/338 (92.90%), Query Frame = 0


Query: 89  MIKSRLLQGNETNSPVSGFQNSTSAFRLTNQRSPAGVHVGRSPKARRWAISEAPMVRNYK 148
           MIKS+LLQGN+TN PV G QNST+A RLTN R+PAGVHVGRSPKARR A+SE+PMVRNYK
Sbjct: 1   MIKSQLLQGNKTNLPVHGLQNSTTASRLTNPRNPAGVHVGRSPKARRRAVSESPMVRNYK 60

Query: 149 LLPIAANSLFNRLPPSAFQQALPPLSFRSSGSPKMASNESSKPENEDQLKAIFEQKRVLR 208
           LLPIAA+S FNRLPP+AFQ AL PLS  SS SPKMA NE  KPENE+QLKA+FEQKRV+R
Sbjct: 61  LLPIAASSPFNRLPPTAFQPALRPLSRCSSRSPKMARNEGIKPENEEQLKALFEQKRVIR 120

Query: 209 SSVRKALKAMDPSLRSQEDNAIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSETL 268
           SSVRKALKAMDPSLRSQEDNAIQ+IVLEAPWFKSSQRLCAYVSCSALREVDTSR+LSETL
Sbjct: 121 SSVRKALKAMDPSLRSQEDNAIQNIVLEAPWFKSSQRLCAYVSCSALREVDTSRILSETL 180

Query: 269 QHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNERED--V 328
           QHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNERED   
Sbjct: 181 QHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDGNF 240

Query: 329 MQTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQ 388
           M TKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAK RNWKQPLLVALSYSVQ
Sbjct: 241 MLTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQ 300

Query: 389 IMDEGVIPLTPNDVLVDALVSPSGVIPTSSAGLDKESI 425
           IMDEG+IPLTPNDVLVD LVSPSGVIP SSAGLDK  +
Sbjct: 301 IMDEGIIPLTPNDVLVDVLVSPSGVIPISSAGLDKMKV 338

[Close]

BLAST of Spg022456 vs. TAIR 10
Match: AT5G13050.1 (5-formyltetrahydrofolate cycloligase )

HSP 1 Score: 326.2 bits (835), Expect = 4.3e-89
Identity = 159/239 (66.53%), Postives = 200/239 (83.68%), Query Frame = 0


Query: 175 FRSSGSPKMASNESSKPENEDQLKAIFEQKRVLRSSVRKALKAMDPSLRSQEDNAIQSIV 234
           FR S + +     S+  +N+++L +IF+QKRV+RS+VRK+LKAMDPSLR+Q+D AIQ  V
Sbjct: 31  FRLSPATRPIVAMSTTSKNQEELDSIFKQKRVVRSTVRKSLKAMDPSLRTQQDEAIQKTV 90

Query: 235 LEAPWFKSSQRLCAYVSCSALREVDTSRLLSETLQHPPKDGYSKKIYVPRVEDKNSHMRM 294
           LEAPWFKS + LCAY+SC +L EVDTS++LSE LQHP  +   KK+YVP VEDKNS+MRM
Sbjct: 91  LEAPWFKSCKGLCAYISCKSLNEVDTSKILSEILQHPDSN-TQKKLYVPWVEDKNSNMRM 150

Query: 295 FNISRMDDLIANSMNILEPAPVDGDGNEREDVMQTKDPIDLFLLPGLAFDKSGRRLGRGG 354
            +IS M+DL+ANSMNILEPAPVD  GN+REDV+Q  +PIDLF+LPGLAFD+ GRRLGRGG
Sbjct: 151 LHISHMEDLVANSMNILEPAPVDAQGNDREDVLQADEPIDLFILPGLAFDRCGRRLGRGG 210

Query: 355 GYYDTFLKNYQELAKARNWKQPLLVALSYSVQIMDEGVIPLTPNDVLVDALVSPSGVIP 414
           GYYDTFLK YQ+ AK + W+ PL+VALSYS QI+++G IP+TPNDVL+DALV+PSGV+P
Sbjct: 211 GYYDTFLKRYQDRAKEKGWRYPLMVALSYSPQILEDGSIPVTPNDVLIDALVTPSGVVP 268

[Close]

BLAST of Spg022456 vs. TAIR 10
Match: AT5G13050.2 (5-formyltetrahydrofolate cycloligase )

HSP 1 Score: 234.6 bits (597), Expect = 1.7e-61
Identity = 117/179 (65.36%), Postives = 147/179 (82.12%), Query Frame = 0


Query: 175 FRSSGSPKMASNESSKPENEDQLKAIFEQKRVLRSSVRKALKAMDPSLRSQEDNAIQSIV 234
           FR S + +     S+  +N+++L +IF+QKRV+RS+VRK+LKAMDPSLR+Q+D AIQ  V
Sbjct: 31  FRLSPATRPIVAMSTTSKNQEELDSIFKQKRVVRSTVRKSLKAMDPSLRTQQDEAIQKTV 90

Query: 235 LEAPWFKSSQRLCAYVSCSALREVDTSRLLSETLQHPPKDGYSKKIYVPRVEDKNSHMRM 294
           LEAPWFKS + LCAY+SC +L EVDTS++LSE LQHP  +   KK+YVP VEDKNS+MRM
Sbjct: 91  LEAPWFKSCKGLCAYISCKSLNEVDTSKILSEILQHPDSN-TQKKLYVPWVEDKNSNMRM 150

Query: 295 FNISRMDDLIANSMNILEPAPVDGDGNEREDVMQTKDPIDLFLLPGLAFDKSGRRLGRG 354
            +IS M+DL+ANSMNILEPAPVD  GN+REDV+Q  +PIDLF+LPGLAFD+ GRRLGRG
Sbjct: 151 LHISHMEDLVANSMNILEPAPVDAQGNDREDVLQADEPIDLFILPGLAFDRCGRRLGRG 208

The following BLAST results are available for this feature:

BLAST of Spg022456 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022988398.1	8.1e-167	91.07	5-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X2 [Cucurbita ...	[more]
XP_022988397.1	1.0e-164	90.24	5-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X1 [Cucurbita ...	[more]
KGN63636.2	1.7e-164	89.35	hypothetical protein Csa_013278 [Cucumis sativus]	[more]
XP_004138292.1	3.8e-164	89.32	5-formyltetrahydrofolate cyclo-ligase, mitochondrial [Cucumis sativus]	[more]
XP_022921537.1	1.9e-163	89.29	5-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X2 [Cucurbita ...	[more]

BLAST of Spg022456 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L539	6.0e-88	66.53	5-formyltetrahydrofolate cyclo-ligase, mitochondrial OS=Arabidopsis thaliana OX=...	[more]
P49914	3.8e-18	30.37	5-formyltetrahydrofolate cyclo-ligase OS=Homo sapiens OX=9606 GN=MTHFS PE=1 SV=2	[more]
Q9D110	4.2e-17	30.62	5-formyltetrahydrofolate cyclo-ligase OS=Mus musculus OX=10090 GN=Mthfs PE=2 SV=...	[more]
P80405	1.2e-16	28.44	5-formyltetrahydrofolate cyclo-ligase OS=Oryctolagus cuniculus OX=9986 GN=MTHFS ...	[more]
Q9XWE6	1.3e-13	27.23	Probable 5-formyltetrahydrofolate cyclo-ligase OS=Caenorhabditis elegans OX=6239...	[more]

BLAST of Spg022456 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JM66	3.9e-167	91.07	5-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X2 OS=Cucurbit...	[more]
A0A6J1JCX7	4.8e-165	90.24	5-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X1 OS=Cucurbit...	[more]
A0A0A0LRK4	1.8e-164	89.32	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G007880 PE=4 SV=1	[more]
A0A6J1E0S0	9.1e-164	89.29	5-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X2 OS=Cucurbit...	[more]
A0A6J1E1N0	1.1e-161	88.46	5-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X1 OS=Cucurbit...	[more]

BLAST of Spg022456 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G13050.1	4.3e-89	66.53	5-formyltetrahydrofolate cycloligase	[more]
AT5G13050.2	1.7e-61	65.36	5-formyltetrahydrofolate cycloligase	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002698	5-formyltetrahydrofolate cyclo-ligase	TIGRFAM	TIGR02727	TIGR02727	coord: 204..407 e-value: 1.7E-29 score: 101.0
IPR002698	5-formyltetrahydrofolate cyclo-ligase	PFAM	PF01812	5-FTHF_cyc-lig	coord: 204..408 e-value: 2.2E-32 score: 112.4
IPR024185	5-formyltetrahydrofolate cyclo-ligase-like domain superfamily	GENE3D	3.40.50.10420		coord: 196..411 e-value: 8.9E-54 score: 184.1
None	No IPR available	PIRSR	PIRSR006806-1	PIRSR006806-1	coord: 203..317 e-value: 1.1E-10 score: 39.5 coord: 331..407 e-value: 7.4E-17 score: 59.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 176..195
None	No IPR available	PANTHER	PTHR23407	ATPASE INHIBITOR/5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE	coord: 172..421
None	No IPR available	PANTHER	PTHR23407:SF10	5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE, MITOCHONDRIAL-LIKE ISOFORM X1	coord: 172..421
IPR037171	NagB/RpiA transferase-like	SUPERFAMILY	100950	NagB/RpiA/CoA transferase-like	coord: 203..414

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg022456.1	Spg022456.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0009396	folic acid-containing compound biosynthetic process
biological_process	GO:0006355	regulation of transcription, DNA-templated
biological_process	GO:0035999	tetrahydrofolate interconversion
cellular_component	GO:0005739	mitochondrion
cellular_component	GO:0031213	RSF complex
molecular_function	GO:0030272	5-formyltetrahydrofolate cyclo-ligase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0046872	metal ion binding