Spg022435 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg022435
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Pectinesterase
Location	scaffold2: 7954959 .. 7958191 (-)
RNA-Seq Expression	Spg022435
Synteny	Spg022435

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGTCACTCGAAAAGTTAGGTAAATATTTAAAGAAGTTGAGTTAACAAGAAGCAAAAAATTCCCAAGAAAACAAAATGGGTATGAATTTACAAAACAGCCCACCACCAACACCAACACCCAAGGCTGTTGAATGAGAAACAAATTATTAAGAAAGAATCACAGACAGAAAGAAAGAGGAAAACATTCACGGGCAACTCCAAACAAACAACAGGAAGTGTTCAATTTCTTTGATACTCTAAACTCAACCCACCATTTTTATATCTCAAAAGTGCAACCTGTCTTCTGTTTGTGGCTTATATTCAATCTTTCTCTCTCTTCTCGTCTGTCAGTGGAACCGCCCGTGAACTCTAACTCATTCCTTTTCATCGATTCTCTAATTTCCCTTCGCCTTTCCACCTAATGCACTTCTCCCATTAACCTCTATATAAACCCATTTTCGAATTCCTCTGTTTTCAGCTTCAGATTCTTCCTCGAACCATGTCAAAACTTCAGCCTCCTTTCACTCTCTCTCTTCTTCTTCTTCTTCTGCTGCTCACTCAAACGCACTGCCACACCAAGGGGCTCAAACCCAGAAAATCGGCCAGGAGTGCTCTTCCGAAGAATTCGACCAAAACCCAATTCTCAGAACAGCAATTTATGAAGTGGGTCAAATTCGTCGGCAGCCTCAAACACTCTGTTTTCAGAACGGCGAAGAACAAGCTTTTTCCTTCCTACACGCTCCACGTGGCGAAAAATCCGGCCGCCGGCGACTTCACGTCGATTCAAGACGCCGTCGATTCCCTCCCCTTCATCAATTTGGTCAGAGTTGTAATCAAGGTTCATGCAGGTGTTTACACGTGAGTACGAAATCCTACTAAGAATGCTCTGTTTTCTTCTCCTCTGTTTTTTTTGTTTGGTTTCTTCTCGTGTCCTTTTCTAATCATTGTCGAGAATCGACATTATTTCCGAATGCCACTCTAATTTCAACAGAGAAAAAGTGAACATACCACCGTTGAAATCGTTCATAACAATCGAAGGAGCAGGCGCAGACAAAACGATCGTTCAATGGGGGGACACAGCACAAACGCCAGGCCCAAAGGGGCAGCCAATGGGGACCTACAACTCAGCAACATTCGCAGTAAATTCCCCTTATTTCATAGCCAAGAACATCACATTCAAGGTAAATCAAACCCCTCTCCAAACCCCAATTCATTTCCTTAAAAACTGAAACGCGTGCGTACGTGTACGTGTGCGTGTGCGTGCAGAACACGACTCCTGTGCCAGCGCCAGGAGCGATAGGGAAGCAGGCAGTGGCGTTCAGAATCTCGGCCGACACGGCGGCATTCTTCGGGTGCAGATTCCTGGGGGCACAGGACACATTGTACGACCATCTGGGTCGGCATTACTACAAGGATTGTTACATAGAAGGGTCAGTGGACTTCATATTTGGAAACGGGCTGTCTCTGTTCGAGGTGATTGTGATTATGAATGGAAAATTAGATTTTGATTTGAATGTTGAAACGTAATTGTAATGGAAGGAATGGTTGAATTTGGAAACAGGGGTGTCACGTGCACGCAATAGCGCAGTATACAGGGGCGTTGACAGCGCAGGGGAGGAGCAGCCTGCTGGAGGACACAGGGTTTTCGTTCGTAAACTGTAAGGTCACGGGGTCTGGAGCCCTATACTTGGGGAGGGCATGGGGGCCCTTCTCTAGGGTCGTCTTCGCTTATACTTACATGGACAATATCATCATTCCCAAAGGCTGGTATAATTGGGGCGATCCCAACCGAGAAATGTACCTTTCTCTCTCTCTCTCTCTCTCTCTCTTTCTCTTAAGCTTTTGACTTTGTTCATTCAAATGCTATGTTTTCTCCAATTTCGTTAAATTATAAGTTTAGTCATGAATTCGAGCGCCCGAGCGAAGGTTTAAGAAAATTGTGTTTGAGACACTAGTTTAGGAAGGTGAATTTAAGAAACGGAGTTCGAATAGTGAGTTATTGTTTAAGTTTGAAGCCAAATTTACCCTTTGAGTTAGTTCATTTAGTTAAATTATAAGTTTGGTCATGAATTGGTTCTTGAATTTAGGTAGAGCATTCGATTCTAAGAGAATTGTGTTTGGAATGTAAGTTTAGGAAGCGGGATTTAAAAAAATTGTGTGTGGGGTTCGAATAAATAGCGAGTTACTGTTCATGTCTATTGGTTTCTCCCACCAAACTTACTTTTTACAACTGAATCTTCTTACACATCACCAATATGCTGATGATTATTTTTTTTTTCTCATTTTCTACCTTTCTCTTAATTGTCAAGATTTTAATGGATCTCTAACTGATAAAGTAACTAGATAGATCCAACTTTGAACTTTTAGATTACTTGAAATGAATAGATACTAGTTTGAAAGTTGAACTACAAAACTTGGTTGAAAAATCACATAAAATACCCATATGAAGAGAGAACATAATTCCAAAACAGAGCAGAAGAAGAAAGCCACAAAATAAGCAGAGAGGTTAGTTTGCAGAGCCAACGCATGGAAGAAACAAGAACCTGTCCCACGCTGCTAAGTCTACGTGGAATCCACCAACCAAACAAAACACAATTAAAAAAACGACAAACATTTTCAAGTAGCTGAGAGACCACCGCCCCACCAGCGCCGCCGCCGCCGCCACCTAGCCTCTGAGAGTGGTGGTTCTGGGGCGGTGGCATTTTCCCCAATTGGCCATCTTTGATCTGTCACAGAGCATGCGCATGCAGGGGCATTTTGGTAATTCTCTTAATGTTTTTTAATTTTTATTTTGTAGGACTGTGTTTTATGGGCAGTACAAATGTACAGGAGAAGGAGCTAGCTTTGCAGGCAGAGTTTCATGGTCCAGAGAGCTAACTGATGAGGAAGCCAAGCCTTTTATTTCCCTTAGCTTCATTGATGGCTCTGAATGGATCAAAATTTAAATTATTATTCACTTTTTTTTTTCTTTTTTCTTATACTACTCATTACTCAATAATTTATCAGAAATTAAAATACCATATGGGTTTAGCAGCTAGCTAGGCTACACTCTGTTGTATTTAATCATTTTTCACTTTATTCATAAATCTATAAAATTGTATTCACAAAATTACATATGCATTTATTTTGTCCTTTCATTATGTATTTATGTTAGTATTGTGACATTTTTGTCCCTGTCCGGAATTTATTTATTCACCTCGCTATAATATATATGTGGGCCAGAAATTTGATTAAAAATATATATATATATGTGGG

mRNA sequence

ATGTCAAAACTTCAGCCTCCTTTCACTCTCTCTCTTCTTCTTCTTCTTCTGCTGCTCACTCAAACGCACTGCCACACCAAGGGGCTCAAACCCAGAAAATCGGCCAGGAGTGCTCTTCCGAAGAATTCGACCAAAACCCAATTCTCAGAACAGCAATTTATGAAGTGGGTCAAATTCGTCGGCAGCCTCAAACACTCTGTTTTCAGAACGGCGAAGAACAAGCTTTTTCCTTCCTACACGCTCCACGTGGCGAAAAATCCGGCCGCCGGCGACTTCACGTCGATTCAAGACGCCGTCGATTCCCTCCCCTTCATCAATTTGGTCAGAGTTGTAATCAAGGTTCATGCAGGTGTTTACACAGAAAAAGTGAACATACCACCGTTGAAATCGTTCATAACAATCGAAGGAGCAGGCGCAGACAAAACGATCGTTCAATGGGGGGACACAGCACAAACGCCAGGCCCAAAGGGGCAGCCAATGGGGACCTACAACTCAGCAACATTCGCAGTAAATTCCCCTTATTTCATAGCCAAGAACATCACATTCAAGAACACGACTCCTGTGCCAGCGCCAGGAGCGATAGGGAAGCAGGCAGTGGCGTTCAGAATCTCGGCCGACACGGCGGCATTCTTCGGGTGCAGATTCCTGGGGGCACAGGACACATTGTACGACCATCTGGGTCGGCATTACTACAAGGATTGTTACATAGAAGGGTCAGTGGACTTCATATTTGGAAACGGGCTGTCTCTGTTCGAGGGGTGTCACGTGCACGCAATAGCGCAGTATACAGGGGCGTTGACAGCGCAGGGGAGGAGCAGCCTGCTGGAGGACACAGGGTTTTCGTTCGTAAACTGTAGAGCATTCGATTCTAAGAGAATTGTGTTTGGAATGACTGTGTTTTATGGGCAGTACAAATGTACAGGAGAAGGAGCTAGCTTTGCAGGCAGAGTTTCATGGTCCAGAGAGCTAACTGATGAGGAAGCCAAGCCTTTTATTTCCCTTAGCTTCATTGATGGCTCTGAATGGATCAAAATTTAA

Coding sequence (CDS)

ATGTCAAAACTTCAGCCTCCTTTCACTCTCTCTCTTCTTCTTCTTCTTCTGCTGCTCACTCAAACGCACTGCCACACCAAGGGGCTCAAACCCAGAAAATCGGCCAGGAGTGCTCTTCCGAAGAATTCGACCAAAACCCAATTCTCAGAACAGCAATTTATGAAGTGGGTCAAATTCGTCGGCAGCCTCAAACACTCTGTTTTCAGAACGGCGAAGAACAAGCTTTTTCCTTCCTACACGCTCCACGTGGCGAAAAATCCGGCCGCCGGCGACTTCACGTCGATTCAAGACGCCGTCGATTCCCTCCCCTTCATCAATTTGGTCAGAGTTGTAATCAAGGTTCATGCAGGTGTTTACACAGAAAAAGTGAACATACCACCGTTGAAATCGTTCATAACAATCGAAGGAGCAGGCGCAGACAAAACGATCGTTCAATGGGGGGACACAGCACAAACGCCAGGCCCAAAGGGGCAGCCAATGGGGACCTACAACTCAGCAACATTCGCAGTAAATTCCCCTTATTTCATAGCCAAGAACATCACATTCAAGAACACGACTCCTGTGCCAGCGCCAGGAGCGATAGGGAAGCAGGCAGTGGCGTTCAGAATCTCGGCCGACACGGCGGCATTCTTCGGGTGCAGATTCCTGGGGGCACAGGACACATTGTACGACCATCTGGGTCGGCATTACTACAAGGATTGTTACATAGAAGGGTCAGTGGACTTCATATTTGGAAACGGGCTGTCTCTGTTCGAGGGGTGTCACGTGCACGCAATAGCGCAGTATACAGGGGCGTTGACAGCGCAGGGGAGGAGCAGCCTGCTGGAGGACACAGGGTTTTCGTTCGTAAACTGTAGAGCATTCGATTCTAAGAGAATTGTGTTTGGAATGACTGTGTTTTATGGGCAGTACAAATGTACAGGAGAAGGAGCTAGCTTTGCAGGCAGAGTTTCATGGTCCAGAGAGCTAACTGATGAGGAAGCCAAGCCTTTTATTTCCCTTAGCTTCATTGATGGCTCTGAATGGATCAAAATTTAA

Protein sequence

MSKLQPPFTLSLLLLLLLLTQTHCHTKGLKPRKSARSALPKNSTKTQFSEQQFMKWVKFVGSLKHSVFRTAKNKLFPSYTLHVAKNPAAGDFTSIQDAVDSLPFINLVRVVIKVHAGVYTEKVNIPPLKSFITIEGAGADKTIVQWGDTAQTPGPKGQPMGTYNSATFAVNSPYFIAKNITFKNTTPVPAPGAIGKQAVAFRISADTAAFFGCRFLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNGLSLFEGCHVHAIAQYTGALTAQGRSSLLEDTGFSFVNCRAFDSKRIVFGMTVFYGQYKCTGEGASFAGRVSWSRELTDEEAKPFISLSFIDGSEWIKI

Homology

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BLAST of Spg022435 vs. NCBI nr
Match: XP_022145102.1 (probable pectinesterase 53 [Momordica charantia])

HSP 1 Score: 627.9 bits (1618), Expect = 5.3e-176
Identity = 318/379 (83.91%), Postives = 329/379 (86.81%), Query Frame = 0


Query: 1   MSKLQPPFTLSLLLLLLLLTQTHCHTKGLKPRKSARSALPKNSTKTQFSEQQFMKWVKFV 60
           MS+LQP   L LL L L +TQ+HCHTKG++PR   RSALPKNST TQFSEQQFMKWVKFV
Sbjct: 1   MSRLQPLLLLLLLSLSLSVTQSHCHTKGIRPRNEVRSALPKNSTNTQFSEQQFMKWVKFV 60

Query: 61  GSLKHSVFRTAKNKLFPSYTLHVAKNPAAGDFTSIQDAVDSLPFINLVRVVIKVHAGVYT 120
           G+LKHSVFRTAKNK+FPSYTLHVAKNPAAGDFTSIQDAVDSLPFINLVRVVIKVHAGVYT
Sbjct: 61  GTLKHSVFRTAKNKIFPSYTLHVAKNPAAGDFTSIQDAVDSLPFINLVRVVIKVHAGVYT 120

Query: 121 EKVNIPPLKSFITIEGAGADKTIVQWGDTAQTPGPKGQPMGTYNSATFAVNSPYFIAKNI 180
           EKVNIPPLKSFITIEGAGADKTIVQWGDTAQTPGPKGQPMGTYNSATFAVNSPYFIAKNI
Sbjct: 121 EKVNIPPLKSFITIEGAGADKTIVQWGDTAQTPGPKGQPMGTYNSATFAVNSPYFIAKNI 180

Query: 181 TFKNTTPVPAPGAIGKQAVAFRISADTAAFFGCRFLGAQDTLYDHLGRHYYKDCYIEGSV 240
           TFKNTTPVPAPGAIGKQAVAFRISADTAAFFGC+FLGAQDTLYDHLGRHYYKDCYIEGSV
Sbjct: 181 TFKNTTPVPAPGAIGKQAVAFRISADTAAFFGCKFLGAQDTLYDHLGRHYYKDCYIEGSV 240

Query: 241 DFIFGNGLSLFEGCHVHAIAQYTGALTAQGRSSLLEDTGFSFVNCRAFDS---------- 300
           DFIFGNGLSLFEGCHVHAIAQYTGALTAQGRSSLLEDTGFSFVNC+   S          
Sbjct: 241 DFIFGNGLSLFEGCHVHAIAQYTGALTAQGRSSLLEDTGFSFVNCKVTGSGALYLGRAWG 300

Query: 301 --KRIVFG----------------------MTVFYGQYKCTGEGASFAGRVSWSRELTDE 346
              R+VF                       MTVFYGQYKCTGEGAS+AGRVSWSRELTDE
Sbjct: 301 PFSRVVFAYTYMDNIIIPKGWYNWGDPNREMTVFYGQYKCTGEGASYAGRVSWSRELTDE 360

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BLAST of Spg022435 vs. NCBI nr
Match: KAG6588003.1 (putative pectinesterase 53, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 623.6 bits (1607), Expect = 1.0e-174
Identity = 317/382 (82.98%), Postives = 328/382 (85.86%), Query Frame = 0


Query: 1   MSKLQPPFTLS---LLLLLLLLTQTHCHTKGLKPRKSARSALPKNSTKTQFSEQQFMKWV 60
           MSKLQPPF  S   LLLLLLLLTQTHCHTKG++P+KS R+ LPKNSTKTQFSEQQFMKWV
Sbjct: 1   MSKLQPPFAFSLLLLLLLLLLLTQTHCHTKGIRPKKSPRTILPKNSTKTQFSEQQFMKWV 60

Query: 61  KFVGSLKHSVFRTAKNKLFPSYTLHVAKNPAAGDFTSIQDAVDSLPFINLVRVVIKVHAG 120
           KFVGSLKHSVFRTAKNKLFPSYTLHVAKNPAAGDFTSIQDA+DSLPFINL+RVVIKVHAG
Sbjct: 61  KFVGSLKHSVFRTAKNKLFPSYTLHVAKNPAAGDFTSIQDAIDSLPFINLIRVVIKVHAG 120

Query: 121 VYTEKVNIPPLKSFITIEGAGADKTIVQWGDTAQTPGPKGQPMGTYNSATFAVNSPYFIA 180
           VY EKV IPP KSFITIEGAGA KTIVQWGDTAQTPGPKGQP+GTYNSATFAVNSPYFIA
Sbjct: 121 VYKEKVIIPPFKSFITIEGAGAAKTIVQWGDTAQTPGPKGQPLGTYNSATFAVNSPYFIA 180

Query: 181 KNITFKNTTPVPAPGAIGKQAVAFRISADTAAFFGCRFLGAQDTLYDHLGRHYYKDCYIE 240
           KNITFKNTTPVP PGAIGKQAVAFRIS DTAAF+GCRFLGAQDTLYDHLGRHYYKDCYIE
Sbjct: 181 KNITFKNTTPVPPPGAIGKQAVAFRISGDTAAFYGCRFLGAQDTLYDHLGRHYYKDCYIE 240

Query: 241 GSVDFIFGNGLSLFEGCHVHAIAQYTGALTAQGRSSLLEDTGFSFVNCRAFDS------- 300
           GSVDFIFGNGLSLFEGCHVHAIA+YTGALTAQGRSSLLEDTGFSFVNC+   S       
Sbjct: 241 GSVDFIFGNGLSLFEGCHVHAIARYTGALTAQGRSSLLEDTGFSFVNCKVTGSGALYLGR 300

Query: 301 -----KRIVFG----------------------MTVFYGQYKCTGEGASFAGRVSWSREL 346
                 R+VF                       MTVFYGQYKCTGEG  FAGRVSWSREL
Sbjct: 301 AWGPFSRVVFAYTYMDNIIIPKGWYNWGDPNREMTVFYGQYKCTGEGGRFAGRVSWSREL 360

[Close]

BLAST of Spg022435 vs. NCBI nr
Match: KAG7021897.1 (putative pectinesterase 53, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 623.2 bits (1606), Expect = 1.3e-174
Identity = 317/383 (82.77%), Postives = 328/383 (85.64%), Query Frame = 0


Query: 1   MSKLQPPFTLS----LLLLLLLLTQTHCHTKGLKPRKSARSALPKNSTKTQFSEQQFMKW 60
           MSKLQPPF  S    LLLLLLLLTQTHCHTKG++P+KS R+ LPKNSTKTQFSEQQFMKW
Sbjct: 1   MSKLQPPFAFSLLLLLLLLLLLLTQTHCHTKGIRPKKSPRTILPKNSTKTQFSEQQFMKW 60

Query: 61  VKFVGSLKHSVFRTAKNKLFPSYTLHVAKNPAAGDFTSIQDAVDSLPFINLVRVVIKVHA 120
           VKFVGSLKHSVFRTAKNKLFPSYTLHVAKNPAAGDFTSIQDA+DSLPFINL+RVVIKVHA
Sbjct: 61  VKFVGSLKHSVFRTAKNKLFPSYTLHVAKNPAAGDFTSIQDAIDSLPFINLIRVVIKVHA 120

Query: 121 GVYTEKVNIPPLKSFITIEGAGADKTIVQWGDTAQTPGPKGQPMGTYNSATFAVNSPYFI 180
           GVY EKV IPP KSFITIEGAGA KTIVQWGDTAQTPGPKGQP+GTYNSATFAVNSPYFI
Sbjct: 121 GVYKEKVIIPPFKSFITIEGAGAAKTIVQWGDTAQTPGPKGQPLGTYNSATFAVNSPYFI 180

Query: 181 AKNITFKNTTPVPAPGAIGKQAVAFRISADTAAFFGCRFLGAQDTLYDHLGRHYYKDCYI 240
           AKNITFKNTTPVP PGAIGKQAVAFRIS DTAAF+GCRFLGAQDTLYDHLGRHYYKDCYI
Sbjct: 181 AKNITFKNTTPVPPPGAIGKQAVAFRISGDTAAFYGCRFLGAQDTLYDHLGRHYYKDCYI 240

Query: 241 EGSVDFIFGNGLSLFEGCHVHAIAQYTGALTAQGRSSLLEDTGFSFVNCRAFDS------ 300
           EGSVDFIFGNGLSLFEGCHVHAIA+YTGALTAQGRSSLLEDTGFSFVNC+   S      
Sbjct: 241 EGSVDFIFGNGLSLFEGCHVHAIARYTGALTAQGRSSLLEDTGFSFVNCKVTGSGALYLG 300

Query: 301 ------KRIVFG----------------------MTVFYGQYKCTGEGASFAGRVSWSRE 346
                  R+VF                       MTVFYGQYKCTGEG  FAGRVSWSRE
Sbjct: 301 RAWGPFSRVVFAYTYMDNIIIPKGWYNWGDPNREMTVFYGQYKCTGEGGRFAGRVSWSRE 360

[Close]

BLAST of Spg022435 vs. NCBI nr
Match: XP_023529306.1 (probable pectinesterase 53 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 622.5 bits (1604), Expect = 2.2e-174
Identity = 317/383 (82.77%), Postives = 328/383 (85.64%), Query Frame = 0


Query: 1   MSKLQPPFTLS----LLLLLLLLTQTHCHTKGLKPRKSARSALPKNSTKTQFSEQQFMKW 60
           MSKLQPPF  S    LLLLLLLLTQTHCHTKG++P+KS R+ LPKNSTKTQFSEQQFMKW
Sbjct: 1   MSKLQPPFGFSLLLLLLLLLLLLTQTHCHTKGIRPKKSPRTILPKNSTKTQFSEQQFMKW 60

Query: 61  VKFVGSLKHSVFRTAKNKLFPSYTLHVAKNPAAGDFTSIQDAVDSLPFINLVRVVIKVHA 120
           VKFVGSLKHSVFRTAKNKLFPSYTLHVAKNPAAGDFTSIQDA+DSLPFINL+RVVIKVHA
Sbjct: 61  VKFVGSLKHSVFRTAKNKLFPSYTLHVAKNPAAGDFTSIQDAIDSLPFINLIRVVIKVHA 120

Query: 121 GVYTEKVNIPPLKSFITIEGAGADKTIVQWGDTAQTPGPKGQPMGTYNSATFAVNSPYFI 180
           GVY EKV IPP KSFITIEGAGA KTIVQWGDTAQTPGPKGQP+GTYNSATFAVNSPYFI
Sbjct: 121 GVYKEKVIIPPFKSFITIEGAGAAKTIVQWGDTAQTPGPKGQPLGTYNSATFAVNSPYFI 180

Query: 181 AKNITFKNTTPVPAPGAIGKQAVAFRISADTAAFFGCRFLGAQDTLYDHLGRHYYKDCYI 240
           AKNITFKNTTPVP PGAIGKQAVAFRIS DTAAF+GCRFLGAQDTLYDHLGRHYYKDCYI
Sbjct: 181 AKNITFKNTTPVPPPGAIGKQAVAFRISGDTAAFYGCRFLGAQDTLYDHLGRHYYKDCYI 240

Query: 241 EGSVDFIFGNGLSLFEGCHVHAIAQYTGALTAQGRSSLLEDTGFSFVNCRAFDS------ 300
           EGSVDFIFGNGLSLFEGCHVHAIA+YTGALTAQGRSSLLEDTGFSFVNC+   S      
Sbjct: 241 EGSVDFIFGNGLSLFEGCHVHAIARYTGALTAQGRSSLLEDTGFSFVNCKVTGSGALYLG 300

Query: 301 ------KRIVFG----------------------MTVFYGQYKCTGEGASFAGRVSWSRE 346
                  R+VF                       MTVFYGQYKCTGEG  FAGRVSWSRE
Sbjct: 301 RAWGPFSRVVFAYTYMDNIIIPKGWYNWGDPNREMTVFYGQYKCTGEGGRFAGRVSWSRE 360

[Close]

BLAST of Spg022435 vs. NCBI nr
Match: XP_022925076.1 (probable pectinesterase 53 [Cucurbita moschata])

HSP 1 Score: 620.2 bits (1598), Expect = 1.1e-173
Identity = 316/382 (82.72%), Postives = 327/382 (85.60%), Query Frame = 0


Query: 1   MSKLQPPFTLS---LLLLLLLLTQTHCHTKGLKPRKSARSALPKNSTKTQFSEQQFMKWV 60
           MSKLQPPF  S   LLLLLLLLTQTHCHTKG++P+KS R+ LPKNSTKTQFSEQQFMKWV
Sbjct: 1   MSKLQPPFAFSLLLLLLLLLLLTQTHCHTKGIRPKKSPRTILPKNSTKTQFSEQQFMKWV 60

Query: 61  KFVGSLKHSVFRTAKNKLFPSYTLHVAKNPAAGDFTSIQDAVDSLPFINLVRVVIKVHAG 120
           KFVGSLKHSVFRTAKNKLFPSYTLHVAKNPAAGDFTSIQDA+DSLPFINL+RVVIKVHAG
Sbjct: 61  KFVGSLKHSVFRTAKNKLFPSYTLHVAKNPAAGDFTSIQDAIDSLPFINLIRVVIKVHAG 120

Query: 121 VYTEKVNIPPLKSFITIEGAGADKTIVQWGDTAQTPGPKGQPMGTYNSATFAVNSPYFIA 180
           VY EKV IPP KSFITIEGAGA KTIVQWGDTAQT GPKGQP+GTYNSATFAVNSPYFIA
Sbjct: 121 VYKEKVIIPPFKSFITIEGAGAAKTIVQWGDTAQTRGPKGQPLGTYNSATFAVNSPYFIA 180

Query: 181 KNITFKNTTPVPAPGAIGKQAVAFRISADTAAFFGCRFLGAQDTLYDHLGRHYYKDCYIE 240
           KNITFKNTTPVP PGAIGKQAVAFRIS DTAAF+GCRFLGAQDTLYDHLGRHYYKDCYIE
Sbjct: 181 KNITFKNTTPVPPPGAIGKQAVAFRISGDTAAFYGCRFLGAQDTLYDHLGRHYYKDCYIE 240

Query: 241 GSVDFIFGNGLSLFEGCHVHAIAQYTGALTAQGRSSLLEDTGFSFVNCRAFDS------- 300
           GSVDFIFGNGLSLFEGCHVHAIA+YTGALTAQGRSSLLEDTGFSFVNC+   S       
Sbjct: 241 GSVDFIFGNGLSLFEGCHVHAIARYTGALTAQGRSSLLEDTGFSFVNCKVTGSGALYLGR 300

Query: 301 -----KRIVFG----------------------MTVFYGQYKCTGEGASFAGRVSWSREL 346
                 R+VF                       MTVFYGQYKCTGEG  FAGRVSWSREL
Sbjct: 301 AWGPFSRVVFAYTYMDNIIIPKGWYNWGDPNREMTVFYGQYKCTGEGGRFAGRVSWSREL 360

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BLAST of Spg022435 vs. ExPASy Swiss-Prot
Match: Q8VYZ3 (Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 3.6e-143
Identity = 255/374 (68.18%), Postives = 288/374 (77.01%), Query Frame = 0


Query: 6   PPFTLSLLLLLLLLTQTHCHTKGLKPRKSARSALPKNSTKTQFSEQQFMKWVKFVGSLKH 65
           P   L LL++LL  TQT CHTKGL+ R   +  +   S +TQ  E +FMKWV+FVGSLKH
Sbjct: 10  PNLFLLLLVILLCSTQTQCHTKGLRLRPRNQKNMNTTSDRTQNPEDEFMKWVRFVGSLKH 69

Query: 66  SVFRTAKNKLFPSYTLHVAKNPAAGDFTSIQDAVDSLPFINLVRVVIKVHAGVYTEKVNI 125
           SVF+ AKNKLFPSYTL V K    GDFT IQDA+DSLP IN VRVVIKVHAGVY EKV+I
Sbjct: 70  SVFKAAKNKLFPSYTLTVHKKSNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSI 129

Query: 126 PPLKSFITIEGAGADKTIVQWGDTAQTPGPKGQPMGTYNSATFAVNSPYFIAKNITFKNT 185
           PPLK+FITIEG GA+KT V+WGDTAQTP  KG PMGTYNSA+FAVNSP+F+AKNITF+NT
Sbjct: 130 PPLKAFITIEGEGAEKTTVEWGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNT 189

Query: 186 TPVPAPGAIGKQAVAFRISADTAAFFGCRFLGAQDTLYDHLGRHYYKDCYIEGSVDFIFG 245
           TPVP PGA+GKQAVA R+SAD AAFFGCR LGAQDTLYDHLGRHYYKDCYIEGSVDFIFG
Sbjct: 190 TPVPLPGAVGKQAVALRVSADNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFG 249

Query: 246 NGLSLFEGCHVHAIAQYTGALTAQGRSSLLEDTGFSFVNCRAFDS------------KRI 305
           N LSL+EGCHVHAIA   GA+TAQGRSS+LEDTGFSFV C+   +             R+
Sbjct: 250 NALSLYEGCHVHAIADKLGAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRV 309

Query: 306 VFG----------------------MTVFYGQYKCTGEGASFAGRVSWSRELTDEEAKPF 346
           VF                       MTVFYGQYKCTG GA++ GRV+W+RELTDEEAKPF
Sbjct: 310 VFAYTYMDNIILPRGWYNWGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPF 369

[Close]

BLAST of Spg022435 vs. ExPASy Swiss-Prot
Match: Q8LPF3 (Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 1.1e-64
Identity = 150/378 (39.68%), Postives = 192/378 (50.79%), Query Frame = 0


Query: 1   MSKLQPPFTLSLLLLLLLLTQTHCHTKGLKPRKSARSALPKNSTKTQFSEQQFMKWVKFV 60
           M++L         + LLL    HC    L  R S  +A   ++   Q       KW   V
Sbjct: 1   MAQLTNSLNYLFSVSLLLFVSFHC----LCFRFSLVAACSNSTDDQQIQHHHHRKW---V 60

Query: 61  GSLKHSVFRTAKNKLFPSYTLHVAKNPAAGDFTSIQDAVDSLPFINLVRVVIKVHAGVYT 120
           G   H V   + N                  F S+QDAVDS+P  N   + IK+  G Y 
Sbjct: 61  GPSGHKVITVSLN--------------GHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYR 120

Query: 121 EKVNIPPLKSFITIEGAGADKTIVQWGDTAQTPGPKGQPMGTYNSATFAVNSPYFIAKNI 180
           EKV +P  K +IT +GAG D T ++W D A   G  GQ + TY +A+  V + YF A+NI
Sbjct: 121 EKVVVPATKPYITFKGAGRDVTAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNI 180

Query: 181 TFKNTTPVPAPGAIGKQAVAFRISADTAAFFGCRFLGAQDTLYDHLGRHYYKDCYIEGSV 240
           +F NT P P PG  G QAVAFRIS D A F GC F GAQDTL D  GRHY+K+CYIEGS+
Sbjct: 181 SFTNTAPAPLPGMQGWQAVAFRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSI 240

Query: 241 DFIFGNGLSLFEGCHVHAIAQYTGALTAQGRSSLLEDTGFSFVNCRAFDS---------- 300
           DFIFGNG S+++ C +H+IA   G++ A GR+   E TGF+FV CR   +          
Sbjct: 241 DFIFGNGRSMYKDCELHSIASRFGSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMG 300

Query: 301 --KRIVF-----------------------GMTVFYGQYKCTGEGASFAGRVSWSRELTD 344
              RIV+                         T F+G Y C G GA+    VSW+R L  
Sbjct: 301 QYSRIVYAYTYFDALVAHGGWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDY 357

[Close]

BLAST of Spg022435 vs. ExPASy Swiss-Prot
Match: Q9ZQA3 (Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 7.2e-59
Identity = 128/326 (39.26%), Postives = 183/326 (56.13%), Query Frame = 0


Query: 58  KFVGSLKHSVFRTAKNKLFPSYTLHVAKNPAAGDFTSIQDAVDSLPFINLVRVVIKVHAG 117
           K+   L+H      K  L  +  LH       G+F+++Q A+D +P ++  + +I V++G
Sbjct: 76  KWTSRLRHQY----KTSLVLTVDLH-----GCGNFSNVQSAIDVVPDLSSSKTLIIVNSG 135

Query: 118 VYTEKVNIPPLKSFITIEGAGADKTIVQWGDTAQTPGPKGQPMGTYNSATFAVNSPYFIA 177
            Y EKV +   K+ + I+G G   T ++W DTA++ G       T +S +F V +  F A
Sbjct: 136 SYREKVTVNENKTNLVIQGRGYQNTSIEWNDTAKSAG------NTADSFSFVVFAANFTA 195

Query: 178 KNITFKNTTPVPAPGAIGKQAVAFRISADTAAFFGCRFLGAQDTLYDHLGRHYYKDCYIE 237
            NI+FKN  P P PG    QAVA RI  D AAF+GC F GAQDTL D  GRH++K+C+I+
Sbjct: 196 YNISFKNNAPEPDPGEADAQAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQ 255

Query: 238 GSVDFIFGNGLSLFEGCHVHAIAQ-----YTGALTAQGRSSLLEDTGFSFVNCRAFDSKR 297
           GS+DFIFGNG SL++ C +++IA+      TG++TAQGR S  E +GFSFVNC+   S  
Sbjct: 256 GSIDFIFGNGRSLYQDCTINSIAKGNTSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGE 315

Query: 298 IVFG----------------------------------MTVFYGQYKCTGEGASFAGRVS 345
           I+ G                                   TV +G++KC G GA +  RV 
Sbjct: 316 ILLGRAWGAYATVVFSNTYMSGIITPEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVL 375

[Close]

BLAST of Spg022435 vs. ExPASy Swiss-Prot
Match: O23038 (Probable pectinesterase 8 OS=Arabidopsis thaliana OX=3702 GN=PME8 PE=2 SV=2)

HSP 1 Score: 222.6 bits (566), Expect = 6.7e-57
Identity = 123/296 (41.55%), Postives = 171/296 (57.77%), Query Frame = 0


Query: 91  DFTSIQDAVDSLPFINLVRVVIKVHAGVYTEKVNIPPLKSFITIEGAGADKTIVQWGDTA 150
           +FT++Q AVD++   +  R VI +++G+Y EKV IP  K  IT++G G D T + W DTA
Sbjct: 100 NFTTVQSAVDAVGNFSQRRNVIWINSGMYYEKVVIPKTKPNITLQGQGFDITAIAWNDTA 159

Query: 151 QTPGPKGQPMGTYNSATFAVNSPYFIAKNITFKNTTPVPAPGAIGKQAVAFRISADTAAF 210
            +        GT+  AT  V    F+AKNI+F N  P+P PG +G QAVA RI+ D +AF
Sbjct: 160 YSAN------GTFYCATVQVFGSQFVAKNISFMNVAPIPKPGDVGAQAVAIRIAGDESAF 219

Query: 211 FGCRFLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNGLSLFEGCHVHAIAQ--------Y 270
            GC F GAQDTL+D  GRHY+KDCYI+GS+DFIFGN  SL++ C + ++A          
Sbjct: 220 VGCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNAKSLYQDCRIISMANQLSPGSKAV 279

Query: 271 TGALTAQGRSSLLEDTGFSFVNC-----------RAF-DSKRIVF--------------- 330
            GA+TA GRSS  E++GFSFVNC           RA+    R+VF               
Sbjct: 280 NGAVTANGRSSKDENSGFSFVNCTIGGTGHVWLGRAWRPYSRVVFVSTTMTDVIAPEGWN 339

Query: 331 -------GMTVFYGQYKCTGEGASFAGRVSWSRELTDEEAKPFISLSFIDGSEWIK 345
                    T+FYG+Y C+G GA  + R  + ++L + +    I+ SFIDG +W++
Sbjct: 340 NFNDPSRDATIFYGEYNCSGPGADMSKRAPYVQKLNETQVALLINTSFIDGDQWLQ 389

[Close]

BLAST of Spg022435 vs. ExPASy Swiss-Prot
Match: Q9FM79 (Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 6.3e-55
Identity = 118/299 (39.46%), Postives = 161/299 (53.85%), Query Frame = 0


Query: 83  VAKNPAAGDFTSIQDAVDSLPFINLVRVVIKVHAGVYTEKVNIPPLKSFITIEG--AGAD 142
           V      GD  ++Q AVD +P  N  RV I +  G+Y EKV +P  K +I+  G  + A 
Sbjct: 83  VVDKNGGGDSVTVQGAVDMVPDSNSQRVKIFILPGIYREKVIVPKSKPYISFIGNESYAG 142

Query: 143 KTIVQWGDTAQTPGPKGQPMGTYNSATFAVNSPYFIAKNITFKNTTPVPAPGAIGKQAVA 202
            T++ W D A   G  G+ +GTY +A+ ++ S +F A  ITF+NT  V   G  G+QAVA
Sbjct: 143 DTVISWSDKASDLGCDGKELGTYRTASVSIESDFFCATAITFENTV-VAEAGEQGRQAVA 202

Query: 203 FRISADTAAFFGCRFLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNGLSLFEGCHVHAIA 262
            RI  D A F+  R LG+QDTL+D  G HY+  CYI+G+VDFIFGN  SL++ C +H+ A
Sbjct: 203 LRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSLYQDCDIHSTA 262

Query: 263 QYTGALTAQGRSSLLEDTGFSFVNCRAFDSKRIVFGMT---------------------- 322
           +  GA+ A  R S  EDTGFSFVNC    + +I  G                        
Sbjct: 263 KRYGAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRTVYSNCFIADIITPVG 322

Query: 323 ------------VFYGQYKCTGEGASFAGRVSWSRELTDEEAKPFISLSFIDGSEWIKI 346
                       V +G+Y C G GA   GRV WS+ LT +E KPF+   FI G +W+++
Sbjct: 323 WSDWKHPERQRKVMFGEYNCRGRGAERGGRVPWSKTLTRDEVKPFLGREFIYGDQWLRL 380

[Close]

BLAST of Spg022435 vs. ExPASy TrEMBL
Match: A0A6J1CVJ2 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111014609 PE=4 SV=1)

HSP 1 Score: 627.9 bits (1618), Expect = 2.6e-176
Identity = 318/379 (83.91%), Postives = 329/379 (86.81%), Query Frame = 0


Query: 1   MSKLQPPFTLSLLLLLLLLTQTHCHTKGLKPRKSARSALPKNSTKTQFSEQQFMKWVKFV 60
           MS+LQP   L LL L L +TQ+HCHTKG++PR   RSALPKNST TQFSEQQFMKWVKFV
Sbjct: 1   MSRLQPLLLLLLLSLSLSVTQSHCHTKGIRPRNEVRSALPKNSTNTQFSEQQFMKWVKFV 60

Query: 61  GSLKHSVFRTAKNKLFPSYTLHVAKNPAAGDFTSIQDAVDSLPFINLVRVVIKVHAGVYT 120
           G+LKHSVFRTAKNK+FPSYTLHVAKNPAAGDFTSIQDAVDSLPFINLVRVVIKVHAGVYT
Sbjct: 61  GTLKHSVFRTAKNKIFPSYTLHVAKNPAAGDFTSIQDAVDSLPFINLVRVVIKVHAGVYT 120

Query: 121 EKVNIPPLKSFITIEGAGADKTIVQWGDTAQTPGPKGQPMGTYNSATFAVNSPYFIAKNI 180
           EKVNIPPLKSFITIEGAGADKTIVQWGDTAQTPGPKGQPMGTYNSATFAVNSPYFIAKNI
Sbjct: 121 EKVNIPPLKSFITIEGAGADKTIVQWGDTAQTPGPKGQPMGTYNSATFAVNSPYFIAKNI 180

Query: 181 TFKNTTPVPAPGAIGKQAVAFRISADTAAFFGCRFLGAQDTLYDHLGRHYYKDCYIEGSV 240
           TFKNTTPVPAPGAIGKQAVAFRISADTAAFFGC+FLGAQDTLYDHLGRHYYKDCYIEGSV
Sbjct: 181 TFKNTTPVPAPGAIGKQAVAFRISADTAAFFGCKFLGAQDTLYDHLGRHYYKDCYIEGSV 240

Query: 241 DFIFGNGLSLFEGCHVHAIAQYTGALTAQGRSSLLEDTGFSFVNCRAFDS---------- 300
           DFIFGNGLSLFEGCHVHAIAQYTGALTAQGRSSLLEDTGFSFVNC+   S          
Sbjct: 241 DFIFGNGLSLFEGCHVHAIAQYTGALTAQGRSSLLEDTGFSFVNCKVTGSGALYLGRAWG 300

Query: 301 --KRIVFG----------------------MTVFYGQYKCTGEGASFAGRVSWSRELTDE 346
              R+VF                       MTVFYGQYKCTGEGAS+AGRVSWSRELTDE
Sbjct: 301 PFSRVVFAYTYMDNIIIPKGWYNWGDPNREMTVFYGQYKCTGEGASYAGRVSWSRELTDE 360

[Close]

BLAST of Spg022435 vs. ExPASy TrEMBL
Match: A0A6J1EB74 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111432428 PE=4 SV=1)

HSP 1 Score: 620.2 bits (1598), Expect = 5.4e-174
Identity = 316/382 (82.72%), Postives = 327/382 (85.60%), Query Frame = 0


Query: 1   MSKLQPPFTLS---LLLLLLLLTQTHCHTKGLKPRKSARSALPKNSTKTQFSEQQFMKWV 60
           MSKLQPPF  S   LLLLLLLLTQTHCHTKG++P+KS R+ LPKNSTKTQFSEQQFMKWV
Sbjct: 1   MSKLQPPFAFSLLLLLLLLLLLTQTHCHTKGIRPKKSPRTILPKNSTKTQFSEQQFMKWV 60

Query: 61  KFVGSLKHSVFRTAKNKLFPSYTLHVAKNPAAGDFTSIQDAVDSLPFINLVRVVIKVHAG 120
           KFVGSLKHSVFRTAKNKLFPSYTLHVAKNPAAGDFTSIQDA+DSLPFINL+RVVIKVHAG
Sbjct: 61  KFVGSLKHSVFRTAKNKLFPSYTLHVAKNPAAGDFTSIQDAIDSLPFINLIRVVIKVHAG 120

Query: 121 VYTEKVNIPPLKSFITIEGAGADKTIVQWGDTAQTPGPKGQPMGTYNSATFAVNSPYFIA 180
           VY EKV IPP KSFITIEGAGA KTIVQWGDTAQT GPKGQP+GTYNSATFAVNSPYFIA
Sbjct: 121 VYKEKVIIPPFKSFITIEGAGAAKTIVQWGDTAQTRGPKGQPLGTYNSATFAVNSPYFIA 180

Query: 181 KNITFKNTTPVPAPGAIGKQAVAFRISADTAAFFGCRFLGAQDTLYDHLGRHYYKDCYIE 240
           KNITFKNTTPVP PGAIGKQAVAFRIS DTAAF+GCRFLGAQDTLYDHLGRHYYKDCYIE
Sbjct: 181 KNITFKNTTPVPPPGAIGKQAVAFRISGDTAAFYGCRFLGAQDTLYDHLGRHYYKDCYIE 240

Query: 241 GSVDFIFGNGLSLFEGCHVHAIAQYTGALTAQGRSSLLEDTGFSFVNCRAFDS------- 300
           GSVDFIFGNGLSLFEGCHVHAIA+YTGALTAQGRSSLLEDTGFSFVNC+   S       
Sbjct: 241 GSVDFIFGNGLSLFEGCHVHAIARYTGALTAQGRSSLLEDTGFSFVNCKVTGSGALYLGR 300

Query: 301 -----KRIVFG----------------------MTVFYGQYKCTGEGASFAGRVSWSREL 346
                 R+VF                       MTVFYGQYKCTGEG  FAGRVSWSREL
Sbjct: 301 AWGPFSRVVFAYTYMDNIIIPKGWYNWGDPNREMTVFYGQYKCTGEGGRFAGRVSWSREL 360

[Close]

BLAST of Spg022435 vs. ExPASy TrEMBL
Match: A0A6J1KH39 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111495196 PE=4 SV=1)

HSP 1 Score: 616.3 bits (1588), Expect = 7.7e-173
Identity = 314/382 (82.20%), Postives = 326/382 (85.34%), Query Frame = 0


Query: 1   MSKLQPPFTLS---LLLLLLLLTQTHCHTKGLKPRKSARSALPKNSTKTQFSEQQFMKWV 60
           MSKLQPPF  S   LLLLLLLLTQTHCHTKG++P+KS R+ LPKNSTKTQFSEQQFMKWV
Sbjct: 1   MSKLQPPFAFSLLLLLLLLLLLTQTHCHTKGIRPKKSPRTILPKNSTKTQFSEQQFMKWV 60

Query: 61  KFVGSLKHSVFRTAKNKLFPSYTLHVAKNPAAGDFTSIQDAVDSLPFINLVRVVIKVHAG 120
           KFVGSLKHSVFRTAKNKLFPSYTLHVAK PAAGDFTSIQDA+DSLPFINL+RVVIKVHAG
Sbjct: 61  KFVGSLKHSVFRTAKNKLFPSYTLHVAKKPAAGDFTSIQDAIDSLPFINLIRVVIKVHAG 120

Query: 121 VYTEKVNIPPLKSFITIEGAGADKTIVQWGDTAQTPGPKGQPMGTYNSATFAVNSPYFIA 180
           VY EKV IPP KSFITIEGAGA KTIVQWGDTAQTPGPKGQP+GTYNSATFAVNS YFIA
Sbjct: 121 VYKEKVIIPPFKSFITIEGAGAAKTIVQWGDTAQTPGPKGQPLGTYNSATFAVNSLYFIA 180

Query: 181 KNITFKNTTPVPAPGAIGKQAVAFRISADTAAFFGCRFLGAQDTLYDHLGRHYYKDCYIE 240
           KNITFKNTTPVP PGA+GKQAVAFRIS DTAAF+GCRFLGAQDTLYDHLGRHYYKDCYIE
Sbjct: 181 KNITFKNTTPVPPPGAMGKQAVAFRISGDTAAFYGCRFLGAQDTLYDHLGRHYYKDCYIE 240

Query: 241 GSVDFIFGNGLSLFEGCHVHAIAQYTGALTAQGRSSLLEDTGFSFVNCRAFDS------- 300
           GSVDFIFGNGLSLFEGCHVHAIA+YTGALTAQGRSSLLEDTGFSFVNC+   S       
Sbjct: 241 GSVDFIFGNGLSLFEGCHVHAIARYTGALTAQGRSSLLEDTGFSFVNCKVTGSGALYLGR 300

Query: 301 -----KRIVFG----------------------MTVFYGQYKCTGEGASFAGRVSWSREL 346
                 R+VF                       MTVFYGQYKCTGEG  FAGRVSWSREL
Sbjct: 301 AWGPFSRVVFAYTYMDNIIIPKGWYNWGDPNREMTVFYGQYKCTGEGGRFAGRVSWSREL 360

[Close]

BLAST of Spg022435 vs. ExPASy TrEMBL
Match: A0A0A0LWA8 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G575010 PE=4 SV=1)

HSP 1 Score: 614.0 bits (1582), Expect = 3.8e-172
Identity = 313/386 (81.09%), Postives = 330/386 (85.49%), Query Frame = 0


Query: 1   MSKLQPPFTLSLLLLLLL-------LTQTHCHTKGLKPRKSARSALPKNSTKTQFSEQQF 60
           MSKL P  +L LLLLLLL        TQTHCHTKGLKP+KS+++ LP NSTKTQFSEQQF
Sbjct: 1   MSKLHPHPSLLLLLLLLLFLLLSLSFTQTHCHTKGLKPKKSSKNFLPNNSTKTQFSEQQF 60

Query: 61  MKWVKFVGSLKHSVFRTAKNKLFPSYTLHVAKNPAAGDFTSIQDAVDSLPFINLVRVVIK 120
           +KWVKFVGSL+HSVF+TAKNKLFPS+TLHVAKNPA+GDFTSIQDA+DSLPFINLVRVVIK
Sbjct: 61  LKWVKFVGSLRHSVFKTAKNKLFPSFTLHVAKNPASGDFTSIQDAIDSLPFINLVRVVIK 120

Query: 121 VHAGVYTEKVNIPPLKSFITIEGAGADKTIVQWGDTAQTPGPKGQPMGTYNSATFAVNSP 180
           VHAGVYTEKVNIPPLKSFITIEGAGA+KTI+QWGDTAQTPG  GQPMGTYNSATFAVNSP
Sbjct: 121 VHAGVYTEKVNIPPLKSFITIEGAGAEKTIIQWGDTAQTPGSNGQPMGTYNSATFAVNSP 180

Query: 181 YFIAKNITFKNTTPVPAPGAIGKQAVAFRISADTAAFFGCRFLGAQDTLYDHLGRHYYKD 240
           YFIAKNITFKNTTPVPAPGAIGKQAVAFRISADTAAFFGCRFLGAQDTLYDHLGRHYYKD
Sbjct: 181 YFIAKNITFKNTTPVPAPGAIGKQAVAFRISADTAAFFGCRFLGAQDTLYDHLGRHYYKD 240

Query: 241 CYIEGSVDFIFGNGLSLFEGCHVHAIAQYTGALTAQGRSSLLEDTGFSFVNCRAFDS--- 300
           CYIEGSVDFIFGNGLSLFEGCHVHAIAQYTGALTAQGRSSLLEDTGFSFV C+   S   
Sbjct: 241 CYIEGSVDFIFGNGLSLFEGCHVHAIAQYTGALTAQGRSSLLEDTGFSFVKCKVTGSGAL 300

Query: 301 ---------KRIVFG----------------------MTVFYGQYKCTGEGASFAGRVSW 346
                     R+VF                       MTVFYGQYKCTG+GASFAGRVSW
Sbjct: 301 YLGRAWGPFSRVVFAYTYMDNIIIPKGWYNWGDPNREMTVFYGQYKCTGDGASFAGRVSW 360

[Close]

BLAST of Spg022435 vs. ExPASy TrEMBL
Match: A0A6J1JJR5 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111485210 PE=4 SV=1)

HSP 1 Score: 606.7 bits (1563), Expect = 6.1e-170
Identity = 315/385 (81.82%), Postives = 326/385 (84.68%), Query Frame = 0


Query: 1   MSKLQ---PPFTLSLLLLLLL---LTQTHCHTKGLKPRKSARSALPKNSTKTQFSEQQFM 60
           MS+LQ   PP  L L+L+LLL   LT THCHTKGLKPR S ++ALP+NST TQFSEQQFM
Sbjct: 2   MSRLQRPPPPPLLVLVLVLLLSQSLTLTHCHTKGLKPRSSPKTALPENSTTTQFSEQQFM 61

Query: 61  KWVKFVGSLKHSVFRTAKNKLFPSYTLHVAKNPAAGDFTSIQDAVDSLPFINLVRVVIKV 120
           KWVKFVGSL+HSVFRTAKN LF SYTLHVAKNPA GDFTSIQDAVDSLP INLVRVVIKV
Sbjct: 62  KWVKFVGSLRHSVFRTAKNNLFSSYTLHVAKNPAVGDFTSIQDAVDSLPSINLVRVVIKV 121

Query: 121 HAGVYTEKVNIPPLKSFITIEGAGADKTIVQWGDTAQTPGPKGQPMGTYNSATFAVNSPY 180
           H GVYTEKVNIPPLKSFITIEGAGADKTIVQWGDTAQTPGPKGQPMGTYNSATFAVNSPY
Sbjct: 122 HEGVYTEKVNIPPLKSFITIEGAGADKTIVQWGDTAQTPGPKGQPMGTYNSATFAVNSPY 181

Query: 181 FIAKNITFKNTTPVPAPGAIGKQAVAFRISADTAAFFGCRFLGAQDTLYDHLGRHYYKDC 240
           FIAKNITFKNTTPVPAPGAIGKQAVAFRISADTAAF+GCRFLGAQDTLYDHLGRHYYKDC
Sbjct: 182 FIAKNITFKNTTPVPAPGAIGKQAVAFRISADTAAFYGCRFLGAQDTLYDHLGRHYYKDC 241

Query: 241 YIEGSVDFIFGNGLSLFEGCHVHAIAQYTGALTAQGRSSLLEDTGFSFVNCRAFDS---- 300
           YIEGSVDFIFGNGLSLFEGCHVHAIAQ  GALTAQGRSSLLEDTGFSFVNC+   S    
Sbjct: 242 YIEGSVDFIFGNGLSLFEGCHVHAIAQNAGALTAQGRSSLLEDTGFSFVNCKVTGSGALY 301

Query: 301 --------KRIVFG----------------------MTVFYGQYKCTGEGASFAGRVSWS 346
                    R+VF                       MTVFYGQYKCTGEGASFAGRVSWS
Sbjct: 302 LGRAWGPFSRVVFAYTYMDNIILPKGWYNWGDPSREMTVFYGQYKCTGEGASFAGRVSWS 361

[Close]

BLAST of Spg022435 vs. TAIR 10
Match: AT5G19730.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 509.2 bits (1310), Expect = 2.6e-144
Identity = 255/374 (68.18%), Postives = 288/374 (77.01%), Query Frame = 0


Query: 6   PPFTLSLLLLLLLLTQTHCHTKGLKPRKSARSALPKNSTKTQFSEQQFMKWVKFVGSLKH 65
           P   L LL++LL  TQT CHTKGL+ R   +  +   S +TQ  E +FMKWV+FVGSLKH
Sbjct: 10  PNLFLLLLVILLCSTQTQCHTKGLRLRPRNQKNMNTTSDRTQNPEDEFMKWVRFVGSLKH 69

Query: 66  SVFRTAKNKLFPSYTLHVAKNPAAGDFTSIQDAVDSLPFINLVRVVIKVHAGVYTEKVNI 125
           SVF+ AKNKLFPSYTL V K    GDFT IQDA+DSLP IN VRVVIKVHAGVY EKV+I
Sbjct: 70  SVFKAAKNKLFPSYTLTVHKKSNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSI 129

Query: 126 PPLKSFITIEGAGADKTIVQWGDTAQTPGPKGQPMGTYNSATFAVNSPYFIAKNITFKNT 185
           PPLK+FITIEG GA+KT V+WGDTAQTP  KG PMGTYNSA+FAVNSP+F+AKNITF+NT
Sbjct: 130 PPLKAFITIEGEGAEKTTVEWGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNT 189

Query: 186 TPVPAPGAIGKQAVAFRISADTAAFFGCRFLGAQDTLYDHLGRHYYKDCYIEGSVDFIFG 245
           TPVP PGA+GKQAVA R+SAD AAFFGCR LGAQDTLYDHLGRHYYKDCYIEGSVDFIFG
Sbjct: 190 TPVPLPGAVGKQAVALRVSADNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFG 249

Query: 246 NGLSLFEGCHVHAIAQYTGALTAQGRSSLLEDTGFSFVNCRAFDS------------KRI 305
           N LSL+EGCHVHAIA   GA+TAQGRSS+LEDTGFSFV C+   +             R+
Sbjct: 250 NALSLYEGCHVHAIADKLGAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRV 309

Query: 306 VFG----------------------MTVFYGQYKCTGEGASFAGRVSWSRELTDEEAKPF 346
           VF                       MTVFYGQYKCTG GA++ GRV+W+RELTDEEAKPF
Sbjct: 310 VFAYTYMDNIILPRGWYNWGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPF 369

[Close]

BLAST of Spg022435 vs. TAIR 10
Match: AT5G47500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 248.4 bits (633), Expect = 8.1e-66
Identity = 150/378 (39.68%), Postives = 192/378 (50.79%), Query Frame = 0


Query: 1   MSKLQPPFTLSLLLLLLLLTQTHCHTKGLKPRKSARSALPKNSTKTQFSEQQFMKWVKFV 60
           M++L         + LLL    HC    L  R S  +A   ++   Q       KW   V
Sbjct: 1   MAQLTNSLNYLFSVSLLLFVSFHC----LCFRFSLVAACSNSTDDQQIQHHHHRKW---V 60

Query: 61  GSLKHSVFRTAKNKLFPSYTLHVAKNPAAGDFTSIQDAVDSLPFINLVRVVIKVHAGVYT 120
           G   H V   + N                  F S+QDAVDS+P  N   + IK+  G Y 
Sbjct: 61  GPSGHKVITVSLN--------------GHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYR 120

Query: 121 EKVNIPPLKSFITIEGAGADKTIVQWGDTAQTPGPKGQPMGTYNSATFAVNSPYFIAKNI 180
           EKV +P  K +IT +GAG D T ++W D A   G  GQ + TY +A+  V + YF A+NI
Sbjct: 121 EKVVVPATKPYITFKGAGRDVTAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNI 180

Query: 181 TFKNTTPVPAPGAIGKQAVAFRISADTAAFFGCRFLGAQDTLYDHLGRHYYKDCYIEGSV 240
           +F NT P P PG  G QAVAFRIS D A F GC F GAQDTL D  GRHY+K+CYIEGS+
Sbjct: 181 SFTNTAPAPLPGMQGWQAVAFRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSI 240

Query: 241 DFIFGNGLSLFEGCHVHAIAQYTGALTAQGRSSLLEDTGFSFVNCRAFDS---------- 300
           DFIFGNG S+++ C +H+IA   G++ A GR+   E TGF+FV CR   +          
Sbjct: 241 DFIFGNGRSMYKDCELHSIASRFGSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMG 300

Query: 301 --KRIVF-----------------------GMTVFYGQYKCTGEGASFAGRVSWSRELTD 344
              RIV+                         T F+G Y C G GA+    VSW+R L  
Sbjct: 301 QYSRIVYAYTYFDALVAHGGWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDY 357

[Close]

BLAST of Spg022435 vs. TAIR 10
Match: AT2G36710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 229.2 bits (583), Expect = 5.1e-60
Identity = 128/326 (39.26%), Postives = 183/326 (56.13%), Query Frame = 0


Query: 58  KFVGSLKHSVFRTAKNKLFPSYTLHVAKNPAAGDFTSIQDAVDSLPFINLVRVVIKVHAG 117
           K+   L+H      K  L  +  LH       G+F+++Q A+D +P ++  + +I V++G
Sbjct: 76  KWTSRLRHQY----KTSLVLTVDLH-----GCGNFSNVQSAIDVVPDLSSSKTLIIVNSG 135

Query: 118 VYTEKVNIPPLKSFITIEGAGADKTIVQWGDTAQTPGPKGQPMGTYNSATFAVNSPYFIA 177
            Y EKV +   K+ + I+G G   T ++W DTA++ G       T +S +F V +  F A
Sbjct: 136 SYREKVTVNENKTNLVIQGRGYQNTSIEWNDTAKSAG------NTADSFSFVVFAANFTA 195

Query: 178 KNITFKNTTPVPAPGAIGKQAVAFRISADTAAFFGCRFLGAQDTLYDHLGRHYYKDCYIE 237
            NI+FKN  P P PG    QAVA RI  D AAF+GC F GAQDTL D  GRH++K+C+I+
Sbjct: 196 YNISFKNNAPEPDPGEADAQAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQ 255

Query: 238 GSVDFIFGNGLSLFEGCHVHAIAQ-----YTGALTAQGRSSLLEDTGFSFVNCRAFDSKR 297
           GS+DFIFGNG SL++ C +++IA+      TG++TAQGR S  E +GFSFVNC+   S  
Sbjct: 256 GSIDFIFGNGRSLYQDCTINSIAKGNTSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGE 315

Query: 298 IVFG----------------------------------MTVFYGQYKCTGEGASFAGRVS 345
           I+ G                                   TV +G++KC G GA +  RV 
Sbjct: 316 ILLGRAWGAYATVVFSNTYMSGIITPEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVL 375

[Close]

BLAST of Spg022435 vs. TAIR 10
Match: AT1G05310.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 222.6 bits (566), Expect = 4.8e-58
Identity = 123/296 (41.55%), Postives = 171/296 (57.77%), Query Frame = 0


Query: 91  DFTSIQDAVDSLPFINLVRVVIKVHAGVYTEKVNIPPLKSFITIEGAGADKTIVQWGDTA 150
           +FT++Q AVD++   +  R VI +++G+Y EKV IP  K  IT++G G D T + W DTA
Sbjct: 100 NFTTVQSAVDAVGNFSQRRNVIWINSGMYYEKVVIPKTKPNITLQGQGFDITAIAWNDTA 159

Query: 151 QTPGPKGQPMGTYNSATFAVNSPYFIAKNITFKNTTPVPAPGAIGKQAVAFRISADTAAF 210
            +        GT+  AT  V    F+AKNI+F N  P+P PG +G QAVA RI+ D +AF
Sbjct: 160 YSAN------GTFYCATVQVFGSQFVAKNISFMNVAPIPKPGDVGAQAVAIRIAGDESAF 219

Query: 211 FGCRFLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNGLSLFEGCHVHAIAQ--------Y 270
            GC F GAQDTL+D  GRHY+KDCYI+GS+DFIFGN  SL++ C + ++A          
Sbjct: 220 VGCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNAKSLYQDCRIISMANQLSPGSKAV 279

Query: 271 TGALTAQGRSSLLEDTGFSFVNC-----------RAF-DSKRIVF--------------- 330
            GA+TA GRSS  E++GFSFVNC           RA+    R+VF               
Sbjct: 280 NGAVTANGRSSKDENSGFSFVNCTIGGTGHVWLGRAWRPYSRVVFVSTTMTDVIAPEGWN 339

Query: 331 -------GMTVFYGQYKCTGEGASFAGRVSWSRELTDEEAKPFISLSFIDGSEWIK 345
                    T+FYG+Y C+G GA  + R  + ++L + +    I+ SFIDG +W++
Sbjct: 340 NFNDPSRDATIFYGEYNCSGPGADMSKRAPYVQKLNETQVALLINTSFIDGDQWLQ 389

[Close]

BLAST of Spg022435 vs. TAIR 10
Match: AT5G55590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 216.1 bits (549), Expect = 4.5e-56
Identity = 118/299 (39.46%), Postives = 161/299 (53.85%), Query Frame = 0


Query: 83  VAKNPAAGDFTSIQDAVDSLPFINLVRVVIKVHAGVYTEKVNIPPLKSFITIEG--AGAD 142
           V      GD  ++Q AVD +P  N  RV I +  G+Y EKV +P  K +I+  G  + A 
Sbjct: 83  VVDKNGGGDSVTVQGAVDMVPDSNSQRVKIFILPGIYREKVIVPKSKPYISFIGNESYAG 142

Query: 143 KTIVQWGDTAQTPGPKGQPMGTYNSATFAVNSPYFIAKNITFKNTTPVPAPGAIGKQAVA 202
            T++ W D A   G  G+ +GTY +A+ ++ S +F A  ITF+NT  V   G  G+QAVA
Sbjct: 143 DTVISWSDKASDLGCDGKELGTYRTASVSIESDFFCATAITFENTV-VAEAGEQGRQAVA 202

Query: 203 FRISADTAAFFGCRFLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNGLSLFEGCHVHAIA 262
            RI  D A F+  R LG+QDTL+D  G HY+  CYI+G+VDFIFGN  SL++ C +H+ A
Sbjct: 203 LRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSLYQDCDIHSTA 262

Query: 263 QYTGALTAQGRSSLLEDTGFSFVNCRAFDSKRIVFGMT---------------------- 322
           +  GA+ A  R S  EDTGFSFVNC    + +I  G                        
Sbjct: 263 KRYGAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRTVYSNCFIADIITPVG 322

Query: 323 ------------VFYGQYKCTGEGASFAGRVSWSRELTDEEAKPFISLSFIDGSEWIKI 346
                       V +G+Y C G GA   GRV WS+ LT +E KPF+   FI G +W+++
Sbjct: 323 WSDWKHPERQRKVMFGEYNCRGRGAERGGRVPWSKTLTRDEVKPFLGREFIYGDQWLRL 380

The following BLAST results are available for this feature:

BLAST of Spg022435 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022145102.1	5.3e-176	83.91	probable pectinesterase 53 [Momordica charantia]	[more]
KAG6588003.1	1.0e-174	82.98	putative pectinesterase 53, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7021897.1	1.3e-174	82.77	putative pectinesterase 53, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023529306.1	2.2e-174	82.77	probable pectinesterase 53 [Cucurbita pepo subsp. pepo]	[more]
XP_022925076.1	1.1e-173	82.72	probable pectinesterase 53 [Cucurbita moschata]	[more]

BLAST of Spg022435 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VYZ3	3.6e-143	68.18	Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1	[more]
Q8LPF3	1.1e-64	39.68	Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1	[more]
Q9ZQA3	7.2e-59	39.26	Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1	[more]
O23038	6.7e-57	41.55	Probable pectinesterase 8 OS=Arabidopsis thaliana OX=3702 GN=PME8 PE=2 SV=2	[more]
Q9FM79	6.3e-55	39.46	Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1	[more]

BLAST of Spg022435 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CVJ2	2.6e-176	83.91	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111014609 PE=4 SV=1	[more]
A0A6J1EB74	5.4e-174	82.72	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111432428 PE=4 SV=1	[more]
A0A6J1KH39	7.7e-173	82.20	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111495196 PE=4 SV=1	[more]
A0A0A0LWA8	3.8e-172	81.09	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G575010 PE=4 SV=1	[more]
A0A6J1JJR5	6.1e-170	81.82	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111485210 PE=4 SV=1	[more]

BLAST of Spg022435 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G19730.1	2.6e-144	68.18	Pectin lyase-like superfamily protein	[more]
AT5G47500.1	8.1e-66	39.68	Pectin lyase-like superfamily protein	[more]
AT2G36710.1	5.1e-60	39.26	Pectin lyase-like superfamily protein	[more]
AT1G05310.1	4.8e-58	41.55	Pectin lyase-like superfamily protein	[more]
AT5G55590.1	4.5e-56	39.46	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 77..345 e-value: 1.5E-79 score: 269.7
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 83..288 e-value: 1.4E-51 score: 175.0
None	No IPR available	PANTHER	PTHR31321:SF103	PECTINESTERASE	coord: 297..344
None	No IPR available	PANTHER	PTHR31321:SF103	PECTINESTERASE	coord: 14..287
None	No IPR available	PANTHER	PTHR31321	ACYL-COA THIOESTER HYDROLASE YBHC-RELATED	coord: 297..344 coord: 14..287
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 236..245
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 76..344

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg022435.1	Spg022435.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0045490	pectin catabolic process
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity