Spg022328 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg022328 vs. NCBI nr
Match: XP_031738141.1 (phosphomethylethanolamine N-methyltransferase isoform X1 [Cucumis sativus])

HSP 1 Score: 855.1 bits (2208), Expect = 2.9e-244
Identity = 427/499 (85.57%), Postives = 456/499 (91.38%), Query Frame = 0

Query: 1   MASSAESAAPDREERERQKKYWIEHSSDLTIESMMVDSDSSELDSEERAEVISLLPPYEG 60
           MAS  +S A D EERERQKKYWIEHSSDLT+ESMMVDSDS ELDSEE AEVISLLPPYEG
Sbjct: 1   MASFVKSTA-DLEERERQKKYWIEHSSDLTMESMMVDSDSHELDSEETAEVISLLPPYEG 60

Query: 61  KTVAELGAGIGLFTAELAQRAAQVLAVDFIEEVIKKNEIINGHHKNVKFICADVTSPELD 120
           KTV ELGAGIG FT+ELAQRAAQV+AVDF+E+VI+KNE INGHHKNVKF CADVT  ELD
Sbjct: 61  KTVVELGAGIGRFTSELAQRAAQVIAVDFVEDVIRKNESINGHHKNVKFQCADVTFSELD 120

Query: 121 IAEESLDLIFSNWLLMYLSDEEVKILAERMIKWLKVGGYIFFREACFQHSENFDKKHDLS 180
           I E+S+D IFSN LLMYLSDEEVK+LAERMIKWLKVGGYIFFRE+CFQH EN DKK+D+S
Sbjct: 121 IHEDSVDFIFSNLLLMYLSDEEVKLLAERMIKWLKVGGYIFFRESCFQHYENSDKKNDVS 180

Query: 181 HYREPRFYSKVFKE-CYAKDDSGSVFELALLGCKPTGAYLRNKKSQNQICWIWQKVHSDD 240
           ++REPRFYSKVFKE C+ KDDS +VFEL+LLGCKPTG +  NKKSQNQICWIWQKV SD+
Sbjct: 181 YHREPRFYSKVFKELCFVKDDSENVFELSLLGCKPTGVF-GNKKSQNQICWIWQKVRSDN 240

Query: 241 DRGFQQFLDTVHYKSSGILRYERVFGHGFVSPGGLDTTKEFVAKLELKPGQKVLDVCCGI 300
           DRGFQ FLDTV YKSSGIL+YERVFGHGF+SPGGL+TTKEFVAKL+LKP Q+VLDV CGI
Sbjct: 241 DRGFQLFLDTVQYKSSGILKYERVFGHGFISPGGLETTKEFVAKLDLKPRQRVLDVGCGI 300

Query: 301 GGADFYMAENFDVEVVGIDLSVNMISLALERAIGLKCSVEFEVADCTKKTYPDHTFDVIY 360
           GGADFYMAENF VEVVGIDLSVNMISLALERAIGL CSVEFEVADCTKKTYPDHTFDVIY
Sbjct: 301 GGADFYMAENFSVEVVGIDLSVNMISLALERAIGLACSVEFEVADCTKKTYPDHTFDVIY 360

Query: 361 SRDAILHIQDKPALFRSFYKWLKPEGKLFITDYCRSAGTSSPEFAEYIKQGGYDLHDVKT 420
           SRD ILHIQDKP+LFRSFYKWLKP GKLFITDYCR+AGTSSPEFAEYIKQ GYDLHDVK 
Sbjct: 361 SRDTILHIQDKPSLFRSFYKWLKPGGKLFITDYCRNAGTSSPEFAEYIKQRGYDLHDVKA 420

Query: 421 YGQMIEDAGFSEVVSEDRTYQFIQLLQRELEAVEEQKDTFIHDFSEEGYNHIVSGWKAKR 480
           YGQMIEDAGF EVVSEDRT QFIQ+LQ+ELEAVEEQKD FI DFSEEGYN IV GWKAK 
Sbjct: 421 YGQMIEDAGFGEVVSEDRTNQFIQVLQQELEAVEEQKDRFILDFSEEGYNDIVCGWKAKL 480

Query: 481 NRGLSGEQRWGLFFAKKIN 499
           NR LSGEQRWGLFFAKKIN
Sbjct: 481 NRSLSGEQRWGLFFAKKIN 497

BLAST of Spg022328 vs. NCBI nr
Match: XP_031738143.1 (phosphomethylethanolamine N-methyltransferase isoform X2 [Cucumis sativus])

HSP 1 Score: 851.7 bits (2199), Expect = 3.3e-243
Identity = 421/487 (86.45%), Postives = 449/487 (92.20%), Query Frame = 0

Query: 13  EERERQKKYWIEHSSDLTIESMMVDSDSSELDSEERAEVISLLPPYEGKTVAELGAGIGL 72
           EERERQKKYWIEHSSDLT+ESMMVDSDS ELDSEE AEVISLLPPYEGKTV ELGAGIG 
Sbjct: 2   EERERQKKYWIEHSSDLTMESMMVDSDSHELDSEETAEVISLLPPYEGKTVVELGAGIGR 61

Query: 73  FTAELAQRAAQVLAVDFIEEVIKKNEIINGHHKNVKFICADVTSPELDIAEESLDLIFSN 132
           FT+ELAQRAAQV+AVDF+E+VI+KNE INGHHKNVKF CADVT  ELDI E+S+D IFSN
Sbjct: 62  FTSELAQRAAQVIAVDFVEDVIRKNESINGHHKNVKFQCADVTFSELDIHEDSVDFIFSN 121

Query: 133 WLLMYLSDEEVKILAERMIKWLKVGGYIFFREACFQHSENFDKKHDLSHYREPRFYSKVF 192
            LLMYLSDEEVK+LAERMIKWLKVGGYIFFRE+CFQH EN DKK+D+S++REPRFYSKVF
Sbjct: 122 LLLMYLSDEEVKLLAERMIKWLKVGGYIFFRESCFQHYENSDKKNDVSYHREPRFYSKVF 181

Query: 193 KE-CYAKDDSGSVFELALLGCKPTGAYLRNKKSQNQICWIWQKVHSDDDRGFQQFLDTVH 252
           KE C+ KDDS +VFEL+LLGCKPTG +  NKKSQNQICWIWQKV SD+DRGFQ FLDTV 
Sbjct: 182 KELCFVKDDSENVFELSLLGCKPTGVF-GNKKSQNQICWIWQKVRSDNDRGFQLFLDTVQ 241

Query: 253 YKSSGILRYERVFGHGFVSPGGLDTTKEFVAKLELKPGQKVLDVCCGIGGADFYMAENFD 312
           YKSSGIL+YERVFGHGF+SPGGL+TTKEFVAKL+LKP Q+VLDV CGIGGADFYMAENF 
Sbjct: 242 YKSSGILKYERVFGHGFISPGGLETTKEFVAKLDLKPRQRVLDVGCGIGGADFYMAENFS 301

Query: 313 VEVVGIDLSVNMISLALERAIGLKCSVEFEVADCTKKTYPDHTFDVIYSRDAILHIQDKP 372
           VEVVGIDLSVNMISLALERAIGL CSVEFEVADCTKKTYPDHTFDVIYSRD ILHIQDKP
Sbjct: 302 VEVVGIDLSVNMISLALERAIGLACSVEFEVADCTKKTYPDHTFDVIYSRDTILHIQDKP 361

Query: 373 ALFRSFYKWLKPEGKLFITDYCRSAGTSSPEFAEYIKQGGYDLHDVKTYGQMIEDAGFSE 432
           +LFRSFYKWLKP GKLFITDYCR+AGTSSPEFAEYIKQ GYDLHDVK YGQMIEDAGF E
Sbjct: 362 SLFRSFYKWLKPGGKLFITDYCRNAGTSSPEFAEYIKQRGYDLHDVKAYGQMIEDAGFGE 421

Query: 433 VVSEDRTYQFIQLLQRELEAVEEQKDTFIHDFSEEGYNHIVSGWKAKRNRGLSGEQRWGL 492
           VVSEDRT QFIQ+LQ+ELEAVEEQKD FI DFSEEGYN IV GWKAK NR LSGEQRWGL
Sbjct: 422 VVSEDRTNQFIQVLQQELEAVEEQKDRFILDFSEEGYNDIVCGWKAKLNRSLSGEQRWGL 481

Query: 493 FFAKKIN 499
           FFAKKIN
Sbjct: 482 FFAKKIN 487

BLAST of Spg022328 vs. NCBI nr
Match: KAA0058136.1 (phosphomethylethanolamine N-methyltransferase isoform X1 [Cucumis melo var. makuwa] >TYK28490.1 phosphomethylethanolamine N-methyltransferase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 850.1 bits (2195), Expect = 9.5e-243
Identity = 420/499 (84.17%), Postives = 456/499 (91.38%), Query Frame = 0

Query: 1   MASSAESAAPDREERERQKKYWIEHSSDLTIESMMVDSDSSELDSEERAEVISLLPPYEG 60
           MAS  +S   D  +R+RQKKYWIEHSSDLT+ES+MVDSDS ELDSEE+AEVISLLPPYEG
Sbjct: 1   MASFVKSTFSD--QRDRQKKYWIEHSSDLTMESVMVDSDSHELDSEEKAEVISLLPPYEG 60

Query: 61  KTVAELGAGIGLFTAELAQRAAQVLAVDFIEEVIKKNEIINGHHKNVKFICADVTSPELD 120
           KTV ELGAG+G FTAELAQRAAQV+AVDF+E+VI+KNE INGHHKNVKF+ ADVTSPELD
Sbjct: 61  KTVVELGAGVGRFTAELAQRAAQVIAVDFVEDVIRKNESINGHHKNVKFLWADVTSPELD 120

Query: 121 IAEESLDLIFSNWLLMYLSDEEVKILAERMIKWLKVGGYIFFREACFQHSENFDKKHDLS 180
           I E+S+D IFSN LLMYLSDEEVK+LAERMIKWLKVGGYIFFRE+CFQ SEN DKKHD+S
Sbjct: 121 IHEDSVDFIFSNLLLMYLSDEEVKLLAERMIKWLKVGGYIFFRESCFQDSENSDKKHDVS 180

Query: 181 HYREPRFYSKVFKE-CYAKDDSGSVFELALLGCKPTGAYLRNKKSQNQICWIWQKVHSDD 240
           ++REPRFYSKVFKE C+ KDDS + FEL+LL CKPTG +  NKKS+NQICWIWQKV SD+
Sbjct: 181 YHREPRFYSKVFKELCFVKDDSENAFELSLLDCKPTGVF-GNKKSRNQICWIWQKVRSDN 240

Query: 241 DRGFQQFLDTVHYKSSGILRYERVFGHGFVSPGGLDTTKEFVAKLELKPGQKVLDVCCGI 300
           DRGFQQFLDTV YKSSGIL+YERVFGHGF+SPGGL+TTKEFVAKL+LKP Q+VLDV CGI
Sbjct: 241 DRGFQQFLDTVQYKSSGILKYERVFGHGFISPGGLETTKEFVAKLDLKPRQRVLDVGCGI 300

Query: 301 GGADFYMAENFDVEVVGIDLSVNMISLALERAIGLKCSVEFEVADCTKKTYPDHTFDVIY 360
           GGADFYM ENF VEVVGIDLSVNMISLALERAIGL CSVEFEVADCTKKTYPDH+FDVIY
Sbjct: 301 GGADFYMVENFSVEVVGIDLSVNMISLALERAIGLSCSVEFEVADCTKKTYPDHSFDVIY 360

Query: 361 SRDAILHIQDKPALFRSFYKWLKPEGKLFITDYCRSAGTSSPEFAEYIKQGGYDLHDVKT 420
           SRD ILHIQDKP+LFRSFYKWLKP GKLFITDYC++AGTSSPEFAEYIKQ GYDLHDVKT
Sbjct: 361 SRDTILHIQDKPSLFRSFYKWLKPGGKLFITDYCKNAGTSSPEFAEYIKQRGYDLHDVKT 420

Query: 421 YGQMIEDAGFSEVVSEDRTYQFIQLLQRELEAVEEQKDTFIHDFSEEGYNHIVSGWKAKR 480
           YGQMIEDAGF EVVSEDRT QFIQ+LQRELEAVEEQKD FI DFSEEGYN I+ GWKAK 
Sbjct: 421 YGQMIEDAGFGEVVSEDRTNQFIQVLQRELEAVEEQKDRFILDFSEEGYNDIICGWKAKL 480

Query: 481 NRGLSGEQRWGLFFAKKIN 499
           NR LSGEQRWGLFFAKKIN
Sbjct: 481 NRSLSGEQRWGLFFAKKIN 496

BLAST of Spg022328 vs. NCBI nr
Match: KGN63873.1 (hypothetical protein Csa_014300 [Cucumis sativus])

HSP 1 Score: 815.5 bits (2105), Expect = 2.6e-232
Identity = 403/469 (85.93%), Postives = 431/469 (91.90%), Query Frame = 0

Query: 31  IESMMVDSDSSELDSEERAEVISLLPPYEGKTVAELGAGIGLFTAELAQRAAQVLAVDFI 90
           +ESMMVDSDS ELDSEE AEVISLLPPYEGKTV ELGAGIG FT+ELAQRAAQV+AVDF+
Sbjct: 1   MESMMVDSDSHELDSEETAEVISLLPPYEGKTVVELGAGIGRFTSELAQRAAQVIAVDFV 60

Query: 91  EEVIKKNEIINGHHKNVKFICADVTSPELDIAEESLDLIFSNWLLMYLSDEEVKILAERM 150
           E+VI+KNE INGHHKNVKF CADVT  ELDI E+S+D IFSN LLMYLSDEEVK+LAERM
Sbjct: 61  EDVIRKNESINGHHKNVKFQCADVTFSELDIHEDSVDFIFSNLLLMYLSDEEVKLLAERM 120

Query: 151 IKWLKVGGYIFFREACFQHSENFDKKHDLSHYREPRFYSKVFKE-CYAKDDSGSVFELAL 210
           IKWLKVGGYIFFRE+CFQH EN DKK+D+S++REPRFYSKVFKE C+ KDDS +VFEL+L
Sbjct: 121 IKWLKVGGYIFFRESCFQHYENSDKKNDVSYHREPRFYSKVFKELCFVKDDSENVFELSL 180

Query: 211 LGCKPTGAYLRNKKSQNQICWIWQKVHSDDDRGFQQFLDTVHYKSSGILRYERVFGHGFV 270
           LGCKPTG +  NKKSQNQICWIWQKV SD+DRGFQ FLDTV YKSSGIL+YERVFGHGF+
Sbjct: 181 LGCKPTGVF-GNKKSQNQICWIWQKVRSDNDRGFQLFLDTVQYKSSGILKYERVFGHGFI 240

Query: 271 SPGGLDTTKEFVAKLELKPGQKVLDVCCGIGGADFYMAENFDVEVVGIDLSVNMISLALE 330
           SPGGL+TTKEFVAKL+LKP Q+VLDV CGIGGADFYMAENF VEVVGIDLSVNMISLALE
Sbjct: 241 SPGGLETTKEFVAKLDLKPRQRVLDVGCGIGGADFYMAENFSVEVVGIDLSVNMISLALE 300

Query: 331 RAIGLKCSVEFEVADCTKKTYPDHTFDVIYSRDAILHIQDKPALFRSFYKWLKPEGKLFI 390
           RAIGL CSVEFEVADCTKKTYPDHTFDVIYSRD ILHIQDKP+LFRSFYKWLKP GKLFI
Sbjct: 301 RAIGLACSVEFEVADCTKKTYPDHTFDVIYSRDTILHIQDKPSLFRSFYKWLKPGGKLFI 360

Query: 391 TDYCRSAGTSSPEFAEYIKQGGYDLHDVKTYGQMIEDAGFSEVVSEDRTYQFIQLLQREL 450
           TDYCR+AGTSSPEFAEYIKQ GYDLHDVK YGQMIEDAGF EVVSEDRT QFIQ+LQ+EL
Sbjct: 361 TDYCRNAGTSSPEFAEYIKQRGYDLHDVKAYGQMIEDAGFGEVVSEDRTNQFIQVLQQEL 420

Query: 451 EAVEEQKDTFIHDFSEEGYNHIVSGWKAKRNRGLSGEQRWGLFFAKKIN 499
           EAVEEQKD FI DFSEEGYN IV GWKAK NR LSGEQRWGLFFAKKIN
Sbjct: 421 EAVEEQKDRFILDFSEEGYNDIVCGWKAKLNRSLSGEQRWGLFFAKKIN 468

BLAST of Spg022328 vs. NCBI nr
Match: XP_038878462.1 (phosphoethanolamine N-methyltransferase 1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 805.4 bits (2079), Expect = 2.7e-229
Identity = 396/497 (79.68%), Postives = 434/497 (87.32%), Query Frame = 0

Query: 1   MASSAESAAPDREERERQKKYWIEHSSDLTIESMMVDSDSSELDSEERAEVISLLPPYEG 60
           MAS  +S + DREERERQK YWI+HS+DL IESMMVDSDS  L SEE AEVISLLPPYEG
Sbjct: 1   MASFVKSTSSDREERERQKNYWIDHSADLNIESMMVDSDSHGLHSEETAEVISLLPPYEG 60

Query: 61  KTVAELGAGIGLFTAELAQRAAQVLAVDFIEEVIKKNEIINGHHKNVKFICADVTSPELD 120
           KTV ELGAGIG FTAELAQRAAQVLAVD IE+VIKKNE INGH+KNV+F+CADVTSPELD
Sbjct: 61  KTVVELGAGIGRFTAELAQRAAQVLAVDVIEDVIKKNESINGHNKNVRFLCADVTSPELD 120

Query: 121 IAEESLDLIFSNWLLMYLSDEEVKILAERMIKWLKVGGYIFFREACFQHSENFDKKHDLS 180
           I E+S+D IFSN  LMY SDEEVKILA+RMIKWLKVGGYIFFRE+CFQHSEN DKKHDLS
Sbjct: 121 IPEDSVDFIFSNLPLMYFSDEEVKILADRMIKWLKVGGYIFFRESCFQHSENSDKKHDLS 180

Query: 181 HYREPRFYSKVFKECYAKDDSGSVFELALLGCKPTGAYLRNKKSQNQICWIWQKVHSDDD 240
           HY EPRFYSKV KECYAKDDSG+VFEL+LLGCKPTG YL NK SQNQ+CWIWQK  S  +
Sbjct: 181 HYWEPRFYSKVLKECYAKDDSGNVFELSLLGCKPTGVYLGNKTSQNQVCWIWQKKKSSSE 240

Query: 241 -RGFQQFLDTVHYKSSGILRYERVFGHGFVSPGGLDTTKEFVAKLELKPGQKVLDVCCGI 300
            +GFQ+FLDT+ YK +GILRYERVFG GFVS GG+DTTKEFVA+LELK GQ+VLDV CGI
Sbjct: 241 GKGFQRFLDTIQYKCNGILRYERVFGPGFVSTGGIDTTKEFVAQLELKAGQRVLDVGCGI 300

Query: 301 GGADFYMAENFDVEVVGIDLSVNMISLALERAIGLKCSVEFEVADCTKKTYPDHTFDVIY 360
           GG DFYMAENF VEVVGIDLS+NMISLALERAIGLKC VEFEVADCTKK Y D+ FDVIY
Sbjct: 301 GGGDFYMAENFGVEVVGIDLSINMISLALERAIGLKCPVEFEVADCTKKVYADNIFDVIY 360

Query: 361 SRDAILHIQDKPALFRSFYKWLKPEGKLFITDYCRSAGTSSPEFAEYIKQGGYDLHDVKT 420
           SRD ILHIQDKPALFRSFYKWLKP GK+ I+DYC+S+G  SPEFAEYIKQ GYDLHDVK+
Sbjct: 361 SRDTILHIQDKPALFRSFYKWLKPGGKILISDYCKSSGIPSPEFAEYIKQRGYDLHDVKS 420

Query: 421 YGQMIEDAGFSEVVSEDRTYQFIQLLQRELEAVEEQKDTFIHDFSEEGYNHIVSGWKAKR 480
           YGQM+E+AGFS VV+EDRT QFI +LQREL  VE++K+ FIHDFSEE YN IV GWKAK 
Sbjct: 421 YGQMLEEAGFSNVVAEDRTDQFISVLQRELNTVEKEKEAFIHDFSEEDYNDIVGGWKAKL 480

Query: 481 NRGLSGEQRWGLFFAKK 497
           NR   GEQ+WGLF A K
Sbjct: 481 NRCWCGEQKWGLFIAHK 497

BLAST of Spg022328 vs. ExPASy Swiss-Prot
Match: Q944H0 (Phosphomethylethanolamine N-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=NMT2 PE=1 SV=2)

HSP 1 Score: 750.0 bits (1935), Expect = 1.8e-215
Identity = 353/489 (72.19%), Postives = 422/489 (86.30%), Query Frame = 0

Query: 8   AAPDREERERQKKYWIEHSSDLTIESMMVDSDSSELDSEERAEVISLLPPYEGKTVAELG 67
           A P +EER+ QK YW+EHSSDLT+E+MM+DS +S+LD EER EV+SL+PPYEGK+V ELG
Sbjct: 2   ATPYKEERDIQKSYWMEHSSDLTVEAMMLDSKASDLDKEERPEVLSLIPPYEGKSVLELG 61

Query: 68  AGIGLFTAELAQRAAQVLAVDFIEEVIKKNEIINGHHKNVKFICADVTSPELDIAEESLD 127
           AGIG FT ELAQ+A +V+A+DFIE  I+KNE +NGH+KN+KF+CADVTSP+L I + S+D
Sbjct: 62  AGIGRFTGELAQKAGEVIALDFIESAIQKNESVNGHYKNIKFMCADVTSPDLKIKDGSID 121

Query: 128 LIFSNWLLMYLSDEEVKILAERMIKWLKVGGYIFFREACFQHSENFDKKHDLSHYREPRF 187
           LIFSNWLLMYLSD+EV+++AERMI W+K GGYIFFRE+CF  S +  +K + +HYREPRF
Sbjct: 122 LIFSNWLLMYLSDKEVELMAERMIGWVKPGGYIFFRESCFHQSGDSKRKSNPTHYREPRF 181

Query: 188 YSKVFKECYAKDDSGSVFELALLGCKPTGAYLRNKKSQNQICWIWQKVHSDDDRGFQQFL 247
           Y+KVF+EC  +D SG+ FEL+++GCK  GAY++NKK+QNQICWIWQKV  ++D+ FQ+FL
Sbjct: 182 YTKVFQECQTRDASGNSFELSMVGCKCIGAYVKNKKNQNQICWIWQKVSVENDKDFQRFL 241

Query: 248 DTVHYKSSGILRYERVFGHGFVSPGGLDTTKEFVAKLELKPGQKVLDVCCGIGGADFYMA 307
           D V YKSSGILRYERVFG G+VS GG +TTKEFVAK++LKPGQKVLDV CGIGG DFYMA
Sbjct: 242 DNVQYKSSGILRYERVFGEGYVSTGGFETTKEFVAKMDLKPGQKVLDVGCGIGGGDFYMA 301

Query: 308 ENFDVEVVGIDLSVNMISLALERAIGLKCSVEFEVADCTKKTYPDHTFDVIYSRDAILHI 367
           ENFDV VVGIDLSVNMIS ALERAIGLKCSVEFEVADCT KTYPD++FDVIYSRD ILHI
Sbjct: 302 ENFDVHVVGIDLSVNMISFALERAIGLKCSVEFEVADCTTKTYPDNSFDVIYSRDTILHI 361

Query: 368 QDKPALFRSFYKWLKPEGKLFITDYCRSAGTSSPEFAEYIKQGGYDLHDVKTYGQMIEDA 427
           QDKPALFR+F+KWLKP GK+ ITDYCRSA T SPEFAEYIKQ GYDLHDV+ YGQM++DA
Sbjct: 362 QDKPALFRTFFKWLKPGGKVLITDYCRSAETPSPEFAEYIKQRGYDLHDVQAYGQMLKDA 421

Query: 428 GFSEVVSEDRTYQFIQLLQRELEAVEEQKDTFIHDFSEEGYNHIVSGWKAKRNRGLSGEQ 487
           GF +V++EDRT QF+Q+L+RELE VE++K+ FI DFSEE YN IV GW AK  R  SGEQ
Sbjct: 422 GFDDVIAEDRTDQFVQVLRRELEKVEKEKEEFISDFSEEDYNDIVGGWSAKLERTASGEQ 481

Query: 488 RWGLFFAKK 497
           +WGLF A K
Sbjct: 482 KWGLFIADK 490

BLAST of Spg022328 vs. ExPASy Swiss-Prot
Match: Q9M571 (Phosphoethanolamine N-methyltransferase OS=Spinacia oleracea OX=3562 GN=PEAMT PE=1 SV=1)

HSP 1 Score: 739.2 bits (1907), Expect = 3.1e-212
Identity = 351/493 (71.20%), Postives = 426/493 (86.41%), Query Frame = 0

Query: 5   AESAAPDREERERQKKYWIEHSSDLTIESMMVDSDSSELDSEERAEVISLLPPYEGKTVA 64
           A SA    +ERE  KKYWIEHS DLT+E+MM+DS +S+LD  ER EV+S+LPPYEGK+V 
Sbjct: 2   AASAMGVLQEREVFKKYWIEHSVDLTVEAMMLDSQASDLDKVERPEVLSMLPPYEGKSVL 61

Query: 65  ELGAGIGLFTAELAQRAAQVLAVDFIEEVIKKNEIINGHHKNVKFICADVTSPELDIAEE 124
           ELGAGIG FT ELA++A+QV+A+DFIE VIKKNE INGH+KNVKF+CADVTSP L+I+  
Sbjct: 62  ELGAGIGRFTGELAEKASQVIALDFIESVIKKNESINGHYKNVKFMCADVTSPSLNISPN 121

Query: 125 SLDLIFSNWLLMYLSDEEVKILAERMIKWLKVGGYIFFREACFQHSENFDKKHDLSHYRE 184
           S+D+IFSNWLLMYLSDEEV+ L ERM+KWLK GGYIFFRE+CF  S +  +K + +HYRE
Sbjct: 122 SVDIIFSNWLLMYLSDEEVERLVERMLKWLKPGGYIFFRESCFHQSGDHKRKSNPTHYRE 181

Query: 185 PRFYSKVFKECYAKDDSGSVFELALLGCKPTGAYLRNKKSQNQICWIWQKVHSDDDRGFQ 244
           PRFY+K+FKEC+ +DDSG+ +EL+L+GCK  GAY+++KK+QNQI W+WQKV S+DD+GFQ
Sbjct: 182 PRFYTKIFKECHMQDDSGNSYELSLIGCKCIGAYVKSKKNQNQISWLWQKVDSEDDKGFQ 241

Query: 245 QFLDTVHYKSSGILRYERVFGHGFVSPGGLDTTKEFVAKLELKPGQKVLDVCCGIGGADF 304
           +FLD+  YK + ILRYERVFG G+VS GGL+TTKEFV+KL+LKPGQKVLDV CGIGG DF
Sbjct: 242 RFLDSSQYKFNSILRYERVFGPGYVSTGGLETTKEFVSKLDLKPGQKVLDVGCGIGGGDF 301

Query: 305 YMAENFDVEVVGIDLSVNMISLALERAIGLKCSVEFEVADCTKKTYPDHTFDVIYSRDAI 364
           YMAEN+DVEVVGIDLS+NMIS ALER+IGLKC+VEFEVADCTKK YP+++FDVIYSRD I
Sbjct: 302 YMAENYDVEVVGIDLSINMISFALERSIGLKCAVEFEVADCTKKDYPENSFDVIYSRDTI 361

Query: 365 LHIQDKPALFRSFYKWLKPEGKLFITDYCRSAGTSSPEFAEYIKQGGYDLHDVKTYGQMI 424
           LHIQDKPALFRSF+KWLKP GK+ I+DYC+SAGT S EFA YI+Q GYDLHDVK YG+M+
Sbjct: 362 LHIQDKPALFRSFHKWLKPGGKVLISDYCKSAGTPSAEFAAYIRQRGYDLHDVKAYGKML 421

Query: 425 EDAGFSEVVSEDRTYQFIQLLQRELEAVEEQKDTFIHDFSEEGYNHIVSGWKAKRNRGLS 484
           +DAGF EV++E+RT QFIQ+LQ+EL+A+E++KD FI DFSEE YN IV GWKAK  R   
Sbjct: 422 KDAGFVEVIAENRTDQFIQVLQKELDALEQEKDDFIDDFSEEDYNDIVDGWKAKLVRTTE 481

Query: 485 GEQRWGLFFAKKI 498
           GEQ+WGLF AKK+
Sbjct: 482 GEQQWGLFIAKKM 494

BLAST of Spg022328 vs. ExPASy Swiss-Prot
Match: Q9FR44 (Phosphoethanolamine N-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=NMT1 PE=1 SV=1)

HSP 1 Score: 738.8 bits (1906), Expect = 4.0e-212
Identity = 351/489 (71.78%), Postives = 420/489 (85.89%), Query Frame = 0

Query: 8   AAPDREERERQKKYWIEHSSDLTIESMMVDSDSSELDSEERAEVISLLPPYEGKTVAELG 67
           AA   EER+ QK YWIEHS+DLT+E+MM+DS +S+LD EER EV+SLLPPYEGK+V ELG
Sbjct: 2   AASYEEERDIQKNYWIEHSADLTVEAMMLDSRASDLDKEERPEVLSLLPPYEGKSVLELG 61

Query: 68  AGIGLFTAELAQRAAQVLAVDFIEEVIKKNEIINGHHKNVKFICADVTSPELDIAEESLD 127
           AGIG FT ELAQ+A +++A+DFI+ VIKKNE INGH+KNVKF+CADVTSP+L I + SLD
Sbjct: 62  AGIGRFTGELAQKAGELIALDFIDNVIKKNESINGHYKNVKFMCADVTSPDLKITDGSLD 121

Query: 128 LIFSNWLLMYLSDEEVKILAERMIKWLKVGGYIFFREACFQHSENFDKKHDLSHYREPRF 187
           LIFSNWLLMYLSD+EV++LAERM+ W+KVGGYIFFRE+CF  S +  +K + +HYREPRF
Sbjct: 122 LIFSNWLLMYLSDKEVELLAERMVGWIKVGGYIFFRESCFHQSGDSKRKSNPTHYREPRF 181

Query: 188 YSKVFKECYAKDDSGSVFELALLGCKPTGAYLRNKKSQNQICWIWQKVHSDDDRGFQQFL 247
           YSKVF+EC  +D +G+ FEL+++GCK  GAY++NKK+QNQICWIWQKV S++DRGFQ+FL
Sbjct: 182 YSKVFQECQTRDAAGNSFELSMIGCKCIGAYVKNKKNQNQICWIWQKVSSENDRGFQRFL 241

Query: 248 DTVHYKSSGILRYERVFGHGFVSPGGLDTTKEFVAKLELKPGQKVLDVCCGIGGADFYMA 307
           D V YKSSGILRYERVFG GFVS GGL+TTKEFV K+ LKPGQKVLDV CGIGG DFYMA
Sbjct: 242 DNVQYKSSGILRYERVFGQGFVSTGGLETTKEFVEKMNLKPGQKVLDVGCGIGGGDFYMA 301

Query: 308 ENFDVEVVGIDLSVNMISLALERAIGLKCSVEFEVADCTKKTYPDHTFDVIYSRDAILHI 367
           E FDV VVGIDLSVNMIS ALERAIGL CSVEFEVADCT K YPD++FDVIYSRD ILHI
Sbjct: 302 EKFDVHVVGIDLSVNMISFALERAIGLSCSVEFEVADCTTKHYPDNSFDVIYSRDTILHI 361

Query: 368 QDKPALFRSFYKWLKPEGKLFITDYCRSAGTSSPEFAEYIKQGGYDLHDVKTYGQMIEDA 427
           QDKPALFR+F+KWLKP GK+ I+DYCRS  T S EF+EYIKQ GYDLHDV+ YGQM++DA
Sbjct: 362 QDKPALFRTFFKWLKPGGKVLISDYCRSPKTPSAEFSEYIKQRGYDLHDVQAYGQMLKDA 421

Query: 428 GFSEVVSEDRTYQFIQLLQRELEAVEEQKDTFIHDFSEEGYNHIVSGWKAKRNRGLSGEQ 487
           GF++V++EDRT QF+Q+L+REL+ VE++K+ FI DFS+E Y+ IV GWK+K  R  S EQ
Sbjct: 422 GFTDVIAEDRTDQFMQVLKRELDRVEKEKEKFISDFSKEDYDDIVGGWKSKLERCASDEQ 481

Query: 488 RWGLFFAKK 497
           +WGLF A K
Sbjct: 482 KWGLFIANK 490

BLAST of Spg022328 vs. ExPASy Swiss-Prot
Match: Q9C6B9 (Phosphoethanolamine N-methyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=NMT3 PE=2 SV=2)

HSP 1 Score: 729.9 bits (1883), Expect = 1.9e-209
Identity = 345/484 (71.28%), Postives = 411/484 (84.92%), Query Frame = 0

Query: 13  EERERQKKYWIEHSSDLTIESMMVDSDSSELDSEERAEVISLLPPYEGKTVAELGAGIGL 72
           EERE QK YW EHS  L++E+MM+DS +S+LD EER E+++ LPP EG TV E GAGIG 
Sbjct: 6   EEREIQKNYWKEHSVGLSVEAMMLDSKASDLDKEERPEILAFLPPIEGTTVLEFGAGIGR 65

Query: 73  FTAELAQRAAQVLAVDFIEEVIKKNEIINGHHKNVKFICADVTSPELDIAEESLDLIFSN 132
           FT ELAQ+A QV+AVDFIE VIKKNE INGH+KNVKF+CADVTSP ++   ES+DLIFSN
Sbjct: 66  FTTELAQKAGQVIAVDFIESVIKKNENINGHYKNVKFLCADVTSPNMNFPNESMDLIFSN 125

Query: 133 WLLMYLSDEEVKILAERMIKWLKVGGYIFFREACFQHSENFDKKHDLSHYREPRFYSKVF 192
           WLLMYLSD+EV+ LA++M++W KVGGYIFFRE+CF  S +  +K++ +HYREP+FY+K+F
Sbjct: 126 WLLMYLSDQEVEDLAKKMLQWTKVGGYIFFRESCFHQSGDNKRKYNPTHYREPKFYTKLF 185

Query: 193 KECYAKDDSGSVFELALLGCKPTGAYLRNKKSQNQICWIWQKVHSDDDRGFQQFLDTVHY 252
           KEC+  D+ G+ +EL+L+ CK  GAY+RNKK+QNQICW+WQKV SD+DRGFQ+FLD V Y
Sbjct: 186 KECHMNDEDGNSYELSLVSCKCIGAYVRNKKNQNQICWLWQKVSSDNDRGFQRFLDNVQY 245

Query: 253 KSSGILRYERVFGHGFVSPGGLDTTKEFVAKLELKPGQKVLDVCCGIGGADFYMAENFDV 312
           KSSGILRYERVFG GFVS GGL+TTKEFV  L+LKPGQKVLDV CGIGG DFYMAENFDV
Sbjct: 246 KSSGILRYERVFGEGFVSTGGLETTKEFVDMLDLKPGQKVLDVGCGIGGGDFYMAENFDV 305

Query: 313 EVVGIDLSVNMISLALERAIGLKCSVEFEVADCTKKTYPDHTFDVIYSRDAILHIQDKPA 372
           +VVGIDLSVNMIS ALE AIGLKCSVEFEVADCTKK YPD+TFDVIYSRD ILHIQDKPA
Sbjct: 306 DVVGIDLSVNMISFALEHAIGLKCSVEFEVADCTKKEYPDNTFDVIYSRDTILHIQDKPA 365

Query: 373 LFRSFYKWLKPEGKLFITDYCRSAGTSSPEFAEYIKQGGYDLHDVKTYGQMIEDAGFSEV 432
           LFR FYKWLKP GK+ ITDYCRS  T SP+FA YIK+ GYDLHDV+ YGQM+ DAGF EV
Sbjct: 366 LFRRFYKWLKPGGKVLITDYCRSPKTPSPDFAIYIKKRGYDLHDVQAYGQMLRDAGFEEV 425

Query: 433 VSEDRTYQFIQLLQRELEAVEEQKDTFIHDFSEEGYNHIVSGWKAKRNRGLSGEQRWGLF 492
           ++EDRT QF+++L+REL+AVE++K+ FI DFS+E Y  I+ GWK+K  R  SGEQ+WGLF
Sbjct: 426 IAEDRTDQFMKVLKRELDAVEKEKEEFISDFSKEDYEDIIGGWKSKLLRSSSGEQKWGLF 485

Query: 493 FAKK 497
            AK+
Sbjct: 486 IAKR 489

BLAST of Spg022328 vs. ExPASy Swiss-Prot
Match: Q22993 (Phosphoethanolamine N-methyltransferase 2 OS=Caenorhabditis elegans OX=6239 GN=pmt-2 PE=1 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 1.4e-42
Identity = 95/256 (37.11%), Postives = 141/256 (55.08%), Query Frame = 0

Query: 243 FQQFLDTVHYKSSGILRYERVFGHGFVSPGGLDTTKEFVAKL-ELKPGQKVLDVCCGIGG 302
           F+ FLD   Y ++GI  YE +FG  F+SPGG D   + + +  + KPGQ +LD+  GIGG
Sbjct: 178 FRDFLDKTQYTNTGIDAYEWMFGVNFISPGGYDENLKIIKRFGDFKPGQTMLDIGVGIGG 237

Query: 303 ADFYMAENFDVEVVGIDLSVNMISLALERAIGLKCS-VEFEVADCTKKTYPDHTFDVIYS 362
               +A+ F V V GIDLS NM+++ALER    K S V++ + D     + D++FD ++S
Sbjct: 238 GARQVADEFGVHVHGIDLSSNMLAIALERLHEEKDSRVKYSITDALVYQFEDNSFDYVFS 297

Query: 363 RDAILHIQDKPALFRSFYKWLKPEGKLFITDYCRSAGTSSPEFAEYIKQGGYDLHDVKTY 422
           RD I HI D   LF   YK LKP GK+ IT Y +  G  S +F  Y+ Q  Y L ++K  
Sbjct: 298 RDCIQHIPDTEKLFSRIYKALKPGGKVLITMYGKGYGEQSDKFKTYVAQRAYFLKNLKEI 357

Query: 423 GQMIEDAGFSEVVSEDRTYQFIQLLQRELEAVEEQKDTFIHDFSEEGYNHIVSGWKAKRN 482
             +    GF  V +E+ T +F ++L  E   +E+ +  F+  F++   + ++SGW  K  
Sbjct: 358 ADIANKTGFVNVQTENMTPRFKEILLEERGHLEQNEAEFMSKFTQRERDSLISGWTDKLG 417

Query: 483 RGLSGEQRWGLFFAKK 497
                   W  F A+K
Sbjct: 418 YIEKDNHNWNFFLAQK 433

BLAST of Spg022328 vs. ExPASy TrEMBL
Match: A0A5A7UVD8 (Phosphomethylethanolamine N-methyltransferase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G001250 PE=4 SV=1)

HSP 1 Score: 850.1 bits (2195), Expect = 4.6e-243
Identity = 420/499 (84.17%), Postives = 456/499 (91.38%), Query Frame = 0

Query: 1   MASSAESAAPDREERERQKKYWIEHSSDLTIESMMVDSDSSELDSEERAEVISLLPPYEG 60
           MAS  +S   D  +R+RQKKYWIEHSSDLT+ES+MVDSDS ELDSEE+AEVISLLPPYEG
Sbjct: 1   MASFVKSTFSD--QRDRQKKYWIEHSSDLTMESVMVDSDSHELDSEEKAEVISLLPPYEG 60

Query: 61  KTVAELGAGIGLFTAELAQRAAQVLAVDFIEEVIKKNEIINGHHKNVKFICADVTSPELD 120
           KTV ELGAG+G FTAELAQRAAQV+AVDF+E+VI+KNE INGHHKNVKF+ ADVTSPELD
Sbjct: 61  KTVVELGAGVGRFTAELAQRAAQVIAVDFVEDVIRKNESINGHHKNVKFLWADVTSPELD 120

Query: 121 IAEESLDLIFSNWLLMYLSDEEVKILAERMIKWLKVGGYIFFREACFQHSENFDKKHDLS 180
           I E+S+D IFSN LLMYLSDEEVK+LAERMIKWLKVGGYIFFRE+CFQ SEN DKKHD+S
Sbjct: 121 IHEDSVDFIFSNLLLMYLSDEEVKLLAERMIKWLKVGGYIFFRESCFQDSENSDKKHDVS 180

Query: 181 HYREPRFYSKVFKE-CYAKDDSGSVFELALLGCKPTGAYLRNKKSQNQICWIWQKVHSDD 240
           ++REPRFYSKVFKE C+ KDDS + FEL+LL CKPTG +  NKKS+NQICWIWQKV SD+
Sbjct: 181 YHREPRFYSKVFKELCFVKDDSENAFELSLLDCKPTGVF-GNKKSRNQICWIWQKVRSDN 240

Query: 241 DRGFQQFLDTVHYKSSGILRYERVFGHGFVSPGGLDTTKEFVAKLELKPGQKVLDVCCGI 300
           DRGFQQFLDTV YKSSGIL+YERVFGHGF+SPGGL+TTKEFVAKL+LKP Q+VLDV CGI
Sbjct: 241 DRGFQQFLDTVQYKSSGILKYERVFGHGFISPGGLETTKEFVAKLDLKPRQRVLDVGCGI 300

Query: 301 GGADFYMAENFDVEVVGIDLSVNMISLALERAIGLKCSVEFEVADCTKKTYPDHTFDVIY 360
           GGADFYM ENF VEVVGIDLSVNMISLALERAIGL CSVEFEVADCTKKTYPDH+FDVIY
Sbjct: 301 GGADFYMVENFSVEVVGIDLSVNMISLALERAIGLSCSVEFEVADCTKKTYPDHSFDVIY 360

Query: 361 SRDAILHIQDKPALFRSFYKWLKPEGKLFITDYCRSAGTSSPEFAEYIKQGGYDLHDVKT 420
           SRD ILHIQDKP+LFRSFYKWLKP GKLFITDYC++AGTSSPEFAEYIKQ GYDLHDVKT
Sbjct: 361 SRDTILHIQDKPSLFRSFYKWLKPGGKLFITDYCKNAGTSSPEFAEYIKQRGYDLHDVKT 420

Query: 421 YGQMIEDAGFSEVVSEDRTYQFIQLLQRELEAVEEQKDTFIHDFSEEGYNHIVSGWKAKR 480
           YGQMIEDAGF EVVSEDRT QFIQ+LQRELEAVEEQKD FI DFSEEGYN I+ GWKAK 
Sbjct: 421 YGQMIEDAGFGEVVSEDRTNQFIQVLQRELEAVEEQKDRFILDFSEEGYNDIICGWKAKL 480

Query: 481 NRGLSGEQRWGLFFAKKIN 499
           NR LSGEQRWGLFFAKKIN
Sbjct: 481 NRSLSGEQRWGLFFAKKIN 496

BLAST of Spg022328 vs. ExPASy TrEMBL
Match: A0A0A0LPY1 (Methyltransf_25 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G025050 PE=4 SV=1)

HSP 1 Score: 815.5 bits (2105), Expect = 1.3e-232
Identity = 403/469 (85.93%), Postives = 431/469 (91.90%), Query Frame = 0

Query: 31  IESMMVDSDSSELDSEERAEVISLLPPYEGKTVAELGAGIGLFTAELAQRAAQVLAVDFI 90
           +ESMMVDSDS ELDSEE AEVISLLPPYEGKTV ELGAGIG FT+ELAQRAAQV+AVDF+
Sbjct: 1   MESMMVDSDSHELDSEETAEVISLLPPYEGKTVVELGAGIGRFTSELAQRAAQVIAVDFV 60

Query: 91  EEVIKKNEIINGHHKNVKFICADVTSPELDIAEESLDLIFSNWLLMYLSDEEVKILAERM 150
           E+VI+KNE INGHHKNVKF CADVT  ELDI E+S+D IFSN LLMYLSDEEVK+LAERM
Sbjct: 61  EDVIRKNESINGHHKNVKFQCADVTFSELDIHEDSVDFIFSNLLLMYLSDEEVKLLAERM 120

Query: 151 IKWLKVGGYIFFREACFQHSENFDKKHDLSHYREPRFYSKVFKE-CYAKDDSGSVFELAL 210
           IKWLKVGGYIFFRE+CFQH EN DKK+D+S++REPRFYSKVFKE C+ KDDS +VFEL+L
Sbjct: 121 IKWLKVGGYIFFRESCFQHYENSDKKNDVSYHREPRFYSKVFKELCFVKDDSENVFELSL 180

Query: 211 LGCKPTGAYLRNKKSQNQICWIWQKVHSDDDRGFQQFLDTVHYKSSGILRYERVFGHGFV 270
           LGCKPTG +  NKKSQNQICWIWQKV SD+DRGFQ FLDTV YKSSGIL+YERVFGHGF+
Sbjct: 181 LGCKPTGVF-GNKKSQNQICWIWQKVRSDNDRGFQLFLDTVQYKSSGILKYERVFGHGFI 240

Query: 271 SPGGLDTTKEFVAKLELKPGQKVLDVCCGIGGADFYMAENFDVEVVGIDLSVNMISLALE 330
           SPGGL+TTKEFVAKL+LKP Q+VLDV CGIGGADFYMAENF VEVVGIDLSVNMISLALE
Sbjct: 241 SPGGLETTKEFVAKLDLKPRQRVLDVGCGIGGADFYMAENFSVEVVGIDLSVNMISLALE 300

Query: 331 RAIGLKCSVEFEVADCTKKTYPDHTFDVIYSRDAILHIQDKPALFRSFYKWLKPEGKLFI 390
           RAIGL CSVEFEVADCTKKTYPDHTFDVIYSRD ILHIQDKP+LFRSFYKWLKP GKLFI
Sbjct: 301 RAIGLACSVEFEVADCTKKTYPDHTFDVIYSRDTILHIQDKPSLFRSFYKWLKPGGKLFI 360

Query: 391 TDYCRSAGTSSPEFAEYIKQGGYDLHDVKTYGQMIEDAGFSEVVSEDRTYQFIQLLQREL 450
           TDYCR+AGTSSPEFAEYIKQ GYDLHDVK YGQMIEDAGF EVVSEDRT QFIQ+LQ+EL
Sbjct: 361 TDYCRNAGTSSPEFAEYIKQRGYDLHDVKAYGQMIEDAGFGEVVSEDRTNQFIQVLQQEL 420

Query: 451 EAVEEQKDTFIHDFSEEGYNHIVSGWKAKRNRGLSGEQRWGLFFAKKIN 499
           EAVEEQKD FI DFSEEGYN IV GWKAK NR LSGEQRWGLFFAKKIN
Sbjct: 421 EAVEEQKDRFILDFSEEGYNDIVCGWKAKLNRSLSGEQRWGLFFAKKIN 468

BLAST of Spg022328 vs. ExPASy TrEMBL
Match: B9T1H8 (Phosphoethanolamine n-methyltransferase, putative OS=Ricinus communis OX=3988 GN=RCOM_0161380 PE=4 SV=1)

HSP 1 Score: 780.0 bits (2013), Expect = 5.8e-222
Identity = 374/490 (76.33%), Postives = 428/490 (87.35%), Query Frame = 0

Query: 7   SAAPDREERERQKKYWIEHSSDLTIESMMVDSDSSELDSEERAEVISLLPPYEGKTVAEL 66
           +AA + EERE QK YWIEHS DLT+E+MM+DS +S+LD EER EV+SLLP YEGK+V E 
Sbjct: 2   AAAQNVEEREVQKNYWIEHSVDLTVEAMMLDSKASDLDKEERPEVLSLLPSYEGKSVLEF 61

Query: 67  GAGIGLFTAELAQRAAQVLAVDFIEEVIKKNEIINGHHKNVKFICADVTSPELDIAEESL 126
           GAGIG FT ELAQ+A Q++AVDFIE VIKKNE INGHHKNVKF+CADVTS  L  +EES+
Sbjct: 62  GAGIGRFTGELAQKAGQLVAVDFIESVIKKNENINGHHKNVKFMCADVTSQGLKFSEESV 121

Query: 127 DLIFSNWLLMYLSDEEVKILAERMIKWLKVGGYIFFREACFQHSENFDKKHDLSHYREPR 186
           DLIFSNWLLMYLSDEEV  LAERM+KWLKVGGYIFFRE+CF  S +  +KH+ +HYREPR
Sbjct: 122 DLIFSNWLLMYLSDEEVVNLAERMVKWLKVGGYIFFRESCFHQSGDSKRKHNPTHYREPR 181

Query: 187 FYSKVFKECYAKDDSGSVFELALLGCKPTGAYLRNKKSQNQICWIWQKVHSDDDRGFQQF 246
           FY+KVFKEC+A D SG+ FEL+L+GCK  GAY+RNKK+QNQICW+WQKV S DD+GFQ+F
Sbjct: 182 FYTKVFKECHASDGSGNSFELSLIGCKCIGAYVRNKKNQNQICWVWQKVSSQDDKGFQRF 241

Query: 247 LDTVHYKSSGILRYERVFGHGFVSPGGLDTTKEFVAKLELKPGQKVLDVCCGIGGADFYM 306
           LD V YKS+GILRYERVFG GFVS GG++TTKEFVAKL+LKPGQKVLDV CGIGG DFYM
Sbjct: 242 LDNVQYKSNGILRYERVFGPGFVSTGGVETTKEFVAKLDLKPGQKVLDVGCGIGGGDFYM 301

Query: 307 AENFDVEVVGIDLSVNMISLALERAIGLKCSVEFEVADCTKKTYPDHTFDVIYSRDAILH 366
           AENFDVEV+GIDLS+NMIS ALERAIGLKC+VEFEVADCTKKTYPD++FDVIYSRD ILH
Sbjct: 302 AENFDVEVIGIDLSINMISFALERAIGLKCAVEFEVADCTKKTYPDNSFDVIYSRDTILH 361

Query: 367 IQDKPALFRSFYKWLKPEGKLFITDYCRSAGTSSPEFAEYIKQGGYDLHDVKTYGQMIED 426
           IQDKPALFRSFYKWLKP GK+ I+DYC+SAGT S EFAEYIKQ GYDLHDVK YGQM++D
Sbjct: 362 IQDKPALFRSFYKWLKPGGKVLISDYCKSAGTPSSEFAEYIKQRGYDLHDVKAYGQMLKD 421

Query: 427 AGFSEVVSEDRTYQFIQLLQRELEAVEEQKDTFIHDFSEEGYNHIVSGWKAKRNRGLSGE 486
           AGF +V++EDRT QF Q+L+REL+ +E++KD FIHDFSEE YN IV GWKAK  R  SGE
Sbjct: 422 AGFDDVIAEDRTDQFNQVLKRELDVIEKEKDEFIHDFSEEDYNDIVGGWKAKLIRSSSGE 481

Query: 487 QRWGLFFAKK 497
           QRWGLF AKK
Sbjct: 482 QRWGLFIAKK 491

BLAST of Spg022328 vs. ExPASy TrEMBL
Match: A0A2I4HPB0 (phosphomethylethanolamine N-methyltransferase OS=Juglans regia OX=51240 GN=LOC109020045 PE=4 SV=1)

HSP 1 Score: 778.9 bits (2010), Expect = 1.3e-221
Identity = 374/484 (77.27%), Postives = 424/484 (87.60%), Query Frame = 0

Query: 13  EERERQKKYWIEHSSDLTIESMMVDSDSSELDSEERAEVISLLPPYEGKTVAELGAGIGL 72
           +ERE QK YWIE+S+DL++E+MM+DS +S+LD EER EV+SLLPPYEGKTV ELGAGIG 
Sbjct: 7   QEREVQKCYWIENSADLSVEAMMLDSKASDLDKEERPEVLSLLPPYEGKTVLELGAGIGR 66

Query: 73  FTAELAQRAAQVLAVDFIEEVIKKNEIINGHHKNVKFICADVTSPELDIAEESLDLIFSN 132
           FT ELAQ+A ++LA+DFIE VIKKNE INGHHKNVKF+CADVTSP+L I++ES+D IFSN
Sbjct: 67  FTGELAQKAGELLALDFIESVIKKNESINGHHKNVKFMCADVTSPDLKISDESVDFIFSN 126

Query: 133 WLLMYLSDEEVKILAERMIKWLKVGGYIFFREACFQHSENFDKKHDLSHYREPRFYSKVF 192
           WLLMYLSD+EV+ LAERM+KWLKVGG++FFRE+CF  S +  +K++ +HYREPRFY+K+F
Sbjct: 127 WLLMYLSDKEVEDLAERMVKWLKVGGFLFFRESCFHQSGDSKRKYNPTHYREPRFYTKLF 186

Query: 193 KECYAKDDSGSVFELALLGCKPTGAYLRNKKSQNQICWIWQKVHSDDDRGFQQFLDTVHY 252
           KEC+  D SG+ FELAL GCK  GAY+RNKK+QNQICW WQKV S DDRGFQQFLD V Y
Sbjct: 187 KECHMLDGSGNSFELALAGCKCIGAYVRNKKNQNQICWTWQKVSSQDDRGFQQFLDNVQY 246

Query: 253 KSSGILRYERVFGHGFVSPGGLDTTKEFVAKLELKPGQKVLDVCCGIGGADFYMAENFDV 312
           KSSGILRYERVFG GFVS GG+DTTKEFV KLELKPGQKVLDV CGIGG DFYMAENFDV
Sbjct: 247 KSSGILRYERVFGPGFVSTGGIDTTKEFVQKLELKPGQKVLDVGCGIGGGDFYMAENFDV 306

Query: 313 EVVGIDLSVNMISLALERAIGLKCSVEFEVADCTKKTYPDHTFDVIYSRDAILHIQDKPA 372
           EVVGIDLS+NM+S ALERAIGLKC VEFEVADCTKKTYPD+TFDVIYSRD ILHIQDKPA
Sbjct: 307 EVVGIDLSINMVSFALERAIGLKCPVEFEVADCTKKTYPDNTFDVIYSRDTILHIQDKPA 366

Query: 373 LFRSFYKWLKPEGKLFITDYCRSAGTSSPEFAEYIKQGGYDLHDVKTYGQMIEDAGFSEV 432
           LFRSFYKWLKP GK+ I+DYCR+AGT SPEF+EYIKQ GYDLHDVK YGQM+ DAGF EV
Sbjct: 367 LFRSFYKWLKPGGKVLISDYCRNAGTPSPEFSEYIKQRGYDLHDVKAYGQMLRDAGFGEV 426

Query: 433 VSEDRTYQFIQLLQRELEAVEEQKDTFIHDFSEEGYNHIVSGWKAKRNRGLSGEQRWGLF 492
           V+EDRT QFIQ+LQREL A+E+ KD FI DFSEE YN IV GWKAK  R  SGEQRWGLF
Sbjct: 427 VAEDRTDQFIQVLQRELNAIEKDKDEFICDFSEEDYNDIVGGWKAKLVRSSSGEQRWGLF 486

Query: 493 FAKK 497
            A+K
Sbjct: 487 IARK 490

BLAST of Spg022328 vs. ExPASy TrEMBL
Match: A0A5N6RTY7 (Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_019662 PE=4 SV=1)

HSP 1 Score: 773.9 bits (1997), Expect = 4.2e-220
Identity = 371/484 (76.65%), Postives = 424/484 (87.60%), Query Frame = 0

Query: 13  EERERQKKYWIEHSSDLTIESMMVDSDSSELDSEERAEVISLLPPYEGKTVAELGAGIGL 72
           EERE QK YWIEHS+DLT+E+MM+DS +S+LD EER EV+S+LP YEGK+V ELGAGIG 
Sbjct: 7   EEREMQKNYWIEHSADLTVEAMMLDSKASDLDKEERPEVLSILPSYEGKSVLELGAGIGR 66

Query: 73  FTAELAQRAAQVLAVDFIEEVIKKNEIINGHHKNVKFICADVTSPELDIAEESLDLIFSN 132
           FT ELAQ+A Q+LA+DFIE VIKKNE INGH+KNVK +CADVTSP+L I+EES+DLIFSN
Sbjct: 67  FTGELAQKAGQLLALDFIESVIKKNESINGHYKNVKLMCADVTSPDLKISEESMDLIFSN 126

Query: 133 WLLMYLSDEEVKILAERMIKWLKVGGYIFFREACFQHSENFDKKHDLSHYREPRFYSKVF 192
           WLLMYLSDEEV+ +AERMIKWLKVGG+IFFRE+CF  S +  +K++ +HYREPRFY+KVF
Sbjct: 127 WLLMYLSDEEVEYVAERMIKWLKVGGFIFFRESCFHQSGDSKRKYNPTHYREPRFYTKVF 186

Query: 193 KECYAKDDSGSVFELALLGCKPTGAYLRNKKSQNQICWIWQKVHSDDDRGFQQFLDTVHY 252
           KEC+  D SG+ FEL+L+GCK  GAY+RNKK+QNQICWIWQKV S DDRGFQ+FLDTV Y
Sbjct: 187 KECHLSDGSGNSFELSLVGCKCIGAYVRNKKNQNQICWIWQKVSSQDDRGFQRFLDTVQY 246

Query: 253 KSSGILRYERVFGHGFVSPGGLDTTKEFVAKLELKPGQKVLDVCCGIGGADFYMAENFDV 312
           KS+GILRYERVFG GFVS GG+DTTKEFV KLELKPGQKVLDV CGIGG DFYMAENFDV
Sbjct: 247 KSNGILRYERVFGQGFVSTGGIDTTKEFVQKLELKPGQKVLDVGCGIGGGDFYMAENFDV 306

Query: 313 EVVGIDLSVNMISLALERAIGLKCSVEFEVADCTKKTYPDHTFDVIYSRDAILHIQDKPA 372
           EVVGIDLS+NMIS ALERAIGLKCSVEFEVADCTKKTYP++ FDVIYSRD ILHIQDKPA
Sbjct: 307 EVVGIDLSINMISFALERAIGLKCSVEFEVADCTKKTYPENAFDVIYSRDTILHIQDKPA 366

Query: 373 LFRSFYKWLKPEGKLFITDYCRSAGTSSPEFAEYIKQGGYDLHDVKTYGQMIEDAGFSEV 432
           LFRSFYKWLKP GK+ I+DYC+SAGT SPEF+EYIKQ GYDLHDVK YGQM++DAGF EV
Sbjct: 367 LFRSFYKWLKPGGKVLISDYCKSAGTPSPEFSEYIKQRGYDLHDVKAYGQMLKDAGFDEV 426

Query: 433 VSEDRTYQFIQLLQRELEAVEEQKDTFIHDFSEEGYNHIVSGWKAKRNRGLSGEQRWGLF 492
           ++ED T QF+Q+LQREL A+ + KD FI DFS E Y+ IV GWKAK  R  SGEQRWGLF
Sbjct: 427 IAEDCTDQFMQVLQRELNALAKDKDEFIRDFSGEDYSDIVGGWKAKLIRSSSGEQRWGLF 486

Query: 493 FAKK 497
            A+K
Sbjct: 487 VARK 490

BLAST of Spg022328 vs. TAIR 10
Match: AT1G48600.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 750.0 bits (1935), Expect = 1.2e-216
Identity = 353/489 (72.19%), Postives = 422/489 (86.30%), Query Frame = 0

Query: 8   AAPDREERERQKKYWIEHSSDLTIESMMVDSDSSELDSEERAEVISLLPPYEGKTVAELG 67
           A P +EER+ QK YW+EHSSDLT+E+MM+DS +S+LD EER EV+SL+PPYEGK+V ELG
Sbjct: 2   ATPYKEERDIQKSYWMEHSSDLTVEAMMLDSKASDLDKEERPEVLSLIPPYEGKSVLELG 61

Query: 68  AGIGLFTAELAQRAAQVLAVDFIEEVIKKNEIINGHHKNVKFICADVTSPELDIAEESLD 127
           AGIG FT ELAQ+A +V+A+DFIE  I+KNE +NGH+KN+KF+CADVTSP+L I + S+D
Sbjct: 62  AGIGRFTGELAQKAGEVIALDFIESAIQKNESVNGHYKNIKFMCADVTSPDLKIKDGSID 121

Query: 128 LIFSNWLLMYLSDEEVKILAERMIKWLKVGGYIFFREACFQHSENFDKKHDLSHYREPRF 187
           LIFSNWLLMYLSD+EV+++AERMI W+K GGYIFFRE+CF  S +  +K + +HYREPRF
Sbjct: 122 LIFSNWLLMYLSDKEVELMAERMIGWVKPGGYIFFRESCFHQSGDSKRKSNPTHYREPRF 181

Query: 188 YSKVFKECYAKDDSGSVFELALLGCKPTGAYLRNKKSQNQICWIWQKVHSDDDRGFQQFL 247
           Y+KVF+EC  +D SG+ FEL+++GCK  GAY++NKK+QNQICWIWQKV  ++D+ FQ+FL
Sbjct: 182 YTKVFQECQTRDASGNSFELSMVGCKCIGAYVKNKKNQNQICWIWQKVSVENDKDFQRFL 241

Query: 248 DTVHYKSSGILRYERVFGHGFVSPGGLDTTKEFVAKLELKPGQKVLDVCCGIGGADFYMA 307
           D V YKSSGILRYERVFG G+VS GG +TTKEFVAK++LKPGQKVLDV CGIGG DFYMA
Sbjct: 242 DNVQYKSSGILRYERVFGEGYVSTGGFETTKEFVAKMDLKPGQKVLDVGCGIGGGDFYMA 301

Query: 308 ENFDVEVVGIDLSVNMISLALERAIGLKCSVEFEVADCTKKTYPDHTFDVIYSRDAILHI 367
           ENFDV VVGIDLSVNMIS ALERAIGLKCSVEFEVADCT KTYPD++FDVIYSRD ILHI
Sbjct: 302 ENFDVHVVGIDLSVNMISFALERAIGLKCSVEFEVADCTTKTYPDNSFDVIYSRDTILHI 361

Query: 368 QDKPALFRSFYKWLKPEGKLFITDYCRSAGTSSPEFAEYIKQGGYDLHDVKTYGQMIEDA 427
           QDKPALFR+F+KWLKP GK+ ITDYCRSA T SPEFAEYIKQ GYDLHDV+ YGQM++DA
Sbjct: 362 QDKPALFRTFFKWLKPGGKVLITDYCRSAETPSPEFAEYIKQRGYDLHDVQAYGQMLKDA 421

Query: 428 GFSEVVSEDRTYQFIQLLQRELEAVEEQKDTFIHDFSEEGYNHIVSGWKAKRNRGLSGEQ 487
           GF +V++EDRT QF+Q+L+RELE VE++K+ FI DFSEE YN IV GW AK  R  SGEQ
Sbjct: 422 GFDDVIAEDRTDQFVQVLRRELEKVEKEKEEFISDFSEEDYNDIVGGWSAKLERTASGEQ 481

Query: 488 RWGLFFAKK 497
           +WGLF A K
Sbjct: 482 KWGLFIADK 490

BLAST of Spg022328 vs. TAIR 10
Match: AT3G18000.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 738.8 bits (1906), Expect = 2.9e-213
Identity = 351/489 (71.78%), Postives = 420/489 (85.89%), Query Frame = 0

Query: 8   AAPDREERERQKKYWIEHSSDLTIESMMVDSDSSELDSEERAEVISLLPPYEGKTVAELG 67
           AA   EER+ QK YWIEHS+DLT+E+MM+DS +S+LD EER EV+SLLPPYEGK+V ELG
Sbjct: 2   AASYEEERDIQKNYWIEHSADLTVEAMMLDSRASDLDKEERPEVLSLLPPYEGKSVLELG 61

Query: 68  AGIGLFTAELAQRAAQVLAVDFIEEVIKKNEIINGHHKNVKFICADVTSPELDIAEESLD 127
           AGIG FT ELAQ+A +++A+DFI+ VIKKNE INGH+KNVKF+CADVTSP+L I + SLD
Sbjct: 62  AGIGRFTGELAQKAGELIALDFIDNVIKKNESINGHYKNVKFMCADVTSPDLKITDGSLD 121

Query: 128 LIFSNWLLMYLSDEEVKILAERMIKWLKVGGYIFFREACFQHSENFDKKHDLSHYREPRF 187
           LIFSNWLLMYLSD+EV++LAERM+ W+KVGGYIFFRE+CF  S +  +K + +HYREPRF
Sbjct: 122 LIFSNWLLMYLSDKEVELLAERMVGWIKVGGYIFFRESCFHQSGDSKRKSNPTHYREPRF 181

Query: 188 YSKVFKECYAKDDSGSVFELALLGCKPTGAYLRNKKSQNQICWIWQKVHSDDDRGFQQFL 247
           YSKVF+EC  +D +G+ FEL+++GCK  GAY++NKK+QNQICWIWQKV S++DRGFQ+FL
Sbjct: 182 YSKVFQECQTRDAAGNSFELSMIGCKCIGAYVKNKKNQNQICWIWQKVSSENDRGFQRFL 241

Query: 248 DTVHYKSSGILRYERVFGHGFVSPGGLDTTKEFVAKLELKPGQKVLDVCCGIGGADFYMA 307
           D V YKSSGILRYERVFG GFVS GGL+TTKEFV K+ LKPGQKVLDV CGIGG DFYMA
Sbjct: 242 DNVQYKSSGILRYERVFGQGFVSTGGLETTKEFVEKMNLKPGQKVLDVGCGIGGGDFYMA 301

Query: 308 ENFDVEVVGIDLSVNMISLALERAIGLKCSVEFEVADCTKKTYPDHTFDVIYSRDAILHI 367
           E FDV VVGIDLSVNMIS ALERAIGL CSVEFEVADCT K YPD++FDVIYSRD ILHI
Sbjct: 302 EKFDVHVVGIDLSVNMISFALERAIGLSCSVEFEVADCTTKHYPDNSFDVIYSRDTILHI 361

Query: 368 QDKPALFRSFYKWLKPEGKLFITDYCRSAGTSSPEFAEYIKQGGYDLHDVKTYGQMIEDA 427
           QDKPALFR+F+KWLKP GK+ I+DYCRS  T S EF+EYIKQ GYDLHDV+ YGQM++DA
Sbjct: 362 QDKPALFRTFFKWLKPGGKVLISDYCRSPKTPSAEFSEYIKQRGYDLHDVQAYGQMLKDA 421

Query: 428 GFSEVVSEDRTYQFIQLLQRELEAVEEQKDTFIHDFSEEGYNHIVSGWKAKRNRGLSGEQ 487
           GF++V++EDRT QF+Q+L+REL+ VE++K+ FI DFS+E Y+ IV GWK+K  R  S EQ
Sbjct: 422 GFTDVIAEDRTDQFMQVLKRELDRVEKEKEKFISDFSKEDYDDIVGGWKSKLERCASDEQ 481

Query: 488 RWGLFFAKK 497
           +WGLF A K
Sbjct: 482 KWGLFIANK 490

BLAST of Spg022328 vs. TAIR 10
Match: AT1G48600.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 731.5 bits (1887), Expect = 4.6e-211
Identity = 344/474 (72.57%), Postives = 411/474 (86.71%), Query Frame = 0

Query: 23  IEHSSDLTIESMMVDSDSSELDSEERAEVISLLPPYEGKTVAELGAGIGLFTAELAQRAA 82
           +EHSSDLT+E+MM+DS +S+LD EER EV+SL+PPYEGK+V ELGAGIG FT ELAQ+A 
Sbjct: 1   MEHSSDLTVEAMMLDSKASDLDKEERPEVLSLIPPYEGKSVLELGAGIGRFTGELAQKAG 60

Query: 83  QVLAVDFIEEVIKKNEIINGHHKNVKFICADVTSPELDIAEESLDLIFSNWLLMYLSDEE 142
           +V+A+DFIE  I+KNE +NGH+KN+KF+CADVTSP+L I + S+DLIFSNWLLMYLSD+E
Sbjct: 61  EVIALDFIESAIQKNESVNGHYKNIKFMCADVTSPDLKIKDGSIDLIFSNWLLMYLSDKE 120

Query: 143 VKILAERMIKWLKVGGYIFFREACFQHSENFDKKHDLSHYREPRFYSKVFKECYAKDDSG 202
           V+++AERMI W+K GGYIFFRE+CF  S +  +K + +HYREPRFY+KVF+EC  +D SG
Sbjct: 121 VELMAERMIGWVKPGGYIFFRESCFHQSGDSKRKSNPTHYREPRFYTKVFQECQTRDASG 180

Query: 203 SVFELALLGCKPTGAYLRNKKSQNQICWIWQKVHSDDDRGFQQFLDTVHYKSSGILRYER 262
           + FEL+++GCK  GAY++NKK+QNQICWIWQKV  ++D+ FQ+FLD V YKSSGILRYER
Sbjct: 181 NSFELSMVGCKCIGAYVKNKKNQNQICWIWQKVSVENDKDFQRFLDNVQYKSSGILRYER 240

Query: 263 VFGHGFVSPGGLDTTKEFVAKLELKPGQKVLDVCCGIGGADFYMAENFDVEVVGIDLSVN 322
           VFG G+VS GG +TTKEFVAK++LKPGQKVLDV CGIGG DFYMAENFDV VVGIDLSVN
Sbjct: 241 VFGEGYVSTGGFETTKEFVAKMDLKPGQKVLDVGCGIGGGDFYMAENFDVHVVGIDLSVN 300

Query: 323 MISLALERAIGLKCSVEFEVADCTKKTYPDHTFDVIYSRDAILHIQDKPALFRSFYKWLK 382
           MIS ALERAIGLKCSVEFEVADCT KTYPD++FDVIYSRD ILHIQDKPALFR+F+KWLK
Sbjct: 301 MISFALERAIGLKCSVEFEVADCTTKTYPDNSFDVIYSRDTILHIQDKPALFRTFFKWLK 360

Query: 383 PEGKLFITDYCRSAGTSSPEFAEYIKQGGYDLHDVKTYGQMIEDAGFSEVVSEDRTYQFI 442
           P GK+ ITDYCRSA T SPEFAEYIKQ GYDLHDV+ YGQM++DAGF +V++EDRT QF+
Sbjct: 361 PGGKVLITDYCRSAETPSPEFAEYIKQRGYDLHDVQAYGQMLKDAGFDDVIAEDRTDQFV 420

Query: 443 QLLQRELEAVEEQKDTFIHDFSEEGYNHIVSGWKAKRNRGLSGEQRWGLFFAKK 497
           Q+L+RELE VE++K+ FI DFSEE YN IV GW AK  R  SGEQ+WGLF A K
Sbjct: 421 QVLRRELEKVEKEKEEFISDFSEEDYNDIVGGWSAKLERTASGEQKWGLFIADK 474

BLAST of Spg022328 vs. TAIR 10
Match: AT1G73600.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 729.9 bits (1883), Expect = 1.3e-210
Identity = 345/484 (71.28%), Postives = 411/484 (84.92%), Query Frame = 0

Query: 13  EERERQKKYWIEHSSDLTIESMMVDSDSSELDSEERAEVISLLPPYEGKTVAELGAGIGL 72
           EERE QK YW EHS  L++E+MM+DS +S+LD EER E+++ LPP EG TV E GAGIG 
Sbjct: 20  EEREIQKNYWKEHSVGLSVEAMMLDSKASDLDKEERPEILAFLPPIEGTTVLEFGAGIGR 79

Query: 73  FTAELAQRAAQVLAVDFIEEVIKKNEIINGHHKNVKFICADVTSPELDIAEESLDLIFSN 132
           FT ELAQ+A QV+AVDFIE VIKKNE INGH+KNVKF+CADVTSP ++   ES+DLIFSN
Sbjct: 80  FTTELAQKAGQVIAVDFIESVIKKNENINGHYKNVKFLCADVTSPNMNFPNESMDLIFSN 139

Query: 133 WLLMYLSDEEVKILAERMIKWLKVGGYIFFREACFQHSENFDKKHDLSHYREPRFYSKVF 192
           WLLMYLSD+EV+ LA++M++W KVGGYIFFRE+CF  S +  +K++ +HYREP+FY+K+F
Sbjct: 140 WLLMYLSDQEVEDLAKKMLQWTKVGGYIFFRESCFHQSGDNKRKYNPTHYREPKFYTKLF 199

Query: 193 KECYAKDDSGSVFELALLGCKPTGAYLRNKKSQNQICWIWQKVHSDDDRGFQQFLDTVHY 252
           KEC+  D+ G+ +EL+L+ CK  GAY+RNKK+QNQICW+WQKV SD+DRGFQ+FLD V Y
Sbjct: 200 KECHMNDEDGNSYELSLVSCKCIGAYVRNKKNQNQICWLWQKVSSDNDRGFQRFLDNVQY 259

Query: 253 KSSGILRYERVFGHGFVSPGGLDTTKEFVAKLELKPGQKVLDVCCGIGGADFYMAENFDV 312
           KSSGILRYERVFG GFVS GGL+TTKEFV  L+LKPGQKVLDV CGIGG DFYMAENFDV
Sbjct: 260 KSSGILRYERVFGEGFVSTGGLETTKEFVDMLDLKPGQKVLDVGCGIGGGDFYMAENFDV 319

Query: 313 EVVGIDLSVNMISLALERAIGLKCSVEFEVADCTKKTYPDHTFDVIYSRDAILHIQDKPA 372
           +VVGIDLSVNMIS ALE AIGLKCSVEFEVADCTKK YPD+TFDVIYSRD ILHIQDKPA
Sbjct: 320 DVVGIDLSVNMISFALEHAIGLKCSVEFEVADCTKKEYPDNTFDVIYSRDTILHIQDKPA 379

Query: 373 LFRSFYKWLKPEGKLFITDYCRSAGTSSPEFAEYIKQGGYDLHDVKTYGQMIEDAGFSEV 432
           LFR FYKWLKP GK+ ITDYCRS  T SP+FA YIK+ GYDLHDV+ YGQM+ DAGF EV
Sbjct: 380 LFRRFYKWLKPGGKVLITDYCRSPKTPSPDFAIYIKKRGYDLHDVQAYGQMLRDAGFEEV 439

Query: 433 VSEDRTYQFIQLLQRELEAVEEQKDTFIHDFSEEGYNHIVSGWKAKRNRGLSGEQRWGLF 492
           ++EDRT QF+++L+REL+AVE++K+ FI DFS+E Y  I+ GWK+K  R  SGEQ+WGLF
Sbjct: 440 IAEDRTDQFMKVLKRELDAVEKEKEEFISDFSKEDYEDIIGGWKSKLLRSSSGEQKWGLF 499

Query: 493 FAKK 497
            AK+
Sbjct: 500 IAKR 503

BLAST of Spg022328 vs. TAIR 10
Match: AT1G73600.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 729.9 bits (1883), Expect = 1.3e-210
Identity = 345/484 (71.28%), Postives = 411/484 (84.92%), Query Frame = 0

Query: 13  EERERQKKYWIEHSSDLTIESMMVDSDSSELDSEERAEVISLLPPYEGKTVAELGAGIGL 72
           EERE QK YW EHS  L++E+MM+DS +S+LD EER E+++ LPP EG TV E GAGIG 
Sbjct: 6   EEREIQKNYWKEHSVGLSVEAMMLDSKASDLDKEERPEILAFLPPIEGTTVLEFGAGIGR 65

Query: 73  FTAELAQRAAQVLAVDFIEEVIKKNEIINGHHKNVKFICADVTSPELDIAEESLDLIFSN 132
           FT ELAQ+A QV+AVDFIE VIKKNE INGH+KNVKF+CADVTSP ++   ES+DLIFSN
Sbjct: 66  FTTELAQKAGQVIAVDFIESVIKKNENINGHYKNVKFLCADVTSPNMNFPNESMDLIFSN 125

Query: 133 WLLMYLSDEEVKILAERMIKWLKVGGYIFFREACFQHSENFDKKHDLSHYREPRFYSKVF 192
           WLLMYLSD+EV+ LA++M++W KVGGYIFFRE+CF  S +  +K++ +HYREP+FY+K+F
Sbjct: 126 WLLMYLSDQEVEDLAKKMLQWTKVGGYIFFRESCFHQSGDNKRKYNPTHYREPKFYTKLF 185

Query: 193 KECYAKDDSGSVFELALLGCKPTGAYLRNKKSQNQICWIWQKVHSDDDRGFQQFLDTVHY 252
           KEC+  D+ G+ +EL+L+ CK  GAY+RNKK+QNQICW+WQKV SD+DRGFQ+FLD V Y
Sbjct: 186 KECHMNDEDGNSYELSLVSCKCIGAYVRNKKNQNQICWLWQKVSSDNDRGFQRFLDNVQY 245

Query: 253 KSSGILRYERVFGHGFVSPGGLDTTKEFVAKLELKPGQKVLDVCCGIGGADFYMAENFDV 312
           KSSGILRYERVFG GFVS GGL+TTKEFV  L+LKPGQKVLDV CGIGG DFYMAENFDV
Sbjct: 246 KSSGILRYERVFGEGFVSTGGLETTKEFVDMLDLKPGQKVLDVGCGIGGGDFYMAENFDV 305

Query: 313 EVVGIDLSVNMISLALERAIGLKCSVEFEVADCTKKTYPDHTFDVIYSRDAILHIQDKPA 372
           +VVGIDLSVNMIS ALE AIGLKCSVEFEVADCTKK YPD+TFDVIYSRD ILHIQDKPA
Sbjct: 306 DVVGIDLSVNMISFALEHAIGLKCSVEFEVADCTKKEYPDNTFDVIYSRDTILHIQDKPA 365

Query: 373 LFRSFYKWLKPEGKLFITDYCRSAGTSSPEFAEYIKQGGYDLHDVKTYGQMIEDAGFSEV 432
           LFR FYKWLKP GK+ ITDYCRS  T SP+FA YIK+ GYDLHDV+ YGQM+ DAGF EV
Sbjct: 366 LFRRFYKWLKPGGKVLITDYCRSPKTPSPDFAIYIKKRGYDLHDVQAYGQMLRDAGFEEV 425

Query: 433 VSEDRTYQFIQLLQRELEAVEEQKDTFIHDFSEEGYNHIVSGWKAKRNRGLSGEQRWGLF 492
           ++EDRT QF+++L+REL+AVE++K+ FI DFS+E Y  I+ GWK+K  R  SGEQ+WGLF
Sbjct: 426 IAEDRTDQFMKVLKRELDAVEKEKEEFISDFSKEDYEDIIGGWKSKLLRSSSGEQKWGLF 485

Query: 493 FAKK 497
            AK+
Sbjct: 486 IAKR 489