Spg022312 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg022312
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	isoleucine N-monooxygenase 1-like
Location	scaffold2: 13465643 .. 13468324 (+)
RNA-Seq Expression	Spg022312
Synteny	Spg022312

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTATTCGATAGAAGATACTACAACGATGGAGGGCCAACTTTCGAAGAAGAAGAGGAGCACATTCAAGCATTGCTAACGCTGCTTGAGCATGTTCATGCATTTTCTGTATCTGATTACGTGCCTTGTTTGAAGTTGTTTGATTTGAACGGACACAAGAAGACTATAAAACAAGCTTTCGAAGTAATCAAGAAGCATGACGATTCCATTATAGATGAGAGAGTGCAGCAATGGAAGGATGAGAAGAGAAAAGAGGTGGATGATATTCTTGATATTTTCATCTCGCTTACAGATGAAAATGGAAAGTCGCTATTATCAATCCAAGAGATTAAAGCAAAAATCATGGTGAGAATTTCTTGGCATGTATTCAACCACATCATTATGAACAATACTTGGTTGCATACCGGTATATAGGGATTTCCATGCATGGCGCTATTAAAATGTTGATAATTAGGAGAGATAAAAAAGTTTTATCATTTTCTACCATTCTCTTTCTTAGTTGTCAAGATTTTGGTGGGGTATGTAGGGAGAATATTCCTACATATCAGTATGTAGCCAAGTATTTTTCTATCATTATTAATTAGAGGAAAAAACTTGGATGCATACTAGGGATCTCTTTACATACCACACCATTAAAATCTTTACAACTAGGAGAGATAAAAAAAAAATCTCATTTTTTACCCTTATCTTTCTAAGTTGTCAAGATTTTAATGGGGCCCTACATACCGATATGCATTCTTAGTTAAATTGCATGTGCTATCGTATATAACCATTCTCTATAAATATAACATTATTTATTAGGGGTGAGCATCACAAACCGAAAAATCGAGCCGACCGAACGAAACCGATCAAACCGACGTTGGTCAGTCGATTTTCTAGCTGTGTTGGTCGGAGTCAGTTTTTTCATAGGAAAAACCGACCATTCGGTCGGTCGGCGAGCCAAAAAAGGATCAAACCGACCGAAACACACCAATTTTGTATTTAAAAAAATAAATATATATATATTTAAAAAAAAAACTGTAGACTTGACATCTTTATTTTGAAAAGAAGAAAAAACCAGCTTACATCAATCTTGTCACCTTTTATATAGGTAATGATTACCTATTTTTTAGGTAATCTTTACTTATTTTAAAAAAATTATTTTTTAATTTTATCTTTCTTCATCAGCATCACATCTCACGTTTTCTTCCTCTTCCCTTTGTCAGACTTTCCTCTTTCTTTCTCTTCTTCTTACTCTCTCATTTCCTCTCGTTTTCTTTTCCTCTTTCTTTCTCTTCTTCCCACTCTCATCTCTTTTCCTCTCTTTTTTTCTTTTTCTCTTTGATCTTCTTTGTCGGAACCGCCTCTTTGCCGGAGTTTATCGCCGCCACGAAGGTTGTTTCTTTCTCTTCGCTGAAACCTTACGTCTCTCTTCACCTTATTTTCATATCTTTGTTGGTTCCTAACACACTCTTTTATCTTTCTCTCCCAACGCCTTCTATCATTCTCTTTTCTTCTCATAAGCTCCGGTTAACTTTCAAAACCGACCGAAATTAACCGATGTGTATGTATGGTTGGTCGGTCGATTTTATGGACATCTCCGAGACTGACTACGCCGACCGAACAAAATCGACTTGTCGGTGATCGGCGTCGATTTGAGCCCAAAACCGACGCCGACCGATCGATGATCACCCCTATTATTTATTCTTTGCCAATAAGAATATAACTCAACTGGGCATACTTATGACCAATATTCATAGGTTCAAATCCCCTAATCCTATATTGTTGAATTAAAAAAAACATTTTTTATTCTTGGTCTTTTGCATATTTATTCTTTATAAGCTAAAATATTTTATTTTGGTAAATGCTTATAATTAAAGAAAAAAAGAAAAAGAAAGAAAGATTGGAGGGAATTTGGTAGAAAGAATTAAAAATAAATTTAAAAAAAAATGGTTAACCCCACCTGTTTAATACCTCACATGTCACAAAATAAGGAGGGAGCCTCATTGTGGCTGGATCACCTAATAATTTCTCCTCGTGGACGATATATATGTTTAATCATTTTCCTAATGACAGGAGTTACAAATGACAACGATTGGTAATCCTTCAAACGGAGTGGAATGGGCAATGGCGGAGTTATTCAATCAACCAAAAATTCTCCAACAAGCAATAGAGGAATTGGATAGGATAGTTGGAAAAGACAGACTTGTTCAAGAATCCGACATCCCACAACTTAAGTATCTCATAGCTTGTATCAGAGAATCTTTTCGACTTCATACATTTTCACCTTTCAATGTGCCTCATGTCTCAAATTCTGATGCCATTGTAGCTGGTTACTTCATTCCAAAAGGTAGTCAAGTCTTGCTGAGTCGTTTGGGGCTTGGTCGAAATCCGAGGATTTGGGACGATCCATTGAGTTACAATCCACAACGTCATCTCAACGATGAGACAGTTGAATTAGGATTATCCGAACCGAGTCTACGCTTCATTAGCTTCTCCAGAGGGAGAAGAGGTTGCATAGGTAGCTCACTAGGCACAACCATTACCATGATGCTCTTTGCTAGGCTGCTACAAGGGTTTTCATGGAGCTTAATGCCTGGAACCACAAAGGTAAACTTCTCTCACTTTGACGAGCTCGCCCTTCCAAACCCACTCTACTTGTATGTAAAGCCACGTCTATCTCATAATATGTATCCTAGTTAG

mRNA sequence

ATGTTATTCGATAGAAGATACTACAACGATGGAGGGCCAACTTTCGAAGAAGAAGAGGAGCACATTCAAGCATTGCTAACGCTGCTTGAGCATGTTCATGCATTTTCTGTATCTGATTACGTGCCTTGTTTGAAGTTGTTTGATTTGAACGGACACAAGAAGACTATAAAACAAGCTTTCGAAGTAATCAAGAAGCATGACGATTCCATTATAGATGAGAGAGTGCAGCAATGGAAGGATGAGAAGAGAAAAGAGGTGGATGATATTCTTGATATTTTCATCTCGCTTACAGATGAAAATGGAAAGTCGCTATTATCAATCCAAGAGATTAAAGCAAAAATCATGGAGTTACAAATGACAACGATTGGTAATCCTTCAAACGGAGTGGAATGGGCAATGGCGGAGTTATTCAATCAACCAAAAATTCTCCAACAAGCAATAGAGGAATTGGATAGGATAGTTGGAAAAGACAGACTTGTTCAAGAATCCGACATCCCACAACTTAAGTATCTCATAGCTTGTATCAGAGAATCTTTTCGACTTCATACATTTTCACCTTTCAATGTGCCTCATGTCTCAAATTCTGATGCCATTGTAGCTGGTTACTTCATTCCAAAAGGTAGTCAAGTCTTGCTGAGTCGTTTGGGGCTTGGTCGAAATCCGAGGATTTGGGACGATCCATTGAGTTACAATCCACAACGTCATCTCAACGATGAGACAGTTGAATTAGGATTATCCGAACCGAGTCTACGCTTCATTAGCTTCTCCAGAGGGAGAAGAGGTTGCATAGGTAGCTCACTAGGCACAACCATTACCATGATGCTCTTTGCTAGGCTGCTACAAGGGTTTTCATGGAGCTTAATGCCTGGAACCACAAAGGTAAACTTCTCTCACTTTGACGAGCTCGCCCTTCCAAACCCACTCTACTTGTATGTAAAGCCACGTCTATCTCATAATATGTATCCTAGTTAG

Coding sequence (CDS)

ATGTTATTCGATAGAAGATACTACAACGATGGAGGGCCAACTTTCGAAGAAGAAGAGGAGCACATTCAAGCATTGCTAACGCTGCTTGAGCATGTTCATGCATTTTCTGTATCTGATTACGTGCCTTGTTTGAAGTTGTTTGATTTGAACGGACACAAGAAGACTATAAAACAAGCTTTCGAAGTAATCAAGAAGCATGACGATTCCATTATAGATGAGAGAGTGCAGCAATGGAAGGATGAGAAGAGAAAAGAGGTGGATGATATTCTTGATATTTTCATCTCGCTTACAGATGAAAATGGAAAGTCGCTATTATCAATCCAAGAGATTAAAGCAAAAATCATGGAGTTACAAATGACAACGATTGGTAATCCTTCAAACGGAGTGGAATGGGCAATGGCGGAGTTATTCAATCAACCAAAAATTCTCCAACAAGCAATAGAGGAATTGGATAGGATAGTTGGAAAAGACAGACTTGTTCAAGAATCCGACATCCCACAACTTAAGTATCTCATAGCTTGTATCAGAGAATCTTTTCGACTTCATACATTTTCACCTTTCAATGTGCCTCATGTCTCAAATTCTGATGCCATTGTAGCTGGTTACTTCATTCCAAAAGGTAGTCAAGTCTTGCTGAGTCGTTTGGGGCTTGGTCGAAATCCGAGGATTTGGGACGATCCATTGAGTTACAATCCACAACGTCATCTCAACGATGAGACAGTTGAATTAGGATTATCCGAACCGAGTCTACGCTTCATTAGCTTCTCCAGAGGGAGAAGAGGTTGCATAGGTAGCTCACTAGGCACAACCATTACCATGATGCTCTTTGCTAGGCTGCTACAAGGGTTTTCATGGAGCTTAATGCCTGGAACCACAAAGGTAAACTTCTCTCACTTTGACGAGCTCGCCCTTCCAAACCCACTCTACTTGTATGTAAAGCCACGTCTATCTCATAATATGTATCCTAGTTAG

Protein sequence

MLFDRRYYNDGGPTFEEEEEHIQALLTLLEHVHAFSVSDYVPCLKLFDLNGHKKTIKQAFEVIKKHDDSIIDERVQQWKDEKRKEVDDILDIFISLTDENGKSLLSIQEIKAKIMELQMTTIGNPSNGVEWAMAELFNQPKILQQAIEELDRIVGKDRLVQESDIPQLKYLIACIRESFRLHTFSPFNVPHVSNSDAIVAGYFIPKGSQVLLSRLGLGRNPRIWDDPLSYNPQRHLNDETVELGLSEPSLRFISFSRGRRGCIGSSLGTTITMMLFARLLQGFSWSLMPGTTKVNFSHFDELALPNPLYLYVKPRLSHNMYPS

Homology

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BLAST of Spg022312 vs. NCBI nr
Match: XP_023516709.1 (isoleucine N-monooxygenase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 490.7 bits (1262), Expect = 9.5e-135
Identity = 239/327 (73.09%), Postives = 281/327 (85.93%), Query Frame = 0


Query: 1   MLFDRRYY----NDGGPTFEEEEEHIQALLTLLEHVHAFSVSDYVPCLKLFDLNGHKKTI 60
           MLF++RYY     DGGPTF EEEEH QALLT+L HV+AFS+SD++P LK FDL+GH+K +
Sbjct: 210 MLFNQRYYGKGREDGGPTF-EEEEHNQALLTILRHVNAFSISDFLPWLKPFDLDGHQKVL 269

Query: 61  KQAFEVIKKHDDSIIDERVQQWKDEKRKEV-DDILDIFISLTDENGKSLLSIQEIKAKIM 120
           K A  V++K+D+ IIDERVQ+WKDEK +EV DDILDI ISL DEN K LLS+ EIKA+I 
Sbjct: 270 KNALNVVQKYDEPIIDERVQRWKDEKLEEVKDDILDILISLKDENAKPLLSVAEIKAQIT 329

Query: 121 ELQMTTIGNPSNGVEWAMAELFNQPKILQQAIEELDRIVGKDRLVQESDIPQLKYLIACI 180
           ELQ+ TI NPSN VEWAMAEL NQPKIL++AIEELD++VG++RLV+E DIP LKYL ACI
Sbjct: 330 ELQIATIDNPSNAVEWAMAELLNQPKILEKAIEELDKVVGRERLVEEYDIPNLKYLTACI 389

Query: 181 RESFRLHTFSPFNVPHVSNSDAIVAGYFIPKGSQVLLSRLGLGRNPRIWDDPLSYNPQRH 240
           RESFRLH FSPFN+PHVSNSD IVAGYFIPKGS+VLLSR+GLGRNP+IW+DP+ +NP+RH
Sbjct: 390 RESFRLHPFSPFNIPHVSNSDIIVAGYFIPKGSEVLLSRIGLGRNPKIWEDPMKFNPERH 449

Query: 241 LNDETVELGLSEPSLRFISFSRGRRGCIGSSLGTTITMMLFARLLQGFSWSLMPGTTKVN 300
           +ND T ELGL EP LRF+SFSRGRRGC GSSLGT ITMMLFARLLQGFSWSL+ G TK++
Sbjct: 450 MNDRTTELGLLEPDLRFMSFSRGRRGCPGSSLGTNITMMLFARLLQGFSWSLLRGITKID 509

Query: 301 FSHFDELALPNPLYLYVKPRLSHNMYP 323
           FS  DEL+LP PL+L+ KPRLSH MYP
Sbjct: 510 FSEADELSLPKPLHLHAKPRLSHIMYP 535

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BLAST of Spg022312 vs. NCBI nr
Match: XP_038878794.1 (tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida])

HSP 1 Score: 488.8 bits (1257), Expect = 3.6e-134
Identity = 234/329 (71.12%), Postives = 288/329 (87.54%), Query Frame = 0


Query: 1   MLFDRRYY----NDGGPTFEEEEEHIQALLTLLEHVHAFSVSDYVPCLKLFDLNGHKKTI 60
           MLF+RRYY     DGGPTF EEE+H QALLT+L HV+AFS+SD+VPCLK FDL+GH+K +
Sbjct: 217 MLFNRRYYGKGREDGGPTF-EEEDHNQALLTILRHVNAFSISDFVPCLKPFDLDGHEKIM 276

Query: 61  KQAFEVIKKHDDSIIDERVQQWKDEKRKEVDDILDIFISLTDENGKSLLSIQEIKAKIME 120
           K+A  +++K+D+ IIDERVQ WK +K + ++DILDI ISL D+NG SLLSI+EIKA+I+E
Sbjct: 277 KEALNLVRKYDEPIIDERVQHWKVKKIRVIEDILDILISLKDDNGNSLLSIEEIKAQIVE 336

Query: 121 LQMTTIGNPSNGVEWAMAELFNQPKILQQAIEELDRIVGKDRLVQESDIPQLKYLIACIR 180
           LQ+ TI NPSN VEWAMAEL NQP++L++A+EELD +VG++RLV+ESDIP+LKYL ACIR
Sbjct: 337 LQIATIDNPSNAVEWAMAELINQPEVLKKAVEELDEVVGRERLVEESDIPKLKYLTACIR 396

Query: 181 ESFRLHTFSPFNVPHVSNSDAIVAGYFIPKGSQVLLSRLGLGRNPRIWDDPLSYNPQRHL 240
           ESFRLH FSPFN+PHVSNSD +V GY IPKGS+VLLSR+GLGRNPRIW+DP+ +NP+RHL
Sbjct: 397 ESFRLHPFSPFNIPHVSNSDIVVNGYLIPKGSEVLLSRIGLGRNPRIWEDPMRFNPERHL 456

Query: 241 NDETV-ELGLSEPSLRFISFSRGRRGCIGSSLGTTITMMLFARLLQGFSWSLMPGTTKVN 300
            D+TV ELG+SEPSLRFI+F+RGRRGC+GSSLGT ITMMLFARLLQGF WSL+PG TK++
Sbjct: 457 KDDTVTELGISEPSLRFITFTRGRRGCVGSSLGTNITMMLFARLLQGFFWSLLPGITKMD 516

Query: 301 FSHFD-ELALPNPLYLYVKPRLSHNMYPS 324
           FS  D EL+LP PL LYV+PRLSH+MYP+
Sbjct: 517 FSEIDHELSLPTPLQLYVQPRLSHSMYPN 544

[Close]

BLAST of Spg022312 vs. NCBI nr
Match: XP_038880438.1 (tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida])

HSP 1 Score: 488.0 bits (1255), Expect = 6.1e-134
Identity = 230/327 (70.34%), Postives = 283/327 (86.54%), Query Frame = 0


Query: 1   MLFDRRYY----NDGGPTFEEEEEHIQALLTLLEHVHAFSVSDYVPCLKLFDLNGHKKTI 60
           MLF+RRYY     DGGPTF EEEEH+QALLT+++H+HAFS+SD++PCLK FDL+GH+K +
Sbjct: 218 MLFNRRYYGKGREDGGPTF-EEEEHVQALLTIVKHIHAFSISDFMPCLKSFDLDGHQKIM 277

Query: 61  KQAFEVIKKHDDSIIDERVQQWKDEKRKEVDDILDIFISLTDENGKSLLSIQEIKAKIME 120
           K A  +++K+D+ IIDERVQ+WKD+K KEV+DILDI ISL DEN  SLLSI+EIKA+I +
Sbjct: 278 KNALNILQKYDEPIIDERVQEWKDKKIKEVEDILDILISLKDENTNSLLSIEEIKAQITD 337

Query: 121 LQMTTIGNPSNGVEWAMAELFNQPKILQQAIEELDRIVGKDRLVQESDIPQLKYLIACIR 180
           LQ+ TI NPSN VEWAMAEL NQPKIL++ +EELD++VG++RLVQESDIP+LKYL AC R
Sbjct: 338 LQIATIDNPSNAVEWAMAELLNQPKILEKVVEELDKVVGRERLVQESDIPKLKYLTACAR 397

Query: 181 ESFRLHTFSPFNVPHVSNSDAIVAGYFIPKGSQVLLSRLGLGRNPRIWDDPLSYNPQRHL 240
           ESFRLH FS FNVPH+S SD +VAGYFIPKGS+VLLSR GLGRNPR+W+DP+ ++P+RHL
Sbjct: 398 ESFRLHPFSSFNVPHISTSDIVVAGYFIPKGSEVLLSRSGLGRNPRVWEDPMRFDPERHL 457

Query: 241 NDETVELGLSEPSLRFISFSRGRRGCIGSSLGTTITMMLFARLLQGFSWSLMPGTTKVNF 300
            D TVELG+SEP+LRFI+F+RGRRGC GSSLGT ITMMLFARLLQGF+WSLM G TK++ 
Sbjct: 458 KDGTVELGISEPNLRFITFTRGRRGCAGSSLGTNITMMLFARLLQGFTWSLMAGITKIDL 517

Query: 301 SHFDELALPNPLYLYVKPRLSHNMYPS 324
           S   +L+L  PL+L+ KPRLSH+MYPS
Sbjct: 518 SDTGDLSLSKPLHLHAKPRLSHSMYPS 543

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BLAST of Spg022312 vs. NCBI nr
Match: XP_022960607.1 (isoleucine N-monooxygenase 1-like [Cucurbita moschata])

HSP 1 Score: 488.0 bits (1255), Expect = 6.1e-134
Identity = 241/327 (73.70%), Postives = 279/327 (85.32%), Query Frame = 0


Query: 1   MLFDRRYY----NDGGPTFEEEEEHIQALLTLLEHVHAFSVSDYVPCLKLFDLNGHKKTI 60
           MLF++RYY     DGGPTF EEEEH QA LT++ H+HAFS+SD++  LK FDL+GH+K +
Sbjct: 196 MLFNQRYYGKWREDGGPTF-EEEEHDQASLTIIRHLHAFSISDFMSWLKPFDLDGHEKVM 255

Query: 61  KQAFEVIKKHDDSIIDERVQQWKDEKRKEV-DDILDIFISLTDENGKSLLSIQEIKAKIM 120
           K A  V++K D+ IIDERVQ+WKDEK +EV DDILDI ISL DEN K LLS+ EIKA+IM
Sbjct: 256 KSALNVVQKFDEPIIDERVQRWKDEKLEEVKDDILDILISLKDENAKPLLSVVEIKAQIM 315

Query: 121 ELQMTTIGNPSNGVEWAMAELFNQPKILQQAIEELDRIVGKDRLVQESDIPQLKYLIACI 180
           ELQ+ TI NPSN VEWAMAEL NQPKIL++AIEELD++VG++RLVQE DIP LKYL ACI
Sbjct: 316 ELQIATIDNPSNAVEWAMAELLNQPKILEKAIEELDKVVGRERLVQEYDIPNLKYLTACI 375

Query: 181 RESFRLHTFSPFNVPHVSNSDAIVAGYFIPKGSQVLLSRLGLGRNPRIWDDPLSYNPQRH 240
           RESFRLH FSPFNVPHVSN+D IVAGYFIPKGS+VLLSR GLGRNPRIW+DP+ +N +RH
Sbjct: 376 RESFRLHPFSPFNVPHVSNTDIIVAGYFIPKGSEVLLSRTGLGRNPRIWEDPMRFNRERH 435

Query: 241 LNDETVELGLSEPSLRFISFSRGRRGCIGSSLGTTITMMLFARLLQGFSWSLMPGTTKVN 300
           LND TVELGL EP+LRF+SFSRGRRGC GSSLGT ITMMLFARLLQGFSWSL+PG TK++
Sbjct: 436 LNDRTVELGLLEPNLRFMSFSRGRRGCPGSSLGTNITMMLFARLLQGFSWSLLPGITKID 495

Query: 301 FSHFDELALPNPLYLYVKPRLSHNMYP 323
           FS  DEL+LP PL+L  KPRLSH MYP
Sbjct: 496 FSQADELSLPKPLHLLAKPRLSHVMYP 521

[Close]

BLAST of Spg022312 vs. NCBI nr
Match: KAG6590174.1 (Isoleucine N-monooxygenase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 487.3 bits (1253), Expect = 1.0e-133
Identity = 239/327 (73.09%), Postives = 281/327 (85.93%), Query Frame = 0


Query: 1   MLFDRRYY----NDGGPTFEEEEEHIQALLTLLEHVHAFSVSDYVPCLKLFDLNGHKKTI 60
           MLF++RYY     DGGPTF EEEEH QALLT++ H+HAFS+SD++  LK FDL+ H+K +
Sbjct: 196 MLFNQRYYGKWREDGGPTF-EEEEHDQALLTIIRHLHAFSISDFMSWLKPFDLDEHEKVM 255

Query: 61  KQAFEVIKKHDDSIIDERVQQWKDEKRKEV-DDILDIFISLTDENGKSLLSIQEIKAKIM 120
           K A  VI+K+D+ IIDERVQ+WKDEK +EV DDILDI ISL DEN K LLS+ EIKA+IM
Sbjct: 256 KNALNVIQKYDEPIIDERVQRWKDEKLEEVKDDILDILISLKDENAKPLLSVVEIKAQIM 315

Query: 121 ELQMTTIGNPSNGVEWAMAELFNQPKILQQAIEELDRIVGKDRLVQESDIPQLKYLIACI 180
           ELQ+ TI NPSN VEWAMAEL NQPKIL++AIEELD++VG++RLV+E DIP LKYL ACI
Sbjct: 316 ELQIATIDNPSNAVEWAMAELLNQPKILEKAIEELDKVVGRERLVEEYDIPNLKYLTACI 375

Query: 181 RESFRLHTFSPFNVPHVSNSDAIVAGYFIPKGSQVLLSRLGLGRNPRIWDDPLSYNPQRH 240
           RESFRLH FSPFNVPHVSN+D IVAG+FIPKGS+VLLSR GLGRNPRIW+DP+ +NP+RH
Sbjct: 376 RESFRLHPFSPFNVPHVSNTDIIVAGFFIPKGSEVLLSRTGLGRNPRIWEDPMRFNPERH 435

Query: 241 LNDETVELGLSEPSLRFISFSRGRRGCIGSSLGTTITMMLFARLLQGFSWSLMPGTTKVN 300
           LND TVELGL EP+LRF+SFSRGRRGC GSSLGT ITMMLFARLLQGFSWS +PG TK++
Sbjct: 436 LNDRTVELGLLEPNLRFMSFSRGRRGCPGSSLGTNITMMLFARLLQGFSWSFVPGITKID 495

Query: 301 FSHFDELALPNPLYLYVKPRLSHNMYP 323
           FS  DEL+LP PL+L+ +PRLSH MYP
Sbjct: 496 FSEADELSLPKPLHLHAQPRLSHIMYP 521

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BLAST of Spg022312 vs. ExPASy Swiss-Prot
Match: A0A068Q605 (Tryptophan N-monooxygenase CYP79A68 OS=Prunus mume OX=102107 GN=CYP79A68 PE=1 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 5.8e-103
Identity = 185/331 (55.89%), Postives = 249/331 (75.23%), Query Frame = 0


Query: 1   MLFDRRYY----NDGGPTFEEEEEHIQALLTLLEHVHAFSVSDYVPCLKLFDLNGHKKTI 60
           M+F+R Y+     DGGP   EEEEH+ ALLTLL + +AF VSDY+P L++FD++GH+K +
Sbjct: 206 MIFNRTYFGKGREDGGPGV-EEEEHVSALLTLLTYAYAFCVSDYLPWLRVFDIDGHEKKV 265

Query: 61  KQAFEVIKKHDDSIIDERVQQWKDEKRKEVDDILDIFISLTDENGKSLLSIQEIKAKIME 120
           ++A  ++KKH + I++ER+Q+W+D KR E DD+LD+FISL D NG+ LLS +EIKA+  E
Sbjct: 266 RKAMNIVKKHQEPIVNERLQEWRDGKRNEPDDLLDVFISLKDANGQPLLSDEEIKAQTTE 325

Query: 121 LQMTTIGNPSNGVEWAMAELFNQPKILQQAIEELDRIVGKDRLVQESDIPQLKYLIACIR 180
           LQ++T+ +P N  EWA+ E+ NQP++L++A EELDR+VGK  LVQES +P L Y+ AC +
Sbjct: 326 LQLSTVDSPFNIAEWALTEMLNQPEMLKKAEEELDRVVGKKTLVQESHVPHLPYIRACAK 385

Query: 181 ESFRLHTFSPFNVPHVSNSDAIVAGYFIPKGSQVLLSRLGLGRNPRIWDDPLSYNPQRHL 240
           E  RLH   PFN+PHVS +DA VAGYFIPKGS V+LSRL LGRNPR+W++PL +NP+RHL
Sbjct: 386 EVMRLHPVGPFNLPHVSIADAEVAGYFIPKGSNVILSRLELGRNPRVWEEPLRFNPERHL 445

Query: 241 N---DETVELGLSEPSLRFISFSRGRRGCIGSSLGTTITMMLFARLLQGFSWSLMPGTTK 300
           N   D+ V+  L E  LRF+SFS GRRGC+G  LG+TI +ML ARLLQGFSWSL P   K
Sbjct: 446 NIAVDQQVD--LEENDLRFVSFSTGRRGCMGVGLGSTIVVMLLARLLQGFSWSLPPDVDK 505

Query: 301 VNFSHFDELAL--PNPLYLYVKPRLSHNMYP 323
           ++F+  D++ L   +PL    KPRL  ++YP
Sbjct: 506 IDFTE-DQIYLKKASPLLAQAKPRLPASVYP 532

[Close]

BLAST of Spg022312 vs. ExPASy Swiss-Prot
Match: Q9FLC8 (Phenylalanine N-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP79A2 PE=1 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 9.0e-96
Identity = 162/328 (49.39%), Postives = 233/328 (71.04%), Query Frame = 0


Query: 1   MLFDRRYY----NDGGPTFEEEEEHIQALLTLLEHVHAFSVSDYVPCLKLFDLNGHKKTI 60
           M+F  R++     DG     EE EH+++L T+L H++AF++SDYVP L+  DL GH+K +
Sbjct: 203 MMFGIRHFGKGSEDGSGPGLEEIEHVESLFTVLTHLYAFALSDYVPWLRFLDLEGHEKVV 262

Query: 61  KQAFEVIKKHDDSIIDERVQQWKDEKRKEVDDILDIFISLTDENGKSLLSIQEIKAKIME 120
             A   + K++D  +DER+ QW++ K KE  D LD+FI   D +GK  LS +EIKA++ E
Sbjct: 263 SNAMRNVSKYNDPFVDERLMQWRNGKMKEPQDFLDMFIIAKDTDGKPTLSDEEIKAQVTE 322

Query: 121 LQMTTIGNPSNGVEWAMAELFNQPKILQQAIEELDRIVGKDRLVQESDIPQLKYLIACIR 180
           L + T+ NPSN  EW MAE+ N+P I+Q+A+EE+DR+VGKDRLV ESD+P L Y+ AC++
Sbjct: 323 LMLATVDNPSNAAEWGMAEMINEPSIMQKAVEEIDRVVGKDRLVIESDLPNLNYVKACVK 382

Query: 181 ESFRLHTFSPFNVPHVSNSDAIVAGYFIPKGSQVLLSRLGLGRNPRIWDDPLSYNPQRHL 240
           E+FRLH  +PFN+PH+S +D +V GYFIPKGS VL+SR+G+GRNP +WD P  ++P+RHL
Sbjct: 383 EAFRLHPVAPFNLPHMSTTDTVVDGYFIPKGSHVLISRMGIGRNPSVWDKPHKFDPERHL 442

Query: 241 NDETVELGLSEPSLRFISFSRGRRGCIGSSLGTTITMMLFARLLQGFSWSLMPGTTKVNF 300
           +  T  + L+E  L  ISFS GRRGC+G  +G+ +T ML ARL+QGF+W  +PG  K++ 
Sbjct: 443 STNTC-VDLNESDLNIISFSAGRRGCMGVDIGSAMTYMLLARLIQGFTWLPVPGKNKIDI 502

Query: 301 SHF-DELALPNPLYLYVKPRLSHNMYPS 324
           S   ++L +  PLY    PRL+ ++YP+
Sbjct: 503 SESKNDLFMAKPLYAVATPRLAPHVYPT 529

[Close]

BLAST of Spg022312 vs. ExPASy Swiss-Prot
Match: Q6J541 (Isoleucine N-monooxygenase 1 OS=Lotus japonicus OX=34305 GN=CYP79D3 PE=1 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 2.0e-95
Identity = 168/326 (51.53%), Postives = 237/326 (72.70%), Query Frame = 0


Query: 1   MLFDRRYY----NDGGPTFEEEEEHIQALLTLLEHVHAFSVSDYVPCLKLFDLNGHKKTI 60
           ++F+ RY+     DGGP F EE EHI A  T+L++V+AFS+SD+VP L+  DL+GH+  I
Sbjct: 207 LIFNSRYFGKVMEDGGPGF-EEVEHINATFTILKYVYAFSISDFVPFLRRLDLDGHRSKI 266

Query: 61  KQAFEVIKKHDDSIIDERVQQWKDEKRKEVDDILDIFISLTDENGKSLLSIQEIKAKIME 120
            +A  +++K+ D IID+R++QW D  +   +D+LD+ I L D N K LL+++E+KA+I+E
Sbjct: 267 MKAMRIMRKYHDPIIDDRIKQWNDGLKTVEEDLLDVLIKLKDANNKPLLTLKELKAQIIE 326

Query: 121 LQMTTIGNPSNGVEWAMAELFNQPKILQQAIEELDRIVGKDRLVQESDIPQLKYLIACIR 180
           L +  + NPSN  EWA+AE+ NQP++L++A EELD +VGK+RLVQESDIP+L+++ AC R
Sbjct: 327 LAIEMVDNPSNAFEWALAEMINQPELLKRATEELDNVVGKERLVQESDIPKLQFVKACAR 386

Query: 181 ESFRLHTFSPFNVPHVSNSDAIVAGYFIPKGSQVLLSRLGLGRNPRIWDDPLSYNPQRHL 240
           E+ RLH    FNVPH+  +D +V  Y  PKG+QVLLSR+ LGRNP+ W DPL +NP+RHL
Sbjct: 387 EALRLHPMEYFNVPHLCMNDTMVGDYLFPKGTQVLLSRVALGRNPKFWTDPLKFNPERHL 446

Query: 241 NDETVELGLSEPSLRFISFSRGRRGCIGSSLGTTITMMLFARLLQGFSWSLMPGTTKVNF 300
             E +++ L+EP LRFISF+ GRR C G +LGTT+T+MLFAR+L GFSWS  P  + ++ 
Sbjct: 447 K-EGIDVVLTEPDLRFISFTTGRRSCPGVALGTTMTVMLFARMLHGFSWSPPPDVSSIDL 506

Query: 301 -SHFDELALPNPLYLYVKPRLSHNMY 322
               D+L L  PL L  KPRL+  +Y
Sbjct: 507 VPSKDDLFLAKPLLLVAKPRLAAELY 530

[Close]

BLAST of Spg022312 vs. ExPASy Swiss-Prot
Match: Q43135 (Tyrosine N-monooxygenase OS=Sorghum bicolor OX=4558 GN=CYP79A1 PE=1 SV=3)

HSP 1 Score: 347.4 bits (890), Expect = 1.7e-94
Identity = 166/328 (50.61%), Postives = 236/328 (71.95%), Query Frame = 0


Query: 1   MLFDRRYYN----DGGPTFEEEEEHIQALLTLLEHVHAFSVSDYVPCLKLFDLNGHKKTI 60
           ++F+RRY+     DGGP    E  H+ A+ T L  ++AF VSDY+P L+  DL+GH+K +
Sbjct: 229 LMFNRRYFGEPQADGGPG-PMEVLHMDAVFTSLGLLYAFCVSDYLPWLRGLDLDGHEKIV 288

Query: 61  KQAFEVIKKHDDSIIDERVQQWKDEKRKEVDDILDIFISLTDENGKSLLSIQEIKAKIME 120
           K+A   + +  D++ID+R +QWK  +R+E++D LD+ I+L D  G  LL+I+E+KA+  +
Sbjct: 289 KEANVAVNRLHDTVIDDRWRQWKSGERQEMEDFLDVLITLKDAQGNPLLTIEEVKAQSQD 348

Query: 121 LQMTTIGNPSNGVEWAMAELFNQPKILQQAIEELDRIVGKDRLVQESDIPQLKYLIACIR 180
           +    + NPSN VEWA+AE+ N P+++ +A+EELDR+VG++RLVQESDIP+L Y+ ACIR
Sbjct: 349 ITFAAVDNPSNAVEWALAEMVNNPEVMAKAMEELDRVVGRERLVQESDIPKLNYVKACIR 408

Query: 181 ESFRLHTFSPFNVPHVSNSDAIVAGYFIPKGSQVLLSRLGLGRNPRIWDDPLSYNPQRHL 240
           E+FRLH  +PFNVPHV+ +D  +AGY +PKGS V+LSR GLGRNPR+WD+PL + P RHL
Sbjct: 409 EAFRLHPVAPFNVPHVALADTTIAGYRVPKGSHVILSRTGLGRNPRVWDEPLRFYPDRHL 468

Query: 241 NDETVELGLSEPSLRFISFSRGRRGCIGSSLGTTITMMLFARLLQGFSWSLMPGTTKVNF 300
                ++ L+E  LRFISFS GRRGCI +SLGT +++MLF RLLQGF+WS   G   V+ 
Sbjct: 469 ATAASDVALTENDLRFISFSTGRRGCIAASLGTAMSVMLFGRLLQGFTWSKPAGVEAVDL 528

Query: 301 SHF-DELALPNPLYLYVKPRLSHNMYPS 324
           S    +  +  PL L+ +PRL  ++YPS
Sbjct: 529 SESKSDTFMATPLVLHAEPRLPAHLYPS 555

[Close]

BLAST of Spg022312 vs. ExPASy Swiss-Prot
Match: Q9M7B7 (Valine N-monooxygenase 2 OS=Manihot esculenta OX=3983 GN=CYP79D2 PE=1 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 2.2e-94
Identity = 167/328 (50.91%), Postives = 237/328 (72.26%), Query Frame = 0


Query: 1   MLFDRRYYN----DGGPTFEEEEEHIQALLTLLEHVHAFSVSDYVPCLKLFDLNGHKKTI 60
           M+F +RY+     DGGP   EE  H+ A+ T L++++ F +SDY+P L+  DL+G +K +
Sbjct: 215 MMFSKRYFGKGMPDGGPG-PEEIMHVDAIFTALKYLYGFCISDYLPFLEGLDLDGQEKIV 274

Query: 61  KQAFEVIKKHDDSIIDERVQQWKDEKRKEVDDILDIFISLTDENGKSLLSIQEIKAKIME 120
             A + I+   + +I+ER+QQW+  +RKE++D+LD+FI+L D +GK LL+  EIK +I E
Sbjct: 275 LNANKTIRDLQNPLIEERIQQWRSGERKEMEDLLDVFITLQDSDGKPLLNPDEIKNQIAE 334

Query: 121 LQMTTIGNPSNGVEWAMAELFNQPKILQQAIEELDRIVGKDRLVQESDIPQLKYLIACIR 180
           + + TI NP+N VEWAM EL NQP++L +A EELDR+VGKDRLVQESDIP L Y+ AC R
Sbjct: 335 IMIATIDNPANAVEWAMGELINQPELLAKATEELDRVVGKDRLVQESDIPNLNYVKACAR 394

Query: 181 ESFRLHTFSPFNVPHVSNSDAIVAGYFIPKGSQVLLSRLGLGRNPRIWDDPLSYNPQRHL 240
           E+FRLH  + FNVPHV+  DA++  YFIPKGS  +LSR GLGRNP+ W DPL Y+P+RHL
Sbjct: 395 EAFRLHPVAYFNVPHVAMEDAVIGDYFIPKGSWAILSRYGLGRNPKTWPDPLKYDPERHL 454

Query: 241 NDETVELGLSEPSLRFISFSRGRRGCIGSSLGTTITMMLFARLLQGFSWSLMPGTTKVNF 300
           N+   E+ L+E  LRF++FS GRRGC+ + LGTT+  M+ AR+LQ F+W+  P  T+++ 
Sbjct: 455 NEG--EVVLTEHDLRFVTFSTGRRGCVAALLGTTMITMMLARMLQCFTWTPPPNVTRIDL 514

Query: 301 S-HFDELALPNPLYLYVKPRLSHNMYPS 324
           S + DEL    P+  + KPRL+ ++YP+
Sbjct: 515 SENIDELTPATPITGFAKPRLAPHLYPT 539

[Close]

BLAST of Spg022312 vs. ExPASy TrEMBL
Match: A0A6J1H7W7 (isoleucine N-monooxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111461341 PE=3 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 3.0e-134
Identity = 241/327 (73.70%), Postives = 279/327 (85.32%), Query Frame = 0


Query: 1   MLFDRRYY----NDGGPTFEEEEEHIQALLTLLEHVHAFSVSDYVPCLKLFDLNGHKKTI 60
           MLF++RYY     DGGPTF EEEEH QA LT++ H+HAFS+SD++  LK FDL+GH+K +
Sbjct: 196 MLFNQRYYGKWREDGGPTF-EEEEHDQASLTIIRHLHAFSISDFMSWLKPFDLDGHEKVM 255

Query: 61  KQAFEVIKKHDDSIIDERVQQWKDEKRKEV-DDILDIFISLTDENGKSLLSIQEIKAKIM 120
           K A  V++K D+ IIDERVQ+WKDEK +EV DDILDI ISL DEN K LLS+ EIKA+IM
Sbjct: 256 KSALNVVQKFDEPIIDERVQRWKDEKLEEVKDDILDILISLKDENAKPLLSVVEIKAQIM 315

Query: 121 ELQMTTIGNPSNGVEWAMAELFNQPKILQQAIEELDRIVGKDRLVQESDIPQLKYLIACI 180
           ELQ+ TI NPSN VEWAMAEL NQPKIL++AIEELD++VG++RLVQE DIP LKYL ACI
Sbjct: 316 ELQIATIDNPSNAVEWAMAELLNQPKILEKAIEELDKVVGRERLVQEYDIPNLKYLTACI 375

Query: 181 RESFRLHTFSPFNVPHVSNSDAIVAGYFIPKGSQVLLSRLGLGRNPRIWDDPLSYNPQRH 240
           RESFRLH FSPFNVPHVSN+D IVAGYFIPKGS+VLLSR GLGRNPRIW+DP+ +N +RH
Sbjct: 376 RESFRLHPFSPFNVPHVSNTDIIVAGYFIPKGSEVLLSRTGLGRNPRIWEDPMRFNRERH 435

Query: 241 LNDETVELGLSEPSLRFISFSRGRRGCIGSSLGTTITMMLFARLLQGFSWSLMPGTTKVN 300
           LND TVELGL EP+LRF+SFSRGRRGC GSSLGT ITMMLFARLLQGFSWSL+PG TK++
Sbjct: 436 LNDRTVELGLLEPNLRFMSFSRGRRGCPGSSLGTNITMMLFARLLQGFSWSLLPGITKID 495

Query: 301 FSHFDELALPNPLYLYVKPRLSHNMYP 323
           FS  DEL+LP PL+L  KPRLSH MYP
Sbjct: 496 FSQADELSLPKPLHLLAKPRLSHVMYP 521

[Close]

BLAST of Spg022312 vs. ExPASy TrEMBL
Match: A0A6J1D6G9 (isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017498 PE=3 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 5.1e-134
Identity = 235/327 (71.87%), Postives = 280/327 (85.63%), Query Frame = 0


Query: 1   MLFDRRYY----NDGGPTFEEEEEHIQALLTLLEHVHAFSVSDYVPCLKLFDLNGHKKTI 60
           MLF++RYY     DGGP+F EEEEH QALLT+L HV+AFS+SD++PCLK FDL+GH+K +
Sbjct: 215 MLFNKRYYGKGREDGGPSF-EEEEHNQALLTILRHVNAFSISDFMPCLKPFDLDGHEKIM 274

Query: 61  KQAFEVIKKHDDSIIDERVQQWKDEKRKEVDDILDIFISLTDENGKSLLSIQEIKAKIME 120
           K+A + + K+D+ II+ERVQQWKD KR+  +DILDI IS  D NGKSLL+I EIKA+I E
Sbjct: 275 KRALKALWKYDEPIINERVQQWKDGKREGAEDILDILISTKDGNGKSLLTIDEIKAQITE 334

Query: 121 LQMTTIGNPSNGVEWAMAELFNQPKILQQAIEELDRIVGKDRLVQESDIPQLKYLIACIR 180
           LQ+ TI NPSN VEW MAEL NQPKILQ+AIEELDR+VG++RLVQESDIP+LKYL AC R
Sbjct: 335 LQIATIDNPSNAVEWTMAELLNQPKILQKAIEELDRVVGRERLVQESDIPKLKYLTACAR 394

Query: 181 ESFRLHTFSPFNVPHVSNSDAIVAGYFIPKGSQVLLSRLGLGRNPRIWDDPLSYNPQRHL 240
           ESFRLH FSPFN+PHVSN+D +VAGYFIPKGS+V+LSR GLGRNPRIW+DP+ ++P+RHL
Sbjct: 395 ESFRLHPFSPFNIPHVSNADIVVAGYFIPKGSEVILSRSGLGRNPRIWEDPMRFDPERHL 454

Query: 241 NDETVELGLSEPSLRFISFSRGRRGCIGSSLGTTITMMLFARLLQGFSWSLMPGTTKVNF 300
            DETVELGLSEP+LRFISF+RGRRGC+GSSLGT ITMML  RLLQGFSWSL  GTTK+ F
Sbjct: 455 KDETVELGLSEPNLRFISFTRGRRGCVGSSLGTNITMMLLGRLLQGFSWSLPFGTTKIEF 514

Query: 301 SHFDELALPNPLYLYVKPRLSHNMYPS 324
           S  DEL+LP PL+L+ KPRL H MYP+
Sbjct: 515 SEADELSLPKPLHLHAKPRLPHIMYPT 540

[Close]

BLAST of Spg022312 vs. ExPASy TrEMBL
Match: A0A6J1D470 (isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017448 PE=3 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 1.5e-133
Identity = 238/325 (73.23%), Postives = 278/325 (85.54%), Query Frame = 0


Query: 1   MLFDRRYY----NDGGPTFEEEEEHIQALLTLLEHVHAFSVSDYVPCLKLFDLNGHKKTI 60
           MLF+RRYY     DGGPT  EEEEH QALLT+L HV+AFS+ D++PCLK FDL+GH+KT+
Sbjct: 202 MLFNRRYYGKGREDGGPT-TEEEEHNQALLTILRHVNAFSILDFMPCLKPFDLDGHEKTM 261

Query: 61  KQAFEVIKKHDDSIIDERVQQWKDEKRKEVDDILDIFISLTDENGKSLLSIQEIKAKIME 120
           K+A EV++K+D+ II++RV QWKD KR+ V+DILDI ISL DEN + LLSI+EIKA+ ME
Sbjct: 262 KKALEVLRKYDEPIINKRVHQWKDGKREGVEDILDILISLQDENKRMLLSIEEIKAQTME 321

Query: 121 LQMTTIGNPSNGVEWAMAELFNQPKILQQAIEELDRIVGKDRLVQESDIPQLKYLIACIR 180
           LQ+  I NPSN VEW +AEL NQ KILQ+ IEE+DRIVG+DRLVQESDIP+LKYL AC+R
Sbjct: 322 LQLAMIDNPSNAVEWTIAELLNQSKILQKVIEEMDRIVGRDRLVQESDIPKLKYLTACVR 381

Query: 181 ESFRLHTFSPFNVPHVSNSDAIVAGYFIPKGSQVLLSRLGLGRNPRIWDDPLSYNPQRHL 240
           ESFRLH FSPFNVPHVSNSD IVAGYFIPKGS+V+LSRLGLGRN RIW DP+ ++P+RHL
Sbjct: 382 ESFRLHPFSPFNVPHVSNSDIIVAGYFIPKGSEVVLSRLGLGRNTRIWKDPMRFDPERHL 441

Query: 241 NDETVELGLSEPSLRFISFSRGRRGCIGSSLGTTITMMLFARLLQGFSWSLMPGTTKVNF 300
            D TVE GLSEPSLRFISFSRGRRGC+GSSLGT+ITMMLFARLLQGFSW+L+PGTTKV F
Sbjct: 442 KDGTVEFGLSEPSLRFISFSRGRRGCVGSSLGTSITMMLFARLLQGFSWNLLPGTTKVGF 501

Query: 301 SHFDELALPNPLYLYVKPRLSHNMY 322
           S  DEL L  PL+L+ KPRL H MY
Sbjct: 502 SESDELCLSKPLHLHAKPRLFHIMY 525

[Close]

BLAST of Spg022312 vs. ExPASy TrEMBL
Match: A0A6J1DNF2 (isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111021625 PE=3 SV=1)

HSP 1 Score: 485.0 bits (1247), Expect = 2.5e-133
Identity = 232/325 (71.38%), Postives = 278/325 (85.54%), Query Frame = 0


Query: 1   MLFDRRYY----NDGGPTFEEEEEHIQALLTLLEHVHAFSVSDYVPCLKLFDLNGHKKTI 60
           M+F+ RYY     DGGPTF EEEEH QALLT+L H+HAFS+SDY+PCL+  DL+GH+K +
Sbjct: 213 MIFNTRYYGGGREDGGPTF-EEEEHTQALLTILNHIHAFSISDYMPCLRTLDLDGHQKIV 272

Query: 61  KQAFEVIKKHDDSIIDERVQQWKDEKRKEVDDILDIFISLTDENGKSLLSIQEIKAKIME 120
           K A +VI+K+D+ II+ERV++WK    KEV DILDI ISL DENGKSLLSI+EIK +IME
Sbjct: 273 KNALKVIRKYDEPIINERVERWKHGNNKEVVDILDILISLPDENGKSLLSIEEIKTQIME 332

Query: 121 LQMTTIGNPSNGVEWAMAELFNQPKILQQAIEELDRIVGKDRLVQESDIPQLKYLIACIR 180
           LQ+ T+ NPSN VEWAMAEL NQPKIL++A+EELDR VG++RLV+E DIP+LKYL AC+R
Sbjct: 333 LQVATVDNPSNAVEWAMAELINQPKILKKAVEELDREVGRERLVEEYDIPKLKYLTACVR 392

Query: 181 ESFRLHTFSPFNVPHVSNSDAIVAGYFIPKGSQVLLSRLGLGRNPRIWDDPLSYNPQRHL 240
           ESFRLH FSPFN+PHVSNSD +VAGY IPKGS+VLLSR GLGRNPRIW+DP+ ++P+RHL
Sbjct: 393 ESFRLHPFSPFNLPHVSNSDIVVAGYLIPKGSEVLLSRSGLGRNPRIWEDPMRFDPERHL 452

Query: 241 NDETVELGLSEPSLRFISFSRGRRGCIGSSLGTTITMMLFARLLQGFSWSLMPGTTKVNF 300
            D+TVELGLSEPSLRFI+F+RGRRGCIGSSLGTTITMMLFAR+LQ FSWSL PG T ++F
Sbjct: 453 KDKTVELGLSEPSLRFITFTRGRRGCIGSSLGTTITMMLFARMLQAFSWSLPPGMTMIDF 512

Query: 301 SHFDELALPNPLYLYVKPRLSHNMY 322
           S  DEL+LP PLYL+ + RLS  MY
Sbjct: 513 SESDELSLPKPLYLFAEARLSSTMY 536

[Close]

BLAST of Spg022312 vs. ExPASy TrEMBL
Match: A0A6J1DJC5 (isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111021624 PE=3 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 4.3e-133
Identity = 238/327 (72.78%), Postives = 281/327 (85.93%), Query Frame = 0


Query: 2   LFDRRYY----NDGGPTFEEEEEHIQALLTLLEHVHAFSVSDYVPCLKLFDLNGHKKTIK 61
           LF+ RYY     DGGP+F EEEEH QALLT+L+H++AFS+SD++PCLK FDL+GH K +K
Sbjct: 179 LFNTRYYGKGSEDGGPSF-EEEEHNQALLTILKHINAFSISDFMPCLKPFDLDGHGKIMK 238

Query: 62  QAFEVIKKHDDSIIDERVQQWKDEKRKEVDDILDIFISLTDENGKSLLSIQEIKAKIMEL 121
           QA EV++K+D+ II+ERVQQWKD KR+ V+DILDI ISL DE+G+SLLSI EIKA+I EL
Sbjct: 239 QALEVLRKYDEPIINERVQQWKDGKREGVEDILDILISLKDESGESLLSIDEIKAQITEL 298

Query: 122 QMTTIGNPSNGVEWAMAELFNQPKILQQAIEELDRIVGKDRLVQESDIPQLKYLIACIRE 181
           Q+ TI NPSN VEW MAEL NQPKILQ+A+EELDR+VG+DRLVQESDIP+LKYL AC RE
Sbjct: 299 QIATIDNPSNTVEWTMAELLNQPKILQKAVEELDRVVGRDRLVQESDIPKLKYLTACARE 358

Query: 182 SFRLHTFSPFNVPHVSNSDAIVAGYFIPKGSQVLLSRLGLGRNPRIWDDPLSYNPQRHLN 241
           S RLH FSPFN+PHVSNSD IVAG+FIPKGS+V+LSR GLGRNPRIW++P  ++P+RHL 
Sbjct: 359 SLRLHPFSPFNIPHVSNSDVIVAGFFIPKGSEVILSRPGLGRNPRIWENPNRFDPERHLT 418

Query: 242 DE-TVELGLSEPSLRFISFSRGRRGCIGSSLGTTITMMLFARLLQGFSWSLMPGTTKVNF 301
           DE T+ELGLSEPSLRFISF+RGRRGCIGSSLGT ITMMLFARLLQGFSWSL  G TK+ F
Sbjct: 419 DESTIELGLSEPSLRFISFTRGRRGCIGSSLGTNITMMLFARLLQGFSWSLPLGMTKIEF 478

Query: 302 SHFDELALPNPLYLYVKPRLSHNMYPS 324
           S  DEL+ P PL+L+ KPRL H MYP+
Sbjct: 479 SEADELSFPKPLHLHAKPRLPHIMYPT 504

[Close]

BLAST of Spg022312 vs. TAIR 10
Match: AT5G05260.1 (cytochrome p450 79a2 )

HSP 1 Score: 351.7 bits (901), Expect = 6.4e-97
Identity = 162/328 (49.39%), Postives = 233/328 (71.04%), Query Frame = 0


Query: 1   MLFDRRYY----NDGGPTFEEEEEHIQALLTLLEHVHAFSVSDYVPCLKLFDLNGHKKTI 60
           M+F  R++     DG     EE EH+++L T+L H++AF++SDYVP L+  DL GH+K +
Sbjct: 197 MMFGIRHFGKGSEDGSGPGLEEIEHVESLFTVLTHLYAFALSDYVPWLRFLDLEGHEKVV 256

Query: 61  KQAFEVIKKHDDSIIDERVQQWKDEKRKEVDDILDIFISLTDENGKSLLSIQEIKAKIME 120
             A   + K++D  +DER+ QW++ K KE  D LD+FI   D +GK  LS +EIKA++ E
Sbjct: 257 SNAMRNVSKYNDPFVDERLMQWRNGKMKEPQDFLDMFIIAKDTDGKPTLSDEEIKAQVTE 316

Query: 121 LQMTTIGNPSNGVEWAMAELFNQPKILQQAIEELDRIVGKDRLVQESDIPQLKYLIACIR 180
           L + T+ NPSN  EW MAE+ N+P I+Q+A+EE+DR+VGKDRLV ESD+P L Y+ AC++
Sbjct: 317 LMLATVDNPSNAAEWGMAEMINEPSIMQKAVEEIDRVVGKDRLVIESDLPNLNYVKACVK 376

Query: 181 ESFRLHTFSPFNVPHVSNSDAIVAGYFIPKGSQVLLSRLGLGRNPRIWDDPLSYNPQRHL 240
           E+FRLH  +PFN+PH+S +D +V GYFIPKGS VL+SR+G+GRNP +WD P  ++P+RHL
Sbjct: 377 EAFRLHPVAPFNLPHMSTTDTVVDGYFIPKGSHVLISRMGIGRNPSVWDKPHKFDPERHL 436

Query: 241 NDETVELGLSEPSLRFISFSRGRRGCIGSSLGTTITMMLFARLLQGFSWSLMPGTTKVNF 300
           +  T  + L+E  L  ISFS GRRGC+G  +G+ +T ML ARL+QGF+W  +PG  K++ 
Sbjct: 437 STNTC-VDLNESDLNIISFSAGRRGCMGVDIGSAMTYMLLARLIQGFTWLPVPGKNKIDI 496

Query: 301 SHF-DELALPNPLYLYVKPRLSHNMYPS 324
           S   ++L +  PLY    PRL+ ++YP+
Sbjct: 497 SESKNDLFMAKPLYAVATPRLAPHVYPT 523

[Close]

BLAST of Spg022312 vs. TAIR 10
Match: AT2G22330.1 (cytochrome P450, family 79, subfamily B, polypeptide 3 )

HSP 1 Score: 342.8 bits (878), Expect = 3.0e-94
Identity = 172/321 (53.58%), Postives = 226/321 (70.40%), Query Frame = 0


Query: 3   FDRRYYNDGGPTFEEEEEHIQALLTLLEHVHAFSVSDYVPCLKLFDLNGHKKTIKQAFEV 62
           F  +   DGGPT  E+ EH+ A+   L    AF +SDY+P L   DLNGH+K ++++  +
Sbjct: 222 FSEKTEADGGPTL-EDIEHMDAMFEGLGFTFAFCISDYLPMLTGLDLNGHEKIMRESSAI 281

Query: 63  IKKHDDSIIDERVQQWKDEKRKEVDDILDIFISLTDENGKSLLSIQEIKAKIMELQMTTI 122
           + K+ D IIDER++ W++ KR +++D LDIFIS+ DE G+ LL+  EIK  I EL M   
Sbjct: 282 MDKYHDPIIDERIKMWREGKRTQIEDFLDIFISIKDEAGQPLLTADEIKPTIKELVMAAP 341

Query: 123 GNPSNGVEWAMAELFNQPKILQQAIEELDRIVGKDRLVQESDIPQLKYLIACIRESFRLH 182
            NPSN VEWA+AE+ N+P+IL +A+EE+DR+VGK+R VQESDIP+L Y+ A IRE+FRLH
Sbjct: 342 DNPSNAVEWAIAEMINKPEILHKAMEEIDRVVGKERFVQESDIPKLNYVKAIIREAFRLH 401

Query: 183 TFSPFNVPHVSNSDAIVAGYFIPKGSQVLLSRLGLGRNPRIWDDPLSYNPQRHLNDETVE 242
             + FN+PHV+ SD  VAGY IPKGSQVLLSR GLGRNP++W DPLS+ P+RHLN E  E
Sbjct: 402 PVAAFNLPHVALSDTTVAGYHIPKGSQVLLSRYGLGRNPKVWSDPLSFKPERHLN-ECSE 461

Query: 243 LGLSEPSLRFISFSRGRRGCIGSSLGTTITMMLFARLLQGFSWSLMPGTTKVNFSHFD-E 302
           + L+E  LRFISFS G+RGC   +LGT IT M+ ARLLQGF W L    T+V       +
Sbjct: 462 VTLTENDLRFISFSTGKRGCAAPALGTAITTMMLARLLQGFKWKLAGSETRVELMESSHD 521

Query: 303 LALPNPLYLYVKPRLSHNMYP 323
           + L  PL L  + RLS ++YP
Sbjct: 522 MFLSKPLVLVGELRLSEDLYP 540

[Close]

BLAST of Spg022312 vs. TAIR 10
Match: AT4G39950.1 (cytochrome P450, family 79, subfamily B, polypeptide 2 )

HSP 1 Score: 341.3 bits (874), Expect = 8.6e-94
Identity = 169/322 (52.48%), Postives = 227/322 (70.50%), Query Frame = 0


Query: 3   FDRRYYNDGGPTFEEEEEHIQALLTLLEHVHAFSVSDYVPCLKLFDLNGHKKTIKQAFEV 62
           F +    DGGPT  E+ EH++A+   L    AF +SDY+P L   DLNGH+K ++++  +
Sbjct: 220 FSKNTAPDGGPTV-EDVEHMEAMFEALGFTFAFCISDYLPMLTGLDLNGHEKIMRESSAI 279

Query: 63  IKKHDDSIIDERVQQWKDEKRKEVDDILDIFISLTDENGKSLLSIQEIKAKIMELQMTTI 122
           + K+ D IIDER++ W++ KR +++D LDIFIS+ DE G  LL+  EIK  I EL M   
Sbjct: 280 MDKYHDPIIDERIKMWREGKRTQIEDFLDIFISIKDEQGNPLLTADEIKPTIKELVMAAP 339

Query: 123 GNPSNGVEWAMAELFNQPKILQQAIEELDRIVGKDRLVQESDIPQLKYLIACIRESFRLH 182
            NPSN VEWAMAE+ N+P+IL++A+EE+DR+VGK+RLVQESDIP+L Y+ A +RE+FRLH
Sbjct: 340 DNPSNAVEWAMAEMVNKPEILRKAMEEIDRVVGKERLVQESDIPKLNYVKAILREAFRLH 399

Query: 183 TFSPFNVPHVSNSDAIVAGYFIPKGSQVLLSRLGLGRNPRIWDDPLSYNPQRHLNDETVE 242
             + FN+PHV+ SD  VAGY IPKGSQVLLSR GLGRNP++W DPL + P+RHLN E  E
Sbjct: 400 PVAAFNLPHVALSDTTVAGYHIPKGSQVLLSRYGLGRNPKVWADPLCFKPERHLN-ECSE 459

Query: 243 LGLSEPSLRFISFSRGRRGCIGSSLGTTITMMLFARLLQGFSWSLMPGTTKVNFSHFD-E 302
           + L+E  LRFISFS G+RGC   +LGT +T M+ ARLLQGF+W L    T+V       +
Sbjct: 460 VTLTENDLRFISFSTGKRGCAAPALGTALTTMMLARLLQGFTWKLPENETRVELMESSHD 519

Query: 303 LALPNPLYLYVKPRLSHNMYPS 324
           + L  PL +    RL  ++YP+
Sbjct: 520 MFLAKPLVMVGDLRLPEHLYPT 539

[Close]

BLAST of Spg022312 vs. TAIR 10
Match: AT1G79370.1 (cytochrome P450, family 79, subfamily C, polypeptide 1 )

HSP 1 Score: 302.0 bits (772), Expect = 5.8e-82
Identity = 156/338 (46.15%), Postives = 221/338 (65.38%), Query Frame = 0


Query: 1   MLFDRRYYND---GGPTFEEEEEHIQALLTLLEHVHAFSVSDYVPCLKLFDLNGHKKTIK 60
           +LF RR++ +    G     E+EH  A+   L+   +F V+DY P L+ ++L G +  ++
Sbjct: 204 LLFGRRHFKETTMDGSLGLMEKEHFDAIFAALDCFFSFYVADYYPFLRGWNLQGEEAELR 263

Query: 61  QAFEVIKKHDDSIIDERVQQW----KDEKRKE-------VDDILDIFISLTDENGKSLLS 120
           +A +VI +++  IIDE+++ W    KD  R E       + D LDI  +L DENGK LL+
Sbjct: 264 EAVDVIARYNKMIIDEKIELWRGQNKDYNRAETKNDVPMIKDWLDILFTLKDENGKPLLT 323

Query: 121 IQEIKAKIMELQMTTIGNPSNGVEWAMAELFNQPKILQQAIEELDRIVGKDRLVQESDIP 180
            QEI    ++L +  I N  N +EW +AE+ NQ +IL++A+EE+D +VGK+RLVQESD+P
Sbjct: 324 PQEITHLSVDLDVVGIDNAVNVIEWTLAEMLNQREILEKAVEEIDMVVGKERLVQESDVP 383

Query: 181 QLKYLIACIRESFRLHTFSPFNVPHVSNSDAIVAGYFIPKGSQVLLSRLGLGRNPRIWDD 240
            L Y+ AC RE+ RLH  +PF VPH++  D  +AGYFIPKGS +L+SR G+GRNP+ WD+
Sbjct: 384 NLNYVKACCRETLRLHPTNPFLVPHMARHDTTLAGYFIPKGSHILVSRPGVGRNPKTWDE 443

Query: 241 PLSYNPQRHLNDETVELGLSEPSLRFISFSRGRRGCIGSSLGTTITMMLFARLLQGFSWS 300
           PL Y P+RH+     E+ L+EP LR +SF  GRRGC+G+ LGT++ + L  RLLQGF W+
Sbjct: 444 PLIYRPERHITGN--EVVLTEPDLRLVSFGTGRRGCVGAKLGTSMIVTLLGRLLQGFDWT 503

Query: 301 LMPGTT-KVNFSHFDE-LALPNPLYLYVKPRLSHNMYP 323
           + PGTT +V      E L + NPL   VKPRL  NMYP
Sbjct: 504 IPPGTTDRVELVESKENLFMANPLMACVKPRLDPNMYP 539

[Close]

BLAST of Spg022312 vs. TAIR 10
Match: AT1G16410.1 (cytochrome p450 79f1 )

HSP 1 Score: 291.2 bits (744), Expect = 1.0e-78
Identity = 148/333 (44.44%), Postives = 219/333 (65.77%), Query Frame = 0


Query: 1   MLFDRRY------YNDGGPTFEEEEEHIQALLTLLEHVHAFSVSDYVP-CLKLFDLNGHK 60
           MLF RR+      ++D G     E+ H++ +   L  + +FS +DYV   L+ ++++G +
Sbjct: 202 MLFGRRHVTKENVFSDDGRLGNAEKHHLEVIFNTLNCLPSFSPADYVERWLRGWNVDGQE 261

Query: 61  KTIKQAFEVIKKHDDSIIDERVQQWKDEKRK-EVDDILDIFISLTDENGKSLLSIQEIKA 120
           K + +   +++ +++ IIDERVQ W++E  K  V+D LD FI+L D+NGK L++  EIKA
Sbjct: 262 KRVTENCNIVRSYNNPIIDERVQLWREEGGKAAVEDWLDTFITLKDQNGKYLVTPDEIKA 321

Query: 121 KIMELQMTTIGNPSNGVEWAMAELFNQPKILQQAIEELDRIVGKDRLVQESDIPQLKYLI 180
           + +E  +  I NP+N +EW + E+   P+IL++A++ELD +VG+DRLVQESDIP L YL 
Sbjct: 322 QCVEFCIAAIDNPANNMEWTLGEMLKNPEILRKALKELDEVVGRDRLVQESDIPNLNYLK 381

Query: 181 ACIRESFRLHTFSPFNVPHVSNSDAIVAGYFIPKGSQVLLSRLGLGRNPRIWDDPLSYNP 240
           AC RE+FR+H  + +   H++  D  + GYFIPKGS + + R GLGRNP+IW DPL Y P
Sbjct: 382 ACCRETFRIHPSAHYVPSHLARQDTTLGGYFIPKGSHIHVCRPGLGRNPKIWKDPLVYKP 441

Query: 241 QRHLNDE--TVELGLSEPSLRFISFSRGRRGCIGSSLGTTITMMLFARLLQGFSWSLMPG 300
           +RHL  +  T E+ L E  +RF+SFS GRRGCIG  +GT + +ML AR LQGF+W L   
Sbjct: 442 ERHLQGDGITKEVTLVETEMRFVSFSTGRRGCIGVKVGTIMMVMLLARFLQGFNWKLHQD 501

Query: 301 TTKVNFSHFD-ELALPNPLYLYVKPRLSHNMYP 323
              ++    D  L +  PL+L V+PRL+ N+YP
Sbjct: 502 FGPLSLEEDDASLLMAKPLHLSVEPRLAPNLYP 534

The following BLAST results are available for this feature:

BLAST of Spg022312 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023516709.1	9.5e-135	73.09	isoleucine N-monooxygenase 2-like [Cucurbita pepo subsp. pepo]	[more]
XP_038878794.1	3.6e-134	71.12	tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida]	[more]
XP_038880438.1	6.1e-134	70.34	tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida]	[more]
XP_022960607.1	6.1e-134	73.70	isoleucine N-monooxygenase 1-like [Cucurbita moschata]	[more]
KAG6590174.1	1.0e-133	73.09	Isoleucine N-monooxygenase 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Spg022312 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A068Q605	5.8e-103	55.89	Tryptophan N-monooxygenase CYP79A68 OS=Prunus mume OX=102107 GN=CYP79A68 PE=1 SV...	[more]
Q9FLC8	9.0e-96	49.39	Phenylalanine N-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP79A2 PE=1 SV...	[more]
Q6J541	2.0e-95	51.53	Isoleucine N-monooxygenase 1 OS=Lotus japonicus OX=34305 GN=CYP79D3 PE=1 SV=1	[more]
Q43135	1.7e-94	50.61	Tyrosine N-monooxygenase OS=Sorghum bicolor OX=4558 GN=CYP79A1 PE=1 SV=3	[more]
Q9M7B7	2.2e-94	50.91	Valine N-monooxygenase 2 OS=Manihot esculenta OX=3983 GN=CYP79D2 PE=1 SV=1	[more]

BLAST of Spg022312 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H7W7	3.0e-134	73.70	isoleucine N-monooxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111461341 ...	[more]
A0A6J1D6G9	5.1e-134	71.87	isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017498...	[more]
A0A6J1D470	1.5e-133	73.23	isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017448...	[more]
A0A6J1DNF2	2.5e-133	71.38	isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111021625...	[more]
A0A6J1DJC5	4.3e-133	72.78	isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111021624...	[more]

BLAST of Spg022312 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G05260.1	6.4e-97	49.39	cytochrome p450 79a2	[more]
AT2G22330.1	3.0e-94	53.58	cytochrome P450, family 79, subfamily B, polypeptide 3	[more]
AT4G39950.1	8.6e-94	52.48	cytochrome P450, family 79, subfamily B, polypeptide 2	[more]
AT1G79370.1	5.8e-82	46.15	cytochrome P450, family 79, subfamily C, polypeptide 1	[more]
AT1G16410.1	1.0e-78	44.44	cytochrome p450 79f1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 213..237 score: 24.4 coord: 252..262 score: 49.29 coord: 129..155 score: 30.3 coord: 172..190 score: 36.36 coord: 262..285 score: 29.91
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 14..309 e-value: 1.4E-62 score: 212.0
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..320 e-value: 3.2E-65 score: 222.5
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 16..315
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 1..320
None	No IPR available	PANTHER	PTHR24298:SF675	PHENYLALANINE N-MONOOXYGENASE	coord: 1..320
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 255..264

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg022312.1	Spg022312.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen