Spg022161 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg022161
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionNAD(P)-binding Rossmann-fold superfamily protein
Locationscaffold2: 7410094 .. 7414117 (+)
RNA-Seq ExpressionSpg022161
SyntenySpg022161
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTGCACTCACGAAGCTTCACGTGTACCCACAAAGTTTCGATGGCCCGAGTACCAGAGATGGGCACCTGATCGGAGCTCTTGGACGGGGAGTCCACCGGTTTGGCGTTGGAGTGTCGACGAGCCGTGGTGGGTTGTGCTTGAGAAAGTGTAGATCCTTCAGAGGCGAGGATGGTGGGGATTTTGACAAGGAGGATGGTAAAGGGAGGAATCGTGGAAAATCGAGGTTAAAAGAGGTGAAAGTGAAGAAGGAAAGTCAGTTTTGGAAATTTTTGAGGTCTGGTGTTTTGGGTAAGTTTAATTTGCTTTTGGGGTCGGATGTTGAACGGGGGAAGCTTGTGGCAAATATGGAGGGTTTGTTTTCTTCGGTGAGTGTTACTGGATACTGGTCTTCCTATCTTCTTCTTAGACTTTGTTTGAATTTAATTTATTCTCTGTTCATAAGTGACGTTGAGTTGTTTAGAAAGAATTTGAAGTGTTTTTTGGTTAATCCCCCGGTGTTTGTTTCGTCATCATCAGTTGACTTGTTGAGCAAATGTGCCTGCAGTTATAGCTTTCACGACTGTAACTTGAAGTTTCATCATCAACTGGAAGTTTTTCCTTCGTTGAGTTATTTGTTGTGAGGAATTCGAATTTTAATTTTGGATAATTGGAAATTATAACGATATGAATAAACAAGGAGGATTCTAAGCTAAGCAGCTATGGCATGAAATTGAACCAAAGCCAACTTAAACTGTAGATAAATAGATCTATTTTGTTCTATGGTAGTTTTGATGTATATTCATGGATCAAGGAGGACATGTGCTTGATCTTCATTTGGAGACTGTTGTAGCATCACTAACTGTAGCTCCTTTATTTTCTTAATAGGCTGCAGTCCAAATTGGAAGATATATTGTTACAATGATGAGCACTGGTGTCATACTTGCTGTCGGGTTTCAGTTATCAGGTATCCCACATTTTAAATGTTTTATTTTAAAAAAATTACAAACGTCTCATTAACATTGTCAATGTTAATATGAATTCTGTTTCTTGAAAATCATGAAGGTGGAGACAGTCAGATGAACACACTGATTTGGTATAGTTGGCTTGGAGGGGTTATTATTGGAACAATGATTGGTGCTAACATGGTATTAGAGGAGCACTGTAGAGCTGGTCCTCGCAATGTCGTTATAACTGGAAGGTAATTAGTCACTCCTCATGAAGAATGTAGTTCATTGGCACGACCAACTGGTCTTTGTTTTAATGCATTTCATGGTGATAGGATGGATTACTTCGTTTAATTTTTATTGTTTACTTGTATGGTATTCTTATATCATGTGATTTAAACTGCCTACTACGTTCATAACATGAAAGATTTGTACATGATGGTACATGGATGGTTGACTATGCAGCACAAGAGGATTGGGGAAAGCGCTAGCCAGAGAGTTTCTTCTCTCGGGAGACCGGGTGGTTGTGGCTTCTCGCAGGTGGTATTCAGGATCTATATAAAACTTCTGATACACAGTATCTCAACATTTTTACTACTTGCTTACATCACATTTCCTTGCCCAATGATTTTGTGAAGACAGCCCCGAGTCTGTTCAAGAAACTCTCAGAGAGCTCGAGGAAAACTTAAAAGGTCTAATCGCCAGAAGTGGTTCTTCCAATACAAGCCTGGCACATGCTAAAGTTGTTGGTACAACGTGCAATGTTTGCGACCCTGAAGATGTTCATAATTTAGCAAATTTTGCTTTGAATGAACTTGGTTCCATTGACATTTGGGTGAGTCATGAACTTACAAAGTGGATTTTTCATGCACAAACTGGGATAATATGCTGACTTTTCTTGAAATTTTTTCTAATGATATTGAGATTTTTGATCGAATGACACATTCTTAAAGTAGCTGCCTCCATGCTTCAATGCCTTCCAGGGAAAAAAATGGATCTATCTGACCATCAGAAAACAAGAGATCCTTATTCAAAAACATTAATATTTTAGATCATCAAGGATAGAAGAGTCATGTCTCTAGGCGATAGCTTATCTCATTGAGAAGGAAAAAATCTTGAATTTTTTTAACGTGACAAGTTATGGGCAGATAAACAATGCGGGCACGAATAAAGGCTTTAGACCCTTACTGCAGTTCACGGATGAAGATATTACTCAGGTAAAGTTATACAGTAATTCTGGATCTTTATTCCTATACTTCTCTTGATAAATCTGAACATAAACTTGTCCAATATATATGAGCTATGTAAGAAGGTGTTTAGTGGGATGTCGGTTTATCGCATATTTGCAATATCCCAGGCTCTGACTGTTACTAATTTCTGAAAATAAATGTAATTGGAGTCTCACTGAAATTTGAATGTTGACGGTTTAAAACTAAAGCACTGAAACTGGACTTTCACTTTGTGTATGAATGGAGGGCATGTTTTACCACACTCAACTAACGTCCTGGTTGAAATTTCATGTTGTCCTTTTTGATGTCTCAACTCTGTTTATGCCTTTATGGTGCCTTTGAAAATTTGATGTTATAACGTATTAGTTGATTTAATTTCATACACTGGATATTTGATAGATTCTCTCAACAAACCTCGTTGGTTCCCTTCTTTGTACTCGAGAAGCCATGCGTGTAATGAGAGATCAGGCTAAAGGAGGGCACATATTTAACATGGACGGTGCTGGATCTGGAGGGTCAAGTACTCCTTTGACGGCTGTGTAAGTTCATTGCATACTGTGAATGTTAATGCAGTTTCGTATGGAGTGGAGGGTTTAATTCAAATTCTGGGAATTGTTTTTAACTCGTGAAACTGGTCGTATGCTTGTGTCTATGATATCAACAGTCGGTTTTGGTGTTTACACTATTTAGTTTACTGAACTTGTAGGTATGGATCGACGAAATGCGGTCTTAGACAGCTTCAATCATCTCTTTTGAAAGAGTGCAGGCGGTCCAATGTAGGAGTGCACACTGCATCTCCAGGCATGGTTTTGACAGATCTCCTTCTTAGGTGAGTCAAGTTTCTTCTCATTTTCTTATCAACGTTTTCTGTCTTGCCATCTTCCCATAAATTTCAGAGGTGTGTTATACTTGGTTGATATACATTCTTCTAATAAACATTACCCGTTGGAACAACTCTTACCAAGATTTTGCTATGTGATACTTGTGGCTTGTATACAGTGGTTCAACTGTGAGAAACAAACAGATGTTCAATATCATTTGTGAGCTTCCTGAAACAGTGGCTAGAACATTAGTTCCTCGGATGAGGGTTGTTAAAGGAACAGGAAAAGCCATCAACTACTTGACTCCTCCAAGAATCTTATTAGCTCTGGTCACTGCTTGGCTGCGACGAGGTCGGTGGTTTGACGAACAGGTAAGTTCAATTCCATCGTGTAAAGTAGCAAACACAAATGCTTCAAAAGTTCAATAATTCATATTATTAAAAAAATCATCAAGAACCCAGATGAGCAATTATGGAGGGTTGTTATTCATCTAGTCTGTGATATATTGCAGGGGAAGGCTCTGTATGCAGCCGAGGCAGACAGAATCCGTAACTGGGCCGAAAACCGCACTCGTTTCTCCTTCACTGATGCAATGGAGATGTACACAGAAAACACCTGGGTGTCTGTCTTCTCCCTTTCAGTCGTTTGTGCCTTCATAATTCTCTCAACCACAAACACCACACTTCCCGGAACTTGATTTTCTTCAACTACATTACAAGCCAGACAGCATTCGAAATTCATCGGGAAAGCGTCGTTAACGATGCAGCATTGTTAGGTACTGATAGGGGAGAAAAGAAAGGAGAAAGGGACATTGTTAGTTCACCCTCCATTCTTGCACACTAAGAAAAACCAACCCCCACTTTCCACTAGCAAATGTATTGACTGCCTAACTGCAAAGCCCTGAAGAGGGCCTCTCGAGGCCTATTTATTTATTTAGTTCGATGGACTGATCTGTGTTGAAATGTAGTGTGTAAAAAGTTAACAAGAATTTGAGTAAAGTTTGTGCCTTTTTTAACAGCACTCAACTTTCATCTTTCACTCTGTGTG

mRNA sequence

ATGTCTGCACTCACGAAGCTTCACGTGTACCCACAAAGTTTCGATGGCCCGAGTACCAGAGATGGGCACCTGATCGGAGCTCTTGGACGGGGAGTCCACCGGTTTGGCGTTGGAGTGTCGACGAGCCGTGGTGGGTTGTGCTTGAGAAAGTGTAGATCCTTCAGAGGCGAGGATGGTGGGGATTTTGACAAGGAGGATGGTAAAGGGAGGAATCGTGGAAAATCGAGGTTAAAAGAGGTGAAAGTGAAGAAGGAAAGTCAGTTTTGGAAATTTTTGAGGTCTGGTGTTTTGGGTAAGTTTAATTTGCTTTTGGGGTCGGATGTTGAACGGGGGAAGCTTGTGGCAAATATGGAGGGTTTGTTTTCTTCGGCTGCAGTCCAAATTGGAAGATATATTGTTACAATGATGAGCACTGGTGTCATACTTGCTGTCGGGTTTCAGTTATCAGGTGGAGACAGTCAGATGAACACACTGATTTGGTATAGTTGGCTTGGAGGGGTTATTATTGGAACAATGATTGGTGCTAACATGGTATTAGAGGAGCACTGTAGAGCTGGTCCTCGCAATGTCGTTATAACTGGAAGCACAAGAGGATTGGGGAAAGCGCTAGCCAGAGAGTTTCTTCTCTCGGGAGACCGGGTGGTTGTGGCTTCTCGCAGCCCCGAGTCTGTTCAAGAAACTCTCAGAGAGCTCGAGGAAAACTTAAAAGGTCTAATCGCCAGAAGTGGTTCTTCCAATACAAGCCTGGCACATGCTAAAGTTGTTGGTACAACGTGCAATGTTTGCGACCCTGAAGATGTTCATAATTTAGCAAATTTTGCTTTGAATGAACTTGGTTCCATTGACATTTGGATAAACAATGCGGGCACGAATAAAGGCTTTAGACCCTTACTGCAGTTCACGGATGAAGATATTACTCAGATTCTCTCAACAAACCTCGTTGGTTCCCTTCTTTGTACTCGAGAAGCCATGCGTGTAATGAGAGATCAGGCTAAAGGAGGGCACATATTTAACATGGACGGTGCTGGATCTGGAGGGTCAAGTACTCCTTTGACGGCTGTGTATGGATCGACGAAATGCGGTCTTAGACAGCTTCAATCATCTCTTTTGAAAGAGTGCAGGCGGTCCAATGTAGGAGTGCACACTGCATCTCCAGGCATGGTTTTGACAGATCTCCTTCTTAGTGGTTCAACTGTGAGAAACAAACAGATGTTCAATATCATTTGTGAGCTTCCTGAAACAGTGGCTAGAACATTAGTTCCTCGGATGAGGGTTGTTAAAGGAACAGGAAAAGCCATCAACTACTTGACTCCTCCAAGAATCTTATTAGCTCTGGTCACTGCTTGGCTGCGACGAGGTCGGTGGTTTGACGAACAGGGGAAGGCTCTGTATGCAGCCGAGGCAGACAGAATCCGTAACTGGGCCGAAAACCGCACTCGTTTCTCCTTCACTGATGCAATGGAGATGTACACAGAAAACACCTGGGTGTCTGTCTTCTCCCTTTCAGTCGTTTGTGCCTTCATAATTCTCTCAACCACAAACACCACACTTCCCGGAACTTGA

Coding sequence (CDS)

ATGTCTGCACTCACGAAGCTTCACGTGTACCCACAAAGTTTCGATGGCCCGAGTACCAGAGATGGGCACCTGATCGGAGCTCTTGGACGGGGAGTCCACCGGTTTGGCGTTGGAGTGTCGACGAGCCGTGGTGGGTTGTGCTTGAGAAAGTGTAGATCCTTCAGAGGCGAGGATGGTGGGGATTTTGACAAGGAGGATGGTAAAGGGAGGAATCGTGGAAAATCGAGGTTAAAAGAGGTGAAAGTGAAGAAGGAAAGTCAGTTTTGGAAATTTTTGAGGTCTGGTGTTTTGGGTAAGTTTAATTTGCTTTTGGGGTCGGATGTTGAACGGGGGAAGCTTGTGGCAAATATGGAGGGTTTGTTTTCTTCGGCTGCAGTCCAAATTGGAAGATATATTGTTACAATGATGAGCACTGGTGTCATACTTGCTGTCGGGTTTCAGTTATCAGGTGGAGACAGTCAGATGAACACACTGATTTGGTATAGTTGGCTTGGAGGGGTTATTATTGGAACAATGATTGGTGCTAACATGGTATTAGAGGAGCACTGTAGAGCTGGTCCTCGCAATGTCGTTATAACTGGAAGCACAAGAGGATTGGGGAAAGCGCTAGCCAGAGAGTTTCTTCTCTCGGGAGACCGGGTGGTTGTGGCTTCTCGCAGCCCCGAGTCTGTTCAAGAAACTCTCAGAGAGCTCGAGGAAAACTTAAAAGGTCTAATCGCCAGAAGTGGTTCTTCCAATACAAGCCTGGCACATGCTAAAGTTGTTGGTACAACGTGCAATGTTTGCGACCCTGAAGATGTTCATAATTTAGCAAATTTTGCTTTGAATGAACTTGGTTCCATTGACATTTGGATAAACAATGCGGGCACGAATAAAGGCTTTAGACCCTTACTGCAGTTCACGGATGAAGATATTACTCAGATTCTCTCAACAAACCTCGTTGGTTCCCTTCTTTGTACTCGAGAAGCCATGCGTGTAATGAGAGATCAGGCTAAAGGAGGGCACATATTTAACATGGACGGTGCTGGATCTGGAGGGTCAAGTACTCCTTTGACGGCTGTGTATGGATCGACGAAATGCGGTCTTAGACAGCTTCAATCATCTCTTTTGAAAGAGTGCAGGCGGTCCAATGTAGGAGTGCACACTGCATCTCCAGGCATGGTTTTGACAGATCTCCTTCTTAGTGGTTCAACTGTGAGAAACAAACAGATGTTCAATATCATTTGTGAGCTTCCTGAAACAGTGGCTAGAACATTAGTTCCTCGGATGAGGGTTGTTAAAGGAACAGGAAAAGCCATCAACTACTTGACTCCTCCAAGAATCTTATTAGCTCTGGTCACTGCTTGGCTGCGACGAGGTCGGTGGTTTGACGAACAGGGGAAGGCTCTGTATGCAGCCGAGGCAGACAGAATCCGTAACTGGGCCGAAAACCGCACTCGTTTCTCCTTCACTGATGCAATGGAGATGTACACAGAAAACACCTGGGTGTCTGTCTTCTCCCTTTCAGTCGTTTGTGCCTTCATAATTCTCTCAACCACAAACACCACACTTCCCGGAACTTGA

Protein sequence

MSALTKLHVYPQSFDGPSTRDGHLIGALGRGVHRFGVGVSTSRGGLCLRKCRSFRGEDGGDFDKEDGKGRNRGKSRLKEVKVKKESQFWKFLRSGVLGKFNLLLGSDVERGKLVANMEGLFSSAAVQIGRYIVTMMSTGVILAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLEEHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPESVQETLRELEENLKGLIARSGSSNTSLAHAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQFTDEDITQILSTNLVGSLLCTREAMRVMRDQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKCGLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLVPRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEQGKALYAAEADRIRNWAENRTRFSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNTTLPGT
Homology
BLAST of Spg022161 vs. NCBI nr
Match: XP_038880096.1 (probable chlorophyll(ide) b reductase NYC1, chloroplastic [Benincasa hispida])

HSP 1 Score: 976.1 bits (2522), Expect = 1.2e-280
Identity = 493/522 (94.44%), Postives = 507/522 (97.13%), Query Frame = 0

Query: 1   MSALTKLHVYPQSFDG--PSTRDGHLIGALGRGVHRFGVGVSTSRGGLCLRKCRSFRGED 60
           M+ LTKLHVYPQSF+G  PSTRDGHLIGALGRG+HRFGVGVSTSR GLCLRKCRSFRG D
Sbjct: 1   MTTLTKLHVYPQSFEGPTPSTRDGHLIGALGRGIHRFGVGVSTSRSGLCLRKCRSFRGGD 60

Query: 61  GGDFDKEDGKGRNRGKSRLKEVKVKKESQFWKFLRSGVLGKFNLLLGSDVERGKLVANME 120
           GGDF+KED KGRNR  SRLKEVK+KKESQFWKFLRSGVLGKFNLLLGSDV++GKL+ANME
Sbjct: 61  GGDFEKEDSKGRNRRNSRLKEVKMKKESQFWKFLRSGVLGKFNLLLGSDVDQGKLMANME 120

Query: 121 GLFSSAAVQIGRYIVTMMSTGVILAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMV 180
           GL SSAAVQIGRYIVTMMSTGV+LAVGFQLSGGD+QMN LIWYSWLGGVIIGTMIGANMV
Sbjct: 121 GLLSSAAVQIGRYIVTMMSTGVVLAVGFQLSGGDNQMNALIWYSWLGGVIIGTMIGANMV 180

Query: 181 LEEHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPESVQETLRELEENLKGL 240
           LEEHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPESVQETLRELEENLKGL
Sbjct: 181 LEEHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPESVQETLRELEENLKGL 240

Query: 241 IARSGSSNTSLAHAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLL 300
           + RSGSSN SLA AKVVGTTCNVCDPEDVHNLANFA+NELGSIDIWINNAGTNKGFRPLL
Sbjct: 241 MVRSGSSNKSLACAKVVGTTCNVCDPEDVHNLANFAINELGSIDIWINNAGTNKGFRPLL 300

Query: 301 QFTDEDITQILSTNLVGSLLCTREAMRVMRDQAKGGHIFNMDGAGSGGSSTPLTAVYGST 360
           QFTDEDITQILSTNLVGSLLCTREAMRVMR+QAKGGHIFNMDGAGSGGSSTPLTAVYGST
Sbjct: 301 QFTDEDITQILSTNLVGSLLCTREAMRVMRNQAKGGHIFNMDGAGSGGSSTPLTAVYGST 360

Query: 361 KCGLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVART 420
           KCGLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVART
Sbjct: 361 KCGLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVART 420

Query: 421 LVPRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEQGKALYAAEADRIRNWAENR 480
           LVPRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEQG+ALYAAEADRIRNWAENR
Sbjct: 421 LVPRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEQGRALYAAEADRIRNWAENR 480

Query: 481 TRFSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNTTLPGT 521
           TRFSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTN T PGT
Sbjct: 481 TRFSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNNTFPGT 522

BLAST of Spg022161 vs. NCBI nr
Match: XP_023529434.1 (probable chlorophyll(ide) b reductase NYC1, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 970.3 bits (2507), Expect = 6.6e-279
Identity = 489/520 (94.04%), Postives = 504/520 (96.92%), Query Frame = 0

Query: 1   MSALTKLHVYPQSFDGPSTRDGHLIGALGRGVHRFGVGVSTSRGGLCLRKCRSFRGEDGG 60
           M+ LTKL+VYPQSFDGPSTRDGHLIGALGRGVHRFGVGVSTSR GL LRKCRSFRGEDGG
Sbjct: 1   MTTLTKLYVYPQSFDGPSTRDGHLIGALGRGVHRFGVGVSTSRSGLRLRKCRSFRGEDGG 60

Query: 61  DFDKEDGKGRNRGKSRLKEVKVKKESQFWKFLRSGVLGKFNLLLGSDVERGKLVANMEGL 120
           D++KED KGR+RG SRLK+V  KKE QFWKFLRSGVLGK NLLLGSD ERGKL+ANME L
Sbjct: 61  DYEKEDRKGRSRGDSRLKKVSTKKEIQFWKFLRSGVLGKVNLLLGSDFERGKLMANMEAL 120

Query: 121 FSSAAVQIGRYIVTMMSTGVILAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLE 180
           FSSAAVQIGRYIVTMMSTGV+LAVGFQLSGGDS MNTLIWYSWLGGVIIGTMIGANMVLE
Sbjct: 121 FSSAAVQIGRYIVTMMSTGVVLAVGFQLSGGDSHMNTLIWYSWLGGVIIGTMIGANMVLE 180

Query: 181 EHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPESVQETLRELEENLKGLIA 240
           EHCRAGPRNVVITGSTRGLGKALAREFLLSGD VVVASRSPESVQET+RELEENLKGL+ 
Sbjct: 181 EHCRAGPRNVVITGSTRGLGKALAREFLLSGDHVVVASRSPESVQETIRELEENLKGLMV 240

Query: 241 RSGSSNTSLAHAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQF 300
           RSGSSNTSL HAKVVGTTCNVCDPEDVHNLA FALNELGSIDIWINNAGTNKGFRPLLQF
Sbjct: 241 RSGSSNTSLTHAKVVGTTCNVCDPEDVHNLAKFALNELGSIDIWINNAGTNKGFRPLLQF 300

Query: 301 TDEDITQILSTNLVGSLLCTREAMRVMRDQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC 360
           TDEDITQILSTNLVGSLLCTREAMRVMR+QAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC
Sbjct: 301 TDEDITQILSTNLVGSLLCTREAMRVMRNQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC 360

Query: 361 GLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV 420
           GLRQLQSSLLKEC+RSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV
Sbjct: 361 GLRQLQSSLLKECKRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV 420

Query: 421 PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEQGKALYAAEADRIRNWAENRTR 480
           PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFD++GKALYAAEADRIRNWAENRTR
Sbjct: 421 PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDDKGKALYAAEADRIRNWAENRTR 480

Query: 481 FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNTTLPGT 521
           FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTT+TTLPGT
Sbjct: 481 FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTSTTLPGT 520

BLAST of Spg022161 vs. NCBI nr
Match: KAG6587961.1 (putative chlorophyll(ide) b reductase NYC1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 969.1 bits (2504), Expect = 1.5e-278
Identity = 488/520 (93.85%), Postives = 505/520 (97.12%), Query Frame = 0

Query: 1   MSALTKLHVYPQSFDGPSTRDGHLIGALGRGVHRFGVGVSTSRGGLCLRKCRSFRGEDGG 60
           M+ LTKL+V PQSFDGPSTRDGHLIGALGRGVHRFGVGVSTSR GL LRKCRSFRGEDGG
Sbjct: 1   MTTLTKLYVCPQSFDGPSTRDGHLIGALGRGVHRFGVGVSTSRSGLSLRKCRSFRGEDGG 60

Query: 61  DFDKEDGKGRNRGKSRLKEVKVKKESQFWKFLRSGVLGKFNLLLGSDVERGKLVANMEGL 120
           D++KED KGR+RG SRLK+V  KKE+QFWKFLRSGVLGK NLLLGSD ERGKL+ANME L
Sbjct: 61  DYEKEDRKGRSRGDSRLKKVSTKKETQFWKFLRSGVLGKVNLLLGSDFERGKLMANMEAL 120

Query: 121 FSSAAVQIGRYIVTMMSTGVILAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLE 180
           FSSAAVQIGRYIVTMMSTGV+LAVGFQLSGGDSQMNTLIWYSWLGGVIIG+MIGANMVLE
Sbjct: 121 FSSAAVQIGRYIVTMMSTGVVLAVGFQLSGGDSQMNTLIWYSWLGGVIIGSMIGANMVLE 180

Query: 181 EHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPESVQETLRELEENLKGLIA 240
           EHCRAGPRNVVITGSTRGLGKALAREFLLSGD VVVASRSPESVQET+RELEENLKGL+ 
Sbjct: 181 EHCRAGPRNVVITGSTRGLGKALAREFLLSGDHVVVASRSPESVQETIRELEENLKGLMV 240

Query: 241 RSGSSNTSLAHAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQF 300
           RSGSSNTSL HAKVVGTTCNVCDPEDVHNLA FALNELGSIDIWINNAGTNKGFRPLLQF
Sbjct: 241 RSGSSNTSLTHAKVVGTTCNVCDPEDVHNLAKFALNELGSIDIWINNAGTNKGFRPLLQF 300

Query: 301 TDEDITQILSTNLVGSLLCTREAMRVMRDQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC 360
           TDEDITQILSTNLVGSLLCTREAMRVMR+QAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC
Sbjct: 301 TDEDITQILSTNLVGSLLCTREAMRVMRNQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC 360

Query: 361 GLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV 420
           GLRQLQSSLLKEC+RSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV
Sbjct: 361 GLRQLQSSLLKECKRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV 420

Query: 421 PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEQGKALYAAEADRIRNWAENRTR 480
           PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFD++GKALYAAEADRIRNWAENRTR
Sbjct: 421 PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDDKGKALYAAEADRIRNWAENRTR 480

Query: 481 FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNTTLPGT 521
           FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTT+TTLPGT
Sbjct: 481 FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTSTTLPGT 520

BLAST of Spg022161 vs. NCBI nr
Match: XP_022933701.1 (probable chlorophyll(ide) b reductase NYC1, chloroplastic [Cucurbita moschata])

HSP 1 Score: 967.6 bits (2500), Expect = 4.3e-278
Identity = 488/520 (93.85%), Postives = 503/520 (96.73%), Query Frame = 0

Query: 1   MSALTKLHVYPQSFDGPSTRDGHLIGALGRGVHRFGVGVSTSRGGLCLRKCRSFRGEDGG 60
           M+ LTKL+V PQSFDGPSTRDGHLIGALGRGVHRFGVGVSTSR GL LRKCRSFRGEDGG
Sbjct: 1   MTTLTKLYVCPQSFDGPSTRDGHLIGALGRGVHRFGVGVSTSRSGLSLRKCRSFRGEDGG 60

Query: 61  DFDKEDGKGRNRGKSRLKEVKVKKESQFWKFLRSGVLGKFNLLLGSDVERGKLVANMEGL 120
           D++KED KGR+ G SRLK+V  KKE+QFWKFLRSGVLGK NLLLGSD ERGKL+ANME L
Sbjct: 61  DYEKEDRKGRSGGDSRLKKVSTKKETQFWKFLRSGVLGKVNLLLGSDFERGKLMANMEAL 120

Query: 121 FSSAAVQIGRYIVTMMSTGVILAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLE 180
           FSSAAVQIGRYIVTMMSTGV+LAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLE
Sbjct: 121 FSSAAVQIGRYIVTMMSTGVVLAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLE 180

Query: 181 EHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPESVQETLRELEENLKGLIA 240
           EHCRAGPRNVVITGSTRGLGKALAREFLLSGD VVVASRSPESVQET+RELEENLKGL+ 
Sbjct: 181 EHCRAGPRNVVITGSTRGLGKALAREFLLSGDHVVVASRSPESVQETIRELEENLKGLMV 240

Query: 241 RSGSSNTSLAHAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQF 300
           RSGSSNTSL HAKVVGTTCNVCDPEDVHNLA FALNELGSIDIWINNAGTNKGFRPLLQF
Sbjct: 241 RSGSSNTSLTHAKVVGTTCNVCDPEDVHNLAKFALNELGSIDIWINNAGTNKGFRPLLQF 300

Query: 301 TDEDITQILSTNLVGSLLCTREAMRVMRDQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC 360
           TDEDITQILSTNLVGSLLCTREAMRVMR QAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC
Sbjct: 301 TDEDITQILSTNLVGSLLCTREAMRVMRSQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC 360

Query: 361 GLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV 420
           GLRQLQSSLLKEC+RSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV
Sbjct: 361 GLRQLQSSLLKECKRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV 420

Query: 421 PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEQGKALYAAEADRIRNWAENRTR 480
           PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFD++GKALYAAEADRIRNWAENRTR
Sbjct: 421 PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDDKGKALYAAEADRIRNWAENRTR 480

Query: 481 FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNTTLPGT 521
           FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTT+TTLPGT
Sbjct: 481 FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTSTTLPGT 520

BLAST of Spg022161 vs. NCBI nr
Match: XP_022965406.1 (probable chlorophyll(ide) b reductase NYC1, chloroplastic [Cucurbita maxima])

HSP 1 Score: 966.8 bits (2498), Expect = 7.3e-278
Identity = 489/520 (94.04%), Postives = 501/520 (96.35%), Query Frame = 0

Query: 1   MSALTKLHVYPQSFDGPSTRDGHLIGALGRGVHRFGVGVSTSRGGLCLRKCRSFRGEDGG 60
           M+ LTKL+V PQSFDGPSTRDGHLIGALGRGVHRFGVGVSTSR GL LRKCRSFRGEDGG
Sbjct: 1   MTTLTKLYVCPQSFDGPSTRDGHLIGALGRGVHRFGVGVSTSRSGLSLRKCRSFRGEDGG 60

Query: 61  DFDKEDGKGRNRGKSRLKEVKVKKESQFWKFLRSGVLGKFNLLLGSDVERGKLVANMEGL 120
           D +KED KGR+RG SRLK+V  KKE+QFWKFLRSG LGK NLLLGSD ERGKL+ANME L
Sbjct: 61  DNEKEDRKGRSRGDSRLKKVSTKKEAQFWKFLRSGFLGKVNLLLGSDFERGKLMANMEAL 120

Query: 121 FSSAAVQIGRYIVTMMSTGVILAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLE 180
           FSSAAVQIGRYIVTMMSTGV+LAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLE
Sbjct: 121 FSSAAVQIGRYIVTMMSTGVVLAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLE 180

Query: 181 EHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPESVQETLRELEENLKGLIA 240
           EHCRAGPRNVVITGSTRGLGKALAREFLLSGD VVVASRSPESVQET+RELEENLKGL+ 
Sbjct: 181 EHCRAGPRNVVITGSTRGLGKALAREFLLSGDHVVVASRSPESVQETIRELEENLKGLMI 240

Query: 241 RSGSSNTSLAHAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQF 300
           RSGSSNTSL HAKVVGTTCNVCDPEDVHNLA FALNELGSIDIWINNAGTNKGFRPLLQF
Sbjct: 241 RSGSSNTSLTHAKVVGTTCNVCDPEDVHNLAKFALNELGSIDIWINNAGTNKGFRPLLQF 300

Query: 301 TDEDITQILSTNLVGSLLCTREAMRVMRDQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC 360
           TDEDITQILSTNLVGSLLCTREAMRVMR QAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC
Sbjct: 301 TDEDITQILSTNLVGSLLCTREAMRVMRSQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC 360

Query: 361 GLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV 420
           GLRQLQSSLLKEC+RSNVGVH ASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV
Sbjct: 361 GLRQLQSSLLKECKRSNVGVHAASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV 420

Query: 421 PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEQGKALYAAEADRIRNWAENRTR 480
           PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDE+GKALYAAEADRIRNWAENRTR
Sbjct: 421 PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEKGKALYAAEADRIRNWAENRTR 480

Query: 481 FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNTTLPGT 521
           FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNTTLPGT
Sbjct: 481 FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNTTLPGT 520

BLAST of Spg022161 vs. ExPASy Swiss-Prot
Match: Q5N800 (Probable chlorophyll(ide) b reductase NYC1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=NYC1 PE=1 SV=1)

HSP 1 Score: 672.5 bits (1734), Expect = 3.7e-192
Identity = 354/482 (73.44%), Postives = 397/482 (82.37%), Query Frame = 0

Query: 48  LRKCRSFRGE--DGGDFDKED------GKGRNRGKSRLKEVKVKKESQFWKFLRSGVLGK 107
           L +CR+F+ E  +GG+            + R R K  L   K+K   Q     RS     
Sbjct: 31  LLRCRAFKQEADNGGEEASSSPPPPTTAEARRRRKGPL--YKLKAAIQGLAGSRSAAAEA 90

Query: 108 FNLLLGSDVERGKLVANMEGLFSSAAVQIGRYIVTMMSTGVILAVGFQLSGGDSQMNTLI 167
           +    G + +R   V   E +F S A Q+GRY++TMMS+GV+L VGFQLSGGDSQMNTLI
Sbjct: 91  Y----GGEYQRA--VEKAEEIFFSVATQVGRYVITMMSSGVVLGVGFQLSGGDSQMNTLI 150

Query: 168 WYSWLGGVIIGTMIGANMVLEEHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASR 227
           WYSWLGGVIIGTMIGAN VLEEHC+AGPRNVVITGSTRGLGKALAREFLLSGDRVV+ASR
Sbjct: 151 WYSWLGGVIIGTMIGANSVLEEHCKAGPRNVVITGSTRGLGKALAREFLLSGDRVVIASR 210

Query: 228 SPESVQETLRELEENL-KGLIARSGSSNTSLAHAKVVGTTCNVCDPEDVHNLANFALNEL 287
           SPESV +T+ ELEEN+ +GL          L HAKVVGT+C+VC PEDV  L NFA +EL
Sbjct: 211 SPESVLQTINELEENIQEGLSVAKKKQREILLHAKVVGTSCDVCKPEDVKKLVNFAKDEL 270

Query: 288 GSIDIWINNAGTNKGFRPLLQFTDEDITQILSTNLVGSLLCTREAMRVMRDQAKGGHIFN 347
           GSIDIWINNAGTNKGFRPL+ F+DEDI+QI+STNLVGSLLCTREAM VM+ Q KGGH+FN
Sbjct: 271 GSIDIWINNAGTNKGFRPLVNFSDEDISQIVSTNLVGSLLCTREAMNVMQHQQKGGHVFN 330

Query: 348 MDGAGSGGSSTPLTAVYGSTKCGLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGST 407
           MDGAGSGGSSTPLTAVYGSTKCGLRQ Q+SLLKE RRS VGVHTASPGMVLTDLLLSGS+
Sbjct: 331 MDGAGSGGSSTPLTAVYGSTKCGLRQFQASLLKESRRSKVGVHTASPGMVLTDLLLSGSS 390

Query: 408 VRNKQMFNIICELPETVARTLVPRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDE 467
           +RNKQMFN+ICELPETVARTLVPRMRVVKG+GKAINYLTPPRILLALVTAW+RRGRWFDE
Sbjct: 391 LRNKQMFNLICELPETVARTLVPRMRVVKGSGKAINYLTPPRILLALVTAWVRRGRWFDE 450

Query: 468 QGKALYAAEADRIRNWAENRTRFSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNTTLP 521
           +G+A+YAAEADRIRNWAE+R RFSFTDAMEMYTENTWVSVFSLSVVCAFIILS++   LP
Sbjct: 451 EGRAVYAAEADRIRNWAESRARFSFTDAMEMYTENTWVSVFSLSVVCAFIILSSSGGPLP 504

BLAST of Spg022161 vs. ExPASy Swiss-Prot
Match: Q93ZA0 (Probable chlorophyll(ide) b reductase NYC1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NYC1 PE=1 SV=1)

HSP 1 Score: 656.8 bits (1693), Expect = 2.1e-187
Identity = 334/447 (74.72%), Postives = 384/447 (85.91%), Query Frame = 0

Query: 76  RLKEVKVKKESQFWKFLRSGVLGKFNLLLGSDVERGKLVANMEGLFSSAAVQIGRYIVTM 135
           R ++V+  K +  W  L+SG LG   L   S  E  + V N+E +FSS AVQI RYIVTM
Sbjct: 50  RKRKVEKFKGNGSWDSLKSGFLGFSKLGFLSKDEYNQKVENLEMVFSSVAVQIARYIVTM 109

Query: 136 MSTGVILAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLEEHCRAGPRNVVITGS 195
            STG IL +GFQLSGGDS MN+L+WYSWLGG+IIGTM GANMVLE+H RAGPRNVVITGS
Sbjct: 110 TSTGAILLIGFQLSGGDSSMNSLVWYSWLGGIIIGTMTGANMVLEDHYRAGPRNVVITGS 169

Query: 196 TRGLGKALAREFLLSGDRVVVASRSPESVQETLRELEENLKGLIAR-SGSSNTSLAHAKV 255
           TRGLGKALAREFLLSGDRV+V SRS ESV  T++ELE+NLK +++  S S+   L+ AKV
Sbjct: 170 TRGLGKALAREFLLSGDRVIVTSRSSESVDMTVKELEQNLKEIMSNASESARKKLSDAKV 229

Query: 256 VGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQFTDEDITQILSTNLV 315
           VG  C+VC PEDV  L+NFA+ ELGSI+IWINNAGTNKGFRPLL+FT+EDITQI+STNL+
Sbjct: 230 VGIACDVCKPEDVEKLSNFAVKELGSINIWINNAGTNKGFRPLLEFTEEDITQIVSTNLI 289

Query: 316 GSLLCTREAMRVMRDQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKCGLRQLQSSLLKECR 375
           GS+LCTR AM VM  Q  GGHIFNMDGAGSGGSSTPLTAVYGSTKCGLRQ   S++KE +
Sbjct: 290 GSILCTRGAMDVMSRQHSGGHIFNMDGAGSGGSSTPLTAVYGSTKCGLRQFHGSIVKESQ 349

Query: 376 RSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLVPRMRVVKGTGKAIN 435
           ++NVG+HTASPGMVLT+LLLSGS+++NKQMFNIICELPETVARTLVPRMRVVKG+GKA+N
Sbjct: 350 KTNVGLHTASPGMVLTELLLSGSSIKNKQMFNIICELPETVARTLVPRMRVVKGSGKAVN 409

Query: 436 YLTPPRILLALVTAWLRRGRWFDEQGKALYAAEADRIRNWAENRTRFSFTDAMEMYTENT 495
           YLTPPRILLA+VT+WLRRGRWFD+QG+ALYAAEADR+RNWAENRTR S TDAMEMYTENT
Sbjct: 410 YLTPPRILLAIVTSWLRRGRWFDDQGRALYAAEADRLRNWAENRTRLSLTDAMEMYTENT 469

Query: 496 WVSVFSLSVVCAFIIL-STTNTTLPGT 521
           WVSVFSLSVVCAFIIL STT ++ PGT
Sbjct: 470 WVSVFSLSVVCAFIILQSTTPSSFPGT 496

BLAST of Spg022161 vs. ExPASy Swiss-Prot
Match: Q8LEU3 (Chlorophyll(ide) b reductase NOL, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NOL PE=1 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 1.3e-51
Identity = 120/285 (42.11%), Postives = 168/285 (58.95%), Query Frame = 0

Query: 187 PRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPESVQETLRELEENLKGLIARSGSSN 246
           P N++ITGST+G+G ALAREFL +GD VV+ SRS E V+  ++ L+E             
Sbjct: 79  PYNILITGSTKGIGYALAREFLKAGDNVVICSRSAERVETAVQSLKEEF----------- 138

Query: 247 TSLAHAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNK-GFRPLLQFTDEDI 306
                  V GT C+V + +DV  L  ++   L  IDIWINNAG+N   F+PL + +DED+
Sbjct: 139 ----GEHVWGTKCDVTEGKDVRELVAYSQKNLKYIDIWINNAGSNAYSFKPLAEASDEDL 198

Query: 307 TQILSTNLVGSLLCTREAMRVMRDQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKCGLRQL 366
            +++ TN +G +LC REAM +M  Q++GGHIFN+DGAGS G  TP  A YG+TK  +  L
Sbjct: 199 IEVVKTNTLGLMLCCREAMNMMLTQSRGGHIFNIDGAGSDGRPTPRFAAYGATKRSVVHL 258

Query: 367 QSSLLKECRR---SNVGVHTASPGMVLTDLLLSGSTVRNKQMF-NIICELPETVARTLVP 426
             SL  E +     NV VH  SPGMV TDLL+SG+T +  + F N++ E  E VA  LVP
Sbjct: 259 TKSLQAELQMQDVKNVVVHNLSPGMVTTDLLMSGATTKQAKFFINVLAEPAEVVAEYLVP 318

Query: 427 RMRVVKGTGKA----INYLTPPRI---LLALVTAWLRRGRWFDEQ 460
            +R +  +G      I +LT  +    + + V    R+ R+  E+
Sbjct: 319 NIRAIPASGSMKPTYIRFLTGIKAYTKIFSRVALGARKNRYVTEE 348

BLAST of Spg022161 vs. ExPASy Swiss-Prot
Match: Q84ST4 (Chlorophyll(ide) b reductase NOL, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=NOL PE=1 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 2.7e-49
Identity = 111/266 (41.73%), Postives = 161/266 (60.53%), Query Frame = 0

Query: 166 GVIIGTMIGANMVLEEHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPESVQ 225
           G+    + G   V        P NV+ITGST+G+G ALA+EFL +GD VV+ SRS E V+
Sbjct: 52  GIRAEAVPGGGGVARRAAMVPPYNVLITGSTKGIGYALAKEFLKAGDNVVICSRSAERVE 111

Query: 226 ETLRELEENLKGLIARSGSSNTSLAHAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWI 285
             + +L++                    V G  C+V + +DV  L +FA +++  IDIWI
Sbjct: 112 SAVTDLKK--------------EFGEQHVWGIVCDVREGKDVKALVDFARDKMKYIDIWI 171

Query: 286 NNAGTNK-GFRPLLQFTDEDITQILSTNLVGSLLCTREAMRVMRDQAKGGHIFNMDGAGS 345
           NNAG+N   ++PL++ +DE + ++++TN +G ++C REA+ +MR+Q +GGHIFN+DGAGS
Sbjct: 172 NNAGSNAYSYKPLVETSDEALMEVITTNTLGLMICCREAINMMRNQPRGGHIFNIDGAGS 231

Query: 346 GGSSTPLTAVYGSTKCGLRQLQSSLLKECRR---SNVGVHTASPGMVLTDLLLSGSTVRN 405
            G  TP  A YG+TK  +  L  SL  E +    +NV VH  SPGMV TDLL+SG+T + 
Sbjct: 232 DGRPTPRFAAYGATKRSVVHLTKSLQAELQMNEVNNVMVHNLSPGMVTTDLLMSGATTKQ 291

Query: 406 KQMF-NIICELPETVARTLVPRMRVV 427
            + F NI+ E    VA  LVP +R +
Sbjct: 292 AKFFINILAEPANVVADYLVPNIRAI 303

BLAST of Spg022161 vs. ExPASy Swiss-Prot
Match: P39483 (Glucose 1-dehydrogenase 2 OS=Bacillus megaterium OX=1404 GN=gdhII PE=3 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 3.3e-15
Identity = 62/204 (30.39%), Postives = 98/204 (48.04%), Query Frame = 0

Query: 190 VVITGSTRGLGKALAREFLLSGDRVVVASRS-PESVQETLRELEENLKGLIARSGSSNTS 249
           VV+TG ++GLG+A+A  F     +VVV  RS  E   E  +E+EE               
Sbjct: 10  VVVTGGSKGLGRAMAVRFGQEQSKVVVNYRSNEEEALEVKKEIEE--------------- 69

Query: 250 LAHAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQFTDEDITQI 309
            A  + +    +V   EDV NL   A+ E GS+D+ INNAG      P  + + E+  Q+
Sbjct: 70  -AGGQAIIVRGDVTKEEDVVNLVETAVKEFGSLDVMINNAGVENPV-PSHELSLENWNQV 129

Query: 310 LSTNLVGSLLCTREAMRVMRDQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKCGLRQLQSS 369
           + TNL G+ L +REA++   +    G++ NM          PL   Y ++K G++ +  +
Sbjct: 130 IDTNLTGAFLGSREAIKYFVENDIKGNVINMSSVHE-MIPWPLFVHYAASKGGMKLMTET 189

Query: 370 LLKECRRSNVGVHTASPGMVLTDL 393
           L  E     + V+   PG + T +
Sbjct: 190 LALEYAPKGIRVNNIGPGAIDTPI 195

BLAST of Spg022161 vs. ExPASy TrEMBL
Match: A0A6J1EZT2 (probable chlorophyll(Ide) b reductase NYC1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111441038 PE=4 SV=1)

HSP 1 Score: 967.6 bits (2500), Expect = 2.1e-278
Identity = 488/520 (93.85%), Postives = 503/520 (96.73%), Query Frame = 0

Query: 1   MSALTKLHVYPQSFDGPSTRDGHLIGALGRGVHRFGVGVSTSRGGLCLRKCRSFRGEDGG 60
           M+ LTKL+V PQSFDGPSTRDGHLIGALGRGVHRFGVGVSTSR GL LRKCRSFRGEDGG
Sbjct: 1   MTTLTKLYVCPQSFDGPSTRDGHLIGALGRGVHRFGVGVSTSRSGLSLRKCRSFRGEDGG 60

Query: 61  DFDKEDGKGRNRGKSRLKEVKVKKESQFWKFLRSGVLGKFNLLLGSDVERGKLVANMEGL 120
           D++KED KGR+ G SRLK+V  KKE+QFWKFLRSGVLGK NLLLGSD ERGKL+ANME L
Sbjct: 61  DYEKEDRKGRSGGDSRLKKVSTKKETQFWKFLRSGVLGKVNLLLGSDFERGKLMANMEAL 120

Query: 121 FSSAAVQIGRYIVTMMSTGVILAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLE 180
           FSSAAVQIGRYIVTMMSTGV+LAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLE
Sbjct: 121 FSSAAVQIGRYIVTMMSTGVVLAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLE 180

Query: 181 EHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPESVQETLRELEENLKGLIA 240
           EHCRAGPRNVVITGSTRGLGKALAREFLLSGD VVVASRSPESVQET+RELEENLKGL+ 
Sbjct: 181 EHCRAGPRNVVITGSTRGLGKALAREFLLSGDHVVVASRSPESVQETIRELEENLKGLMV 240

Query: 241 RSGSSNTSLAHAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQF 300
           RSGSSNTSL HAKVVGTTCNVCDPEDVHNLA FALNELGSIDIWINNAGTNKGFRPLLQF
Sbjct: 241 RSGSSNTSLTHAKVVGTTCNVCDPEDVHNLAKFALNELGSIDIWINNAGTNKGFRPLLQF 300

Query: 301 TDEDITQILSTNLVGSLLCTREAMRVMRDQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC 360
           TDEDITQILSTNLVGSLLCTREAMRVMR QAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC
Sbjct: 301 TDEDITQILSTNLVGSLLCTREAMRVMRSQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC 360

Query: 361 GLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV 420
           GLRQLQSSLLKEC+RSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV
Sbjct: 361 GLRQLQSSLLKECKRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV 420

Query: 421 PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEQGKALYAAEADRIRNWAENRTR 480
           PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFD++GKALYAAEADRIRNWAENRTR
Sbjct: 421 PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDDKGKALYAAEADRIRNWAENRTR 480

Query: 481 FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNTTLPGT 521
           FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTT+TTLPGT
Sbjct: 481 FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTSTTLPGT 520

BLAST of Spg022161 vs. ExPASy TrEMBL
Match: A0A6J1HLL1 (probable chlorophyll(Ide) b reductase NYC1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111465318 PE=4 SV=1)

HSP 1 Score: 966.8 bits (2498), Expect = 3.5e-278
Identity = 489/520 (94.04%), Postives = 501/520 (96.35%), Query Frame = 0

Query: 1   MSALTKLHVYPQSFDGPSTRDGHLIGALGRGVHRFGVGVSTSRGGLCLRKCRSFRGEDGG 60
           M+ LTKL+V PQSFDGPSTRDGHLIGALGRGVHRFGVGVSTSR GL LRKCRSFRGEDGG
Sbjct: 1   MTTLTKLYVCPQSFDGPSTRDGHLIGALGRGVHRFGVGVSTSRSGLSLRKCRSFRGEDGG 60

Query: 61  DFDKEDGKGRNRGKSRLKEVKVKKESQFWKFLRSGVLGKFNLLLGSDVERGKLVANMEGL 120
           D +KED KGR+RG SRLK+V  KKE+QFWKFLRSG LGK NLLLGSD ERGKL+ANME L
Sbjct: 61  DNEKEDRKGRSRGDSRLKKVSTKKEAQFWKFLRSGFLGKVNLLLGSDFERGKLMANMEAL 120

Query: 121 FSSAAVQIGRYIVTMMSTGVILAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLE 180
           FSSAAVQIGRYIVTMMSTGV+LAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLE
Sbjct: 121 FSSAAVQIGRYIVTMMSTGVVLAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLE 180

Query: 181 EHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPESVQETLRELEENLKGLIA 240
           EHCRAGPRNVVITGSTRGLGKALAREFLLSGD VVVASRSPESVQET+RELEENLKGL+ 
Sbjct: 181 EHCRAGPRNVVITGSTRGLGKALAREFLLSGDHVVVASRSPESVQETIRELEENLKGLMI 240

Query: 241 RSGSSNTSLAHAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQF 300
           RSGSSNTSL HAKVVGTTCNVCDPEDVHNLA FALNELGSIDIWINNAGTNKGFRPLLQF
Sbjct: 241 RSGSSNTSLTHAKVVGTTCNVCDPEDVHNLAKFALNELGSIDIWINNAGTNKGFRPLLQF 300

Query: 301 TDEDITQILSTNLVGSLLCTREAMRVMRDQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC 360
           TDEDITQILSTNLVGSLLCTREAMRVMR QAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC
Sbjct: 301 TDEDITQILSTNLVGSLLCTREAMRVMRSQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKC 360

Query: 361 GLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV 420
           GLRQLQSSLLKEC+RSNVGVH ASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV
Sbjct: 361 GLRQLQSSLLKECKRSNVGVHAASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLV 420

Query: 421 PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEQGKALYAAEADRIRNWAENRTR 480
           PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDE+GKALYAAEADRIRNWAENRTR
Sbjct: 421 PRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEKGKALYAAEADRIRNWAENRTR 480

Query: 481 FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNTTLPGT 521
           FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNTTLPGT
Sbjct: 481 FSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNTTLPGT 520

BLAST of Spg022161 vs. ExPASy TrEMBL
Match: A0A1S3CJ66 (probable chlorophyll(Ide) b reductase NYC1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501561 PE=4 SV=1)

HSP 1 Score: 964.1 bits (2491), Expect = 2.3e-277
Identity = 488/521 (93.67%), Postives = 503/521 (96.55%), Query Frame = 0

Query: 1   MSALTKLHVYPQSFDGPSTRDGHLIGALGRGVHRFGVGVSTSRG-GLCLRKCRSFRGEDG 60
           M+ L KLHVYPQ+F+GPSTRDGHLIGALG GVHR  VGVSTSRG GLCLRKCRSFRG DG
Sbjct: 1   MTTLAKLHVYPQTFEGPSTRDGHLIGALGPGVHRLDVGVSTSRGRGLCLRKCRSFRGGDG 60

Query: 61  GDFDKEDGKGRNRGKSRLKEVKVKKESQFWKFLRSGVLGKFNLLLGSDVERGKLVANMEG 120
           GDF+KE+ KG NR  SRLKEVK+KKESQFWKFLRSGVLGKFNLL+GSDV++GKL+ANMEG
Sbjct: 61  GDFEKENSKGGNRRNSRLKEVKMKKESQFWKFLRSGVLGKFNLLMGSDVDQGKLMANMEG 120

Query: 121 LFSSAAVQIGRYIVTMMSTGVILAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVL 180
           L SSAAVQIGRYIVTMMSTGV+LAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVL
Sbjct: 121 LLSSAAVQIGRYIVTMMSTGVVLAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVL 180

Query: 181 EEHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPESVQETLRELEENLKGLI 240
           EEHC AGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRS ESVQETLRELEENLKGL+
Sbjct: 181 EEHCSAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSSESVQETLRELEENLKGLM 240

Query: 241 ARSGSSNTSLAHAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQ 300
            RSGSSN SLAHAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQ
Sbjct: 241 VRSGSSNKSLAHAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQ 300

Query: 301 FTDEDITQILSTNLVGSLLCTREAMRVMRDQAKGGHIFNMDGAGSGGSSTPLTAVYGSTK 360
           FTDEDITQILSTNLVGSLLCTREAMRVMR+Q KGGHIFNMDGAGSGGSSTPLTAVYGSTK
Sbjct: 301 FTDEDITQILSTNLVGSLLCTREAMRVMRNQPKGGHIFNMDGAGSGGSSTPLTAVYGSTK 360

Query: 361 CGLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTL 420
           CGLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTL
Sbjct: 361 CGLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTL 420

Query: 421 VPRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEQGKALYAAEADRIRNWAENRT 480
           VPRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFD+QG+ALYAAEADRIRNWAENRT
Sbjct: 421 VPRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDDQGRALYAAEADRIRNWAENRT 480

Query: 481 RFSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNTTLPGT 521
           RFSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTN TLPGT
Sbjct: 481 RFSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNNTLPGT 521

BLAST of Spg022161 vs. ExPASy TrEMBL
Match: A0A5D3DZ70 (Putative chlorophyll(Ide) b reductase NYC1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold403G00120 PE=4 SV=1)

HSP 1 Score: 962.6 bits (2487), Expect = 6.6e-277
Identity = 487/521 (93.47%), Postives = 502/521 (96.35%), Query Frame = 0

Query: 1   MSALTKLHVYPQSFDGPSTRDGHLIGALGRGVHRFGVGVSTSRG-GLCLRKCRSFRGEDG 60
           M+ L KLHVYPQ+F+GPSTRDGHLIGA G GVHR  VGVSTSRG GLCLRKCRSFRG DG
Sbjct: 1   MTTLAKLHVYPQTFEGPSTRDGHLIGARGPGVHRLDVGVSTSRGRGLCLRKCRSFRGGDG 60

Query: 61  GDFDKEDGKGRNRGKSRLKEVKVKKESQFWKFLRSGVLGKFNLLLGSDVERGKLVANMEG 120
           GDF+KE+ KG NR  SRLKEVK+KKESQFWKFLRSGVLGKFNLL+GSDV++GKL+ANMEG
Sbjct: 61  GDFEKENSKGGNRRNSRLKEVKIKKESQFWKFLRSGVLGKFNLLMGSDVDQGKLMANMEG 120

Query: 121 LFSSAAVQIGRYIVTMMSTGVILAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVL 180
           L SSAAVQIGRYIVTMMSTGV+LAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVL
Sbjct: 121 LLSSAAVQIGRYIVTMMSTGVVLAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVL 180

Query: 181 EEHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPESVQETLRELEENLKGLI 240
           EEHC AGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRS ESVQETLRELEENLKGL+
Sbjct: 181 EEHCSAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSSESVQETLRELEENLKGLM 240

Query: 241 ARSGSSNTSLAHAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQ 300
            RSGSSN SLAHAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQ
Sbjct: 241 VRSGSSNKSLAHAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQ 300

Query: 301 FTDEDITQILSTNLVGSLLCTREAMRVMRDQAKGGHIFNMDGAGSGGSSTPLTAVYGSTK 360
           FTDEDITQILSTNLVGSLLCTREAMRVMR+Q KGGHIFNMDGAGSGGSSTPLTAVYGSTK
Sbjct: 301 FTDEDITQILSTNLVGSLLCTREAMRVMRNQPKGGHIFNMDGAGSGGSSTPLTAVYGSTK 360

Query: 361 CGLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTL 420
           CGLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTL
Sbjct: 361 CGLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTL 420

Query: 421 VPRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEQGKALYAAEADRIRNWAENRT 480
           VPRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFD+QG+ALYAAEADRIRNWAENRT
Sbjct: 421 VPRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDDQGRALYAAEADRIRNWAENRT 480

Query: 481 RFSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNTTLPGT 521
           RFSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTN TLPGT
Sbjct: 481 RFSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNNTLPGT 521

BLAST of Spg022161 vs. ExPASy TrEMBL
Match: A0A0A0LTA8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G250130 PE=4 SV=1)

HSP 1 Score: 957.2 bits (2473), Expect = 2.8e-275
Identity = 484/521 (92.90%), Postives = 499/521 (95.78%), Query Frame = 0

Query: 1   MSALTKLHVYPQSFDGPSTRDGHLIGALGRGVHRFGVGVSTSRG-GLCLRKCRSFRGEDG 60
           M+ L KLHVYPQ+FDGPSTRDGHL GALG G+HRF  GVST RG GLCLRKCRSFRG DG
Sbjct: 1   MTTLAKLHVYPQTFDGPSTRDGHLFGALGPGLHRFDAGVSTRRGRGLCLRKCRSFRGGDG 60

Query: 61  GDFDKEDGKGRNRGKSRLKEVKVKKESQFWKFLRSGVLGKFNLLLGSDVERGKLVANMEG 120
           GDF+KE+ KGRNR  SRLKEVK+KKESQFWK LRSGVLGKFNLL+GSDV++GKL+ANMEG
Sbjct: 61  GDFEKENSKGRNRRNSRLKEVKMKKESQFWKLLRSGVLGKFNLLMGSDVDQGKLMANMEG 120

Query: 121 LFSSAAVQIGRYIVTMMSTGVILAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVL 180
           L SSAAVQIGRYIVTMMSTGV+LAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVL
Sbjct: 121 LLSSAAVQIGRYIVTMMSTGVVLAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVL 180

Query: 181 EEHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPESVQETLRELEENLKGLI 240
           EEHC AGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRS ESVQETLRELEENLKGL+
Sbjct: 181 EEHCSAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSSESVQETLRELEENLKGLM 240

Query: 241 ARSGSSNTSLAHAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQ 300
            RSGSSN SLAHAKVVGTTCNVCDPEDVH LANF LNELGSIDIWINNAGTNKGFRPLLQ
Sbjct: 241 VRSGSSNKSLAHAKVVGTTCNVCDPEDVHKLANFTLNELGSIDIWINNAGTNKGFRPLLQ 300

Query: 301 FTDEDITQILSTNLVGSLLCTREAMRVMRDQAKGGHIFNMDGAGSGGSSTPLTAVYGSTK 360
           FTDEDITQILSTNLVGSLLCTREAMRVMR+QAKGG IFNMDGAGSGGSSTPLTAVYGSTK
Sbjct: 301 FTDEDITQILSTNLVGSLLCTREAMRVMRNQAKGGQIFNMDGAGSGGSSTPLTAVYGSTK 360

Query: 361 CGLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTL 420
           CGLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTL
Sbjct: 361 CGLRQLQSSLLKECRRSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTL 420

Query: 421 VPRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDEQGKALYAAEADRIRNWAENRT 480
           VPRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFD+QG+ALYAAEADRIRNWAENRT
Sbjct: 421 VPRMRVVKGTGKAINYLTPPRILLALVTAWLRRGRWFDDQGRALYAAEADRIRNWAENRT 480

Query: 481 RFSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNTTLPGT 521
           RFSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTN TLPGT
Sbjct: 481 RFSFTDAMEMYTENTWVSVFSLSVVCAFIILSTTNNTLPGT 521

BLAST of Spg022161 vs. TAIR 10
Match: AT4G13250.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 656.8 bits (1693), Expect = 1.5e-188
Identity = 334/447 (74.72%), Postives = 384/447 (85.91%), Query Frame = 0

Query: 76  RLKEVKVKKESQFWKFLRSGVLGKFNLLLGSDVERGKLVANMEGLFSSAAVQIGRYIVTM 135
           R ++V+  K +  W  L+SG LG   L   S  E  + V N+E +FSS AVQI RYIVTM
Sbjct: 50  RKRKVEKFKGNGSWDSLKSGFLGFSKLGFLSKDEYNQKVENLEMVFSSVAVQIARYIVTM 109

Query: 136 MSTGVILAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLEEHCRAGPRNVVITGS 195
            STG IL +GFQLSGGDS MN+L+WYSWLGG+IIGTM GANMVLE+H RAGPRNVVITGS
Sbjct: 110 TSTGAILLIGFQLSGGDSSMNSLVWYSWLGGIIIGTMTGANMVLEDHYRAGPRNVVITGS 169

Query: 196 TRGLGKALAREFLLSGDRVVVASRSPESVQETLRELEENLKGLIAR-SGSSNTSLAHAKV 255
           TRGLGKALAREFLLSGDRV+V SRS ESV  T++ELE+NLK +++  S S+   L+ AKV
Sbjct: 170 TRGLGKALAREFLLSGDRVIVTSRSSESVDMTVKELEQNLKEIMSNASESARKKLSDAKV 229

Query: 256 VGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQFTDEDITQILSTNLV 315
           VG  C+VC PEDV  L+NFA+ ELGSI+IWINNAGTNKGFRPLL+FT+EDITQI+STNL+
Sbjct: 230 VGIACDVCKPEDVEKLSNFAVKELGSINIWINNAGTNKGFRPLLEFTEEDITQIVSTNLI 289

Query: 316 GSLLCTREAMRVMRDQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKCGLRQLQSSLLKECR 375
           GS+LCTR AM VM  Q  GGHIFNMDGAGSGGSSTPLTAVYGSTKCGLRQ   S++KE +
Sbjct: 290 GSILCTRGAMDVMSRQHSGGHIFNMDGAGSGGSSTPLTAVYGSTKCGLRQFHGSIVKESQ 349

Query: 376 RSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLVPRMRVVKGTGKAIN 435
           ++NVG+HTASPGMVLT+LLLSGS+++NKQMFNIICELPETVARTLVPRMRVVKG+GKA+N
Sbjct: 350 KTNVGLHTASPGMVLTELLLSGSSIKNKQMFNIICELPETVARTLVPRMRVVKGSGKAVN 409

Query: 436 YLTPPRILLALVTAWLRRGRWFDEQGKALYAAEADRIRNWAENRTRFSFTDAMEMYTENT 495
           YLTPPRILLA+VT+WLRRGRWFD+QG+ALYAAEADR+RNWAENRTR S TDAMEMYTENT
Sbjct: 410 YLTPPRILLAIVTSWLRRGRWFDDQGRALYAAEADRLRNWAENRTRLSLTDAMEMYTENT 469

Query: 496 WVSVFSLSVVCAFIIL-STTNTTLPGT 521
           WVSVFSLSVVCAFIIL STT ++ PGT
Sbjct: 470 WVSVFSLSVVCAFIILQSTTPSSFPGT 496

BLAST of Spg022161 vs. TAIR 10
Match: AT4G13250.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 648.3 bits (1671), Expect = 5.3e-186
Identity = 332/447 (74.27%), Postives = 382/447 (85.46%), Query Frame = 0

Query: 76  RLKEVKVKKESQFWKFLRSGVLGKFNLLLGSDVERGKLVANMEGLFSSAAVQIGRYIVTM 135
           R ++V+  K +  W  L+SG LG   L   S  E  + V N+E +FSS AVQI RYIVTM
Sbjct: 50  RKRKVEKFKGNGSWDSLKSGFLGFSKLGFLSKDEYNQKVENLEMVFSSVAVQIARYIVTM 109

Query: 136 MSTGVILAVGFQLSGGDSQMNTLIWYSWLGGVIIGTMIGANMVLEEHCRAGPRNVVITGS 195
            STG IL +GFQLSGGDS MN+L+WYSWLGG+IIGTM GANMVLE+H RAGPRNVVITG 
Sbjct: 110 TSTGAILLIGFQLSGGDSSMNSLVWYSWLGGIIIGTMTGANMVLEDHYRAGPRNVVITG- 169

Query: 196 TRGLGKALAREFLLSGDRVVVASRSPESVQETLRELEENLKGLIAR-SGSSNTSLAHAKV 255
            RGLGKALAREFLLSGDRV+V SRS ESV  T++ELE+NLK +++  S S+   L+ AKV
Sbjct: 170 -RGLGKALAREFLLSGDRVIVTSRSSESVDMTVKELEQNLKEIMSNASESARKKLSDAKV 229

Query: 256 VGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQFTDEDITQILSTNLV 315
           VG  C+VC PEDV  L+NFA+ ELGSI+IWINNAGTNKGFRPLL+FT+EDITQI+STNL+
Sbjct: 230 VGIACDVCKPEDVEKLSNFAVKELGSINIWINNAGTNKGFRPLLEFTEEDITQIVSTNLI 289

Query: 316 GSLLCTREAMRVMRDQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKCGLRQLQSSLLKECR 375
           GS+LCTR AM VM  Q  GGHIFNMDGAGSGGSSTPLTAVYGSTKCGLRQ   S++KE +
Sbjct: 290 GSILCTRGAMDVMSRQHSGGHIFNMDGAGSGGSSTPLTAVYGSTKCGLRQFHGSIVKESQ 349

Query: 376 RSNVGVHTASPGMVLTDLLLSGSTVRNKQMFNIICELPETVARTLVPRMRVVKGTGKAIN 435
           ++NVG+HTASPGMVLT+LLLSGS+++NKQMFNIICELPETVARTLVPRMRVVKG+GKA+N
Sbjct: 350 KTNVGLHTASPGMVLTELLLSGSSIKNKQMFNIICELPETVARTLVPRMRVVKGSGKAVN 409

Query: 436 YLTPPRILLALVTAWLRRGRWFDEQGKALYAAEADRIRNWAENRTRFSFTDAMEMYTENT 495
           YLTPPRILLA+VT+WLRRGRWFD+QG+ALYAAEADR+RNWAENRTR S TDAMEMYTENT
Sbjct: 410 YLTPPRILLAIVTSWLRRGRWFDDQGRALYAAEADRLRNWAENRTRLSLTDAMEMYTENT 469

Query: 496 WVSVFSLSVVCAFIIL-STTNTTLPGT 521
           WVSVFSLSVVCAFIIL STT ++ PGT
Sbjct: 470 WVSVFSLSVVCAFIILQSTTPSSFPGT 494

BLAST of Spg022161 vs. TAIR 10
Match: AT5G04900.1 (NYC1-like )

HSP 1 Score: 205.7 bits (522), Expect = 9.1e-53
Identity = 120/285 (42.11%), Postives = 168/285 (58.95%), Query Frame = 0

Query: 187 PRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPESVQETLRELEENLKGLIARSGSSN 246
           P N++ITGST+G+G ALAREFL +GD VV+ SRS E V+  ++ L+E             
Sbjct: 79  PYNILITGSTKGIGYALAREFLKAGDNVVICSRSAERVETAVQSLKEEF----------- 138

Query: 247 TSLAHAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNK-GFRPLLQFTDEDI 306
                  V GT C+V + +DV  L  ++   L  IDIWINNAG+N   F+PL + +DED+
Sbjct: 139 ----GEHVWGTKCDVTEGKDVRELVAYSQKNLKYIDIWINNAGSNAYSFKPLAEASDEDL 198

Query: 307 TQILSTNLVGSLLCTREAMRVMRDQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKCGLRQL 366
            +++ TN +G +LC REAM +M  Q++GGHIFN+DGAGS G  TP  A YG+TK  +  L
Sbjct: 199 IEVVKTNTLGLMLCCREAMNMMLTQSRGGHIFNIDGAGSDGRPTPRFAAYGATKRSVVHL 258

Query: 367 QSSLLKECRR---SNVGVHTASPGMVLTDLLLSGSTVRNKQMF-NIICELPETVARTLVP 426
             SL  E +     NV VH  SPGMV TDLL+SG+T +  + F N++ E  E VA  LVP
Sbjct: 259 TKSLQAELQMQDVKNVVVHNLSPGMVTTDLLMSGATTKQAKFFINVLAEPAEVVAEYLVP 318

Query: 427 RMRVVKGTGKA----INYLTPPRI---LLALVTAWLRRGRWFDEQ 460
            +R +  +G      I +LT  +    + + V    R+ R+  E+
Sbjct: 319 NIRAIPASGSMKPTYIRFLTGIKAYTKIFSRVALGARKNRYVTEE 348

BLAST of Spg022161 vs. TAIR 10
Match: AT1G24360.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 76.3 bits (186), Expect = 8.4e-14
Identity = 57/204 (27.94%), Postives = 99/204 (48.53%), Query Frame = 0

Query: 190 VVITGSTRGLGKALAREFLLSGDRVVV-ASRSPESVQETLRELEENLKGLIARSGSSNTS 249
           VVITG++RG+GKA+A     +G +V+V  +RS +  +E  +++EE     I   G     
Sbjct: 79  VVITGASRGIGKAIALALGKAGCKVLVNYARSAKEAEEVAKQIEEYGGQAITFGG----- 138

Query: 250 LAHAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPLLQFTDEDITQI 309
                      +V    DV  +   AL++ G+ID+ +NNAG  +    L++       ++
Sbjct: 139 -----------DVSKATDVDAMMKTALDKWGTIDVVVNNAGITRD-TLLIRMKQSQWDEV 198

Query: 310 LSTNLVGSLLCTREAMRVMRDQAKGGHIFNMDGAGSGGSSTPLTAVYGSTKCGLRQLQSS 369
           ++ NL G  LCT+ A+++M  + K G I N+     G       A Y + K G+     +
Sbjct: 199 IALNLTGVFLCTQAAVKIMM-KKKRGRIINISSV-VGLIGNIGQANYAAAKGGVISFSKT 258

Query: 370 LLKECRRSNVGVHTASPGMVLTDL 393
             +E    N+ V+   PG + +D+
Sbjct: 259 AAREGASRNINVNVVCPGFIASDM 263

BLAST of Spg022161 vs. TAIR 10
Match: AT3G55290.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 70.9 bits (172), Expect = 3.5e-12
Identity = 55/250 (22.00%), Postives = 106/250 (42.40%), Query Frame = 0

Query: 178 VLEEHCRAGPRNVVITGSTRGLGKALAREFLLSGDRVVVASRSPESVQETLRELEENLKG 237
           +LE  C    + V++TG++ G+G+ +  +   +G +V+ A+R  + +     E+      
Sbjct: 11  LLEPWCELKDKVVLVTGASSGIGREICLDLAKAGCQVIAAARRVDRLNSLCSEI------ 70

Query: 238 LIARSGSSNTSLAHAKVVGTTCNVCDPEDVHNLANFALNELGSIDIWINNAGTNKGFRPL 297
               S SS    A A  +  +    D   +      A +  G ID  INNAG     +  
Sbjct: 71  ---NSFSSTGIQAAALELDVS---SDAATIQKAVREAWDIFGKIDALINNAGIRGNVKSS 130

Query: 298 LQFTDEDITQILSTNLVGSLLCTREAMRVMRDQAKGGHIFNMDGAGSGGSSTPLTAVYGS 357
           L  ++++   +  TNL G  L ++    +MRD  +GG + N+          P    Y  
Sbjct: 131 LDLSEDEWDNVFKTNLKGPWLVSKHVCMLMRDAKRGGSVINISSIAGIRGMLPGGLAYAC 190

Query: 358 TKCGLRQLQSSLLKECRRSNVGVHTASPGMV---LTDLLLSGSTVRNKQMFNIICELPET 417
           +K G+  +   +  E     + V++ +PG+    +T  L+    ++N     +  ++ +T
Sbjct: 191 SKGGVDTMSRMMALELGVHKIRVNSIAPGLFKSEITQGLMQKEWLKNVTERTVPLKVQQT 248

Query: 418 VARTLVPRMR 425
           V   L   +R
Sbjct: 251 VDPGLTSLVR 248

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038880096.11.2e-28094.44probable chlorophyll(ide) b reductase NYC1, chloroplastic [Benincasa hispida][more]
XP_023529434.16.6e-27994.04probable chlorophyll(ide) b reductase NYC1, chloroplastic [Cucurbita pepo subsp.... [more]
KAG6587961.11.5e-27893.85putative chlorophyll(ide) b reductase NYC1, chloroplastic, partial [Cucurbita ar... [more]
XP_022933701.14.3e-27893.85probable chlorophyll(ide) b reductase NYC1, chloroplastic [Cucurbita moschata][more]
XP_022965406.17.3e-27894.04probable chlorophyll(ide) b reductase NYC1, chloroplastic [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q5N8003.7e-19273.44Probable chlorophyll(ide) b reductase NYC1, chloroplastic OS=Oryza sativa subsp.... [more]
Q93ZA02.1e-18774.72Probable chlorophyll(ide) b reductase NYC1, chloroplastic OS=Arabidopsis thalian... [more]
Q8LEU31.3e-5142.11Chlorophyll(ide) b reductase NOL, chloroplastic OS=Arabidopsis thaliana OX=3702 ... [more]
Q84ST42.7e-4941.73Chlorophyll(ide) b reductase NOL, chloroplastic OS=Oryza sativa subsp. japonica ... [more]
P394833.3e-1530.39Glucose 1-dehydrogenase 2 OS=Bacillus megaterium OX=1404 GN=gdhII PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1EZT22.1e-27893.85probable chlorophyll(Ide) b reductase NYC1, chloroplastic OS=Cucurbita moschata ... [more]
A0A6J1HLL13.5e-27894.04probable chlorophyll(Ide) b reductase NYC1, chloroplastic OS=Cucurbita maxima OX... [more]
A0A1S3CJ662.3e-27793.67probable chlorophyll(Ide) b reductase NYC1, chloroplastic OS=Cucumis melo OX=365... [more]
A0A5D3DZ706.6e-27793.47Putative chlorophyll(Ide) b reductase NYC1 OS=Cucumis melo var. makuwa OX=119469... [more]
A0A0A0LTA82.8e-27592.90Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G250130 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G13250.11.5e-18874.72NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G13250.25.3e-18674.27NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G04900.19.1e-5342.11NYC1-like [more]
AT1G24360.18.4e-1427.94NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G55290.13.5e-1222.00NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 355..374
score: 26.6
coord: 376..393
score: 28.91
coord: 189..206
score: 40.75
coord: 279..290
score: 52.58
IPR002347Short-chain dehydrogenase/reductase SDRPFAMPF00106adh_shortcoord: 189..392
e-value: 1.5E-34
score: 119.2
NoneNo IPR availableGENE3D3.40.50.720coord: 186..430
e-value: 5.3E-48
score: 165.7
NoneNo IPR availablePANTHERPTHR24314NON-SPECIFIC LIPID TRANSFER PROTEIN-RELATEDcoord: 95..517
NoneNo IPR availablePANTHERPTHR24314:SF21CHLOROPHYLL(IDE) B REDUCTASE NYC1, CHLOROPLASTIC-RELATEDcoord: 95..517
NoneNo IPR availableCDDcd05233SDR_ccoord: 190..420
e-value: 1.50388E-51
score: 173.624
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 188..418

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg022161.1Spg022161.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015996 chlorophyll catabolic process
biological_process GO:0010304 PSII associated light-harvesting complex II catabolic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009536 plastid
molecular_function GO:0034256 chlorophyll(ide) b reductase activity
molecular_function GO:0016491 oxidoreductase activity