Spg022160 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg022160 vs. NCBI nr
Match: XP_022987170.1 (alpha-dioxygenase 2 [Cucurbita maxima] >XP_022987171.1 alpha-dioxygenase 2 [Cucurbita maxima])

HSP 1 Score: 1268.4 bits (3281), Expect = 0.0e+00
Identity = 607/646 (93.96%), Postives = 621/646 (96.13%), Query Frame = 0

Query: 1   MGSSLFSSPFVHPQLQQIVAKMTLLDTLLFYVVHFVDKLGLWHRMPVFMGLAYLGIRRHL 60
           MG+SLFSSPFVHPQLQQIVAKMTLLDTLLFYVVHFVDKLGLWHRMPVFMGLAYLG+RRHL
Sbjct: 1   MGTSLFSSPFVHPQLQQIVAKMTLLDTLLFYVVHFVDKLGLWHRMPVFMGLAYLGLRRHL 60

Query: 61  HQRYNLLHVGAMYGQKYDHQQVCYRTADGSCNHPFDSLVGSQGTFFGRNMPPSTSPYGVL 120
           HQRYNLLHVG++YGQKYDHQQ CYRTADGSCNHP DS+VGSQGTFFGRNMPPSTSPYGVL
Sbjct: 61  HQRYNLLHVGSLYGQKYDHQQFCYRTADGSCNHPSDSVVGSQGTFFGRNMPPSTSPYGVL 120

Query: 121 DPHPTVVATKLLERKNYIDNGKQFNMIACSWIQFMIHDWIDHLEDTKQVELRAPDEVANG 180
           DPHPT+VA+KLLERKNYIDNGKQFNMIACSWIQFMIHDWIDHLEDTKQVELRAPDEVANG
Sbjct: 121 DPHPTMVASKLLERKNYIDNGKQFNMIACSWIQFMIHDWIDHLEDTKQVELRAPDEVANG 180

Query: 181 CPLKSFKFFRTKVVSTDSPYLKTGTLNTRTPWWDGSVIYGNNEEGMRRVRAFEDGKMKIA 240
           CPLKSFKFFRTKVVST SPYLKTGTLNTRTPWWDGSVIYGNNEEGMRRVRAF+DGKMKIA
Sbjct: 181 CPLKSFKFFRTKVVSTGSPYLKTGTLNTRTPWWDGSVIYGNNEEGMRRVRAFQDGKMKIA 240

Query: 241 GDGLLEHDEKGIPISGDVRNCWAGFSLLQALFVKEHNAVCDMLKERYPDFDDEQLYRHAR 300
           GDGLLEHDEKGIPISGDVRNCWAGFSLLQALFVKEHNAVCDMLKERYPDFDDEQLYRHAR
Sbjct: 241 GDGLLEHDEKGIPISGDVRNCWAGFSLLQALFVKEHNAVCDMLKERYPDFDDEQLYRHAR 300

Query: 301 LVTSAVIAKIHTIDWTVELLKTETLLAGMRINWYGFLGKKFKDSFGHICGPILSGLVGLK 360
           LVTSAVIAKIHTIDWTVELLKTETLLAGMRINWYGFLGKKFKDSFGHICGP+LSGLVGLK
Sbjct: 301 LVTSAVIAKIHTIDWTVELLKTETLLAGMRINWYGFLGKKFKDSFGHICGPVLSGLVGLK 360

Query: 361 KPRDHGIPYSLTEEFVSVYRMHCLLPDKLVIRDLKSTNSDYSDPPVIEEYLFFSLHSLMP 420
           KPRDHGIPYSLTEEFVSVYRMHCLLPDKL IRDL STNSDYSDP VIEE           
Sbjct: 361 KPRDHGIPYSLTEEFVSVYRMHCLLPDKLAIRDLDSTNSDYSDPRVIEE----------- 420

Query: 421 LEMVPMEEMVGKEGEKRLTKLGMEQMLVSMGHQACGALSLWNYPSWMRNLTAHDVEGDDR 480
              VPMEE+VGKEGEKRL K GMEQMLVSMGHQACG+LSLWNYPSWMRNL AHDV+GDDR
Sbjct: 421 ---VPMEELVGKEGEKRLIKFGMEQMLVSMGHQACGSLSLWNYPSWMRNLIAHDVDGDDR 480

Query: 481 PDPVDMAAMEIFRDRERGVARYNEFRRNLLMIPISKWEDLTDDEEVVIALEEVYGNDVEK 540
           PDPVDMAAMEIFRDRERGVARYNEFRRNLLMIPISKWEDLTDDEEVV ALEEVYGNDVEK
Sbjct: 481 PDPVDMAAMEIFRDRERGVARYNEFRRNLLMIPISKWEDLTDDEEVVSALEEVYGNDVEK 540

Query: 541 LDLLVGLHAEKKIKGFAISETAFFIFLLIASRRLEADRFFTTNFNSKTYTEEGLEWVNKT 600
           LDLLVGLHAEKKIKGFAISETAFFIFLLIASRRLEADRFFTTNFNSKTYTEEGLEWVN+T
Sbjct: 541 LDLLVGLHAEKKIKGFAISETAFFIFLLIASRRLEADRFFTTNFNSKTYTEEGLEWVNRT 600

Query: 601 ETLKDVIDRHFPDMTKRWMRCSSAFSVWDSLPNPTNYIPLYLRPAT 647
           ETLKDVIDRHFP+MTKRWMRCSSAFSVWDSLPNPTNYIPLY RPAT
Sbjct: 601 ETLKDVIDRHFPEMTKRWMRCSSAFSVWDSLPNPTNYIPLYFRPAT 632

BLAST of Spg022160 vs. NCBI nr
Match: XP_023515728.1 (alpha-dioxygenase 2 [Cucurbita pepo subsp. pepo] >XP_023515729.1 alpha-dioxygenase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1267.7 bits (3279), Expect = 0.0e+00
Identity = 607/646 (93.96%), Postives = 620/646 (95.98%), Query Frame = 0

Query: 1   MGSSLFSSPFVHPQLQQIVAKMTLLDTLLFYVVHFVDKLGLWHRMPVFMGLAYLGIRRHL 60
           MG+SLFSSPFVHPQLQQIVAKMTLLDTLLFYVVHFVDKLGLWHRMPVFMGLAYLG+RRHL
Sbjct: 1   MGTSLFSSPFVHPQLQQIVAKMTLLDTLLFYVVHFVDKLGLWHRMPVFMGLAYLGLRRHL 60

Query: 61  HQRYNLLHVGAMYGQKYDHQQVCYRTADGSCNHPFDSLVGSQGTFFGRNMPPSTSPYGVL 120
           HQRYNLLHVG++YGQKYDHQQ CYRTADGSCNHP DS+VGSQGTFFGRNMPPSTSPYGVL
Sbjct: 61  HQRYNLLHVGSLYGQKYDHQQFCYRTADGSCNHPSDSVVGSQGTFFGRNMPPSTSPYGVL 120

Query: 121 DPHPTVVATKLLERKNYIDNGKQFNMIACSWIQFMIHDWIDHLEDTKQVELRAPDEVANG 180
           DPHPTVVA+KLLERK YIDNGKQFNMIACSWIQFMIHDWIDHLEDTKQVELR PDEVANG
Sbjct: 121 DPHPTVVASKLLERKKYIDNGKQFNMIACSWIQFMIHDWIDHLEDTKQVELRVPDEVANG 180

Query: 181 CPLKSFKFFRTKVVSTDSPYLKTGTLNTRTPWWDGSVIYGNNEEGMRRVRAFEDGKMKIA 240
           CPLKSFKFFRTKVVST SPYLKTGTLNTRTPWWDGSVIYGNNEEGMRRVRAF+DGKMKIA
Sbjct: 181 CPLKSFKFFRTKVVSTGSPYLKTGTLNTRTPWWDGSVIYGNNEEGMRRVRAFQDGKMKIA 240

Query: 241 GDGLLEHDEKGIPISGDVRNCWAGFSLLQALFVKEHNAVCDMLKERYPDFDDEQLYRHAR 300
           GDGLLEHDEKGIPISGDVRNCWAGFSLLQALFVKEHNAVCDMLKERYPDFDDEQLYRHAR
Sbjct: 241 GDGLLEHDEKGIPISGDVRNCWAGFSLLQALFVKEHNAVCDMLKERYPDFDDEQLYRHAR 300

Query: 301 LVTSAVIAKIHTIDWTVELLKTETLLAGMRINWYGFLGKKFKDSFGHICGPILSGLVGLK 360
           LVTSAVIAKIHTIDWTVELLKTETLLAGMRINWYGFLGKKFKDSFGHICGP+LSGLVGLK
Sbjct: 301 LVTSAVIAKIHTIDWTVELLKTETLLAGMRINWYGFLGKKFKDSFGHICGPVLSGLVGLK 360

Query: 361 KPRDHGIPYSLTEEFVSVYRMHCLLPDKLVIRDLKSTNSDYSDPPVIEEYLFFSLHSLMP 420
           KPRDHGIPYSLTEEFVSVYRMHCLLPDKL IRDL STNSDYSDP VIEE           
Sbjct: 361 KPRDHGIPYSLTEEFVSVYRMHCLLPDKLAIRDLDSTNSDYSDPRVIEE----------- 420

Query: 421 LEMVPMEEMVGKEGEKRLTKLGMEQMLVSMGHQACGALSLWNYPSWMRNLTAHDVEGDDR 480
              VPMEE+VGKEGEKRL K GMEQMLVSMGHQACG+LSLWNYPSWMRNL AHDV+GDDR
Sbjct: 421 ---VPMEELVGKEGEKRLVKFGMEQMLVSMGHQACGSLSLWNYPSWMRNLIAHDVDGDDR 480

Query: 481 PDPVDMAAMEIFRDRERGVARYNEFRRNLLMIPISKWEDLTDDEEVVIALEEVYGNDVEK 540
           PDPVDMAAMEIFRDRERGVARYNEFRRNLLMIPISKWEDLTDDEEVV ALEEVYGNDVEK
Sbjct: 481 PDPVDMAAMEIFRDRERGVARYNEFRRNLLMIPISKWEDLTDDEEVVSALEEVYGNDVEK 540

Query: 541 LDLLVGLHAEKKIKGFAISETAFFIFLLIASRRLEADRFFTTNFNSKTYTEEGLEWVNKT 600
           LDLLVGLHAEKKIKGFAISETAFFIFLLIASRRLEADRFFTTNFNSKTYTEEGLEWVN+T
Sbjct: 541 LDLLVGLHAEKKIKGFAISETAFFIFLLIASRRLEADRFFTTNFNSKTYTEEGLEWVNRT 600

Query: 601 ETLKDVIDRHFPDMTKRWMRCSSAFSVWDSLPNPTNYIPLYLRPAT 647
           ETLKDVIDRHFP+MTKRWMRCSSAFSVWDSLPNPTNYIPLYLRPAT
Sbjct: 601 ETLKDVIDRHFPEMTKRWMRCSSAFSVWDSLPNPTNYIPLYLRPAT 632

BLAST of Spg022160 vs. NCBI nr
Match: XP_022922080.1 (alpha-dioxygenase 2 isoform X1 [Cucurbita moschata] >XP_022922081.1 alpha-dioxygenase 2 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1264.6 bits (3271), Expect = 0.0e+00
Identity = 605/646 (93.65%), Postives = 620/646 (95.98%), Query Frame = 0

Query: 1   MGSSLFSSPFVHPQLQQIVAKMTLLDTLLFYVVHFVDKLGLWHRMPVFMGLAYLGIRRHL 60
           MG+SLFSSPFVHPQLQQIVAKMTLLDTLLFYVVHFVDKLGLWHRMPVFMGLAYLG+RRHL
Sbjct: 1   MGTSLFSSPFVHPQLQQIVAKMTLLDTLLFYVVHFVDKLGLWHRMPVFMGLAYLGLRRHL 60

Query: 61  HQRYNLLHVGAMYGQKYDHQQVCYRTADGSCNHPFDSLVGSQGTFFGRNMPPSTSPYGVL 120
           HQRYNLLHVG++YGQKYDHQQ CYRTADGSCNHP DS+VGSQGTFFGRNMPPSTSPYGVL
Sbjct: 61  HQRYNLLHVGSLYGQKYDHQQFCYRTADGSCNHPSDSVVGSQGTFFGRNMPPSTSPYGVL 120

Query: 121 DPHPTVVATKLLERKNYIDNGKQFNMIACSWIQFMIHDWIDHLEDTKQVELRAPDEVANG 180
           DPHPTVVA+KLLERK YIDNGKQFNMIACSWIQFMIHDWIDHLEDTKQVELR PDEVA+G
Sbjct: 121 DPHPTVVASKLLERKKYIDNGKQFNMIACSWIQFMIHDWIDHLEDTKQVELRVPDEVASG 180

Query: 181 CPLKSFKFFRTKVVSTDSPYLKTGTLNTRTPWWDGSVIYGNNEEGMRRVRAFEDGKMKIA 240
           CPLKSFKFFRTKVVST SPYLKTGTLNTRTPWWDGSVIYGNNEEGMRRVRAF+DGKMKIA
Sbjct: 181 CPLKSFKFFRTKVVSTGSPYLKTGTLNTRTPWWDGSVIYGNNEEGMRRVRAFQDGKMKIA 240

Query: 241 GDGLLEHDEKGIPISGDVRNCWAGFSLLQALFVKEHNAVCDMLKERYPDFDDEQLYRHAR 300
           GDGLLEHDEKGIPISGDVRNCWAGFSLLQALFVKEHNAVCDMLKERYPDFDDEQLYRHAR
Sbjct: 241 GDGLLEHDEKGIPISGDVRNCWAGFSLLQALFVKEHNAVCDMLKERYPDFDDEQLYRHAR 300

Query: 301 LVTSAVIAKIHTIDWTVELLKTETLLAGMRINWYGFLGKKFKDSFGHICGPILSGLVGLK 360
           LVTSAVIAKIHTIDWTVELLKTETLLAGMRINWYGFLGKKFKDSFGHICGP+LSGLVGLK
Sbjct: 301 LVTSAVIAKIHTIDWTVELLKTETLLAGMRINWYGFLGKKFKDSFGHICGPVLSGLVGLK 360

Query: 361 KPRDHGIPYSLTEEFVSVYRMHCLLPDKLVIRDLKSTNSDYSDPPVIEEYLFFSLHSLMP 420
           KPRDHGIPYSLTEEFVSVYRMHCLLPDKL IRDL STNSDYSDP VIEE           
Sbjct: 361 KPRDHGIPYSLTEEFVSVYRMHCLLPDKLAIRDLDSTNSDYSDPRVIEE----------- 420

Query: 421 LEMVPMEEMVGKEGEKRLTKLGMEQMLVSMGHQACGALSLWNYPSWMRNLTAHDVEGDDR 480
              VPMEE+VGKEGEKRL K GMEQMLVSMGHQACG+LSLWNYPSWMRNL AHDV+GDDR
Sbjct: 421 ---VPMEELVGKEGEKRLVKFGMEQMLVSMGHQACGSLSLWNYPSWMRNLIAHDVDGDDR 480

Query: 481 PDPVDMAAMEIFRDRERGVARYNEFRRNLLMIPISKWEDLTDDEEVVIALEEVYGNDVEK 540
           PDPVDMAAMEIFRDRERGVARYNEFRRNLLMIPISKWEDLTDDEEVV ALEEVYGNDVEK
Sbjct: 481 PDPVDMAAMEIFRDRERGVARYNEFRRNLLMIPISKWEDLTDDEEVVSALEEVYGNDVEK 540

Query: 541 LDLLVGLHAEKKIKGFAISETAFFIFLLIASRRLEADRFFTTNFNSKTYTEEGLEWVNKT 600
           LDLLVGLHAEKKIKGFAISETAFFIFLLIASRRLEADRFFTTNFNSKTYTEEGLEWVN+T
Sbjct: 541 LDLLVGLHAEKKIKGFAISETAFFIFLLIASRRLEADRFFTTNFNSKTYTEEGLEWVNRT 600

Query: 601 ETLKDVIDRHFPDMTKRWMRCSSAFSVWDSLPNPTNYIPLYLRPAT 647
           ETLKD+IDRHFP+MTKRWMRCSSAFSVWDSLPNPTNYIPLYLRPAT
Sbjct: 601 ETLKDLIDRHFPEMTKRWMRCSSAFSVWDSLPNPTNYIPLYLRPAT 632

BLAST of Spg022160 vs. NCBI nr
Match: TYK28479.1 (alpha-dioxygenase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1261.5 bits (3263), Expect = 0.0e+00
Identity = 602/646 (93.19%), Postives = 618/646 (95.67%), Query Frame = 0

Query: 1   MGSSLFSSPFVHPQLQQIVAKMTLLDTLLFYVVHFVDKLGLWHRMPVFMGLAYLGIRRHL 60
           MG +LFSSPFVHPQLQQIVAKMTLLDTLLFYVVHFVDKLGLWHRMPVFMGLAYLGIRRHL
Sbjct: 1   MGFALFSSPFVHPQLQQIVAKMTLLDTLLFYVVHFVDKLGLWHRMPVFMGLAYLGIRRHL 60

Query: 61  HQRYNLLHVGAMYGQKYDHQQVCYRTADGSCNHPFDSLVGSQGTFFGRNMPPSTSPYGVL 120
           HQRYNLLHVG+MYGQKYDHQQ CYRTADGSCNHPFDSLVGSQGTFFGRNMPPS+SPYGVL
Sbjct: 61  HQRYNLLHVGSMYGQKYDHQQFCYRTADGSCNHPFDSLVGSQGTFFGRNMPPSSSPYGVL 120

Query: 121 DPHPTVVATKLLERKNYIDNGKQFNMIACSWIQFMIHDWIDHLEDTKQVELRAPDEVANG 180
           DPHPT+VATKLLERK YIDNGKQFNMIACSWIQFMIHDWIDHLEDTKQVEL AP+EVANG
Sbjct: 121 DPHPTLVATKLLERKKYIDNGKQFNMIACSWIQFMIHDWIDHLEDTKQVELTAPEEVANG 180

Query: 181 CPLKSFKFFRTKVVSTDSPYLKTGTLNTRTPWWDGSVIYGNNEEGMRRVRAFEDGKMKIA 240
           CPLKSFKFF TKVVSTDSPYLKTGTLNTRTPWWDGSVIYGNNEEGMRRVR F+DGKMKIA
Sbjct: 181 CPLKSFKFFGTKVVSTDSPYLKTGTLNTRTPWWDGSVIYGNNEEGMRRVRTFQDGKMKIA 240

Query: 241 GDGLLEHDEKGIPISGDVRNCWAGFSLLQALFVKEHNAVCDMLKERYPDFDDEQLYRHAR 300
           GDGLLEHDEKGIPISGDVRNCWAGFSLLQALFVKEHNAVCDMLKERYPDFDDEQLYRHAR
Sbjct: 241 GDGLLEHDEKGIPISGDVRNCWAGFSLLQALFVKEHNAVCDMLKERYPDFDDEQLYRHAR 300

Query: 301 LVTSAVIAKIHTIDWTVELLKTETLLAGMRINWYGFLGKKFKDSFGHICGPILSGLVGLK 360
           LVTSAVIAKIHTIDWTVELLKTETLLAGMRINWYGFLGKKFKD+FGHICGPILSGLVGLK
Sbjct: 301 LVTSAVIAKIHTIDWTVELLKTETLLAGMRINWYGFLGKKFKDTFGHICGPILSGLVGLK 360

Query: 361 KPRDHGIPYSLTEEFVSVYRMHCLLPDKLVIRDLKSTNSDYSDPPVIEEYLFFSLHSLMP 420
           KPRDHGIPYSLTEEFVSVYRMHCLLPDKL+IRDL STNSDYSDPP++EE           
Sbjct: 361 KPRDHGIPYSLTEEFVSVYRMHCLLPDKLIIRDLNSTNSDYSDPPIVEE----------- 420

Query: 421 LEMVPMEEMVGKEGEKRLTKLGMEQMLVSMGHQACGALSLWNYPSWMRNLTAHDVEGDDR 480
              VPME++VGK+GEKRL KLGMEQMLVSMGHQACGALSLWNYPSWMR L AHDV+GDDR
Sbjct: 421 ---VPMEQLVGKDGEKRLAKLGMEQMLVSMGHQACGALSLWNYPSWMRKLIAHDVDGDDR 480

Query: 481 PDPVDMAAMEIFRDRERGVARYNEFRRNLLMIPISKWEDLTDDEEVVIALEEVYGNDVEK 540
           PDPVDMAAMEIFRDRERGVARYNEFRRNLLM PISKWEDLTDD EVV ALEEVYGNDVEK
Sbjct: 481 PDPVDMAAMEIFRDRERGVARYNEFRRNLLMTPISKWEDLTDDNEVVSALEEVYGNDVEK 540

Query: 541 LDLLVGLHAEKKIKGFAISETAFFIFLLIASRRLEADRFFTTNFNSKTYTEEGLEWVNKT 600
           LDLLVGLHAEKKIKGFAISETAFFIFLLIASRRLEADRFFTTN+NSKTYTEEGL+WVNKT
Sbjct: 541 LDLLVGLHAEKKIKGFAISETAFFIFLLIASRRLEADRFFTTNYNSKTYTEEGLDWVNKT 600

Query: 601 ETLKDVIDRHFPDMTKRWMRCSSAFSVWDSLPNPTNYIPLYLRPAT 647
           ETLKDVIDRHFPDMTKRWMRCSSAFSVWDSLPNPTNYIPLYLR AT
Sbjct: 601 ETLKDVIDRHFPDMTKRWMRCSSAFSVWDSLPNPTNYIPLYLRSAT 632

BLAST of Spg022160 vs. NCBI nr
Match: KAG7023315.1 (Alpha-dioxygenase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1260.4 bits (3260), Expect = 0.0e+00
Identity = 603/646 (93.34%), Postives = 618/646 (95.67%), Query Frame = 0

Query: 1   MGSSLFSSPFVHPQLQQIVAKMTLLDTLLFYVVHFVDKLGLWHRMPVFMGLAYLGIRRHL 60
           MG+SLFSSPFVHPQLQQIVAKMTLLDTLLFYVVHFVDKLGLWHRMPVFMGLAYLG+RRHL
Sbjct: 1   MGTSLFSSPFVHPQLQQIVAKMTLLDTLLFYVVHFVDKLGLWHRMPVFMGLAYLGLRRHL 60

Query: 61  HQRYNLLHVGAMYGQKYDHQQVCYRTADGSCNHPFDSLVGSQGTFFGRNMPPSTSPYGVL 120
           HQRYNLLHVG++YGQKYDHQQ CYRTADGSCNHP DS+VGSQGTFFGRNMPPSTSPYGVL
Sbjct: 61  HQRYNLLHVGSLYGQKYDHQQFCYRTADGSCNHPSDSVVGSQGTFFGRNMPPSTSPYGVL 120

Query: 121 DPHPTVVATKLLERKNYIDNGKQFNMIACSWIQFMIHDWIDHLEDTKQVELRAPDEVANG 180
           DPHPTVVA+KLLERK YIDNGKQFNMIACSWIQFMIHDWIDHLEDTKQVELR PDEVA+G
Sbjct: 121 DPHPTVVASKLLERKKYIDNGKQFNMIACSWIQFMIHDWIDHLEDTKQVELRVPDEVASG 180

Query: 181 CPLKSFKFFRTKVVSTDSPYLKTGTLNTRTPWWDGSVIYGNNEEGMRRVRAFEDGKMKIA 240
           CPLKSFKFFRTKVVST SPYLKTGTLNTRTPWWDGSVIYGNNEEGMRRVRAF+DGKMKIA
Sbjct: 181 CPLKSFKFFRTKVVSTGSPYLKTGTLNTRTPWWDGSVIYGNNEEGMRRVRAFQDGKMKIA 240

Query: 241 GDGLLEHDEKGIPISGDVRNCWAGFSLLQALFVKEHNAVCDMLKERYPDFDDEQLYRHAR 300
           GDGLLEHDEKGIPISGDVRNCWAGFSLLQALFVKEHN VCDMLKERYPDFDDEQLYRHAR
Sbjct: 241 GDGLLEHDEKGIPISGDVRNCWAGFSLLQALFVKEHNVVCDMLKERYPDFDDEQLYRHAR 300

Query: 301 LVTSAVIAKIHTIDWTVELLKTETLLAGMRINWYGFLGKKFKDSFGHICGPILSGLVGLK 360
           LVTSAVIAKIHTIDWTVELLKTETLLAGMRINWYGFLGKKFKDSFGHICGP+LSGLVGLK
Sbjct: 301 LVTSAVIAKIHTIDWTVELLKTETLLAGMRINWYGFLGKKFKDSFGHICGPVLSGLVGLK 360

Query: 361 KPRDHGIPYSLTEEFVSVYRMHCLLPDKLVIRDLKSTNSDYSDPPVIEEYLFFSLHSLMP 420
           KPRDHGIPYSLTEEFVSVYRMHCLLPDKL IRDL STNSDYSDP VIEE           
Sbjct: 361 KPRDHGIPYSLTEEFVSVYRMHCLLPDKLAIRDLDSTNSDYSDPRVIEE----------- 420

Query: 421 LEMVPMEEMVGKEGEKRLTKLGMEQMLVSMGHQACGALSLWNYPSWMRNLTAHDVEGDDR 480
              VPME +VGKEGEKRL K GMEQMLVSMGHQACG+LSLWNYPSWMRNL AHDV+GDDR
Sbjct: 421 ---VPMEALVGKEGEKRLVKFGMEQMLVSMGHQACGSLSLWNYPSWMRNLIAHDVDGDDR 480

Query: 481 PDPVDMAAMEIFRDRERGVARYNEFRRNLLMIPISKWEDLTDDEEVVIALEEVYGNDVEK 540
           PDPVDMAAMEIFRDRERGVARYNEFRRNLLMIPISKWEDLTDDEEVV ALEEVYGNDVEK
Sbjct: 481 PDPVDMAAMEIFRDRERGVARYNEFRRNLLMIPISKWEDLTDDEEVVSALEEVYGNDVEK 540

Query: 541 LDLLVGLHAEKKIKGFAISETAFFIFLLIASRRLEADRFFTTNFNSKTYTEEGLEWVNKT 600
           LDLLVGLHAEKKIKGFAISETAFFIFLLIASRRLEADRFFTTNFNSKTYT+EGLEWVN+T
Sbjct: 541 LDLLVGLHAEKKIKGFAISETAFFIFLLIASRRLEADRFFTTNFNSKTYTKEGLEWVNRT 600

Query: 601 ETLKDVIDRHFPDMTKRWMRCSSAFSVWDSLPNPTNYIPLYLRPAT 647
           ETLKDVIDRHFP+MTKRWMRCSSAFSVWDSLPNPTNYIPLYLRPAT
Sbjct: 601 ETLKDVIDRHFPEMTKRWMRCSSAFSVWDSLPNPTNYIPLYLRPAT 632

BLAST of Spg022160 vs. ExPASy Swiss-Prot
Match: Q9C9U3 (Alpha-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=DOX2 PE=2 SV=1)

HSP 1 Score: 1016.5 bits (2627), Expect = 1.3e-295
Identity = 471/645 (73.02%), Postives = 546/645 (84.65%), Query Frame = 0

Query: 1   MGSSLFSSPFVHPQLQQIVAKMTLLDTLLFYVVHFVDKLGLWHRMPVFMGLAYLGIRRHL 60
           MG S  SS F+HPQL  +V+KM+  D  LFY+VH VDKLGLWHR PV +G+AYLG+RRHL
Sbjct: 1   MGFSPSSSWFLHPQLHHVVSKMSYFDAFLFYIVHLVDKLGLWHRFPVLLGVAYLGLRRHL 60

Query: 61  HQRYNLLHVGAMYGQKYDHQQVCYRTADGSCNHPFDSLVGSQGTFFGRNMPPSTSPYGVL 120
           HQRYNL+HVG + GQ YD  + CYRTADG CNHP D+ +GSQG+F GRNMPPSTS YG+L
Sbjct: 61  HQRYNLVHVGPINGQGYDTDEFCYRTADGKCNHPSDNTIGSQGSFIGRNMPPSTSQYGIL 120

Query: 121 DPHPTVVATKLLERKNYIDNGKQFNMIACSWIQFMIHDWIDHLEDTKQVELRAPDEVANG 180
           DPHP+VVATKLL RK +IDNG QFN+IACSWIQFMIHDW+DHLEDT Q+EL AP+EVA+G
Sbjct: 121 DPHPSVVATKLLARKRFIDNGDQFNVIACSWIQFMIHDWVDHLEDTHQIELEAPEEVASG 180

Query: 181 CPLKSFKFFRTKVVSTDSPYLKTGTLNTRTPWWDGSVIYGNNEEGMRRVRAFEDGKMKIA 240
           CPLKSFKF RTK V TD  + K+G +NTRTPWWDGSVIYGN+E GMRRVR F+DGK+KI+
Sbjct: 181 CPLKSFKFLRTKKVPTDDHH-KSGAVNTRTPWWDGSVIYGNDETGMRRVRVFKDGKLKIS 240

Query: 241 GDGLLEHDEKGIPISGDVRNCWAGFSLLQALFVKEHNAVCDMLKERYPDFDDEQLYRHAR 300
           GDGLLE DE+G+PISGD+RN W+GFSLLQALFVKEHN+VCDMLKERYPDFDDE+LYR AR
Sbjct: 241 GDGLLERDERGVPISGDIRNSWSGFSLLQALFVKEHNSVCDMLKERYPDFDDEKLYRTAR 300

Query: 301 LVTSAVIAKIHTIDWTVELLKTETLLAGMRINWYGFLGKKFKDSFGHICGPILSGLVGLK 360
           LVT+AVIAK+HTIDWT+ELLKT+TL AGMRINWYGF GKK KD  G   GP+ SGLVGLK
Sbjct: 301 LVTAAVIAKVHTIDWTIELLKTDTLTAGMRINWYGFFGKKVKDMVGARFGPLFSGLVGLK 360

Query: 361 KPRDHGIPYSLTEEFVSVYRMHCLLPDKLVIRDLKSTNSDYSDPPVIEEYLFFSLHSLMP 420
           KP DHG+PYSLTEEFVSVYRMHCLLP+ L++RD+ S N D  +P +  E           
Sbjct: 361 KPNDHGVPYSLTEEFVSVYRMHCLLPETLILRDMNSENVDKENPAIERE----------- 420

Query: 421 LEMVPMEEMVGKEGEKRLTKLGMEQMLVSMGHQACGALSLWNYPSWMRNLTAHDVEGDDR 480
              +PM E++GK+  ++ +KLG EQ+LVSMGHQ+CGAL+LWNYP+WMRNL A D++G+DR
Sbjct: 421 ---IPMTELIGKKAGEKASKLGFEQLLVSMGHQSCGALTLWNYPNWMRNLVAQDIDGEDR 480

Query: 481 PDPVDMAAMEIFRDRERGVARYNEFRRNLLMIPISKWEDLTDDEEVVIALEEVYGNDVEK 540
           P  +DMAA+EI+RDRERGV RYNEFR+NLLM PISKWE+LTDDEE +  L EVY +D+EK
Sbjct: 481 PHLIDMAALEIYRDRERGVPRYNEFRKNLLMSPISKWEELTDDEEAIKVLREVYEDDIEK 540

Query: 541 LDLLVGLHAEKKIKGFAISETAFFIFLLIASRRLEADRFFTTNFNSKTYTEEGLEWVNKT 600
           LDL VGLHAEKKIKGFAISETAFFIFLL+ASRRLEADRFFTTNFN KTYT+EGLEWVN T
Sbjct: 541 LDLNVGLHAEKKIKGFAISETAFFIFLLVASRRLEADRFFTTNFNEKTYTKEGLEWVNTT 600

Query: 601 ETLKDVIDRHFPDMTKRWMRCSSAFSVWDSLPNPTNYIPLYLRPA 646
           ETLKDVIDRHFP +T +WMRCSSAFSVW S PNP N++PLYLR A
Sbjct: 601 ETLKDVIDRHFPRLTDQWMRCSSAFSVWGSDPNPKNWVPLYLRSA 630

BLAST of Spg022160 vs. ExPASy Swiss-Prot
Match: Q9SGH6 (Alpha-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=DOX1 PE=1 SV=1)

HSP 1 Score: 822.0 bits (2122), Expect = 4.7e-237
Identity = 399/646 (61.76%), Postives = 489/646 (75.70%), Query Frame = 0

Query: 3   SSLFSS---PFVHPQLQQIVAKMTLLDTLLFYVVHFVDKLGLWHRMPVFMGLAYLGIRRH 62
           +SL SS    F+H    +I A+M+LLD  L  +VH VDK+  WH++PVF+GL YL +RRH
Sbjct: 5   TSLISSILLKFIHKDFHEIYARMSLLDRFLLLIVHGVDKMVPWHKLPVFLGLTYLEVRRH 64

Query: 63  LHQRYNLLHVG-AMYGQKYDHQQVCYRTADGSCNHPFDSLVGSQGTFFGRNMPPSTSPYG 122
           LHQ+YNLL+VG    G ++D     YRTADG  N PF+  VGSQ +FFGRN PP      
Sbjct: 65  LHQQYNLLNVGQTPTGIRFDPANYPYRTADGKFNDPFNEGVGSQNSFFGRNCPPVDQKSK 124

Query: 123 VLDPHPTVVATKLLERKNYIDNGKQFNMIACSWIQFMIHDWIDHLEDTKQVELRAPDEVA 182
           +  P P VVATKLL RK +ID GKQFNMIA SWIQFMIHDWIDHLEDT Q+EL AP EVA
Sbjct: 125 LRRPDPMVVATKLLGRKKFIDTGKQFNMIAASWIQFMIHDWIDHLEDTHQIELVAPKEVA 184

Query: 183 NGCPLKSFKFFRTKVVSTDSPYLKTGTLNTRTPWWDGSVIYGNNEEGMRRVRAFEDGKMK 242
           + CPL SF+F +TK V T    +KTG+ N RTPWWD SVIYG+N + + RVR ++DGK+K
Sbjct: 185 SKCPLSSFRFLKTKEVPTGFFEIKTGSQNIRTPWWDSSVIYGSNSKTLDRVRTYKDGKLK 244

Query: 243 IAGD-GLLEHDEKGIPISGDVRNCWAGFSLLQALFVKEHNAVCDMLKERYPDFDDEQLYR 302
           I+ + GLL HDE G+ ISGD+RN WAG S LQALF+KEHNAVCD LK+   D +DE LYR
Sbjct: 245 ISEETGLLLHDEDGLAISGDIRNSWAGVSALQALFIKEHNAVCDALKDEDDDLEDEDLYR 304

Query: 303 HARLVTSAVIAKIHTIDWTVELLKTETLLAGMRINWYGFLGKKFKDSFGHICGPILSGLV 362
           +ARLVTSAV+AKIHTIDWTV+LLKT+TLLAGMR NWYG LGKKFKDSFGH    IL G+V
Sbjct: 305 YARLVTSAVVAKIHTIDWTVQLLKTDTLLAGMRANWYGLLGKKFKDSFGHAGSSILGGVV 364

Query: 363 GLKKPRDHGIPYSLTEEFVSVYRMHCLLPDKLVIRDLKSTNSDYSDPPVIEEYLFFSLHS 422
           G+KKP++HG+PYSLTE+F SVYRMH LLPD+L I D+          P+I+E        
Sbjct: 365 GMKKPQNHGVPYSLTEDFTSVYRMHSLLPDQLHILDIDDVPGTNKSLPLIQE-------- 424

Query: 423 LMPLEMVPMEEMVGKEGEKRLTKLGMEQMLVSMGHQACGALSLWNYPSWMRNLTAHDVEG 482
                 + M +++G++GE+ ++ +G  +++VSMGHQA GAL L NYP W+R++  HD  G
Sbjct: 425 ------ISMRDLIGRKGEETMSHIGFTKLMVSMGHQASGALELMNYPMWLRDIVPHDPNG 484

Query: 483 DDRPDPVDMAAMEIFRDRERGVARYNEFRRNLLMIPISKWEDLTDDEEVVIALEEVYGND 542
             RPD VD+AA+EI+RDRER V RYNEFRR++ MIPI+KWEDLT+DEE +  L++VY  D
Sbjct: 485 QARPDHVDLAALEIYRDRERSVPRYNEFRRSMFMIPITKWEDLTEDEEAIEVLDDVYDGD 544

Query: 543 VEKLDLLVGLHAEKKIKGFAISETAFFIFLLIASRRLEADRFFTTNFNSKTYTEEGLEWV 602
           VE+LDLLVGL AEKKIKGFAISETAF+IFL++A+RRLEADRFFT++FN   YT++GLEWV
Sbjct: 545 VEELDLLVGLMAEKKIKGFAISETAFYIFLIMATRRLEADRFFTSDFNETIYTKKGLEWV 604

Query: 603 NKTETLKDVIDRHFPDMTKRWMRCSSAFSVWDSLPNPTNYIPLYLR 644
           N TE+LKDVIDRH+PDMT +WM   SAFSVWDS P   N IPLYLR
Sbjct: 605 NTTESLKDVIDRHYPDMTDKWMNSESAFSVWDSPPLTKNPIPLYLR 636

BLAST of Spg022160 vs. ExPASy Swiss-Prot
Match: P23219 (Prostaglandin G/H synthase 1 OS=Homo sapiens OX=9606 GN=PTGS1 PE=1 SV=2)

HSP 1 Score: 91.7 bits (226), Expect = 3.4e-17
Identity = 89/353 (25.21%), Postives = 152/353 (43.06%), Query Frame = 0

Query: 218 IYGNNEEGMRRVRAFEDGKMK------------IAGDGLLEHDEKGIP------ISGDVR 277
           IYG+N E   ++R F+DGK+K            +    +L H  +GIP      +  +V 
Sbjct: 232 IYGDNLERQYQLRLFKDGKLKYQVLDGEMYPPSVEEAPVLMHYPRGIPPQSQMAVGQEVF 291

Query: 278 NCWAGFSLLQALFVKEHNAVCDMLKERYPDFDDEQLYRHARLVTSAVIAKIHTIDWTVEL 337
               G  L   L+++EHN VCD+LK  +P + DEQL++  RL+          I  T+++
Sbjct: 292 GLLPGLMLYATLWLREHNRVCDLLKAEHPTWGDEQLFQTTRLI---------LIGETIKI 351

Query: 338 LKTETLLAGMRINWYGFLGKKFKDSFGHICGPILSGLVGLKKPRDHGIPYSLTEEFVSVY 397
           +  E +    +++ Y FL  KF          +L G+    + R       +  EF  +Y
Sbjct: 352 VIEEYV---QQLSGY-FLQLKFDPE-------LLFGVQFQYRNR-------IAMEFNHLY 411

Query: 398 RMHCLLPDKLVIRDLKSTNSDYSDPPVIEEYLFFSLHSLMPLEMVPMEEMVGKEGEKRLT 457
             H L+PD       K  + +YS     E++LF                         L 
Sbjct: 412 HWHPLMPD-----SFKVGSQEYS----YEQFLF---------------------NTSMLV 471

Query: 458 KLGMEQMLVSMGHQACGALSLWNYPSWMRNLTAHDVEGDDRPDPVDMAAMEIFRD-RERG 517
             G+E ++ +   Q  G +         RN+  H +            A+++ R+ RE  
Sbjct: 472 DYGVEALVDAFSRQIAGRI------GGGRNMDHHILH----------VAVDVIRESREMR 510

Query: 518 VARYNEFRRNLLMIPISKWEDLTDDEEVVIALEEVYGNDVEKLDLLVGLHAEK 552
           +  +NE+R+   M P + +++L  ++E+   LEE+YG D++ L+   GL  EK
Sbjct: 532 LQPFNEYRKRFGMKPYTSFQELVGEKEMAAELEELYG-DIDALEFYPGLLLEK 510

BLAST of Spg022160 vs. ExPASy Swiss-Prot
Match: O62664 (Prostaglandin G/H synthase 1 OS=Bos taurus OX=9913 GN=PTGS1 PE=2 SV=2)

HSP 1 Score: 90.5 bits (223), Expect = 7.5e-17
Identity = 89/352 (25.28%), Postives = 149/352 (42.33%), Query Frame = 0

Query: 218 IYGNNEEGMRRVRAFEDGKMK------------IAGDGLLEHDEKGIP------ISGDVR 277
           IYG+N E   ++R F+DGK+K            +    +L H  +GIP      +  +V 
Sbjct: 233 IYGDNLERQYQLRLFKDGKLKYQMLNGEVYPPSVEEAPVLMHYPRGIPPQSQMAVGQEVF 292

Query: 278 NCWAGFSLLQALFVKEHNAVCDMLKERYPDFDDEQLYRHARLVTSAVIAKIHTIDWTVEL 337
               G  +   ++++EHN VCD+LK  +P + DEQL++ ARL+          I  T+++
Sbjct: 293 GLLPGLMVYATIWLREHNRVCDLLKAEHPTWGDEQLFQTARLI---------LIGETIKI 352

Query: 338 LKTETLLAGMRINWYGFLGKKFKDSFGHICGPILSGLVGLKKPRDHGIPYSLTEEFVSVY 397
           +  E +    +++ Y FL  KF          +L G     + R       +  EF  +Y
Sbjct: 353 VIEEYV---QQLSGY-FLQLKFDPE-------LLFGAQFQYRNR-------IAMEFNQLY 412

Query: 398 RMHCLLPDKLVIRDLKSTNSDYSDPPVIEEYLFFSLHSLMPLEMVPMEEMVGKEGEKRLT 457
             H L+PD   +        DYS     E++LF                         L 
Sbjct: 413 HWHPLMPDSFRVGP-----QDYS----YEQFLF---------------------NTSMLV 472

Query: 458 KLGMEQMLVSMGHQACGALSLWNYPSWMRNLTAHDVEGDDRPDPVDMAAMEIFRDRERGV 517
             G+E ++ +   Q  G +         RN+  H +          +A   I   RE  +
Sbjct: 473 DYGVEALVDAFSRQPAGRI------GGGRNIDHHILH---------VAVDVIKESRELRL 511

Query: 518 ARYNEFRRNLLMIPISKWEDLTDDEEVVIALEEVYGNDVEKLDLLVGLHAEK 552
             +NE+R+   M P + +++LT ++E+   LEE+YG D++ L+   GL  EK
Sbjct: 533 QPFNEYRKRFGMKPYTSFQELTGEKEMAAELEELYG-DIDALEFYPGLLLEK 511

BLAST of Spg022160 vs. ExPASy Swiss-Prot
Match: P05979 (Prostaglandin G/H synthase 1 OS=Ovis aries OX=9940 GN=PTGS1 PE=1 SV=3)

HSP 1 Score: 89.0 bits (219), Expect = 2.2e-16
Identity = 89/352 (25.28%), Postives = 148/352 (42.05%), Query Frame = 0

Query: 218 IYGNNEEGMRRVRAFEDGKMK------------IAGDGLLEHDEKGIP------ISGDVR 277
           IYG+N E   ++R F+DGK+K            +    +L H  +GIP      +  +V 
Sbjct: 233 IYGDNLERQYQLRLFKDGKLKYQMLNGEVYPPSVEEAPVLMHYPRGIPPQSQMAVGQEVF 292

Query: 278 NCWAGFSLLQALFVKEHNAVCDMLKERYPDFDDEQLYRHARLVTSAVIAKIHTIDWTVEL 337
               G  L   ++++EHN VCD+LK  +P + DEQL++ ARL+          I  T+++
Sbjct: 293 GLLPGLMLYATIWLREHNRVCDLLKAEHPTWGDEQLFQTARLI---------LIGETIKI 352

Query: 338 LKTETLLAGMRINWYGFLGKKFKDSFGHICGPILSGLVGLKKPRDHGIPYSLTEEFVSVY 397
           +  E +    +++ Y FL  KF          +L G     + R       +  EF  +Y
Sbjct: 353 VIEEYV---QQLSGY-FLQLKFDPE-------LLFGAQFQYRNR-------IAMEFNQLY 412

Query: 398 RMHCLLPDKLVIRDLKSTNSDYSDPPVIEEYLFFSLHSLMPLEMVPMEEMVGKEGEKRLT 457
             H L+PD   +        DYS     E++LF                         L 
Sbjct: 413 HWHPLMPDSFRVGP-----QDYS----YEQFLF---------------------NTSMLV 472

Query: 458 KLGMEQMLVSMGHQACGALSLWNYPSWMRNLTAHDVEGDDRPDPVDMAAMEIFRDRERGV 517
             G+E ++ +   Q  G +         RN+  H +          +A   I   R   +
Sbjct: 473 DYGVEALVDAFSRQPAGRI------GGGRNIDHHILH---------VAVDVIKESRVLRL 511

Query: 518 ARYNEFRRNLLMIPISKWEDLTDDEEVVIALEEVYGNDVEKLDLLVGLHAEK 552
             +NE+R+   M P + +++LT ++E+   LEE+YG D++ L+   GL  EK
Sbjct: 533 QPFNEYRKRFGMKPYTSFQELTGEKEMAAELEELYG-DIDALEFYPGLLLEK 511

BLAST of Spg022160 vs. ExPASy TrEMBL
Match: A0A6J1JDD8 (alpha-dioxygenase 2 OS=Cucurbita maxima OX=3661 GN=LOC111484802 PE=4 SV=1)

HSP 1 Score: 1268.4 bits (3281), Expect = 0.0e+00
Identity = 607/646 (93.96%), Postives = 621/646 (96.13%), Query Frame = 0

Query: 1   MGSSLFSSPFVHPQLQQIVAKMTLLDTLLFYVVHFVDKLGLWHRMPVFMGLAYLGIRRHL 60
           MG+SLFSSPFVHPQLQQIVAKMTLLDTLLFYVVHFVDKLGLWHRMPVFMGLAYLG+RRHL
Sbjct: 1   MGTSLFSSPFVHPQLQQIVAKMTLLDTLLFYVVHFVDKLGLWHRMPVFMGLAYLGLRRHL 60

Query: 61  HQRYNLLHVGAMYGQKYDHQQVCYRTADGSCNHPFDSLVGSQGTFFGRNMPPSTSPYGVL 120
           HQRYNLLHVG++YGQKYDHQQ CYRTADGSCNHP DS+VGSQGTFFGRNMPPSTSPYGVL
Sbjct: 61  HQRYNLLHVGSLYGQKYDHQQFCYRTADGSCNHPSDSVVGSQGTFFGRNMPPSTSPYGVL 120

Query: 121 DPHPTVVATKLLERKNYIDNGKQFNMIACSWIQFMIHDWIDHLEDTKQVELRAPDEVANG 180
           DPHPT+VA+KLLERKNYIDNGKQFNMIACSWIQFMIHDWIDHLEDTKQVELRAPDEVANG
Sbjct: 121 DPHPTMVASKLLERKNYIDNGKQFNMIACSWIQFMIHDWIDHLEDTKQVELRAPDEVANG 180

Query: 181 CPLKSFKFFRTKVVSTDSPYLKTGTLNTRTPWWDGSVIYGNNEEGMRRVRAFEDGKMKIA 240
           CPLKSFKFFRTKVVST SPYLKTGTLNTRTPWWDGSVIYGNNEEGMRRVRAF+DGKMKIA
Sbjct: 181 CPLKSFKFFRTKVVSTGSPYLKTGTLNTRTPWWDGSVIYGNNEEGMRRVRAFQDGKMKIA 240

Query: 241 GDGLLEHDEKGIPISGDVRNCWAGFSLLQALFVKEHNAVCDMLKERYPDFDDEQLYRHAR 300
           GDGLLEHDEKGIPISGDVRNCWAGFSLLQALFVKEHNAVCDMLKERYPDFDDEQLYRHAR
Sbjct: 241 GDGLLEHDEKGIPISGDVRNCWAGFSLLQALFVKEHNAVCDMLKERYPDFDDEQLYRHAR 300

Query: 301 LVTSAVIAKIHTIDWTVELLKTETLLAGMRINWYGFLGKKFKDSFGHICGPILSGLVGLK 360
           LVTSAVIAKIHTIDWTVELLKTETLLAGMRINWYGFLGKKFKDSFGHICGP+LSGLVGLK
Sbjct: 301 LVTSAVIAKIHTIDWTVELLKTETLLAGMRINWYGFLGKKFKDSFGHICGPVLSGLVGLK 360

Query: 361 KPRDHGIPYSLTEEFVSVYRMHCLLPDKLVIRDLKSTNSDYSDPPVIEEYLFFSLHSLMP 420
           KPRDHGIPYSLTEEFVSVYRMHCLLPDKL IRDL STNSDYSDP VIEE           
Sbjct: 361 KPRDHGIPYSLTEEFVSVYRMHCLLPDKLAIRDLDSTNSDYSDPRVIEE----------- 420

Query: 421 LEMVPMEEMVGKEGEKRLTKLGMEQMLVSMGHQACGALSLWNYPSWMRNLTAHDVEGDDR 480
              VPMEE+VGKEGEKRL K GMEQMLVSMGHQACG+LSLWNYPSWMRNL AHDV+GDDR
Sbjct: 421 ---VPMEELVGKEGEKRLIKFGMEQMLVSMGHQACGSLSLWNYPSWMRNLIAHDVDGDDR 480

Query: 481 PDPVDMAAMEIFRDRERGVARYNEFRRNLLMIPISKWEDLTDDEEVVIALEEVYGNDVEK 540
           PDPVDMAAMEIFRDRERGVARYNEFRRNLLMIPISKWEDLTDDEEVV ALEEVYGNDVEK
Sbjct: 481 PDPVDMAAMEIFRDRERGVARYNEFRRNLLMIPISKWEDLTDDEEVVSALEEVYGNDVEK 540

Query: 541 LDLLVGLHAEKKIKGFAISETAFFIFLLIASRRLEADRFFTTNFNSKTYTEEGLEWVNKT 600
           LDLLVGLHAEKKIKGFAISETAFFIFLLIASRRLEADRFFTTNFNSKTYTEEGLEWVN+T
Sbjct: 541 LDLLVGLHAEKKIKGFAISETAFFIFLLIASRRLEADRFFTTNFNSKTYTEEGLEWVNRT 600

Query: 601 ETLKDVIDRHFPDMTKRWMRCSSAFSVWDSLPNPTNYIPLYLRPAT 647
           ETLKDVIDRHFP+MTKRWMRCSSAFSVWDSLPNPTNYIPLY RPAT
Sbjct: 601 ETLKDVIDRHFPEMTKRWMRCSSAFSVWDSLPNPTNYIPLYFRPAT 632

BLAST of Spg022160 vs. ExPASy TrEMBL
Match: A0A6J1E7L4 (alpha-dioxygenase 2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430137 PE=4 SV=1)

HSP 1 Score: 1264.6 bits (3271), Expect = 0.0e+00
Identity = 605/646 (93.65%), Postives = 620/646 (95.98%), Query Frame = 0

Query: 1   MGSSLFSSPFVHPQLQQIVAKMTLLDTLLFYVVHFVDKLGLWHRMPVFMGLAYLGIRRHL 60
           MG+SLFSSPFVHPQLQQIVAKMTLLDTLLFYVVHFVDKLGLWHRMPVFMGLAYLG+RRHL
Sbjct: 1   MGTSLFSSPFVHPQLQQIVAKMTLLDTLLFYVVHFVDKLGLWHRMPVFMGLAYLGLRRHL 60

Query: 61  HQRYNLLHVGAMYGQKYDHQQVCYRTADGSCNHPFDSLVGSQGTFFGRNMPPSTSPYGVL 120
           HQRYNLLHVG++YGQKYDHQQ CYRTADGSCNHP DS+VGSQGTFFGRNMPPSTSPYGVL
Sbjct: 61  HQRYNLLHVGSLYGQKYDHQQFCYRTADGSCNHPSDSVVGSQGTFFGRNMPPSTSPYGVL 120

Query: 121 DPHPTVVATKLLERKNYIDNGKQFNMIACSWIQFMIHDWIDHLEDTKQVELRAPDEVANG 180
           DPHPTVVA+KLLERK YIDNGKQFNMIACSWIQFMIHDWIDHLEDTKQVELR PDEVA+G
Sbjct: 121 DPHPTVVASKLLERKKYIDNGKQFNMIACSWIQFMIHDWIDHLEDTKQVELRVPDEVASG 180

Query: 181 CPLKSFKFFRTKVVSTDSPYLKTGTLNTRTPWWDGSVIYGNNEEGMRRVRAFEDGKMKIA 240
           CPLKSFKFFRTKVVST SPYLKTGTLNTRTPWWDGSVIYGNNEEGMRRVRAF+DGKMKIA
Sbjct: 181 CPLKSFKFFRTKVVSTGSPYLKTGTLNTRTPWWDGSVIYGNNEEGMRRVRAFQDGKMKIA 240

Query: 241 GDGLLEHDEKGIPISGDVRNCWAGFSLLQALFVKEHNAVCDMLKERYPDFDDEQLYRHAR 300
           GDGLLEHDEKGIPISGDVRNCWAGFSLLQALFVKEHNAVCDMLKERYPDFDDEQLYRHAR
Sbjct: 241 GDGLLEHDEKGIPISGDVRNCWAGFSLLQALFVKEHNAVCDMLKERYPDFDDEQLYRHAR 300

Query: 301 LVTSAVIAKIHTIDWTVELLKTETLLAGMRINWYGFLGKKFKDSFGHICGPILSGLVGLK 360
           LVTSAVIAKIHTIDWTVELLKTETLLAGMRINWYGFLGKKFKDSFGHICGP+LSGLVGLK
Sbjct: 301 LVTSAVIAKIHTIDWTVELLKTETLLAGMRINWYGFLGKKFKDSFGHICGPVLSGLVGLK 360

Query: 361 KPRDHGIPYSLTEEFVSVYRMHCLLPDKLVIRDLKSTNSDYSDPPVIEEYLFFSLHSLMP 420
           KPRDHGIPYSLTEEFVSVYRMHCLLPDKL IRDL STNSDYSDP VIEE           
Sbjct: 361 KPRDHGIPYSLTEEFVSVYRMHCLLPDKLAIRDLDSTNSDYSDPRVIEE----------- 420

Query: 421 LEMVPMEEMVGKEGEKRLTKLGMEQMLVSMGHQACGALSLWNYPSWMRNLTAHDVEGDDR 480
              VPMEE+VGKEGEKRL K GMEQMLVSMGHQACG+LSLWNYPSWMRNL AHDV+GDDR
Sbjct: 421 ---VPMEELVGKEGEKRLVKFGMEQMLVSMGHQACGSLSLWNYPSWMRNLIAHDVDGDDR 480

Query: 481 PDPVDMAAMEIFRDRERGVARYNEFRRNLLMIPISKWEDLTDDEEVVIALEEVYGNDVEK 540
           PDPVDMAAMEIFRDRERGVARYNEFRRNLLMIPISKWEDLTDDEEVV ALEEVYGNDVEK
Sbjct: 481 PDPVDMAAMEIFRDRERGVARYNEFRRNLLMIPISKWEDLTDDEEVVSALEEVYGNDVEK 540

Query: 541 LDLLVGLHAEKKIKGFAISETAFFIFLLIASRRLEADRFFTTNFNSKTYTEEGLEWVNKT 600
           LDLLVGLHAEKKIKGFAISETAFFIFLLIASRRLEADRFFTTNFNSKTYTEEGLEWVN+T
Sbjct: 541 LDLLVGLHAEKKIKGFAISETAFFIFLLIASRRLEADRFFTTNFNSKTYTEEGLEWVNRT 600

Query: 601 ETLKDVIDRHFPDMTKRWMRCSSAFSVWDSLPNPTNYIPLYLRPAT 647
           ETLKD+IDRHFP+MTKRWMRCSSAFSVWDSLPNPTNYIPLYLRPAT
Sbjct: 601 ETLKDLIDRHFPEMTKRWMRCSSAFSVWDSLPNPTNYIPLYLRPAT 632

BLAST of Spg022160 vs. ExPASy TrEMBL
Match: A0A5D3DYN1 (Alpha-dioxygenase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G001130 PE=4 SV=1)

HSP 1 Score: 1261.5 bits (3263), Expect = 0.0e+00
Identity = 602/646 (93.19%), Postives = 618/646 (95.67%), Query Frame = 0

Query: 1   MGSSLFSSPFVHPQLQQIVAKMTLLDTLLFYVVHFVDKLGLWHRMPVFMGLAYLGIRRHL 60
           MG +LFSSPFVHPQLQQIVAKMTLLDTLLFYVVHFVDKLGLWHRMPVFMGLAYLGIRRHL
Sbjct: 1   MGFALFSSPFVHPQLQQIVAKMTLLDTLLFYVVHFVDKLGLWHRMPVFMGLAYLGIRRHL 60

Query: 61  HQRYNLLHVGAMYGQKYDHQQVCYRTADGSCNHPFDSLVGSQGTFFGRNMPPSTSPYGVL 120
           HQRYNLLHVG+MYGQKYDHQQ CYRTADGSCNHPFDSLVGSQGTFFGRNMPPS+SPYGVL
Sbjct: 61  HQRYNLLHVGSMYGQKYDHQQFCYRTADGSCNHPFDSLVGSQGTFFGRNMPPSSSPYGVL 120

Query: 121 DPHPTVVATKLLERKNYIDNGKQFNMIACSWIQFMIHDWIDHLEDTKQVELRAPDEVANG 180
           DPHPT+VATKLLERK YIDNGKQFNMIACSWIQFMIHDWIDHLEDTKQVEL AP+EVANG
Sbjct: 121 DPHPTLVATKLLERKKYIDNGKQFNMIACSWIQFMIHDWIDHLEDTKQVELTAPEEVANG 180

Query: 181 CPLKSFKFFRTKVVSTDSPYLKTGTLNTRTPWWDGSVIYGNNEEGMRRVRAFEDGKMKIA 240
           CPLKSFKFF TKVVSTDSPYLKTGTLNTRTPWWDGSVIYGNNEEGMRRVR F+DGKMKIA
Sbjct: 181 CPLKSFKFFGTKVVSTDSPYLKTGTLNTRTPWWDGSVIYGNNEEGMRRVRTFQDGKMKIA 240

Query: 241 GDGLLEHDEKGIPISGDVRNCWAGFSLLQALFVKEHNAVCDMLKERYPDFDDEQLYRHAR 300
           GDGLLEHDEKGIPISGDVRNCWAGFSLLQALFVKEHNAVCDMLKERYPDFDDEQLYRHAR
Sbjct: 241 GDGLLEHDEKGIPISGDVRNCWAGFSLLQALFVKEHNAVCDMLKERYPDFDDEQLYRHAR 300

Query: 301 LVTSAVIAKIHTIDWTVELLKTETLLAGMRINWYGFLGKKFKDSFGHICGPILSGLVGLK 360
           LVTSAVIAKIHTIDWTVELLKTETLLAGMRINWYGFLGKKFKD+FGHICGPILSGLVGLK
Sbjct: 301 LVTSAVIAKIHTIDWTVELLKTETLLAGMRINWYGFLGKKFKDTFGHICGPILSGLVGLK 360

Query: 361 KPRDHGIPYSLTEEFVSVYRMHCLLPDKLVIRDLKSTNSDYSDPPVIEEYLFFSLHSLMP 420
           KPRDHGIPYSLTEEFVSVYRMHCLLPDKL+IRDL STNSDYSDPP++EE           
Sbjct: 361 KPRDHGIPYSLTEEFVSVYRMHCLLPDKLIIRDLNSTNSDYSDPPIVEE----------- 420

Query: 421 LEMVPMEEMVGKEGEKRLTKLGMEQMLVSMGHQACGALSLWNYPSWMRNLTAHDVEGDDR 480
              VPME++VGK+GEKRL KLGMEQMLVSMGHQACGALSLWNYPSWMR L AHDV+GDDR
Sbjct: 421 ---VPMEQLVGKDGEKRLAKLGMEQMLVSMGHQACGALSLWNYPSWMRKLIAHDVDGDDR 480

Query: 481 PDPVDMAAMEIFRDRERGVARYNEFRRNLLMIPISKWEDLTDDEEVVIALEEVYGNDVEK 540
           PDPVDMAAMEIFRDRERGVARYNEFRRNLLM PISKWEDLTDD EVV ALEEVYGNDVEK
Sbjct: 481 PDPVDMAAMEIFRDRERGVARYNEFRRNLLMTPISKWEDLTDDNEVVSALEEVYGNDVEK 540

Query: 541 LDLLVGLHAEKKIKGFAISETAFFIFLLIASRRLEADRFFTTNFNSKTYTEEGLEWVNKT 600
           LDLLVGLHAEKKIKGFAISETAFFIFLLIASRRLEADRFFTTN+NSKTYTEEGL+WVNKT
Sbjct: 541 LDLLVGLHAEKKIKGFAISETAFFIFLLIASRRLEADRFFTTNYNSKTYTEEGLDWVNKT 600

Query: 601 ETLKDVIDRHFPDMTKRWMRCSSAFSVWDSLPNPTNYIPLYLRPAT 647
           ETLKDVIDRHFPDMTKRWMRCSSAFSVWDSLPNPTNYIPLYLR AT
Sbjct: 601 ETLKDVIDRHFPDMTKRWMRCSSAFSVWDSLPNPTNYIPLYLRSAT 632

BLAST of Spg022160 vs. ExPASy TrEMBL
Match: A0A1S3BVR5 (alpha-dioxygenase 2 OS=Cucumis melo OX=3656 GN=LOC103494169 PE=4 SV=1)

HSP 1 Score: 1260.0 bits (3259), Expect = 0.0e+00
Identity = 602/646 (93.19%), Postives = 617/646 (95.51%), Query Frame = 0

Query: 1   MGSSLFSSPFVHPQLQQIVAKMTLLDTLLFYVVHFVDKLGLWHRMPVFMGLAYLGIRRHL 60
           MG  LFSSPFVHP+LQQIVAKMTLLDTLLFYVVHFVDKLGLWHRMPVFMGLAYLGIRRHL
Sbjct: 1   MGFVLFSSPFVHPRLQQIVAKMTLLDTLLFYVVHFVDKLGLWHRMPVFMGLAYLGIRRHL 60

Query: 61  HQRYNLLHVGAMYGQKYDHQQVCYRTADGSCNHPFDSLVGSQGTFFGRNMPPSTSPYGVL 120
           HQRYNLLHVG+MYGQKYDHQQ CYRTADGSCNHPFDSLVGSQGTFFGRNMPPS+SPYGVL
Sbjct: 61  HQRYNLLHVGSMYGQKYDHQQFCYRTADGSCNHPFDSLVGSQGTFFGRNMPPSSSPYGVL 120

Query: 121 DPHPTVVATKLLERKNYIDNGKQFNMIACSWIQFMIHDWIDHLEDTKQVELRAPDEVANG 180
           DPHPT+VATKLLERK YIDNGKQFNMIACSWIQFMIHDWIDHLEDTKQVEL AP+EVANG
Sbjct: 121 DPHPTLVATKLLERKKYIDNGKQFNMIACSWIQFMIHDWIDHLEDTKQVELTAPEEVANG 180

Query: 181 CPLKSFKFFRTKVVSTDSPYLKTGTLNTRTPWWDGSVIYGNNEEGMRRVRAFEDGKMKIA 240
           CPLKSFKFF TKVVSTDSPYLKTGTLNTRTPWWDGSVIYGNNEEGMRRVR F+DGKMKIA
Sbjct: 181 CPLKSFKFFGTKVVSTDSPYLKTGTLNTRTPWWDGSVIYGNNEEGMRRVRTFQDGKMKIA 240

Query: 241 GDGLLEHDEKGIPISGDVRNCWAGFSLLQALFVKEHNAVCDMLKERYPDFDDEQLYRHAR 300
           GDGLLEHDEKGIPISGDVRNCWAGFSLLQALFVKEHNAVCDMLKERYPDFDDEQLYRHAR
Sbjct: 241 GDGLLEHDEKGIPISGDVRNCWAGFSLLQALFVKEHNAVCDMLKERYPDFDDEQLYRHAR 300

Query: 301 LVTSAVIAKIHTIDWTVELLKTETLLAGMRINWYGFLGKKFKDSFGHICGPILSGLVGLK 360
           LVTSAVIAKIHTIDWTVELLKTETLLAGMRINWYGFLGKKFKD+FGHICGPILSGLVGLK
Sbjct: 301 LVTSAVIAKIHTIDWTVELLKTETLLAGMRINWYGFLGKKFKDTFGHICGPILSGLVGLK 360

Query: 361 KPRDHGIPYSLTEEFVSVYRMHCLLPDKLVIRDLKSTNSDYSDPPVIEEYLFFSLHSLMP 420
           KPRDHGIPYSLTEEFVSVYRMHCLLPDKL+IRDL STNSDYSDPP++EE           
Sbjct: 361 KPRDHGIPYSLTEEFVSVYRMHCLLPDKLIIRDLNSTNSDYSDPPIVEE----------- 420

Query: 421 LEMVPMEEMVGKEGEKRLTKLGMEQMLVSMGHQACGALSLWNYPSWMRNLTAHDVEGDDR 480
              VPME++VGK+GEKRL KLGMEQMLVSMGHQACGALSLWNYPSWMR L AHDV+GDDR
Sbjct: 421 ---VPMEQLVGKDGEKRLAKLGMEQMLVSMGHQACGALSLWNYPSWMRKLIAHDVDGDDR 480

Query: 481 PDPVDMAAMEIFRDRERGVARYNEFRRNLLMIPISKWEDLTDDEEVVIALEEVYGNDVEK 540
           PDPVDMAAMEIFRDRERGVARYNEFRRNLLM PISKWEDLTDD EVV ALEEVYGNDVEK
Sbjct: 481 PDPVDMAAMEIFRDRERGVARYNEFRRNLLMTPISKWEDLTDDNEVVSALEEVYGNDVEK 540

Query: 541 LDLLVGLHAEKKIKGFAISETAFFIFLLIASRRLEADRFFTTNFNSKTYTEEGLEWVNKT 600
           LDLLVGLHAEKKIKGFAISETAFFIFLLIASRRLEADRFFTTN+NSKTYTEEGLEWVNKT
Sbjct: 541 LDLLVGLHAEKKIKGFAISETAFFIFLLIASRRLEADRFFTTNYNSKTYTEEGLEWVNKT 600

Query: 601 ETLKDVIDRHFPDMTKRWMRCSSAFSVWDSLPNPTNYIPLYLRPAT 647
           ETLKDVIDRHFPDMTKRWMRCSSAFSVWDSLPNPTNYIPLYLR AT
Sbjct: 601 ETLKDVIDRHFPDMTKRWMRCSSAFSVWDSLPNPTNYIPLYLRSAT 632

BLAST of Spg022160 vs. ExPASy TrEMBL
Match: A0A5A7UWY4 (Alpha-dioxygenase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G004350 PE=4 SV=1)

HSP 1 Score: 1260.0 bits (3259), Expect = 0.0e+00
Identity = 602/646 (93.19%), Postives = 617/646 (95.51%), Query Frame = 0

Query: 1   MGSSLFSSPFVHPQLQQIVAKMTLLDTLLFYVVHFVDKLGLWHRMPVFMGLAYLGIRRHL 60
           MG  LFSSPFVHP+LQQIVAKMTLLDTLLFYVVHFVDKLGLWHRMPVFMGLAYLGIRRHL
Sbjct: 1   MGFVLFSSPFVHPRLQQIVAKMTLLDTLLFYVVHFVDKLGLWHRMPVFMGLAYLGIRRHL 60

Query: 61  HQRYNLLHVGAMYGQKYDHQQVCYRTADGSCNHPFDSLVGSQGTFFGRNMPPSTSPYGVL 120
           HQRYNLLHVG+MYGQKYDHQQ CYRTADGSCNHPFDSLVGSQGTFFGRNMPPS+SPYGVL
Sbjct: 61  HQRYNLLHVGSMYGQKYDHQQFCYRTADGSCNHPFDSLVGSQGTFFGRNMPPSSSPYGVL 120

Query: 121 DPHPTVVATKLLERKNYIDNGKQFNMIACSWIQFMIHDWIDHLEDTKQVELRAPDEVANG 180
           DPHPT+VATKLLERK YIDNGKQFNMIACSWIQFMIHDWIDHLEDTKQVEL AP+EVANG
Sbjct: 121 DPHPTLVATKLLERKKYIDNGKQFNMIACSWIQFMIHDWIDHLEDTKQVELTAPEEVANG 180

Query: 181 CPLKSFKFFRTKVVSTDSPYLKTGTLNTRTPWWDGSVIYGNNEEGMRRVRAFEDGKMKIA 240
           CPLKSFKFF TKVVSTDSPYLKTGTLNTRTPWWDGSVIYGNNEEGMRRVR F+DGKMKIA
Sbjct: 181 CPLKSFKFFGTKVVSTDSPYLKTGTLNTRTPWWDGSVIYGNNEEGMRRVRTFQDGKMKIA 240

Query: 241 GDGLLEHDEKGIPISGDVRNCWAGFSLLQALFVKEHNAVCDMLKERYPDFDDEQLYRHAR 300
           GDGLLEHDEKGIPISGDVRNCWAGFSLLQALFVKEHNAVCDMLKERYPDFDDEQLYRHAR
Sbjct: 241 GDGLLEHDEKGIPISGDVRNCWAGFSLLQALFVKEHNAVCDMLKERYPDFDDEQLYRHAR 300

Query: 301 LVTSAVIAKIHTIDWTVELLKTETLLAGMRINWYGFLGKKFKDSFGHICGPILSGLVGLK 360
           LVTSAVIAKIHTIDWTVELLKTETLLAGMRINWYGFLGKKFKD+FGHICGPILSGLVGLK
Sbjct: 301 LVTSAVIAKIHTIDWTVELLKTETLLAGMRINWYGFLGKKFKDTFGHICGPILSGLVGLK 360

Query: 361 KPRDHGIPYSLTEEFVSVYRMHCLLPDKLVIRDLKSTNSDYSDPPVIEEYLFFSLHSLMP 420
           KPRDHGIPYSLTEEFVSVYRMHCLLPDKL+IRDL STNSDYSDPP++EE           
Sbjct: 361 KPRDHGIPYSLTEEFVSVYRMHCLLPDKLIIRDLNSTNSDYSDPPIVEE----------- 420

Query: 421 LEMVPMEEMVGKEGEKRLTKLGMEQMLVSMGHQACGALSLWNYPSWMRNLTAHDVEGDDR 480
              VPME++VGK+GEKRL KLGMEQMLVSMGHQACGALSLWNYPSWMR L AHDV+GDDR
Sbjct: 421 ---VPMEQLVGKDGEKRLAKLGMEQMLVSMGHQACGALSLWNYPSWMRKLIAHDVDGDDR 480

Query: 481 PDPVDMAAMEIFRDRERGVARYNEFRRNLLMIPISKWEDLTDDEEVVIALEEVYGNDVEK 540
           PDPVDMAAMEIFRDRERGVARYNEFRRNLLM PISKWEDLTDD EVV ALEEVYGNDVEK
Sbjct: 481 PDPVDMAAMEIFRDRERGVARYNEFRRNLLMTPISKWEDLTDDNEVVSALEEVYGNDVEK 540

Query: 541 LDLLVGLHAEKKIKGFAISETAFFIFLLIASRRLEADRFFTTNFNSKTYTEEGLEWVNKT 600
           LDLLVGLHAEKKIKGFAISETAFFIFLLIASRRLEADRFFTTN+NSKTYTEEGLEWVNKT
Sbjct: 541 LDLLVGLHAEKKIKGFAISETAFFIFLLIASRRLEADRFFTTNYNSKTYTEEGLEWVNKT 600

Query: 601 ETLKDVIDRHFPDMTKRWMRCSSAFSVWDSLPNPTNYIPLYLRPAT 647
           ETLKDVIDRHFPDMTKRWMRCSSAFSVWDSLPNPTNYIPLYLR AT
Sbjct: 601 ETLKDVIDRHFPDMTKRWMRCSSAFSVWDSLPNPTNYIPLYLRSAT 632

BLAST of Spg022160 vs. TAIR 10
Match: AT1G73680.1 (alpha dioxygenase )

HSP 1 Score: 1016.5 bits (2627), Expect = 9.2e-297
Identity = 471/645 (73.02%), Postives = 546/645 (84.65%), Query Frame = 0

Query: 1   MGSSLFSSPFVHPQLQQIVAKMTLLDTLLFYVVHFVDKLGLWHRMPVFMGLAYLGIRRHL 60
           MG S  SS F+HPQL  +V+KM+  D  LFY+VH VDKLGLWHR PV +G+AYLG+RRHL
Sbjct: 1   MGFSPSSSWFLHPQLHHVVSKMSYFDAFLFYIVHLVDKLGLWHRFPVLLGVAYLGLRRHL 60

Query: 61  HQRYNLLHVGAMYGQKYDHQQVCYRTADGSCNHPFDSLVGSQGTFFGRNMPPSTSPYGVL 120
           HQRYNL+HVG + GQ YD  + CYRTADG CNHP D+ +GSQG+F GRNMPPSTS YG+L
Sbjct: 61  HQRYNLVHVGPINGQGYDTDEFCYRTADGKCNHPSDNTIGSQGSFIGRNMPPSTSQYGIL 120

Query: 121 DPHPTVVATKLLERKNYIDNGKQFNMIACSWIQFMIHDWIDHLEDTKQVELRAPDEVANG 180
           DPHP+VVATKLL RK +IDNG QFN+IACSWIQFMIHDW+DHLEDT Q+EL AP+EVA+G
Sbjct: 121 DPHPSVVATKLLARKRFIDNGDQFNVIACSWIQFMIHDWVDHLEDTHQIELEAPEEVASG 180

Query: 181 CPLKSFKFFRTKVVSTDSPYLKTGTLNTRTPWWDGSVIYGNNEEGMRRVRAFEDGKMKIA 240
           CPLKSFKF RTK V TD  + K+G +NTRTPWWDGSVIYGN+E GMRRVR F+DGK+KI+
Sbjct: 181 CPLKSFKFLRTKKVPTDDHH-KSGAVNTRTPWWDGSVIYGNDETGMRRVRVFKDGKLKIS 240

Query: 241 GDGLLEHDEKGIPISGDVRNCWAGFSLLQALFVKEHNAVCDMLKERYPDFDDEQLYRHAR 300
           GDGLLE DE+G+PISGD+RN W+GFSLLQALFVKEHN+VCDMLKERYPDFDDE+LYR AR
Sbjct: 241 GDGLLERDERGVPISGDIRNSWSGFSLLQALFVKEHNSVCDMLKERYPDFDDEKLYRTAR 300

Query: 301 LVTSAVIAKIHTIDWTVELLKTETLLAGMRINWYGFLGKKFKDSFGHICGPILSGLVGLK 360
           LVT+AVIAK+HTIDWT+ELLKT+TL AGMRINWYGF GKK KD  G   GP+ SGLVGLK
Sbjct: 301 LVTAAVIAKVHTIDWTIELLKTDTLTAGMRINWYGFFGKKVKDMVGARFGPLFSGLVGLK 360

Query: 361 KPRDHGIPYSLTEEFVSVYRMHCLLPDKLVIRDLKSTNSDYSDPPVIEEYLFFSLHSLMP 420
           KP DHG+PYSLTEEFVSVYRMHCLLP+ L++RD+ S N D  +P +  E           
Sbjct: 361 KPNDHGVPYSLTEEFVSVYRMHCLLPETLILRDMNSENVDKENPAIERE----------- 420

Query: 421 LEMVPMEEMVGKEGEKRLTKLGMEQMLVSMGHQACGALSLWNYPSWMRNLTAHDVEGDDR 480
              +PM E++GK+  ++ +KLG EQ+LVSMGHQ+CGAL+LWNYP+WMRNL A D++G+DR
Sbjct: 421 ---IPMTELIGKKAGEKASKLGFEQLLVSMGHQSCGALTLWNYPNWMRNLVAQDIDGEDR 480

Query: 481 PDPVDMAAMEIFRDRERGVARYNEFRRNLLMIPISKWEDLTDDEEVVIALEEVYGNDVEK 540
           P  +DMAA+EI+RDRERGV RYNEFR+NLLM PISKWE+LTDDEE +  L EVY +D+EK
Sbjct: 481 PHLIDMAALEIYRDRERGVPRYNEFRKNLLMSPISKWEELTDDEEAIKVLREVYEDDIEK 540

Query: 541 LDLLVGLHAEKKIKGFAISETAFFIFLLIASRRLEADRFFTTNFNSKTYTEEGLEWVNKT 600
           LDL VGLHAEKKIKGFAISETAFFIFLL+ASRRLEADRFFTTNFN KTYT+EGLEWVN T
Sbjct: 541 LDLNVGLHAEKKIKGFAISETAFFIFLLVASRRLEADRFFTTNFNEKTYTKEGLEWVNTT 600

Query: 601 ETLKDVIDRHFPDMTKRWMRCSSAFSVWDSLPNPTNYIPLYLRPA 646
           ETLKDVIDRHFP +T +WMRCSSAFSVW S PNP N++PLYLR A
Sbjct: 601 ETLKDVIDRHFPRLTDQWMRCSSAFSVWGSDPNPKNWVPLYLRSA 630

BLAST of Spg022160 vs. TAIR 10
Match: AT1G73680.2 (alpha dioxygenase )

HSP 1 Score: 993.0 bits (2566), Expect = 1.1e-289
Identity = 466/654 (71.25%), Postives = 540/654 (82.57%), Query Frame = 0

Query: 1   MGSSLFSSPFVHPQLQQIVAKMTLLDTLLFYVVHFVDKLGLWHRMPVFMGLAYLGIRRHL 60
           MG S  SS F+HPQL  +V+KM+  D  LFY+VH VDKLGLWHR PV +G+AYLG+RRHL
Sbjct: 1   MGFSPSSSWFLHPQLHHVVSKMSYFDAFLFYIVHLVDKLGLWHRFPVLLGVAYLGLRRHL 60

Query: 61  HQRYNLLHVGAMYGQKYDHQQVCYRTADGSCNHPFDSLVGSQGTFFGRNMPPSTSPYGVL 120
           HQRYNL+HVG + GQ YD  + CYRTADG CNHP D+ +GSQG+F GRNMPPSTS YG  
Sbjct: 61  HQRYNLVHVGPINGQGYDTDEFCYRTADGKCNHPSDNTIGSQGSFIGRNMPPSTSQYGFF 120

Query: 121 DPH---------PTVVATKLLERKNYIDNGKQFNMIACSWIQFMIHDWIDHLEDTKQVEL 180
             +           VVATKLL RK +IDNG QFN+IACSWIQFMIHDW+DHLEDT Q+EL
Sbjct: 121 CQYFFFPENLEKKIVVATKLLARKRFIDNGDQFNVIACSWIQFMIHDWVDHLEDTHQIEL 180

Query: 181 RAPDEVANGCPLKSFKFFRTKVVSTDSPYLKTGTLNTRTPWWDGSVIYGNNEEGMRRVRA 240
            AP+EVA+GCPLKSFKF RTK V TD  + K+G +NTRTPWWDGSVIYGN+E GMRRVR 
Sbjct: 181 EAPEEVASGCPLKSFKFLRTKKVPTDDHH-KSGAVNTRTPWWDGSVIYGNDETGMRRVRV 240

Query: 241 FEDGKMKIAGDGLLEHDEKGIPISGDVRNCWAGFSLLQALFVKEHNAVCDMLKERYPDFD 300
           F+DGK+KI+GDGLLE DE+G+PISGD+RN W+GFSLLQALFVKEHN+VCDMLKERYPDFD
Sbjct: 241 FKDGKLKISGDGLLERDERGVPISGDIRNSWSGFSLLQALFVKEHNSVCDMLKERYPDFD 300

Query: 301 DEQLYRHARLVTSAVIAKIHTIDWTVELLKTETLLAGMRINWYGFLGKKFKDSFGHICGP 360
           DE+LYR ARLVT+AVIAK+HTIDWT+ELLKT+TL AGMRINWYGF GKK KD  G   GP
Sbjct: 301 DEKLYRTARLVTAAVIAKVHTIDWTIELLKTDTLTAGMRINWYGFFGKKVKDMVGARFGP 360

Query: 361 ILSGLVGLKKPRDHGIPYSLTEEFVSVYRMHCLLPDKLVIRDLKSTNSDYSDPPVIEEYL 420
           + SGLVGLKKP DHG+PYSLTEEFVSVYRMHCLLP+ L++RD+ S N D  +P +  E  
Sbjct: 361 LFSGLVGLKKPNDHGVPYSLTEEFVSVYRMHCLLPETLILRDMNSENVDKENPAIERE-- 420

Query: 421 FFSLHSLMPLEMVPMEEMVGKEGEKRLTKLGMEQMLVSMGHQACGALSLWNYPSWMRNLT 480
                       +PM E++GK+  ++ +KLG EQ+LVSMGHQ+CGAL+LWNYP+WMRNL 
Sbjct: 421 ------------IPMTELIGKKAGEKASKLGFEQLLVSMGHQSCGALTLWNYPNWMRNLV 480

Query: 481 AHDVEGDDRPDPVDMAAMEIFRDRERGVARYNEFRRNLLMIPISKWEDLTDDEEVVIALE 540
           A D++G+DRP  +DMAA+EI+RDRERGV RYNEFR+NLLM PISKWE+LTDDEE +  L 
Sbjct: 481 AQDIDGEDRPHLIDMAALEIYRDRERGVPRYNEFRKNLLMSPISKWEELTDDEEAIKVLR 540

Query: 541 EVYGNDVEKLDLLVGLHAEKKIKGFAISETAFFIFLLIASRRLEADRFFTTNFNSKTYTE 600
           EVY +D+EKLDL VGLHAEKKIKGFAISETAFFIFLL+ASRRLEADRFFTTNFN KTYT+
Sbjct: 541 EVYEDDIEKLDLNVGLHAEKKIKGFAISETAFFIFLLVASRRLEADRFFTTNFNEKTYTK 600

Query: 601 EGLEWVNKTETLKDVIDRHFPDMTKRWMRCSSAFSVWDSLPNPTNYIPLYLRPA 646
           EGLEWVN TETLKDVIDRHFP +T +WMRCSSAFSVW S PNP N++PLYLR A
Sbjct: 601 EGLEWVNTTETLKDVIDRHFPRLTDQWMRCSSAFSVWGSDPNPKNWVPLYLRSA 639

BLAST of Spg022160 vs. TAIR 10
Match: AT3G01420.1 (Peroxidase superfamily protein )

HSP 1 Score: 822.0 bits (2122), Expect = 3.3e-238
Identity = 399/646 (61.76%), Postives = 489/646 (75.70%), Query Frame = 0

Query: 3   SSLFSS---PFVHPQLQQIVAKMTLLDTLLFYVVHFVDKLGLWHRMPVFMGLAYLGIRRH 62
           +SL SS    F+H    +I A+M+LLD  L  +VH VDK+  WH++PVF+GL YL +RRH
Sbjct: 5   TSLISSILLKFIHKDFHEIYARMSLLDRFLLLIVHGVDKMVPWHKLPVFLGLTYLEVRRH 64

Query: 63  LHQRYNLLHVG-AMYGQKYDHQQVCYRTADGSCNHPFDSLVGSQGTFFGRNMPPSTSPYG 122
           LHQ+YNLL+VG    G ++D     YRTADG  N PF+  VGSQ +FFGRN PP      
Sbjct: 65  LHQQYNLLNVGQTPTGIRFDPANYPYRTADGKFNDPFNEGVGSQNSFFGRNCPPVDQKSK 124

Query: 123 VLDPHPTVVATKLLERKNYIDNGKQFNMIACSWIQFMIHDWIDHLEDTKQVELRAPDEVA 182
           +  P P VVATKLL RK +ID GKQFNMIA SWIQFMIHDWIDHLEDT Q+EL AP EVA
Sbjct: 125 LRRPDPMVVATKLLGRKKFIDTGKQFNMIAASWIQFMIHDWIDHLEDTHQIELVAPKEVA 184

Query: 183 NGCPLKSFKFFRTKVVSTDSPYLKTGTLNTRTPWWDGSVIYGNNEEGMRRVRAFEDGKMK 242
           + CPL SF+F +TK V T    +KTG+ N RTPWWD SVIYG+N + + RVR ++DGK+K
Sbjct: 185 SKCPLSSFRFLKTKEVPTGFFEIKTGSQNIRTPWWDSSVIYGSNSKTLDRVRTYKDGKLK 244

Query: 243 IAGD-GLLEHDEKGIPISGDVRNCWAGFSLLQALFVKEHNAVCDMLKERYPDFDDEQLYR 302
           I+ + GLL HDE G+ ISGD+RN WAG S LQALF+KEHNAVCD LK+   D +DE LYR
Sbjct: 245 ISEETGLLLHDEDGLAISGDIRNSWAGVSALQALFIKEHNAVCDALKDEDDDLEDEDLYR 304

Query: 303 HARLVTSAVIAKIHTIDWTVELLKTETLLAGMRINWYGFLGKKFKDSFGHICGPILSGLV 362
           +ARLVTSAV+AKIHTIDWTV+LLKT+TLLAGMR NWYG LGKKFKDSFGH    IL G+V
Sbjct: 305 YARLVTSAVVAKIHTIDWTVQLLKTDTLLAGMRANWYGLLGKKFKDSFGHAGSSILGGVV 364

Query: 363 GLKKPRDHGIPYSLTEEFVSVYRMHCLLPDKLVIRDLKSTNSDYSDPPVIEEYLFFSLHS 422
           G+KKP++HG+PYSLTE+F SVYRMH LLPD+L I D+          P+I+E        
Sbjct: 365 GMKKPQNHGVPYSLTEDFTSVYRMHSLLPDQLHILDIDDVPGTNKSLPLIQE-------- 424

Query: 423 LMPLEMVPMEEMVGKEGEKRLTKLGMEQMLVSMGHQACGALSLWNYPSWMRNLTAHDVEG 482
                 + M +++G++GE+ ++ +G  +++VSMGHQA GAL L NYP W+R++  HD  G
Sbjct: 425 ------ISMRDLIGRKGEETMSHIGFTKLMVSMGHQASGALELMNYPMWLRDIVPHDPNG 484

Query: 483 DDRPDPVDMAAMEIFRDRERGVARYNEFRRNLLMIPISKWEDLTDDEEVVIALEEVYGND 542
             RPD VD+AA+EI+RDRER V RYNEFRR++ MIPI+KWEDLT+DEE +  L++VY  D
Sbjct: 485 QARPDHVDLAALEIYRDRERSVPRYNEFRRSMFMIPITKWEDLTEDEEAIEVLDDVYDGD 544

Query: 543 VEKLDLLVGLHAEKKIKGFAISETAFFIFLLIASRRLEADRFFTTNFNSKTYTEEGLEWV 602
           VE+LDLLVGL AEKKIKGFAISETAF+IFL++A+RRLEADRFFT++FN   YT++GLEWV
Sbjct: 545 VEELDLLVGLMAEKKIKGFAISETAFYIFLIMATRRLEADRFFTSDFNETIYTKKGLEWV 604

Query: 603 NKTETLKDVIDRHFPDMTKRWMRCSSAFSVWDSLPNPTNYIPLYLR 644
           N TE+LKDVIDRH+PDMT +WM   SAFSVWDS P   N IPLYLR
Sbjct: 605 NTTESLKDVIDRHYPDMTDKWMNSESAFSVWDSPPLTKNPIPLYLR 636