Spg022154 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg022154
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	scaffold2: 5935301 .. 5937139 (+)
RNA-Seq Expression	Spg022154
Synteny	Spg022154

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTGGAATTAAACCGTTCGGCGCCGTCCAAGACGTGGCTTCTAAAGGGAAAGTGCCAGAGAGATACATCCATAAAGAAAGCGATCGAGGAGCTCCAGATGCACCTTTAATGGAAGCTCCTATAATTGACATCGATCTCCTCTCGTCTTCATCCAATTCCGGACCGGAACTCCAGAAATTCCGACATGGACTTCAGTCATGGGGCTCCTTTCTGGTTAGATTCCAGTGAACTTAAAACCCTAATTTCAATCGGATTGTATGGCGATTTCTTTTAAACCCTGGTGTGTTTGGAACCTCATGATTTTGAAGATTTCAAATCAATTCTGTTGTTTGTTATCACAAAAAATAGAATGATTTGAAATTATATTTTTACGCCAAAAATTTTCTCAACTAAAACCATAGGAGATTGTGAGAGATTTCATAGGATTTAGAACTAAATTACATATTTAATCTTGCCATATTTTAATCTTCTGTTAGTAAATCAATATTAAAAACCTATATTAGTAAACTTCTTACTTTCTTTTATTTTCTTGTTACCATCCAAACACAAAATTTTAAATACATTCGTTTTAAATCTTATTTGTTCCAAGCAAGATAACAAATATAAAATCATTTCTTGAACTTTCCTAATTCCAAACGAAAGTTAATTATTTCAGTGGACTCATCTCGATTCAAGAAAAACGTTTGGACTTCGTAAATTGCTTCAAATCAGACTAAAAAAATGTCGGGATTTTGTTATCCTAATGTTTTGAGAACATGATCAAAAGTTTTCTGGTGTTGTTTCAGGCAATAAATCATGGAATGTCCTCTGAACTTCTGGAAGAAGTTCGCCAAGTAATGAAACAGTTCTTTGCTCTTCCAATGGAAGACAAATTGAAATACTCGAGAGAAGAAGATGACACTGAAGGATATGGCAATTCAATGATTCTCTCCAACAACCAAATTCTGGATTGGACAGATCGTTTGAACCTCACTCTTTATCCAGAACAGAGCCGCCGTTTGAAGTACTGGCCAACAACTCCTCAAAGATTCAGGTAATTTTCTCAAATCTTCATCATTCCCTCAACAGTTTTGTGTGTGATTTGGTTTTTATTATGAGTAATGTACAAGAATCTGTACAAACAGTTGGTTTCCAAACATGTTTTGGATCGAAAATCGAATGTGTTGTGATGAACATTGCAGGGAAGTTATTCATGAGTACACTGAAAATTTGAGGGTGCTATGTGAAAAAATCCTCAAGGCCATGGCAAGGTCATTGGATTTGGATGAGAATAGCTTTGTCAACGAGTTTGTCGAGGGATTTAAACTCGCATATTTCAACTTCTACCCTCGATGTCCGAATCCCGATCTCGTTCTCGGTCTCAAGCCGCACTCCGATGGATCAGCAATCACCATTCTGTTGCAGGACAAGGAAGTAGAAGGTCTTCAGATCTTGAAAGGCAATGAGTGGTTCAATGCTCCAATTGTTCCTGATGCTCTTCTCGTCTTTGTTGGGGATCAAGGAGAGGTAAACACTCTTTACAAAAAGTAGTTATAACTAAATCACTTCAGCTAAAAGCACTTATTCTAAAATCACTTGGGTTGACTTGAAATTTTGTAGATAACAAGTAATGGGATATTCAAGAGTGTAGTTCATAGGGTATTGACAAACACAGAGAGGGAGAGGATTTCATTGGCCACTCTTTACCTTCCACATTCAGAGAAAGAAATTGAACCACTTGAGAAGCTCATCAATGAAACTCGGCCAAGGTTGTACAAGACTGTGAAGAACTATGCTTCCCATTACTATCACTACTACCAGCAAGGCCAGAGGCCAATCGATGCTGCAAGAATCTAG

mRNA sequence

ATGGTTGGAATTAAACCGTTCGGCGCCGTCCAAGACGTGGCTTCTAAAGGGAAAGTGCCAGAGAGATACATCCATAAAGAAAGCGATCGAGGAGCTCCAGATGCACCTTTAATGGAAGCTCCTATAATTGACATCGATCTCCTCTCGTCTTCATCCAATTCCGGACCGGAACTCCAGAAATTCCGACATGGACTTCAGTCATGGGGCTCCTTTCTGGCAATAAATCATGGAATGTCCTCTGAACTTCTGGAAGAAGTTCGCCAAGTAATGAAACAGTTCTTTGCTCTTCCAATGGAAGACAAATTGAAATACTCGAGAGAAGAAGATGACACTGAAGGATATGGCAATTCAATGATTCTCTCCAACAACCAAATTCTGGATTGGACAGATCGTTTGAACCTCACTCTTTATCCAGAACAGAGCCGCCGTTTGAAGTACTGGCCAACAACTCCTCAAAGATTCAGGGAAGTTATTCATGAGTACACTGAAAATTTGAGGGTGCTATGTGAAAAAATCCTCAAGGCCATGGCAAGGTCATTGGATTTGGATGAGAATAGCTTTGTCAACGAGTTTGTCGAGGGATTTAAACTCGCATATTTCAACTTCTACCCTCGATGTCCGAATCCCGATCTCGTTCTCGGTCTCAAGCCGCACTCCGATGGATCAGCAATCACCATTCTGTTGCAGGACAAGGAAGTAGAAGGTCTTCAGATCTTGAAAGGCAATGAGTGGTTCAATGCTCCAATTGTTCCTGATGCTCTTCTCGTCTTTGTTGGGGATCAAGGAGAGATAACAAGTAATGGGATATTCAAGAGTGTAGTTCATAGGGTATTGACAAACACAGAGAGGGAGAGGATTTCATTGGCCACTCTTTACCTTCCACATTCAGAGAAAGAAATTGAACCACTTGAGAAGCTCATCAATGAAACTCGGCCAAGGTTGTACAAGACTGTGAAGAACTATGCTTCCCATTACTATCACTACTACCAGCAAGGCCAGAGGCCAATCGATGCTGCAAGAATCTAG

Coding sequence (CDS)

ATGGTTGGAATTAAACCGTTCGGCGCCGTCCAAGACGTGGCTTCTAAAGGGAAAGTGCCAGAGAGATACATCCATAAAGAAAGCGATCGAGGAGCTCCAGATGCACCTTTAATGGAAGCTCCTATAATTGACATCGATCTCCTCTCGTCTTCATCCAATTCCGGACCGGAACTCCAGAAATTCCGACATGGACTTCAGTCATGGGGCTCCTTTCTGGCAATAAATCATGGAATGTCCTCTGAACTTCTGGAAGAAGTTCGCCAAGTAATGAAACAGTTCTTTGCTCTTCCAATGGAAGACAAATTGAAATACTCGAGAGAAGAAGATGACACTGAAGGATATGGCAATTCAATGATTCTCTCCAACAACCAAATTCTGGATTGGACAGATCGTTTGAACCTCACTCTTTATCCAGAACAGAGCCGCCGTTTGAAGTACTGGCCAACAACTCCTCAAAGATTCAGGGAAGTTATTCATGAGTACACTGAAAATTTGAGGGTGCTATGTGAAAAAATCCTCAAGGCCATGGCAAGGTCATTGGATTTGGATGAGAATAGCTTTGTCAACGAGTTTGTCGAGGGATTTAAACTCGCATATTTCAACTTCTACCCTCGATGTCCGAATCCCGATCTCGTTCTCGGTCTCAAGCCGCACTCCGATGGATCAGCAATCACCATTCTGTTGCAGGACAAGGAAGTAGAAGGTCTTCAGATCTTGAAAGGCAATGAGTGGTTCAATGCTCCAATTGTTCCTGATGCTCTTCTCGTCTTTGTTGGGGATCAAGGAGAGATAACAAGTAATGGGATATTCAAGAGTGTAGTTCATAGGGTATTGACAAACACAGAGAGGGAGAGGATTTCATTGGCCACTCTTTACCTTCCACATTCAGAGAAAGAAATTGAACCACTTGAGAAGCTCATCAATGAAACTCGGCCAAGGTTGTACAAGACTGTGAAGAACTATGCTTCCCATTACTATCACTACTACCAGCAAGGCCAGAGGCCAATCGATGCTGCAAGAATCTAG

Protein sequence

MVGIKPFGAVQDVASKGKVPERYIHKESDRGAPDAPLMEAPIIDIDLLSSSSNSGPELQKFRHGLQSWGSFLAINHGMSSELLEEVRQVMKQFFALPMEDKLKYSREEDDTEGYGNSMILSNNQILDWTDRLNLTLYPEQSRRLKYWPTTPQRFREVIHEYTENLRVLCEKILKAMARSLDLDENSFVNEFVEGFKLAYFNFYPRCPNPDLVLGLKPHSDGSAITILLQDKEVEGLQILKGNEWFNAPIVPDALLVFVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYLPHSEKEIEPLEKLINETRPRLYKTVKNYASHYYHYYQQGQRPIDAARI

Homology

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BLAST of Spg022154 vs. NCBI nr
Match: XP_022134811.1 (uncharacterized protein LOC111006992 [Momordica charantia])

HSP 1 Score: 537.0 bits (1382), Expect = 1.2e-148
Identity = 264/342 (77.19%), Postives = 295/342 (86.26%), Query Frame = 0


Query: 1   MVGIKPFGAVQDVASKGKVPERYIHKESDRGAPDAPLMEAPIIDIDLLSSSSNSGPELQK 60
           M G  P G+VQDVASKG+VPERYIHKE DRGA DAPLMEAP+IDI LLSS SN+GPEL+K
Sbjct: 1   MAGTNPTGSVQDVASKGEVPERYIHKECDRGALDAPLMEAPVIDIGLLSSPSNTGPELEK 60

Query: 61  FRHGLQSWGSFLAINHGMSSELLEEVRQVMKQFFALPMEDKLKYSREEDDTEGYGNSMIL 120
            RHGL SWG F AINHGMS E LEEVRQV K FFALPMEDKLK+SREED  EGYGN MI 
Sbjct: 61  LRHGLHSWGCFQAINHGMSPEFLEEVRQVAKLFFALPMEDKLKHSREEDKMEGYGNDMIF 120

Query: 121 SNNQILDWTDRLNLTLYPEQSRRLKYWPTTPQRFREVIHEYTENLRVLCEKILKAMARSL 180
           SN QILDWTDRL LT+ PE+SRR KYWPT P+RFREV+HEYT N+++L EKILKAMARSL
Sbjct: 121 SNQQILDWTDRLYLTVCPEESRRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMARSL 180

Query: 181 DLDENSFVNEFVEGFKL-AYFNFYPRCPNPDLVLGLKPHSDGSAITILLQDKEVEGLQIL 240
           DLDENSF+N++ +  +L A FNFY RC NP+LVLG+KPH+DGSAITILLQDKEVEGLQ L
Sbjct: 181 DLDENSFLNQYGKRVQLDARFNFYLRCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFL 240

Query: 241 KGNEWFNAPIVPDALLVFVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYLPHSEKE 300
           KGNEW+NAPI+PDALLV VGDQGEITSNGIFKS VHRVLTN+ERERISLA  YLP  +KE
Sbjct: 241 KGNEWWNAPIIPDALLVNVGDQGEITSNGIFKSRVHRVLTNSERERISLAVFYLPDPQKE 300

Query: 301 IEPLEKLINETRPRLYKTVKNYASHYYHYYQQGQRPIDAARI 342
           IEPLEKLINET PRLY+TVKN+   ++ YYQQGQRP +AA+I
Sbjct: 301 IEPLEKLINETHPRLYRTVKNFVGLFFQYYQQGQRPREAAKI 342

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BLAST of Spg022154 vs. NCBI nr
Match: KAA0050082.1 (protein SRG1-like [Cucumis melo var. makuwa])

HSP 1 Score: 533.1 bits (1372), Expect = 1.8e-147
Identity = 262/342 (76.61%), Postives = 295/342 (86.26%), Query Frame = 0


Query: 1   MVGIKPFGAVQDVASKGKVPERYIHKESDRGAPDAPLMEAPIIDIDLLSSSSNSGPELQK 60
           M    P G VQDVASKG+VPERYIHKESDRGA +APLM AP+IDI LLSSSS SGPEL+K
Sbjct: 20  MASTNPSGTVQDVASKGEVPERYIHKESDRGARNAPLMAAPVIDIALLSSSSKSGPELEK 79

Query: 61  FRHGLQSWGSFLAINHGMSSELLEEVRQVMKQFFALPMEDKLKYSREEDDTEGYGNSMIL 120
            RHGLQSWG F AINHGM+SE L+EVRQ+ KQFF L ME+KLKY +EE + EGYGN MIL
Sbjct: 80  LRHGLQSWGCFQAINHGMTSEYLDEVRQLTKQFFGLSMEEKLKYLKEEHEMEGYGNDMIL 139

Query: 121 SNNQILDWTDRLNLTLYPEQSRRLKYWPTTPQRFREVIHEYTENLRVLCEKILKAMARSL 180
           SN QILDWTDRL LT+YP QSRR KYWPT PQRFREV+ EYT N++++ EKILKAMARSL
Sbjct: 140 SNQQILDWTDRLYLTVYPHQSRRFKYWPTNPQRFREVVGEYTTNVKLISEKILKAMARSL 199

Query: 181 DLDENSFVNEFVEGFKL-AYFNFYPRCPNPDLVLGLKPHSDGSAITILLQDKEVEGLQIL 240
           DLDE+SF+N++ E  KL A FNFYPRC NPDLVLG+KPH+DGSAITILLQDKEVEGLQ +
Sbjct: 200 DLDESSFLNQYGEQVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 259

Query: 241 KGNEWFNAPIVPDALLVFVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYLPHSEKE 300
           K NEW+NAPIVPDALLV VGDQ EITSNGIFKS VHRVLTN+ERERISLA  YLP SEKE
Sbjct: 260 KDNEWYNAPIVPDALLVNVGDQVEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 319

Query: 301 IEPLEKLINETRPRLYKTVKNYASHYYHYYQQGQRPIDAARI 342
           IEPLE+LINE++PRLYK+VKN+   Y+ YYQQGQRP++AARI
Sbjct: 320 IEPLEELINESQPRLYKSVKNFVGLYFEYYQQGQRPMEAARI 361

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BLAST of Spg022154 vs. NCBI nr
Match: XP_022960723.1 (protein SRG1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 531.2 bits (1367), Expect = 6.7e-147
Identity = 258/342 (75.44%), Postives = 293/342 (85.67%), Query Frame = 0


Query: 1   MVGIKPFGAVQDVASKGKVPERYIHKESDRGAPDAPLMEAPIIDIDLLSSSSNSGPELQK 60
           M G  P G VQDVASKG+VPERYIHKESDRGA DAPLM AP+ID+ LLSSSS SGPEL+K
Sbjct: 20  MAGTNPSGTVQDVASKGEVPERYIHKESDRGARDAPLMAAPVIDMALLSSSSESGPELEK 79

Query: 61  FRHGLQSWGSFLAINHGMSSELLEEVRQVMKQFFALPMEDKLKYSREEDDTEGYGNSMIL 120
            RHGLQSWG F  +NHGMS+E L+E+R++ KQFF LPME+K KYSREED+ EGYGN MIL
Sbjct: 80  LRHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEKSKYSREEDEIEGYGNDMIL 139

Query: 121 SNNQILDWTDRLNLTLYPEQSRRLKYWPTTPQRFREVIHEYTENLRVLCEKILKAMARSL 180
           SN QILDWTDRL LT+YP++S R KYWPT P+RFR V+HEYT N+++L EKILKAMA SL
Sbjct: 140 SNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRAVLHEYTANVKLLSEKILKAMAISL 199

Query: 181 DLDENSFVNEFVEGFKL-AYFNFYPRCPNPDLVLGLKPHSDGSAITILLQDKEVEGLQIL 240
           DL+E+SF+ ++ E  KL A FNFYPRC NPDLVLG+KPH+DGSAITILLQD+EVEGLQ L
Sbjct: 200 DLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQFL 259

Query: 241 KGNEWFNAPIVPDALLVFVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYLPHSEKE 300
            GNEWFNAPIVP ALL+ VGDQ EITSNGIFKS VHRVLTN+ERERISLA  YLP  EKE
Sbjct: 260 NGNEWFNAPIVPGALLINVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDWEKE 319

Query: 301 IEPLEKLINETRPRLYKTVKNYASHYYHYYQQGQRPIDAARI 342
           IEPLEKLI+ETRPRLYKTVKN+   Y+ YYQQGQRP++AARI
Sbjct: 320 IEPLEKLIDETRPRLYKTVKNFVGLYFQYYQQGQRPMEAARI 361

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BLAST of Spg022154 vs. NCBI nr
Match: XP_023516521.1 (protein SRG1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 528.1 bits (1359), Expect = 5.7e-146
Identity = 258/342 (75.44%), Postives = 293/342 (85.67%), Query Frame = 0


Query: 1   MVGIKPFGAVQDVASKGKVPERYIHKESDRGAPDAPLMEAPIIDIDLLSSSSNSGPELQK 60
           M G  P G VQ VASKG+VPERYIHKESDRGA DAPLM AP+ID+ LLSSSS SGPEL+K
Sbjct: 1   MAGTNPSGTVQGVASKGEVPERYIHKESDRGARDAPLMAAPVIDMALLSSSSKSGPELEK 60

Query: 61  FRHGLQSWGSFLAINHGMSSELLEEVRQVMKQFFALPMEDKLKYSREEDDTEGYGNSMIL 120
            RHGLQSWG F  +NHGMS+E L+EVR++ KQFF LPME+K KYSREE++ EGYGN MIL
Sbjct: 61  LRHGLQSWGCFQVVNHGMSAEFLDEVRRLAKQFFDLPMEEKSKYSREENEIEGYGNDMIL 120

Query: 121 SNNQILDWTDRLNLTLYPEQSRRLKYWPTTPQRFREVIHEYTENLRVLCEKILKAMARSL 180
           SN QILDWTDRL LT+YP++S R KYWPT P+RFREV+HEYT N+++L EKILKAMA SL
Sbjct: 121 SNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMAISL 180

Query: 181 DLDENSFVNEFVEGFKL-AYFNFYPRCPNPDLVLGLKPHSDGSAITILLQDKEVEGLQIL 240
           DL+E+SF+ ++ E  KL A FNFYPRC NPDLVLG+KPH+DGSAITILLQD+EVEGLQ L
Sbjct: 181 DLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQFL 240

Query: 241 KGNEWFNAPIVPDALLVFVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYLPHSEKE 300
            GNEWFNAPIVP ALLV VGDQ EITSNGIFKS VHRVLTN+ERERISLA  YLP  +KE
Sbjct: 241 NGNEWFNAPIVPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDWKKE 300

Query: 301 IEPLEKLINETRPRLYKTVKNYASHYYHYYQQGQRPIDAARI 342
           IEPLEKLI+ETRPRLYKTVKN+   Y+ YYQQGQRP++AARI
Sbjct: 301 IEPLEKLIDETRPRLYKTVKNFVGLYFQYYQQGQRPMEAARI 342

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BLAST of Spg022154 vs. NCBI nr
Match: XP_022987763.1 (probable 2-oxoglutarate-dependent dioxygenase ANS isoform X2 [Cucurbita maxima])

HSP 1 Score: 528.1 bits (1359), Expect = 5.7e-146
Identity = 258/342 (75.44%), Postives = 293/342 (85.67%), Query Frame = 0


Query: 1   MVGIKPFGAVQDVASKGKVPERYIHKESDRGAPDAPLMEAPIIDIDLLSSSSNSGPELQK 60
           M G  P G VQDVASKG+VPERYIH ESDRGA DAPLM AP+ID+ LLSS S SGPEL+K
Sbjct: 1   MAGTNPSGTVQDVASKGEVPERYIHIESDRGALDAPLMAAPVIDMALLSSPSKSGPELEK 60

Query: 61  FRHGLQSWGSFLAINHGMSSELLEEVRQVMKQFFALPMEDKLKYSREEDDTEGYGNSMIL 120
            RHGLQSWG F  +NHGMS+E L+EVR++ KQFF LPME+K KYSREE++ EGYGN MIL
Sbjct: 61  LRHGLQSWGCFQVVNHGMSAEFLDEVRRLAKQFFDLPMEEKSKYSREENEIEGYGNDMIL 120

Query: 121 SNNQILDWTDRLNLTLYPEQSRRLKYWPTTPQRFREVIHEYTENLRVLCEKILKAMARSL 180
           SN QILDWTDRL LT+YP++S R KYWPT P+RFREV+HEYT N+++L EKILKAMA SL
Sbjct: 121 SNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMAISL 180

Query: 181 DLDENSFVNEFVEGFKL-AYFNFYPRCPNPDLVLGLKPHSDGSAITILLQDKEVEGLQIL 240
           DL+E+SF+ ++ E  KL A FNFYPRC NPDLVLG+KPH+DGSAITILLQD+EVEGLQ L
Sbjct: 181 DLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQFL 240

Query: 241 KGNEWFNAPIVPDALLVFVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYLPHSEKE 300
            GNEWFNAPIVP ALLV VGDQ EITSNGIFKS VHRVLTN+ERERISLA  YLP SEKE
Sbjct: 241 NGNEWFNAPIVPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 300

Query: 301 IEPLEKLINETRPRLYKTVKNYASHYYHYYQQGQRPIDAARI 342
           IEPLEKLI+ETRPRLYK+VKN+   Y+ YYQQGQRP++AARI
Sbjct: 301 IEPLEKLIDETRPRLYKSVKNFVGLYFQYYQQGQRPMEAARI 342

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BLAST of Spg022154 vs. ExPASy Swiss-Prot
Match: O80449 (Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 9.0e-62
Identity = 127/322 (39.44%), Postives = 187/322 (58.07%), Query Frame = 0


Query: 5   KPFGAVQDVASKG--KVPERYIHKESDRGAPDAPLMEAPIIDIDLLSSSSNSG-PE-LQK 64
           +P  +VQ ++  G   VP RY+     R   +    +A  I+I +L  +   G PE L+ 
Sbjct: 7   EPIVSVQSLSQTGVPTVPNRYVKPAHQRPVFNTTQSDAG-IEIPVLDMNDVWGKPEGLRL 66

Query: 65  FRHGLQSWGSFLAINHGMSSELLEEVRQVMKQFFALPMEDKLKYSREEDDTEGYGNSMIL 124
            R   + WG F  +NHG++  L+E VR   ++FF LP+E+K KY+   D  EGYG+ + +
Sbjct: 67  VRSACEEWGFFQMVNHGVTHSLMERVRGAWREFFELPLEEKRKYANSPDTYEGYGSRLGV 126

Query: 125 SNNQILDWTDRLNLTLYPEQSRRLKYWPTTPQRFREVIHEYTENLRVLCEKILKAMARSL 184
             +  LDW+D   L   P   R    WP+ P + RE+I +Y E +R LCE++ + ++ SL
Sbjct: 127 VKDAKLDWSDYFFLNYLPSSIRNPSKWPSQPPKIRELIEKYGEEVRKLCERLTETLSESL 186

Query: 185 DLDENSFVNEFVEGFKLA---YFNFYPRCPNPDLVLGLKPHSDGSAITILLQDKEVEGLQ 244
            L  N  +     G K+      NFYP+CP P L LGL  HSD   ITILL D++V GLQ
Sbjct: 187 GLKPNKLMQALGGGDKVGASLRTNFYPKCPQPQLTLGLSSHSDPGGITILLPDEKVAGLQ 246

Query: 245 ILKGNEWFNAPIVPDALLVFVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYLPHSE 304
           + +G+ W     VP+AL+V +GDQ +I SNGI+KSV H+V+ N+  ER+SLA  Y P S+
Sbjct: 247 VRRGDGWVTIKSVPNALIVNIGDQLQILSNGIYKSVEHQVIVNSGMERVSLAFFYNPRSD 306

Query: 305 KEIEPLEKLINETRPRLYKTVK 320
             + P+E+L+   RP LYK ++
Sbjct: 307 IPVGPIEELVTANRPALYKPIR 327

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BLAST of Spg022154 vs. ExPASy Swiss-Prot
Match: Q39224 (Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 5.1e-57
Identity = 114/330 (34.55%), Postives = 187/330 (56.67%), Query Frame = 0


Query: 19  VPERYIHKESDRGAPDAPL---MEAPIIDIDLLSSSSNSGPELQKFRHGLQSWGSFLAIN 78
           VP RY+  + D+   D      +E PIID+  L SS+    E++K     + WG F  +N
Sbjct: 29  VPPRYVRSDQDKTEVDDDFDVKIEIPIIDMKRLCSSTTMDSEVEKLDFACKEWGFFQLVN 88

Query: 79  HGMSSELLEEVRQVMKQFFALPMEDKLKYSREEDDTEGYGNSMILSNNQILDWTDRLNLT 138
           HG+ S  L++V+  ++ FF LPME+K K+ +  D+ EG+G + ++S +Q LDW D    T
Sbjct: 89  HGIDSSFLDKVKSEIQDFFNLPMEEKKKFWQRPDEIEGFGQAFVVSEDQKLDWADLFFHT 148

Query: 139 LYPEQSRRLKYWPTTPQRFREVIHEYTENLRVLCEKILKAMARSLDLDENSFVNEF--VE 198
           + P + R+   +P  P  FR+ +  Y+  ++ + + ++  MAR+L++        F  V+
Sbjct: 149 VQPVELRKPHLFPKLPLPFRDTLEMYSSEVQSVAKILIAKMARALEIKPEELEKLFDDVD 208

Query: 199 GFKLAYFNFYPRCPNPDLVLGLKPHSDGSAITILLQDKEVEGLQILKGNEWFNAPIVPDA 258
             +    N+YP CP PD V+GL PHSD   +T+L+Q  +VEGLQI K  +W     +P+A
Sbjct: 209 SVQSMRMNYYPPCPQPDQVIGLTPHSDSVGLTVLMQVNDVEGLQIKKDGKWVPVKPLPNA 268

Query: 259 LLVFVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYLPHSEKEIEPLEKLINETRPR 318
            +V +GD  EI +NG ++S+ HR + N+E+ER+S+AT +     KE+ P + L+   +  
Sbjct: 269 FIVNIGDVLEIITNGTYRSIEHRGVVNSEKERLSIATFHNVGMYKEVGPAKSLVERQKVA 328

Query: 319 LYK--TVKNYASHYYHYYQQGQRPIDAARI 342
            +K  T+K Y    +     G+  +DA RI
Sbjct: 329 RFKRLTMKEYNDGLFSRTLDGKAYLDALRI 358

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BLAST of Spg022154 vs. ExPASy Swiss-Prot
Match: Q9FFF6 (Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 3.1e-54
Identity = 120/321 (37.38%), Postives = 175/321 (54.52%), Query Frame = 0


Query: 5   KPFGAVQDVASK--GKVPERYIHKESDR--GAPDAP-LMEAPIIDIDLLSSSSNSGPE-- 64
           +P   VQ +A      +P+RYI   S R     DAP     PIID++ L S      +  
Sbjct: 22  EPIVRVQSLAESNLSSLPDRYIKPASLRPTTTEDAPTATNIPIIDLEGLFSEEGLSDDVI 81

Query: 65  LQKFRHGLQSWGSFLAINHGMSSELLEEVRQVMKQFFALPMEDKLKYSREEDDTEGYGNS 124
           + +     + WG F  +NHG+  EL++  R+  ++FF +P+  K  YS      EGYG+ 
Sbjct: 82  MARISEACRGWGFFQVVNHGVKPELMDAARENWREFFHMPVNAKETYSNSPRTYEGYGSR 141

Query: 125 MILSNNQILDWTDRLNLTLYPEQSRRLKYWPTTPQRFREVIHEYTENLRVLCEKILKAMA 184
           + +     LDW+D   L L P   +    WP+ P   REVI EY E L  L  +I++ ++
Sbjct: 142 LGVEKGASLDWSDYYFLHLLPHHLKDFNKWPSFPPTIREVIDEYGEELVKLSGRIMRVLS 201

Query: 185 RSLDLDENSFVNEFVEGFKLA---YFNFYPRCPNPDLVLGLKPHSDGSAITILLQDKEVE 244
            +L L E+ F   F  G  +      N+YP+CP P+L LGL PHSD   +TILL D +V 
Sbjct: 202 TNLGLKEDKFQEAF-GGENIGACLRVNYYPKCPRPELALGLSPHSDPGGMTILLPDDQVF 261

Query: 245 GLQILKGNEWFNAPIVPDALLVFVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYLP 304
           GLQ+ K + W      P A +V +GDQ +I SN  +KSV HRV+ N+++ER+SLA  Y P
Sbjct: 262 GLQVRKDDTWITVKPHPHAFIVNIGDQIQILSNSTYKSVEHRVIVNSDKERVSLAFFYNP 321

Query: 305 HSEKEIEPLEKLINETRPRLY 316
            S+  I+PL++L++   P LY
Sbjct: 322 KSDIPIQPLQELVSTHNPPLY 341

[Close]

BLAST of Spg022154 vs. ExPASy Swiss-Prot
Match: D4N502 (Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 4.0e-54
Identity = 119/317 (37.54%), Postives = 178/317 (56.15%), Query Frame = 0


Query: 9   AVQDVA--SKGKVPERYI----HKESDRGAPDAPLMEAPIIDI-DLLSSSSNSGP-ELQK 68
           +VQ++A  +  ++P RY        ++ GA        P+ID+ +LLS     G  EL K
Sbjct: 17  SVQELAKLTLAEIPSRYTCTGESPLNNIGASVTDDETVPVIDLQNLLSPEPVVGKLELDK 76

Query: 69  FRHGLQSWGSFLAINHGMSSELLEEVRQVMKQFFALPMEDKLKYSREEDDTEGYGNSMIL 128
                + WG F  +NHG+ + L++ ++  +K FF LPM +K KY +++ D EG+G   I 
Sbjct: 77  LHSACKEWGFFQLVNHGVDALLMDNIKSEIKGFFNLPMNEKTKYGQQDGDFEGFGQPYIE 136

Query: 129 SNNQILDWTDRLNLTLYPEQSRRLKYWPTTPQRFREVIHEYTENLRVLCEKILKAMARSL 188
           S +Q LDWT+  ++   P   R+   +P  P  FRE +  Y   ++ L   + + + +SL
Sbjct: 137 SEDQRLDWTEVFSMLSLPLHLRKPHLFPELPLPFRETLESYLSKMKKLSTVVFEMLEKSL 196

Query: 189 DLDE-NSFVNEFVEGFKLAYFNFYPRCPNPDLVLGLKPHSDGSAITILLQDKEVEGLQIL 248
            L E     + F +G +    N+YP CP P+LVLGL  HSD S +TILLQ  EVEGLQI 
Sbjct: 197 QLVEIKGMTDLFEDGLQTMRMNYYPPCPRPELVLGLTSHSDFSGLTILLQLNEVEGLQIR 256

Query: 249 KGNEWFNAPIVPDALLVFVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYLPHSEKE 308
           K   W +   +PDA +V VGD  EI +NGI++SV HR + N+ +ER+S+AT +    E E
Sbjct: 257 KEERWISIKPLPDAFIVNVGDILEIMTNGIYRSVEHRAVVNSTKERLSIATFHDSKLESE 316

Query: 309 IEPLEKLINETRPRLYK 317
           I P+  L+    P L+K
Sbjct: 317 IGPISSLVTPETPALFK 333

[Close]

BLAST of Spg022154 vs. ExPASy Swiss-Prot
Match: Q9SRM3 (Jasmonate-induced oxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=JOX1 PE=1 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 1.0e-52
Identity = 116/322 (36.02%), Postives = 169/322 (52.48%), Query Frame = 0


Query: 5   KPFGAVQDVASKG--KVPERYIHKESDR-------GAPDAPLMEAPIIDIDLLSSSSNSG 64
           +P   VQ +A      +P+RYI   S R         P+   +  PIID+D L S +   
Sbjct: 51  EPIVRVQSLAESNLTSLPDRYIKPPSQRPQTTIIDHQPEVADINIPIIDLDSLFSGNED- 110

Query: 65  PELQKFRHGLQSWGSFLAINHGMSSELLEEVRQVMKQFFALPMEDKLKYSREEDDTEGYG 124
            + ++     + WG F  INHG+  EL++  R+  K FF LP+E K  YS      EGYG
Sbjct: 111 -DKKRISEACREWGFFQVINHGVKPELMDAARETWKSFFNLPVEAKEVYSNSPRTYEGYG 170

Query: 125 NSMILSNNQILDWTDRLNLTLYPEQSRRLKYWPTTPQRFREVIHEYTENLRVLCEKILKA 184
           + + +    ILDW D   L   P   +    WP+ P   RE+  EY + L  L  +++  
Sbjct: 171 SRLGVEKGAILDWNDYYYLHFLPLALKDFNKWPSLPSNIREMNDEYGKELVKLGGRLMTI 230

Query: 185 MARSLDLDENSFVNEF--VEGFKLAYFNFYPRCPNPDLVLGLKPHSDGSAITILLQDKEV 244
           ++ +L L        F   +       N+YP+CP P+L LGL PHSD   +TILL D +V
Sbjct: 231 LSSNLGLRAEQLQEAFGGEDVGACLRVNYYPKCPQPELALGLSPHSDPGGMTILLPDDQV 290

Query: 245 EGLQILKGNEWFNAPIVPDALLVFVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYL 304
            GLQ+  G+ W     +  A +V +GDQ +I SN  +KSV HRV+ N+E+ER+SLA  Y 
Sbjct: 291 VGLQVRHGDTWITVNPLRHAFIVNIGDQIQILSNSKYKSVEHRVIVNSEKERVSLAFFYN 350

Query: 305 PHSEKEIEPLEKLINETRPRLY 316
           P S+  I+P+++L+  T P LY
Sbjct: 351 PKSDIPIQPMQQLVTSTMPPLY 370

[Close]

BLAST of Spg022154 vs. ExPASy TrEMBL
Match: A0A6J1BYU1 (uncharacterized protein LOC111006992 OS=Momordica charantia OX=3673 GN=LOC111006992 PE=4 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 5.9e-149
Identity = 264/342 (77.19%), Postives = 295/342 (86.26%), Query Frame = 0


Query: 1   MVGIKPFGAVQDVASKGKVPERYIHKESDRGAPDAPLMEAPIIDIDLLSSSSNSGPELQK 60
           M G  P G+VQDVASKG+VPERYIHKE DRGA DAPLMEAP+IDI LLSS SN+GPEL+K
Sbjct: 1   MAGTNPTGSVQDVASKGEVPERYIHKECDRGALDAPLMEAPVIDIGLLSSPSNTGPELEK 60

Query: 61  FRHGLQSWGSFLAINHGMSSELLEEVRQVMKQFFALPMEDKLKYSREEDDTEGYGNSMIL 120
            RHGL SWG F AINHGMS E LEEVRQV K FFALPMEDKLK+SREED  EGYGN MI 
Sbjct: 61  LRHGLHSWGCFQAINHGMSPEFLEEVRQVAKLFFALPMEDKLKHSREEDKMEGYGNDMIF 120

Query: 121 SNNQILDWTDRLNLTLYPEQSRRLKYWPTTPQRFREVIHEYTENLRVLCEKILKAMARSL 180
           SN QILDWTDRL LT+ PE+SRR KYWPT P+RFREV+HEYT N+++L EKILKAMARSL
Sbjct: 121 SNQQILDWTDRLYLTVCPEESRRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMARSL 180

Query: 181 DLDENSFVNEFVEGFKL-AYFNFYPRCPNPDLVLGLKPHSDGSAITILLQDKEVEGLQIL 240
           DLDENSF+N++ +  +L A FNFY RC NP+LVLG+KPH+DGSAITILLQDKEVEGLQ L
Sbjct: 181 DLDENSFLNQYGKRVQLDARFNFYLRCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFL 240

Query: 241 KGNEWFNAPIVPDALLVFVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYLPHSEKE 300
           KGNEW+NAPI+PDALLV VGDQGEITSNGIFKS VHRVLTN+ERERISLA  YLP  +KE
Sbjct: 241 KGNEWWNAPIIPDALLVNVGDQGEITSNGIFKSRVHRVLTNSERERISLAVFYLPDPQKE 300

Query: 301 IEPLEKLINETRPRLYKTVKNYASHYYHYYQQGQRPIDAARI 342
           IEPLEKLINET PRLY+TVKN+   ++ YYQQGQRP +AA+I
Sbjct: 301 IEPLEKLINETHPRLYRTVKNFVGLFFQYYQQGQRPREAAKI 342

[Close]

BLAST of Spg022154 vs. ExPASy TrEMBL
Match: A0A5A7U2M0 (Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00660 PE=3 SV=1)

HSP 1 Score: 533.1 bits (1372), Expect = 8.5e-148
Identity = 262/342 (76.61%), Postives = 295/342 (86.26%), Query Frame = 0


Query: 1   MVGIKPFGAVQDVASKGKVPERYIHKESDRGAPDAPLMEAPIIDIDLLSSSSNSGPELQK 60
           M    P G VQDVASKG+VPERYIHKESDRGA +APLM AP+IDI LLSSSS SGPEL+K
Sbjct: 20  MASTNPSGTVQDVASKGEVPERYIHKESDRGARNAPLMAAPVIDIALLSSSSKSGPELEK 79

Query: 61  FRHGLQSWGSFLAINHGMSSELLEEVRQVMKQFFALPMEDKLKYSREEDDTEGYGNSMIL 120
            RHGLQSWG F AINHGM+SE L+EVRQ+ KQFF L ME+KLKY +EE + EGYGN MIL
Sbjct: 80  LRHGLQSWGCFQAINHGMTSEYLDEVRQLTKQFFGLSMEEKLKYLKEEHEMEGYGNDMIL 139

Query: 121 SNNQILDWTDRLNLTLYPEQSRRLKYWPTTPQRFREVIHEYTENLRVLCEKILKAMARSL 180
           SN QILDWTDRL LT+YP QSRR KYWPT PQRFREV+ EYT N++++ EKILKAMARSL
Sbjct: 140 SNQQILDWTDRLYLTVYPHQSRRFKYWPTNPQRFREVVGEYTTNVKLISEKILKAMARSL 199

Query: 181 DLDENSFVNEFVEGFKL-AYFNFYPRCPNPDLVLGLKPHSDGSAITILLQDKEVEGLQIL 240
           DLDE+SF+N++ E  KL A FNFYPRC NPDLVLG+KPH+DGSAITILLQDKEVEGLQ +
Sbjct: 200 DLDESSFLNQYGEQVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 259

Query: 241 KGNEWFNAPIVPDALLVFVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYLPHSEKE 300
           K NEW+NAPIVPDALLV VGDQ EITSNGIFKS VHRVLTN+ERERISLA  YLP SEKE
Sbjct: 260 KDNEWYNAPIVPDALLVNVGDQVEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 319

Query: 301 IEPLEKLINETRPRLYKTVKNYASHYYHYYQQGQRPIDAARI 342
           IEPLE+LINE++PRLYK+VKN+   Y+ YYQQGQRP++AARI
Sbjct: 320 IEPLEELINESQPRLYKSVKNFVGLYFEYYQQGQRPMEAARI 361

[Close]

BLAST of Spg022154 vs. ExPASy TrEMBL
Match: A0A6J1H9T6 (protein SRG1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111461440 PE=3 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 3.2e-147
Identity = 258/342 (75.44%), Postives = 293/342 (85.67%), Query Frame = 0


Query: 1   MVGIKPFGAVQDVASKGKVPERYIHKESDRGAPDAPLMEAPIIDIDLLSSSSNSGPELQK 60
           M G  P G VQDVASKG+VPERYIHKESDRGA DAPLM AP+ID+ LLSSSS SGPEL+K
Sbjct: 20  MAGTNPSGTVQDVASKGEVPERYIHKESDRGARDAPLMAAPVIDMALLSSSSESGPELEK 79

Query: 61  FRHGLQSWGSFLAINHGMSSELLEEVRQVMKQFFALPMEDKLKYSREEDDTEGYGNSMIL 120
            RHGLQSWG F  +NHGMS+E L+E+R++ KQFF LPME+K KYSREED+ EGYGN MIL
Sbjct: 80  LRHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEKSKYSREEDEIEGYGNDMIL 139

Query: 121 SNNQILDWTDRLNLTLYPEQSRRLKYWPTTPQRFREVIHEYTENLRVLCEKILKAMARSL 180
           SN QILDWTDRL LT+YP++S R KYWPT P+RFR V+HEYT N+++L EKILKAMA SL
Sbjct: 140 SNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRAVLHEYTANVKLLSEKILKAMAISL 199

Query: 181 DLDENSFVNEFVEGFKL-AYFNFYPRCPNPDLVLGLKPHSDGSAITILLQDKEVEGLQIL 240
           DL+E+SF+ ++ E  KL A FNFYPRC NPDLVLG+KPH+DGSAITILLQD+EVEGLQ L
Sbjct: 200 DLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQFL 259

Query: 241 KGNEWFNAPIVPDALLVFVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYLPHSEKE 300
            GNEWFNAPIVP ALL+ VGDQ EITSNGIFKS VHRVLTN+ERERISLA  YLP  EKE
Sbjct: 260 NGNEWFNAPIVPGALLINVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDWEKE 319

Query: 301 IEPLEKLINETRPRLYKTVKNYASHYYHYYQQGQRPIDAARI 342
           IEPLEKLI+ETRPRLYKTVKN+   Y+ YYQQGQRP++AARI
Sbjct: 320 IEPLEKLIDETRPRLYKTVKNFVGLYFQYYQQGQRPMEAARI 361

[Close]

BLAST of Spg022154 vs. ExPASy TrEMBL
Match: A0A6J1JKD6 (probable 2-oxoglutarate-dependent dioxygenase ANS isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485215 PE=3 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 2.7e-146
Identity = 258/342 (75.44%), Postives = 293/342 (85.67%), Query Frame = 0


Query: 1   MVGIKPFGAVQDVASKGKVPERYIHKESDRGAPDAPLMEAPIIDIDLLSSSSNSGPELQK 60
           M G  P G VQDVASKG+VPERYIH ESDRGA DAPLM AP+ID+ LLSS S SGPEL+K
Sbjct: 1   MAGTNPSGTVQDVASKGEVPERYIHIESDRGALDAPLMAAPVIDMALLSSPSKSGPELEK 60

Query: 61  FRHGLQSWGSFLAINHGMSSELLEEVRQVMKQFFALPMEDKLKYSREEDDTEGYGNSMIL 120
            RHGLQSWG F  +NHGMS+E L+EVR++ KQFF LPME+K KYSREE++ EGYGN MIL
Sbjct: 61  LRHGLQSWGCFQVVNHGMSAEFLDEVRRLAKQFFDLPMEEKSKYSREENEIEGYGNDMIL 120

Query: 121 SNNQILDWTDRLNLTLYPEQSRRLKYWPTTPQRFREVIHEYTENLRVLCEKILKAMARSL 180
           SN QILDWTDRL LT+YP++S R KYWPT P+RFREV+HEYT N+++L EKILKAMA SL
Sbjct: 121 SNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMAISL 180

Query: 181 DLDENSFVNEFVEGFKL-AYFNFYPRCPNPDLVLGLKPHSDGSAITILLQDKEVEGLQIL 240
           DL+E+SF+ ++ E  KL A FNFYPRC NPDLVLG+KPH+DGSAITILLQD+EVEGLQ L
Sbjct: 181 DLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQFL 240

Query: 241 KGNEWFNAPIVPDALLVFVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYLPHSEKE 300
            GNEWFNAPIVP ALLV VGDQ EITSNGIFKS VHRVLTN+ERERISLA  YLP SEKE
Sbjct: 241 NGNEWFNAPIVPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 300

Query: 301 IEPLEKLINETRPRLYKTVKNYASHYYHYYQQGQRPIDAARI 342
           IEPLEKLI+ETRPRLYK+VKN+   Y+ YYQQGQRP++AARI
Sbjct: 301 IEPLEKLIDETRPRLYKSVKNFVGLYFQYYQQGQRPMEAARI 342

[Close]

BLAST of Spg022154 vs. ExPASy TrEMBL
Match: A0A5D3CIZ6 (Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold367G00230 PE=3 SV=1)

HSP 1 Score: 526.6 bits (1355), Expect = 8.0e-146
Identity = 259/342 (75.73%), Postives = 293/342 (85.67%), Query Frame = 0


Query: 1   MVGIKPFGAVQDVASKGKVPERYIHKESDRGAPDAPLMEAPIIDIDLLSSSSNSGPELQK 60
           M    P G VQDVASKG+VPERYIHKESDRGA +APLM AP+IDI LLSSSS SGPEL+K
Sbjct: 20  MASTNPSGTVQDVASKGEVPERYIHKESDRGARNAPLMAAPVIDIALLSSSSKSGPELEK 79

Query: 61  FRHGLQSWGSFLAINHGMSSELLEEVRQVMKQFFALPMEDKLKYSREEDDTEGYGNSMIL 120
            RHGLQSWG F AINHGM+SE L+EVRQ+ KQFF L ME+KLKY +EE + EGYGN MIL
Sbjct: 80  LRHGLQSWGCFQAINHGMTSEYLDEVRQLTKQFFGLSMEEKLKYLKEEHEMEGYGNDMIL 139

Query: 121 SNNQILDWTDRLNLTLYPEQSRRLKYWPTTPQRFREVIHEYTENLRVLCEKILKAMARSL 180
           SN QILDWTDRL LT+YP QSRR KYWP  PQRFREV+ EYT N++++ EKILKAMARSL
Sbjct: 140 SNQQILDWTDRLYLTVYPHQSRRFKYWPINPQRFREVVGEYTTNVKLISEKILKAMARSL 199

Query: 181 DLDENSFVNEFVEGFKL-AYFNFYPRCPNPDLVLGLKPHSDGSAITILLQDKEVEGLQIL 240
           DLDE+SF+N++ E  KL A FNFYPRC NPDLVLG+KPH+DGSAITILLQDKEVEGLQ +
Sbjct: 200 DLDESSFLNQYGEQVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 259

Query: 241 KGNEWFNAPIVPDALLVFVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYLPHSEKE 300
           K NEW+NAPIV DALLV VGDQ EITSNGIFKS VHRVLTN+ERERISLA  YLP SEKE
Sbjct: 260 KDNEWYNAPIVTDALLVNVGDQVEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 319

Query: 301 IEPLEKLINETRPRLYKTVKNYASHYYHYYQQGQRPIDAARI 342
           IEPLE+LINE++PRLYK+VKN+   Y+ YYQQG+RP++AARI
Sbjct: 320 IEPLEELINESQPRLYKSVKNFVGLYFEYYQQGERPMEAARI 361

[Close]

BLAST of Spg022154 vs. TAIR 10
Match: AT5G20400.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 366.7 bits (940), Expect = 2.0e-101
Identity = 180/343 (52.48%), Postives = 243/343 (70.85%), Query Frame = 0


Query: 7   FGAVQDVASKGK-VPERYIHKESDRGAPD-----APLMEAPIIDIDLLSSSSNSG-PELQ 66
           F  VQ+V + G+ +PERY+H  +  G         P M+ P ID++LL SSS +G  EL 
Sbjct: 6   FKTVQEVVAAGEGLPERYLHAPTGDGEVQPLNAAVPEMDIPAIDLNLLLSSSEAGQQELS 65

Query: 67  KFRHGLQSWGSFLAINHGMSSELLEEVRQVMKQFFALPMEDKLKYSREEDDTEGYGNSMI 126
           K    L +WG    +NHG++   L+++ ++ K+FFALP E+K K +RE D  +GYGN MI
Sbjct: 66  KLHSALSTWGVVQVMNHGITKAFLDKIYKLTKEFFALPTEEKQKCAREIDSIQGYGNDMI 125

Query: 127 LSNNQILDWTDRLNLTLYPEQSRRLKYWPTTPQRFREVIHEYTENLRVLCEKILKAMARS 186
           L ++Q+LDW DRL +T YPE  R+L +WP  P  FRE +HEYT   R++ E+  KAMARS
Sbjct: 126 LWDDQVLDWIDRLYITTYPEDQRQLNFWPEVPLGFRETLHEYTMKQRIVIEQFFKAMARS 185

Query: 187 LDLDENSFVNEFVEGFKL-AYFNFYPRCPNPDLVLGLKPHSDGSAITILLQDKEVEGLQI 246
           L+L+ENSF++ + E   L   FN YP CP+PD V+G+KPH+DGSAIT+LL DK+V GLQ 
Sbjct: 186 LELEENSFLDMYGESATLDTRFNMYPPCPSPDKVIGVKPHADGSAITLLLPDKDVGGLQF 245

Query: 247 LKGNEWFNAPIVPDALLVFVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYLPHSEK 306
            K  +W+ APIVPD +L+ VGDQ EI SNGI+KS VHRV+TN E+ERIS+AT  +P ++K
Sbjct: 246 QKDGKWYKAPIVPDTILINVGDQMEIMSNGIYKSPVHRVVTNREKERISVATFCIPGADK 305

Query: 307 EIEPLEKLINETRPRLYKTVKNYASHYYHYYQQGQRPIDAARI 342
           EI+P+ +L++E RPRLYKTVK Y   Y+ YYQQG+RPI+AA I
Sbjct: 306 EIQPVNELVSEARPRLYKTVKKYVELYFKYYQQGRRPIEAALI 348

[Close]

BLAST of Spg022154 vs. TAIR 10
Match: AT1G49390.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 361.3 bits (926), Expect = 8.5e-100
Identity = 181/343 (52.77%), Postives = 237/343 (69.10%), Query Frame = 0


Query: 7   FGAVQDVASKGK-VPERYIHKESDRGAPD-----APLMEAPIIDIDLLSSSSNSG-PELQ 66
           F  VQ+V + G+ +PERY+H  +  G         P M+ P ID+ LL SSS  G  E++
Sbjct: 6   FKTVQEVVAAGQGLPERYLHAPTGEGESQPLNGAVPEMDIPAIDLSLLFSSSVDGQEEMK 65

Query: 67  KFRHGLQSWGSFLAINHGMSSELLEEVRQVMKQFFALPMEDKLKYSREEDDTEGYGNSMI 126
           K    L +WG    +NHG++   L+++ ++ KQFFALP E+K K +RE  + +GYGN MI
Sbjct: 66  KLHSALSTWGVVQVMNHGITEAFLDKIYKLTKQFFALPTEEKHKCARETGNIQGYGNDMI 125

Query: 127 LSNNQILDWTDRLNLTLYPEQSRRLKYWPTTPQRFREVIHEYTENLRVLCEKILKAMARS 186
           LS+NQ+LDW DRL LT YPE  R+LK+WP  P  F E + EYT   RVL EK  KAMARS
Sbjct: 126 LSDNQVLDWIDRLFLTTYPEDKRQLKFWPQVPVGFSETLDEYTMKQRVLIEKFFKAMARS 185

Query: 187 LDLDENSFVNEFVEGFKL-AYFNFYPRCPNPDLVLGLKPHSDGSAITILLQDKEVEGLQI 246
           L+L+EN F+  + E   + + FNF+P CP PD V+G+KPH+DGSAIT+LL DK+VEGLQ 
Sbjct: 186 LELEENCFLEMYGENAVMNSRFNFFPPCPRPDKVIGIKPHADGSAITLLLPDKDVEGLQF 245

Query: 247 LKGNEWFNAPIVPDALLVFVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYLPHSEK 306
           LK  +W+ APIVPD +L+ +GDQ EI SNGI+KS VHRV+TN E+ERIS+AT  +P  +K
Sbjct: 246 LKDGKWYKAPIVPDTILITLGDQMEIMSNGIYKSPVHRVVTNREKERISVATFCVPGLDK 305

Query: 307 EIEPLEKLINETRPRLYKTVKNYASHYYHYYQQGQRPIDAARI 342
           EI P + L+ E RPRLYKTV  Y   +Y YYQQG+R I+AA I
Sbjct: 306 EIHPADGLVTEARPRLYKTVTKYVDLHYKYYQQGRRTIEAALI 348

[Close]

BLAST of Spg022154 vs. TAIR 10
Match: AT5G54000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 340.9 bits (873), Expect = 1.2e-93
Identity = 170/341 (49.85%), Postives = 230/341 (67.45%), Query Frame = 0


Query: 10  VQDVASKG-KVPERYIHKESDRGAPDAPL------MEAPIIDIDLLSSSSNSG-PELQKF 69
           VQ+V + G K+PERY++  +  G  D P       M+  IID++LL SSS+ G  EL K 
Sbjct: 9   VQEVVAAGEKLPERYLYTPTGDGEGDQPFNGLLPEMKISIIDLNLLFSSSDDGREELSKL 68

Query: 70  RHGLQSWGSFLAINHGMSSELLEEVRQVMKQFFALPMEDKLKYSREEDDTEGYGNSMILS 129
              + +WG    +NHG+S  LL+++ ++ KQFF LP ++K KY+RE    +G+GN MILS
Sbjct: 69  HSAISTWGVVQVMNHGISEALLDKIHELTKQFFVLPTKEKQKYAREISSFQGFGNDMILS 128

Query: 130 NNQILDWTDRLNLTLYPEQSRRLKYWPTTPQRFREVIHEYTENLRVLCEKILKAMARSLD 189
           ++Q+LDW DRL L  YPE  R+LK+WP  P  FRE +HEYT   +++ EK  KA+ARSL+
Sbjct: 129 DDQVLDWVDRLYLITYPEDQRQLKFWPENPSGFRETLHEYTMKQQLVVEKFFKALARSLE 188

Query: 190 LDENSFVNEFVEGFKL-AYFNFYPRCPNPDLVLGLKPHSDGSAITILLQDKEVEGLQILK 249
           L++N F+    E   L   FN YP CP PD VLGLKPHSDGSA T++L DK VEGLQ LK
Sbjct: 189 LEDNCFLEMHGENATLETRFNIYPPCPRPDKVLGLKPHSDGSAFTLILPDKNVEGLQFLK 248

Query: 250 GNEWFNAPIVPDALLVFVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYLPHSEKEI 309
             +W+ A I+P  +L+ VGD  E+ SNGI+KS VHRV+ N ++ERI +AT      +KEI
Sbjct: 249 DGKWYKASILPHTILINVGDTMEVMSNGIYKSPVHRVVLNGKKERIYVATFCNADEDKEI 308

Query: 310 EPLEKLINETRPRLYKTVKNYASHYYHYYQQGQRPIDAARI 342
           +PL  L++E RPRLYK VK    +++ YYQQG+RPI+AA I
Sbjct: 309 QPLNGLVSEARPRLYKAVKKSEKNFFDYYQQGRRPIEAAMI 349

[Close]

BLAST of Spg022154 vs. TAIR 10
Match: AT5G20550.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 335.9 bits (860), Expect = 3.8e-92
Identity = 172/344 (50.00%), Postives = 233/344 (67.73%), Query Frame = 0


Query: 7   FGAVQDVASKGK-VPERYIHKES--DRGA---PDAPLMEAPIIDIDLLSSSSNSG-PELQ 66
           F  VQ+V + G+ +PERY+   +  D G       P+M+ P ID+ LL S S+ G  EL 
Sbjct: 6   FKTVQEVVAAGEGIPERYLQPPAVDDNGQHLNAAVPVMDIPAIDLSLLLSPSDDGREELS 65

Query: 67  KFRHGLQSWGSFLAINHGMSSELLEEVRQVMKQFFALPMEDKLKYSREEDDTEGYGNSMI 126
           K    L +WG    INHG++  LL+++ ++ K+F ALP E+K KY+RE    +GYGN MI
Sbjct: 66  KLHSALSTWGVVQVINHGITKALLDKIYKLTKEFCALPSEEKQKYAREIGSIQGYGNDMI 125

Query: 127 LSNNQILDWTDRLNLTLYPEQSRRLKYWPTTPQRFREVIHEYTENLRVLCEKILKAMARS 186
           L ++Q+LDW DRL +T YPE  R+LK+WP  P  FRE +HEYT    ++  ++ KAMA S
Sbjct: 126 LWDDQVLDWIDRLYITTYPEDQRQLKFWPDVPVGFRETLHEYTMKQHLVFNQVFKAMAIS 185

Query: 187 LDLDENSFVNEFVEGFKL-AYFNFYPRCPNPDLVLGLKPHSDGSAITILLQDKEVEGLQI 246
           L+L+EN F++   E   +   FN YP CP PD V+G++PH+D SA T+LL DK VEGLQ 
Sbjct: 186 LELEENCFLDMCGENATMDTRFNMYPPCPRPDKVIGVRPHADKSAFTLLLPDKNVEGLQF 245

Query: 247 LKGNEWFNAPIV-PDALLVFVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYLPHSE 306
           LK  +W+ AP+V  D +L+ VGDQ EI SNGI+KS VHRV+TNTE+ERIS+AT  +P ++
Sbjct: 246 LKDGKWYKAPVVASDTILINVGDQMEIMSNGIYKSPVHRVVTNTEKERISVATFCIPGAD 305

Query: 307 KEIEPLEKLINETRPRLYKTVKNYASHYYHYYQQGQRPIDAARI 342
           KEI+P++ L++E RPRLYK VKNY      YY QGQRPI A+ I
Sbjct: 306 KEIQPVDGLVSEARPRLYKPVKNYVDLLNKYYIQGQRPIAASLI 349

[Close]

BLAST of Spg022154 vs. TAIR 10
Match: AT3G21420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 271.6 bits (693), Expect = 8.9e-73
Identity = 144/352 (40.91%), Postives = 210/352 (59.66%), Query Frame = 0


Query: 8   GAVQDV-----ASKGKVPERYIHKESDRGAPDAPLM------EAPIIDIDLLSSSSNSG- 67
           G + DV     +   KVPER+I +E +RG   + L       + P+ID+  LS   N   
Sbjct: 12  GKIDDVQELIKSKPNKVPERFIREEYERGVVVSSLKTHHLHHQIPVIDLSKLSKPDNDDF 71

Query: 68  -PELQKFRHGLQSWGSFLAINHGMSSELLEEVRQVMKQFFALPMEDKLKYSREEDDTEGY 127
             E+ K     + WG F  INHG+  E++E++ +V  +FF +P+E+K KY  E    +GY
Sbjct: 72  FFEILKLSQACEDWGFFQVINHGIEVEVVEDIEEVASEFFDMPLEEKKKYPMEPGTVQGY 131

Query: 128 GNSMILSNNQILDWTDRLNLTLYPEQSRRLKYWPTTPQRFREVIHEYTENLRVLCEKILK 187
           G + I S +Q LDW +   L ++P Q R  K WP+ P RF E +  Y++ +R LC+++LK
Sbjct: 132 GQAFIFSEDQKLDWCNMFALGVHPPQIRNPKLWPSKPARFSESLEGYSKEIRELCKRLLK 191

Query: 188 AMARSLDLDENSFVNEFVEGFKLAYFNFYPRCPNPDLVLGLKPHSDGSAITILLQDK-EV 247
            +A SL L E  F   F E  +    N+YP C +PDLVLGL PHSDGSA+T+L Q K   
Sbjct: 192 YIAISLGLKEERFEEMFGEAVQAVRMNYYPPCSSPDLVLGLSPHSDGSALTVLQQSKNSC 251

Query: 248 EGLQILKGNEWFNAPIVPDALLVFVGDQGEITSNGIFKSVVHRVLTNTERERISLATLYL 307
            GLQILK N W     +P+AL++ +GD  E+ SNG +KSV HR +TN E+ER+++ T Y 
Sbjct: 252 VGLQILKDNTWVPVKPLPNALVINIGDTIEVLSNGKYKSVEHRAVTNREKERLTIVTFYA 311

Query: 308 PHSEKEIEPLEKLI-NETRPRLYKTVKNYASHYYHYYQ---QGQRPIDAARI 342
           P+ E EIEP+ +L+ +ET P  Y++  N+  + YHY     QG++ +D A+I
Sbjct: 312 PNYEVEIEPMSELVDDETNPCKYRSY-NHGDYSYHYVSNKLQGKKSLDFAKI 362

The following BLAST results are available for this feature:

BLAST of Spg022154 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134811.1	1.2e-148	77.19	uncharacterized protein LOC111006992 [Momordica charantia]	[more]
KAA0050082.1	1.8e-147	76.61	protein SRG1-like [Cucumis melo var. makuwa]	[more]
XP_022960723.1	6.7e-147	75.44	protein SRG1-like isoform X1 [Cucurbita moschata]	[more]
XP_023516521.1	5.7e-146	75.44	protein SRG1-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022987763.1	5.7e-146	75.44	probable 2-oxoglutarate-dependent dioxygenase ANS isoform X2 [Cucurbita maxima]	[more]

BLAST of Spg022154 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O80449	9.0e-62	39.44	Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1	[more]
Q39224	5.1e-57	34.55	Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1	[more]
Q9FFF6	3.1e-54	37.38	Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1	[more]
D4N502	4.0e-54	37.54	Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1	[more]
Q9SRM3	1.0e-52	36.02	Jasmonate-induced oxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=JOX1 PE=1 SV=1	[more]

BLAST of Spg022154 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BYU1	5.9e-149	77.19	uncharacterized protein LOC111006992 OS=Momordica charantia OX=3673 GN=LOC111006...	[more]
A0A5A7U2M0	8.5e-148	76.61	Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00...	[more]
A0A6J1H9T6	3.2e-147	75.44	protein SRG1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111461440 PE=3 ...	[more]
A0A6J1JKD6	2.7e-146	75.44	probable 2-oxoglutarate-dependent dioxygenase ANS isoform X2 OS=Cucurbita maxima...	[more]
A0A5D3CIZ6	8.0e-146	75.73	Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold367G00...	[more]

BLAST of Spg022154 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20400.1	2.0e-101	52.48	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G49390.1	8.5e-100	52.77	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G54000.1	1.2e-93	49.85	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G20550.1	3.8e-92	50.00	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G21420.1	8.9e-73	40.91	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 199..292 e-value: 6.1E-22 score: 78.0
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 9..333 e-value: 2.1E-89 score: 302.1
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 41..148 e-value: 1.5E-22 score: 80.6
None	No IPR available	PANTHER	PTHR47991	OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE	coord: 9..337
None	No IPR available	PANTHER	PTHR47991:SF63	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN	coord: 9..337
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 14..321
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 194..294 score: 12.185434

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg022154.1	Spg022154.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:1901576	organic substance biosynthetic process
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen