Spg022152 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg022152 vs. NCBI nr
Match: TYJ98237.1 (putative ATP-dependent helicase YprA isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1696.0 bits (4391), Expect = 0.0e+00
Identity = 878/1071 (81.98%), Postives = 938/1071 (87.58%), Query Frame = 0

Query: 1    MEESEREIGIKTLTGESLTVSISGDRTIEDLKLLLRRNFPSATISPNFHLFFKGSKLKPQ 60
            MEESEREI IKTLTGES+TVS+SG+ TI+DLKLLLRRNFPSATISPNFHLFFKG+KLKPQ
Sbjct: 1    MEESEREIRIKTLTGESMTVSVSGNSTIDDLKLLLRRNFPSATISPNFHLFFKGTKLKPQ 60

Query: 61   SQISACRIDNGEFLVLIPFNKKESSKSLLRDQYEQGSSVSGGSSISQFADSAWSDMVQDL 120
            SQISAC ID+GEFLVLIPFNKKESSKS LRDQYE   SVS G+SISQFADSAWSDMVQDL
Sbjct: 61   SQISACCIDHGEFLVLIPFNKKESSKSRLRDQYELEFSVSSGTSISQFADSAWSDMVQDL 120

Query: 121  SYLHDCSTRGREDNGSERERGNSEVGGVDAELVATRSTSSSSSKAKGRKGFVYNGLKGNL 180
            SYLH CS  GRE+  SE ER +SEVG VDAEL A  STSS SSKAKG+ GF  +GL G+L
Sbjct: 121  SYLHGCSVEGREETVSESERASSEVGEVDAELEACCSTSSLSSKAKGKLGFGNDGLNGSL 180

Query: 181  DDVLRNLLSSPAEGGLSERTCENFVKFLESVDCLSDPRNGKCMLANQVSSRSGNKRAPNR 240
            DDVLRN   SP EG L+E+TCE+F+KFLESVDCL+DPRNG CMLA Q +SRSGNKRAPN 
Sbjct: 181  DDVLRNFSLSPTEGFLNEQTCESFIKFLESVDCLTDPRNGNCMLAKQANSRSGNKRAPNS 240

Query: 241  TCGSSCLCPGWLKKIMKAFAFLNVFSMFLQLQEENMTVSRLEQAMDLLQKHGITLCMEDM 300
            T GSSCLCP WLKKIMKAF+FLNVFSMFLQLQEE MTVSRLEQAMD LQK  I  CMED+
Sbjct: 241  TRGSSCLCPTWLKKIMKAFSFLNVFSMFLQLQEEIMTVSRLEQAMDQLQKRRIMFCMEDI 300

Query: 301  KHLSLLCPKAVHFASGSLEDTGDDTLIIIISLTEQNGRWKDDNTACKSFKAPTDVTLLER 360
             +LS LCPKAVHFASG +EDT    LIIII LTE+NGR K D     +      + LL R
Sbjct: 301  HNLSRLCPKAVHFASGIMEDTRVHKLIIIIYLTEKNGRPKKD---IDNTDGNATIYLLLR 360

Query: 361  RERSFKIFL-----------LEAIKSHMLRHGSRSEICVPFSLEDLITSKESAVDGNEAK 420
                   FL           L  IK  +L+HGS  E+CVPFSLE LITS E+ VDG+E K
Sbjct: 361  IPSYAPAFLNFNIQLGIVVSLLEIKMGLLKHGSGREMCVPFSLEALITSNETDVDGSETK 420

Query: 421  RAKKSETASLSSNSDRMQCHDTSKLLPENMVEHLVNGVGSEGQIVHIEDIAARKANYVEI 480
            RAKKS+TAS SS S+R++CHDTSKLLPENMVEHL   VG EGQIVH+ DI ARKANYVEI
Sbjct: 421  RAKKSDTASSSSQSERIRCHDTSKLLPENMVEHLKKSVGPEGQIVHVNDILARKANYVEI 480

Query: 481  PEELSNNVVSALKCIGVAKLYSHQAQSIEASLAGKNVAVATMTSSGKSLCYNLPVLESMS 540
            P+ELSN+VVSALKCIGVAKLYSHQA+SIEASL+GK+VAVATMTSSGKSLCYNLPVLE+MS
Sbjct: 481  PKELSNSVVSALKCIGVAKLYSHQARSIEASLSGKHVAVATMTSSGKSLCYNLPVLEAMS 540

Query: 541  QNVSSCALYLFPTKALAQDQLRSLLVMMKGFNDNVDIGVYDGDTSQADRIFLRDNARLLI 600
            Q+VSSCALYLFPTKALAQDQLRSLLVMMKGF  N++IGVYDGDTSQADRI LRDNARLLI
Sbjct: 541  QDVSSCALYLFPTKALAQDQLRSLLVMMKGFIHNLNIGVYDGDTSQADRILLRDNARLLI 600

Query: 601  TNPDMLHLSILPHHRQFSRILSNLRFIVIDEAHTYKGAFGCHTALILRRLCRLCCHVYGS 660
            TNPDMLH+SILPHHRQFSRILSNLRFIVIDEAHTYKGAFGCHTALILRRL R+C HVYGS
Sbjct: 601  TNPDMLHVSILPHHRQFSRILSNLRFIVIDEAHTYKGAFGCHTALILRRLRRICSHVYGS 660

Query: 661  DPSFIFCTATSANPRQHCMELGNLSSLELIEIDGSPSARKLFILWNPIMVLSLAQLWTLQ 720
            DPSFIFCTATSANPR+HCMELGNLSSLELI+ DGSPSARKLF+LWNP+MVL +AQLWTLQ
Sbjct: 661  DPSFIFCTATSANPREHCMELGNLSSLELIDNDGSPSARKLFLLWNPVMVLFVAQLWTLQ 720

Query: 721  GSLQKWFSMDFAALLFVKLVNFVNLFYATRRRELLKESAPHLVQSVCAYRAGYTAEDRRR 780
            GSL KWFSMDFAALLFVKL NFV+LFYATRRRE+LKESAPHLVQSVCAYRAGYTAEDRRR
Sbjct: 721  GSLPKWFSMDFAALLFVKLANFVSLFYATRRREILKESAPHLVQSVCAYRAGYTAEDRRR 780

Query: 781  IESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFP----------GRAGRREKTSLSV 840
            IESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFP          GRAGRREKTSLSV
Sbjct: 781  IESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQAGRAGRREKTSLSV 840

Query: 841  YVAFEGPLDQYFMKHPEKLFGSPIECCHIDAENQQVLEQHLLCAAYEHPVNVVYDQKFFG 900
            YVAFEGPLDQYFMKHPEKLFGSPIECCHIDAENQQVLEQHLLCAAYEHPV V YDQ FFG
Sbjct: 841  YVAFEGPLDQYFMKHPEKLFGSPIECCHIDAENQQVLEQHLLCAAYEHPVCVAYDQSFFG 900

Query: 901  PGLNTALISLKSRGDLIPEPSCGSSKSIWNYIGQEKMPSRLVSIRAIETERYKVVDQRQN 960
            PGLN AL+SLK+RGDLIPEPSCGSSKSIWNYIGQEKMPSR VSIRAIE ERYKVVDQR+N
Sbjct: 901  PGLNNALMSLKNRGDLIPEPSCGSSKSIWNYIGQEKMPSRSVSIRAIEAERYKVVDQRRN 960

Query: 961  EVLEEIEESTAFF-------QVYEGAVYLHQGRTYLVKSLNLSTMLAFCEEADLKYYTKT 1020
            EVLEEIEES AFF       QVYEGAVY+HQGRTYLVKSLNLSTMLAFCEEADLKYYTKT
Sbjct: 961  EVLEEIEESKAFFQVFGYVVQVYEGAVYMHQGRTYLVKSLNLSTMLAFCEEADLKYYTKT 1020

Query: 1021 RDFTDVHVIGAKQAVWIPVPQSIKEEVKRKNFDFRAGLHAASHALLNVVPL 1044
            RD+TD+HVIGA+QAVWIPVPQSIKEEVKRKNFDFRAGLHAASHALLNVVPL
Sbjct: 1021 RDYTDIHVIGAEQAVWIPVPQSIKEEVKRKNFDFRAGLHAASHALLNVVPL 1068

BLAST of Spg022152 vs. NCBI nr
Match: XP_038878311.1 (uncharacterized ATP-dependent helicase YprA isoform X5 [Benincasa hispida])

HSP 1 Score: 1691.0 bits (4378), Expect = 0.0e+00
Identity = 891/1138 (78.30%), Postives = 950/1138 (83.48%), Query Frame = 0

Query: 1    MEESEREIGIKTLTGESLTVSISGDRTIEDLKLLLRRNFPSATISPNFHLFFKGSKLKPQ 60
            MEES REI IKTLTGESLTVSISG+RTI+DLKLLLRRNFPSATISPNFHLFFKG+KLKPQ
Sbjct: 1    MEESVREIEIKTLTGESLTVSISGNRTIDDLKLLLRRNFPSATISPNFHLFFKGTKLKPQ 60

Query: 61   SQISACRIDNGEFLVLIPFNKKESSKSLLRDQYEQGSSVSGGSSISQFADSAWSDMVQDL 120
            SQISACRID+GEFLVLIPFNKKESSKS LRDQYEQGSSVS GSSISQFADSAWSDMVQDL
Sbjct: 61   SQISACRIDHGEFLVLIPFNKKESSKSRLRDQYEQGSSVSCGSSISQFADSAWSDMVQDL 120

Query: 121  SYLHDCSTRGREDNGSERERGNSEVGGVDAELVATRSTSSSSSKAKGRKGFVYNGLKGNL 180
            SYLH CS   RE+N  E ERG+SEVGGVDAELVAT STSS SSK+KG+KGF Y+ LKGNL
Sbjct: 121  SYLHGCSDERREENRPESERGSSEVGGVDAELVATCSTSSFSSKSKGKKGFAYDDLKGNL 180

Query: 181  DDVLRNLLSSPAEGGLSERTCENFVKFLESVDCLSDPRNGKCMLANQVSSRSGNKRAPNR 240
            DDVLR   SSP+EG L+E+TCE+F+K+LESVDCLSDPRNG CMLA Q +SRSGNKRAPN 
Sbjct: 181  DDVLRKFSSSPSEGFLNEQTCESFIKYLESVDCLSDPRNGNCMLAKQANSRSGNKRAPNS 240

Query: 241  TCGSSCLCPGWLKKIMKAFAFLNVFSMFLQLQEENMTVSRLEQAMDLLQKHGITLCMEDM 300
            TCGSSCLCP WLKK MKAF+FLNVF+MFLQLQEE MTVSRLEQAMD L+K GITLCMED+
Sbjct: 241  TCGSSCLCPVWLKKTMKAFSFLNVFAMFLQLQEEIMTVSRLEQAMDQLRKRGITLCMEDI 300

Query: 301  KHLSLLCPKAVHFASGSLEDTGDDTLIIIISLTEQNGRWKDD----------NTACKSFK 360
            ++LSLLCPKAVHFASG  EDT  D LIIII LTE+NGRWKDD           T+CKSF 
Sbjct: 301  QNLSLLCPKAVHFASGRSEDTCVDRLIIIIYLTEKNGRWKDDIDNTVTSHRCCTSCKSFT 360

Query: 361  APTDVTLLERRERSFKIFLLEAIKSHMLRHGSRSEICVPFSLEDLITSKESAVDGNEAKR 420
            APT+VT L+RRERSFK +LLEAIK HML+HGSR EICVPFSLE LIT  E+ VDG+EAKR
Sbjct: 361  APTNVTPLKRRERSFKFYLLEAIKCHMLKHGSRREICVPFSLEALITPNETDVDGSEAKR 420

Query: 421  AKKSETASLSSNSDRMQCHDTSKLLPENMVEHLVNGVGSEGQIVHIEDIAARKANYVEIP 480
             KK +TAS SS SDR QCHDTSKLLPE MVEHL  GVGSEGQIVH+EDI ARKANYVEIP
Sbjct: 421  GKKCDTAS-SSQSDRRQCHDTSKLLPEKMVEHLEKGVGSEGQIVHVEDIGARKANYVEIP 480

Query: 481  EELSNNVVSALKCIGVAKLYSHQAQSIEASLAGKNVAVATMTSSGKSLCYNLPVLESMSQ 540
            +ELSNNVVSALKCIGVA LYSHQAQSIEASLAGK+VAVATMTSSGKSLCYNLPVLE+MSQ
Sbjct: 481  KELSNNVVSALKCIGVANLYSHQAQSIEASLAGKHVAVATMTSSGKSLCYNLPVLEAMSQ 540

Query: 541  NVSSCALYLFPTKALAQDQLRSLLVMMKGFNDNVDIGVYDGDTSQADRIFLRDNARLLIT 600
            NVSSCALYLFPTKALAQDQLRSLL+MMKGFN N++IGVYDGDTSQADRI LRDNARLLIT
Sbjct: 541  NVSSCALYLFPTKALAQDQLRSLLIMMKGFNHNLNIGVYDGDTSQADRILLRDNARLLIT 600

Query: 601  NPDMLHLSILPHHRQFSRILSNLRFIVIDEAHTYKGAFGCHTALILRRLCRLCCHVYGSD 660
            NPDMLH+SILPHHRQFSRILSNLRFIVIDEAHTYKGAFGCHTALILRRL RLC HVYGSD
Sbjct: 601  NPDMLHVSILPHHRQFSRILSNLRFIVIDEAHTYKGAFGCHTALILRRLRRLCSHVYGSD 660

Query: 661  PSFIFCTATSANPRQHCMELGNLSSLELIEIDGSPSARKLFILWNPIMVLSLAQ--LWTL 720
            PSFIFCTATSANPR+HCMELGNLSSLELIE DGSPSARKLFILWNPIM L   Q  + + 
Sbjct: 661  PSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIMALKNYQRGIDSP 720

Query: 721  QGSLQKWF------SMDFAALLFVKLVNFVN--LFYATRR---------RELLKESAPHL 780
            Q + +          MD A L    +++ +    F  TR+         RE+LKESAPHL
Sbjct: 721  QSTKKNMTVRNPSPIMDIARLFAEMVLHGLRCIAFCKTRKLCELVLCYTREILKESAPHL 780

Query: 781  VQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFP----- 840
            VQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFP     
Sbjct: 781  VQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIAS 840

Query: 841  -----GRAGRREKTSLSVYVAFEGPLDQYFMKHPEKLFGSPIECCHIDAENQQVLEQHLL 900
                 GRAGRREKTSLSVYVAFEGPLDQYF+KHPEKLFGSPIECCHIDA+NQQVLEQHLL
Sbjct: 841  LWQQAGRAGRREKTSLSVYVAFEGPLDQYFVKHPEKLFGSPIECCHIDAKNQQVLEQHLL 900

Query: 901  CAAYEHPVNVVYDQKFFGPGLNTALISLKSRGDLIPEPSCGSSKSIWNYIGQEKMPSRLV 960
            CAAYEHPV V YDQ FFGPGLNTAL+SLK+RGDLI E SCGSSKSIWNYIGQEKMPSR V
Sbjct: 901  CAAYEHPVCVAYDQNFFGPGLNTALMSLKNRGDLIAESSCGSSKSIWNYIGQEKMPSRSV 960

Query: 961  SIRAIETERYKVVDQRQNEVLEEIEESTAFFQVYEGAVYLHQGRTYLVKSLNLSTMLAFC 1020
            SIRAIE ERYKVVDQRQNE+LEEIEES AFFQVYEGAVY+HQGRTYLVKSLNLSTMLAFC
Sbjct: 961  SIRAIEAERYKVVDQRQNEILEEIEESKAFFQVYEGAVYMHQGRTYLVKSLNLSTMLAFC 1020

Query: 1021 EEADLKYYTKTRDFTDVHVIGA-------------------------------------- 1044
            EEADLKYYTKTRD+TD+HVI                                        
Sbjct: 1021 EEADLKYYTKTRDYTDIHVIDGNLAYPRRAPNIPPSKTTAQANDCRVTTTWFGFYQIQKG 1080

BLAST of Spg022152 vs. NCBI nr
Match: XP_038878315.1 (uncharacterized ATP-dependent helicase YprA isoform X8 [Benincasa hispida])

HSP 1 Score: 1684.8 bits (4362), Expect = 0.0e+00
Identity = 891/1143 (77.95%), Postives = 950/1143 (83.11%), Query Frame = 0

Query: 1    MEESEREIGIKTLTGESLTVSISGDRTIEDLKLLLRRNFPSATISPNFHLFFKGSKLKPQ 60
            MEES REI IKTLTGESLTVSISG+RTI+DLKLLLRRNFPSATISPNFHLFFKG+KLKPQ
Sbjct: 1    MEESVREIEIKTLTGESLTVSISGNRTIDDLKLLLRRNFPSATISPNFHLFFKGTKLKPQ 60

Query: 61   SQISACRIDNGEFLVLIPFNKKESSKSLLRDQYEQGSSVSGGSSISQFADSAWSDMVQDL 120
            SQISACRID+GEFLVLIPFNKKESSKS LRDQYEQGSSVS GSSISQFADSAWSDMVQDL
Sbjct: 61   SQISACRIDHGEFLVLIPFNKKESSKSRLRDQYEQGSSVSCGSSISQFADSAWSDMVQDL 120

Query: 121  SYLHDCSTRGREDNGSERERGNSEVGGVDAELVATRSTSSSSSKAKGRKGFVYNGLKGNL 180
            SYLH CS   RE+N  E ERG+SEVGGVDAELVAT STSS SSK+KG+KGF Y+ LKGNL
Sbjct: 121  SYLHGCSDERREENRPESERGSSEVGGVDAELVATCSTSSFSSKSKGKKGFAYDDLKGNL 180

Query: 181  DDVLRNLLSSPAEGGLSERTCENFVKFLESVDCLSDPRNGKCMLANQVSSRSGNKRAPNR 240
            DDVLR   SSP+EG L+E+TCE+F+K+LESVDCLSDPRNG CMLA Q +SRSGNKRAPN 
Sbjct: 181  DDVLRKFSSSPSEGFLNEQTCESFIKYLESVDCLSDPRNGNCMLAKQANSRSGNKRAPNS 240

Query: 241  TCGSSCLCPGWLKKIMKAFAFLNVFSMFLQLQEENMTVSRLEQAMDLLQKHGITLCMEDM 300
            TCGSSCLCP WLKK MKAF+FLNVF+MFLQLQEE MTVSRLEQAMD L+K GITLCMED+
Sbjct: 241  TCGSSCLCPVWLKKTMKAFSFLNVFAMFLQLQEEIMTVSRLEQAMDQLRKRGITLCMEDI 300

Query: 301  KHLSLLCPKAVHFASGSLEDTGDDTLIIIISLTEQNGRWKDD----------NTACKSFK 360
            ++LSLLCPKAVHFASG  EDT  D LIIII LTE+NGRWKDD           T+CKSF 
Sbjct: 301  QNLSLLCPKAVHFASGRSEDTCVDRLIIIIYLTEKNGRWKDDIDNTVTSHRCCTSCKSFT 360

Query: 361  APTDVTLLERRERSFKIFLLEAIKSHMLRHGSRSEICVPFSLEDLITSKESAVDGNEAKR 420
            APT+VT L+RRERSFK +LLEAIK HML+HGSR EICVPFSLE LIT  E+ VDG+EAKR
Sbjct: 361  APTNVTPLKRRERSFKFYLLEAIKCHMLKHGSRREICVPFSLEALITPNETDVDGSEAKR 420

Query: 421  AKKSETASLSSNSDRMQCHDTSKLLPENMVEHLVNGVGSEGQIVHIEDIAARKANYVEIP 480
             KK +TAS SS SDR QCHDTSKLLPE MVEHL  GVGSEGQIVH+EDI ARKANYVEIP
Sbjct: 421  GKKCDTAS-SSQSDRRQCHDTSKLLPEKMVEHLEKGVGSEGQIVHVEDIGARKANYVEIP 480

Query: 481  EELSNNVVSALKCIGVAKLYSHQAQSIEASLAGKNVAVATMTSSGKSLCYNLPVLESMSQ 540
            +ELSNNVVSALKCIGVA LYSHQAQSIEASLAGK+VAVATMTSSGKSLCYNLPVLE+MSQ
Sbjct: 481  KELSNNVVSALKCIGVANLYSHQAQSIEASLAGKHVAVATMTSSGKSLCYNLPVLEAMSQ 540

Query: 541  NVSSCALYLFPTKALAQDQLRSLLVMMKGFNDNVDIGVYDGDTSQADRIFLRDNARLLIT 600
            NVSSCALYLFPTKALAQDQLRSLL+MMKGFN N++IGVYDGDTSQADRI LRDNARLLIT
Sbjct: 541  NVSSCALYLFPTKALAQDQLRSLLIMMKGFNHNLNIGVYDGDTSQADRILLRDNARLLIT 600

Query: 601  NPDMLHLSILPHHRQFSRILSNLRFIVIDEAHTYKGAFGCHTALILRRLCRLCCHVYGSD 660
            NPDMLH+SILPHHRQFSRILSNLRFIVIDEAHTYKGAFGCHTALILRRL RLC HVYGSD
Sbjct: 601  NPDMLHVSILPHHRQFSRILSNLRFIVIDEAHTYKGAFGCHTALILRRLRRLCSHVYGSD 660

Query: 661  PSFIFCTATSANPRQHCMELGNLSSLELIEIDGSPSARKLFILWNPIMVLSLAQ--LWTL 720
            PSFIFCTATSANPR+HCMELGNLSSLELIE DGSPSARKLFILWNPIM L   Q  + + 
Sbjct: 661  PSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIMALKNYQRGIDSP 720

Query: 721  QGSLQKWF------SMDFAALLFVKLVNFVN--LFYATRR---------RELLKESAPHL 780
            Q + +          MD A L    +++ +    F  TR+         RE+LKESAPHL
Sbjct: 721  QSTKKNMTVRNPSPIMDIARLFAEMVLHGLRCIAFCKTRKLCELVLCYTREILKESAPHL 780

Query: 781  VQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFP----- 840
            VQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFP     
Sbjct: 781  VQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIAS 840

Query: 841  -----GRAGRREKTSLSVYVAFEGPLDQYFMKHPEKLFGSPIECCHIDAENQQVLEQHLL 900
                 GRAGRREKTSLSVYVAFEGPLDQYF+KHPEKLFGSPIECCHIDA+NQQVLEQHLL
Sbjct: 841  LWQQAGRAGRREKTSLSVYVAFEGPLDQYFVKHPEKLFGSPIECCHIDAKNQQVLEQHLL 900

Query: 901  CAAYEHPVNVVYDQKFFGPGLNTALISLKSRGDLIPEPSCGSSKSIWNYIGQEKMPSRLV 960
            CAAYEHPV V YDQ FFGPGLNTAL+SLK+RGDLI E SCGSSKSIWNYIGQEKMPSR V
Sbjct: 901  CAAYEHPVCVAYDQNFFGPGLNTALMSLKNRGDLIAESSCGSSKSIWNYIGQEKMPSRSV 960

Query: 961  SIRAIETERYKVVDQRQNEVLEEIEESTAFFQ-----VYEGAVYLHQGRTYLVKSLNLST 1020
            SIRAIE ERYKVVDQRQNE+LEEIEES AFFQ     VYEGAVY+HQGRTYLVKSLNLST
Sbjct: 961  SIRAIEAERYKVVDQRQNEILEEIEESKAFFQVTAASVYEGAVYMHQGRTYLVKSLNLST 1020

Query: 1021 MLAFCEEADLKYYTKTRDFTDVHVIGA--------------------------------- 1044
            MLAFCEEADLKYYTKTRD+TD+HVI                                   
Sbjct: 1021 MLAFCEEADLKYYTKTRDYTDIHVIDGNLAYPRRAPNIPPSKTTAQANDCRVTTTWFGFY 1080

BLAST of Spg022152 vs. NCBI nr
Match: XP_038878306.1 (uncharacterized ATP-dependent helicase YprA isoform X2 [Benincasa hispida] >XP_038878307.1 uncharacterized ATP-dependent helicase YprA isoform X2 [Benincasa hispida] >XP_038878308.1 uncharacterized ATP-dependent helicase YprA isoform X2 [Benincasa hispida] >XP_038878309.1 uncharacterized ATP-dependent helicase YprA isoform X3 [Benincasa hispida])

HSP 1 Score: 1684.8 bits (4362), Expect = 0.0e+00
Identity = 891/1143 (77.95%), Postives = 950/1143 (83.11%), Query Frame = 0

Query: 1    MEESEREIGIKTLTGESLTVSISGDRTIEDLKLLLRRNFPSATISPNFHLFFKGSKLKPQ 60
            MEES REI IKTLTGESLTVSISG+RTI+DLKLLLRRNFPSATISPNFHLFFKG+KLKPQ
Sbjct: 1    MEESVREIEIKTLTGESLTVSISGNRTIDDLKLLLRRNFPSATISPNFHLFFKGTKLKPQ 60

Query: 61   SQISACRIDNGEFLVLIPFNKKESSKSLLRDQYEQGSSVSGGSSISQFADSAWSDMVQDL 120
            SQISACRID+GEFLVLIPFNKKESSKS LRDQYEQGSSVS GSSISQFADSAWSDMVQDL
Sbjct: 61   SQISACRIDHGEFLVLIPFNKKESSKSRLRDQYEQGSSVSCGSSISQFADSAWSDMVQDL 120

Query: 121  SYLHDCSTRGREDNGSERERGNSEVGGVDAELVATRSTSSSSSKAKGRKGFVYNGLKGNL 180
            SYLH CS   RE+N  E ERG+SEVGGVDAELVAT STSS SSK+KG+KGF Y+ LKGNL
Sbjct: 121  SYLHGCSDERREENRPESERGSSEVGGVDAELVATCSTSSFSSKSKGKKGFAYDDLKGNL 180

Query: 181  DDVLRNLLSSPAEGGLSERTCENFVKFLESVDCLSDPRNGKCMLANQVSSRSGNKRAPNR 240
            DDVLR   SSP+EG L+E+TCE+F+K+LESVDCLSDPRNG CMLA Q +SRSGNKRAPN 
Sbjct: 181  DDVLRKFSSSPSEGFLNEQTCESFIKYLESVDCLSDPRNGNCMLAKQANSRSGNKRAPNS 240

Query: 241  TCGSSCLCPGWLKKIMKAFAFLNVFSMFLQLQEENMTVSRLEQAMDLLQKHGITLCMEDM 300
            TCGSSCLCP WLKK MKAF+FLNVF+MFLQLQEE MTVSRLEQAMD L+K GITLCMED+
Sbjct: 241  TCGSSCLCPVWLKKTMKAFSFLNVFAMFLQLQEEIMTVSRLEQAMDQLRKRGITLCMEDI 300

Query: 301  KHLSLLCPKAVHFASGSLEDTGDDTLIIIISLTEQNGRWKDD----------NTACKSFK 360
            ++LSLLCPKAVHFASG  EDT  D LIIII LTE+NGRWKDD           T+CKSF 
Sbjct: 301  QNLSLLCPKAVHFASGRSEDTCVDRLIIIIYLTEKNGRWKDDIDNTVTSHRCCTSCKSFT 360

Query: 361  APTDVTLLERRERSFKIFLLEAIKSHMLRHGSRSEICVPFSLEDLITSKESAVDGNEAKR 420
            APT+VT L+RRERSFK +LLEAIK HML+HGSR EICVPFSLE LIT  E+ VDG+EAKR
Sbjct: 361  APTNVTPLKRRERSFKFYLLEAIKCHMLKHGSRREICVPFSLEALITPNETDVDGSEAKR 420

Query: 421  AKKSETASLSSNSDRMQCHDTSKLLPENMVEHLVNGVGSEGQIVHIEDIAARKANYVEIP 480
             KK +TAS SS SDR QCHDTSKLLPE MVEHL  GVGSEGQIVH+EDI ARKANYVEIP
Sbjct: 421  GKKCDTAS-SSQSDRRQCHDTSKLLPEKMVEHLEKGVGSEGQIVHVEDIGARKANYVEIP 480

Query: 481  EELSNNVVSALKCIGVAKLYSHQAQSIEASLAGKNVAVATMTSSGKSLCYNLPVLESMSQ 540
            +ELSNNVVSALKCIGVA LYSHQAQSIEASLAGK+VAVATMTSSGKSLCYNLPVLE+MSQ
Sbjct: 481  KELSNNVVSALKCIGVANLYSHQAQSIEASLAGKHVAVATMTSSGKSLCYNLPVLEAMSQ 540

Query: 541  NVSSCALYLFPTKALAQDQLRSLLVMMKGFNDNVDIGVYDGDTSQADRIFLRDNARLLIT 600
            NVSSCALYLFPTKALAQDQLRSLL+MMKGFN N++IGVYDGDTSQADRI LRDNARLLIT
Sbjct: 541  NVSSCALYLFPTKALAQDQLRSLLIMMKGFNHNLNIGVYDGDTSQADRILLRDNARLLIT 600

Query: 601  NPDMLHLSILPHHRQFSRILSNLRFIVIDEAHTYKGAFGCHTALILRRLCRLCCHVYGSD 660
            NPDMLH+SILPHHRQFSRILSNLRFIVIDEAHTYKGAFGCHTALILRRL RLC HVYGSD
Sbjct: 601  NPDMLHVSILPHHRQFSRILSNLRFIVIDEAHTYKGAFGCHTALILRRLRRLCSHVYGSD 660

Query: 661  PSFIFCTATSANPRQHCMELGNLSSLELIEIDGSPSARKLFILWNPIMVLSLAQ--LWTL 720
            PSFIFCTATSANPR+HCMELGNLSSLELIE DGSPSARKLFILWNPIM L   Q  + + 
Sbjct: 661  PSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIMALKNYQRGIDSP 720

Query: 721  QGSLQKWF------SMDFAALLFVKLVNFVN--LFYATRR---------RELLKESAPHL 780
            Q + +          MD A L    +++ +    F  TR+         RE+LKESAPHL
Sbjct: 721  QSTKKNMTVRNPSPIMDIARLFAEMVLHGLRCIAFCKTRKLCELVLCYTREILKESAPHL 780

Query: 781  VQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFP----- 840
            VQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFP     
Sbjct: 781  VQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIAS 840

Query: 841  -----GRAGRREKTSLSVYVAFEGPLDQYFMKHPEKLFGSPIECCHIDAENQQVLEQHLL 900
                 GRAGRREKTSLSVYVAFEGPLDQYF+KHPEKLFGSPIECCHIDA+NQQVLEQHLL
Sbjct: 841  LWQQAGRAGRREKTSLSVYVAFEGPLDQYFVKHPEKLFGSPIECCHIDAKNQQVLEQHLL 900

Query: 901  CAAYEHPVNVVYDQKFFGPGLNTALISLKSRGDLIPEPSCGSSKSIWNYIGQEKMPSRLV 960
            CAAYEHPV V YDQ FFGPGLNTAL+SLK+RGDLI E SCGSSKSIWNYIGQEKMPSR V
Sbjct: 901  CAAYEHPVCVAYDQNFFGPGLNTALMSLKNRGDLIAESSCGSSKSIWNYIGQEKMPSRSV 960

Query: 961  SIRAIETERYKVVDQRQNEVLEEIEESTAFFQ-----VYEGAVYLHQGRTYLVKSLNLST 1020
            SIRAIE ERYKVVDQRQNE+LEEIEES AFFQ     VYEGAVY+HQGRTYLVKSLNLST
Sbjct: 961  SIRAIEAERYKVVDQRQNEILEEIEESKAFFQVTAASVYEGAVYMHQGRTYLVKSLNLST 1020

Query: 1021 MLAFCEEADLKYYTKTRDFTDVHVIGA--------------------------------- 1044
            MLAFCEEADLKYYTKTRD+TD+HVI                                   
Sbjct: 1021 MLAFCEEADLKYYTKTRDYTDIHVIDGNLAYPRRAPNIPPSKTTAQANDCRVTTTWFGFY 1080

BLAST of Spg022152 vs. NCBI nr
Match: XP_038878301.1 (uncharacterized ATP-dependent helicase YprA isoform X1 [Benincasa hispida] >XP_038878304.1 uncharacterized ATP-dependent helicase YprA isoform X1 [Benincasa hispida] >XP_038878305.1 uncharacterized ATP-dependent helicase YprA isoform X1 [Benincasa hispida])

HSP 1 Score: 1684.8 bits (4362), Expect = 0.0e+00
Identity = 891/1143 (77.95%), Postives = 950/1143 (83.11%), Query Frame = 0

Query: 1    MEESEREIGIKTLTGESLTVSISGDRTIEDLKLLLRRNFPSATISPNFHLFFKGSKLKPQ 60
            MEES REI IKTLTGESLTVSISG+RTI+DLKLLLRRNFPSATISPNFHLFFKG+KLKPQ
Sbjct: 1    MEESVREIEIKTLTGESLTVSISGNRTIDDLKLLLRRNFPSATISPNFHLFFKGTKLKPQ 60

Query: 61   SQISACRIDNGEFLVLIPFNKKESSKSLLRDQYEQGSSVSGGSSISQFADSAWSDMVQDL 120
            SQISACRID+GEFLVLIPFNKKESSKS LRDQYEQGSSVS GSSISQFADSAWSDMVQDL
Sbjct: 61   SQISACRIDHGEFLVLIPFNKKESSKSRLRDQYEQGSSVSCGSSISQFADSAWSDMVQDL 120

Query: 121  SYLHDCSTRGREDNGSERERGNSEVGGVDAELVATRSTSSSSSKAKGRKGFVYNGLKGNL 180
            SYLH CS   RE+N  E ERG+SEVGGVDAELVAT STSS SSK+KG+KGF Y+ LKGNL
Sbjct: 121  SYLHGCSDERREENRPESERGSSEVGGVDAELVATCSTSSFSSKSKGKKGFAYDDLKGNL 180

Query: 181  DDVLRNLLSSPAEGGLSERTCENFVKFLESVDCLSDPRNGKCMLANQVSSRSGNKRAPNR 240
            DDVLR   SSP+EG L+E+TCE+F+K+LESVDCLSDPRNG CMLA Q +SRSGNKRAPN 
Sbjct: 181  DDVLRKFSSSPSEGFLNEQTCESFIKYLESVDCLSDPRNGNCMLAKQANSRSGNKRAPNS 240

Query: 241  TCGSSCLCPGWLKKIMKAFAFLNVFSMFLQLQEENMTVSRLEQAMDLLQKHGITLCMEDM 300
            TCGSSCLCP WLKK MKAF+FLNVF+MFLQLQEE MTVSRLEQAMD L+K GITLCMED+
Sbjct: 241  TCGSSCLCPVWLKKTMKAFSFLNVFAMFLQLQEEIMTVSRLEQAMDQLRKRGITLCMEDI 300

Query: 301  KHLSLLCPKAVHFASGSLEDTGDDTLIIIISLTEQNGRWKDD----------NTACKSFK 360
            ++LSLLCPKAVHFASG  EDT  D LIIII LTE+NGRWKDD           T+CKSF 
Sbjct: 301  QNLSLLCPKAVHFASGRSEDTCVDRLIIIIYLTEKNGRWKDDIDNTVTSHRCCTSCKSFT 360

Query: 361  APTDVTLLERRERSFKIFLLEAIKSHMLRHGSRSEICVPFSLEDLITSKESAVDGNEAKR 420
            APT+VT L+RRERSFK +LLEAIK HML+HGSR EICVPFSLE LIT  E+ VDG+EAKR
Sbjct: 361  APTNVTPLKRRERSFKFYLLEAIKCHMLKHGSRREICVPFSLEALITPNETDVDGSEAKR 420

Query: 421  AKKSETASLSSNSDRMQCHDTSKLLPENMVEHLVNGVGSEGQIVHIEDIAARKANYVEIP 480
             KK +TAS SS SDR QCHDTSKLLPE MVEHL  GVGSEGQIVH+EDI ARKANYVEIP
Sbjct: 421  GKKCDTAS-SSQSDRRQCHDTSKLLPEKMVEHLEKGVGSEGQIVHVEDIGARKANYVEIP 480

Query: 481  EELSNNVVSALKCIGVAKLYSHQAQSIEASLAGKNVAVATMTSSGKSLCYNLPVLESMSQ 540
            +ELSNNVVSALKCIGVA LYSHQAQSIEASLAGK+VAVATMTSSGKSLCYNLPVLE+MSQ
Sbjct: 481  KELSNNVVSALKCIGVANLYSHQAQSIEASLAGKHVAVATMTSSGKSLCYNLPVLEAMSQ 540

Query: 541  NVSSCALYLFPTKALAQDQLRSLLVMMKGFNDNVDIGVYDGDTSQADRIFLRDNARLLIT 600
            NVSSCALYLFPTKALAQDQLRSLL+MMKGFN N++IGVYDGDTSQADRI LRDNARLLIT
Sbjct: 541  NVSSCALYLFPTKALAQDQLRSLLIMMKGFNHNLNIGVYDGDTSQADRILLRDNARLLIT 600

Query: 601  NPDMLHLSILPHHRQFSRILSNLRFIVIDEAHTYKGAFGCHTALILRRLCRLCCHVYGSD 660
            NPDMLH+SILPHHRQFSRILSNLRFIVIDEAHTYKGAFGCHTALILRRL RLC HVYGSD
Sbjct: 601  NPDMLHVSILPHHRQFSRILSNLRFIVIDEAHTYKGAFGCHTALILRRLRRLCSHVYGSD 660

Query: 661  PSFIFCTATSANPRQHCMELGNLSSLELIEIDGSPSARKLFILWNPIMVLSLAQ--LWTL 720
            PSFIFCTATSANPR+HCMELGNLSSLELIE DGSPSARKLFILWNPIM L   Q  + + 
Sbjct: 661  PSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIMALKNYQRGIDSP 720

Query: 721  QGSLQKWF------SMDFAALLFVKLVNFVN--LFYATRR---------RELLKESAPHL 780
            Q + +          MD A L    +++ +    F  TR+         RE+LKESAPHL
Sbjct: 721  QSTKKNMTVRNPSPIMDIARLFAEMVLHGLRCIAFCKTRKLCELVLCYTREILKESAPHL 780

Query: 781  VQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFP----- 840
            VQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFP     
Sbjct: 781  VQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIAS 840

Query: 841  -----GRAGRREKTSLSVYVAFEGPLDQYFMKHPEKLFGSPIECCHIDAENQQVLEQHLL 900
                 GRAGRREKTSLSVYVAFEGPLDQYF+KHPEKLFGSPIECCHIDA+NQQVLEQHLL
Sbjct: 841  LWQQAGRAGRREKTSLSVYVAFEGPLDQYFVKHPEKLFGSPIECCHIDAKNQQVLEQHLL 900

Query: 901  CAAYEHPVNVVYDQKFFGPGLNTALISLKSRGDLIPEPSCGSSKSIWNYIGQEKMPSRLV 960
            CAAYEHPV V YDQ FFGPGLNTAL+SLK+RGDLI E SCGSSKSIWNYIGQEKMPSR V
Sbjct: 901  CAAYEHPVCVAYDQNFFGPGLNTALMSLKNRGDLIAESSCGSSKSIWNYIGQEKMPSRSV 960

Query: 961  SIRAIETERYKVVDQRQNEVLEEIEESTAFFQ-----VYEGAVYLHQGRTYLVKSLNLST 1020
            SIRAIE ERYKVVDQRQNE+LEEIEES AFFQ     VYEGAVY+HQGRTYLVKSLNLST
Sbjct: 961  SIRAIEAERYKVVDQRQNEILEEIEESKAFFQVTAASVYEGAVYMHQGRTYLVKSLNLST 1020

Query: 1021 MLAFCEEADLKYYTKTRDFTDVHVIGA--------------------------------- 1044
            MLAFCEEADLKYYTKTRD+TD+HVI                                   
Sbjct: 1021 MLAFCEEADLKYYTKTRDYTDIHVIDGNLAYPRRAPNIPPSKTTAQANDCRVTTTWFGFY 1080

BLAST of Spg022152 vs. ExPASy Swiss-Prot
Match: O13983 (ATP-dependent helicase hrq1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=hrq1 PE=1 SV=4)

HSP 1 Score: 367.5 bits (942), Expect = 5.1e-100
Identity = 227/681 (33.33%), Postives = 353/681 (51.84%), Query Frame = 0

Query: 440  VEHLVNGVGS----EGQIVH--IEDIAARKANYVEIPEELSNNVVSAL-KCIGVAKLYSH 499
            +E L+N + S    EGQIV   +    A +A Y  +   LS  +++AL     + K Y H
Sbjct: 256  IEELLNEIASESSYEGQIVQEALHTYPAVEAQYGALSRPLSQELINALYTSRNIEKTYKH 315

Query: 500  QAQSIEASLAGKNVAVATMTSSGKSLCYNLPVLESMSQNVSSCALYLFPTKALAQDQLRS 559
            QA +I     G +V V+T TSSGKSL Y +P+L+S+ ++  S A ++FPTK+LAQDQ +S
Sbjct: 316  QADAINHLWNGFHVIVSTSTSSGKSLIYQIPILQSLLEDNQSTAFFVFPTKSLAQDQKKS 375

Query: 560  LLVMMKGFN--DNVDIGVYDGDTSQADRIFLRDNARLLITNPDMLHLSILPHHRQFSRIL 619
            L+ ++       N+ +  +DGDT    R  +  +A ++ TNPDMLH +ILP+  ++    
Sbjct: 376  LIDILSYMPTLKNIRVDTFDGDTPLESRESIIRSANIIFTNPDMLHQTILPNANRWYYFF 435

Query: 620  SNLRFIVIDEAHTYKGAFGCHTALILRRLCRLCCHVYGSDPSFIFCTATSANPRQHCMEL 679
             NL+  V+DEAH Y G FG H A +LRR+ R+  +   S   F+ C+AT  +P QH  ++
Sbjct: 436  KNLKLFVLDEAHVYNGIFGVHVAFVLRRMRRIAEYFGNSQYRFVSCSATIEDPLQHMKKI 495

Query: 680  GNLSSLELIEIDGSPSARKLFILWNPIMV-------LSLAQLWTLQGSLQKWFSMDFAAL 739
              + +++LI    SPS  K F++WNP  V          + +      L K+       +
Sbjct: 496  FGVDNIKLINYTSSPSGSKKFVMWNPPYVDPKHPDDGKKSAISEASKLLIKFAEKRVRTI 555

Query: 740  LFVKLVNFVNLFYATRRRELLKESAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVA 799
            +F ++           R+EL  +    L+  + +YRAGYT ++RR+IES+ F G L G+ 
Sbjct: 556  VFCRVRKTCESLMRLVRQELKTKQKGDLLSKIQSYRAGYTVQERRKIESEMFNGKLYGII 615

Query: 800  ATNALELGIDVGHIDATLHLGFP----------GRAGRREKTSLSVYVAFEGPLDQYFMK 859
            ATNALELGID+G +DA + +GFP          GRAGRR K+SL+VY+    P+DQ+++K
Sbjct: 616  ATNALELGIDIGSLDAVITIGFPYSLSNLRQQFGRAGRRNKSSLAVYIVETFPVDQFYLK 675

Query: 860  HPEKLFGSPIECCHIDAENQQVLEQHLLCAAYEHPVNVVYDQKFFGPGLNTALISLKSRG 919
            HP  +   P     +D  N+ +L  HL CAAYE P+N+  D+KFFG   N      ++  
Sbjct: 676  HPILIHTQPNAELTLDLTNEVLLASHLQCAAYELPINIRSDEKFFG---NQIQDICEANL 735

Query: 920  DLIPEPSCGSSKSIWNYIGQEKMPSRLVSIRAIETERYKVVD--QRQNEVLEEIEESTAF 979
            +++ E      K +         P+  V IR++  + + +VD    +N +LE +E     
Sbjct: 736  EMVEESYRPHPKYL-------PFPASQVRIRSVSEDMFTLVDVTNDKNVILELLEPFRVA 795

Query: 980  FQVYEGAVYLHQGRTYLVKSLNLSTMLAFCEEADLKYYTKTRDFTDV------------- 1039
               YEGAVY++QG+T++++ LN++  +    + D+++ T  RDFTDV             
Sbjct: 796  LTAYEGAVYVYQGKTFIIRLLNINKRIITAHQVDVEWSTLQRDFTDVDPVRSLMKKTMHG 855

Query: 1040 -------------HVIG-----------------------AKQAVWIPVPQSIKEEVKRK 1044
                         HV G                         +  WI VP  I E +  K
Sbjct: 856  STNIYFGAVKATLHVFGYFKVNKQKDILDVVDITDHPVEIDSRGFWIDVPWHIIEVLSLK 915

BLAST of Spg022152 vs. ExPASy Swiss-Prot
Match: Q05549 (ATP-dependent helicase HRQ1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HRQ1 PE=1 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 5.3e-97
Identity = 250/783 (31.93%), Postives = 380/783 (48.53%), Query Frame = 0

Query: 350  KAPTDVT-----LLERRERSFKI----FLLEAIKSHMLRHGSRSEICVPFSLEDLITSKE 409
            K PT  T     ++ +RE  FK     F+LE  K+++      + +      +  I  + 
Sbjct: 154  KIPTYTTEEMKKMISKREALFKSRLREFILEKEKANLDPFSELTNLA-----QKYIPRER 213

Query: 410  SAVDGNEAKRAKKSETASLSSNSDRMQCHDTSKLLPENMVEHLVNGVGSEGQIVHIEDIA 469
               D  EA    K E+  +S        +     +P+ M+E L +      QI H   I 
Sbjct: 214  DYEDPIEAMMKAKQESNEMS--IPNYSNNSVITTIPQ-MIEKLKSTEFYASQIKHCFTIP 273

Query: 470  ARKANYVEIPEELSNNVVSALKCIGVAKLYSHQAQSIEASLAGKNVAVATMTSSGKSLCY 529
            +R A Y  +  EL+  V   ++       YSHQA +I +   G+NV + T TSSGKSL Y
Sbjct: 274  SRTAKYKGLCFELAPEVYQGME---HENFYSHQADAINSLHQGENVIITTSTSSGKSLIY 333

Query: 530  NLPVLESMSQNVSSCALYLFPTKALAQDQLRSLLVMMKGFND--NVDIGVYDGDTSQADR 589
             L  ++ + ++  S  +Y+FPTKALAQDQ R+  V++    +  N  +  YDGDT   +R
Sbjct: 334  QLAAIDLLLKDPESTFMYIFPTKALAQDQKRAFKVILSKIPELKNAVVDTYDGDTEPEER 393

Query: 590  IFLRDNARLLITNPDMLHLSILPHHRQFSRILSNLRFIVIDEAHTYKGAFGCHTALILRR 649
             ++R NAR++ TNPDM+H SILP+H  +   L +L+ +V+DE H YKG FG H AL++RR
Sbjct: 394  AYIRKNARVIFTNPDMIHTSILPNHANWRHFLYHLKLVVVDELHIYKGLFGSHVALVMRR 453

Query: 650  LCRLC-CHVYGSDPSFIFCTATSANPRQHCMELGNLSSLELIEIDGSPSARKLFILWNPI 709
            L RLC C    S   FI C+AT  +P QH  ++  ++ + LI  DGSP+  K  ++WNP 
Sbjct: 454  LLRLCHCFYENSGLQFISCSATLKSPVQHMKDMFGINEVTLIHEDGSPTGAKHLVVWNP- 513

Query: 710  MVLSLAQLWTLQGSLQKWFSMDFAALLFVKLVNFVNLF---YATRRRELLKESAPH---- 769
                   +       ++ F  + A +L   ++N V      Y  R  ELL +   +    
Sbjct: 514  ------PILPQHERKRENFIRESAKILVQLILNNVRTIAFCYVRRVCELLMKEVRNIFIE 573

Query: 770  -----LVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGF 829
                 LV  V +YR GY+A DRR+IE + F GNL  V +TNALELGID+G +DA L  GF
Sbjct: 574  TGREDLVTEVMSYRGGYSASDRRKIEREMFHGNLKAVISTNALELGIDIGGLDAVLMCGF 633

Query: 830  P----------GRAGRREKTSLSVYVAFEGPLDQYFMKHPEKLFG----SPIECCHIDAE 889
            P          GRAGRR   SL++ VA + P+DQ+++ HPE L         +   +D  
Sbjct: 634  PLSMANFHQQSGRAGRRNNDSLTLVVASDSPVDQHYVAHPESLLEVNNFESYQDLVLDFN 693

Query: 890  NQQVLEQHLLCAAYEHPVNVVYDQKFFGPGLNTALISLKSRGDLIPEPSCGSSKSIWNYI 949
            N  +LE H+ CAA+E P+N   D+++F       +   +   +   +    S++ +    
Sbjct: 694  NILILEGHIQCAAFELPINFERDKQYFTESHLRKICVERLHHN--QDGYHASNRFL---- 753

Query: 950  GQEKMPSRLVSIRAIETERYKVVD--QRQNEVLEEIEESTAFFQVYEGAVYLHQGRTYLV 1009
                 PS+ VS+R  E +++ VVD    +N ++EEIE S   F +Y+G +++HQG  YLV
Sbjct: 754  ---PWPSKCVSLRGGEEDQFAVVDITNGRNIIIEEIEASRTSFTLYDGGIFIHQGYPYLV 813

Query: 1010 KSLNLSTMLAFCEEADLKYYTKTRDFTDVH------------------------------ 1043
            K  N     A  +  D+ + T  RDFTDV                               
Sbjct: 814  KEFNPDERYAKVQRVDVDWVTNQRDFTDVDPQEIELIRSLRNSDVPVYFGKIKTTIIVFG 873

BLAST of Spg022152 vs. ExPASy Swiss-Prot
Match: P50830 (Uncharacterized ATP-dependent helicase YprA OS=Bacillus subtilis (strain 168) OX=224308 GN=yprA PE=1 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 8.2e-90
Identity = 203/574 (35.37%), Postives = 320/574 (55.75%), Query Frame = 0

Query: 435 LPENMVEHLVNGVGSEGQIVHIEDIAARKANYVEIPEELSNNVVSALKCIGVAKLYSHQA 494
           + +  +  L++ +     +V+  +I  R+A    +PE +   + +AL   G+ +LY+HQ 
Sbjct: 1   MKKKSLTELISDLKGNENVVNWHEIEPREAKTRPMPESIDERIKAALSKRGIDELYTHQY 60

Query: 495 QSIEASLAGKNVAVATMTSSGKSLCYNLPVLESMSQNVSSCALYLFPTKALAQDQLRSLL 554
            + +    G+++   T T+SGK+LCYNLPVL+S++Q+ ++ ALYLFPTKALAQDQ   L 
Sbjct: 61  SAFQYVQKGESIVTVTPTASGKTLCYNLPVLQSIAQDETNRALYLFPTKALAQDQKSELN 120

Query: 555 VMMKGFNDNVDIGVYDGDTSQADRIFLRDNARLLITNPDMLHLSILPHHRQFSRILSNLR 614
            ++     ++    YDGDTS A R  +R    ++ITNPDMLH +ILPHH ++  +  NL+
Sbjct: 121 EIIDEMGIDIKSFTYDGDTSPAIRQKVRKAGHIVITNPDMLHSAILPHHTKWVSLFENLK 180

Query: 615 FIVIDEAHTYKGAFGCHTALILRRLCRLCCHVYGSDPSFIFCTATSANPRQHCMELGNLS 674
           +IVIDE HTY+G FG H A ++RRL R+ C  YGSDP FI  +AT ANP++   +L    
Sbjct: 181 YIVIDELHTYRGVFGSHVANVIRRLKRI-CRFYGSDPVFICTSATIANPKELGEQLTG-K 240

Query: 675 SLELIEIDGSPSARKLFILWNPIMV---LSLAQLWTLQ-GSLQKWFSMD-FAALLFVKLV 734
            + L++ +G+PS RK F+ +NP +V   L++ +  T +   L K F  +    ++F +  
Sbjct: 241 PMRLVDDNGAPSGRKHFVFYNPPIVNKPLNIRRSATAEVNELAKEFLKNKVQTIVFARSR 300

Query: 735 NFVNLFYATRRRELLKESAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALE 794
             V +   +  +EL+K+      +S+  YR GY  ++RR IE     G++ GV +TNALE
Sbjct: 301 VRVEII-LSHIQELVKKEIG--TKSIRGYRGGYLPKERREIERGLREGDILGVVSTNALE 360

Query: 795 LGIDVGHIDATLHLGFP----------GRAGRREKTSLSVYVAFEGPLDQYFMKHPEKLF 854
           LG+D+G +   +  G+P          GRAGRR   SL + VA   P+DQY ++HPE  F
Sbjct: 361 LGVDIGQLQVCVMTGYPGSVASAWQQAGRAGRRHGESLIIMVANSTPIDQYIVRHPEYFF 420

Query: 855 GSPIECCHIDAENQQVLEQHLLCAAYEHPVNVVYDQKFFGPGLNTALISLKSRGDLIPEP 914
               E   I+ EN  +L  HL CAAYE P     D++F    ++  L  L+    L    
Sbjct: 421 NRSPESARINPENLIILVDHLKCAAYELPFRA--DEEFGAMEVSDILEYLQEEAVL---- 480

Query: 915 SCGSSKSIWNYIGQEKMPSRLVSIRAIETERYKVVDQ---RQNEVLEEIEESTAFFQVYE 974
                +  W     E  P+  +S+R+   E   +VDQ       ++ E++  +A   +++
Sbjct: 481 HRNGERYHW---ASESFPASNISLRSASQENVVIVDQSDIANVRIIGEMDRFSAMTLLHD 540

Query: 975 GAVYLHQGRTYLVKSLNLSTMLAFCEEADLKYYT 991
            A+YLH+G  Y V+ L+     A+  + D++YYT
Sbjct: 541 EAIYLHEGVQYQVEKLDWDHKKAYVRKVDVEYYT 560

BLAST of Spg022152 vs. ExPASy Swiss-Prot
Match: Q58969 (Uncharacterized ATP-dependent helicase MJ1574 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ1574 PE=3 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 3.9e-39
Identity = 122/435 (28.05%), Postives = 203/435 (46.67%), Query Frame = 0

Query: 448 GSEGQIVHIEDIAARKANYVEIPEELSNNVVSALKCIGVAKLYSHQAQSIEASLAGKNVA 507
           G E +I+ I  I  RK  +     +  N  ++ L      KLY HQ ++++     K+V 
Sbjct: 7   GIEKEIIKIYKIPERKGRFSNF--KFKNKEINELIDALGFKLYLHQVKALKYLYNKKDVV 66

Query: 508 VATMTSSGKSLCYNLPVLESMSQNVSSCALYLFPTKALAQDQLRSLLVMMKGF----NDN 567
           V T T+SGKS  + L + ++   N     L ++PT+AL  +Q     +  + F    N  
Sbjct: 67  VTTSTASGKSEIFRLAIFDNFLSNPDDRYLLIYPTRALINNQYEKFSMENELFYKITNKR 126

Query: 568 VDIGVYDGDTS-QADRIFLRDNARLLITNPDMLHLSILPHHRQFSRILSNLRFIVIDEAH 627
           V   +  GD   +  R  L+D   +L T PDMLH  IL +H  +  +L NL+ +V+DE H
Sbjct: 127 VKAEILTGDVGLEKRREILKDKPNVLFTTPDMLHYQILKNHNNYLWLLKNLKLLVVDELH 186

Query: 628 TYKGAFGCHTALILRRLCRLCCHVYGSDPSFIFCTATSANPRQHCMELGNLSSLELIEID 687
            Y+G FG +   + +RL +L   +  ++   +  +AT  NP++    L N    E+++  
Sbjct: 187 VYRGVFGTNMVYVFKRLLKLLKRL-NNNLQILCLSATLKNPKEFVKLLFN-RDFEVVDKS 246

Query: 688 GSPSARKLFILWNPIMVLSLAQLWTLQGSLQKWFSMDFAALLFVKLVNFVNLFYATRRRE 747
            +PS+RK   +  P    +L     L+  ++     +   L+F         F   +  E
Sbjct: 247 YNPSSRKYLAILEP---KNLDNKQLLRRLIENLVDNNIKTLVF---------FDTRKETE 306

Query: 748 LLKESA--PHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDAT 807
            L        +   +  Y+       R  IE  F  G +  +  TNALELGID+G +DA 
Sbjct: 307 KLMRFLLNSKVFYKLSTYKGTLPKYVREEIEEKFKNGEILALLTTNALELGIDIGDLDAV 366

Query: 808 LHLGFP-----------GRAGRREKTSLSVYVAFEGPLDQYFMKHPEKLF----GSPIEC 861
           ++ G P           GRAGRR+K +L++ V  +  LD Y+ +H  +L+       IE 
Sbjct: 367 INYGIPPDGIFSLIQRFGRAGRRDKEALNIIVLRKDGLDYYYKEHLNELYERIRKGIIEY 425

BLAST of Spg022152 vs. ExPASy Swiss-Prot
Match: Q57742 (Uncharacterized ATP-dependent helicase MJ0294 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ0294 PE=3 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 1.4e-12
Identity = 122/523 (23.33%), Postives = 218/523 (41.68%), Query Frame = 0

Query: 493 QAQSIEASLAGKNVAVATMTSSGKSLC-----YNLPVLESMSQNVSS--CALYLFPTKAL 552
           Q  +I+    GKNV + + T SGK+L       N  +  SM   +      LY+ P +AL
Sbjct: 39  QRYAIKEIHEGKNVLICSPTGSGKTLSAFLAGINELIKLSMENKLEDRIYILYVSPLRAL 98

Query: 553 AQDQLRSLLVMMKGFN----------DNVDIGVYDGDTSQADR-IFLRDNARLLITNPDM 612
             D  R+L   +K             D + + V   DT+ + +   L+    +LIT P+ 
Sbjct: 99  NNDIERNLKEPLKEIYDVAKEIGIELDEIRVAVRTSDTTSSQKQRMLKKPPHILITTPES 158

Query: 613 LHLSILPHHRQFSRILSNLRFIVIDEAHTYKGAFGCHTALILRRLCRLCCHVYGSDPSFI 672
           L +++  +  +FS++LS ++++++DE H      G H +L L RL R+   +     + I
Sbjct: 159 LAIAL--NSPKFSQLLSGIKYVIVDEIHALTNKRGVHLSLSLERLNRIANFIRIGLSATI 218

Query: 673 FCTATSANPRQHCMELGNLSSLELIEIDGSPSARKLFI------LWNPIMVLSLAQLWTL 732
                 A        +GN     ++++          I      ++ P   +S  +L+ L
Sbjct: 219 HPLTEVAK-----FLVGNGRDCYIVDVSYKKEIEIKVISPVDDFIYTPSEEIS-KRLYNL 278

Query: 733 QGSLQKWFSMDFAALLFVKLVNFVNLFYATRRREL-LKESAPHLVQSVCAYRAGYTAEDR 792
              L+K        L+      F N   AT R    LK+     V+ V  + +  + E R
Sbjct: 279 ---LKKLIEEHKTTLI------FTNTRSATERVAFYLKQLG---VEKVETHHSSLSREHR 338

Query: 793 RRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFP----------GRAGRR-EKTS 852
             +E     G +    ++ +LELG+D+G ID  + LG P          GR+G R  + S
Sbjct: 339 LEVEEKLKKGEIRVCISSTSLELGVDIGSIDLVILLGSPKSVSRALQRIGRSGHRLHEKS 398

Query: 853 LSVYVAFEGP--LDQYFMKHPEKLFGSPIECCHIDAENQQVLEQHLLCAAYEHPVNVVYD 912
             + + F+    ++   + +  K+    I+  HI      VL QHL+  A E   +V   
Sbjct: 399 KGIIIPFDRDDLVENVVLAYDAKI--GKIDRIHIPKNCLDVLAQHLVGMALEKVWDVDEA 458

Query: 913 QKFFGPGLNTALISLKSRGDLIPEPSCG-SSKSIWN--YIGQEKMPSRLVSIRAIETERY 968
                       +S K   D++   + G   K+++   ++   K   R  S+RAI     
Sbjct: 459 YNLIKKAYPYKDLSKKDFLDVLNYLAGGIEEKNVYAKIWLKDNKFGKRGKSVRAIYYMNV 518

BLAST of Spg022152 vs. ExPASy TrEMBL
Match: A0A5D3BGJ3 (Putative ATP-dependent helicase YprA isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold346G00090 PE=4 SV=1)

HSP 1 Score: 1696.0 bits (4391), Expect = 0.0e+00
Identity = 878/1071 (81.98%), Postives = 938/1071 (87.58%), Query Frame = 0

Query: 1    MEESEREIGIKTLTGESLTVSISGDRTIEDLKLLLRRNFPSATISPNFHLFFKGSKLKPQ 60
            MEESEREI IKTLTGES+TVS+SG+ TI+DLKLLLRRNFPSATISPNFHLFFKG+KLKPQ
Sbjct: 1    MEESEREIRIKTLTGESMTVSVSGNSTIDDLKLLLRRNFPSATISPNFHLFFKGTKLKPQ 60

Query: 61   SQISACRIDNGEFLVLIPFNKKESSKSLLRDQYEQGSSVSGGSSISQFADSAWSDMVQDL 120
            SQISAC ID+GEFLVLIPFNKKESSKS LRDQYE   SVS G+SISQFADSAWSDMVQDL
Sbjct: 61   SQISACCIDHGEFLVLIPFNKKESSKSRLRDQYELEFSVSSGTSISQFADSAWSDMVQDL 120

Query: 121  SYLHDCSTRGREDNGSERERGNSEVGGVDAELVATRSTSSSSSKAKGRKGFVYNGLKGNL 180
            SYLH CS  GRE+  SE ER +SEVG VDAEL A  STSS SSKAKG+ GF  +GL G+L
Sbjct: 121  SYLHGCSVEGREETVSESERASSEVGEVDAELEACCSTSSLSSKAKGKLGFGNDGLNGSL 180

Query: 181  DDVLRNLLSSPAEGGLSERTCENFVKFLESVDCLSDPRNGKCMLANQVSSRSGNKRAPNR 240
            DDVLRN   SP EG L+E+TCE+F+KFLESVDCL+DPRNG CMLA Q +SRSGNKRAPN 
Sbjct: 181  DDVLRNFSLSPTEGFLNEQTCESFIKFLESVDCLTDPRNGNCMLAKQANSRSGNKRAPNS 240

Query: 241  TCGSSCLCPGWLKKIMKAFAFLNVFSMFLQLQEENMTVSRLEQAMDLLQKHGITLCMEDM 300
            T GSSCLCP WLKKIMKAF+FLNVFSMFLQLQEE MTVSRLEQAMD LQK  I  CMED+
Sbjct: 241  TRGSSCLCPTWLKKIMKAFSFLNVFSMFLQLQEEIMTVSRLEQAMDQLQKRRIMFCMEDI 300

Query: 301  KHLSLLCPKAVHFASGSLEDTGDDTLIIIISLTEQNGRWKDDNTACKSFKAPTDVTLLER 360
             +LS LCPKAVHFASG +EDT    LIIII LTE+NGR K D     +      + LL R
Sbjct: 301  HNLSRLCPKAVHFASGIMEDTRVHKLIIIIYLTEKNGRPKKD---IDNTDGNATIYLLLR 360

Query: 361  RERSFKIFL-----------LEAIKSHMLRHGSRSEICVPFSLEDLITSKESAVDGNEAK 420
                   FL           L  IK  +L+HGS  E+CVPFSLE LITS E+ VDG+E K
Sbjct: 361  IPSYAPAFLNFNIQLGIVVSLLEIKMGLLKHGSGREMCVPFSLEALITSNETDVDGSETK 420

Query: 421  RAKKSETASLSSNSDRMQCHDTSKLLPENMVEHLVNGVGSEGQIVHIEDIAARKANYVEI 480
            RAKKS+TAS SS S+R++CHDTSKLLPENMVEHL   VG EGQIVH+ DI ARKANYVEI
Sbjct: 421  RAKKSDTASSSSQSERIRCHDTSKLLPENMVEHLKKSVGPEGQIVHVNDILARKANYVEI 480

Query: 481  PEELSNNVVSALKCIGVAKLYSHQAQSIEASLAGKNVAVATMTSSGKSLCYNLPVLESMS 540
            P+ELSN+VVSALKCIGVAKLYSHQA+SIEASL+GK+VAVATMTSSGKSLCYNLPVLE+MS
Sbjct: 481  PKELSNSVVSALKCIGVAKLYSHQARSIEASLSGKHVAVATMTSSGKSLCYNLPVLEAMS 540

Query: 541  QNVSSCALYLFPTKALAQDQLRSLLVMMKGFNDNVDIGVYDGDTSQADRIFLRDNARLLI 600
            Q+VSSCALYLFPTKALAQDQLRSLLVMMKGF  N++IGVYDGDTSQADRI LRDNARLLI
Sbjct: 541  QDVSSCALYLFPTKALAQDQLRSLLVMMKGFIHNLNIGVYDGDTSQADRILLRDNARLLI 600

Query: 601  TNPDMLHLSILPHHRQFSRILSNLRFIVIDEAHTYKGAFGCHTALILRRLCRLCCHVYGS 660
            TNPDMLH+SILPHHRQFSRILSNLRFIVIDEAHTYKGAFGCHTALILRRL R+C HVYGS
Sbjct: 601  TNPDMLHVSILPHHRQFSRILSNLRFIVIDEAHTYKGAFGCHTALILRRLRRICSHVYGS 660

Query: 661  DPSFIFCTATSANPRQHCMELGNLSSLELIEIDGSPSARKLFILWNPIMVLSLAQLWTLQ 720
            DPSFIFCTATSANPR+HCMELGNLSSLELI+ DGSPSARKLF+LWNP+MVL +AQLWTLQ
Sbjct: 661  DPSFIFCTATSANPREHCMELGNLSSLELIDNDGSPSARKLFLLWNPVMVLFVAQLWTLQ 720

Query: 721  GSLQKWFSMDFAALLFVKLVNFVNLFYATRRRELLKESAPHLVQSVCAYRAGYTAEDRRR 780
            GSL KWFSMDFAALLFVKL NFV+LFYATRRRE+LKESAPHLVQSVCAYRAGYTAEDRRR
Sbjct: 721  GSLPKWFSMDFAALLFVKLANFVSLFYATRRREILKESAPHLVQSVCAYRAGYTAEDRRR 780

Query: 781  IESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFP----------GRAGRREKTSLSV 840
            IESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFP          GRAGRREKTSLSV
Sbjct: 781  IESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQAGRAGRREKTSLSV 840

Query: 841  YVAFEGPLDQYFMKHPEKLFGSPIECCHIDAENQQVLEQHLLCAAYEHPVNVVYDQKFFG 900
            YVAFEGPLDQYFMKHPEKLFGSPIECCHIDAENQQVLEQHLLCAAYEHPV V YDQ FFG
Sbjct: 841  YVAFEGPLDQYFMKHPEKLFGSPIECCHIDAENQQVLEQHLLCAAYEHPVCVAYDQSFFG 900

Query: 901  PGLNTALISLKSRGDLIPEPSCGSSKSIWNYIGQEKMPSRLVSIRAIETERYKVVDQRQN 960
            PGLN AL+SLK+RGDLIPEPSCGSSKSIWNYIGQEKMPSR VSIRAIE ERYKVVDQR+N
Sbjct: 901  PGLNNALMSLKNRGDLIPEPSCGSSKSIWNYIGQEKMPSRSVSIRAIEAERYKVVDQRRN 960

Query: 961  EVLEEIEESTAFF-------QVYEGAVYLHQGRTYLVKSLNLSTMLAFCEEADLKYYTKT 1020
            EVLEEIEES AFF       QVYEGAVY+HQGRTYLVKSLNLSTMLAFCEEADLKYYTKT
Sbjct: 961  EVLEEIEESKAFFQVFGYVVQVYEGAVYMHQGRTYLVKSLNLSTMLAFCEEADLKYYTKT 1020

Query: 1021 RDFTDVHVIGAKQAVWIPVPQSIKEEVKRKNFDFRAGLHAASHALLNVVPL 1044
            RD+TD+HVIGA+QAVWIPVPQSIKEEVKRKNFDFRAGLHAASHALLNVVPL
Sbjct: 1021 RDYTDIHVIGAEQAVWIPVPQSIKEEVKRKNFDFRAGLHAASHALLNVVPL 1068

BLAST of Spg022152 vs. ExPASy TrEMBL
Match: A0A6J1L0Y0 (uncharacterized protein LOC111500077 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111500077 PE=4 SV=1)

HSP 1 Score: 1666.7 bits (4315), Expect = 0.0e+00
Identity = 879/1131 (77.72%), Postives = 939/1131 (83.02%), Query Frame = 0

Query: 1    MEESEREIGIKTLTGESLTVSISGDRTIEDLKLLLRRNFPSATISPNFHLFFKGSKLKPQ 60
            MEESE EI IKTLTGESLT+SISGDRTIEDLKLLLR+NFPSAT+SPNFHLF KG+KLKPQ
Sbjct: 1    MEESESEIRIKTLTGESLTISISGDRTIEDLKLLLRKNFPSATVSPNFHLFSKGTKLKPQ 60

Query: 61   SQISACRIDNGEFLVLIPFNKKESSKSLLRDQYEQGSSVSGGSSISQFADSAWSDMVQDL 120
            S+ISACRID GEFLVLIPF KKESSK  LRDQYEQGSSVSGGSSIS FADSAWSDMVQDL
Sbjct: 61   SEISACRIDKGEFLVLIPFTKKESSKPQLRDQYEQGSSVSGGSSISLFADSAWSDMVQDL 120

Query: 121  SYLHDCSTRGREDNGSERERGNSEVGGVDAELVATRSTSSSSSKAKGRKGFVYNGLKGNL 180
            SYLHDCS +GRE NG E ERGN E GGVD+EL AT +T  SS KA+G+KGF+ N   G L
Sbjct: 121  SYLHDCSVQGRERNGLESERGNFETGGVDSELAATCTTGPSSLKARGKKGFICNDSNGIL 180

Query: 181  DDVLRNLLSSPAEGGLSERTCENFVKFLESVDCLSDPRNGKCMLANQVSSRSGNKRAPNR 240
            DD+LRNLLSSP  G LSE  CEN +KFLESVDCLSDPRN KC+LA Q +SRSG+++APNR
Sbjct: 181  DDILRNLLSSPTVGLLSEYNCENLIKFLESVDCLSDPRNEKCLLAKQANSRSGSRKAPNR 240

Query: 241  TCGSSCLCPGWLKKIMKAFAFLNVFSMFLQLQEENMTVSRLEQAMDLLQKHGITLCMEDM 300
            T  SSCLCP WLKKIMK FAFLNV SMF QL+EE +T SRLEQAMDLLQKHGITL MEDM
Sbjct: 241  TRCSSCLCPVWLKKIMKTFAFLNVLSMFAQLREEIITASRLEQAMDLLQKHGITLRMEDM 300

Query: 301  KHLSLLCPKAVHFASGSLEDTGDDTLIIIISLTEQNGRWKDDNTACK-SFKAPTDVTLLE 360
            KHLSLLCPK VHFASG+LED+ DD +II+  LT QN RW  DNTA K S KAPTD+T ++
Sbjct: 301  KHLSLLCPKVVHFASGTLEDSYDDKIIIVNYLTAQNSRWTADNTAYKLSSKAPTDITPMK 360

Query: 361  RRERSFKIFLLEAIKSHMLRHGSRSEICVPFSLEDLITSKESAVDGNEAKRAKKSETASL 420
            RRE+SFK +L EAIK HMLRHGSRSEICV FSLEDLITSK SAVDGNEAKRAKKS+ AS 
Sbjct: 361  RREKSFKFYLWEAIKGHMLRHGSRSEICVSFSLEDLITSKASAVDGNEAKRAKKSDMASS 420

Query: 421  SSNSDRMQCHDTSKLLPENMVEHLVNGVGSEGQIVHIEDIAARKANYVEIPEELSNNVVS 480
            SS SDR+QCHDTSKLLPENMVEHL  G+GSEGQIVH+EDIAARKANYVEIPEELSNNV+S
Sbjct: 421  SSQSDRVQCHDTSKLLPENMVEHLEKGIGSEGQIVHVEDIAARKANYVEIPEELSNNVIS 480

Query: 481  ALKCIGVAKLYSHQAQSIEASLAGKNVAVATMTSSGKSLCYNLPVLESMSQNVSSCALYL 540
            ALKCIGV KLYSHQ +SIEASLAG +VAVATMTSSGKSLCYNLPVLESMSQ+VSSCALYL
Sbjct: 481  ALKCIGVEKLYSHQTRSIEASLAGNHVAVATMTSSGKSLCYNLPVLESMSQDVSSCALYL 540

Query: 541  FPTKALAQDQLRSLLVMMKGFNDNVDIGVYDGDTSQADRIFLRDNARLLITNPDMLHLSI 600
            FPTKALAQDQLRSLLVMMKGFN +++IGVYDGDTSQ+DRI LRDNARLLITNPDMLHLSI
Sbjct: 541  FPTKALAQDQLRSLLVMMKGFNADLNIGVYDGDTSQSDRILLRDNARLLITNPDMLHLSI 600

Query: 601  LPHHRQFSRILSNLRFIVIDEAHTYKGAFGCHTALILRRLCRLCCHVYGSDPSFIFCTAT 660
            LP HRQFSRILSNLRFIVIDEAHTYKGAFGCHTALI+RRL RLC HVYGSDPSFIFCTAT
Sbjct: 601  LPQHRQFSRILSNLRFIVIDEAHTYKGAFGCHTALIIRRLRRLCSHVYGSDPSFIFCTAT 660

Query: 661  SANPRQHCMELGNLSSLELIEIDGSPSARKLFILWNPIMVLSLAQ--LWTLQGSLQKWFS 720
            SANPRQHCMELG+LSSLELIE DGSPSARKLFILWNPIM    +Q  + +LQ S +K  +
Sbjct: 661  SANPRQHCMELGSLSSLELIENDGSPSARKLFILWNPIMASKSSQRGIDSLQ-STEKNAN 720

Query: 721  -------MDFAALLFVKLVNF---VNLFYATRR---------RELLKESAPHLVQSVCAY 780
                   MD A  LF ++V        F  TR+         RE+LKE APHLVQSVCAY
Sbjct: 721  FRNPSPIMDIAR-LFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKERAPHLVQSVCAY 780

Query: 781  RAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFP----------GR 840
            RAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFP          GR
Sbjct: 781  RAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQAGR 840

Query: 841  AGRREKTSLSVYVAFEGPLDQYFMKHPEKLFGSPIECCHIDAENQQVLEQHLLCAAYEHP 900
            AGRREKTSLSVYVAFEGPLDQYFMKHPEKLFGSPI+CCHIDAENQQVLEQHLLCAAYEHP
Sbjct: 841  AGRREKTSLSVYVAFEGPLDQYFMKHPEKLFGSPIKCCHIDAENQQVLEQHLLCAAYEHP 900

Query: 901  VNVVYDQKFFGPGLNTALISLKSRGDLIPEPSCGSSKSIWNYIGQEKMPSRLVSIRAIET 960
            V +VYDQ  FGPGLNTAL+SLKSRGDLIP PSCGSSKSIW YIG+EKMPSR VSIRAIE 
Sbjct: 901  VCMVYDQNLFGPGLNTALMSLKSRGDLIPAPSCGSSKSIWTYIGKEKMPSRSVSIRAIEA 960

Query: 961  ERYKVVDQRQNEVLEEIEESTAFFQVYEGAVYLHQGRTYLVKSLNLSTMLAFCEEADLKY 1020
            ERYKVVDQRQNEVLEEIEESTAFFQVYEGAVY+HQGRTYLVKSLNLSTMLAFCEEADLKY
Sbjct: 961  ERYKVVDQRQNEVLEEIEESTAFFQVYEGAVYMHQGRTYLVKSLNLSTMLAFCEEADLKY 1020

Query: 1021 YTKTRDFTDVHVIGA--------------------------------------------- 1044
            YTKTRD+TD+HVIG                                              
Sbjct: 1021 YTKTRDYTDIHVIGGSLAYPRRAPNIPLLKTTAQANDCRVTTTWFGFYRIWKGSNQIFDT 1080

BLAST of Spg022152 vs. ExPASy TrEMBL
Match: A0A6J1BZJ1 (uncharacterized protein LOC111007140 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007140 PE=4 SV=1)

HSP 1 Score: 1662.9 bits (4305), Expect = 0.0e+00
Identity = 875/1131 (77.37%), Postives = 947/1131 (83.73%), Query Frame = 0

Query: 1    MEESEREIGIKTLTGESLTVSISGDRTIEDLKLLLRRNFPSATISPNFHLFFKGSKLKPQ 60
            MEESEREI I+TLTGESLTV ISG RTI+DLKLLLRRNFPSAT SPNFHLF KGSKLKPQ
Sbjct: 1    MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSKGSKLKPQ 60

Query: 61   SQISACRIDNGEFLVLIPFNKKESSKSLLRDQYEQGSSVSGGSSISQFADSAWSDMVQDL 120
            S I ACRID+GEFLVLIPFNKKESSKS LRDQYEQ SSVS  SSISQFADSAWSDMVQDL
Sbjct: 61   SLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSISQFADSAWSDMVQDL 120

Query: 121  SYLHDCSTRGREDNGSERERGNSEVGGVDAELVATRSTSSSSSKAKGRKGFVYNGLKGNL 180
            SYLHDCS +GR++N  ERERGNSE+G V+AELV T S+SSSSSKAKG+KGFV+N LKGN 
Sbjct: 121  SYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAKGKKGFVFNDLKGN- 180

Query: 181  DDVLRNLLSSPAEGGLSERTCENFVKFLESVDCLSDPRNGKCMLANQVSSRSGNKRAPNR 240
            DDVLRNLLSS  EG L+E TCENF+KFLESVDCLSDPRNG+CMLA Q +SR  NK+ P+R
Sbjct: 181  DDVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQANSRGANKKVPHR 240

Query: 241  TCGSSCLCPGWLKKIMKAFAFLNVFSMFLQLQEENMTVSRLEQAMDLLQKHGITLCMEDM 300
            T   SCLCP WLKKI KAF+FLNVFSM LQLQE+ MTVSRLEQAMDLLQ HGIT+C ED+
Sbjct: 241  TYSPSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDLLQNHGITICTEDI 300

Query: 301  KHLSLLCPKAVHFASGSLEDTGDDTLIIIISLTEQNGRWKDDNTACK-SFKAPTDVTLLE 360
            KHLSLLCPKAVHFASGSLED+ DDTL I+I L+EQNGRW D NT    +  AP DVTLL+
Sbjct: 301  KHLSLLCPKAVHFASGSLEDSCDDTLSIVIYLSEQNGRWNDGNTGKHVNRDAPKDVTLLK 360

Query: 361  RRERSFKIFLLEAIKSHMLRHGSRSEICVPFSLEDLITSKESAVDGNEAKRAKKSETASL 420
            RRERSFK +L EAIK HMLRHGSRSEICVPFSLEDLIT KES+V G E KR KKS+TAS 
Sbjct: 361  RRERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGGEEKRRKKSDTASS 420

Query: 421  SSNSDRMQCHDTSKLLPENMVEHLVNGVGSEGQIVHIEDIAARKANYVEIPEELSNNVVS 480
            SS SD++QCHDTSKLLPE MVEHL  GVGS+GQIVH+EDIAARKANYVEIPEELSNNV+S
Sbjct: 421  SSKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANYVEIPEELSNNVIS 480

Query: 481  ALKCIGVAKLYSHQAQSIEASLAGKNVAVATMTSSGKSLCYNLPVLESMSQNVSSCALYL 540
            ALKCIGV KLYSHQA+SIEASLAGK+VAVATMTSSGKSLCYNLPVLESMSQNV+SCALYL
Sbjct: 481  ALKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLESMSQNVTSCALYL 540

Query: 541  FPTKALAQDQLRSLLVMMKGFNDNVDIGVYDGDTSQADRIFLRDNARLLITNPDMLHLSI 600
            FPTKALAQDQLRSLLVMMKGF+DN++IGVYDGDTSQADR+ LRDNARLLITNPDMLH+SI
Sbjct: 541  FPTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNARLLITNPDMLHVSI 600

Query: 601  LPHHRQFSRILSNLRFIVIDEAHTYKGAFGCHTALILRRLCRLCCHVYGSDPSFIFCTAT 660
            LP+HRQFSRILSNLRF++IDEAHTYKGAFGCHTALILRRL R+C HVYGSDPSFIFCTAT
Sbjct: 601  LPYHRQFSRILSNLRFVLIDEAHTYKGAFGCHTALILRRLRRICSHVYGSDPSFIFCTAT 660

Query: 661  SANPRQHCMELGNLSSLELIEIDGSPSARKLFILWNPIMVLSLAQ--LWTLQGSLQKWFS 720
            SANPR+HCMELGNLSSLELIE DGSPSARKLFILWNPI  L   Q  + +LQ S +K  +
Sbjct: 661  SANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRDIDSLQ-STEKNVN 720

Query: 721  -------MDFAALLFVKLVNF---VNLFYATRR---------RELLKESAPHLVQSVCAY 780
                   MD A  LF ++V        F  TR+         RE+LKE APHLVQSVCAY
Sbjct: 721  FRNPSPIMDIAR-LFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKERAPHLVQSVCAY 780

Query: 781  RAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFP----------GR 840
            RAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFP          GR
Sbjct: 781  RAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQAGR 840

Query: 841  AGRREKTSLSVYVAFEGPLDQYFMKHPEKLFGSPIECCHIDAENQQVLEQHLLCAAYEHP 900
            AGRRE+TSLSVYVAF+GPLDQYFMK+PEKLFGSPIECCHIDA NQQVLEQHLLCAA+EHP
Sbjct: 841  AGRRERTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGNQQVLEQHLLCAAHEHP 900

Query: 901  VNVVYDQKFFGPGLNTALISLKSRGDLIPEPSCGSSKSIWNYIGQEKMPSRLVSIRAIET 960
            ++V+YDQ FFG GLNTAL SLK+RGDLIP+PSCGSSKSIWNYIGQEKMPSR VSIRAIET
Sbjct: 901  ISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIGQEKMPSRAVSIRAIET 960

Query: 961  ERYKVVDQRQNEVLEEIEESTAFFQVYEGAVYLHQGRTYLVKSLNLSTMLAFCEEADLKY 1020
            ERY+VVDQRQNEV+EEIEES AFFQVYEGAVY+HQGRTYLVKSLNLS+MLAFCEEADLKY
Sbjct: 961  ERYRVVDQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSLNLSSMLAFCEEADLKY 1020

Query: 1021 YTKTRDFTDVHVIGA--------------------------------------------- 1044
            YTKTRD+TD+HVIG                                              
Sbjct: 1021 YTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTWFGFYRIWKGSKQIFDT 1080

BLAST of Spg022152 vs. ExPASy TrEMBL
Match: A0A6J1C3R1 (uncharacterized protein LOC111007140 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111007140 PE=4 SV=1)

HSP 1 Score: 1662.9 bits (4305), Expect = 0.0e+00
Identity = 875/1131 (77.37%), Postives = 947/1131 (83.73%), Query Frame = 0

Query: 1    MEESEREIGIKTLTGESLTVSISGDRTIEDLKLLLRRNFPSATISPNFHLFFKGSKLKPQ 60
            MEESEREI I+TLTGESLTV ISG RTI+DLKLLLRRNFPSAT SPNFHLF KGSKLKPQ
Sbjct: 1    MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSKGSKLKPQ 60

Query: 61   SQISACRIDNGEFLVLIPFNKKESSKSLLRDQYEQGSSVSGGSSISQFADSAWSDMVQDL 120
            S I ACRID+GEFLVLIPFNKKESSKS LRDQYEQ SSVS  SSISQFADSAWSDMVQDL
Sbjct: 61   SLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSISQFADSAWSDMVQDL 120

Query: 121  SYLHDCSTRGREDNGSERERGNSEVGGVDAELVATRSTSSSSSKAKGRKGFVYNGLKGNL 180
            SYLHDCS +GR++N  ERERGNSE+G V+AELV T S+SSSSSKAKG+KGFV+N LKGN 
Sbjct: 121  SYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAKGKKGFVFNDLKGN- 180

Query: 181  DDVLRNLLSSPAEGGLSERTCENFVKFLESVDCLSDPRNGKCMLANQVSSRSGNKRAPNR 240
            DDVLRNLLSS  EG L+E TCENF+KFLESVDCLSDPRNG+CMLA Q +SR  NK+ P+R
Sbjct: 181  DDVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQANSRGANKKVPHR 240

Query: 241  TCGSSCLCPGWLKKIMKAFAFLNVFSMFLQLQEENMTVSRLEQAMDLLQKHGITLCMEDM 300
            T   SCLCP WLKKI KAF+FLNVFSM LQLQE+ MTVSRLEQAMDLLQ HGIT+C ED+
Sbjct: 241  TYSPSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDLLQNHGITICTEDI 300

Query: 301  KHLSLLCPKAVHFASGSLEDTGDDTLIIIISLTEQNGRWKDDNTACK-SFKAPTDVTLLE 360
            KHLSLLCPKAVHFASGSLED+ DDTL I+I L+EQNGRW D NT    +  AP DVTLL+
Sbjct: 301  KHLSLLCPKAVHFASGSLEDSCDDTLSIVIYLSEQNGRWNDGNTGKHVNRDAPKDVTLLK 360

Query: 361  RRERSFKIFLLEAIKSHMLRHGSRSEICVPFSLEDLITSKESAVDGNEAKRAKKSETASL 420
            RRERSFK +L EAIK HMLRHGSRSEICVPFSLEDLIT KES+V G E KR KKS+TAS 
Sbjct: 361  RRERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGGEEKRRKKSDTASS 420

Query: 421  SSNSDRMQCHDTSKLLPENMVEHLVNGVGSEGQIVHIEDIAARKANYVEIPEELSNNVVS 480
            SS SD++QCHDTSKLLPE MVEHL  GVGS+GQIVH+EDIAARKANYVEIPEELSNNV+S
Sbjct: 421  SSKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANYVEIPEELSNNVIS 480

Query: 481  ALKCIGVAKLYSHQAQSIEASLAGKNVAVATMTSSGKSLCYNLPVLESMSQNVSSCALYL 540
            ALKCIGV KLYSHQA+SIEASLAGK+VAVATMTSSGKSLCYNLPVLESMSQNV+SCALYL
Sbjct: 481  ALKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLESMSQNVTSCALYL 540

Query: 541  FPTKALAQDQLRSLLVMMKGFNDNVDIGVYDGDTSQADRIFLRDNARLLITNPDMLHLSI 600
            FPTKALAQDQLRSLLVMMKGF+DN++IGVYDGDTSQADR+ LRDNARLLITNPDMLH+SI
Sbjct: 541  FPTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNARLLITNPDMLHVSI 600

Query: 601  LPHHRQFSRILSNLRFIVIDEAHTYKGAFGCHTALILRRLCRLCCHVYGSDPSFIFCTAT 660
            LP+HRQFSRILSNLRF++IDEAHTYKGAFGCHTALILRRL R+C HVYGSDPSFIFCTAT
Sbjct: 601  LPYHRQFSRILSNLRFVLIDEAHTYKGAFGCHTALILRRLRRICSHVYGSDPSFIFCTAT 660

Query: 661  SANPRQHCMELGNLSSLELIEIDGSPSARKLFILWNPIMVLSLAQ--LWTLQGSLQKWFS 720
            SANPR+HCMELGNLSSLELIE DGSPSARKLFILWNPI  L   Q  + +LQ S +K  +
Sbjct: 661  SANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRDIDSLQ-STEKNVN 720

Query: 721  -------MDFAALLFVKLVNF---VNLFYATRR---------RELLKESAPHLVQSVCAY 780
                   MD A  LF ++V        F  TR+         RE+LKE APHLVQSVCAY
Sbjct: 721  FRNPSPIMDIAR-LFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKERAPHLVQSVCAY 780

Query: 781  RAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFP----------GR 840
            RAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFP          GR
Sbjct: 781  RAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQAGR 840

Query: 841  AGRREKTSLSVYVAFEGPLDQYFMKHPEKLFGSPIECCHIDAENQQVLEQHLLCAAYEHP 900
            AGRRE+TSLSVYVAF+GPLDQYFMK+PEKLFGSPIECCHIDA NQQVLEQHLLCAA+EHP
Sbjct: 841  AGRRERTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGNQQVLEQHLLCAAHEHP 900

Query: 901  VNVVYDQKFFGPGLNTALISLKSRGDLIPEPSCGSSKSIWNYIGQEKMPSRLVSIRAIET 960
            ++V+YDQ FFG GLNTAL SLK+RGDLIP+PSCGSSKSIWNYIGQEKMPSR VSIRAIET
Sbjct: 901  ISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIGQEKMPSRAVSIRAIET 960

Query: 961  ERYKVVDQRQNEVLEEIEESTAFFQVYEGAVYLHQGRTYLVKSLNLSTMLAFCEEADLKY 1020
            ERY+VVDQRQNEV+EEIEES AFFQVYEGAVY+HQGRTYLVKSLNLS+MLAFCEEADLKY
Sbjct: 961  ERYRVVDQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSLNLSSMLAFCEEADLKY 1020

Query: 1021 YTKTRDFTDVHVIGA--------------------------------------------- 1044
            YTKTRD+TD+HVIG                                              
Sbjct: 1021 YTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTWFGFYRIWKGSKQIFDT 1080

BLAST of Spg022152 vs. ExPASy TrEMBL
Match: A0A6J1F581 (uncharacterized protein LOC111440956 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111440956 PE=4 SV=1)

HSP 1 Score: 1662.1 bits (4303), Expect = 0.0e+00
Identity = 876/1128 (77.66%), Postives = 935/1128 (82.89%), Query Frame = 0

Query: 3    ESEREIGIKTLTGESLTVSISGDRTIEDLKLLLRRNFPSATISPNFHLFFKGSKLKPQSQ 62
            ESE EI IKTLTGESLT+SISG+RTIEDLKLLLR+NFPSAT+SPNFHLF KG+KLKPQS+
Sbjct: 5    ESESEIRIKTLTGESLTISISGNRTIEDLKLLLRKNFPSATVSPNFHLFSKGTKLKPQSE 64

Query: 63   ISACRIDNGEFLVLIPFNKKESSKSLLRDQYEQGSSVSGGSSISQFADSAWSDMVQDLSY 122
            ISACRID GEFLVLIPF KKESSK  LRDQYEQGSSVSGGSSISQFADSAWSDMVQDLSY
Sbjct: 65   ISACRIDKGEFLVLIPFTKKESSKPQLRDQYEQGSSVSGGSSISQFADSAWSDMVQDLSY 124

Query: 123  LHDCSTRGREDNGSERERGNSEVGGVDAELVATRSTSSSSSKAKGRKGFVYNGLKGNLDD 182
            LHDCS +GRE NG E ERGN E GGVDAEL AT ST SSS KAKG+KGFV N   G LDD
Sbjct: 125  LHDCSVQGREGNGLESERGNFEAGGVDAELAATCSTGSSSLKAKGKKGFVCNDSNGILDD 184

Query: 183  VLRNLLSSPAEGGLSERTCENFVKFLESVDCLSDPRNGKCMLANQVSSRSGNKRAPNRTC 242
            +LRNLLSSP    LSE  CEN +K LESVDCLSDPRN KC+LA Q +SRSG+++APN T 
Sbjct: 185  ILRNLLSSPTVEFLSEYNCENLIKLLESVDCLSDPRNEKCLLAKQANSRSGSRKAPNGTR 244

Query: 243  GSSCLCPGWLKKIMKAFAFLNVFSMFLQLQEENMTVSRLEQAMDLLQKHGITLCMEDMKH 302
             SSCLCP WLKKIMKAFAFLNV SMF QL+EE +T SRLEQA+DLLQKHGITL MEDMKH
Sbjct: 245  CSSCLCPVWLKKIMKAFAFLNVLSMFAQLREEIITASRLEQAIDLLQKHGITLRMEDMKH 304

Query: 303  LSLLCPKAVHFASGSLEDTGDDTLIIIISLTEQNGRWKDDNTACKSFKAPTDVTLLERRE 362
            LSLLCPK VHFASG+LED+ DD +II+I LT QN RW  DNTA K  K PTD+T L+RRE
Sbjct: 305  LSLLCPKVVHFASGTLEDSYDDKIIIVIYLTAQNSRWTADNTAYKLSKGPTDITPLKRRE 364

Query: 363  RSFKIFLLEAIKSHMLRHGSRSEICVPFSLEDLITSKESAVDGNEAKRAKKSETASLSSN 422
            +SFK +L +AIK HMLRHGSRSEICV FSLEDLITSK SAVDGNEAKRAKKS+ AS SS 
Sbjct: 365  KSFKFYLWDAIKGHMLRHGSRSEICVSFSLEDLITSKASAVDGNEAKRAKKSDMASSSSK 424

Query: 423  SDRMQCHDTSKLLPENMVEHLVNGVGSEGQIVHIEDIAARKANYVEIPEELSNNVVSALK 482
            SDR+QCHDTSKLLPENMVEHL  G+GSEGQIVH+EDIAARKANYVEIPEELSNNV+SALK
Sbjct: 425  SDRVQCHDTSKLLPENMVEHLEKGIGSEGQIVHVEDIAARKANYVEIPEELSNNVISALK 484

Query: 483  CIGVAKLYSHQAQSIEASLAGKNVAVATMTSSGKSLCYNLPVLESMSQNVSSCALYLFPT 542
            CIGV KLYSHQ +SIEASLAG +VAVATMTSSGKSLCYNLPVLESMSQ+VSSCALYLFPT
Sbjct: 485  CIGVEKLYSHQTRSIEASLAGNHVAVATMTSSGKSLCYNLPVLESMSQDVSSCALYLFPT 544

Query: 543  KALAQDQLRSLLVMMKGFNDNVDIGVYDGDTSQADRIFLRDNARLLITNPDMLHLSILPH 602
            KALAQDQLRSLLVMMKGFN +++IGVYDGDTSQ+DRI LRDNARLLITNPDMLHLSILP 
Sbjct: 545  KALAQDQLRSLLVMMKGFNADLNIGVYDGDTSQSDRILLRDNARLLITNPDMLHLSILPQ 604

Query: 603  HRQFSRILSNLRFIVIDEAHTYKGAFGCHTALILRRLCRLCCHVYGSDPSFIFCTATSAN 662
            HRQFSRILSNLRFIVIDEAHTYKGAFGCHTALI+RRL RLC HVYGSDPSFIFCTATSAN
Sbjct: 605  HRQFSRILSNLRFIVIDEAHTYKGAFGCHTALIIRRLRRLCSHVYGSDPSFIFCTATSAN 664

Query: 663  PRQHCMELGNLSSLELIEIDGSPSARKLFILWNPIMVLSLAQ--LWTLQGSLQKWFS--- 722
            PRQHCMELG+LSSLELIE DGSPSARKLFILWNPIM    ++  + +LQ S +K  +   
Sbjct: 665  PRQHCMELGSLSSLELIENDGSPSARKLFILWNPIMASKSSERGIDSLQ-STEKNANFRN 724

Query: 723  ----MDFAALLFVKLVNF---VNLFYATRR---------RELLKESAPHLVQSVCAYRAG 782
                MD A  LF ++V        F  TR+         RE+LKE APHLVQSVCAYRAG
Sbjct: 725  PSPIMDIAR-LFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKERAPHLVQSVCAYRAG 784

Query: 783  YTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFP----------GRAGR 842
            YTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFP          GRAGR
Sbjct: 785  YTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQAGRAGR 844

Query: 843  REKTSLSVYVAFEGPLDQYFMKHPEKLFGSPIECCHIDAENQQVLEQHLLCAAYEHPVNV 902
            REKTSLSVYVAFEGPLDQYFMKHPEKLFGSPIECCHIDAENQQVLE HLLCAAYEHPV +
Sbjct: 845  REKTSLSVYVAFEGPLDQYFMKHPEKLFGSPIECCHIDAENQQVLEHHLLCAAYEHPVCM 904

Query: 903  VYDQKFFGPGLNTALISLKSRGDLIPEPSCGSSKSIWNYIGQEKMPSRLVSIRAIETERY 962
            VYDQ  FGPGLNTAL+SLKSRGDL+P PSCGSSKSIW YIG+EKMPSR VSIRAIE ERY
Sbjct: 905  VYDQNLFGPGLNTALVSLKSRGDLMPAPSCGSSKSIWTYIGKEKMPSRSVSIRAIEAERY 964

Query: 963  KVVDQRQNEVLEEIEESTAFFQVYEGAVYLHQGRTYLVKSLNLSTMLAFCEEADLKYYTK 1022
            KVVDQR+NEVLEEIEESTAFFQVYEGAVY+HQGRTYLVKSLNLSTMLAFCEEADLKYYTK
Sbjct: 965  KVVDQRRNEVLEEIEESTAFFQVYEGAVYMHQGRTYLVKSLNLSTMLAFCEEADLKYYTK 1024

Query: 1023 TRDFTDVHVIGA------------------------------------------------ 1044
            TRD+TDVHVIG                                                 
Sbjct: 1025 TRDYTDVHVIGGSLAYPRRAPNIPLLKTTAQANDCRVTTTWFGFYRIWKGSNQIFDTVDL 1084

BLAST of Spg022152 vs. TAIR 10
Match: AT5G08110.1 (nucleic acid binding;ATP-dependent helicases;ATP binding;helicases;ATP-dependent helicases )

HSP 1 Score: 855.9 bits (2210), Expect = 3.4e-248
Identity = 506/1129 (44.82%), Postives = 667/1129 (59.08%), Query Frame = 0

Query: 8    IGIKTLTGESLTVSISGDRTIEDLKLLLRRNFPSATISPNFHLFFKGSKLKPQSQISACR 67
            I ++++ GES TV +S D TI DLK  L+  FP A+ S NFHL+ KG KLK  ++++A  
Sbjct: 15   ISVRSINGESTTVQVSSDGTIRDLKKALKSCFPPASSSSNFHLYIKGEKLKLDTRVAAIT 74

Query: 68   IDNGEFLVLIPFNKKESSKSLLRDQYEQGSSVSGGSSISQFADSAWSDMVQDLSYLHDCS 127
            I++G+ L L PF KKE  ++   D       +S  SS+S              S + D +
Sbjct: 75   INDGDILGLFPFKKKELRQTPKPD-------LSKPSSLS-----------SRTSTMKDEN 134

Query: 128  TRGREDN--GSERERGNSEVGGVDAELVATRSTSSSSSKAKGRKGFVYNGLKGNLDDVLR 187
             +  ED+  G +R+R        D E                  GF  + L+    D  +
Sbjct: 135  AKRAEDHCVGEKRKR--------DEEACP--------------YGFFNDDLESECKDAFK 194

Query: 188  NLLSSPAEGGLSERTCENFVKFLESVDCLSDPRNGKCMLANQVSSRSGNKRAPNRTCGSS 247
                         +  E   + L+S +CL+ P + KC+++   SS             S 
Sbjct: 195  G------------QNTEKLAEVLKSRNCLTSPGSTKCLMSWDSSS-------------SL 254

Query: 248  CLCPGWLKKIMKAFAFLNVFSMFLQLQEENMTVSRLEQAMDLLQKHGITLCMEDMKHLSL 307
            C CP W                               +++  L   G+ + ++D+K+LS+
Sbjct: 255  CSCPDW-------------------------------ESLSRLAMSGVRVRIQDVKNLSV 314

Query: 308  LCPKAVHFASGSLEDTGDDTLIIIISLTEQNGRWKDDNTACKSFKAPTDVTLLERRERSF 367
            +CPK +   +   E    +  I+I    E      D+ +  K        + +++RE SF
Sbjct: 315  ICPKVI---TDDYEAVNYENAIVIADYLEM-----DEKSGRKKIPLAKLFSAMKKREASF 374

Query: 368  KIFLLEAIKSHMLRHGSRSEICVPFSLEDLI--TSKESAVDGNEAKRAKKSETASLSSNS 427
            K    E+I+S + ++   S I +  SLE L+   S+  A  GNEA++A K    +  S  
Sbjct: 375  KSDFWESIRSLLNKNTGESGIAI--SLEGLLKFASEGRADGGNEARQAGKGTCPTSGSRK 434

Query: 428  DRMQCHDTSKLLPENMVEHLVNGVGSEGQIVHIEDIAARKANYVEIPEELSNNVVSALKC 487
             +  CH T+ LLP  MVEHL NG+GS+GQ+VH+E I ARK+ YVE+ ++LS    SALK 
Sbjct: 435  FQTLCHATNSLLPSEMVEHLRNGIGSKGQVVHVEVIKARKSAYVEMSDKLSETTKSALKR 494

Query: 488  IGVAKLYSHQAQSIEASLAGKNVAVATMTSSGKSLCYNLPVLESMSQNVSSCALYLFPTK 547
            IG+  LYSHQA++I A+LAGKNVAVATMTSSGKSLCYN+PV E + ++ +SCALYLFPTK
Sbjct: 495  IGLNTLYSHQAEAISAALAGKNVAVATMTSSGKSLCYNVPVFEELCKDTNSCALYLFPTK 554

Query: 548  ALAQDQLRSLLVMMKGFNDNVDIGVYDGDTSQADRIFLRDNARLLITNPDMLHLSILPHH 607
            ALAQDQLR+L  ++KGF  ++++GVYDGDT   DR  LR NARLLITNPDMLH+SIL  H
Sbjct: 555  ALAQDQLRALSDLIKGFEASINMGVYDGDTPYKDRTRLRSNARLLITNPDMLHISILRRH 614

Query: 608  R-QFSRILSNLRFIVIDEAHTYKGAFGCHTALILRRLCRLCCHVYGSDPSFIFCTATSAN 667
            + QFSRILSNLR+IVIDEAH YKG FGCH ALILRRL RLC HVYG +PSFIFCTATSAN
Sbjct: 615  KEQFSRILSNLRYIVIDEAHIYKGPFGCHMALILRRLRRLCSHVYGVNPSFIFCTATSAN 674

Query: 668  PRQHCMELGNLSSLELIEIDGSPSARKLFILWNP-------------IMVLSLAQLWTLQ 727
            PR+HCMEL NLS LEL+ IDGSPS+ KLF+LWNP             ++  S A      
Sbjct: 675  PREHCMELANLSELELVTIDGSPSSEKLFVLWNPSAPPTSKSEKSSKVVSSSEAAADKPS 734

Query: 728  GSLQKWFSMDFAAL--LFVKLVNF---VNLFYATRR---------RELLKESAPHLVQSV 787
            G+     S   + +  LF ++V        F  +R+         RE+L E+APHLV+++
Sbjct: 735  GAAVDTLSGPASEVSHLFAEMVQHGLRCIAFCPSRKLCELVLCLTREILAETAPHLVEAI 794

Query: 788  CAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFP--------- 847
             +YR GY AEDRR+IESD FGG LCG+AATNALELGIDVGHID TLHLGFP         
Sbjct: 795  TSYRGGYIAEDRRKIESDLFGGKLCGIAATNALELGIDVGHIDVTLHLGFPGSIASLWQQ 854

Query: 848  -GRAGRREKTSLSVYVAFEGPLDQYFMKHPEKLFGSPIECCHIDAENQQVLEQHLLCAAY 907
             GR+GRR+K SL+VYVAF GPLDQY+M  P+KLFGSPIECCHID++N+ VL QHL CAA 
Sbjct: 855  AGRSGRRQKPSLAVYVAFLGPLDQYYMSFPDKLFGSPIECCHIDSQNKHVLMQHLACAAL 914

Query: 908  EHPVNVVYDQKFFGPGLNTALISLKSRGDLIPEPSCGSSKSIWNYIGQEKMPSRLVSIRA 967
            EHP+++ YDQ+ FG GL+  L  L+++G L  +PS  SS  IWNYIG+EK P+R+VSIRA
Sbjct: 915  EHPLSLQYDQQHFGSGLSDPLAELRNKGFLSFDPSRDSSSRIWNYIGREKNPARIVSIRA 974

Query: 968  IETERYKVVDQRQNEVLEEIEESTAFFQVYEGAVYLHQGRTYLVKSLNLSTMLAFCEEAD 1027
            IET RY+V++++  +VL+EIEES AFF VYEGA+Y++QGR YLV SL++   +A CE  +
Sbjct: 975  IETVRYRVMEKKSKDVLDEIEESKAFFHVYEGAIYMNQGRNYLVTSLDIKEKVALCELVN 1034

Query: 1028 LKYYTKTRDFTDVHVIGA------------------------------------------ 1043
            + YYT+TRD+TD+ V G                                           
Sbjct: 1035 VDYYTRTRDYTDIKVTGGDTAYPVKAPKKPTPQTHACRVTTKWFGFLRIRRRNNEVIDDV 1037

BLAST of Spg022152 vs. TAIR 10
Match: AT1G77030.1 (hydrolases, acting on acid anhydrides, in phosphorus-containing anhydrides;ATP-dependent helicases;nucleic acid binding;ATP binding;RNA binding;helicases )

HSP 1 Score: 53.5 bits (127), Expect = 1.2e-06
Identity = 96/408 (23.53%), Postives = 160/408 (39.22%), Query Frame = 0

Query: 473 LSNNVVSALKCIGVAKLYSHQAQSIEASLAGKNVAVATMTSSGKSLCYNLPVLESMSQNV 532
           L  NV +A+K  G       Q +++   L+G +V     T SGK+  + +P+LE + Q+V
Sbjct: 35  LGPNVFNAIKKKGYKVPTPIQRKTMPLILSGVDVVAMARTGSGKTAAFLIPMLEKLKQHV 94

Query: 533 SS---CALYLFPTKALAQDQLRSLLVMMKGFNDNVDIGVYDGDTSQADRIFLRDNARLLI 592
                 AL L PT+ LA+  L+    + K  +  V + +  GD+ +     L     ++I
Sbjct: 95  PQGGVRALILSPTRDLAEQTLKFTKELGKFTDLRVSL-LVGGDSMEDQFEELTKGPDVII 154

Query: 593 TNPDMLHLSILPHHRQFSRILSNLRFIVIDEAHTYKG-AFGCHTALILRRLCRLCCHVYG 652
             P  L + +L      +  L  + ++V DEA +  G  F      IL +L         
Sbjct: 155 ATPGRL-MHLLSEVDDMT--LRTVEYVVFDEADSLFGMGFAEQLHQILTQL--------S 214

Query: 653 SDPSFIFCTATSANPRQHCMELGNLSSLELIEIDG----SPSARKLFILWNPIMVLSLAQ 712
            +   +  +AT  +      + G L   +L+ +D     SP  +  F+   P    S   
Sbjct: 215 ENRQTLLFSATLPSALAEFAKAG-LREPQLVRLDVENKISPDLKLSFLTVRPEEKYS--- 274

Query: 713 LWTLQGSLQKWFSMDFAALLFVKL---VNFVNLFYATRRRELLKESAPHLVQSVCAYRAG 772
              L   +++  S D   L+FV     V FVN  +     E           SVC    G
Sbjct: 275 --ALLYLVREHISSDQQTLIFVSTKHHVEFVNSLFKLENIE----------PSVC---YG 334

Query: 773 YTAEDRRRIE-SDFFGGNLCGVAATNALELGIDVGHIDATLHLGFP----------GRAG 832
              +D R+I  S F       +  T+    GID+  +D  ++  FP          GRA 
Sbjct: 335 DMDQDARKIHVSRFRARKTMLLIVTDIAARGIDIPLLDNVINWDFPPRPKIFVHRVGRAA 394

Query: 833 RREKTSLSVYVAFEGPLDQYFMKHPEKLFGSPIECCHIDAENQQVLEQ 859
           R  +T  +   +F  P D  +M         P+     + E  + +E+
Sbjct: 395 RAGRTGCA--YSFVTPEDMPYMLDLHLFLSKPVRPAPTEDEVLKNMEE 409

BLAST of Spg022152 vs. TAIR 10
Match: AT4G16630.1 (DEA(D/H)-box RNA helicase family protein )

HSP 1 Score: 49.3 bits (116), Expect = 2.2e-05
Identity = 66/316 (20.89%), Postives = 131/316 (41.46%), Query Frame = 0

Query: 380 HGSRSEICVPFSLEDLITSKESAVDGNEAKRAKKSETASLSSNSDRMQCHDTSKLLPENM 439
           H  R    +   +   I  +   +  NE +  ++ E  +  + +D+ + + +     +  
Sbjct: 86  HARRHTTSIDEKISKAIQHRPVPISINEEEEEEEEEEDASDAETDKQEEYLSE---DDEA 145

Query: 440 VEHLVNGVGSEGQIVHIEDIAARKANYVEIPEELSNNVVSALKCIGVAKLYSHQAQSIEA 499
            E+       +     ++ ++     ++E+   LS  ++ A + +G  K    QA  I  
Sbjct: 146 AEYKPEDATPKPFFSTVDGVSFHADTFMEL--NLSRPLLRACETLGYKKPTPIQAACIPL 205

Query: 500 SLAGKNVAVATMTSSGKSLCYNLPVLESM----SQNVSSCALYLFPTKALAQDQLRSLLV 559
           +L G+++  + +T SGK+  + LP LE +     +  ++  L L PT+ LA  Q+ S++ 
Sbjct: 206 ALTGRDLCASAITGSGKTAAFALPTLERLLFRPKRVFATRVLILTPTRELAV-QIHSMIQ 265

Query: 560 MMKGFNDNVDIGVYDGDTS-QADRIFLRDNARLLITNPDMLHLSILPHHRQFSRI-LSNL 619
            +  F D +  G+  G  S +   + LR    +++  P  +    + H R    + L +L
Sbjct: 266 NLAQFTD-IKCGLIVGGLSVREQEVVLRSMPDIVVATPGRM----IDHLRNSMSVDLDDL 325

Query: 620 RFIVIDEAHTYKGAFGCHTALILRRLCRLCCHVYGSDPSFIFCTATSANPRQHCMELGNL 679
             +++DEA           A  +  L RLC       P        SA   +   EL  L
Sbjct: 326 AVLILDEADR---LLQTGFATEITELVRLC-------PKRRQTMLFSATMTEEVKELVKL 380

Query: 680 SSLELIEIDGSPSARK 690
           S  + + +   PSAR+
Sbjct: 386 SLNKPLRLSADPSARR 380

BLAST of Spg022152 vs. TAIR 10
Match: AT2G40700.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 47.0 bits (110), Expect = 1.1e-04
Identity = 38/136 (27.94%), Postives = 66/136 (48.53%), Query Frame = 0

Query: 493 QAQSIEASLAGKNVAVATMTSSGKSLCYNLPVLE-------SMSQNVSSCALYLFPTKAL 552
           QAQ+I   L+G++V V   T +GK++ Y  P++         + ++  + AL + PT+ L
Sbjct: 57  QAQAIPVILSGRDVLVNAPTGTGKTIAYLAPLIHHLQGHSPKVDRSHGTFALVIVPTREL 116

Query: 553 AQDQLRSLLVMMKGFNDNVDIGVYDGDTSQADRIFLRDNARLLITNPDMLHLSILPHHRQ 612
                 +L  ++  F+  V   V  G+    ++  LR    +LI  P  L L  L +   
Sbjct: 117 CLQVYETLEKLLHRFHWIVPGYVMGGEKKAKEKARLRKGISILIATPGRL-LDHLKNTAS 176

Query: 613 FSRILSNLRFIVIDEA 622
           F  +  NLR+++ DEA
Sbjct: 177 F--VHKNLRWVIFDEA 189

BLAST of Spg022152 vs. TAIR 10
Match: AT3G18600.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 45.4 bits (106), Expect = 3.2e-04
Identity = 60/236 (25.42%), Postives = 106/236 (44.92%), Query Frame = 0

Query: 399 KESAVDGNEAKRAKKSETASLSSNSDRMQCHDTSKLL--------PENMVEHLVNGVGSE 458
           K S    NE ++A++ +T ++  N+D  Q     K+          E  VE + +G   +
Sbjct: 18  KRSRGKKNEQQKAEE-KTHTVEENADETQKKSEKKVKKVRGKIEEEEEKVEAMEDGEDEK 77

Query: 459 GQIVHIEDIAARKANYVEIPEELSNNVVSALKCIGVAKLYSHQAQSIEASLAGKNVAVAT 518
             ++  + I     N      +LS     A+K +G   +   QA SI+  L GK+V  A 
Sbjct: 78  NIVIVGKGI---MTNVTFDSLDLSEQTSIAIKEMGFQYMTQIQAGSIQPLLEGKDVLGAA 137

Query: 519 MTSSGKSLCYNLPVLESM-----SQNVSSCALYLFPTKALAQDQLRSLLVMMKGFNDNVD 578
            T SGK+L + +P +E +     S    +  + + PT+ LA         ++K  +  V 
Sbjct: 138 RTGSGKTLAFLIPAVELLFKERFSPRNGTGVIVICPTRELAIQTKNVAEELLKHHSQTVS 197

Query: 579 IGVYDGDTSQADRIFLRDNARLLITNPDMLHLSILPHHRQFSRILSNLRFIVIDEA 622
           + V  G+  +++   +   + L+I  P  L L  L + + F  I  +L+ +VIDEA
Sbjct: 198 M-VIGGNNRRSEAQRIASGSNLVIATPGRL-LDHLQNTKAF--IYKHLKCLVIDEA 245