Spg022144 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg022144
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	isoleucine N-monooxygenase 1-like
Location	scaffold2: 13346661 .. 13350526 (+)
RNA-Seq Expression	Spg022144
Synteny	Spg022144

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCACCCGAGCCTGGACTACCAAGTCTACCTCACCTCAGAGCTCAAACACTTGCTCAAGCACTTCATGTAAGTCAAGCTCACCTGGCGGAGGCCGAGACCAAGCACCTCTTGCCAAAGCCAAGCACCTCTTGTCGAGGCCGAGCACAAACTTTAAAGGTATTCTCCTATTTACTCAACTATACTTTATGTCTTAATGAAGTTTTACTTGAGATTGGGGGCTGCAAAGGGGATATGCACTCCCTATTAAACAAAAAGCAAGTAACAGGGGATATGCACTCCCTATTAAACAAAAGGCACACCAGCTTAGCATATCTAGCCAATGACTACATGTTCTAGCATGTTATTACATTATTTTCCTACAAGGACTAATATATGGATGCATGGAGCTAAGTTTCAGGCTAGCAGGGGTATGAGGCCGAGGCCGAGTCTAGCCTCTTCCCTCATTCTTCTTTTTGCAGAAATTAACTTTTTGATGCAGGACTGTGAGATCTTCCATTTTTCCTACATCTTACTTGTGGATAAGCCCTGGATCCGGAATGATGCACTCCGAAGCAAAATTAGAAAAATGCTCGGCCTCTTCCCGAGGCCGAGGCCGAGCAGGCAAGCTAAAACGGGGATGGCAGTGCTCGGCCTCTTCCCGAGGCCGACCAGCACCACTTTTGTAATCCCTCACCTTCTCTCATTTCTATCTCACCTAACCCCTATTCAGGGAGTTTTGTTTTGTCTATTTCTATCCCATAATGCAGGATAGCAATGAATCCGCACGAAAGTCAGCACAGAGCAAGGATCATTACTCTTAAATCGGGCTTAAGGAGCTCAACCGTGTTGGGATTGGTGTCCTAAAACTCGTAGATAGTGAATGTTAAACGAATTATATACTTATTCGATAAAGGCGTTATCGAGATTAATTCAGTAAAGTTATTACTGAATAAAGTAAATTGCACATATAATAACTCAAATCCAATAAACTAAGATCCATGGCTATAGGATGAACACTTGAGCTTTATGTAGAGACATAAAAGTGGATCAAGCTTGAGTAATAGCCAAAACGGTCTATAGTATAGGGATAGGGTTGGGTACCTTATTCTGGGGACACTATGAGACGCGGCCCACTTTATAGATGGTATAGACGATGTGATCCTAAATCGTCCATGTAGAGACATGCGAGTGGGGGCGTCCTATGCAATGAGTTTGCATAAGACCGGGGCTTGAACAAAGGGATCCCGCCCTCTCATTGGCCCGAGAGGGACTCGGTTTATTGGTTGGACCATAAACCAATTGTTCATTAGAGGATCAGTGGGACTTAAGGAACTAGAAGTACTATAGGGGTAAAACGGCTTATTGACCTAGCTATAGCTACGAACAACCTGTGAAGGATCGACTTACTAATCATGGTTAAATCAAGTGGACGGAAATATATCTATAGTGAGGGGAGTGCAACTATCGGGCTATAGTGGAGTGTCCCGATAGTTAACGAATGTTGGCTAGCTTAGACAATGAGATTAGTCGGTTAGCCTCGAATCGTTGGAGCCCATGATCTATAGGTCCATTAGGTCCCCCTACTAGCTCATATCGGAATTAAGCCTAGAACAAAAGATAGGAAGCTTTGAATCGTTCAAAATCGGGAAAAAGGGTATTATCGTAAATTGTGTTTGACACAATAATTTAATTATGAATTAAATTAGAAAATGAGAGATGAAGTATTTAAAAGTGTTTTGAATACTATATATATGTATTAGGAATCATATATATGGAATTGCACAAGATAACAAAGATTAATTAGATTAATCATATCAAAATTAATTTGAAAATTTTCAAATTAATTACCAAGCAATCAATTTCAATTTAGAATTGAAATTGGGGGTAATTTGCAAGCTTGAAATTTGATTTCAATTTGAATTTAGTGGATTTAGTTGGAAATCCACTTAATGCATGATCATTCAAAGTGGAAAGACAACTCACTCATGCATTGTTGCATGTCTTGTGTAATGTACACCTAGAATCCACTTGAGCTTGCAAATACACCATGTGTCTTGTGTCTTGAGGCTTGGCTTTGCATGGAGAAAGTCTTTAAAAGGAGTTTGAATCATATTCAAACATAAGAGAACCATTTCCTTAATGTTATGCTGCTGGAATTTGGACTCAAAAGTTCTCTCATCTCTCATTCTTCTCCATTGCTTGATGCTTCAAGCATTCCCTTCCCATTCCCTATCTTATATAGACTCCCACAAGTCGTTCTTGGCCCGGAGTATAGTAGGGAAGACTCTAGAGGTTGTCCAAGGAGCATAGGAGAGGCAAACACCACAAGGAGTTCATCATAAGTGAGCTACAAAGGTTGGTGTTTTCTAAACTCTCTATTCTGTCTTTTCTAGAACATGCATGCTTTAGGAGTAAAATTGAATGTCTTAAGTGCTTTCGATCTTCAATTGCTTCCGCATGTGTAATATGTACACCTTCAAACCGATAGCACTAAGTCGCACCTGAATCCCAATTCAAGAGTGGGGGCTTAATGTTATAGGCAATTTTGGACCACCCGACGCACAAGGAGCTGACGAGGACGTCCGGGCGAAAATAGGGCTGGGAGACCGACCCAGAGGAAGAACCGACCATTCGGCCCGCTTGCGCGGGCCGAGCTCGGTCACTTCCTCTCGGTCCCTGATGCTTCTAGCCGCCCCGGTTTCGCCTGGTTTGTCCCGAAACGCCTCCGAATTCCTAAAAACCCTAGGAGCACGAGCAGGTATTTATACCCCTCTTCGCCACTGAAGAAGGGGACCCGAACTCAATTCTCGACTCTCTTCTCTTGCTCTCTAGCTCTCCCTTCCGTTTTCTGACTTAAGCATCGGAGGCGGTGTGGCTAGCACCACACCGGTGTGCAGGTTTTCTCTTTTGCAGGCCACGTCTTCCCCCTCATCTACAAATTTACCGTTGGTGGCACGTGAAGGTCAGGCGAGTTTCCTCTGACCAAAATCGACCATCAACACCTTGATTTTGTATCCAACGGTTATTAGGGGCAGAAAAATTTGAAGATTTGCATCCGAAGATTTTTAGAAGGGAGTCTCATATATTGCACCAGGAATACCTAATAATTTTCCCACAAAATGAGGAGGGAGTCTCATTGCACCAGGAATACCTAATAATTTTCCCACAAAATGAGGAGGGGGCCTCATTGTGGCTGAATCACCTAATAATTTTTTCTGTGGACGCATGTATATATGTTTAATCATTTTCCTAATGACAGGAGTTACATATGGCAACGATTGATAATCCTTCAAACGGAGTGGAATGGGCAATGGCGGAGTTATTCAATCAACCAAAAATTCTCCAACAAGCAATAGAGGAATTGGATTGGATAGTTGGAAAAGACAAACTTGTTCAAGAATCCGACATCCCACAACTTAAGTATCTCATGGCTTGTATCAGAGAATCTTTTCGACTTCATCCATTTTCACCTTTCAATGTGCCTCACGTCTCAAATTCTGATGCCATAGTAGCTGGTTACTTCATTCCAAAAGTAGTCAAGTCATGCTGAGTCGTTCGGGACTCGGTCGAAATCCGAGGATTTGGGACGATCCATTCAGTTACAATCCACAACGCCATCTCAACGATGAGACAATTGAATTAGGATTATCCGAACCGAGTCTACGCTTCATTAGCTTCTTTAGAGGGAGAAGAGGTTGCATAGGTAGCTCACTAGGCACAACCATTACCATGATGCTCTTTGCTAGGTTGCTACAAGGGTTTTCATGGAGCTTAATGCCCGGAACAACTAAGGTAAACTTCTCTCACTTTGATGAACTTGCCCTTCCAAACCCACTCCACTTGTATGCAGAGCCACGTCTATCTCATAATATGTATCCTACTTAG

mRNA sequence

ATGCACCCGAGCCTGGACTACCAAGTCTACCTCACCTCAGAGCTCAAACACTTGCTCAAGCACTTCATGACTGTGAGATCTTCCATTTTTCCTACATCTTACTTGTGGATAAGCCCTGGATCCGGAATGATGCACTCCGAAGCAAAATTAGAAAAATGCTCGGCCTCTTCCCGAGGCCGAGGCCGAGCAGGCAAGCTAAAACGGGGATGGCAGTGCTCGGCCTCTTCCCGAGGCCGACCAGCACCACTTTTGATAGCAATGAATCCGCACGAAAGTCAGCACAGAGCAAGGATCATTACTCTTAAATCGGGCTTAAGGAGCTCAACCGTGTTGGGATTGAACATGCATGCTTTAGGAGTAAAATTGAATGTCTTAAGTGCTTTCGATCTTCAATTGCTTCCGCATGTGGCTGGGAGACCGACCCAGAGGAAGAACCGACCATTCGGCCCGCTTGCGCGGGCCGAGCTCGGTCACTTCCTCTCGGTCCCTGATGCTTCTAGCCGCCCCGGTTTCGCCTGGTTTGTCCCGAAACGCCTCCGAATTCCTAAAAACCCTAGGAGCACGAGCAGCTGGTTACTTCATTCCAAAAGTAGTCAAGTCATGCTGAGTCGTTCGGGACTCGGTCGAAATCCGAGGATTTGGGACGATCCATTCAGTTACAATCCACAACGCCATCTCAACGATGAGACAATTGAATTAGGATTATCCGAACCGAGTCTACGCTTCATTAGCTTCTTTAGAGGGAGAAGAGGTTGCATAGGTAGCTCACTAGGCACAACCATTACCATGATGCTCTTTGCTAGGTTGCTACAAGGGTTTTCATGGAGCTTAATGCCCGGAACAACTAAGGTAAACTTCTCTCACTTTGATGAACTTGCCCTTCCAAACCCACTCCACTTGTATGCAGAGCCACGTCTATCTCATAATATGTATCCTACTTAG

Coding sequence (CDS)

ATGCACCCGAGCCTGGACTACCAAGTCTACCTCACCTCAGAGCTCAAACACTTGCTCAAGCACTTCATGACTGTGAGATCTTCCATTTTTCCTACATCTTACTTGTGGATAAGCCCTGGATCCGGAATGATGCACTCCGAAGCAAAATTAGAAAAATGCTCGGCCTCTTCCCGAGGCCGAGGCCGAGCAGGCAAGCTAAAACGGGGATGGCAGTGCTCGGCCTCTTCCCGAGGCCGACCAGCACCACTTTTGATAGCAATGAATCCGCACGAAAGTCAGCACAGAGCAAGGATCATTACTCTTAAATCGGGCTTAAGGAGCTCAACCGTGTTGGGATTGAACATGCATGCTTTAGGAGTAAAATTGAATGTCTTAAGTGCTTTCGATCTTCAATTGCTTCCGCATGTGGCTGGGAGACCGACCCAGAGGAAGAACCGACCATTCGGCCCGCTTGCGCGGGCCGAGCTCGGTCACTTCCTCTCGGTCCCTGATGCTTCTAGCCGCCCCGGTTTCGCCTGGTTTGTCCCGAAACGCCTCCGAATTCCTAAAAACCCTAGGAGCACGAGCAGCTGGTTACTTCATTCCAAAAGTAGTCAAGTCATGCTGAGTCGTTCGGGACTCGGTCGAAATCCGAGGATTTGGGACGATCCATTCAGTTACAATCCACAACGCCATCTCAACGATGAGACAATTGAATTAGGATTATCCGAACCGAGTCTACGCTTCATTAGCTTCTTTAGAGGGAGAAGAGGTTGCATAGGTAGCTCACTAGGCACAACCATTACCATGATGCTCTTTGCTAGGTTGCTACAAGGGTTTTCATGGAGCTTAATGCCCGGAACAACTAAGGTAAACTTCTCTCACTTTGATGAACTTGCCCTTCCAAACCCACTCCACTTGTATGCAGAGCCACGTCTATCTCATAATATGTATCCTACTTAG

Protein sequence

MHPSLDYQVYLTSELKHLLKHFMTVRSSIFPTSYLWISPGSGMMHSEAKLEKCSASSRGRGRAGKLKRGWQCSASSRGRPAPLLIAMNPHESQHRARIITLKSGLRSSTVLGLNMHALGVKLNVLSAFDLQLLPHVAGRPTQRKNRPFGPLARAELGHFLSVPDASSRPGFAWFVPKRLRIPKNPRSTSSWLLHSKSSQVMLSRSGLGRNPRIWDDPFSYNPQRHLNDETIELGLSEPSLRFISFFRGRRGCIGSSLGTTITMMLFARLLQGFSWSLMPGTTKVNFSHFDELALPNPLHLYAEPRLSHNMYPT

Homology

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BLAST of Spg022144 vs. NCBI nr
Match: KAG6590179.1 (Tryptophan N-monooxygenase CYP79A68, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 197.6 bits (501), Expect = 1.6e-46
Identity = 96/140 (68.57%), Postives = 109/140 (77.86%), Query Frame = 0


Query: 174 FVPKRLRIPKNPRSTSSWLLHSKSSQVMLSRSGLGRNPRIWDDPFSYNPQRHLNDETIEL 233
           F P  L    N   T +     K S+V LSR GLGRNPR W+DP  +NP+RHLND T EL
Sbjct: 383 FSPFNLPHVSNSDITIAGYFIPKGSEVFLSRIGLGRNPRTWEDPMKFNPERHLNDTTTEL 442

Query: 234 GLSEPSLRFISFFRGRRGCIGSSLGTTITMMLFARLLQGFSWSLMPGTTKVNFSHFDELA 293
           GL EP+LRFI+F RGRRGC+GSSLGTTITMMLFARLLQGFSWSL+PG TKV+FS  DEL+
Sbjct: 443 GLLEPNLRFITFSRGRRGCLGSSLGTTITMMLFARLLQGFSWSLLPGITKVDFSDADELS 502

Query: 294 LPNPLHLYAEPRLSHNMYPT 314
           LP PLHL+A+PRLSH MYPT
Sbjct: 503 LPKPLHLHAKPRLSHVMYPT 522

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BLAST of Spg022144 vs. NCBI nr
Match: XP_023516625.1 (isoleucine N-monooxygenase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 196.1 bits (497), Expect = 4.7e-46
Identity = 90/118 (76.27%), Postives = 104/118 (88.14%), Query Frame = 0


Query: 196 KSSQVMLSRSGLGRNPRIWDDPFSYNPQRHLNDETIELGLSEPSLRFISFFRGRRGCIGS 255
           K S+V+LSR GLGRNPR W+DP  +NP+RHLND T E+GL EP+LRFI+F RGRRGC+GS
Sbjct: 405 KGSEVLLSRIGLGRNPRTWEDPMKFNPERHLNDTTTEVGLLEPNLRFITFSRGRRGCLGS 464

Query: 256 SLGTTITMMLFARLLQGFSWSLMPGTTKVNFSHFDELALPNPLHLYAEPRLSHNMYPT 314
           SLGTTITMMLFARLLQGFSWSL+PG TKV+FS  DEL+LP PLHL+A+PRLSH MYPT
Sbjct: 465 SLGTTITMMLFARLLQGFSWSLLPGITKVDFSDADELSLPKPLHLHAKPRLSHVMYPT 522

[Close]

BLAST of Spg022144 vs. NCBI nr
Match: KAG6590174.1 (Isoleucine N-monooxygenase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 194.5 bits (493), Expect = 1.4e-45
Identity = 88/117 (75.21%), Postives = 103/117 (88.03%), Query Frame = 0


Query: 196 KSSQVMLSRSGLGRNPRIWDDPFSYNPQRHLNDETIELGLSEPSLRFISFFRGRRGCIGS 255
           K S+V+LSR+GLGRNPRIW+DP  +NP+RHLND T+ELGL EP+LRF+SF RGRRGC GS
Sbjct: 405 KGSEVLLSRTGLGRNPRIWEDPMRFNPERHLNDRTVELGLLEPNLRFMSFSRGRRGCPGS 464

Query: 256 SLGTTITMMLFARLLQGFSWSLMPGTTKVNFSHFDELALPNPLHLYAEPRLSHNMYP 313
           SLGT ITMMLFARLLQGFSWS +PG TK++FS  DEL+LP PLHL+A+PRLSH MYP
Sbjct: 465 SLGTNITMMLFARLLQGFSWSFVPGITKIDFSEADELSLPKPLHLHAQPRLSHIMYP 521

[Close]

BLAST of Spg022144 vs. NCBI nr
Match: XP_023516711.1 (isoleucine N-monooxygenase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 194.1 bits (492), Expect = 1.8e-45
Identity = 89/117 (76.07%), Postives = 103/117 (88.03%), Query Frame = 0


Query: 196 KSSQVMLSRSGLGRNPRIWDDPFSYNPQRHLNDETIELGLSEPSLRFISFFRGRRGCIGS 255
           K S+V+LSR+GLGRNPRIW+DP  +NP+RH+NDET +LGLSEP+LR ISF RGRRGC GS
Sbjct: 408 KGSEVLLSRTGLGRNPRIWEDPMRFNPERHMNDETTKLGLSEPNLRLISFSRGRRGCPGS 467

Query: 256 SLGTTITMMLFARLLQGFSWSLMPGTTKVNFSHFDELALPNPLHLYAEPRLSHNMYP 313
           SLGT ITMMLFARLLQGFSWSL PG TK++FS  DEL+LP PLHL+A+PRLSH MYP
Sbjct: 468 SLGTNITMMLFARLLQGFSWSLPPGMTKIDFSQADELSLPKPLHLHAQPRLSHIMYP 524

[Close]

BLAST of Spg022144 vs. NCBI nr
Match: XP_023516703.1 (isoleucine N-monooxygenase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 193.0 bits (489), Expect = 4.0e-45
Identity = 88/118 (74.58%), Postives = 103/118 (87.29%), Query Frame = 0


Query: 196 KSSQVMLSRSGLGRNPRIWDDPFSYNPQRHLNDETIELGLSEPSLRFISFFRGRRGCIGS 255
           K S+V+LSR GLGRNP+IW+DP  +NP+RHLND T+ELGL EP+LRFI+F RGRRGC GS
Sbjct: 405 KGSEVLLSRIGLGRNPKIWEDPMKFNPERHLNDRTMELGLLEPNLRFITFSRGRRGCPGS 464

Query: 256 SLGTTITMMLFARLLQGFSWSLMPGTTKVNFSHFDELALPNPLHLYAEPRLSHNMYPT 314
           SLGT ITMMLFARLLQGFSWSL+PG TK++FS  DE +LPNPLHL+A+PRLSH MY T
Sbjct: 465 SLGTNITMMLFARLLQGFSWSLLPGITKIDFSETDEFSLPNPLHLHAKPRLSHVMYST 522

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BLAST of Spg022144 vs. ExPASy Swiss-Prot
Match: A0A068Q7V0 (Phenylalanine N-monooxygenase CYP79D16 OS=Prunus mume OX=102107 GN=CYP79D16 PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 8.3e-30
Identity = 63/119 (52.94%), Postives = 83/119 (69.75%), Query Frame = 0


Query: 196 KSSQVMLSRSGLGRNPRIWDDPFSYNPQRHLNDETIELGLSEPSLRFISFFRGRRGCIGS 255
           K S VMLSR GLGRNP+IWD+P  Y P+RHL D+   + L+E  LRFISF  G RGC+ S
Sbjct: 416 KGSHVMLSRIGLGRNPKIWDEPLKYKPERHLKDDGSGVVLTESELRFISFSTGMRGCVAS 475

Query: 256 SLGTTITMMLFARLLQGFSWSLMPGTTKVNFSHFD-ELALPNPLHLYAEPRLSHNMYPT 314
           +LGT++T+MLFARLL GF+W   P  ++++ +  D EL L  PL   A+PRL  ++Y T
Sbjct: 476 TLGTSMTVMLFARLLHGFTWEAPPNESRIDLTEADGELLLAKPLFALAKPRLPAHVYQT 534

[Close]

BLAST of Spg022144 vs. ExPASy Swiss-Prot
Match: Q6J540 (Isoleucine N-monooxygenase 2 OS=Lotus japonicus OX=34305 GN=CYP79D4 PE=1 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 9.2e-29
Identity = 63/122 (51.64%), Postives = 85/122 (69.67%), Query Frame = 0


Query: 193 LHSKSSQVMLSRSGLGRNPRIWDDPFSYNPQRHLNDETIELGLSEPSLRFISFFRGRRGC 252
           L  K +QV+LSR  LGRNP+ W DP  +NP+RHL  E I++ L+EP LRFISF  GRR C
Sbjct: 412 LFPKGTQVLLSRVALGRNPKFWTDPLKFNPERHLK-EGIDVVLTEPDLRFISFTTGRRSC 471

Query: 253 IGSSLGTTITMMLFARLLQGFSWSLMPGTTKVNFSH-FDELALPNPLHLYAEPRLSHNMY 312
            G +LGTT+T+MLFAR+L GFSWS  P  + ++ +   D+L +  PL + A+PRL+  +Y
Sbjct: 472 PGVTLGTTMTIMLFARMLHGFSWSAPPNVSSIDLTQSSDDLFMAKPLCVVAKPRLAAELY 531

Query: 313 PT 314
            T
Sbjct: 532 ST 532

[Close]

BLAST of Spg022144 vs. ExPASy Swiss-Prot
Match: Q43135 (Tyrosine N-monooxygenase OS=Sorghum bicolor OX=4558 GN=CYP79A1 PE=1 SV=3)

HSP 1 Score: 128.6 bits (322), Expect = 1.2e-28
Identity = 62/119 (52.10%), Postives = 82/119 (68.91%), Query Frame = 0


Query: 196 KSSQVMLSRSGLGRNPRIWDDPFSYNPQRHLNDETIELGLSEPSLRFISFFRGRRGCIGS 255
           K S V+LSR+GLGRNPR+WD+P  + P RHL     ++ L+E  LRFISF  GRRGCI +
Sbjct: 437 KGSHVILSRTGLGRNPRVWDEPLRFYPDRHLATAASDVALTENDLRFISFSTGRRGCIAA 496

Query: 256 SLGTTITMMLFARLLQGFSWSLMPGTTKVNFSHF-DELALPNPLHLYAEPRLSHNMYPT 314
           SLGT +++MLF RLLQGF+WS   G   V+ S    +  +  PL L+AEPRL  ++YP+
Sbjct: 497 SLGTAMSVMLFGRLLQGFTWSKPAGVEAVDLSESKSDTFMATPLVLHAEPRLPAHLYPS 555

[Close]

BLAST of Spg022144 vs. ExPASy Swiss-Prot
Match: Q6J541 (Isoleucine N-monooxygenase 1 OS=Lotus japonicus OX=34305 GN=CYP79D3 PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 2.0e-28
Identity = 65/122 (53.28%), Postives = 84/122 (68.85%), Query Frame = 0


Query: 193 LHSKSSQVMLSRSGLGRNPRIWDDPFSYNPQRHLNDETIELGLSEPSLRFISFFRGRRGC 252
           L  K +QV+LSR  LGRNP+ W DP  +NP+RHL  E I++ L+EP LRFISF  GRR C
Sbjct: 412 LFPKGTQVLLSRVALGRNPKFWTDPLKFNPERHLK-EGIDVVLTEPDLRFISFTTGRRSC 471

Query: 253 IGSSLGTTITMMLFARLLQGFSWSLMPGTTKVNF-SHFDELALPNPLHLYAEPRLSHNMY 312
            G +LGTT+T+MLFAR+L GFSWS  P  + ++     D+L L  PL L A+PRL+  +Y
Sbjct: 472 PGVALGTTMTVMLFARMLHGFSWSPPPDVSSIDLVPSKDDLFLAKPLLLVAKPRLAAELY 531

Query: 313 PT 314
            T
Sbjct: 532 RT 532

[Close]

BLAST of Spg022144 vs. ExPASy Swiss-Prot
Match: A0A068Q605 (Tryptophan N-monooxygenase CYP79A68 OS=Prunus mume OX=102107 GN=CYP79A68 PE=1 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 2.3e-27
Identity = 64/122 (52.46%), Postives = 86/122 (70.49%), Query Frame = 0


Query: 196 KSSQVMLSRSGLGRNPRIWDDPFSYNPQRHLN---DETIELGLSEPSLRFISFFRGRRGC 255
           K S V+LSR  LGRNPR+W++P  +NP+RHLN   D+ ++  L E  LRF+SF  GRRGC
Sbjct: 414 KGSNVILSRLELGRNPRVWEEPLRFNPERHLNIAVDQQVD--LEENDLRFVSFSTGRRGC 473

Query: 256 IGSSLGTTITMMLFARLLQGFSWSLMPGTTKVNFSHFDELAL--PNPLHLYAEPRLSHNM 313
           +G  LG+TI +ML ARLLQGFSWSL P   K++F+  D++ L   +PL   A+PRL  ++
Sbjct: 474 MGVGLGSTIVVMLLARLLQGFSWSLPPDVDKIDFTE-DQIYLKKASPLLAQAKPRLPASV 532

[Close]

BLAST of Spg022144 vs. ExPASy TrEMBL
Match: A0A6J1D6G9 (isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017498 PE=3 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 2.5e-45
Identity = 89/118 (75.42%), Postives = 102/118 (86.44%), Query Frame = 0


Query: 196 KSSQVMLSRSGLGRNPRIWDDPFSYNPQRHLNDETIELGLSEPSLRFISFFRGRRGCIGS 255
           K S+V+LSRSGLGRNPRIW+DP  ++P+RHL DET+ELGLSEP+LRFISF RGRRGC+GS
Sbjct: 423 KGSEVILSRSGLGRNPRIWEDPMRFDPERHLKDETVELGLSEPNLRFISFTRGRRGCVGS 482

Query: 256 SLGTTITMMLFARLLQGFSWSLMPGTTKVNFSHFDELALPNPLHLYAEPRLSHNMYPT 314
           SLGT ITMML  RLLQGFSWSL  GTTK+ FS  DEL+LP PLHL+A+PRL H MYPT
Sbjct: 483 SLGTNITMMLLGRLLQGFSWSLPFGTTKIEFSEADELSLPKPLHLHAKPRLPHIMYPT 540

[Close]

BLAST of Spg022144 vs. ExPASy TrEMBL
Match: A0A6J1H7W7 (isoleucine N-monooxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111461341 PE=3 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 5.6e-45
Identity = 88/117 (75.21%), Postives = 102/117 (87.18%), Query Frame = 0


Query: 196 KSSQVMLSRSGLGRNPRIWDDPFSYNPQRHLNDETIELGLSEPSLRFISFFRGRRGCIGS 255
           K S+V+LSR+GLGRNPRIW+DP  +N +RHLND T+ELGL EP+LRF+SF RGRRGC GS
Sbjct: 405 KGSEVLLSRTGLGRNPRIWEDPMRFNRERHLNDRTVELGLLEPNLRFMSFSRGRRGCPGS 464

Query: 256 SLGTTITMMLFARLLQGFSWSLMPGTTKVNFSHFDELALPNPLHLYAEPRLSHNMYP 313
           SLGT ITMMLFARLLQGFSWSL+PG TK++FS  DEL+LP PLHL A+PRLSH MYP
Sbjct: 465 SLGTNITMMLFARLLQGFSWSLLPGITKIDFSQADELSLPKPLHLLAKPRLSHVMYP 521

[Close]

BLAST of Spg022144 vs. ExPASy TrEMBL
Match: A0A6J1H9G3 (isoleucine N-monooxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111461343 PE=3 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 7.3e-45
Identity = 91/139 (65.47%), Postives = 106/139 (76.26%), Query Frame = 0


Query: 174 FVPKRLRIPKNPRSTSSWLLHSKSSQVMLSRSGLGRNPRIWDDPFSYNPQRHLNDETIEL 233
           F P  L    N   T +     K S+V LSR GLGRNPR W+DP  +NP+RH+ D T EL
Sbjct: 383 FSPFNLPHVSNSDITIAGYFIPKGSEVFLSRIGLGRNPRTWEDPMKFNPERHMKDTTTEL 442

Query: 234 GLSEPSLRFISFFRGRRGCIGSSLGTTITMMLFARLLQGFSWSLMPGTTKVNFSHFDELA 293
           GL EP+LRFI+F RGRRGC+GSSLGTTITMMLFARLLQGFSWSL+PG TK++FS  DEL+
Sbjct: 443 GLLEPNLRFITFSRGRRGCLGSSLGTTITMMLFARLLQGFSWSLLPGITKIDFSEADELS 502

Query: 294 LPNPLHLYAEPRLSHNMYP 313
           LP PLHL+ +PRLSH MYP
Sbjct: 503 LPKPLHLHGKPRLSHVMYP 521

[Close]

BLAST of Spg022144 vs. ExPASy TrEMBL
Match: A0A6J1H9V7 (isoleucine N-monooxygenase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111461429 PE=3 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 7.3e-45
Identity = 87/117 (74.36%), Postives = 101/117 (86.32%), Query Frame = 0


Query: 196 KSSQVMLSRSGLGRNPRIWDDPFSYNPQRHLNDETIELGLSEPSLRFISFFRGRRGCIGS 255
           K S+V+LSR  LGRNPRIW+DP  +NP+RHLND T+ELGL EP+LRF+SF RGRRGC GS
Sbjct: 405 KGSEVLLSRISLGRNPRIWEDPMRFNPERHLNDRTVELGLLEPNLRFMSFSRGRRGCPGS 464

Query: 256 SLGTTITMMLFARLLQGFSWSLMPGTTKVNFSHFDELALPNPLHLYAEPRLSHNMYP 313
           SLGT ITMMLFARLLQGFSWS +PG TK++FS  DEL+LP PLHL+A+PRLSH MYP
Sbjct: 465 SLGTNITMMLFARLLQGFSWSFVPGITKIDFSEADELSLPKPLHLHAQPRLSHIMYP 521

[Close]

BLAST of Spg022144 vs. ExPASy TrEMBL
Match: A0A6J1DNF2 (isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111021625 PE=3 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 1.4e-43
Identity = 91/138 (65.94%), Postives = 107/138 (77.54%), Query Frame = 0


Query: 174 FVPKRLRIPKNPRSTSSWLLHSKSSQVMLSRSGLGRNPRIWDDPFSYNPQRHLNDETIEL 233
           F P  L    N     +  L  K S+V+LSRSGLGRNPRIW+DP  ++P+RHL D+T+EL
Sbjct: 399 FSPFNLPHVSNSDIVVAGYLIPKGSEVLLSRSGLGRNPRIWEDPMRFDPERHLKDKTVEL 458

Query: 234 GLSEPSLRFISFFRGRRGCIGSSLGTTITMMLFARLLQGFSWSLMPGTTKVNFSHFDELA 293
           GLSEPSLRFI+F RGRRGCIGSSLGTTITMMLFAR+LQ FSWSL PG T ++FS  DEL+
Sbjct: 459 GLSEPSLRFITFTRGRRGCIGSSLGTTITMMLFARMLQAFSWSLPPGMTMIDFSESDELS 518

Query: 294 LPNPLHLYAEPRLSHNMY 312
           LP PL+L+AE RLS  MY
Sbjct: 519 LPKPLYLFAEARLSSTMY 536

[Close]

BLAST of Spg022144 vs. TAIR 10
Match: AT2G22330.1 (cytochrome P450, family 79, subfamily B, polypeptide 3 )

HSP 1 Score: 124.4 bits (311), Expect = 1.6e-28
Identity = 63/118 (53.39%), Postives = 78/118 (66.10%), Query Frame = 0


Query: 196 KSSQVMLSRSGLGRNPRIWDDPFSYNPQRHLNDETIELGLSEPSLRFISFFRGRRGCIGS 255
           K SQV+LSR GLGRNP++W DP S+ P+RHLN E  E+ L+E  LRFISF  G+RGC   
Sbjct: 424 KGSQVLLSRYGLGRNPKVWSDPLSFKPERHLN-ECSEVTLTENDLRFISFSTGKRGCAAP 483

Query: 256 SLGTTITMMLFARLLQGFSWSLMPGTTKVNFSHFD-ELALPNPLHLYAEPRLSHNMYP 313
           +LGT IT M+ ARLLQGF W L    T+V       ++ L  PL L  E RLS ++YP
Sbjct: 484 ALGTAITTMMLARLLQGFKWKLAGSETRVELMESSHDMFLSKPLVLVGELRLSEDLYP 540

[Close]

BLAST of Spg022144 vs. TAIR 10
Match: AT1G79370.1 (cytochrome P450, family 79, subfamily C, polypeptide 1 )

HSP 1 Score: 120.2 bits (300), Expect = 3.0e-27
Identity = 58/119 (48.74%), Postives = 79/119 (66.39%), Query Frame = 0


Query: 196 KSSQVMLSRSGLGRNPRIWDDPFSYNPQRHLNDETIELGLSEPSLRFISFFRGRRGCIGS 255
           K S +++SR G+GRNP+ WD+P  Y P+RH+     E+ L+EP LR +SF  GRRGC+G+
Sbjct: 423 KGSHILVSRPGVGRNPKTWDEPLIYRPERHITGN--EVVLTEPDLRLVSFGTGRRGCVGA 482

Query: 256 SLGTTITMMLFARLLQGFSWSLMPGTT-KVNFSHFDE-LALPNPLHLYAEPRLSHNMYP 313
            LGT++ + L  RLLQGF W++ PGTT +V      E L + NPL    +PRL  NMYP
Sbjct: 483 KLGTSMIVTLLGRLLQGFDWTIPPGTTDRVELVESKENLFMANPLMACVKPRLDPNMYP 539

[Close]

BLAST of Spg022144 vs. TAIR 10
Match: AT4G39950.1 (cytochrome P450, family 79, subfamily B, polypeptide 2 )

HSP 1 Score: 119.4 bits (298), Expect = 5.2e-27
Identity = 59/119 (49.58%), Postives = 78/119 (65.55%), Query Frame = 0


Query: 196 KSSQVMLSRSGLGRNPRIWDDPFSYNPQRHLNDETIELGLSEPSLRFISFFRGRRGCIGS 255
           K SQV+LSR GLGRNP++W DP  + P+RHLN E  E+ L+E  LRFISF  G+RGC   
Sbjct: 422 KGSQVLLSRYGLGRNPKVWADPLCFKPERHLN-ECSEVTLTENDLRFISFSTGKRGCAAP 481

Query: 256 SLGTTITMMLFARLLQGFSWSLMPGTTKVNFSHFD-ELALPNPLHLYAEPRLSHNMYPT 314
           +LGT +T M+ ARLLQGF+W L    T+V       ++ L  PL +  + RL  ++YPT
Sbjct: 482 ALGTALTTMMLARLLQGFTWKLPENETRVELMESSHDMFLAKPLVMVGDLRLPEHLYPT 539

[Close]

BLAST of Spg022144 vs. TAIR 10
Match: AT5G05260.1 (cytochrome p450 79a2 )

HSP 1 Score: 119.0 bits (297), Expect = 6.8e-27
Identity = 55/119 (46.22%), Postives = 81/119 (68.07%), Query Frame = 0


Query: 196 KSSQVMLSRSGLGRNPRIWDDPFSYNPQRHLNDETIELGLSEPSLRFISFFRGRRGCIGS 255
           K S V++SR G+GRNP +WD P  ++P+RHL+  T  + L+E  L  ISF  GRRGC+G 
Sbjct: 406 KGSHVLISRMGIGRNPSVWDKPHKFDPERHLSTNTC-VDLNESDLNIISFSAGRRGCMGV 465

Query: 256 SLGTTITMMLFARLLQGFSWSLMPGTTKVNFSHF-DELALPNPLHLYAEPRLSHNMYPT 314
            +G+ +T ML ARL+QGF+W  +PG  K++ S   ++L +  PL+  A PRL+ ++YPT
Sbjct: 466 DIGSAMTYMLLARLIQGFTWLPVPGKNKIDISESKNDLFMAKPLYAVATPRLAPHVYPT 523

[Close]

BLAST of Spg022144 vs. TAIR 10
Match: AT1G16410.1 (cytochrome p450 79f1 )

HSP 1 Score: 117.1 bits (292), Expect = 2.6e-26
Identity = 58/120 (48.33%), Postives = 75/120 (62.50%), Query Frame = 0


Query: 196 KSSQVMLSRSGLGRNPRIWDDPFSYNPQRHLNDE--TIELGLSEPSLRFISFFRGRRGCI 255
           K S + + R GLGRNP+IW DP  Y P+RHL  +  T E+ L E  +RF+SF  GRRGCI
Sbjct: 415 KGSHIHVCRPGLGRNPKIWKDPLVYKPERHLQGDGITKEVTLVETEMRFVSFSTGRRGCI 474

Query: 256 GSSLGTTITMMLFARLLQGFSWSLMPGTTKVNFSHFD-ELALPNPLHLYAEPRLSHNMYP 313
           G  +GT + +ML AR LQGF+W L      ++    D  L +  PLHL  EPRL+ N+YP
Sbjct: 475 GVKVGTIMMVMLLARFLQGFNWKLHQDFGPLSLEEDDASLLMAKPLHLSVEPRLAPNLYP 534

The following BLAST results are available for this feature:

BLAST of Spg022144 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6590179.1	1.6e-46	68.57	Tryptophan N-monooxygenase CYP79A68, partial [Cucurbita argyrosperma subsp. soro...	[more]
XP_023516625.1	4.7e-46	76.27	isoleucine N-monooxygenase 1-like [Cucurbita pepo subsp. pepo]	[more]
KAG6590174.1	1.4e-45	75.21	Isoleucine N-monooxygenase 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023516711.1	1.8e-45	76.07	isoleucine N-monooxygenase 2-like [Cucurbita pepo subsp. pepo]	[more]
XP_023516703.1	4.0e-45	74.58	isoleucine N-monooxygenase 2-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Spg022144 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A068Q7V0	8.3e-30	52.94	Phenylalanine N-monooxygenase CYP79D16 OS=Prunus mume OX=102107 GN=CYP79D16 PE=1...	[more]
Q6J540	9.2e-29	51.64	Isoleucine N-monooxygenase 2 OS=Lotus japonicus OX=34305 GN=CYP79D4 PE=1 SV=1	[more]
Q43135	1.2e-28	52.10	Tyrosine N-monooxygenase OS=Sorghum bicolor OX=4558 GN=CYP79A1 PE=1 SV=3	[more]
Q6J541	2.0e-28	53.28	Isoleucine N-monooxygenase 1 OS=Lotus japonicus OX=34305 GN=CYP79D3 PE=1 SV=1	[more]
A0A068Q605	2.3e-27	52.46	Tryptophan N-monooxygenase CYP79A68 OS=Prunus mume OX=102107 GN=CYP79A68 PE=1 SV...	[more]

BLAST of Spg022144 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D6G9	2.5e-45	75.42	isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017498...	[more]
A0A6J1H7W7	5.6e-45	75.21	isoleucine N-monooxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111461341 ...	[more]
A0A6J1H9G3	7.3e-45	65.47	isoleucine N-monooxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111461343 ...	[more]
A0A6J1H9V7	7.3e-45	74.36	isoleucine N-monooxygenase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111461429 ...	[more]
A0A6J1DNF2	1.4e-43	65.94	isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111021625...	[more]

BLAST of Spg022144 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G22330.1	1.6e-28	53.39	cytochrome P450, family 79, subfamily B, polypeptide 3	[more]
AT1G79370.1	3.0e-27	48.74	cytochrome P450, family 79, subfamily C, polypeptide 1	[more]
AT4G39950.1	5.2e-27	49.58	cytochrome P450, family 79, subfamily B, polypeptide 2	[more]
AT5G05260.1	6.8e-27	46.22	cytochrome p450 79a2	[more]
AT1G16410.1	2.6e-26	48.33	cytochrome p450 79f1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 197..298 e-value: 1.2E-16 score: 60.6
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 172..310 e-value: 2.5E-18 score: 67.8
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 196..305
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 195..310
None	No IPR available	PANTHER	PTHR24298:SF675	PHENYLALANINE N-MONOOXYGENASE	coord: 195..310

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg022144.1	Spg022144.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen