Spg022103 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg022103 vs. NCBI nr
Match: XP_022931586.1 (probable acyl-activating enzyme 17, peroxisomal [Cucurbita moschata])

HSP 1 Score: 1365.9 bits (3534), Expect = 0.0e+00
Identity = 659/725 (90.90%), Postives = 695/725 (95.86%), Query Frame = 0

Query: 220 MDYRTLDSITLTDIEALGIASGVAEKLHKLLTEIIHSYGNGTPETWRHISKRVLSPDLPF 279
           MDYR LDSIT+TDI+ALGIAS VA++LH +LT+II SYGNG+PETW HISKRVLSPDLPF
Sbjct: 1   MDYRALDSITVTDIKALGIASEVADELHGILTDIIRSYGNGSPETWCHISKRVLSPDLPF 60

Query: 280 SFHQMMYYGCYKHYGLDPPAWMPEPENAVFTNVGQLLERRGKEFLGSNYRDPLSSFSSFQ 339
           SFHQMMYYGCYKHYG DPPAWMPE E+AVFTNVGQLLERRGKEFLGSNYRDP++SFSSFQ
Sbjct: 61  SFHQMMYYGCYKHYGSDPPAWMPELESAVFTNVGQLLERRGKEFLGSNYRDPITSFSSFQ 120

Query: 340 EFSVSNPEVYWRTVLDEMHITFSKPPKCILQMNGSPESQFPNPGGQWLPGGVFNPAKDCL 399
           EFSVSNPEVYW+ VLD+MHITFSKPPKCILQMN SP+ Q P PGGQWLPG VFNPAKDCL
Sbjct: 121 EFSVSNPEVYWKAVLDDMHITFSKPPKCILQMNDSPDCQSPCPGGQWLPGAVFNPAKDCL 180

Query: 400 SVNENRSLDDIAIIWRDEGCDNLPVKRLTLRELRAEVWLIAHALNSIGFEKGTAIAIDMP 459
           S+NENRSLDD A+IWRDEGCDN+PVKRLTLRELR E WL+AHALNS+GFEKGTAIAIDMP
Sbjct: 181 SLNENRSLDDAAVIWRDEGCDNMPVKRLTLRELRTEAWLVAHALNSLGFEKGTAIAIDMP 240

Query: 460 MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRVNISKAKAIFTQDLIIRGDKSIPLYS 519
           MNV +VVIYLAIVLAGHVVVSIADSFAAREISTRVNISKAKAIFTQDLIIRGDKSI LYS
Sbjct: 241 MNVTSVVIYLAIVLAGHVVVSIADSFAAREISTRVNISKAKAIFTQDLIIRGDKSIHLYS 300

Query: 520 RVVDAQSLLAIVIPTRSTGFSMKLRDGDISWHAFLERVKDLKGVEFSAVEQSAESFTNIL 579
           RVVDAQS LAIVIPTRSTG SMKLRDGDISWHAFLERVKDLKGVEF+AVEQSAESFTNIL
Sbjct: 301 RVVDAQSPLAIVIPTRSTGLSMKLRDGDISWHAFLERVKDLKGVEFAAVEQSAESFTNIL 360

Query: 580 FSSGTTGEPKAIPWTIVTPLKAAADAWCHMDIHKGDVVAWPTNLGWMMGPWLVYASLLNS 639
           FSSGTTGEPKAIPWT+VTPLKAAADAWCHMDIHKGDVVAWPTNLGWMMGPWLVYASLLN 
Sbjct: 361 FSSGTTGEPKAIPWTLVTPLKAAADAWCHMDIHKGDVVAWPTNLGWMMGPWLVYASLLNK 420

Query: 640 ASLALYNGSPLGSGFVKFVQDAKITMLGVVPSIVRTWKITNCTSGYDWSSIRCFSSTGEA 699
           ASLALY+GSPLGSGFVKFVQDAK+TMLGVVPSIVR+WK TNCTSGYDWSSIRCFSSTGEA
Sbjct: 421 ASLALYDGSPLGSGFVKFVQDAKVTMLGVVPSIVRSWKSTNCTSGYDWSSIRCFSSTGEA 480

Query: 700 STVDECLWLMGRAGYKPVIEICGGTEIGGGFVAGSLLQPQALSAFSTPGMGCSLFILGND 759
           STVDECLWLMGRAGYKPVIEICGGTEIGGGFVAGSLLQ QALSAFSTPGMGCSLFILGND
Sbjct: 481 STVDECLWLMGRAGYKPVIEICGGTEIGGGFVAGSLLQAQALSAFSTPGMGCSLFILGND 540

Query: 760 GFPIPQHMPGIGELALGPFLFGASSTLLNADHYDIYFKGMPRWNGMVLRRHGDVFERSPR 819
           GFP+PQ+MPGIGELALGPFLFGASSTLLN+DHYD+YFKGMPRWNGMVLRRHGDVFERSPR
Sbjct: 541 GFPLPQNMPGIGELALGPFLFGASSTLLNSDHYDVYFKGMPRWNGMVLRRHGDVFERSPR 600

Query: 820 GYYRAHGRADDTMNLGGIKVSSVEIERVCNTVDDSILETAAIGVPPHGGGPEQLVIAIVF 879
           GYYRAHGRADDTMNLGGIKVSSVEIER+CN+VD+SILETAAIGVPPHGGGPEQL IAIV 
Sbjct: 601 GYYRAHGRADDTMNLGGIKVSSVEIERICNSVDESILETAAIGVPPHGGGPEQLTIAIVS 660

Query: 880 KNPNETSPDLNKLKLSFNSSLQKNLNPLFRVHRVVPYPSLPRTATNKVMRRILRQQLALE 939
           KN + ++PDLNKLKL FNSSLQKNLNPLF+VHRVVPYPSLPRTATNKVMRRILRQQLA +
Sbjct: 661 KNQSGSTPDLNKLKLCFNSSLQKNLNPLFKVHRVVPYPSLPRTATNKVMRRILRQQLAAQ 720

Query: 940 HRPKL 945
           HR KL
Sbjct: 721 HRSKL 725

BLAST of Spg022103 vs. NCBI nr
Match: KAG7021597.1 (putative acyl-activating enzyme 17, peroxisomal [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1364.7 bits (3531), Expect = 0.0e+00
Identity = 658/725 (90.76%), Postives = 695/725 (95.86%), Query Frame = 0

Query: 220 MDYRTLDSITLTDIEALGIASGVAEKLHKLLTEIIHSYGNGTPETWRHISKRVLSPDLPF 279
           MDYR LDSIT+TDI+ALGIAS VA++LH +LT+II SYGNG+PETW HISKRVLSPDLPF
Sbjct: 1   MDYRALDSITVTDIKALGIASEVADELHGILTDIIRSYGNGSPETWCHISKRVLSPDLPF 60

Query: 280 SFHQMMYYGCYKHYGLDPPAWMPEPENAVFTNVGQLLERRGKEFLGSNYRDPLSSFSSFQ 339
           SFHQMMYYGCYKHYG DPPAWMPE E+AVFTNVGQLLERRGKEFLGSNYRDP++SFSSFQ
Sbjct: 61  SFHQMMYYGCYKHYGSDPPAWMPELESAVFTNVGQLLERRGKEFLGSNYRDPITSFSSFQ 120

Query: 340 EFSVSNPEVYWRTVLDEMHITFSKPPKCILQMNGSPESQFPNPGGQWLPGGVFNPAKDCL 399
           EFSVSNPEVYW+ VLD+MHITFSKPPKCILQMN SP+ Q P PGGQWLPG VFNPAKDCL
Sbjct: 121 EFSVSNPEVYWKAVLDDMHITFSKPPKCILQMNDSPDCQSPCPGGQWLPGAVFNPAKDCL 180

Query: 400 SVNENRSLDDIAIIWRDEGCDNLPVKRLTLRELRAEVWLIAHALNSIGFEKGTAIAIDMP 459
           S+NENRSLDD A+IWRDEGCDN+PVKRLTLRELR E WL+AHALNS+GFEKGTAIAIDMP
Sbjct: 181 SLNENRSLDDAAVIWRDEGCDNMPVKRLTLRELRTEAWLVAHALNSLGFEKGTAIAIDMP 240

Query: 460 MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRVNISKAKAIFTQDLIIRGDKSIPLYS 519
           MNV +VVIYLAIVLAGHVVVSIADSFAAREISTRVNISKAKAIFTQDLIIRGDKSI LYS
Sbjct: 241 MNVTSVVIYLAIVLAGHVVVSIADSFAAREISTRVNISKAKAIFTQDLIIRGDKSIHLYS 300

Query: 520 RVVDAQSLLAIVIPTRSTGFSMKLRDGDISWHAFLERVKDLKGVEFSAVEQSAESFTNIL 579
           RVVDA+S LAIVIPTRSTG SMKLRDGDISWHAFLERVKDLKGVEF+AVEQSAESFTNIL
Sbjct: 301 RVVDAESPLAIVIPTRSTGLSMKLRDGDISWHAFLERVKDLKGVEFAAVEQSAESFTNIL 360

Query: 580 FSSGTTGEPKAIPWTIVTPLKAAADAWCHMDIHKGDVVAWPTNLGWMMGPWLVYASLLNS 639
           FSSGTTGEPKAIPWT+VTPLKAAADAWCHMDIHKGDVVAWPTNLGWMMGPWLVYASLLN 
Sbjct: 361 FSSGTTGEPKAIPWTLVTPLKAAADAWCHMDIHKGDVVAWPTNLGWMMGPWLVYASLLNK 420

Query: 640 ASLALYNGSPLGSGFVKFVQDAKITMLGVVPSIVRTWKITNCTSGYDWSSIRCFSSTGEA 699
           ASLALY+GSPLGSGFVKFVQDAK+TMLGVVPSIVR+WK TNCTSGYDWSSIRCFSSTGEA
Sbjct: 421 ASLALYDGSPLGSGFVKFVQDAKVTMLGVVPSIVRSWKSTNCTSGYDWSSIRCFSSTGEA 480

Query: 700 STVDECLWLMGRAGYKPVIEICGGTEIGGGFVAGSLLQPQALSAFSTPGMGCSLFILGND 759
           STVDECLWLMGRAGYKPVIEICGGTEIGGGFVAGSLLQ QALSAFSTPGMGCSLFILGND
Sbjct: 481 STVDECLWLMGRAGYKPVIEICGGTEIGGGFVAGSLLQAQALSAFSTPGMGCSLFILGND 540

Query: 760 GFPIPQHMPGIGELALGPFLFGASSTLLNADHYDIYFKGMPRWNGMVLRRHGDVFERSPR 819
           GFP+PQ+MPGIGELALGPFLFGASSTLLN+DHYD+YFKGMPRWNGMVLRRHGDVFERSPR
Sbjct: 541 GFPLPQNMPGIGELALGPFLFGASSTLLNSDHYDVYFKGMPRWNGMVLRRHGDVFERSPR 600

Query: 820 GYYRAHGRADDTMNLGGIKVSSVEIERVCNTVDDSILETAAIGVPPHGGGPEQLVIAIVF 879
           GYYRAHGRADDTMNLGGIKVSSVEIER+CN+VD+SILETAAIGVPPHGGGPEQL IAIV 
Sbjct: 601 GYYRAHGRADDTMNLGGIKVSSVEIERICNSVDESILETAAIGVPPHGGGPEQLTIAIVS 660

Query: 880 KNPNETSPDLNKLKLSFNSSLQKNLNPLFRVHRVVPYPSLPRTATNKVMRRILRQQLALE 939
           KN + ++PDLNKLKL FNSSLQKNLNPLF+VHRVVPYPSLPRTATNKVMRRILRQQLA +
Sbjct: 661 KNQSGSTPDLNKLKLCFNSSLQKNLNPLFKVHRVVPYPSLPRTATNKVMRRILRQQLAAQ 720

Query: 940 HRPKL 945
           HR KL
Sbjct: 721 HRSKL 725

BLAST of Spg022103 vs. NCBI nr
Match: XP_022135246.1 (probable acyl-activating enzyme 17, peroxisomal [Momordica charantia])

HSP 1 Score: 1362.8 bits (3526), Expect = 0.0e+00
Identity = 659/725 (90.90%), Postives = 695/725 (95.86%), Query Frame = 0

Query: 220 MDYRTLDSITLTDIEALGIASGVAEKLHKLLTEIIHSYGNGTPETWRHISKRVLSPDLPF 279
           MDY+TLDSIT+ DIEALG+AS VAEKLH LL+EII S+GNGTPETWRHISKRVLSPDLPF
Sbjct: 1   MDYKTLDSITVIDIEALGVASEVAEKLHGLLSEIIRSHGNGTPETWRHISKRVLSPDLPF 60

Query: 280 SFHQMMYYGCYKHYGLDPPAWMPEPENAVFTNVGQLLERRGKEFLGSNYRDPLSSFSSFQ 339
           SFHQMMYYGCYKHYG DPPAW+PEPENAVFTNVGQLL+RRGKEFLGSNYRDPLSSFSSFQ
Sbjct: 61  SFHQMMYYGCYKHYGPDPPAWIPEPENAVFTNVGQLLKRRGKEFLGSNYRDPLSSFSSFQ 120

Query: 340 EFSVSNPEVYWRTVLDEMHITFSKPPKCILQMNGSPESQFPNPGGQWLPGGVFNPAKDCL 399
           EFSVSNPEVYWRT+LDEMHITFSKPP CILQMN S ESQF +PGGQWLPG VFNPAKDCL
Sbjct: 121 EFSVSNPEVYWRTMLDEMHITFSKPPHCILQMNDSTESQFSSPGGQWLPGAVFNPAKDCL 180

Query: 400 SVNENRSLDDIAIIWRDEGCDNLPVKRLTLRELRAEVWLIAHALNSIGFEKGTAIAIDMP 459
           S+NENRSLDD+AIIWRDEGCDNLPVKRLTL ELR +VWLIAHALNSIGFEKGTAIAIDMP
Sbjct: 181 SLNENRSLDDVAIIWRDEGCDNLPVKRLTLGELRTDVWLIAHALNSIGFEKGTAIAIDMP 240

Query: 460 MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRVNISKAKAIFTQDLIIRGDKSIPLYS 519
           MNVNAVVIYL IVLAGHVVVSIADSF+AREISTR++ISKAKAIFTQDLIIRGDKSIPLYS
Sbjct: 241 MNVNAVVIYLGIVLAGHVVVSIADSFSAREISTRLDISKAKAIFTQDLIIRGDKSIPLYS 300

Query: 520 RVVDAQSLLAIVIPTRSTGFSMKLRDGDISWHAFLERVKDLKGVEFSAVEQSAESFTNIL 579
           RVVDAQS +AIVIP+RSTGFS KLRD DISWHAFLERV+DL+GVEF+AVEQ+AESFTNIL
Sbjct: 301 RVVDAQSPMAIVIPSRSTGFSRKLRDEDISWHAFLERVEDLRGVEFAAVEQAAESFTNIL 360

Query: 580 FSSGTTGEPKAIPWTIVTPLKAAADAWCHMDIHKGDVVAWPTNLGWMMGPWLVYASLLNS 639
           FSSGTTGEPKAIPWT+VTPLKAAADAWC+MDIHKGDVVAWPTNLGWMMGPWLVYASLLNS
Sbjct: 361 FSSGTTGEPKAIPWTLVTPLKAAADAWCYMDIHKGDVVAWPTNLGWMMGPWLVYASLLNS 420

Query: 640 ASLALYNGSPLGSGFVKFVQDAKITMLGVVPSIVRTWKITNCTSGYDWSSIRCFSSTGEA 699
           AS+ALYNGSPLGSGFVKFVQDAK+TMLGV+PSIVR+WK TNCTSGYDWSSIRCF+STGEA
Sbjct: 421 ASMALYNGSPLGSGFVKFVQDAKVTMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGEA 480

Query: 700 STVDECLWLMGRAGYKPVIEICGGTEIGGGFVAGSLLQPQALSAFSTPGMGCSLFILGND 759
           S VDE LWLMGRA YKPVIEICGGTEIGGGF+ GSLLQPQAL+AFSTP MGCSLFILGND
Sbjct: 481 SNVDENLWLMGRACYKPVIEICGGTEIGGGFITGSLLQPQALAAFSTPAMGCSLFILGND 540

Query: 760 GFPIPQHMPGIGELALGPFLFGASSTLLNADHYDIYFKGMPRWNGMVLRRHGDVFERSPR 819
           GFPIPQ+MPGIGELALGPFLFGASSTLLNADHYDIYFKGMP WNGMVLRRHGDVFERSPR
Sbjct: 541 GFPIPQNMPGIGELALGPFLFGASSTLLNADHYDIYFKGMPHWNGMVLRRHGDVFERSPR 600

Query: 820 GYYRAHGRADDTMNLGGIKVSSVEIERVCNTVDDSILETAAIGVPPHGGGPEQLVIAIVF 879
           GYYRAHGRADD MNLGGIKVSSVEIER+CNT+DDSILETAAIGVPP GGGPEQLVIA+V 
Sbjct: 601 GYYRAHGRADDAMNLGGIKVSSVEIERICNTIDDSILETAAIGVPPLGGGPEQLVIAVVL 660

Query: 880 KNPNETSPDLNKLKLSFNSSLQKNLNPLFRVHRVVPYPSLPRTATNKVMRRILRQQLALE 939
           KNP ETSPDL+KLKL FNSSLQKNLNPLFRVHRVVPYPSLPRTATNKVMRRILRQQLA+E
Sbjct: 661 KNPGETSPDLDKLKLCFNSSLQKNLNPLFRVHRVVPYPSLPRTATNKVMRRILRQQLAVE 720

Query: 940 HRPKL 945
            R KL
Sbjct: 721 RRTKL 725

BLAST of Spg022103 vs. NCBI nr
Match: XP_023529559.1 (probable acyl-activating enzyme 17, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1362.4 bits (3525), Expect = 0.0e+00
Identity = 660/725 (91.03%), Postives = 692/725 (95.45%), Query Frame = 0

Query: 220 MDYRTLDSITLTDIEALGIASGVAEKLHKLLTEIIHSYGNGTPETWRHISKRVLSPDLPF 279
           MDYR LDSIT+TDI+ALGIAS VA++LH LLT+II SYGNG+PETW HISKRVLSPDLPF
Sbjct: 1   MDYRALDSITVTDIKALGIASEVADELHGLLTDIIRSYGNGSPETWCHISKRVLSPDLPF 60

Query: 280 SFHQMMYYGCYKHYGLDPPAWMPEPENAVFTNVGQLLERRGKEFLGSNYRDPLSSFSSFQ 339
           SFHQMMYYGCYKHYG DPPAWMPE E+AVFTNVGQLLERRGKEFLGSNYRDP++SFSSFQ
Sbjct: 61  SFHQMMYYGCYKHYGSDPPAWMPELESAVFTNVGQLLERRGKEFLGSNYRDPITSFSSFQ 120

Query: 340 EFSVSNPEVYWRTVLDEMHITFSKPPKCILQMNGSPESQFPNPGGQWLPGGVFNPAKDCL 399
           EFSVS+PEVYW+ VLD+MHITFSKPPKCILQMN SP+ Q P PGGQWLPG VFNPAKDCL
Sbjct: 121 EFSVSSPEVYWKAVLDDMHITFSKPPKCILQMNDSPDCQSPCPGGQWLPGAVFNPAKDCL 180

Query: 400 SVNENRSLDDIAIIWRDEGCDNLPVKRLTLRELRAEVWLIAHALNSIGFEKGTAIAIDMP 459
           S  ENRSLDD AIIWR EGCDN+PVKRLTLRELR E WL+AHALNS+GFEKGTAIAIDMP
Sbjct: 181 SSKENRSLDDAAIIWRVEGCDNMPVKRLTLRELRTEAWLVAHALNSLGFEKGTAIAIDMP 240

Query: 460 MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRVNISKAKAIFTQDLIIRGDKSIPLYS 519
           MNV +VVIYLAIVLAGHVVVSIADSFAAREISTRVNISKAKAIFTQDLIIRGDKSIPLYS
Sbjct: 241 MNVTSVVIYLAIVLAGHVVVSIADSFAAREISTRVNISKAKAIFTQDLIIRGDKSIPLYS 300

Query: 520 RVVDAQSLLAIVIPTRSTGFSMKLRDGDISWHAFLERVKDLKGVEFSAVEQSAESFTNIL 579
           RVVDAQS LAIVIPTRSTG SMKLR GDISWHAFLERVKDLKGVEF+AVEQSA+SFTNIL
Sbjct: 301 RVVDAQSPLAIVIPTRSTGLSMKLRAGDISWHAFLERVKDLKGVEFAAVEQSAKSFTNIL 360

Query: 580 FSSGTTGEPKAIPWTIVTPLKAAADAWCHMDIHKGDVVAWPTNLGWMMGPWLVYASLLNS 639
           FSSGTTGEPKAIPWT+VTPLKAAADAWCHMDIHKGDVVAWPTNLGWMMGPWLVYASLLN 
Sbjct: 361 FSSGTTGEPKAIPWTLVTPLKAAADAWCHMDIHKGDVVAWPTNLGWMMGPWLVYASLLNK 420

Query: 640 ASLALYNGSPLGSGFVKFVQDAKITMLGVVPSIVRTWKITNCTSGYDWSSIRCFSSTGEA 699
           ASLALY+GSPLGSGFVKFVQDAK+TMLGVVPSIVR+WK TNCTSGYDWSSIRCFSSTGEA
Sbjct: 421 ASLALYDGSPLGSGFVKFVQDAKVTMLGVVPSIVRSWKSTNCTSGYDWSSIRCFSSTGEA 480

Query: 700 STVDECLWLMGRAGYKPVIEICGGTEIGGGFVAGSLLQPQALSAFSTPGMGCSLFILGND 759
           STVDECLWLMGRAGYKPVIEICGGTEIGGGFVAGSLLQ QALSAFSTPGMGCSLFILGND
Sbjct: 481 STVDECLWLMGRAGYKPVIEICGGTEIGGGFVAGSLLQAQALSAFSTPGMGCSLFILGND 540

Query: 760 GFPIPQHMPGIGELALGPFLFGASSTLLNADHYDIYFKGMPRWNGMVLRRHGDVFERSPR 819
           GFP+PQ+MPGIGELALGPFLFGASS LLNADHYD+YFKGMPRWNGMVLRRHGDVFERSPR
Sbjct: 541 GFPLPQNMPGIGELALGPFLFGASSALLNADHYDVYFKGMPRWNGMVLRRHGDVFERSPR 600

Query: 820 GYYRAHGRADDTMNLGGIKVSSVEIERVCNTVDDSILETAAIGVPPHGGGPEQLVIAIVF 879
           GYYRAHGRADDTMNLGGIKVSSVEIER+CN+VD+SILETAAIGVPPHGGGPEQL IAIVF
Sbjct: 601 GYYRAHGRADDTMNLGGIKVSSVEIERICNSVDESILETAAIGVPPHGGGPEQLTIAIVF 660

Query: 880 KNPNETSPDLNKLKLSFNSSLQKNLNPLFRVHRVVPYPSLPRTATNKVMRRILRQQLALE 939
           KN N ++PDLNKLKL FNSSLQKNLNPLF+VHRVVPYPSLPRTATNKVMRRILRQQLA E
Sbjct: 661 KNQNGSTPDLNKLKLCFNSSLQKNLNPLFKVHRVVPYPSLPRTATNKVMRRILRQQLAAE 720

Query: 940 HRPKL 945
           HR KL
Sbjct: 721 HRSKL 725

BLAST of Spg022103 vs. NCBI nr
Match: XP_022135245.1 (probable acyl-activating enzyme 17, peroxisomal [Momordica charantia])

HSP 1 Score: 1360.9 bits (3521), Expect = 0.0e+00
Identity = 652/725 (89.93%), Postives = 691/725 (95.31%), Query Frame = 0

Query: 220 MDYRTLDSITLTDIEALGIASGVAEKLHKLLTEIIHSYGNGTPETWRHISKRVLSPDLPF 279
           M+Y+ LDSIT  +IEALGIAS VAEKLH LL+EII SYGNGTPETWRHISKRVLSPDLPF
Sbjct: 1   MEYKALDSITAIEIEALGIASEVAEKLHGLLSEIIRSYGNGTPETWRHISKRVLSPDLPF 60

Query: 280 SFHQMMYYGCYKHYGLDPPAWMPEPENAVFTNVGQLLERRGKEFLGSNYRDPLSSFSSFQ 339
           SFHQMMYYGC+KHYG DPPAWMPEPENAVFTNVGQLLERRGKEFLGSNYRDP SSFSSFQ
Sbjct: 61  SFHQMMYYGCFKHYGPDPPAWMPEPENAVFTNVGQLLERRGKEFLGSNYRDPFSSFSSFQ 120

Query: 340 EFSVSNPEVYWRTVLDEMHITFSKPPKCILQMNGSPESQFPNPGGQWLPGGVFNPAKDCL 399
           EFSVSNPEVYWRTVLDEMHITFSKPP CILQMN SPESQF +PGGQWLPG VFNPAKDCL
Sbjct: 121 EFSVSNPEVYWRTVLDEMHITFSKPPNCILQMNDSPESQFRSPGGQWLPGAVFNPAKDCL 180

Query: 400 SVNENRSLDDIAIIWRDEGCDNLPVKRLTLRELRAEVWLIAHALNSIGFEKGTAIAIDMP 459
           S+NENRSLDD+AIIWRDEGCDNLPVKRLTLRELRAEVWL+AHALNSIGFEKGTAIAIDMP
Sbjct: 181 SINENRSLDDVAIIWRDEGCDNLPVKRLTLRELRAEVWLVAHALNSIGFEKGTAIAIDMP 240

Query: 460 MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRVNISKAKAIFTQDLIIRGDKSIPLYS 519
           MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTR+NISKAKAIFTQDLIIRGDKSIPLYS
Sbjct: 241 MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRLNISKAKAIFTQDLIIRGDKSIPLYS 300

Query: 520 RVVDAQSLLAIVIPTRSTGFSMKLRDGDISWHAFLERVKDLKGVEFSAVEQSAESFTNIL 579
           R++DAQS LAIVIPT STGFSMKLRD DISWHAFLERVKDLKGVEFSAVE+S ESFTNIL
Sbjct: 301 RIIDAQSPLAIVIPTNSTGFSMKLRDSDISWHAFLERVKDLKGVEFSAVEKSGESFTNIL 360

Query: 580 FSSGTTGEPKAIPWTIVTPLKAAADAWCHMDIHKGDVVAWPTNLGWMMGPWLVYASLLNS 639
           FSSGTTGEPKAIPWT+VTPLKAAADAWCHMDI  GDVVAWPTNLGWMMGPWLVYASLLN 
Sbjct: 361 FSSGTTGEPKAIPWTLVTPLKAAADAWCHMDIRHGDVVAWPTNLGWMMGPWLVYASLLNR 420

Query: 640 ASLALYNGSPLGSGFVKFVQDAKITMLGVVPSIVRTWKITNCTSGYDWSSIRCFSSTGEA 699
           AS+ALYNGSPL SGFVKFVQDAK+TMLGV+PSIVR+WK TNCTSGYDWSSIRCF+STGEA
Sbjct: 421 ASMALYNGSPLSSGFVKFVQDAKVTMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGEA 480

Query: 700 STVDECLWLMGRAGYKPVIEICGGTEIGGGFVAGSLLQPQALSAFSTPGMGCSLFILGND 759
           S++DE LWLMGRA YKP+IE CGGTEIGGGFV GSLLQ QAL+AFS P MGCSLFILGND
Sbjct: 481 SSIDEYLWLMGRACYKPIIEYCGGTEIGGGFVTGSLLQAQALAAFSAPAMGCSLFILGND 540

Query: 760 GFPIPQHMPGIGELALGPFLFGASSTLLNADHYDIYFKGMPRWNGMVLRRHGDVFERSPR 819
             PIPQ MPGIGELALGPF+FGASS+LLNADHYD+YFKGMPRWNGM+LRRHGD+FERSPR
Sbjct: 541 DSPIPQSMPGIGELALGPFMFGASSSLLNADHYDVYFKGMPRWNGMILRRHGDLFERSPR 600

Query: 820 GYYRAHGRADDTMNLGGIKVSSVEIERVCNTVDDSILETAAIGVPPHGGGPEQLVIAIVF 879
            YYRAHGRADDTMNLGGIKVSSVEIER+CNTVD+SILETAAIGVPPHGGGPEQLV+ +VF
Sbjct: 601 AYYRAHGRADDTMNLGGIKVSSVEIERICNTVDESILETAAIGVPPHGGGPEQLVVTVVF 660

Query: 880 KNPNETSPDLNKLKLSFNSSLQKNLNPLFRVHRVVPYPSLPRTATNKVMRRILRQQLALE 939
           KNP++TSPDL+KLKLSFNS+LQKNLNPLFRVHRVVPYPSLPRTATNKVMRR+LRQQLA+E
Sbjct: 661 KNPSDTSPDLDKLKLSFNSALQKNLNPLFRVHRVVPYPSLPRTATNKVMRRVLRQQLAIE 720

Query: 940 HRPKL 945
           H+ KL
Sbjct: 721 HKTKL 725

BLAST of Spg022103 vs. ExPASy Swiss-Prot
Match: F4KBF3 (Probable acyl-activating enzyme 17, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE17 PE=2 SV=1)

HSP 1 Score: 1047.0 bits (2706), Expect = 1.3e-304
Identity = 502/718 (69.92%), Postives = 604/718 (84.12%), Query Frame = 0

Query: 220 MDYRTLDSITLTDIEALGIASGVAEKLHKLLTEIIHSYGNGT-PETWRHISKRVLSPDLP 279
           M Y++L+SIT +DIEALGI+  V+EKL + L +IIH  G+ T PETW  IS+R+L P+LP
Sbjct: 1   MAYKSLNSITKSDIEALGISGDVSEKLLRDLEDIIH--GSSTPPETWIQISRRILHPNLP 60

Query: 280 FSFHQMMYYGCYKHYGLDPPAWMPEPENAVFTNVGQLLERRGKEFLGSNYRDPLSSFSSF 339
           FSFHQMMYYGCYK +G D PAW+P+P+ A  TNVG+LLE+RGKEFLG NY++P+SSFSSF
Sbjct: 61  FSFHQMMYYGCYKDFGPDLPAWIPDPKVASLTNVGKLLEKRGKEFLGGNYKNPVSSFSSF 120

Query: 340 QEFSVSNPEVYWRTVLDEMHITFSKPPKCILQMNGSPESQFPNPGGQWLPGGVFNPAKDC 399
           QEFSVSNPEVYW+TVLDE++I FS PPKCIL+ + S +    NPGG+WLPG   NPA++C
Sbjct: 121 QEFSVSNPEVYWKTVLDELNILFSVPPKCILEKDTSGD----NPGGKWLPGAYLNPARNC 180

Query: 400 LSVNENRSLDDIAIIWRDEGCDNLPVKRLTLRELRAEVWLIAHALNSIGFEKGTAIAIDM 459
           L+    R LDDI I WRDEG D+LPV  +TL ELR++VWL AHAL+++G E+ +AIA+DM
Sbjct: 181 LTNGFKRRLDDIVIRWRDEGSDDLPVNTMTLLELRSQVWLAAHALSALGLEEESAIAVDM 240

Query: 460 PMNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRVNISKAKAIFTQDLIIRGDKSIPLY 519
           PMNV +V+IYLAIVLAGHVVVSIADSF+ REISTR+ ISKAKAIFTQD+IIRGDKSIPLY
Sbjct: 241 PMNVESVIIYLAIVLAGHVVVSIADSFSPREISTRLKISKAKAIFTQDVIIRGDKSIPLY 300

Query: 520 SRVVDAQSLLAIVIPTRSTGFSMKLRDGDISWHAFLERVKDLKGVEFSAVEQSAESFTNI 579
            RVVDA++ LAIV+P R +   MKLR+ D+SW+ FL   ++L+GVE+ A E+ A ++TNI
Sbjct: 301 RRVVDAEAPLAIVVPARGSSCRMKLREKDLSWNNFLGNARNLRGVEYVAAEKPAGAYTNI 360

Query: 580 LFSSGTTGEPKAIPWTIVTPLKAAADAWCHMDIHKGDVVAWPTNLGWMMGPWLVYASLLN 639
           LFSSGTTGEPKAIPWT ++PLK+AADAWCH+D+ +GDVVAWPTNLGWMMGPWLVYASL+N
Sbjct: 361 LFSSGTTGEPKAIPWTNISPLKSAADAWCHLDVQRGDVVAWPTNLGWMMGPWLVYASLIN 420

Query: 640 SASLALYNGSPLGSGFVKFVQDAKITMLGVVPSIVRTWKITNCTSGYDWSSIRCFSSTGE 699
            A + LYNGSPLG  F KFVQDA++++LGV+PSIVRTW+ +N TSGYDWS IRCF STGE
Sbjct: 421 GACMGLYNGSPLGPTFAKFVQDAEVSVLGVIPSIVRTWQNSNSTSGYDWSRIRCFGSTGE 480

Query: 700 ASTVDECLWLMGRAGYKPVIEICGGTEIGGGFVAGSLLQPQALSAFSTPGMGCSLFILGN 759
           AS +DE LWLMGRA YKPVIE CGGTEIGG F++GSLLQPQ+L+AFST  MGC LFIL  
Sbjct: 481 ASNIDEYLWLMGRAHYKPVIEYCGGTEIGGSFISGSLLQPQSLAAFSTAAMGCKLFILDE 540

Query: 760 DGFPIPQHMPGIGELALGPFLFGASSTLLNADHYDIYFKGMPRWNGMVLRRHGDVFERSP 819
           D  PIP +  G+GELAL P +FGASSTLLN +H+ +YF+GMP + G +LRRHGD+FER+ 
Sbjct: 541 DSNPIPPYAAGVGELALCPHMFGASSTLLNGNHFKVYFQGMPTFQGQILRRHGDLFERTS 600

Query: 820 RGYYRAHGRADDTMNLGGIKVSSVEIERVCNTVDDSILETAAIGVPPHGGGPEQLVIAIV 879
           +GYYRAHGRADDTMNLGGIKV S+EIERVCN+VDDS+LETAAIGVPP  GGPEQLVIA+V
Sbjct: 601 KGYYRAHGRADDTMNLGGIKVGSIEIERVCNSVDDSVLETAAIGVPPPSGGPEQLVIAVV 660

Query: 880 FKNPNETSPDLNKLKLSFNSSLQKNLNPLFRVHRVVPYPSLPRTATNKVMRRILRQQL 937
           FK+P  ++PDLN LK SFNS +QK LNPLF+V  VV  PSLPRTATNKVMRR+LRQQL
Sbjct: 661 FKSPEFSNPDLNLLKKSFNSEIQKKLNPLFKVSSVVTLPSLPRTATNKVMRRVLRQQL 712

BLAST of Spg022103 vs. ExPASy Swiss-Prot
Match: M4IRL9 (Probable CoA ligase CCL12 OS=Humulus lupulus OX=3486 GN=CCL12 PE=2 SV=1)

HSP 1 Score: 709.9 bits (1831), Expect = 3.8e-203
Identity = 359/721 (49.79%), Postives = 486/721 (67.41%), Query Frame = 0

Query: 228 ITLTDIEALGIASGVAEKLHKLLTEIIH-SYGNGTPETWRH-ISKRVLSPDLPFSFHQMM 287
           + + D+   G+++  A+   ++L +I++ + G    E WR  +++R+L P  P   HQ++
Sbjct: 4   VGVEDLVKAGLSTEEAKYFQRVLEDIVNGAKGLDPSEVWREVVARRLLKPSHPHELHQLL 63

Query: 288 YYGCYKHYGLD----PPAWMPEPENAVFTNVGQLLERRGKEFLGSNYRDPLSSFSSFQEF 347
           Y+  Y ++ +     P  W P    +  TN+G+L+E+ G E LG  Y+DP++SFS F++F
Sbjct: 64  YHSVYANWDVSTRGPPLYWFPSLHQSKKTNLGRLMEKYGSEVLGPLYKDPITSFSLFRKF 123

Query: 348 SVSNPEVYWRTVLDEMHITFSKPPKCILQMNGSPESQFPNPGGQWLPGGVFNPAKDCL-- 407
           SV  PE YW  VL E+ ++F + PKCIL       +      G  LPG V N A+ CL  
Sbjct: 124 SVQQPEAYWSIVLKELSVSFQEAPKCILD-----SADKSKRSGSLLPGSVLNIAECCLLP 183

Query: 408 SVNENRSLDDIAIIWRDEGCDNLPVKRLTLRELRAEVWLIAHALNSIGFEKGTAIAIDMP 467
           +    +  D  AI+WRDEG D+  V  LTL+ELR +V L+A+AL+++ F KG AIAIDMP
Sbjct: 184 TSYPMKRDDSPAIVWRDEGYDDSEVNVLTLKELREKVMLVANALDAM-FSKGDAIAIDMP 243

Query: 468 MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRVNISKAKAIFTQDLIIRGDKSIPLYS 527
           M VNAVVIYLAI+L+G VVVSIADSFAA+EI+TR+ +S AKAIFTQD I+RG +   LYS
Sbjct: 244 MTVNAVVIYLAIILSGFVVVSIADSFAAKEIATRLRVSNAKAIFTQDFILRGGRKFSLYS 303

Query: 528 RVVDAQSLLAIVIPTRSTGFSMKLRDGDISWHAFLERVKD-LKGVEFSAVEQSAESFTNI 587
           RVVDA    AIV+P   +   ++LR+ DISW  F+  V +  +   ++   Q  +S  NI
Sbjct: 304 RVVDAAPHKAIVLPVNGSNLGLQLREQDISWEKFISNVSNQARSNHYTPFYQPVDSLINI 363

Query: 588 LFSSGTTGEPKAIPWTIVTPLKAAADAWCHMDIHKGDVVAWPTNLGWMMGPWLVYASLLN 647
           LFSSGTTGEPKAIPWT ++PL+ AAD W H+D   GDV  WPTNLGW+MGP L+++S L+
Sbjct: 364 LFSSGTTGEPKAIPWTQLSPLRCAADTWAHIDAQVGDVFCWPTNLGWVMGPVLLFSSFLS 423

Query: 648 SASLALYNGSPLGSGFVKFVQDAKITMLGVVPSIVRTWKITNCTSGYDWSSIRCFSSTGE 707
            A+LALY+GSPLG GF KFVQD+ +T+LG VPS+V+ WK T C  G DW+ I+ F+STGE
Sbjct: 424 GATLALYHGSPLGHGFGKFVQDSGVTILGTVPSLVKAWKNTQCMKGLDWTKIKVFASTGE 483

Query: 708 ASTVDECLWLMGRAGYKPVIEICGGTEIGGGFVAGSLLQPQALSAFSTPGMGCSLFILGN 767
           AS VD+ LWL  +A Y+P+IE CGGTE+   ++ GSL+QPQA  AFST  M   L IL  
Sbjct: 484 ASNVDDDLWLSSQAYYQPIIECCGGTELASSYIQGSLVQPQAFGAFSTAAMTVGLVILDE 543

Query: 768 DGFPIPQHMPGIGELALGPFLFGASSTLLNADHYDIYFKGMPRWNGMVLRRHGDVFERSP 827
           +G P P      GE+ L P   GA+  LLNAD+  +YFKGMP +NGM LRRHGD+ +R+ 
Sbjct: 544 NGIPYPDKQECTGEVGLFPLYLGATDRLLNADNEKVYFKGMPLYNGMSLRRHGDILKRTA 603

Query: 828 RGYYRAHGRADDTMNLGGIKVSSVEIERVCNTVDDSILETAAIGVPPHGGGPEQLVIAIV 887
            G++  HGRADDTMNLGGIK SS+EIERVCN  D+S++ET A+ V P  GGPEQLV+ +V
Sbjct: 604 GGFFIVHGRADDTMNLGGIKTSSIEIERVCNRADESVMETTAVSVAPVSGGPEQLVMFVV 663

Query: 888 FKNPNETSPDLNKLKLSFNSSLQKNLNPLFRVHRVVPYPSLPRTATNKVMRRILRQQLAL 940
            K+ +    +  +LK  F+ ++Q NLNPLF+V  V      PRTA+NK++RR+LR Q+  
Sbjct: 664 LKSGHNN--EAERLKKKFSKAIQSNLNPLFKVSFVKIVEEFPRTASNKLLRRVLRDQMKH 716

BLAST of Spg022103 vs. ExPASy Swiss-Prot
Match: Q84P17 (Probable acyl-activating enzyme 18, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE18 PE=2 SV=1)

HSP 1 Score: 696.8 bits (1797), Expect = 3.3e-199
Identity = 352/728 (48.35%), Postives = 477/728 (65.52%), Query Frame = 0

Query: 222 YRTLDSITLTDIEALGIASGVAEKLHKLLTEIIHSYGNGTP-ETWRH-ISKRVLSPDLPF 281
           ++++  ++  D    G+    A++  KL++++I       P + W+  + + VL P  P 
Sbjct: 2   WKSIGELSCDDYVKAGLTLEDAKEFDKLVSDVITKAIETDPRDQWKALVDESVLKPWHPH 61

Query: 282 SFHQMMYYGCYKHYGLD----PPAWMPEPENAVFTNVGQLLERRGKEFLGSNYRDPLSSF 341
             HQ++YY  Y ++       P  W P    +  TN+G+L+E  G   LG +Y++PL SF
Sbjct: 62  PLHQLLYYSVYSNWDSSVHGPPLYWFPSLSQSKSTNLGKLMEYHGPRLLGPSYKNPLESF 121

Query: 342 SSFQEFSVSNPEVYWRTVLDEMHITFSKPPKCILQMNGSPESQFPNPGGQWLPGGVFNPA 401
             F+ FSV +PEVYW  V+DE+ + F  PP+CIL  +         P G WLP  V N A
Sbjct: 122 ELFRRFSVEHPEVYWSFVIDELSLVFHTPPRCILNKS--------KPEGTWLPDAVLNIA 181

Query: 402 KDCL--SVNENRSLDDIAIIWRDEGCDNLPVKRLTLRELRAEVWLIAHALNSIGFEKGTA 461
           + CL  S +  +  D +A++WR+EG D+ PV R+T++ELR +V L+A+A++   FEKG  
Sbjct: 182 ECCLMPSSHPKKEDDSVAVVWRNEGFDDSPVNRMTIKELREQVMLVANAISG-SFEKGDT 241

Query: 462 IAIDMPMNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRVNISKAKAIFTQDLIIRGDK 521
           IAIDMPM V+AV+IYLAI+LAG +VVSIADSFAA+EI+TR+ ISKAK IFTQD I+RG +
Sbjct: 242 IAIDMPMTVDAVIIYLAIILAGCIVVSIADSFAAKEIATRLKISKAKGIFTQDYILRGGR 301

Query: 522 SIPLYSRVVDAQSLLAIVIPTRSTGFSMKLRDGDISWHAFLERVKDLKGVE--FSAVEQS 581
             PLYSRVV+A     IV+P   T   ++LR+ D+SW  FL   K     E  +  +   
Sbjct: 302 RFPLYSRVVEAAPSKVIVLPASGTELHVQLREQDVSWMDFLSNAKPHSSGENYYRPIYLP 361

Query: 582 AESFTNILFSSGTTGEPKAIPWTIVTPLKAAADAWCHMDIHKGDVVAWPTNLGWMMGPWL 641
            ES  NILFSSGTTGEPKAIPWT ++P+++A D W H+D+  G    WPTNLGW+MGP L
Sbjct: 362 VESVINILFSSGTTGEPKAIPWTQLSPIRSACDGWAHLDVQVGHTYCWPTNLGWVMGPTL 421

Query: 642 VYASLLNSASLALYNGSPLGSGFVKFVQDAKITMLGVVPSIVRTWKITNCTSGYDWSSIR 701
           +++  L  A+LALY+GSPLG GF KFVQDA +T+LG VPS+V+TWK TNC  G +W+ I+
Sbjct: 422 MFSCFLTGATLALYSGSPLGRGFGKFVQDAGVTVLGTVPSLVKTWKRTNCMEGLNWTKIK 481

Query: 702 CFSSTGEASTVDECLWLMGRAGYKPVIEICGGTEIGGGFVAGSLLQPQALSAFSTPGMGC 761
            F++TGEAS VD+ LWL  +A YKPVIE CGGTE+   ++ GS LQPQA  AFSTP M  
Sbjct: 482 FFATTGEASNVDDVLWLSSKADYKPVIECCGGTELASSYIIGSPLQPQAFGAFSTPSMTT 541

Query: 762 SLFILGNDGFPIPQHMPGIGELALGPFLFGASSTLLNADHYDIYFKGMPRWNGMVLRRHG 821
            + I   +G P P   P  GE+ L P   GA+  LLNA+H ++YFKGMP +    LRRHG
Sbjct: 542 RIIIFDENGVPYPDDQPCTGEVGLFPQHLGATDRLLNANHDEVYFKGMPMYKETRLRRHG 601

Query: 822 DVFERSPRGYYRAHGRADDTMNLGGIKVSSVEIERVCNTVDDSILETAAIGVPPHGGGPE 881
           D+ +R+  GYY   GRADDTMNLGGIK SS+EIERVC+  D+ I ETAA+ + P  GGPE
Sbjct: 602 DIVKRTVGGYYNVQGRADDTMNLGGIKTSSIEIERVCDQADECISETAAVTLTPPNGGPE 661

Query: 882 QLVIAIVFKNPNETSPDLNKLKLSFNSSLQKNLNPLFRVHRVVPYPSLPRTATNKVMRRI 940
            LVI  V K   +      +LK+ F+ ++QK+LNPLF+V  V   P  PRTA++K++RR+
Sbjct: 662 LLVIFAVLKEGFKQQSG-EELKMKFSRTIQKDLNPLFKVSFVKIVPEFPRTASSKLLRRV 719

BLAST of Spg022103 vs. ExPASy Swiss-Prot
Match: P27550 (Acetyl-coenzyme A synthetase OS=Escherichia coli (strain K12) OX=83333 GN=acs PE=1 SV=2)

HSP 1 Score: 186.0 bits (471), Expect = 1.9e-45
Identity = 172/620 (27.74%), Postives = 277/620 (44.68%), Query Frame = 0

Query: 335 FSSFQEFSVSNPEVYW---RTVLDEMHITFSKPPKCILQMNGSPESQFPNPGGQWLPGGV 394
           + +  + S++ P+ +W     +LD     + KP + +   + +P     N   +W   G 
Sbjct: 24  YEAMYQQSINVPDTFWGEQGKILD-----WIKPYQKVKNTSFAP----GNVSIKWYEDGT 83

Query: 395 FNPAKDCLSVNENRSLDDIAIIWRDEGCDNLPVKRLTLRELRAEVWLIAHALNSIGFEKG 454
            N A +CL  +   + D  AIIW  EG D    K ++ +EL  +V   A+ L  +G +KG
Sbjct: 84  LNLAANCLDRHLQENGDRTAIIW--EGDDASQSKHISYKELHRDVCRFANTLLELGIKKG 143

Query: 455 TAIAIDMPMNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRVNISKAKAIFTQDLIIRG 514
             +AI MPM   A V  LA    G V   I   F+   ++ R+  S ++ + T D  +R 
Sbjct: 144 DVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPEAVAGRIIDSNSRLVITSDEGVRA 203

Query: 515 DKSIPLYSRVVDA-----QSLLAIVIPTRSTGFSMKLRDG-DISWHAFLERVKDLKGVEF 574
            +SIPL   V DA      + +  V+  + TG  +  ++G D+ WH  +E+  D    + 
Sbjct: 204 GRSIPLKKNVDDALKNPNVTSVEHVVVLKRTGGKIDWQEGRDLWWHDLVEQASD----QH 263

Query: 575 SAVEQSAESFTNILFSSGTTGEPKAIPWTIVTPLKAAADAWCHM-DIHKGDVVAWPTNLG 634
            A E +AE    IL++SG+TG+PK +  T    L  AA  + ++ D H GD+     ++G
Sbjct: 264 QAEEMNAEDPLFILYTSGSTGKPKGVLHTTGGYLVYAALTFKYVFDYHPGDIYWCTADVG 323

Query: 635 WMMG-PWLVYASLLNSASLALYNGS---PLGSGFVKFVQDAKITMLGVVPSIVRTWKI-- 694
           W+ G  +L+Y  L   A+  ++ G    P  +   + V   ++ +L   P+ +R      
Sbjct: 324 WVTGHSYLLYGPLACGATTLMFEGVPNWPTPARMAQVVDKHQVNILYTAPTAIRALMAEG 383

Query: 695 TNCTSGYDWSSIRCFSSTGEASTVDECLWLMGRAGYK--PVIEICGGTEIGGGFVAGSLL 754
                G D SS+R   S GE    +   W   + G +  PV++    TE  GGF+   L 
Sbjct: 384 DKAIEGTDRSSLRILGSVGEPINPEAWEWYWKKIGNEKCPVVDTWWQTET-GGFMITPLP 443

Query: 755 QPQALSAFST--PGMGCSLFILGNDGFPIPQHMPGIGELALGPFLFGASSTLLNADHYDI 814
               L A S   P  G    ++ N+G P+       G L +     G + TL   DH   
Sbjct: 444 GATELKAGSATRPFFGVQPALVDNEGNPLEGATE--GSLVITDSWPGQARTLF-GDHERF 503

Query: 815 YFKGMPRWNGMVLRRHGDVFERSPRGYYRAHGRADDTMNLGGIKVSSVEIERVCNTVDDS 874
                  +  M     GD   R   GYY   GR DD +N+ G ++ + EIE         
Sbjct: 504 EQTYFSTFKNMYF--SGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESAL-VAHPK 563

Query: 875 ILETAAIGVPPHGGGPEQLVIAIVFKNPNETSPDLNKLKLSFNSSLQKNLNPLFRVHRVV 934
           I E A +G+ PH    + +   +   +  E SP+   L     + ++K + PL     + 
Sbjct: 564 IAEAAVVGI-PHNIKGQAIYAYVTLNHGEEPSPE---LYAEVRNWVRKEIGPLATPDVLH 617

BLAST of Spg022103 vs. ExPASy Swiss-Prot
Match: A7MPJ7 (Acetyl-coenzyme A synthetase OS=Cronobacter sakazakii (strain ATCC BAA-894) OX=290339 GN=acs PE=3 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 5.6e-45
Identity = 173/613 (28.22%), Postives = 269/613 (43.88%), Query Frame = 0

Query: 342 SVSNPEVYW---RTVLDEMHITFSKPPKCILQMNGSPESQFPNPGGQWLPGGVFNPAKDC 401
           SV +P+ +W     +LD     +  P + +   + +P     N   +W   G  N A +C
Sbjct: 31  SVHDPDAFWGEQGKILD-----WITPYQTVKNTSFAP----GNVSIKWYEDGTLNLAANC 90

Query: 402 LSVNENRSLDDIAIIWRDEGCDNLPVKRLTLRELRAEVWLIAHALNSIGFEKGTAIAIDM 461
           L  + +   D  AIIW  EG D    K ++ REL  +V   A+ L S+G +KG  +AI M
Sbjct: 91  LDRHLSERGDQTAIIW--EGDDATQSKHISYRELHRDVCRFANTLTSLGIKKGDVVAIYM 150

Query: 462 PMNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRVNISKAKAIFTQDLIIRGDKSIPLY 521
           PM   A V  LA    G V   I   F+   ++ R+  S A+ + T D  +R  ++IPL 
Sbjct: 151 PMVPEAAVAMLACARIGAVHSVIFGGFSPEAVAGRIIDSSARLVITADEGVRAGRAIPLK 210

Query: 522 SRVVDAQSLLAI-----VIPTRSTGFSMKLRDG-DISWHAFLERVKDLKGVEFSAVEQSA 581
             V DA     +     V+  + TG +++  +G D+ W   +++           VE +A
Sbjct: 211 KNVDDALKNPGVTSVEHVVVFKRTGGNIEWHEGRDLWWSELVKKA----SAHHQPVEMNA 270

Query: 582 ESFTNILFSSGTTGEPKAIPWTIVTPLKAAADAWCH-MDIHKGDVVAWPTNLGWMMG-PW 641
           E    IL++SG+TG+PK +  T    L  AA  + +  D H GD+     ++GW+ G  +
Sbjct: 271 EDPLFILYTSGSTGKPKGVLHTTGGYLVYAATTFLYAFDYHPGDIYWCTADVGWVTGHSY 330

Query: 642 LVYASLLNSASLALYNGS---PLGSGFVKFVQDAKITMLGVVPSIVRTWKI--TNCTSGY 701
           L+Y  L   A+  ++ G    P  +   + V   K+ +L   P+ +R           G 
Sbjct: 331 LLYGPLACGATTLMFEGVPNWPSANRMAQVVDKHKVNILYTAPTAIRALMAEGDKAIDGT 390

Query: 702 DWSSIRCFSSTGEASTVDECLWLMGRAGYK--PVIEICGGTEIGGGFVAGSLLQPQALSA 761
           D SS+R   S GE    +   W   + G +  PVI+    TE  GGF+   L     L A
Sbjct: 391 DRSSLRILGSVGEPINPEAWEWYWKKIGNEKCPVIDTWWQTET-GGFMITPLPGATELKA 450

Query: 762 FST--PGMGCSLFILGNDGFPIPQHMPGIGELALGPFLFGASSTLLNADHYDIYFKGMPR 821
            S   P  G    ++ N+G   PQ     G L +     G + TL   DH          
Sbjct: 451 GSATRPFFGVQPALVDNEG--NPQQGATEGNLVITDSWPGQARTLF-GDHERFEQTYFST 510

Query: 822 WNGMVLRRHGDVFERSPRGYYRAHGRADDTMNLGGIKVSSVEIERVCNTVDDSILETAAI 881
           +  M     GD   R   GYY   GR DD +N+ G ++ + EIE         I E A +
Sbjct: 511 FKNMYF--SGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESAL-VSHPKIAEAAVV 570

Query: 882 GVPPHGGGPEQLVIAIVFKNPNETSPDLNKLKLSFNSSLQKNLNPLFRVHRVVPYPSLPR 935
           G+ PH    + +   +   +  E S +L        + ++K + PL     +    SLP+
Sbjct: 571 GI-PHSIKGQAIYAYVTLNHGEEPSAELYS---EVRNWVRKEIGPLATPDVLHWTDSLPK 617

BLAST of Spg022103 vs. ExPASy TrEMBL
Match: A0A6J1EU22 (probable acyl-activating enzyme 17, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111437755 PE=4 SV=1)

HSP 1 Score: 1365.9 bits (3534), Expect = 0.0e+00
Identity = 659/725 (90.90%), Postives = 695/725 (95.86%), Query Frame = 0

Query: 220 MDYRTLDSITLTDIEALGIASGVAEKLHKLLTEIIHSYGNGTPETWRHISKRVLSPDLPF 279
           MDYR LDSIT+TDI+ALGIAS VA++LH +LT+II SYGNG+PETW HISKRVLSPDLPF
Sbjct: 1   MDYRALDSITVTDIKALGIASEVADELHGILTDIIRSYGNGSPETWCHISKRVLSPDLPF 60

Query: 280 SFHQMMYYGCYKHYGLDPPAWMPEPENAVFTNVGQLLERRGKEFLGSNYRDPLSSFSSFQ 339
           SFHQMMYYGCYKHYG DPPAWMPE E+AVFTNVGQLLERRGKEFLGSNYRDP++SFSSFQ
Sbjct: 61  SFHQMMYYGCYKHYGSDPPAWMPELESAVFTNVGQLLERRGKEFLGSNYRDPITSFSSFQ 120

Query: 340 EFSVSNPEVYWRTVLDEMHITFSKPPKCILQMNGSPESQFPNPGGQWLPGGVFNPAKDCL 399
           EFSVSNPEVYW+ VLD+MHITFSKPPKCILQMN SP+ Q P PGGQWLPG VFNPAKDCL
Sbjct: 121 EFSVSNPEVYWKAVLDDMHITFSKPPKCILQMNDSPDCQSPCPGGQWLPGAVFNPAKDCL 180

Query: 400 SVNENRSLDDIAIIWRDEGCDNLPVKRLTLRELRAEVWLIAHALNSIGFEKGTAIAIDMP 459
           S+NENRSLDD A+IWRDEGCDN+PVKRLTLRELR E WL+AHALNS+GFEKGTAIAIDMP
Sbjct: 181 SLNENRSLDDAAVIWRDEGCDNMPVKRLTLRELRTEAWLVAHALNSLGFEKGTAIAIDMP 240

Query: 460 MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRVNISKAKAIFTQDLIIRGDKSIPLYS 519
           MNV +VVIYLAIVLAGHVVVSIADSFAAREISTRVNISKAKAIFTQDLIIRGDKSI LYS
Sbjct: 241 MNVTSVVIYLAIVLAGHVVVSIADSFAAREISTRVNISKAKAIFTQDLIIRGDKSIHLYS 300

Query: 520 RVVDAQSLLAIVIPTRSTGFSMKLRDGDISWHAFLERVKDLKGVEFSAVEQSAESFTNIL 579
           RVVDAQS LAIVIPTRSTG SMKLRDGDISWHAFLERVKDLKGVEF+AVEQSAESFTNIL
Sbjct: 301 RVVDAQSPLAIVIPTRSTGLSMKLRDGDISWHAFLERVKDLKGVEFAAVEQSAESFTNIL 360

Query: 580 FSSGTTGEPKAIPWTIVTPLKAAADAWCHMDIHKGDVVAWPTNLGWMMGPWLVYASLLNS 639
           FSSGTTGEPKAIPWT+VTPLKAAADAWCHMDIHKGDVVAWPTNLGWMMGPWLVYASLLN 
Sbjct: 361 FSSGTTGEPKAIPWTLVTPLKAAADAWCHMDIHKGDVVAWPTNLGWMMGPWLVYASLLNK 420

Query: 640 ASLALYNGSPLGSGFVKFVQDAKITMLGVVPSIVRTWKITNCTSGYDWSSIRCFSSTGEA 699
           ASLALY+GSPLGSGFVKFVQDAK+TMLGVVPSIVR+WK TNCTSGYDWSSIRCFSSTGEA
Sbjct: 421 ASLALYDGSPLGSGFVKFVQDAKVTMLGVVPSIVRSWKSTNCTSGYDWSSIRCFSSTGEA 480

Query: 700 STVDECLWLMGRAGYKPVIEICGGTEIGGGFVAGSLLQPQALSAFSTPGMGCSLFILGND 759
           STVDECLWLMGRAGYKPVIEICGGTEIGGGFVAGSLLQ QALSAFSTPGMGCSLFILGND
Sbjct: 481 STVDECLWLMGRAGYKPVIEICGGTEIGGGFVAGSLLQAQALSAFSTPGMGCSLFILGND 540

Query: 760 GFPIPQHMPGIGELALGPFLFGASSTLLNADHYDIYFKGMPRWNGMVLRRHGDVFERSPR 819
           GFP+PQ+MPGIGELALGPFLFGASSTLLN+DHYD+YFKGMPRWNGMVLRRHGDVFERSPR
Sbjct: 541 GFPLPQNMPGIGELALGPFLFGASSTLLNSDHYDVYFKGMPRWNGMVLRRHGDVFERSPR 600

Query: 820 GYYRAHGRADDTMNLGGIKVSSVEIERVCNTVDDSILETAAIGVPPHGGGPEQLVIAIVF 879
           GYYRAHGRADDTMNLGGIKVSSVEIER+CN+VD+SILETAAIGVPPHGGGPEQL IAIV 
Sbjct: 601 GYYRAHGRADDTMNLGGIKVSSVEIERICNSVDESILETAAIGVPPHGGGPEQLTIAIVS 660

Query: 880 KNPNETSPDLNKLKLSFNSSLQKNLNPLFRVHRVVPYPSLPRTATNKVMRRILRQQLALE 939
           KN + ++PDLNKLKL FNSSLQKNLNPLF+VHRVVPYPSLPRTATNKVMRRILRQQLA +
Sbjct: 661 KNQSGSTPDLNKLKLCFNSSLQKNLNPLFKVHRVVPYPSLPRTATNKVMRRILRQQLAAQ 720

Query: 940 HRPKL 945
           HR KL
Sbjct: 721 HRSKL 725

BLAST of Spg022103 vs. ExPASy TrEMBL
Match: A0A6J1C048 (probable acyl-activating enzyme 17, peroxisomal OS=Momordica charantia OX=3673 GN=LOC111007256 PE=4 SV=1)

HSP 1 Score: 1362.8 bits (3526), Expect = 0.0e+00
Identity = 659/725 (90.90%), Postives = 695/725 (95.86%), Query Frame = 0

Query: 220 MDYRTLDSITLTDIEALGIASGVAEKLHKLLTEIIHSYGNGTPETWRHISKRVLSPDLPF 279
           MDY+TLDSIT+ DIEALG+AS VAEKLH LL+EII S+GNGTPETWRHISKRVLSPDLPF
Sbjct: 1   MDYKTLDSITVIDIEALGVASEVAEKLHGLLSEIIRSHGNGTPETWRHISKRVLSPDLPF 60

Query: 280 SFHQMMYYGCYKHYGLDPPAWMPEPENAVFTNVGQLLERRGKEFLGSNYRDPLSSFSSFQ 339
           SFHQMMYYGCYKHYG DPPAW+PEPENAVFTNVGQLL+RRGKEFLGSNYRDPLSSFSSFQ
Sbjct: 61  SFHQMMYYGCYKHYGPDPPAWIPEPENAVFTNVGQLLKRRGKEFLGSNYRDPLSSFSSFQ 120

Query: 340 EFSVSNPEVYWRTVLDEMHITFSKPPKCILQMNGSPESQFPNPGGQWLPGGVFNPAKDCL 399
           EFSVSNPEVYWRT+LDEMHITFSKPP CILQMN S ESQF +PGGQWLPG VFNPAKDCL
Sbjct: 121 EFSVSNPEVYWRTMLDEMHITFSKPPHCILQMNDSTESQFSSPGGQWLPGAVFNPAKDCL 180

Query: 400 SVNENRSLDDIAIIWRDEGCDNLPVKRLTLRELRAEVWLIAHALNSIGFEKGTAIAIDMP 459
           S+NENRSLDD+AIIWRDEGCDNLPVKRLTL ELR +VWLIAHALNSIGFEKGTAIAIDMP
Sbjct: 181 SLNENRSLDDVAIIWRDEGCDNLPVKRLTLGELRTDVWLIAHALNSIGFEKGTAIAIDMP 240

Query: 460 MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRVNISKAKAIFTQDLIIRGDKSIPLYS 519
           MNVNAVVIYL IVLAGHVVVSIADSF+AREISTR++ISKAKAIFTQDLIIRGDKSIPLYS
Sbjct: 241 MNVNAVVIYLGIVLAGHVVVSIADSFSAREISTRLDISKAKAIFTQDLIIRGDKSIPLYS 300

Query: 520 RVVDAQSLLAIVIPTRSTGFSMKLRDGDISWHAFLERVKDLKGVEFSAVEQSAESFTNIL 579
           RVVDAQS +AIVIP+RSTGFS KLRD DISWHAFLERV+DL+GVEF+AVEQ+AESFTNIL
Sbjct: 301 RVVDAQSPMAIVIPSRSTGFSRKLRDEDISWHAFLERVEDLRGVEFAAVEQAAESFTNIL 360

Query: 580 FSSGTTGEPKAIPWTIVTPLKAAADAWCHMDIHKGDVVAWPTNLGWMMGPWLVYASLLNS 639
           FSSGTTGEPKAIPWT+VTPLKAAADAWC+MDIHKGDVVAWPTNLGWMMGPWLVYASLLNS
Sbjct: 361 FSSGTTGEPKAIPWTLVTPLKAAADAWCYMDIHKGDVVAWPTNLGWMMGPWLVYASLLNS 420

Query: 640 ASLALYNGSPLGSGFVKFVQDAKITMLGVVPSIVRTWKITNCTSGYDWSSIRCFSSTGEA 699
           AS+ALYNGSPLGSGFVKFVQDAK+TMLGV+PSIVR+WK TNCTSGYDWSSIRCF+STGEA
Sbjct: 421 ASMALYNGSPLGSGFVKFVQDAKVTMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGEA 480

Query: 700 STVDECLWLMGRAGYKPVIEICGGTEIGGGFVAGSLLQPQALSAFSTPGMGCSLFILGND 759
           S VDE LWLMGRA YKPVIEICGGTEIGGGF+ GSLLQPQAL+AFSTP MGCSLFILGND
Sbjct: 481 SNVDENLWLMGRACYKPVIEICGGTEIGGGFITGSLLQPQALAAFSTPAMGCSLFILGND 540

Query: 760 GFPIPQHMPGIGELALGPFLFGASSTLLNADHYDIYFKGMPRWNGMVLRRHGDVFERSPR 819
           GFPIPQ+MPGIGELALGPFLFGASSTLLNADHYDIYFKGMP WNGMVLRRHGDVFERSPR
Sbjct: 541 GFPIPQNMPGIGELALGPFLFGASSTLLNADHYDIYFKGMPHWNGMVLRRHGDVFERSPR 600

Query: 820 GYYRAHGRADDTMNLGGIKVSSVEIERVCNTVDDSILETAAIGVPPHGGGPEQLVIAIVF 879
           GYYRAHGRADD MNLGGIKVSSVEIER+CNT+DDSILETAAIGVPP GGGPEQLVIA+V 
Sbjct: 601 GYYRAHGRADDAMNLGGIKVSSVEIERICNTIDDSILETAAIGVPPLGGGPEQLVIAVVL 660

Query: 880 KNPNETSPDLNKLKLSFNSSLQKNLNPLFRVHRVVPYPSLPRTATNKVMRRILRQQLALE 939
           KNP ETSPDL+KLKL FNSSLQKNLNPLFRVHRVVPYPSLPRTATNKVMRRILRQQLA+E
Sbjct: 661 KNPGETSPDLDKLKLCFNSSLQKNLNPLFRVHRVVPYPSLPRTATNKVMRRILRQQLAVE 720

Query: 940 HRPKL 945
            R KL
Sbjct: 721 RRTKL 725

BLAST of Spg022103 vs. ExPASy TrEMBL
Match: A0A6J1C0M0 (probable acyl-activating enzyme 17, peroxisomal OS=Momordica charantia OX=3673 GN=LOC111007255 PE=4 SV=1)

HSP 1 Score: 1360.9 bits (3521), Expect = 0.0e+00
Identity = 652/725 (89.93%), Postives = 691/725 (95.31%), Query Frame = 0

Query: 220 MDYRTLDSITLTDIEALGIASGVAEKLHKLLTEIIHSYGNGTPETWRHISKRVLSPDLPF 279
           M+Y+ LDSIT  +IEALGIAS VAEKLH LL+EII SYGNGTPETWRHISKRVLSPDLPF
Sbjct: 1   MEYKALDSITAIEIEALGIASEVAEKLHGLLSEIIRSYGNGTPETWRHISKRVLSPDLPF 60

Query: 280 SFHQMMYYGCYKHYGLDPPAWMPEPENAVFTNVGQLLERRGKEFLGSNYRDPLSSFSSFQ 339
           SFHQMMYYGC+KHYG DPPAWMPEPENAVFTNVGQLLERRGKEFLGSNYRDP SSFSSFQ
Sbjct: 61  SFHQMMYYGCFKHYGPDPPAWMPEPENAVFTNVGQLLERRGKEFLGSNYRDPFSSFSSFQ 120

Query: 340 EFSVSNPEVYWRTVLDEMHITFSKPPKCILQMNGSPESQFPNPGGQWLPGGVFNPAKDCL 399
           EFSVSNPEVYWRTVLDEMHITFSKPP CILQMN SPESQF +PGGQWLPG VFNPAKDCL
Sbjct: 121 EFSVSNPEVYWRTVLDEMHITFSKPPNCILQMNDSPESQFRSPGGQWLPGAVFNPAKDCL 180

Query: 400 SVNENRSLDDIAIIWRDEGCDNLPVKRLTLRELRAEVWLIAHALNSIGFEKGTAIAIDMP 459
           S+NENRSLDD+AIIWRDEGCDNLPVKRLTLRELRAEVWL+AHALNSIGFEKGTAIAIDMP
Sbjct: 181 SINENRSLDDVAIIWRDEGCDNLPVKRLTLRELRAEVWLVAHALNSIGFEKGTAIAIDMP 240

Query: 460 MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRVNISKAKAIFTQDLIIRGDKSIPLYS 519
           MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTR+NISKAKAIFTQDLIIRGDKSIPLYS
Sbjct: 241 MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRLNISKAKAIFTQDLIIRGDKSIPLYS 300

Query: 520 RVVDAQSLLAIVIPTRSTGFSMKLRDGDISWHAFLERVKDLKGVEFSAVEQSAESFTNIL 579
           R++DAQS LAIVIPT STGFSMKLRD DISWHAFLERVKDLKGVEFSAVE+S ESFTNIL
Sbjct: 301 RIIDAQSPLAIVIPTNSTGFSMKLRDSDISWHAFLERVKDLKGVEFSAVEKSGESFTNIL 360

Query: 580 FSSGTTGEPKAIPWTIVTPLKAAADAWCHMDIHKGDVVAWPTNLGWMMGPWLVYASLLNS 639
           FSSGTTGEPKAIPWT+VTPLKAAADAWCHMDI  GDVVAWPTNLGWMMGPWLVYASLLN 
Sbjct: 361 FSSGTTGEPKAIPWTLVTPLKAAADAWCHMDIRHGDVVAWPTNLGWMMGPWLVYASLLNR 420

Query: 640 ASLALYNGSPLGSGFVKFVQDAKITMLGVVPSIVRTWKITNCTSGYDWSSIRCFSSTGEA 699
           AS+ALYNGSPL SGFVKFVQDAK+TMLGV+PSIVR+WK TNCTSGYDWSSIRCF+STGEA
Sbjct: 421 ASMALYNGSPLSSGFVKFVQDAKVTMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGEA 480

Query: 700 STVDECLWLMGRAGYKPVIEICGGTEIGGGFVAGSLLQPQALSAFSTPGMGCSLFILGND 759
           S++DE LWLMGRA YKP+IE CGGTEIGGGFV GSLLQ QAL+AFS P MGCSLFILGND
Sbjct: 481 SSIDEYLWLMGRACYKPIIEYCGGTEIGGGFVTGSLLQAQALAAFSAPAMGCSLFILGND 540

Query: 760 GFPIPQHMPGIGELALGPFLFGASSTLLNADHYDIYFKGMPRWNGMVLRRHGDVFERSPR 819
             PIPQ MPGIGELALGPF+FGASS+LLNADHYD+YFKGMPRWNGM+LRRHGD+FERSPR
Sbjct: 541 DSPIPQSMPGIGELALGPFMFGASSSLLNADHYDVYFKGMPRWNGMILRRHGDLFERSPR 600

Query: 820 GYYRAHGRADDTMNLGGIKVSSVEIERVCNTVDDSILETAAIGVPPHGGGPEQLVIAIVF 879
            YYRAHGRADDTMNLGGIKVSSVEIER+CNTVD+SILETAAIGVPPHGGGPEQLV+ +VF
Sbjct: 601 AYYRAHGRADDTMNLGGIKVSSVEIERICNTVDESILETAAIGVPPHGGGPEQLVVTVVF 660

Query: 880 KNPNETSPDLNKLKLSFNSSLQKNLNPLFRVHRVVPYPSLPRTATNKVMRRILRQQLALE 939
           KNP++TSPDL+KLKLSFNS+LQKNLNPLFRVHRVVPYPSLPRTATNKVMRR+LRQQLA+E
Sbjct: 661 KNPSDTSPDLDKLKLSFNSALQKNLNPLFRVHRVVPYPSLPRTATNKVMRRVLRQQLAIE 720

Query: 940 HRPKL 945
           H+ KL
Sbjct: 721 HKTKL 725

BLAST of Spg022103 vs. ExPASy TrEMBL
Match: A0A6J1I6W7 (probable acyl-activating enzyme 17, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111471706 PE=4 SV=1)

HSP 1 Score: 1357.4 bits (3512), Expect = 0.0e+00
Identity = 656/725 (90.48%), Postives = 690/725 (95.17%), Query Frame = 0

Query: 220 MDYRTLDSITLTDIEALGIASGVAEKLHKLLTEIIHSYGNGTPETWRHISKRVLSPDLPF 279
           MDYR LDSI +TDI+ALGIAS VA+KLH LL EII SYGNG+PETW HISKR+LSPDLPF
Sbjct: 1   MDYRALDSIAVTDIKALGIASEVADKLHGLLAEIIRSYGNGSPETWCHISKRILSPDLPF 60

Query: 280 SFHQMMYYGCYKHYGLDPPAWMPEPENAVFTNVGQLLERRGKEFLGSNYRDPLSSFSSFQ 339
           SFHQMMYYGCYKHYG DPPAWMPE E+AVFTNVGQLL+RRGKEFLGSNY+DP++SFSSFQ
Sbjct: 61  SFHQMMYYGCYKHYGSDPPAWMPELESAVFTNVGQLLDRRGKEFLGSNYKDPITSFSSFQ 120

Query: 340 EFSVSNPEVYWRTVLDEMHITFSKPPKCILQMNGSPESQFPNPGGQWLPGGVFNPAKDCL 399
           EFSVSNPEVYW+ VLD+M ITFSKPPKCILQMN SP+ Q P PGGQWLPG VFNPAKDCL
Sbjct: 121 EFSVSNPEVYWKAVLDDMQITFSKPPKCILQMNDSPDYQSPWPGGQWLPGAVFNPAKDCL 180

Query: 400 SVNENRSLDDIAIIWRDEGCDNLPVKRLTLRELRAEVWLIAHALNSIGFEKGTAIAIDMP 459
           S+NENRSLDD A+IWRDEGCDN+PVKRLTLRELR E WL+AHALNS+GFEKGTAIAIDMP
Sbjct: 181 SLNENRSLDDAALIWRDEGCDNMPVKRLTLRELRTEAWLVAHALNSLGFEKGTAIAIDMP 240

Query: 460 MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRVNISKAKAIFTQDLIIRGDKSIPLYS 519
           MNV +VVIYLAIVLAGHVVVSIADSFAAREISTRVNISKAKAIFTQDLIIRGDKSIPLYS
Sbjct: 241 MNVTSVVIYLAIVLAGHVVVSIADSFAAREISTRVNISKAKAIFTQDLIIRGDKSIPLYS 300

Query: 520 RVVDAQSLLAIVIPTRSTGFSMKLRDGDISWHAFLERVKDLKGVEFSAVEQSAESFTNIL 579
           RVVDAQS LAIVIPTRSTG SMKLRDGDISWHAFLERVKDLKGVEF+AVEQSAESFTNIL
Sbjct: 301 RVVDAQSPLAIVIPTRSTGLSMKLRDGDISWHAFLERVKDLKGVEFAAVEQSAESFTNIL 360

Query: 580 FSSGTTGEPKAIPWTIVTPLKAAADAWCHMDIHKGDVVAWPTNLGWMMGPWLVYASLLNS 639
           FSSGTTGEPKAIPWT+VTPLKAAADAWCHMDIHKGDVVAWPTNLGWMMGPWLVYASLLN 
Sbjct: 361 FSSGTTGEPKAIPWTLVTPLKAAADAWCHMDIHKGDVVAWPTNLGWMMGPWLVYASLLNK 420

Query: 640 ASLALYNGSPLGSGFVKFVQDAKITMLGVVPSIVRTWKITNCTSGYDWSSIRCFSSTGEA 699
           ASLALY+GSPLGSGFVKFVQDAK+TMLGVVPSIVR+WK T CTSGYDWSSIRCFSSTGEA
Sbjct: 421 ASLALYDGSPLGSGFVKFVQDAKVTMLGVVPSIVRSWKSTKCTSGYDWSSIRCFSSTGEA 480

Query: 700 STVDECLWLMGRAGYKPVIEICGGTEIGGGFVAGSLLQPQALSAFSTPGMGCSLFILGND 759
           STVDECLWLMGRAGYKPVIEICGGTEIGGGFVAGSLLQ QALSAFSTPGMGCSLFILGND
Sbjct: 481 STVDECLWLMGRAGYKPVIEICGGTEIGGGFVAGSLLQAQALSAFSTPGMGCSLFILGND 540

Query: 760 GFPIPQHMPGIGELALGPFLFGASSTLLNADHYDIYFKGMPRWNGMVLRRHGDVFERSPR 819
           GFP+PQ+MPGIGELALGPFLFGAS TLLNADHYD+YFKGMPRWNGMVLRRHGDVFERSPR
Sbjct: 541 GFPLPQNMPGIGELALGPFLFGASCTLLNADHYDVYFKGMPRWNGMVLRRHGDVFERSPR 600

Query: 820 GYYRAHGRADDTMNLGGIKVSSVEIERVCNTVDDSILETAAIGVPPHGGGPEQLVIAIVF 879
           GYYRAHGRADDTMNLGGIKVSSVEIER+CN+VD+SILETAAIGVP HGGGPEQL IAIVF
Sbjct: 601 GYYRAHGRADDTMNLGGIKVSSVEIERICNSVDESILETAAIGVPSHGGGPEQLTIAIVF 660

Query: 880 KNPNETSPDLNKLKLSFNSSLQKNLNPLFRVHRVVPYPSLPRTATNKVMRRILRQQLALE 939
           KN + ++PD NKLKL FNSSLQKNLNPLF+VHRVVPYPSLPRTATNKVMRRILRQQLA E
Sbjct: 661 KNQSGSTPDPNKLKLCFNSSLQKNLNPLFKVHRVVPYPSLPRTATNKVMRRILRQQLAAE 720

Query: 940 HRPKL 945
           HR KL
Sbjct: 721 HRAKL 725

BLAST of Spg022103 vs. ExPASy TrEMBL
Match: A0A0A0LUS2 (AMP-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G015740 PE=4 SV=1)

HSP 1 Score: 1313.1 bits (3397), Expect = 0.0e+00
Identity = 630/725 (86.90%), Postives = 688/725 (94.90%), Query Frame = 0

Query: 220 MDYRTLDSITLTDIEALGIASGVAEKLHKLLTEIIHSYGNGTPETWRHISKRVLSPDLPF 279
           MDY+ LDSIT+ DIEALG+ S +A++LH  L EII +YGNGTP TW HISK++L+PDLPF
Sbjct: 1   MDYKPLDSITVIDIEALGVGSQLAKQLHGSLGEIIRTYGNGTPATWLHISKKLLTPDLPF 60

Query: 280 SFHQMMYYGCYKHYGLDPPAWMPEPENAVFTNVGQLLERRGKEFLGSNYRDPLSSFSSFQ 339
           SFHQMMYYGCYK++G DPPAW+PEPENAVFTNVG+LLE+RGKEF+GS+Y DPLSSFSSFQ
Sbjct: 61  SFHQMMYYGCYKNFGPDPPAWIPEPENAVFTNVGKLLEKRGKEFMGSSYSDPLSSFSSFQ 120

Query: 340 EFSVSNPEVYWRTVLDEMHITFSKPPKCILQMNGSPESQFPNPGGQWLPGGVFNPAKDCL 399
           EFSVSNPEVYWRTVLDEM+ITFSKPP CILQ+N SPES+F +PGGQWLPG VFNPAKDCL
Sbjct: 121 EFSVSNPEVYWRTVLDEMNITFSKPPNCILQINDSPESKFSSPGGQWLPGAVFNPAKDCL 180

Query: 400 SVNENRSLDDIAIIWRDEGCDNLPVKRLTLRELRAEVWLIAHALNSIGFEKGTAIAIDMP 459
           S+NENRSLDD+AIIWRDEG D+LP+KRLTLRELRAEVWL+AHALNSIGFEKGTAIAIDMP
Sbjct: 181 SLNENRSLDDVAIIWRDEGYDDLPLKRLTLRELRAEVWLVAHALNSIGFEKGTAIAIDMP 240

Query: 460 MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRVNISKAKAIFTQDLIIRGDKSIPLYS 519
           MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTR++ISKAKAIFTQDLIIRGDKSIPLYS
Sbjct: 241 MNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRLDISKAKAIFTQDLIIRGDKSIPLYS 300

Query: 520 RVVDAQSLLAIVIPTRSTGFSMKLRDGDISWHAFLERVKDLKGVEFSAVEQSAESFTNIL 579
           RVVDAQ+ LAIVIPT STGFSMKLRDGDISWHAFL++VKDLKGVEF AVEQSAESFTNIL
Sbjct: 301 RVVDAQAPLAIVIPTNSTGFSMKLRDGDISWHAFLDKVKDLKGVEFDAVEQSAESFTNIL 360

Query: 580 FSSGTTGEPKAIPWTIVTPLKAAADAWCHMDIHKGDVVAWPTNLGWMMGPWLVYASLLNS 639
           FSSGTTGEPKAIPWT+VTPLKAAADAWCHMDIHKGDVVAWPTNLGWMMGPWLVYASLLN 
Sbjct: 361 FSSGTTGEPKAIPWTLVTPLKAAADAWCHMDIHKGDVVAWPTNLGWMMGPWLVYASLLNK 420

Query: 640 ASLALYNGSPLGSGFVKFVQDAKITMLGVVPSIVRTWKITNCTSGYDWSSIRCFSSTGEA 699
           AS+ALYNGSPL SGFVKF+QDAK TMLGV+PSIVR+WK TNCTSGYDWSSIRCF+STGEA
Sbjct: 421 ASMALYNGSPLSSGFVKFIQDAKTTMLGVIPSIVRSWKSTNCTSGYDWSSIRCFASTGEA 480

Query: 700 STVDECLWLMGRAGYKPVIEICGGTEIGGGFVAGSLLQPQALSAFSTPGMGCSLFILGND 759
           S+VDE LWLMGRA YKP+IE CGGTEIGGGFV GS+LQ QAL+AFSTP MGCSLFILG+D
Sbjct: 481 SSVDEYLWLMGRACYKPIIEYCGGTEIGGGFVTGSMLQAQALAAFSTPAMGCSLFILGDD 540

Query: 760 GFPIPQHMPGIGELALGPFLFGASSTLLNADHYDIYFKGMPRWNGMVLRRHGDVFERSPR 819
           G PIPQ+MPGIGELALGPF+FGASS+LLNADHYDIYFKGMP WNGM++RRHGD+FERSPR
Sbjct: 541 GSPIPQNMPGIGELALGPFMFGASSSLLNADHYDIYFKGMPIWNGMLMRRHGDLFERSPR 600

Query: 820 GYYRAHGRADDTMNLGGIKVSSVEIERVCNTVDDSILETAAIGVPPHGGGPEQLVIAIVF 879
           G+YRAHGRADDTMNLGGIKVSSVEIER+CNTV++SILETAAIGVPP GGGPE+LVIAIVF
Sbjct: 601 GFYRAHGRADDTMNLGGIKVSSVEIERICNTVNNSILETAAIGVPPQGGGPERLVIAIVF 660

Query: 880 KNPNETSPDLNKLKLSFNSSLQKNLNPLFRVHRVVPYPSLPRTATNKVMRRILRQQLALE 939
           KNP+ETS DL+KLK SFNS++QKNLNPLFRVHRVVPYPSLPRTATNKVMRRILRQQL LE
Sbjct: 661 KNPSETSIDLDKLKQSFNSAIQKNLNPLFRVHRVVPYPSLPRTATNKVMRRILRQQLTLE 720

Query: 940 HRPKL 945
           H+ KL
Sbjct: 721 HKTKL 725

BLAST of Spg022103 vs. TAIR 10
Match: AT5G23050.1 (acyl-activating enzyme 17 )

HSP 1 Score: 1047.0 bits (2706), Expect = 9.3e-306
Identity = 502/718 (69.92%), Postives = 604/718 (84.12%), Query Frame = 0

Query: 220 MDYRTLDSITLTDIEALGIASGVAEKLHKLLTEIIHSYGNGT-PETWRHISKRVLSPDLP 279
           M Y++L+SIT +DIEALGI+  V+EKL + L +IIH  G+ T PETW  IS+R+L P+LP
Sbjct: 1   MAYKSLNSITKSDIEALGISGDVSEKLLRDLEDIIH--GSSTPPETWIQISRRILHPNLP 60

Query: 280 FSFHQMMYYGCYKHYGLDPPAWMPEPENAVFTNVGQLLERRGKEFLGSNYRDPLSSFSSF 339
           FSFHQMMYYGCYK +G D PAW+P+P+ A  TNVG+LLE+RGKEFLG NY++P+SSFSSF
Sbjct: 61  FSFHQMMYYGCYKDFGPDLPAWIPDPKVASLTNVGKLLEKRGKEFLGGNYKNPVSSFSSF 120

Query: 340 QEFSVSNPEVYWRTVLDEMHITFSKPPKCILQMNGSPESQFPNPGGQWLPGGVFNPAKDC 399
           QEFSVSNPEVYW+TVLDE++I FS PPKCIL+ + S +    NPGG+WLPG   NPA++C
Sbjct: 121 QEFSVSNPEVYWKTVLDELNILFSVPPKCILEKDTSGD----NPGGKWLPGAYLNPARNC 180

Query: 400 LSVNENRSLDDIAIIWRDEGCDNLPVKRLTLRELRAEVWLIAHALNSIGFEKGTAIAIDM 459
           L+    R LDDI I WRDEG D+LPV  +TL ELR++VWL AHAL+++G E+ +AIA+DM
Sbjct: 181 LTNGFKRRLDDIVIRWRDEGSDDLPVNTMTLLELRSQVWLAAHALSALGLEEESAIAVDM 240

Query: 460 PMNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRVNISKAKAIFTQDLIIRGDKSIPLY 519
           PMNV +V+IYLAIVLAGHVVVSIADSF+ REISTR+ ISKAKAIFTQD+IIRGDKSIPLY
Sbjct: 241 PMNVESVIIYLAIVLAGHVVVSIADSFSPREISTRLKISKAKAIFTQDVIIRGDKSIPLY 300

Query: 520 SRVVDAQSLLAIVIPTRSTGFSMKLRDGDISWHAFLERVKDLKGVEFSAVEQSAESFTNI 579
            RVVDA++ LAIV+P R +   MKLR+ D+SW+ FL   ++L+GVE+ A E+ A ++TNI
Sbjct: 301 RRVVDAEAPLAIVVPARGSSCRMKLREKDLSWNNFLGNARNLRGVEYVAAEKPAGAYTNI 360

Query: 580 LFSSGTTGEPKAIPWTIVTPLKAAADAWCHMDIHKGDVVAWPTNLGWMMGPWLVYASLLN 639
           LFSSGTTGEPKAIPWT ++PLK+AADAWCH+D+ +GDVVAWPTNLGWMMGPWLVYASL+N
Sbjct: 361 LFSSGTTGEPKAIPWTNISPLKSAADAWCHLDVQRGDVVAWPTNLGWMMGPWLVYASLIN 420

Query: 640 SASLALYNGSPLGSGFVKFVQDAKITMLGVVPSIVRTWKITNCTSGYDWSSIRCFSSTGE 699
            A + LYNGSPLG  F KFVQDA++++LGV+PSIVRTW+ +N TSGYDWS IRCF STGE
Sbjct: 421 GACMGLYNGSPLGPTFAKFVQDAEVSVLGVIPSIVRTWQNSNSTSGYDWSRIRCFGSTGE 480

Query: 700 ASTVDECLWLMGRAGYKPVIEICGGTEIGGGFVAGSLLQPQALSAFSTPGMGCSLFILGN 759
           AS +DE LWLMGRA YKPVIE CGGTEIGG F++GSLLQPQ+L+AFST  MGC LFIL  
Sbjct: 481 ASNIDEYLWLMGRAHYKPVIEYCGGTEIGGSFISGSLLQPQSLAAFSTAAMGCKLFILDE 540

Query: 760 DGFPIPQHMPGIGELALGPFLFGASSTLLNADHYDIYFKGMPRWNGMVLRRHGDVFERSP 819
           D  PIP +  G+GELAL P +FGASSTLLN +H+ +YF+GMP + G +LRRHGD+FER+ 
Sbjct: 541 DSNPIPPYAAGVGELALCPHMFGASSTLLNGNHFKVYFQGMPTFQGQILRRHGDLFERTS 600

Query: 820 RGYYRAHGRADDTMNLGGIKVSSVEIERVCNTVDDSILETAAIGVPPHGGGPEQLVIAIV 879
           +GYYRAHGRADDTMNLGGIKV S+EIERVCN+VDDS+LETAAIGVPP  GGPEQLVIA+V
Sbjct: 601 KGYYRAHGRADDTMNLGGIKVGSIEIERVCNSVDDSVLETAAIGVPPPSGGPEQLVIAVV 660

Query: 880 FKNPNETSPDLNKLKLSFNSSLQKNLNPLFRVHRVVPYPSLPRTATNKVMRRILRQQL 937
           FK+P  ++PDLN LK SFNS +QK LNPLF+V  VV  PSLPRTATNKVMRR+LRQQL
Sbjct: 661 FKSPEFSNPDLNLLKKSFNSEIQKKLNPLFKVSSVVTLPSLPRTATNKVMRRVLRQQL 712

BLAST of Spg022103 vs. TAIR 10
Match: AT1G55320.1 (acyl-activating enzyme 18 )

HSP 1 Score: 696.8 bits (1797), Expect = 2.4e-200
Identity = 352/728 (48.35%), Postives = 477/728 (65.52%), Query Frame = 0

Query: 222 YRTLDSITLTDIEALGIASGVAEKLHKLLTEIIHSYGNGTP-ETWRH-ISKRVLSPDLPF 281
           ++++  ++  D    G+    A++  KL++++I       P + W+  + + VL P  P 
Sbjct: 2   WKSIGELSCDDYVKAGLTLEDAKEFDKLVSDVITKAIETDPRDQWKALVDESVLKPWHPH 61

Query: 282 SFHQMMYYGCYKHYGLD----PPAWMPEPENAVFTNVGQLLERRGKEFLGSNYRDPLSSF 341
             HQ++YY  Y ++       P  W P    +  TN+G+L+E  G   LG +Y++PL SF
Sbjct: 62  PLHQLLYYSVYSNWDSSVHGPPLYWFPSLSQSKSTNLGKLMEYHGPRLLGPSYKNPLESF 121

Query: 342 SSFQEFSVSNPEVYWRTVLDEMHITFSKPPKCILQMNGSPESQFPNPGGQWLPGGVFNPA 401
             F+ FSV +PEVYW  V+DE+ + F  PP+CIL  +         P G WLP  V N A
Sbjct: 122 ELFRRFSVEHPEVYWSFVIDELSLVFHTPPRCILNKS--------KPEGTWLPDAVLNIA 181

Query: 402 KDCL--SVNENRSLDDIAIIWRDEGCDNLPVKRLTLRELRAEVWLIAHALNSIGFEKGTA 461
           + CL  S +  +  D +A++WR+EG D+ PV R+T++ELR +V L+A+A++   FEKG  
Sbjct: 182 ECCLMPSSHPKKEDDSVAVVWRNEGFDDSPVNRMTIKELREQVMLVANAISG-SFEKGDT 241

Query: 462 IAIDMPMNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRVNISKAKAIFTQDLIIRGDK 521
           IAIDMPM V+AV+IYLAI+LAG +VVSIADSFAA+EI+TR+ ISKAK IFTQD I+RG +
Sbjct: 242 IAIDMPMTVDAVIIYLAIILAGCIVVSIADSFAAKEIATRLKISKAKGIFTQDYILRGGR 301

Query: 522 SIPLYSRVVDAQSLLAIVIPTRSTGFSMKLRDGDISWHAFLERVKDLKGVE--FSAVEQS 581
             PLYSRVV+A     IV+P   T   ++LR+ D+SW  FL   K     E  +  +   
Sbjct: 302 RFPLYSRVVEAAPSKVIVLPASGTELHVQLREQDVSWMDFLSNAKPHSSGENYYRPIYLP 361

Query: 582 AESFTNILFSSGTTGEPKAIPWTIVTPLKAAADAWCHMDIHKGDVVAWPTNLGWMMGPWL 641
            ES  NILFSSGTTGEPKAIPWT ++P+++A D W H+D+  G    WPTNLGW+MGP L
Sbjct: 362 VESVINILFSSGTTGEPKAIPWTQLSPIRSACDGWAHLDVQVGHTYCWPTNLGWVMGPTL 421

Query: 642 VYASLLNSASLALYNGSPLGSGFVKFVQDAKITMLGVVPSIVRTWKITNCTSGYDWSSIR 701
           +++  L  A+LALY+GSPLG GF KFVQDA +T+LG VPS+V+TWK TNC  G +W+ I+
Sbjct: 422 MFSCFLTGATLALYSGSPLGRGFGKFVQDAGVTVLGTVPSLVKTWKRTNCMEGLNWTKIK 481

Query: 702 CFSSTGEASTVDECLWLMGRAGYKPVIEICGGTEIGGGFVAGSLLQPQALSAFSTPGMGC 761
            F++TGEAS VD+ LWL  +A YKPVIE CGGTE+   ++ GS LQPQA  AFSTP M  
Sbjct: 482 FFATTGEASNVDDVLWLSSKADYKPVIECCGGTELASSYIIGSPLQPQAFGAFSTPSMTT 541

Query: 762 SLFILGNDGFPIPQHMPGIGELALGPFLFGASSTLLNADHYDIYFKGMPRWNGMVLRRHG 821
            + I   +G P P   P  GE+ L P   GA+  LLNA+H ++YFKGMP +    LRRHG
Sbjct: 542 RIIIFDENGVPYPDDQPCTGEVGLFPQHLGATDRLLNANHDEVYFKGMPMYKETRLRRHG 601

Query: 822 DVFERSPRGYYRAHGRADDTMNLGGIKVSSVEIERVCNTVDDSILETAAIGVPPHGGGPE 881
           D+ +R+  GYY   GRADDTMNLGGIK SS+EIERVC+  D+ I ETAA+ + P  GGPE
Sbjct: 602 DIVKRTVGGYYNVQGRADDTMNLGGIKTSSIEIERVCDQADECISETAAVTLTPPNGGPE 661

Query: 882 QLVIAIVFKNPNETSPDLNKLKLSFNSSLQKNLNPLFRVHRVVPYPSLPRTATNKVMRRI 940
            LVI  V K   +      +LK+ F+ ++QK+LNPLF+V  V   P  PRTA++K++RR+
Sbjct: 662 LLVIFAVLKEGFKQQSG-EELKMKFSRTIQKDLNPLFKVSFVKIVPEFPRTASSKLLRRV 719

BLAST of Spg022103 vs. TAIR 10
Match: AT1G55320.2 (acyl-activating enzyme 18 )

HSP 1 Score: 548.5 bits (1412), Expect = 1.0e-155
Identity = 275/504 (54.56%), Postives = 353/504 (70.04%), Query Frame = 0

Query: 438 LIAHALNSIGFEKGTAIAIDMPMNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRVNIS 497
           L+A+A++   FEKG  IAIDMPM V+AV+IYLAI+LAG +VVSIADSFAA+EI+TR+ IS
Sbjct: 2   LVANAISG-SFEKGDTIAIDMPMTVDAVIIYLAIILAGCIVVSIADSFAAKEIATRLKIS 61

Query: 498 KAKAIFTQDLIIRGDKSIPLYSRVVDAQSLLAIVIPTRSTGFSMKLRDGDISWHAFLERV 557
           KAK IFTQD I+RG +  PLYSRVV+A     IV+P   T   ++LR+ D+SW  FL   
Sbjct: 62  KAKGIFTQDYILRGGRRFPLYSRVVEAAPSKVIVLPASGTELHVQLREQDVSWMDFLSNA 121

Query: 558 KDLKGVE--FSAVEQSAESFTNILFSSGTTGEPKAIPWTIVTPLKAAADAWCHMDIHKGD 617
           K     E  +  +    ES  NILFSSGTTGEPKAIPWT ++P+++A D W H+D+  G 
Sbjct: 122 KPHSSGENYYRPIYLPVESVINILFSSGTTGEPKAIPWTQLSPIRSACDGWAHLDVQVGH 181

Query: 618 VVAWPTNLGWMMGPWLVYASLLNSASLALYNGSPLGSGFVKFVQDAKITMLGVVPSIVRT 677
              WPTNLGW+MGP L+++  L  A+LALY+GSPLG GF KFVQDA +T+LG VPS+V+T
Sbjct: 182 TYCWPTNLGWVMGPTLMFSCFLTGATLALYSGSPLGRGFGKFVQDAGVTVLGTVPSLVKT 241

Query: 678 WKITNCTSGYDWSSIRCFSSTGEASTVDECLWLMGRAGYKPVIEICGGTEIGGGFVAGSL 737
           WK TNC  G +W+ I+ F++TGEAS VD+ LWL  +A YKPVIE CGGTE+   ++ GS 
Sbjct: 242 WKRTNCMEGLNWTKIKFFATTGEASNVDDVLWLSSKADYKPVIECCGGTELASSYIIGSP 301

Query: 738 LQPQALSAFSTPGMGCSLFILGNDGFPIPQHMPGIGELALGPFLFGASSTLLNADHYDIY 797
           LQPQA  AFSTP M   + I   +G P P   P  GE+ L P   GA+  LLNA+H ++Y
Sbjct: 302 LQPQAFGAFSTPSMTTRIIIFDENGVPYPDDQPCTGEVGLFPQHLGATDRLLNANHDEVY 361

Query: 798 FKGMPRWNGMVLRRHGDVFERSPRGYYRAHGRADDTMNLGGIKVSSVEIERVCNTVDDSI 857
           FKGMP +    LRRHGD+ +R+  GYY   GRADDTMNLGGIK SS+EIERVC+  D+ I
Sbjct: 362 FKGMPMYKETRLRRHGDIVKRTVGGYYNVQGRADDTMNLGGIKTSSIEIERVCDQADECI 421

Query: 858 LETAAIGVPPHGGGPEQLVIAIVFKNPNETSPDLNKLKLSFNSSLQKNLNPLFRVHRVVP 917
            ETAA+ + P  GGPE LVI  V K   +      +LK+ F+ ++QK+LNPLF+V  V  
Sbjct: 422 SETAAVTLTPPNGGPELLVIFAVLKEGFKQQSG-EELKMKFSRTIQKDLNPLFKVSFVKI 481

Query: 918 YPSLPRTATNKVMRRILRQQLALE 940
            P  PRTA++K++RR+LR Q+  E
Sbjct: 482 VPEFPRTASSKLLRRVLRDQIKQE 503

BLAST of Spg022103 vs. TAIR 10
Match: AT5G36880.1 (acetyl-CoA synthetase )

HSP 1 Score: 129.4 bits (324), Expect = 1.5e-29
Identity = 164/644 (25.47%), Postives = 262/644 (40.68%), Query Frame = 0

Query: 321 KEFLGSNYRDPLSSFSSFQEFSVSNPEVYWRTVLDEMHITFSKPPKCI---LQMNGSPES 380
           KEF G         +    + S+ +P  +W  +  E +       +     L +   P S
Sbjct: 46  KEFSGQALVSSPQQYMEMHKRSMDDPAAFWSDIASEFYWKQKWGDQVFSENLDVRKGPIS 105

Query: 381 QFPNPGGQWLPGGVFNPAKDCLSVNENRSL-DDIAIIWRDEGCDNLPVKRLTLRELRAEV 440
                  +W  GG+ N   +CL  N    L D  AI W  EG +      LT  EL   V
Sbjct: 106 I------EWFKGGITNICYNCLDKNVEAGLGDKTAIHW--EGNELGVDASLTYSELLQRV 165

Query: 441 WLIAHALNSIGFEKGTAIAIDMPMNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRVNI 500
             +A+ L   G +KG A+ I +PM +   +  LA    G V   +   F+A  ++ R+  
Sbjct: 166 CQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVD 225

Query: 501 SKAKAIFTQDLIIRGDKSIPLYSRVVDA---QS---------LLAIVIPTRSTGFSMKLR 560
            K   I T + + RG K+I L   +VDA   QS          L       +T  + K +
Sbjct: 226 CKPNVILTCNAVKRGPKTINL-KAIVDAALDQSSKDGVSVGICLTYDNSLATTRENTKWQ 285

Query: 561 DG-DISWHAFLERVKDLKGVEFSAVEQSAESFTNILFSSGTTGEPKAIPWTIVTPLKAAA 620
           +G D+ W   + +      VE+      AE    +L++SG+TG+PK +  T    +   A
Sbjct: 286 NGRDVWWQDVISQYPTSCEVEW----VDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTA 345

Query: 621 DAWCHMDIHKGDVVAWPT-NLGWMMG-PWLVYASLLNSASLALYNGSPL---GSGFVKFV 680
             + +   +K   V W T + GW+ G  ++ Y  +LN A++ ++ G+P           V
Sbjct: 346 TTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFEGAPNYPDPGRCWDIV 405

Query: 681 QDAKITMLGVVPSIVRTWKITN--CTSGYDWSSIRCFSSTGEASTVDECLWLMGRAGYK- 740
              K+++    P++VR+    +    + +   S+R   S GE        W     G   
Sbjct: 406 DKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGDSR 465

Query: 741 -PVIEICGGTEIGGGFVAGSL--LQPQALSAFSTPGMGCSLFILGNDGFPIPQHMPGIGE 800
            P+ +    TE  GGF+   L    PQ   + + P  G    I+   G  I     G   
Sbjct: 466 CPISDTWWQTET-GGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGY-- 525

Query: 801 LALGPFLFGASSTLL-NADHYD-IYFKGMPRWNGMVLRRHGDVFERSPRGYYRAHGRADD 860
           L +     GA  TL  + + Y+  YFK    +        GD   R   GYY   GR DD
Sbjct: 526 LCVKGSWPGAFRTLFGDHERYETTYFKPFAGY-----YFSGDGCSRDKDGYYWLTGRVDD 585

Query: 861 TMNLGGIKVSSVEIERVCNTVDDSILETAAIGVPPHGGGPEQLVIAIVFKNPNETSPDLN 920
            +N+ G ++ + E+E     +     E A +G+     G  Q + A  F    E  P   
Sbjct: 586 VINVSGHRIGTAEVESAL-VLHPQCAEAAVVGIEHEVKG--QGIYA--FVTLLEGVPYSE 645

Query: 921 KLKLSFNSSLQKNLNPLFRVHRVVPYPSLPRTATNKVMRRILRQ 935
           +L+ S    ++  +       R+   P LP+T + K+MRRILR+
Sbjct: 646 ELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 663

BLAST of Spg022103 vs. TAIR 10
Match: AT5G36880.2 (acetyl-CoA synthetase )

HSP 1 Score: 129.4 bits (324), Expect = 1.5e-29
Identity = 164/644 (25.47%), Postives = 262/644 (40.68%), Query Frame = 0

Query: 321 KEFLGSNYRDPLSSFSSFQEFSVSNPEVYWRTVLDEMHITFSKPPKCI---LQMNGSPES 380
           KEF G         +    + S+ +P  +W  +  E +       +     L +   P S
Sbjct: 96  KEFSGQALVSSPQQYMEMHKRSMDDPAAFWSDIASEFYWKQKWGDQVFSENLDVRKGPIS 155

Query: 381 QFPNPGGQWLPGGVFNPAKDCLSVNENRSL-DDIAIIWRDEGCDNLPVKRLTLRELRAEV 440
                  +W  GG+ N   +CL  N    L D  AI W  EG +      LT  EL   V
Sbjct: 156 I------EWFKGGITNICYNCLDKNVEAGLGDKTAIHW--EGNELGVDASLTYSELLQRV 215

Query: 441 WLIAHALNSIGFEKGTAIAIDMPMNVNAVVIYLAIVLAGHVVVSIADSFAAREISTRVNI 500
             +A+ L   G +KG A+ I +PM +   +  LA    G V   +   F+A  ++ R+  
Sbjct: 216 CQLANYLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVD 275

Query: 501 SKAKAIFTQDLIIRGDKSIPLYSRVVDA---QS---------LLAIVIPTRSTGFSMKLR 560
            K   I T + + RG K+I L   +VDA   QS          L       +T  + K +
Sbjct: 276 CKPNVILTCNAVKRGPKTINL-KAIVDAALDQSSKDGVSVGICLTYDNSLATTRENTKWQ 335

Query: 561 DG-DISWHAFLERVKDLKGVEFSAVEQSAESFTNILFSSGTTGEPKAIPWTIVTPLKAAA 620
           +G D+ W   + +      VE+      AE    +L++SG+TG+PK +  T    +   A
Sbjct: 336 NGRDVWWQDVISQYPTSCEVEW----VDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTA 395

Query: 621 DAWCHMDIHKGDVVAWPT-NLGWMMG-PWLVYASLLNSASLALYNGSPL---GSGFVKFV 680
             + +   +K   V W T + GW+ G  ++ Y  +LN A++ ++ G+P           V
Sbjct: 396 TTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFEGAPNYPDPGRCWDIV 455

Query: 681 QDAKITMLGVVPSIVRTWKITN--CTSGYDWSSIRCFSSTGEASTVDECLWLMGRAGYK- 740
              K+++    P++VR+    +    + +   S+R   S GE        W     G   
Sbjct: 456 DKYKVSIFYTAPTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGDSR 515

Query: 741 -PVIEICGGTEIGGGFVAGSL--LQPQALSAFSTPGMGCSLFILGNDGFPIPQHMPGIGE 800
            P+ +    TE  GGF+   L    PQ   + + P  G    I+   G  I     G   
Sbjct: 516 CPISDTWWQTET-GGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGY-- 575

Query: 801 LALGPFLFGASSTLL-NADHYD-IYFKGMPRWNGMVLRRHGDVFERSPRGYYRAHGRADD 860
           L +     GA  TL  + + Y+  YFK    +        GD   R   GYY   GR DD
Sbjct: 576 LCVKGSWPGAFRTLFGDHERYETTYFKPFAGY-----YFSGDGCSRDKDGYYWLTGRVDD 635

Query: 861 TMNLGGIKVSSVEIERVCNTVDDSILETAAIGVPPHGGGPEQLVIAIVFKNPNETSPDLN 920
            +N+ G ++ + E+E     +     E A +G+     G  Q + A  F    E  P   
Sbjct: 636 VINVSGHRIGTAEVESAL-VLHPQCAEAAVVGIEHEVKG--QGIYA--FVTLLEGVPYSE 695

Query: 921 KLKLSFNSSLQKNLNPLFRVHRVVPYPSLPRTATNKVMRRILRQ 935
           +L+ S    ++  +       R+   P LP+T + K+MRRILR+
Sbjct: 696 ELRKSLVLMVRNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713