Spg022100 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg022100
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	(1->3)-beta-glucan endohydrolase
Location	scaffold2: 9075494 .. 9076480 (+)
RNA-Seq Expression	Spg022100
Synteny	Spg022100

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATTGTCTTCTTCACGGCTGCCATGTCAGCGGTCGTCGGAGCATACGATGTTCTTCTCGGTGCCTATTACGGTCTCGAGGCCAACAACTTGCCACCGCCGTGGAAGGTGGTGCAACTTTGCCTAAAACACAACATCCGCCGCGTCCAATTGGAAGAACCGAACCTCGACGTCTTCCAAGCGTTTCGTGGCACAGGAATCGACCTCTCTTTTAGCATTCCCAACGACATGTTACCAAACATGGCCACCAATAACTCCGCCGTGGAGGAGTGGTTTAACACCTACGTCAAACCTTTCACCGACAGTTTCACCATCAATTACATCGTCGTCGGGGATAGGGCCCTCCCCGGCCTCGACGACAGCATATTGCCCGTCATGAAATCCCTTCAAAATCTCCTCAACGCTAACTACCTCGGACAAGTGAAAGTCACCACATTTGTTGGCTACAAATCTGTATTGTCAGTGAAAGATCTTCCATCCAGCAGCACGTTTAGCCCAAACATTGCCGAGACCATGAGAGGAATCCTTCAGTTCCTGTCTGGAGAAGGGTCGCCACTGATGATCAGCATAGATCCATATCAGAAATATTTTTATAGCGGCACTATAAGTTTGGATTACGCCACGTTTGGCAAGGCGACCCCGGTGCACGATGGCACGCTAACTTATTACAACTTGTTTGATGAAATGGTGGATGCATTTTATGTTGCGATCGATAAGGCAAAAGTCGGAAATGTGGCCATTGCAGTGGGGCAAATTGGGTGGCCGACTTGTGGGAACGCTAACATCGCAACGACGTCGCTTGCGGCCAAGTTCAACCAGAATTTTAAGAGGCATATTGTGTCTGGCAAAGGGACACCAATGAAGCAGAATGTTTATATGGAGGGATTCGTTCATAGTATATTCAACGAGAATCAAAAGCCTGAAGGGGAGCCACGATGTTATGGCATATTCAATGTTGACTCCACACCTATTTATCCTTTGTTTTAA

mRNA sequence

ATGATTGTCTTCTTCACGGCTGCCATGTCAGCGGTCGTCGGAGCATACGATGTTCTTCTCGGTGCCTATTACGGTCTCGAGGCCAACAACTTGCCACCGCCGTGGAAGGTGGTGCAACTTTGCCTAAAACACAACATCCGCCGCGTCCAATTGGAAGAACCGAACCTCGACGTCTTCCAAGCGTTTCGTGGCACAGGAATCGACCTCTCTTTTAGCATTCCCAACGACATGTTACCAAACATGGCCACCAATAACTCCGCCGTGGAGGAGTGGTTTAACACCTACGTCAAACCTTTCACCGACAGTTTCACCATCAATTACATCGTCGTCGGGGATAGGGCCCTCCCCGGCCTCGACGACAGCATATTGCCCGTCATGAAATCCCTTCAAAATCTCCTCAACGCTAACTACCTCGGACAAGTGAAAGTCACCACATTTGTTGGCTACAAATCTGTATTGTCAGTGAAAGATCTTCCATCCAGCAGCACGTTTAGCCCAAACATTGCCGAGACCATGAGAGGAATCCTTCAGTTCCTGTCTGGAGAAGGGTCGCCACTGATGATCAGCATAGATCCATATCAGAAATATTTTTATAGCGGCACTATAAGTTTGGATTACGCCACGTTTGGCAAGGCGACCCCGGTGCACGATGGCACGCTAACTTATTACAACTTGTTTGATGAAATGGTGGATGCATTTTATGTTGCGATCGATAAGGCAAAAGTCGGAAATGTGGCCATTGCAGTGGGGCAAATTGGGTGGCCGACTTGTGGGAACGCTAACATCGCAACGACGTCGCTTGCGGCCAAGTTCAACCAGAATTTTAAGAGGCATATTGTGTCTGGCAAAGGGACACCAATGAAGCAGAATGTTTATATGGAGGGATTCGTTCATAGTATATTCAACGAGAATCAAAAGCCTGAAGGGGAGCCACGATGTTATGGCATATTCAATGTTGACTCCACACCTATTTATCCTTTGTTTTAA

Coding sequence (CDS)

ATGATTGTCTTCTTCACGGCTGCCATGTCAGCGGTCGTCGGAGCATACGATGTTCTTCTCGGTGCCTATTACGGTCTCGAGGCCAACAACTTGCCACCGCCGTGGAAGGTGGTGCAACTTTGCCTAAAACACAACATCCGCCGCGTCCAATTGGAAGAACCGAACCTCGACGTCTTCCAAGCGTTTCGTGGCACAGGAATCGACCTCTCTTTTAGCATTCCCAACGACATGTTACCAAACATGGCCACCAATAACTCCGCCGTGGAGGAGTGGTTTAACACCTACGTCAAACCTTTCACCGACAGTTTCACCATCAATTACATCGTCGTCGGGGATAGGGCCCTCCCCGGCCTCGACGACAGCATATTGCCCGTCATGAAATCCCTTCAAAATCTCCTCAACGCTAACTACCTCGGACAAGTGAAAGTCACCACATTTGTTGGCTACAAATCTGTATTGTCAGTGAAAGATCTTCCATCCAGCAGCACGTTTAGCCCAAACATTGCCGAGACCATGAGAGGAATCCTTCAGTTCCTGTCTGGAGAAGGGTCGCCACTGATGATCAGCATAGATCCATATCAGAAATATTTTTATAGCGGCACTATAAGTTTGGATTACGCCACGTTTGGCAAGGCGACCCCGGTGCACGATGGCACGCTAACTTATTACAACTTGTTTGATGAAATGGTGGATGCATTTTATGTTGCGATCGATAAGGCAAAAGTCGGAAATGTGGCCATTGCAGTGGGGCAAATTGGGTGGCCGACTTGTGGGAACGCTAACATCGCAACGACGTCGCTTGCGGCCAAGTTCAACCAGAATTTTAAGAGGCATATTGTGTCTGGCAAAGGGACACCAATGAAGCAGAATGTTTATATGGAGGGATTCGTTCATAGTATATTCAACGAGAATCAAAAGCCTGAAGGGGAGCCACGATGTTATGGCATATTCAATGTTGACTCCACACCTATTTATCCTTTGTTTTAA

Protein sequence

MIVFFTAAMSAVVGAYDVLLGAYYGLEANNLPPPWKVVQLCLKHNIRRVQLEEPNLDVFQAFRGTGIDLSFSIPNDMLPNMATNNSAVEEWFNTYVKPFTDSFTINYIVVGDRALPGLDDSILPVMKSLQNLLNANYLGQVKVTTFVGYKSVLSVKDLPSSSTFSPNIAETMRGILQFLSGEGSPLMISIDPYQKYFYSGTISLDYATFGKATPVHDGTLTYYNLFDEMVDAFYVAIDKAKVGNVAIAVGQIGWPTCGNANIATTSLAAKFNQNFKRHIVSGKGTPMKQNVYMEGFVHSIFNENQKPEGEPRCYGIFNVDSTPIYPLF

Homology

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BLAST of Spg022100 vs. NCBI nr
Match: KAG6590489.1 (putative glucan endo-1,3-beta-glucosidase BG5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 455.3 bits (1170), Expect = 4.5e-124
Identity = 224/330 (67.88%), Postives = 265/330 (80.30%), Query Frame = 0


Query: 1   MIVFFTAAMSAVVGAYDVLLGAYYGLEANNLPPPWKVVQLCLKHNIRRVQLEEPNLDVFQ 60
           MI  F A MS VVGAYDVLLGAYYGL  +NLP P  VVQLC K+NI RV+LE PNLDV +
Sbjct: 1   MIGLFMADMSLVVGAYDVLLGAYYGLLGDNLPSPSSVVQLCRKYNISRVRLEVPNLDVIE 60

Query: 61  AFRGTGIDLSFSIPNDMLPNMATNNSAVEEWFNTYVKPFTDSFTINYIVVGDRALPGLDD 120
            FRGT IDLSF +PNDMLPNMATN + VEEWFNTYV+P+T+  TINYIVVGD+A+PGLDD
Sbjct: 61  GFRGTEIDLSFGVPNDMLPNMATNKTLVEEWFNTYVEPYTNDCTINYIVVGDKAIPGLDD 120

Query: 121 SILPVMKSLQNLLNANYLGQVKVTTFVGYKSVLSVKDLPSSSTFSPNIAETMRGILQFLS 180
           +ILPVMKSLQ+LLNA+Y G+VK+TT VG+ + L+V + PSS  F+P I E MRGIL+FL 
Sbjct: 121 NILPVMKSLQDLLNAHYFGEVKLTTLVGF-NALAVTNPPSSGAFNPAIVENMRGILEFLR 180

Query: 181 GEGSPLMISIDPYQKYFYSGTISLDYATFGKA-TPVHDGTLTYYNLFDEMVDAFYVAIDK 240
           GEGSPLM+S+  Y +Y Y G ISL YAT  +  T V DG L+Y NLFDE+VDAFY A+DK
Sbjct: 181 GEGSPLMVSVYTYDQYVYGGNISLGYATLSEQNTLVRDGMLSYNNLFDEIVDAFYAAMDK 240

Query: 241 AKVGNVAIAVGQIGWPTCGNANIATTSLAAKFNQNFKRHIVSGKGTPMKQNVYMEGFVHS 300
             VG+VAI  G+ GWP+CGN N  T  LAA++N+NFK HI SGKGTP K+N+Y+EGF+ S
Sbjct: 241 ENVGDVAIVFGETGWPSCGNYNATTPLLAAEYNRNFKNHISSGKGTPRKENIYIEGFIRS 300

Query: 301 IFNENQKPEGEPRCYGIFNVDSTPIY-PLF 329
           IFNENQKPEGE RCYG+FNVDSTPIY P+F
Sbjct: 301 IFNENQKPEGESRCYGMFNVDSTPIYSPVF 329

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BLAST of Spg022100 vs. NCBI nr
Match: XP_022136187.1 (probable glucan endo-1,3-beta-glucosidase BG5 [Momordica charantia])

HSP 1 Score: 454.5 bits (1168), Expect = 7.6e-124
Identity = 220/330 (66.67%), Postives = 264/330 (80.00%), Query Frame = 0


Query: 1   MIVFFTAAMSAVVGAYDVLLGAYYGLEANNLPPPWKVVQLCLKHNIRRVQLEEPNLDVFQ 60
           +IVFF A+MSA V A DVLLG YYGL  +N+PPPWKVVQLC+K+NIRR++L+EPNL+VF 
Sbjct: 7   IIVFFMASMSA-VRANDVLLGVYYGLFGDNVPPPWKVVQLCVKYNIRRIRLDEPNLEVFD 66

Query: 61  AFRGTGIDLSFSIPNDMLPNMATNNSAVEEWFNTYVKPFTDSFTINYIVVGDRALPGLDD 120
           AF G GID+SF +PNDML NM  N SAV EWFNTYV+P+   F INYI+VG++A+PGLDD
Sbjct: 67  AFCGAGIDISFGVPNDMLINMKANESAVVEWFNTYVEPYIGDFVINYIIVGNKAIPGLDD 126

Query: 121 SILPVMKSLQNLLNANYLGQVKVTTFVGYKSVLSVKDLPSSSTFSPNIAETMRGILQFLS 180
            ILPVMKSLQNLLNA YLGQVK+TT VGY + L+VKD PSS  F PN ++ +R IL+FL 
Sbjct: 127 CILPVMKSLQNLLNARYLGQVKLTTLVGYNAALAVKDPPSSGAFDPNASDNIREILRFLL 186

Query: 181 GEGSPLMISIDPYQKYFYSG-TISLDYATFGKATP-VHDGTLTYYNLFDEMVDAFYVAID 240
            EGSPLM+S+ PY KY YS   ISL+YATFG  TP VHDG L+Y NLFD MVD FY A+D
Sbjct: 187 REGSPLMVSVCPYTKYAYSNRAISLNYATFGAKTPVVHDGGLSYDNLFDAMVDPFYAAMD 246

Query: 241 KAKVGNVAIAVGQIGWPTCGNANIATTSLAAKFNQNFKRHIVSGKGTPMKQNVYMEGFVH 300
           K  VG+V +AVG+ GWPTCGN NIAT   A+ +N+NFK HI SG+GTP + NV + GF+ 
Sbjct: 247 KLGVGDVDVAVGETGWPTCGNGNIATPQYASAYNKNFKSHISSGRGTPKRPNVLIRGFIK 306

Query: 301 SIFNENQKPEGEPRCYGIFNVDSTPIYPLF 329
           S+FNEN+ PEGE +CYGIFNVDSTP+YPLF
Sbjct: 307 SMFNENKMPEGESQCYGIFNVDSTPLYPLF 335

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BLAST of Spg022100 vs. NCBI nr
Match: XP_022968751.1 (probable glucan endo-1,3-beta-glucosidase BG4 [Cucurbita maxima])

HSP 1 Score: 442.6 bits (1137), Expect = 3.0e-120
Identity = 217/330 (65.76%), Postives = 259/330 (78.48%), Query Frame = 0


Query: 1   MIVFFTAAMSAVVGAYDVLLGAYYGLEANNLPPPWKVVQLCLKHNIRRVQLEEPNLDVFQ 60
           MI  F A M  +VGAYDVLLGAYYGL  +NL  P  VVQLC K+NI RV+LE  N+DV +
Sbjct: 7   MIGLFMADMFLIVGAYDVLLGAYYGLLGDNLSSPASVVQLCRKYNISRVRLEVSNIDVIE 66

Query: 61  AFRGTGIDLSFSIPNDMLPNMATNNSAVEEWFNTYVKPFTDSFTINYIVVGDRALPGLDD 120
           AFRGT IDLS  +PNDMLPNMATN + VEEWFNTYV+P+ + + INYIVVGD+A+PGLDD
Sbjct: 67  AFRGTEIDLSLGVPNDMLPNMATNKTLVEEWFNTYVEPYVNDYIINYIVVGDKAIPGLDD 126

Query: 121 SILPVMKSLQNLLNANYLGQVKVTTFVGYKSVLSVKDLPSSSTFSPNIAETMRGILQFLS 180
           +ILPVMKSLQ+LLNA+Y G+ K+TT VG+ + L+V + PSS  F+P I E MRGIL+FL 
Sbjct: 127 NILPVMKSLQDLLNAHYFGEAKLTTLVGF-NALAVTNPPSSGAFNPAIVENMRGILEFLR 186

Query: 181 GEGSPLMISIDPYQKYFYSGTISLDYATFGKATP-VHDGTLTYYNLFDEMVDAFYVAIDK 240
           GEGSPLM+S+  Y +Y Y G  SL YAT  +    V DG L+Y NLFDEMVDAFY AIDK
Sbjct: 187 GEGSPLMVSVYTYDQYVYGGNRSLGYATLSEQNSLVRDGMLSYNNLFDEMVDAFYAAIDK 246

Query: 241 AKVGNVAIAVGQIGWPTCGNANIATTSLAAKFNQNFKRHIVSGKGTPMKQNVYMEGFVHS 300
             VG+VAI +G+ GWPTCGN N  T  LAA++N+NFK HI SGKGTP K+N+Y+EGF+ S
Sbjct: 247 ENVGDVAIVIGETGWPTCGNYNATTPLLAAEYNRNFKSHISSGKGTPRKENIYIEGFIRS 306

Query: 301 IFNENQKPEGEPRCYGIFNVDSTPIY-PLF 329
           IFNENQKPEGE RCYG+FNVDSTPIY P+F
Sbjct: 307 IFNENQKPEGESRCYGMFNVDSTPIYSPVF 335

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BLAST of Spg022100 vs. NCBI nr
Match: XP_038880685.1 (probable glucan endo-1,3-beta-glucosidase BG5 [Benincasa hispida])

HSP 1 Score: 439.9 bits (1130), Expect = 1.9e-119
Identity = 213/330 (64.55%), Postives = 261/330 (79.09%), Query Frame = 0


Query: 1   MIVFFTAAMSAVVGAYDVLLGAYYGLEANNLPPPWKVVQLCLKHNIRRVQLEEPNLDVFQ 60
           +IV F   +S++VGAYDVLLGAYY L  NNLPPPW+VVQLC K+NIRRV+L EPNL V +
Sbjct: 7   IIVIFMVFVSSIVGAYDVLLGAYYRLAGNNLPPPWEVVQLCKKYNIRRVRLYEPNLTVLE 66

Query: 61  AFRGTGIDLSFSIPNDMLPNMATNNSAVEEWFNTYVKPFTDSFTINYIVVGDRALPGLDD 120
           +FRG GID+SF +PN+++ NMATN++AVEEWF  YV PF   FTINYIVVGD+ +PGLD 
Sbjct: 67  SFRGMGIDVSFGVPNNLIENMATNHTAVEEWFINYVAPFIGDFTINYIVVGDKVIPGLDG 126

Query: 121 SILPVMKSLQNLLNANYLGQVKVTTFVGYKSVLSVKDLPSSSTFSPNIAETMRGILQFLS 180
            IL VM+SLQ LLNA  LGQVK+TT V   + LS +  PSS  F P IA  MRGIL FL 
Sbjct: 127 RILQVMQSLQGLLNARNLGQVKLTTLVSI-TALSAQSPPSSGAFDPTIAANMRGILHFLW 186

Query: 181 GEGSPLMISIDPYQKYFYSGTISLDYATFGKAT-PVHDGTLTYYNLFDEMVDAFYVAIDK 240
            +GSPLM+S+ P++ YFYSG +SL YATF +    + DG L+Y NLFDEM+DAFY AIDK
Sbjct: 187 EQGSPLMVSLYPHKTYFYSGNMSLGYATFAEENFSIRDGDLSYNNLFDEMMDAFYAAIDK 246

Query: 241 AKVGNVAIAVGQIGWPTCGNANIATTSLAAKFNQNFKRHIVSGKGTPMKQNVYMEGFVHS 300
             VG+VAIAVG+ GWPTCG+ NI TTS+AA +N+NFK HI SGKGTPMK N+Y++GF+ S
Sbjct: 247 EDVGDVAIAVGETGWPTCGHLNITTTSIAATYNRNFKNHITSGKGTPMKSNIYIQGFIKS 306

Query: 301 IFNENQKPEGEPRCYGIFNVDSTPIY-PLF 329
           IFNEN++PEGE +CYG+F+VDSTPIY P+F
Sbjct: 307 IFNENEEPEGESKCYGMFHVDSTPIYSPVF 335

[Close]

BLAST of Spg022100 vs. NCBI nr
Match: TYK10032.1 (lichenase-2-like [Cucumis melo var. makuwa])

HSP 1 Score: 434.1 bits (1115), Expect = 1.1e-117
Identity = 214/333 (64.26%), Postives = 265/333 (79.58%), Query Frame = 0


Query: 2   IVFFTAAMSAVVGAYDVLLGAYYGLEANNLPPPWKVVQLCLKHNIRRVQLEEPNLDVFQA 61
           ++F  A  S VVGAY++LLGAYYGLE+NNLP PWKV+QLC K+NIRR++++EPNLDV ++
Sbjct: 11  LLFMVAMSSIVVGAYNILLGAYYGLESNNLPTPWKVMQLCEKYNIRRIRIDEPNLDVIES 70

Query: 62  FRGTGIDLSFSIPNDMLPNMATNNSAVEEWFNTYVKPFTDSFTINYIVVGDRALPGLDDS 121
           FRGT I++SF + N ++ NMATN++AVEEWF  Y+ PF D  TINYIVVGD+A+PGLD+S
Sbjct: 71  FRGTNIEVSFGVSNGLITNMATNHTAVEEWFANYIAPFIDDLTINYIVVGDKAVPGLDNS 130

Query: 122 ILPVMKSLQNLLNANYLGQVKVTTFVGYKSVLSVKDLPSSSTFSPNIAETMRGILQFLSG 181
           ILPVMKSLQ+LLN  +LGQVK+TT VG  S L VK  PSS  F PN+ E MRGILQFL G
Sbjct: 131 ILPVMKSLQDLLNRRFLGQVKITTLVGL-SALGVKAPPSSGAFDPNVFENMRGILQFLWG 190

Query: 182 EGSPLMISIDPYQKYFYSGTISLDYATF----GKATPVH-DGTLTYYNLFDEMVDAFYVA 241
           +GSPLM+S++PY+ Y Y+  ISL YATF     +  P+  DG L+Y NLFDE+VDAFY A
Sbjct: 191 QGSPLMLSLNPYEAYAYN-NISLGYATFTPQLEQNPPIRIDGNLSYNNLFDEVVDAFYAA 250

Query: 242 IDKAKVGNVAIAVGQIGWPTCGNANIATTSLAAKFNQNFKRHIVSGKGTPMKQNVYMEGF 301
           IDKA VG+VAIA+G+ GWPT G  + A+  LAA +N NFK HI SGKGTPMK N+Y+EGF
Sbjct: 251 IDKASVGHVAIAIGETGWPTNGGNDGASPKLAATYNINFKNHISSGKGTPMKPNIYIEGF 310

Query: 302 VHSIFNENQKPEGEPRCYGIFNVDSTPIY-PLF 329
           + S+FNEN+KPEGE R YG+FNVDSTPIY P+F
Sbjct: 311 IKSLFNENEKPEGESRFYGMFNVDSTPIYSPVF 341

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BLAST of Spg022100 vs. ExPASy Swiss-Prot
Match: O49353 (Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN=BG5 PE=2 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 1.0e-54
Identity = 119/314 (37.90%), Postives = 185/314 (58.92%), Query Frame = 0


Query: 20  LGAYYGLEANNLPPPWKVVQLCLKHNIRRVQLEEPNLDVFQAFRG-TGIDLSFSIPNDML 79
           +G  YGL  +NLP P KV+ L    +I ++++ +PN +V  A RG   I ++  + +  L
Sbjct: 43  IGLNYGLLGDNLPSPSKVITLYKSIDITKIRIFDPNTEVLNALRGHRDIAVTVGVRDQDL 102

Query: 80  PNMATNNSAVEEWFNTYVKPFTDSFTINYIVVGDRALPG-LDDSILPVMKSLQNLLNANY 139
             ++ +  AV+ WF T ++P+     I +I VG+  +PG +   +LPVM+SL NL+ +  
Sbjct: 103 AALSASEEAVKGWFATNIEPYLSDINIAFITVGNEVIPGPIGPQVLPVMQSLTNLVKSRN 162

Query: 140 LGQVKVTTFVGYKSVLSVKDLPSSSTFSPNIAETMRGILQFLSGEGSPLMISIDPYQKYF 199
           L  + ++T V   + L     PS+  F+    E +  +L+ LS   SP+++ I PY  Y 
Sbjct: 163 L-PISISTVVAMWN-LEQSYPPSAGMFTSQAREQLVPVLKLLSQTNSPILVKIYPYFSYA 222

Query: 200 YS-GTISLDYATFG-KATPVHDGTLTYYNLFDEMVDAFYVAIDKAKVGNVAIAVGQIGWP 259
               +I LDYATF  +A  V DG+L Y N+FD + DAF  A++K  V ++ + V + GWP
Sbjct: 223 SDPSSIRLDYATFNTEAIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKDLPMVVSETGWP 282

Query: 260 TCGNANIATTSLAAKFNQNFKRHIVSGKGTPMKQNVYMEGFVHSIFNENQKPEGEPRCYG 319
           + GN NI T  +A  +N+NF +HI SGKGTP + N  ++GF+ + FNENQKP G  + +G
Sbjct: 283 SAGNGNITTPDIAGTYNRNFVKHIASGKGTPKRPNKGIDGFLFATFNENQKPVGTEQNFG 342

Query: 320 IFNV-DSTPIYPLF 329
           ++N  D  PIY LF
Sbjct: 343 LYNPNDMKPIYNLF 354

[Close]

BLAST of Spg022100 vs. ExPASy Swiss-Prot
Match: O49352 (Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN=BG4 PE=2 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 3.0e-54
Identity = 118/314 (37.58%), Postives = 182/314 (57.96%), Query Frame = 0


Query: 20  LGAYYGLEANNLPPPWKVVQLCLKHNIRRVQLEEPNLDVFQAFRG-TGIDLSFSIPNDML 79
           +G  YGL  +NLP P  V+ L     I R+++ +PN +V  A RG   I+++  + +  L
Sbjct: 34  IGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFDPNTEVLNALRGHRDIEVTVGVKDQDL 93

Query: 80  PNMATNNSAVEEWFNTYVKPFTDSFTINYIVVGDRALPG-LDDSILPVMKSLQNLLNANY 139
             +A +  AV+ WF   ++ +     I +I VG+  +PG +   +LPVM+SL NL+ +  
Sbjct: 94  AALAASEEAVKGWFAANIESYLADVNITFITVGNEVIPGPIGPQVLPVMQSLTNLVKSRN 153

Query: 140 LGQVKVTTFVGYKSVLSVKDLPSSSTFSPNIAETMRGILQFLSGEGSPLMISIDPYQKYF 199
           L  + ++T V   S L     PS+  F+    E +  +L+ LS   +P++++I PY  Y 
Sbjct: 154 L-PISISTVVA-MSNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTSTPILVNIYPYFAYA 213

Query: 200 YS-GTISLDYATFG-KATPVHDGTLTYYNLFDEMVDAFYVAIDKAKVGNVAIAVGQIGWP 259
                I LDYA+F  K+  V DG+L Y N+FD + DAF  A++K  V N+ + V + GWP
Sbjct: 214 SDPANIRLDYASFNTKSIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKNLPMVVSETGWP 273

Query: 260 TCGNANIATTSLAAKFNQNFKRHIVSGKGTPMKQNVYMEGFVHSIFNENQKPEGEPRCYG 319
           + GN N  T ++A+ +N+NF +HI SGKGTP + N  M GF+ + FNENQKP G  + +G
Sbjct: 274 SAGNGNFTTPAIASTYNRNFVKHIASGKGTPKRPNKSMNGFLFATFNENQKPAGTEQNFG 333

Query: 320 IFN-VDSTPIYPLF 329
           ++N  D  PIY LF
Sbjct: 334 LYNPSDMKPIYKLF 345

[Close]

BLAST of Spg022100 vs. ExPASy Swiss-Prot
Match: O22317 (Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2)

HSP 1 Score: 210.3 bits (534), Expect = 3.3e-53
Identity = 117/313 (37.38%), Postives = 175/313 (55.91%), Query Frame = 0


Query: 20  LGAYYGLEANNLPPPWKVVQLCLKHNIRRVQLEEPNLDVFQAFRGTGIDLSFSIPNDMLP 79
           +G  YG+  NNLPPP +VV L   +NI R++L +PN    QA R + I +   +P   + 
Sbjct: 29  IGVCYGMLGNNLPPPSEVVSLYKSNNIARMRLYDPNQAALQALRNSNIQVLLDVPRSDVQ 88

Query: 80  NMATNNSAVEEWFNTYVKPFTDSFTINYIVVGDRALPGLD--DSILPVMKSLQNLL-NAN 139
           ++A+N SA  +W    V  +  S +  YI VG+  +PG D    ILP M+++ N L +A 
Sbjct: 89  SLASNPSAAGDWIRRNVVAYWPSVSFRYIAVGNELIPGSDLAQYILPAMRNIYNALSSAG 148

Query: 140 YLGQVKVTTFVGYKSVLSVKDLPSSSTFSPNIAETMRGILQFLSGEGSPLMISIDPYQKY 199
              Q+KV+T V    VL     PS+  FS      +  I+QFL+  G+PL++++ PY  Y
Sbjct: 149 LQNQIKVSTAVD-TGVLGTSYPPSAGAFSSAAQAYLSPIVQFLASNGAPLLVNVYPYFSY 208

Query: 200 FYS-GTISLDYATF-GKATPVHDGTLTYYNLFDEMVDAFYVAIDKAKVGNVAIAVGQIGW 259
             + G ISL YA F      V DG  +Y NLFD +VDA + A+++    NVA+ V + GW
Sbjct: 209 TGNPGQISLPYALFTASGVVVQDGRFSYQNLFDAIVDAVFAALERVGGANVAVVVSESGW 268

Query: 260 PTCGNANIATTSLAAKFNQNFKRHIVSGKGTPMKQNVYMEGFVHSIFNENQKPEGEPRCY 319
           P+ G    A+TS A  +NQN  RH+  G GTP +    +E ++  +FNENQK  G  + +
Sbjct: 269 PSAGGGAEASTSNARTYNQNLIRHV--GGGTPRRPGKEIEAYIFEMFNENQKAGGIEQNF 328

Query: 320 GIFNVDSTPIYPL 328
           G+F  +  P+Y +
Sbjct: 329 GLFYPNKQPVYQI 338

[Close]

BLAST of Spg022100 vs. ExPASy Swiss-Prot
Match: P12257 (Lichenase-2 (Fragment) OS=Hordeum vulgare OX=4513 PE=1 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 3.9e-46
Identity = 108/310 (34.84%), Postives = 170/310 (54.84%), Query Frame = 0


Query: 20  LGAYYGLEANNLPPPWKVVQLCLKHNIRRVQLEEPNLDVFQAFRGTGIDLSFSIPNDMLP 79
           +G  YG+ ANNLP    VV +   + I+ ++L  PN    QA  GTGI++    PND+L 
Sbjct: 7   IGVCYGMSANNLPAASTVVSMFKFNGIKSMRLYAPNQAALQAVGGTGINVVVGAPNDVLS 66

Query: 80  NMATNNSAVEEWFNTYVKPFTDSFTINYIVVGDRALPGLDDSILPVMKSLQNLLNANYLG 139
           N+A + +A   W  + ++ +    +  Y+ VG+    G   +++P MK++   L A  LG
Sbjct: 67  NLAASPAAAASWVKSNIQAY-PKVSFRYVCVGNEVAGGATRNLVPAMKNVHGALVAAGLG 126

Query: 140 QVKVTTFVGYKSVLSVKDLPSSSTFSPNIAETMRGILQFLSGEGSPLMISIDPYQKYFYS 199
            +KVTT V  +++L V   PS+ +F+   A  M  ++QFL+   +PLM +I PY  + Y+
Sbjct: 127 HIKVTTSVS-QAILGVFSPPSAGSFTGEAAAFMGPVVQFLARTNAPLMANIYPYLAWAYN 186

Query: 200 -GTISLDYATF-GKATPVHDGTLTYYNLFDEMVDAFYVAIDKAKVGNVAIAVGQIGWPTC 259
              + + YA F    T V DG   Y NLFD  VDAFY A+ K    +V + V + GWP+ 
Sbjct: 187 PSAMDMGYALFNASGTVVRDGAYGYQNLFDTTVDAFYTAMGKHGGSSVKLVVSESGWPS- 246

Query: 260 GNANIATTSLAAKFNQNFKRHIVSGKGTPMKQNVYMEGFVHSIFNENQKPEGEPRCYGIF 319
           G    AT + A  +NQ+   H+  G+GTP      +E ++ ++FNENQK  G  + +G+F
Sbjct: 247 GGGTAATPANARFYNQHLINHV--GRGTPRHPGA-IETYIFAMFNENQKDSGVEQNWGLF 306

Query: 320 NVDSTPIYPL 328
             +   +YP+
Sbjct: 307 YPNMQHVYPI 310

[Close]

BLAST of Spg022100 vs. ExPASy Swiss-Prot
Match: P07979 (Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3)

HSP 1 Score: 183.7 bits (465), Expect = 3.3e-45
Identity = 115/326 (35.28%), Postives = 179/326 (54.91%), Query Frame = 0


Query: 12  VVGAYDVLLGAYYGLEANNLPPPWKVVQLCLKHNIRRVQLEEPNLDVFQAFRGTGIDLSF 71
           +VGA  V  G  YG+  NNLPP  +VVQL    NIRR++L +PN    QA RG+ I++  
Sbjct: 27  IVGAQSV--GVCYGMLGNNLPPASQVVQLYKSKNIRRMRLYDPNQAALQALRGSNIEVML 86

Query: 72  SIPNDMLPNMATNNSAVEEWFNTYVKPFTDSFTINYIVVGDRALP-----GLDDSILPVM 131
            +PN  L N+A N S    W    V+ F  +    YI VG+   P      L   +LP M
Sbjct: 87  GVPNSDLQNIAANPSNANNWVQRNVRNFWPAVKFRYIAVGNEVSPVTGTSSLTRYLLPAM 146

Query: 132 KSLQNLL-NANYLGQVKVTTFVGYKSVLSVKDLPSSSTFSPNIAETMRGILQFLSGEGSP 191
           ++++N + +A     +KV++ V   +++     PS  +F  ++   +  I+ F+    SP
Sbjct: 147 RNIRNAISSAGLQNNIKVSSSVD-MTLIGNSFPPSQGSFRNDVRSFIDPIIGFVRRINSP 206

Query: 192 LMISIDPYQKYFYSGT---ISLDYATF-GKATPVHDGTLTYYNLFDEMVDAFYVAIDKAK 251
           L+++I PY  + Y+G    ISL YA F      V DG+L Y NLFD M DA Y A+ +A 
Sbjct: 207 LLVNIYPY--FSYAGNPRDISLPYALFTAPNVVVQDGSLGYRNLFDAMSDAVYAALSRAG 266

Query: 252 VGNVAIAVGQIGWPTCGNANIATTSLAAKFNQNFKRHIVSGKGTPMKQNVYMEGFVHSIF 311
            G++ I V + GWP+ G A  ATT+ AA + +N  +H+   +G+P + N  +E ++ ++F
Sbjct: 267 GGSIEIVVSESGWPSAG-AFAATTNNAATYYKNLIQHV--KRGSPRRPNKVIETYLFAMF 326

Query: 312 NENQKPEGEPRCYGIFNVDSTPIYPL 328
           +EN K     + +G+F+ +  P YPL
Sbjct: 327 DENNKNPELEKHFGLFSPNKQPKYPL 344

[Close]

BLAST of Spg022100 vs. ExPASy TrEMBL
Match: A0A6J1C4V7 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111007943 PE=3 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 3.7e-124
Identity = 220/330 (66.67%), Postives = 264/330 (80.00%), Query Frame = 0


Query: 1   MIVFFTAAMSAVVGAYDVLLGAYYGLEANNLPPPWKVVQLCLKHNIRRVQLEEPNLDVFQ 60
           +IVFF A+MSA V A DVLLG YYGL  +N+PPPWKVVQLC+K+NIRR++L+EPNL+VF 
Sbjct: 7   IIVFFMASMSA-VRANDVLLGVYYGLFGDNVPPPWKVVQLCVKYNIRRIRLDEPNLEVFD 66

Query: 61  AFRGTGIDLSFSIPNDMLPNMATNNSAVEEWFNTYVKPFTDSFTINYIVVGDRALPGLDD 120
           AF G GID+SF +PNDML NM  N SAV EWFNTYV+P+   F INYI+VG++A+PGLDD
Sbjct: 67  AFCGAGIDISFGVPNDMLINMKANESAVVEWFNTYVEPYIGDFVINYIIVGNKAIPGLDD 126

Query: 121 SILPVMKSLQNLLNANYLGQVKVTTFVGYKSVLSVKDLPSSSTFSPNIAETMRGILQFLS 180
            ILPVMKSLQNLLNA YLGQVK+TT VGY + L+VKD PSS  F PN ++ +R IL+FL 
Sbjct: 127 CILPVMKSLQNLLNARYLGQVKLTTLVGYNAALAVKDPPSSGAFDPNASDNIREILRFLL 186

Query: 181 GEGSPLMISIDPYQKYFYSG-TISLDYATFGKATP-VHDGTLTYYNLFDEMVDAFYVAID 240
            EGSPLM+S+ PY KY YS   ISL+YATFG  TP VHDG L+Y NLFD MVD FY A+D
Sbjct: 187 REGSPLMVSVCPYTKYAYSNRAISLNYATFGAKTPVVHDGGLSYDNLFDAMVDPFYAAMD 246

Query: 241 KAKVGNVAIAVGQIGWPTCGNANIATTSLAAKFNQNFKRHIVSGKGTPMKQNVYMEGFVH 300
           K  VG+V +AVG+ GWPTCGN NIAT   A+ +N+NFK HI SG+GTP + NV + GF+ 
Sbjct: 247 KLGVGDVDVAVGETGWPTCGNGNIATPQYASAYNKNFKSHISSGRGTPKRPNVLIRGFIK 306

Query: 301 SIFNENQKPEGEPRCYGIFNVDSTPIYPLF 329
           S+FNEN+ PEGE +CYGIFNVDSTP+YPLF
Sbjct: 307 SMFNENKMPEGESQCYGIFNVDSTPLYPLF 335

[Close]

BLAST of Spg022100 vs. ExPASy TrEMBL
Match: A0A6J1HVR6 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111467895 PE=3 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 1.5e-120
Identity = 217/330 (65.76%), Postives = 259/330 (78.48%), Query Frame = 0


Query: 1   MIVFFTAAMSAVVGAYDVLLGAYYGLEANNLPPPWKVVQLCLKHNIRRVQLEEPNLDVFQ 60
           MI  F A M  +VGAYDVLLGAYYGL  +NL  P  VVQLC K+NI RV+LE  N+DV +
Sbjct: 7   MIGLFMADMFLIVGAYDVLLGAYYGLLGDNLSSPASVVQLCRKYNISRVRLEVSNIDVIE 66

Query: 61  AFRGTGIDLSFSIPNDMLPNMATNNSAVEEWFNTYVKPFTDSFTINYIVVGDRALPGLDD 120
           AFRGT IDLS  +PNDMLPNMATN + VEEWFNTYV+P+ + + INYIVVGD+A+PGLDD
Sbjct: 67  AFRGTEIDLSLGVPNDMLPNMATNKTLVEEWFNTYVEPYVNDYIINYIVVGDKAIPGLDD 126

Query: 121 SILPVMKSLQNLLNANYLGQVKVTTFVGYKSVLSVKDLPSSSTFSPNIAETMRGILQFLS 180
           +ILPVMKSLQ+LLNA+Y G+ K+TT VG+ + L+V + PSS  F+P I E MRGIL+FL 
Sbjct: 127 NILPVMKSLQDLLNAHYFGEAKLTTLVGF-NALAVTNPPSSGAFNPAIVENMRGILEFLR 186

Query: 181 GEGSPLMISIDPYQKYFYSGTISLDYATFGKATP-VHDGTLTYYNLFDEMVDAFYVAIDK 240
           GEGSPLM+S+  Y +Y Y G  SL YAT  +    V DG L+Y NLFDEMVDAFY AIDK
Sbjct: 187 GEGSPLMVSVYTYDQYVYGGNRSLGYATLSEQNSLVRDGMLSYNNLFDEMVDAFYAAIDK 246

Query: 241 AKVGNVAIAVGQIGWPTCGNANIATTSLAAKFNQNFKRHIVSGKGTPMKQNVYMEGFVHS 300
             VG+VAI +G+ GWPTCGN N  T  LAA++N+NFK HI SGKGTP K+N+Y+EGF+ S
Sbjct: 247 ENVGDVAIVIGETGWPTCGNYNATTPLLAAEYNRNFKSHISSGKGTPRKENIYIEGFIRS 306

Query: 301 IFNENQKPEGEPRCYGIFNVDSTPIY-PLF 329
           IFNENQKPEGE RCYG+FNVDSTPIY P+F
Sbjct: 307 IFNENQKPEGESRCYGMFNVDSTPIYSPVF 335

[Close]

BLAST of Spg022100 vs. ExPASy TrEMBL
Match: A0A5D3CFE1 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001650 PE=3 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 5.2e-118
Identity = 214/333 (64.26%), Postives = 265/333 (79.58%), Query Frame = 0


Query: 2   IVFFTAAMSAVVGAYDVLLGAYYGLEANNLPPPWKVVQLCLKHNIRRVQLEEPNLDVFQA 61
           ++F  A  S VVGAY++LLGAYYGLE+NNLP PWKV+QLC K+NIRR++++EPNLDV ++
Sbjct: 11  LLFMVAMSSIVVGAYNILLGAYYGLESNNLPTPWKVMQLCEKYNIRRIRIDEPNLDVIES 70

Query: 62  FRGTGIDLSFSIPNDMLPNMATNNSAVEEWFNTYVKPFTDSFTINYIVVGDRALPGLDDS 121
           FRGT I++SF + N ++ NMATN++AVEEWF  Y+ PF D  TINYIVVGD+A+PGLD+S
Sbjct: 71  FRGTNIEVSFGVSNGLITNMATNHTAVEEWFANYIAPFIDDLTINYIVVGDKAVPGLDNS 130

Query: 122 ILPVMKSLQNLLNANYLGQVKVTTFVGYKSVLSVKDLPSSSTFSPNIAETMRGILQFLSG 181
           ILPVMKSLQ+LLN  +LGQVK+TT VG  S L VK  PSS  F PN+ E MRGILQFL G
Sbjct: 131 ILPVMKSLQDLLNRRFLGQVKITTLVGL-SALGVKAPPSSGAFDPNVFENMRGILQFLWG 190

Query: 182 EGSPLMISIDPYQKYFYSGTISLDYATF----GKATPVH-DGTLTYYNLFDEMVDAFYVA 241
           +GSPLM+S++PY+ Y Y+  ISL YATF     +  P+  DG L+Y NLFDE+VDAFY A
Sbjct: 191 QGSPLMLSLNPYEAYAYN-NISLGYATFTPQLEQNPPIRIDGNLSYNNLFDEVVDAFYAA 250

Query: 242 IDKAKVGNVAIAVGQIGWPTCGNANIATTSLAAKFNQNFKRHIVSGKGTPMKQNVYMEGF 301
           IDKA VG+VAIA+G+ GWPT G  + A+  LAA +N NFK HI SGKGTPMK N+Y+EGF
Sbjct: 251 IDKASVGHVAIAIGETGWPTNGGNDGASPKLAATYNINFKNHISSGKGTPMKPNIYIEGF 310

Query: 302 VHSIFNENQKPEGEPRCYGIFNVDSTPIY-PLF 329
           + S+FNEN+KPEGE R YG+FNVDSTPIY P+F
Sbjct: 311 IKSLFNENEKPEGESRFYGMFNVDSTPIYSPVF 341

[Close]

BLAST of Spg022100 vs. ExPASy TrEMBL
Match: A0A5A7UQE4 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G001680 PE=3 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 1.2e-117
Identity = 214/333 (64.26%), Postives = 264/333 (79.28%), Query Frame = 0


Query: 2   IVFFTAAMSAVVGAYDVLLGAYYGLEANNLPPPWKVVQLCLKHNIRRVQLEEPNLDVFQA 61
           ++F     S V+GAY+VLLGAYYGLE+NNLP PWKV+QLC K+NIRR++++EPNLDV ++
Sbjct: 11  LLFMVDMSSIVLGAYNVLLGAYYGLESNNLPTPWKVMQLCEKYNIRRIRIDEPNLDVIES 70

Query: 62  FRGTGIDLSFSIPNDMLPNMATNNSAVEEWFNTYVKPFTDSFTINYIVVGDRALPGLDDS 121
           FRGT I++SF + N ++ NMATN++AVEEWF  Y+ PF D  TINYIVVGD+A+PGLDDS
Sbjct: 71  FRGTNIEVSFGVSNGLITNMATNHTAVEEWFANYIAPFIDDLTINYIVVGDKAVPGLDDS 130

Query: 122 ILPVMKSLQNLLNANYLGQVKVTTFVGYKSVLSVKDLPSSSTFSPNIAETMRGILQFLSG 181
           ILPVMKSLQ+LLN  +LGQVK+TT VG  S L VK  PSS  F PN+ E MRGILQFL G
Sbjct: 131 ILPVMKSLQDLLNRRFLGQVKITTLVGL-SALGVKAPPSSGAFDPNVFENMRGILQFLWG 190

Query: 182 EGSPLMISIDPYQKYFYSGTISLDYATF----GKATPVH-DGTLTYYNLFDEMVDAFYVA 241
           +GSPLM+S++PY+ Y Y+  ISL YATF     +  P+  DG L+Y NLFDE+VDAFY A
Sbjct: 191 QGSPLMLSLNPYEAYAYN-NISLGYATFTPQLEQNPPIRIDGNLSYNNLFDEVVDAFYAA 250

Query: 242 IDKAKVGNVAIAVGQIGWPTCGNANIATTSLAAKFNQNFKRHIVSGKGTPMKQNVYMEGF 301
           IDKA VG+VAIA+G+ GWPT G  + A+  LAA +N NFK HI SGKGTPMK N+Y+EGF
Sbjct: 251 IDKASVGHVAIAIGETGWPTNGGNDGASPKLAATYNINFKNHISSGKGTPMKPNIYIEGF 310

Query: 302 VHSIFNENQKPEGEPRCYGIFNVDSTPIY-PLF 329
           + S+FNEN+KPEGE R YG+FNVDSTPIY P+F
Sbjct: 311 IKSLFNENEKPEGESRFYGMFNVDSTPIYSPVF 341

[Close]

BLAST of Spg022100 vs. ExPASy TrEMBL
Match: A0A5A7UIV1 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G001690 PE=3 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 7.0e-115
Identity = 214/337 (63.50%), Postives = 255/337 (75.67%), Query Frame = 0


Query: 1   MIVFFTAAMSAVVGAYDVLLGAYYGLEANNLPPPWKVVQLCLKHNIRRVQLEEPNLDVFQ 60
           ++ F  A  S VV AYDVLLGAYYG E NNLPPP KVVQLC K+NIRR++  EPN D+  
Sbjct: 11  LLYFMVAMSSIVVRAYDVLLGAYYGTEGNNLPPPEKVVQLCQKYNIRRIRFSEPNFDIID 70

Query: 61  AFRGTGIDLSFSIPNDMLPNMATNNSAVEEWFNTYVKPFTDSFTINYIVVGDRALPGLDD 120
           AFRG  I+LSFS+  +++ NMATN++AVEEWF  YV PF   FTINYI+VGD+A+PGLDD
Sbjct: 71  AFRGKDIELSFSVTGELITNMATNHTAVEEWFVNYVAPFIGDFTINYIIVGDKAIPGLDD 130

Query: 121 SILPVMKSLQNLLNANYLGQVKVTTFVGYKSVLSVKDLPSSSTFSPNIAETMRGILQFLS 180
           +ILPVMKSLQ LLN  YLGQVK+TT VG  + L V+  PSS TF PN+ E MRGILQFL 
Sbjct: 131 NILPVMKSLQVLLNGRYLGQVKITTLVGL-AALGVQTPPSSGTFDPNVLENMRGILQFLH 190

Query: 181 GEGSPLMISIDPYQKYFYSG---TISLDYATFG----KATPVHD-GTLTYYNLFDEMVDA 240
           G+GSPLM+S+ PYQ+Y Y+G    ISL YA F     K  P+   G L+Y NLFDEMVD 
Sbjct: 191 GQGSPLMLSLYPYQEYAYTGNSNNISLGYANFNSQLEKNPPIRTYGDLSYNNLFDEMVDT 250

Query: 241 FYVAIDKAKVGNVAIAVGQIGWPTCGNANIATTSLAAKFNQNFKRHIVSGKGTPMKQNVY 300
           FY AIDKA VG+V I +G+ GWPT GN    + SLAA +N+NFK HI SGKGTPMK N+Y
Sbjct: 251 FYAAIDKANVGDVPIVIGETGWPTNGNYG-GSPSLAATYNRNFKNHISSGKGTPMKPNIY 310

Query: 301 MEGFVHSIFNENQKPEGEPRCYGIFNVDSTPIY-PLF 329
           +EGF+ S+FNEN+KPEGE R YG+FNVDSTPIY P+F
Sbjct: 311 IEGFIRSLFNENEKPEGESRFYGMFNVDSTPIYSPVF 345

[Close]

BLAST of Spg022100 vs. TAIR 10
Match: AT5G20340.1 (beta-1,3-glucanase 5 )

HSP 1 Score: 215.3 bits (547), Expect = 7.3e-56
Identity = 119/314 (37.90%), Postives = 185/314 (58.92%), Query Frame = 0


Query: 20  LGAYYGLEANNLPPPWKVVQLCLKHNIRRVQLEEPNLDVFQAFRG-TGIDLSFSIPNDML 79
           +G  YGL  +NLP P KV+ L    +I ++++ +PN +V  A RG   I ++  + +  L
Sbjct: 43  IGLNYGLLGDNLPSPSKVITLYKSIDITKIRIFDPNTEVLNALRGHRDIAVTVGVRDQDL 102

Query: 80  PNMATNNSAVEEWFNTYVKPFTDSFTINYIVVGDRALPG-LDDSILPVMKSLQNLLNANY 139
             ++ +  AV+ WF T ++P+     I +I VG+  +PG +   +LPVM+SL NL+ +  
Sbjct: 103 AALSASEEAVKGWFATNIEPYLSDINIAFITVGNEVIPGPIGPQVLPVMQSLTNLVKSRN 162

Query: 140 LGQVKVTTFVGYKSVLSVKDLPSSSTFSPNIAETMRGILQFLSGEGSPLMISIDPYQKYF 199
           L  + ++T V   + L     PS+  F+    E +  +L+ LS   SP+++ I PY  Y 
Sbjct: 163 L-PISISTVVAMWN-LEQSYPPSAGMFTSQAREQLVPVLKLLSQTNSPILVKIYPYFSYA 222

Query: 200 YS-GTISLDYATFG-KATPVHDGTLTYYNLFDEMVDAFYVAIDKAKVGNVAIAVGQIGWP 259
               +I LDYATF  +A  V DG+L Y N+FD + DAF  A++K  V ++ + V + GWP
Sbjct: 223 SDPSSIRLDYATFNTEAIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKDLPMVVSETGWP 282

Query: 260 TCGNANIATTSLAAKFNQNFKRHIVSGKGTPMKQNVYMEGFVHSIFNENQKPEGEPRCYG 319
           + GN NI T  +A  +N+NF +HI SGKGTP + N  ++GF+ + FNENQKP G  + +G
Sbjct: 283 SAGNGNITTPDIAGTYNRNFVKHIASGKGTPKRPNKGIDGFLFATFNENQKPVGTEQNFG 342

Query: 320 IFNV-DSTPIYPLF 329
           ++N  D  PIY LF
Sbjct: 343 LYNPNDMKPIYNLF 354

[Close]

BLAST of Spg022100 vs. TAIR 10
Match: AT5G20330.1 (beta-1,3-glucanase 4 )

HSP 1 Score: 213.8 bits (543), Expect = 2.1e-55
Identity = 118/314 (37.58%), Postives = 182/314 (57.96%), Query Frame = 0


Query: 20  LGAYYGLEANNLPPPWKVVQLCLKHNIRRVQLEEPNLDVFQAFRG-TGIDLSFSIPNDML 79
           +G  YGL  +NLP P  V+ L     I R+++ +PN +V  A RG   I+++  + +  L
Sbjct: 34  IGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFDPNTEVLNALRGHRDIEVTVGVKDQDL 93

Query: 80  PNMATNNSAVEEWFNTYVKPFTDSFTINYIVVGDRALPG-LDDSILPVMKSLQNLLNANY 139
             +A +  AV+ WF   ++ +     I +I VG+  +PG +   +LPVM+SL NL+ +  
Sbjct: 94  AALAASEEAVKGWFAANIESYLADVNITFITVGNEVIPGPIGPQVLPVMQSLTNLVKSRN 153

Query: 140 LGQVKVTTFVGYKSVLSVKDLPSSSTFSPNIAETMRGILQFLSGEGSPLMISIDPYQKYF 199
           L  + ++T V   S L     PS+  F+    E +  +L+ LS   +P++++I PY  Y 
Sbjct: 154 L-PISISTVVA-MSNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTSTPILVNIYPYFAYA 213

Query: 200 YS-GTISLDYATFG-KATPVHDGTLTYYNLFDEMVDAFYVAIDKAKVGNVAIAVGQIGWP 259
                I LDYA+F  K+  V DG+L Y N+FD + DAF  A++K  V N+ + V + GWP
Sbjct: 214 SDPANIRLDYASFNTKSIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKNLPMVVSETGWP 273

Query: 260 TCGNANIATTSLAAKFNQNFKRHIVSGKGTPMKQNVYMEGFVHSIFNENQKPEGEPRCYG 319
           + GN N  T ++A+ +N+NF +HI SGKGTP + N  M GF+ + FNENQKP G  + +G
Sbjct: 274 SAGNGNFTTPAIASTYNRNFVKHIASGKGTPKRPNKSMNGFLFATFNENQKPAGTEQNFG 333

Query: 320 IFN-VDSTPIYPLF 329
           ++N  D  PIY LF
Sbjct: 334 LYNPSDMKPIYKLF 345

[Close]

BLAST of Spg022100 vs. TAIR 10
Match: AT5G20390.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 208.4 bits (529), Expect = 8.9e-54
Identity = 114/314 (36.31%), Postives = 177/314 (56.37%), Query Frame = 0


Query: 20  LGAYYGLEANNLPPPWKVVQLCLKHNIRRVQLEEPNLDVFQAFRGT-GIDLSFSIPNDML 79
           +G  YGL  +NLPPP +V+ L    ++  +++ +   DV  AFRG   I +   + N  L
Sbjct: 33  IGLNYGLLGDNLPPPSEVINLYKSLSVTNIRIFDTTTDVLNAFRGNRNIGVMVDVKNQDL 92

Query: 80  PNMATNNSAVEEWFNTYVKPFTDSFTINYIVVGDRALPG-LDDSILPVMKSLQNLLNANY 139
             ++ +  AV  WF T ++P+     I +I VG+  +PG +   +LPVMKSL N++ +  
Sbjct: 93  EALSVSEEAVNTWFVTNIEPYLADVNITFIAVGNEVIPGEIGSYVLPVMKSLTNIVKSRS 152

Query: 140 LGQVKVTTFVGYKSVLSVKDLPSSSTFSPNIAETMRGILQFLSGEGSPLMISIDPYQKYF 199
           L  + ++T V   + L     PS+  F P   E +  +L+FLS   +P++++I PY  Y 
Sbjct: 153 L-PILISTTVAMTN-LGQSYPPSAGDFMPQAREQLTPVLKFLSQTNTPILVNIYPYFAYA 212

Query: 200 YSG-TISLDYATFG-KATPVHDGTLTYYNLFDEMVDAFYVAIDKAKVGNVAIAVGQIGWP 259
                I LDYA F      V DG L Y N+FD + DAF  A++K  V ++ + V + GWP
Sbjct: 213 ADPINIQLDYAIFNTNKVVVQDGPLGYTNMFDVIFDAFVWAMEKEGVKDLPMVVTETGWP 272

Query: 260 TCGNANIATTSLAAKFNQNFKRHIVSGKGTPMKQNVYMEGFVHSIFNENQKPEGEPRCYG 319
           + GN N+ T  +A+ +N NF +H+ SGKGTP +    + GF+ + FNENQKP G  + +G
Sbjct: 273 SAGNGNLTTPDIASIYNTNFVKHVESGKGTPKRPKSGISGFLFATFNENQKPAGTEQNFG 332

Query: 320 IFN-VDSTPIYPLF 329
           ++N  D  PIY +F
Sbjct: 333 LYNPTDMKPIYKMF 344

[Close]

BLAST of Spg022100 vs. TAIR 10
Match: AT1G33220.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 204.5 bits (519), Expect = 1.3e-52
Identity = 118/313 (37.70%), Postives = 177/313 (56.55%), Query Frame = 0


Query: 20  LGAYYGLEANNLPPPWKVVQLCLKHNIRRVQLEEPNLDVFQAFRG-TGIDLSFSIPNDML 79
           +G  YGL  +N P P  V+ L     I ++++ +P  +V  A RG   I+++  + +  L
Sbjct: 34  IGLNYGLLGDNFPTPSNVLNLYKSIGITKIRIFDPKTEVLNALRGHRNIEVTVGVRDQDL 93

Query: 80  PNMATNNSAVEEWFNTYVKPFTDSFTINYIVVGDRALPG-LDDSILPVMKSLQNLLNANY 139
             ++ N  AV+ WF T ++P+     I +I VG+  +PG +   +LPVM+SL  L+ +  
Sbjct: 94  GALSANEEAVKGWFATNIEPYLADVNIAFITVGNEVIPGPIGPQVLPVMQSLTILVKSMN 153

Query: 140 LGQVKVTTFVGYKSVLSVKDLPSSSTFSPNIAETMRGILQFLSGEGSPLMISIDPYQKYF 199
           L  + ++T V   S L     PS+  F+    E +  +L+ LS   +P++++I PY  Y 
Sbjct: 154 L-PISISTVVA-MSNLEQSYPPSAGEFTSQAREQLVPVLKLLSQTSTPILVNIYPYFPYA 213

Query: 200 YSGT-ISLDYATFG-KATPVHDGTLTYYNLFDEMVDAFYVAIDKAKVGNVAIAVGQIGWP 259
              T I LDYATF  KAT V DG L Y N+FD + DAF  A++K  V ++ + V + GWP
Sbjct: 214 SDPTNIPLDYATFNTKATVVQDGPLGYSNMFDAIFDAFVWAMEKEGVKDLPMVVSETGWP 273

Query: 260 TCGNANIATTSLAAKFNQNFKRHIVSGKGTPMKQNVYMEGFVHSIFNENQKPEGEPRCYG 319
           + GN N+ T  +A  +N+NF +HIVSGKGTP + N  M+GF+ + FNENQKP G      
Sbjct: 274 SAGNGNLTTPDIAGTYNRNFVKHIVSGKGTPKRPNNGMDGFLFATFNENQKPAG------ 333

Query: 320 IFNVDSTPIYPLF 329
               D  PIY LF
Sbjct: 334 ---TDMKPIYKLF 335

[Close]

BLAST of Spg022100 vs. TAIR 10
Match: AT5G20560.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 195.3 bits (495), Expect = 7.8e-50
Identity = 106/307 (34.53%), Postives = 176/307 (57.33%), Query Frame = 0


Query: 19  LLGAYYGLEANNLPPPWKVVQLCLKHNIRRVQLEEPNLDVFQAFRGT-GIDLSFSIPNDM 78
           ++G  YGL  +NLP P  V++     N+ ++++ EPN DV  A RG   I ++  I N+ 
Sbjct: 33  VVGLNYGLLGDNLPSPSNVIKFYKSQNVAKIRIFEPNKDVLNALRGNRDIGVTVGIKNED 92

Query: 79  LPNMATNNSAVEEWFNTYVKPFTDSFTINYIVVGDRALPG--LDDSILPVMKSLQNLLNA 138
           L  +A N  AV+ WF+T + P+     I +I VG++A+PG      +LPV++SL +L+ +
Sbjct: 93  LEALAANKDAVKSWFSTNIDPYIADVNITFITVGNQAIPGDKHGPHVLPVIQSLTDLVKS 152

Query: 139 NYLGQVKVTTFVGYKSVLSVKDLPSSSTFSPNIAETMRGILQFLSGEGSPLMISIDPYQK 198
             L Q+ ++T V   S+  +K  PS+   +P   + +  +L+ LS   +P+ ++I PY  
Sbjct: 153 RNL-QISISTTVTTTSLARLKP-PSAGVLTPQARQQLVPVLRLLSQTSTPIFVNIYPY-- 212

Query: 199 YFYSG---TISLDYATFGK-ATPVHDGTLTYYNLFDEMVDAFYVAIDKAKVGNVAIAVGQ 258
           YF++     + L+YA F      V DG L Y NLFD + DAF  A++K  V  + + V +
Sbjct: 213 YFHASDPKNVPLEYANFNNDQIVVKDGALKYSNLFDAIFDAFLWAMEKEGVKGLPLVVSE 272

Query: 259 IGWPTCGNANIATTSLAAKFNQNFKRHIVSGKGTPMKQNVYMEGFVHSIFNENQKPEGEP 318
            GWP+ GN  + T +L   +  NF +H+ SGKGTP + N  ++ ++   +NENQKP G  
Sbjct: 273 TGWPSAGNGGMTTPALQYTYIGNFVKHVASGKGTPKRPNSRIDAYIFETYNENQKPVGIY 332

The following BLAST results are available for this feature:

BLAST of Spg022100 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6590489.1	4.5e-124	67.88	putative glucan endo-1,3-beta-glucosidase BG5, partial [Cucurbita argyrosperma s...	[more]
XP_022136187.1	7.6e-124	66.67	probable glucan endo-1,3-beta-glucosidase BG5 [Momordica charantia]	[more]
XP_022968751.1	3.0e-120	65.76	probable glucan endo-1,3-beta-glucosidase BG4 [Cucurbita maxima]	[more]
XP_038880685.1	1.9e-119	64.55	probable glucan endo-1,3-beta-glucosidase BG5 [Benincasa hispida]	[more]
TYK10032.1	1.1e-117	64.26	lichenase-2-like [Cucumis melo var. makuwa]	[more]

BLAST of Spg022100 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49353	1.0e-54	37.90	Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN...	[more]
O49352	3.0e-54	37.58	Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN...	[more]
O22317	3.3e-53	37.38	Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2	[more]
P12257	3.9e-46	34.84	Lichenase-2 (Fragment) OS=Hordeum vulgare OX=4513 PE=1 SV=1	[more]
P07979	3.3e-45	35.28	Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3	[more]

BLAST of Spg022100 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C4V7	3.7e-124	66.67	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111007943 ...	[more]
A0A6J1HVR6	1.5e-120	65.76	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111467895 PE=...	[more]
A0A5D3CFE1	5.2e-118	64.26	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A5A7UQE4	1.2e-117	64.26	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A5A7UIV1	7.0e-115	63.50	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]

BLAST of Spg022100 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20340.1	7.3e-56	37.90	beta-1,3-glucanase 5	[more]
AT5G20330.1	2.1e-55	37.58	beta-1,3-glucanase 4	[more]
AT5G20390.1	8.9e-54	36.31	Glycosyl hydrolase superfamily protein	[more]
AT1G33220.1	1.3e-52	37.70	Glycosyl hydrolase superfamily protein	[more]
AT5G20560.1	7.8e-50	34.53	Glycosyl hydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 19..328 e-value: 5.7E-90 score: 303.5
None	No IPR available	PANTHER	PTHR32227:SF305	GLYCOSYL HYDROLASE SUPERFAMILY PROTEIN-RELATED	coord: 14..326
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 20..326 e-value: 2.8E-60 score: 204.6
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 14..326
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 21..327

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg022100.1	Spg022100.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0046658	anchored component of plasma membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds