Homology
BLAST of Spg022055 vs. NCBI nr
Match:
XP_038879640.1 (isochorismate synthase 2, chloroplastic isoform X1 [Benincasa hispida])
HSP 1 Score: 915.2 bits (2364), Expect = 2.8e-262
Identity = 472/570 (82.81%), Postives = 498/570 (87.37%), Query Frame = 0
Query: 1 MSISKMAVSSTQNSATGECVWFRQHVSWRRRIPAVVAWPRRWRRW-GGVVCQWPAMDGCR 60
MSISKM VS +QNSAT +CV F Q VS RRIPA V WP R RRW G VCQ AMDGC
Sbjct: 1 MSISKMTVSRSQNSATRQCVGFGQRVSRCRRIPAAVPWPGRGRRWVVGEVCQSAAMDGCG 60
Query: 61 GDERGAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGIIRLQVPIQQHVQAI 120
GD G VGTIETR+FPAV+TAALA ESLR VAKLK EAP ++SGIIRLQVPIQQ +QAI
Sbjct: 61 GDGGGTVGTIETRTFPAVQTAALATESLRRGVAKLKLEAPALTSGIIRLQVPIQQRIQAI 120
Query: 121 DWLCSQPHLLPRTFFSG--RSRAIDPHPLSADNTADVRHPFNDHHLVGVAGVGSAVVFCR 180
DWL SQPHLLPRTFFSG RSRAID SA NT++ HPFN HHL+G AGVGSAVVF R
Sbjct: 121 DWLSSQPHLLPRTFFSGRSRSRAID----SAHNTSNGSHPFNHHHLLGAAGVGSAVVFHR 180
Query: 181 LHRFSNHDWASIKRFLSKKCPLIRAYGGIRFDSRANISSEWEPFGSFYFMVPQVEFDEFE 240
LH FS +DWASIKRFLSKKCPLIRAYG IRFDSRANIS EWEPFGSFYFMVPQVEFDEFE
Sbjct: 181 LHCFSYNDWASIKRFLSKKCPLIRAYGVIRFDSRANISPEWEPFGSFYFMVPQVEFDEFE 240
Query: 241 DNSMLAATVVWDHACSWTWENAVESLQSTIEQVSSNNVNLQRDLPHISIFSHNHVPSKTF 300
+NSMLAATV WDHACSWTWENAVESLQST+EQVSSNNVNLQ+DLP I I S+ HVPSKTF
Sbjct: 241 ENSMLAATVAWDHACSWTWENAVESLQSTMEQVSSNNVNLQKDLPLIPIHSYTHVPSKTF 300
Query: 301 WDRAIDRALQ-----------VVLARSTRIVTSVDVDPVAWVACLQKEGKNSYQFCVQPY 360
WDRA+DRALQ VVLARSTRIVTSVD+DPV WVACLQKEGKNSYQFCVQP
Sbjct: 301 WDRAVDRALQEIDRSNSELTKVVLARSTRIVTSVDIDPVTWVACLQKEGKNSYQFCVQPS 360
Query: 361 NAPAFIGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIEHDLFSNPKDDLEFSIVR 420
NAPAFIG+TPERLFHRE L IKS+ALAATRARGRS IED+KIEHDLFSNPKDDLEFSIVR
Sbjct: 361 NAPAFIGNTPERLFHREGLGIKSDALAATRARGRSPIEDIKIEHDLFSNPKDDLEFSIVR 420
Query: 421 ETIKRKLEA-----IVKPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCG 480
ETIK+KLEA +V+PHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCG
Sbjct: 421 ETIKKKLEAVCDAVVVEPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCG 480
Query: 481 LPTEEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVQRGLGALIYAGTGIVK 540
LPT EARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALV+RGLGALIYAGTGIVK
Sbjct: 481 LPTNEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVKRGLGALIYAGTGIVK 540
Query: 541 GSNPSSEWDELDLKISQVTRSLKLEVAQPP 552
GSNPSSEWDEL+LKISQ+TRSLKLEVAQ P
Sbjct: 541 GSNPSSEWDELELKISQITRSLKLEVAQAP 566
BLAST of Spg022055 vs. NCBI nr
Match:
XP_038879641.1 (isochorismate synthase 2, chloroplastic isoform X2 [Benincasa hispida])
HSP 1 Score: 888.6 bits (2295), Expect = 2.8e-254
Identity = 463/570 (81.23%), Postives = 489/570 (85.79%), Query Frame = 0
Query: 1 MSISKMAVSSTQNSATGECVWFRQHVSWRRRIPAVVAWPRRWRRW-GGVVCQWPAMDGCR 60
MSISKM VS +QNSAT +CV F Q VS RRIPA V WP R RRW G VCQ AMDGC
Sbjct: 1 MSISKMTVSRSQNSATRQCVGFGQRVSRCRRIPAAVPWPGRGRRWVVGEVCQSAAMDGCG 60
Query: 61 GDERGAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGIIRLQVPIQQHVQAI 120
GD G VGTIETR+FPAV+TAALA ESLR VAKLK EAP ++SGIIRLQVPIQQ +QAI
Sbjct: 61 GDGGGTVGTIETRTFPAVQTAALATESLRRGVAKLKLEAPALTSGIIRLQVPIQQRIQAI 120
Query: 121 DWLCSQPHLLPRTFFSG--RSRAIDPHPLSADNTADVRHPFNDHHLVGVAGVGSAVVFCR 180
DWL SQPHLLPRTFFSG RSRAID SA NT++ HPFN HHL+G AGVGSAVVF R
Sbjct: 121 DWLSSQPHLLPRTFFSGRSRSRAID----SAHNTSNGSHPFNHHHLLGAAGVGSAVVFHR 180
Query: 181 LHRFSNHDWASIKRFLSKKCPLIRAYGGIRFDSRANISSEWEPFGSFYFMVPQVEFDEFE 240
LH FS +DWASIKRFLSKKCPLIRAYG IRFDSRANIS EWEPFGSFYFMVPQVEFDEFE
Sbjct: 181 LHCFSYNDWASIKRFLSKKCPLIRAYGVIRFDSRANISPEWEPFGSFYFMVPQVEFDEFE 240
Query: 241 DNSMLAATVVWDHACSWTWENAVESLQSTIEQVSSNNVNLQRDLPHISIFSHNHVPSKTF 300
+NSMLAATV WDHACSWTWENAVESLQST+EQVSSNNVNLQ+DLP I I S+ HVPSKTF
Sbjct: 241 ENSMLAATVAWDHACSWTWENAVESLQSTMEQVSSNNVNLQKDLPLIPIHSYTHVPSKTF 300
Query: 301 WDRAIDRALQ-----------VVLARSTRIVTSVDVDPVAWVACLQKEGKNSYQFCVQPY 360
WDRA+DRALQ VVLARSTRIVTSVD+DPV WVACLQKEGKNSYQFCVQP
Sbjct: 301 WDRAVDRALQEIDRSNSELTKVVLARSTRIVTSVDIDPVTWVACLQKEGKNSYQFCVQPS 360
Query: 361 NAPAFIGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIEHDLFSNPKDDLEFSIVR 420
NAPAFIG+TPERLFHRE L IKS+ALAATRARGRS IED+KIEHDLFSNPKDDLEFSIVR
Sbjct: 361 NAPAFIGNTPERLFHREGLGIKSDALAATRARGRSPIEDIKIEHDLFSNPKDDLEFSIVR 420
Query: 421 ETIKRKLEA-----IVKPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCG 480
ETIK+KLEA +V+PHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCG
Sbjct: 421 ETIKKKLEAVCDAVVVEPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCG 480
Query: 481 LPTEEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVQRGLGALIYAGTGIVK 540
LPT EARDLIAET GPVGWFGGGESEFAVGIRSALV+RGLGALIYAGTGIVK
Sbjct: 481 LPTNEARDLIAET---------GPVGWFGGGESEFAVGIRSALVKRGLGALIYAGTGIVK 540
Query: 541 GSNPSSEWDELDLKISQVTRSLKLEVAQPP 552
GSNPSSEWDEL+LKISQ+TRSLKLEVAQ P
Sbjct: 541 GSNPSSEWDELELKISQITRSLKLEVAQAP 557
BLAST of Spg022055 vs. NCBI nr
Match:
XP_022932292.1 (isochorismate synthase 2, chloroplastic-like isoform X1 [Cucurbita moschata])
HSP 1 Score: 875.2 bits (2260), Expect = 3.2e-250
Identity = 451/566 (79.68%), Postives = 483/566 (85.34%), Query Frame = 0
Query: 1 MSISKMAVSSTQNSATGECVWFRQHVSWRRRIPAVVAWPRRW-RRWGGVVCQWPAMDGCR 60
MSI A+SST S TG CV F QH+S RRIP +V +P RW RRWGGVVCQ PAMDGCR
Sbjct: 1 MSIPNKAISST--SPTGPCVGFAQHLSPGRRIPTLVIFPGRWRRRWGGVVCQSPAMDGCR 60
Query: 61 GDERGAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGIIRLQVPIQQHVQAI 120
D GAV T+ETR+FPAV T LA E+LRG VAKLKSE P SSGIIRLQ+PIQ+H++AI
Sbjct: 61 ADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLPIQRHIKAI 120
Query: 121 DWLCSQPHLLPRTFFSGRSRAIDPHPLSADNTADVRHPFNDHH-LVGVAGVGSAVVFCRL 180
DWLCSQP LLPR FFSGRSRAID PL A ++ N HH L VAGVGSAVVFCR
Sbjct: 121 DWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSS-------NAHHRLASVAGVGSAVVFCRH 180
Query: 181 HRFSNHDWASIKRFLSKKCPLIRAYGGIRFDSRANISSEWEPFGSFYFMVPQVEFDEFED 240
H+FSN DWASIKR LSKKCPLIRAYGGIRFDSRANIS EWEPFGSFYFMVPQVEFDEFED
Sbjct: 181 HQFSNSDWASIKRLLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFED 240
Query: 241 NSMLAATVVWDHACSWTWENAVESLQSTIEQVSSNNVNLQRDLPHISIFSHNHVPSKTFW 300
+S+LAATV WDHACSWTWENAVESLQSTIEQVSS+ VN+QRDLP ISI SHNHVP+KTFW
Sbjct: 241 DSILAATVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFW 300
Query: 301 DRAIDRALQ-----------VVLARSTRIVTSVDVDPVAWVACLQKEGKNSYQFCVQPYN 360
D AI+RALQ VVLARSTRIVTSVDVDP+ WVACLQKEGKNSYQFCVQP N
Sbjct: 301 DHAINRALQVIDQCDSDLTKVVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSN 360
Query: 361 APAFIGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIEHDLFSNPKDDLEFSIVRE 420
APAFIG+TPERLFHRE LSI EALAATRARGRSAIEDLKIE DL SNPKDDLEFSIVRE
Sbjct: 361 APAFIGNTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLSNPKDDLEFSIVRE 420
Query: 421 TIKRKLEA-----IVKPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCGL 480
IKRKLEA +V+PHKSVRKLPRIQHLYAQL GRLR+++DEFEILSTLHPTPAVCG
Sbjct: 421 AIKRKLEAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGF 480
Query: 481 PTEEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVQRGLGALIYAGTGIVKG 540
PTEEARDLIAETE+FDRGMYAGPVGWFGG ESEFAVGIRSALV+ GLGAL+YAGTGIVKG
Sbjct: 481 PTEEARDLIAETELFDRGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVKG 540
Query: 541 SNPSSEWDELDLKISQVTRSLKLEVA 549
SNPS EWDEL+LKISQVTRSLKLE+A
Sbjct: 541 SNPSLEWDELELKISQVTRSLKLELA 557
BLAST of Spg022055 vs. NCBI nr
Match:
XP_022972451.1 (isochorismate synthase 2, chloroplastic isoform X1 [Cucurbita maxima])
HSP 1 Score: 874.4 bits (2258), Expect = 5.5e-250
Identity = 450/567 (79.37%), Postives = 484/567 (85.36%), Query Frame = 0
Query: 1 MSISKMAVSST-QNSATGECVWFRQHVSWRRRIPAVVAWPRRW-RRWGGVVCQWPAMDGC 60
MSI A+SST Q S TG CV F QH+S RRIP V WP RW RRWGGVVCQ PAMDGC
Sbjct: 1 MSIPNKAISSTSQTSPTGPCVGFAQHLSPGRRIPTRVIWPGRWRRRWGGVVCQSPAMDGC 60
Query: 61 RGDERGAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGIIRLQVPIQQHVQA 120
R D GAV T+ETR+FPAV T LA E+LRG VAKLKSE P SSGIIRLQ+PIQQH++A
Sbjct: 61 RADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLPIQQHIKA 120
Query: 121 IDWLCSQPHLLPRTFFSGRSRAIDPHPLSADNTADVRHPFNDHH-LVGVAGVGSAVVFCR 180
I WLCSQP LLPR FFSGRSRAID PL + ++ N HH L VAGVGSAVVFCR
Sbjct: 121 IHWLCSQPLLLPRIFFSGRSRAIDSDPLPSVSS-------NAHHRLASVAGVGSAVVFCR 180
Query: 181 LHRFSNHDWASIKRFLSKKCPLIRAYGGIRFDSRANISSEWEPFGSFYFMVPQVEFDEFE 240
H+FSN DWASIKRFLSKKCPLIRA+GGIRFDSRANIS EWEPFGSFYFMVPQVEFDEFE
Sbjct: 181 HHQFSNSDWASIKRFLSKKCPLIRAFGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFE 240
Query: 241 DNSMLAATVVWDHACSWTWENAVESLQSTIEQVSSNNVNLQRDLPHISIFSHNHVPSKTF 300
D+S+LAATV WDHACSWTWENAVESLQSTIEQVSS+NVN+QRDLP ISI SHNHVP+KTF
Sbjct: 241 DDSILAATVAWDHACSWTWENAVESLQSTIEQVSSDNVNVQRDLPQISILSHNHVPTKTF 300
Query: 301 WDRAIDRALQ-----------VVLARSTRIVTSVDVDPVAWVACLQKEGKNSYQFCVQPY 360
WDRAI+RALQ VVLARSTRIVTSV+VDPV WVACLQKEG++SYQFCVQP
Sbjct: 301 WDRAINRALQAIDQCDSDLTKVVLARSTRIVTSVNVDPVTWVACLQKEGESSYQFCVQPA 360
Query: 361 NAPAFIGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIEHDLFSNPKDDLEFSIVR 420
NAPAFIG+TPERLFHRE LSI EALAATRARG+SAIEDLKIE DL SNPKDDLEFSIVR
Sbjct: 361 NAPAFIGNTPERLFHREGLSIICEALAATRARGKSAIEDLKIEQDLLSNPKDDLEFSIVR 420
Query: 421 ETIKRKLEA-----IVKPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCG 480
E IKRKLEA +V+PHKSVRKLPRIQHLYAQL GRLR+++DEFEILSTLHPTPAVCG
Sbjct: 421 EAIKRKLEAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCG 480
Query: 481 LPTEEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVQRGLGALIYAGTGIVK 540
PTEEARDLIAETE+FDRGMYAGPVGWFGG ESEFAVGIRSALV+ G GAL+YAGTGIVK
Sbjct: 481 FPTEEARDLIAETELFDRGMYAGPVGWFGGEESEFAVGIRSALVETGHGALVYAGTGIVK 540
Query: 541 GSNPSSEWDELDLKISQVTRSLKLEVA 549
GSNPS EWDEL+LKISQVTRSLKLE+A
Sbjct: 541 GSNPSLEWDELELKISQVTRSLKLELA 560
BLAST of Spg022055 vs. NCBI nr
Match:
XP_023530897.1 (isochorismate synthase 2, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 866.3 bits (2237), Expect = 1.5e-247
Identity = 447/567 (78.84%), Postives = 480/567 (84.66%), Query Frame = 0
Query: 1 MSISKMAVSST-QNSATGECVWFRQHVSWRRRIPAVVAWPRRW-RRWGGVVCQWPAMDGC 60
MSI A+SST Q S TG CV F QH+S RRIP +V P RW RRWG VVCQ PAMDGC
Sbjct: 4 MSIPSKAISSTSQTSPTGPCVGFAQHLSPGRRIPTLVISPGRWRRRWGRVVCQSPAMDGC 63
Query: 61 RGDERGAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGIIRLQVPIQQHVQA 120
R D GAV T+ETR+FPAV T LA E+LRG VAKLKSE P SSGIIRLQ+PI +H++A
Sbjct: 64 RADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLPIHRHIKA 123
Query: 121 IDWLCSQPHLLPRTFFSGRSRAIDPHPLSADNTADVRHPFNDHHLV-GVAGVGSAVVFCR 180
IDWLCSQP LLPR FFSGRSRAID L + ++ N HH V VAGVGSAVVFCR
Sbjct: 124 IDWLCSQPLLLPRIFFSGRSRAIDSDSLPSVSS-------NAHHRVASVAGVGSAVVFCR 183
Query: 181 LHRFSNHDWASIKRFLSKKCPLIRAYGGIRFDSRANISSEWEPFGSFYFMVPQVEFDEFE 240
H+FSN DWASIKRFLSKKCPLIRAYGGIRFDSR NIS EWEPFGSFYFMVPQVEFDEFE
Sbjct: 184 HHQFSNSDWASIKRFLSKKCPLIRAYGGIRFDSRPNISPEWEPFGSFYFMVPQVEFDEFE 243
Query: 241 DNSMLAATVVWDHACSWTWENAVESLQSTIEQVSSNNVNLQRDLPHISIFSHNHVPSKTF 300
D+S+LAATV WDHACSWTWENAVESLQST+EQVSS+NVN+QRDLP ISI SHNHVP+KTF
Sbjct: 244 DDSILAATVAWDHACSWTWENAVESLQSTVEQVSSDNVNVQRDLPQISILSHNHVPTKTF 303
Query: 301 WDRAIDRALQ-----------VVLARSTRIVTSVDVDPVAWVACLQKEGKNSYQFCVQPY 360
WD AI+RALQ VVLARSTRIVTSVDVDPV WVACLQKEGKNSYQFCVQP
Sbjct: 304 WDSAINRALQVIDQCDSDLTKVVLARSTRIVTSVDVDPVTWVACLQKEGKNSYQFCVQPS 363
Query: 361 NAPAFIGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIEHDLFSNPKDDLEFSIVR 420
NAPAFIG+TPERLFHRE LSI EALAATRARG+SAIEDL+IE DL SNPKDDLEFSIVR
Sbjct: 364 NAPAFIGNTPERLFHREGLSIICEALAATRARGKSAIEDLRIEQDLLSNPKDDLEFSIVR 423
Query: 421 ETIKRKLEA-----IVKPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCG 480
E IKRKLEA +V+PHKSVRKLPRIQHLYAQL GRLR+ +DEFEILSTLHPTPAVCG
Sbjct: 424 EAIKRKLEAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTNDDEFEILSTLHPTPAVCG 483
Query: 481 LPTEEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVQRGLGALIYAGTGIVK 540
PTEEARDLIAETE+FDRGMYAGPVGWFGG ESEFAVGIRSALV+ GLGAL+YAGTGIVK
Sbjct: 484 FPTEEARDLIAETELFDRGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVK 543
Query: 541 GSNPSSEWDELDLKISQVTRSLKLEVA 549
GSNPS EWDEL+LKISQVTRSLKLE+A
Sbjct: 544 GSNPSLEWDELELKISQVTRSLKLELA 563
BLAST of Spg022055 vs. ExPASy Swiss-Prot
Match:
Q9M9V6 (Isochorismate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ICS2 PE=2 SV=2)
HSP 1 Score: 597.0 bits (1538), Expect = 2.2e-169
Identity = 310/517 (59.96%), Postives = 386/517 (74.66%), Query Frame = 0
Query: 54 AMDGCRGDERGAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGIIRLQVPIQ 113
+M+GC D + +GT+ETR+ V + A A E L V+ LKS+ PP SSGI+RLQVPI+
Sbjct: 50 SMNGCEADHKAPLGTVETRTLSTVPSPAAATERLITAVSDLKSQPPPFSSGIVRLQVPIE 109
Query: 114 QHVQAIDWLCSQPHLLPRTFFSGRSRAIDPHPL---SADNTADVRHPFNDHHLVGVAGVG 173
Q + AIDWL +Q +LPR+FFS RS + P L S+DN + +DH+ V VAG+G
Sbjct: 110 QKIGAIDWLHAQNEILPRSFFSRRSDSGRPDLLQDFSSDNGS------SDHNPVSVAGIG 169
Query: 174 SAVVFCRLHRFSNHDWASIKRFLSKKCPLIRAYGGIRFDSRANISSEWEPFGSFYFMVPQ 233
SAV F L FS+ DW SI+RFLS K PLIRAYGG+RFD I+ EWE FGSFYF VPQ
Sbjct: 170 SAVFFRDLDPFSHDDWRSIRRFLSSKSPLIRAYGGLRFDPTGKIAVEWEHFGSFYFTVPQ 229
Query: 234 VEFDEFEDNSMLAATVVWDHACSWTWENAVESLQSTIEQVSSNNVNLQRDLPHISIFSHN 293
VEFDEF +SMLAATV WD+ SWT ENA+E+LQ T+ QVSS + L+R+ + + S N
Sbjct: 230 VEFDEFGGSSMLAATVAWDNELSWTLENAIEALQETMLQVSSVIMRLRRESLGVIVVSKN 289
Query: 294 HVPSKTFWDRAIDRALQ-----------VVLARSTRIVTSVDVDPVAWVACLQKEGKNSY 353
HVPS+ + A++ AL+ VVLARS+RI+T D+DP+AW+A LQ EG+++Y
Sbjct: 290 HVPSEGAYYPAVNNALEIIKDKHSPLSKVVLARSSRIITDTDIDPIAWLARLQCEGQDAY 349
Query: 354 QFCVQPYNAPAFIGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIEHDLFSNPKDD 413
QFC+QP APAFIG+TPERLFHR+ L + SEALAATR RG S + +++IE DL ++PKDD
Sbjct: 350 QFCLQPPGAPAFIGNTPERLFHRKHLGVCSEALAATRPRGDSKVREMEIERDLLTSPKDD 409
Query: 414 LEFSIVRETIKRKLEAI-----VKPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLH 473
LEFSIVRE I+ KL+ I VKPHKSVRKL R+QHLY+QL G+L+ E+DEF IL+ LH
Sbjct: 410 LEFSIVRENIREKLKTICDRVVVKPHKSVRKLARVQHLYSQLAGQLKREDDEFNILTALH 469
Query: 474 PTPAVCGLPTEEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVQRGLGALIY 533
PTPAVCG P EEAR LI + E FDRGMYAGP+G+FGGGESEF+VGIRSALV++GLGALIY
Sbjct: 470 PTPAVCGCPVEEARLLIKQIESFDRGMYAGPIGFFGGGESEFSVGIRSALVEKGLGALIY 529
Query: 534 AGTGIVKGSNPSSEWDELDLKISQVTRSLKLEVAQPP 552
AGTGIV GSNPSSEW+EL+LKISQ T+SL+ E A P
Sbjct: 530 AGTGIVSGSNPSSEWNELELKISQFTKSLEHESALQP 560
BLAST of Spg022055 vs. ExPASy Swiss-Prot
Match:
Q9ZPC0 (Isochorismate synthase, chloroplastic OS=Catharanthus roseus OX=4058 PE=1 SV=1)
HSP 1 Score: 574.7 bits (1480), Expect = 1.2e-162
Identity = 294/522 (56.32%), Postives = 384/522 (73.56%), Query Frame = 0
Query: 54 AMDGCRGDERGAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGIIRLQVPIQ 113
+M+GC GD R VGTIETR+ PAV T ALA E L VA LKS P SGIIRL+VPI+
Sbjct: 54 SMNGCNGDPRAPVGTIETRTLPAVSTPALAMERLSSAVANLKSTLPSAQSGIIRLEVPIE 113
Query: 114 QHVQAIDWLCS--QPHLLPRTFFSGRSRAIDPHPLSADNT-----------ADVRHPFND 173
+H++A+DWL S Q +LLPR +FSGRS+ S D T + +D
Sbjct: 114 EHIEALDWLHSQDQKNLLPRCYFSGRSQVTFSDFTSNDLTNRNGSAANGHLQRISTSSDD 173
Query: 174 HHLVGVAGVGSAVVFCRLHRFSNHDWASIKRFLSKKCPLIRAYGGIRFDSRANISSEWEP 233
+LV VAGVGSAV+F + FS DW SIKRFLSK CPLIRAYG IRFD+R +I+ EW+
Sbjct: 174 KNLVSVAGVGSAVLFRSPNPFSFDDWLSIKRFLSKNCPLIRAYGAIRFDARPHIAPEWKA 233
Query: 234 FGSFYFMVPQVEFDEFEDNSMLAATVVWDHACSWTWENAVESLQSTIEQVSSNNVNLQRD 293
FGSFYF+VPQVEFDE +SM+AATV WD+A S T++ A+ +LQ+T+EQVSS L++D
Sbjct: 234 FGSFYFVVPQVEFDELHGSSMIAATVAWDNALSLTYQQAIVALQTTMEQVSSTVSKLRQD 293
Query: 294 LPHISIFSHNHVPSKTFWDRAIDRALQ-----------VVLARSTRIVTSVDVDPVAWVA 353
+ H S+ S ++P +T WD ++R L+ VVLAR ++++T+ D+DP+AW++
Sbjct: 294 VSHTSLVSKANIPDRTSWDLTLNRVLEEIGNKYSPLTKVVLARRSQVITTSDIDPLAWLS 353
Query: 354 CLQKEGKNSYQFCVQPYNAPAFIGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIE 413
+ +GK++YQFC+QP+ APAFIG+TPE+LF R+ L++ SEALAATRARG S DL++
Sbjct: 354 SFKADGKDAYQFCLQPHEAPAFIGNTPEQLFGRDQLTVFSEALAATRARGESDSLDLQMA 413
Query: 414 HDLFSNPKDDLEFSIVRETIKRKLEAI-----VKPHKSVRKLPRIQHLYAQLTGRLRSEE 473
HDLFS+PKD+ EF+IVRE I++KL+AI +P KSVRKL RIQHLYA+ GRLRSE+
Sbjct: 414 HDLFSSPKDNHEFAIVRENIRQKLDAICTSVETEPMKSVRKLKRIQHLYARFAGRLRSED 473
Query: 474 DEFEILSTLHPTPAVCGLPTEEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSAL 533
DEF+ILS+LHPTPAVCG P E+AR IAE E+FDRG+YAGPVG+FGG +S+F+VGIRSAL
Sbjct: 474 DEFKILSSLHPTPAVCGFPMEDARKFIAENEMFDRGLYAGPVGFFGGAQSDFSVGIRSAL 533
Query: 534 VQRGLGALIYAGTGIVKGSNPSSEWDELDLKISQVTRSLKLE 547
+ + GALIYAG G+V+GS+P+ EW EL+LK SQ + +KLE
Sbjct: 534 IGKDAGALIYAGLGVVEGSDPALEWQELELKASQFMKLMKLE 575
BLAST of Spg022055 vs. ExPASy Swiss-Prot
Match:
Q9S7H8 (Isochorismate synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ICS1 PE=1 SV=2)
HSP 1 Score: 572.0 bits (1473), Expect = 7.6e-162
Identity = 299/514 (58.17%), Postives = 372/514 (72.37%), Query Frame = 0
Query: 54 AMDGCRGDERGAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGIIRLQVPIQ 113
+M+GC GD + +GT+ETR+ AV + A A E L V++LKS+ P SSG++RLQVPI
Sbjct: 47 SMNGCDGDFKTPLGTVETRTMTAVLSPAAATERLISAVSELKSQPPSFSSGVVRLQVPID 106
Query: 114 QHVQAIDWLCSQPHLLPRTFFSGRSRAIDPHPL-----SADNTADVRHPFNDHHLVGVAG 173
Q + AIDWL +Q + PR FFS RS P L N +D +LV VAG
Sbjct: 107 QQIGAIDWLQAQNEIQPRCFFSRRSDVGRPDLLLDLANENGNGNGNGTVSSDRNLVSVAG 166
Query: 174 VGSAVVFCRLHRFSNHDWASIKRFLSKKCPLIRAYGGIRFDSRANISSEWEPFGSFYFMV 233
+GSAV F L FS+ DW SI+RFLS PLIRAYGG+RFD I+ EWEPFG+FYF V
Sbjct: 167 IGSAVFFRDLDPFSHDDWRSIRRFLSSTSPLIRAYGGMRFDPNGKIAVEWEPFGAFYFSV 226
Query: 234 PQVEFDEFEDNSMLAATVVWDHACSWTWENAVESLQSTIEQVSSNNVNLQRDLPHISIFS 293
PQVEF+EF +SMLAAT+ WD SWT ENA+E+LQ T+ QVSS + L+ +S+ S
Sbjct: 227 PQVEFNEFGGSSMLAATIAWDDELSWTLENAIEALQETMLQVSSVVMKLRNRSLGVSVLS 286
Query: 294 HNHVPSKTFWDRAIDRALQ-----------VVLARSTRIVTSVDVDPVAWVACLQKEGKN 353
NHVP+K + A+++AL+ VVLAR++RI+T D+DP+AW+A LQ+EG +
Sbjct: 287 KNHVPTKGAYFPAVEKALEMINQKSSPLNKVVLARNSRIITDTDIDPIAWLAQLQREGHD 346
Query: 354 SYQFCVQPYNAPAFIGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIEHDLFSNPK 413
+YQFC+QP APAFIG+TPERLF R L + SEALAATR R S+ D++IE DL ++PK
Sbjct: 347 AYQFCLQPPGAPAFIGNTPERLFQRTQLGVCSEALAATRPRAASSARDMEIERDLLTSPK 406
Query: 414 DDLEFSIVRETIKRKLEAI-----VKPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILST 473
DDLEFSIVRE I+ KL I VKP K+VRKL R+QHLY+QL GRL E+DE++IL+
Sbjct: 407 DDLEFSIVRENIREKLNGICDRVVVKPQKTVRKLARVQHLYSQLAGRLTKEDDEYKILAA 466
Query: 474 LHPTPAVCGLPTEEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVQRGLGAL 533
LHPTPAVCGLP EEAR LI E E FDRGMYAGP+G+FGG ESEFAVGIRSALV++GLGAL
Sbjct: 467 LHPTPAVCGLPAEEARLLIKEIESFDRGMYAGPIGFFGGEESEFAVGIRSALVEKGLGAL 526
Query: 534 IYAGTGIVKGSNPSSEWDELDLKISQVTRSLKLE 547
IYAGTGIV GS+PSSEW+ELDLKISQ T+S++ E
Sbjct: 527 IYAGTGIVAGSDPSSEWNELDLKISQFTKSIEYE 560
BLAST of Spg022055 vs. ExPASy Swiss-Prot
Match:
P23973 (Isochorismate synthase MenF OS=Bacillus subtilis (strain 168) OX=224308 GN=menF PE=3 SV=2)
HSP 1 Score: 144.1 bits (362), Expect = 5.1e-33
Identity = 108/364 (29.67%), Postives = 172/364 (47.25%), Query Frame = 0
Query: 203 YGGIRFDSRANISSEWEPFGSFYFMVPQVEFDEFEDNSMLAATVVWDHACSWTWENAVES 262
+GG FD S+W+ F F VP + ML T W + E
Sbjct: 119 FGGFSFDPCEERGSQWDHFSEGDFFVPAL---------MLTMTAEGPFLTVNRWVSGGED 178
Query: 263 LQSTIEQVSSNNVNL------QRDLPHISIFSHNHVPSKTFWDRAIDRAL---------Q 322
++ +E + + Q D ++ + K W +AI+ A +
Sbjct: 179 AEAVLEGLKAFAAEFMVPDFKQEDQ---AVIAAAEELDKDDWLKAIETATSQIKEKQYDK 238
Query: 323 VVLARSTRIVTS--VDVDPVAWVACLQKEGKNSYQFCVQPYNAPAFIGSTPERLFHREAL 382
VVLAR + + ++PV + L + + SY F ++ F+G++PERL R+
Sbjct: 239 VVLARELLLTFDGPIQIEPV--LKTLLDDQQTSYVFAIE-QEGKTFVGASPERLIKRDGG 298
Query: 383 SIKSEALAATRARGRSAIEDLKIEHDLFSNPKDDLEFSIV----RETIKRKLEAIVKPHK 442
++ S LA + RG + +D +I +L ++ K+ LE IV + KP
Sbjct: 299 TVMSSCLAGSIKRGVNEEDDRRIGLELLNDEKNLLEHDIVVGMIHNAFVSSCSEVEKPDG 358
Query: 443 SV-RKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCGLPTEEARDLIAETEVFDRG 502
V K +QHL+ + G+LR F+++ LHPTPA+ G P E+A D+I E E RG
Sbjct: 359 PVLYKTKSVQHLFTPIVGQLRESASIFDLIEKLHPTPALGGSPQEKAVDVIREIEPMSRG 418
Query: 503 MYAGPVGWFGGGES-EFAVGIRSALVQRGLGALIYAGTGIVKGSNPSSEWDELDLKISQV 544
YA P+GW ++ EFAV IRS L++ G A ++AG GIV+ S P SE++E +K+ +
Sbjct: 419 WYAAPIGWIDSQDNGEFAVAIRSGLIE-GSTARLFAGCGIVEDSEPISEYEETQIKLKPM 466
BLAST of Spg022055 vs. ExPASy Swiss-Prot
Match:
P38051 (Isochorismate synthase MenF OS=Escherichia coli (strain K12) OX=83333 GN=menF PE=1 SV=4)
HSP 1 Score: 138.3 bits (347), Expect = 2.8e-31
Identity = 95/279 (34.05%), Postives = 147/279 (52.69%), Query Frame = 0
Query: 283 HISIFSHNHVPSKTFWDRAIDRAL---------QVVLARSTRIVTSVDVDPVAWVACLQK 342
H++ H P KT W + I+ A +VVLAR+T + + V+ A +A ++
Sbjct: 157 HLTTTREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRR 216
Query: 343 EGKNSYQFCVQPYNAPAFIGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIEHDLF 402
N Y F + AF+GS+PERL+ R ++++EALA T A + ++ L
Sbjct: 217 LNLNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM 276
Query: 403 SNPKDDLEFSIVRETIKRKLEA------IVKPHKSVRKLPRIQHLYAQLTGRLRSEEDEF 462
++ K+ E +V E I ++L+A ++ P V +L ++QHL + L ++ D+
Sbjct: 277 ADDKNQRENMLVVEDICQRLQADTQTLDVLPP--QVLRLRKVQHLRRCIWTSL-NKADDV 336
Query: 463 EILSTLHPTPAVCGLPTEEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVQR 522
L L PT AV GLP + AR IA E F R YAG G+ +SEF V +RSA +
Sbjct: 337 ICLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS- 396
Query: 523 GLGALIYAGTGIVKGSNPSSEWDELDLKISQVTRSLKLE 547
G +YAG GIV+GS+P EW E+D K + + L++E
Sbjct: 397 GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME 431
BLAST of Spg022055 vs. ExPASy TrEMBL
Match:
A0A6J1EW89 (Isochorismate synthase OS=Cucurbita moschata OX=3662 GN=LOC111438650 PE=3 SV=1)
HSP 1 Score: 875.2 bits (2260), Expect = 1.6e-250
Identity = 451/566 (79.68%), Postives = 483/566 (85.34%), Query Frame = 0
Query: 1 MSISKMAVSSTQNSATGECVWFRQHVSWRRRIPAVVAWPRRW-RRWGGVVCQWPAMDGCR 60
MSI A+SST S TG CV F QH+S RRIP +V +P RW RRWGGVVCQ PAMDGCR
Sbjct: 1 MSIPNKAISST--SPTGPCVGFAQHLSPGRRIPTLVIFPGRWRRRWGGVVCQSPAMDGCR 60
Query: 61 GDERGAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGIIRLQVPIQQHVQAI 120
D GAV T+ETR+FPAV T LA E+LRG VAKLKSE P SSGIIRLQ+PIQ+H++AI
Sbjct: 61 ADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLPIQRHIKAI 120
Query: 121 DWLCSQPHLLPRTFFSGRSRAIDPHPLSADNTADVRHPFNDHH-LVGVAGVGSAVVFCRL 180
DWLCSQP LLPR FFSGRSRAID PL A ++ N HH L VAGVGSAVVFCR
Sbjct: 121 DWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSS-------NAHHRLASVAGVGSAVVFCRH 180
Query: 181 HRFSNHDWASIKRFLSKKCPLIRAYGGIRFDSRANISSEWEPFGSFYFMVPQVEFDEFED 240
H+FSN DWASIKR LSKKCPLIRAYGGIRFDSRANIS EWEPFGSFYFMVPQVEFDEFED
Sbjct: 181 HQFSNSDWASIKRLLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFED 240
Query: 241 NSMLAATVVWDHACSWTWENAVESLQSTIEQVSSNNVNLQRDLPHISIFSHNHVPSKTFW 300
+S+LAATV WDHACSWTWENAVESLQSTIEQVSS+ VN+QRDLP ISI SHNHVP+KTFW
Sbjct: 241 DSILAATVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFW 300
Query: 301 DRAIDRALQ-----------VVLARSTRIVTSVDVDPVAWVACLQKEGKNSYQFCVQPYN 360
D AI+RALQ VVLARSTRIVTSVDVDP+ WVACLQKEGKNSYQFCVQP N
Sbjct: 301 DHAINRALQVIDQCDSDLTKVVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSN 360
Query: 361 APAFIGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIEHDLFSNPKDDLEFSIVRE 420
APAFIG+TPERLFHRE LSI EALAATRARGRSAIEDLKIE DL SNPKDDLEFSIVRE
Sbjct: 361 APAFIGNTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLSNPKDDLEFSIVRE 420
Query: 421 TIKRKLEA-----IVKPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCGL 480
IKRKLEA +V+PHKSVRKLPRIQHLYAQL GRLR+++DEFEILSTLHPTPAVCG
Sbjct: 421 AIKRKLEAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGF 480
Query: 481 PTEEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVQRGLGALIYAGTGIVKG 540
PTEEARDLIAETE+FDRGMYAGPVGWFGG ESEFAVGIRSALV+ GLGAL+YAGTGIVKG
Sbjct: 481 PTEEARDLIAETELFDRGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVKG 540
Query: 541 SNPSSEWDELDLKISQVTRSLKLEVA 549
SNPS EWDEL+LKISQVTRSLKLE+A
Sbjct: 541 SNPSLEWDELELKISQVTRSLKLELA 557
BLAST of Spg022055 vs. ExPASy TrEMBL
Match:
A0A6J1I605 (Isochorismate synthase OS=Cucurbita maxima OX=3661 GN=LOC111471007 PE=3 SV=1)
HSP 1 Score: 874.4 bits (2258), Expect = 2.6e-250
Identity = 450/567 (79.37%), Postives = 484/567 (85.36%), Query Frame = 0
Query: 1 MSISKMAVSST-QNSATGECVWFRQHVSWRRRIPAVVAWPRRW-RRWGGVVCQWPAMDGC 60
MSI A+SST Q S TG CV F QH+S RRIP V WP RW RRWGGVVCQ PAMDGC
Sbjct: 1 MSIPNKAISSTSQTSPTGPCVGFAQHLSPGRRIPTRVIWPGRWRRRWGGVVCQSPAMDGC 60
Query: 61 RGDERGAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGIIRLQVPIQQHVQA 120
R D GAV T+ETR+FPAV T LA E+LRG VAKLKSE P SSGIIRLQ+PIQQH++A
Sbjct: 61 RADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLPIQQHIKA 120
Query: 121 IDWLCSQPHLLPRTFFSGRSRAIDPHPLSADNTADVRHPFNDHH-LVGVAGVGSAVVFCR 180
I WLCSQP LLPR FFSGRSRAID PL + ++ N HH L VAGVGSAVVFCR
Sbjct: 121 IHWLCSQPLLLPRIFFSGRSRAIDSDPLPSVSS-------NAHHRLASVAGVGSAVVFCR 180
Query: 181 LHRFSNHDWASIKRFLSKKCPLIRAYGGIRFDSRANISSEWEPFGSFYFMVPQVEFDEFE 240
H+FSN DWASIKRFLSKKCPLIRA+GGIRFDSRANIS EWEPFGSFYFMVPQVEFDEFE
Sbjct: 181 HHQFSNSDWASIKRFLSKKCPLIRAFGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFE 240
Query: 241 DNSMLAATVVWDHACSWTWENAVESLQSTIEQVSSNNVNLQRDLPHISIFSHNHVPSKTF 300
D+S+LAATV WDHACSWTWENAVESLQSTIEQVSS+NVN+QRDLP ISI SHNHVP+KTF
Sbjct: 241 DDSILAATVAWDHACSWTWENAVESLQSTIEQVSSDNVNVQRDLPQISILSHNHVPTKTF 300
Query: 301 WDRAIDRALQ-----------VVLARSTRIVTSVDVDPVAWVACLQKEGKNSYQFCVQPY 360
WDRAI+RALQ VVLARSTRIVTSV+VDPV WVACLQKEG++SYQFCVQP
Sbjct: 301 WDRAINRALQAIDQCDSDLTKVVLARSTRIVTSVNVDPVTWVACLQKEGESSYQFCVQPA 360
Query: 361 NAPAFIGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIEHDLFSNPKDDLEFSIVR 420
NAPAFIG+TPERLFHRE LSI EALAATRARG+SAIEDLKIE DL SNPKDDLEFSIVR
Sbjct: 361 NAPAFIGNTPERLFHREGLSIICEALAATRARGKSAIEDLKIEQDLLSNPKDDLEFSIVR 420
Query: 421 ETIKRKLEA-----IVKPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCG 480
E IKRKLEA +V+PHKSVRKLPRIQHLYAQL GRLR+++DEFEILSTLHPTPAVCG
Sbjct: 421 EAIKRKLEAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCG 480
Query: 481 LPTEEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVQRGLGALIYAGTGIVK 540
PTEEARDLIAETE+FDRGMYAGPVGWFGG ESEFAVGIRSALV+ G GAL+YAGTGIVK
Sbjct: 481 FPTEEARDLIAETELFDRGMYAGPVGWFGGEESEFAVGIRSALVETGHGALVYAGTGIVK 540
Query: 541 GSNPSSEWDELDLKISQVTRSLKLEVA 549
GSNPS EWDEL+LKISQVTRSLKLE+A
Sbjct: 541 GSNPSLEWDELELKISQVTRSLKLELA 560
BLAST of Spg022055 vs. ExPASy TrEMBL
Match:
A0A0A0LS91 (Isochorismate synthase OS=Cucumis sativus OX=3659 GN=Csa_1G008580 PE=3 SV=1)
HSP 1 Score: 862.8 bits (2228), Expect = 8.0e-247
Identity = 444/565 (78.58%), Postives = 479/565 (84.78%), Query Frame = 0
Query: 8 VSSTQNSATG-ECVWFRQHVSWRRRIPAVVAWPRRWRRWGGVVCQWPAMDGCRGDER--- 67
+S NSATG C+ F Q +S RRI VAW R RRWGG VC AMDGC G +
Sbjct: 1 MSIANNSATGRHCLGFHQRLSRCRRISPAVAWRERGRRWGGEVCDSAAMDGCVGGSQEPG 60
Query: 68 GAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGIIRLQVPIQQHVQAIDWLC 127
G VG IETR+FPAV+TAALA E LR VA+LKSEAP +SGIIRLQVPIQQ +QAIDWL
Sbjct: 61 GTVGIIETRNFPAVQTAALATERLRRGVAELKSEAPAFTSGIIRLQVPIQQRIQAIDWLS 120
Query: 128 SQPHLLPRTFFSGRSRAIDPHPLSADNTADVRHPFN-DHHLVGVAGVGSAVVFCRLHRFS 187
SQPHLLPRTFFSGRSR P SA N ++ H FN HHL+ VAGVGSAVVF RLH FS
Sbjct: 121 SQPHLLPRTFFSGRSR---PTHSSAHNISNGSHSFNHHHHLLSVAGVGSAVVFRRLHCFS 180
Query: 188 NHDWASIKRFLSKKCPLIRAYGGIRFDSRANISSEWEPFGSFYFMVPQVEFDEFEDNSML 247
+DWASIKRFLSKKCPLIRAYG IRFDSRANIS EWEPFGSFYFMVPQVEFDEFE+NSML
Sbjct: 181 YNDWASIKRFLSKKCPLIRAYGVIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEENSML 240
Query: 248 AATVVWDHACSWTWENAVESLQSTIEQVSSNNVNLQRDLPHISIFSHNHVPSKTFWDRAI 307
AATV WDHACSWTWENA+ESLQSTIEQVSSNNVNLQ+DLP I I S+ HVPSKTFW+RA+
Sbjct: 241 AATVAWDHACSWTWENAIESLQSTIEQVSSNNVNLQKDLPQIPILSYTHVPSKTFWERAV 300
Query: 308 DRALQ-----------VVLARSTRIVTSVDVDPVAWVACLQKEGKNSYQFCVQPYNAPAF 367
DRALQ VVLARST+IVT VD+DPV WVACLQKEG NSYQFCVQP NAPAF
Sbjct: 301 DRALQVIDGSNSELTKVVLARSTQIVTGVDIDPVTWVACLQKEGNNSYQFCVQPSNAPAF 360
Query: 368 IGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIEHDLFSNPKDDLEFSIVRETIKR 427
IG+TPERLFHRE L IKS+ALAATRARGR+AIED+KIEHDLFSNPK+DLEFSIVRETIK+
Sbjct: 361 IGNTPERLFHREGLGIKSDALAATRARGRTAIEDIKIEHDLFSNPKEDLEFSIVRETIKK 420
Query: 428 KLEA-----IVKPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCGLPTEE 487
KLEA +V+P+KSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCGLPTEE
Sbjct: 421 KLEAVCDAVVVEPYKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCGLPTEE 480
Query: 488 ARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVQRGLGALIYAGTGIVKGSNPS 547
ARD IAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALV+RGLGALIYAGTGIVKGSNPS
Sbjct: 481 ARDFIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVKRGLGALIYAGTGIVKGSNPS 540
Query: 548 SEWDELDLKISQVTRSLKLEVAQPP 552
SEWDEL+LKISQ++RSL+LEV P
Sbjct: 541 SEWDELELKISQISRSLELEVVHAP 562
BLAST of Spg022055 vs. ExPASy TrEMBL
Match:
A0A5D3BJQ7 (Isochorismate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G002380 PE=3 SV=1)
HSP 1 Score: 861.3 bits (2224), Expect = 2.3e-246
Identity = 445/570 (78.07%), Postives = 479/570 (84.04%), Query Frame = 0
Query: 3 ISKMAVSSTQNSAT-GECVWFRQHVSWRRRIPAVVAWPRRWRRWGGVVCQWPAMDGCRG- 62
++ +AVSS QNSAT + F Q S RRI A VAWP R RRWG VC AMDGC G
Sbjct: 4 LNNVAVSSNQNSATCRHFIGFHQRGSRCRRISAAVAWPERGRRWGEEVCDSAAMDGCGGG 63
Query: 63 ---DERGAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGIIRLQVPIQQHVQ 122
+ G VGTIETR+FPAV TAALA ESLR VA+LKSEAP +SSGIIRLQVPIQQ +Q
Sbjct: 64 NNQEAGGTVGTIETRNFPAVRTAALATESLRRGVAELKSEAPALSSGIIRLQVPIQQRIQ 123
Query: 123 AIDWLCSQPHLLPRTFFSGRSRAIDPHPLSADNTADVRHPFNDHHLVGVAGVGSAVVFCR 182
AIDWL SQPHLLPRTFFSGRSR D SA N ++ H FN HL+GVAGVGSAVVF R
Sbjct: 124 AIDWLSSQPHLLPRTFFSGRSRPTDS---SAHNISNGSHSFNHRHLLGVAGVGSAVVFRR 183
Query: 183 LHRFSNHDWASIKRFLSKKCPLIRAYGGIRFDSRANISSEWEPFGSFYFMVPQVEFDEFE 242
LH FS +DWASIKRFLSKKCPLIRAYGGIRFDSRANIS EWEPFGSFYFMVPQVEFDEFE
Sbjct: 184 LHCFSYNDWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFE 243
Query: 243 DNSMLAATVVWDHACSWTWENAVESLQSTIEQVSSNNVNLQRDLPHISIFSHNHVPSKTF 302
+NSMLAATV WDHACSWTWENAVESLQSTIEQVSSNNVNLQ+D P I I S+ HVP KTF
Sbjct: 244 ENSMLAATVAWDHACSWTWENAVESLQSTIEQVSSNNVNLQKDPPQIPILSYTHVPGKTF 303
Query: 303 WDRAIDRALQ-----------VVLARSTRIVTSVDVDPVAWVACLQKEGKNSYQFCVQPY 362
W+RA+DRALQ VVLARST+IVT VD+DPV +AC QKEG NSYQFCVQP
Sbjct: 304 WERAVDRALQEIDGSNSELTKVVLARSTQIVTGVDIDPVTCIACFQKEGNNSYQFCVQPS 363
Query: 363 NAPAFIGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIEHDLFSNPKDDLEFSIVR 422
NA AFIG+TPERLFHRE L IKS+ALAATRARGRSAIED+KIEHDLFSNPK+DLEFSIVR
Sbjct: 364 NAHAFIGNTPERLFHREGLDIKSDALAATRARGRSAIEDIKIEHDLFSNPKEDLEFSIVR 423
Query: 423 ETIKRKLEA-----IVKPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCG 482
E IK+KLEA +V+P+KSVRKLPRIQHLYAQLTGRLRSEEDEFEILS LHPTPAVCG
Sbjct: 424 EAIKKKLEAVCDAVVVEPYKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSMLHPTPAVCG 483
Query: 483 LPTEEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVQRGLGALIYAGTGIVK 542
PTEEARD IAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALV+RGLGALIYAGTGIVK
Sbjct: 484 WPTEEARDFIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVKRGLGALIYAGTGIVK 543
Query: 543 GSNPSSEWDELDLKISQVTRSLKLEVAQPP 552
GSNPSSEWDEL+LKISQ++RSL+LEV Q P
Sbjct: 544 GSNPSSEWDELELKISQISRSLELEVVQEP 570
BLAST of Spg022055 vs. ExPASy TrEMBL
Match:
A0A1S3BV15 (Isochorismate synthase OS=Cucumis melo OX=3656 GN=LOC103493974 PE=3 SV=1)
HSP 1 Score: 861.3 bits (2224), Expect = 2.3e-246
Identity = 445/570 (78.07%), Postives = 479/570 (84.04%), Query Frame = 0
Query: 3 ISKMAVSSTQNSAT-GECVWFRQHVSWRRRIPAVVAWPRRWRRWGGVVCQWPAMDGCRG- 62
++ +AVSS QNSAT + F Q S RRI A VAWP R RRWG VC AMDGC G
Sbjct: 4 LNNVAVSSNQNSATCRHFIGFHQRGSRCRRISAAVAWPERGRRWGEKVCDSAAMDGCGGG 63
Query: 63 ---DERGAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGIIRLQVPIQQHVQ 122
+ G VGTIETR+FPAV TAALA ESLR VA+LKSEAP +SSGIIRLQVPIQQ +Q
Sbjct: 64 NNQEAGGTVGTIETRNFPAVRTAALATESLRRGVAELKSEAPALSSGIIRLQVPIQQRIQ 123
Query: 123 AIDWLCSQPHLLPRTFFSGRSRAIDPHPLSADNTADVRHPFNDHHLVGVAGVGSAVVFCR 182
AIDWL SQPHLLPRTFFSGRSR D SA N ++ H FN HL+GVAGVGSAVVF R
Sbjct: 124 AIDWLSSQPHLLPRTFFSGRSRPTDS---SAHNISNGSHSFNHRHLLGVAGVGSAVVFRR 183
Query: 183 LHRFSNHDWASIKRFLSKKCPLIRAYGGIRFDSRANISSEWEPFGSFYFMVPQVEFDEFE 242
LH FS +DWASIKRFLSKKCPLIRAYGGIRFDSRANIS EWEPFGSFYFMVPQVEFDEFE
Sbjct: 184 LHCFSYNDWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFE 243
Query: 243 DNSMLAATVVWDHACSWTWENAVESLQSTIEQVSSNNVNLQRDLPHISIFSHNHVPSKTF 302
+NSMLAATV WDHACSWTWENAVESLQSTIEQVSSNNVNLQ+D P I I S+ HVP KTF
Sbjct: 244 ENSMLAATVAWDHACSWTWENAVESLQSTIEQVSSNNVNLQKDPPQIPILSYTHVPGKTF 303
Query: 303 WDRAIDRALQ-----------VVLARSTRIVTSVDVDPVAWVACLQKEGKNSYQFCVQPY 362
W+RA+DRALQ VVLARST+IVT VD+DPV +AC QKEG NSYQFCVQP
Sbjct: 304 WERAVDRALQEIDGSNSELTKVVLARSTQIVTGVDIDPVTCIACFQKEGNNSYQFCVQPS 363
Query: 363 NAPAFIGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIEHDLFSNPKDDLEFSIVR 422
NA AFIG+TPERLFHRE L IKS+ALAATRARGRSAIED+KIEHDLFSNPK+DLEFSIVR
Sbjct: 364 NAHAFIGNTPERLFHREGLDIKSDALAATRARGRSAIEDIKIEHDLFSNPKEDLEFSIVR 423
Query: 423 ETIKRKLEA-----IVKPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCG 482
E IK+KLEA +V+P+KSVRKLPRIQHLYAQLTGRLRSEEDEFEILS LHPTPAVCG
Sbjct: 424 EAIKKKLEAVCDAVVVEPYKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSMLHPTPAVCG 483
Query: 483 LPTEEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVQRGLGALIYAGTGIVK 542
PTEEARD IAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALV+RGLGALIYAGTGIVK
Sbjct: 484 WPTEEARDFIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVKRGLGALIYAGTGIVK 543
Query: 543 GSNPSSEWDELDLKISQVTRSLKLEVAQPP 552
GSNPSSEWDEL+LKISQ++RSL+LEV Q P
Sbjct: 544 GSNPSSEWDELELKISQISRSLELEVVQEP 570
BLAST of Spg022055 vs. TAIR 10
Match:
AT1G18870.1 (isochorismate synthase 2 )
HSP 1 Score: 597.0 bits (1538), Expect = 1.6e-170
Identity = 310/517 (59.96%), Postives = 386/517 (74.66%), Query Frame = 0
Query: 54 AMDGCRGDERGAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGIIRLQVPIQ 113
+M+GC D + +GT+ETR+ V + A A E L V+ LKS+ PP SSGI+RLQVPI+
Sbjct: 50 SMNGCEADHKAPLGTVETRTLSTVPSPAAATERLITAVSDLKSQPPPFSSGIVRLQVPIE 109
Query: 114 QHVQAIDWLCSQPHLLPRTFFSGRSRAIDPHPL---SADNTADVRHPFNDHHLVGVAGVG 173
Q + AIDWL +Q +LPR+FFS RS + P L S+DN + +DH+ V VAG+G
Sbjct: 110 QKIGAIDWLHAQNEILPRSFFSRRSDSGRPDLLQDFSSDNGS------SDHNPVSVAGIG 169
Query: 174 SAVVFCRLHRFSNHDWASIKRFLSKKCPLIRAYGGIRFDSRANISSEWEPFGSFYFMVPQ 233
SAV F L FS+ DW SI+RFLS K PLIRAYGG+RFD I+ EWE FGSFYF VPQ
Sbjct: 170 SAVFFRDLDPFSHDDWRSIRRFLSSKSPLIRAYGGLRFDPTGKIAVEWEHFGSFYFTVPQ 229
Query: 234 VEFDEFEDNSMLAATVVWDHACSWTWENAVESLQSTIEQVSSNNVNLQRDLPHISIFSHN 293
VEFDEF +SMLAATV WD+ SWT ENA+E+LQ T+ QVSS + L+R+ + + S N
Sbjct: 230 VEFDEFGGSSMLAATVAWDNELSWTLENAIEALQETMLQVSSVIMRLRRESLGVIVVSKN 289
Query: 294 HVPSKTFWDRAIDRALQ-----------VVLARSTRIVTSVDVDPVAWVACLQKEGKNSY 353
HVPS+ + A++ AL+ VVLARS+RI+T D+DP+AW+A LQ EG+++Y
Sbjct: 290 HVPSEGAYYPAVNNALEIIKDKHSPLSKVVLARSSRIITDTDIDPIAWLARLQCEGQDAY 349
Query: 354 QFCVQPYNAPAFIGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIEHDLFSNPKDD 413
QFC+QP APAFIG+TPERLFHR+ L + SEALAATR RG S + +++IE DL ++PKDD
Sbjct: 350 QFCLQPPGAPAFIGNTPERLFHRKHLGVCSEALAATRPRGDSKVREMEIERDLLTSPKDD 409
Query: 414 LEFSIVRETIKRKLEAI-----VKPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLH 473
LEFSIVRE I+ KL+ I VKPHKSVRKL R+QHLY+QL G+L+ E+DEF IL+ LH
Sbjct: 410 LEFSIVRENIREKLKTICDRVVVKPHKSVRKLARVQHLYSQLAGQLKREDDEFNILTALH 469
Query: 474 PTPAVCGLPTEEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVQRGLGALIY 533
PTPAVCG P EEAR LI + E FDRGMYAGP+G+FGGGESEF+VGIRSALV++GLGALIY
Sbjct: 470 PTPAVCGCPVEEARLLIKQIESFDRGMYAGPIGFFGGGESEFSVGIRSALVEKGLGALIY 529
Query: 534 AGTGIVKGSNPSSEWDELDLKISQVTRSLKLEVAQPP 552
AGTGIV GSNPSSEW+EL+LKISQ T+SL+ E A P
Sbjct: 530 AGTGIVSGSNPSSEWNELELKISQFTKSLEHESALQP 560
BLAST of Spg022055 vs. TAIR 10
Match:
AT1G18870.2 (isochorismate synthase 2 )
HSP 1 Score: 596.7 bits (1537), Expect = 2.0e-170
Identity = 310/516 (60.08%), Postives = 385/516 (74.61%), Query Frame = 0
Query: 55 MDGCRGDERGAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGIIRLQVPIQQ 114
M+GC D + +GT+ETR+ V + A A E L V+ LKS+ PP SSGI+RLQVPI+Q
Sbjct: 1 MNGCEADHKAPLGTVETRTLSTVPSPAAATERLITAVSDLKSQPPPFSSGIVRLQVPIEQ 60
Query: 115 HVQAIDWLCSQPHLLPRTFFSGRSRAIDPHPL---SADNTADVRHPFNDHHLVGVAGVGS 174
+ AIDWL +Q +LPR+FFS RS + P L S+DN + +DH+ V VAG+GS
Sbjct: 61 KIGAIDWLHAQNEILPRSFFSRRSDSGRPDLLQDFSSDNGS------SDHNPVSVAGIGS 120
Query: 175 AVVFCRLHRFSNHDWASIKRFLSKKCPLIRAYGGIRFDSRANISSEWEPFGSFYFMVPQV 234
AV F L FS+ DW SI+RFLS K PLIRAYGG+RFD I+ EWE FGSFYF VPQV
Sbjct: 121 AVFFRDLDPFSHDDWRSIRRFLSSKSPLIRAYGGLRFDPTGKIAVEWEHFGSFYFTVPQV 180
Query: 235 EFDEFEDNSMLAATVVWDHACSWTWENAVESLQSTIEQVSSNNVNLQRDLPHISIFSHNH 294
EFDEF +SMLAATV WD+ SWT ENA+E+LQ T+ QVSS + L+R+ + + S NH
Sbjct: 181 EFDEFGGSSMLAATVAWDNELSWTLENAIEALQETMLQVSSVIMRLRRESLGVIVVSKNH 240
Query: 295 VPSKTFWDRAIDRALQ-----------VVLARSTRIVTSVDVDPVAWVACLQKEGKNSYQ 354
VPS+ + A++ AL+ VVLARS+RI+T D+DP+AW+A LQ EG+++YQ
Sbjct: 241 VPSEGAYYPAVNNALEIIKDKHSPLSKVVLARSSRIITDTDIDPIAWLARLQCEGQDAYQ 300
Query: 355 FCVQPYNAPAFIGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIEHDLFSNPKDDL 414
FC+QP APAFIG+TPERLFHR+ L + SEALAATR RG S + +++IE DL ++PKDDL
Sbjct: 301 FCLQPPGAPAFIGNTPERLFHRKHLGVCSEALAATRPRGDSKVREMEIERDLLTSPKDDL 360
Query: 415 EFSIVRETIKRKLEAI-----VKPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHP 474
EFSIVRE I+ KL+ I VKPHKSVRKL R+QHLY+QL G+L+ E+DEF IL+ LHP
Sbjct: 361 EFSIVRENIREKLKTICDRVVVKPHKSVRKLARVQHLYSQLAGQLKREDDEFNILTALHP 420
Query: 475 TPAVCGLPTEEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVQRGLGALIYA 534
TPAVCG P EEAR LI + E FDRGMYAGP+G+FGGGESEF+VGIRSALV++GLGALIYA
Sbjct: 421 TPAVCGCPVEEARLLIKQIESFDRGMYAGPIGFFGGGESEFSVGIRSALVEKGLGALIYA 480
Query: 535 GTGIVKGSNPSSEWDELDLKISQVTRSLKLEVAQPP 552
GTGIV GSNPSSEW+EL+LKISQ T+SL+ E A P
Sbjct: 481 GTGIVSGSNPSSEWNELELKISQFTKSLEHESALQP 510
BLAST of Spg022055 vs. TAIR 10
Match:
AT1G74710.1 (ADC synthase superfamily protein )
HSP 1 Score: 572.0 bits (1473), Expect = 5.4e-163
Identity = 299/514 (58.17%), Postives = 372/514 (72.37%), Query Frame = 0
Query: 54 AMDGCRGDERGAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGIIRLQVPIQ 113
+M+GC GD + +GT+ETR+ AV + A A E L V++LKS+ P SSG++RLQVPI
Sbjct: 47 SMNGCDGDFKTPLGTVETRTMTAVLSPAAATERLISAVSELKSQPPSFSSGVVRLQVPID 106
Query: 114 QHVQAIDWLCSQPHLLPRTFFSGRSRAIDPHPL-----SADNTADVRHPFNDHHLVGVAG 173
Q + AIDWL +Q + PR FFS RS P L N +D +LV VAG
Sbjct: 107 QQIGAIDWLQAQNEIQPRCFFSRRSDVGRPDLLLDLANENGNGNGNGTVSSDRNLVSVAG 166
Query: 174 VGSAVVFCRLHRFSNHDWASIKRFLSKKCPLIRAYGGIRFDSRANISSEWEPFGSFYFMV 233
+GSAV F L FS+ DW SI+RFLS PLIRAYGG+RFD I+ EWEPFG+FYF V
Sbjct: 167 IGSAVFFRDLDPFSHDDWRSIRRFLSSTSPLIRAYGGMRFDPNGKIAVEWEPFGAFYFSV 226
Query: 234 PQVEFDEFEDNSMLAATVVWDHACSWTWENAVESLQSTIEQVSSNNVNLQRDLPHISIFS 293
PQVEF+EF +SMLAAT+ WD SWT ENA+E+LQ T+ QVSS + L+ +S+ S
Sbjct: 227 PQVEFNEFGGSSMLAATIAWDDELSWTLENAIEALQETMLQVSSVVMKLRNRSLGVSVLS 286
Query: 294 HNHVPSKTFWDRAIDRALQ-----------VVLARSTRIVTSVDVDPVAWVACLQKEGKN 353
NHVP+K + A+++AL+ VVLAR++RI+T D+DP+AW+A LQ+EG +
Sbjct: 287 KNHVPTKGAYFPAVEKALEMINQKSSPLNKVVLARNSRIITDTDIDPIAWLAQLQREGHD 346
Query: 354 SYQFCVQPYNAPAFIGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIEHDLFSNPK 413
+YQFC+QP APAFIG+TPERLF R L + SEALAATR R S+ D++IE DL ++PK
Sbjct: 347 AYQFCLQPPGAPAFIGNTPERLFQRTQLGVCSEALAATRPRAASSARDMEIERDLLTSPK 406
Query: 414 DDLEFSIVRETIKRKLEAI-----VKPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILST 473
DDLEFSIVRE I+ KL I VKP K+VRKL R+QHLY+QL GRL E+DE++IL+
Sbjct: 407 DDLEFSIVRENIREKLNGICDRVVVKPQKTVRKLARVQHLYSQLAGRLTKEDDEYKILAA 466
Query: 474 LHPTPAVCGLPTEEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVQRGLGAL 533
LHPTPAVCGLP EEAR LI E E FDRGMYAGP+G+FGG ESEFAVGIRSALV++GLGAL
Sbjct: 467 LHPTPAVCGLPAEEARLLIKEIESFDRGMYAGPIGFFGGEESEFAVGIRSALVEKGLGAL 526
Query: 534 IYAGTGIVKGSNPSSEWDELDLKISQVTRSLKLE 547
IYAGTGIV GS+PSSEW+ELDLKISQ T+S++ E
Sbjct: 527 IYAGTGIVAGSDPSSEWNELDLKISQFTKSIEYE 560
BLAST of Spg022055 vs. TAIR 10
Match:
AT1G74710.2 (ADC synthase superfamily protein )
HSP 1 Score: 567.0 bits (1460), Expect = 1.7e-161
Identity = 297/507 (58.58%), Postives = 367/507 (72.39%), Query Frame = 0
Query: 54 AMDGCRGDERGAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGIIRLQVPIQ 113
+M+GC GD + +GT+ETR+ AV + A A E L V++LKS+ P SSG++RLQVPI
Sbjct: 47 SMNGCDGDFKTPLGTVETRTMTAVLSPAAATERLISAVSELKSQPPSFSSGVVRLQVPID 106
Query: 114 QHVQAIDWLCSQPHLLPRTFFSGRSRAIDPHPL-----SADNTADVRHPFNDHHLVGVAG 173
Q + AIDWL +Q + PR FFS RS P L N +D +LV VAG
Sbjct: 107 QQIGAIDWLQAQNEIQPRCFFSRRSDVGRPDLLLDLANENGNGNGNGTVSSDRNLVSVAG 166
Query: 174 VGSAVVFCRLHRFSNHDWASIKRFLSKKCPLIRAYGGIRFDSRANISSEWEPFGSFYFMV 233
+GSAV F L FS+ DW SI+RFLS PLIRAYGG+RFD I+ EWEPFG+FYF V
Sbjct: 167 IGSAVFFRDLDPFSHDDWRSIRRFLSSTSPLIRAYGGMRFDPNGKIAVEWEPFGAFYFSV 226
Query: 234 PQVEFDEFEDNSMLAATVVWDHACSWTWENAVESLQSTIEQVSSNNVNLQRDLPHISIFS 293
PQVEF+EF +SMLAAT+ WD SWT ENA+E+LQ T+ QVSS + L+ +S+ S
Sbjct: 227 PQVEFNEFGGSSMLAATIAWDDELSWTLENAIEALQETMLQVSSVVMKLRNRSLGVSVLS 286
Query: 294 HNHVPSKTFWDRAIDRALQ-----------VVLARSTRIVTSVDVDPVAWVACLQKEGKN 353
NHVP+K + A+++AL+ VVLAR++RI+T D+DP+AW+A LQ+EG +
Sbjct: 287 KNHVPTKGAYFPAVEKALEMINQKSSPLNKVVLARNSRIITDTDIDPIAWLAQLQREGHD 346
Query: 354 SYQFCVQPYNAPAFIGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIEHDLFSNPK 413
+YQFC+QP APAFIG+TPERLF R L + SEALAATR R S+ D++IE DL ++PK
Sbjct: 347 AYQFCLQPPGAPAFIGNTPERLFQRTQLGVCSEALAATRPRAASSARDMEIERDLLTSPK 406
Query: 414 DDLEFSIVRETIKRKLEAI-----VKPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILST 473
DDLEFSIVRE I+ KL I VKP K+VRKL R+QHLY+QL GRL E+DE++IL+
Sbjct: 407 DDLEFSIVRENIREKLNGICDRVVVKPQKTVRKLARVQHLYSQLAGRLTKEDDEYKILAA 466
Query: 474 LHPTPAVCGLPTEEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVQRGLGAL 533
LHPTPAVCGLP EEAR LI E E FDRGMYAGP+G+FGG ESEFAVGIRSALV++GLGAL
Sbjct: 467 LHPTPAVCGLPAEEARLLIKEIESFDRGMYAGPIGFFGGEESEFAVGIRSALVEKGLGAL 526
Query: 534 IYAGTGIVKGSNPSSEWDELDLKISQV 540
IYAGTGIV GS+PSSEW+ELDLKISQV
Sbjct: 527 IYAGTGIVAGSDPSSEWNELDLKISQV 553
BLAST of Spg022055 vs. TAIR 10
Match:
AT1G68890.1 (magnesium ion binding;thiamin pyrophosphate binding;hydro-lyases;catalytics;2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthases )
HSP 1 Score: 114.0 bits (284), Expect = 4.0e-25
Identity = 78/233 (33.48%), Postives = 119/233 (51.07%), Query Frame = 0
Query: 47 GVVCQWPAMD-GCRGDERGAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGI 106
GV P MD D+ V TR+ P T L ESL+ + +LK+ P SSG+
Sbjct: 56 GVQFDGPIMDRDVNLDDDLVVQVCVTRTLPPALTLELGLESLKEAIDELKTNPPKSSSGV 115
Query: 107 IRLQVPIQQHVQAIDWLCSQP---HLLPRTFFSGRSRAIDPHPLSADNTADVRHPFNDHH 166
+R QV + +A+ W CSQP + P F S ++P + + V+ P
Sbjct: 116 LRFQVAVPPRAKALFWFCSQPTTSDVFPVFFLS--KDTVEP----SYKSLYVKEPH---- 175
Query: 167 LVGVAGVGSAVVFCRLHRFSNHDWASIKRFLSKKCPLIRAYG--GIRFDSRANISSEWEP 226
GV G+G+A F ++ + IK FLS + ++ AYG I F+ + ++S+
Sbjct: 176 --GVFGIGNAFAFVHSSSVDSNGHSMIKTFLSDESAMVTAYGFPDIEFNKYSTVNSK--- 235
Query: 227 FGSFYFMVPQVEFDEFEDNSMLAATVVWDHACSWTWENAVESLQSTIEQVSSN 274
GS YF VPQ+E DE E+ S+LA T+ W+ + S+T E + S + +I QVSS+
Sbjct: 236 DGSSYFFVPQIELDEHEEVSILAVTLAWNESLSYTVEQTISSYEKSIFQVSSH 273
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_038879640.1 | 2.8e-262 | 82.81 | isochorismate synthase 2, chloroplastic isoform X1 [Benincasa hispida] | [more] |
XP_038879641.1 | 2.8e-254 | 81.23 | isochorismate synthase 2, chloroplastic isoform X2 [Benincasa hispida] | [more] |
XP_022932292.1 | 3.2e-250 | 79.68 | isochorismate synthase 2, chloroplastic-like isoform X1 [Cucurbita moschata] | [more] |
XP_022972451.1 | 5.5e-250 | 79.37 | isochorismate synthase 2, chloroplastic isoform X1 [Cucurbita maxima] | [more] |
XP_023530897.1 | 1.5e-247 | 78.84 | isochorismate synthase 2, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo] | [more] |
Match Name | E-value | Identity | Description | |
Q9M9V6 | 2.2e-169 | 59.96 | Isochorismate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ICS2 ... | [more] |
Q9ZPC0 | 1.2e-162 | 56.32 | Isochorismate synthase, chloroplastic OS=Catharanthus roseus OX=4058 PE=1 SV=1 | [more] |
Q9S7H8 | 7.6e-162 | 58.17 | Isochorismate synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ICS1 ... | [more] |
P23973 | 5.1e-33 | 29.67 | Isochorismate synthase MenF OS=Bacillus subtilis (strain 168) OX=224308 GN=menF ... | [more] |
P38051 | 2.8e-31 | 34.05 | Isochorismate synthase MenF OS=Escherichia coli (strain K12) OX=83333 GN=menF PE... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1EW89 | 1.6e-250 | 79.68 | Isochorismate synthase OS=Cucurbita moschata OX=3662 GN=LOC111438650 PE=3 SV=1 | [more] |
A0A6J1I605 | 2.6e-250 | 79.37 | Isochorismate synthase OS=Cucurbita maxima OX=3661 GN=LOC111471007 PE=3 SV=1 | [more] |
A0A0A0LS91 | 8.0e-247 | 78.58 | Isochorismate synthase OS=Cucumis sativus OX=3659 GN=Csa_1G008580 PE=3 SV=1 | [more] |
A0A5D3BJQ7 | 2.3e-246 | 78.07 | Isochorismate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3... | [more] |
A0A1S3BV15 | 2.3e-246 | 78.07 | Isochorismate synthase OS=Cucumis melo OX=3656 GN=LOC103493974 PE=3 SV=1 | [more] |