Spg022055 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg022055
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionIsochorismate synthase
Locationscaffold2: 5060460 .. 5069556 (+)
RNA-Seq ExpressionSpg022055
SyntenySpg022055
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TCACCAACCTCTAGAAAACCGACAACAACTAAAACCGACCGACCGATTTGTCGGTGGTCGGTGTCGGTTTTGGCCCAAAACCGACTCCGACCGACCGATGAACACCCCTAATTGAAAGGAGCAGTGCAATGCTTCAATTTCATATAATTATTTGAAAGTTGGTTATTTGGGGATGGCGAGAGTTGGGATTTGGTTTAGAGAAAGAAAGAAATAAAAATAAGTTTGCTTTCCAAAAATGAGCATTTCCAAAATGGCTGTTTCTTCGACTCAAAATTCAGCCACCGGAGAGTGTGTTTGGTTCCGCCAGCATGTGAGTTGGCGGCGCCGCATTCCGGCAGTCGTAGCTTGGCCGAGGCGGTGGCGGAGGTGGGGCGGGGTGGTTTGCCAGTGGCCGGCGATGGATGGGTGCAGAGGAGACGAGAGAGGGGCCGTCGGGACGATTGAGACTCGCAGTTTTCCGGCGGTTGAGACGGCGGCTTTGGCGGCGGAGAGTCTGAGGGGAGAAGTGGCAAAGTTGAAGTCAGAGGCACCGCCAGTGAGTTCTGGAATTATTCGGCTACAAGTAAGCCTCCTTTGAACTTGAGACCTCTTTCAAATGGGTCGATTCCATTAGTTTCTTATCTCTTCTCTGTACGTGTGGAATGGCGCGGCGTACTGCCACGTGCTCCTAACATTTAAATGTAGGGGTGTTCAAAAAAGCTCTCAAAACCGACTCGACCGACCGAAACCGACCGAACCGATGTCGGTCGGGGTCGGTCGGTCGGAGGAAAAATTTGAAAATTTCCGACCGACCGACCGAATGTAGACCACATAAATATGTCTCTTCCGTTTTCCCACGTAAATCATTAATGAAAAACCAAGAAAGTAAAAAAAAATGATCACTTTTTCGTTTTCCAAGGAAGGCATTAATGAAAAAACCATGGTTGCCCATGGTTTTCTCAAAACCACTTATGGTTTTACCAAAATAATGATTACTTTTTTTATCTCTCTCCTCTCTCTTCACTCTTCCACGATTCCTTCCCATTTTCATTTCAAAATTCGACTTTCTTCTCTCTCTTTCTTTCTCTTTCCCATCTTCCCTCCCAACTCTTCTTCCTCATTCTTTCTCTTCTCTTCCCTCCTCCCACCGGCGAAGCCATCACCGCCGTCCGTTTCTCCTCACTTCTCTTATTTTTTTTTTCTTTTCTTTCCTCTTTTGGGTTAGTTCACGGAGATAAAAACCGACCATGAATATTTCTTTCTTCTTTTTTCTCCTTATTTCTCTCTTCTCTTTTTTTTCTTTCTATTTTCTCCCTTTTCACTGTGATTTGGTTCGCCGGAGTAAACCGACCGAAACCGACTGACCGACTGTGAGTTCGGTCGGTCGGCGTCACCAACCTCTAGAAAACCGACAACAACTAAAACCGACCGACCGATTTGTCGGTGGTCGACGTCGGTTTTGGCCCAAAACCGACTCCGACCGACTGATGAACACCCCTATTTAAATGAGAGATTTTTCTTGGTTTGAAATATATTTTAATTTAAATGTTTTTCAAAAATGCATTTTTTTAAAAACATTTTGTATAAAAATGGTTTAGAATATAAACACTCATATGTTTGGTTTGGTTGCATTTCCTCTGTTTTTATACACAAATTATTATATAGTTAAATTTTTTTTTTAAAAAAAGTTGGTTTTAGGTGTTTCTCTCAAAATGGTTTATTTATGAGTCTCATTTTCTAATAAATCATTTTTAGTTGTTGCCAAACACTAAATTTTTTTTCTCAAAATTAAACATTTGAAAAGTTAAACTAAACACACCCTAAGAATAAATTCGGATTTTGTTTACTTTTTTTTTCAGATGTCAATTGTTTTGGAAAGTTTTTTTTTAGAAGGCCATCAAGAGTCTGTTTGGAATACTTTTCAGTTTAAGTATTTTTCAAGAATAGTATAAAAATTATTTAGAATTTAAACACTCGTATGTTTAGTTACATTTCCTTATAAGTGTTTTTATACACAAGTTATTATATACTTAAAAAAATTTGTTTTGAAGTATTTCTCTCAAAATGGTTTATTTCTCAATCTCTTTTTTAATAAGTCGTTTGTAGTGTTTGTCAAACACTAGATTTTTTTTTAAAAAATAAACACTTAAAAAGTAAATCAAATACACCCTAAGTTAAGTGGATTGTAGGTTGATTTATGAAAAAAACGATTAACATTCTTGAGGTAGCAATTTTTGAATGAGGTATTTATATTATAATTAATTTTAGTACAGTACTTGACTGCATATTGATCTGTAGGATCTCTCCATATATTCTATTAAAATCTTGACAACTAAGAGTTCACAAGGTAGAAAATGAGAAATTTATTTTATCTTTCTTAATTGACGACGTTTTAATGCTTGTATGTAGGAAGATCCTTACACATCGGTATACTGCCAATTATTTTCTTTAATTTTATAACTTTTTAGTTTATGTGGCCTTTTATTTTCTTCTTTCCTTTTGTCGAGCATCATTGATTAGAGACTATTGTTACATTATTATTGGTGGATCACACCTCATTCTCTCTTCTCTTCTCATTGGCCTTCTTCTTCTTCTCCTCTTTCTTCAATTGTTTCCCTTTTCTTCATTTCATTCTCATATTTTCCCCCTCCTAGCTTCTAGATCTGAACATTTTGTTATAAGTCTAACTATTTCAAAATGTCTAGATTTGGAACAAATATACTTCAAGAAGTAAGGGAGTGAGAGAAAATGATAGATAGTAGGAATGGCTATCAGAAGAAGAGAAATAGAAGGAAATGACCAGAGGAAGAGGATGGAAGAAGGAAGAGAAATGAAGAGAGAGGAAGAAAGAGAACCATGGCAATCTACTTAGAAGTAGAACATACACAAGTACTTATACTAGAGGAAAAGTTATTCAATAAGAGTGCGATGATGTGGTTGTCTCTTAACAACGGTTGCATGTGAATTCAAAATCATGTATAACTCTTAATAACTTTTAGATACAGATTATATTTGAAATATTTTTACAGTTTCTCTCTCATATGTTCAATAGTGTAAGACGGTTTGTCGGTTATCATCTCCTCCAATAATCTCTCCTACTAAAGGAGGTAGAGAAGGAGAAATAGGTACAACAGCTAAAAACAATGTAAAATTAAGGCTAAATTATAAAAAATACCTCTGAATTATGAGATTTGTTTCAAAAATGCTCCTGAACTTTGAAAAGTTTCAAAACTACCCCTGAACTTTGAAAAATGTTTCAAAAATACCCTTGCCCTTAATTTTTGGATGGAAACCGTTAGAATTTACGGTGAGATGAGGAAAAAAGAAAAATCATTTTTCAATAAGGGTATTTTTTAAAATTTTTTTGAAAGTTCAAGGGTATTTTTCAAACTGAAACTTTAACGGTTTCCATCCAAAAGTTAAGGGCCATGGTATTTTTGAAGCATTTTTCAAAGTTCAGGAGTATTTTTGAAACTTTTCAAAGTTCAGGGGCATTTTTGAAACAAACCCCATAGTTCAGGGGTATTTTTTATAATTTAGCCTAAAATTAATATAAATGGACATCCCAAAGTTGTCATCTAGCTAGACAAAGCCACTAATGGTCACAAGGTAAATTGTCATCAAGACAAAGTTACACTAATGGTCACAACATATAATTAATATACCATTAATGTAATAAGTTGTCATCTCATTTAAATAATGGAGAACTCCATTATTTTTCCATCAGACAGTCAACTAATGGTGAACTATTTAGAACTGTTTTTTTTTTTTTTGTTTTTTAAATATAATGAACTGAATGGTTGCTTTTGAAACCATGGTGACCAAGTGTAAACCATTCCAAAACCTCGAGAACTAATTGTGTAATTTATCCCAAGCTTAGTCAATTTTGTCCATTCAAGCATAATTCGATGGATAAGACACAAATTATCATTGTAAAGGTCGATGGTTTAATTCTTCATTACTGTAATTATTGAACTAAAAAAAATAGTTAAGACAACTTTTTATTGTTGTTAAAAAAGGCTAATGGTATGTTTTAGTCCCTAAGGAGTTTTACAATCTAATCTCTATACTTTTAAAATGTTCAATTTAGTCCCTTTAGTTTTAACATTTTTTTAATGAAAAATCAATTATATCACTTTTCTTTTGTGAATGAATAAACTTACCTGATAAGATACTTACTCAATTAGTAGGAATTTAGAGGAAGAATTATGTAACTTGAGAGAGAAAAAAATGGTCGAAACTTCTATAAAATCTAGAATAGGAACCAGAAACTTTCATCACTTATGTAGCCTAATTCCTTCCTACGATTCTATCTATCCATTAAGTATCATGTCATATAAATTTATTATCGACAATTTTTCATTGACCAATCAAAGAGTATAATATATTGAAAATTAAAGTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATAAGTGATTAAATTAAACCTTTTTGAACCATGGGGACTAAATTAAAAAAAAATAAACACTCAAACAGAGAGTAAATATTCAAACCTCTAAACAGTTTAAGTCAAATGTATAATACTTTAAGTTCTTGCCCATGTATGGATTGTATAGCCTTTTTGTTGCCAATATCTTGAAATATGATTTTTATAATATGAACTAAACATAATTTTAAGAAAGTTGAAAAATCATAAAATAAATTACTCCCTGGAATACCTGAAATTGTGCTTCTTTTCTCAATTTATTTTCAATGAGTTTTATCTTTCTTAGTTAAATACTTGACTTCATAACCAAATTTAAAATACAAAAACAAATTTTTAAAAACTACATTTTTTTTTTTAATTTTCAAAACATAGTTAAGATTTTAAAAAAATCGTAAAAAGTCGATAACAAAAACAAATAAACTCATAGATAAAAATAGTGTTTACAGTCTAAATTTTAAAAAATAAAAAATAAAAAACTAAATAGTTATTAAATTCTGGTAGAGTTTTACTAACTTTGTGTAAAAATATAATAAAGGATTCAAAATTGTATCTAAGCATCACCTATTGTTTCTATTATCGATTCATATTTATAATTTATTTATTTCTTTGACTGATTATGATTGAATAATTAAAAAAATAAAAAAAATAGTTAAAAAAAATAATAATAAATAAAGTTTAGCGGTTCTAACTTTGGAATTTGAAATTGTTCATGCCTGAATACAGTGGCAGAGTGTTTTTCCCGCGGAATATAACTACTTTCTGAGACTGAAGGAGGCTGTACTAATTTGCAGGTCCCAATACAACAGCACGTTCAAGCAATTGATTGGCTCTGTTCTCAGCCCCACCTTCTTCCTCGCACCTTCTTCTCCGGCAGAAGCAGAGCCATTGATCCTCATCCTCTCTCCGCCGACAACACTGCCGATGTCCGCCATCCCTTCAACGATCACCACCTGGTCGGCGTCGCCGGTGTGGGCTCTGCCGTCGTCTTCTGTCGCCTTCATCGATTTTCGAACCACGATTGGGCGTCTATAAAGAGGTAAACTAATTTTGCCTCCAGTTTCCATCCATTCTGCCATCTGTTCGATGAAATGTCTACAATGGGTAATATTCTATATATTCTAAAATTGAAGGTTCCTCTCAAAAAAATGCCCCCTGATTCGTGCTTATGGAGGCATTCGCTTTGATTCACGGGCCAACATATCATCGGAGTGGGAGCCTTTTGGCTCGTTTTACTTCATGGTCCCTCAGGTAGAGTAGAGACGTCTTTTTTACATCTCGTCTATCTCAATTGGATAATGGGTCGGTGGCTTATGTTTTCTTTTGATTGTAATTTAATTGTACGCAACTGGACGCCAAAATGACAGGTTGAGTTTGATGAGTTTGAAGATAACTCAATGCTTGCCGCTACTGTTGTTTGGGACCATGCCTGTTCTTGGACTTGGGAAAATGCTGTTGAATCTCTTCAATCGACAATCGAACAGGTCTATTATCTGACACAATTGGATTGTCTCTGTTCATTGAATTGAAAGGAAGAATATTTTCAACCATAGATAATCCCTCCCTTGTGAATGTTTTACTATTCAGGTTTCTTCTAATAATGTAAATCTACAAAGAGATCTTCCACATATATCCATATTTAGCCACAATCACGTTCCCAGCAAAACATTCTGGGATCGTGCAATTGACAGGGCGCTGCAAGTGATTGAAAGCAACTCAGAACTCACCAAGGCACTCACCAGTTTTGGAATTATATCTTCCCTCTCACTCTCCCCTTCCCAAAATTCTTCACTCTTACCACAAATTTATTTATTCCTTGATTTTCATATCTTGTTTTCAATTTTTCTATTCACCATCAAAACTGGTTTGTCTAATCTTATGTATTATATTTAGGTCGTACTTGCACGTAGTACCCGAATAGTGACAAGCGTGGATGTTGATCCTGTTGCATGGGTAGCATGTTTACAGGTAGCTTTGTATGTTGCTATTCAGTTCTCTATGAATGTTACTTTTTATGCAGTAATTTTTTTCTAAATCTTGTTTTGATCGTATCAGAAAGAGGGGAAAAACTCTTATCAGTTTTGTGTCCAGCCATATAATGCCCCGGCCTTCATAGGAAGTACGGTATGTACGATTAATTATCTTGTAATAAAATTGTGTATGCTTCTTTGTATCAGTCTACCGTTTCATTTTGTTGCCTCATTCCAAGAACCTTCTTTGACAAAATGGAGCTTTGTTTGTTATAAAATGGGGAATGTTTTTGATACGGTGCTGAAGATGGGATATGCATGTAAGATGTTTGCAATTAGTTTCCGACCCGTTTGGCTTGTATATTCCCCATTTTCCCTCTATTTATTTATTTTCTTACTAATTTCTTTACTTTGATATGTTTGTAATAGCCTGAGAGACTGTTCCATAGAGAAGCACTTAGCATTAAAAGCGAGGCCTTAGCTGCAACCCGTGCTAGAGGCAGATCAGCTATTGAAGACCTTAAAATAGAACATGATTTATTTTCCAAGTTAGTTTCACCTCTAAATTCTTATTTTGTCTACTCAATGATGTTGTTACCCTGTTGCTTCAAACCAATGAGCTACGAGCAGGAGTTTCACTAACTATACTTTCTATGCGCAGCCCAAAGGATGACCTTGAGTTTTCTATTGTGAGGGAAACCATAAAAAGGAAGTTAGAGGCAAGTTTGATCTTCAGTCTTACTCTTTTGCAATCAAGCTTTCCGTGATGACATCAAATAGAACTTTTGCAGTGGCATGTTTTGGTTGTCCCTGATAGTCACCTGTCCTAATAAGTTTGGCACCTAGCAGCTATGTGCCACGAAAATGATATTCGCAGTGCTTCGATACAATGAATTTGTTCTCTTTTTTATCATTGTATTTGAAGCACTAAATAACATTTCTATACGTTTCTACATAAGTCGCCGAAATTTATAGGCAATAATGAAGAGGACAACATTTTGAGAATATCGCAGATTCTTAGCTAGCTTTATCCTGTTTGTAGGCTGTATGTTGTGACGCGGTAGTCGTTAAACCACATAAATCAGTTAGAAAACTTCCCAGAATTCAACATCTGTATGCCCAACTGACAGGCAGATTGAGAAGCGAGGAGGATGAGGTAAAAATCTCCAAAAATTTATGTCCCAGTAAATTAAACTTCCCTAACGAGAATAGACAGTTCTAACTCTACTTTGCTGCTTAAAAAGTTTGAAATTTTATCAACGCTTCATCCAACTCCCGCGGTTTGTGGATTGCCAACAGAGGAGGCTCGAGATCTAATTGCAGAAACTGGTAAGTGAATCTTGACGGTAAGATCCATGCATGGGGGTGTGATCCTTAACTTTTTCACGTATCAATGAAATTGAAAAGTTTGTTTCTTTTTCATAAGTGAACATACAAATAGGTTAGGTTAGTTTATTCTCTCTAAATTCCTAGAGGTCCTTTATATTATCTCCCCAGGCCCATCCAAGTTAGCGATGGTTTCCTTCGAAAGCTGATTATGGGTCTCGTAATTTTATCGTTTGCCAAATCTGGGATTGAAAGACAAGTCGTCTTTCACCCATCTGTATGAGTTTAAGAACTTGGGAAATTTTTTGGTGCAGAGGTATTTGATAGAGGGATGTACGCGGGGCCTGTTGGTTGGTTTGGAGGAGGAGAGAGTGAATTTGCTGTGGGCATCAGATCAGCACTAGTGCAGAGAGTAAGATGTCGTTTGCCATATGAGCTAAACAAATTTAAATACTTGGTTTAAATTATTACTCGATTTTAAAATTTCTAGTTCTGCTTAGACATCATTGCCATAACAATTCATTGAGTTCTAATAGTCTTTGATTTTAGGGTCTTGGTGCCTTGATCTATGCGGGAACAGGGATAGTAAAAGGAAGCAATCCGTCTTCGGAGTGGGATGAATTGGATCTCAAGATATCTCAGGTACTCTGGAAAGCATCTCCCTGGAACCTACATCTTTTGTCTTGGAAATGCTATTTAGTAGCTCAATTTTCGTTCCTTATAACTTTGATCTGCATGTTTGCAGGTCACCAGGTCGCTTAAACTAGAAGTGGCTCAACCACCCACAATTGGTTAGTGAAACTGTCGTCTGTGAAAGTAAGGGCTTAGGAAGCCATAAGGTGGGTTGCCTTAACTCCTCCAAATACGAATCTAATTCGAGAAACCAATAAGCTTGAAGAGTAACTAATTATTTAAGAACCCATAGATGGTTTTCTTAAATTCAAACTTCATATCATACATACTACATAGTTATCCTAAACTCAAGTTTTTTGACCCCAATTAAACTTTAGGCTTACTAAATTCAGGCGTACGATACCGGATTAGTGCACTCCAAACGCCCCCAAAATGGTTTTGAATTTTGATCATATAGCTTTGTTTAGAAAATGGCATCCTGGTAAGTTTCAATCTGACAGCTACTTATTTAAATATATGCGTTTTCCAGTGACAGACAGATACAACAAATAATGATTGCATAAGTTTGGATCTGAAGAAGTTCAGCGGGCATCCAGCAAGTGACACTCAGCCTCTACTTATAAGCTTTCTGTTCAGGTTCCTTCCGGGTTGTGGTTTGAGTCAGTAAATGGTTTCTCTGTTCTTCGTTTTGCTTTGGCTGTTTAG

mRNA sequence

ATGAGCATTTCCAAAATGGCTGTTTCTTCGACTCAAAATTCAGCCACCGGAGAGTGTGTTTGGTTCCGCCAGCATGTGAGTTGGCGGCGCCGCATTCCGGCAGTCGTAGCTTGGCCGAGGCGGTGGCGGAGGTGGGGCGGGGTGGTTTGCCAGTGGCCGGCGATGGATGGGTGCAGAGGAGACGAGAGAGGGGCCGTCGGGACGATTGAGACTCGCAGTTTTCCGGCGGTTGAGACGGCGGCTTTGGCGGCGGAGAGTCTGAGGGGAGAAGTGGCAAAGTTGAAGTCAGAGGCACCGCCAGTGAGTTCTGGAATTATTCGGCTACAAGTCCCAATACAACAGCACGTTCAAGCAATTGATTGGCTCTGTTCTCAGCCCCACCTTCTTCCTCGCACCTTCTTCTCCGGCAGAAGCAGAGCCATTGATCCTCATCCTCTCTCCGCCGACAACACTGCCGATGTCCGCCATCCCTTCAACGATCACCACCTGGTCGGCGTCGCCGGTGTGGGCTCTGCCGTCGTCTTCTGTCGCCTTCATCGATTTTCGAACCACGATTGGGCGTCTATAAAGAGGTTCCTCTCAAAAAAATGCCCCCTGATTCGTGCTTATGGAGGCATTCGCTTTGATTCACGGGCCAACATATCATCGGAGTGGGAGCCTTTTGGCTCGTTTTACTTCATGGTCCCTCAGGTTGAGTTTGATGAGTTTGAAGATAACTCAATGCTTGCCGCTACTGTTGTTTGGGACCATGCCTGTTCTTGGACTTGGGAAAATGCTGTTGAATCTCTTCAATCGACAATCGAACAGGTTTCTTCTAATAATGTAAATCTACAAAGAGATCTTCCACATATATCCATATTTAGCCACAATCACGTTCCCAGCAAAACATTCTGGGATCGTGCAATTGACAGGGCGCTGCAAGTCGTACTTGCACGTAGTACCCGAATAGTGACAAGCGTGGATGTTGATCCTGTTGCATGGGTAGCATGTTTACAGAAAGAGGGGAAAAACTCTTATCAGTTTTGTGTCCAGCCATATAATGCCCCGGCCTTCATAGGAAGTACGCCTGAGAGACTGTTCCATAGAGAAGCACTTAGCATTAAAAGCGAGGCCTTAGCTGCAACCCGTGCTAGAGGCAGATCAGCTATTGAAGACCTTAAAATAGAACATGATTTATTTTCCAACCCAAAGGATGACCTTGAGTTTTCTATTGTGAGGGAAACCATAAAAAGGAAGTTAGAGGCAATCGTTAAACCACATAAATCAGTTAGAAAACTTCCCAGAATTCAACATCTGTATGCCCAACTGACAGGCAGATTGAGAAGCGAGGAGGATGAGTTTGAAATTTTATCAACGCTTCATCCAACTCCCGCGGTTTGTGGATTGCCAACAGAGGAGGCTCGAGATCTAATTGCAGAAACTGAGGTATTTGATAGAGGGATGTACGCGGGGCCTGTTGGTTGGTTTGGAGGAGGAGAGAGTGAATTTGCTGTGGGCATCAGATCAGCACTAGTGCAGAGAGGTCTTGGTGCCTTGATCTATGCGGGAACAGGGATAGTAAAAGGAAGCAATCCGTCTTCGGAGTGGGATGAATTGGATCTCAAGATATCTCAGGTCACCAGGTCGCTTAAACTAGAAGTGGCTCAACCACCCACAATTGAAAATGGCATCCTGGTTCCTTCCGGGTTGTGGTTTGAGTCAGTAAATGGTTTCTCTGTTCTTCGTTTTGCTTTGGCTGTTTAG

Coding sequence (CDS)

ATGAGCATTTCCAAAATGGCTGTTTCTTCGACTCAAAATTCAGCCACCGGAGAGTGTGTTTGGTTCCGCCAGCATGTGAGTTGGCGGCGCCGCATTCCGGCAGTCGTAGCTTGGCCGAGGCGGTGGCGGAGGTGGGGCGGGGTGGTTTGCCAGTGGCCGGCGATGGATGGGTGCAGAGGAGACGAGAGAGGGGCCGTCGGGACGATTGAGACTCGCAGTTTTCCGGCGGTTGAGACGGCGGCTTTGGCGGCGGAGAGTCTGAGGGGAGAAGTGGCAAAGTTGAAGTCAGAGGCACCGCCAGTGAGTTCTGGAATTATTCGGCTACAAGTCCCAATACAACAGCACGTTCAAGCAATTGATTGGCTCTGTTCTCAGCCCCACCTTCTTCCTCGCACCTTCTTCTCCGGCAGAAGCAGAGCCATTGATCCTCATCCTCTCTCCGCCGACAACACTGCCGATGTCCGCCATCCCTTCAACGATCACCACCTGGTCGGCGTCGCCGGTGTGGGCTCTGCCGTCGTCTTCTGTCGCCTTCATCGATTTTCGAACCACGATTGGGCGTCTATAAAGAGGTTCCTCTCAAAAAAATGCCCCCTGATTCGTGCTTATGGAGGCATTCGCTTTGATTCACGGGCCAACATATCATCGGAGTGGGAGCCTTTTGGCTCGTTTTACTTCATGGTCCCTCAGGTTGAGTTTGATGAGTTTGAAGATAACTCAATGCTTGCCGCTACTGTTGTTTGGGACCATGCCTGTTCTTGGACTTGGGAAAATGCTGTTGAATCTCTTCAATCGACAATCGAACAGGTTTCTTCTAATAATGTAAATCTACAAAGAGATCTTCCACATATATCCATATTTAGCCACAATCACGTTCCCAGCAAAACATTCTGGGATCGTGCAATTGACAGGGCGCTGCAAGTCGTACTTGCACGTAGTACCCGAATAGTGACAAGCGTGGATGTTGATCCTGTTGCATGGGTAGCATGTTTACAGAAAGAGGGGAAAAACTCTTATCAGTTTTGTGTCCAGCCATATAATGCCCCGGCCTTCATAGGAAGTACGCCTGAGAGACTGTTCCATAGAGAAGCACTTAGCATTAAAAGCGAGGCCTTAGCTGCAACCCGTGCTAGAGGCAGATCAGCTATTGAAGACCTTAAAATAGAACATGATTTATTTTCCAACCCAAAGGATGACCTTGAGTTTTCTATTGTGAGGGAAACCATAAAAAGGAAGTTAGAGGCAATCGTTAAACCACATAAATCAGTTAGAAAACTTCCCAGAATTCAACATCTGTATGCCCAACTGACAGGCAGATTGAGAAGCGAGGAGGATGAGTTTGAAATTTTATCAACGCTTCATCCAACTCCCGCGGTTTGTGGATTGCCAACAGAGGAGGCTCGAGATCTAATTGCAGAAACTGAGGTATTTGATAGAGGGATGTACGCGGGGCCTGTTGGTTGGTTTGGAGGAGGAGAGAGTGAATTTGCTGTGGGCATCAGATCAGCACTAGTGCAGAGAGGTCTTGGTGCCTTGATCTATGCGGGAACAGGGATAGTAAAAGGAAGCAATCCGTCTTCGGAGTGGGATGAATTGGATCTCAAGATATCTCAGGTCACCAGGTCGCTTAAACTAGAAGTGGCTCAACCACCCACAATTGAAAATGGCATCCTGGTTCCTTCCGGGTTGTGGTTTGAGTCAGTAAATGGTTTCTCTGTTCTTCGTTTTGCTTTGGCTGTTTAG

Protein sequence

MSISKMAVSSTQNSATGECVWFRQHVSWRRRIPAVVAWPRRWRRWGGVVCQWPAMDGCRGDERGAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGIIRLQVPIQQHVQAIDWLCSQPHLLPRTFFSGRSRAIDPHPLSADNTADVRHPFNDHHLVGVAGVGSAVVFCRLHRFSNHDWASIKRFLSKKCPLIRAYGGIRFDSRANISSEWEPFGSFYFMVPQVEFDEFEDNSMLAATVVWDHACSWTWENAVESLQSTIEQVSSNNVNLQRDLPHISIFSHNHVPSKTFWDRAIDRALQVVLARSTRIVTSVDVDPVAWVACLQKEGKNSYQFCVQPYNAPAFIGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIEHDLFSNPKDDLEFSIVRETIKRKLEAIVKPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCGLPTEEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVQRGLGALIYAGTGIVKGSNPSSEWDELDLKISQVTRSLKLEVAQPPTIENGILVPSGLWFESVNGFSVLRFALAV
Homology
BLAST of Spg022055 vs. NCBI nr
Match: XP_038879640.1 (isochorismate synthase 2, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 915.2 bits (2364), Expect = 2.8e-262
Identity = 472/570 (82.81%), Postives = 498/570 (87.37%), Query Frame = 0

Query: 1   MSISKMAVSSTQNSATGECVWFRQHVSWRRRIPAVVAWPRRWRRW-GGVVCQWPAMDGCR 60
           MSISKM VS +QNSAT +CV F Q VS  RRIPA V WP R RRW  G VCQ  AMDGC 
Sbjct: 1   MSISKMTVSRSQNSATRQCVGFGQRVSRCRRIPAAVPWPGRGRRWVVGEVCQSAAMDGCG 60

Query: 61  GDERGAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGIIRLQVPIQQHVQAI 120
           GD  G VGTIETR+FPAV+TAALA ESLR  VAKLK EAP ++SGIIRLQVPIQQ +QAI
Sbjct: 61  GDGGGTVGTIETRTFPAVQTAALATESLRRGVAKLKLEAPALTSGIIRLQVPIQQRIQAI 120

Query: 121 DWLCSQPHLLPRTFFSG--RSRAIDPHPLSADNTADVRHPFNDHHLVGVAGVGSAVVFCR 180
           DWL SQPHLLPRTFFSG  RSRAID    SA NT++  HPFN HHL+G AGVGSAVVF R
Sbjct: 121 DWLSSQPHLLPRTFFSGRSRSRAID----SAHNTSNGSHPFNHHHLLGAAGVGSAVVFHR 180

Query: 181 LHRFSNHDWASIKRFLSKKCPLIRAYGGIRFDSRANISSEWEPFGSFYFMVPQVEFDEFE 240
           LH FS +DWASIKRFLSKKCPLIRAYG IRFDSRANIS EWEPFGSFYFMVPQVEFDEFE
Sbjct: 181 LHCFSYNDWASIKRFLSKKCPLIRAYGVIRFDSRANISPEWEPFGSFYFMVPQVEFDEFE 240

Query: 241 DNSMLAATVVWDHACSWTWENAVESLQSTIEQVSSNNVNLQRDLPHISIFSHNHVPSKTF 300
           +NSMLAATV WDHACSWTWENAVESLQST+EQVSSNNVNLQ+DLP I I S+ HVPSKTF
Sbjct: 241 ENSMLAATVAWDHACSWTWENAVESLQSTMEQVSSNNVNLQKDLPLIPIHSYTHVPSKTF 300

Query: 301 WDRAIDRALQ-----------VVLARSTRIVTSVDVDPVAWVACLQKEGKNSYQFCVQPY 360
           WDRA+DRALQ           VVLARSTRIVTSVD+DPV WVACLQKEGKNSYQFCVQP 
Sbjct: 301 WDRAVDRALQEIDRSNSELTKVVLARSTRIVTSVDIDPVTWVACLQKEGKNSYQFCVQPS 360

Query: 361 NAPAFIGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIEHDLFSNPKDDLEFSIVR 420
           NAPAFIG+TPERLFHRE L IKS+ALAATRARGRS IED+KIEHDLFSNPKDDLEFSIVR
Sbjct: 361 NAPAFIGNTPERLFHREGLGIKSDALAATRARGRSPIEDIKIEHDLFSNPKDDLEFSIVR 420

Query: 421 ETIKRKLEA-----IVKPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCG 480
           ETIK+KLEA     +V+PHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCG
Sbjct: 421 ETIKKKLEAVCDAVVVEPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCG 480

Query: 481 LPTEEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVQRGLGALIYAGTGIVK 540
           LPT EARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALV+RGLGALIYAGTGIVK
Sbjct: 481 LPTNEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVKRGLGALIYAGTGIVK 540

Query: 541 GSNPSSEWDELDLKISQVTRSLKLEVAQPP 552
           GSNPSSEWDEL+LKISQ+TRSLKLEVAQ P
Sbjct: 541 GSNPSSEWDELELKISQITRSLKLEVAQAP 566

BLAST of Spg022055 vs. NCBI nr
Match: XP_038879641.1 (isochorismate synthase 2, chloroplastic isoform X2 [Benincasa hispida])

HSP 1 Score: 888.6 bits (2295), Expect = 2.8e-254
Identity = 463/570 (81.23%), Postives = 489/570 (85.79%), Query Frame = 0

Query: 1   MSISKMAVSSTQNSATGECVWFRQHVSWRRRIPAVVAWPRRWRRW-GGVVCQWPAMDGCR 60
           MSISKM VS +QNSAT +CV F Q VS  RRIPA V WP R RRW  G VCQ  AMDGC 
Sbjct: 1   MSISKMTVSRSQNSATRQCVGFGQRVSRCRRIPAAVPWPGRGRRWVVGEVCQSAAMDGCG 60

Query: 61  GDERGAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGIIRLQVPIQQHVQAI 120
           GD  G VGTIETR+FPAV+TAALA ESLR  VAKLK EAP ++SGIIRLQVPIQQ +QAI
Sbjct: 61  GDGGGTVGTIETRTFPAVQTAALATESLRRGVAKLKLEAPALTSGIIRLQVPIQQRIQAI 120

Query: 121 DWLCSQPHLLPRTFFSG--RSRAIDPHPLSADNTADVRHPFNDHHLVGVAGVGSAVVFCR 180
           DWL SQPHLLPRTFFSG  RSRAID    SA NT++  HPFN HHL+G AGVGSAVVF R
Sbjct: 121 DWLSSQPHLLPRTFFSGRSRSRAID----SAHNTSNGSHPFNHHHLLGAAGVGSAVVFHR 180

Query: 181 LHRFSNHDWASIKRFLSKKCPLIRAYGGIRFDSRANISSEWEPFGSFYFMVPQVEFDEFE 240
           LH FS +DWASIKRFLSKKCPLIRAYG IRFDSRANIS EWEPFGSFYFMVPQVEFDEFE
Sbjct: 181 LHCFSYNDWASIKRFLSKKCPLIRAYGVIRFDSRANISPEWEPFGSFYFMVPQVEFDEFE 240

Query: 241 DNSMLAATVVWDHACSWTWENAVESLQSTIEQVSSNNVNLQRDLPHISIFSHNHVPSKTF 300
           +NSMLAATV WDHACSWTWENAVESLQST+EQVSSNNVNLQ+DLP I I S+ HVPSKTF
Sbjct: 241 ENSMLAATVAWDHACSWTWENAVESLQSTMEQVSSNNVNLQKDLPLIPIHSYTHVPSKTF 300

Query: 301 WDRAIDRALQ-----------VVLARSTRIVTSVDVDPVAWVACLQKEGKNSYQFCVQPY 360
           WDRA+DRALQ           VVLARSTRIVTSVD+DPV WVACLQKEGKNSYQFCVQP 
Sbjct: 301 WDRAVDRALQEIDRSNSELTKVVLARSTRIVTSVDIDPVTWVACLQKEGKNSYQFCVQPS 360

Query: 361 NAPAFIGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIEHDLFSNPKDDLEFSIVR 420
           NAPAFIG+TPERLFHRE L IKS+ALAATRARGRS IED+KIEHDLFSNPKDDLEFSIVR
Sbjct: 361 NAPAFIGNTPERLFHREGLGIKSDALAATRARGRSPIEDIKIEHDLFSNPKDDLEFSIVR 420

Query: 421 ETIKRKLEA-----IVKPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCG 480
           ETIK+KLEA     +V+PHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCG
Sbjct: 421 ETIKKKLEAVCDAVVVEPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCG 480

Query: 481 LPTEEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVQRGLGALIYAGTGIVK 540
           LPT EARDLIAET         GPVGWFGGGESEFAVGIRSALV+RGLGALIYAGTGIVK
Sbjct: 481 LPTNEARDLIAET---------GPVGWFGGGESEFAVGIRSALVKRGLGALIYAGTGIVK 540

Query: 541 GSNPSSEWDELDLKISQVTRSLKLEVAQPP 552
           GSNPSSEWDEL+LKISQ+TRSLKLEVAQ P
Sbjct: 541 GSNPSSEWDELELKISQITRSLKLEVAQAP 557

BLAST of Spg022055 vs. NCBI nr
Match: XP_022932292.1 (isochorismate synthase 2, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 875.2 bits (2260), Expect = 3.2e-250
Identity = 451/566 (79.68%), Postives = 483/566 (85.34%), Query Frame = 0

Query: 1   MSISKMAVSSTQNSATGECVWFRQHVSWRRRIPAVVAWPRRW-RRWGGVVCQWPAMDGCR 60
           MSI   A+SST  S TG CV F QH+S  RRIP +V +P RW RRWGGVVCQ PAMDGCR
Sbjct: 1   MSIPNKAISST--SPTGPCVGFAQHLSPGRRIPTLVIFPGRWRRRWGGVVCQSPAMDGCR 60

Query: 61  GDERGAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGIIRLQVPIQQHVQAI 120
            D  GAV T+ETR+FPAV T  LA E+LRG VAKLKSE P  SSGIIRLQ+PIQ+H++AI
Sbjct: 61  ADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLPIQRHIKAI 120

Query: 121 DWLCSQPHLLPRTFFSGRSRAIDPHPLSADNTADVRHPFNDHH-LVGVAGVGSAVVFCRL 180
           DWLCSQP LLPR FFSGRSRAID  PL A ++       N HH L  VAGVGSAVVFCR 
Sbjct: 121 DWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSS-------NAHHRLASVAGVGSAVVFCRH 180

Query: 181 HRFSNHDWASIKRFLSKKCPLIRAYGGIRFDSRANISSEWEPFGSFYFMVPQVEFDEFED 240
           H+FSN DWASIKR LSKKCPLIRAYGGIRFDSRANIS EWEPFGSFYFMVPQVEFDEFED
Sbjct: 181 HQFSNSDWASIKRLLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFED 240

Query: 241 NSMLAATVVWDHACSWTWENAVESLQSTIEQVSSNNVNLQRDLPHISIFSHNHVPSKTFW 300
           +S+LAATV WDHACSWTWENAVESLQSTIEQVSS+ VN+QRDLP ISI SHNHVP+KTFW
Sbjct: 241 DSILAATVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFW 300

Query: 301 DRAIDRALQ-----------VVLARSTRIVTSVDVDPVAWVACLQKEGKNSYQFCVQPYN 360
           D AI+RALQ           VVLARSTRIVTSVDVDP+ WVACLQKEGKNSYQFCVQP N
Sbjct: 301 DHAINRALQVIDQCDSDLTKVVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSN 360

Query: 361 APAFIGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIEHDLFSNPKDDLEFSIVRE 420
           APAFIG+TPERLFHRE LSI  EALAATRARGRSAIEDLKIE DL SNPKDDLEFSIVRE
Sbjct: 361 APAFIGNTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLSNPKDDLEFSIVRE 420

Query: 421 TIKRKLEA-----IVKPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCGL 480
            IKRKLEA     +V+PHKSVRKLPRIQHLYAQL GRLR+++DEFEILSTLHPTPAVCG 
Sbjct: 421 AIKRKLEAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGF 480

Query: 481 PTEEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVQRGLGALIYAGTGIVKG 540
           PTEEARDLIAETE+FDRGMYAGPVGWFGG ESEFAVGIRSALV+ GLGAL+YAGTGIVKG
Sbjct: 481 PTEEARDLIAETELFDRGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVKG 540

Query: 541 SNPSSEWDELDLKISQVTRSLKLEVA 549
           SNPS EWDEL+LKISQVTRSLKLE+A
Sbjct: 541 SNPSLEWDELELKISQVTRSLKLELA 557

BLAST of Spg022055 vs. NCBI nr
Match: XP_022972451.1 (isochorismate synthase 2, chloroplastic isoform X1 [Cucurbita maxima])

HSP 1 Score: 874.4 bits (2258), Expect = 5.5e-250
Identity = 450/567 (79.37%), Postives = 484/567 (85.36%), Query Frame = 0

Query: 1   MSISKMAVSST-QNSATGECVWFRQHVSWRRRIPAVVAWPRRW-RRWGGVVCQWPAMDGC 60
           MSI   A+SST Q S TG CV F QH+S  RRIP  V WP RW RRWGGVVCQ PAMDGC
Sbjct: 1   MSIPNKAISSTSQTSPTGPCVGFAQHLSPGRRIPTRVIWPGRWRRRWGGVVCQSPAMDGC 60

Query: 61  RGDERGAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGIIRLQVPIQQHVQA 120
           R D  GAV T+ETR+FPAV T  LA E+LRG VAKLKSE P  SSGIIRLQ+PIQQH++A
Sbjct: 61  RADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLPIQQHIKA 120

Query: 121 IDWLCSQPHLLPRTFFSGRSRAIDPHPLSADNTADVRHPFNDHH-LVGVAGVGSAVVFCR 180
           I WLCSQP LLPR FFSGRSRAID  PL + ++       N HH L  VAGVGSAVVFCR
Sbjct: 121 IHWLCSQPLLLPRIFFSGRSRAIDSDPLPSVSS-------NAHHRLASVAGVGSAVVFCR 180

Query: 181 LHRFSNHDWASIKRFLSKKCPLIRAYGGIRFDSRANISSEWEPFGSFYFMVPQVEFDEFE 240
            H+FSN DWASIKRFLSKKCPLIRA+GGIRFDSRANIS EWEPFGSFYFMVPQVEFDEFE
Sbjct: 181 HHQFSNSDWASIKRFLSKKCPLIRAFGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFE 240

Query: 241 DNSMLAATVVWDHACSWTWENAVESLQSTIEQVSSNNVNLQRDLPHISIFSHNHVPSKTF 300
           D+S+LAATV WDHACSWTWENAVESLQSTIEQVSS+NVN+QRDLP ISI SHNHVP+KTF
Sbjct: 241 DDSILAATVAWDHACSWTWENAVESLQSTIEQVSSDNVNVQRDLPQISILSHNHVPTKTF 300

Query: 301 WDRAIDRALQ-----------VVLARSTRIVTSVDVDPVAWVACLQKEGKNSYQFCVQPY 360
           WDRAI+RALQ           VVLARSTRIVTSV+VDPV WVACLQKEG++SYQFCVQP 
Sbjct: 301 WDRAINRALQAIDQCDSDLTKVVLARSTRIVTSVNVDPVTWVACLQKEGESSYQFCVQPA 360

Query: 361 NAPAFIGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIEHDLFSNPKDDLEFSIVR 420
           NAPAFIG+TPERLFHRE LSI  EALAATRARG+SAIEDLKIE DL SNPKDDLEFSIVR
Sbjct: 361 NAPAFIGNTPERLFHREGLSIICEALAATRARGKSAIEDLKIEQDLLSNPKDDLEFSIVR 420

Query: 421 ETIKRKLEA-----IVKPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCG 480
           E IKRKLEA     +V+PHKSVRKLPRIQHLYAQL GRLR+++DEFEILSTLHPTPAVCG
Sbjct: 421 EAIKRKLEAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCG 480

Query: 481 LPTEEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVQRGLGALIYAGTGIVK 540
            PTEEARDLIAETE+FDRGMYAGPVGWFGG ESEFAVGIRSALV+ G GAL+YAGTGIVK
Sbjct: 481 FPTEEARDLIAETELFDRGMYAGPVGWFGGEESEFAVGIRSALVETGHGALVYAGTGIVK 540

Query: 541 GSNPSSEWDELDLKISQVTRSLKLEVA 549
           GSNPS EWDEL+LKISQVTRSLKLE+A
Sbjct: 541 GSNPSLEWDELELKISQVTRSLKLELA 560

BLAST of Spg022055 vs. NCBI nr
Match: XP_023530897.1 (isochorismate synthase 2, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 866.3 bits (2237), Expect = 1.5e-247
Identity = 447/567 (78.84%), Postives = 480/567 (84.66%), Query Frame = 0

Query: 1   MSISKMAVSST-QNSATGECVWFRQHVSWRRRIPAVVAWPRRW-RRWGGVVCQWPAMDGC 60
           MSI   A+SST Q S TG CV F QH+S  RRIP +V  P RW RRWG VVCQ PAMDGC
Sbjct: 4   MSIPSKAISSTSQTSPTGPCVGFAQHLSPGRRIPTLVISPGRWRRRWGRVVCQSPAMDGC 63

Query: 61  RGDERGAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGIIRLQVPIQQHVQA 120
           R D  GAV T+ETR+FPAV T  LA E+LRG VAKLKSE P  SSGIIRLQ+PI +H++A
Sbjct: 64  RADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLPIHRHIKA 123

Query: 121 IDWLCSQPHLLPRTFFSGRSRAIDPHPLSADNTADVRHPFNDHHLV-GVAGVGSAVVFCR 180
           IDWLCSQP LLPR FFSGRSRAID   L + ++       N HH V  VAGVGSAVVFCR
Sbjct: 124 IDWLCSQPLLLPRIFFSGRSRAIDSDSLPSVSS-------NAHHRVASVAGVGSAVVFCR 183

Query: 181 LHRFSNHDWASIKRFLSKKCPLIRAYGGIRFDSRANISSEWEPFGSFYFMVPQVEFDEFE 240
            H+FSN DWASIKRFLSKKCPLIRAYGGIRFDSR NIS EWEPFGSFYFMVPQVEFDEFE
Sbjct: 184 HHQFSNSDWASIKRFLSKKCPLIRAYGGIRFDSRPNISPEWEPFGSFYFMVPQVEFDEFE 243

Query: 241 DNSMLAATVVWDHACSWTWENAVESLQSTIEQVSSNNVNLQRDLPHISIFSHNHVPSKTF 300
           D+S+LAATV WDHACSWTWENAVESLQST+EQVSS+NVN+QRDLP ISI SHNHVP+KTF
Sbjct: 244 DDSILAATVAWDHACSWTWENAVESLQSTVEQVSSDNVNVQRDLPQISILSHNHVPTKTF 303

Query: 301 WDRAIDRALQ-----------VVLARSTRIVTSVDVDPVAWVACLQKEGKNSYQFCVQPY 360
           WD AI+RALQ           VVLARSTRIVTSVDVDPV WVACLQKEGKNSYQFCVQP 
Sbjct: 304 WDSAINRALQVIDQCDSDLTKVVLARSTRIVTSVDVDPVTWVACLQKEGKNSYQFCVQPS 363

Query: 361 NAPAFIGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIEHDLFSNPKDDLEFSIVR 420
           NAPAFIG+TPERLFHRE LSI  EALAATRARG+SAIEDL+IE DL SNPKDDLEFSIVR
Sbjct: 364 NAPAFIGNTPERLFHREGLSIICEALAATRARGKSAIEDLRIEQDLLSNPKDDLEFSIVR 423

Query: 421 ETIKRKLEA-----IVKPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCG 480
           E IKRKLEA     +V+PHKSVRKLPRIQHLYAQL GRLR+ +DEFEILSTLHPTPAVCG
Sbjct: 424 EAIKRKLEAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTNDDEFEILSTLHPTPAVCG 483

Query: 481 LPTEEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVQRGLGALIYAGTGIVK 540
            PTEEARDLIAETE+FDRGMYAGPVGWFGG ESEFAVGIRSALV+ GLGAL+YAGTGIVK
Sbjct: 484 FPTEEARDLIAETELFDRGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVK 543

Query: 541 GSNPSSEWDELDLKISQVTRSLKLEVA 549
           GSNPS EWDEL+LKISQVTRSLKLE+A
Sbjct: 544 GSNPSLEWDELELKISQVTRSLKLELA 563

BLAST of Spg022055 vs. ExPASy Swiss-Prot
Match: Q9M9V6 (Isochorismate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ICS2 PE=2 SV=2)

HSP 1 Score: 597.0 bits (1538), Expect = 2.2e-169
Identity = 310/517 (59.96%), Postives = 386/517 (74.66%), Query Frame = 0

Query: 54  AMDGCRGDERGAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGIIRLQVPIQ 113
           +M+GC  D +  +GT+ETR+   V + A A E L   V+ LKS+ PP SSGI+RLQVPI+
Sbjct: 50  SMNGCEADHKAPLGTVETRTLSTVPSPAAATERLITAVSDLKSQPPPFSSGIVRLQVPIE 109

Query: 114 QHVQAIDWLCSQPHLLPRTFFSGRSRAIDPHPL---SADNTADVRHPFNDHHLVGVAGVG 173
           Q + AIDWL +Q  +LPR+FFS RS +  P  L   S+DN +      +DH+ V VAG+G
Sbjct: 110 QKIGAIDWLHAQNEILPRSFFSRRSDSGRPDLLQDFSSDNGS------SDHNPVSVAGIG 169

Query: 174 SAVVFCRLHRFSNHDWASIKRFLSKKCPLIRAYGGIRFDSRANISSEWEPFGSFYFMVPQ 233
           SAV F  L  FS+ DW SI+RFLS K PLIRAYGG+RFD    I+ EWE FGSFYF VPQ
Sbjct: 170 SAVFFRDLDPFSHDDWRSIRRFLSSKSPLIRAYGGLRFDPTGKIAVEWEHFGSFYFTVPQ 229

Query: 234 VEFDEFEDNSMLAATVVWDHACSWTWENAVESLQSTIEQVSSNNVNLQRDLPHISIFSHN 293
           VEFDEF  +SMLAATV WD+  SWT ENA+E+LQ T+ QVSS  + L+R+   + + S N
Sbjct: 230 VEFDEFGGSSMLAATVAWDNELSWTLENAIEALQETMLQVSSVIMRLRRESLGVIVVSKN 289

Query: 294 HVPSKTFWDRAIDRALQ-----------VVLARSTRIVTSVDVDPVAWVACLQKEGKNSY 353
           HVPS+  +  A++ AL+           VVLARS+RI+T  D+DP+AW+A LQ EG+++Y
Sbjct: 290 HVPSEGAYYPAVNNALEIIKDKHSPLSKVVLARSSRIITDTDIDPIAWLARLQCEGQDAY 349

Query: 354 QFCVQPYNAPAFIGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIEHDLFSNPKDD 413
           QFC+QP  APAFIG+TPERLFHR+ L + SEALAATR RG S + +++IE DL ++PKDD
Sbjct: 350 QFCLQPPGAPAFIGNTPERLFHRKHLGVCSEALAATRPRGDSKVREMEIERDLLTSPKDD 409

Query: 414 LEFSIVRETIKRKLEAI-----VKPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLH 473
           LEFSIVRE I+ KL+ I     VKPHKSVRKL R+QHLY+QL G+L+ E+DEF IL+ LH
Sbjct: 410 LEFSIVRENIREKLKTICDRVVVKPHKSVRKLARVQHLYSQLAGQLKREDDEFNILTALH 469

Query: 474 PTPAVCGLPTEEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVQRGLGALIY 533
           PTPAVCG P EEAR LI + E FDRGMYAGP+G+FGGGESEF+VGIRSALV++GLGALIY
Sbjct: 470 PTPAVCGCPVEEARLLIKQIESFDRGMYAGPIGFFGGGESEFSVGIRSALVEKGLGALIY 529

Query: 534 AGTGIVKGSNPSSEWDELDLKISQVTRSLKLEVAQPP 552
           AGTGIV GSNPSSEW+EL+LKISQ T+SL+ E A  P
Sbjct: 530 AGTGIVSGSNPSSEWNELELKISQFTKSLEHESALQP 560

BLAST of Spg022055 vs. ExPASy Swiss-Prot
Match: Q9ZPC0 (Isochorismate synthase, chloroplastic OS=Catharanthus roseus OX=4058 PE=1 SV=1)

HSP 1 Score: 574.7 bits (1480), Expect = 1.2e-162
Identity = 294/522 (56.32%), Postives = 384/522 (73.56%), Query Frame = 0

Query: 54  AMDGCRGDERGAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGIIRLQVPIQ 113
           +M+GC GD R  VGTIETR+ PAV T ALA E L   VA LKS  P   SGIIRL+VPI+
Sbjct: 54  SMNGCNGDPRAPVGTIETRTLPAVSTPALAMERLSSAVANLKSTLPSAQSGIIRLEVPIE 113

Query: 114 QHVQAIDWLCS--QPHLLPRTFFSGRSRAIDPHPLSADNT-----------ADVRHPFND 173
           +H++A+DWL S  Q +LLPR +FSGRS+       S D T             +    +D
Sbjct: 114 EHIEALDWLHSQDQKNLLPRCYFSGRSQVTFSDFTSNDLTNRNGSAANGHLQRISTSSDD 173

Query: 174 HHLVGVAGVGSAVVFCRLHRFSNHDWASIKRFLSKKCPLIRAYGGIRFDSRANISSEWEP 233
            +LV VAGVGSAV+F   + FS  DW SIKRFLSK CPLIRAYG IRFD+R +I+ EW+ 
Sbjct: 174 KNLVSVAGVGSAVLFRSPNPFSFDDWLSIKRFLSKNCPLIRAYGAIRFDARPHIAPEWKA 233

Query: 234 FGSFYFMVPQVEFDEFEDNSMLAATVVWDHACSWTWENAVESLQSTIEQVSSNNVNLQRD 293
           FGSFYF+VPQVEFDE   +SM+AATV WD+A S T++ A+ +LQ+T+EQVSS    L++D
Sbjct: 234 FGSFYFVVPQVEFDELHGSSMIAATVAWDNALSLTYQQAIVALQTTMEQVSSTVSKLRQD 293

Query: 294 LPHISIFSHNHVPSKTFWDRAIDRALQ-----------VVLARSTRIVTSVDVDPVAWVA 353
           + H S+ S  ++P +T WD  ++R L+           VVLAR ++++T+ D+DP+AW++
Sbjct: 294 VSHTSLVSKANIPDRTSWDLTLNRVLEEIGNKYSPLTKVVLARRSQVITTSDIDPLAWLS 353

Query: 354 CLQKEGKNSYQFCVQPYNAPAFIGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIE 413
             + +GK++YQFC+QP+ APAFIG+TPE+LF R+ L++ SEALAATRARG S   DL++ 
Sbjct: 354 SFKADGKDAYQFCLQPHEAPAFIGNTPEQLFGRDQLTVFSEALAATRARGESDSLDLQMA 413

Query: 414 HDLFSNPKDDLEFSIVRETIKRKLEAI-----VKPHKSVRKLPRIQHLYAQLTGRLRSEE 473
           HDLFS+PKD+ EF+IVRE I++KL+AI      +P KSVRKL RIQHLYA+  GRLRSE+
Sbjct: 414 HDLFSSPKDNHEFAIVRENIRQKLDAICTSVETEPMKSVRKLKRIQHLYARFAGRLRSED 473

Query: 474 DEFEILSTLHPTPAVCGLPTEEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSAL 533
           DEF+ILS+LHPTPAVCG P E+AR  IAE E+FDRG+YAGPVG+FGG +S+F+VGIRSAL
Sbjct: 474 DEFKILSSLHPTPAVCGFPMEDARKFIAENEMFDRGLYAGPVGFFGGAQSDFSVGIRSAL 533

Query: 534 VQRGLGALIYAGTGIVKGSNPSSEWDELDLKISQVTRSLKLE 547
           + +  GALIYAG G+V+GS+P+ EW EL+LK SQ  + +KLE
Sbjct: 534 IGKDAGALIYAGLGVVEGSDPALEWQELELKASQFMKLMKLE 575

BLAST of Spg022055 vs. ExPASy Swiss-Prot
Match: Q9S7H8 (Isochorismate synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ICS1 PE=1 SV=2)

HSP 1 Score: 572.0 bits (1473), Expect = 7.6e-162
Identity = 299/514 (58.17%), Postives = 372/514 (72.37%), Query Frame = 0

Query: 54  AMDGCRGDERGAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGIIRLQVPIQ 113
           +M+GC GD +  +GT+ETR+  AV + A A E L   V++LKS+ P  SSG++RLQVPI 
Sbjct: 47  SMNGCDGDFKTPLGTVETRTMTAVLSPAAATERLISAVSELKSQPPSFSSGVVRLQVPID 106

Query: 114 QHVQAIDWLCSQPHLLPRTFFSGRSRAIDPHPL-----SADNTADVRHPFNDHHLVGVAG 173
           Q + AIDWL +Q  + PR FFS RS    P  L        N        +D +LV VAG
Sbjct: 107 QQIGAIDWLQAQNEIQPRCFFSRRSDVGRPDLLLDLANENGNGNGNGTVSSDRNLVSVAG 166

Query: 174 VGSAVVFCRLHRFSNHDWASIKRFLSKKCPLIRAYGGIRFDSRANISSEWEPFGSFYFMV 233
           +GSAV F  L  FS+ DW SI+RFLS   PLIRAYGG+RFD    I+ EWEPFG+FYF V
Sbjct: 167 IGSAVFFRDLDPFSHDDWRSIRRFLSSTSPLIRAYGGMRFDPNGKIAVEWEPFGAFYFSV 226

Query: 234 PQVEFDEFEDNSMLAATVVWDHACSWTWENAVESLQSTIEQVSSNNVNLQRDLPHISIFS 293
           PQVEF+EF  +SMLAAT+ WD   SWT ENA+E+LQ T+ QVSS  + L+     +S+ S
Sbjct: 227 PQVEFNEFGGSSMLAATIAWDDELSWTLENAIEALQETMLQVSSVVMKLRNRSLGVSVLS 286

Query: 294 HNHVPSKTFWDRAIDRALQ-----------VVLARSTRIVTSVDVDPVAWVACLQKEGKN 353
            NHVP+K  +  A+++AL+           VVLAR++RI+T  D+DP+AW+A LQ+EG +
Sbjct: 287 KNHVPTKGAYFPAVEKALEMINQKSSPLNKVVLARNSRIITDTDIDPIAWLAQLQREGHD 346

Query: 354 SYQFCVQPYNAPAFIGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIEHDLFSNPK 413
           +YQFC+QP  APAFIG+TPERLF R  L + SEALAATR R  S+  D++IE DL ++PK
Sbjct: 347 AYQFCLQPPGAPAFIGNTPERLFQRTQLGVCSEALAATRPRAASSARDMEIERDLLTSPK 406

Query: 414 DDLEFSIVRETIKRKLEAI-----VKPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILST 473
           DDLEFSIVRE I+ KL  I     VKP K+VRKL R+QHLY+QL GRL  E+DE++IL+ 
Sbjct: 407 DDLEFSIVRENIREKLNGICDRVVVKPQKTVRKLARVQHLYSQLAGRLTKEDDEYKILAA 466

Query: 474 LHPTPAVCGLPTEEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVQRGLGAL 533
           LHPTPAVCGLP EEAR LI E E FDRGMYAGP+G+FGG ESEFAVGIRSALV++GLGAL
Sbjct: 467 LHPTPAVCGLPAEEARLLIKEIESFDRGMYAGPIGFFGGEESEFAVGIRSALVEKGLGAL 526

Query: 534 IYAGTGIVKGSNPSSEWDELDLKISQVTRSLKLE 547
           IYAGTGIV GS+PSSEW+ELDLKISQ T+S++ E
Sbjct: 527 IYAGTGIVAGSDPSSEWNELDLKISQFTKSIEYE 560

BLAST of Spg022055 vs. ExPASy Swiss-Prot
Match: P23973 (Isochorismate synthase MenF OS=Bacillus subtilis (strain 168) OX=224308 GN=menF PE=3 SV=2)

HSP 1 Score: 144.1 bits (362), Expect = 5.1e-33
Identity = 108/364 (29.67%), Postives = 172/364 (47.25%), Query Frame = 0

Query: 203 YGGIRFDSRANISSEWEPFGSFYFMVPQVEFDEFEDNSMLAATVVWDHACSWTWENAVES 262
           +GG  FD      S+W+ F    F VP +         ML  T          W +  E 
Sbjct: 119 FGGFSFDPCEERGSQWDHFSEGDFFVPAL---------MLTMTAEGPFLTVNRWVSGGED 178

Query: 263 LQSTIEQVSSNNVNL------QRDLPHISIFSHNHVPSKTFWDRAIDRAL---------Q 322
            ++ +E + +           Q D    ++ +      K  W +AI+ A          +
Sbjct: 179 AEAVLEGLKAFAAEFMVPDFKQEDQ---AVIAAAEELDKDDWLKAIETATSQIKEKQYDK 238

Query: 323 VVLARSTRIVTS--VDVDPVAWVACLQKEGKNSYQFCVQPYNAPAFIGSTPERLFHREAL 382
           VVLAR   +     + ++PV  +  L  + + SY F ++      F+G++PERL  R+  
Sbjct: 239 VVLARELLLTFDGPIQIEPV--LKTLLDDQQTSYVFAIE-QEGKTFVGASPERLIKRDGG 298

Query: 383 SIKSEALAATRARGRSAIEDLKIEHDLFSNPKDDLEFSIV----RETIKRKLEAIVKPHK 442
           ++ S  LA +  RG +  +D +I  +L ++ K+ LE  IV              + KP  
Sbjct: 299 TVMSSCLAGSIKRGVNEEDDRRIGLELLNDEKNLLEHDIVVGMIHNAFVSSCSEVEKPDG 358

Query: 443 SV-RKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCGLPTEEARDLIAETEVFDRG 502
            V  K   +QHL+  + G+LR     F+++  LHPTPA+ G P E+A D+I E E   RG
Sbjct: 359 PVLYKTKSVQHLFTPIVGQLRESASIFDLIEKLHPTPALGGSPQEKAVDVIREIEPMSRG 418

Query: 503 MYAGPVGWFGGGES-EFAVGIRSALVQRGLGALIYAGTGIVKGSNPSSEWDELDLKISQV 544
            YA P+GW    ++ EFAV IRS L++ G  A ++AG GIV+ S P SE++E  +K+  +
Sbjct: 419 WYAAPIGWIDSQDNGEFAVAIRSGLIE-GSTARLFAGCGIVEDSEPISEYEETQIKLKPM 466

BLAST of Spg022055 vs. ExPASy Swiss-Prot
Match: P38051 (Isochorismate synthase MenF OS=Escherichia coli (strain K12) OX=83333 GN=menF PE=1 SV=4)

HSP 1 Score: 138.3 bits (347), Expect = 2.8e-31
Identity = 95/279 (34.05%), Postives = 147/279 (52.69%), Query Frame = 0

Query: 283 HISIFSHNHVPSKTFWDRAIDRAL---------QVVLARSTRIVTSVDVDPVAWVACLQK 342
           H++     H P KT W + I+ A          +VVLAR+T +  +  V+  A +A  ++
Sbjct: 157 HLTTTREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRR 216

Query: 343 EGKNSYQFCVQPYNAPAFIGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIEHDLF 402
              N Y F +      AF+GS+PERL+ R   ++++EALA T A      +  ++   L 
Sbjct: 217 LNLNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM 276

Query: 403 SNPKDDLEFSIVRETIKRKLEA------IVKPHKSVRKLPRIQHLYAQLTGRLRSEEDEF 462
           ++ K+  E  +V E I ++L+A      ++ P   V +L ++QHL   +   L ++ D+ 
Sbjct: 277 ADDKNQRENMLVVEDICQRLQADTQTLDVLPP--QVLRLRKVQHLRRCIWTSL-NKADDV 336

Query: 463 EILSTLHPTPAVCGLPTEEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVQR 522
             L  L PT AV GLP + AR  IA  E F R  YAG  G+    +SEF V +RSA +  
Sbjct: 337 ICLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS- 396

Query: 523 GLGALIYAGTGIVKGSNPSSEWDELDLKISQVTRSLKLE 547
           G    +YAG GIV+GS+P  EW E+D K + +   L++E
Sbjct: 397 GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME 431

BLAST of Spg022055 vs. ExPASy TrEMBL
Match: A0A6J1EW89 (Isochorismate synthase OS=Cucurbita moschata OX=3662 GN=LOC111438650 PE=3 SV=1)

HSP 1 Score: 875.2 bits (2260), Expect = 1.6e-250
Identity = 451/566 (79.68%), Postives = 483/566 (85.34%), Query Frame = 0

Query: 1   MSISKMAVSSTQNSATGECVWFRQHVSWRRRIPAVVAWPRRW-RRWGGVVCQWPAMDGCR 60
           MSI   A+SST  S TG CV F QH+S  RRIP +V +P RW RRWGGVVCQ PAMDGCR
Sbjct: 1   MSIPNKAISST--SPTGPCVGFAQHLSPGRRIPTLVIFPGRWRRRWGGVVCQSPAMDGCR 60

Query: 61  GDERGAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGIIRLQVPIQQHVQAI 120
            D  GAV T+ETR+FPAV T  LA E+LRG VAKLKSE P  SSGIIRLQ+PIQ+H++AI
Sbjct: 61  ADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLPIQRHIKAI 120

Query: 121 DWLCSQPHLLPRTFFSGRSRAIDPHPLSADNTADVRHPFNDHH-LVGVAGVGSAVVFCRL 180
           DWLCSQP LLPR FFSGRSRAID  PL A ++       N HH L  VAGVGSAVVFCR 
Sbjct: 121 DWLCSQPLLLPRIFFSGRSRAIDSDPLPAVSS-------NAHHRLASVAGVGSAVVFCRH 180

Query: 181 HRFSNHDWASIKRFLSKKCPLIRAYGGIRFDSRANISSEWEPFGSFYFMVPQVEFDEFED 240
           H+FSN DWASIKR LSKKCPLIRAYGGIRFDSRANIS EWEPFGSFYFMVPQVEFDEFED
Sbjct: 181 HQFSNSDWASIKRLLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFED 240

Query: 241 NSMLAATVVWDHACSWTWENAVESLQSTIEQVSSNNVNLQRDLPHISIFSHNHVPSKTFW 300
           +S+LAATV WDHACSWTWENAVESLQSTIEQVSS+ VN+QRDLP ISI SHNHVP+KTFW
Sbjct: 241 DSILAATVAWDHACSWTWENAVESLQSTIEQVSSDYVNVQRDLPQISILSHNHVPTKTFW 300

Query: 301 DRAIDRALQ-----------VVLARSTRIVTSVDVDPVAWVACLQKEGKNSYQFCVQPYN 360
           D AI+RALQ           VVLARSTRIVTSVDVDP+ WVACLQKEGKNSYQFCVQP N
Sbjct: 301 DHAINRALQVIDQCDSDLTKVVLARSTRIVTSVDVDPITWVACLQKEGKNSYQFCVQPSN 360

Query: 361 APAFIGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIEHDLFSNPKDDLEFSIVRE 420
           APAFIG+TPERLFHRE LSI  EALAATRARGRSAIEDLKIE DL SNPKDDLEFSIVRE
Sbjct: 361 APAFIGNTPERLFHREGLSIICEALAATRARGRSAIEDLKIEQDLLSNPKDDLEFSIVRE 420

Query: 421 TIKRKLEA-----IVKPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCGL 480
            IKRKLEA     +V+PHKSVRKLPRIQHLYAQL GRLR+++DEFEILSTLHPTPAVCG 
Sbjct: 421 AIKRKLEAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGF 480

Query: 481 PTEEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVQRGLGALIYAGTGIVKG 540
           PTEEARDLIAETE+FDRGMYAGPVGWFGG ESEFAVGIRSALV+ GLGAL+YAGTGIVKG
Sbjct: 481 PTEEARDLIAETELFDRGMYAGPVGWFGGEESEFAVGIRSALVETGLGALVYAGTGIVKG 540

Query: 541 SNPSSEWDELDLKISQVTRSLKLEVA 549
           SNPS EWDEL+LKISQVTRSLKLE+A
Sbjct: 541 SNPSLEWDELELKISQVTRSLKLELA 557

BLAST of Spg022055 vs. ExPASy TrEMBL
Match: A0A6J1I605 (Isochorismate synthase OS=Cucurbita maxima OX=3661 GN=LOC111471007 PE=3 SV=1)

HSP 1 Score: 874.4 bits (2258), Expect = 2.6e-250
Identity = 450/567 (79.37%), Postives = 484/567 (85.36%), Query Frame = 0

Query: 1   MSISKMAVSST-QNSATGECVWFRQHVSWRRRIPAVVAWPRRW-RRWGGVVCQWPAMDGC 60
           MSI   A+SST Q S TG CV F QH+S  RRIP  V WP RW RRWGGVVCQ PAMDGC
Sbjct: 1   MSIPNKAISSTSQTSPTGPCVGFAQHLSPGRRIPTRVIWPGRWRRRWGGVVCQSPAMDGC 60

Query: 61  RGDERGAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGIIRLQVPIQQHVQA 120
           R D  GAV T+ETR+FPAV T  LA E+LRG VAKLKSE P  SSGIIRLQ+PIQQH++A
Sbjct: 61  RADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLPIQQHIKA 120

Query: 121 IDWLCSQPHLLPRTFFSGRSRAIDPHPLSADNTADVRHPFNDHH-LVGVAGVGSAVVFCR 180
           I WLCSQP LLPR FFSGRSRAID  PL + ++       N HH L  VAGVGSAVVFCR
Sbjct: 121 IHWLCSQPLLLPRIFFSGRSRAIDSDPLPSVSS-------NAHHRLASVAGVGSAVVFCR 180

Query: 181 LHRFSNHDWASIKRFLSKKCPLIRAYGGIRFDSRANISSEWEPFGSFYFMVPQVEFDEFE 240
            H+FSN DWASIKRFLSKKCPLIRA+GGIRFDSRANIS EWEPFGSFYFMVPQVEFDEFE
Sbjct: 181 HHQFSNSDWASIKRFLSKKCPLIRAFGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFE 240

Query: 241 DNSMLAATVVWDHACSWTWENAVESLQSTIEQVSSNNVNLQRDLPHISIFSHNHVPSKTF 300
           D+S+LAATV WDHACSWTWENAVESLQSTIEQVSS+NVN+QRDLP ISI SHNHVP+KTF
Sbjct: 241 DDSILAATVAWDHACSWTWENAVESLQSTIEQVSSDNVNVQRDLPQISILSHNHVPTKTF 300

Query: 301 WDRAIDRALQ-----------VVLARSTRIVTSVDVDPVAWVACLQKEGKNSYQFCVQPY 360
           WDRAI+RALQ           VVLARSTRIVTSV+VDPV WVACLQKEG++SYQFCVQP 
Sbjct: 301 WDRAINRALQAIDQCDSDLTKVVLARSTRIVTSVNVDPVTWVACLQKEGESSYQFCVQPA 360

Query: 361 NAPAFIGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIEHDLFSNPKDDLEFSIVR 420
           NAPAFIG+TPERLFHRE LSI  EALAATRARG+SAIEDLKIE DL SNPKDDLEFSIVR
Sbjct: 361 NAPAFIGNTPERLFHREGLSIICEALAATRARGKSAIEDLKIEQDLLSNPKDDLEFSIVR 420

Query: 421 ETIKRKLEA-----IVKPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCG 480
           E IKRKLEA     +V+PHKSVRKLPRIQHLYAQL GRLR+++DEFEILSTLHPTPAVCG
Sbjct: 421 EAIKRKLEAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCG 480

Query: 481 LPTEEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVQRGLGALIYAGTGIVK 540
            PTEEARDLIAETE+FDRGMYAGPVGWFGG ESEFAVGIRSALV+ G GAL+YAGTGIVK
Sbjct: 481 FPTEEARDLIAETELFDRGMYAGPVGWFGGEESEFAVGIRSALVETGHGALVYAGTGIVK 540

Query: 541 GSNPSSEWDELDLKISQVTRSLKLEVA 549
           GSNPS EWDEL+LKISQVTRSLKLE+A
Sbjct: 541 GSNPSLEWDELELKISQVTRSLKLELA 560

BLAST of Spg022055 vs. ExPASy TrEMBL
Match: A0A0A0LS91 (Isochorismate synthase OS=Cucumis sativus OX=3659 GN=Csa_1G008580 PE=3 SV=1)

HSP 1 Score: 862.8 bits (2228), Expect = 8.0e-247
Identity = 444/565 (78.58%), Postives = 479/565 (84.78%), Query Frame = 0

Query: 8   VSSTQNSATG-ECVWFRQHVSWRRRIPAVVAWPRRWRRWGGVVCQWPAMDGCRGDER--- 67
           +S   NSATG  C+ F Q +S  RRI   VAW  R RRWGG VC   AMDGC G  +   
Sbjct: 1   MSIANNSATGRHCLGFHQRLSRCRRISPAVAWRERGRRWGGEVCDSAAMDGCVGGSQEPG 60

Query: 68  GAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGIIRLQVPIQQHVQAIDWLC 127
           G VG IETR+FPAV+TAALA E LR  VA+LKSEAP  +SGIIRLQVPIQQ +QAIDWL 
Sbjct: 61  GTVGIIETRNFPAVQTAALATERLRRGVAELKSEAPAFTSGIIRLQVPIQQRIQAIDWLS 120

Query: 128 SQPHLLPRTFFSGRSRAIDPHPLSADNTADVRHPFN-DHHLVGVAGVGSAVVFCRLHRFS 187
           SQPHLLPRTFFSGRSR   P   SA N ++  H FN  HHL+ VAGVGSAVVF RLH FS
Sbjct: 121 SQPHLLPRTFFSGRSR---PTHSSAHNISNGSHSFNHHHHLLSVAGVGSAVVFRRLHCFS 180

Query: 188 NHDWASIKRFLSKKCPLIRAYGGIRFDSRANISSEWEPFGSFYFMVPQVEFDEFEDNSML 247
            +DWASIKRFLSKKCPLIRAYG IRFDSRANIS EWEPFGSFYFMVPQVEFDEFE+NSML
Sbjct: 181 YNDWASIKRFLSKKCPLIRAYGVIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEENSML 240

Query: 248 AATVVWDHACSWTWENAVESLQSTIEQVSSNNVNLQRDLPHISIFSHNHVPSKTFWDRAI 307
           AATV WDHACSWTWENA+ESLQSTIEQVSSNNVNLQ+DLP I I S+ HVPSKTFW+RA+
Sbjct: 241 AATVAWDHACSWTWENAIESLQSTIEQVSSNNVNLQKDLPQIPILSYTHVPSKTFWERAV 300

Query: 308 DRALQ-----------VVLARSTRIVTSVDVDPVAWVACLQKEGKNSYQFCVQPYNAPAF 367
           DRALQ           VVLARST+IVT VD+DPV WVACLQKEG NSYQFCVQP NAPAF
Sbjct: 301 DRALQVIDGSNSELTKVVLARSTQIVTGVDIDPVTWVACLQKEGNNSYQFCVQPSNAPAF 360

Query: 368 IGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIEHDLFSNPKDDLEFSIVRETIKR 427
           IG+TPERLFHRE L IKS+ALAATRARGR+AIED+KIEHDLFSNPK+DLEFSIVRETIK+
Sbjct: 361 IGNTPERLFHREGLGIKSDALAATRARGRTAIEDIKIEHDLFSNPKEDLEFSIVRETIKK 420

Query: 428 KLEA-----IVKPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCGLPTEE 487
           KLEA     +V+P+KSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCGLPTEE
Sbjct: 421 KLEAVCDAVVVEPYKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCGLPTEE 480

Query: 488 ARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVQRGLGALIYAGTGIVKGSNPS 547
           ARD IAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALV+RGLGALIYAGTGIVKGSNPS
Sbjct: 481 ARDFIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVKRGLGALIYAGTGIVKGSNPS 540

Query: 548 SEWDELDLKISQVTRSLKLEVAQPP 552
           SEWDEL+LKISQ++RSL+LEV   P
Sbjct: 541 SEWDELELKISQISRSLELEVVHAP 562

BLAST of Spg022055 vs. ExPASy TrEMBL
Match: A0A5D3BJQ7 (Isochorismate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G002380 PE=3 SV=1)

HSP 1 Score: 861.3 bits (2224), Expect = 2.3e-246
Identity = 445/570 (78.07%), Postives = 479/570 (84.04%), Query Frame = 0

Query: 3   ISKMAVSSTQNSAT-GECVWFRQHVSWRRRIPAVVAWPRRWRRWGGVVCQWPAMDGCRG- 62
           ++ +AVSS QNSAT    + F Q  S  RRI A VAWP R RRWG  VC   AMDGC G 
Sbjct: 4   LNNVAVSSNQNSATCRHFIGFHQRGSRCRRISAAVAWPERGRRWGEEVCDSAAMDGCGGG 63

Query: 63  ---DERGAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGIIRLQVPIQQHVQ 122
              +  G VGTIETR+FPAV TAALA ESLR  VA+LKSEAP +SSGIIRLQVPIQQ +Q
Sbjct: 64  NNQEAGGTVGTIETRNFPAVRTAALATESLRRGVAELKSEAPALSSGIIRLQVPIQQRIQ 123

Query: 123 AIDWLCSQPHLLPRTFFSGRSRAIDPHPLSADNTADVRHPFNDHHLVGVAGVGSAVVFCR 182
           AIDWL SQPHLLPRTFFSGRSR  D    SA N ++  H FN  HL+GVAGVGSAVVF R
Sbjct: 124 AIDWLSSQPHLLPRTFFSGRSRPTDS---SAHNISNGSHSFNHRHLLGVAGVGSAVVFRR 183

Query: 183 LHRFSNHDWASIKRFLSKKCPLIRAYGGIRFDSRANISSEWEPFGSFYFMVPQVEFDEFE 242
           LH FS +DWASIKRFLSKKCPLIRAYGGIRFDSRANIS EWEPFGSFYFMVPQVEFDEFE
Sbjct: 184 LHCFSYNDWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFE 243

Query: 243 DNSMLAATVVWDHACSWTWENAVESLQSTIEQVSSNNVNLQRDLPHISIFSHNHVPSKTF 302
           +NSMLAATV WDHACSWTWENAVESLQSTIEQVSSNNVNLQ+D P I I S+ HVP KTF
Sbjct: 244 ENSMLAATVAWDHACSWTWENAVESLQSTIEQVSSNNVNLQKDPPQIPILSYTHVPGKTF 303

Query: 303 WDRAIDRALQ-----------VVLARSTRIVTSVDVDPVAWVACLQKEGKNSYQFCVQPY 362
           W+RA+DRALQ           VVLARST+IVT VD+DPV  +AC QKEG NSYQFCVQP 
Sbjct: 304 WERAVDRALQEIDGSNSELTKVVLARSTQIVTGVDIDPVTCIACFQKEGNNSYQFCVQPS 363

Query: 363 NAPAFIGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIEHDLFSNPKDDLEFSIVR 422
           NA AFIG+TPERLFHRE L IKS+ALAATRARGRSAIED+KIEHDLFSNPK+DLEFSIVR
Sbjct: 364 NAHAFIGNTPERLFHREGLDIKSDALAATRARGRSAIEDIKIEHDLFSNPKEDLEFSIVR 423

Query: 423 ETIKRKLEA-----IVKPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCG 482
           E IK+KLEA     +V+P+KSVRKLPRIQHLYAQLTGRLRSEEDEFEILS LHPTPAVCG
Sbjct: 424 EAIKKKLEAVCDAVVVEPYKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSMLHPTPAVCG 483

Query: 483 LPTEEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVQRGLGALIYAGTGIVK 542
            PTEEARD IAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALV+RGLGALIYAGTGIVK
Sbjct: 484 WPTEEARDFIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVKRGLGALIYAGTGIVK 543

Query: 543 GSNPSSEWDELDLKISQVTRSLKLEVAQPP 552
           GSNPSSEWDEL+LKISQ++RSL+LEV Q P
Sbjct: 544 GSNPSSEWDELELKISQISRSLELEVVQEP 570

BLAST of Spg022055 vs. ExPASy TrEMBL
Match: A0A1S3BV15 (Isochorismate synthase OS=Cucumis melo OX=3656 GN=LOC103493974 PE=3 SV=1)

HSP 1 Score: 861.3 bits (2224), Expect = 2.3e-246
Identity = 445/570 (78.07%), Postives = 479/570 (84.04%), Query Frame = 0

Query: 3   ISKMAVSSTQNSAT-GECVWFRQHVSWRRRIPAVVAWPRRWRRWGGVVCQWPAMDGCRG- 62
           ++ +AVSS QNSAT    + F Q  S  RRI A VAWP R RRWG  VC   AMDGC G 
Sbjct: 4   LNNVAVSSNQNSATCRHFIGFHQRGSRCRRISAAVAWPERGRRWGEKVCDSAAMDGCGGG 63

Query: 63  ---DERGAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGIIRLQVPIQQHVQ 122
              +  G VGTIETR+FPAV TAALA ESLR  VA+LKSEAP +SSGIIRLQVPIQQ +Q
Sbjct: 64  NNQEAGGTVGTIETRNFPAVRTAALATESLRRGVAELKSEAPALSSGIIRLQVPIQQRIQ 123

Query: 123 AIDWLCSQPHLLPRTFFSGRSRAIDPHPLSADNTADVRHPFNDHHLVGVAGVGSAVVFCR 182
           AIDWL SQPHLLPRTFFSGRSR  D    SA N ++  H FN  HL+GVAGVGSAVVF R
Sbjct: 124 AIDWLSSQPHLLPRTFFSGRSRPTDS---SAHNISNGSHSFNHRHLLGVAGVGSAVVFRR 183

Query: 183 LHRFSNHDWASIKRFLSKKCPLIRAYGGIRFDSRANISSEWEPFGSFYFMVPQVEFDEFE 242
           LH FS +DWASIKRFLSKKCPLIRAYGGIRFDSRANIS EWEPFGSFYFMVPQVEFDEFE
Sbjct: 184 LHCFSYNDWASIKRFLSKKCPLIRAYGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFE 243

Query: 243 DNSMLAATVVWDHACSWTWENAVESLQSTIEQVSSNNVNLQRDLPHISIFSHNHVPSKTF 302
           +NSMLAATV WDHACSWTWENAVESLQSTIEQVSSNNVNLQ+D P I I S+ HVP KTF
Sbjct: 244 ENSMLAATVAWDHACSWTWENAVESLQSTIEQVSSNNVNLQKDPPQIPILSYTHVPGKTF 303

Query: 303 WDRAIDRALQ-----------VVLARSTRIVTSVDVDPVAWVACLQKEGKNSYQFCVQPY 362
           W+RA+DRALQ           VVLARST+IVT VD+DPV  +AC QKEG NSYQFCVQP 
Sbjct: 304 WERAVDRALQEIDGSNSELTKVVLARSTQIVTGVDIDPVTCIACFQKEGNNSYQFCVQPS 363

Query: 363 NAPAFIGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIEHDLFSNPKDDLEFSIVR 422
           NA AFIG+TPERLFHRE L IKS+ALAATRARGRSAIED+KIEHDLFSNPK+DLEFSIVR
Sbjct: 364 NAHAFIGNTPERLFHREGLDIKSDALAATRARGRSAIEDIKIEHDLFSNPKEDLEFSIVR 423

Query: 423 ETIKRKLEA-----IVKPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHPTPAVCG 482
           E IK+KLEA     +V+P+KSVRKLPRIQHLYAQLTGRLRSEEDEFEILS LHPTPAVCG
Sbjct: 424 EAIKKKLEAVCDAVVVEPYKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSMLHPTPAVCG 483

Query: 483 LPTEEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVQRGLGALIYAGTGIVK 542
            PTEEARD IAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALV+RGLGALIYAGTGIVK
Sbjct: 484 WPTEEARDFIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVKRGLGALIYAGTGIVK 543

Query: 543 GSNPSSEWDELDLKISQVTRSLKLEVAQPP 552
           GSNPSSEWDEL+LKISQ++RSL+LEV Q P
Sbjct: 544 GSNPSSEWDELELKISQISRSLELEVVQEP 570

BLAST of Spg022055 vs. TAIR 10
Match: AT1G18870.1 (isochorismate synthase 2 )

HSP 1 Score: 597.0 bits (1538), Expect = 1.6e-170
Identity = 310/517 (59.96%), Postives = 386/517 (74.66%), Query Frame = 0

Query: 54  AMDGCRGDERGAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGIIRLQVPIQ 113
           +M+GC  D +  +GT+ETR+   V + A A E L   V+ LKS+ PP SSGI+RLQVPI+
Sbjct: 50  SMNGCEADHKAPLGTVETRTLSTVPSPAAATERLITAVSDLKSQPPPFSSGIVRLQVPIE 109

Query: 114 QHVQAIDWLCSQPHLLPRTFFSGRSRAIDPHPL---SADNTADVRHPFNDHHLVGVAGVG 173
           Q + AIDWL +Q  +LPR+FFS RS +  P  L   S+DN +      +DH+ V VAG+G
Sbjct: 110 QKIGAIDWLHAQNEILPRSFFSRRSDSGRPDLLQDFSSDNGS------SDHNPVSVAGIG 169

Query: 174 SAVVFCRLHRFSNHDWASIKRFLSKKCPLIRAYGGIRFDSRANISSEWEPFGSFYFMVPQ 233
           SAV F  L  FS+ DW SI+RFLS K PLIRAYGG+RFD    I+ EWE FGSFYF VPQ
Sbjct: 170 SAVFFRDLDPFSHDDWRSIRRFLSSKSPLIRAYGGLRFDPTGKIAVEWEHFGSFYFTVPQ 229

Query: 234 VEFDEFEDNSMLAATVVWDHACSWTWENAVESLQSTIEQVSSNNVNLQRDLPHISIFSHN 293
           VEFDEF  +SMLAATV WD+  SWT ENA+E+LQ T+ QVSS  + L+R+   + + S N
Sbjct: 230 VEFDEFGGSSMLAATVAWDNELSWTLENAIEALQETMLQVSSVIMRLRRESLGVIVVSKN 289

Query: 294 HVPSKTFWDRAIDRALQ-----------VVLARSTRIVTSVDVDPVAWVACLQKEGKNSY 353
           HVPS+  +  A++ AL+           VVLARS+RI+T  D+DP+AW+A LQ EG+++Y
Sbjct: 290 HVPSEGAYYPAVNNALEIIKDKHSPLSKVVLARSSRIITDTDIDPIAWLARLQCEGQDAY 349

Query: 354 QFCVQPYNAPAFIGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIEHDLFSNPKDD 413
           QFC+QP  APAFIG+TPERLFHR+ L + SEALAATR RG S + +++IE DL ++PKDD
Sbjct: 350 QFCLQPPGAPAFIGNTPERLFHRKHLGVCSEALAATRPRGDSKVREMEIERDLLTSPKDD 409

Query: 414 LEFSIVRETIKRKLEAI-----VKPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLH 473
           LEFSIVRE I+ KL+ I     VKPHKSVRKL R+QHLY+QL G+L+ E+DEF IL+ LH
Sbjct: 410 LEFSIVRENIREKLKTICDRVVVKPHKSVRKLARVQHLYSQLAGQLKREDDEFNILTALH 469

Query: 474 PTPAVCGLPTEEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVQRGLGALIY 533
           PTPAVCG P EEAR LI + E FDRGMYAGP+G+FGGGESEF+VGIRSALV++GLGALIY
Sbjct: 470 PTPAVCGCPVEEARLLIKQIESFDRGMYAGPIGFFGGGESEFSVGIRSALVEKGLGALIY 529

Query: 534 AGTGIVKGSNPSSEWDELDLKISQVTRSLKLEVAQPP 552
           AGTGIV GSNPSSEW+EL+LKISQ T+SL+ E A  P
Sbjct: 530 AGTGIVSGSNPSSEWNELELKISQFTKSLEHESALQP 560

BLAST of Spg022055 vs. TAIR 10
Match: AT1G18870.2 (isochorismate synthase 2 )

HSP 1 Score: 596.7 bits (1537), Expect = 2.0e-170
Identity = 310/516 (60.08%), Postives = 385/516 (74.61%), Query Frame = 0

Query: 55  MDGCRGDERGAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGIIRLQVPIQQ 114
           M+GC  D +  +GT+ETR+   V + A A E L   V+ LKS+ PP SSGI+RLQVPI+Q
Sbjct: 1   MNGCEADHKAPLGTVETRTLSTVPSPAAATERLITAVSDLKSQPPPFSSGIVRLQVPIEQ 60

Query: 115 HVQAIDWLCSQPHLLPRTFFSGRSRAIDPHPL---SADNTADVRHPFNDHHLVGVAGVGS 174
            + AIDWL +Q  +LPR+FFS RS +  P  L   S+DN +      +DH+ V VAG+GS
Sbjct: 61  KIGAIDWLHAQNEILPRSFFSRRSDSGRPDLLQDFSSDNGS------SDHNPVSVAGIGS 120

Query: 175 AVVFCRLHRFSNHDWASIKRFLSKKCPLIRAYGGIRFDSRANISSEWEPFGSFYFMVPQV 234
           AV F  L  FS+ DW SI+RFLS K PLIRAYGG+RFD    I+ EWE FGSFYF VPQV
Sbjct: 121 AVFFRDLDPFSHDDWRSIRRFLSSKSPLIRAYGGLRFDPTGKIAVEWEHFGSFYFTVPQV 180

Query: 235 EFDEFEDNSMLAATVVWDHACSWTWENAVESLQSTIEQVSSNNVNLQRDLPHISIFSHNH 294
           EFDEF  +SMLAATV WD+  SWT ENA+E+LQ T+ QVSS  + L+R+   + + S NH
Sbjct: 181 EFDEFGGSSMLAATVAWDNELSWTLENAIEALQETMLQVSSVIMRLRRESLGVIVVSKNH 240

Query: 295 VPSKTFWDRAIDRALQ-----------VVLARSTRIVTSVDVDPVAWVACLQKEGKNSYQ 354
           VPS+  +  A++ AL+           VVLARS+RI+T  D+DP+AW+A LQ EG+++YQ
Sbjct: 241 VPSEGAYYPAVNNALEIIKDKHSPLSKVVLARSSRIITDTDIDPIAWLARLQCEGQDAYQ 300

Query: 355 FCVQPYNAPAFIGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIEHDLFSNPKDDL 414
           FC+QP  APAFIG+TPERLFHR+ L + SEALAATR RG S + +++IE DL ++PKDDL
Sbjct: 301 FCLQPPGAPAFIGNTPERLFHRKHLGVCSEALAATRPRGDSKVREMEIERDLLTSPKDDL 360

Query: 415 EFSIVRETIKRKLEAI-----VKPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILSTLHP 474
           EFSIVRE I+ KL+ I     VKPHKSVRKL R+QHLY+QL G+L+ E+DEF IL+ LHP
Sbjct: 361 EFSIVRENIREKLKTICDRVVVKPHKSVRKLARVQHLYSQLAGQLKREDDEFNILTALHP 420

Query: 475 TPAVCGLPTEEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVQRGLGALIYA 534
           TPAVCG P EEAR LI + E FDRGMYAGP+G+FGGGESEF+VGIRSALV++GLGALIYA
Sbjct: 421 TPAVCGCPVEEARLLIKQIESFDRGMYAGPIGFFGGGESEFSVGIRSALVEKGLGALIYA 480

Query: 535 GTGIVKGSNPSSEWDELDLKISQVTRSLKLEVAQPP 552
           GTGIV GSNPSSEW+EL+LKISQ T+SL+ E A  P
Sbjct: 481 GTGIVSGSNPSSEWNELELKISQFTKSLEHESALQP 510

BLAST of Spg022055 vs. TAIR 10
Match: AT1G74710.1 (ADC synthase superfamily protein )

HSP 1 Score: 572.0 bits (1473), Expect = 5.4e-163
Identity = 299/514 (58.17%), Postives = 372/514 (72.37%), Query Frame = 0

Query: 54  AMDGCRGDERGAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGIIRLQVPIQ 113
           +M+GC GD +  +GT+ETR+  AV + A A E L   V++LKS+ P  SSG++RLQVPI 
Sbjct: 47  SMNGCDGDFKTPLGTVETRTMTAVLSPAAATERLISAVSELKSQPPSFSSGVVRLQVPID 106

Query: 114 QHVQAIDWLCSQPHLLPRTFFSGRSRAIDPHPL-----SADNTADVRHPFNDHHLVGVAG 173
           Q + AIDWL +Q  + PR FFS RS    P  L        N        +D +LV VAG
Sbjct: 107 QQIGAIDWLQAQNEIQPRCFFSRRSDVGRPDLLLDLANENGNGNGNGTVSSDRNLVSVAG 166

Query: 174 VGSAVVFCRLHRFSNHDWASIKRFLSKKCPLIRAYGGIRFDSRANISSEWEPFGSFYFMV 233
           +GSAV F  L  FS+ DW SI+RFLS   PLIRAYGG+RFD    I+ EWEPFG+FYF V
Sbjct: 167 IGSAVFFRDLDPFSHDDWRSIRRFLSSTSPLIRAYGGMRFDPNGKIAVEWEPFGAFYFSV 226

Query: 234 PQVEFDEFEDNSMLAATVVWDHACSWTWENAVESLQSTIEQVSSNNVNLQRDLPHISIFS 293
           PQVEF+EF  +SMLAAT+ WD   SWT ENA+E+LQ T+ QVSS  + L+     +S+ S
Sbjct: 227 PQVEFNEFGGSSMLAATIAWDDELSWTLENAIEALQETMLQVSSVVMKLRNRSLGVSVLS 286

Query: 294 HNHVPSKTFWDRAIDRALQ-----------VVLARSTRIVTSVDVDPVAWVACLQKEGKN 353
            NHVP+K  +  A+++AL+           VVLAR++RI+T  D+DP+AW+A LQ+EG +
Sbjct: 287 KNHVPTKGAYFPAVEKALEMINQKSSPLNKVVLARNSRIITDTDIDPIAWLAQLQREGHD 346

Query: 354 SYQFCVQPYNAPAFIGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIEHDLFSNPK 413
           +YQFC+QP  APAFIG+TPERLF R  L + SEALAATR R  S+  D++IE DL ++PK
Sbjct: 347 AYQFCLQPPGAPAFIGNTPERLFQRTQLGVCSEALAATRPRAASSARDMEIERDLLTSPK 406

Query: 414 DDLEFSIVRETIKRKLEAI-----VKPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILST 473
           DDLEFSIVRE I+ KL  I     VKP K+VRKL R+QHLY+QL GRL  E+DE++IL+ 
Sbjct: 407 DDLEFSIVRENIREKLNGICDRVVVKPQKTVRKLARVQHLYSQLAGRLTKEDDEYKILAA 466

Query: 474 LHPTPAVCGLPTEEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVQRGLGAL 533
           LHPTPAVCGLP EEAR LI E E FDRGMYAGP+G+FGG ESEFAVGIRSALV++GLGAL
Sbjct: 467 LHPTPAVCGLPAEEARLLIKEIESFDRGMYAGPIGFFGGEESEFAVGIRSALVEKGLGAL 526

Query: 534 IYAGTGIVKGSNPSSEWDELDLKISQVTRSLKLE 547
           IYAGTGIV GS+PSSEW+ELDLKISQ T+S++ E
Sbjct: 527 IYAGTGIVAGSDPSSEWNELDLKISQFTKSIEYE 560

BLAST of Spg022055 vs. TAIR 10
Match: AT1G74710.2 (ADC synthase superfamily protein )

HSP 1 Score: 567.0 bits (1460), Expect = 1.7e-161
Identity = 297/507 (58.58%), Postives = 367/507 (72.39%), Query Frame = 0

Query: 54  AMDGCRGDERGAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGIIRLQVPIQ 113
           +M+GC GD +  +GT+ETR+  AV + A A E L   V++LKS+ P  SSG++RLQVPI 
Sbjct: 47  SMNGCDGDFKTPLGTVETRTMTAVLSPAAATERLISAVSELKSQPPSFSSGVVRLQVPID 106

Query: 114 QHVQAIDWLCSQPHLLPRTFFSGRSRAIDPHPL-----SADNTADVRHPFNDHHLVGVAG 173
           Q + AIDWL +Q  + PR FFS RS    P  L        N        +D +LV VAG
Sbjct: 107 QQIGAIDWLQAQNEIQPRCFFSRRSDVGRPDLLLDLANENGNGNGNGTVSSDRNLVSVAG 166

Query: 174 VGSAVVFCRLHRFSNHDWASIKRFLSKKCPLIRAYGGIRFDSRANISSEWEPFGSFYFMV 233
           +GSAV F  L  FS+ DW SI+RFLS   PLIRAYGG+RFD    I+ EWEPFG+FYF V
Sbjct: 167 IGSAVFFRDLDPFSHDDWRSIRRFLSSTSPLIRAYGGMRFDPNGKIAVEWEPFGAFYFSV 226

Query: 234 PQVEFDEFEDNSMLAATVVWDHACSWTWENAVESLQSTIEQVSSNNVNLQRDLPHISIFS 293
           PQVEF+EF  +SMLAAT+ WD   SWT ENA+E+LQ T+ QVSS  + L+     +S+ S
Sbjct: 227 PQVEFNEFGGSSMLAATIAWDDELSWTLENAIEALQETMLQVSSVVMKLRNRSLGVSVLS 286

Query: 294 HNHVPSKTFWDRAIDRALQ-----------VVLARSTRIVTSVDVDPVAWVACLQKEGKN 353
            NHVP+K  +  A+++AL+           VVLAR++RI+T  D+DP+AW+A LQ+EG +
Sbjct: 287 KNHVPTKGAYFPAVEKALEMINQKSSPLNKVVLARNSRIITDTDIDPIAWLAQLQREGHD 346

Query: 354 SYQFCVQPYNAPAFIGSTPERLFHREALSIKSEALAATRARGRSAIEDLKIEHDLFSNPK 413
           +YQFC+QP  APAFIG+TPERLF R  L + SEALAATR R  S+  D++IE DL ++PK
Sbjct: 347 AYQFCLQPPGAPAFIGNTPERLFQRTQLGVCSEALAATRPRAASSARDMEIERDLLTSPK 406

Query: 414 DDLEFSIVRETIKRKLEAI-----VKPHKSVRKLPRIQHLYAQLTGRLRSEEDEFEILST 473
           DDLEFSIVRE I+ KL  I     VKP K+VRKL R+QHLY+QL GRL  E+DE++IL+ 
Sbjct: 407 DDLEFSIVRENIREKLNGICDRVVVKPQKTVRKLARVQHLYSQLAGRLTKEDDEYKILAA 466

Query: 474 LHPTPAVCGLPTEEARDLIAETEVFDRGMYAGPVGWFGGGESEFAVGIRSALVQRGLGAL 533
           LHPTPAVCGLP EEAR LI E E FDRGMYAGP+G+FGG ESEFAVGIRSALV++GLGAL
Sbjct: 467 LHPTPAVCGLPAEEARLLIKEIESFDRGMYAGPIGFFGGEESEFAVGIRSALVEKGLGAL 526

Query: 534 IYAGTGIVKGSNPSSEWDELDLKISQV 540
           IYAGTGIV GS+PSSEW+ELDLKISQV
Sbjct: 527 IYAGTGIVAGSDPSSEWNELDLKISQV 553

BLAST of Spg022055 vs. TAIR 10
Match: AT1G68890.1 (magnesium ion binding;thiamin pyrophosphate binding;hydro-lyases;catalytics;2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthases )

HSP 1 Score: 114.0 bits (284), Expect = 4.0e-25
Identity = 78/233 (33.48%), Postives = 119/233 (51.07%), Query Frame = 0

Query: 47  GVVCQWPAMD-GCRGDERGAVGTIETRSFPAVETAALAAESLRGEVAKLKSEAPPVSSGI 106
           GV    P MD     D+   V    TR+ P   T  L  ESL+  + +LK+  P  SSG+
Sbjct: 56  GVQFDGPIMDRDVNLDDDLVVQVCVTRTLPPALTLELGLESLKEAIDELKTNPPKSSSGV 115

Query: 107 IRLQVPIQQHVQAIDWLCSQP---HLLPRTFFSGRSRAIDPHPLSADNTADVRHPFNDHH 166
           +R QV +    +A+ W CSQP    + P  F S     ++P    +  +  V+ P     
Sbjct: 116 LRFQVAVPPRAKALFWFCSQPTTSDVFPVFFLS--KDTVEP----SYKSLYVKEPH---- 175

Query: 167 LVGVAGVGSAVVFCRLHRFSNHDWASIKRFLSKKCPLIRAYG--GIRFDSRANISSEWEP 226
             GV G+G+A  F       ++  + IK FLS +  ++ AYG   I F+  + ++S+   
Sbjct: 176 --GVFGIGNAFAFVHSSSVDSNGHSMIKTFLSDESAMVTAYGFPDIEFNKYSTVNSK--- 235

Query: 227 FGSFYFMVPQVEFDEFEDNSMLAATVVWDHACSWTWENAVESLQSTIEQVSSN 274
            GS YF VPQ+E DE E+ S+LA T+ W+ + S+T E  + S + +I QVSS+
Sbjct: 236 DGSSYFFVPQIELDEHEEVSILAVTLAWNESLSYTVEQTISSYEKSIFQVSSH 273

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038879640.12.8e-26282.81isochorismate synthase 2, chloroplastic isoform X1 [Benincasa hispida][more]
XP_038879641.12.8e-25481.23isochorismate synthase 2, chloroplastic isoform X2 [Benincasa hispida][more]
XP_022932292.13.2e-25079.68isochorismate synthase 2, chloroplastic-like isoform X1 [Cucurbita moschata][more]
XP_022972451.15.5e-25079.37isochorismate synthase 2, chloroplastic isoform X1 [Cucurbita maxima][more]
XP_023530897.11.5e-24778.84isochorismate synthase 2, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9M9V62.2e-16959.96Isochorismate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ICS2 ... [more]
Q9ZPC01.2e-16256.32Isochorismate synthase, chloroplastic OS=Catharanthus roseus OX=4058 PE=1 SV=1[more]
Q9S7H87.6e-16258.17Isochorismate synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ICS1 ... [more]
P239735.1e-3329.67Isochorismate synthase MenF OS=Bacillus subtilis (strain 168) OX=224308 GN=menF ... [more]
P380512.8e-3134.05Isochorismate synthase MenF OS=Escherichia coli (strain K12) OX=83333 GN=menF PE... [more]
Match NameE-valueIdentityDescription
A0A6J1EW891.6e-25079.68Isochorismate synthase OS=Cucurbita moschata OX=3662 GN=LOC111438650 PE=3 SV=1[more]
A0A6J1I6052.6e-25079.37Isochorismate synthase OS=Cucurbita maxima OX=3661 GN=LOC111471007 PE=3 SV=1[more]
A0A0A0LS918.0e-24778.58Isochorismate synthase OS=Cucumis sativus OX=3659 GN=Csa_1G008580 PE=3 SV=1[more]
A0A5D3BJQ72.3e-24678.07Isochorismate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3... [more]
A0A1S3BV152.3e-24678.07Isochorismate synthase OS=Cucumis melo OX=3656 GN=LOC103493974 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G18870.11.6e-17059.96isochorismate synthase 2 [more]
AT1G18870.22.0e-17060.08isochorismate synthase 2 [more]
AT1G74710.15.4e-16358.17ADC synthase superfamily protein [more]
AT1G74710.21.7e-16158.58ADC synthase superfamily protein [more]
AT1G68890.14.0e-2533.48magnesium ion binding;thiamin pyrophosphate binding;hydro-lyases;catalytics;2-su... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 260..280
NoneNo IPR availableCOILSCoilCoilcoord: 77..97
NoneNo IPR availablePANTHERPTHR47253:SF4ISOCHORISMATE SYNTHASE 2, CHLOROPLASTICcoord: 54..549
IPR015890Chorismate-utilising enzyme, C-terminalPFAMPF00425Chorismate_bindcoord: 306..535
e-value: 5.0E-55
score: 186.8
IPR005801ADC synthaseGENE3D3.60.120.10Anthranilate synthasecoord: 83..545
e-value: 4.2E-94
score: 318.0
IPR005801ADC synthaseSUPERFAMILY56322ADC synthasecoord: 147..545
IPR004561Isochorismate synthaseTIGRFAMTIGR00543TIGR00543coord: 201..543
e-value: 4.9E-95
score: 316.6
IPR044250Isochorismate synthase MenF-likePANTHERPTHR47253FAMILY NOT NAMEDcoord: 54..549

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg022055.1Spg022055.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042372 phylloquinone biosynthetic process
biological_process GO:0009058 biosynthetic process
cellular_component GO:0009536 plastid
molecular_function GO:0008909 isochorismate synthase activity