Spg022032 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg022032
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionRibonuclease H domain
Locationscaffold2: 7264036 .. 7268264 (+)
RNA-Seq ExpressionSpg022032
SyntenySpg022032
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAAAGCGGCCTTGCTGGTTTTTACAGGACACGTTTCATGGAAGTCTCATAAAGCTGCCAGGTGGTAAAACTTACACAACACTTTGACCGACAATAATTGGGTTACTAATTGGGTTCAGACTCAGACACATAGCCAATCTCGACTTTTCCCTTCTTCTCTTGTTCTCTCCGGTCGCTGTCTCCTCCCTCGTTTGCCTGCGATCACGAAGCCGCCGCCGCCTGGGGGTTTTGCTTGCCGGCGACCTGCTGCTACGAAGTCTCGCTCCGACCGCTGTCTCCTCCCTCGTTTGCCTGCGATCACGAAGCCGCCGCCGCCGCCTGGGGATTTTGCTCGCCGACGACCTGCTGCTACGAAGTCTCGCCGCTGCGTCCAGTGCTCTCGTCTGGACTGTTCTGACCGCGTTTCAGCCGGCGCCGCCGTGGACCGTTGTCGTTTGGTTAAGTTCCGGCGAGTTTTATTCCTTTAAGCCACTGCCGAGAAGTAAGTGCTAGGGGCCGCTGTGAATCTTGTGTGGTGGGACTAAATGGCCGATGGATTTCACGATTTGACGGTGCCTCCACCCTACTTAAATATAAATATCATTAATCATTAATTAGGGCAAAAGGGTAAAGTAGGCATTATTTAAAATGTTAGGGGCAATAAGGGAATGGAAAAATTCTCCCCTCTAAGCCCCTTTTATATATAGTAGAGATTATACTATACATATTTTAATTTAACAAAAATAGATTGAGTTCATAACATGAAATATTACATTTATTAAAGTTTTTTTTTATCTTTTTTAATTTAATTTTCATTTTAAAATTTTAAATTCAAAATCTGGATACACTGAAAATATTAAAAAAAAAAGTTGTTTTCAAAATTCCTACTATTTGGATCACAAAAATCGAAAACAATTTTCAAAACACATTTGCCAAGTATATTAAAATTTATATGTGAATTGCATTACAAACAGGTCCTTAGGATTTTATTAGACACAAAAAAATTTATATCTAATAGATCAATTCATTTTTAAAAAATTTTGAATTTGTCTGGACCTATTAGACACAAAAATGAAAGTTCAAGAATTTTATTAAATACAAAATTGAAAGTTTAGAAATTTATTAGACACTCTTGAAAGTTAAGGGATCAAATGGACATAAACTTTAAATTTCAAAGATTAAGGGCCCGTTTGATAACCATTTCAGTTTTTGGTGTTTGGTTTTTCTGTTTTAGGTTTTTGGTTTTTGGTTTCCTGTTTTTTAAAAACAAAAACCACATATGTTTGATAACTGTTTTTTGTTTTCTATTTTTAAAAAACAGAAAATCAACCTTGTTTGATAACTGTTTTTGGTTTTCAGTTTTTTAAAAATTAGAAATATGAAAAGATATTTTAGAGAGAGAAAAATGTGTAAAGACGTTGAAACAAAAATTTAAAGACTCAAATATAAATGTTTTCAAGAGATTTTTTTTTTTGAAAAGACGAGAGAAAAGTTAAACATAGAAAATTTAGAGAGATGAAAGAAAATGTTTAGAGAAAGAATATTAGTTAGACTCGAAAAAAGATATTAGTTAGAGAAAAACATAGAAAACAAGTTAGAGTAAAAGCGATAAATTTTAGATTAAAAAAATTAAATAAAAAAAGAAAATTTAGAAAGATAGTAAAGTTAAGAGTAAAAGATATGCTAAAGAACTAACGATAAATTAGAAAAATAAAGAAAAATTGTAGCAATATAAAATTTTGGTGATTTTTTTTTTCAAAAGAAAAATTAGAACATGTTTATTGAGGAGAGAAAATGTAATGACAAGTTTTTAGAAATAGACAAGTTATAGAGGAAAAAAAAGTTTTGAGAGAAAAATAAATTTTAGAGAGAGAAAAAAAATAGAGAAAGAAATTTGTTGGAGAGAGAGGTAAAAGAGGAGAAAAGTAAGCATTAGAAGAAAAATGAGTTTTTGAAAAACAGAAATCTGTTTTTAAAAACTATTTTTTTTGGTTTTTGAAATTTACTTATAATTTCAAAAACGTTTTTAAAAAACAGAAAACAGAAAAAAGAAATGGTTATCAAACACCCCTGTTTTTTAAAAATGAAAAATAAAAACAGAAAACAGAAAACGAAATGGTTATCAAACGGGCCCTAAACTTTTAATTTAACTTTCTTTGTTTTAGGAATGACCACGTAAGTTGACCATATTATAGTTTGTGTAGTCACATGTTAATTTTTTTTAGAATAAGTTTATAATTTATAAAAGTTAATATATAGCCAAAGTGAGCTTAGCTCAGTGGTAAATGACATGTACCTTCGATCAAGAAGTCAGAGGTTCAAATACCCCATCCCTAAATGTTGATGTAGTAAAAAAAAAAAAGTTAATATATAAGTATAAAATGAGAGCTTTATAAATTTGAATCAATGTAATAAGTTTACAAAATAGTTAAATATATGTTTTAGTCTTGATGTTGGATTATTTTTTTAATTTAATCTTTTATGTTTTGATATTTTTCAAGTATATTCTTCATAAATGTTAAAATTGAGAGGTGATAAATTAACTTTGATATAATATTTAGTGAATTGACATAAATTTAGATAAGATGCCCTAGAAGACGAGAGTTTATATTTTCTCTTAAAATTTATAAAAAATTTAGTAGAAATTATTTTTGAGTTTTGATTTTTTTTTTTATTAATTTTTGTAATCTTTGTGATGATATAACTCTGCGTTTGAATTTCTACCCATTTGTCATTTCATGTCATGTACATTTATCATCGATTAATTTTGGTATTTACCAACAAGAAGAGTAATAGTATAATAGAAAATGAATTAAAATATATAAAATGAAGTTAAATTTTTTTAAACATTAGAAATTAAATTGAAAAAATACCAAAAAACTATTATAGACTAAAACATTTAGTCTACCAAAAATGTTAAAAGATTGAGCTTAAAACAAATAAATGCACATATTAAATATAAACACTTGATTTCTTAATCCCTTGAGTTTAAACCTAACTTTAGTGCCAAACATTTTTTTAAATAACTTCTATACCAACAAATTAGGAGTGTTCTTTTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGCTCGGGGCAGAAATTGAAATTAGTTATTGCTAAATGGTAGTTAGTATTTAATTATTATATTTTAAAATATTACCTTCATGTACGGTGAAAAGATTTAAATTTATGAGACATAAATATTTTAACAACTAAATTAATTATGTTATATTTGTAGATTAATGTTTATTACTTAGTTAACGGGCACGTAGCAACTTCAACATTTCTCTGTTAACCATACTACTTACCTTATTTGAGTAATTTATGAGAGTGGAGATTGTTTTTACAGATCACCACACAAGTCCTTTCAACATGTTTTATTCTCAATCACATATTTCCTAAGAAATTTCCTACGATGTCACTTAGCATAGAATTGTTTCAAGTTAAGCACATTTAACTTTAGAGCTTTTATGATTGAATTACCCAAAAATGAAAGTGCACTTTATCGGTATTATAAGTAGTAACTATTAATTTTATAAACAGTCGTTTTAGCTTTCATAATCTCAATCTTTCATTCTATTGAGTGTCTACTGATTGATGTACTACTCCTCCACTCAAGTGTCACACATCTATTAAGTTAATTCGAAGAAATACCTGGAAGCAAATTCGAAAAACCGTGATTCAGAAGCTGTAATCCGTCAATCGGGCCAACATGCACTTGGTAGATGTTGGTCCCCTCCCCCTGTTGATCACTGGAAATTTAATGTCGACGCGGCCTGGTCTTCTCCGATGCCCTCGACTGGAATAGGAGTTATTGGGAGAAAGTCGGATGGGAGTACTGGAGGTATATCTATGAAACATCTTGACGGAAATTTCGAAGGGCTTGGAGCTGAGTTAATCGCAATCAAGGTGGCTTTGGAGCTGGCGGTACAGATGAGGTGTCATAAGATTATCGTGGAGTCTGATTCTGAGTTGGCTATTAAGTTCATTAATAAATCCTTAGACGTCTGGAGTGAGGTGGAATGTACTGTGGTGGCTATTTGGGATCTTATTCCATTTTTTTCAGAAGTGGTGTTTAATTTCATTCCTAGAAATGTCAATAGAGTAGCAGATAAGCTGGCTAAGTGGGCTAAAATGTCTAAATCTGATGGTATCTGGGTAGAGGATTTTTCAGCTTGGATTGTATCGATGGTCGAGGATGACCGTTTTCTCTGTGGCCATGTGGCGTCTTAA

mRNA sequence

ATGGAAAAGCGGCCTTGCTGCCAATCTCGACTTTTCCCTTCTTCTCTTGTTCTCTCCGGTCGCTGTCTCCTCCCTCGTTTGCCTGCGATCACGAAGCCGCCGCCGCCTGGGGGTTTTGCTTGCCGGCGACCTGCTGCTACGAAGTCTCGCTCCGACCGCTGTCTCCTCCCTCGTTTGCCTGCGATCACGAAGCCGCCGCCGCCGCCTGGGGATTTTGCTCGCCGACGACCTGCTGCTACGAAGTCTCGCCGCTGCGTCCAGTGCTCTCGTCTGGACTGTTCTGACCGCGTTTCAGCCGGCGCCGCCGTGGACCGTTGTCGTTTGAAATACCTGGAAGCAAATTCGAAAAACCGTGATTCAGAAGCTGTAATCCGTCAATCGGGCCAACATGCACTTGGTAGATGTTGGTCCCCTCCCCCTGTTGATCACTGGAAATTTAATGTCGACGCGGCCTGGTCTTCTCCGATGCCCTCGACTGGAATAGGAGTTATTGGGAGAAAGTCGGATGGGAGTACTGGAGGTATATCTATGAAACATCTTGACGGAAATTTCGAAGGGCTTGGAGCTGAGTTAATCGCAATCAAGGTGGCTTTGGAGCTGGCGGTACAGATGAGGTGTCATAAGATTATCGTGGAGTCTGATTCTGAGTTGGCTATTAAGTTCATTAATAAATCCTTAGACGTCTGGAGTGAGGTGGAATGTACTGTGGTGGCTATTTGGGATCTTATTCCATTTTTTTCAGAAGTGGTGTTTAATTTCATTCCTAGAAATGTCAATAGAGTAGCAGATAAGCTGGCTAAGTGGGCTAAAATGTCTAAATCTGATGGTATCTGGGTAGAGGATTTTTCAGCTTGGATTGTATCGATGGTCGAGGATGACCGTTTTCTCTGTGGCCATGTGGCGTCTTAA

Coding sequence (CDS)

ATGGAAAAGCGGCCTTGCTGCCAATCTCGACTTTTCCCTTCTTCTCTTGTTCTCTCCGGTCGCTGTCTCCTCCCTCGTTTGCCTGCGATCACGAAGCCGCCGCCGCCTGGGGGTTTTGCTTGCCGGCGACCTGCTGCTACGAAGTCTCGCTCCGACCGCTGTCTCCTCCCTCGTTTGCCTGCGATCACGAAGCCGCCGCCGCCGCCTGGGGATTTTGCTCGCCGACGACCTGCTGCTACGAAGTCTCGCCGCTGCGTCCAGTGCTCTCGTCTGGACTGTTCTGACCGCGTTTCAGCCGGCGCCGCCGTGGACCGTTGTCGTTTGAAATACCTGGAAGCAAATTCGAAAAACCGTGATTCAGAAGCTGTAATCCGTCAATCGGGCCAACATGCACTTGGTAGATGTTGGTCCCCTCCCCCTGTTGATCACTGGAAATTTAATGTCGACGCGGCCTGGTCTTCTCCGATGCCCTCGACTGGAATAGGAGTTATTGGGAGAAAGTCGGATGGGAGTACTGGAGGTATATCTATGAAACATCTTGACGGAAATTTCGAAGGGCTTGGAGCTGAGTTAATCGCAATCAAGGTGGCTTTGGAGCTGGCGGTACAGATGAGGTGTCATAAGATTATCGTGGAGTCTGATTCTGAGTTGGCTATTAAGTTCATTAATAAATCCTTAGACGTCTGGAGTGAGGTGGAATGTACTGTGGTGGCTATTTGGGATCTTATTCCATTTTTTTCAGAAGTGGTGTTTAATTTCATTCCTAGAAATGTCAATAGAGTAGCAGATAAGCTGGCTAAGTGGGCTAAAATGTCTAAATCTGATGGTATCTGGGTAGAGGATTTTTCAGCTTGGATTGTATCGATGGTCGAGGATGACCGTTTTCTCTGTGGCCATGTGGCGTCTTAA

Protein sequence

MEKRPCCQSRLFPSSLVLSGRCLLPRLPAITKPPPPGGFACRRPAATKSRSDRCLLPRLPAITKPPPPPGDFARRRPAATKSRRCVQCSRLDCSDRVSAGAAVDRCRLKYLEANSKNRDSEAVIRQSGQHALGRCWSPPPVDHWKFNVDAAWSSPMPSTGIGVIGRKSDGSTGGISMKHLDGNFEGLGAELIAIKVALELAVQMRCHKIIVESDSELAIKFINKSLDVWSEVECTVVAIWDLIPFFSEVVFNFIPRNVNRVADKLAKWAKMSKSDGIWVEDFSAWIVSMVEDDRFLCGHVAS
Homology
BLAST of Spg022032 vs. NCBI nr
Match: XP_038902513.1 (uncharacterized protein LOC120089172 [Benincasa hispida])

HSP 1 Score: 87.4 bits (215), Expect = 2.3e-13
Identity = 51/150 (34.00%), Postives = 76/150 (50.67%), Query Frame = 0

Query: 134 RCWSPPPVDHWKFNVDAAWSSPMPSTGIGVIGRKSDGSTGGISMKHLDGNFEGLGAELIA 193
           R WS PP    K NVDAAW     S+G   I R + GS   + +  +D  +    AE   
Sbjct: 54  RTWSTPPTSFIKLNVDAAWKFSPYSSGFSAIIRDNQGSLKVVQISSIDAVYPPPLAEAFV 113

Query: 194 IKVALELAVQMRCHKIIVESDSELAIKFINKSLDVWSEVECTVVAIWDLIPFFSEVVFNF 253
           +   L L  +M   KIIV+SD   AI    K L   S V   +  IW++   F  + F +
Sbjct: 114 VLQGLRLTSKMNFKKIIVKSDCSGAIDLFLKILISDSSVRVWLEEIWEISIVFYPINFAY 173

Query: 254 IPRNVNRVADKLAKWAKMSKSDGIWVEDFS 284
           IPRN+N++AD +AK  ++   + +W++ +S
Sbjct: 174 IPRNINKLADIVAKRTRILGINDVWMDSYS 203

BLAST of Spg022032 vs. NCBI nr
Match: OVA10548.1 (Ribonuclease H domain [Macleaya cordata])

HSP 1 Score: 84.3 bits (207), Expect = 1.9e-12
Identity = 48/165 (29.09%), Postives = 87/165 (52.73%), Query Frame = 0

Query: 136 WSPPPVDHWKFNVDAAWSSPMPSTGIGVIGRKSDGSTGGISMKHLDGNFEGLGAELIAIK 195
           WSPP    +K N D AW       G+G++ R S G     +  H++  +    AE+ AI 
Sbjct: 322 WSPPRSGWFKINTDGAWDPNTSKGGVGLVTRDSFGLFLSAAAMHIN-TYSAEEAEIRAIW 381

Query: 196 VALELAVQMRCHKIIVESDSELAIKFINKS--LDVW-SEVECTVVAIWDLIPFFSEVVFN 255
            A++ A++ +   + +ESD+++ ++ + +      W ++     + +W L   F ++ FN
Sbjct: 382 TAMKQAMERKITALEIESDAKVVVEQLKRKNYCGAWNTDALLKDIEVWSL--SFEKLSFN 441

Query: 256 FIPRNVNRVADKLAKWAKMSKSDGIWVEDFSAWIVSMVEDDRFLC 298
           ++PR  NRVA +LA+W K S +D  W      W++ ++  D+FLC
Sbjct: 442 YVPRVCNRVAHELAQWGKNSTTDMFWGTP-PVWLLPVLRGDQFLC 482

BLAST of Spg022032 vs. NCBI nr
Match: OVA19644.1 (Ribonuclease H domain [Macleaya cordata])

HSP 1 Score: 84.3 bits (207), Expect = 1.9e-12
Identity = 48/165 (29.09%), Postives = 87/165 (52.73%), Query Frame = 0

Query: 136 WSPPPVDHWKFNVDAAWSSPMPSTGIGVIGRKSDGSTGGISMKHLDGNFEGLGAELIAIK 195
           WSPP    +K N D AW       G+G++ R S G     +  H++  +    AE+ AI 
Sbjct: 26  WSPPRSGWFKINTDGAWDPNTSKGGVGLVTRDSFGLFLSAAAMHIN-TYSAEEAEIRAIW 85

Query: 196 VALELAVQMRCHKIIVESDSELAIKFINKS--LDVW-SEVECTVVAIWDLIPFFSEVVFN 255
            A++ A++ +   + +ESD+++ ++ + +      W ++     + +W L   F ++ FN
Sbjct: 86  TAMKQAMERKITALEIESDAKVVVEQLKRKNYCGAWNTDALLKDIEVWSL--SFEKLSFN 145

Query: 256 FIPRNVNRVADKLAKWAKMSKSDGIWVEDFSAWIVSMVEDDRFLC 298
           ++PR  NRVA +LA+W K S +D  W      W++ ++  D+FLC
Sbjct: 146 YVPRVCNRVAHELAQWGKNSTTDMFWGTP-PVWLLPVLRGDQFLC 186

BLAST of Spg022032 vs. NCBI nr
Match: XP_030487616.1 (uncharacterized protein LOC115704554 [Cannabis sativa])

HSP 1 Score: 84.0 bits (206), Expect = 2.5e-12
Identity = 52/155 (33.55%), Postives = 80/155 (51.61%), Query Frame = 0

Query: 136 WSPPPVDHWKFNVDAAWSSPMPSTGIGVIGRKSDGSTGGISMKHLDGNFEGLGAELIAIK 195
           W  PP    K NVDAA      + G+G + R SDG   G   K L G F    AE +AI 
Sbjct: 36  WVAPPACSLKLNVDAATDGENSTIGLGALVRDSDGFPLGCFAKSLTGFFSPKEAEALAIS 95

Query: 196 VALELAVQMRCHKIIVESDSELAIKFINKSLDVWSEVECTVVAIWDLIPFFSEVVFNFIP 255
            AL+  +++      VESD+E+ +  +N SL+  S+ +C V +I  L+  FS      + 
Sbjct: 96  YALKWFLELGFFVSFVESDAEIIVNSVNSSLECVSQWDCIVRSIRSLLFVFSGACLLHVM 155

Query: 256 RNVNRVADKLAKWAKMSKSDGIWVEDFSAWIVSMV 291
           RN N  A +LA++A   +++  W+E+    I++ V
Sbjct: 156 RNGNTAAYRLARYALQMENEVSWLEEIPPCIMTNV 190

BLAST of Spg022032 vs. NCBI nr
Match: KMS97072.1 (hypothetical protein BVRB_7g179330 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 80.5 bits (197), Expect = 2.8e-11
Identity = 54/196 (27.55%), Postives = 94/196 (47.96%), Query Frame = 0

Query: 98  SAGAAVDRCRLKYLEANSKNRDSEAVIRQSGQHALGRCWSPPPVDHWKFNVDAAWSSPMP 157
           SAG A +R  +  L  + K   S+   R+  + A  R W  P     K NVDAA +    
Sbjct: 619 SAGLAWERA-IDILRHHRKFNGSK---REGVKRAKDR-WLLPSPGFIKINVDAAVNGEYC 678

Query: 158 STGIGVIGRKSDGSTGGISMKHLDGNFEGLGAELIAIKVALELAVQMRCHKIIVESDSEL 217
             G+G++ R  +G       + +  ++    AE  A+  A E A+      +++ESD+++
Sbjct: 679 RVGLGMVARDENGQVLRAWSRTMSHDWPAEKAEAAAVLWATEEAIAQGWSNVVLESDAQV 738

Query: 218 AIKFINKSLDVWSEVECTVVAIWDLIPFFSEVVFNFIPRNVNRVADKLAKWAKMSKSDGI 277
            I  IN  L    +++  +  + +L+P F  + F+F  R  N +A +LAKWA  +  D +
Sbjct: 739 IINAINSRLQRRGDIQAMLEDVLNLVPSFETIKFSFCYRECNEIAHRLAKWATSNFCDEV 798

Query: 278 WVEDFSAWIVSMVEDD 294
           W+    +WI  ++  D
Sbjct: 799 WLVGGPSWIADLIVSD 809

BLAST of Spg022032 vs. ExPASy TrEMBL
Match: A0A803NYI1 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 1.2e-12
Identity = 52/155 (33.55%), Postives = 80/155 (51.61%), Query Frame = 0

Query: 136 WSPPPVDHWKFNVDAAWSSPMPSTGIGVIGRKSDGSTGGISMKHLDGNFEGLGAELIAIK 195
           W  PP    K NVDAA      + G+G + R SDG   G   K L G F    AE +AI 
Sbjct: 21  WVAPPACSLKLNVDAATDGENSTIGLGALVRDSDGFPLGCFAKSLTGFFSPKEAEALAIS 80

Query: 196 VALELAVQMRCHKIIVESDSELAIKFINKSLDVWSEVECTVVAIWDLIPFFSEVVFNFIP 255
            AL+  +++      VESD+E+ +  +N SL+  S+ +C V +I  L+  FS      + 
Sbjct: 81  YALKWFLELGFFVSFVESDAEIIVNSVNSSLECVSQWDCIVRSIRSLLFVFSGACLLHVM 140

Query: 256 RNVNRVADKLAKWAKMSKSDGIWVEDFSAWIVSMV 291
           RN N  A +LA++A   +++  W+E+    I++ V
Sbjct: 141 RNGNTAAYRLARYALQMENEVSWLEEIPPCIMTNV 175

BLAST of Spg022032 vs. ExPASy TrEMBL
Match: A0A0J8BAU9 (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris OX=3555 GN=BVRB_7g179330 PE=4 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 1.3e-11
Identity = 54/196 (27.55%), Postives = 94/196 (47.96%), Query Frame = 0

Query: 98  SAGAAVDRCRLKYLEANSKNRDSEAVIRQSGQHALGRCWSPPPVDHWKFNVDAAWSSPMP 157
           SAG A +R  +  L  + K   S+   R+  + A  R W  P     K NVDAA +    
Sbjct: 619 SAGLAWERA-IDILRHHRKFNGSK---REGVKRAKDR-WLLPSPGFIKINVDAAVNGEYC 678

Query: 158 STGIGVIGRKSDGSTGGISMKHLDGNFEGLGAELIAIKVALELAVQMRCHKIIVESDSEL 217
             G+G++ R  +G       + +  ++    AE  A+  A E A+      +++ESD+++
Sbjct: 679 RVGLGMVARDENGQVLRAWSRTMSHDWPAEKAEAAAVLWATEEAIAQGWSNVVLESDAQV 738

Query: 218 AIKFINKSLDVWSEVECTVVAIWDLIPFFSEVVFNFIPRNVNRVADKLAKWAKMSKSDGI 277
            I  IN  L    +++  +  + +L+P F  + F+F  R  N +A +LAKWA  +  D +
Sbjct: 739 IINAINSRLQRRGDIQAMLEDVLNLVPSFETIKFSFCYRECNEIAHRLAKWATSNFCDEV 798

Query: 278 WVEDFSAWIVSMVEDD 294
           W+    +WI  ++  D
Sbjct: 799 WLVGGPSWIADLIVSD 809

BLAST of Spg022032 vs. ExPASy TrEMBL
Match: A0A2I0B6G4 (RNase H domain-containing protein OS=Apostasia shenzhenica OX=1088818 GN=AXF42_Ash005277 PE=4 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 1.7e-11
Identity = 49/166 (29.52%), Postives = 83/166 (50.00%), Query Frame = 0

Query: 132 LGRCWSPPPVDHWKFNVDAAWSSPMPSTGIGVIGRKSDGSTGGISMKHLDGNFEGLGAEL 191
           L + W+PP     K NVDAA        G+GV+ R S G        ++ G+ E   AE 
Sbjct: 15  LQKVWTPPKHPTIKLNVDAAVDDKRGRLGVGVVARTSTGEVIACLSSNIHGSLEPTLAEC 74

Query: 192 IAIKVALELAVQMRCHKIIVESDSELAIKFINKSLDVWSEVECTVVAIWDLIPFFSEVVF 251
           +A++ A  LA +++   I +E DS  AIK I  + +  S +   + AI D +   S  + 
Sbjct: 75  LAVEQAFVLAEKIKASNIWIEGDSLEAIKLIQDTGEDLSIIHPHLKAINDRMKLLSHSII 134

Query: 252 NFIPRNVNRVADKLAKWAKMSKSDGIWVEDFSAWIVSMVEDDRFLC 298
             I R  NRVA  LA++A   ++  +W ++   +I +++ ++  +C
Sbjct: 135 THIRREGNRVAHTLARYALHIEAQKVWFKEAPFFIQAVIREEMLMC 180

BLAST of Spg022032 vs. ExPASy TrEMBL
Match: A0A200Q9T2 (Ribonuclease H domain OS=Macleaya cordata OX=56857 GN=BVC80_1609g31 PE=4 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 3.9e-11
Identity = 52/166 (31.33%), Postives = 85/166 (51.20%), Query Frame = 0

Query: 136 WSPPPVDHWKFNVDAAWSSPMPSTGIGVIGRKSDGSTGG---ISMKHLDGNFEGLGAELI 195
           W+PPP    K NVDA+WSS   + G+G+I R   GS  G   +++  LD       AE +
Sbjct: 277 WNPPPSSLIKINVDASWSSNSSTAGLGLIARDCAGSFRGARCLTVPALDAEH----AECL 336

Query: 196 AIKVALELAVQMRCHKIIVESDSELAIKFINKSLDV--WSEVECTVVAIWDLIPFFSEVV 255
           AI  A+  A ++  + + +E D++  + F+N       W        AI  L+  F   V
Sbjct: 337 AILHAIFWAQELSIYNVYIEGDNQAVLSFLNTGQGSIHWRNHNILDDAI-KLLSVFHFHV 396

Query: 256 FNFIPRNVNRVADKLAKWAKMSKSDGIWVEDFSAWIVSMVEDDRFL 297
           + ++ R+ NRVAD+LA +A+ + S   W  D   ++V ++  D+ L
Sbjct: 397 YKYVSRDCNRVADRLAAYARRNSSTKTWGLDVPPFLVDVLLQDQSL 437

BLAST of Spg022032 vs. ExPASy TrEMBL
Match: A0A7J6EJ00 (RNase H domain-containing protein OS=Cannabis sativa OX=3483 GN=G4B88_008543 PE=4 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 5.1e-11
Identity = 45/149 (30.20%), Postives = 76/149 (51.01%), Query Frame = 0

Query: 135 CWSPPPVDHWKFNVDAAWSSPMPSTGIGVIGRKSDGSTGGISMKHLDGNFEGLGAELIAI 194
           CWSPPP+  +  N DA+     P  G+G I R   G     + +++ G    L AE +AI
Sbjct: 111 CWSPPPLGSFMINTDASLIEGKPGCGLGFIIRDHLGELVTAATEYIPGCLSVLMAETLAI 170

Query: 195 KVALELAVQMRCHKIIVESDSE---LAIKFINKSLDVWSEV--ECTVVAIWDLIPFFSEV 254
           ++A++LA       I++ SD++    A+K   +    W ++  +CT+ +       F+ +
Sbjct: 171 RLAMKLAASRSLQNILIASDNQSVITALKGQTRFNTDWGKILEDCTIAS-----KHFNTL 230

Query: 255 VFNFIPRNVNRVADKLAKWAKMSKSDGIW 279
            FNF PR  N VA  LA W++++    +W
Sbjct: 231 SFNFTPRKCNNVAHCLANWSRVAHVSDVW 254

BLAST of Spg022032 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 72.0 bits (175), Expect = 9.1e-13
Identity = 45/132 (34.09%), Postives = 64/132 (48.48%), Query Frame = 0

Query: 136 WSPPPVDHWKFNVDAAWSSPMPSTGIGVIGRKSDGSTGGISMKHLDGNFEGLGAELIAIK 195
           W  PP    K N DA W    P  GIG I R   G    +  + L      L AEL A++
Sbjct: 137 WKAPPYQWVKCNTDATWQLENPRCGIGWILRNESGGVLWMGARALPRTKNVLEAELEALR 196

Query: 196 VALELAVQMRCHKIIVESDSELAIKFINKSLDVWSEVECTVVAIWDLIPFFSEVVFNFIP 255
            A+    +    +II ESD++  +  +N S D W  ++  +  I  L+  F EV F F P
Sbjct: 197 WAVLTMSRFNYKRIIFESDAQALVNLLN-SDDFWPTLQPALEDIQQLLHHFEEVKFEFTP 256

Query: 256 RNVNRVADKLAK 268
           R  N+VAD++A+
Sbjct: 257 RGGNKVADRIAR 267

BLAST of Spg022032 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 70.5 bits (171), Expect = 2.7e-12
Identity = 45/142 (31.69%), Postives = 75/142 (52.82%), Query Frame = 0

Query: 126 QSGQHALGRCWSPPPVDHWKFNVDAAWSSPMPSTGIGVIGRKSDGSTGGISMKHLDGNFE 185
           Q  + + GR W PPP    K N DA W+      GIG + R   G    +  + L     
Sbjct: 411 QVNRSSCGR-WRPPPHQWVKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARALPKLKS 470

Query: 186 GLGAELIAIKVALELAVQMRCHKIIVESDSELAIKFINKSLDVWSEVECTVVAIWDLIPF 245
            L AEL A++ A+    + + + +I ESDS++ I+ +N   ++W  ++ T+  +  L+  
Sbjct: 471 VLEAELEAMRWAVLSLSRFQYNYVIFESDSQVLIEILNND-EIWPSLKPTIQDLQRLLSQ 530

Query: 246 FSEVVFNFIPRNVNRVADKLAK 268
           F+EV F FIPR  N +A+++A+
Sbjct: 531 FTEVKFVFIPREGNTLAERVAR 550

BLAST of Spg022032 vs. TAIR 10
Match: AT5G65005.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 51.2 bits (121), Expect = 1.7e-06
Identity = 42/135 (31.11%), Postives = 59/135 (43.70%), Query Frame = 0

Query: 136 WSPPPVDHWKFNVDAAWSSPMPSTGIGVIGRKSDGSTGGISMKHLDGNFEGLGAELIAIK 195
           WSPP  D  K N DA+       +G+G I R S G+     M    G      AE   + 
Sbjct: 105 WSPPGRDKLKCNYDASHHERNTVSGLGWILRNSQGTVIECGMGKFQGRMTTEEAECSTLI 164

Query: 196 VALELAVQMRCHKIIVESDSELAIKFIN-KSLDVWSEVECTVVAIWDLIPFFSEVVFNFI 255
            A++ +      K+I E D++   + IN KS +   +     +  W  IP F  + F+F 
Sbjct: 165 WAIQASYGFGHKKVIFEGDNQTITRMINTKSSNPRLQHFLDTIQSW--IPSFESIEFSFK 224

Query: 256 PRNVNRVADKLAKWA 270
            R  N  AD LAK A
Sbjct: 225 HREQNGCADFLAKQA 237

BLAST of Spg022032 vs. TAIR 10
Match: AT4G09490.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 50.4 bits (119), Expect = 2.8e-06
Identity = 44/136 (32.35%), Postives = 64/136 (47.06%), Query Frame = 0

Query: 139 PPVDHWKFNVDAAWSSPMPSTGIGVIGRKSDGSTGGISMKHLDGNFEGLGAELIAIKVAL 198
           P +D      DAAW       G G + R     T              L AE IA+ +AL
Sbjct: 26  PDLDRVTIFTDAAWKHETKDVGFGWVIRNCPELTALHYQSAARNVRLPLMAEAIALFLAL 85

Query: 199 ELAVQMRCHKIIVESDSELAIKFINKSLDVWSEVECTVVAIWDLIPFFSEVVFNFIPRNV 258
           + A  +   K+ + SDS+  I  I  S    +E    +  I +L   F++V F+F+PR+ 
Sbjct: 86  QYAQSIGITKLSMASDSQQLITAIT-SESPSTEFYGIIFDILNLSLGFADVSFSFVPRSE 145

Query: 259 NRVADKLAKWAKMSKS 275
           NRVAD+LAK + +S S
Sbjct: 146 NRVADELAKSSLISFS 160

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038902513.12.3e-1334.00uncharacterized protein LOC120089172 [Benincasa hispida][more]
OVA10548.11.9e-1229.09Ribonuclease H domain [Macleaya cordata][more]
OVA19644.11.9e-1229.09Ribonuclease H domain [Macleaya cordata][more]
XP_030487616.12.5e-1233.55uncharacterized protein LOC115704554 [Cannabis sativa][more]
KMS97072.12.8e-1127.55hypothetical protein BVRB_7g179330 [Beta vulgaris subsp. vulgaris][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A803NYI11.2e-1233.55Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A0J8BAU91.3e-1127.55Uncharacterized protein OS=Beta vulgaris subsp. vulgaris OX=3555 GN=BVRB_7g17933... [more]
A0A2I0B6G41.7e-1129.52RNase H domain-containing protein OS=Apostasia shenzhenica OX=1088818 GN=AXF42_A... [more]
A0A200Q9T23.9e-1131.33Ribonuclease H domain OS=Macleaya cordata OX=56857 GN=BVC80_1609g31 PE=4 SV=1[more]
A0A7J6EJ005.1e-1130.20RNase H domain-containing protein OS=Cannabis sativa OX=3483 GN=G4B88_008543 PE=... [more]
Match NameE-valueIdentityDescription
AT2G34320.19.1e-1334.09Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT4G29090.12.7e-1231.69Ribonuclease H-like superfamily protein [more]
AT5G65005.11.7e-0631.11Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT4G09490.12.8e-0632.35Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 147..269
e-value: 1.3E-21
score: 76.7
IPR002156Ribonuclease H domainPROSITEPS50879RNASE_Hcoord: 172..271
score: 9.448068
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 142..270
e-value: 5.3E-17
score: 64.1
NoneNo IPR availablePANTHERPTHR33033POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 134..293
NoneNo IPR availablePANTHERPTHR33033:SF67SUBFAMILY NOT NAMEDcoord: 134..293
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 147..267
e-value: 8.0976E-23
score: 89.2956
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 142..270

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg022032.1Spg022032.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity