Homology
BLAST of Spg021976 vs. NCBI nr
Match:
XP_022933611.1 (flavonoid 3'-monooxygenase [Cucurbita moschata])
HSP 1 Score: 900.6 bits (2326), Expect = 1.1e-257
Identity = 451/550 (82.00%), Postives = 483/550 (87.82%), Query Frame = 0
Query: 1 MDEISLIVAAAAVTLASAVLLHRFLFLRRSSQSLRLPPGPKPWPIVGNLPQIGGAPLHSH 60
MDEISL +AA+TLAS VLL+R LF +R+S+SL LPPGPKPWPIVGNLPQIGGAPLHS
Sbjct: 1 MDEISLFF-SAAITLASVVLLYRLLFHQRNSRSLPLPPGPKPWPIVGNLPQIGGAPLHSL 60
Query: 61 LAAMAGEYGPLMYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDM 120
LA M+GEYGPLMYLRFGSVDVVVAASAA+AEQFLKIRD NFLSRPPNAGAKYIAYNYQDM
Sbjct: 61 LADMSGEYGPLMYLRFGSVDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDM 120
Query: 121 VFAPYGPRWRLLRKISAVHLFSSKALDDHRRVREEEVAVLVQTLASAGKAPATLGSLLTI 180
VFAPYGPRWRLLRKISAVHLFSSKAL+DHRRVREEEV VLVQ LA +G+APATLGSLLT+
Sbjct: 121 VFAPYGPRWRLLRKISAVHLFSSKALEDHRRVREEEVGVLVQALARSGEAPATLGSLLTV 180
Query: 181 CTANALGRTMIGRRVFGDGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLDLLG 240
CTANALGRTMIGRRVFGDGS GGDP DQFKA+VEQVM+LSGKFNPGDFF LEWLDLLG
Sbjct: 181 CTANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGDFFSWLEWLDLLG 240
Query: 241 VAGEMKKVHKWFDDFLTKILDEHRNLLAGAGGSQHQDLLSMLLSSKDQAEDENEKLSDTE 300
VAGEMKKVHKWFDD ++KIL+EHRNLLA GG +HQDLLS LLS++D+A+DENEKL+DTE
Sbjct: 241 VAGEMKKVHKWFDDLMSKILEEHRNLLATGGGGEHQDLLSTLLSTRDRAKDENEKLNDTE 300
Query: 301 IKALLLVLFLEQNLAAYWYYDFPTYPIKILATKRCGENNMFTAGTDTSASTVEWALAELI 360
IKALLL NMFTAGTDTSASTVEWALAELI
Sbjct: 301 IKALLL--------------------------------NMFTAGTDTSASTVEWALAELI 360
Query: 361 RHPKMLAQTQQELDSVVGRDRLVSDLDLPRLGYLQAVVKETFRLHPPTPLSLPRMAAESC 420
RHPKM+ Q QQELDSVVGRDR+VSDLDLP+LGYLQAVVKETFRLHPPTPLSLPRMAAESC
Sbjct: 361 RHPKMMYQAQQELDSVVGRDRVVSDLDLPKLGYLQAVVKETFRLHPPTPLSLPRMAAESC 420
Query: 421 EINGFHIPKGSTLLVDVWAIGRDPKEWADPLEFRPNRFLPGGEKAHVDVKGNDFEVIPFG 480
EI+G+HIPKGSTLLVDVWAI RDPKEW PLEFRP+RFLPGGEKAHVDVKGNDF VIPFG
Sbjct: 421 EISGYHIPKGSTLLVDVWAISRDPKEWTSPLEFRPDRFLPGGEKAHVDVKGNDFGVIPFG 480
Query: 481 AGRRICVGMSLGLRTVQMLTATLVHSFDWALPIGLSPDKLNMDEAFGLTLRRAQPLALHP 540
AGRRICVGMS+GLR VQMLTA+LVHSFDWALP GLSP +LNMDEAFGLTLRRAQPL HP
Sbjct: 481 AGRRICVGMSMGLRMVQMLTASLVHSFDWALPSGLSPGELNMDEAFGLTLRRAQPLVAHP 517
Query: 541 RPRLSPEVYQ 551
RPRLSP VYQ
Sbjct: 541 RPRLSPGVYQ 517
BLAST of Spg021976 vs. NCBI nr
Match:
KAG6587576.1 (Flavonoid 3'-monooxygenase, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 898.3 bits (2320), Expect = 5.3e-257
Identity = 450/550 (81.82%), Postives = 481/550 (87.45%), Query Frame = 0
Query: 1 MDEISLIVAAAAVTLASAVLLHRFLFLRRSSQSLRLPPGPKPWPIVGNLPQIGGAPLHSH 60
MDEISL +AA+TLAS VLL+R LF +R+S+SL LPPGPKPWPIVGNLPQIGGAPLHS
Sbjct: 92 MDEISLFF-SAAITLASVVLLYRLLFHQRNSRSLPLPPGPKPWPIVGNLPQIGGAPLHSL 151
Query: 61 LAAMAGEYGPLMYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDM 120
LA M+GEYGPLMYLRFGSVDVVVAASAA+AEQFLKIRD NFLSRPPNAGAKYIAYNYQDM
Sbjct: 152 LADMSGEYGPLMYLRFGSVDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDM 211
Query: 121 VFAPYGPRWRLLRKISAVHLFSSKALDDHRRVREEEVAVLVQTLASAGKAPATLGSLLTI 180
VFAPYGPRWRLLRKISAVHLFSSKAL+DHRRVREEEV VLVQ LA G+APATLGSLLT+
Sbjct: 212 VFAPYGPRWRLLRKISAVHLFSSKALEDHRRVREEEVGVLVQALARPGEAPATLGSLLTV 271
Query: 181 CTANALGRTMIGRRVFGDGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLDLLG 240
CTANALGRTMIGRRVFGDGS GGDP DQFKA+VEQVM+LSGKFNPGDFFP LEWLDLLG
Sbjct: 272 CTANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGDFFPWLEWLDLLG 331
Query: 241 VAGEMKKVHKWFDDFLTKILDEHRNLLAGAGGSQHQDLLSMLLSSKDQAEDENEKLSDTE 300
VA EMKKVHKWFDD ++KIL+EHRNLLA GG +HQDLL+ LLS++D A+DENEKL+DTE
Sbjct: 332 VAREMKKVHKWFDDLMSKILEEHRNLLATGGGGEHQDLLTTLLSTRDHAKDENEKLNDTE 391
Query: 301 IKALLLVLFLEQNLAAYWYYDFPTYPIKILATKRCGENNMFTAGTDTSASTVEWALAELI 360
IKALLL NMFTAGTDTSASTVEWALAELI
Sbjct: 392 IKALLL--------------------------------NMFTAGTDTSASTVEWALAELI 451
Query: 361 RHPKMLAQTQQELDSVVGRDRLVSDLDLPRLGYLQAVVKETFRLHPPTPLSLPRMAAESC 420
RHPKM+ Q QQELDSVVGRDR+VSDLDLP+LGYLQAVVKETFRLHPPTPLSLPRMAAESC
Sbjct: 452 RHPKMMYQAQQELDSVVGRDRVVSDLDLPKLGYLQAVVKETFRLHPPTPLSLPRMAAESC 511
Query: 421 EINGFHIPKGSTLLVDVWAIGRDPKEWADPLEFRPNRFLPGGEKAHVDVKGNDFEVIPFG 480
EI+G+HIPKGSTLLVDVWAI RDPKEW PLEFRP+RFLPGGEKAHVDVKGNDF VIPFG
Sbjct: 512 EISGYHIPKGSTLLVDVWAISRDPKEWTSPLEFRPDRFLPGGEKAHVDVKGNDFGVIPFG 571
Query: 481 AGRRICVGMSLGLRTVQMLTATLVHSFDWALPIGLSPDKLNMDEAFGLTLRRAQPLALHP 540
AGRRICVGMS+GLR VQMLTA+LVHSFDWALP GLSP +LNMDEAFGLTLRRAQPL HP
Sbjct: 572 AGRRICVGMSMGLRMVQMLTASLVHSFDWALPSGLSPGELNMDEAFGLTLRRAQPLVAHP 608
Query: 541 RPRLSPEVYQ 551
RPRLSP VYQ
Sbjct: 632 RPRLSPGVYQ 608
BLAST of Spg021976 vs. NCBI nr
Match:
XP_023529494.1 (flavonoid 3'-monooxygenase [Cucurbita pepo subsp. pepo] >XP_023529495.1 flavonoid 3'-monooxygenase [Cucurbita pepo subsp. pepo])
HSP 1 Score: 897.1 bits (2317), Expect = 1.2e-256
Identity = 451/553 (81.56%), Postives = 484/553 (87.52%), Query Frame = 0
Query: 1 MDEISLIVAAAAVTLASAVLLHRFLFLRRSSQSLRLPPGPKPWPIVGNLPQIGGAPLHSH 60
MDEISL +AA+TLAS VLL+R LF +R+S+SL LPPGPKPWPIVGNLPQIGGAPLHS
Sbjct: 1 MDEISLFF-SAAITLASVVLLYRLLFHQRNSRSLPLPPGPKPWPIVGNLPQIGGAPLHSL 60
Query: 61 LAAMAGEYGPLMYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDM 120
LA M+GEYGPLMYLRFGSVDVVVAASAA+AEQFLKIRD NFLSRPPNAGAKYIAYNYQDM
Sbjct: 61 LADMSGEYGPLMYLRFGSVDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDM 120
Query: 121 VFAPYGPRWRLLRKISAVHLFSSKALDDHRRVREEEVAVLVQTLASAGKAPATLGSLLTI 180
VFAPYGPRWRLLRKISAVHLFSSKAL+DHRRVREEEV VLVQ LA +G+APATLGSLLT+
Sbjct: 121 VFAPYGPRWRLLRKISAVHLFSSKALEDHRRVREEEVGVLVQALARSGEAPATLGSLLTV 180
Query: 181 CTANALGRTMIGRRVFGDGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLDLLG 240
CTANALGRTMIGRRVFGDGS GGDP DQFKA+VEQVM+LSGKFNPGDFFPSL+WLDLLG
Sbjct: 181 CTANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGDFFPSLDWLDLLG 240
Query: 241 VAGEMKKVHKWFDDFLTKILDEHRNLLA---GAGGSQHQDLLSMLLSSKDQAEDENEKLS 300
VA EMKKVHKWFDD L+KIL+EHRNLLA G GG +HQDLLS LLS++D+AEDENEKL+
Sbjct: 241 VAREMKKVHKWFDDLLSKILEEHRNLLASGGGGGGGEHQDLLSTLLSARDRAEDENEKLN 300
Query: 301 DTEIKALLLVLFLEQNLAAYWYYDFPTYPIKILATKRCGENNMFTAGTDTSASTVEWALA 360
DTEIKALLL NMFTAGTDTSASTVEWALA
Sbjct: 301 DTEIKALLL--------------------------------NMFTAGTDTSASTVEWALA 360
Query: 361 ELIRHPKMLAQTQQELDSVVGRDRLVSDLDLPRLGYLQAVVKETFRLHPPTPLSLPRMAA 420
ELIRHPKM+ Q QQELDS+VGRDR+VSDLDLP+LGYLQAVVKETFRLHPPTPLSLPRMAA
Sbjct: 361 ELIRHPKMMHQAQQELDSIVGRDRVVSDLDLPKLGYLQAVVKETFRLHPPTPLSLPRMAA 420
Query: 421 ESCEINGFHIPKGSTLLVDVWAIGRDPKEWADPLEFRPNRFLPGGEKAHVDVKGNDFEVI 480
ESCEI+G+ IPKGSTLLVDVWAI RDPKEW PLEFRP+RFLPGGEKAHVDVKGNDF VI
Sbjct: 421 ESCEISGYRIPKGSTLLVDVWAISRDPKEWTSPLEFRPDRFLPGGEKAHVDVKGNDFGVI 480
Query: 481 PFGAGRRICVGMSLGLRTVQMLTATLVHSFDWALPIGLSPDKLNMDEAFGLTLRRAQPLA 540
PFGAGRRICVGMS+GLR VQ+LTA+LVHSFDWALP GLSP +LNMDEAFGLTLRRAQPL
Sbjct: 481 PFGAGRRICVGMSMGLRMVQILTASLVHSFDWALPSGLSPGELNMDEAFGLTLRRAQPLV 520
Query: 541 LHPRPRLSPEVYQ 551
HPRPRLSP VYQ
Sbjct: 541 AHPRPRLSPGVYQ 520
BLAST of Spg021976 vs. NCBI nr
Match:
XP_023007386.1 (flavonoid 3'-monooxygenase [Cucurbita maxima])
HSP 1 Score: 888.6 bits (2295), Expect = 4.2e-254
Identity = 448/550 (81.45%), Postives = 479/550 (87.09%), Query Frame = 0
Query: 1 MDEISLIVAAAAVTLASAVLLHRFLFLRRSSQSLRLPPGPKPWPIVGNLPQIGGAPLHSH 60
MDEISL +AA+TLA+ VLL+R LF +R+S+SL LPPGPKPWP VGNLPQIGGAPLHS
Sbjct: 1 MDEISLFF-SAAITLATVVLLYRLLFHQRNSRSLPLPPGPKPWPTVGNLPQIGGAPLHSQ 60
Query: 61 LAAMAGEYGPLMYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDM 120
LAAM+GEYGPLMYLRFGS+DVVVAASAA+AEQFLKIRD NFLSRPPNAGAKYIAYNYQDM
Sbjct: 61 LAAMSGEYGPLMYLRFGSIDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDM 120
Query: 121 VFAPYGPRWRLLRKISAVHLFSSKALDDHRRVREEEVAVLVQTLASAGKAPATLGSLLTI 180
VFAPYGPRWRLLRKISAVHLFSSKAL+D+R VREEEV VLVQ LA G+APATLGSLLT+
Sbjct: 121 VFAPYGPRWRLLRKISAVHLFSSKALEDNRCVREEEVGVLVQALARPGEAPATLGSLLTV 180
Query: 181 CTANALGRTMIGRRVFGDGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLDLLG 240
CTANALGRTMIGRRVFGDGS GGDP DQFKA+VEQVM+LSGKFNPG+FFPSLEWLDLLG
Sbjct: 181 CTANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGNFFPSLEWLDLLG 240
Query: 241 VAGEMKKVHKWFDDFLTKILDEHRNLLAGAGGSQHQDLLSMLLSSKDQAEDENEKLSDTE 300
VA EMKKVHKWFDD L+KIL+EHR LL A G +HQDLLS LLS++D AEDENEKL+DTE
Sbjct: 241 VAREMKKVHKWFDDLLSKILEEHRTLL--ASGGEHQDLLSTLLSTRDHAEDENEKLNDTE 300
Query: 301 IKALLLVLFLEQNLAAYWYYDFPTYPIKILATKRCGENNMFTAGTDTSASTVEWALAELI 360
IKALLL NMFTAGTDTSASTVEWALAELI
Sbjct: 301 IKALLL--------------------------------NMFTAGTDTSASTVEWALAELI 360
Query: 361 RHPKMLAQTQQELDSVVGRDRLVSDLDLPRLGYLQAVVKETFRLHPPTPLSLPRMAAESC 420
RHPKM+ Q QQELDSVVGRDR+VSDLDLP+LGYLQAVVKETFRLHPPTPLSLPRMAAESC
Sbjct: 361 RHPKMMYQAQQELDSVVGRDRVVSDLDLPKLGYLQAVVKETFRLHPPTPLSLPRMAAESC 420
Query: 421 EINGFHIPKGSTLLVDVWAIGRDPKEWADPLEFRPNRFLPGGEKAHVDVKGNDFEVIPFG 480
EING+HIPKGSTLLVDVWAI RDPKEW PLEFRP+RFLPGGEKAHVDVKGNDF VIPFG
Sbjct: 421 EINGYHIPKGSTLLVDVWAISRDPKEWTCPLEFRPDRFLPGGEKAHVDVKGNDFGVIPFG 480
Query: 481 AGRRICVGMSLGLRTVQMLTATLVHSFDWALPIGLSPDKLNMDEAFGLTLRRAQPLALHP 540
AGRRICVGMS+GLR VQMLTA+LVHSFDWALP GLSP +LNMDEAFGLTLRRAQPL HP
Sbjct: 481 AGRRICVGMSMGLRMVQMLTASLVHSFDWALPSGLSPGELNMDEAFGLTLRRAQPLVAHP 515
Query: 541 RPRLSPEVYQ 551
RPRLSP VYQ
Sbjct: 541 RPRLSPGVYQ 515
BLAST of Spg021976 vs. NCBI nr
Match:
XP_038878698.1 (flavonoid 3'-monooxygenase [Benincasa hispida])
HSP 1 Score: 874.0 bits (2257), Expect = 1.1e-249
Identity = 445/553 (80.47%), Postives = 471/553 (85.17%), Query Frame = 0
Query: 1 MDEISLIVAAAAVTLASAVLLHRFLFLRRSSQSLRLPPGPKPWPIVGNLPQIGGAPLHSH 60
MDEI+LIV AA VTLAS +LLHR F RRS RLPPGPKPWPIVGN+PQIGGAPLHS
Sbjct: 1 MDEIALIV-AAVVTLASTILLHR-TFFRRS--RFRLPPGPKPWPIVGNMPQIGGAPLHSL 60
Query: 61 LAAMAGEYGPLMYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDM 120
LAAMAGEYG LMYLR GSV VVVAASAAVAE+ LK+ D NFLSRPPNAGAKYIAYNYQDM
Sbjct: 61 LAAMAGEYGSLMYLRLGSVGVVVAASAAVAEKLLKVHDTNFLSRPPNAGAKYIAYNYQDM 120
Query: 121 VFAPYGPRWRLLRKISAVHLFSSKALDDHRRVREEEVAVLVQTLASAGKAPATLGSLLTI 180
VFAPYGPRWRLLRKIS VHLFSSKALDDHRR+REEE+AVLVQ LAS+G+ PA LGSLLT+
Sbjct: 121 VFAPYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIAVLVQALASSGEVPAALGSLLTV 180
Query: 181 CTANALGRTMIGRRVFGDGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLDLLG 240
CTANALGRTMIGRRVFGDG G D + QFKA+VEQVM+L+GKFNPGDFFPSLEWLDLLG
Sbjct: 181 CTANALGRTMIGRRVFGDGGSGDDSEAKQFKAMVEQVMILAGKFNPGDFFPSLEWLDLLG 240
Query: 241 VAGEMKKVHKWFDDFLTKILDEHRNLLAGAGGSQHQDLLSMLLSSKDQAEDENEKLSDTE 300
VA EMKKVHKWFDDFLTKI++EHRNLLA G HQDLLS LLSS AEDENEK++DTE
Sbjct: 241 VASEMKKVHKWFDDFLTKIVEEHRNLLARGRGGGHQDLLSTLLSS---AEDENEKINDTE 300
Query: 301 IKALLLVLFLEQNLAAYWYYDFPTYPIKILATKRCGENNMFTAGTDTSASTVEWALAELI 360
IKALLL NMFTAGTDTSASTVEWAL ELI
Sbjct: 301 IKALLL--------------------------------NMFTAGTDTSASTVEWALTELI 360
Query: 361 RHPKMLAQTQQELDSVVGRDRLVSDLDLPRLGYLQAVVKETFRLHPPTPLSLPRMAAESC 420
RHPKM+AQ QQELDSVVGRDR VSDLDLP+L YLQAVVKETFRLHPPTPLSLPRMAAESC
Sbjct: 361 RHPKMMAQAQQELDSVVGRDRAVSDLDLPQLVYLQAVVKETFRLHPPTPLSLPRMAAESC 420
Query: 421 EINGFHIPKGSTLLVDVWAIGRDPKEWADPLEFRPNRFLPGGEKAHVDVKGNDFEVIPFG 480
EING+HIPKG+TLLVDVWAIGRDPKEWADPLEFRPNRFLPGGEKAHVDVKGNDFEVIPFG
Sbjct: 421 EINGYHIPKGTTLLVDVWAIGRDPKEWADPLEFRPNRFLPGGEKAHVDVKGNDFEVIPFG 480
Query: 481 AGRRICVGMSLGLRTVQMLTATLVHSFDWALPIGLSPDKLNMDEAFGLTLRRAQPLALHP 540
AGRRICVGMS+GLR VQMLTATLVH FDWALP GL PD+LNMDEA+GLTLRRAQPL +HP
Sbjct: 481 AGRRICVGMSMGLRMVQMLTATLVHYFDWALPNGLLPDELNMDEAYGLTLRRAQPLVVHP 514
Query: 541 RPRLSPEVYQGLP 554
RPRL P VYQ P
Sbjct: 541 RPRLLPGVYQKSP 514
BLAST of Spg021976 vs. ExPASy Swiss-Prot
Match:
Q9SBQ9 (Flavonoid 3'-monooxygenase OS=Petunia hybrida OX=4102 GN=CYP75B2 PE=2 SV=1)
HSP 1 Score: 656.0 bits (1691), Expect = 6.0e-187
Identity = 331/552 (59.96%), Postives = 405/552 (73.37%), Query Frame = 0
Query: 1 MDEISLIVAAAAVTLASAVLLHRFLFLRRSSQSLRLPPGPKPWPIVGNLPQIGGAPLHSH 60
M+ +SLI+ + +L F R L LPPGPKPWPI+GNL +G P H
Sbjct: 1 MEILSLILYTVIFSFLLQFILRSFF---RKRYPLPLPPGPKPWPIIGNLVHLGPKP-HQS 60
Query: 61 LAAMAGEYGPLMYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDM 120
AAMA YGPLMYL+ G VDVVVAASA+VA QFLK D NF SRPPN+GA+++AYNYQD+
Sbjct: 61 TAAMAQTYGPLMYLKMGFVDVVVAASASVAAQFLKTHDANFSSRPPNSGAEHMAYNYQDL 120
Query: 121 VFAPYGPRWRLLRKISAVHLFSSKALDDHRRVREEEVAVLVQTLASAGKAPATLGSLLTI 180
VFAPYGPRWR+LRKI +VHLFS+KALDD R VR++EV L + LASAG+ P LG LL +
Sbjct: 121 VFAPYGPRWRMLRKICSVHLFSTKALDDFRHVRQDEVKTLTRALASAGQKPVKLGQLLNV 180
Query: 181 CTANALGRTMIGRRVFGDGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLDLLG 240
CT NAL R M+G+RVF DGS DP+ +FK++V ++MV++G FN GDF P L WLD+ G
Sbjct: 181 CTTNALARVMLGKRVFADGSGDVDPQAAEFKSMVVEMMVVAGVFNIGDFIPQLNWLDIQG 240
Query: 241 VAGEMKKVHKWFDDFLTKILDEHRNLLAGAGGSQHQDLLSMLLSSK-DQAEDENEKLSDT 300
VA +MKK+H FD FLT IL+EH+ + G + +DLLS L+S K D A+++ KL+DT
Sbjct: 241 VAAKMKKLHARFDAFLTDILEEHKGKIFG----EMKDLLSTLISLKNDDADNDGGKLTDT 300
Query: 301 EIKALLLVLFLEQNLAAYWYYDFPTYPIKILATKRCGENNMFTAGTDTSASTVEWALAEL 360
EIKALLL N+F AGTDTS+STVEWA+AEL
Sbjct: 301 EIKALLL--------------------------------NLFVAGTDTSSSTVEWAIAEL 360
Query: 361 IRHPKMLAQTQQELDSVVGRDRLVSDLDLPRLGYLQAVVKETFRLHPPTPLSLPRMAAES 420
IR+PK+LAQ QQE+D VVGRDRLV +LDL +L YL+A+VKETFRLHP TPLSLPR+A+ES
Sbjct: 361 IRNPKILAQAQQEIDKVVGRDRLVGELDLAQLTYLEAIVKETFRLHPSTPLSLPRIASES 420
Query: 421 CEINGFHIPKGSTLLVDVWAIGRDPKEWADPLEFRPNRFLPGGEKAHVDVKGNDFEVIPF 480
CEING+ IPKGSTLL++VWAI RDP WADPLEFRP RFLPGGEK VDV+GNDFEVIPF
Sbjct: 421 CEINGYFIPKGSTLLLNVWAIARDPNAWADPLEFRPERFLPGGEKPKVDVRGNDFEVIPF 480
Query: 481 GAGRRICVGMSLGLRTVQMLTATLVHSFDWALPIGLSPDKLNMDEAFGLTLRRAQPLALH 540
GAGRRIC GM+LG+R VQ++ ATL+H+F+W L G P+ LNM+EA+GLTL+RA PL +H
Sbjct: 481 GAGRRICAGMNLGIRMVQLMIATLIHAFNWDLVSGQLPEMLNMEEAYGLTLQRADPLVVH 512
Query: 541 PRPRLSPEVYQG 552
PRPRL + Y G
Sbjct: 541 PRPRLEAQAYIG 512
BLAST of Spg021976 vs. ExPASy Swiss-Prot
Match:
Q9SD85 (Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP75B1 PE=1 SV=1)
HSP 1 Score: 639.4 bits (1648), Expect = 5.8e-182
Identity = 321/545 (58.90%), Postives = 396/545 (72.66%), Query Frame = 0
Query: 14 TLASAVLLHRFLFL--------RRSSQSLRLPPGPKPWPIVGNLPQIGGAPLHSHLAAMA 73
TL +LL LFL R S + RLPPGP PWPI+GNLP +G P H L+AM
Sbjct: 3 TLFLTILLATVLFLILRIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMGTKP-HRTLSAMV 62
Query: 74 GEYGPLMYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDMVFAPY 133
YGP+++LR G VDVVVAAS +VAEQFLKI D NF SRPPN+GAK++AYNYQD+VFAPY
Sbjct: 63 TTYGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAPY 122
Query: 134 GPRWRLLRKISAVHLFSSKALDDHRRVREEEVAVLVQTLASAGKAPATLGSLLTICTANA 193
G RWRLLRKIS+VHLFS+KAL+D + VR+EEV L + L G P LG L+ +C NA
Sbjct: 123 GHRWRLLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKPVNLGQLVNMCVVNA 182
Query: 194 LGRTMIGRRVFGDGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLDLLGVAGEM 253
LGR MIGRR+FG + D K D+F+++V ++M L+G FN GDF PSL+WLDL GVAG+M
Sbjct: 183 LGREMIGRRLFGADA---DHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGKM 242
Query: 254 KKVHKWFDDFLTKILDEHRNLLAGAGGSQHQDLLSMLLSSK-DQAEDENEKLSDTEIKAL 313
K++HK FD FL+ IL EH +H D+LS L+S K + + L+DTEIKAL
Sbjct: 243 KRLHKRFDAFLSSILKEHE---MNGQDQKHTDMLSTLISLKGTDLDGDGGSLTDTEIKAL 302
Query: 314 LLVLFLEQNLAAYWYYDFPTYPIKILATKRCGENNMFTAGTDTSASTVEWALAELIRHPK 373
LL NMFTAGTDTSASTV+WA+AELIRHP
Sbjct: 303 LL--------------------------------NMFTAGTDTSASTVDWAIAELIRHPD 362
Query: 374 MLAQTQQELDSVVGRDRLVSDLDLPRLGYLQAVVKETFRLHPPTPLSLPRMAAESCEING 433
++ + Q+ELD VVGRDR V++ D+ +L YLQAV+KE FRLHPPTPLSLP +A+ESCEING
Sbjct: 363 IMVKAQEELDIVVGRDRPVNESDIAQLPYLQAVIKENFRLHPPTPLSLPHIASESCEING 422
Query: 434 FHIPKGSTLLVDVWAIGRDPKEWADPLEFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRR 493
+HIPKGSTLL ++WAI RDP +W+DPL F+P RFLPGGEK+ VDVKG+DFE+IPFGAGRR
Sbjct: 423 YHIPKGSTLLTNIWAIARDPDQWSDPLAFKPERFLPGGEKSGVDVKGSDFELIPFGAGRR 482
Query: 494 ICVGMSLGLRTVQMLTATLVHSFDWALPIGLSPDKLNMDEAFGLTLRRAQPLALHPRPRL 550
IC G+SLGLRT+Q LTATLV FDW L G++P+KLNM+E++GLTL+RA PL +HP+PRL
Sbjct: 483 ICAGLSLGLRTIQFLTATLVQGFDWELAGGVTPEKLNMEESYGLTLQRAVPLVVHPKPRL 508
BLAST of Spg021976 vs. ExPASy Swiss-Prot
Match:
A0A4D6Q415 (Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN=CYP75B137 PE=1 SV=1)
HSP 1 Score: 597.0 bits (1538), Expect = 3.3e-169
Identity = 307/539 (56.96%), Postives = 386/539 (71.61%), Query Frame = 0
Query: 14 TLASAVLLHRFLFLRRSSQSLRLPPGPKPWPIVGNLPQIGGAPLHSHLAAMAGEYGPLMY 73
TL + + L+ RRS+Q LPPGP WPI+GNLPQ+G P H L A++ +YGPL
Sbjct: 11 TLLLSSFIVYLLYRRRSAQCPPLPPGPNGWPILGNLPQLGAKP-HQTLDALSKQYGPLFR 70
Query: 74 LRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDMVFAPYGPRWRLLR 133
LR GSV+VVVA+S+AVA QFL+ DVNF +RPPN+GA+++AYNYQD+VFAPYGPRWR+LR
Sbjct: 71 LRLGSVNVVVASSSAVAAQFLRTHDVNFSNRPPNSGAEHVAYNYQDLVFAPYGPRWRMLR 130
Query: 134 KISAVHLFSSKALDDHRRVREEEVAVLVQTL---ASAGKAPATLGSLLTICTANALGRTM 193
K+ +VHLFS KALDD R VR+ EVA LV+ L A G LG L +C NAL R M
Sbjct: 131 KLCSVHLFSLKALDDLRPVRQGEVACLVRNLRRHADTG-VLVNLGKALNVCATNALARAM 190
Query: 194 IGRRVFGDGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLDLLGVAGEMKKVHK 253
+GRRVF D D + D+FK +V ++M L+G FN GDF P L WLDL GV G+MK++H+
Sbjct: 191 LGRRVFAD-EDAQLAEADEFKEMVVELMRLAGVFNVGDFVPGLGWLDLQGVVGKMKRLHR 250
Query: 254 WFDDFLTKILDEHRNLLAGAGGSQHQDLLSMLLSSKDQAEDENEKLSDTEIKALLLVLFL 313
+D FL ++++E++ ++ DLLS+L+ K+ + KL++T+IKALLL
Sbjct: 251 RYDAFLDRVIEENQ------ANAKSGDLLSVLIRLKEADAEGEIKLNNTDIKALLL---- 310
Query: 314 EQNLAAYWYYDFPTYPIKILATKRCGENNMFTAGTDTSASTVEWALAELIRHPKMLAQTQ 373
N+FTAGTDTS+STVEW LAELIRHP +L +TQ
Sbjct: 311 ----------------------------NLFTAGTDTSSSTVEWVLAELIRHPDILQKTQ 370
Query: 374 QELDSVVGRDRLVSDLDLPRLGYLQAVVKETFRLHPPTPLSLPRMAAESCEINGFHIPKG 433
ELDSV+GRDRLV++ DLP L YLQAVVKETFRLHP TPLSLPRMA+E C +NG+ IPK
Sbjct: 371 HELDSVIGRDRLVAESDLPNLPYLQAVVKETFRLHPSTPLSLPRMASEECIVNGYKIPKH 430
Query: 434 STLLVDVWAIGRDPKEWADPLEFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMS 493
+TLLV+VW+IGRD W DPLEFRP+RFLPGGE+ HVDVKGNDFEVIPFGAGRRIC G+S
Sbjct: 431 ATLLVNVWSIGRDAAVWNDPLEFRPSRFLPGGEREHVDVKGNDFEVIPFGAGRRICAGLS 490
Query: 494 LGLRTVQMLTATLVHSFDWALPIGLSPDKLNMDEAFGLTLRRAQPLALHPRPRLSPEVY 550
LGLR VQ +TAT+VH++DW+LP G KL+M+EA+GLTL+RA PL + P PRLS + Y
Sbjct: 491 LGLRMVQFMTATIVHAYDWSLPKGQECQKLDMEEAYGLTLQRAVPLMVQPIPRLSHKAY 508
BLAST of Spg021976 vs. ExPASy Swiss-Prot
Match:
Q7G602 (Flavonoid 3'-monooxygenase CYP75B3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP75B3 PE=2 SV=1)
HSP 1 Score: 563.5 bits (1451), Expect = 4.1e-159
Identity = 297/565 (52.57%), Postives = 383/565 (67.79%), Query Frame = 0
Query: 1 MDEISLIVAAAAVTLASAVLLHRFLFLRRSS------QSLRLPPGPKPWPIVGNLPQIGG 60
MD + L + ++ +++AV + FLR S + LPPGP+ WP++GNLPQ+G
Sbjct: 1 MDVVPLPLLLGSLAVSAAV-WYLVYFLRGGSGGDAARKRRPLPPGPRGWPVLGNLPQLGD 60
Query: 61 APLHSHLAAMAGEYGPLMYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIA 120
P H+ + A+A +YGPL LRFG +VVVAASA VA QFL+ D NF +RPPN+GA+++A
Sbjct: 61 KPHHT-MCALARQYGPLFRLRFGCAEVVVAASAPVAAQFLRGHDANFSNRPPNSGAEHVA 120
Query: 121 YNYQDMVFAPYGPRWRLLRKISAVHLFSSKALDDHRRVREEEVAVLVQTLASAGKAPATL 180
YNYQD+VFAPYG RWR LRK+ A+HLFS+KALDD R VRE EVA++V+ LA A L
Sbjct: 121 YNYQDLVFAPYGARWRALRKLCALHLFSAKALDDLRAVREGEVALMVRNLARQQAASVAL 180
Query: 181 GSLLTICTANALGRTMIGRRVFG-DGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSL 240
G +C N L R IG RVF DG +G +FK +V ++M L+G FN GDF P+L
Sbjct: 181 GQEANVCATNTLARATIGHRVFAVDGGEG----AREFKEMVVELMQLAGVFNVGDFVPAL 240
Query: 241 EWLDLLGVAGEMKKVHKWFDDFLTKILDEHRNLLAG------AGGSQHQDLLSMLLS--- 300
WLD GV +MK++H+ +D+ + ++E + AG A G DLLS+LL+
Sbjct: 241 RWLDPQGVVAKMKRLHRRYDNMMNGFINERK---AGAQPDGVAAGEHGNDLLSVLLARMQ 300
Query: 301 SKDQAEDENEKLSDTEIKALLLVLFLEQNLAAYWYYDFPTYPIKILATKRCGENNMFTAG 360
+ + + + EK+++T+IKALLL N+FTAG
Sbjct: 301 EEQKLDGDGEKITETDIKALLL--------------------------------NLFTAG 360
Query: 361 TDTSASTVEWALAELIRHPKMLAQTQQELDSVVGRDRLVSDLDLPRLGYLQAVVKETFRL 420
TDT++STVEWALAELIRHP +L + Q ELD+VVGR RLVS+ DLPRL YL AV+KETFRL
Sbjct: 361 TDTTSSTVEWALAELIRHPDVLKEAQHELDTVVGRGRLVSESDLPRLPYLTAVIKETFRL 420
Query: 421 HPPTPLSLPRMAAESCEINGFHIPKGSTLLVDVWAIGRDPKEWADPLEFRPNRFLPGGEK 480
HP TPLSLPR AAE CE++G+ IPKG+TLLV+VWAI RDP +W DPL+++P+RFLPG
Sbjct: 421 HPSTPLSLPREAAEECEVDGYRIPKGATLLVNVWAIARDPTQWPDPLQYQPSRFLPGRMH 480
Query: 481 AHVDVKGNDFEVIPFGAGRRICVGMSLGLRTVQMLTATLVHSFDWALPIGLSPDKLNMDE 540
A VDVKG DF +IPFGAGRRIC G+S GLR V ++TATLVH FDW L G +PDKLNM+E
Sbjct: 481 ADVDVKGADFGLIPFGAGRRICAGLSWGLRMVTLMTATLVHGFDWTLANGATPDKLNMEE 524
Query: 541 AFGLTLRRAQPLALHPRPRLSPEVY 550
A+GLTL+RA PL + P PRL P Y
Sbjct: 541 AYGLTLQRAVPLMVQPVPRLLPSAY 524
BLAST of Spg021976 vs. ExPASy Swiss-Prot
Match:
A0A4D6Q414 (Flavonoid 3',5'-hydroxylase CYP75B138 OS=Crocosmia x crocosmiiflora OX=1053288 GN=CYP75B138 PE=1 SV=1)
HSP 1 Score: 556.2 bits (1432), Expect = 6.5e-157
Identity = 284/559 (50.81%), Postives = 385/559 (68.87%), Query Frame = 0
Query: 1 MDEISLIVAAAAVTLASAVLLHRFLFLRRSSQSLRLPPGPKPWPIVGNLPQIGGAPLHSH 60
M + +I+ +A+ S + F +RS+ L+LPPGPK +P++GNLPQ+G P H
Sbjct: 3 MTSLDIILFISAIVFLSIYYYNLFSNAKRSN-GLKLPPGPKGYPVLGNLPQLGAKP-HQA 62
Query: 61 LAAMAGEYGPLMYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDM 120
L A + YGPLM LR GSVD+VVA+S +VA QFLK D NF +RPPN+GA+++A+NY D+
Sbjct: 63 LQAFSRVYGPLMRLRLGSVDLVVASSPSVAAQFLK-NDSNFCARPPNSGAEHMAFNYHDL 122
Query: 121 VFAPYGPRWRLLRKISAVHLFSSKALDDHRRVREEEVAVLVQTL--ASAGKAPATLGSLL 180
VFAPYGPRWRLLRK+SAVHL KALDD++ VREEE+AVL + L S G P +G +
Sbjct: 123 VFAPYGPRWRLLRKLSAVHLLGPKALDDNQNVREEELAVLARMLYERSRGGEPVNVGKEM 182
Query: 181 TICTANALGRTMIGRRVFGDGSDGGD--------PKVDQFKAIVEQVMVLSGKFNPGDFF 240
+C+ NAL R M+GRRVF + GG K ++FK +V +VM L+G FN GDF
Sbjct: 183 HVCSTNALSRAMMGRRVFEKLAVGGGGVEEEEEMKKAEEFKDMVVEVMTLAGVFNIGDFV 242
Query: 241 PSLEWLDLLGVAGEMKKVHKWFDDFLTKILDEHRNLLAGAGGSQHQDLLSMLLSSKDQAE 300
P L+ D+ GV +MK+VH+ ++ FL K + E R+ A G + DLLS+L+ + +++
Sbjct: 243 PWLKPFDIQGVVRKMKRVHRRYNVFLDKFIAECRS-SAKPGAN---DLLSVLIGQRGKSD 302
Query: 301 DENEKLSDTEIKALLLVLFLEQNLAAYWYYDFPTYPIKILATKRCGENNMFTAGTDTSAS 360
+++DT IKAL+L N+ TAGTDTS+S
Sbjct: 303 GSGGEITDTAIKALVL--------------------------------NLLTAGTDTSSS 362
Query: 361 TVEWALAELIRHPKMLAQTQQELDSVVGRDRLVSDLDLPRLGYLQAVVKETFRLHPPTPL 420
T+EWAL ELIRHP +L + QQE+DS VGRDRLV++ D+P+L YLQA+VKE FR+HP TPL
Sbjct: 363 TIEWALTELIRHPDILKKAQQEIDSAVGRDRLVTESDVPKLPYLQAIVKENFRMHPATPL 422
Query: 421 SLPRMAAESCEINGFHIPKGSTLLVDVWAIGRDPKEWADPLEFRPNRFLPGGEKAHVDVK 480
SLPRM+ E C+I G+HIPK STL V++WA+GRDP W DP+EFRP+RFLPGG+ H++V+
Sbjct: 423 SLPRMSIEECDIGGYHIPKNSTLFVNIWAMGRDPSIWPDPMEFRPSRFLPGGQGEHLEVR 482
Query: 481 GNDFEVIPFGAGRRICVGMSLGLRTVQMLTATLVHSFDWALPIGLSPDKLNMDEAFGLTL 540
GN FE++PFGAGRRIC G S+G+R V ATL+H+FDW LP GL+ +K++M+EAFG++L
Sbjct: 483 GNHFELMPFGAGRRICAGTSMGIRVVHSTVATLIHAFDWKLPEGLTAEKIDMEEAFGISL 522
Query: 541 RRAQPLALHPRPRLSPEVY 550
++A PL HP PRL+P+ Y
Sbjct: 543 QKAIPLMAHPIPRLAPKAY 522
BLAST of Spg021976 vs. ExPASy TrEMBL
Match:
A0A6J1EZJ7 (flavonoid 3'-monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111440987 PE=3 SV=1)
HSP 1 Score: 900.6 bits (2326), Expect = 5.2e-258
Identity = 451/550 (82.00%), Postives = 483/550 (87.82%), Query Frame = 0
Query: 1 MDEISLIVAAAAVTLASAVLLHRFLFLRRSSQSLRLPPGPKPWPIVGNLPQIGGAPLHSH 60
MDEISL +AA+TLAS VLL+R LF +R+S+SL LPPGPKPWPIVGNLPQIGGAPLHS
Sbjct: 1 MDEISLFF-SAAITLASVVLLYRLLFHQRNSRSLPLPPGPKPWPIVGNLPQIGGAPLHSL 60
Query: 61 LAAMAGEYGPLMYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDM 120
LA M+GEYGPLMYLRFGSVDVVVAASAA+AEQFLKIRD NFLSRPPNAGAKYIAYNYQDM
Sbjct: 61 LADMSGEYGPLMYLRFGSVDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDM 120
Query: 121 VFAPYGPRWRLLRKISAVHLFSSKALDDHRRVREEEVAVLVQTLASAGKAPATLGSLLTI 180
VFAPYGPRWRLLRKISAVHLFSSKAL+DHRRVREEEV VLVQ LA +G+APATLGSLLT+
Sbjct: 121 VFAPYGPRWRLLRKISAVHLFSSKALEDHRRVREEEVGVLVQALARSGEAPATLGSLLTV 180
Query: 181 CTANALGRTMIGRRVFGDGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLDLLG 240
CTANALGRTMIGRRVFGDGS GGDP DQFKA+VEQVM+LSGKFNPGDFF LEWLDLLG
Sbjct: 181 CTANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGDFFSWLEWLDLLG 240
Query: 241 VAGEMKKVHKWFDDFLTKILDEHRNLLAGAGGSQHQDLLSMLLSSKDQAEDENEKLSDTE 300
VAGEMKKVHKWFDD ++KIL+EHRNLLA GG +HQDLLS LLS++D+A+DENEKL+DTE
Sbjct: 241 VAGEMKKVHKWFDDLMSKILEEHRNLLATGGGGEHQDLLSTLLSTRDRAKDENEKLNDTE 300
Query: 301 IKALLLVLFLEQNLAAYWYYDFPTYPIKILATKRCGENNMFTAGTDTSASTVEWALAELI 360
IKALLL NMFTAGTDTSASTVEWALAELI
Sbjct: 301 IKALLL--------------------------------NMFTAGTDTSASTVEWALAELI 360
Query: 361 RHPKMLAQTQQELDSVVGRDRLVSDLDLPRLGYLQAVVKETFRLHPPTPLSLPRMAAESC 420
RHPKM+ Q QQELDSVVGRDR+VSDLDLP+LGYLQAVVKETFRLHPPTPLSLPRMAAESC
Sbjct: 361 RHPKMMYQAQQELDSVVGRDRVVSDLDLPKLGYLQAVVKETFRLHPPTPLSLPRMAAESC 420
Query: 421 EINGFHIPKGSTLLVDVWAIGRDPKEWADPLEFRPNRFLPGGEKAHVDVKGNDFEVIPFG 480
EI+G+HIPKGSTLLVDVWAI RDPKEW PLEFRP+RFLPGGEKAHVDVKGNDF VIPFG
Sbjct: 421 EISGYHIPKGSTLLVDVWAISRDPKEWTSPLEFRPDRFLPGGEKAHVDVKGNDFGVIPFG 480
Query: 481 AGRRICVGMSLGLRTVQMLTATLVHSFDWALPIGLSPDKLNMDEAFGLTLRRAQPLALHP 540
AGRRICVGMS+GLR VQMLTA+LVHSFDWALP GLSP +LNMDEAFGLTLRRAQPL HP
Sbjct: 481 AGRRICVGMSMGLRMVQMLTASLVHSFDWALPSGLSPGELNMDEAFGLTLRRAQPLVAHP 517
Query: 541 RPRLSPEVYQ 551
RPRLSP VYQ
Sbjct: 541 RPRLSPGVYQ 517
BLAST of Spg021976 vs. ExPASy TrEMBL
Match:
A0A6J1L0D6 (flavonoid 3'-monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111499893 PE=3 SV=1)
HSP 1 Score: 888.6 bits (2295), Expect = 2.0e-254
Identity = 448/550 (81.45%), Postives = 479/550 (87.09%), Query Frame = 0
Query: 1 MDEISLIVAAAAVTLASAVLLHRFLFLRRSSQSLRLPPGPKPWPIVGNLPQIGGAPLHSH 60
MDEISL +AA+TLA+ VLL+R LF +R+S+SL LPPGPKPWP VGNLPQIGGAPLHS
Sbjct: 1 MDEISLFF-SAAITLATVVLLYRLLFHQRNSRSLPLPPGPKPWPTVGNLPQIGGAPLHSQ 60
Query: 61 LAAMAGEYGPLMYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDM 120
LAAM+GEYGPLMYLRFGS+DVVVAASAA+AEQFLKIRD NFLSRPPNAGAKYIAYNYQDM
Sbjct: 61 LAAMSGEYGPLMYLRFGSIDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDM 120
Query: 121 VFAPYGPRWRLLRKISAVHLFSSKALDDHRRVREEEVAVLVQTLASAGKAPATLGSLLTI 180
VFAPYGPRWRLLRKISAVHLFSSKAL+D+R VREEEV VLVQ LA G+APATLGSLLT+
Sbjct: 121 VFAPYGPRWRLLRKISAVHLFSSKALEDNRCVREEEVGVLVQALARPGEAPATLGSLLTV 180
Query: 181 CTANALGRTMIGRRVFGDGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLDLLG 240
CTANALGRTMIGRRVFGDGS GGDP DQFKA+VEQVM+LSGKFNPG+FFPSLEWLDLLG
Sbjct: 181 CTANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGNFFPSLEWLDLLG 240
Query: 241 VAGEMKKVHKWFDDFLTKILDEHRNLLAGAGGSQHQDLLSMLLSSKDQAEDENEKLSDTE 300
VA EMKKVHKWFDD L+KIL+EHR LL A G +HQDLLS LLS++D AEDENEKL+DTE
Sbjct: 241 VAREMKKVHKWFDDLLSKILEEHRTLL--ASGGEHQDLLSTLLSTRDHAEDENEKLNDTE 300
Query: 301 IKALLLVLFLEQNLAAYWYYDFPTYPIKILATKRCGENNMFTAGTDTSASTVEWALAELI 360
IKALLL NMFTAGTDTSASTVEWALAELI
Sbjct: 301 IKALLL--------------------------------NMFTAGTDTSASTVEWALAELI 360
Query: 361 RHPKMLAQTQQELDSVVGRDRLVSDLDLPRLGYLQAVVKETFRLHPPTPLSLPRMAAESC 420
RHPKM+ Q QQELDSVVGRDR+VSDLDLP+LGYLQAVVKETFRLHPPTPLSLPRMAAESC
Sbjct: 361 RHPKMMYQAQQELDSVVGRDRVVSDLDLPKLGYLQAVVKETFRLHPPTPLSLPRMAAESC 420
Query: 421 EINGFHIPKGSTLLVDVWAIGRDPKEWADPLEFRPNRFLPGGEKAHVDVKGNDFEVIPFG 480
EING+HIPKGSTLLVDVWAI RDPKEW PLEFRP+RFLPGGEKAHVDVKGNDF VIPFG
Sbjct: 421 EINGYHIPKGSTLLVDVWAISRDPKEWTCPLEFRPDRFLPGGEKAHVDVKGNDFGVIPFG 480
Query: 481 AGRRICVGMSLGLRTVQMLTATLVHSFDWALPIGLSPDKLNMDEAFGLTLRRAQPLALHP 540
AGRRICVGMS+GLR VQMLTA+LVHSFDWALP GLSP +LNMDEAFGLTLRRAQPL HP
Sbjct: 481 AGRRICVGMSMGLRMVQMLTASLVHSFDWALPSGLSPGELNMDEAFGLTLRRAQPLVAHP 515
Query: 541 RPRLSPEVYQ 551
RPRLSP VYQ
Sbjct: 541 RPRLSPGVYQ 515
BLAST of Spg021976 vs. ExPASy TrEMBL
Match:
A0A5A7UTD5 (Flavonoid 3'-monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002910 PE=3 SV=1)
HSP 1 Score: 854.4 bits (2206), Expect = 4.3e-244
Identity = 433/557 (77.74%), Postives = 470/557 (84.38%), Query Frame = 0
Query: 1 MDEISLIVAAAAVTLASAVLLHRFLFLRRSSQSLRLPPGPKPWPIVGNLPQIGGAPLHSH 60
M+EI+LIV AA +TLASA LLHR FLRR RLPPGPKPWPIVGN+PQIGGAPLHS
Sbjct: 1 MEEITLIV-AAIITLASAFLLHRTFFLRR---RFRLPPGPKPWPIVGNMPQIGGAPLHSL 60
Query: 61 LAAMAGEYGPLMYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDM 120
LAAMAG+YGPLMYLR GSVD+VVAASAAVAEQ LKI D NFLSRPPNAGAKYIAYNYQDM
Sbjct: 61 LAAMAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDM 120
Query: 121 VFAPYGPRWRLLRKISAVHLFSSKALDDHRRVREEEVAVLVQTLASAGKAPATLGSLLTI 180
VFAPYGPRWRLLRKISAVHLFSSKALDDHRR+R++E++VLVQ LA +G+APA LGSLLT+
Sbjct: 121 VFAPYGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTV 180
Query: 181 CTANALGRTMIGRRVFGDGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLDLLG 240
C AN+LGRTMIGRRVFGDGS D + +QFKA+VEQVMVL+GKFNPGDFFP LE LDL+G
Sbjct: 181 CIANSLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMG 240
Query: 241 VAGEMKKVHKWFDDFLTKILDEHRNLLA----GAGGSQHQDLLSMLLSSKDQAEDENEKL 300
V EMKKVHKWFDDFLTKI++EHRNLLA G+GG HQDLLS LLS KD+ DENEKL
Sbjct: 241 VGSEMKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKL 300
Query: 301 SDTEIKALLLVLFLEQNLAAYWYYDFPTYPIKILATKRCGENNMFTAGTDTSASTVEWAL 360
+DTEIKALLL NMFTAGTDTSASTVEWAL
Sbjct: 301 NDTEIKALLL--------------------------------NMFTAGTDTSASTVEWAL 360
Query: 361 AELIRHPKMLAQTQQELDSVVGRDRLVSDLDLPRLGYLQAVVKETFRLHPPTPLSLPRMA 420
ELIRHP+M+AQ QQELDSVVGRDR VSDLDLP+L YLQAVVKETFRLHPPTPLSLPRMA
Sbjct: 361 TELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQLVYLQAVVKETFRLHPPTPLSLPRMA 420
Query: 421 AESCEINGFHIPKGSTLLVDVWAIGRDPKEWADPLEFRPNRFLPGGEKAHVDVKGNDFEV 480
++SCE+NG+HIPKGSTLLVDVWAIGRDPKEW DPLEF PNRFLP GEK HVDVKGNDFE
Sbjct: 421 SDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPLEFGPNRFLPDGEKPHVDVKGNDFEA 480
Query: 481 IPFGAGRRICVGMSLGLRTVQMLTATLVHSFDWALPIGLSPDKLNMDEAFGLTLRRAQPL 540
IPFGAGRRICVG+SLGLR VQMLTAT+VHSFDWALP GL+PDKLNMDE +GLTLRRAQPL
Sbjct: 481 IPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWALPNGLTPDKLNMDEHYGLTLRRAQPL 521
Query: 541 ALHPRPRLSPEVYQGLP 554
+HPRPRL VYQ LP
Sbjct: 541 VVHPRPRLLLGVYQKLP 521
BLAST of Spg021976 vs. ExPASy TrEMBL
Match:
A0A1S3BVU8 (flavonoid 3'-monooxygenase OS=Cucumis melo OX=3656 GN=LOC103494036 PE=3 SV=1)
HSP 1 Score: 854.4 bits (2206), Expect = 4.3e-244
Identity = 433/557 (77.74%), Postives = 470/557 (84.38%), Query Frame = 0
Query: 1 MDEISLIVAAAAVTLASAVLLHRFLFLRRSSQSLRLPPGPKPWPIVGNLPQIGGAPLHSH 60
M+EI+LIV AA +TLASA LLHR FLRR RLPPGPKPWPIVGN+PQIGGAPLHS
Sbjct: 1 MEEITLIV-AAIITLASAFLLHRTFFLRR---RFRLPPGPKPWPIVGNMPQIGGAPLHSL 60
Query: 61 LAAMAGEYGPLMYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDM 120
LAAMAG+YGPLMYLR GSVD+VVAASAAVAEQ LKI D NFLSRPPNAGAKYIAYNYQDM
Sbjct: 61 LAAMAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDM 120
Query: 121 VFAPYGPRWRLLRKISAVHLFSSKALDDHRRVREEEVAVLVQTLASAGKAPATLGSLLTI 180
VFAPYGPRWRLLRKISAVHLFSSKALDDHRR+R++E++VLVQ LA +G+APA LGSLLT+
Sbjct: 121 VFAPYGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTV 180
Query: 181 CTANALGRTMIGRRVFGDGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLDLLG 240
C AN+LGRTMIGRRVFGDGS D + +QFKA+VEQVMVL+GKFNPGDFFP LE LDL+G
Sbjct: 181 CIANSLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMG 240
Query: 241 VAGEMKKVHKWFDDFLTKILDEHRNLLA----GAGGSQHQDLLSMLLSSKDQAEDENEKL 300
V EMKKVHKWFDDFLTKI++EHRNLLA G+GG HQDLLS LLS KD+ DENEKL
Sbjct: 241 VGSEMKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKL 300
Query: 301 SDTEIKALLLVLFLEQNLAAYWYYDFPTYPIKILATKRCGENNMFTAGTDTSASTVEWAL 360
+DTEIKALLL NMFTAGTDTSASTVEWAL
Sbjct: 301 NDTEIKALLL--------------------------------NMFTAGTDTSASTVEWAL 360
Query: 361 AELIRHPKMLAQTQQELDSVVGRDRLVSDLDLPRLGYLQAVVKETFRLHPPTPLSLPRMA 420
ELIRHP+M+AQ QQELDSVVGRDR VSDLDLP+L YLQAVVKETFRLHPPTPLSLPRMA
Sbjct: 361 TELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQLVYLQAVVKETFRLHPPTPLSLPRMA 420
Query: 421 AESCEINGFHIPKGSTLLVDVWAIGRDPKEWADPLEFRPNRFLPGGEKAHVDVKGNDFEV 480
++SCE+NG+HIPKGSTLLVDVWAIGRDPKEW DPLEF PNRFLP GEK HVDVKGNDFE
Sbjct: 421 SDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPLEFGPNRFLPDGEKPHVDVKGNDFEA 480
Query: 481 IPFGAGRRICVGMSLGLRTVQMLTATLVHSFDWALPIGLSPDKLNMDEAFGLTLRRAQPL 540
IPFGAGRRICVG+SLGLR VQMLTAT+VHSFDWALP GL+PDKLNMDE +GLTLRRAQPL
Sbjct: 481 IPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWALPNGLTPDKLNMDEHYGLTLRRAQPL 521
Query: 541 ALHPRPRLSPEVYQGLP 554
+HPRPRL VYQ LP
Sbjct: 541 VVHPRPRLLLGVYQKLP 521
BLAST of Spg021976 vs. ExPASy TrEMBL
Match:
A0A0A0LP99 (Flavonoid 3' hydroxylase OS=Cucumis sativus OX=3659 GN=Csa_1G013190 PE=3 SV=1)
HSP 1 Score: 765.4 bits (1975), Expect = 2.6e-217
Identity = 392/542 (72.32%), Postives = 429/542 (79.15%), Query Frame = 0
Query: 1 MDEISLIVAAAAVTLASAVLLHRFLFLRRSSQSLRLPPGPKPWPIVGNLPQIGGAPLHSH 60
M+EI+ IV AA +TLASA LLHR FLRR LRLPPGPKPWPIVGN+PQIG A LHS
Sbjct: 1 MEEITPIV-AALITLASAFLLHRAFFLRR---RLRLPPGPKPWPIVGNMPQIGEAHLHSL 60
Query: 61 LAAMAGEYGPLMYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDM 120
LA MAG+YGPLMYLR GSVD+VVAASAAVAEQ LKI D NFLSRPPNAGAKYIAYNYQDM
Sbjct: 61 LATMAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDM 120
Query: 121 VFAPYGPRWRLLRKISAVHLFSSKALDDHRRVREEEVAVLVQTLASAGKAPATLGSLLTI 180
VFAPYGPRWRLLRKIS VHLFSSKALDDHRR+REEE++VLVQ LA +G+APA LGSLLT+
Sbjct: 121 VFAPYGPRWRLLRKISTVHLFSSKALDDHRRIREEEISVLVQALARSGEAPANLGSLLTV 180
Query: 181 CTANALGRTMIGRRVFGDGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLDLLG 240
C AN+LGRTMIGRRVFGDGS D + +QFK +VEQVMVL+GKFNPGDFFP LEWLDL+G
Sbjct: 181 CIANSLGRTMIGRRVFGDGSGSDDLESNQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMG 240
Query: 241 VAGEMKKVHKWFDDFLTKILDEHRNLLAGAGGSQHQDLLSMLLSSKDQAEDENEKLSDTE 300
V EMKKVHKWFDDFLTKI++EHRNLLA G HQDLLS LLS KD EDENEKL+DTE
Sbjct: 241 VGREMKKVHKWFDDFLTKIVEEHRNLLARGVGGGHQDLLSTLLSMKDDGEDENEKLNDTE 300
Query: 301 IKALLLVLFLEQNLAAYWYYDFPTYPIKILATKRCGENNMFTAGTDTSASTVEWALAELI 360
IKALLLVLFL + +Y
Sbjct: 301 IKALLLVLFLSK------FY---------------------------------------- 360
Query: 361 RHPKMLAQTQQELDSVVGRDRLVSDLDLPRLGYLQAVVKETFRLHPPTPLSLPRMAAESC 420
L+ +GRDR VSD+DL +L YLQAVVKETFRLHPPTPLSLPRMA++SC
Sbjct: 361 ------------LNCFIGRDRAVSDVDLHQLVYLQAVVKETFRLHPPTPLSLPRMASDSC 420
Query: 421 EINGFHIPKGSTLLVDVWAIGRDPKEWADPLEFRPNRFLPGGEKAHVDVKGNDFEVIPFG 480
E+NG+HIPKGSTLLVDVWAIGRDPK+W DPLEFRPNRFLP GEK HVDVKGNDFEVIPFG
Sbjct: 421 EVNGYHIPKGSTLLVDVWAIGRDPKQWVDPLEFRPNRFLPNGEKPHVDVKGNDFEVIPFG 480
Query: 481 AGRRICVGMSLGLRTVQMLTATLVHSFDWALPIGLSPDKLNMDEAFGLTLRRAQPLALHP 540
AGRRICVG+SLGLR VQMLTAT+VHSFDW LP GL+PDKLNMDE +GLTLRRAQPL +HP
Sbjct: 481 AGRRICVGLSLGLRMVQMLTATIVHSFDWTLPNGLTPDKLNMDEHYGLTLRRAQPLIMHP 480
Query: 541 RP 543
RP
Sbjct: 541 RP 480
BLAST of Spg021976 vs. TAIR 10
Match:
AT5G07990.1 (Cytochrome P450 superfamily protein )
HSP 1 Score: 639.4 bits (1648), Expect = 4.1e-183
Identity = 321/545 (58.90%), Postives = 396/545 (72.66%), Query Frame = 0
Query: 14 TLASAVLLHRFLFL--------RRSSQSLRLPPGPKPWPIVGNLPQIGGAPLHSHLAAMA 73
TL +LL LFL R S + RLPPGP PWPI+GNLP +G P H L+AM
Sbjct: 3 TLFLTILLATVLFLILRIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMGTKP-HRTLSAMV 62
Query: 74 GEYGPLMYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDMVFAPY 133
YGP+++LR G VDVVVAAS +VAEQFLKI D NF SRPPN+GAK++AYNYQD+VFAPY
Sbjct: 63 TTYGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAPY 122
Query: 134 GPRWRLLRKISAVHLFSSKALDDHRRVREEEVAVLVQTLASAGKAPATLGSLLTICTANA 193
G RWRLLRKIS+VHLFS+KAL+D + VR+EEV L + L G P LG L+ +C NA
Sbjct: 123 GHRWRLLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKPVNLGQLVNMCVVNA 182
Query: 194 LGRTMIGRRVFGDGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLDLLGVAGEM 253
LGR MIGRR+FG + D K D+F+++V ++M L+G FN GDF PSL+WLDL GVAG+M
Sbjct: 183 LGREMIGRRLFGADA---DHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGKM 242
Query: 254 KKVHKWFDDFLTKILDEHRNLLAGAGGSQHQDLLSMLLSSK-DQAEDENEKLSDTEIKAL 313
K++HK FD FL+ IL EH +H D+LS L+S K + + L+DTEIKAL
Sbjct: 243 KRLHKRFDAFLSSILKEHE---MNGQDQKHTDMLSTLISLKGTDLDGDGGSLTDTEIKAL 302
Query: 314 LLVLFLEQNLAAYWYYDFPTYPIKILATKRCGENNMFTAGTDTSASTVEWALAELIRHPK 373
LL NMFTAGTDTSASTV+WA+AELIRHP
Sbjct: 303 LL--------------------------------NMFTAGTDTSASTVDWAIAELIRHPD 362
Query: 374 MLAQTQQELDSVVGRDRLVSDLDLPRLGYLQAVVKETFRLHPPTPLSLPRMAAESCEING 433
++ + Q+ELD VVGRDR V++ D+ +L YLQAV+KE FRLHPPTPLSLP +A+ESCEING
Sbjct: 363 IMVKAQEELDIVVGRDRPVNESDIAQLPYLQAVIKENFRLHPPTPLSLPHIASESCEING 422
Query: 434 FHIPKGSTLLVDVWAIGRDPKEWADPLEFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRR 493
+HIPKGSTLL ++WAI RDP +W+DPL F+P RFLPGGEK+ VDVKG+DFE+IPFGAGRR
Sbjct: 423 YHIPKGSTLLTNIWAIARDPDQWSDPLAFKPERFLPGGEKSGVDVKGSDFELIPFGAGRR 482
Query: 494 ICVGMSLGLRTVQMLTATLVHSFDWALPIGLSPDKLNMDEAFGLTLRRAQPLALHPRPRL 550
IC G+SLGLRT+Q LTATLV FDW L G++P+KLNM+E++GLTL+RA PL +HP+PRL
Sbjct: 483 ICAGLSLGLRTIQFLTATLVQGFDWELAGGVTPEKLNMEESYGLTLQRAVPLVVHPKPRL 508
BLAST of Spg021976 vs. TAIR 10
Match:
AT1G01280.1 (cytochrome P450, family 703, subfamily A, polypeptide 2 )
HSP 1 Score: 328.2 bits (840), Expect = 2.0e-89
Identity = 193/549 (35.15%), Postives = 291/549 (53.01%), Query Frame = 0
Query: 6 LIVAAAAVTLASAVLLHRFLFLRRSSQSLRLPPGPKPWPIVGNLPQIGGAPLHSHLAAMA 65
L++A+ L VLL R+L + ++ RLPPGP PI+GNL Q+G P H LA++
Sbjct: 3 LVLASLFAVLILNVLLWRWL-KASACKAQRLPPGPPRLPILGNLLQLGPLP-HRDLASLC 62
Query: 66 GEYGPLMYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDMVFAPY 125
+YGPL+YLR G+VD + + L +D F SRP A ++AY D+ AP
Sbjct: 63 DKYGPLVYLRLGNVDAITTNDPDTIREILLRQDDVFSSRPKTLAAVHLAYGCGDVALAPM 122
Query: 126 GPRWRLLRKISAVHLFSSKALDDHRRVREEEVAVLVQTL---ASAGKAPATLGSLLTICT 185
GP W+ +R+I HL ++K L+ R EE L++ + + GK P L +L +
Sbjct: 123 GPHWKRMRRICMEHLLTTKRLESFTTQRAEEARYLIRDVFKRSETGK-PINLKEVLGAFS 182
Query: 186 ANALGRTMIGRRVFGDGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLDLLGVA 245
N + R ++G++ FG GS + +F I ++ L G GD+ P W+D G
Sbjct: 183 MNNVTRMLLGKQFFGPGSLVSPKEAQEFLHITHKLFWLLGVIYLGDYLPFWRWVDPSGCE 242
Query: 246 GEMKKVHKWFDDFLTKILDEHRNL-LAGAGGSQHQDLLSMLLSSKDQAEDENEKLSDTEI 305
EM+ V K D+F TKI+DEHR L + D + +LLS E+ + D EI
Sbjct: 243 KEMRDVEKRVDEFHTKIIDEHRRAKLEDEDKNGDMDFVDVLLSL--PGENGKAHMEDVEI 302
Query: 306 KALLLVLFLEQNLAAYWYYDFPTYPIKILATKRCGENNMFTAGTDTSASTVEWALAELIR 365
KAL+ +M A TDTSA T EWA+AE I+
Sbjct: 303 KALI--------------------------------QDMIAAATDTSAVTNEWAMAEAIK 362
Query: 366 HPKMLAQTQQELDSVVGRDRLVSDLDLPRLGYLQAVVKETFRLHPPTPLSLPRMAAESCE 425
P+++ + Q+ELD+VVG +R+V + DL L YL+ VV+ETFR+HP P +P + +
Sbjct: 363 QPRVMRKIQEELDNVVGSNRMVDESDLVHLNYLRCVVRETFRMHPAGPFLIPHESVRATT 422
Query: 426 INGFHIPKGSTLLVDVWAIGRDPKEWADPLEFRPNRFLPGGEKAHVDVK-GNDFEVIPFG 485
ING++IP + + ++ +GR+ K W D +FRP R P V++ G DF+++PF
Sbjct: 423 INGYYIPAKTRVFINTHGLGRNTKIWDDVEDFRPERHWPVEGSGRVEISHGPDFKILPFS 482
Query: 486 AGRRICVGMSLGLRTVQMLTATLVHSFDWALPIGLSPDKLNMDEAFGLTLRRAQPLALHP 545
AG+R C G LG+ V M A L H F+W+ SP ++ E +G+T+ +A+PL
Sbjct: 483 AGKRKCPGAPLGVTMVLMALARLFHCFEWS-----SPGNIDTVEVYGMTMPKAKPLRAIA 509
Query: 546 RPRLSPEVY 550
+PRL+ +Y
Sbjct: 543 KPRLAAHLY 509
BLAST of Spg021976 vs. TAIR 10
Match:
AT4G22710.1 (cytochrome P450, family 706, subfamily A, polypeptide 2 )
HSP 1 Score: 322.4 bits (825), Expect = 1.1e-87
Identity = 190/547 (34.73%), Postives = 289/547 (52.83%), Query Frame = 0
Query: 11 AAVTLASAVLLH--RFLFLRRSSQSLR---LPPGPKPWPIVGNLPQIGGAPLHSHLAAMA 70
++V LA A LL +L+++ S+S + LPPGP P PIVGNLP + LH+ A+
Sbjct: 23 SSVMLAVAALLAVVCYLWIQGKSKSKKEPPLPPGPWPLPIVGNLPFLNSDVLHTQFQALT 82
Query: 71 GEYGPLMYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDMVFAPY 130
+YGPLM + GS +V +S +A + LK D+ F + K Y +D++++PY
Sbjct: 83 LKYGPLMKIHLGSKLAIVVSSPDMAREVLKTHDITFANHDLPEVGKINTYGGEDILWSPY 142
Query: 131 GPRWRLLRKISAVHLFSSKALDDHRRVREEEVAVLVQTLASAGK--APATLGSLLTICTA 190
G WR LRK+ + +F++ +L+ R EE + ++ + +P LG + +
Sbjct: 143 GTHWRRLRKLCVMKMFTTPSLEASYSTRREETRQTIVHMSEMAREGSPVNLGEQIFLSIF 202
Query: 191 NALGRTMIGRRVFGDGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLDLLGVAG 250
N + R M G V GD ++ K ++ + + G N DFFP D G+
Sbjct: 203 NVVTRMMWGATVEGDERTS---LGNELKTLISDISDIEGIQNYSDFFPLFSRFDFQGLVK 262
Query: 251 EMKKVHKWFDDFLTKILDEHRNLLAGAGGSQHQDLLSMLLSSKDQAEDENEKLSDTEIKA 310
+MK K D ++++ H ++ G + +D L LL KD +DE LS T +K+
Sbjct: 263 QMKGHVKKLDLLFDRVMESHVKMV-GKKSEEEEDFLQYLLRVKD--DDEKAPLSMTHVKS 322
Query: 311 LLLVLFLEQNLAAYWYYDFPTYPIKILATKRCGENNMFTAGTDTSASTVEWALAELIRHP 370
LL+ +M G DTS + E+A+AE++ P
Sbjct: 323 LLM--------------------------------DMVLGGVDTSVNASEFAMAEIVSRP 382
Query: 371 KMLAQTQQELDSVVGRDRLVSDLDLPRLGYLQAVVKETFRLHPPTPLSLPRMAAESCEIN 430
++L + + ELD VVG+D +V + LP+L YLQAV+KET RLHP PL +P +E+ +
Sbjct: 383 EVLNKIRLELDQVVGKDNIVEESHLPKLPYLQAVMKETLRLHPTLPLLVPHRNSETSVVA 442
Query: 431 GFHIPKGSTLLVDVWAIGRDPKEWADPLEFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGR 490
G+ +PK S + ++VWAI RDPK W +P EF+P RFL +D G DF+ +PFG+GR
Sbjct: 443 GYTVPKDSKIFINVWAIHRDPKNWDEPNEFKPERFLENS----LDFNGGDFKYLPFGSGR 502
Query: 491 RICVGMSLGLRTVQMLTATLVHSFDWALPIGLSPDKLNMDEAFGLTLRRAQPLALHPRPR 550
RIC +++ R V A+L+HSFDW P G K ++E FGL L+ PL P PR
Sbjct: 503 RICAAINMAERLVLFNIASLLHSFDWKAPQG---QKFEVEEKFGLVLKLKSPLVAIPVPR 524
BLAST of Spg021976 vs. TAIR 10
Match:
AT4G36220.1 (ferulic acid 5-hydroxylase 1 )
HSP 1 Score: 322.4 bits (825), Expect = 1.1e-87
Identity = 193/534 (36.14%), Postives = 281/534 (52.62%), Query Frame = 0
Query: 14 TLASAVLLHRFLFLRRSSQSLR--LPPGPKPWPIVGNLPQIGGAPLHSHLAAMAGEYGPL 73
T + +++ F+F+ ++ R PPGP+ WPI+GN+ + H LA +A +YG L
Sbjct: 16 TTSLVIVVSLFIFISFITRRRRPPYPPGPRGWPIIGNMLMMDQL-THRGLANLAKKYGGL 75
Query: 74 MYLRFGSVDVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDMVFAPYGPRWRL 133
+LR G + + +S VA Q L+++D F +RP Y+ Y+ DM FA YGP WR
Sbjct: 76 CHLRMGFLHMYAVSSPEVARQVLQVQDSVFSNRPATIAISYLTYDRADMAFAHYGPFWRQ 135
Query: 134 LRKISAVHLFSSKALDDHRRVREEEVAVLVQTLASAGKAPATLGSLLTICTANALGRTMI 193
+RK+ + +FS K + VR+E ++ + GK P +G + AL R +
Sbjct: 136 MRKVCVMKVFSRKRAESWASVRDEVDKMVRSVSCNVGK-PINVGEQIF-----ALTRNIT 195
Query: 194 GRRVFGDGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLDLLGVAGEMKKVHKW 253
R FG + G D+F I+++ L G FN DF P W+D G+ + K
Sbjct: 196 YRAAFGSACEKGQ---DEFIRILQEFSKLFGAFNVADFIPYFGWIDPQGINKRLVKARND 255
Query: 254 FDDFLTKILDEHRNLLAGAGGSQHQDLLSMLLSSKDQAEDENEKLSDTEIKALLLVLFLE 313
D F+ I+DEH M A D+ + + DT++ LL + E
Sbjct: 256 LDGFIDDIIDEH-----------------MKKKENQNAVDDGD-VVDTDMVDDLLAFYSE 315
Query: 314 QNLAAYWYYDFPTYPIKILATK-RCGENNMFTAGTDTSASTVEWALAELIRHPKMLAQTQ 373
+ D IK+ + ++ GT+T AS +EWAL EL+R P+ L + Q
Sbjct: 316 EAKLVSETADLQN-SIKLTRDNIKAIIMDVMFGGTETVASAIEWALTELLRSPEDLKRVQ 375
Query: 374 QELDSVVGRDRLVSDLDLPRLGYLQAVVKETFRLHPPTPLSLPRMAAESCEINGFHIPKG 433
QEL VVG DR V + D+ +L YL+ +KET R+HPP PL L AE I+GF IPK
Sbjct: 376 QELAEVVGLDRRVEESDIEKLTYLKCTLKETLRMHPPIPLLL-HETAEDTSIDGFFIPKK 435
Query: 434 STLLVDVWAIGRDPKEWADPLEFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMS 493
S ++++ +AIGRDP W DP FRP+RFL G D KG++FE IPFG+GRR C GM
Sbjct: 436 SRVMINAFAIGRDPTSWTDPDTFRPSRFLEPGVP---DFKGSNFEFIPFGSGRRSCPGMQ 495
Query: 494 LGLRTVQMLTATLVHSFDWALPIGLSPDKLNMDEAFGLTLRRAQPLALHPRPRL 545
LGL + + A ++H F W LP G+ P +L+M++ FGLT +A L P RL
Sbjct: 496 LGLYALDLAVAHILHCFTWKLPDGMKPSELDMNDVFGLTAPKATRLFAVPTTRL 516
BLAST of Spg021976 vs. TAIR 10
Match:
AT3G26330.1 (cytochrome P450, family 71, subfamily B, polypeptide 37 )
HSP 1 Score: 320.9 bits (821), Expect = 3.3e-87
Identity = 183/531 (34.46%), Postives = 285/531 (53.67%), Query Frame = 0
Query: 20 LLHRFLFLRRSSQSLRLPPGPKPWPIVGNLPQIGGAPLHSHLAAMAGEYGPLMYLRFGSV 79
LL L L++ Q R PP P +PI+GNL Q+G P H L +++ +YGP+M L+FGS+
Sbjct: 15 LLLAALRLKKRRQHQRKPPSPPGFPIIGNLHQLGELP-HQSLWSLSKKYGPVMLLKFGSI 74
Query: 80 DVVVAASAAVAEQFLKIRDVNFLSRPPNAGAKYIAYNYQDMVFAPYGPRWRLLRKISAVH 139
VV +S+ A+Q LKI D+N SRP AG + ++YNY D+VF+P+ W+ LR++
Sbjct: 75 PTVVVSSSETAKQALKIHDLNCCSRPSLAGPRALSYNYLDIVFSPFNDYWKELRRMCVQE 134
Query: 140 LFSSKALDDHRRVREEEVAVLVQTL--ASAGKAPATLGSLLTICTANALGRTMIGRRVFG 199
LFS K + + +REEEV L+ + ++A K P L L T +I + FG
Sbjct: 135 LFSPKQVHLIQPIREEEVKKLMNSFSESAAQKTPVNLSEKLASLTVG-----VICKAAFG 194
Query: 200 DGSDGGDPKVDQFKAIVEQVMVLSGKFNPGDFFPSLEWLD--LLGVAGEMKKVHKWFDDF 259
G D F ++ + G F+ D+FP++ W+ L G+ G+ ++ + D F
Sbjct: 195 VSFQGTVLNSDNFDKLIHDAFLFLGSFSASDYFPNVGWIIDWLTGLQGQRERSVRGLDAF 254
Query: 260 LTKILDEHRNLLAGAGGSQHQDLLSMLLS-SKDQAEDENEKLSDTEIKALLLVLFLEQNL 319
++ D H+ +D + +LL K++ KL+ IKA+L+
Sbjct: 255 YEQMFDLHKQ----GNKEGVEDFVDLLLKLEKEETVLGYGKLTRNHIKAVLM-------- 314
Query: 320 AAYWYYDFPTYPIKILATKRCGENNMFTAGTDTSASTVEWALAELIRHPKMLAQTQQELD 379
N+ G TSA T+ WA+ EL+R+P+++ + Q E+
Sbjct: 315 ------------------------NVLLGGIGTSAITMTWAMTELMRNPRVMKKVQSEIR 374
Query: 380 SVVGRDRLVSDLDLPRLGYLQAVVKETFRLHPPTPLSLPRMAAESCEINGFHIPKGSTLL 439
+ +G ++ D+ +L YL+ V+ ET+RLHPP PL +PR EING+ IP + L
Sbjct: 375 NQIGGKSMICLDDIDQLHYLKMVINETWRLHPPAPLLVPREVMSEFEINGYTIPAKTRLY 434
Query: 440 VDVWAIGRDPKEWADPLEFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSLGLR 499
V+VW IGRDP W DP EF P RF+ +++D KG +FE++PFG+GRR+C M +G
Sbjct: 435 VNVWGIGRDPDTWKDPEEFLPERFV----NSNIDAKGQNFELLPFGSGRRMCPAMYMGTT 494
Query: 500 TVQMLTATLVHSFDWALPIGLSPDKLNMDEAFGLTLRRAQPLALHPRPRLS 546
V+ A L++ FDW LP G+ + ++M+E+ GL + L L PR L+
Sbjct: 495 MVEFGLANLLYHFDWKLPEGMVVEDIDMEESPGLNASKKNELVLVPRKYLN 499
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_022933611.1 | 1.1e-257 | 82.00 | flavonoid 3'-monooxygenase [Cucurbita moschata] | [more] |
| KAG6587576.1 | 5.3e-257 | 81.82 | Flavonoid 3'-monooxygenase, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
| XP_023529494.1 | 1.2e-256 | 81.56 | flavonoid 3'-monooxygenase [Cucurbita pepo subsp. pepo] >XP_023529495.1 flavonoi... | [more] |
| XP_023007386.1 | 4.2e-254 | 81.45 | flavonoid 3'-monooxygenase [Cucurbita maxima] | [more] |
| XP_038878698.1 | 1.1e-249 | 80.47 | flavonoid 3'-monooxygenase [Benincasa hispida] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q9SBQ9 | 6.0e-187 | 59.96 | Flavonoid 3'-monooxygenase OS=Petunia hybrida OX=4102 GN=CYP75B2 PE=2 SV=1 | [more] |
| Q9SD85 | 5.8e-182 | 58.90 | Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP75B1 PE=1 SV=1 | [more] |
| A0A4D6Q415 | 3.3e-169 | 56.96 | Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN... | [more] |
| Q7G602 | 4.1e-159 | 52.57 | Flavonoid 3'-monooxygenase CYP75B3 OS=Oryza sativa subsp. japonica OX=39947 GN=C... | [more] |
| A0A4D6Q414 | 6.5e-157 | 50.81 | Flavonoid 3',5'-hydroxylase CYP75B138 OS=Crocosmia x crocosmiiflora OX=1053288 G... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1EZJ7 | 5.2e-258 | 82.00 | flavonoid 3'-monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111440987 PE=3 SV... | [more] |
| A0A6J1L0D6 | 2.0e-254 | 81.45 | flavonoid 3'-monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111499893 PE=3 SV=1 | [more] |
| A0A5A7UTD5 | 4.3e-244 | 77.74 | Flavonoid 3'-monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... | [more] |
| A0A1S3BVU8 | 4.3e-244 | 77.74 | flavonoid 3'-monooxygenase OS=Cucumis melo OX=3656 GN=LOC103494036 PE=3 SV=1 | [more] |
| A0A0A0LP99 | 2.6e-217 | 72.32 | Flavonoid 3' hydroxylase OS=Cucumis sativus OX=3659 GN=Csa_1G013190 PE=3 SV=1 | [more] |
