Spg021959 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg021959
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	EF hand calcium-binding protein family
Location	scaffold2: 3970383 .. 3970805 (-)
RNA-Seq Expression	Spg021959
Synteny	Spg021959

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTCCGACGGAAGCCTCACGATTCTGGAACTAGCGGCGTTGCTCCGATCGCTCGGTCTCAAGCCCTCCGGCGACCAAATCCACGTTCTTCTCGCTAATATGGACTCGAACGGCAATGGCTCGGTAGAGTTCGACGAATTGGTGACCGCGATTATGCCAGATTTCAACGAGGAGGTAATGGTAAATCAGACGCATCTTCTGGAGGTTTTCCGTTCGTTCGATCGAGACGGCAATGGATACATTACCGCGGCGGAGCTGGCTGGATCGATGGCGAAAATGGGACAGCCATTGACGTACAGAGAGCTAACGGAGATGATGAAGGAAGCCGATACGGATGGCGATGGCGTAATTAGCTTCAATGAATTTGCATCTGTAATGGCTAAATCGGCCGCTGAATTTCTAGGGCTTGCAATCTCTTGA

mRNA sequence

ATGGATTCCGACGGAAGCCTCACGATTCTGGAACTAGCGGCGTTGCTCCGATCGCTCGGTCTCAAGCCCTCCGGCGACCAAATCCACGTTCTTCTCGCTAATATGGACTCGAACGGCAATGGCTCGGTAGAGTTCGACGAATTGGTGACCGCGATTATGCCAGATTTCAACGAGGAGGTAATGGTAAATCAGACGCATCTTCTGGAGGTTTTCCGTTCGTTCGATCGAGACGGCAATGGATACATTACCGCGGCGGAGCTGGCTGGATCGATGGCGAAAATGGGACAGCCATTGACGTACAGAGAGCTAACGGAGATGATGAAGGAAGCCGATACGGATGGCGATGGCGTAATTAGCTTCAATGAATTTGCATCTGTAATGGCTAAATCGGCCGCTGAATTTCTAGGGCTTGCAATCTCTTGA

Coding sequence (CDS)

ATGGATTCCGACGGAAGCCTCACGATTCTGGAACTAGCGGCGTTGCTCCGATCGCTCGGTCTCAAGCCCTCCGGCGACCAAATCCACGTTCTTCTCGCTAATATGGACTCGAACGGCAATGGCTCGGTAGAGTTCGACGAATTGGTGACCGCGATTATGCCAGATTTCAACGAGGAGGTAATGGTAAATCAGACGCATCTTCTGGAGGTTTTCCGTTCGTTCGATCGAGACGGCAATGGATACATTACCGCGGCGGAGCTGGCTGGATCGATGGCGAAAATGGGACAGCCATTGACGTACAGAGAGCTAACGGAGATGATGAAGGAAGCCGATACGGATGGCGATGGCGTAATTAGCTTCAATGAATTTGCATCTGTAATGGCTAAATCGGCCGCTGAATTTCTAGGGCTTGCAATCTCTTGA

Protein sequence

MDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELVTAIMPDFNEEVMVNQTHLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISFNEFASVMAKSAAEFLGLAIS

Homology

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BLAST of Spg021959 vs. NCBI nr
Match: XP_004138337.2 (probable calcium-binding protein CML15 [Cucumis sativus] >XP_031741380.1 probable calcium-binding protein CML15 [Cucumis sativus])

HSP 1 Score: 266.5 bits (680), Expect = 1.3e-67
Identity = 138/140 (98.57%), Postives = 139/140 (99.29%), Query Frame = 0


Query: 1   MDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELVTAIMPDFNEEV 60
           MDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELVTAIMPDFNEEV
Sbjct: 21  MDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELVTAIMPDFNEEV 80

Query: 61  MVNQTHLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISF 120
           MVNQT LLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISF
Sbjct: 81  MVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISF 140

Query: 121 NEFASVMAKSAAEFLGLAIS 141
           NEFASVMAKSAA+FLGLAIS
Sbjct: 141 NEFASVMAKSAADFLGLAIS 160

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BLAST of Spg021959 vs. NCBI nr
Match: XP_008453344.1 (PREDICTED: probable calcium-binding protein CML15 [Cucumis melo] >KAA0058039.1 putative calcium-binding protein CML15 [Cucumis melo var. makuwa] >TYK28384.1 putative calcium-binding protein CML15 [Cucumis melo var. makuwa])

HSP 1 Score: 266.5 bits (680), Expect = 1.3e-67
Identity = 138/140 (98.57%), Postives = 139/140 (99.29%), Query Frame = 0


Query: 1   MDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELVTAIMPDFNEEV 60
           MDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELVTAIMPDFNEEV
Sbjct: 21  MDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELVTAIMPDFNEEV 80

Query: 61  MVNQTHLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISF 120
           MVNQT LLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISF
Sbjct: 81  MVNQTRLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISF 140

Query: 121 NEFASVMAKSAAEFLGLAIS 141
           NEFASVMAKSAA+FLGLAIS
Sbjct: 141 NEFASVMAKSAADFLGLAIS 160

[Close]

BLAST of Spg021959 vs. NCBI nr
Match: XP_038880166.1 (probable calcium-binding protein CML15 [Benincasa hispida])

HSP 1 Score: 266.5 bits (680), Expect = 1.3e-67
Identity = 138/140 (98.57%), Postives = 139/140 (99.29%), Query Frame = 0


Query: 1   MDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELVTAIMPDFNEEV 60
           MDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELVTAIMPDFNEEV
Sbjct: 21  MDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELVTAIMPDFNEEV 80

Query: 61  MVNQTHLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISF 120
           MVNQT LLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISF
Sbjct: 81  MVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISF 140

Query: 121 NEFASVMAKSAAEFLGLAIS 141
           NEFASVMAKSAA+FLGLAIS
Sbjct: 141 NEFASVMAKSAADFLGLAIS 160

[Close]

BLAST of Spg021959 vs. NCBI nr
Match: KAE8652452.1 (hypothetical protein Csa_013816 [Cucumis sativus])

HSP 1 Score: 266.5 bits (680), Expect = 1.3e-67
Identity = 138/140 (98.57%), Postives = 139/140 (99.29%), Query Frame = 0


Query: 1   MDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELVTAIMPDFNEEV 60
           MDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELVTAIMPDFNEEV
Sbjct: 117 MDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELVTAIMPDFNEEV 176

Query: 61  MVNQTHLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISF 120
           MVNQT LLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISF
Sbjct: 177 MVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISF 236

Query: 121 NEFASVMAKSAAEFLGLAIS 141
           NEFASVMAKSAA+FLGLAIS
Sbjct: 237 NEFASVMAKSAADFLGLAIS 256

[Close]

BLAST of Spg021959 vs. NCBI nr
Match: XP_022135250.1 (probable calcium-binding protein CML15 [Momordica charantia])

HSP 1 Score: 264.2 bits (674), Expect = 6.2e-67
Identity = 136/140 (97.14%), Postives = 139/140 (99.29%), Query Frame = 0


Query: 1   MDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELVTAIMPDFNEEV 60
           MDSDGSLTILELAALLRSLGLKPSGDQIHVL+ANMD+NGNGSVEFDELVTAIMPDFNEEV
Sbjct: 21  MDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELVTAIMPDFNEEV 80

Query: 61  MVNQTHLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISF 120
           MVNQT LLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISF
Sbjct: 81  MVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISF 140

Query: 121 NEFASVMAKSAAEFLGLAIS 141
           NEFASVMAKSA+EFLGLAIS
Sbjct: 141 NEFASVMAKSASEFLGLAIS 160

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BLAST of Spg021959 vs. ExPASy Swiss-Prot
Match: Q9FZ75 (Probable calcium-binding protein CML15 OS=Arabidopsis thaliana OX=3702 GN=CML15 PE=2 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 1.1e-58
Identity = 112/140 (80.00%), Postives = 129/140 (92.14%), Query Frame = 0


Query: 1   MDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELVTAIMPDFNEEV 60
           MD+DGSLTILELAALLRSLGLKPSGDQIHVLLA+MDSNGNG VEFDELV  I+PD NEEV
Sbjct: 17  MDADGSLTILELAALLRSLGLKPSGDQIHVLLASMDSNGNGFVEFDELVGTILPDLNEEV 76

Query: 61  MVNQTHLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISF 120
           ++N   LLE+F+SFDRDGNG+I+AAELAG+MAKMGQPLTY+ELTEM+KEADT+GDGVISF
Sbjct: 77  LINSEQLLEIFKSFDRDGNGFISAAELAGAMAKMGQPLTYKELTEMIKEADTNGDGVISF 136

Query: 121 NEFASVMAKSAAEFLGLAIS 141
            EFAS+MAKSA ++ GL I+
Sbjct: 137 GEFASIMAKSAVDYFGLKIN 156

[Close]

BLAST of Spg021959 vs. ExPASy Swiss-Prot
Match: Q9LI84 (Probable calcium-binding protein CML16 OS=Arabidopsis thaliana OX=3702 GN=CML16 PE=2 SV=2)

HSP 1 Score: 218.8 bits (556), Expect = 3.9e-56
Identity = 111/140 (79.29%), Postives = 123/140 (87.86%), Query Frame = 0


Query: 1   MDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELVTAIMPDFNEEV 60
           MD DGSLT LELAALLRSLG+KP GDQI +LL  +D NGNGSVEFDELV AI+PD NEEV
Sbjct: 22  MDKDGSLTQLELAALLRSLGIKPRGDQISLLLNQIDRNGNGSVEFDELVVAILPDINEEV 81

Query: 61  MVNQTHLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISF 120
           ++NQ  L+EVFRSFDRDGNG ITAAELAGSMAKMG PLTYRELTEMM EAD++GDGVISF
Sbjct: 82  LINQEQLMEVFRSFDRDGNGSITAAELAGSMAKMGHPLTYRELTEMMTEADSNGDGVISF 141

Query: 121 NEFASVMAKSAAEFLGLAIS 141
           NEF+ +MAKSAA+FLGL  S
Sbjct: 142 NEFSHIMAKSAADFLGLTAS 161

[Close]

BLAST of Spg021959 vs. ExPASy Swiss-Prot
Match: Q6L5F4 (Probable calcium-binding protein CML14 OS=Oryza sativa subsp. japonica OX=39947 GN=CML14 PE=2 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 2.7e-49
Identity = 99/139 (71.22%), Postives = 119/139 (85.61%), Query Frame = 0


Query: 1   MDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELVTAIMPDFNEEV 60
           M+ DGSLT LELAALLRSLGL+P+GD++H LLA MD+NGNGSVEFDEL  AI P    + 
Sbjct: 35  MNGDGSLTQLELAALLRSLGLRPTGDEVHALLAGMDANGNGSVEFDELAAAIAPVLTTQT 94

Query: 61  -MVNQTHLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVIS 120
            +V+Q  LLEVFR+FDRDGNG+I+AAELA SMA++GQPLT+ ELT MM++ADTDGDGVIS
Sbjct: 95  HLVDQAQLLEVFRAFDRDGNGFISAAELARSMARLGQPLTFEELTRMMRDADTDGDGVIS 154

Query: 121 FNEFASVMAKSAAEFLGLA 139
           F EFA+VMAKSA +FLG+A
Sbjct: 155 FKEFAAVMAKSALDFLGVA 173

[Close]

BLAST of Spg021959 vs. ExPASy Swiss-Prot
Match: Q5ZD81 (Probable calcium-binding protein CML12 OS=Oryza sativa subsp. japonica OX=39947 GN=CML12 PE=2 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 3.8e-43
Identity = 91/143 (63.64%), Postives = 115/143 (80.42%), Query Frame = 0


Query: 1   MDSDGSLTILELAALLRSLGLKP-SGDQIHVLLANMDSNGNGSVEFDELVTA----IMPD 60
           +D DGSLT LELAALLRSLGL+P +GD+IH L+A +D++GNG+VEFDEL ++    I+  
Sbjct: 105 LDGDGSLTKLELAALLRSLGLRPAAGDEIHALIAAIDADGNGTVEFDELASSLADLILGP 164

Query: 61  FNEEVMVNQTHLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGD 120
               V V+Q  L E FR+FDRDGNG+I+AAELA SMA+MG P+ Y ELT+MM+EADTDGD
Sbjct: 165 CRPSVAVDQAELAEAFRAFDRDGNGFISAAELARSMARMGHPICYAELTDMMREADTDGD 224

Query: 121 GVISFNEFASVMAKSAAEFLGLA 139
           G+ISF EF ++MAKSA +FLGLA
Sbjct: 225 GLISFEEFTAIMAKSALDFLGLA 247

[Close]

BLAST of Spg021959 vs. ExPASy Swiss-Prot
Match: Q9M8U1 (Probable calcium-binding protein CML18 OS=Arabidopsis thaliana OX=3702 GN=CML18 PE=1 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 1.2e-31
Identity = 74/130 (56.92%), Postives = 85/130 (65.38%), Query Frame = 0


Query: 2   DSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELVTAIMPDFNEEVM 61
           + DGSLT LEL +LLRSLGLKPS DQ+  L+   D N NG VEF E V  + PD   +  
Sbjct: 31  NKDGSLTELELGSLLRSLGLKPSQDQLDTLIQKADRNNNGLVEFSEFVALVEPDL-VKCP 90

Query: 62  VNQTHLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISFN 121
                L  +FR FDRDGNGYITAAELA SMAK+G  LT  ELT M+KEAD DGDG I F 
Sbjct: 91  YTDDQLKAIFRMFDRDGNGYITAAELAHSMAKLGHALTAEELTGMIKEADRDGDGCIDFQ 150

Query: 122 EFASVMAKSA 132
           EF   +  +A
Sbjct: 151 EFVQAITSAA 159

[Close]

BLAST of Spg021959 vs. ExPASy TrEMBL
Match: A0A5D3DXV7 (Putative calcium-binding protein CML15 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00110 PE=4 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 6.1e-68
Identity = 138/140 (98.57%), Postives = 139/140 (99.29%), Query Frame = 0


Query: 1   MDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELVTAIMPDFNEEV 60
           MDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELVTAIMPDFNEEV
Sbjct: 21  MDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELVTAIMPDFNEEV 80

Query: 61  MVNQTHLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISF 120
           MVNQT LLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISF
Sbjct: 81  MVNQTRLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISF 140

Query: 121 NEFASVMAKSAAEFLGLAIS 141
           NEFASVMAKSAA+FLGLAIS
Sbjct: 141 NEFASVMAKSAADFLGLAIS 160

[Close]

BLAST of Spg021959 vs. ExPASy TrEMBL
Match: A0A0A0LUS7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G015780 PE=4 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 6.1e-68
Identity = 138/140 (98.57%), Postives = 139/140 (99.29%), Query Frame = 0


Query: 1   MDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELVTAIMPDFNEEV 60
           MDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELVTAIMPDFNEEV
Sbjct: 21  MDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELVTAIMPDFNEEV 80

Query: 61  MVNQTHLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISF 120
           MVNQT LLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISF
Sbjct: 81  MVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISF 140

Query: 121 NEFASVMAKSAAEFLGLAIS 141
           NEFASVMAKSAA+FLGLAIS
Sbjct: 141 NEFASVMAKSAADFLGLAIS 160

[Close]

BLAST of Spg021959 vs. ExPASy TrEMBL
Match: A0A1S3BX63 (probable calcium-binding protein CML15 OS=Cucumis melo OX=3656 GN=LOC103494094 PE=4 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 6.1e-68
Identity = 138/140 (98.57%), Postives = 139/140 (99.29%), Query Frame = 0


Query: 1   MDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELVTAIMPDFNEEV 60
           MDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELVTAIMPDFNEEV
Sbjct: 21  MDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELVTAIMPDFNEEV 80

Query: 61  MVNQTHLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISF 120
           MVNQT LLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISF
Sbjct: 81  MVNQTRLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISF 140

Query: 121 NEFASVMAKSAAEFLGLAIS 141
           NEFASVMAKSAA+FLGLAIS
Sbjct: 141 NEFASVMAKSAADFLGLAIS 160

[Close]

BLAST of Spg021959 vs. ExPASy TrEMBL
Match: A0A6J1C0M6 (probable calcium-binding protein CML15 OS=Momordica charantia OX=3673 GN=LOC111007259 PE=4 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 3.0e-67
Identity = 136/140 (97.14%), Postives = 139/140 (99.29%), Query Frame = 0


Query: 1   MDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELVTAIMPDFNEEV 60
           MDSDGSLTILELAALLRSLGLKPSGDQIHVL+ANMD+NGNGSVEFDELVTAIMPDFNEEV
Sbjct: 21  MDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELVTAIMPDFNEEV 80

Query: 61  MVNQTHLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISF 120
           MVNQT LLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISF
Sbjct: 81  MVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISF 140

Query: 121 NEFASVMAKSAAEFLGLAIS 141
           NEFASVMAKSA+EFLGLAIS
Sbjct: 141 NEFASVMAKSASEFLGLAIS 160

[Close]

BLAST of Spg021959 vs. ExPASy TrEMBL
Match: A0A6J1L5D2 (probable calcium-binding protein CML15 OS=Cucurbita maxima OX=3661 GN=LOC111499274 PE=4 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 6.7e-67
Identity = 133/140 (95.00%), Postives = 139/140 (99.29%), Query Frame = 0


Query: 1   MDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELVTAIMPDFNEEV 60
           MDSDGSLTILELAALLRSLGLKPSGDQIHVL+ANMD+NGNGSVEFDELVTAI PDFNEE+
Sbjct: 21  MDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGNGSVEFDELVTAIRPDFNEEI 80

Query: 61  MVNQTHLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISF 120
           MVNQTHLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISF
Sbjct: 81  MVNQTHLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISF 140

Query: 121 NEFASVMAKSAAEFLGLAIS 141
           NEFAS+MAKSA++FLGLAIS
Sbjct: 141 NEFASIMAKSASDFLGLAIS 160

[Close]

BLAST of Spg021959 vs. TAIR 10
Match: AT1G18530.1 (EF hand calcium-binding protein family )

HSP 1 Score: 227.3 bits (578), Expect = 7.9e-60
Identity = 112/140 (80.00%), Postives = 129/140 (92.14%), Query Frame = 0


Query: 1   MDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELVTAIMPDFNEEV 60
           MD+DGSLTILELAALLRSLGLKPSGDQIHVLLA+MDSNGNG VEFDELV  I+PD NEEV
Sbjct: 17  MDADGSLTILELAALLRSLGLKPSGDQIHVLLASMDSNGNGFVEFDELVGTILPDLNEEV 76

Query: 61  MVNQTHLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISF 120
           ++N   LLE+F+SFDRDGNG+I+AAELAG+MAKMGQPLTY+ELTEM+KEADT+GDGVISF
Sbjct: 77  LINSEQLLEIFKSFDRDGNGFISAAELAGAMAKMGQPLTYKELTEMIKEADTNGDGVISF 136

Query: 121 NEFASVMAKSAAEFLGLAIS 141
            EFAS+MAKSA ++ GL I+
Sbjct: 137 GEFASIMAKSAVDYFGLKIN 156

[Close]

BLAST of Spg021959 vs. TAIR 10
Match: AT3G25600.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 218.8 bits (556), Expect = 2.8e-57
Identity = 111/140 (79.29%), Postives = 123/140 (87.86%), Query Frame = 0


Query: 1   MDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELVTAIMPDFNEEV 60
           MD DGSLT LELAALLRSLG+KP GDQI +LL  +D NGNGSVEFDELV AI+PD NEEV
Sbjct: 22  MDKDGSLTQLELAALLRSLGIKPRGDQISLLLNQIDRNGNGSVEFDELVVAILPDINEEV 81

Query: 61  MVNQTHLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISF 120
           ++NQ  L+EVFRSFDRDGNG ITAAELAGSMAKMG PLTYRELTEMM EAD++GDGVISF
Sbjct: 82  LINQEQLMEVFRSFDRDGNGSITAAELAGSMAKMGHPLTYRELTEMMTEADSNGDGVISF 141

Query: 121 NEFASVMAKSAAEFLGLAIS 141
           NEF+ +MAKSAA+FLGL  S
Sbjct: 142 NEFSHIMAKSAADFLGLTAS 161

[Close]

BLAST of Spg021959 vs. TAIR 10
Match: AT3G03000.1 (EF hand calcium-binding protein family )

HSP 1 Score: 137.5 bits (345), Expect = 8.2e-33
Identity = 74/130 (56.92%), Postives = 85/130 (65.38%), Query Frame = 0


Query: 2   DSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELVTAIMPDFNEEVM 61
           + DGSLT LEL +LLRSLGLKPS DQ+  L+   D N NG VEF E V  + PD   +  
Sbjct: 31  NKDGSLTELELGSLLRSLGLKPSQDQLDTLIQKADRNNNGLVEFSEFVALVEPDL-VKCP 90

Query: 62  VNQTHLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISFN 121
                L  +FR FDRDGNGYITAAELA SMAK+G  LT  ELT M+KEAD DGDG I F 
Sbjct: 91  YTDDQLKAIFRMFDRDGNGYITAAELAHSMAKLGHALTAEELTGMIKEADRDGDGCIDFQ 150

Query: 122 EFASVMAKSA 132
           EF   +  +A
Sbjct: 151 EFVQAITSAA 159

[Close]

BLAST of Spg021959 vs. TAIR 10
Match: AT1G32250.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 129.8 bits (325), Expect = 1.7e-30
Identity = 70/134 (52.24%), Postives = 87/134 (64.93%), Query Frame = 0


Query: 2   DSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELVTAIMPDF----N 61
           + DGSLT LEL +LLR+LG+KPS DQ   L+   D+  NG VEF E V  + P+      
Sbjct: 27  NKDGSLTQLELGSLLRALGVKPSPDQFETLIDKADTKSNGLVEFPEFVALVSPELLSPAK 86

Query: 62  EEVMVNQTHLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGV 121
                 +  LL +FR FD DGNG+ITAAELA SMAK+G  LT  ELT M+KEAD+DGDG 
Sbjct: 87  RTTPYTEEQLLRLFRIFDTDGNGFITAAELAHSMAKLGHALTVAELTGMIKEADSDGDGR 146

Query: 122 ISFNEFASVMAKSA 132
           I+F EFA  +  +A
Sbjct: 147 INFQEFAKAINSAA 160

[Close]

BLAST of Spg021959 vs. TAIR 10
Match: AT4G14640.1 (calmodulin 8 )

HSP 1 Score: 104.8 bits (260), Expect = 5.9e-23
Identity = 50/126 (39.68%), Postives = 81/126 (64.29%), Query Frame = 0


Query: 2   DSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELVTAIMPDFNEEVM 61
           D DG +T+ ELA ++RSL   P+  ++H ++  +DS+ NG++EF E +  +     E   
Sbjct: 24  DGDGCITVEELATVIRSLDQNPTEQELHDIITEIDSDSNGTIEFAEFLNLMAKKLQESDA 83

Query: 62  VNQTHLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISFN 121
             +  L E F+ FD+D NGYI+A+EL+  M  +G+ LT  E+ +M+KEAD DGDG ++++
Sbjct: 84  --EEELKEAFKVFDKDQNGYISASELSHVMINLGEKLTDEEVEQMIKEADLDGDGQVNYD 143

Query: 122 EFASVM 128
           EF  +M
Sbjct: 144 EFVKMM 147

The following BLAST results are available for this feature:

BLAST of Spg021959 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004138337.2	1.3e-67	98.57	probable calcium-binding protein CML15 [Cucumis sativus] >XP_031741380.1 probabl...	[more]
XP_008453344.1	1.3e-67	98.57	PREDICTED: probable calcium-binding protein CML15 [Cucumis melo] >KAA0058039.1 p...	[more]
XP_038880166.1	1.3e-67	98.57	probable calcium-binding protein CML15 [Benincasa hispida]	[more]
KAE8652452.1	1.3e-67	98.57	hypothetical protein Csa_013816 [Cucumis sativus]	[more]
XP_022135250.1	6.2e-67	97.14	probable calcium-binding protein CML15 [Momordica charantia]	[more]

BLAST of Spg021959 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FZ75	1.1e-58	80.00	Probable calcium-binding protein CML15 OS=Arabidopsis thaliana OX=3702 GN=CML15 ...	[more]
Q9LI84	3.9e-56	79.29	Probable calcium-binding protein CML16 OS=Arabidopsis thaliana OX=3702 GN=CML16 ...	[more]
Q6L5F4	2.7e-49	71.22	Probable calcium-binding protein CML14 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q5ZD81	3.8e-43	63.64	Probable calcium-binding protein CML12 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9M8U1	1.2e-31	56.92	Probable calcium-binding protein CML18 OS=Arabidopsis thaliana OX=3702 GN=CML18 ...	[more]

BLAST of Spg021959 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DXV7	6.1e-68	98.57	Putative calcium-binding protein CML15 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A0A0LUS7	6.1e-68	98.57	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G015780 PE=4 SV=1	[more]
A0A1S3BX63	6.1e-68	98.57	probable calcium-binding protein CML15 OS=Cucumis melo OX=3656 GN=LOC103494094 P...	[more]
A0A6J1C0M6	3.0e-67	97.14	probable calcium-binding protein CML15 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1L5D2	6.7e-67	95.00	probable calcium-binding protein CML15 OS=Cucurbita maxima OX=3661 GN=LOC1114992...	[more]

BLAST of Spg021959 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G18530.1	7.9e-60	80.00	EF hand calcium-binding protein family	[more]
AT3G25600.1	2.8e-57	79.29	Calcium-binding EF-hand family protein	[more]
AT3G03000.1	8.2e-33	56.92	EF hand calcium-binding protein family	[more]
AT1G32250.1	1.7e-30	52.24	Calcium-binding EF-hand family protein	[more]
AT4G14640.1	5.9e-23	39.68	calmodulin 8	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 102..130 e-value: 8.4E-8 score: 41.9 coord: 27..55 e-value: 1.1 score: 17.6 coord: 66..94 e-value: 0.001 score: 28.4
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 66..127 e-value: 5.6E-15 score: 55.5 coord: 2..52 e-value: 1.7E-7 score: 31.5
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 1..22 score: 8.404312
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 62..97 score: 13.927603
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 98..133 score: 14.039183
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 23..58 score: 9.129593
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 66..128 e-value: 3.17423E-19 score: 73.7361
None	No IPR available	GENE3D	1.10.238.10		coord: 1..59 e-value: 3.1E-12 score: 48.8
None	No IPR available	GENE3D	1.10.238.10		coord: 60..137 e-value: 1.0E-23 score: 85.4
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 1..139
None	No IPR available	PANTHER	PTHR23050:SF351	CALCIUM-BINDING PROTEIN CML15-RELATED	coord: 1..139
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 111..123
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 75..87
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 36..48
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 2..128

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg021959.1	Spg021959.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050790	regulation of catalytic activity
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0030234	enzyme regulator activity