Spg021932 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg021932 vs. NCBI nr
Match: XP_023515400.1 (protein DETOXIFICATION 16-like isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 540.0 bits (1390), Expect = 1.5e-149
Identity = 292/364 (80.22%), Postives = 312/364 (85.71%), Query Frame = 0

Query: 24  MMYIQRGEVLNEVKRQLLLAGPLFSVGLLQYCLQIISLMFVGHLGELALAAASMATSFVS 83
           MM IQ GEVLNEVKRQLLLAGPLFS+GLLQY LQIISLMFVGHLGELALAAASMATSFVS
Sbjct: 1   MMDIQGGEVLNEVKRQLLLAGPLFSIGLLQYSLQIISLMFVGHLGELALAAASMATSFVS 60

Query: 84  VTGFSLLMGMASALDTLCGQSFGAKRYHMLGIHTQCAMFVLLIVSILIAIVSSNTKMIPI 143
           VTGFSLLMGMASALDTLCGQSFGAKRYHMLG+HTQCAMFVLL+VSI + I++SNTKMI I
Sbjct: 61  VTGFSLLMGMASALDTLCGQSFGAKRYHMLGLHTQCAMFVLLVVSIFLVIITSNTKMILI 120

Query: 144 ALHQDQEISEGAGLYARYMIPSVVAYGQLQCLVKFLQTQNIVFPMVLSSGVAALIHILLC 203
           ALHQDQ+IS+GAGLYARYMIPSVVAYGQLQCLVKFLQTQNIVFPMVLSSG+AALIHILLC
Sbjct: 121 ALHQDQDISKGAGLYARYMIPSVVAYGQLQCLVKFLQTQNIVFPMVLSSGIAALIHILLC 180

Query: 204 WILVSKFGLQIRGAALANSISYWINAFLTALYVKTSSCCSETWTGFTVDALHNVLAFLRI 263
           WILV +F L+IRG ALANSISYWIN  L ALYVK SSCCSETWTGFTVDALHNVLAFLRI
Sbjct: 181 WILVFEFELRIRGTALANSISYWINVCLIALYVKISSCCSETWTGFTVDALHNVLAFLRI 240

Query: 264 SIPSALMIC---------------------------------AAVRVSNELGAGHPQAAR 323
           S+PSALM+C                                  ++RVSNELGAG P+AAR
Sbjct: 241 SVPSALMLCLKVWTFEMVVILSGLLPNPKLETSVLSIWLVLLLSIRVSNELGAGRPRAAR 300

Query: 324 TAAYVALVMVVTEGVLVGFLMILLREIWGRVYSDEEEVVRYLATMMPTVAVSSFLDGVQS 355
            AAYVALVMVV EGVLVG LM+LLR++WGRVYS+EEEVVRYLATMMPTVAVSSF DGVQS
Sbjct: 301 MAAYVALVMVVVEGVLVGVLMVLLRDVWGRVYSEEEEVVRYLATMMPTVAVSSFFDGVQS 360

BLAST of Spg021932 vs. NCBI nr
Match: XP_023515398.1 (protein DETOXIFICATION 16-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 535.4 bits (1378), Expect = 3.7e-148
Identity = 292/377 (77.45%), Postives = 312/377 (82.76%), Query Frame = 0

Query: 24  MMYIQRGEVLNEVKRQLLLAGPLFSVGLLQYCLQIISLMFVGHLGELALAAASMATSFVS 83
           MM IQ GEVLNEVKRQLLLAGPLFS+GLLQY LQIISLMFVGHLGELALAAASMATSFVS
Sbjct: 1   MMDIQGGEVLNEVKRQLLLAGPLFSIGLLQYSLQIISLMFVGHLGELALAAASMATSFVS 60

Query: 84  VTGFSLLMGMASALDTLCGQSFGAKRYHMLGIHTQCAMFVLLIVSILIAIVSSNTKMIPI 143
           VTGFSLLMGMASALDTLCGQSFGAKRYHMLG+HTQCAMFVLL+VSI + I++SNTKMI I
Sbjct: 61  VTGFSLLMGMASALDTLCGQSFGAKRYHMLGLHTQCAMFVLLVVSIFLVIITSNTKMILI 120

Query: 144 ALHQDQEISEGAGLYARYMIPSVVAYGQLQCLVKFLQTQNIVFPMVLSSGVAALIHILLC 203
           ALHQDQ+IS+GAGLYARYMIPSVVAYGQLQCLVKFLQTQNIVFPMVLSSG+AALIHILLC
Sbjct: 121 ALHQDQDISKGAGLYARYMIPSVVAYGQLQCLVKFLQTQNIVFPMVLSSGIAALIHILLC 180

Query: 204 WILVSKFGLQIRGAALANSISYWINAFLTALYVKTSSCCSETWTGFTVDALHNVLAFLRI 263
           WILV +F L+IRG ALANSISYWIN  L ALYVK SSCCSETWTGFTVDALHNVLAFLRI
Sbjct: 181 WILVFEFELRIRGTALANSISYWINVCLIALYVKISSCCSETWTGFTVDALHNVLAFLRI 240

Query: 264 SIPSALMIC----------------------------------------------AAVRV 323
           S+PSALM+C                                               ++RV
Sbjct: 241 SVPSALMLCLKVWTFEMVVILSGLLPNPKLETSVLSICLNIFGLFWMISFGLSAAVSIRV 300

Query: 324 SNELGAGHPQAARTAAYVALVMVVTEGVLVGFLMILLREIWGRVYSDEEEVVRYLATMMP 355
           SNELGAG P+AAR AAYVALVMVV EGVLVG LM+LLR++WGRVYS+EEEVVRYLATMMP
Sbjct: 301 SNELGAGRPRAARMAAYVALVMVVVEGVLVGVLMVLLRDVWGRVYSEEEEVVRYLATMMP 360

BLAST of Spg021932 vs. NCBI nr
Match: XP_023515399.1 (protein DETOXIFICATION 16-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 535.4 bits (1378), Expect = 3.7e-148
Identity = 292/377 (77.45%), Postives = 312/377 (82.76%), Query Frame = 0

Query: 24  MMYIQRGEVLNEVKRQLLLAGPLFSVGLLQYCLQIISLMFVGHLGELALAAASMATSFVS 83
           MM IQ GEVLNEVKRQLLLAGPLFS+GLLQY LQIISLMFVGHLGELALAAASMATSFVS
Sbjct: 1   MMDIQGGEVLNEVKRQLLLAGPLFSIGLLQYSLQIISLMFVGHLGELALAAASMATSFVS 60

Query: 84  VTGFSLLMGMASALDTLCGQSFGAKRYHMLGIHTQCAMFVLLIVSILIAIVSSNTKMIPI 143
           VTGFSLLMGMASALDTLCGQSFGAKRYHMLG+HTQCAMFVLL+VSI + I++SNTKMI I
Sbjct: 61  VTGFSLLMGMASALDTLCGQSFGAKRYHMLGLHTQCAMFVLLVVSIFLVIITSNTKMILI 120

Query: 144 ALHQDQEISEGAGLYARYMIPSVVAYGQLQCLVKFLQTQNIVFPMVLSSGVAALIHILLC 203
           ALHQDQ+IS+GAGLYARYMIPSVVAYGQLQCLVKFLQTQNIVFPMVLSSG+AALIHILLC
Sbjct: 121 ALHQDQDISKGAGLYARYMIPSVVAYGQLQCLVKFLQTQNIVFPMVLSSGIAALIHILLC 180

Query: 204 WILVSKFGLQIRGAALANSISYWINAFLTALYVKTSSCCSETWTGFTVDALHNVLAFLRI 263
           WILV +F L+IRG ALANSISYWIN  L ALYVK SSCCSETWTGFTVDALHNVLAFLRI
Sbjct: 181 WILVFEFELRIRGTALANSISYWINVCLIALYVKISSCCSETWTGFTVDALHNVLAFLRI 240

Query: 264 SIPSALMIC----------------------------------------------AAVRV 323
           S+PSALM+C                                               ++RV
Sbjct: 241 SVPSALMLCLKVWTFEMVVILSGLLPNPKLETSVLSICLNIFGLFWMISFGLSAAVSIRV 300

Query: 324 SNELGAGHPQAARTAAYVALVMVVTEGVLVGFLMILLREIWGRVYSDEEEVVRYLATMMP 355
           SNELGAG P+AAR AAYVALVMVV EGVLVG LM+LLR++WGRVYS+EEEVVRYLATMMP
Sbjct: 301 SNELGAGRPRAARMAAYVALVMVVVEGVLVGVLMVLLRDVWGRVYSEEEEVVRYLATMMP 360

BLAST of Spg021932 vs. NCBI nr
Match: KAG6589623.1 (Protein DETOXIFICATION 16, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 526.2 bits (1354), Expect = 2.3e-145
Identity = 288/377 (76.39%), Postives = 308/377 (81.70%), Query Frame = 0

Query: 24  MMYIQRGEVLNEVKRQLLLAGPLFSVGLLQYCLQIISLMFVGHLGELALAAASMATSFVS 83
           MM IQ GEVL EVKRQLLLAGPLFS+GLLQY LQIISLMFVGHLGELALAAASMATSFVS
Sbjct: 1   MMDIQGGEVLIEVKRQLLLAGPLFSIGLLQYSLQIISLMFVGHLGELALAAASMATSFVS 60

Query: 84  VTGFSLLMGMASALDTLCGQSFGAKRYHMLGIHTQCAMFVLLIVSILIAIVSSNTKMIPI 143
           VTGFSLLMGMASALDTLCGQSFGAKRYHMLG+HTQCAMFVLL+VSI + I++SNTKMI I
Sbjct: 61  VTGFSLLMGMASALDTLCGQSFGAKRYHMLGLHTQCAMFVLLVVSIFLVIITSNTKMILI 120

Query: 144 ALHQDQEISEGAGLYARYMIPSVVAYGQLQCLVKFLQTQNIVFPMVLSSGVAALIHILLC 203
           ALHQDQ+IS+GAGLYARYMIPSVVAYGQLQCLVKFLQTQNIVFPMVLSSG+AALIHILLC
Sbjct: 121 ALHQDQDISKGAGLYARYMIPSVVAYGQLQCLVKFLQTQNIVFPMVLSSGIAALIHILLC 180

Query: 204 WILVSKFGLQIRGAALANSISYWINAFLTALYVKTSSCCSETWTGFTVDALHNVLAFLRI 263
           WILV +F L+IRG ALANSISYWIN  L ALYVK SSCCSETWTGFTVDALHNVL FLRI
Sbjct: 181 WILVFEFELRIRGTALANSISYWINVCLIALYVKISSCCSETWTGFTVDALHNVLDFLRI 240

Query: 264 SIPSALMIC----------------------------------------------AAVRV 323
           S+PSALM+C                                               ++RV
Sbjct: 241 SVPSALMLCLKVWTFEMMVILSGLLPNPKLETSVLSICLNIFGLFWMISFGLSAAVSIRV 300

Query: 324 SNELGAGHPQAARTAAYVALVMVVTEGVLVGFLMILLREIWGRVYSDEEEVVRYLATMMP 355
           SNELGAG P+AAR AAYVALVMVV EG LVG LM+LLR++WGRVYS+EEEVVRYLA MMP
Sbjct: 301 SNELGAGRPRAARMAAYVALVMVVVEGALVGVLMVLLRDVWGRVYSEEEEVVRYLAAMMP 360

BLAST of Spg021932 vs. NCBI nr
Match: XP_023925834.1 (protein DETOXIFICATION 16-like, partial [Quercus suber])

HSP 1 Score: 418.3 bits (1074), Expect = 6.6e-113
Identity = 225/377 (59.68%), Postives = 275/377 (72.94%), Query Frame = 0

Query: 24  MMYIQRGEVLNEVKRQLLLAGPLFSVGLLQYCLQIISLMFVGHLGELALAAASMATSFVS 83
           M  I+R E++ EVK+QL LAGPLFS+GLL Y +Q IS+MFVGHLGELAL+ AS+ATSF S
Sbjct: 1   MRTIKRKEIVEEVKKQLWLAGPLFSIGLLHYSIQAISVMFVGHLGELALSGASLATSFAS 60

Query: 84  VTGFSLLMGMASALDTLCGQSFGAKRYHMLGIHTQCAMFVLLIVSILIAIVSSNTKMIPI 143
           VTGF+LLMG+  ALD LCGQSFGA ++HMLGIHTQ AMF+LL+ S  ++++ ++TK I I
Sbjct: 61  VTGFNLLMGLGCALDALCGQSFGAMQFHMLGIHTQRAMFILLLTSAFLSVIWAHTKPILI 120

Query: 144 ALHQDQEISEGAGLYARYMIPSVVAYGQLQCLVKFLQTQNIVFPMVLSSGVAALIHILLC 203
            +HQ+ EIS+ AG+YA +MIP + AYG LQCL+KFLQTQNIVFPMVLSSG++ALIHI +C
Sbjct: 121 VMHQNSEISKEAGVYATFMIPCLFAYGLLQCLIKFLQTQNIVFPMVLSSGISALIHIPVC 180

Query: 204 WILVSKFGLQIRGAALANSISYWINAFLTALYVKTSSCCSETWTGFTVDALHNVLAFLRI 263
           WILV K GL  +GAA+ANSISYWIN  L ALY+K SS C ETWTGF+ +ALHN+  FL+I
Sbjct: 181 WILVFKSGLGSKGAAIANSISYWINVLLMALYIKFSSSCKETWTGFSKEALHNLAEFLKI 240

Query: 264 SIPSALMIC----------------------------------------------AAVRV 323
           +IPSALM+C                                               ++RV
Sbjct: 241 AIPSALMLCLKVWSFELIIILSGLLPNPQLETSVLSICQNTFGLLWMIPFGLGAAVSIRV 300

Query: 324 SNELGAGHPQAARTAAYVALVMVVTEGVLVGFLMILLREIWGRVYSDEEEVVRYLATMMP 355
           SNELGAG P+AAR A  V LVM +TEG+LVG LMILLR IWG VYS E+EVV+Y+A MMP
Sbjct: 301 SNELGAGKPKAARLAVRVVLVMAITEGMLVGLLMILLRNIWGHVYSHEQEVVKYVAVMMP 360

BLAST of Spg021932 vs. ExPASy Swiss-Prot
Match: Q9FHB6 (Protein DETOXIFICATION 16 OS=Arabidopsis thaliana OX=3702 GN=DTX16 PE=2 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 2.5e-94
Identity = 189/373 (50.67%), Postives = 250/373 (67.02%), Query Frame = 0

Query: 28  QRGEVLNEVKRQLLLAGPLFSVGLLQYCLQIISLMFVGHLGELALAAASMATSFVSVTGF 87
           ++  V  EVK+QL L+GPL +V LLQ+CLQ+IS+MFVGHLG L L+AAS+ATSF SVTGF
Sbjct: 19  EKSSVKEEVKKQLWLSGPLIAVSLLQFCLQVISVMFVGHLGSLPLSAASIATSFASVTGF 78

Query: 88  SLLMGMASALDTLCGQSFGAKRYHMLGIHTQCAMFVLLIVSILIAIVSSNTKMIPIALHQ 147
           S LMG ASALDTLCGQ++GAK+Y MLGI  Q AMFVL + SI ++I+ +NT+ + +   Q
Sbjct: 79  SFLMGTASALDTLCGQAYGAKKYGMLGIQMQRAMFVLTLASIPLSIIWANTEHLLVFFGQ 138

Query: 148 DQEISEGAGLYARYMIPSVVAYGQLQCLVKFLQTQNIVFPMVLSSGVAALIHILLCWILV 207
           ++ I+  AG YA++MIPS+ AYG LQC  +FLQ QN VFP+V  SGV   +H+LLCW+LV
Sbjct: 139 NKSIATLAGSYAKFMIPSIFAYGLLQCFNRFLQAQNNVFPVVFCSGVTTSLHVLLCWVLV 198

Query: 208 SKFGLQIRGAALANSISYWINAFLTALYVKTSSCCSETWTGFTVDALHNVLAFLRISIPS 267
            K GL  +GAALANSISYW+N  L   YVK S  CS TWTGF+ +AL ++L FLR+++PS
Sbjct: 199 FKSGLGFQGAALANSISYWLNVVLLFCYVKFSPSCSLTWTGFSKEALRDILPFLRLAVPS 258

Query: 268 ALMIC----------------------------------------------AAVRVSNEL 327
           ALM+C                                              A+ R+SNEL
Sbjct: 259 ALMVCLEMWSFELLVLLSGLLPNPVLETSVLSICLNTSGTMWMIPFGLSGAASTRISNEL 318

Query: 328 GAGHPQAARTAAYVALVMVVTEGVLVGFLMILLREIWGRVYSDEEEVVRYLATMMPTVAV 355
           GAG+P+ A+ A  V + + V E +++G ++IL+R IWG  YS E EVV Y+A+MMP +A+
Sbjct: 319 GAGNPKVAKLAVRVVICIAVAESIVIGSVLILIRNIWGLAYSSELEVVSYVASMMPILAL 378

BLAST of Spg021932 vs. ExPASy Swiss-Prot
Match: F4IHU9 (Protein DETOXIFICATION 15 OS=Arabidopsis thaliana OX=3702 GN=DTX15 PE=3 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 4.1e-89
Identity = 179/363 (49.31%), Postives = 244/363 (67.22%), Query Frame = 0

Query: 32  VLNEVKRQLLLAGPLFSVGLLQYCLQIISLMFVGHLGELALAAASMATSFVSVTGFSLLM 91
           V  EV++QLLL+GPL +V LLQ+CLQIIS+MFVGHLG L L+AAS+ATSF SVTGF+ LM
Sbjct: 24  VKEEVEKQLLLSGPLIAVSLLQFCLQIISVMFVGHLGSLPLSAASIATSFASVTGFTFLM 83

Query: 92  GMASALDTLCGQSFGAKRYHMLGIHTQCAMFVLLIVSILIAIVSSNTKMIPIALHQDQEI 151
           G ASA+DT+CGQS+GAK Y MLGI  Q AM VL ++S+ ++IV +NT+   +   QD+ I
Sbjct: 84  GTASAMDTVCGQSYGAKMYGMLGIQMQRAMLVLTLLSVPLSIVWANTEHFLVFFGQDKSI 143

Query: 152 SEGAGLYARYMIPSVVAYGQLQCLVKFLQTQNIVFPMVLSSGVAALIHILLCWILVSKFG 211
           +  +G YAR+MIPS+ AYG LQCL +FLQ QN V P+V+ SGV   +H+++CW+LV K G
Sbjct: 144 AHLSGSYARFMIPSIFAYGLLQCLNRFLQAQNNVIPVVICSGVTTSLHVIICWVLVLKSG 203

Query: 212 LQIRGAALANSISYWINAFLTALYVKTSSCCSETWTGFTVDALHNVLAFLRISIPSALMI 271
           L  RGAA+AN+ISYW+N  L + YVK S  CS TWTGF+ +A  +++ F+++ IPSA M+
Sbjct: 204 LGFRGAAVANAISYWLNVILLSCYVKFSPSCSLTWTGFSKEARRDIIPFMKLVIPSAFMV 263

Query: 272 C----------------------------------------AAVRVSNELGAGHPQAART 331
           C                                        A+ RVSNELG+G+P+ A+ 
Sbjct: 264 CSLEMWSFELLVLSSGLLPNPVLETSCPRTVWMIPFGLSGAASTRVSNELGSGNPKGAKL 323

Query: 332 AAYVALVMVVTEGVLVGFLMILLREIWGRVYSDEEEVVRYLATMMPTVAVSSFLDGVQSV 355
           A  V L   + E +LVG ++IL+R+IWG  YS + EVV ++A+M+P +A+   LD  Q+V
Sbjct: 324 AVRVVLSFSIVESILVGTVLILIRKIWGFAYSSDPEVVSHVASMLPILALGHSLDSFQTV 383

BLAST of Spg021932 vs. ExPASy Swiss-Prot
Match: Q9C9U1 (Protein DETOXIFICATION 17 OS=Arabidopsis thaliana OX=3702 GN=DTX17 PE=2 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 3.8e-87
Identity = 177/369 (47.97%), Postives = 241/369 (65.31%), Query Frame = 0

Query: 32  VLNEVKRQLLLAGPLFSVGLLQYCLQIISLMFVGHLGELALAAASMATSFVSVTGFSLLM 91
           V  EVK+QL L+ PL  V LLQY LQ+IS+MFVGHLG L L+AAS+ATSF SVTGF+ L+
Sbjct: 21  VKEEVKKQLWLSAPLIGVSLLQYSLQVISVMFVGHLGSLPLSAASIATSFASVTGFTFLL 80

Query: 92  GMASALDTLCGQSFGAKRYHMLGIHTQCAMFVLLIVSILIAIVSSNTKMIPIALHQDQEI 151
           G ASAL+TLCGQ++GAK Y  LGI  Q AMFVLLI+S+ ++I+ +NT+ I + +HQD+ I
Sbjct: 81  GTASALETLCGQAYGAKLYGKLGIQMQRAMFVLLILSVPLSIIWANTEQILVLVHQDKSI 140

Query: 152 SEGAGLYARYMIPSVVAYGQLQCLVKFLQTQNIVFPMVLSSGVAALIHILLCWILVSKFG 211
           +  AG YA+YMIPS+ AYG LQC+ +FLQ QN VFP+ + SG+   +H+LLCW+ V K G
Sbjct: 141 ASVAGSYAKYMIPSLFAYGLLQCINRFLQAQNNVFPVFVCSGITTCLHLLLCWLFVLKTG 200

Query: 212 LQIRGAALANSISYWINAFLTALYVKTSSCCSETWTGFTVDALHNVLAFLRISIPSALMI 271
           L  RGAALA S+SYW N  L + YVK S  CS +WTGF+ +A   +  F +I+ PSA+M+
Sbjct: 201 LGYRGAALAISVSYWFNVILLSCYVKFSPSCSHSWTGFSKEAFQELYDFSKIAFPSAVMV 260

Query: 272 C----------------------------------------------AAVRVSNELGAGH 331
           C                                              A++RVSNELGAG+
Sbjct: 261 CLELWSFELLVLASGLLPNPVLETSVLSICLNTSLTIWQISVGLGGAASIRVSNELGAGN 320

Query: 332 PQAARTAAYVALVMVVTEGVLVGFLMILLREIWGRVYSDEEEVVRYLATMMPTVAVSSFL 355
           PQ A+ A YV + + V EG++V  +++ +R+I G  +S + +++ Y A+M+P VA  +FL
Sbjct: 321 PQVAKLAVYVIVGIAVAEGIVVVTVLLSIRKILGHAFSSDPKIIAYAASMIPIVACGNFL 380

BLAST of Spg021932 vs. ExPASy Swiss-Prot
Match: Q9C994 (Protein DETOXIFICATION 14 OS=Arabidopsis thaliana OX=3702 GN=DTX14 PE=1 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 9.4e-62
Identity = 140/368 (38.04%), Postives = 212/368 (57.61%), Query Frame = 0

Query: 33  LNEVKRQLLLAGPLFSVGLLQYCLQIISLMFVGHLGELALAAASMATSFVSVTGFSLLMG 92
           L E K+   +AGP+ +V    Y LQ+IS+M VGHLGEL L++ ++A SF SVTGFS++ G
Sbjct: 24  LRETKKLSYIAGPMIAVNSSMYVLQVISIMMVGHLGELFLSSTAIAVSFCSVTGFSVVFG 83

Query: 93  MASALDTLCGQSFGAKRYHMLGIHTQCAMFVLLIVSILIAIVSSNTKMIPIALHQDQEIS 152
           +ASAL+TLCGQ+ GAK+Y  LG+HT   +  L +V I ++++ +    I   + QD  ++
Sbjct: 84  LASALETLCGQANGAKQYEKLGVHTYTGIVSLFLVCIPLSLLWTYIGDILSLIGQDAMVA 143

Query: 153 EGAGLYARYMIPSVVAYGQLQCLVKFLQTQNIVFPMVLSSGVAALIHILLCWILVSKFGL 212
           + AG +A ++IP++  Y  LQ LV+F Q Q+++ P+V+SS  +  IHI+LCW LV KFGL
Sbjct: 144 QEAGKFATWLIPALFGYATLQPLVRFFQAQSLILPLVMSSVSSLCIHIVLCWSLVFKFGL 203

Query: 213 QIRGAALANSISYWINAFLTALYVKTSSCCSETWTGFTVDALHNVLAFLRISIPSALMIC 272
              GAA+A  +SYW+N  +  LY+  SS CS++    ++     +  F R  IPSA MIC
Sbjct: 204 GSLGAAIAIGVSYWLNVTVLGLYMTFSSSCSKSRATISMSLFEGMGEFFRFGIPSASMIC 263

Query: 273 ----------------------------------------------AAVRVSNELGAGHP 332
                                                         A+ RV+NELGAG+P
Sbjct: 264 LEWWSFEFLVLLSGILPNPKLEASVLSVCLSTQSSLYQIPESLGAAASTRVANELGAGNP 323

Query: 333 QAARTAAYVALVMVVTEGVLVGFLMILLREIWGRVYSDEEEVVRYLATMMPTVAVSSFLD 355
           + AR A Y A+V+   E ++VG ++   R ++G ++S E EVV Y+ +M P +++S   D
Sbjct: 324 KQARMAVYTAMVITGVESIMVGAIVFGARNVFGYLFSSETEVVDYVKSMAPLLSLSVIFD 383

BLAST of Spg021932 vs. ExPASy Swiss-Prot
Match: Q8VYL8 (Protein DETOXIFICATION 10 OS=Arabidopsis thaliana OX=3702 GN=DTX10 PE=2 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 3.6e-61
Identity = 137/381 (35.96%), Postives = 216/381 (56.69%), Query Frame = 0

Query: 20  EKRDMMYIQRGEVLNEVKRQLLLAGPLFSVGLLQYCLQIISLMFVGHLGELALAAASMAT 79
           EK     +Q G    E+KR +  A P+ +V ++Q+ +QIIS++ VGHLG L+LA+AS A 
Sbjct: 16  EKVTWRDLQDGSFTAELKRLICFAAPMAAVVIIQFMIQIISMVMVGHLGRLSLASASFAV 75

Query: 80  SFVSVTGFSLLMGMASALDTLCGQSFGAKRYHMLGIHTQCAMFVLLIVSILIAIVSSNTK 139
           SF +VTGFS ++G++ ALDTL GQ++GAK Y  LG+    AMF L +V + ++++  N  
Sbjct: 76  SFCNVTGFSFIIGLSCALDTLSGQAYGAKLYRKLGVQAYTAMFCLTLVCLPLSLLWFNMG 135

Query: 140 MIPIALHQDQEISEGAGLYARYMIPSVVAYGQLQCLVKFLQTQNIVFPMVLSSGVAALIH 199
            + + L QD  I+  AG YA ++IP + AY  LQ L+++ + Q+++ P++++S V   IH
Sbjct: 136 KLIVILGQDPAIAHEAGRYAAWLIPGLFAYAVLQPLIRYFKNQSLITPLLVTSSVVFCIH 195

Query: 200 ILLCWILVSKFGLQIRGAALANSISYWINAFLTALYVKTSSCCSETWTGFTVDALHNVLA 259
           + LCW+LV K GL   G ALA S+SYW+ A     ++  SS CSET    T++    V  
Sbjct: 196 VPLCWLLVYKSGLGHIGGALALSLSYWLYAIFLGSFMYYSSACSETRAPLTMEIFEGVRE 255

Query: 260 FLRISIPSALMIC----------------------------------------------A 319
           F++ ++PSA M+C                                              A
Sbjct: 256 FIKYALPSAAMLCLEWWSYELIILLSGLLPNPQLETSVLSICFETLSITYSIPLAIAAAA 315

Query: 320 AVRVSNELGAGHPQAARTAAYVALVMVVTEGVLVGFLMILLREIWGRVYSDEEEVVRYLA 355
           + R+SNELGAG+ +AA    Y A+ + V + ++V   ++  R ++G V+S +++ + Y+A
Sbjct: 316 STRISNELGAGNSRAAHIVVYAAMSLAVMDALMVSMSLLAGRHVFGHVFSSDKKTIEYVA 375

BLAST of Spg021932 vs. ExPASy TrEMBL
Match: A0A5B7BMX3 (Protein DETOXIFICATION (Fragment) OS=Davidia involucrata OX=16924 GN=Din_038917 PE=3 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 4.6e-112
Identity = 226/385 (58.70%), Postives = 275/385 (71.43%), Query Frame = 0

Query: 16  GFNDEKRDMMYIQRGEVLNEVKRQLLLAGPLFSVGLLQYCLQIISLMFVGHLGELALAAA 75
           GF  E+ +   I+R E++ EVK+Q+ LAGPL  V LLQ+CLQ+IS+MFVGHLGELAL+ A
Sbjct: 42  GFGRERNNENGIERTEIVEEVKKQIWLAGPLICVSLLQFCLQLISVMFVGHLGELALSGA 101

Query: 76  SMATSFVSVTGFSLLMGMASALDTLCGQSFGAKRYHMLGIHTQCAMFVLLIVSILIAIVS 135
           SMATSF SVTGFS+LMGMASALDT CGQS+GAK+YHMLGIH Q AMF+L +VSI  AI+ 
Sbjct: 102 SMATSFASVTGFSMLMGMASALDTFCGQSYGAKQYHMLGIHMQRAMFILTLVSIPFAIIW 161

Query: 136 SNTKMIPIALHQDQEISEGAGLYARYMIPSVVAYGQLQCLVKFLQTQNIVFPMVLSSGVA 195
           +NT  I  AL QD +IS  AGLYAR+MIPS+ AYG LQCL++FLQTQN VFPM+LSSG+ 
Sbjct: 162 ANTGPILKALGQDPDISTEAGLYARFMIPSLFAYGLLQCLIRFLQTQNNVFPMMLSSGIT 221

Query: 196 ALIHILLCWILVSKFGLQIRGAALANSISYWINAFLTALYVKTSSCCSETWTGFTVDALH 255
            L+HIL+CWILV K GL  RGAALANSISYWIN  L  LYVK S  CS+TWTG + +ALH
Sbjct: 222 TLLHILVCWILVFKSGLGSRGAALANSISYWINVVLLVLYVKFSPSCSKTWTGLSKEALH 281

Query: 256 NVLAFLRISIPSALMIC------------------------------------------- 315
           N+L FL+++IPSA+M+C                                           
Sbjct: 282 NILTFLKLAIPSAVMVCLEMWSFEMMVLLSGLLPNPELETSVLSISLNTASTVWMISFGL 341

Query: 316 ---AAVRVSNELGAGHPQAARTAAYVALVMVVTEGVLVGFLMILLREIWGRVYSDEEEVV 355
              A+ RVSNELGAGHP+ AR A  V LV  +T G+LVG ++IL+R IWG  YS+E EVV
Sbjct: 342 SGAASTRVSNELGAGHPKTARLAVCVVLVTAITAGILVGLVLILIRNIWGYAYSNEIEVV 401

BLAST of Spg021932 vs. ExPASy TrEMBL
Match: A0A067F632 (Protein DETOXIFICATION OS=Citrus sinensis OX=2711 GN=CISIN_1g010345mg PE=3 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 6.0e-112
Identity = 219/373 (58.71%), Postives = 278/373 (74.53%), Query Frame = 0

Query: 28  QRGEVLNEVKRQLLLAGPLFSVGLLQYCLQIISLMFVGHLGELALAAASMATSFVSVTGF 87
           ++ +++ EVKRQL L+GPL SV LLQ+CLQ+IS+MFVGHLGEL L+ ASMATSF +VTGF
Sbjct: 31  EKKKIIEEVKRQLWLSGPLISVSLLQFCLQMISVMFVGHLGELPLSGASMATSFATVTGF 90

Query: 88  SLLMGMASALDTLCGQSFGAKRYHMLGIHTQCAMFVLLIVSILIAIVSSNTKMIPIALHQ 147
           S L+G++SAL+TLCGQS+GAK+YHMLGIH Q  MFVLL++S+ +A++ +NT+ + +A HQ
Sbjct: 91  SFLVGLSSALETLCGQSYGAKQYHMLGIHMQRGMFVLLLISVPLALIWANTESLLVAAHQ 150

Query: 148 DQEISEGAGLYARYMIPSVVAYGQLQCLVKFLQTQNIVFPMVLSSGVAALIHILLCWILV 207
           D EIS+ AGLYARYMIPS+ AY  LQCLV+FLQTQNIVFPMVLSSG+ AL+HIL+CWILV
Sbjct: 151 DSEISKEAGLYARYMIPSIFAYAILQCLVRFLQTQNIVFPMVLSSGITALLHILVCWILV 210

Query: 208 SKFGLQIRGAALANSISYWINAFLTALYVKTSSCCSETWTGFTVDALHNVLAFLRISIPS 267
            K GL  RGAALANSISYWIN  L A+YVK SS C++TWTGF+ +AL+NV  F+R++IPS
Sbjct: 211 FKSGLGSRGAALANSISYWINVLLLAIYVKFSSSCAKTWTGFSKEALNNVFNFVRLAIPS 270

Query: 268 ALMIC----------------------------------------------AAVRVSNEL 327
           A+M+C                                              A+ RVSNEL
Sbjct: 271 AVMVCLEMWSFEMMVLLSGLLPNPALETSVLSISLNTASTVWMIPFGLSGAASTRVSNEL 330

Query: 328 GAGHPQAARTAAYVALVMVVTEGVLVGFLMILLREIWGRVYSDEEEVVRYLATMMPTVAV 355
           GAGHP+AAR A  V LVM VTEG+LVG ++IL+R +WG  YS+E EVV+Y+A+M+P +A 
Sbjct: 331 GAGHPEAARLAVCVVLVMAVTEGILVGSVLILIRNVWGYAYSNEIEVVKYVASMLPILAA 390

BLAST of Spg021932 vs. ExPASy TrEMBL
Match: A0A067F671 (Protein DETOXIFICATION OS=Citrus sinensis OX=2711 GN=CISIN_1g010345mg PE=3 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 6.0e-112
Identity = 219/373 (58.71%), Postives = 278/373 (74.53%), Query Frame = 0

Query: 28  QRGEVLNEVKRQLLLAGPLFSVGLLQYCLQIISLMFVGHLGELALAAASMATSFVSVTGF 87
           ++ +++ EVKRQL L+GPL SV LLQ+CLQ+IS+MFVGHLGEL L+ ASMATSF +VTGF
Sbjct: 31  EKKKIIEEVKRQLWLSGPLISVSLLQFCLQMISVMFVGHLGELPLSGASMATSFATVTGF 90

Query: 88  SLLMGMASALDTLCGQSFGAKRYHMLGIHTQCAMFVLLIVSILIAIVSSNTKMIPIALHQ 147
           S L+G++SAL+TLCGQS+GAK+YHMLGIH Q  MFVLL++S+ +A++ +NT+ + +A HQ
Sbjct: 91  SFLVGLSSALETLCGQSYGAKQYHMLGIHMQRGMFVLLLISVPLALIWANTESLLVAAHQ 150

Query: 148 DQEISEGAGLYARYMIPSVVAYGQLQCLVKFLQTQNIVFPMVLSSGVAALIHILLCWILV 207
           D EIS+ AGLYARYMIPS+ AY  LQCLV+FLQTQNIVFPMVLSSG+ AL+HIL+CWILV
Sbjct: 151 DSEISKEAGLYARYMIPSIFAYAILQCLVRFLQTQNIVFPMVLSSGITALLHILVCWILV 210

Query: 208 SKFGLQIRGAALANSISYWINAFLTALYVKTSSCCSETWTGFTVDALHNVLAFLRISIPS 267
            K GL  RGAALANSISYWIN  L A+YVK SS C++TWTGF+ +AL+NV  F+R++IPS
Sbjct: 211 FKSGLGSRGAALANSISYWINVLLLAIYVKFSSSCAKTWTGFSKEALNNVFNFVRLAIPS 270

Query: 268 ALMIC----------------------------------------------AAVRVSNEL 327
           A+M+C                                              A+ RVSNEL
Sbjct: 271 AVMVCLEMWSFEMMVLLSGLLPNPALETSVLSISLNTASTVWMIPFGLSGAASTRVSNEL 330

Query: 328 GAGHPQAARTAAYVALVMVVTEGVLVGFLMILLREIWGRVYSDEEEVVRYLATMMPTVAV 355
           GAGHP+AAR A  V LVM VTEG+LVG ++IL+R +WG  YS+E EVV+Y+A+M+P +A 
Sbjct: 331 GAGHPEAARLAVCVVLVMAVTEGILVGSVLILIRNVWGYAYSNEIEVVKYVASMLPILAA 390

BLAST of Spg021932 vs. ExPASy TrEMBL
Match: A0A067F9N3 (Protein DETOXIFICATION OS=Citrus sinensis OX=2711 GN=CISIN_1g010345mg PE=3 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 6.0e-112
Identity = 219/373 (58.71%), Postives = 278/373 (74.53%), Query Frame = 0

Query: 28  QRGEVLNEVKRQLLLAGPLFSVGLLQYCLQIISLMFVGHLGELALAAASMATSFVSVTGF 87
           ++ +++ EVKRQL L+GPL SV LLQ+CLQ+IS+MFVGHLGEL L+ ASMATSF +VTGF
Sbjct: 31  EKKKIIEEVKRQLWLSGPLISVSLLQFCLQMISVMFVGHLGELPLSGASMATSFATVTGF 90

Query: 88  SLLMGMASALDTLCGQSFGAKRYHMLGIHTQCAMFVLLIVSILIAIVSSNTKMIPIALHQ 147
           S L+G++SAL+TLCGQS+GAK+YHMLGIH Q  MFVLL++S+ +A++ +NT+ + +A HQ
Sbjct: 91  SFLVGLSSALETLCGQSYGAKQYHMLGIHMQRGMFVLLLISVPLALIWANTESLLVAAHQ 150

Query: 148 DQEISEGAGLYARYMIPSVVAYGQLQCLVKFLQTQNIVFPMVLSSGVAALIHILLCWILV 207
           D EIS+ AGLYARYMIPS+ AY  LQCLV+FLQTQNIVFPMVLSSG+ AL+HIL+CWILV
Sbjct: 151 DSEISKEAGLYARYMIPSIFAYAILQCLVRFLQTQNIVFPMVLSSGITALLHILVCWILV 210

Query: 208 SKFGLQIRGAALANSISYWINAFLTALYVKTSSCCSETWTGFTVDALHNVLAFLRISIPS 267
            K GL  RGAALANSISYWIN  L A+YVK SS C++TWTGF+ +AL+NV  F+R++IPS
Sbjct: 211 FKSGLGSRGAALANSISYWINVLLLAIYVKFSSSCAKTWTGFSKEALNNVFNFVRLAIPS 270

Query: 268 ALMIC----------------------------------------------AAVRVSNEL 327
           A+M+C                                              A+ RVSNEL
Sbjct: 271 AVMVCLEMWSFEMMVLLSGLLPNPALETSVLSISLNTASTVWMIPFGLSGAASTRVSNEL 330

Query: 328 GAGHPQAARTAAYVALVMVVTEGVLVGFLMILLREIWGRVYSDEEEVVRYLATMMPTVAV 355
           GAGHP+AAR A  V LVM VTEG+LVG ++IL+R +WG  YS+E EVV+Y+A+M+P +A 
Sbjct: 331 GAGHPEAARLAVCVVLVMAVTEGILVGSVLILIRNVWGYAYSNEIEVVKYVASMLPILAA 390

BLAST of Spg021932 vs. ExPASy TrEMBL
Match: A0A2R6P351 (Protein DETOXIFICATION OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc21826 PE=3 SV=1)

HSP 1 Score: 412.9 bits (1060), Expect = 1.3e-111
Identity = 220/369 (59.62%), Postives = 273/369 (73.98%), Query Frame = 0

Query: 32  VLNEVKRQLLLAGPLFSVGLLQYCLQIISLMFVGHLGELALAAASMATSFVSVTGFSLLM 91
           ++ E+K+QL LAGPL  V LLQ+CLQ+IS+MFVGHLGEL+L+ ASMATSF SVTGFS+L+
Sbjct: 26  IVEELKKQLWLAGPLICVNLLQFCLQLISVMFVGHLGELSLSGASMATSFASVTGFSMLV 85

Query: 92  GMASALDTLCGQSFGAKRYHMLGIHTQCAMFVLLIVSILIAIVSSNTKMIPIALHQDQEI 151
           GMASALDTLCGQS+GAK YHMLGIH Q AMFVLL+VSI ++++ +NT  I +A  QD EI
Sbjct: 86  GMASALDTLCGQSYGAKEYHMLGIHMQRAMFVLLLVSIPLSVIWANTGPILVAFGQDSEI 145

Query: 152 SEGAGLYARYMIPSVVAYGQLQCLVKFLQTQNIVFPMVLSSGVAALIHILLCWILVSKFG 211
           SE A LYARYMIPS+ AYG LQCLVKFLQTQNIVFPM++SSG+  L+H+L+CW+LV K G
Sbjct: 146 SEVAQLYARYMIPSIFAYGLLQCLVKFLQTQNIVFPMMISSGITTLVHLLMCWVLVFKSG 205

Query: 212 LQIRGAALANSISYWINAFLTALYVKTSSCCSETWTGFTVDALHNVLAFLRISIPSALMI 271
           L +RGAALANSISYW N  L ALYVK SS C ++WTGF+ +AL N+L FL+++IPSA+M+
Sbjct: 206 LGVRGAALANSISYWTNVILLALYVKFSSSCVKSWTGFSKEALQNILFFLKLAIPSAVMV 265

Query: 272 C----------------------------------------------AAVRVSNELGAGH 331
           C                                               + RVSNELGAGH
Sbjct: 266 CLEMWSFEMMVLLSGLLPNPQLETSVLSVCLNTASTVWMIPFGLSNAVSTRVSNELGAGH 325

Query: 332 PQAARTAAYVALVMVVTEGVLVGFLMILLREIWGRVYSDEEEVVRYLATMMPTVAVSSFL 355
           PQAAR A  V LV+ VTEG+LVG ++IL+R IWG  YS+E EVVR++A+MMP +A+S+F+
Sbjct: 326 PQAARLAVNVVLVLAVTEGILVGAILILIRNIWGYAYSNEVEVVRFVASMMPILAISNFM 385

BLAST of Spg021932 vs. TAIR 10
Match: AT5G52450.1 (MATE efflux family protein )

HSP 1 Score: 347.1 bits (889), Expect = 1.7e-95
Identity = 189/373 (50.67%), Postives = 250/373 (67.02%), Query Frame = 0

Query: 28  QRGEVLNEVKRQLLLAGPLFSVGLLQYCLQIISLMFVGHLGELALAAASMATSFVSVTGF 87
           ++  V  EVK+QL L+GPL +V LLQ+CLQ+IS+MFVGHLG L L+AAS+ATSF SVTGF
Sbjct: 19  EKSSVKEEVKKQLWLSGPLIAVSLLQFCLQVISVMFVGHLGSLPLSAASIATSFASVTGF 78

Query: 88  SLLMGMASALDTLCGQSFGAKRYHMLGIHTQCAMFVLLIVSILIAIVSSNTKMIPIALHQ 147
           S LMG ASALDTLCGQ++GAK+Y MLGI  Q AMFVL + SI ++I+ +NT+ + +   Q
Sbjct: 79  SFLMGTASALDTLCGQAYGAKKYGMLGIQMQRAMFVLTLASIPLSIIWANTEHLLVFFGQ 138

Query: 148 DQEISEGAGLYARYMIPSVVAYGQLQCLVKFLQTQNIVFPMVLSSGVAALIHILLCWILV 207
           ++ I+  AG YA++MIPS+ AYG LQC  +FLQ QN VFP+V  SGV   +H+LLCW+LV
Sbjct: 139 NKSIATLAGSYAKFMIPSIFAYGLLQCFNRFLQAQNNVFPVVFCSGVTTSLHVLLCWVLV 198

Query: 208 SKFGLQIRGAALANSISYWINAFLTALYVKTSSCCSETWTGFTVDALHNVLAFLRISIPS 267
            K GL  +GAALANSISYW+N  L   YVK S  CS TWTGF+ +AL ++L FLR+++PS
Sbjct: 199 FKSGLGFQGAALANSISYWLNVVLLFCYVKFSPSCSLTWTGFSKEALRDILPFLRLAVPS 258

Query: 268 ALMIC----------------------------------------------AAVRVSNEL 327
           ALM+C                                              A+ R+SNEL
Sbjct: 259 ALMVCLEMWSFELLVLLSGLLPNPVLETSVLSICLNTSGTMWMIPFGLSGAASTRISNEL 318

Query: 328 GAGHPQAARTAAYVALVMVVTEGVLVGFLMILLREIWGRVYSDEEEVVRYLATMMPTVAV 355
           GAG+P+ A+ A  V + + V E +++G ++IL+R IWG  YS E EVV Y+A+MMP +A+
Sbjct: 319 GAGNPKVAKLAVRVVICIAVAESIVIGSVLILIRNIWGLAYSSELEVVSYVASMMPILAL 378

BLAST of Spg021932 vs. TAIR 10
Match: AT2G34360.1 (MATE efflux family protein )

HSP 1 Score: 329.7 bits (844), Expect = 2.9e-90
Identity = 179/363 (49.31%), Postives = 244/363 (67.22%), Query Frame = 0

Query: 32  VLNEVKRQLLLAGPLFSVGLLQYCLQIISLMFVGHLGELALAAASMATSFVSVTGFSLLM 91
           V  EV++QLLL+GPL +V LLQ+CLQIIS+MFVGHLG L L+AAS+ATSF SVTGF+ LM
Sbjct: 24  VKEEVEKQLLLSGPLIAVSLLQFCLQIISVMFVGHLGSLPLSAASIATSFASVTGFTFLM 83

Query: 92  GMASALDTLCGQSFGAKRYHMLGIHTQCAMFVLLIVSILIAIVSSNTKMIPIALHQDQEI 151
           G ASA+DT+CGQS+GAK Y MLGI  Q AM VL ++S+ ++IV +NT+   +   QD+ I
Sbjct: 84  GTASAMDTVCGQSYGAKMYGMLGIQMQRAMLVLTLLSVPLSIVWANTEHFLVFFGQDKSI 143

Query: 152 SEGAGLYARYMIPSVVAYGQLQCLVKFLQTQNIVFPMVLSSGVAALIHILLCWILVSKFG 211
           +  +G YAR+MIPS+ AYG LQCL +FLQ QN V P+V+ SGV   +H+++CW+LV K G
Sbjct: 144 AHLSGSYARFMIPSIFAYGLLQCLNRFLQAQNNVIPVVICSGVTTSLHVIICWVLVLKSG 203

Query: 212 LQIRGAALANSISYWINAFLTALYVKTSSCCSETWTGFTVDALHNVLAFLRISIPSALMI 271
           L  RGAA+AN+ISYW+N  L + YVK S  CS TWTGF+ +A  +++ F+++ IPSA M+
Sbjct: 204 LGFRGAAVANAISYWLNVILLSCYVKFSPSCSLTWTGFSKEARRDIIPFMKLVIPSAFMV 263

Query: 272 C----------------------------------------AAVRVSNELGAGHPQAART 331
           C                                        A+ RVSNELG+G+P+ A+ 
Sbjct: 264 CSLEMWSFELLVLSSGLLPNPVLETSCPRTVWMIPFGLSGAASTRVSNELGSGNPKGAKL 323

Query: 332 AAYVALVMVVTEGVLVGFLMILLREIWGRVYSDEEEVVRYLATMMPTVAVSSFLDGVQSV 355
           A  V L   + E +LVG ++IL+R+IWG  YS + EVV ++A+M+P +A+   LD  Q+V
Sbjct: 324 AVRVVLSFSIVESILVGTVLILIRKIWGFAYSSDPEVVSHVASMLPILALGHSLDSFQTV 383

BLAST of Spg021932 vs. TAIR 10
Match: AT1G73700.1 (MATE efflux family protein )

HSP 1 Score: 323.2 bits (827), Expect = 2.7e-88
Identity = 177/369 (47.97%), Postives = 241/369 (65.31%), Query Frame = 0

Query: 32  VLNEVKRQLLLAGPLFSVGLLQYCLQIISLMFVGHLGELALAAASMATSFVSVTGFSLLM 91
           V  EVK+QL L+ PL  V LLQY LQ+IS+MFVGHLG L L+AAS+ATSF SVTGF+ L+
Sbjct: 21  VKEEVKKQLWLSAPLIGVSLLQYSLQVISVMFVGHLGSLPLSAASIATSFASVTGFTFLL 80

Query: 92  GMASALDTLCGQSFGAKRYHMLGIHTQCAMFVLLIVSILIAIVSSNTKMIPIALHQDQEI 151
           G ASAL+TLCGQ++GAK Y  LGI  Q AMFVLLI+S+ ++I+ +NT+ I + +HQD+ I
Sbjct: 81  GTASALETLCGQAYGAKLYGKLGIQMQRAMFVLLILSVPLSIIWANTEQILVLVHQDKSI 140

Query: 152 SEGAGLYARYMIPSVVAYGQLQCLVKFLQTQNIVFPMVLSSGVAALIHILLCWILVSKFG 211
           +  AG YA+YMIPS+ AYG LQC+ +FLQ QN VFP+ + SG+   +H+LLCW+ V K G
Sbjct: 141 ASVAGSYAKYMIPSLFAYGLLQCINRFLQAQNNVFPVFVCSGITTCLHLLLCWLFVLKTG 200

Query: 212 LQIRGAALANSISYWINAFLTALYVKTSSCCSETWTGFTVDALHNVLAFLRISIPSALMI 271
           L  RGAALA S+SYW N  L + YVK S  CS +WTGF+ +A   +  F +I+ PSA+M+
Sbjct: 201 LGYRGAALAISVSYWFNVILLSCYVKFSPSCSHSWTGFSKEAFQELYDFSKIAFPSAVMV 260

Query: 272 C----------------------------------------------AAVRVSNELGAGH 331
           C                                              A++RVSNELGAG+
Sbjct: 261 CLELWSFELLVLASGLLPNPVLETSVLSICLNTSLTIWQISVGLGGAASIRVSNELGAGN 320

Query: 332 PQAARTAAYVALVMVVTEGVLVGFLMILLREIWGRVYSDEEEVVRYLATMMPTVAVSSFL 355
           PQ A+ A YV + + V EG++V  +++ +R+I G  +S + +++ Y A+M+P VA  +FL
Sbjct: 321 PQVAKLAVYVIVGIAVAEGIVVVTVLLSIRKILGHAFSSDPKIIAYAASMIPIVACGNFL 380

BLAST of Spg021932 vs. TAIR 10
Match: AT1G71140.1 (MATE efflux family protein )

HSP 1 Score: 238.8 bits (608), Expect = 6.7e-63
Identity = 140/368 (38.04%), Postives = 212/368 (57.61%), Query Frame = 0

Query: 33  LNEVKRQLLLAGPLFSVGLLQYCLQIISLMFVGHLGELALAAASMATSFVSVTGFSLLMG 92
           L E K+   +AGP+ +V    Y LQ+IS+M VGHLGEL L++ ++A SF SVTGFS++ G
Sbjct: 24  LRETKKLSYIAGPMIAVNSSMYVLQVISIMMVGHLGELFLSSTAIAVSFCSVTGFSVVFG 83

Query: 93  MASALDTLCGQSFGAKRYHMLGIHTQCAMFVLLIVSILIAIVSSNTKMIPIALHQDQEIS 152
           +ASAL+TLCGQ+ GAK+Y  LG+HT   +  L +V I ++++ +    I   + QD  ++
Sbjct: 84  LASALETLCGQANGAKQYEKLGVHTYTGIVSLFLVCIPLSLLWTYIGDILSLIGQDAMVA 143

Query: 153 EGAGLYARYMIPSVVAYGQLQCLVKFLQTQNIVFPMVLSSGVAALIHILLCWILVSKFGL 212
           + AG +A ++IP++  Y  LQ LV+F Q Q+++ P+V+SS  +  IHI+LCW LV KFGL
Sbjct: 144 QEAGKFATWLIPALFGYATLQPLVRFFQAQSLILPLVMSSVSSLCIHIVLCWSLVFKFGL 203

Query: 213 QIRGAALANSISYWINAFLTALYVKTSSCCSETWTGFTVDALHNVLAFLRISIPSALMIC 272
              GAA+A  +SYW+N  +  LY+  SS CS++    ++     +  F R  IPSA MIC
Sbjct: 204 GSLGAAIAIGVSYWLNVTVLGLYMTFSSSCSKSRATISMSLFEGMGEFFRFGIPSASMIC 263

Query: 273 ----------------------------------------------AAVRVSNELGAGHP 332
                                                         A+ RV+NELGAG+P
Sbjct: 264 LEWWSFEFLVLLSGILPNPKLEASVLSVCLSTQSSLYQIPESLGAAASTRVANELGAGNP 323

Query: 333 QAARTAAYVALVMVVTEGVLVGFLMILLREIWGRVYSDEEEVVRYLATMMPTVAVSSFLD 355
           + AR A Y A+V+   E ++VG ++   R ++G ++S E EVV Y+ +M P +++S   D
Sbjct: 324 KQARMAVYTAMVITGVESIMVGAIVFGARNVFGYLFSSETEVVDYVKSMAPLLSLSVIFD 383

BLAST of Spg021932 vs. TAIR 10
Match: AT1G15150.1 (MATE efflux family protein )

HSP 1 Score: 236.9 bits (603), Expect = 2.5e-62
Identity = 137/381 (35.96%), Postives = 216/381 (56.69%), Query Frame = 0

Query: 20  EKRDMMYIQRGEVLNEVKRQLLLAGPLFSVGLLQYCLQIISLMFVGHLGELALAAASMAT 79
           EK     +Q G    E+KR +  A P+ +V ++Q+ +QIIS++ VGHLG L+LA+AS A 
Sbjct: 16  EKVTWRDLQDGSFTAELKRLICFAAPMAAVVIIQFMIQIISMVMVGHLGRLSLASASFAV 75

Query: 80  SFVSVTGFSLLMGMASALDTLCGQSFGAKRYHMLGIHTQCAMFVLLIVSILIAIVSSNTK 139
           SF +VTGFS ++G++ ALDTL GQ++GAK Y  LG+    AMF L +V + ++++  N  
Sbjct: 76  SFCNVTGFSFIIGLSCALDTLSGQAYGAKLYRKLGVQAYTAMFCLTLVCLPLSLLWFNMG 135

Query: 140 MIPIALHQDQEISEGAGLYARYMIPSVVAYGQLQCLVKFLQTQNIVFPMVLSSGVAALIH 199
            + + L QD  I+  AG YA ++IP + AY  LQ L+++ + Q+++ P++++S V   IH
Sbjct: 136 KLIVILGQDPAIAHEAGRYAAWLIPGLFAYAVLQPLIRYFKNQSLITPLLVTSSVVFCIH 195

Query: 200 ILLCWILVSKFGLQIRGAALANSISYWINAFLTALYVKTSSCCSETWTGFTVDALHNVLA 259
           + LCW+LV K GL   G ALA S+SYW+ A     ++  SS CSET    T++    V  
Sbjct: 196 VPLCWLLVYKSGLGHIGGALALSLSYWLYAIFLGSFMYYSSACSETRAPLTMEIFEGVRE 255

Query: 260 FLRISIPSALMIC----------------------------------------------A 319
           F++ ++PSA M+C                                              A
Sbjct: 256 FIKYALPSAAMLCLEWWSYELIILLSGLLPNPQLETSVLSICFETLSITYSIPLAIAAAA 315

Query: 320 AVRVSNELGAGHPQAARTAAYVALVMVVTEGVLVGFLMILLREIWGRVYSDEEEVVRYLA 355
           + R+SNELGAG+ +AA    Y A+ + V + ++V   ++  R ++G V+S +++ + Y+A
Sbjct: 316 STRISNELGAGNSRAAHIVVYAAMSLAVMDALMVSMSLLAGRHVFGHVFSSDKKTIEYVA 375