Spg021905 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg021905
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Description(1->3)-beta-glucan endohydrolase
Locationscaffold2: 13006529 .. 13009811 (+)
RNA-Seq ExpressionSpg021905
SyntenySpg021905
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCATCAATCTTCTCCAGGTTTTGCTCCGTTTCTGTGCTTCTCCTTGGGGTTTTGATTTCTACGGGTTTCCACCAAGTATTAGGTAATTTTCCTTTAATCTCTCTGTTTTCGATTGAAGAACCGATTGTCGATTGGCTCGATTTTTCTAGGGTTTTTAATCGCATCGTAAGGATTCTGAATCGTTATGGTGAAGCAGGATCGAAGTTGGGAAATATGGCGATGGCGATTGAAGATGAAGATGAAGATTCCATGGCTCTTTTACCCTTTGGATCTTCCTCTGGCATTGGCAGCTTCGTTCGTCTTCCTCAATGCGGTGGCCCTCGCCGAACAGGTACTCTTTCCCCTCACTTGTTTCTGCTTCGTTTCCCCTTCTTATGGATGCATTTCTGCAAAAAAAAAAAGAAAAATTTTGCGTTTATATCATCAAGTTTGGAGAAATTATTCGGTGAGACCGCTGCAACGTGGCGGATAGTTTTCATGTAAGAGAGAAATTGTAAACATTAACTTTGTATATCCAACGATCTTTAGGAGCAGACAAATGTTAATATATACATCCGACGGTTTTAAAAGAGAATGCCACATGATTTAATAATTTGTCCCAATTTTGAGCTTCTTGTTTCTCAATGGCATTCTCAGGTTTTAGCTAAAATTTCTATCGCATTCTTAAATCTTCATGGCAAATTTCCATATTTGAGTTTATACAATTTGGTTTTTTTTTTCTTGACGACATGTGAGTCGGGAGGTTTGAACTTTTGACCTCTTGGTCGAGAATACAAGCTCTATATACCGGTCGAGTTGTTGGCCATCAATATGGGTTTTGGTTTTGTTTTTTTGGATTTAAAAATAAGTTTACAAAACACTAATTCCAACTATGTATTACTTTTATCTGCTATCTACATTTTAAAAACATTTTTAAAATTTAAGTCTAAATTTGAAAACTGAAAAGTGGTTTTTAATGCTCCCTTTGATAATCATCTCACTTTTTATTTTTGGTTTTTGAAAATTTATGTTTTTTTTTTTCCTCTCCATTTCCTTACCTTGGTTTTCATTTTTAGTAAGAAAACATTTGAATTATTAGCCAAGTTCAAAAAACAAAAACAAGTTCTTGAAAACTGCGGCCCCATTTGATAACCATTTGGTTTTTCAAAATTATGCTTATTTTCTCTCAATTTCCTCACCATGATTTTCATTTTTGTTAAAGAGACATTATAGTTCTTTGTCAAATTCTAAAAACAAAAACAAGTTTTTGAAAACTACTTTTTTTAAAAAAAAAAAATTGACTTTGTTTTTGAAAACATGAGAAGGATGATGTAGATAACAACCCAAAGAAATGTATGGGTGAAAAGAGTGTTTATAAGCTAAATTTTCAAAAACCAAAAACCAAAAACGAAATGGTTATCAAACGGGGCCTACTTTTTTTAGTTTTCAAAACTTGACTTGGTTTTTAAAAATTTGGAAAGATGGTAGATATCAAAACAAAGAAACTTATAATGGAAGTAGTGATTGTAAACTTAAATTTCAGAAACCATTTCAAAAACAAAAAACCAAAGGCTTATCAAACAAGGCCTAAAATGTTTGTTTCTGTCCTTATAAAAAGAAAATTAAAGTGTTTATGATATTTGATTAACAATTCAAATATGTTCTTAAGAAAAGTGAAAAACATATCAATGGTAAGGAAGTAAAAGTAATTTAAAAACAGAAAACTAGTTTGCAACTGTGCTATATTTGCTATTAATTTGGTCCAATTTGTCATCTATGCAACTGCTCCATTTTTATTTTTTTTATTGGACCGAATACTCATTTGGGTTTCATGAGCTCTAATACCATATTAAATAACATGGGGTAACTCATCTATCTTATGAATTGGGAGGTCTCCACTAATCTTTTCAATGTGGGATCCTCAACATCTTCAAACATCTCAATCCTTGTACTACCCTTAAATCTTTTCTAGATCAAAAGAGGAAGAAGACCCTACAATTTCAAATACTATCAAAAGGATTTGGTGAGCGCATGCATGTGTCATATTTACATCCACACCTACAAGCATATCCCACGAGTTTGGTTGTTTCAAACGTTTCTCATTCTCAAAATCTGTAATTTACTTTTCTTCCATTATTGTTGACATCATGGTTTGATTTGCATTTTACCTTCTTGTCAAACATTTTGGTGATAAAACGAGTCAGAATTGTTATCTATCAATACCAACAAGATGCATTTATTTATTTGATTTGGGCTGGCTAAACAATTTATGCTGCATGAATCTCTGTTCTCTTCCTTCCCAACAGATAAACGTTACTTTTGTTTGAGCACTCGTATGGTTACAATTACAGAATCCATTGGTGTTTGCTATTCAAGCTCAGGCAACAATCTACCAGATGCCAGAGAAGTAGTACAACTATACAAAAGCAACGGCATCGAAAAGATGAGGATCTATAATCCTGACGCAGCTATGTTAAACGCTCTTCGAGGATCGAACATCGAAGTCATCGTAGGCATCCCGAATACTGACCTCGAACACATCGTTAATCTTTCTTCTGCAATGAATTGGGTCCAAAGGAATATTCAAGCCTACATGGCAGACGTCAAATTCCGATACATTGCCATCGGGAACGAAGTGCAGCCATCCGATGCCGTTGCTCGATACGTTTTACCAGCCATGAACAGCATTTATAGCGCAATTTTAGCAGCCAATTTGCAGGATCAAATAAAGGTTTCCACAGTAATATCCATCAGCTTCTTGTCGAACTCCTCCTTTCCTCCATCTTTTAGCTCATTCAGCTCAGAGGCTGGTGGATTTATGGAACCAATCGTTAACTTTCTGGCTAAGAATGGTTCACCACTTCTTGCCAATGTGTATCCCTACTTCACCTATATCGATATCACTCGAACCATCAGCCTTGACTATGCTTTGTTCATGCAATCACCGGCTCTTACGATAAGAGATGGCAATTTTGAGTACAACAGTCTTTTCGAAGCAATGGTCGATGCTCTTTACGTAGCTCTCGAGAAGTCGGGTGGAGCGGAAGTGTCGATCGTGATATCGGAGAGCGGTTGGCCATCGGGTGGAGGCACGGCTGCTACAGTTGAGAATGCAGGTACATACTATAGAAATTTGATTAGCTTTGTTCGAGGTGGGACTCCGAGAAGGCCTAGAAGAGCTATTGAAACGTATTTGTTTGCTATGTTTGATGAAAATAACAAGGCTCCTGAAATGGAGAAACATTTTGGTTTATTCACAGCTGACAAGAAATCAAAGTACCAACTTAGATTTAGTTAA

mRNA sequence

ATGGCTTCATCAATCTTCTCCAGGTTTTGCTCCGTTTCTGTGCTTCTCCTTGGGGTTTTGATTTCTACGGGTTTCCACCAAGTATTAGGTAATTTTCCTTTAATCTCTCTGTTTTCGATTGAAGAACCGATTGTCGATTGGCTCGATTTTTCTAGGGTTTTTAATCGCATCGTAAGGATTCTGAATCGTTATGGTGAAGCAGGATCGAAGTTGGGAAATATGGCGATGGCGATTGAAGATGAAGATGAAGATTCCATGGCTCTTTTACCCTTTGGATCTTCCTCTGGCATTGGCAGCTTCGTTCGTCTTCCTCAATGCGGTGGCCCTCGCCGAACAGGTACTCTTTCCCCTCACTTGTTTCTGCTTCGTTTCCCCTTCTTATGGATGCATTTCTGCAAAAAAAAAAAGAAAAATTTTGCGTTTATATCATCAAGTTTGGAGAAATTATTCGATAAACGTTACTTTTGTTTGAGCACTCGTATGGTTACAATTACAGAATCCATTGGTGTTTGCTATTCAAGCTCAGGCAACAATCTACCAGATGCCAGAGAAGTAGTACAACTATACAAAAGCAACGGCATCGAAAAGATGAGGATCTATAATCCTGACGCAGCTATGTTAAACGCTCTTCGAGGATCGAACATCGAAGTCATCGTAGGCATCCCGAATACTGACCTCGAACACATCGTTAATCTTTCTTCTGCAATGAATTGGGTCCAAAGGAATATTCAAGCCTACATGGCAGACGTCAAATTCCGATACATTGCCATCGGGAACGAAGTGCAGCCATCCGATGCCGTTGCTCGATACGTTTTACCAGCCATGAACAGCATTTATAGCGCAATTTTAGCAGCCAATTTGCAGGATCAAATAAAGGTTTCCACAGTAATATCCATCAGCTTCTTGTCGAACTCCTCCTTTCCTCCATCTTTTAGCTCATTCAGCTCAGAGGCTGGTGGATTTATGGAACCAATCGTTAACTTTCTGGCTAAGAATGGTTCACCACTTCTTGCCAATGTGTATCCCTACTTCACCTATATCGATATCACTCGAACCATCAGCCTTGACTATGCTTTGTTCATGCAATCACCGGCTCTTACGATAAGAGATGGCAATTTTGAGTACAACAGTCTTTTCGAAGCAATGGTCGATGCTCTTTACGTAGCTCTCGAGAAGTCGGGTGGAGCGGAAGTGTCGATCGTGATATCGGAGAGCGGTTGGCCATCGGGTGGAGGCACGGCTGCTACAGTTGAGAATGCAGGTACATACTATAGAAATTTGATTAGCTTTGTTCGAGGTGGGACTCCGAGAAGGCCTAGAAGAGCTATTGAAACGTATTTGTTTGCTATGTTTGATGAAAATAACAAGGCTCCTGAAATGGAGAAACATTTTGGTTTATTCACAGCTGACAAGAAATCAAAGTACCAACTTAGATTTAGTTAA

Coding sequence (CDS)

ATGGCTTCATCAATCTTCTCCAGGTTTTGCTCCGTTTCTGTGCTTCTCCTTGGGGTTTTGATTTCTACGGGTTTCCACCAAGTATTAGGTAATTTTCCTTTAATCTCTCTGTTTTCGATTGAAGAACCGATTGTCGATTGGCTCGATTTTTCTAGGGTTTTTAATCGCATCGTAAGGATTCTGAATCGTTATGGTGAAGCAGGATCGAAGTTGGGAAATATGGCGATGGCGATTGAAGATGAAGATGAAGATTCCATGGCTCTTTTACCCTTTGGATCTTCCTCTGGCATTGGCAGCTTCGTTCGTCTTCCTCAATGCGGTGGCCCTCGCCGAACAGGTACTCTTTCCCCTCACTTGTTTCTGCTTCGTTTCCCCTTCTTATGGATGCATTTCTGCAAAAAAAAAAAGAAAAATTTTGCGTTTATATCATCAAGTTTGGAGAAATTATTCGATAAACGTTACTTTTGTTTGAGCACTCGTATGGTTACAATTACAGAATCCATTGGTGTTTGCTATTCAAGCTCAGGCAACAATCTACCAGATGCCAGAGAAGTAGTACAACTATACAAAAGCAACGGCATCGAAAAGATGAGGATCTATAATCCTGACGCAGCTATGTTAAACGCTCTTCGAGGATCGAACATCGAAGTCATCGTAGGCATCCCGAATACTGACCTCGAACACATCGTTAATCTTTCTTCTGCAATGAATTGGGTCCAAAGGAATATTCAAGCCTACATGGCAGACGTCAAATTCCGATACATTGCCATCGGGAACGAAGTGCAGCCATCCGATGCCGTTGCTCGATACGTTTTACCAGCCATGAACAGCATTTATAGCGCAATTTTAGCAGCCAATTTGCAGGATCAAATAAAGGTTTCCACAGTAATATCCATCAGCTTCTTGTCGAACTCCTCCTTTCCTCCATCTTTTAGCTCATTCAGCTCAGAGGCTGGTGGATTTATGGAACCAATCGTTAACTTTCTGGCTAAGAATGGTTCACCACTTCTTGCCAATGTGTATCCCTACTTCACCTATATCGATATCACTCGAACCATCAGCCTTGACTATGCTTTGTTCATGCAATCACCGGCTCTTACGATAAGAGATGGCAATTTTGAGTACAACAGTCTTTTCGAAGCAATGGTCGATGCTCTTTACGTAGCTCTCGAGAAGTCGGGTGGAGCGGAAGTGTCGATCGTGATATCGGAGAGCGGTTGGCCATCGGGTGGAGGCACGGCTGCTACAGTTGAGAATGCAGGTACATACTATAGAAATTTGATTAGCTTTGTTCGAGGTGGGACTCCGAGAAGGCCTAGAAGAGCTATTGAAACGTATTTGTTTGCTATGTTTGATGAAAATAACAAGGCTCCTGAAATGGAGAAACATTTTGGTTTATTCACAGCTGACAAGAAATCAAAGTACCAACTTAGATTTAGTTAA

Protein sequence

MASSIFSRFCSVSVLLLGVLISTGFHQVLGNFPLISLFSIEEPIVDWLDFSRVFNRIVRILNRYGEAGSKLGNMAMAIEDEDEDSMALLPFGSSSGIGSFVRLPQCGGPRRTGTLSPHLFLLRFPFLWMHFCKKKKKNFAFISSSLEKLFDKRYFCLSTRMVTITESIGVCYSSSGNNLPDAREVVQLYKSNGIEKMRIYNPDAAMLNALRGSNIEVIVGIPNTDLEHIVNLSSAMNWVQRNIQAYMADVKFRYIAIGNEVQPSDAVARYVLPAMNSIYSAILAANLQDQIKVSTVISISFLSNSSFPPSFSSFSSEAGGFMEPIVNFLAKNGSPLLANVYPYFTYIDITRTISLDYALFMQSPALTIRDGNFEYNSLFEAMVDALYVALEKSGGAEVSIVISESGWPSGGGTAATVENAGTYYRNLISFVRGGTPRRPRRAIETYLFAMFDENNKAPEMEKHFGLFTADKKSKYQLRFS
Homology
BLAST of Spg021905 vs. NCBI nr
Match: XP_004139045.1 (glucan endo-1,3-beta-glucosidase [Cucumis sativus] >KAE8653516.1 hypothetical protein Csa_006933 [Cucumis sativus])

HSP 1 Score: 582.4 bits (1500), Expect = 3.6e-162
Identity = 326/483 (67.49%), Postives = 352/483 (72.88%), Query Frame = 0

Query: 1   MASS--IFSRFCSVSVLLLGVLISTGFHQVLGNFPLISLFSIEEPIVDWLDFSRVFNRIV 60
           MASS   FS F  V VLLLGVLIS G                             FNR+V
Sbjct: 1   MASSSIFFSSFSFVLVLLLGVLISPG-----------------------------FNRLV 60

Query: 61  RILNRYGEAGSKLGNMAMAIEDEDEDSMALLPF-GSSSGIGSFVRLPQCGGPRRTGTLSP 120
                    G KLGNMAMA+  EDEDSMALLPF GS+S +G F+RLPQCGGPRR      
Sbjct: 61  ---------GLKLGNMAMAMTIEDEDSMALLPFGGSASSVGRFIRLPQCGGPRR------ 120

Query: 121 HLFLLRFPFLWMHFCKKKKKNFAFISSSLEKLFDKRYFCLSTRMVTITESIGVCYSSSGN 180
                                                          TESIGVCYSS+GN
Sbjct: 121 -----------------------------------------------TESIGVCYSSAGN 180

Query: 181 NLPDAREVVQLYKSNGIEKMRIYNPDAAMLNALRGSNIEVIVGIPNTDLEHIVNLSSAMN 240
           NLPDAREVVQLYK+N I+KMRIYNPD  +LNALRGSNIEVIVGIPNT +EHIVNLSSA N
Sbjct: 181 NLPDAREVVQLYKNNSIKKMRIYNPDTTILNALRGSNIEVIVGIPNTYIEHIVNLSSAAN 240

Query: 241 WVQRNIQAYMADVKFRYIAIGNEVQPSDAVARYVLPAMNSIYSAILAANLQDQIKVSTVI 300
           WVQ+NIQAY+  V FRYIA+GNEV+P DA+A+YVLPAM+SIYSAI AANLQDQIKVSTVI
Sbjct: 241 WVQKNIQAYVPYVNFRYIAVGNEVKPPDAIAQYVLPAMSSIYSAISAANLQDQIKVSTVI 300

Query: 301 SISFLSNSSFPPSFSSFSSEAGGFMEPIVNFLAKNGSPLLANVYPYFTYIDITRTISLDY 360
           S+SFLSNSSFPPS  SFSSEAGGFMEPI+ FLAKNGSPLLANVYPYF Y +I +TISLDY
Sbjct: 301 SMSFLSNSSFPPSNGSFSSEAGGFMEPIIKFLAKNGSPLLANVYPYFVYTEINQTISLDY 360

Query: 361 ALFMQSPALTIRDGNFEYNSLFEAMVDALYVALEKSGGAEVSIVISESGWPSGGGTAATV 420
           ALFMQSPAL I DGNF+YNSLFE+MVDALYVALEK GGA+VSIVISESGWPSGGG  AT+
Sbjct: 361 ALFMQSPALVISDGNFKYNSLFESMVDALYVALEKLGGAKVSIVISESGWPSGGGNGATI 392

Query: 421 ENAGTYYRNLISFVRGGTPRRPRRAIETYLFAMFDENNKAPEMEKHFGLFTADKKSKYQL 480
           ENAGTYYRNLISFVR GTPRRPRRAIETYLFAMFDEN K+ EMEKHFGLFT DKKSKYQL
Sbjct: 421 ENAGTYYRNLISFVRNGTPRRPRRAIETYLFAMFDENLKSLEMEKHFGLFTPDKKSKYQL 392

BLAST of Spg021905 vs. NCBI nr
Match: XP_022961205.1 (glucan endo-1,3-beta-glucosidase-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 575.1 bits (1481), Expect = 5.7e-160
Identity = 311/430 (72.33%), Postives = 334/430 (77.67%), Query Frame = 0

Query: 51  SRVFNRIVRILNRYGEAGSKLGNMAMAIEDEDEDSMALLPFGSSSGIGSFVRLPQCGGPR 110
           S VF+R +         G K GNM MAI  ED DSMALLPFGS+S IGSF RLPQCGGPR
Sbjct: 15  SLVFHRFI---------GLKSGNMVMAI--EDVDSMALLPFGSASRIGSFFRLPQCGGPR 74

Query: 111 RTGTLSPHLFLLRFPFLWMHFCKKKKKNFAFISSSLEKLFDKRYFCLSTRMVTITESIGV 170
           R                                                     TESIGV
Sbjct: 75  R-----------------------------------------------------TESIGV 134

Query: 171 CYSSSGNNLPDAREVVQLYKSNGIEKMRIYNPDAAMLNALRGSNIEVIVGIPNTDLEHIV 230
           CY++ GNNLPDAREVVQLYK +GIEKMRIYNPD A+LNALRGSNIEVIVGIPNTDLE IV
Sbjct: 135 CYTNLGNNLPDAREVVQLYKIHGIEKMRIYNPDTAILNALRGSNIEVIVGIPNTDLERIV 194

Query: 231 NLSSAMNWVQRNIQAYMADVKFRYIAIGNEVQPSDAVARYVLPAMNSIYSAILAANLQDQ 290
           NLSSA NWVQRNIQAY+  +KFRY+A+GN+VQPSD++ARYVLPA++SI SAI AANLQDQ
Sbjct: 195 NLSSASNWVQRNIQAYVPHIKFRYVAVGNDVQPSDSIARYVLPAISSINSAISAANLQDQ 254

Query: 291 IKVSTVISISFLSNSSFPPSFSSFSSEAGGFMEPIVNFLAKNGSPLLANVYPYFTYIDIT 350
           IKVSTVISIS LSNSSFPPS+SSFSSEA GF+EPIV+FLAKNGSPLLANVYPYFTY DIT
Sbjct: 255 IKVSTVISISLLSNSSFPPSYSSFSSEASGFIEPIVHFLAKNGSPLLANVYPYFTYTDIT 314

Query: 351 RTISLDYALFMQSPALTIRDGNFEYNSLFEAMVDALYVALEKSGGAEVSIVISESGWPSG 410
           RTISLDYALFMQSP L IRD NFEYNSLFE+MVDALYVALEKSGGAEVSIVI+ESGWPS 
Sbjct: 315 RTISLDYALFMQSPTLVIRDDNFEYNSLFESMVDALYVALEKSGGAEVSIVIAESGWPSN 374

Query: 411 GGTAATVENAGTYYRNLISFVRGGTPRRPRRAIETYLFAMFDENNKAPEMEKHFGLFTAD 470
           G  AATVENAGTYYRNL++FV  GTPRRPRRAIETYLFAMFDEN K  EMEKHFGLFTAD
Sbjct: 375 GSAAATVENAGTYYRNLMNFVPTGTPRRPRRAIETYLFAMFDENLKPVEMEKHFGLFTAD 380

Query: 471 KKSKYQLRFS 481
           KKSKYQL FS
Sbjct: 435 KKSKYQLSFS 380

BLAST of Spg021905 vs. NCBI nr
Match: XP_022154372.1 (glucan endo-1,3-beta-glucosidase-like [Momordica charantia])

HSP 1 Score: 575.1 bits (1481), Expect = 5.7e-160
Identity = 317/480 (66.04%), Postives = 347/480 (72.29%), Query Frame = 0

Query: 1   MASSIFSRFCSVSVLLLGVLISTGFHQVLGNFPLISLFSIEEPIVDWLDFSRVFNRIVRI 60
           MASSIFS+FCSVS+LLLG LIS GF                          R+F      
Sbjct: 1   MASSIFSKFCSVSLLLLGALISAGF--------------------------RIF------ 60

Query: 61  LNRYGEAGSKLGNMAMAIEDEDEDSMALLPFGSSSGIGSFVRLPQCGGPRRTGTLSPHLF 120
                  G K GNMAMA+  E+ DSMALLPFGS+SGI SFVRLPQCGGPR          
Sbjct: 61  -------GLKSGNMAMALAIEEVDSMALLPFGSASGIDSFVRLPQCGGPR---------- 120

Query: 121 LLRFPFLWMHFCKKKKKNFAFISSSLEKLFDKRYFCLSTRMVTITESIGVCYSSSGNNLP 180
                                                     T+ ES+GVCY+SSG+NLP
Sbjct: 121 -----------------------------------------PTVAESVGVCYASSGSNLP 180

Query: 181 DAREVVQLYKSNGIEKMRIYNPDAAMLNALRGSNIEVIVGIPNTDLEHIVNLSSAMNWVQ 240
            A EVVQLYK+N I KMRIYNP  A+LNAL+GSNIEV+VGIPNTDLEH+VNLSSA NWVQ
Sbjct: 181 SATEVVQLYKNNSIGKMRIYNPSEAILNALQGSNIEVVVGIPNTDLEHVVNLSSAANWVQ 240

Query: 241 RNIQAYMADVKFRYIAIGNEVQPSDAVARYVLPAMNSIYSAILAANLQDQIKVSTVISIS 300
           RNIQAY++ VKFRYIAIGN+V PSD+ A YVLPA+ SIY AI AANLQDQIKVSTVISIS
Sbjct: 241 RNIQAYISHVKFRYIAIGNDVHPSDSFAGYVLPAIRSIYGAISAANLQDQIKVSTVISIS 300

Query: 301 FLSNSSFPPSFSSFSSEAGGFMEPIVNFLAKNGSPLLANVYPYFTYIDITRTISLDYALF 360
            LSNSSFPPS  SFSSEA GF+EPIV+FLA+NGSPLLANVYPYFTY DI RTISLDYALF
Sbjct: 301 LLSNSSFPPSIGSFSSEARGFIEPIVSFLAENGSPLLANVYPYFTYTDIARTISLDYALF 360

Query: 361 MQSPALTIRDGNFEYNSLFEAMVDALYVALEKSGGAEVSIVISESGWPSGGGTAATVENA 420
           MQSPAL IRDGNF YNSLFE+MVDALY ALEKSGG EVSIVISESGWPS  GTAAT+ENA
Sbjct: 361 MQSPALAIRDGNFMYNSLFESMVDALYAALEKSGGNEVSIVISESGWPSSEGTAATIENA 390

Query: 421 GTYYRNLISFVRGGTPRRPRRAIETYLFAMFDENNKAPEMEKHFGLFTADKKSKYQLRFS 480
           GTYYRNLI+FV+ GTPRRP RAIETYLF+MFDEN K PE+EKHFGLFT +KK KYQL FS
Sbjct: 421 GTYYRNLINFVQSGTPRRPGRAIETYLFSMFDENLKPPEIEKHFGLFTPNKKPKYQLSFS 390

BLAST of Spg021905 vs. NCBI nr
Match: XP_038880622.1 (glucan endo-1,3-beta-glucosidase-like [Benincasa hispida])

HSP 1 Score: 574.3 bits (1479), Expect = 9.7e-160
Identity = 308/431 (71.46%), Postives = 335/431 (77.73%), Query Frame = 0

Query: 50  FSRVFNRIVRILNRYGEAGSKLGNMAMAIEDEDEDSMALLPFGSSSGIGSFVRLPQCGGP 109
           FS   +  V +L  +    SK GNM MAI  EDEDSMALLPFGS+SG GSF+RL QCGGP
Sbjct: 8   FSTFCSVFVLLLGAFIPPESKSGNMVMAI--EDEDSMALLPFGSASGAGSFIRLSQCGGP 67

Query: 110 RRTGTLSPHLFLLRFPFLWMHFCKKKKKNFAFISSSLEKLFDKRYFCLSTRMVTITESIG 169
           RR                                                     TESIG
Sbjct: 68  RR-----------------------------------------------------TESIG 127

Query: 170 VCYSSSGNNLPDAREVVQLYKSNGIEKMRIYNPDAAMLNALRGSNIEVIVGIPNTDLEHI 229
           VCYSS+GNNLPDA+EVVQLYKSN I+KMRIYNPDAA+LNALRGSNIE+ VGIPNTD+EHI
Sbjct: 128 VCYSSAGNNLPDAKEVVQLYKSNDIKKMRIYNPDAAILNALRGSNIELTVGIPNTDIEHI 187

Query: 230 VNLSSAMNWVQRNIQAYMADVKFRYIAIGNEVQPSDAVARYVLPAMNSIYSAILAANLQD 289
           VNLSSA NWV++NI AY+  VKFRY+AIGNEVQPSD ++RYVLPAM+SI+SAI AANLQD
Sbjct: 188 VNLSSAANWVEKNILAYVPLVKFRYVAIGNEVQPSDPISRYVLPAMSSIHSAISAANLQD 247

Query: 290 QIKVSTVISISFLSNSSFPPSFSSFSSEAGGFMEPIVNFLAKNGSPLLANVYPYFTYIDI 349
           QIK+STVIS SFLSNSSFPPS  SFSSEAGGFMEPIV+FL KNGSPLLANVYPYF Y +I
Sbjct: 248 QIKISTVISTSFLSNSSFPPSNGSFSSEAGGFMEPIVDFLVKNGSPLLANVYPYFVYTEI 307

Query: 350 TRTISLDYALFMQSPALTIRDGNFEYNSLFEAMVDALYVALEKSGGAEVSIVISESGWPS 409
           TRTISLDYALFMQSP+L IRDGNF YNSLFE+MVDALYVALEK GG +VSIVISESGWPS
Sbjct: 308 TRTISLDYALFMQSPSLVIRDGNFFYNSLFESMVDALYVALEKLGGDKVSIVISESGWPS 367

Query: 410 GGGTAATVENAGTYYRNLISFVRGGTPRRPRRAIETYLFAMFDENNKAPEMEKHFGLFTA 469
           GGG  ATVENAGTYYRNLISFVR GTPRRPRRAIETYLFAMFDEN K+ EMEKHFGLFT 
Sbjct: 368 GGGKGATVENAGTYYRNLISFVRTGTPRRPRRAIETYLFAMFDENLKSLEMEKHFGLFTP 383

Query: 470 DKKSKYQLRFS 481
           DKKSK+QL FS
Sbjct: 428 DKKSKFQLSFS 383

BLAST of Spg021905 vs. NCBI nr
Match: KAG6590190.1 (Lichenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 573.5 bits (1477), Expect = 1.7e-159
Identity = 306/413 (74.09%), Postives = 328/413 (79.42%), Query Frame = 0

Query: 68  GSKLGNMAMAIEDEDEDSMALLPFGSSSGIGSFVRLPQCGGPRRTGTLSPHLFLLRFPFL 127
           G K GNM MAI  ED DSMALLPFGS+S IGSF RLPQCGGPRR                
Sbjct: 7   GLKSGNMVMAI--EDVDSMALLPFGSASRIGSFFRLPQCGGPRR---------------- 66

Query: 128 WMHFCKKKKKNFAFISSSLEKLFDKRYFCLSTRMVTITESIGVCYSSSGNNLPDAREVVQ 187
                                                TESIGVCY++ GNNLPDAREVVQ
Sbjct: 67  -------------------------------------TESIGVCYTNLGNNLPDAREVVQ 126

Query: 188 LYKSNGIEKMRIYNPDAAMLNALRGSNIEVIVGIPNTDLEHIVNLSSAMNWVQRNIQAYM 247
           LYK +GIEKMRIYNPD A+LNALRG NIEVIVGIPNTDLE IVNLSSA NWVQRNIQAY+
Sbjct: 127 LYKIHGIEKMRIYNPDTAILNALRGLNIEVIVGIPNTDLERIVNLSSASNWVQRNIQAYV 186

Query: 248 ADVKFRYIAIGNEVQPSDAVARYVLPAMNSIYSAILAANLQDQIKVSTVISISFLSNSSF 307
             +KFRY+A+GN+VQPSD++ARYVLPA++SI SAI AANLQDQIKVSTVISIS LSNSSF
Sbjct: 187 PHIKFRYVAVGNDVQPSDSIARYVLPAISSINSAISAANLQDQIKVSTVISISLLSNSSF 246

Query: 308 PPSFSSFSSEAGGFMEPIVNFLAKNGSPLLANVYPYFTYIDITRTISLDYALFMQSPALT 367
           PPS+SSFSSEA GF+EPIV+FLAKNGSPLLANVYPYFTY DI+RTISLDYALFMQSP L 
Sbjct: 247 PPSYSSFSSEASGFIEPIVHFLAKNGSPLLANVYPYFTYTDISRTISLDYALFMQSPTLV 306

Query: 368 IRDGNFEYNSLFEAMVDALYVALEKSGGAEVSIVISESGWPSGGGTAATVENAGTYYRNL 427
           IRDGNFEYNSLFE+MVDALYVALEKSGGAEVSIVI+ESGWPS G  AATVENAGTYYRNL
Sbjct: 307 IRDGNFEYNSLFESMVDALYVALEKSGGAEVSIVIAESGWPSNGSAAATVENAGTYYRNL 364

Query: 428 ISFVRGGTPRRPRRAIETYLFAMFDENNKAPEMEKHFGLFTADKKSKYQLRFS 481
           ++FV  GTPRRPRRAIETYLFAMFDEN K  EMEKHFGLFTADKKSKYQL FS
Sbjct: 367 MNFVPTGTPRRPRRAIETYLFAMFDENLKPVEMEKHFGLFTADKKSKYQLSFS 364

BLAST of Spg021905 vs. ExPASy Swiss-Prot
Match: A7PQW3 (Glucan endo-1,3-beta-glucosidase OS=Vitis vinifera OX=29760 GN=VIT_06s0061g00120 PE=1 SV=2)

HSP 1 Score: 367.9 bits (943), Expect = 1.8e-100
Identity = 193/328 (58.84%), Postives = 251/328 (76.52%), Query Frame = 0

Query: 161 MVTIT---ESIGVCYSSSGNNLPDAREVVQLYKSNGIEKMRIYNPDAAMLNALRGSNIEV 220
           M TIT   +SIGVCY  +GNNLP A +V+ LYKSNGI  MRIY+P++  L ALRGS+IE+
Sbjct: 20  MSTITIGAQSIGVCYGRNGNNLPSASQVINLYKSNGIGSMRIYDPNSDTLQALRGSDIEL 79

Query: 221 IVGIPNTDLEHIV-NLSSAMNWVQRNIQAYMADVKFRYIAIGNEVQPSDA---VARYVLP 280
           I+ +PNTDL+ +  + S+A  WVQ N+  Y ++VKFRYIA+GNEV P+ +    A+YVLP
Sbjct: 80  ILDVPNTDLQSLASDASAAATWVQNNVVNYASEVKFRYIAVGNEVLPTGSNAQYAQYVLP 139

Query: 281 AMNSIYSAILAANLQDQIKVSTVISISFLSNSSFPPSFSSFSSEAGGFMEPIVNFLAKNG 340
           AM ++ SAI +A LQDQIKVST  + S +   S+PPS  SFS +   F+ PI++FLA+NG
Sbjct: 140 AMKNVQSAITSAGLQDQIKVSTA-TFSAVLGKSYPPSEGSFSDDVSSFINPIISFLAENG 199

Query: 341 SPLLANVYPYFTYIDITRTISLDYALFMQSPALTIRDGNFEYNSLFEAMVDALYVALEKS 400
           SPLLAN+YPYF+Y   T+ I LDYALF  S  + ++DG+++Y +LF+A++DALY ALEK+
Sbjct: 200 SPLLANIYPYFSYTGDTQNIRLDYALFTAS-GVVVQDGSYQYQNLFDALLDALYAALEKA 259

Query: 401 GGAEVSIVISESGWPSGGGTAATVENAGTYYRNLISFVRGGTPRRPRRAIETYLFAMFDE 460
           GG+ + IV+SESGWPS GGTAATV+NA TYY+NLI+ V+GGTPR+   AIETYLFAMFDE
Sbjct: 260 GGSNLKIVVSESGWPSEGGTAATVDNARTYYKNLINHVKGGTPRK-SGAIETYLFAMFDE 319

Query: 461 NNKAP-EMEKHFGLFTADKKSKYQLRFS 481
           N K   E EKHFGLFT  ++SKYQ+ FS
Sbjct: 320 NQKTGLETEKHFGLFTPGQESKYQISFS 344

BLAST of Spg021905 vs. ExPASy Swiss-Prot
Match: P07979 (Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3)

HSP 1 Score: 356.3 bits (913), Expect = 5.4e-97
Identity = 178/327 (54.43%), Postives = 241/327 (73.70%), Query Frame = 0

Query: 157 LSTRMVTITESIGVCYSSSGNNLPDAREVVQLYKSNGIEKMRIYNPDAAMLNALRGSNIE 216
           +S+  +   +S+GVCY   GNNLP A +VVQLYKS  I +MR+Y+P+ A L ALRGSNIE
Sbjct: 22  VSSTEIVGAQSVGVCYGMLGNNLPPASQVVQLYKSKNIRRMRLYDPNQAALQALRGSNIE 81

Query: 217 VIVGIPNTDLEHI-VNLSSAMNWVQRNIQAYMADVKFRYIAIGNEVQP---SDAVARYVL 276
           V++G+PN+DL++I  N S+A NWVQRN++ +   VKFRYIA+GNEV P   + ++ RY+L
Sbjct: 82  VMLGVPNSDLQNIAANPSNANNWVQRNVRNFWPAVKFRYIAVGNEVSPVTGTSSLTRYLL 141

Query: 277 PAMNSIYSAILAANLQDQIKVSTVISISFLSNSSFPPSFSSFSSEAGGFMEPIVNFLAKN 336
           PAM +I +AI +A LQ+ IKVS+ + ++ + N SFPPS  SF ++   F++PI+ F+ + 
Sbjct: 142 PAMRNIRNAISSAGLQNNIKVSSSVDMTLIGN-SFPPSQGSFRNDVRSFIDPIIGFVRRI 201

Query: 337 GSPLLANVYPYFTYIDITRTISLDYALFMQSPALTIRDGNFEYNSLFEAMVDALYVALEK 396
            SPLL N+YPYF+Y    R ISL YALF  +P + ++DG+  Y +LF+AM DA+Y AL +
Sbjct: 202 NSPLLVNIYPYFSYAGNPRDISLPYALF-TAPNVVVQDGSLGYRNLFDAMSDAVYAALSR 261

Query: 397 SGGAEVSIVISESGWPSGGGTAATVENAGTYYRNLISFVRGGTPRRPRRAIETYLFAMFD 456
           +GG  + IV+SESGWPS G  AAT  NA TYY+NLI  V+ G+PRRP + IETYLFAMFD
Sbjct: 262 AGGGSIEIVVSESGWPSAGAFAATTNNAATYYKNLIQHVKRGSPRRPNKVIETYLFAMFD 321

Query: 457 ENNKAPEMEKHFGLFTADKKSKYQLRF 480
           ENNK PE+EKHFGLF+ +K+ KY L F
Sbjct: 322 ENNKNPELEKHFGLFSPNKQPKYPLSF 346

BLAST of Spg021905 vs. ExPASy Swiss-Prot
Match: P52407 (Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform OS=Hevea brasiliensis OX=3981 GN=HGN1 PE=1 SV=2)

HSP 1 Score: 350.9 bits (899), Expect = 2.3e-95
Identity = 177/324 (54.63%), Postives = 236/324 (72.84%), Query Frame = 0

Query: 162 VTITES-IGVCYSSSGNNLPDAREVVQLYKSNGIEKMRIYNPDAAMLNALRGSNIEVIVG 221
           V IT++ +GVCY   GNNLP   EV+ LYK + I +MRIY+P+ A+L ALRGSNIE+I+G
Sbjct: 31  VGITDAQVGVCYGMQGNNLPPVSEVIALYKKSNITRMRIYDPNRAVLEALRGSNIELILG 90

Query: 222 IPNTDLEHIVNLSSAMNWVQRNIQAYMADVKFRYIAIGNEVQPSDA----VARYVLPAMN 281
           +PN+DL+ + N S+A +WVQ+N++ + + V FRYIA+GNE+ P +     +A++VLPAM 
Sbjct: 91  VPNSDLQSLTNPSNAKSWVQKNVRGFWSSVLFRYIAVGNEISPVNRGTAWLAQFVLPAMR 150

Query: 282 SIYSAILAANLQDQIKVSTVISISFLSNSSFPPSFSSFSSEAGGFMEPIVNFLAKNGSPL 341
           +I+ AI +A LQDQIKVST I ++ + N S+PPS  +F  +   +++PI+ FL+   SPL
Sbjct: 151 NIHDAIRSAGLQDQIKVSTAIDLTLVGN-SYPPSAGAFRDDVRSYLDPIIGFLSSIRSPL 210

Query: 342 LANVYPYFTYIDITRTISLDYALFMQSPALTIRDGNFEYNSLFEAMVDALYVALEKSGGA 401
           LAN+YPYFTY    R ISL YALF  SP++ + DG   Y +LF+A +DALY ALE++ G 
Sbjct: 211 LANIYPYFTYAYNPRDISLPYALF-TSPSVVVWDGQRGYKNLFDATLDALYSALERASGG 270

Query: 402 EVSIVISESGWPSGGGTAATVENAGTYYRNLISFVRGGTPRRPRRAIETYLFAMFDENNK 461
            + +V+SESGWPS G  AAT +N  TY  NLI  V+GGTP+RP RAIETYLFAMFDEN K
Sbjct: 271 SLEVVVSESGWPSAGAFAATFDNGRTYLSNLIQHVKGGTPKRPNRAIETYLFAMFDENKK 330

Query: 462 APEMEKHFGLFTADKKSKYQLRFS 481
            PE+EKHFGLF  +K  KY L FS
Sbjct: 331 QPEVEKHFGLFFPNKWQKYNLNFS 352

BLAST of Spg021905 vs. ExPASy Swiss-Prot
Match: Q8VZJ2 (Probable glucan endo-1,3-beta-glucosidase At4g16260 OS=Arabidopsis thaliana OX=3702 GN=At4g16260 PE=1 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 1.3e-90
Identity = 165/320 (51.56%), Postives = 227/320 (70.94%), Query Frame = 0

Query: 163 TITESIGVCYSSSGNNLPDAREVVQLYKSNGIEKMRIYNPDAAMLNALRGSNIEVIVGIP 222
           T  ES+GVCY   GNNLP   + + L++ N I ++R+Y+P+ A LNALR + IEVI+G+P
Sbjct: 19  TSGESVGVCYGMMGNNLPSQSDTIALFRQNNIRRVRLYDPNQAALNALRNTGIEVIIGVP 78

Query: 223 NTDLEHIVNLSSAMNWVQRNIQAYMADVKFRYIAIGNEVQPSDAVARYVLPAMNSIYSAI 282
           NTDL  + N SSA +W+Q N+  Y   V F+YIA+GNEV PS+     VLPAM ++Y A+
Sbjct: 79  NTDLRSLTNPSSARSWLQNNVLNYYPAVSFKYIAVGNEVSPSNG-GDVVLPAMRNVYDAL 138

Query: 283 LAANLQDQIKVSTVISISFLSNSSFPPSFSSFSSEAGGFMEPIVNFLAKNGSPLLANVYP 342
             ANLQD+IKVST I ++ + N SFPPS   F  +   +++P++ FL    S LLAN+YP
Sbjct: 139 RGANLQDRIKVSTAIDMTLIGN-SFPPSSGEFRGDVRWYIDPVIGFLTSTNSALLANIYP 198

Query: 343 YFTYIDITRTISLDYALFMQSPALTIRDGNFEYNSLFEAMVDALYVALEKSGGAEVSIVI 402
           YF+Y+D  R ISL YALF  SP++ + DG+  Y +LF+A++D +Y A+E+SGG  + +V+
Sbjct: 199 YFSYVDNPRDISLSYALF-TSPSVVVWDGSRGYQNLFDALLDVVYSAVERSGGGSLPVVV 258

Query: 403 SESGWPSGGGTAATVENAGTYYRNLISFVR--GGTPRRPRRAIETYLFAMFDENNKAPEM 462
           SESGWPS GG AA+ +NA  +Y NL S VR   GTP+RP R +ETYLFAMFDEN K+PE+
Sbjct: 259 SESGWPSNGGNAASFDNARAFYTNLASRVRENRGTPKRPGRGVETYLFAMFDENQKSPEI 318

Query: 463 EKHFGLFTADKKSKYQLRFS 481
           EK+FGLF  +K+ K+ + FS
Sbjct: 319 EKNFGLFFPNKQPKFPITFS 335

BLAST of Spg021905 vs. ExPASy Swiss-Prot
Match: P52408 (Glucan endo-1,3-beta-glucosidase, basic isoform OS=Prunus persica OX=3760 GN=GNS1 PE=3 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 1.3e-90
Identity = 168/314 (53.50%), Postives = 230/314 (73.25%), Query Frame = 0

Query: 168 IGVCYSSSGNNLPDAREVVQLYKSNGIEKMRIYNPDAAMLNALRGSNIEVIVGIPNTDLE 227
           IGVC    G++LP   EVV LYK+N I +MR+Y+P+ A L ALRGSNI++++G+PN +L+
Sbjct: 40  IGVCNGMVGDDLPPQAEVVALYKTNNIPRMRLYDPNPAALEALRGSNIKLLLGVPNENLQ 99

Query: 228 HI-VNLSSAMNWVQRNIQAYMADVKFRYIAIGNEVQPSDAVARYVLPAMNSIYSAILAAN 287
           +I ++ ++A  WVQ N++ Y A+VKF+YIA+GNEV+PSD+ A++++PAM +I  AI  A 
Sbjct: 100 YIALSQANANAWVQNNVRNY-ANVKFKYIAVGNEVKPSDSFAQFLVPAMRNIQEAISLAG 159

Query: 288 LQDQIKVSTVISISFLSNSSFPPSFSSFSSEAGGFMEPIVNFLAKNGSPLLANVYPYFTY 347
           L  +IKVST I    L   +FPPS  SF SE    + PI+ FL  + SPLL N+YPYF Y
Sbjct: 160 LAKKIKVSTAIDTGVL-GETFPPSIGSFKSEYNALLYPIIRFLVSHQSPLLVNLYPYFAY 219

Query: 348 IDITRTISLDYALFMQSPALTIRDGNFEYNSLFEAMVDALYVALEKSGGAEVSIVISESG 407
              T+ I LDYALF  +P++ ++DGNF Y +LF+AM+D +Y ALEK+GG  + +VISE+G
Sbjct: 220 SGNTQDIRLDYALF-TAPSVVVQDGNFGYRNLFDAMLDGVYAALEKAGGGSLKVVISETG 279

Query: 408 WPSGGGTAATVENAGTYYRNLISFVRGGTPRRPRRAIETYLFAMFDENNKAPEMEKHFGL 467
           WPS  GTA T++NA T+  NLI  V+ GTPRRP R IETY+FAMFDEN K PE+EKH+GL
Sbjct: 280 WPSAAGTATTIDNARTFISNLIQHVKEGTPRRPGRPIETYIFAMFDENRKTPELEKHWGL 339

Query: 468 FTADKKSKYQLRFS 481
           F+  K+ KYQ+ F+
Sbjct: 340 FSPTKQPKYQISFN 350

BLAST of Spg021905 vs. ExPASy TrEMBL
Match: A0A6J1DLI3 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111021653 PE=3 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 2.7e-160
Identity = 317/480 (66.04%), Postives = 347/480 (72.29%), Query Frame = 0

Query: 1   MASSIFSRFCSVSVLLLGVLISTGFHQVLGNFPLISLFSIEEPIVDWLDFSRVFNRIVRI 60
           MASSIFS+FCSVS+LLLG LIS GF                          R+F      
Sbjct: 1   MASSIFSKFCSVSLLLLGALISAGF--------------------------RIF------ 60

Query: 61  LNRYGEAGSKLGNMAMAIEDEDEDSMALLPFGSSSGIGSFVRLPQCGGPRRTGTLSPHLF 120
                  G K GNMAMA+  E+ DSMALLPFGS+SGI SFVRLPQCGGPR          
Sbjct: 61  -------GLKSGNMAMALAIEEVDSMALLPFGSASGIDSFVRLPQCGGPR---------- 120

Query: 121 LLRFPFLWMHFCKKKKKNFAFISSSLEKLFDKRYFCLSTRMVTITESIGVCYSSSGNNLP 180
                                                     T+ ES+GVCY+SSG+NLP
Sbjct: 121 -----------------------------------------PTVAESVGVCYASSGSNLP 180

Query: 181 DAREVVQLYKSNGIEKMRIYNPDAAMLNALRGSNIEVIVGIPNTDLEHIVNLSSAMNWVQ 240
            A EVVQLYK+N I KMRIYNP  A+LNAL+GSNIEV+VGIPNTDLEH+VNLSSA NWVQ
Sbjct: 181 SATEVVQLYKNNSIGKMRIYNPSEAILNALQGSNIEVVVGIPNTDLEHVVNLSSAANWVQ 240

Query: 241 RNIQAYMADVKFRYIAIGNEVQPSDAVARYVLPAMNSIYSAILAANLQDQIKVSTVISIS 300
           RNIQAY++ VKFRYIAIGN+V PSD+ A YVLPA+ SIY AI AANLQDQIKVSTVISIS
Sbjct: 241 RNIQAYISHVKFRYIAIGNDVHPSDSFAGYVLPAIRSIYGAISAANLQDQIKVSTVISIS 300

Query: 301 FLSNSSFPPSFSSFSSEAGGFMEPIVNFLAKNGSPLLANVYPYFTYIDITRTISLDYALF 360
            LSNSSFPPS  SFSSEA GF+EPIV+FLA+NGSPLLANVYPYFTY DI RTISLDYALF
Sbjct: 301 LLSNSSFPPSIGSFSSEARGFIEPIVSFLAENGSPLLANVYPYFTYTDIARTISLDYALF 360

Query: 361 MQSPALTIRDGNFEYNSLFEAMVDALYVALEKSGGAEVSIVISESGWPSGGGTAATVENA 420
           MQSPAL IRDGNF YNSLFE+MVDALY ALEKSGG EVSIVISESGWPS  GTAAT+ENA
Sbjct: 361 MQSPALAIRDGNFMYNSLFESMVDALYAALEKSGGNEVSIVISESGWPSSEGTAATIENA 390

Query: 421 GTYYRNLISFVRGGTPRRPRRAIETYLFAMFDENNKAPEMEKHFGLFTADKKSKYQLRFS 480
           GTYYRNLI+FV+ GTPRRP RAIETYLF+MFDEN K PE+EKHFGLFT +KK KYQL FS
Sbjct: 421 GTYYRNLINFVQSGTPRRPGRAIETYLFSMFDENLKPPEIEKHFGLFTPNKKPKYQLSFS 390

BLAST of Spg021905 vs. ExPASy TrEMBL
Match: A0A6J1HBJ9 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461786 PE=3 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 2.7e-160
Identity = 311/430 (72.33%), Postives = 334/430 (77.67%), Query Frame = 0

Query: 51  SRVFNRIVRILNRYGEAGSKLGNMAMAIEDEDEDSMALLPFGSSSGIGSFVRLPQCGGPR 110
           S VF+R +         G K GNM MAI  ED DSMALLPFGS+S IGSF RLPQCGGPR
Sbjct: 15  SLVFHRFI---------GLKSGNMVMAI--EDVDSMALLPFGSASRIGSFFRLPQCGGPR 74

Query: 111 RTGTLSPHLFLLRFPFLWMHFCKKKKKNFAFISSSLEKLFDKRYFCLSTRMVTITESIGV 170
           R                                                     TESIGV
Sbjct: 75  R-----------------------------------------------------TESIGV 134

Query: 171 CYSSSGNNLPDAREVVQLYKSNGIEKMRIYNPDAAMLNALRGSNIEVIVGIPNTDLEHIV 230
           CY++ GNNLPDAREVVQLYK +GIEKMRIYNPD A+LNALRGSNIEVIVGIPNTDLE IV
Sbjct: 135 CYTNLGNNLPDAREVVQLYKIHGIEKMRIYNPDTAILNALRGSNIEVIVGIPNTDLERIV 194

Query: 231 NLSSAMNWVQRNIQAYMADVKFRYIAIGNEVQPSDAVARYVLPAMNSIYSAILAANLQDQ 290
           NLSSA NWVQRNIQAY+  +KFRY+A+GN+VQPSD++ARYVLPA++SI SAI AANLQDQ
Sbjct: 195 NLSSASNWVQRNIQAYVPHIKFRYVAVGNDVQPSDSIARYVLPAISSINSAISAANLQDQ 254

Query: 291 IKVSTVISISFLSNSSFPPSFSSFSSEAGGFMEPIVNFLAKNGSPLLANVYPYFTYIDIT 350
           IKVSTVISIS LSNSSFPPS+SSFSSEA GF+EPIV+FLAKNGSPLLANVYPYFTY DIT
Sbjct: 255 IKVSTVISISLLSNSSFPPSYSSFSSEASGFIEPIVHFLAKNGSPLLANVYPYFTYTDIT 314

Query: 351 RTISLDYALFMQSPALTIRDGNFEYNSLFEAMVDALYVALEKSGGAEVSIVISESGWPSG 410
           RTISLDYALFMQSP L IRD NFEYNSLFE+MVDALYVALEKSGGAEVSIVI+ESGWPS 
Sbjct: 315 RTISLDYALFMQSPTLVIRDDNFEYNSLFESMVDALYVALEKSGGAEVSIVIAESGWPSN 374

Query: 411 GGTAATVENAGTYYRNLISFVRGGTPRRPRRAIETYLFAMFDENNKAPEMEKHFGLFTAD 470
           G  AATVENAGTYYRNL++FV  GTPRRPRRAIETYLFAMFDEN K  EMEKHFGLFTAD
Sbjct: 375 GSAAATVENAGTYYRNLMNFVPTGTPRRPRRAIETYLFAMFDENLKPVEMEKHFGLFTAD 380

Query: 471 KKSKYQLRFS 481
           KKSKYQL FS
Sbjct: 435 KKSKYQLSFS 380

BLAST of Spg021905 vs. ExPASy TrEMBL
Match: A0A6J1H9Q0 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461786 PE=3 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 2.8e-149
Identity = 274/320 (85.62%), Postives = 294/320 (91.88%), Query Frame = 0

Query: 161 MVTITESIGVCYSSSGNNLPDAREVVQLYKSNGIEKMRIYNPDAAMLNALRGSNIEVIVG 220
           MV I ESIGVCY++ GNNLPDAREVVQLYK +GIEKMRIYNPD A+LNALRGSNIEVIVG
Sbjct: 1   MVAIVESIGVCYTNLGNNLPDAREVVQLYKIHGIEKMRIYNPDTAILNALRGSNIEVIVG 60

Query: 221 IPNTDLEHIVNLSSAMNWVQRNIQAYMADVKFRYIAIGNEVQPSDAVARYVLPAMNSIYS 280
           IPNTDLE IVNLSSA NWVQRNIQAY+  +KFRY+A+GN+VQPSD++ARYVLPA++SI S
Sbjct: 61  IPNTDLERIVNLSSASNWVQRNIQAYVPHIKFRYVAVGNDVQPSDSIARYVLPAISSINS 120

Query: 281 AILAANLQDQIKVSTVISISFLSNSSFPPSFSSFSSEAGGFMEPIVNFLAKNGSPLLANV 340
           AI AANLQDQIKVSTVISIS LSNSSFPPS+SSFSSEA GF+EPIV+FLAKNGSPLLANV
Sbjct: 121 AISAANLQDQIKVSTVISISLLSNSSFPPSYSSFSSEASGFIEPIVHFLAKNGSPLLANV 180

Query: 341 YPYFTYIDITRTISLDYALFMQSPALTIRDGNFEYNSLFEAMVDALYVALEKSGGAEVSI 400
           YPYFTY DITRTISLDYALFMQSP L IRD NFEYNSLFE+MVDALYVALEKSGGAEVSI
Sbjct: 181 YPYFTYTDITRTISLDYALFMQSPTLVIRDDNFEYNSLFESMVDALYVALEKSGGAEVSI 240

Query: 401 VISESGWPSGGGTAATVENAGTYYRNLISFVRGGTPRRPRRAIETYLFAMFDENNKAPEM 460
           VI+ESGWPS G  AATVENAGTYYRNL++FV  GTPRRPRRAIETYLFAMFDEN K  EM
Sbjct: 241 VIAESGWPSNGSAAATVENAGTYYRNLMNFVPTGTPRRPRRAIETYLFAMFDENLKPVEM 300

Query: 461 EKHFGLFTADKKSKYQLRFS 481
           EKHFGLFTADKKSKYQL FS
Sbjct: 301 EKHFGLFTADKKSKYQLSFS 320

BLAST of Spg021905 vs. ExPASy TrEMBL
Match: A0A6J1DNI9 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111021658 PE=3 SV=1)

HSP 1 Score: 467.2 bits (1201), Expect = 8.1e-128
Identity = 271/484 (55.99%), Postives = 317/484 (65.50%), Query Frame = 0

Query: 1   MASSIFSRFCSVSVLLLGVLISTGFHQVLGNFPLISLFSIEEPIVDWLDFSRVFNRIVRI 60
           MASS+FS FCS+S+LLLG LIS GF                              RI+  
Sbjct: 1   MASSLFSTFCSLSLLLLGALISAGF------------------------------RII-- 60

Query: 61  LNRYGEAGSKLGN--MAMAIEDEDEDSMALLPFGSSSGIGSFVRLPQCGGPRRTGTLSPH 120
                  GSK GN  MAMA+  ++ DSMALLP GS SGIGSF+RLPQCGGPR T      
Sbjct: 61  -------GSKSGNMAMAMAMAIDEVDSMALLPLGSVSGIGSFIRLPQCGGPRPT------ 120

Query: 121 LFLLRFPFLWMHFCKKKKKNFAFISSSLEKLFDKRYFCLSTRMVTITESIGVCYSSSGNN 180
                                                      V   +++GVCYSS G+N
Sbjct: 121 -------------------------------------------VETVQAVGVCYSSMGDN 180

Query: 181 LPDAREVVQLYKSNGIEKMRIYNPDAAMLNALRGSNIEVIVGIPNTDLEHIVNLSSAMNW 240
           LP+  EVVQLYKS GI++MRIY+ + A+LNALRGSNIEV+VGIPN DL+ +VNLSSA +W
Sbjct: 181 LPEPGEVVQLYKSYGIKRMRIYDTNKAILNALRGSNIEVVVGIPNNDLQRVVNLSSAESW 240

Query: 241 VQRNIQAYMADVKFRYIAIGNEVQPSDAVARYVLPAMNSIYSAILAANLQDQIKVSTVIS 300
           VQRNIQAY+  V FRY+ IGNEV+PSD+ A YVLPAM +IYSAI AANLQDQ+KVSTVIS
Sbjct: 241 VQRNIQAYIPHVNFRYVTIGNEVRPSDSFAGYVLPAMRNIYSAISAANLQDQMKVSTVIS 300

Query: 301 ISFLSNSSFPPSFSSFSSEAGGFMEPIVNFLAKNGSPLLANVYPYFTYIDITRTISLDYA 360
            + L  +S+PPS  SF S+A  F+EPIV+FLAKNGSPLLAN+YPY++YI   R I+LDYA
Sbjct: 301 -TVLLGASYPPSAGSFRSDAREFIEPIVSFLAKNGSPLLANLYPYYSYISNNRDITLDYA 360

Query: 361 LFMQSPALTIRDGNFEYNSLFEAMVDALYVALEKSGGAEVSIVISESGWPSGGGTAATVE 420
           L       T+ DGN  Y++L +A VDALY ALEKSGGA  SIVISESGWPSG GT   VE
Sbjct: 361 LLYDYNFPTVVDGNLVYHNLLDATVDALYAALEKSGGANFSIVISESGWPSGAGTNTVVE 395

Query: 421 N--AGTYYRNLISFVRGGTPRRPRRAIETYLFAMFDENNKAPEMEKHFGLFTADKKSKYQ 480
           N  AGTYYRNLI  V+ G+PRRP RAIETYLF+MFDEN +  E EKHFGLFT D K KYQ
Sbjct: 421 NEVAGTYYRNLIRHVQSGSPRRPGRAIETYLFSMFDENLRPSETEKHFGLFTHDMKQKYQ 395

BLAST of Spg021905 vs. ExPASy TrEMBL
Match: A0A6J1DNH1 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111021643 PE=3 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 1.7e-122
Identity = 246/426 (57.75%), Postives = 299/426 (70.19%), Query Frame = 0

Query: 57  IVRILNRYGEAGSKLGNMAMAIEDEDEDSMALLPFGSSSGIGSFVRLPQCGGPRRTGTLS 116
           ++ +L  +   GSK GN+A+AI  E+ DSMALLPFGS+SGIGSFVRLPQCGGPR T    
Sbjct: 18  LLLLLGAFISPGSKSGNVALAI--EEVDSMALLPFGSASGIGSFVRLPQCGGPRPT---- 77

Query: 117 PHLFLLRFPFLWMHFCKKKKKNFAFISSSLEKLFDKRYFCLSTRMVTITESIGVCYSSSG 176
                                                        V   ++IGVCYS+ G
Sbjct: 78  ---------------------------------------------VETVQAIGVCYSTKG 137

Query: 177 NNLPDAREVVQLYKSNGIEKMRIYNPDAAMLNALRGSNIEVIVGIPNTDLEHIVNLSSAM 236
           +NLP+  EVV+LYKS GI++MRIY+ D  +LNALRGSNIEV+VGIPNTDLE + N+SSA 
Sbjct: 138 DNLPEPGEVVRLYKSLGIKRMRIYDTDQTILNALRGSNIEVVVGIPNTDLERVANVSSAA 197

Query: 237 NWVQRNIQAYMADVKFRYIAIGNEVQPSDAVARYVLPAMNSIYSAILAANLQDQIKVSTV 296
           NWVQ NIQA++  VKFRYI +GNEVQPSD+ A YVLPAM +IYSAI AANLQDQ+K+STV
Sbjct: 198 NWVQMNIQAFVPHVKFRYITVGNEVQPSDSFAGYVLPAMRNIYSAISAANLQDQMKISTV 257

Query: 297 ISISFLSNSSFPPSFSSFSSEAGGFMEPIVNFLAKNGSPLLANVYPYFTYIDITRTISLD 356
           IS + L  +S PPS  SF S+A  F+EPIV+FLAK GSPLLAN+Y Y++YI ++R ++LD
Sbjct: 258 ISTALL-GTSHPPSAGSFGSDAREFIEPIVSFLAKKGSPLLANLYTYYSYIGLSRQMNLD 317

Query: 357 YALFMQSPALTIRDGNFEYNSLFEAMVDALYVALEKSGGAEVSIVISESGWPSGGGTAAT 416
           YAL  +S  + I DGN EY++  E+M+DALY ALE SGGA VSIVISESGWPSG GT   
Sbjct: 318 YALLKESQGV-IADGNLEYHNYVESMIDALYAALENSGGANVSIVISESGWPSGAGTNTI 377

Query: 417 VEN--AGTYYRNLISFVRGGTPRRPRRAIETYLFAMFDENNKAPEMEKHFGLFTADKKSK 476
           +E+  A TYY NLI  V+ GTPRRP RAIETYLF++FDEN +  EMEKHFGLFT D + K
Sbjct: 378 IESVVAETYYVNLIRCVQSGTPRRPGRAIETYLFSIFDENLRPSEMEKHFGLFTHDMEPK 390

Query: 477 YQLRFS 481
           YQ  FS
Sbjct: 438 YQFSFS 390

BLAST of Spg021905 vs. TAIR 10
Match: AT4G16260.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 335.1 bits (858), Expect = 9.2e-92
Identity = 165/320 (51.56%), Postives = 227/320 (70.94%), Query Frame = 0

Query: 163 TITESIGVCYSSSGNNLPDAREVVQLYKSNGIEKMRIYNPDAAMLNALRGSNIEVIVGIP 222
           T  ES+GVCY   GNNLP   + + L++ N I ++R+Y+P+ A LNALR + IEVI+G+P
Sbjct: 19  TSGESVGVCYGMMGNNLPSQSDTIALFRQNNIRRVRLYDPNQAALNALRNTGIEVIIGVP 78

Query: 223 NTDLEHIVNLSSAMNWVQRNIQAYMADVKFRYIAIGNEVQPSDAVARYVLPAMNSIYSAI 282
           NTDL  + N SSA +W+Q N+  Y   V F+YIA+GNEV PS+     VLPAM ++Y A+
Sbjct: 79  NTDLRSLTNPSSARSWLQNNVLNYYPAVSFKYIAVGNEVSPSNG-GDVVLPAMRNVYDAL 138

Query: 283 LAANLQDQIKVSTVISISFLSNSSFPPSFSSFSSEAGGFMEPIVNFLAKNGSPLLANVYP 342
             ANLQD+IKVST I ++ + N SFPPS   F  +   +++P++ FL    S LLAN+YP
Sbjct: 139 RGANLQDRIKVSTAIDMTLIGN-SFPPSSGEFRGDVRWYIDPVIGFLTSTNSALLANIYP 198

Query: 343 YFTYIDITRTISLDYALFMQSPALTIRDGNFEYNSLFEAMVDALYVALEKSGGAEVSIVI 402
           YF+Y+D  R ISL YALF  SP++ + DG+  Y +LF+A++D +Y A+E+SGG  + +V+
Sbjct: 199 YFSYVDNPRDISLSYALF-TSPSVVVWDGSRGYQNLFDALLDVVYSAVERSGGGSLPVVV 258

Query: 403 SESGWPSGGGTAATVENAGTYYRNLISFVR--GGTPRRPRRAIETYLFAMFDENNKAPEM 462
           SESGWPS GG AA+ +NA  +Y NL S VR   GTP+RP R +ETYLFAMFDEN K+PE+
Sbjct: 259 SESGWPSNGGNAASFDNARAFYTNLASRVRENRGTPKRPGRGVETYLFAMFDENQKSPEI 318

Query: 463 EKHFGLFTADKKSKYQLRFS 481
           EK+FGLF  +K+ K+ + FS
Sbjct: 319 EKNFGLFFPNKQPKFPITFS 335

BLAST of Spg021905 vs. TAIR 10
Match: AT3G57240.1 (beta-1,3-glucanase 3 )

HSP 1 Score: 299.3 bits (765), Expect = 5.6e-81
Identity = 165/348 (47.41%), Postives = 225/348 (64.66%), Query Frame = 0

Query: 135 KKKNFAFISSSLEKLFDKRYFCLSTRMVTITESIGVCYSSSGNNLPDAREVVQLYKSNGI 194
           K  N +   +SL  L     F L++   T    IGVCY  +GNNL  A EVV LY+   I
Sbjct: 2   KMCNGSSFLASLPLLLLLLSFILASFFDTAVGQIGVCYGRNGNNLRPASEVVALYQQRNI 61

Query: 195 EKMRIYNPDAAMLNALRGSNIEVIVGIPNTDLEHIV-NLSSAMNWVQRNIQAYMADVKFR 254
            +MR+Y+P+   LNALRGSNIE+++ +PN DL+ +  + + A  WV+ N++ Y A+V FR
Sbjct: 62  RRMRLYDPNQETLNALRGSNIELVLDVPNPDLQRLASSQAEADTWVRNNVRNY-ANVTFR 121

Query: 255 YIAIGNEVQPSDAVARYVLPAMNSIYSAILAANLQDQIKVSTVISISFLSNSSFPPSFSS 314
           YI++GNEVQPSD  A +VLPAM +I  A+ +      IKVST I    +  S FPPS  +
Sbjct: 122 YISVGNEVQPSDQAASFVLPAMQNIERAVSSLG----IKVSTAIDTRGI--SGFPPSSGT 181

Query: 315 FSSEAGGFMEPIVNFLAKNGSPLLANVYPYFTYIDITRTISLDYALFMQSPALTIRDGNF 374
           F+ E   F+ P+++FL+   SPLL N YPYF+Y    R I LDY LF  +P+  + DG  
Sbjct: 182 FTPEFRSFIAPVISFLSSKQSPLLVNNYPYFSYTGNMRDIRLDYTLF-TAPSTVVNDGQN 241

Query: 375 EYNSLFEAMVDALYVALEKSGGAEVSIVISESGWPSGGGTAATVENAGTYYRNLISFVRG 434
           +Y +LF A++D +Y +LEK+GG  + IV+SESGWP+ GG A  V+NA TY  NLI  V+ 
Sbjct: 242 QYRNLFHAILDTVYASLEKAGGGSLEIVVSESGWPTAGGAATGVDNARTYVNNLIQTVKN 301

Query: 435 GTPRRPRRAIETYLFAMFDENNK-APEMEKHFGLFTADKKSKYQLRFS 481
           G+PRRP RA ETY+FAMFDEN+K  PE EK +GLF  + + KY + F+
Sbjct: 302 GSPRRPGRATETYIFAMFDENSKQGPETEKFWGLFLPNLQPKYVVNFN 341

BLAST of Spg021905 vs. TAIR 10
Match: AT3G57270.1 (beta-1,3-glucanase 1 )

HSP 1 Score: 296.2 bits (757), Expect = 4.7e-80
Identity = 154/315 (48.89%), Postives = 213/315 (67.62%), Query Frame = 0

Query: 168 IGVCYSSSGNNLPDAREVVQLYKSNGIEKMRIYNPDAAMLNALRGSNIEVIVGIPNTDLE 227
           +GVCY  +GNNLP   E + L+K   I+++R+Y+PD  +L ALRGSNIEV +G+PN+ L+
Sbjct: 27  VGVCYGRNGNNLPSPAETIALFKQKNIQRVRLYSPDHDVLAALRGSNIEVTLGLPNSYLQ 86

Query: 228 HIVNLSSAMN-WVQRNIQAYMADVKFRYIAIGNEVQPSDAVARYVLPAMNSIYSAILAAN 287
            + +  S  N WVQ  +  Y   V+FRYI++GNEV+ SD+ A++++PAM +I  A+LAA 
Sbjct: 87  SVASSQSQANAWVQTYVMNYANGVRFRYISVGNEVKISDSYAQFLVPAMENIDRAVLAAG 146

Query: 288 LQDQIKVSTVISISFLSNSSFPPSFSSFSSEAGGFMEPIVNFLAKNGSPLLANVYPYFTY 347
           L  +IKVST + +  L   S+PPS  SF  +    MEPI+ FL    SPLL N+Y YF+Y
Sbjct: 147 LGGRIKVSTSVDMGVL-RESYPPSKGSFRGDVMVVMEPIIRFLVSKNSPLLLNLYTYFSY 206

Query: 348 IDITRTISLDYALFMQSPALTIRDGNFEYNSLFEAMVDALYVALEKSGGAEVSIVISESG 407
                 I LDYALF  +P+  + D    Y +LF+AM+DA+Y ALEKSGGA + IV++E+G
Sbjct: 207 AGNVGQIRLDYALF-TAPSGIVSDPPRSYQNLFDAMLDAMYSALEKSGGASLEIVVAETG 266

Query: 408 WPSGGGTAATVENAGTYYRNLISFVRGGTPRRPRRAIETYLFAMFDENNK--APEMEKHF 467
           WP+GGGT   +ENA  Y  NLI  V+ GTP+RP + IETYLFA++DEN K   P +EK +
Sbjct: 267 WPTGGGTDTNIENARIYNNNLIKHVKNGTPKRPGKEIETYLFAIYDENQKPTPPYVEKFW 326

Query: 468 GLFTADKKSKYQLRF 480
           GLF  +K+ KY + F
Sbjct: 327 GLFYPNKQPKYDINF 339

BLAST of Spg021905 vs. TAIR 10
Match: AT3G57260.1 (beta-1,3-glucanase 2 )

HSP 1 Score: 295.8 bits (756), Expect = 6.2e-80
Identity = 154/314 (49.04%), Postives = 212/314 (67.52%), Query Frame = 0

Query: 168 IGVCYSSSGNNLPDAREVVQLYKSNGIEKMRIYNPDAAMLNALRGSNIEVIVGIPNTDLE 227
           IGVCY   G+ LP   +VV LYK   I++MR+Y PD   L ALRGS+IE+I+ +P++DLE
Sbjct: 32  IGVCYGMLGDTLPSPSDVVALYKQQNIQRMRLYGPDPGALAALRGSDIELILDVPSSDLE 91

Query: 228 HIV-NLSSAMNWVQRNIQAYMADVKFRYIAIGNEVQPSDAVARYVLPAMNSIYSAILAAN 287
            +  + + A  WVQ N+Q+Y   V+FRYI +GNEV+PS  V  ++L AM +I +A+  A 
Sbjct: 92  RLASSQTEADKWVQENVQSYRDGVRFRYINVGNEVKPS--VGGFLLQAMQNIENAVSGAG 151

Query: 288 LQDQIKVSTVISISFLSNSSFPPSFSSFSSEAGGFMEPIVNFLAKNGSPLLANVYPYFTY 347
           L  ++KVST I+    +++S PPS   F  E   F+EP++ FLA   SPLL N+YPYF+Y
Sbjct: 152 L--EVKVSTAIATDTTTDTS-PPSQGRFRDEYKSFLEPVIGFLASKQSPLLVNLYPYFSY 211

Query: 348 IDITRTISLDYALFMQSPALTIRDGNFEYNSLFEAMVDALYVALEKSGGAEVSIVISESG 407
           +  T  I LDYALF     +   D  + Y +LF+A +D++Y ALEKSGG  + IV+SE+G
Sbjct: 212 MGDTANIHLDYALFTAQSTVD-NDPGYSYQNLFDANLDSVYAALEKSGGGSLEIVVSETG 271

Query: 408 WPSGGGTAATVENAGTYYRNLISFVRGGTPRRPRRAIETYLFAMFDENNKAPEMEKHFGL 467
           WP+ G    +VENA TY  NLI  V+ G+PRRP +AIETY+FAMFDEN K P  EK +GL
Sbjct: 272 WPTEGAVGTSVENAKTYVNNLIQHVKNGSPRRPGKAIETYIFAMFDENKKEPTYEKFWGL 331

Query: 468 FTADKKSKYQLRFS 481
           F  D++SKY++ F+
Sbjct: 332 FHPDRQSKYEVNFN 339

BLAST of Spg021905 vs. TAIR 10
Match: AT5G20330.1 (beta-1,3-glucanase 4 )

HSP 1 Score: 224.6 bits (571), Expect = 1.8e-58
Identity = 134/348 (38.51%), Postives = 206/348 (59.20%), Query Frame = 0

Query: 136 KKNFAFISSSLEKLFDKRYFCLSTRMVTITESIGVCYSSSGNNLPDAREVVQLYKSNGIE 195
           KK F F  S +    +      ++  VT   SIG+ Y   G+NLP    V+ LYKS GI 
Sbjct: 6   KKLFLFFLSCIVLYVNSN----NSGFVTAANSIGLNYGLLGDNLPSPSNVINLYKSIGIS 65

Query: 196 KMRIYNPDAAMLNALRG-SNIEVIVGIPNTDLEHIVNLSSAM-NWVQRNIQAYMADVKFR 255
           ++RI++P+  +LNALRG  +IEV VG+ + DL  +     A+  W   NI++Y+ADV   
Sbjct: 66  RIRIFDPNTEVLNALRGHRDIEVTVGVKDQDLAALAASEEAVKGWFAANIESYLADVNIT 125

Query: 256 YIAIGNEVQPSDAVARYVLPAMNSIYSAILAANLQDQIKVSTVISISFLSNSSFPPSFSS 315
           +I +GNEV P   +   VLP M S+ + + + NL   I +STV+++S L   S+PPS   
Sbjct: 126 FITVGNEVIPG-PIGPQVLPVMQSLTNLVKSRNL--PISISTVVAMSNL-EQSYPPSAGM 185

Query: 316 FSSEAGGFMEPIVNFLAKNGSPLLANVYPYFTYIDITRTISLDYALFMQSPALTIRDGNF 375
           F+S+A   + P++  L++  +P+L N+YPYF Y      I LDYA F  + ++ ++DG+ 
Sbjct: 186 FTSQAREQLVPVLKLLSQTSTPILVNIYPYFAYASDPANIRLDYASF-NTKSIVVQDGSL 245

Query: 376 EYNSLFEAMVDALYVALEKSGGAEVSIVISESGWPS-GGGTAATVENAGTYYRNLISFVR 435
            Y+++F+A+ DA   A+EK G   + +V+SE+GWPS G G   T   A TY RN +  + 
Sbjct: 246 GYSNMFDAIFDAFVWAMEKEGVKNLPMVVSETGWPSAGNGNFTTPAIASTYNRNFVKHIA 305

Query: 436 G--GTPRRPRRAIETYLFAMFDENNKAPEMEKHFGLFT-ADKKSKYQL 478
              GTP+RP +++  +LFA F+EN K    E++FGL+  +D K  Y+L
Sbjct: 306 SGKGTPKRPNKSMNGFLFATFNENQKPAGTEQNFGLYNPSDMKPIYKL 344

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004139045.13.6e-16267.49glucan endo-1,3-beta-glucosidase [Cucumis sativus] >KAE8653516.1 hypothetical pr... [more]
XP_022961205.15.7e-16072.33glucan endo-1,3-beta-glucosidase-like isoform X1 [Cucurbita moschata][more]
XP_022154372.15.7e-16066.04glucan endo-1,3-beta-glucosidase-like [Momordica charantia][more]
XP_038880622.19.7e-16071.46glucan endo-1,3-beta-glucosidase-like [Benincasa hispida][more]
KAG6590190.11.7e-15974.09Lichenase, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A7PQW31.8e-10058.84Glucan endo-1,3-beta-glucosidase OS=Vitis vinifera OX=29760 GN=VIT_06s0061g00120... [more]
P079795.4e-9754.43Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3[more]
P524072.3e-9554.63Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform OS=Hevea brasiliensis O... [more]
Q8VZJ21.3e-9051.56Probable glucan endo-1,3-beta-glucosidase At4g16260 OS=Arabidopsis thaliana OX=3... [more]
P524081.3e-9053.50Glucan endo-1,3-beta-glucosidase, basic isoform OS=Prunus persica OX=3760 GN=GNS... [more]
Match NameE-valueIdentityDescription
A0A6J1DLI32.7e-16066.04(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111021653 ... [more]
A0A6J1HBJ92.7e-16072.33(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461786 P... [more]
A0A6J1H9Q02.8e-14985.63(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461786 P... [more]
A0A6J1DNI98.1e-12855.99(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111021658 ... [more]
A0A6J1DNH11.7e-12257.75(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111021643 ... [more]
Match NameE-valueIdentityDescription
AT4G16260.19.2e-9251.56Glycosyl hydrolase superfamily protein [more]
AT3G57240.15.6e-8147.41beta-1,3-glucanase 3 [more]
AT3G57270.14.7e-8048.89beta-1,3-glucanase 1 [more]
AT3G57260.16.2e-8049.04beta-1,3-glucanase 2 [more]
AT5G20330.11.8e-5838.51beta-1,3-glucanase 4 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 168..479
e-value: 7.8E-108
score: 360.8
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 398..411
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 166..480
e-value: 2.1E-123
score: 413.3
NoneNo IPR availablePANTHERPTHR32227:SF267GLUCAN ENDO-1,3-BETA-GLUCOSIDASEcoord: 160..477
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 160..477
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 168..477

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg021905.1Spg021905.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0006749 glutathione metabolic process
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds