Homology
BLAST of Spg021905 vs. NCBI nr
Match:
XP_004139045.1 (glucan endo-1,3-beta-glucosidase [Cucumis sativus] >KAE8653516.1 hypothetical protein Csa_006933 [Cucumis sativus])
HSP 1 Score: 582.4 bits (1500), Expect = 3.6e-162
Identity = 326/483 (67.49%), Postives = 352/483 (72.88%), Query Frame = 0
Query: 1 MASS--IFSRFCSVSVLLLGVLISTGFHQVLGNFPLISLFSIEEPIVDWLDFSRVFNRIV 60
MASS FS F V VLLLGVLIS G FNR+V
Sbjct: 1 MASSSIFFSSFSFVLVLLLGVLISPG-----------------------------FNRLV 60
Query: 61 RILNRYGEAGSKLGNMAMAIEDEDEDSMALLPF-GSSSGIGSFVRLPQCGGPRRTGTLSP 120
G KLGNMAMA+ EDEDSMALLPF GS+S +G F+RLPQCGGPRR
Sbjct: 61 ---------GLKLGNMAMAMTIEDEDSMALLPFGGSASSVGRFIRLPQCGGPRR------ 120
Query: 121 HLFLLRFPFLWMHFCKKKKKNFAFISSSLEKLFDKRYFCLSTRMVTITESIGVCYSSSGN 180
TESIGVCYSS+GN
Sbjct: 121 -----------------------------------------------TESIGVCYSSAGN 180
Query: 181 NLPDAREVVQLYKSNGIEKMRIYNPDAAMLNALRGSNIEVIVGIPNTDLEHIVNLSSAMN 240
NLPDAREVVQLYK+N I+KMRIYNPD +LNALRGSNIEVIVGIPNT +EHIVNLSSA N
Sbjct: 181 NLPDAREVVQLYKNNSIKKMRIYNPDTTILNALRGSNIEVIVGIPNTYIEHIVNLSSAAN 240
Query: 241 WVQRNIQAYMADVKFRYIAIGNEVQPSDAVARYVLPAMNSIYSAILAANLQDQIKVSTVI 300
WVQ+NIQAY+ V FRYIA+GNEV+P DA+A+YVLPAM+SIYSAI AANLQDQIKVSTVI
Sbjct: 241 WVQKNIQAYVPYVNFRYIAVGNEVKPPDAIAQYVLPAMSSIYSAISAANLQDQIKVSTVI 300
Query: 301 SISFLSNSSFPPSFSSFSSEAGGFMEPIVNFLAKNGSPLLANVYPYFTYIDITRTISLDY 360
S+SFLSNSSFPPS SFSSEAGGFMEPI+ FLAKNGSPLLANVYPYF Y +I +TISLDY
Sbjct: 301 SMSFLSNSSFPPSNGSFSSEAGGFMEPIIKFLAKNGSPLLANVYPYFVYTEINQTISLDY 360
Query: 361 ALFMQSPALTIRDGNFEYNSLFEAMVDALYVALEKSGGAEVSIVISESGWPSGGGTAATV 420
ALFMQSPAL I DGNF+YNSLFE+MVDALYVALEK GGA+VSIVISESGWPSGGG AT+
Sbjct: 361 ALFMQSPALVISDGNFKYNSLFESMVDALYVALEKLGGAKVSIVISESGWPSGGGNGATI 392
Query: 421 ENAGTYYRNLISFVRGGTPRRPRRAIETYLFAMFDENNKAPEMEKHFGLFTADKKSKYQL 480
ENAGTYYRNLISFVR GTPRRPRRAIETYLFAMFDEN K+ EMEKHFGLFT DKKSKYQL
Sbjct: 421 ENAGTYYRNLISFVRNGTPRRPRRAIETYLFAMFDENLKSLEMEKHFGLFTPDKKSKYQL 392
BLAST of Spg021905 vs. NCBI nr
Match:
XP_022961205.1 (glucan endo-1,3-beta-glucosidase-like isoform X1 [Cucurbita moschata])
HSP 1 Score: 575.1 bits (1481), Expect = 5.7e-160
Identity = 311/430 (72.33%), Postives = 334/430 (77.67%), Query Frame = 0
Query: 51 SRVFNRIVRILNRYGEAGSKLGNMAMAIEDEDEDSMALLPFGSSSGIGSFVRLPQCGGPR 110
S VF+R + G K GNM MAI ED DSMALLPFGS+S IGSF RLPQCGGPR
Sbjct: 15 SLVFHRFI---------GLKSGNMVMAI--EDVDSMALLPFGSASRIGSFFRLPQCGGPR 74
Query: 111 RTGTLSPHLFLLRFPFLWMHFCKKKKKNFAFISSSLEKLFDKRYFCLSTRMVTITESIGV 170
R TESIGV
Sbjct: 75 R-----------------------------------------------------TESIGV 134
Query: 171 CYSSSGNNLPDAREVVQLYKSNGIEKMRIYNPDAAMLNALRGSNIEVIVGIPNTDLEHIV 230
CY++ GNNLPDAREVVQLYK +GIEKMRIYNPD A+LNALRGSNIEVIVGIPNTDLE IV
Sbjct: 135 CYTNLGNNLPDAREVVQLYKIHGIEKMRIYNPDTAILNALRGSNIEVIVGIPNTDLERIV 194
Query: 231 NLSSAMNWVQRNIQAYMADVKFRYIAIGNEVQPSDAVARYVLPAMNSIYSAILAANLQDQ 290
NLSSA NWVQRNIQAY+ +KFRY+A+GN+VQPSD++ARYVLPA++SI SAI AANLQDQ
Sbjct: 195 NLSSASNWVQRNIQAYVPHIKFRYVAVGNDVQPSDSIARYVLPAISSINSAISAANLQDQ 254
Query: 291 IKVSTVISISFLSNSSFPPSFSSFSSEAGGFMEPIVNFLAKNGSPLLANVYPYFTYIDIT 350
IKVSTVISIS LSNSSFPPS+SSFSSEA GF+EPIV+FLAKNGSPLLANVYPYFTY DIT
Sbjct: 255 IKVSTVISISLLSNSSFPPSYSSFSSEASGFIEPIVHFLAKNGSPLLANVYPYFTYTDIT 314
Query: 351 RTISLDYALFMQSPALTIRDGNFEYNSLFEAMVDALYVALEKSGGAEVSIVISESGWPSG 410
RTISLDYALFMQSP L IRD NFEYNSLFE+MVDALYVALEKSGGAEVSIVI+ESGWPS
Sbjct: 315 RTISLDYALFMQSPTLVIRDDNFEYNSLFESMVDALYVALEKSGGAEVSIVIAESGWPSN 374
Query: 411 GGTAATVENAGTYYRNLISFVRGGTPRRPRRAIETYLFAMFDENNKAPEMEKHFGLFTAD 470
G AATVENAGTYYRNL++FV GTPRRPRRAIETYLFAMFDEN K EMEKHFGLFTAD
Sbjct: 375 GSAAATVENAGTYYRNLMNFVPTGTPRRPRRAIETYLFAMFDENLKPVEMEKHFGLFTAD 380
Query: 471 KKSKYQLRFS 481
KKSKYQL FS
Sbjct: 435 KKSKYQLSFS 380
BLAST of Spg021905 vs. NCBI nr
Match:
XP_022154372.1 (glucan endo-1,3-beta-glucosidase-like [Momordica charantia])
HSP 1 Score: 575.1 bits (1481), Expect = 5.7e-160
Identity = 317/480 (66.04%), Postives = 347/480 (72.29%), Query Frame = 0
Query: 1 MASSIFSRFCSVSVLLLGVLISTGFHQVLGNFPLISLFSIEEPIVDWLDFSRVFNRIVRI 60
MASSIFS+FCSVS+LLLG LIS GF R+F
Sbjct: 1 MASSIFSKFCSVSLLLLGALISAGF--------------------------RIF------ 60
Query: 61 LNRYGEAGSKLGNMAMAIEDEDEDSMALLPFGSSSGIGSFVRLPQCGGPRRTGTLSPHLF 120
G K GNMAMA+ E+ DSMALLPFGS+SGI SFVRLPQCGGPR
Sbjct: 61 -------GLKSGNMAMALAIEEVDSMALLPFGSASGIDSFVRLPQCGGPR---------- 120
Query: 121 LLRFPFLWMHFCKKKKKNFAFISSSLEKLFDKRYFCLSTRMVTITESIGVCYSSSGNNLP 180
T+ ES+GVCY+SSG+NLP
Sbjct: 121 -----------------------------------------PTVAESVGVCYASSGSNLP 180
Query: 181 DAREVVQLYKSNGIEKMRIYNPDAAMLNALRGSNIEVIVGIPNTDLEHIVNLSSAMNWVQ 240
A EVVQLYK+N I KMRIYNP A+LNAL+GSNIEV+VGIPNTDLEH+VNLSSA NWVQ
Sbjct: 181 SATEVVQLYKNNSIGKMRIYNPSEAILNALQGSNIEVVVGIPNTDLEHVVNLSSAANWVQ 240
Query: 241 RNIQAYMADVKFRYIAIGNEVQPSDAVARYVLPAMNSIYSAILAANLQDQIKVSTVISIS 300
RNIQAY++ VKFRYIAIGN+V PSD+ A YVLPA+ SIY AI AANLQDQIKVSTVISIS
Sbjct: 241 RNIQAYISHVKFRYIAIGNDVHPSDSFAGYVLPAIRSIYGAISAANLQDQIKVSTVISIS 300
Query: 301 FLSNSSFPPSFSSFSSEAGGFMEPIVNFLAKNGSPLLANVYPYFTYIDITRTISLDYALF 360
LSNSSFPPS SFSSEA GF+EPIV+FLA+NGSPLLANVYPYFTY DI RTISLDYALF
Sbjct: 301 LLSNSSFPPSIGSFSSEARGFIEPIVSFLAENGSPLLANVYPYFTYTDIARTISLDYALF 360
Query: 361 MQSPALTIRDGNFEYNSLFEAMVDALYVALEKSGGAEVSIVISESGWPSGGGTAATVENA 420
MQSPAL IRDGNF YNSLFE+MVDALY ALEKSGG EVSIVISESGWPS GTAAT+ENA
Sbjct: 361 MQSPALAIRDGNFMYNSLFESMVDALYAALEKSGGNEVSIVISESGWPSSEGTAATIENA 390
Query: 421 GTYYRNLISFVRGGTPRRPRRAIETYLFAMFDENNKAPEMEKHFGLFTADKKSKYQLRFS 480
GTYYRNLI+FV+ GTPRRP RAIETYLF+MFDEN K PE+EKHFGLFT +KK KYQL FS
Sbjct: 421 GTYYRNLINFVQSGTPRRPGRAIETYLFSMFDENLKPPEIEKHFGLFTPNKKPKYQLSFS 390
BLAST of Spg021905 vs. NCBI nr
Match:
XP_038880622.1 (glucan endo-1,3-beta-glucosidase-like [Benincasa hispida])
HSP 1 Score: 574.3 bits (1479), Expect = 9.7e-160
Identity = 308/431 (71.46%), Postives = 335/431 (77.73%), Query Frame = 0
Query: 50 FSRVFNRIVRILNRYGEAGSKLGNMAMAIEDEDEDSMALLPFGSSSGIGSFVRLPQCGGP 109
FS + V +L + SK GNM MAI EDEDSMALLPFGS+SG GSF+RL QCGGP
Sbjct: 8 FSTFCSVFVLLLGAFIPPESKSGNMVMAI--EDEDSMALLPFGSASGAGSFIRLSQCGGP 67
Query: 110 RRTGTLSPHLFLLRFPFLWMHFCKKKKKNFAFISSSLEKLFDKRYFCLSTRMVTITESIG 169
RR TESIG
Sbjct: 68 RR-----------------------------------------------------TESIG 127
Query: 170 VCYSSSGNNLPDAREVVQLYKSNGIEKMRIYNPDAAMLNALRGSNIEVIVGIPNTDLEHI 229
VCYSS+GNNLPDA+EVVQLYKSN I+KMRIYNPDAA+LNALRGSNIE+ VGIPNTD+EHI
Sbjct: 128 VCYSSAGNNLPDAKEVVQLYKSNDIKKMRIYNPDAAILNALRGSNIELTVGIPNTDIEHI 187
Query: 230 VNLSSAMNWVQRNIQAYMADVKFRYIAIGNEVQPSDAVARYVLPAMNSIYSAILAANLQD 289
VNLSSA NWV++NI AY+ VKFRY+AIGNEVQPSD ++RYVLPAM+SI+SAI AANLQD
Sbjct: 188 VNLSSAANWVEKNILAYVPLVKFRYVAIGNEVQPSDPISRYVLPAMSSIHSAISAANLQD 247
Query: 290 QIKVSTVISISFLSNSSFPPSFSSFSSEAGGFMEPIVNFLAKNGSPLLANVYPYFTYIDI 349
QIK+STVIS SFLSNSSFPPS SFSSEAGGFMEPIV+FL KNGSPLLANVYPYF Y +I
Sbjct: 248 QIKISTVISTSFLSNSSFPPSNGSFSSEAGGFMEPIVDFLVKNGSPLLANVYPYFVYTEI 307
Query: 350 TRTISLDYALFMQSPALTIRDGNFEYNSLFEAMVDALYVALEKSGGAEVSIVISESGWPS 409
TRTISLDYALFMQSP+L IRDGNF YNSLFE+MVDALYVALEK GG +VSIVISESGWPS
Sbjct: 308 TRTISLDYALFMQSPSLVIRDGNFFYNSLFESMVDALYVALEKLGGDKVSIVISESGWPS 367
Query: 410 GGGTAATVENAGTYYRNLISFVRGGTPRRPRRAIETYLFAMFDENNKAPEMEKHFGLFTA 469
GGG ATVENAGTYYRNLISFVR GTPRRPRRAIETYLFAMFDEN K+ EMEKHFGLFT
Sbjct: 368 GGGKGATVENAGTYYRNLISFVRTGTPRRPRRAIETYLFAMFDENLKSLEMEKHFGLFTP 383
Query: 470 DKKSKYQLRFS 481
DKKSK+QL FS
Sbjct: 428 DKKSKFQLSFS 383
BLAST of Spg021905 vs. NCBI nr
Match:
KAG6590190.1 (Lichenase, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 573.5 bits (1477), Expect = 1.7e-159
Identity = 306/413 (74.09%), Postives = 328/413 (79.42%), Query Frame = 0
Query: 68 GSKLGNMAMAIEDEDEDSMALLPFGSSSGIGSFVRLPQCGGPRRTGTLSPHLFLLRFPFL 127
G K GNM MAI ED DSMALLPFGS+S IGSF RLPQCGGPRR
Sbjct: 7 GLKSGNMVMAI--EDVDSMALLPFGSASRIGSFFRLPQCGGPRR---------------- 66
Query: 128 WMHFCKKKKKNFAFISSSLEKLFDKRYFCLSTRMVTITESIGVCYSSSGNNLPDAREVVQ 187
TESIGVCY++ GNNLPDAREVVQ
Sbjct: 67 -------------------------------------TESIGVCYTNLGNNLPDAREVVQ 126
Query: 188 LYKSNGIEKMRIYNPDAAMLNALRGSNIEVIVGIPNTDLEHIVNLSSAMNWVQRNIQAYM 247
LYK +GIEKMRIYNPD A+LNALRG NIEVIVGIPNTDLE IVNLSSA NWVQRNIQAY+
Sbjct: 127 LYKIHGIEKMRIYNPDTAILNALRGLNIEVIVGIPNTDLERIVNLSSASNWVQRNIQAYV 186
Query: 248 ADVKFRYIAIGNEVQPSDAVARYVLPAMNSIYSAILAANLQDQIKVSTVISISFLSNSSF 307
+KFRY+A+GN+VQPSD++ARYVLPA++SI SAI AANLQDQIKVSTVISIS LSNSSF
Sbjct: 187 PHIKFRYVAVGNDVQPSDSIARYVLPAISSINSAISAANLQDQIKVSTVISISLLSNSSF 246
Query: 308 PPSFSSFSSEAGGFMEPIVNFLAKNGSPLLANVYPYFTYIDITRTISLDYALFMQSPALT 367
PPS+SSFSSEA GF+EPIV+FLAKNGSPLLANVYPYFTY DI+RTISLDYALFMQSP L
Sbjct: 247 PPSYSSFSSEASGFIEPIVHFLAKNGSPLLANVYPYFTYTDISRTISLDYALFMQSPTLV 306
Query: 368 IRDGNFEYNSLFEAMVDALYVALEKSGGAEVSIVISESGWPSGGGTAATVENAGTYYRNL 427
IRDGNFEYNSLFE+MVDALYVALEKSGGAEVSIVI+ESGWPS G AATVENAGTYYRNL
Sbjct: 307 IRDGNFEYNSLFESMVDALYVALEKSGGAEVSIVIAESGWPSNGSAAATVENAGTYYRNL 364
Query: 428 ISFVRGGTPRRPRRAIETYLFAMFDENNKAPEMEKHFGLFTADKKSKYQLRFS 481
++FV GTPRRPRRAIETYLFAMFDEN K EMEKHFGLFTADKKSKYQL FS
Sbjct: 367 MNFVPTGTPRRPRRAIETYLFAMFDENLKPVEMEKHFGLFTADKKSKYQLSFS 364
BLAST of Spg021905 vs. ExPASy Swiss-Prot
Match:
A7PQW3 (Glucan endo-1,3-beta-glucosidase OS=Vitis vinifera OX=29760 GN=VIT_06s0061g00120 PE=1 SV=2)
HSP 1 Score: 367.9 bits (943), Expect = 1.8e-100
Identity = 193/328 (58.84%), Postives = 251/328 (76.52%), Query Frame = 0
Query: 161 MVTIT---ESIGVCYSSSGNNLPDAREVVQLYKSNGIEKMRIYNPDAAMLNALRGSNIEV 220
M TIT +SIGVCY +GNNLP A +V+ LYKSNGI MRIY+P++ L ALRGS+IE+
Sbjct: 20 MSTITIGAQSIGVCYGRNGNNLPSASQVINLYKSNGIGSMRIYDPNSDTLQALRGSDIEL 79
Query: 221 IVGIPNTDLEHIV-NLSSAMNWVQRNIQAYMADVKFRYIAIGNEVQPSDA---VARYVLP 280
I+ +PNTDL+ + + S+A WVQ N+ Y ++VKFRYIA+GNEV P+ + A+YVLP
Sbjct: 80 ILDVPNTDLQSLASDASAAATWVQNNVVNYASEVKFRYIAVGNEVLPTGSNAQYAQYVLP 139
Query: 281 AMNSIYSAILAANLQDQIKVSTVISISFLSNSSFPPSFSSFSSEAGGFMEPIVNFLAKNG 340
AM ++ SAI +A LQDQIKVST + S + S+PPS SFS + F+ PI++FLA+NG
Sbjct: 140 AMKNVQSAITSAGLQDQIKVSTA-TFSAVLGKSYPPSEGSFSDDVSSFINPIISFLAENG 199
Query: 341 SPLLANVYPYFTYIDITRTISLDYALFMQSPALTIRDGNFEYNSLFEAMVDALYVALEKS 400
SPLLAN+YPYF+Y T+ I LDYALF S + ++DG+++Y +LF+A++DALY ALEK+
Sbjct: 200 SPLLANIYPYFSYTGDTQNIRLDYALFTAS-GVVVQDGSYQYQNLFDALLDALYAALEKA 259
Query: 401 GGAEVSIVISESGWPSGGGTAATVENAGTYYRNLISFVRGGTPRRPRRAIETYLFAMFDE 460
GG+ + IV+SESGWPS GGTAATV+NA TYY+NLI+ V+GGTPR+ AIETYLFAMFDE
Sbjct: 260 GGSNLKIVVSESGWPSEGGTAATVDNARTYYKNLINHVKGGTPRK-SGAIETYLFAMFDE 319
Query: 461 NNKAP-EMEKHFGLFTADKKSKYQLRFS 481
N K E EKHFGLFT ++SKYQ+ FS
Sbjct: 320 NQKTGLETEKHFGLFTPGQESKYQISFS 344
BLAST of Spg021905 vs. ExPASy Swiss-Prot
Match:
P07979 (Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3)
HSP 1 Score: 356.3 bits (913), Expect = 5.4e-97
Identity = 178/327 (54.43%), Postives = 241/327 (73.70%), Query Frame = 0
Query: 157 LSTRMVTITESIGVCYSSSGNNLPDAREVVQLYKSNGIEKMRIYNPDAAMLNALRGSNIE 216
+S+ + +S+GVCY GNNLP A +VVQLYKS I +MR+Y+P+ A L ALRGSNIE
Sbjct: 22 VSSTEIVGAQSVGVCYGMLGNNLPPASQVVQLYKSKNIRRMRLYDPNQAALQALRGSNIE 81
Query: 217 VIVGIPNTDLEHI-VNLSSAMNWVQRNIQAYMADVKFRYIAIGNEVQP---SDAVARYVL 276
V++G+PN+DL++I N S+A NWVQRN++ + VKFRYIA+GNEV P + ++ RY+L
Sbjct: 82 VMLGVPNSDLQNIAANPSNANNWVQRNVRNFWPAVKFRYIAVGNEVSPVTGTSSLTRYLL 141
Query: 277 PAMNSIYSAILAANLQDQIKVSTVISISFLSNSSFPPSFSSFSSEAGGFMEPIVNFLAKN 336
PAM +I +AI +A LQ+ IKVS+ + ++ + N SFPPS SF ++ F++PI+ F+ +
Sbjct: 142 PAMRNIRNAISSAGLQNNIKVSSSVDMTLIGN-SFPPSQGSFRNDVRSFIDPIIGFVRRI 201
Query: 337 GSPLLANVYPYFTYIDITRTISLDYALFMQSPALTIRDGNFEYNSLFEAMVDALYVALEK 396
SPLL N+YPYF+Y R ISL YALF +P + ++DG+ Y +LF+AM DA+Y AL +
Sbjct: 202 NSPLLVNIYPYFSYAGNPRDISLPYALF-TAPNVVVQDGSLGYRNLFDAMSDAVYAALSR 261
Query: 397 SGGAEVSIVISESGWPSGGGTAATVENAGTYYRNLISFVRGGTPRRPRRAIETYLFAMFD 456
+GG + IV+SESGWPS G AAT NA TYY+NLI V+ G+PRRP + IETYLFAMFD
Sbjct: 262 AGGGSIEIVVSESGWPSAGAFAATTNNAATYYKNLIQHVKRGSPRRPNKVIETYLFAMFD 321
Query: 457 ENNKAPEMEKHFGLFTADKKSKYQLRF 480
ENNK PE+EKHFGLF+ +K+ KY L F
Sbjct: 322 ENNKNPELEKHFGLFSPNKQPKYPLSF 346
BLAST of Spg021905 vs. ExPASy Swiss-Prot
Match:
P52407 (Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform OS=Hevea brasiliensis OX=3981 GN=HGN1 PE=1 SV=2)
HSP 1 Score: 350.9 bits (899), Expect = 2.3e-95
Identity = 177/324 (54.63%), Postives = 236/324 (72.84%), Query Frame = 0
Query: 162 VTITES-IGVCYSSSGNNLPDAREVVQLYKSNGIEKMRIYNPDAAMLNALRGSNIEVIVG 221
V IT++ +GVCY GNNLP EV+ LYK + I +MRIY+P+ A+L ALRGSNIE+I+G
Sbjct: 31 VGITDAQVGVCYGMQGNNLPPVSEVIALYKKSNITRMRIYDPNRAVLEALRGSNIELILG 90
Query: 222 IPNTDLEHIVNLSSAMNWVQRNIQAYMADVKFRYIAIGNEVQPSDA----VARYVLPAMN 281
+PN+DL+ + N S+A +WVQ+N++ + + V FRYIA+GNE+ P + +A++VLPAM
Sbjct: 91 VPNSDLQSLTNPSNAKSWVQKNVRGFWSSVLFRYIAVGNEISPVNRGTAWLAQFVLPAMR 150
Query: 282 SIYSAILAANLQDQIKVSTVISISFLSNSSFPPSFSSFSSEAGGFMEPIVNFLAKNGSPL 341
+I+ AI +A LQDQIKVST I ++ + N S+PPS +F + +++PI+ FL+ SPL
Sbjct: 151 NIHDAIRSAGLQDQIKVSTAIDLTLVGN-SYPPSAGAFRDDVRSYLDPIIGFLSSIRSPL 210
Query: 342 LANVYPYFTYIDITRTISLDYALFMQSPALTIRDGNFEYNSLFEAMVDALYVALEKSGGA 401
LAN+YPYFTY R ISL YALF SP++ + DG Y +LF+A +DALY ALE++ G
Sbjct: 211 LANIYPYFTYAYNPRDISLPYALF-TSPSVVVWDGQRGYKNLFDATLDALYSALERASGG 270
Query: 402 EVSIVISESGWPSGGGTAATVENAGTYYRNLISFVRGGTPRRPRRAIETYLFAMFDENNK 461
+ +V+SESGWPS G AAT +N TY NLI V+GGTP+RP RAIETYLFAMFDEN K
Sbjct: 271 SLEVVVSESGWPSAGAFAATFDNGRTYLSNLIQHVKGGTPKRPNRAIETYLFAMFDENKK 330
Query: 462 APEMEKHFGLFTADKKSKYQLRFS 481
PE+EKHFGLF +K KY L FS
Sbjct: 331 QPEVEKHFGLFFPNKWQKYNLNFS 352
BLAST of Spg021905 vs. ExPASy Swiss-Prot
Match:
Q8VZJ2 (Probable glucan endo-1,3-beta-glucosidase At4g16260 OS=Arabidopsis thaliana OX=3702 GN=At4g16260 PE=1 SV=1)
HSP 1 Score: 335.1 bits (858), Expect = 1.3e-90
Identity = 165/320 (51.56%), Postives = 227/320 (70.94%), Query Frame = 0
Query: 163 TITESIGVCYSSSGNNLPDAREVVQLYKSNGIEKMRIYNPDAAMLNALRGSNIEVIVGIP 222
T ES+GVCY GNNLP + + L++ N I ++R+Y+P+ A LNALR + IEVI+G+P
Sbjct: 19 TSGESVGVCYGMMGNNLPSQSDTIALFRQNNIRRVRLYDPNQAALNALRNTGIEVIIGVP 78
Query: 223 NTDLEHIVNLSSAMNWVQRNIQAYMADVKFRYIAIGNEVQPSDAVARYVLPAMNSIYSAI 282
NTDL + N SSA +W+Q N+ Y V F+YIA+GNEV PS+ VLPAM ++Y A+
Sbjct: 79 NTDLRSLTNPSSARSWLQNNVLNYYPAVSFKYIAVGNEVSPSNG-GDVVLPAMRNVYDAL 138
Query: 283 LAANLQDQIKVSTVISISFLSNSSFPPSFSSFSSEAGGFMEPIVNFLAKNGSPLLANVYP 342
ANLQD+IKVST I ++ + N SFPPS F + +++P++ FL S LLAN+YP
Sbjct: 139 RGANLQDRIKVSTAIDMTLIGN-SFPPSSGEFRGDVRWYIDPVIGFLTSTNSALLANIYP 198
Query: 343 YFTYIDITRTISLDYALFMQSPALTIRDGNFEYNSLFEAMVDALYVALEKSGGAEVSIVI 402
YF+Y+D R ISL YALF SP++ + DG+ Y +LF+A++D +Y A+E+SGG + +V+
Sbjct: 199 YFSYVDNPRDISLSYALF-TSPSVVVWDGSRGYQNLFDALLDVVYSAVERSGGGSLPVVV 258
Query: 403 SESGWPSGGGTAATVENAGTYYRNLISFVR--GGTPRRPRRAIETYLFAMFDENNKAPEM 462
SESGWPS GG AA+ +NA +Y NL S VR GTP+RP R +ETYLFAMFDEN K+PE+
Sbjct: 259 SESGWPSNGGNAASFDNARAFYTNLASRVRENRGTPKRPGRGVETYLFAMFDENQKSPEI 318
Query: 463 EKHFGLFTADKKSKYQLRFS 481
EK+FGLF +K+ K+ + FS
Sbjct: 319 EKNFGLFFPNKQPKFPITFS 335
BLAST of Spg021905 vs. ExPASy Swiss-Prot
Match:
P52408 (Glucan endo-1,3-beta-glucosidase, basic isoform OS=Prunus persica OX=3760 GN=GNS1 PE=3 SV=1)
HSP 1 Score: 335.1 bits (858), Expect = 1.3e-90
Identity = 168/314 (53.50%), Postives = 230/314 (73.25%), Query Frame = 0
Query: 168 IGVCYSSSGNNLPDAREVVQLYKSNGIEKMRIYNPDAAMLNALRGSNIEVIVGIPNTDLE 227
IGVC G++LP EVV LYK+N I +MR+Y+P+ A L ALRGSNI++++G+PN +L+
Sbjct: 40 IGVCNGMVGDDLPPQAEVVALYKTNNIPRMRLYDPNPAALEALRGSNIKLLLGVPNENLQ 99
Query: 228 HI-VNLSSAMNWVQRNIQAYMADVKFRYIAIGNEVQPSDAVARYVLPAMNSIYSAILAAN 287
+I ++ ++A WVQ N++ Y A+VKF+YIA+GNEV+PSD+ A++++PAM +I AI A
Sbjct: 100 YIALSQANANAWVQNNVRNY-ANVKFKYIAVGNEVKPSDSFAQFLVPAMRNIQEAISLAG 159
Query: 288 LQDQIKVSTVISISFLSNSSFPPSFSSFSSEAGGFMEPIVNFLAKNGSPLLANVYPYFTY 347
L +IKVST I L +FPPS SF SE + PI+ FL + SPLL N+YPYF Y
Sbjct: 160 LAKKIKVSTAIDTGVL-GETFPPSIGSFKSEYNALLYPIIRFLVSHQSPLLVNLYPYFAY 219
Query: 348 IDITRTISLDYALFMQSPALTIRDGNFEYNSLFEAMVDALYVALEKSGGAEVSIVISESG 407
T+ I LDYALF +P++ ++DGNF Y +LF+AM+D +Y ALEK+GG + +VISE+G
Sbjct: 220 SGNTQDIRLDYALF-TAPSVVVQDGNFGYRNLFDAMLDGVYAALEKAGGGSLKVVISETG 279
Query: 408 WPSGGGTAATVENAGTYYRNLISFVRGGTPRRPRRAIETYLFAMFDENNKAPEMEKHFGL 467
WPS GTA T++NA T+ NLI V+ GTPRRP R IETY+FAMFDEN K PE+EKH+GL
Sbjct: 280 WPSAAGTATTIDNARTFISNLIQHVKEGTPRRPGRPIETYIFAMFDENRKTPELEKHWGL 339
Query: 468 FTADKKSKYQLRFS 481
F+ K+ KYQ+ F+
Sbjct: 340 FSPTKQPKYQISFN 350
BLAST of Spg021905 vs. ExPASy TrEMBL
Match:
A0A6J1DLI3 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111021653 PE=3 SV=1)
HSP 1 Score: 575.1 bits (1481), Expect = 2.7e-160
Identity = 317/480 (66.04%), Postives = 347/480 (72.29%), Query Frame = 0
Query: 1 MASSIFSRFCSVSVLLLGVLISTGFHQVLGNFPLISLFSIEEPIVDWLDFSRVFNRIVRI 60
MASSIFS+FCSVS+LLLG LIS GF R+F
Sbjct: 1 MASSIFSKFCSVSLLLLGALISAGF--------------------------RIF------ 60
Query: 61 LNRYGEAGSKLGNMAMAIEDEDEDSMALLPFGSSSGIGSFVRLPQCGGPRRTGTLSPHLF 120
G K GNMAMA+ E+ DSMALLPFGS+SGI SFVRLPQCGGPR
Sbjct: 61 -------GLKSGNMAMALAIEEVDSMALLPFGSASGIDSFVRLPQCGGPR---------- 120
Query: 121 LLRFPFLWMHFCKKKKKNFAFISSSLEKLFDKRYFCLSTRMVTITESIGVCYSSSGNNLP 180
T+ ES+GVCY+SSG+NLP
Sbjct: 121 -----------------------------------------PTVAESVGVCYASSGSNLP 180
Query: 181 DAREVVQLYKSNGIEKMRIYNPDAAMLNALRGSNIEVIVGIPNTDLEHIVNLSSAMNWVQ 240
A EVVQLYK+N I KMRIYNP A+LNAL+GSNIEV+VGIPNTDLEH+VNLSSA NWVQ
Sbjct: 181 SATEVVQLYKNNSIGKMRIYNPSEAILNALQGSNIEVVVGIPNTDLEHVVNLSSAANWVQ 240
Query: 241 RNIQAYMADVKFRYIAIGNEVQPSDAVARYVLPAMNSIYSAILAANLQDQIKVSTVISIS 300
RNIQAY++ VKFRYIAIGN+V PSD+ A YVLPA+ SIY AI AANLQDQIKVSTVISIS
Sbjct: 241 RNIQAYISHVKFRYIAIGNDVHPSDSFAGYVLPAIRSIYGAISAANLQDQIKVSTVISIS 300
Query: 301 FLSNSSFPPSFSSFSSEAGGFMEPIVNFLAKNGSPLLANVYPYFTYIDITRTISLDYALF 360
LSNSSFPPS SFSSEA GF+EPIV+FLA+NGSPLLANVYPYFTY DI RTISLDYALF
Sbjct: 301 LLSNSSFPPSIGSFSSEARGFIEPIVSFLAENGSPLLANVYPYFTYTDIARTISLDYALF 360
Query: 361 MQSPALTIRDGNFEYNSLFEAMVDALYVALEKSGGAEVSIVISESGWPSGGGTAATVENA 420
MQSPAL IRDGNF YNSLFE+MVDALY ALEKSGG EVSIVISESGWPS GTAAT+ENA
Sbjct: 361 MQSPALAIRDGNFMYNSLFESMVDALYAALEKSGGNEVSIVISESGWPSSEGTAATIENA 390
Query: 421 GTYYRNLISFVRGGTPRRPRRAIETYLFAMFDENNKAPEMEKHFGLFTADKKSKYQLRFS 480
GTYYRNLI+FV+ GTPRRP RAIETYLF+MFDEN K PE+EKHFGLFT +KK KYQL FS
Sbjct: 421 GTYYRNLINFVQSGTPRRPGRAIETYLFSMFDENLKPPEIEKHFGLFTPNKKPKYQLSFS 390
BLAST of Spg021905 vs. ExPASy TrEMBL
Match:
A0A6J1HBJ9 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461786 PE=3 SV=1)
HSP 1 Score: 575.1 bits (1481), Expect = 2.7e-160
Identity = 311/430 (72.33%), Postives = 334/430 (77.67%), Query Frame = 0
Query: 51 SRVFNRIVRILNRYGEAGSKLGNMAMAIEDEDEDSMALLPFGSSSGIGSFVRLPQCGGPR 110
S VF+R + G K GNM MAI ED DSMALLPFGS+S IGSF RLPQCGGPR
Sbjct: 15 SLVFHRFI---------GLKSGNMVMAI--EDVDSMALLPFGSASRIGSFFRLPQCGGPR 74
Query: 111 RTGTLSPHLFLLRFPFLWMHFCKKKKKNFAFISSSLEKLFDKRYFCLSTRMVTITESIGV 170
R TESIGV
Sbjct: 75 R-----------------------------------------------------TESIGV 134
Query: 171 CYSSSGNNLPDAREVVQLYKSNGIEKMRIYNPDAAMLNALRGSNIEVIVGIPNTDLEHIV 230
CY++ GNNLPDAREVVQLYK +GIEKMRIYNPD A+LNALRGSNIEVIVGIPNTDLE IV
Sbjct: 135 CYTNLGNNLPDAREVVQLYKIHGIEKMRIYNPDTAILNALRGSNIEVIVGIPNTDLERIV 194
Query: 231 NLSSAMNWVQRNIQAYMADVKFRYIAIGNEVQPSDAVARYVLPAMNSIYSAILAANLQDQ 290
NLSSA NWVQRNIQAY+ +KFRY+A+GN+VQPSD++ARYVLPA++SI SAI AANLQDQ
Sbjct: 195 NLSSASNWVQRNIQAYVPHIKFRYVAVGNDVQPSDSIARYVLPAISSINSAISAANLQDQ 254
Query: 291 IKVSTVISISFLSNSSFPPSFSSFSSEAGGFMEPIVNFLAKNGSPLLANVYPYFTYIDIT 350
IKVSTVISIS LSNSSFPPS+SSFSSEA GF+EPIV+FLAKNGSPLLANVYPYFTY DIT
Sbjct: 255 IKVSTVISISLLSNSSFPPSYSSFSSEASGFIEPIVHFLAKNGSPLLANVYPYFTYTDIT 314
Query: 351 RTISLDYALFMQSPALTIRDGNFEYNSLFEAMVDALYVALEKSGGAEVSIVISESGWPSG 410
RTISLDYALFMQSP L IRD NFEYNSLFE+MVDALYVALEKSGGAEVSIVI+ESGWPS
Sbjct: 315 RTISLDYALFMQSPTLVIRDDNFEYNSLFESMVDALYVALEKSGGAEVSIVIAESGWPSN 374
Query: 411 GGTAATVENAGTYYRNLISFVRGGTPRRPRRAIETYLFAMFDENNKAPEMEKHFGLFTAD 470
G AATVENAGTYYRNL++FV GTPRRPRRAIETYLFAMFDEN K EMEKHFGLFTAD
Sbjct: 375 GSAAATVENAGTYYRNLMNFVPTGTPRRPRRAIETYLFAMFDENLKPVEMEKHFGLFTAD 380
Query: 471 KKSKYQLRFS 481
KKSKYQL FS
Sbjct: 435 KKSKYQLSFS 380
BLAST of Spg021905 vs. ExPASy TrEMBL
Match:
A0A6J1H9Q0 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461786 PE=3 SV=1)
HSP 1 Score: 538.5 bits (1386), Expect = 2.8e-149
Identity = 274/320 (85.62%), Postives = 294/320 (91.88%), Query Frame = 0
Query: 161 MVTITESIGVCYSSSGNNLPDAREVVQLYKSNGIEKMRIYNPDAAMLNALRGSNIEVIVG 220
MV I ESIGVCY++ GNNLPDAREVVQLYK +GIEKMRIYNPD A+LNALRGSNIEVIVG
Sbjct: 1 MVAIVESIGVCYTNLGNNLPDAREVVQLYKIHGIEKMRIYNPDTAILNALRGSNIEVIVG 60
Query: 221 IPNTDLEHIVNLSSAMNWVQRNIQAYMADVKFRYIAIGNEVQPSDAVARYVLPAMNSIYS 280
IPNTDLE IVNLSSA NWVQRNIQAY+ +KFRY+A+GN+VQPSD++ARYVLPA++SI S
Sbjct: 61 IPNTDLERIVNLSSASNWVQRNIQAYVPHIKFRYVAVGNDVQPSDSIARYVLPAISSINS 120
Query: 281 AILAANLQDQIKVSTVISISFLSNSSFPPSFSSFSSEAGGFMEPIVNFLAKNGSPLLANV 340
AI AANLQDQIKVSTVISIS LSNSSFPPS+SSFSSEA GF+EPIV+FLAKNGSPLLANV
Sbjct: 121 AISAANLQDQIKVSTVISISLLSNSSFPPSYSSFSSEASGFIEPIVHFLAKNGSPLLANV 180
Query: 341 YPYFTYIDITRTISLDYALFMQSPALTIRDGNFEYNSLFEAMVDALYVALEKSGGAEVSI 400
YPYFTY DITRTISLDYALFMQSP L IRD NFEYNSLFE+MVDALYVALEKSGGAEVSI
Sbjct: 181 YPYFTYTDITRTISLDYALFMQSPTLVIRDDNFEYNSLFESMVDALYVALEKSGGAEVSI 240
Query: 401 VISESGWPSGGGTAATVENAGTYYRNLISFVRGGTPRRPRRAIETYLFAMFDENNKAPEM 460
VI+ESGWPS G AATVENAGTYYRNL++FV GTPRRPRRAIETYLFAMFDEN K EM
Sbjct: 241 VIAESGWPSNGSAAATVENAGTYYRNLMNFVPTGTPRRPRRAIETYLFAMFDENLKPVEM 300
Query: 461 EKHFGLFTADKKSKYQLRFS 481
EKHFGLFTADKKSKYQL FS
Sbjct: 301 EKHFGLFTADKKSKYQLSFS 320
BLAST of Spg021905 vs. ExPASy TrEMBL
Match:
A0A6J1DNI9 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111021658 PE=3 SV=1)
HSP 1 Score: 467.2 bits (1201), Expect = 8.1e-128
Identity = 271/484 (55.99%), Postives = 317/484 (65.50%), Query Frame = 0
Query: 1 MASSIFSRFCSVSVLLLGVLISTGFHQVLGNFPLISLFSIEEPIVDWLDFSRVFNRIVRI 60
MASS+FS FCS+S+LLLG LIS GF RI+
Sbjct: 1 MASSLFSTFCSLSLLLLGALISAGF------------------------------RII-- 60
Query: 61 LNRYGEAGSKLGN--MAMAIEDEDEDSMALLPFGSSSGIGSFVRLPQCGGPRRTGTLSPH 120
GSK GN MAMA+ ++ DSMALLP GS SGIGSF+RLPQCGGPR T
Sbjct: 61 -------GSKSGNMAMAMAMAIDEVDSMALLPLGSVSGIGSFIRLPQCGGPRPT------ 120
Query: 121 LFLLRFPFLWMHFCKKKKKNFAFISSSLEKLFDKRYFCLSTRMVTITESIGVCYSSSGNN 180
V +++GVCYSS G+N
Sbjct: 121 -------------------------------------------VETVQAVGVCYSSMGDN 180
Query: 181 LPDAREVVQLYKSNGIEKMRIYNPDAAMLNALRGSNIEVIVGIPNTDLEHIVNLSSAMNW 240
LP+ EVVQLYKS GI++MRIY+ + A+LNALRGSNIEV+VGIPN DL+ +VNLSSA +W
Sbjct: 181 LPEPGEVVQLYKSYGIKRMRIYDTNKAILNALRGSNIEVVVGIPNNDLQRVVNLSSAESW 240
Query: 241 VQRNIQAYMADVKFRYIAIGNEVQPSDAVARYVLPAMNSIYSAILAANLQDQIKVSTVIS 300
VQRNIQAY+ V FRY+ IGNEV+PSD+ A YVLPAM +IYSAI AANLQDQ+KVSTVIS
Sbjct: 241 VQRNIQAYIPHVNFRYVTIGNEVRPSDSFAGYVLPAMRNIYSAISAANLQDQMKVSTVIS 300
Query: 301 ISFLSNSSFPPSFSSFSSEAGGFMEPIVNFLAKNGSPLLANVYPYFTYIDITRTISLDYA 360
+ L +S+PPS SF S+A F+EPIV+FLAKNGSPLLAN+YPY++YI R I+LDYA
Sbjct: 301 -TVLLGASYPPSAGSFRSDAREFIEPIVSFLAKNGSPLLANLYPYYSYISNNRDITLDYA 360
Query: 361 LFMQSPALTIRDGNFEYNSLFEAMVDALYVALEKSGGAEVSIVISESGWPSGGGTAATVE 420
L T+ DGN Y++L +A VDALY ALEKSGGA SIVISESGWPSG GT VE
Sbjct: 361 LLYDYNFPTVVDGNLVYHNLLDATVDALYAALEKSGGANFSIVISESGWPSGAGTNTVVE 395
Query: 421 N--AGTYYRNLISFVRGGTPRRPRRAIETYLFAMFDENNKAPEMEKHFGLFTADKKSKYQ 480
N AGTYYRNLI V+ G+PRRP RAIETYLF+MFDEN + E EKHFGLFT D K KYQ
Sbjct: 421 NEVAGTYYRNLIRHVQSGSPRRPGRAIETYLFSMFDENLRPSETEKHFGLFTHDMKQKYQ 395
BLAST of Spg021905 vs. ExPASy TrEMBL
Match:
A0A6J1DNH1 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111021643 PE=3 SV=1)
HSP 1 Score: 449.5 bits (1155), Expect = 1.7e-122
Identity = 246/426 (57.75%), Postives = 299/426 (70.19%), Query Frame = 0
Query: 57 IVRILNRYGEAGSKLGNMAMAIEDEDEDSMALLPFGSSSGIGSFVRLPQCGGPRRTGTLS 116
++ +L + GSK GN+A+AI E+ DSMALLPFGS+SGIGSFVRLPQCGGPR T
Sbjct: 18 LLLLLGAFISPGSKSGNVALAI--EEVDSMALLPFGSASGIGSFVRLPQCGGPRPT---- 77
Query: 117 PHLFLLRFPFLWMHFCKKKKKNFAFISSSLEKLFDKRYFCLSTRMVTITESIGVCYSSSG 176
V ++IGVCYS+ G
Sbjct: 78 ---------------------------------------------VETVQAIGVCYSTKG 137
Query: 177 NNLPDAREVVQLYKSNGIEKMRIYNPDAAMLNALRGSNIEVIVGIPNTDLEHIVNLSSAM 236
+NLP+ EVV+LYKS GI++MRIY+ D +LNALRGSNIEV+VGIPNTDLE + N+SSA
Sbjct: 138 DNLPEPGEVVRLYKSLGIKRMRIYDTDQTILNALRGSNIEVVVGIPNTDLERVANVSSAA 197
Query: 237 NWVQRNIQAYMADVKFRYIAIGNEVQPSDAVARYVLPAMNSIYSAILAANLQDQIKVSTV 296
NWVQ NIQA++ VKFRYI +GNEVQPSD+ A YVLPAM +IYSAI AANLQDQ+K+STV
Sbjct: 198 NWVQMNIQAFVPHVKFRYITVGNEVQPSDSFAGYVLPAMRNIYSAISAANLQDQMKISTV 257
Query: 297 ISISFLSNSSFPPSFSSFSSEAGGFMEPIVNFLAKNGSPLLANVYPYFTYIDITRTISLD 356
IS + L +S PPS SF S+A F+EPIV+FLAK GSPLLAN+Y Y++YI ++R ++LD
Sbjct: 258 ISTALL-GTSHPPSAGSFGSDAREFIEPIVSFLAKKGSPLLANLYTYYSYIGLSRQMNLD 317
Query: 357 YALFMQSPALTIRDGNFEYNSLFEAMVDALYVALEKSGGAEVSIVISESGWPSGGGTAAT 416
YAL +S + I DGN EY++ E+M+DALY ALE SGGA VSIVISESGWPSG GT
Sbjct: 318 YALLKESQGV-IADGNLEYHNYVESMIDALYAALENSGGANVSIVISESGWPSGAGTNTI 377
Query: 417 VEN--AGTYYRNLISFVRGGTPRRPRRAIETYLFAMFDENNKAPEMEKHFGLFTADKKSK 476
+E+ A TYY NLI V+ GTPRRP RAIETYLF++FDEN + EMEKHFGLFT D + K
Sbjct: 378 IESVVAETYYVNLIRCVQSGTPRRPGRAIETYLFSIFDENLRPSEMEKHFGLFTHDMEPK 390
Query: 477 YQLRFS 481
YQ FS
Sbjct: 438 YQFSFS 390
BLAST of Spg021905 vs. TAIR 10
Match:
AT4G16260.1 (Glycosyl hydrolase superfamily protein )
HSP 1 Score: 335.1 bits (858), Expect = 9.2e-92
Identity = 165/320 (51.56%), Postives = 227/320 (70.94%), Query Frame = 0
Query: 163 TITESIGVCYSSSGNNLPDAREVVQLYKSNGIEKMRIYNPDAAMLNALRGSNIEVIVGIP 222
T ES+GVCY GNNLP + + L++ N I ++R+Y+P+ A LNALR + IEVI+G+P
Sbjct: 19 TSGESVGVCYGMMGNNLPSQSDTIALFRQNNIRRVRLYDPNQAALNALRNTGIEVIIGVP 78
Query: 223 NTDLEHIVNLSSAMNWVQRNIQAYMADVKFRYIAIGNEVQPSDAVARYVLPAMNSIYSAI 282
NTDL + N SSA +W+Q N+ Y V F+YIA+GNEV PS+ VLPAM ++Y A+
Sbjct: 79 NTDLRSLTNPSSARSWLQNNVLNYYPAVSFKYIAVGNEVSPSNG-GDVVLPAMRNVYDAL 138
Query: 283 LAANLQDQIKVSTVISISFLSNSSFPPSFSSFSSEAGGFMEPIVNFLAKNGSPLLANVYP 342
ANLQD+IKVST I ++ + N SFPPS F + +++P++ FL S LLAN+YP
Sbjct: 139 RGANLQDRIKVSTAIDMTLIGN-SFPPSSGEFRGDVRWYIDPVIGFLTSTNSALLANIYP 198
Query: 343 YFTYIDITRTISLDYALFMQSPALTIRDGNFEYNSLFEAMVDALYVALEKSGGAEVSIVI 402
YF+Y+D R ISL YALF SP++ + DG+ Y +LF+A++D +Y A+E+SGG + +V+
Sbjct: 199 YFSYVDNPRDISLSYALF-TSPSVVVWDGSRGYQNLFDALLDVVYSAVERSGGGSLPVVV 258
Query: 403 SESGWPSGGGTAATVENAGTYYRNLISFVR--GGTPRRPRRAIETYLFAMFDENNKAPEM 462
SESGWPS GG AA+ +NA +Y NL S VR GTP+RP R +ETYLFAMFDEN K+PE+
Sbjct: 259 SESGWPSNGGNAASFDNARAFYTNLASRVRENRGTPKRPGRGVETYLFAMFDENQKSPEI 318
Query: 463 EKHFGLFTADKKSKYQLRFS 481
EK+FGLF +K+ K+ + FS
Sbjct: 319 EKNFGLFFPNKQPKFPITFS 335
BLAST of Spg021905 vs. TAIR 10
Match:
AT3G57240.1 (beta-1,3-glucanase 3 )
HSP 1 Score: 299.3 bits (765), Expect = 5.6e-81
Identity = 165/348 (47.41%), Postives = 225/348 (64.66%), Query Frame = 0
Query: 135 KKKNFAFISSSLEKLFDKRYFCLSTRMVTITESIGVCYSSSGNNLPDAREVVQLYKSNGI 194
K N + +SL L F L++ T IGVCY +GNNL A EVV LY+ I
Sbjct: 2 KMCNGSSFLASLPLLLLLLSFILASFFDTAVGQIGVCYGRNGNNLRPASEVVALYQQRNI 61
Query: 195 EKMRIYNPDAAMLNALRGSNIEVIVGIPNTDLEHIV-NLSSAMNWVQRNIQAYMADVKFR 254
+MR+Y+P+ LNALRGSNIE+++ +PN DL+ + + + A WV+ N++ Y A+V FR
Sbjct: 62 RRMRLYDPNQETLNALRGSNIELVLDVPNPDLQRLASSQAEADTWVRNNVRNY-ANVTFR 121
Query: 255 YIAIGNEVQPSDAVARYVLPAMNSIYSAILAANLQDQIKVSTVISISFLSNSSFPPSFSS 314
YI++GNEVQPSD A +VLPAM +I A+ + IKVST I + S FPPS +
Sbjct: 122 YISVGNEVQPSDQAASFVLPAMQNIERAVSSLG----IKVSTAIDTRGI--SGFPPSSGT 181
Query: 315 FSSEAGGFMEPIVNFLAKNGSPLLANVYPYFTYIDITRTISLDYALFMQSPALTIRDGNF 374
F+ E F+ P+++FL+ SPLL N YPYF+Y R I LDY LF +P+ + DG
Sbjct: 182 FTPEFRSFIAPVISFLSSKQSPLLVNNYPYFSYTGNMRDIRLDYTLF-TAPSTVVNDGQN 241
Query: 375 EYNSLFEAMVDALYVALEKSGGAEVSIVISESGWPSGGGTAATVENAGTYYRNLISFVRG 434
+Y +LF A++D +Y +LEK+GG + IV+SESGWP+ GG A V+NA TY NLI V+
Sbjct: 242 QYRNLFHAILDTVYASLEKAGGGSLEIVVSESGWPTAGGAATGVDNARTYVNNLIQTVKN 301
Query: 435 GTPRRPRRAIETYLFAMFDENNK-APEMEKHFGLFTADKKSKYQLRFS 481
G+PRRP RA ETY+FAMFDEN+K PE EK +GLF + + KY + F+
Sbjct: 302 GSPRRPGRATETYIFAMFDENSKQGPETEKFWGLFLPNLQPKYVVNFN 341
BLAST of Spg021905 vs. TAIR 10
Match:
AT3G57270.1 (beta-1,3-glucanase 1 )
HSP 1 Score: 296.2 bits (757), Expect = 4.7e-80
Identity = 154/315 (48.89%), Postives = 213/315 (67.62%), Query Frame = 0
Query: 168 IGVCYSSSGNNLPDAREVVQLYKSNGIEKMRIYNPDAAMLNALRGSNIEVIVGIPNTDLE 227
+GVCY +GNNLP E + L+K I+++R+Y+PD +L ALRGSNIEV +G+PN+ L+
Sbjct: 27 VGVCYGRNGNNLPSPAETIALFKQKNIQRVRLYSPDHDVLAALRGSNIEVTLGLPNSYLQ 86
Query: 228 HIVNLSSAMN-WVQRNIQAYMADVKFRYIAIGNEVQPSDAVARYVLPAMNSIYSAILAAN 287
+ + S N WVQ + Y V+FRYI++GNEV+ SD+ A++++PAM +I A+LAA
Sbjct: 87 SVASSQSQANAWVQTYVMNYANGVRFRYISVGNEVKISDSYAQFLVPAMENIDRAVLAAG 146
Query: 288 LQDQIKVSTVISISFLSNSSFPPSFSSFSSEAGGFMEPIVNFLAKNGSPLLANVYPYFTY 347
L +IKVST + + L S+PPS SF + MEPI+ FL SPLL N+Y YF+Y
Sbjct: 147 LGGRIKVSTSVDMGVL-RESYPPSKGSFRGDVMVVMEPIIRFLVSKNSPLLLNLYTYFSY 206
Query: 348 IDITRTISLDYALFMQSPALTIRDGNFEYNSLFEAMVDALYVALEKSGGAEVSIVISESG 407
I LDYALF +P+ + D Y +LF+AM+DA+Y ALEKSGGA + IV++E+G
Sbjct: 207 AGNVGQIRLDYALF-TAPSGIVSDPPRSYQNLFDAMLDAMYSALEKSGGASLEIVVAETG 266
Query: 408 WPSGGGTAATVENAGTYYRNLISFVRGGTPRRPRRAIETYLFAMFDENNK--APEMEKHF 467
WP+GGGT +ENA Y NLI V+ GTP+RP + IETYLFA++DEN K P +EK +
Sbjct: 267 WPTGGGTDTNIENARIYNNNLIKHVKNGTPKRPGKEIETYLFAIYDENQKPTPPYVEKFW 326
Query: 468 GLFTADKKSKYQLRF 480
GLF +K+ KY + F
Sbjct: 327 GLFYPNKQPKYDINF 339
BLAST of Spg021905 vs. TAIR 10
Match:
AT3G57260.1 (beta-1,3-glucanase 2 )
HSP 1 Score: 295.8 bits (756), Expect = 6.2e-80
Identity = 154/314 (49.04%), Postives = 212/314 (67.52%), Query Frame = 0
Query: 168 IGVCYSSSGNNLPDAREVVQLYKSNGIEKMRIYNPDAAMLNALRGSNIEVIVGIPNTDLE 227
IGVCY G+ LP +VV LYK I++MR+Y PD L ALRGS+IE+I+ +P++DLE
Sbjct: 32 IGVCYGMLGDTLPSPSDVVALYKQQNIQRMRLYGPDPGALAALRGSDIELILDVPSSDLE 91
Query: 228 HIV-NLSSAMNWVQRNIQAYMADVKFRYIAIGNEVQPSDAVARYVLPAMNSIYSAILAAN 287
+ + + A WVQ N+Q+Y V+FRYI +GNEV+PS V ++L AM +I +A+ A
Sbjct: 92 RLASSQTEADKWVQENVQSYRDGVRFRYINVGNEVKPS--VGGFLLQAMQNIENAVSGAG 151
Query: 288 LQDQIKVSTVISISFLSNSSFPPSFSSFSSEAGGFMEPIVNFLAKNGSPLLANVYPYFTY 347
L ++KVST I+ +++S PPS F E F+EP++ FLA SPLL N+YPYF+Y
Sbjct: 152 L--EVKVSTAIATDTTTDTS-PPSQGRFRDEYKSFLEPVIGFLASKQSPLLVNLYPYFSY 211
Query: 348 IDITRTISLDYALFMQSPALTIRDGNFEYNSLFEAMVDALYVALEKSGGAEVSIVISESG 407
+ T I LDYALF + D + Y +LF+A +D++Y ALEKSGG + IV+SE+G
Sbjct: 212 MGDTANIHLDYALFTAQSTVD-NDPGYSYQNLFDANLDSVYAALEKSGGGSLEIVVSETG 271
Query: 408 WPSGGGTAATVENAGTYYRNLISFVRGGTPRRPRRAIETYLFAMFDENNKAPEMEKHFGL 467
WP+ G +VENA TY NLI V+ G+PRRP +AIETY+FAMFDEN K P EK +GL
Sbjct: 272 WPTEGAVGTSVENAKTYVNNLIQHVKNGSPRRPGKAIETYIFAMFDENKKEPTYEKFWGL 331
Query: 468 FTADKKSKYQLRFS 481
F D++SKY++ F+
Sbjct: 332 FHPDRQSKYEVNFN 339
BLAST of Spg021905 vs. TAIR 10
Match:
AT5G20330.1 (beta-1,3-glucanase 4 )
HSP 1 Score: 224.6 bits (571), Expect = 1.8e-58
Identity = 134/348 (38.51%), Postives = 206/348 (59.20%), Query Frame = 0
Query: 136 KKNFAFISSSLEKLFDKRYFCLSTRMVTITESIGVCYSSSGNNLPDAREVVQLYKSNGIE 195
KK F F S + + ++ VT SIG+ Y G+NLP V+ LYKS GI
Sbjct: 6 KKLFLFFLSCIVLYVNSN----NSGFVTAANSIGLNYGLLGDNLPSPSNVINLYKSIGIS 65
Query: 196 KMRIYNPDAAMLNALRG-SNIEVIVGIPNTDLEHIVNLSSAM-NWVQRNIQAYMADVKFR 255
++RI++P+ +LNALRG +IEV VG+ + DL + A+ W NI++Y+ADV
Sbjct: 66 RIRIFDPNTEVLNALRGHRDIEVTVGVKDQDLAALAASEEAVKGWFAANIESYLADVNIT 125
Query: 256 YIAIGNEVQPSDAVARYVLPAMNSIYSAILAANLQDQIKVSTVISISFLSNSSFPPSFSS 315
+I +GNEV P + VLP M S+ + + + NL I +STV+++S L S+PPS
Sbjct: 126 FITVGNEVIPG-PIGPQVLPVMQSLTNLVKSRNL--PISISTVVAMSNL-EQSYPPSAGM 185
Query: 316 FSSEAGGFMEPIVNFLAKNGSPLLANVYPYFTYIDITRTISLDYALFMQSPALTIRDGNF 375
F+S+A + P++ L++ +P+L N+YPYF Y I LDYA F + ++ ++DG+
Sbjct: 186 FTSQAREQLVPVLKLLSQTSTPILVNIYPYFAYASDPANIRLDYASF-NTKSIVVQDGSL 245
Query: 376 EYNSLFEAMVDALYVALEKSGGAEVSIVISESGWPS-GGGTAATVENAGTYYRNLISFVR 435
Y+++F+A+ DA A+EK G + +V+SE+GWPS G G T A TY RN + +
Sbjct: 246 GYSNMFDAIFDAFVWAMEKEGVKNLPMVVSETGWPSAGNGNFTTPAIASTYNRNFVKHIA 305
Query: 436 G--GTPRRPRRAIETYLFAMFDENNKAPEMEKHFGLFT-ADKKSKYQL 478
GTP+RP +++ +LFA F+EN K E++FGL+ +D K Y+L
Sbjct: 306 SGKGTPKRPNKSMNGFLFATFNENQKPAGTEQNFGLYNPSDMKPIYKL 344
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_004139045.1 | 3.6e-162 | 67.49 | glucan endo-1,3-beta-glucosidase [Cucumis sativus] >KAE8653516.1 hypothetical pr... | [more] |
XP_022961205.1 | 5.7e-160 | 72.33 | glucan endo-1,3-beta-glucosidase-like isoform X1 [Cucurbita moschata] | [more] |
XP_022154372.1 | 5.7e-160 | 66.04 | glucan endo-1,3-beta-glucosidase-like [Momordica charantia] | [more] |
XP_038880622.1 | 9.7e-160 | 71.46 | glucan endo-1,3-beta-glucosidase-like [Benincasa hispida] | [more] |
KAG6590190.1 | 1.7e-159 | 74.09 | Lichenase, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
Match Name | E-value | Identity | Description | |
A7PQW3 | 1.8e-100 | 58.84 | Glucan endo-1,3-beta-glucosidase OS=Vitis vinifera OX=29760 GN=VIT_06s0061g00120... | [more] |
P07979 | 5.4e-97 | 54.43 | Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3 | [more] |
P52407 | 2.3e-95 | 54.63 | Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform OS=Hevea brasiliensis O... | [more] |
Q8VZJ2 | 1.3e-90 | 51.56 | Probable glucan endo-1,3-beta-glucosidase At4g16260 OS=Arabidopsis thaliana OX=3... | [more] |
P52408 | 1.3e-90 | 53.50 | Glucan endo-1,3-beta-glucosidase, basic isoform OS=Prunus persica OX=3760 GN=GNS... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1DLI3 | 2.7e-160 | 66.04 | (1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111021653 ... | [more] |
A0A6J1HBJ9 | 2.7e-160 | 72.33 | (1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461786 P... | [more] |
A0A6J1H9Q0 | 2.8e-149 | 85.63 | (1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461786 P... | [more] |
A0A6J1DNI9 | 8.1e-128 | 55.99 | (1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111021658 ... | [more] |
A0A6J1DNH1 | 1.7e-122 | 57.75 | (1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111021643 ... | [more] |