Spg021874 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg021874
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionE3 ubiquitin-protein ligase SIS3-like
Locationscaffold2: 5561838 .. 5566096 (+)
RNA-Seq ExpressionSpg021874
SyntenySpg021874
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATCTTACGAGAACTAGCTCTTGTTCTCGTGATTGTTTCCGATTCATTGCCATCAAATTCGATCAGGCTTCTGCACCGTCCGTATGGCGGTTCGTGATTCTCCATCCAGCTTGTTCCAGTGAAGAATTCTCCCATCCATGGCTGCTCGAGGCGTCGATTTCAAGTGGTGAGTCTTCAACGACGCGCTCTCTAATTCAATGAATTTCTGTTTTAACTTGCTGTCTGGTTTGGTTGAAGAGAAATACTATGCAGTCAACTTGATAGGTTTTGGACTCCGTATTTGATTCTTGCAGGTATGACGGGTTTTTCTTGTCGATGCTCGCGACTAGTGTGTATCCTTGATTGTTTTGAGGAATTTTAGAATTTCTTGTACGGGATTCTTTTTAGTCTCTTGGAATGGCTGAGTTATGACTATTAACTGATGCGTTTACTTGCGTGGACTGCCTTTCCACTCTTTGAAGCTCGAGTGAATTTGCTCTCTAAGTTTTGCTTTGGCATTAAGAGTTGGAAATCAAACCTAATCATATCATATGCTTTGAAATCTCGAGCTATGTTTTTGATTTGTGTATCTTCTTTAACCATTTTGCTCGATAGAGTAATAGTTTCGATCAATTGGAAGAAGTATCATACTTGTGTACATCCTTTGCATATATGGATTGTGGTAAGTTCCACTTAACTTTGAACAAGTATTAGATATTATTACCGTTTTGTTGTTTTCTCGCGATTTTTTTTTGGTCCAATGATAGTCAGTTTTTGTTTTTTGTACAGGTTGATTATACTACCGTCTTTGTTTTTCGCCTGCTGATGTTTGTAGATAATGGACTTGCTGCTGGAATGGGTTTGTAAGGCTTTCAACTTTTAGTTTTACGTCAATCTAGGGTAGCAAAATCTTACGGTTTTTATTTATTTGCTTGTTTATTAAATCCCCATTAGCAAAAAGCTTTAAGGAGGTATCCTGTTGTCAGAAAATAAATTAGAGAAAAATGTTCCTTCAATACCTTTATTATGGGAAGTTTTAATTCTTGGGGGAAATATCAAAACAAACATGGATTCTGACCTGAAGAATTGTCAACCTTTGACTCATCATCAGAAGCATGGTGACAAAGGTGTGAAAAAGAAAAGAAAGAACATCTGTTGCTGATTTAAAGAATTCTGTGCTGTTTTCTAATTTTATTTTATTCTTTTGGGACTGTGACCACAAAGCTTCAAGCCATGAATGTCCACTTTCTAGTTTTGGTAGAATGATTATAAACATTATTCCCATTTAGGAATTGGAATGCTGCACAAGTTTTGCCATAAAAGTCAGTTACTTTTGACCAAGTAGGTCTTGACGTAAGACCTGTTAAATTTGTTTACCCCTGAATTAATTATTTAGTAAATGCATGCACACCTGTCTTTTTTACTCCCCATCTTCTGATGGATATTGGTTCACTGCTGGCTAAATATGAGAAAGTTATGATCCTATAATTTGAATATTTTGGTTCTGATCTATCATCCCAATTGATGTTCTTAATCTTAGATTCTTAATTGCCAATACATTTTCCTCTCTTTTTCAGTATTTCTTGTATAGTTGATGCCATTCGGGATATTATCTTCTAAAGTTGTGTATCATTAGGGACTTTGGGTGGCAGCAGAGGTATGCTCGATTTTGTGGAAGAATTGTGGTCCTTTCAGTTCTTGCGCTTCTGCTATATCCATTTCTTTGGGCTTGGACTGCCATTGGTACTTTATGGTTTAGAGAAGCCCATAAATGTGTATGTTATTTTCTATTTCAACTCCCATCGCTACCACCAGAATCTTCATATTTCTACAAATGTTTATTGAGGTATTGGTTTAAATGTGTCAGCTTCCTGAGCAAGGACAGAAATGGGGATTCCTAATTTGGTTACTTTTCAGCTACTGTGCACTCGTATGCATTGCTTGCATGTCATTGGGAAAGGTCAGTTCTATTATGTTTACTATGTAGGTTATTATTGGAGTTTATTATGCTTATGTCTAGTACTCTCCAATAATTTATAGTTTAGCTAACAGTTATTTTTATGGTGCAATTTTTATATTATTTTTTTTTCATGAGTTCTTGTTGAAAATTCTCGAATGTTTTTCTGTATCTATACATGAAATTCACGTGGTTATCTTTGAAATCTCCATGTAGTGGTTAACTCGTAGACAAGCACTTTTGTTTCGGGCGCAACAAGGGATCCCTGTATCAGAATATGGGGTAAGCAATTAAGCTTCCTGCATCTTAAAATTTTTCAACTGAAAAGACGTGCATCAATTTTCACATAGTCAATTTCTCTACCCCTTATGTTCACAATGGAATCTATAAAGTATAAATCACCTTATTTTATATTTATATCTTCTTTAAATTCAATTTTTTGCCCTGGAGATTCTTAAAAATGTAAACACATATGATGGATTGATCATGAATGAAGTTAAAATGGGGGGAAAACACTTTGAACTGGATAATAGTTCAATCAAAGTCACTAATATTTCTATCCTTACTGTTTTCTTCAGTTCTTTTTAGTATATGATTCTATTCTTAAAAATGAAGATTTGAAAATTTCATTTTCTTGCATCAGCATAACATGTTTAAGGACAATACACTTCTTGGGCAACCAAATAAATATTTAAGTGTATTTTATTCCTTTTTTTACCTCTATAATTTTGCTCATGTGACAATACTGATAGTGACAACAATTTTTTATTACAGGTCTTGGTTGACATGATCCGAGTACCTGATTGGGCTTTTGAAGCTGCAGGACAAGAAACAAGGGTAATGAGCCAAGATGCTGCTACATACCATCCCGGACTTTACTTGACTCCAGCTCAGGTAAGTCTATTAGCACTTGAATTGACATTTTGTCATCAATATTCTTGAGCTTTCCCCAAACACCTGTTGTCTGGTAGCATAAGGGTAGTTTTGTGCTGTTTTCACTATTTTCCTTCTTAGTGATTGTACAAATTGTTCGTAAAAGTTTGAGGTAATTTGATTAACGTAATAGTATCGTGTATGCTGAAGTTCTTTGCAAAGAGGGCTTGGTGTCATAGGTTGTCGGCCTTTAAACTTTTCACGAGAGATCTTGTCTAAATCTTAAAGGAATCTGTCTGCCACGTAAAGCCATAGGGTCCAAATTTATGATTGAATCTTTTGTTTTAAATTCTGATGGTCAAGCTATTCGTGGATACTTGGACAGAGGGAAGCCGTGGAAGCACTTATTGAGGAACTTCCCAAATTCAGGTTAAAGGCTGTTCCAACAGATTGCAGTGAATGTCCTATCTGCTTAGAAGAGTTCCATGTAGGGAATGAGGTAATTGGTTTTTTGTCTTCATTTAATTTACAGAAGTTCGGAAAACTTTTGGGTGTAAAAAAATAAAAAATAAAAAAACTTCCGTTCATTTATGATTTGTATTTTACTGCTTTTCATGCTCTTTCTGGGACCCTCAAGCTACAGAAGTTGGAGGAATCCTAGATTCATGCTTATAAATACATAAAAGCATTAACGTTTTGTTGGTGAATTATTGAAAATAAAGTTGCGGGCATTGTTTGTACATTGGTGCAGGTTCGTGGTCTCCCTTGTGCTCATAATTTCCATGTAGGATGTATCGACGAGTGGCTTCGACTGAATGTGAAATGCCCTAGATGTCGTTGTTCAGTCTTCCCAAACCTTGACCTCAGTGCTCTGTCCAATCTTCGTTCTGATACTGATAGGCCATCCGCAAGTGTTGTCACGGCCAATCGTTATGTGAGATCTCAACCTTCCAGCCAAAGTTATCTGGTGAGGTTGCAGGGTCTGCTTCGACCTGTTCGCACGGAAAATATTGGTGCACACAGTGATTTAGAGTCTGCTAGTGCTTTGGAAGCTGCTGAGAATGGGTCTGCCTATGACCAAACTATACCAGAACGGTCTCCTGTAAGACCCATGTCTTTGAGTCAGTCCACATCACAAGTCTAGGAAGTTGGGGGTGTTATTCTCCCTTGCTATTTTGACTGCTTCTAACTGCTCCTGTAAAAGCTAAACGATAAGTGTCCAGTGTCCACATCTTGCCATTTGCAAATCCCGGCAACTGTGTAATAGTAAAGCTCGAACGTTTGTTTTTTTGTATCCAAGGAATCTACTTACTCACGTTCTAGCAAAACTCAATCTTTTCATTGTTCACTTTTAGCTGTATCATGTTCATCAAAATTAATTTTAGATCTTAGTACTGGACTATTCAGCTGCCTGTAAAGGGATGAAATTGGTCATGTGCTGTTTGCAG

mRNA sequence

ATGATCTTACGAGAACTAGCTCTTGTTCTCGTGATTGTTTCCGATTCATTGCCATCAAATTCGATCAGGCTTCTGCACCGTCCGTATGGCGCTCGAGTGAATTTGCTCTCTAAGTTTTGCTTTGGCATTAAGAGTTGGAAATCAAACCTAATCATATCATATGCTTTGAAATCTCGAGCTATGTTTTTGATTTGTGTATCTTCTTTAACCATTTTGCTCGATAGAGTAATAGTTTCGATCAATTGGAAGAAGTATCATACTTGTGTACATCCTTTGCATATATGGATTGTGGTTGATTATACTACCGTCTTTGTTTTTCGCCTGCTGATGTTTGTAGATAATGGACTTGCTGCTGGAATGGGTTTGGACTTTGGGTGGCAGCAGAGGTATGCTCGATTTTGTGGAAGAATTGTGGTCCTTTCAGTTCTTGCGCTTCTGCTATATCCATTTCTTTGGGCTTGGACTGCCATTGGTACTTTATGGTTTAGAGAAGCCCATAAATGTCTTCCTGAGCAAGGACAGAAATGGGGATTCCTAATTTGGTTACTTTTCAGCTACTGTGCACTCGTATGCATTGCTTGCATGTCATTGGGAAAGGTCTTGGTTGACATGATCCGAGTACCTGATTGGGCTTTTGAAGCTGCAGGACAAGAAACAAGGGTAATGAGCCAAGATGCTGCTACATACCATCCCGGACTTTACTTGACTCCAGCTCAGAGGGAAGCCGTGGAAGCACTTATTGAGGAACTTCCCAAATTCAGGTTAAAGGCTGTTCCAACAGATTGCAGTGAATGTCCTATCTGCTTAGAAGAGTTCCATGTAGGGAATGAGGTTCGTGGTCTCCCTTGTGCTCATAATTTCCATGTAGGATGTATCGACGAGTGGCTTCGACTGAATGTGAAATGCCCTAGATGTCGTTGTTCAGTCTTCCCAAACCTTGACCTCAGTGCTCTGTCCAATCTTCGTTCTGATACTGATAGGCCATCCGCAAGTGTTGTCACGGCCAATCGTTATGTGAGATCTCAACCTTCCAGCCAAAGTTATCTGGTGAGGTTGCAGGGTCTGCTTCGACCTGTTCGCACGGAAAATATTGGTGCACACAGTGATTTAGAGTCTGCTAGTGCTTTGGAAGCTGCTGAGAATGGGTCTGCCTATGACCAAACTATACCAGAACGGTCTCCTGTAAGACCCATGTCTTTGAGTCAGTCCACATCACAAGTCTAG

Coding sequence (CDS)

ATGATCTTACGAGAACTAGCTCTTGTTCTCGTGATTGTTTCCGATTCATTGCCATCAAATTCGATCAGGCTTCTGCACCGTCCGTATGGCGCTCGAGTGAATTTGCTCTCTAAGTTTTGCTTTGGCATTAAGAGTTGGAAATCAAACCTAATCATATCATATGCTTTGAAATCTCGAGCTATGTTTTTGATTTGTGTATCTTCTTTAACCATTTTGCTCGATAGAGTAATAGTTTCGATCAATTGGAAGAAGTATCATACTTGTGTACATCCTTTGCATATATGGATTGTGGTTGATTATACTACCGTCTTTGTTTTTCGCCTGCTGATGTTTGTAGATAATGGACTTGCTGCTGGAATGGGTTTGGACTTTGGGTGGCAGCAGAGGTATGCTCGATTTTGTGGAAGAATTGTGGTCCTTTCAGTTCTTGCGCTTCTGCTATATCCATTTCTTTGGGCTTGGACTGCCATTGGTACTTTATGGTTTAGAGAAGCCCATAAATGTCTTCCTGAGCAAGGACAGAAATGGGGATTCCTAATTTGGTTACTTTTCAGCTACTGTGCACTCGTATGCATTGCTTGCATGTCATTGGGAAAGGTCTTGGTTGACATGATCCGAGTACCTGATTGGGCTTTTGAAGCTGCAGGACAAGAAACAAGGGTAATGAGCCAAGATGCTGCTACATACCATCCCGGACTTTACTTGACTCCAGCTCAGAGGGAAGCCGTGGAAGCACTTATTGAGGAACTTCCCAAATTCAGGTTAAAGGCTGTTCCAACAGATTGCAGTGAATGTCCTATCTGCTTAGAAGAGTTCCATGTAGGGAATGAGGTTCGTGGTCTCCCTTGTGCTCATAATTTCCATGTAGGATGTATCGACGAGTGGCTTCGACTGAATGTGAAATGCCCTAGATGTCGTTGTTCAGTCTTCCCAAACCTTGACCTCAGTGCTCTGTCCAATCTTCGTTCTGATACTGATAGGCCATCCGCAAGTGTTGTCACGGCCAATCGTTATGTGAGATCTCAACCTTCCAGCCAAAGTTATCTGGTGAGGTTGCAGGGTCTGCTTCGACCTGTTCGCACGGAAAATATTGGTGCACACAGTGATTTAGAGTCTGCTAGTGCTTTGGAAGCTGCTGAGAATGGGTCTGCCTATGACCAAACTATACCAGAACGGTCTCCTGTAAGACCCATGTCTTTGAGTCAGTCCACATCACAAGTCTAG

Protein sequence

MILRELALVLVIVSDSLPSNSIRLLHRPYGARVNLLSKFCFGIKSWKSNLIISYALKSRAMFLICVSSLTILLDRVIVSINWKKYHTCVHPLHIWIVVDYTTVFVFRLLMFVDNGLAAGMGLDFGWQQRYARFCGRIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQGQKWGFLIWLLFSYCALVCIACMSLGKVLVDMIRVPDWAFEAAGQETRVMSQDAATYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSASVVTANRYVRSQPSSQSYLVRLQGLLRPVRTENIGAHSDLESASALEAAENGSAYDQTIPERSPVRPMSLSQSTSQV
Homology
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BLAST of Spg021874 vs. NCBI nr
Match: XP_008453098.1 (PREDICTED: E3 ubiquitin-protein ligase SIS3-like [Cucumis melo])

HSP 1 Score: 647.1 bits (1668), Expect = 1.0e-181
Identity = 320/354 (90.40%), Postives = 323/354 (91.24%), Query Frame = 0 

Query: 76  VIVSINWKKYHTCVHPLHIWIVVDYTTVFVFRLLMFVDNGLAAGMGLDFGWQQRYARFCG 135
           VIVSINWKKYHTCVHPLHIWIVVDYTTVFVFRLLMFVDNGLAAGMGLDFGWQQRYARFCG
Sbjct: 24  VIVSINWKKYHTCVHPLHIWIVVDYTTVFVFRLLMFVDNGLAAGMGLDFGWQQRYARFCG 83

Query: 136 RIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQGQKWGFLIWLLFSYCALVCIACM 195
           RIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQGQKWGFLIWLLFSYCALVCIACM
Sbjct: 84  RIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQGQKWGFLIWLLFSYCALVCIACM 143

Query: 196 SLGK----------------------VLVDMIRVPDWAFEAAGQETRVMSQDAATYHPGL 255
           SLGK                      VLVDMIRVPDWAFEAAGQETRVM QDAA YHPGL
Sbjct: 144 SLGKWLTRRQALLFRAQQGFPVSEYGVLVDMIRVPDWAFEAAGQETRVMGQDAAAYHPGL 203

Query: 256 YLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCID 315
           YLTPAQREAVEALIEELP+FRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCID
Sbjct: 204 YLTPAQREAVEALIEELPEFRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCID 263

Query: 316 EWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSASVVTANRYVRSQPSSQSYLVRLQ 375
           EWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSASVVTANRYV SQPSSQSYLVRLQ
Sbjct: 264 EWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSASVVTANRYVGSQPSSQSYLVRLQ 323

Query: 376 GLLRPVRTENIGAHSDLESASALEAAENGSAYDQTIPERSPVRPMSLSQSTSQV 408
           GLLRPVRTEN  AH DL  ASALEAAENGSA+DQTIPERSPVRP+SL QSTSQV
Sbjct: 324 GLLRPVRTENNDAHGDLGPASALEAAENGSAHDQTIPERSPVRPLSLIQSTSQV 377

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BLAST of Spg021874 vs. NCBI nr
Match: XP_004138113.1 (E3 ubiquitin-protein ligase SIS3 [Cucumis sativus] >KGN63587.1 hypothetical protein Csa_013206 [Cucumis sativus])

HSP 1 Score: 640.2 bits (1650), Expect = 1.2e-179
Identity = 314/354 (88.70%), Postives = 322/354 (90.96%), Query Frame = 0 

Query: 76  VIVSINWKKYHTCVHPLHIWIVVDYTTVFVFRLLMFVDNGLAAGMGLDFGWQQRYARFCG 135
           VIVSINWK+YHTCVHPLHIWIVVDYTTVFVFRLLMFVDNGLAAGMGLDFGWQQRYARFCG
Sbjct: 24  VIVSINWKRYHTCVHPLHIWIVVDYTTVFVFRLLMFVDNGLAAGMGLDFGWQQRYARFCG 83

Query: 136 RIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQGQKWGFLIWLLFSYCALVCIACM 195
           RIVVLSVLA+LLYPFLWAWTAIGT+WFREAHKCLPEQGQKWGFLIWLLFSYCALVCIACM
Sbjct: 84  RIVVLSVLAVLLYPFLWAWTAIGTIWFREAHKCLPEQGQKWGFLIWLLFSYCALVCIACM 143

Query: 196 SLGK----------------------VLVDMIRVPDWAFEAAGQETRVMSQDAATYHPGL 255
           SLGK                      VLVDM+RVPDWAFEAAGQETRVM QDAA YHPGL
Sbjct: 144 SLGKWLTRRQALLFRAQQGIPVSEYGVLVDMVRVPDWAFEAAGQETRVMGQDAAAYHPGL 203

Query: 256 YLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCID 315
           YLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCID
Sbjct: 204 YLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCID 263

Query: 316 EWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSASVVTANRYVRSQPSSQSYLVRLQ 375
           EWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSASV+TANRYV SQPSSQSYLVRLQ
Sbjct: 264 EWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSASVLTANRYVGSQPSSQSYLVRLQ 323

Query: 376 GLLRPVRTENIGAHSDLESASALEAAENGSAYDQTIPERSPVRPMSLSQSTSQV 408
           GLLRPVRTE   AH DL  ASALEAAENGSA++QTIPERSPVRPMSL QSTSQ+
Sbjct: 324 GLLRPVRTEENDAHGDLGPASALEAAENGSAHEQTIPERSPVRPMSLIQSTSQL 377

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BLAST of Spg021874 vs. NCBI nr
Match: XP_038880106.1 (E3 ubiquitin-protein ligase SIS3-like [Benincasa hispida])

HSP 1 Score: 638.3 bits (1645), Expect = 4.6e-179
Identity = 314/354 (88.70%), Postives = 321/354 (90.68%), Query Frame = 0 

Query: 76  VIVSINWKKYHTCVHPLHIWIVVDYTTVFVFRLLMFVDNGLAAGMGLDFGWQQRYARFCG 135
           V+VSINWK+YHTCVHPLHIWIVVDYT VFVFRLLMFVDNGLAAGMGLDFGWQQRYARFCG
Sbjct: 24  VMVSINWKRYHTCVHPLHIWIVVDYTAVFVFRLLMFVDNGLAAGMGLDFGWQQRYARFCG 83

Query: 136 RIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQGQKWGFLIWLLFSYCALVCIACM 195
           RIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQGQKWGFLIWLLFSYCALVCIACM
Sbjct: 84  RIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQGQKWGFLIWLLFSYCALVCIACM 143

Query: 196 SLGK----------------------VLVDMIRVPDWAFEAAGQETRVMSQDAATYHPGL 255
           SLGK                      VLVDMIRVPDWAFEAAGQETRVM QDAA YHPGL
Sbjct: 144 SLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDWAFEAAGQETRVMGQDAAAYHPGL 203

Query: 256 YLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCID 315
           YLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCID
Sbjct: 204 YLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCID 263

Query: 316 EWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSASVVTANRYVRSQPSSQSYLVRLQ 375
           EWLRLNVKCPRCRCSVFPNLDLSALSNLRSD DRPSASVVTAN Y+RSQPSSQSYLVRLQ
Sbjct: 264 EWLRLNVKCPRCRCSVFPNLDLSALSNLRSDVDRPSASVVTANHYMRSQPSSQSYLVRLQ 323

Query: 376 GLLRPVRTENIGAHSDLESASALEAAENGSAYDQTIPERSPVRPMSLSQSTSQV 408
           GLLRPVRTE+  A SDLESA+ALEAAEN S +DQTIP RSPVRPMSLSQSTSQ+
Sbjct: 324 GLLRPVRTESTDAQSDLESANALEAAENASIHDQTIPGRSPVRPMSLSQSTSQL 377

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BLAST of Spg021874 vs. NCBI nr
Match: XP_023516682.1 (E3 ubiquitin-protein ligase SIS3-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 634.4 bits (1635), Expect = 6.7e-178
Identity = 313/356 (87.92%), Postives = 322/356 (90.45%), Query Frame = 0 

Query: 76  VIVSINWKKYHTCVHPLHIWIVVDYTTVFVFRLLMFVDNGLAAGMGLDFGWQQRYARFCG 135
           V+VSINWK+YHTCVHPLHIWI+VDYTTVFVFRLLMFVDNGLAAGMGLDFGWQQRYARFCG
Sbjct: 24  VVVSINWKRYHTCVHPLHIWILVDYTTVFVFRLLMFVDNGLAAGMGLDFGWQQRYARFCG 83

Query: 136 RIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQGQKWGFLIWLLFSYCALVCIACM 195
           RIVVLS+LALLLYPFLWAWTAIGTLWFREAHKCL EQGQKWGFLIWLLFSYCALVCIAC+
Sbjct: 84  RIVVLSILALLLYPFLWAWTAIGTLWFREAHKCLFEQGQKWGFLIWLLFSYCALVCIACV 143

Query: 196 SLGK----------------------VLVDMIRVPDWAFEAAGQETRVMSQDAATYHPGL 255
           SLGK                      VLVDMIRVPDWAFEAAGQETRVM QDAA YHPGL
Sbjct: 144 SLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDWAFEAAGQETRVMGQDAAAYHPGL 203

Query: 256 YLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCID 315
           YLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCID
Sbjct: 204 YLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCID 263

Query: 316 EWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSASVVTANRYVRSQPSSQSYLVRLQ 375
           EWLRLNVKCPRCRCSVFPNLDLSALSNL SDT+RPSASVVTANRY+R QPSSQSYLVRLQ
Sbjct: 264 EWLRLNVKCPRCRCSVFPNLDLSALSNLHSDTERPSASVVTANRYMRPQPSSQSYLVRLQ 323

Query: 376 GLLRPVRTENIGAHSDLESASALEA---AENGSAYDQTIPERSPVRPMSLSQSTSQ 407
           GLLRPVRTEN  AH DLESA ALEA   AENGS++DQTIPERSPVRPMSLSQSTSQ
Sbjct: 324 GLLRPVRTENTVAHGDLESAGALEAAENAENGSSHDQTIPERSPVRPMSLSQSTSQ 379

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BLAST of Spg021874 vs. NCBI nr
Match: KAG7023134.1 (E3 ubiquitin-protein ligase SIS3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 632.5 bits (1630), Expect = 2.5e-177
Identity = 315/379 (83.11%), Postives = 330/379 (87.07%), Query Frame = 0 

Query: 51  IISYALKSRAM---FLICVSSLTILLDRVIVSINWKKYHTCVHPLHIWIVVDYTTVFVFR 110
           I+ + +KS      F ICV SLTILLDRV+VSINWK+YH CVHPLHIWI+VDYTTVFVFR
Sbjct: 54  ILCFKIKSNVFLLTFFICVFSLTILLDRVVVSINWKRYHPCVHPLHIWILVDYTTVFVFR 113

Query: 111 LLMFVDNGLAAGMGLDFGWQQRYARFCGRIVVLSVLALLLYPFLWAWTAIGTLWFREAHK 170
           LLMFVDNGLAAGMGLDFGWQQRYARFCGRIVVLS+LALLLYPFLWAWTAIGTLWFREAHK
Sbjct: 114 LLMFVDNGLAAGMGLDFGWQQRYARFCGRIVVLSILALLLYPFLWAWTAIGTLWFREAHK 173

Query: 171 CLPEQGQKWGFLIWLLFSYCALVCIACMSLGK----------------------VLVDMI 230
           CL EQGQKWGFLIWLLFSYCALVCIAC+SLGK                      VLVDMI
Sbjct: 174 CLFEQGQKWGFLIWLLFSYCALVCIACVSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMI 233

Query: 231 RVPDWAFEAAGQETRVMSQDAATYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSEC 290
           RVPDWAFEAAGQETRVM QDAA YHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSEC
Sbjct: 234 RVPDWAFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSEC 293

Query: 291 PICLEEFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLRSDT 350
           PICLEEFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNL+SDT
Sbjct: 294 PICLEEFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLQSDT 353

Query: 351 DRPSASVVTANRYVRSQPSSQSYLVRLQGLLRPVRTENIGAHSDLESASALEA---AENG 402
           +RPSASVVTANRY+  QPSSQSYLVRLQGLLRPVRTEN  AH DLESASALE    AENG
Sbjct: 354 ERPSASVVTANRYMSPQPSSQSYLVRLQGLLRPVRTENTVAHGDLESASALEVAEDAENG 413

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BLAST of Spg021874 vs. ExPASy Swiss-Prot
Match: Q8GYT9 (E3 ubiquitin-protein ligase SIS3 OS=Arabidopsis thaliana OX=3702 GN=SIS3 PE=2 SV=2)

HSP 1 Score: 459.9 bits (1182), Expect = 3.0e-128
Identity = 226/346 (65.32%), Postives = 262/346 (75.72%), Query Frame = 0 

Query: 76  VIVSINWKKYHTCVHPLHIWIVVDYTTVFVFRLLMFVDNGLAAGMGLDFGWQQRYARFCG 135
           +IV++NW +Y  C +PLHIWIVVDYTTVF+FR+ MFVDNGLA+G+GLDFG QQR A FCG
Sbjct: 24  IIVAVNWNRYRACEYPLHIWIVVDYTTVFIFRVFMFVDNGLASGLGLDFGSQQRNAMFCG 83

Query: 136 RIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQGQKWGFLIWLLFSYCALVCIACM 195
           R+VVLSVL+LLLYPFLWAWT IGT WF ++  CLPE+GQKWGFLIWL+FSYC L+CIA +
Sbjct: 84  RVVVLSVLSLLLYPFLWAWTVIGTQWFTKSKTCLPEEGQKWGFLIWLMFSYCGLLCIAFI 143

Query: 196 SLGK----------------------VLVDMIRVPDWAFEAAGQETRVMSQDAATYHPGL 255
            +GK                      +LVDMIRVPDWAFEAAGQE R +SQDAATYHPGL
Sbjct: 144 CVGKWLTRRQVHLLRAQQGIPISEFGILVDMIRVPDWAFEAAGQEMRGISQDAATYHPGL 203

Query: 256 YLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCID 315
           YLTPAQ EAVEALI+ELPKFRLKAVP DC EC ICLEEFH+G+EVRGLPCAHNFHV CID
Sbjct: 204 YLTPAQTEAVEALIQELPKFRLKAVPDDCGECLICLEEFHIGHEVRGLPCAHNFHVECID 263

Query: 316 EWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSASVVTAN---RYVRSQPSSQSYLV 375
           +WLRLNVKCPRCRCSVFP+LDLSALSNL+S      + V T     RY+RSQP S+SY +
Sbjct: 264 QWLRLNVKCPRCRCSVFPDLDLSALSNLQSSGTEQHSQVNTETSEARYIRSQPQSESYFL 323

Query: 376 RLQGLLRPVRTENIGAHSDLESASALEAAENGSAYDQTIPERSPVR 397
           R+Q L+ PV T+           +ALE AENG      + + SP R
Sbjct: 324 RVQSLIHPVHTD-----------TALETAENGGV-PPVLTDLSPSR 357

[Close]
BLAST of Spg021874 vs. ExPASy Swiss-Prot
Match: Q93Z92 (E3 ubiquitin-protein ligase At4g11680 OS=Arabidopsis thaliana OX=3702 GN=At4g11680 PE=2 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 2.3e-11
Identity = 36/107 (33.64%), Postives = 53/107 (49.53%), Query Frame = 0 

Query: 233 LYLTPAQREAVEALIEELPKFRL-------------KAVPTDC--------------SEC 292
           LY    Q  A +  I+++PKFR              + + T+C              +EC
Sbjct: 279 LYAVADQEGASKNDIDQMPKFRFTKTGNVEKLSGKARGIMTECGTDSPIERSLSPEDAEC 338

Query: 293 PICLEEFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPN 313
            ICL E+  G E+R LPC H+FH  CID+WL +N +CP C+ ++  N
Sbjct: 339 CICLCEYEDGVELRELPCNHHFHCTCIDKWLHINSRCPLCKFNILKN 385

[Close]
BLAST of Spg021874 vs. ExPASy Swiss-Prot
Match: Q8LDB8 (E3 ubiquitin-protein ligase At1g63170 OS=Arabidopsis thaliana OX=3702 GN=At1g63170 PE=2 SV=2)

HSP 1 Score: 68.6 bits (166), Expect = 1.9e-10
Identity = 24/53 (45.28%), Postives = 36/53 (67.92%), Query Frame = 0 

Query: 257 AVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSV 310
           A+P + +EC ICL  +    E+R LPC H+FH GC+D+WL +N  CP C+ ++
Sbjct: 317 ALPHEDAECCICLSAYEDETELRELPCGHHFHCGCVDKWLYINATCPLCKYNI 369

[Close]
BLAST of Spg021874 vs. ExPASy Swiss-Prot
Match: Q9SK92 (E3 ubiquitin-protein ligase ATL15 OS=Arabidopsis thaliana OX=3702 GN=ATL15 PE=1 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 2.5e-10
Identity = 31/73 (42.47%), Postives = 39/73 (53.42%), Query Frame = 0 

Query: 245 ALIEELPKFRLKAVPT-----DCSECPICLEEFHVGNEVRGLP-CAHNFHVGCIDEWLRL 304
           ++IE  P F    V T     +  ECP+CL EF     +R +P C H FH GCID WLR 
Sbjct: 93  SVIETFPTFPYSTVKTLRIGKEALECPVCLNEFEDDETLRLIPQCCHVFHPGCIDAWLRS 152

Query: 305 NVKCPRCRCSVFP 312
              CP CR ++ P
Sbjct: 153 QTTCPLCRANLVP 165

[Close]
BLAST of Spg021874 vs. ExPASy Swiss-Prot
Match: Q9SL78 (Putative RING-H2 finger protein ATL12 OS=Arabidopsis thaliana OX=3702 GN=ATL12 PE=3 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 4.3e-10
Identity = 35/77 (45.45%), Postives = 42/77 (54.55%), Query Frame = 0 

Query: 247 IEELPKFRLKAVP--TDCSECPICLEEFHVGNEVRGLP-CAHNFHVGCIDEWLRLNVKCP 306
           IE LP FR  A+       EC +CL +F     +R LP C H FH+GCID+WL  +  CP
Sbjct: 104 IESLPFFRFSALKGLKQGLECSVCLSKFEDVEILRLLPKCRHAFHIGCIDQWLEQHATCP 163

Query: 307 RCRCSVFPNLDLSALSN 321
            CR  V    DLS L N
Sbjct: 164 LCRNRVNIEDDLSVLGN 180

[Close]
BLAST of Spg021874 vs. ExPASy TrEMBL
Match: A0A1S3BVE9 (E3 ubiquitin-protein ligase SIS3-like OS=Cucumis melo OX=3656 GN=LOC103493914 PE=4 SV=1)

HSP 1 Score: 647.1 bits (1668), Expect = 4.8e-182
Identity = 320/354 (90.40%), Postives = 323/354 (91.24%), Query Frame = 0 

Query: 76  VIVSINWKKYHTCVHPLHIWIVVDYTTVFVFRLLMFVDNGLAAGMGLDFGWQQRYARFCG 135
           VIVSINWKKYHTCVHPLHIWIVVDYTTVFVFRLLMFVDNGLAAGMGLDFGWQQRYARFCG
Sbjct: 24  VIVSINWKKYHTCVHPLHIWIVVDYTTVFVFRLLMFVDNGLAAGMGLDFGWQQRYARFCG 83

Query: 136 RIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQGQKWGFLIWLLFSYCALVCIACM 195
           RIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQGQKWGFLIWLLFSYCALVCIACM
Sbjct: 84  RIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQGQKWGFLIWLLFSYCALVCIACM 143

Query: 196 SLGK----------------------VLVDMIRVPDWAFEAAGQETRVMSQDAATYHPGL 255
           SLGK                      VLVDMIRVPDWAFEAAGQETRVM QDAA YHPGL
Sbjct: 144 SLGKWLTRRQALLFRAQQGFPVSEYGVLVDMIRVPDWAFEAAGQETRVMGQDAAAYHPGL 203

Query: 256 YLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCID 315
           YLTPAQREAVEALIEELP+FRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCID
Sbjct: 204 YLTPAQREAVEALIEELPEFRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCID 263

Query: 316 EWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSASVVTANRYVRSQPSSQSYLVRLQ 375
           EWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSASVVTANRYV SQPSSQSYLVRLQ
Sbjct: 264 EWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSASVVTANRYVGSQPSSQSYLVRLQ 323

Query: 376 GLLRPVRTENIGAHSDLESASALEAAENGSAYDQTIPERSPVRPMSLSQSTSQV 408
           GLLRPVRTEN  AH DL  ASALEAAENGSA+DQTIPERSPVRP+SL QSTSQV
Sbjct: 324 GLLRPVRTENNDAHGDLGPASALEAAENGSAHDQTIPERSPVRPLSLIQSTSQV 377

[Close]
BLAST of Spg021874 vs. ExPASy TrEMBL
Match: A0A0A0LS35 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G004990 PE=4 SV=1)

HSP 1 Score: 640.2 bits (1650), Expect = 5.9e-180
Identity = 314/354 (88.70%), Postives = 322/354 (90.96%), Query Frame = 0 

Query: 76  VIVSINWKKYHTCVHPLHIWIVVDYTTVFVFRLLMFVDNGLAAGMGLDFGWQQRYARFCG 135
           VIVSINWK+YHTCVHPLHIWIVVDYTTVFVFRLLMFVDNGLAAGMGLDFGWQQRYARFCG
Sbjct: 24  VIVSINWKRYHTCVHPLHIWIVVDYTTVFVFRLLMFVDNGLAAGMGLDFGWQQRYARFCG 83

Query: 136 RIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQGQKWGFLIWLLFSYCALVCIACM 195
           RIVVLSVLA+LLYPFLWAWTAIGT+WFREAHKCLPEQGQKWGFLIWLLFSYCALVCIACM
Sbjct: 84  RIVVLSVLAVLLYPFLWAWTAIGTIWFREAHKCLPEQGQKWGFLIWLLFSYCALVCIACM 143

Query: 196 SLGK----------------------VLVDMIRVPDWAFEAAGQETRVMSQDAATYHPGL 255
           SLGK                      VLVDM+RVPDWAFEAAGQETRVM QDAA YHPGL
Sbjct: 144 SLGKWLTRRQALLFRAQQGIPVSEYGVLVDMVRVPDWAFEAAGQETRVMGQDAAAYHPGL 203

Query: 256 YLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCID 315
           YLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCID
Sbjct: 204 YLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCID 263

Query: 316 EWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSASVVTANRYVRSQPSSQSYLVRLQ 375
           EWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSASV+TANRYV SQPSSQSYLVRLQ
Sbjct: 264 EWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSASVLTANRYVGSQPSSQSYLVRLQ 323

Query: 376 GLLRPVRTENIGAHSDLESASALEAAENGSAYDQTIPERSPVRPMSLSQSTSQV 408
           GLLRPVRTE   AH DL  ASALEAAENGSA++QTIPERSPVRPMSL QSTSQ+
Sbjct: 324 GLLRPVRTEENDAHGDLGPASALEAAENGSAHEQTIPERSPVRPMSLIQSTSQL 377

[Close]
BLAST of Spg021874 vs. ExPASy TrEMBL
Match: A0A6J1E7C4 (E3 ubiquitin-protein ligase SIS3-like OS=Cucurbita moschata OX=3662 GN=LOC111430080 PE=4 SV=1)

HSP 1 Score: 632.1 bits (1629), Expect = 1.6e-177
Identity = 312/356 (87.64%), Postives = 321/356 (90.17%), Query Frame = 0 

Query: 76  VIVSINWKKYHTCVHPLHIWIVVDYTTVFVFRLLMFVDNGLAAGMGLDFGWQQRYARFCG 135
           V+VSINWK+YHTCVHPLHIWI+VDYTTVFVFRLLMFVDNGLAAGMGLDFGWQQRYARFCG
Sbjct: 24  VVVSINWKRYHTCVHPLHIWILVDYTTVFVFRLLMFVDNGLAAGMGLDFGWQQRYARFCG 83

Query: 136 RIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQGQKWGFLIWLLFSYCALVCIACM 195
           RIVVLS+LALLLYPFLWAWTAIGTLWFREAHKCL EQGQKWGFLIWLLFSYCALVCIAC+
Sbjct: 84  RIVVLSILALLLYPFLWAWTAIGTLWFREAHKCLFEQGQKWGFLIWLLFSYCALVCIACV 143

Query: 196 SLGK----------------------VLVDMIRVPDWAFEAAGQETRVMSQDAATYHPGL 255
           SLGK                      VLVDMIRVPDWAFEAAGQETRVM QDAA YHPGL
Sbjct: 144 SLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDWAFEAAGQETRVMGQDAAAYHPGL 203

Query: 256 YLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCID 315
           YLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCID
Sbjct: 204 YLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCID 263

Query: 316 EWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSASVVTANRYVRSQPSSQSYLVRLQ 375
           EWLRLNVKCPRCRCSVFPNLDLSALSNL SDT+RPSASVVTANRY+R QPSSQSYLVRLQ
Sbjct: 264 EWLRLNVKCPRCRCSVFPNLDLSALSNLHSDTERPSASVVTANRYMRPQPSSQSYLVRLQ 323

Query: 376 GLLRPVRTENIGAHSDLESASALEA---AENGSAYDQTIPERSPVRPMSLSQSTSQ 407
           GLLRPVRTEN  AH DLESASALE    AENGS++DQTIPERSPVRPM LSQSTSQ
Sbjct: 324 GLLRPVRTENTVAHCDLESASALEVAENAENGSSHDQTIPERSPVRPMPLSQSTSQ 379

[Close]
BLAST of Spg021874 vs. ExPASy TrEMBL
Match: A0A6J1JJE6 (E3 ubiquitin-protein ligase SIS3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485643 PE=4 SV=1)

HSP 1 Score: 625.9 bits (1613), Expect = 1.2e-175
Identity = 308/356 (86.52%), Postives = 318/356 (89.33%), Query Frame = 0 

Query: 76  VIVSINWKKYHTCVHPLHIWIVVDYTTVFVFRLLMFVDNGLAAGMGLDFGWQQRYARFCG 135
           V+VSINWK+YHTCVHPLHIWI+VDYTTVFVFRLLMFVDNGLAAGMGLDFGWQQRYARFCG
Sbjct: 24  VVVSINWKRYHTCVHPLHIWILVDYTTVFVFRLLMFVDNGLAAGMGLDFGWQQRYARFCG 83

Query: 136 RIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQGQKWGFLIWLLFSYCALVCIACM 195
           RIVVLS+LALLLYPFLWAWTAIGTLWFREAHKCL EQGQKWGFLIWLLFSYCALVCIACM
Sbjct: 84  RIVVLSILALLLYPFLWAWTAIGTLWFREAHKCLFEQGQKWGFLIWLLFSYCALVCIACM 143

Query: 196 SLGK----------------------VLVDMIRVPDWAFEAAGQETRVMSQDAATYHPGL 255
           SLGK                      VLVDMIRVPDWAFEAAGQETRVM QDAA YHPGL
Sbjct: 144 SLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDWAFEAAGQETRVMGQDAAAYHPGL 203

Query: 256 YLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCID 315
           YLTPAQREAVEALIEELPKFRLKAVPTDC ECPICLEEFHVGNEVRGLPCAHNFHVGCID
Sbjct: 204 YLTPAQREAVEALIEELPKFRLKAVPTDCCECPICLEEFHVGNEVRGLPCAHNFHVGCID 263

Query: 316 EWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSASVVTANRYVRSQPSSQSYLVRLQ 375
           EWLRLNVKCPRCRCSVFPNLDLSALSNL SDT+RPSASVVTANRY+R QPSSQSYLVRLQ
Sbjct: 264 EWLRLNVKCPRCRCSVFPNLDLSALSNLHSDTERPSASVVTANRYMRPQPSSQSYLVRLQ 323

Query: 376 GLLRPVRTENIGAHSDLESASALEA---AENGSAYDQTIPERSPVRPMSLSQSTSQ 407
           GLLRPV TEN  AH DLESASALE    AENGS++DQT+PERSPVRP+S SQST Q
Sbjct: 324 GLLRPVHTENTVAHGDLESASALEVAENAENGSSHDQTVPERSPVRPVSSSQSTPQ 379

[Close]
BLAST of Spg021874 vs. ExPASy TrEMBL
Match: A0A6J1F591 (E3 ubiquitin-protein ligase SIS3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111440966 PE=4 SV=1)

HSP 1 Score: 619.4 bits (1596), Expect = 1.1e-173
Identity = 305/356 (85.67%), Postives = 315/356 (88.48%), Query Frame = 0 

Query: 76  VIVSINWKKYHTCVHPLHIWIVVDYTTVFVFRLLMFVDNGLAAGMGLDFGWQQRYARFCG 135
           VIVSI+WK+YHTCVHPLHIWIVVDYT VF+FRLLMFVDNGLAAGMGLDFGWQQRYARFCG
Sbjct: 24  VIVSISWKRYHTCVHPLHIWIVVDYTAVFLFRLLMFVDNGLAAGMGLDFGWQQRYARFCG 83

Query: 136 RIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQGQKWGFLIWLLFSYCALVCIACM 195
           RIVVLSVLALLL+PFLW WTAIGTLWF++AH CLPEQGQKWGFLIWLLFSYCALVCIACM
Sbjct: 84  RIVVLSVLALLLFPFLWVWTAIGTLWFKQAHNCLPEQGQKWGFLIWLLFSYCALVCIACM 143

Query: 196 SLGK----------------------VLVDMIRVPDWAFEAAGQETRVMSQDAATY--HP 255
           SLGK                      VLVDMIRVPDWAFEAAG ETRVM QDAATY  HP
Sbjct: 144 SLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDWAFEAAGIETRVMGQDAATYHPHP 203

Query: 256 GLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGC 315
           GLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLEEFH GNEVRGLPCAHNFHVGC
Sbjct: 204 GLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLEEFHEGNEVRGLPCAHNFHVGC 263

Query: 316 IDEWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSASVVTANRYVRSQPSSQSYLVR 375
           IDEWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSASVVTAN Y+ S P SQSYLVR
Sbjct: 264 IDEWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSASVVTANSYMISHPLSQSYLVR 323

Query: 376 LQGLLRPVRTENIGAHSDLESASALEAAENGSAYDQTIPERSPVRPMSLSQSTSQV 408
           LQGLLRPVRTEN G H DLESASA+E  ENGSA+DQTIPERSPVRPM LSQST Q+
Sbjct: 324 LQGLLRPVRTENTGTHGDLESASAVEVTENGSAHDQTIPERSPVRPMPLSQSTPQL 379

[Close]
BLAST of Spg021874 vs. TAIR 10
Match: AT3G47990.1 (SUGAR-INSENSITIVE 3 )

HSP 1 Score: 459.9 bits (1182), Expect = 2.1e-129
Identity = 226/346 (65.32%), Postives = 262/346 (75.72%), Query Frame = 0 

Query: 76  VIVSINWKKYHTCVHPLHIWIVVDYTTVFVFRLLMFVDNGLAAGMGLDFGWQQRYARFCG 135
           +IV++NW +Y  C +PLHIWIVVDYTTVF+FR+ MFVDNGLA+G+GLDFG QQR A FCG
Sbjct: 24  IIVAVNWNRYRACEYPLHIWIVVDYTTVFIFRVFMFVDNGLASGLGLDFGSQQRNAMFCG 83

Query: 136 RIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQGQKWGFLIWLLFSYCALVCIACM 195
           R+VVLSVL+LLLYPFLWAWT IGT WF ++  CLPE+GQKWGFLIWL+FSYC L+CIA +
Sbjct: 84  RVVVLSVLSLLLYPFLWAWTVIGTQWFTKSKTCLPEEGQKWGFLIWLMFSYCGLLCIAFI 143

Query: 196 SLGK----------------------VLVDMIRVPDWAFEAAGQETRVMSQDAATYHPGL 255
            +GK                      +LVDMIRVPDWAFEAAGQE R +SQDAATYHPGL
Sbjct: 144 CVGKWLTRRQVHLLRAQQGIPISEFGILVDMIRVPDWAFEAAGQEMRGISQDAATYHPGL 203

Query: 256 YLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCID 315
           YLTPAQ EAVEALI+ELPKFRLKAVP DC EC ICLEEFH+G+EVRGLPCAHNFHV CID
Sbjct: 204 YLTPAQTEAVEALIQELPKFRLKAVPDDCGECLICLEEFHIGHEVRGLPCAHNFHVECID 263

Query: 316 EWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSASVVTAN---RYVRSQPSSQSYLV 375
           +WLRLNVKCPRCRCSVFP+LDLSALSNL+S      + V T     RY+RSQP S+SY +
Sbjct: 264 QWLRLNVKCPRCRCSVFPDLDLSALSNLQSSGTEQHSQVNTETSEARYIRSQPQSESYFL 323

Query: 376 RLQGLLRPVRTENIGAHSDLESASALEAAENGSAYDQTIPERSPVR 397
           R+Q L+ PV T+           +ALE AENG      + + SP R
Sbjct: 324 RVQSLIHPVHTD-----------TALETAENGGV-PPVLTDLSPSR 357

[Close]
BLAST of Spg021874 vs. TAIR 10
Match: AT4G11680.1 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 71.6 bits (174), Expect = 1.6e-12
Identity = 36/107 (33.64%), Postives = 53/107 (49.53%), Query Frame = 0 

Query: 233 LYLTPAQREAVEALIEELPKFRL-------------KAVPTDC--------------SEC 292
           LY    Q  A +  I+++PKFR              + + T+C              +EC
Sbjct: 279 LYAVADQEGASKNDIDQMPKFRFTKTGNVEKLSGKARGIMTECGTDSPIERSLSPEDAEC 338

Query: 293 PICLEEFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPN 313
            ICL E+  G E+R LPC H+FH  CID+WL +N +CP C+ ++  N
Sbjct: 339 CICLCEYEDGVELRELPCNHHFHCTCIDKWLHINSRCPLCKFNILKN 385

[Close]
BLAST of Spg021874 vs. TAIR 10
Match: AT1G60360.1 (RING/U-box superfamily protein )

HSP 1 Score: 70.5 bits (171), Expect = 3.6e-12
Identity = 37/93 (39.78%), Postives = 50/93 (53.76%), Query Frame = 0 

Query: 237 PAQREAVEALIEELPKFRL--KAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCIDE 296
           P    A E  I  LP  ++  + +  D S+C +C+EEF VG +   LPC H +H  CI  
Sbjct: 194 PGPPPASEPTINSLPSVKITPQHLTNDMSQCTVCMEEFIVGGDATELPCKHIYHKDCIVP 253

Query: 297 WLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDR 328
           WLRLN  CP CR  + P ++  A S  RS+  R
Sbjct: 254 WLRLNNSCPICRRDL-PLVNTVAESRERSNPIR 285

[Close]
BLAST of Spg021874 vs. TAIR 10
Match: AT3G61180.1 (RING/U-box superfamily protein )

HSP 1 Score: 70.5 bits (171), Expect = 3.6e-12
Identity = 47/180 (26.11%), Postives = 75/180 (41.67%), Query Frame = 0 

Query: 150 FLWAWTAIGTLWFREAHKCLPEQGQKWGFLIWLLFSYCA------LVCIACMSL-GKVLV 209
           F + W  IG  W     + L +   +   L WL  ++ A      ++C+A  SL G  + 
Sbjct: 194 FSFVWWIIGFYWVTADTEALAQSSPQ---LYWLCVAFLAFDVMFVVICVAVASLIGIAVC 253

Query: 210 DMIRVPDWAFEAAGQETRVMSQDAATYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDC 269
             +        A   +     ++        +LT    E V   I E     +  + T+ 
Sbjct: 254 CCLPCIIAILYALADQEGAPDEEIERLLKFKFLTVKNSEKVNGEIRETQGGIMTGLDTES 313

Query: 270 ----------SECPICLEEFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPN 313
                     +EC ICL  +  G E+R LPC H+FH  C+D+WLR+N  CP C+ ++  N
Sbjct: 314 QTERMLLSEDAECSICLCAYEDGVELRELPCRHHFHSLCVDKWLRINATCPLCKFNILKN 370

[Close]
BLAST of Spg021874 vs. TAIR 10
Match: AT1G63170.1 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 68.6 bits (166), Expect = 1.4e-11
Identity = 24/53 (45.28%), Postives = 36/53 (67.92%), Query Frame = 0 

Query: 257 AVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSV 310
           A+P + +EC ICL  +    E+R LPC H+FH GC+D+WL +N  CP C+ ++
Sbjct: 317 ALPHEDAECCICLSAYEDETELRELPCGHHFHCGCVDKWLYINATCPLCKYNI 369

The following BLAST results are available for this feature:
BLAST of Spg021874 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_008453098.11.0e-18190.40PREDICTED: E3 ubiquitin-protein ligase SIS3-like [Cucumis melo][more]
XP_004138113.11.2e-17988.70E3 ubiquitin-protein ligase SIS3 [Cucumis sativus] >KGN63587.1 hypothetical prot... [more]
XP_038880106.14.6e-17988.70E3 ubiquitin-protein ligase SIS3-like [Benincasa hispida][more]
XP_023516682.16.7e-17887.92E3 ubiquitin-protein ligase SIS3-like isoform X1 [Cucurbita pepo subsp. pepo][more]
KAG7023134.12.5e-17783.11E3 ubiquitin-protein ligase SIS3 [Cucurbita argyrosperma subsp. argyrosperma][more]
BLAST of Spg021874 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q8GYT93.0e-12865.32E3 ubiquitin-protein ligase SIS3 OS=Arabidopsis thaliana OX=3702 GN=SIS3 PE=2 SV... [more]
Q93Z922.3e-1133.64E3 ubiquitin-protein ligase At4g11680 OS=Arabidopsis thaliana OX=3702 GN=At4g116... [more]
Q8LDB81.9e-1045.28E3 ubiquitin-protein ligase At1g63170 OS=Arabidopsis thaliana OX=3702 GN=At1g631... [more]
Q9SK922.5e-1042.47E3 ubiquitin-protein ligase ATL15 OS=Arabidopsis thaliana OX=3702 GN=ATL15 PE=1 ... [more]
Q9SL784.3e-1045.45Putative RING-H2 finger protein ATL12 OS=Arabidopsis thaliana OX=3702 GN=ATL12 P... [more]
BLAST of Spg021874 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A1S3BVE94.8e-18290.40E3 ubiquitin-protein ligase SIS3-like OS=Cucumis melo OX=3656 GN=LOC103493914 PE... [more]
A0A0A0LS355.9e-18088.70RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G004990 P... [more]
A0A6J1E7C41.6e-17787.64E3 ubiquitin-protein ligase SIS3-like OS=Cucurbita moschata OX=3662 GN=LOC111430... [more]
A0A6J1JJE61.2e-17586.52E3 ubiquitin-protein ligase SIS3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1F5911.1e-17385.67E3 ubiquitin-protein ligase SIS3-like isoform X1 OS=Cucurbita moschata OX=3662 G... [more]
BLAST of Spg021874 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G47990.12.1e-12965.32SUGAR-INSENSITIVE 3 [more]
AT4G11680.11.6e-1233.64Zinc finger, C3HC4 type (RING finger) family protein [more]
AT1G60360.13.6e-1239.78RING/U-box superfamily protein [more]
AT3G61180.13.6e-1226.11RING/U-box superfamily protein [more]
AT1G63170.11.4e-1145.28Zinc finger, C3HC4 type (RING finger) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 265..305
e-value: 5.4E-7
score: 39.2
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 264..306
e-value: 3.6E-13
score: 49.6
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 265..306
score: 12.622055
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 234..311
e-value: 4.4E-19
score: 69.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 377..407
NoneNo IPR availablePANTHERPTHR47179:SF1E3 UBIQUITIN-PROTEIN LIGASE SIS3coord: 76..385
NoneNo IPR availableCDDcd16474RING-H2_RNF111_likecoord: 264..308
e-value: 2.96322E-18
score: 75.8109
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 246..314
IPR044793E3 ubiquitin-protein ligase SIS3PANTHERPTHR47179E3 UBIQUITIN-PROTEIN LIGASE SIS3coord: 76..385

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg021874.1Spg021874.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
biological_process GO:0010182 sugar mediated signaling pathway
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004842 ubiquitin-protein transferase activity