Spg021747 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg021747 vs. NCBI nr
Match: XP_038878610.1 (polygalacturonase 1 beta-like protein 3 [Benincasa hispida])

HSP 1 Score: 1100.1 bits (2844), Expect = 0.0e+00
Identity = 555/625 (88.80%), Postives = 580/625 (92.80%), Query Frame = 0

Query: 1   MVHIVFFLLLSHLSLSLFHVSFASADATLNASIQFSPFTAKASLARYWNSHVSNDLPKPD 60
           MVHIVFFLLLS LSLS FHVSFASAD       Q +PFTAKASL RYWNSHVSN LP PD
Sbjct: 1   MVHIVFFLLLSQLSLSFFHVSFASADHQ-----QSNPFTAKASLLRYWNSHVSNQLPPPD 60

Query: 61  FLFSKASPLSAVDAAVLAKHAAGGSLYSRFESFCYLANLFCSFDSETEISVPRSKDADFK 120
           FLFSKASPLSAVDAAVLA+HAA GSL  R ESFCY ANLFCSFDSETEISVPR KDADFK
Sbjct: 61  FLFSKASPLSAVDAAVLAQHAARGSLSFRLESFCYSANLFCSFDSETEISVPRLKDADFK 120

Query: 121 LYGNRNFVNYGAARLGGVDSFKNYSDGLNSADNSFKRYGGNSSNHREGFTNYAEDGNVAT 180
           LYGN+NFVNYG ARLGG DSFKNYSDGLN+AD+SFKRYGGNSSNH EGFTNYAEDGNVA 
Sbjct: 121 LYGNKNFVNYGGARLGGTDSFKNYSDGLNTADHSFKRYGGNSSNHEEGFTNYAEDGNVAN 180

Query: 181 SNFVSYAAESKGGSGEFTNYDKLVNVPNLRFTAYDLDSRSHNLSFTSYGNETNSGSQSFT 240
           SNF+SYA ESKGGSGEF NYDKLVNVPNLRFTAYDL+SR+H LSFT YGNETNSGSQ+FT
Sbjct: 181 SNFISYATESKGGSGEFVNYDKLVNVPNLRFTAYDLNSRNHKLSFTGYGNETNSGSQTFT 240

Query: 241 SYGKNGNGVPQEFKSYSESSNILTSTFTGYGESGKTANDSFKAYGLSSNNPHTNFKNYGN 300
           SYGK GNGVPQEF SYS+ SNILTSTFTGYGESGKTANDSFK YG+S NNPHTNFKNYG 
Sbjct: 241 SYGKKGNGVPQEFSSYSDGSNILTSTFTGYGESGKTANDSFKGYGVSGNNPHTNFKNYGK 300

Query: 301 GGNSQIDTFSNYRNGANVGDDSFQSYAKGSNSGRVNFANYGKSFNHGNDTFKEYGRGSIG 360
           GGNS+IDTFSNYRNGANVGDDSFQSYAK SNSGRVNFANYGKSFNHGND+F EYG+GSIG
Sbjct: 301 GGNSEIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFANYGKSFNHGNDSFTEYGKGSIG 360

Query: 361 KTTVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGINVNRWVEPGKFFRESELKQGN 420
           +TTVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGI+VN+ VEPGKFFRESELKQGN
Sbjct: 361 RTTVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGIHVNKLVEPGKFFRESELKQGN 420

Query: 421 VMVMPDIRDKMPPRSFLPRPILVNLPFSSSSLNQTKQIFHARDGSTMERVITNALAECER 480
           VMVMPDIRDKMPPRSFLPRPILVNLPFSSS +++ K+IFHARDGSTMERVITNAL ECER
Sbjct: 421 VMVMPDIRDKMPPRSFLPRPILVNLPFSSSRISEAKEIFHARDGSTMERVITNALGECER 480

Query: 481 APSRSETKRCVGSAEDMIDFATAVLGRNVVARTTANVDGSTEKVEIGTVKGINGGELTRS 540
           APS  ETKRCVGSAEDMIDFAT VLGR VVARTTANV+GS EKVEIGTVKGINGG+LT S
Sbjct: 481 APSPGETKRCVGSAEDMIDFATTVLGRRVVARTTANVNGSREKVEIGTVKGINGGDLTSS 540

Query: 541 VSCHQSLYPYLLYYCHSVPKVRIYEADILKLKTKEKINRGVAICHMDTSSWGPEHGAFVA 600
           VSCHQSLYPYLLYYCHSVPKVRIYEADIL+LKTKEKINRGVAICHMDTSSWGPEHGAFVA
Sbjct: 541 VSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTKEKINRGVAICHMDTSSWGPEHGAFVA 600

Query: 601 LGSGPGRIEVCHWIFENDMSWSVAD 626
           LGSGPG+IEVCHWIFENDMSWSVAD
Sbjct: 601 LGSGPGQIEVCHWIFENDMSWSVAD 620

BLAST of Spg021747 vs. NCBI nr
Match: KAG6589452.1 (Polygalacturonase 1 beta-like protein 3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023135.1 Polygalacturonase 1 beta-like protein 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1096.6 bits (2835), Expect = 0.0e+00
Identity = 554/626 (88.50%), Postives = 588/626 (93.93%), Query Frame = 0

Query: 1   MVHIVFFLLLSHLSLSLFHVSFASAD-ATLNASIQFSPFTAKASLARYWNSHVSNDLPKP 60
           MVHIVFFLLLS LSLSL HVSF SAD AT NASIQF+PFTAKASL RYWN+HVSN LPKP
Sbjct: 6   MVHIVFFLLLSQLSLSLLHVSFVSADQATFNASIQFNPFTAKASLVRYWNTHVSNQLPKP 65

Query: 61  DFLFSKASPLSAVDAAVLAKHAAGGSLYSRFESFCYLANLFCSFDSETEISVPRSKDADF 120
           DFLF+KASPLSAVDAAV AKHAA GSL SR E+FCY ANLFCSFDS+ E S+PR KDADF
Sbjct: 66  DFLFAKASPLSAVDAAVFAKHAALGSLTSRLETFCYSANLFCSFDSQ-ETSIPRFKDADF 125

Query: 121 KLYGNRNFVNYGAARLGGVDSFKNYSDGLNSADNSFKRYGGNSSNHREGFTNYAEDGNVA 180
           KLYGN+NF NYGAAR+GG DSFKNYSDGLN+AD+SFKRYGGNSSNH+EGF NYAEDGNVA
Sbjct: 126 KLYGNKNFNNYGAARIGGADSFKNYSDGLNTADHSFKRYGGNSSNHKEGFMNYAEDGNVA 185

Query: 181 TSNFVSYAAESKGGSGEFTNYDKLVNVPNLRFTAYDLDSRSHNLSFTSYGNETNSGSQSF 240
            +NF+SYAAESKGGSGEF NYDK+VNVPNLRFTAYDLDSR+H LSFTSYGNETNSGSQSF
Sbjct: 186 NNNFISYAAESKGGSGEFVNYDKIVNVPNLRFTAYDLDSRAHKLSFTSYGNETNSGSQSF 245

Query: 241 TSYGKNGNGVPQEFKSYSESSNILTSTFTGYGESGKTANDSFKAYGLSSNNPHTNFKNYG 300
           TSYGKNGNGVPQEF SYS+ SNILTSTFTGYGESGK+ANDSFKAYGLS NNPH+NFKNYG
Sbjct: 246 TSYGKNGNGVPQEFSSYSDGSNILTSTFTGYGESGKSANDSFKAYGLSGNNPHSNFKNYG 305

Query: 301 NGGNSQIDTFSNYRNGANVGDDSFQSYAKGSNSGRVNFANYGKSFNHGNDTFKEYGRGSI 360
            GG SQIDTFSNYRNGANVGDDSFQSYAK SNSGRVNFA+YG+SFNHGND+FKEYG+GSI
Sbjct: 306 KGGKSQIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFASYGQSFNHGNDSFKEYGKGSI 365

Query: 361 GKTTVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGINVNRWVEPGKFFRESELKQG 420
           G+TTVGF+SYSVDRNFTTYAQKGVSFSEYS    ATGSGINVNRWVEPGKFFRESELKQG
Sbjct: 366 GQTTVGFRSYSVDRNFTTYAQKGVSFSEYS----ATGSGINVNRWVEPGKFFRESELKQG 425

Query: 421 NVMVMPDIRDKMPPRSFLPRPILVNLPFSSSSLNQTKQIFHARDGSTMERVITNALAECE 480
           NVMVMPDIRDKMPPRSFLPR ILVN+PFSSS +++TKQIFHAR+GSTME VIT+ALAECE
Sbjct: 426 NVMVMPDIRDKMPPRSFLPRAILVNIPFSSSKISETKQIFHARNGSTMEHVITSALAECE 485

Query: 481 RAPSRSETKRCVGSAEDMIDFATAVLGRNVVARTTANVDGSTEKVEIGTVKGINGGELTR 540
           RAPS  ETKRCVGSAEDMIDFATAVLGRNVVARTTA+V+GSTEKVEIG VKGINGGELTR
Sbjct: 486 RAPSMGETKRCVGSAEDMIDFATAVLGRNVVARTTASVNGSTEKVEIGEVKGINGGELTR 545

Query: 541 SVSCHQSLYPYLLYYCHSVPKVRIYEADILKLKTKEKINRGVAICHMDTSSWGPEHGAFV 600
           SVSCHQSLYPYLLYYCHSVPKVRIYEADIL+LKT EKINRGVAICHMDTSSWGPEHGAFV
Sbjct: 546 SVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTNEKINRGVAICHMDTSSWGPEHGAFV 605

Query: 601 ALGSGPGRIEVCHWIFENDMSWSVAD 626
           ALGSGPG+IEVCHWIFENDMSWSVAD
Sbjct: 606 ALGSGPGQIEVCHWIFENDMSWSVAD 626

BLAST of Spg021747 vs. NCBI nr
Match: XP_022988353.1 (polygalacturonase 1 beta-like protein 3 [Cucurbita maxima])

HSP 1 Score: 1095.9 bits (2833), Expect = 0.0e+00
Identity = 555/626 (88.66%), Postives = 588/626 (93.93%), Query Frame = 0

Query: 1   MVHIVFFLLLSHLSLSLFHVSFASAD-ATLNASIQFSPFTAKASLARYWNSHVSNDLPKP 60
           MVHIVFFLLLS LSLSL HVSF SAD AT NASI F+PFTAKASL RYWN+HVSN LPKP
Sbjct: 6   MVHIVFFLLLSQLSLSLLHVSFVSADQATFNASIHFNPFTAKASLVRYWNTHVSNQLPKP 65

Query: 61  DFLFSKASPLSAVDAAVLAKHAAGGSLYSRFESFCYLANLFCSFDSETEISVPRSKDADF 120
           DFLF KASPLSAVDAAV AKHAA GSL SR E+FCY ANLFCSFDS+ E SVPR KDADF
Sbjct: 66  DFLFVKASPLSAVDAAVFAKHAALGSLTSRLETFCYSANLFCSFDSQ-ETSVPRFKDADF 125

Query: 121 KLYGNRNFVNYGAARLGGVDSFKNYSDGLNSADNSFKRYGGNSSNHREGFTNYAEDGNVA 180
           KLYGN+NF NYGAAR+GG DSFKNYSDGLN+AD+SFKRYGGNSSNH+EGFTNYAEDGNVA
Sbjct: 126 KLYGNKNFNNYGAARIGGADSFKNYSDGLNTADHSFKRYGGNSSNHKEGFTNYAEDGNVA 185

Query: 181 TSNFVSYAAESKGGSGEFTNYDKLVNVPNLRFTAYDLDSRSHNLSFTSYGNETNSGSQSF 240
            +NF+SYAAESKGGSGEF NYDK+VNVPNLRFTAYDLDSR+H LSFTSYGNETNSGSQSF
Sbjct: 186 NNNFISYAAESKGGSGEFVNYDKIVNVPNLRFTAYDLDSRAHKLSFTSYGNETNSGSQSF 245

Query: 241 TSYGKNGNGVPQEFKSYSESSNILTSTFTGYGESGKTANDSFKAYGLSSNNPHTNFKNYG 300
           TSYGKNGNGVPQEF SYS+ SNILTSTFTGYGESGK+ANDSFKAYGLSSNNPH+NFKNYG
Sbjct: 246 TSYGKNGNGVPQEFSSYSDGSNILTSTFTGYGESGKSANDSFKAYGLSSNNPHSNFKNYG 305

Query: 301 NGGNSQIDTFSNYRNGANVGDDSFQSYAKGSNSGRVNFANYGKSFNHGNDTFKEYGRGSI 360
            GG SQIDTFSNYRNGANVGDDSFQSYAK SNSGRVNFA+YG+SFNHGND+FKEY +GSI
Sbjct: 306 KGGKSQIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFASYGQSFNHGNDSFKEYAKGSI 365

Query: 361 GKTTVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGINVNRWVEPGKFFRESELKQG 420
           G+TTVGF+SYSVDRNFTTYAQKGVSFSEYS    ATGSGINVNRWVEPGKFFRESELKQG
Sbjct: 366 GQTTVGFRSYSVDRNFTTYAQKGVSFSEYS----ATGSGINVNRWVEPGKFFRESELKQG 425

Query: 421 NVMVMPDIRDKMPPRSFLPRPILVNLPFSSSSLNQTKQIFHARDGSTMERVITNALAECE 480
           NVMVMPDIRDKMPPRSFLPR ILVN+PFSSS +++TKQIFHAR+GSTMERVIT+ALAECE
Sbjct: 426 NVMVMPDIRDKMPPRSFLPRAILVNIPFSSSKISETKQIFHARNGSTMERVITSALAECE 485

Query: 481 RAPSRSETKRCVGSAEDMIDFATAVLGRNVVARTTANVDGSTEKVEIGTVKGINGGELTR 540
           RAPS  ETKRCVGSAEDMIDFATAVLGRNVVARTTA+V+GSTEKVEIG VKGINGGELTR
Sbjct: 486 RAPSMGETKRCVGSAEDMIDFATAVLGRNVVARTTASVNGSTEKVEIGEVKGINGGELTR 545

Query: 541 SVSCHQSLYPYLLYYCHSVPKVRIYEADILKLKTKEKINRGVAICHMDTSSWGPEHGAFV 600
           SVSCHQSLYPYLLYYCHSVPKVRIYEADIL+LKT EKINRGVAICHMDTSSWGPEHGAFV
Sbjct: 546 SVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTNEKINRGVAICHMDTSSWGPEHGAFV 605

Query: 601 ALGSGPGRIEVCHWIFENDMSWSVAD 626
           ALGSGPG+IEVCHWIFEND+SWSVAD
Sbjct: 606 ALGSGPGQIEVCHWIFENDVSWSVAD 626

BLAST of Spg021747 vs. NCBI nr
Match: XP_022921994.1 (polygalacturonase 1 beta-like protein 3 [Cucurbita moschata])

HSP 1 Score: 1095.5 bits (2832), Expect = 0.0e+00
Identity = 554/626 (88.50%), Postives = 587/626 (93.77%), Query Frame = 0

Query: 1   MVHIVFFLLLSHLSLSLFHVSFASAD-ATLNASIQFSPFTAKASLARYWNSHVSNDLPKP 60
           MVHIVFFLLLS LSLSL HVSF SAD AT NASIQF+PFTAKASL RYWN+HVSN LPKP
Sbjct: 6   MVHIVFFLLLSQLSLSLLHVSFVSADQATFNASIQFNPFTAKASLVRYWNTHVSNQLPKP 65

Query: 61  DFLFSKASPLSAVDAAVLAKHAAGGSLYSRFESFCYLANLFCSFDSETEISVPRSKDADF 120
           DFLF+KASPLSAVDAAV AKHAA GSL SR E+FCY ANLFCSFDS+ E SVPR KDADF
Sbjct: 66  DFLFAKASPLSAVDAAVFAKHAALGSLTSRLETFCYSANLFCSFDSQ-ETSVPRFKDADF 125

Query: 121 KLYGNRNFVNYGAARLGGVDSFKNYSDGLNSADNSFKRYGGNSSNHREGFTNYAEDGNVA 180
           KLYGN+NF NYGAAR+GG DSFKNYSDGLN+AD+SFKRYGGNSSNH+EGFTNYAEDGNVA
Sbjct: 126 KLYGNKNFNNYGAARIGGADSFKNYSDGLNTADHSFKRYGGNSSNHKEGFTNYAEDGNVA 185

Query: 181 TSNFVSYAAESKGGSGEFTNYDKLVNVPNLRFTAYDLDSRSHNLSFTSYGNETNSGSQSF 240
            +NF+SYAAESKGGSGEF NYDK+VNVPNLRFTAYDLDSR+H LSFTSYGNETNSGSQSF
Sbjct: 186 NNNFISYAAESKGGSGEFVNYDKIVNVPNLRFTAYDLDSRAHKLSFTSYGNETNSGSQSF 245

Query: 241 TSYGKNGNGVPQEFKSYSESSNILTSTFTGYGESGKTANDSFKAYGLSSNNPHTNFKNYG 300
           TSYGKNGNGVPQEF SYS+ SNILTSTFTGYGESGK+ANDSFKAYGLS NNPH+NFKNYG
Sbjct: 246 TSYGKNGNGVPQEFSSYSDGSNILTSTFTGYGESGKSANDSFKAYGLSGNNPHSNFKNYG 305

Query: 301 NGGNSQIDTFSNYRNGANVGDDSFQSYAKGSNSGRVNFANYGKSFNHGNDTFKEYGRGSI 360
            GG SQIDTFSNYRNGANVGDDSFQSYAK SNSGRVNFA+YG+SFNHGND+FKEYG+GSI
Sbjct: 306 KGGKSQIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFASYGQSFNHGNDSFKEYGKGSI 365

Query: 361 GKTTVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGINVNRWVEPGKFFRESELKQG 420
           G+TTVGF+SYSVDRNFTTYAQKGVSFSEYS    ATGSGINVNRWVEPGKFFRESELKQG
Sbjct: 366 GQTTVGFRSYSVDRNFTTYAQKGVSFSEYS----ATGSGINVNRWVEPGKFFRESELKQG 425

Query: 421 NVMVMPDIRDKMPPRSFLPRPILVNLPFSSSSLNQTKQIFHARDGSTMERVITNALAECE 480
           NVMVMPDIRDKMPPRSFLPR ILVN+PFSSS +++TKQIFHAR+GSTME VIT+ALAECE
Sbjct: 426 NVMVMPDIRDKMPPRSFLPRAILVNIPFSSSKISETKQIFHARNGSTMEHVITSALAECE 485

Query: 481 RAPSRSETKRCVGSAEDMIDFATAVLGRNVVARTTANVDGSTEKVEIGTVKGINGGELTR 540
           RAPS  ETKRCVGSAEDMIDFATAVLGRNVV RTTA+V+GS EKVEIG VKGINGGELTR
Sbjct: 486 RAPSMGETKRCVGSAEDMIDFATAVLGRNVVVRTTASVNGSKEKVEIGEVKGINGGELTR 545

Query: 541 SVSCHQSLYPYLLYYCHSVPKVRIYEADILKLKTKEKINRGVAICHMDTSSWGPEHGAFV 600
           SVSCHQSLYPYLLYYCHSVPKVRIYEADIL+LKT EKINRGVAICHMDTSSWGPEHGAFV
Sbjct: 546 SVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTNEKINRGVAICHMDTSSWGPEHGAFV 605

Query: 601 ALGSGPGRIEVCHWIFENDMSWSVAD 626
           ALGSGPG+IEVCHWIFENDMSWSVAD
Sbjct: 606 ALGSGPGQIEVCHWIFENDMSWSVAD 626

BLAST of Spg021747 vs. NCBI nr
Match: XP_023516985.1 (polygalacturonase 1 beta-like protein 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1090.9 bits (2820), Expect = 0.0e+00
Identity = 551/626 (88.02%), Postives = 586/626 (93.61%), Query Frame = 0

Query: 1   MVHIVFFLLLSHLSLSLFHVSFASAD-ATLNASIQFSPFTAKASLARYWNSHVSNDLPKP 60
           MVHIVFFLLLS LSLSL HVSF SAD AT NASIQF+PFTAKASL RYWN+HVSN LPKP
Sbjct: 6   MVHIVFFLLLSQLSLSLLHVSFVSADQATFNASIQFNPFTAKASLVRYWNTHVSNQLPKP 65

Query: 61  DFLFSKASPLSAVDAAVLAKHAAGGSLYSRFESFCYLANLFCSFDSETEISVPRSKDADF 120
           DFLF+KASPLS VDAAV AKHAA GSL SR E+FCY ANLFCSFDS+ E SVPR KDADF
Sbjct: 66  DFLFAKASPLSVVDAAVFAKHAALGSLTSRLETFCYSANLFCSFDSQ-ETSVPRFKDADF 125

Query: 121 KLYGNRNFVNYGAARLGGVDSFKNYSDGLNSADNSFKRYGGNSSNHREGFTNYAEDGNVA 180
           KLYGN+NF NYGAAR+GG DSFKNYSDGLN+AD+SFKRY GNSSNH+EGFTNYAEDGNVA
Sbjct: 126 KLYGNKNFNNYGAARIGGADSFKNYSDGLNTADHSFKRYSGNSSNHKEGFTNYAEDGNVA 185

Query: 181 TSNFVSYAAESKGGSGEFTNYDKLVNVPNLRFTAYDLDSRSHNLSFTSYGNETNSGSQSF 240
            +NF+SYAAESKGGSGEF NYDK+VNVPNLRFTAYDLDSR+H LSFTSYGNETNSGSQSF
Sbjct: 186 NNNFISYAAESKGGSGEFVNYDKIVNVPNLRFTAYDLDSRAHKLSFTSYGNETNSGSQSF 245

Query: 241 TSYGKNGNGVPQEFKSYSESSNILTSTFTGYGESGKTANDSFKAYGLSSNNPHTNFKNYG 300
           TSYGKNGNGVPQEF SYS+ SNILTSTF+GYGESGK+ANDSFKAYGLS NNPH+NFKNYG
Sbjct: 246 TSYGKNGNGVPQEFSSYSDGSNILTSTFSGYGESGKSANDSFKAYGLSGNNPHSNFKNYG 305

Query: 301 NGGNSQIDTFSNYRNGANVGDDSFQSYAKGSNSGRVNFANYGKSFNHGNDTFKEYGRGSI 360
            GG SQIDTFSNYRNGANVGDDSFQSYAK SNSGRVNFA+YG+SFNHGND+FKEYG+GSI
Sbjct: 306 KGGKSQIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFASYGQSFNHGNDSFKEYGKGSI 365

Query: 361 GKTTVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGINVNRWVEPGKFFRESELKQG 420
           G+TTVGF+SYSVDRNFTTYAQKGVSFSEYS    ATGSGINVNRWVEPGKFFRESELKQG
Sbjct: 366 GQTTVGFRSYSVDRNFTTYAQKGVSFSEYS----ATGSGINVNRWVEPGKFFRESELKQG 425

Query: 421 NVMVMPDIRDKMPPRSFLPRPILVNLPFSSSSLNQTKQIFHARDGSTMERVITNALAECE 480
           NVMVMPDIRDKMPPRSFLPR ILVN+PFSSS +++TKQIFHAR+GSTME VIT+ LAECE
Sbjct: 426 NVMVMPDIRDKMPPRSFLPRAILVNIPFSSSKISETKQIFHARNGSTMEHVITSTLAECE 485

Query: 481 RAPSRSETKRCVGSAEDMIDFATAVLGRNVVARTTANVDGSTEKVEIGTVKGINGGELTR 540
           RAPS  ETKRCVGSAEDMIDFATAVLGRNVVARTTA+V+GSTEKVEIG VKGINGGELTR
Sbjct: 486 RAPSMGETKRCVGSAEDMIDFATAVLGRNVVARTTASVNGSTEKVEIGEVKGINGGELTR 545

Query: 541 SVSCHQSLYPYLLYYCHSVPKVRIYEADILKLKTKEKINRGVAICHMDTSSWGPEHGAFV 600
           SVSCHQSLYPYLLYYCHSVPKVRIYEADIL+LKT EKINRGVAICHMDTSSWGP+HGAFV
Sbjct: 546 SVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTNEKINRGVAICHMDTSSWGPKHGAFV 605

Query: 601 ALGSGPGRIEVCHWIFENDMSWSVAD 626
           ALGSGPG+IEVCHWIFENDMSWSVAD
Sbjct: 606 ALGSGPGQIEVCHWIFENDMSWSVAD 626

BLAST of Spg021747 vs. ExPASy Swiss-Prot
Match: P92990 (Polygalacturonase 1 beta-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=PGL3 PE=2 SV=2)

HSP 1 Score: 703.0 bits (1813), Expect = 3.1e-201
Identity = 366/624 (58.65%), Postives = 446/624 (71.47%), Query Frame = 0

Query: 7   FLLLSHLSLSLFHVSFASADATLNA-SIQFSPFTAKASLARYWNSHVSNDLPKPDFLFSK 66
           FLLL   S  LF+V            S + +PFT KASL RYWN  +    P+ +FL SK
Sbjct: 5   FLLLQSFSFFLFNVVIVGGRTFGGGFSAEENPFTPKASLVRYWNKEIRGQSPRSEFLISK 64

Query: 67  ASPLSAVDAAVLAKHAAGGSLYSRFESFCYLANLFCSFDSETEISVPRSKDADFKLYGNR 126
           ASPL+AVD+A  +K AA  SL +RF  FC  ANLFC  D    +      D  F +Y  +
Sbjct: 65  ASPLNAVDSATFSKLAAANSLPTRFPDFCSAANLFCFPDLGASLE-KHDDDVKFSVYDQK 124

Query: 127 NFVNYGAARLGGVDSFKNYSDGLNSADNSFKRYGGNSSNHREGFTNYAEDGNVATSNFVS 186
           NF NYG AR GG DSFKNYS   N   +SF+RY  N++ H + FT Y E+ NV    F S
Sbjct: 125 NFTNYGNARAGGADSFKNYSKDGNVVTDSFRRYSRNAAGHDDKFTVYGENSNVVEEGFNS 184

Query: 187 YAAESKGGSGEFTNYDKLVNVPNLRFTAYDLDSRSHNLSFTSYGNETNSGS-QSFTSYGK 246
           Y     GG+G+FTNY   VN P  RFTAY       + +F +Y +E N+G+ QSFTSYGK
Sbjct: 185 YGTFGTGGAGDFTNYQNNVNNPTSRFTAYSDGGNGRSQTFKTYTHEANAGNGQSFTSYGK 244

Query: 247 NGNGVPQEFKSYSESSNILTSTFTGYGESGKTANDSFKAYGLSSNNPHTNFKNYGNGGNS 306
           NGNGVP EF SY  SSN++ S F+ YGESG  AND+F +YG   N P  NF NYG  GN+
Sbjct: 245 NGNGVPNEFTSYGVSSNVIGSGFSNYGESGNAANDTFTSYGSDGNVPQNNFNNYGASGNA 304

Query: 307 QIDTFSNYRNGANVGDDSFQSYAKGSNSGRVNFANYGKSFNHGNDTFKEYGRGSIGKTTV 366
            +DTF+NYR+ ANVGDDSF SYAK SNS +VNF NYG+SFN G++TF  YG+G+ G + +
Sbjct: 305 AVDTFANYRDKANVGDDSFSSYAKDSNSEKVNFVNYGQSFNPGSETFTGYGKGAEG-SKL 364

Query: 367 GFKSYSVDRNFTTYAQKGVSFSEYSNFTT---ATGSGINVNRWVEPGKFFRESELKQGNV 426
            FK+Y+ +  F  YA+KGV+F++Y+  TT     G G  VN+W+EPGKFFRES LK+G V
Sbjct: 365 SFKTYTPNSTFKDYAKKGVAFAKYNVSTTTANTVGDGKTVNKWIEPGKFFRESSLKEGTV 424

Query: 427 MVMPDIRDKMPPRSFLPRPILVNLPFSSSSLNQTKQIFHARDGSTMERVITNALAECERA 486
           + MPDI+DKMP RSFLPR I+  LPFS+S L + K+IFHA + STM  +IT+A+ ECER 
Sbjct: 425 IPMPDIKDKMPKRSFLPRSIITKLPFSTSKLGEIKRIFHAVENSTMGGIITDAVTECERP 484

Query: 487 PSRSETKRCVGSAEDMIDFATAVLGRNVVARTTANVDGSTEKVEIGTVKGINGGELTRSV 546
           PS  ETKRCVGSAEDMIDFAT+VLGR+VV RTT NV GS EKV IG V GINGG+LT++V
Sbjct: 485 PSVGETKRCVGSAEDMIDFATSVLGRSVVLRTTENVAGSKEKVVIGKVNGINGGKLTKAV 544

Query: 547 SCHQSLYPYLLYYCHSVPKVRIYEADILKLKTKEKINRGVAICHMDTSSWGPEHGAFVAL 606
           SCHQSLYPYLLYYCHSVPKVR+YEAD+L+L +K+KIN G+AICHMDTSSWGP HGAF+AL
Sbjct: 545 SCHQSLYPYLLYYCHSVPKVRVYEADLLELNSKKKINHGIAICHMDTSSWGPSHGAFLAL 604

Query: 607 GSGPGRIEVCHWIFENDMSWSVAD 626
           GS PGRIEVCHWIFENDM+W++AD
Sbjct: 605 GSKPGRIEVCHWIFENDMNWAIAD 626

BLAST of Spg021747 vs. ExPASy Swiss-Prot
Match: P92982 (Polygalacturonase 1 beta-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=PGL1 PE=2 SV=2)

HSP 1 Score: 667.5 bits (1721), Expect = 1.4e-190
Identity = 349/623 (56.02%), Postives = 433/623 (69.50%), Query Frame = 0

Query: 6   FFLLLSHLSLSLFHVSFASADATLNA--SIQFSPFTAKASLARYWNSHVSNDLPKPDFLF 65
           F  LL  LS  L+HV  A  +   N   S + +PFT KASL RYWN  + +  P   FL 
Sbjct: 5   FVFLLPFLS-RLYHVVIAGGERLTNGGFSPEINPFTPKASLVRYWNKQIHHQSPGSAFLL 64

Query: 66  SKASPLSAVDAAVLAKHAAGGSLYSRFESFCYLANLFCSFDSETEISVPRSKDADFKLYG 125
           SKASPL+AV +A  AK A+  +L  R   FC  ANLFC  D    +    S D  F +Y 
Sbjct: 65  SKASPLTAVASATFAKLASENALPDRLPDFCSAANLFCFPDLGPTLE-KHSNDVKFSVYD 124

Query: 126 NRNFVNYGAARLGGVDSFKNYSDGLNSADNSFKRYGGNSSNHREGFTNYAEDGNVATSNF 185
            RNF NYG    GG DSFK YS   N+  +SF+RY  +++ H + FT YA++ NV    F
Sbjct: 125 QRNFTNYGTIHPGGADSFKKYSQNGNAVTDSFRRYSRDAAGHEDKFTGYADNSNVVEEKF 184

Query: 186 VSYAAESKGGSGEFTNYDKLVNVPNLRFTAYDLDSRSHNLSFTSYGNETNSG-SQSFTSY 245
            SY   + GGSG+F NY   VN P  RF AY  +      +F +Y +E NSG  QSFTSY
Sbjct: 185 NSYGTSATGGSGDFKNYQSGVNNPTTRFKAYSDEGNGRAQTFKTYTHEANSGPGQSFTSY 244

Query: 246 GKNGNGVPQEFKSYSESSNILTSTFTGYGESGKTANDSFKAYGLSSNNPHTNFKNYGNGG 305
           GKNGNG P EF SY  SSN++ S F+ YGE+G  AND+FK YG   N P  NFK+YG  G
Sbjct: 245 GKNGNGAPNEFSSYGVSSNVIGSDFSNYGENGNAANDTFKGYGGDGNVPQNNFKSYGASG 304

Query: 306 NSQIDTFSNYRNGANVGDDSFQSYAKGSNSGRVNFANYGKSFNHGNDTFKEYGRGSIGKT 365
           N+ +DTF NYR+ ANVGDDSF SYAK SN  +V+F NYGKS N G+++F  YG+G+ G  
Sbjct: 305 NAAVDTFLNYRDNANVGDDSFSSYAKNSNFEKVDFVNYGKSINPGSESFSGYGKGAEG-N 364

Query: 366 TVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGINVNRWVEPGKFFRESELKQGNVM 425
            + FK+Y+ +  F  Y + GV F++Y+   ++ G G  VN+WVEPGKFFRES LK+G ++
Sbjct: 365 KIDFKTYTQNSTFKDYTKTGVEFAKYNR--SSLGGGKTVNKWVEPGKFFRESMLKEGTLI 424

Query: 426 VMPDIRDKMPPRSFLPRPILVNLPFSSSSLNQTKQIFHARDGSTMERVITNALAECERAP 485
            MPDI+DKMP RSFLPR I+  LPFS+S + + K++FHA D STME +IT+A+ ECER P
Sbjct: 425 WMPDIKDKMPKRSFLPRSIVSKLPFSTSKIAEIKRVFHANDNSTMEGIITDAVRECERPP 484

Query: 486 SRSETKRCVGSAEDMIDFATAVLGRNVVARTTANVDGSTEKVEIGTVKGINGGELTRSVS 545
           + SETKRCVGSAEDMIDFAT+VLGR+VV RTT +V GS EKV IG V GINGG +T+SVS
Sbjct: 485 TVSETKRCVGSAEDMIDFATSVLGRSVVLRTTESVAGSKEKVMIGKVNGINGGRVTKSVS 544

Query: 546 CHQSLYPYLLYYCHSVPKVRIYEADILKLKTKEKINRGVAICHMDTSSWGPEHGAFVALG 605
           CHQSLYPYLLYYCHSVPKVR+YE+D+L  K+K KIN G+AICHMDTS+WG  HGAF+ LG
Sbjct: 545 CHQSLYPYLLYYCHSVPKVRVYESDLLDPKSKAKINHGIAICHMDTSAWGANHGAFMLLG 604

Query: 606 SGPGRIEVCHWIFENDMSWSVAD 626
           S PG+IEVCHWIFENDM+W++AD
Sbjct: 605 SRPGQIEVCHWIFENDMNWAIAD 622

BLAST of Spg021747 vs. ExPASy Swiss-Prot
Match: O80760 (Polygalacturonase 1 beta-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=PGL2 PE=2 SV=1)

HSP 1 Score: 664.8 bits (1714), Expect = 9.3e-190
Identity = 340/608 (55.92%), Postives = 428/608 (70.39%), Query Frame = 0

Query: 19  HVSFASADATL-NASIQFSPFTAKASLARYWNSHVSNDLPKPDFLFSKASPLSAVDAAVL 78
           +V FA A  T  N +   +PFT KASL RYWN+H++ D PKP F  SKASPL+AVD+   
Sbjct: 20  NVHFAGAKQTAGNITPSENPFTPKASLIRYWNNHINGDSPKPSFFLSKASPLTAVDSTRF 79

Query: 79  AKHAAGGSLYSRFESFCYLANLFCSFDSETEISVPRSKDADFKLYGNRNFVNYGAARLGG 138
           A  A+  +L +    FC  A LFC  +           D +F  Y  +NF NYG+ RL G
Sbjct: 80  ASLASNHALNTHHSDFCSAAKLFCFPELAAHSLEKHGDDVNFAAYSGKNFTNYGSDRLSG 139

Query: 139 VDSFKNYSDGLNSADNSFKRYGGNSSNHREGFTNYAEDGNVATSNFVSYAAESKGGSGEF 198
            DSFKNYS G N A +SF+RY  NS+ H +GFTNYA + NVA  +F +YA  + GGSGEF
Sbjct: 140 ADSFKNYSGGDNIAVDSFRRYSRNSAGHDDGFTNYAGEVNVADQSFTTYATGTTGGSGEF 199

Query: 199 TNYDKLVNVPNLRFTAYDLDSRSHNLSFTSYGNETNSGSQSFTSYGKNGNGVPQEFKSYS 258
           TNY+   N PN RFT+Y   +   + +FT+Y    N+G QSFTSY KNGNG P EF  Y 
Sbjct: 200 TNYNTDANEPNGRFTSYSDKANGRSQTFTTYSENGNTGYQSFTSYSKNGNGAPNEFSGYG 259

Query: 259 ESSNILTSTFTGYGESGKTANDSFKAYGLSSNNPHTNFKNYGNGGNSQIDTFSNYRNGAN 318
             SN++ + FT YGES   ANDSF +YG + N P   FK YG+GGN  +  F NYR+ +N
Sbjct: 260 TGSNVVNTGFTKYGESANGANDSFTSYGENGNVPVNEFKGYGDGGNGAVYGFKNYRDQSN 319

Query: 319 VGDDSFQSYAKGSNSGRVNFANYGKSFNHGNDTFKEYGRGSIGKTTVGFKSYSVDRNFTT 378
           +G DSF SYAK SN+ +VNF NYGKSFN G+D F  YG+ ++G   V FK+Y   ++F  
Sbjct: 320 IGVDSFSSYAKNSNNEKVNFVNYGKSFNLGSDNFTGYGQDNVG-GNVSFKTYGQGQSFKV 379

Query: 379 YAQKGVSFSEYSNFTTATGSGINVNRWVEPGKFFRESELKQGNVMVMPDIRDKMPPRSFL 438
           Y + GV F+ YSN    + +G  VN+WVE GKFFRE+ LK+G +M MPDI+DKMP R+FL
Sbjct: 380 YTKDGVVFARYSN--NVSSNGKTVNKWVEEGKFFREAMLKEGTLMQMPDIKDKMPKRTFL 439

Query: 439 PRPILVNLPFSSSSLNQTKQIFHARDGSTMERVITNALAECERAPSRSETKRCVGSAEDM 498
           PR I+ NLPFSSS++ +  ++F A + S+M  +I++A++ECER  S  ETKRCVGSAEDM
Sbjct: 440 PRNIVKNLPFSSSTIGEIWRVFGAGENSSMAGIISSAVSECERPASHGETKRCVGSAEDM 499

Query: 499 IDFATAVLGRNVVARTTANVDGSTEKVEIGTVKGINGGELTRSVSCHQSLYPYLLYYCHS 558
           IDFAT+VLGR VV RTT NV GS +KV IG V GINGG++TR+VSCHQSLYPYLLYYCHS
Sbjct: 500 IDFATSVLGRGVVVRTTENVVGSKKKVVIGKVNGINGGDVTRAVSCHQSLYPYLLYYCHS 559

Query: 559 VPKVRIYEADILKLKTKEKINRGVAICHMDTSSWGPEHGAFVALGSGPGRIEVCHWIFEN 618
           VP+VR+YE D+L  K+ EKIN GVAICH+DTS+W P HGAF+ALGSGPG+IEVCHWIFEN
Sbjct: 560 VPRVRVYETDLLDPKSLEKINHGVAICHIDTSAWSPSHGAFLALGSGPGQIEVCHWIFEN 619

Query: 619 DMSWSVAD 626
           DM+W++ D
Sbjct: 620 DMTWNIID 624

BLAST of Spg021747 vs. ExPASy Swiss-Prot
Match: P93217 (Polygalacturonase non-catalytic subunit AroGP2 OS=Solanum lycopersicum OX=4081 GN=GP2 PE=3 SV=1)

HSP 1 Score: 632.5 bits (1630), Expect = 5.1e-180
Identity = 338/630 (53.65%), Postives = 425/630 (67.46%), Query Frame = 0

Query: 6   FFLLLSHLSLSLFHVSFASADATLNASIQFSPFTAKASLARYWNSHVSNDLPKPDFLFSK 65
           + LL+   SLS F++  A      +     +PFT K  + RYWN HVSNDLPKP FL +K
Sbjct: 10  YILLVLLFSLSSFNIVVAK-----DGDESGNPFTPKGYVIRYWNKHVSNDLPKPWFLLNK 69

Query: 66  ASPLSAVDAAVLAKHAAG-GSLYSRFESFCYLANLFCSFDSETEISVPRSKDADFKLYGN 125
           ASPL+A   A   K  A   +L +  +SFC  ANL C+ D    +    + D  F  YGN
Sbjct: 70  ASPLNAAQYATYTKLVADQNALSTHLQSFCSSANLMCAPDLLPSLE-KHTGDIHFTTYGN 129

Query: 126 RNFVNYGAARLG-GVDSFKNYSDGLNSADNSFKRYGGNSSNHREGFTNYAEDGNVATSNF 185
           +NF NYG    G GV++FKNYS+  +++ NSF+RYG  S    + F NYA DGNV   +F
Sbjct: 130 KNFTNYGTNEPGIGVNTFKNYSE--DASVNSFRRYGRGSPRDNK-FDNYAPDGNVIDQSF 189

Query: 186 VSYAAESKGGSGEFTNYDKLVNVPNLRFTAYDLDSRSHNLSFTSYGNETNSGSQSFTSYG 245
            SY+  + GGSG+FTNY    NVP+LRFTAY          F +Y  + NSG QSF SYG
Sbjct: 190 NSYSTNTPGGSGQFTNYAPNTNVPDLRFTAYSDQGTGGEQEFKTYLEQGNSGGQSFKSYG 249

Query: 246 KNGNGVPQEFKSYSESSNILTSTFTGYGESGKTANDSFKAYGLSSNNPHTNFKNYGNGGN 305
           KNGNG   +F SY   +N+  STF  YG++    N +F +Y  + NNP  NFKNYG GGN
Sbjct: 250 KNGNGADSKFTSYGNETNVAASTFKNYGQNANGENQNFTSYSTNGNNPQNNFKNYGVGGN 309

Query: 306 SQIDTFSNYRNGANVGDDSFQSYAKGSNSGRVNFANYGKSFNHGNDTFKEYGRGSIGKTT 365
              +TF+NYR+ +NVGDD F +Y K +N+G  NF NYG+SFN G D F  YG+G      
Sbjct: 310 GPSETFTNYRDESNVGDDKFSNYVKDANAGEANFTNYGQSFNEGTDVFITYGKGG-NDPH 369

Query: 366 VGFKSYSVDRNFTTYAQKGVSFSEYSNFTT---ATGSGIN-----VNRWVEPGKFFRESE 425
           + FK+Y V+  F  Y +   +FS Y N T+   A+ S +N      NRW+EPGKFFRE  
Sbjct: 370 INFKTYGVNNTFKDYVKDTATFSNYHNKTSQDLASLSEVNGGKKVNNRWIEPGKFFREKM 429

Query: 426 LKQGNVMVMPDIRDKMPPRSFLPRPILVNLPFSSSSLNQTKQIFHARDGSTMERVITNAL 485
           LK G +M MPDI+DKMP RSFLPR I   LPFS+S +++ K+IFHA + S + ++I +AL
Sbjct: 430 LKSGTIMPMPDIKDKMPKRSFLPRAIAAKLPFSTSKIDELKKIFHAANDSQVAKMIGDAL 489

Query: 486 AECERAPSRSETKRCVGSAEDMIDFATAVLGRNVVARTTANVDGSTEKVEIGTVKGINGG 545
           +ECERAPS  ETK+CV SAEDMIDFAT+VLGRNVV RTT N +GS   + IG++KGINGG
Sbjct: 490 SECERAPSPGETKQCVNSAEDMIDFATSVLGRNVVVRTTENTNGSKGNIMIGSIKGINGG 549

Query: 546 ELTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILKLKTKEKINRGVAICHMDTSSWGPEH 605
           ++T+SVSCHQ+LYP LLYYCHSVPKVR+YEADIL   +K KIN GVAICH+DTSSWGP H
Sbjct: 550 KVTKSVSCHQTLYPSLLYYCHSVPKVRVYEADILDPNSKAKINHGVAICHVDTSSWGPRH 609

Query: 606 GAFVALGSGPGRIEVCHWIFENDMSWSVAD 626
           GAF+ALGSGPG+IEVCHWIFENDM+W+ AD
Sbjct: 610 GAFIALGSGPGKIEVCHWIFENDMTWATAD 629

BLAST of Spg021747 vs. ExPASy Swiss-Prot
Match: Q40161 (Polygalacturonase-1 non-catalytic subunit beta OS=Solanum lycopersicum OX=4081 GN=GP1 PE=1 SV=1)

HSP 1 Score: 626.7 bits (1615), Expect = 2.8e-178
Identity = 338/629 (53.74%), Postives = 414/629 (65.82%), Query Frame = 0

Query: 8   LLLSHLSLSLFHVSFASADATLNASIQFSPFTAKASLARYWNSHVSNDLPKPDFLFSKAS 67
           LLL   SL  F+V       + N      PFT K  L RYW   +SNDLPKP FL +KAS
Sbjct: 12  LLLLLFSLPSFNVVVGGDGESGN------PFTPKGYLIRYWKKQISNDLPKPWFLLNKAS 71

Query: 68  PLSAVDAAVLAKHAAG-GSLYSRFESFCYLANLFCSFDSETEISVPRSKDADFKLYGNRN 127
           PL+A   A   K  A   +L ++  +FC  ANL C+ D    +    S D  F  Y ++N
Sbjct: 72  PLNAAQYATYTKLVADQNALTTQLHTFCSSANLMCAPDLSPSLE-KHSGDIHFATYSDKN 131

Query: 128 FVNYGAARLG-GVDSFKNYSDGLNSADNSFKRYGGNSSNHREGFTNYAEDGNVATSNFVS 187
           F NYG    G GV++FKNYS+G N   NSF+RYG  S    + F NYA DGNV   +F S
Sbjct: 132 FTNYGTNEPGIGVNTFKNYSEGENIPVNSFRRYGRGSPRDNK-FDNYASDGNVIDQSFNS 191

Query: 188 YAAESKGGSGEFTNYDKLVNVPNLRFTAYDLDSRSHNLSFTSYGNETNSGSQSFTSYGKN 247
           Y+  + GGSG+FTNY    N PNL FT+Y          FT Y  E N+G Q F SYGKN
Sbjct: 192 YSTSTAGGSGKFTNYAANANDPNLHFTSYSDQGTGGVQKFTIYSQEANAGDQYFKSYGKN 251

Query: 248 GNGVPQEFKSYSESSNILTSTFTGYGESGKTANDSFKAYGLSSNNPHTNFKNYGNGGNSQ 307
           GNG   EF SY   +N++ STFT YG++    +  F +YG + N P  +F NYG GGN  
Sbjct: 252 GNGANGEFVSYGNDTNVIGSTFTNYGQTANGGDQKFTSYGFNGNVPENHFTNYGAGGNGP 311

Query: 308 IDTFSNYRNGANVGDDSFQSYAKGSNSGRVNFANYGKSFNHGNDTFKEYGRGSIGKTTVG 367
            +TF++YR+ +NVGDD+F +Y K +N G  NF NYG+SFN G D F  YG+G      + 
Sbjct: 312 SETFNSYRDQSNVGDDTFTTYVKDANGGEANFTNYGQSFNEGTDVFTTYGKGG-NDPHIN 371

Query: 368 FKSYSVDRNFTTYAQKGVSFSEYSNFTTAT---------GSGINVNRWVEPGKFFRESEL 427
           FK+Y V+  F  Y +   +FS Y N T+           G  +N NRWVEPGKFFRE  L
Sbjct: 372 FKTYGVNNTFKDYVKDTATFSNYHNKTSQVLASLMEVNGGKKVN-NRWVEPGKFFREKML 431

Query: 428 KQGNVMVMPDIRDKMPPRSFLPRPILVNLPFSSSSLNQTKQIFHARDGSTMERVITNALA 487
           K G +M MPDI+DKMP RSFLPR I   LPFS+S + + K+IFHA D S +E++I +AL+
Sbjct: 432 KSGTIMPMPDIKDKMPKRSFLPRVIASKLPFSTSKIAELKKIFHAGDESQVEKMIGDALS 491

Query: 488 ECERAPSRSETKRCVGSAEDMIDFATAVLGRNVVARTTANVDGSTEKVEIGTVKGINGGE 547
           ECERAPS  ETKRCV SAEDMIDFAT+VLGRNVV RTT +  GS   + IG+VKGINGG+
Sbjct: 492 ECERAPSAGETKRCVNSAEDMIDFATSVLGRNVVVRTTEDTKGSNGNIMIGSVKGINGGK 551

Query: 548 LTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILKLKTKEKINRGVAICHMDTSSWGPEHG 607
           +T+SVSCHQ+LYPYLLYYCHSVPKVR+YEADIL   +K KIN GVAICH+DTSSWGP HG
Sbjct: 552 VTKSVSCHQTLYPYLLYYCHSVPKVRVYEADILDPNSKVKINHGVAICHVDTSSWGPSHG 611

Query: 608 AFVALGSGPGRIEVCHWIFENDMSWSVAD 626
           AFVALGSGPG+IEVCHWIFENDM+W++AD
Sbjct: 612 AFVALGSGPGKIEVCHWIFENDMTWAIAD 630

BLAST of Spg021747 vs. ExPASy TrEMBL
Match: A0A6J1JM21 (polygalacturonase 1 beta-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111485620 PE=4 SV=1)

HSP 1 Score: 1095.9 bits (2833), Expect = 0.0e+00
Identity = 555/626 (88.66%), Postives = 588/626 (93.93%), Query Frame = 0

Query: 1   MVHIVFFLLLSHLSLSLFHVSFASAD-ATLNASIQFSPFTAKASLARYWNSHVSNDLPKP 60
           MVHIVFFLLLS LSLSL HVSF SAD AT NASI F+PFTAKASL RYWN+HVSN LPKP
Sbjct: 6   MVHIVFFLLLSQLSLSLLHVSFVSADQATFNASIHFNPFTAKASLVRYWNTHVSNQLPKP 65

Query: 61  DFLFSKASPLSAVDAAVLAKHAAGGSLYSRFESFCYLANLFCSFDSETEISVPRSKDADF 120
           DFLF KASPLSAVDAAV AKHAA GSL SR E+FCY ANLFCSFDS+ E SVPR KDADF
Sbjct: 66  DFLFVKASPLSAVDAAVFAKHAALGSLTSRLETFCYSANLFCSFDSQ-ETSVPRFKDADF 125

Query: 121 KLYGNRNFVNYGAARLGGVDSFKNYSDGLNSADNSFKRYGGNSSNHREGFTNYAEDGNVA 180
           KLYGN+NF NYGAAR+GG DSFKNYSDGLN+AD+SFKRYGGNSSNH+EGFTNYAEDGNVA
Sbjct: 126 KLYGNKNFNNYGAARIGGADSFKNYSDGLNTADHSFKRYGGNSSNHKEGFTNYAEDGNVA 185

Query: 181 TSNFVSYAAESKGGSGEFTNYDKLVNVPNLRFTAYDLDSRSHNLSFTSYGNETNSGSQSF 240
            +NF+SYAAESKGGSGEF NYDK+VNVPNLRFTAYDLDSR+H LSFTSYGNETNSGSQSF
Sbjct: 186 NNNFISYAAESKGGSGEFVNYDKIVNVPNLRFTAYDLDSRAHKLSFTSYGNETNSGSQSF 245

Query: 241 TSYGKNGNGVPQEFKSYSESSNILTSTFTGYGESGKTANDSFKAYGLSSNNPHTNFKNYG 300
           TSYGKNGNGVPQEF SYS+ SNILTSTFTGYGESGK+ANDSFKAYGLSSNNPH+NFKNYG
Sbjct: 246 TSYGKNGNGVPQEFSSYSDGSNILTSTFTGYGESGKSANDSFKAYGLSSNNPHSNFKNYG 305

Query: 301 NGGNSQIDTFSNYRNGANVGDDSFQSYAKGSNSGRVNFANYGKSFNHGNDTFKEYGRGSI 360
            GG SQIDTFSNYRNGANVGDDSFQSYAK SNSGRVNFA+YG+SFNHGND+FKEY +GSI
Sbjct: 306 KGGKSQIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFASYGQSFNHGNDSFKEYAKGSI 365

Query: 361 GKTTVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGINVNRWVEPGKFFRESELKQG 420
           G+TTVGF+SYSVDRNFTTYAQKGVSFSEYS    ATGSGINVNRWVEPGKFFRESELKQG
Sbjct: 366 GQTTVGFRSYSVDRNFTTYAQKGVSFSEYS----ATGSGINVNRWVEPGKFFRESELKQG 425

Query: 421 NVMVMPDIRDKMPPRSFLPRPILVNLPFSSSSLNQTKQIFHARDGSTMERVITNALAECE 480
           NVMVMPDIRDKMPPRSFLPR ILVN+PFSSS +++TKQIFHAR+GSTMERVIT+ALAECE
Sbjct: 426 NVMVMPDIRDKMPPRSFLPRAILVNIPFSSSKISETKQIFHARNGSTMERVITSALAECE 485

Query: 481 RAPSRSETKRCVGSAEDMIDFATAVLGRNVVARTTANVDGSTEKVEIGTVKGINGGELTR 540
           RAPS  ETKRCVGSAEDMIDFATAVLGRNVVARTTA+V+GSTEKVEIG VKGINGGELTR
Sbjct: 486 RAPSMGETKRCVGSAEDMIDFATAVLGRNVVARTTASVNGSTEKVEIGEVKGINGGELTR 545

Query: 541 SVSCHQSLYPYLLYYCHSVPKVRIYEADILKLKTKEKINRGVAICHMDTSSWGPEHGAFV 600
           SVSCHQSLYPYLLYYCHSVPKVRIYEADIL+LKT EKINRGVAICHMDTSSWGPEHGAFV
Sbjct: 546 SVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTNEKINRGVAICHMDTSSWGPEHGAFV 605

Query: 601 ALGSGPGRIEVCHWIFENDMSWSVAD 626
           ALGSGPG+IEVCHWIFEND+SWSVAD
Sbjct: 606 ALGSGPGQIEVCHWIFENDVSWSVAD 626

BLAST of Spg021747 vs. ExPASy TrEMBL
Match: A0A6J1E242 (polygalacturonase 1 beta-like protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111430079 PE=4 SV=1)

HSP 1 Score: 1095.5 bits (2832), Expect = 0.0e+00
Identity = 554/626 (88.50%), Postives = 587/626 (93.77%), Query Frame = 0

Query: 1   MVHIVFFLLLSHLSLSLFHVSFASAD-ATLNASIQFSPFTAKASLARYWNSHVSNDLPKP 60
           MVHIVFFLLLS LSLSL HVSF SAD AT NASIQF+PFTAKASL RYWN+HVSN LPKP
Sbjct: 6   MVHIVFFLLLSQLSLSLLHVSFVSADQATFNASIQFNPFTAKASLVRYWNTHVSNQLPKP 65

Query: 61  DFLFSKASPLSAVDAAVLAKHAAGGSLYSRFESFCYLANLFCSFDSETEISVPRSKDADF 120
           DFLF+KASPLSAVDAAV AKHAA GSL SR E+FCY ANLFCSFDS+ E SVPR KDADF
Sbjct: 66  DFLFAKASPLSAVDAAVFAKHAALGSLTSRLETFCYSANLFCSFDSQ-ETSVPRFKDADF 125

Query: 121 KLYGNRNFVNYGAARLGGVDSFKNYSDGLNSADNSFKRYGGNSSNHREGFTNYAEDGNVA 180
           KLYGN+NF NYGAAR+GG DSFKNYSDGLN+AD+SFKRYGGNSSNH+EGFTNYAEDGNVA
Sbjct: 126 KLYGNKNFNNYGAARIGGADSFKNYSDGLNTADHSFKRYGGNSSNHKEGFTNYAEDGNVA 185

Query: 181 TSNFVSYAAESKGGSGEFTNYDKLVNVPNLRFTAYDLDSRSHNLSFTSYGNETNSGSQSF 240
            +NF+SYAAESKGGSGEF NYDK+VNVPNLRFTAYDLDSR+H LSFTSYGNETNSGSQSF
Sbjct: 186 NNNFISYAAESKGGSGEFVNYDKIVNVPNLRFTAYDLDSRAHKLSFTSYGNETNSGSQSF 245

Query: 241 TSYGKNGNGVPQEFKSYSESSNILTSTFTGYGESGKTANDSFKAYGLSSNNPHTNFKNYG 300
           TSYGKNGNGVPQEF SYS+ SNILTSTFTGYGESGK+ANDSFKAYGLS NNPH+NFKNYG
Sbjct: 246 TSYGKNGNGVPQEFSSYSDGSNILTSTFTGYGESGKSANDSFKAYGLSGNNPHSNFKNYG 305

Query: 301 NGGNSQIDTFSNYRNGANVGDDSFQSYAKGSNSGRVNFANYGKSFNHGNDTFKEYGRGSI 360
            GG SQIDTFSNYRNGANVGDDSFQSYAK SNSGRVNFA+YG+SFNHGND+FKEYG+GSI
Sbjct: 306 KGGKSQIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFASYGQSFNHGNDSFKEYGKGSI 365

Query: 361 GKTTVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGINVNRWVEPGKFFRESELKQG 420
           G+TTVGF+SYSVDRNFTTYAQKGVSFSEYS    ATGSGINVNRWVEPGKFFRESELKQG
Sbjct: 366 GQTTVGFRSYSVDRNFTTYAQKGVSFSEYS----ATGSGINVNRWVEPGKFFRESELKQG 425

Query: 421 NVMVMPDIRDKMPPRSFLPRPILVNLPFSSSSLNQTKQIFHARDGSTMERVITNALAECE 480
           NVMVMPDIRDKMPPRSFLPR ILVN+PFSSS +++TKQIFHAR+GSTME VIT+ALAECE
Sbjct: 426 NVMVMPDIRDKMPPRSFLPRAILVNIPFSSSKISETKQIFHARNGSTMEHVITSALAECE 485

Query: 481 RAPSRSETKRCVGSAEDMIDFATAVLGRNVVARTTANVDGSTEKVEIGTVKGINGGELTR 540
           RAPS  ETKRCVGSAEDMIDFATAVLGRNVV RTTA+V+GS EKVEIG VKGINGGELTR
Sbjct: 486 RAPSMGETKRCVGSAEDMIDFATAVLGRNVVVRTTASVNGSKEKVEIGEVKGINGGELTR 545

Query: 541 SVSCHQSLYPYLLYYCHSVPKVRIYEADILKLKTKEKINRGVAICHMDTSSWGPEHGAFV 600
           SVSCHQSLYPYLLYYCHSVPKVRIYEADIL+LKT EKINRGVAICHMDTSSWGPEHGAFV
Sbjct: 546 SVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTNEKINRGVAICHMDTSSWGPEHGAFV 605

Query: 601 ALGSGPGRIEVCHWIFENDMSWSVAD 626
           ALGSGPG+IEVCHWIFENDMSWSVAD
Sbjct: 606 ALGSGPGQIEVCHWIFENDMSWSVAD 626

BLAST of Spg021747 vs. ExPASy TrEMBL
Match: A0A1S3BVD2 (polygalacturonase 1 beta-like protein 3 OS=Cucumis melo OX=3656 GN=LOC103493915 PE=4 SV=1)

HSP 1 Score: 1041.6 bits (2692), Expect = 1.3e-300
Identity = 528/648 (81.48%), Postives = 568/648 (87.65%), Query Frame = 0

Query: 1   MVHIV----FFLLLSHLSLSLFHVS-------------------FASADATLNASIQFSP 60
           MVHI     FF  +S LSLS FH++                   F S    ++++   +P
Sbjct: 1   MVHIFSSSSFFFFISQLSLSFFHLTTNKQISFKVLFLFLLPFFFFLSFFPQISSAASSNP 60

Query: 61  FTAKASLARYWNSHVSNDLPKPDFLFSKASPLSAVDAAVLAKHAAGGSLYSRFESFCYLA 120
           FT KASL RYW +HVS DLP PDF  SKASPLS+VDAAVLA HAAG SL SR ESFCY A
Sbjct: 61  FTPKASLVRYWKTHVSTDLPTPDFFLSKASPLSSVDAAVLATHAAGASLSSRLESFCYSA 120

Query: 121 NLFCSFDSETEISVPRSKDADFKLYGNRNFVNYGAARLGGVDSFKNYSDGLNSADNSFKR 180
           NLFCSFDS++EISVPR KDADFKLYGN+NF NYG  RLGG DSFKNYSDGLN+A++SFKR
Sbjct: 121 NLFCSFDSQSEISVPRFKDADFKLYGNKNFANYGGNRLGGTDSFKNYSDGLNTAEHSFKR 180

Query: 181 YGGNSSNHREGFTNYAEDGNVATSNFVSYAAESKGGSGEFTNYDKLVNVPNLRFTAYDLD 240
           YGGNSSNH+EGFT+YAEDGNVA SNFVSY AESK GSG FTNYDKLVNVPNLRFTAYDL+
Sbjct: 181 YGGNSSNHKEGFTSYAEDGNVANSNFVSYGAESKSGSGGFTNYDKLVNVPNLRFTAYDLN 240

Query: 241 SRSHNLSFTSYGNETNSGSQSFTSYGKNGNGVPQEFKSYSESSNILTSTFTGYGESGKTA 300
           SRSH L+FTSYGNETNSGSQSFTSYGKNGNGVPQ+F SYS+ SNILTSTFTGYGESGKTA
Sbjct: 241 SRSHKLTFTSYGNETNSGSQSFTSYGKNGNGVPQDFSSYSDGSNILTSTFTGYGESGKTA 300

Query: 301 NDSFKAYGLSSNNPHTNFKNYGNGGNSQIDTFSNYRNGANVGDDSFQSYAKGSNSGRVNF 360
           NDSFK YG+S NNPHTNFKNYG GGNS+IDTFSNYRNGANVGDDSFQSYAK SNSGRVNF
Sbjct: 301 NDSFKEYGVSGNNPHTNFKNYGKGGNSEIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNF 360

Query: 361 ANYGKSFNHGNDTFKEYGRGSIGKTTVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGS 420
           ANYGKSFNHGND+F EYG+GSIGKTTVGFKSYS+DRNFTTYA+ GVSFSEYSNFTTATGS
Sbjct: 361 ANYGKSFNHGNDSFTEYGKGSIGKTTVGFKSYSIDRNFTTYAKNGVSFSEYSNFTTATGS 420

Query: 421 GINVNRWVEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRPILVNLPFSSSSLNQTKQ 480
           GI+VN  VEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLPR ILVNLPFSSS +++ K+
Sbjct: 421 GIHVNSHVEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRAILVNLPFSSSRISEAKE 480

Query: 481 IFHARDGSTMERVITNALAECERAPSRSETKRCVGSAEDMIDFATAVLGRNVVARTTANV 540
           +FHARDGSTME VITNALAECERAPSR ETKRCVGSAEDMIDFAT VLGR VV RTTANV
Sbjct: 481 MFHARDGSTMESVITNALAECERAPSRGETKRCVGSAEDMIDFATTVLGRRVVVRTTANV 540

Query: 541 DGSTEKVEIGTVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILKLKTKEKI 600
           +GS EKVEIG VKGINGG LTRSVSCHQSLYPYLLYYCHSVPKVRIYEADIL+++TKEKI
Sbjct: 541 NGSKEKVEIGRVKGINGGNLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRVETKEKI 600

Query: 601 NRGVAICHMDTSSWGPEHGAFVALGSGPGRIEVCHWIFENDMSWSVAD 626
           NRGVAICH+DTSSWGPEHGAFVALGS PG+IEVCHWIFENDMSWSVAD
Sbjct: 601 NRGVAICHIDTSSWGPEHGAFVALGSSPGQIEVCHWIFENDMSWSVAD 648

BLAST of Spg021747 vs. ExPASy TrEMBL
Match: A0A5D3BIQ0 (Polygalacturonase 1 beta-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G001790 PE=4 SV=1)

HSP 1 Score: 1037.3 bits (2681), Expect = 2.5e-299
Identity = 513/597 (85.93%), Postives = 550/597 (92.13%), Query Frame = 0

Query: 29  LNASIQFSPFTAKASLARYWNSHVSNDLPKPDFLFSKASPLSAVDAAVLAKHAAGGSLYS 88
           ++++   +PFT KASL RYW +HVS DLP PDF  SKASPLS+VDAAVLA HAAG SL S
Sbjct: 2   ISSAASSNPFTPKASLVRYWKTHVSTDLPTPDFFLSKASPLSSVDAAVLATHAAGASLSS 61

Query: 89  RFESFCYLANLFCSFDSETEISVPRSKDADFKLYGNRNFVNYGAARLGGVDSFKNYSDGL 148
           R ESFCY ANLFCSFDS++EISVPR KDADFKLYGN+NF NYG  RLGG DSFKNYSDGL
Sbjct: 62  RLESFCYSANLFCSFDSQSEISVPRFKDADFKLYGNKNFANYGGNRLGGTDSFKNYSDGL 121

Query: 149 NSADNSFKRYGGNSSNHREGFTNYAEDGNVATSNFVSYAAESKGGSGEFTNYDKLVNVPN 208
           N+A++SFKRYGGNSSNH+EGFT+YAEDGNVA SNFVSY AESK GSG FTNYDKLVNVPN
Sbjct: 122 NTAEHSFKRYGGNSSNHKEGFTSYAEDGNVANSNFVSYGAESKSGSGGFTNYDKLVNVPN 181

Query: 209 LRFTAYDLDSRSHNLSFTSYGNETNSGSQSFTSYGKNGNGVPQEFKSYSESSNILTSTFT 268
           LRFTAYDL+SRSH L+FTSYGNETNSGSQSFTSYGKNGNGVPQ+F SYS+ SNILTSTFT
Sbjct: 182 LRFTAYDLNSRSHKLTFTSYGNETNSGSQSFTSYGKNGNGVPQDFSSYSDGSNILTSTFT 241

Query: 269 GYGESGKTANDSFKAYGLSSNNPHTNFKNYGNGGNSQIDTFSNYRNGANVGDDSFQSYAK 328
           GYGESGKTANDSFK YG+S NNPHTNFKNYG GGNS+IDTFSNYRNGANVGDDSFQSYAK
Sbjct: 242 GYGESGKTANDSFKEYGVSGNNPHTNFKNYGKGGNSEIDTFSNYRNGANVGDDSFQSYAK 301

Query: 329 GSNSGRVNFANYGKSFNHGNDTFKEYGRGSIGKTTVGFKSYSVDRNFTTYAQKGVSFSEY 388
            SNSGRVNFANYGKSFNHGND+F EYG+GSIGKTTVGFKSYS+DRNFTTYA+ GVSFSEY
Sbjct: 302 NSNSGRVNFANYGKSFNHGNDSFTEYGKGSIGKTTVGFKSYSIDRNFTTYAKNGVSFSEY 361

Query: 389 SNFTTATGSGINVNRWVEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRPILVNLPFS 448
           SNFTTATGSGI+VN  VEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLPR ILVNLPFS
Sbjct: 362 SNFTTATGSGIHVNSHVEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRAILVNLPFS 421

Query: 449 SSSLNQTKQIFHARDGSTMERVITNALAECERAPSRSETKRCVGSAEDMIDFATAVLGRN 508
           SS +++ K++FHARDGSTME VITNALAECERAPSR ETKRCVGSAEDMIDFAT VLGR 
Sbjct: 422 SSRISEAKEMFHARDGSTMESVITNALAECERAPSRGETKRCVGSAEDMIDFATTVLGRR 481

Query: 509 VVARTTANVDGSTEKVEIGTVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVRIYEADI 568
           VV RTTANV+GS EKVEIG VKGINGG LTRSVSCHQSLYPYLLYYCHSVPKVRIYEADI
Sbjct: 482 VVVRTTANVNGSKEKVEIGRVKGINGGNLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADI 541

Query: 569 LKLKTKEKINRGVAICHMDTSSWGPEHGAFVALGSGPGRIEVCHWIFENDMSWSVAD 626
           L+++TKEKINRGVAICH+DTSSWGPEHGAFVALGS PG+IEVCHWIFENDMSWSVAD
Sbjct: 542 LRVETKEKINRGVAICHIDTSSWGPEHGAFVALGSSPGQIEVCHWIFENDMSWSVAD 598

BLAST of Spg021747 vs. ExPASy TrEMBL
Match: A0A0A0LP63 (Polygalacturonase-1 non-catalytic subunit beta OS=Cucumis sativus OX=3659 GN=Csa_1G005490 PE=4 SV=1)

HSP 1 Score: 1036.2 bits (2678), Expect = 5.7e-299
Identity = 528/645 (81.86%), Postives = 568/645 (88.06%), Query Frame = 0

Query: 1   MVHIVFFLL--LSHLSLSLFH------VSFASADATLNASIQF------------SPFTA 60
           MVHI  FL+  LS LSLS FH      +SF      L   I F            +PFT 
Sbjct: 1   MVHIFSFLIFFLSQLSLSFFHLTTNKQISFKFLFLFLLPFIFFLSFFPQISSASSNPFTP 60

Query: 61  KASLARYWNSHVSNDLPKPDFLFSKASPLSAVDAAVLAKHAAGGSLYSRFESFCYLANLF 120
           KASL RYW +HVS DLP PDF  SKASPLSAVDAA LA HAAG SL SR ESFC+ ANLF
Sbjct: 61  KASLVRYWKTHVSTDLPTPDFFLSKASPLSAVDAAALATHAAGASLSSRLESFCFSANLF 120

Query: 121 CSFDSETEISVPRSKDADFKLYGNRNFVNYGAARLGGVDSFKNYSDGLNSADNSFKRYGG 180
           CSFDS++E SVPRSKDADFKLYGN+NFVNYG +RLGG DSFKNYSDGLN+A++SFKRYGG
Sbjct: 121 CSFDSQSETSVPRSKDADFKLYGNKNFVNYGGSRLGGTDSFKNYSDGLNTAEHSFKRYGG 180

Query: 181 NSSNHREGFTNYAEDGNVATSNFVSYAAESKGGSGEFTNYDKLVNVPNLRFTAYDLDSRS 240
           NSSNH+EGFT+YAEDGNVA SNFVSY A SK GSGEFTNYDKLVNVPNLRFTAYDL+SRS
Sbjct: 181 NSSNHKEGFTSYAEDGNVANSNFVSYGAGSKSGSGEFTNYDKLVNVPNLRFTAYDLNSRS 240

Query: 241 HNLSFTSYGNETNSGSQSFTSYGKNGNGVPQEFKSYSESSNILTSTFTGYGESGKTANDS 300
           H LSFTSYGNETNSGSQSFTSYGKNGNGVPQ+F SYS+ SNILTSTFTGYGESGKTANDS
Sbjct: 241 HKLSFTSYGNETNSGSQSFTSYGKNGNGVPQDFSSYSDGSNILTSTFTGYGESGKTANDS 300

Query: 301 FKAYGLSSNNPHTNFKNYGNGGNSQIDTFSNYRNGANVGDDSFQSYAKGSNSGRVNFANY 360
           FK YG+S NNPHTNFKNYG GGNS+IDTFSNYRNGANVGDDSFQSYAK SNSGRVNFANY
Sbjct: 301 FKEYGISGNNPHTNFKNYGKGGNSEIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFANY 360

Query: 361 GKSFNHGNDTFKEYGRGSIGKTTVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGIN 420
           GKSFNHGND+F EYG+GS+GKTTVGFKSYSVDRNFTTYA+ GVSFS Y+NFTTATGSGI+
Sbjct: 361 GKSFNHGNDSFTEYGKGSMGKTTVGFKSYSVDRNFTTYAKNGVSFSVYNNFTTATGSGIH 420

Query: 421 VNRWVEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRPILVNLPFSSSSLNQTKQIFH 480
            N  VEPGKFFRESELKQGNVMVMPDI+DKMPPRSFLPR ILVNLPFSSS +++ K++FH
Sbjct: 421 ANSHVEPGKFFRESELKQGNVMVMPDIKDKMPPRSFLPRSILVNLPFSSSRISEAKEMFH 480

Query: 481 ARDGSTMERVITNALAECERAPSRSETKRCVGSAEDMIDFATAVLGRNVVARTTANVDGS 540
           ARDGSTME VITNALAECERAPSR ETKRCVGSAEDMIDFAT VLG+ VV RTTANV+GS
Sbjct: 481 ARDGSTMESVITNALAECERAPSRGETKRCVGSAEDMIDFATTVLGQRVVVRTTANVNGS 540

Query: 541 TEKVEIGTVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILKLKTKEKINRG 600
            EK+EIG VKGINGG+LTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILK++TKEKINRG
Sbjct: 541 KEKIEIGRVKGINGGDLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILKMETKEKINRG 600

Query: 601 VAICHMDTSSWGPEHGAFVALGSGPGRIEVCHWIFENDMSWSVAD 626
           VAICH+DTSSWGPEHGAFVALGSGPG+IEVCHWIFENDMSW+VAD
Sbjct: 601 VAICHIDTSSWGPEHGAFVALGSGPGQIEVCHWIFENDMSWNVAD 645

BLAST of Spg021747 vs. TAIR 10
Match: AT1G70370.1 (polygalacturonase 2 )

HSP 1 Score: 703.0 bits (1813), Expect = 2.2e-202
Identity = 366/624 (58.65%), Postives = 446/624 (71.47%), Query Frame = 0

Query: 7   FLLLSHLSLSLFHVSFASADATLNA-SIQFSPFTAKASLARYWNSHVSNDLPKPDFLFSK 66
           FLLL   S  LF+V            S + +PFT KASL RYWN  +    P+ +FL SK
Sbjct: 5   FLLLQSFSFFLFNVVIVGGRTFGGGFSAEENPFTPKASLVRYWNKEIRGQSPRSEFLISK 64

Query: 67  ASPLSAVDAAVLAKHAAGGSLYSRFESFCYLANLFCSFDSETEISVPRSKDADFKLYGNR 126
           ASPL+AVD+A  +K AA  SL +RF  FC  ANLFC  D    +      D  F +Y  +
Sbjct: 65  ASPLNAVDSATFSKLAAANSLPTRFPDFCSAANLFCFPDLGASLE-KHDDDVKFSVYDQK 124

Query: 127 NFVNYGAARLGGVDSFKNYSDGLNSADNSFKRYGGNSSNHREGFTNYAEDGNVATSNFVS 186
           NF NYG AR GG DSFKNYS   N   +SF+RY  N++ H + FT Y E+ NV    F S
Sbjct: 125 NFTNYGNARAGGADSFKNYSKDGNVVTDSFRRYSRNAAGHDDKFTVYGENSNVVEEGFNS 184

Query: 187 YAAESKGGSGEFTNYDKLVNVPNLRFTAYDLDSRSHNLSFTSYGNETNSGS-QSFTSYGK 246
           Y     GG+G+FTNY   VN P  RFTAY       + +F +Y +E N+G+ QSFTSYGK
Sbjct: 185 YGTFGTGGAGDFTNYQNNVNNPTSRFTAYSDGGNGRSQTFKTYTHEANAGNGQSFTSYGK 244

Query: 247 NGNGVPQEFKSYSESSNILTSTFTGYGESGKTANDSFKAYGLSSNNPHTNFKNYGNGGNS 306
           NGNGVP EF SY  SSN++ S F+ YGESG  AND+F +YG   N P  NF NYG  GN+
Sbjct: 245 NGNGVPNEFTSYGVSSNVIGSGFSNYGESGNAANDTFTSYGSDGNVPQNNFNNYGASGNA 304

Query: 307 QIDTFSNYRNGANVGDDSFQSYAKGSNSGRVNFANYGKSFNHGNDTFKEYGRGSIGKTTV 366
            +DTF+NYR+ ANVGDDSF SYAK SNS +VNF NYG+SFN G++TF  YG+G+ G + +
Sbjct: 305 AVDTFANYRDKANVGDDSFSSYAKDSNSEKVNFVNYGQSFNPGSETFTGYGKGAEG-SKL 364

Query: 367 GFKSYSVDRNFTTYAQKGVSFSEYSNFTT---ATGSGINVNRWVEPGKFFRESELKQGNV 426
            FK+Y+ +  F  YA+KGV+F++Y+  TT     G G  VN+W+EPGKFFRES LK+G V
Sbjct: 365 SFKTYTPNSTFKDYAKKGVAFAKYNVSTTTANTVGDGKTVNKWIEPGKFFRESSLKEGTV 424

Query: 427 MVMPDIRDKMPPRSFLPRPILVNLPFSSSSLNQTKQIFHARDGSTMERVITNALAECERA 486
           + MPDI+DKMP RSFLPR I+  LPFS+S L + K+IFHA + STM  +IT+A+ ECER 
Sbjct: 425 IPMPDIKDKMPKRSFLPRSIITKLPFSTSKLGEIKRIFHAVENSTMGGIITDAVTECERP 484

Query: 487 PSRSETKRCVGSAEDMIDFATAVLGRNVVARTTANVDGSTEKVEIGTVKGINGGELTRSV 546
           PS  ETKRCVGSAEDMIDFAT+VLGR+VV RTT NV GS EKV IG V GINGG+LT++V
Sbjct: 485 PSVGETKRCVGSAEDMIDFATSVLGRSVVLRTTENVAGSKEKVVIGKVNGINGGKLTKAV 544

Query: 547 SCHQSLYPYLLYYCHSVPKVRIYEADILKLKTKEKINRGVAICHMDTSSWGPEHGAFVAL 606
           SCHQSLYPYLLYYCHSVPKVR+YEAD+L+L +K+KIN G+AICHMDTSSWGP HGAF+AL
Sbjct: 545 SCHQSLYPYLLYYCHSVPKVRVYEADLLELNSKKKINHGIAICHMDTSSWGPSHGAFLAL 604

Query: 607 GSGPGRIEVCHWIFENDMSWSVAD 626
           GS PGRIEVCHWIFENDM+W++AD
Sbjct: 605 GSKPGRIEVCHWIFENDMNWAIAD 626

BLAST of Spg021747 vs. TAIR 10
Match: AT1G70370.2 (polygalacturonase 2 )

HSP 1 Score: 703.0 bits (1813), Expect = 2.2e-202
Identity = 366/624 (58.65%), Postives = 446/624 (71.47%), Query Frame = 0

Query: 7   FLLLSHLSLSLFHVSFASADATLNA-SIQFSPFTAKASLARYWNSHVSNDLPKPDFLFSK 66
           FLLL   S  LF+V            S + +PFT KASL RYWN  +    P+ +FL SK
Sbjct: 5   FLLLQSFSFFLFNVVIVGGRTFGGGFSAEENPFTPKASLVRYWNKEIRGQSPRSEFLISK 64

Query: 67  ASPLSAVDAAVLAKHAAGGSLYSRFESFCYLANLFCSFDSETEISVPRSKDADFKLYGNR 126
           ASPL+AVD+A  +K AA  SL +RF  FC  ANLFC  D    +      D  F +Y  +
Sbjct: 65  ASPLNAVDSATFSKLAAANSLPTRFPDFCSAANLFCFPDLGASLE-KHDDDVKFSVYDQK 124

Query: 127 NFVNYGAARLGGVDSFKNYSDGLNSADNSFKRYGGNSSNHREGFTNYAEDGNVATSNFVS 186
           NF NYG AR GG DSFKNYS   N   +SF+RY  N++ H + FT Y E+ NV    F S
Sbjct: 125 NFTNYGNARAGGADSFKNYSKDGNVVTDSFRRYSRNAAGHDDKFTVYGENSNVVEEGFNS 184

Query: 187 YAAESKGGSGEFTNYDKLVNVPNLRFTAYDLDSRSHNLSFTSYGNETNSGS-QSFTSYGK 246
           Y     GG+G+FTNY   VN P  RFTAY       + +F +Y +E N+G+ QSFTSYGK
Sbjct: 185 YGTFGTGGAGDFTNYQNNVNNPTSRFTAYSDGGNGRSQTFKTYTHEANAGNGQSFTSYGK 244

Query: 247 NGNGVPQEFKSYSESSNILTSTFTGYGESGKTANDSFKAYGLSSNNPHTNFKNYGNGGNS 306
           NGNGVP EF SY  SSN++ S F+ YGESG  AND+F +YG   N P  NF NYG  GN+
Sbjct: 245 NGNGVPNEFTSYGVSSNVIGSGFSNYGESGNAANDTFTSYGSDGNVPQNNFNNYGASGNA 304

Query: 307 QIDTFSNYRNGANVGDDSFQSYAKGSNSGRVNFANYGKSFNHGNDTFKEYGRGSIGKTTV 366
            +DTF+NYR+ ANVGDDSF SYAK SNS +VNF NYG+SFN G++TF  YG+G+ G + +
Sbjct: 305 AVDTFANYRDKANVGDDSFSSYAKDSNSEKVNFVNYGQSFNPGSETFTGYGKGAEG-SKL 364

Query: 367 GFKSYSVDRNFTTYAQKGVSFSEYSNFTT---ATGSGINVNRWVEPGKFFRESELKQGNV 426
            FK+Y+ +  F  YA+KGV+F++Y+  TT     G G  VN+W+EPGKFFRES LK+G V
Sbjct: 365 SFKTYTPNSTFKDYAKKGVAFAKYNVSTTTANTVGDGKTVNKWIEPGKFFRESSLKEGTV 424

Query: 427 MVMPDIRDKMPPRSFLPRPILVNLPFSSSSLNQTKQIFHARDGSTMERVITNALAECERA 486
           + MPDI+DKMP RSFLPR I+  LPFS+S L + K+IFHA + STM  +IT+A+ ECER 
Sbjct: 425 IPMPDIKDKMPKRSFLPRSIITKLPFSTSKLGEIKRIFHAVENSTMGGIITDAVTECERP 484

Query: 487 PSRSETKRCVGSAEDMIDFATAVLGRNVVARTTANVDGSTEKVEIGTVKGINGGELTRSV 546
           PS  ETKRCVGSAEDMIDFAT+VLGR+VV RTT NV GS EKV IG V GINGG+LT++V
Sbjct: 485 PSVGETKRCVGSAEDMIDFATSVLGRSVVLRTTENVAGSKEKVVIGKVNGINGGKLTKAV 544

Query: 547 SCHQSLYPYLLYYCHSVPKVRIYEADILKLKTKEKINRGVAICHMDTSSWGPEHGAFVAL 606
           SCHQSLYPYLLYYCHSVPKVR+YEAD+L+L +K+KIN G+AICHMDTSSWGP HGAF+AL
Sbjct: 545 SCHQSLYPYLLYYCHSVPKVRVYEADLLELNSKKKINHGIAICHMDTSSWGPSHGAFLAL 604

Query: 607 GSGPGRIEVCHWIFENDMSWSVAD 626
           GS PGRIEVCHWIFENDM+W++AD
Sbjct: 605 GSKPGRIEVCHWIFENDMNWAIAD 626

BLAST of Spg021747 vs. TAIR 10
Match: AT1G23760.1 (BURP domain-containing protein )

HSP 1 Score: 667.5 bits (1721), Expect = 1.0e-191
Identity = 349/623 (56.02%), Postives = 433/623 (69.50%), Query Frame = 0

Query: 6   FFLLLSHLSLSLFHVSFASADATLNA--SIQFSPFTAKASLARYWNSHVSNDLPKPDFLF 65
           F  LL  LS  L+HV  A  +   N   S + +PFT KASL RYWN  + +  P   FL 
Sbjct: 5   FVFLLPFLS-RLYHVVIAGGERLTNGGFSPEINPFTPKASLVRYWNKQIHHQSPGSAFLL 64

Query: 66  SKASPLSAVDAAVLAKHAAGGSLYSRFESFCYLANLFCSFDSETEISVPRSKDADFKLYG 125
           SKASPL+AV +A  AK A+  +L  R   FC  ANLFC  D    +    S D  F +Y 
Sbjct: 65  SKASPLTAVASATFAKLASENALPDRLPDFCSAANLFCFPDLGPTLE-KHSNDVKFSVYD 124

Query: 126 NRNFVNYGAARLGGVDSFKNYSDGLNSADNSFKRYGGNSSNHREGFTNYAEDGNVATSNF 185
            RNF NYG    GG DSFK YS   N+  +SF+RY  +++ H + FT YA++ NV    F
Sbjct: 125 QRNFTNYGTIHPGGADSFKKYSQNGNAVTDSFRRYSRDAAGHEDKFTGYADNSNVVEEKF 184

Query: 186 VSYAAESKGGSGEFTNYDKLVNVPNLRFTAYDLDSRSHNLSFTSYGNETNSG-SQSFTSY 245
            SY   + GGSG+F NY   VN P  RF AY  +      +F +Y +E NSG  QSFTSY
Sbjct: 185 NSYGTSATGGSGDFKNYQSGVNNPTTRFKAYSDEGNGRAQTFKTYTHEANSGPGQSFTSY 244

Query: 246 GKNGNGVPQEFKSYSESSNILTSTFTGYGESGKTANDSFKAYGLSSNNPHTNFKNYGNGG 305
           GKNGNG P EF SY  SSN++ S F+ YGE+G  AND+FK YG   N P  NFK+YG  G
Sbjct: 245 GKNGNGAPNEFSSYGVSSNVIGSDFSNYGENGNAANDTFKGYGGDGNVPQNNFKSYGASG 304

Query: 306 NSQIDTFSNYRNGANVGDDSFQSYAKGSNSGRVNFANYGKSFNHGNDTFKEYGRGSIGKT 365
           N+ +DTF NYR+ ANVGDDSF SYAK SN  +V+F NYGKS N G+++F  YG+G+ G  
Sbjct: 305 NAAVDTFLNYRDNANVGDDSFSSYAKNSNFEKVDFVNYGKSINPGSESFSGYGKGAEG-N 364

Query: 366 TVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGINVNRWVEPGKFFRESELKQGNVM 425
            + FK+Y+ +  F  Y + GV F++Y+   ++ G G  VN+WVEPGKFFRES LK+G ++
Sbjct: 365 KIDFKTYTQNSTFKDYTKTGVEFAKYNR--SSLGGGKTVNKWVEPGKFFRESMLKEGTLI 424

Query: 426 VMPDIRDKMPPRSFLPRPILVNLPFSSSSLNQTKQIFHARDGSTMERVITNALAECERAP 485
            MPDI+DKMP RSFLPR I+  LPFS+S + + K++FHA D STME +IT+A+ ECER P
Sbjct: 425 WMPDIKDKMPKRSFLPRSIVSKLPFSTSKIAEIKRVFHANDNSTMEGIITDAVRECERPP 484

Query: 486 SRSETKRCVGSAEDMIDFATAVLGRNVVARTTANVDGSTEKVEIGTVKGINGGELTRSVS 545
           + SETKRCVGSAEDMIDFAT+VLGR+VV RTT +V GS EKV IG V GINGG +T+SVS
Sbjct: 485 TVSETKRCVGSAEDMIDFATSVLGRSVVLRTTESVAGSKEKVMIGKVNGINGGRVTKSVS 544

Query: 546 CHQSLYPYLLYYCHSVPKVRIYEADILKLKTKEKINRGVAICHMDTSSWGPEHGAFVALG 605
           CHQSLYPYLLYYCHSVPKVR+YE+D+L  K+K KIN G+AICHMDTS+WG  HGAF+ LG
Sbjct: 545 CHQSLYPYLLYYCHSVPKVRVYESDLLDPKSKAKINHGIAICHMDTSAWGANHGAFMLLG 604

Query: 606 SGPGRIEVCHWIFENDMSWSVAD 626
           S PG+IEVCHWIFENDM+W++AD
Sbjct: 605 SRPGQIEVCHWIFENDMNWAIAD 622

BLAST of Spg021747 vs. TAIR 10
Match: AT1G60390.1 (polygalacturonase 1 )

HSP 1 Score: 664.8 bits (1714), Expect = 6.6e-191
Identity = 340/608 (55.92%), Postives = 428/608 (70.39%), Query Frame = 0

Query: 19  HVSFASADATL-NASIQFSPFTAKASLARYWNSHVSNDLPKPDFLFSKASPLSAVDAAVL 78
           +V FA A  T  N +   +PFT KASL RYWN+H++ D PKP F  SKASPL+AVD+   
Sbjct: 20  NVHFAGAKQTAGNITPSENPFTPKASLIRYWNNHINGDSPKPSFFLSKASPLTAVDSTRF 79

Query: 79  AKHAAGGSLYSRFESFCYLANLFCSFDSETEISVPRSKDADFKLYGNRNFVNYGAARLGG 138
           A  A+  +L +    FC  A LFC  +           D +F  Y  +NF NYG+ RL G
Sbjct: 80  ASLASNHALNTHHSDFCSAAKLFCFPELAAHSLEKHGDDVNFAAYSGKNFTNYGSDRLSG 139

Query: 139 VDSFKNYSDGLNSADNSFKRYGGNSSNHREGFTNYAEDGNVATSNFVSYAAESKGGSGEF 198
            DSFKNYS G N A +SF+RY  NS+ H +GFTNYA + NVA  +F +YA  + GGSGEF
Sbjct: 140 ADSFKNYSGGDNIAVDSFRRYSRNSAGHDDGFTNYAGEVNVADQSFTTYATGTTGGSGEF 199

Query: 199 TNYDKLVNVPNLRFTAYDLDSRSHNLSFTSYGNETNSGSQSFTSYGKNGNGVPQEFKSYS 258
           TNY+   N PN RFT+Y   +   + +FT+Y    N+G QSFTSY KNGNG P EF  Y 
Sbjct: 200 TNYNTDANEPNGRFTSYSDKANGRSQTFTTYSENGNTGYQSFTSYSKNGNGAPNEFSGYG 259

Query: 259 ESSNILTSTFTGYGESGKTANDSFKAYGLSSNNPHTNFKNYGNGGNSQIDTFSNYRNGAN 318
             SN++ + FT YGES   ANDSF +YG + N P   FK YG+GGN  +  F NYR+ +N
Sbjct: 260 TGSNVVNTGFTKYGESANGANDSFTSYGENGNVPVNEFKGYGDGGNGAVYGFKNYRDQSN 319

Query: 319 VGDDSFQSYAKGSNSGRVNFANYGKSFNHGNDTFKEYGRGSIGKTTVGFKSYSVDRNFTT 378
           +G DSF SYAK SN+ +VNF NYGKSFN G+D F  YG+ ++G   V FK+Y   ++F  
Sbjct: 320 IGVDSFSSYAKNSNNEKVNFVNYGKSFNLGSDNFTGYGQDNVG-GNVSFKTYGQGQSFKV 379

Query: 379 YAQKGVSFSEYSNFTTATGSGINVNRWVEPGKFFRESELKQGNVMVMPDIRDKMPPRSFL 438
           Y + GV F+ YSN    + +G  VN+WVE GKFFRE+ LK+G +M MPDI+DKMP R+FL
Sbjct: 380 YTKDGVVFARYSN--NVSSNGKTVNKWVEEGKFFREAMLKEGTLMQMPDIKDKMPKRTFL 439

Query: 439 PRPILVNLPFSSSSLNQTKQIFHARDGSTMERVITNALAECERAPSRSETKRCVGSAEDM 498
           PR I+ NLPFSSS++ +  ++F A + S+M  +I++A++ECER  S  ETKRCVGSAEDM
Sbjct: 440 PRNIVKNLPFSSSTIGEIWRVFGAGENSSMAGIISSAVSECERPASHGETKRCVGSAEDM 499

Query: 499 IDFATAVLGRNVVARTTANVDGSTEKVEIGTVKGINGGELTRSVSCHQSLYPYLLYYCHS 558
           IDFAT+VLGR VV RTT NV GS +KV IG V GINGG++TR+VSCHQSLYPYLLYYCHS
Sbjct: 500 IDFATSVLGRGVVVRTTENVVGSKKKVVIGKVNGINGGDVTRAVSCHQSLYPYLLYYCHS 559

Query: 559 VPKVRIYEADILKLKTKEKINRGVAICHMDTSSWGPEHGAFVALGSGPGRIEVCHWIFEN 618
           VP+VR+YE D+L  K+ EKIN GVAICH+DTS+W P HGAF+ALGSGPG+IEVCHWIFEN
Sbjct: 560 VPRVRVYETDLLDPKSLEKINHGVAICHIDTSAWSPSHGAFLALGSGPGQIEVCHWIFEN 619

Query: 619 DMSWSVAD 626
           DM+W++ D
Sbjct: 620 DMTWNIID 624

BLAST of Spg021747 vs. TAIR 10
Match: AT5G25610.1 (BURP domain-containing protein )

HSP 1 Score: 109.4 bits (272), Expect = 1.1e-23
Identity = 67/218 (30.73%), Postives = 106/218 (48.62%), Query Frame = 0

Query: 410 FFRESELKQGNVM-VMPDIRDKMPPR-SFLPRPILVNLPFSSSSLNQTKQIFHARDGSTM 469
           FF E +L +G  M V  +  D    + +FLPR     +PF S   ++T + F    GS  
Sbjct: 176 FFLEKDLVRGKEMNVRFNAEDGYGGKTAFLPRGEAETVPFGSEKFSETLKRFSVEAGSEE 235

Query: 470 ERVITNALAECERAPSRSETKRCVGSAEDMIDFATAVLGR---NVVARTTANVDGSTEKV 529
             ++   + ECE      E K C  S E M+DF+ + LG+     V+   A  +   +K 
Sbjct: 236 AEMMKKTIEECEARKVSGEEKYCATSLESMVDFSVSKLGKYHVRAVSTEVAKKNAPMQKY 295

Query: 530 EIGTVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILKLKTKEKIN-RGVAI 589
           +I    G+      +SV CH+  YP+ ++YCH      +Y    + L+ +  +  + VA+
Sbjct: 296 KIAAA-GVKKLSDDKSVVCHKQKYPFAVFYCHKAMMTTVY---AVPLEGENGMRAKAVAV 355

Query: 590 CHMDTSSWGPEHGAFVALGSGPGRIEVCHWIFENDMSW 622
           CH +TS+W P H AF  L   PG + VCH++ E  + W
Sbjct: 356 CHKNTSAWNPNHLAFKVLKVKPGTVPVCHFLPETHVVW 389