Spg021632 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg021632
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	metalloendoproteinase 1-like
Location	scaffold2: 17468840 .. 17469766 (+)
RNA-Seq Expression	Spg021632
Synteny	Spg021632

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTAAGTCATTGCTTCTCATCTTCTTAATCTTCCTTCCTCTTTGCTTTTCACTTCCATTGCCCCAAGTTTCTCCATTTGCATTTCTTAATGATCTCCAAGGATGCAAGAAAGGTGATAATCTCAAAGGCATATCTAAGCTCAAGAACTTCTTCCGCTATTACGGTTATTTGAACTATCGAAACAACGCCACCAGCGATCTCAATAGCGACGATGATTCCTTTGACGATCCTCTTGAGTCTGCCATCAAAACTTACCAACAATACTTTCATCTCAACCCTACTGGATCCTTGAATGTCGAGACGATATCCCAACTCGCAACACCTCGGTGCGGTGTTCCAGACATTGTCAATGGAACCACAGGCCGAATGCTGTCGGAACACGAAGACGACAATCACGACCATCACCACCACCACCTTCCTCACGCCGTCTCTCACTACGCTTTCTTCCCAGGAAGGCGTAGGTGGCCATCTTCCAAATACCATCTCACTTATGCGTTTCTTCCTGGCACTCGCGCGGACGCCAAGGCGCCAGTGGCACGAGCATTCGCCACGTGGGCTCGAAACACTCATTTTAAGTTTTCTCTAGCCACGAGCTACCGAAGAGCGGACCTGAAGATTGCTTTCTACAGAGGCAACCACGGAGACGGGTATCCGTTCGACGGTCCCGGGGGGACTTTGGCACATGCTTTCGCCCCGACCGATGGGAGGTTTCACTATGATTCAACTGAGAAATGGGCAGTTGGTGCAGTGAGAGGGCGATATGATTTGCAAACAGTGGCCTTGCATGAGATTGGACACCTTCTTGGACTTGGCCACAGCACAGTTAGAAATGCTATAATGTATCCTTATATCAAATCTGGGACTACTAAGGGTTTGAATGCAGATGACATCAAAGGAATCAAGGTTCTATACAACAGACGCTAA

mRNA sequence

ATGGCTAAGTCATTGCTTCTCATCTTCTTAATCTTCCTTCCTCTTTGCTTTTCACTTCCATTGCCCCAAGTTTCTCCATTTGCATTTCTTAATGATCTCCAAGGATGCAAGAAAGGTGATAATCTCAAAGGCATATCTAAGCTCAAGAACTTCTTCCGCTATTACGGTTATTTGAACTATCGAAACAACGCCACCAGCGATCTCAATAGCGACGATGATTCCTTTGACGATCCTCTTGAGTCTGCCATCAAAACTTACCAACAATACTTTCATCTCAACCCTACTGGATCCTTGAATGTCGAGACGATATCCCAACTCGCAACACCTCGGTGCGGTGTTCCAGACATTGTCAATGGAACCACAGGCCGAATGCTGTCGGAACACGAAGACGACAATCACGACCATCACCACCACCACCTTCCTCACGCCGTCTCTCACTACGCTTTCTTCCCAGGAAGGCGTAGGTGGCCATCTTCCAAATACCATCTCACTTATGCGTTTCTTCCTGGCACTCGCGCGGACGCCAAGGCGCCAGTGGCACGAGCATTCGCCACGTGGGCTCGAAACACTCATTTTAAGTTTTCTCTAGCCACGAGCTACCGAAGAGCGGACCTGAAGATTGCTTTCTACAGAGGCAACCACGGAGACGGGTATCCGTTCGACGGTCCCGGGGGGACTTTGGCACATGCTTTCGCCCCGACCGATGGGAGGTTTCACTATGATTCAACTGAGAAATGGGCAGTTGGTGCAGTGAGAGGGCGATATGATTTGCAAACAGTGGCCTTGCATGAGATTGGACACCTTCTTGGACTTGGCCACAGCACAGTTAGAAATGCTATAATGTATCCTTATATCAAATCTGGGACTACTAAGGGTTTGAATGCAGATGACATCAAAGGAATCAAGGTTCTATACAACAGACGCTAA

Coding sequence (CDS)

ATGGCTAAGTCATTGCTTCTCATCTTCTTAATCTTCCTTCCTCTTTGCTTTTCACTTCCATTGCCCCAAGTTTCTCCATTTGCATTTCTTAATGATCTCCAAGGATGCAAGAAAGGTGATAATCTCAAAGGCATATCTAAGCTCAAGAACTTCTTCCGCTATTACGGTTATTTGAACTATCGAAACAACGCCACCAGCGATCTCAATAGCGACGATGATTCCTTTGACGATCCTCTTGAGTCTGCCATCAAAACTTACCAACAATACTTTCATCTCAACCCTACTGGATCCTTGAATGTCGAGACGATATCCCAACTCGCAACACCTCGGTGCGGTGTTCCAGACATTGTCAATGGAACCACAGGCCGAATGCTGTCGGAACACGAAGACGACAATCACGACCATCACCACCACCACCTTCCTCACGCCGTCTCTCACTACGCTTTCTTCCCAGGAAGGCGTAGGTGGCCATCTTCCAAATACCATCTCACTTATGCGTTTCTTCCTGGCACTCGCGCGGACGCCAAGGCGCCAGTGGCACGAGCATTCGCCACGTGGGCTCGAAACACTCATTTTAAGTTTTCTCTAGCCACGAGCTACCGAAGAGCGGACCTGAAGATTGCTTTCTACAGAGGCAACCACGGAGACGGGTATCCGTTCGACGGTCCCGGGGGGACTTTGGCACATGCTTTCGCCCCGACCGATGGGAGGTTTCACTATGATTCAACTGAGAAATGGGCAGTTGGTGCAGTGAGAGGGCGATATGATTTGCAAACAGTGGCCTTGCATGAGATTGGACACCTTCTTGGACTTGGCCACAGCACAGTTAGAAATGCTATAATGTATCCTTATATCAAATCTGGGACTACTAAGGGTTTGAATGCAGATGACATCAAAGGAATCAAGGTTCTATACAACAGACGCTAA

Protein sequence

MAKSLLLIFLIFLPLCFSLPLPQVSPFAFLNDLQGCKKGDNLKGISKLKNFFRYYGYLNYRNNATSDLNSDDDSFDDPLESAIKTYQQYFHLNPTGSLNVETISQLATPRCGVPDIVNGTTGRMLSEHEDDNHDHHHHHLPHAVSHYAFFPGRRRWPSSKYHLTYAFLPGTRADAKAPVARAFATWARNTHFKFSLATSYRRADLKIAFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVRNAIMYPYIKSGTTKGLNADDIKGIKVLYNRR

Homology

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BLAST of Spg021632 vs. NCBI nr
Match: XP_038880397.1 (metalloendoproteinase 1-like [Benincasa hispida])

HSP 1 Score: 556.6 bits (1433), Expect = 1.3e-154
Identity = 273/309 (88.35%), Postives = 286/309 (92.56%), Query Frame = 0


Query: 5   LLLIFLIFLPLCFSLPLPQVSPFAFLNDLQGCKKGDNLKGISKLKNFFRYYGYLNYRNNA 64
           +LL   IFLPLCFSLPL QVSPFAFLNDLQGCKKGD++ GISKLKNFF YYGYLN+R N 
Sbjct: 8   ILLFISIFLPLCFSLPLLQVSPFAFLNDLQGCKKGDHVNGISKLKNFFHYYGYLNHRINV 67

Query: 65  TSDL-NSD-DDSFDDPLESAIKTYQQYFHLNPTGSLNVETISQLATPRCGVPDIVNGTTG 124
           T  L  +D DD+FDD LESAIKTYQQYFHLNPTG L+VETISQLATPRCGVPDI+NGTT 
Sbjct: 68  TGHLIEADFDDTFDDFLESAIKTYQQYFHLNPTGLLDVETISQLATPRCGVPDIINGTTS 127

Query: 125 RMLSEHEDDN---HDHHHHHLPHAVSHYAFFPGRRRWPSSKYHLTYAFLPGTRADAKAPV 184
           RMLSEH++DN   HDHHHHHLPHAVSHYAFFPGRRRWPS+KY LTYAFLPGTRADAKAPV
Sbjct: 128 RMLSEHDNDNDSEHDHHHHHLPHAVSHYAFFPGRRRWPSTKYRLTYAFLPGTRADAKAPV 187

Query: 185 ARAFATWARNTHFKFSLATSYRRADLKIAFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFH 244
           ARAFATWARNTHFKFSLAT+YRRADLKI FY GNHGDGYPFDGPGGTLAHAFAPTDGRFH
Sbjct: 188 ARAFATWARNTHFKFSLATNYRRADLKIGFYGGNHGDGYPFDGPGGTLAHAFAPTDGRFH 247

Query: 245 YDSTEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVRNAIMYPYIKSGTTKGLNADDIK 304
           YDSTEKWAVGAV+GRYDLQTVALHEIGHLLGLGHS VRNAIMYPYIKSGTTKGLNADDIK
Sbjct: 248 YDSTEKWAVGAVKGRYDLQTVALHEIGHLLGLGHSNVRNAIMYPYIKSGTTKGLNADDIK 307

Query: 305 GIKVLYNRR 309
           GIKVLYNRR
Sbjct: 308 GIKVLYNRR 316

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BLAST of Spg021632 vs. NCBI nr
Match: XP_008443669.1 (PREDICTED: metalloendoproteinase 2-MMP-like [Cucumis melo] >KAA0038230.1 metalloendoproteinase 2-MMP-like [Cucumis melo var. makuwa] >TYK30625.1 metalloendoproteinase 2-MMP-like [Cucumis melo var. makuwa])

HSP 1 Score: 549.7 bits (1415), Expect = 1.6e-152
Identity = 265/307 (86.32%), Postives = 282/307 (91.86%), Query Frame = 0


Query: 4   SLLLIFLIFLPLCFSLPLPQVSPFAFLNDLQGCKKGDNLKGISKLKNFFRYYGYLNYRNN 63
           ++LL   +FLPLCFSLPL QVSPFAFLNDLQGCKKGDN+KGISKLKNFFRYYGYLN++ N
Sbjct: 7   AILLFISVFLPLCFSLPLLQVSPFAFLNDLQGCKKGDNVKGISKLKNFFRYYGYLNHQIN 66

Query: 64  ATSDL--NSDDDSFDDPLESAIKTYQQYFHLNPTGSLNVETISQLATPRCGVPDIVNGTT 123
           AT  L     DD+FDD LE AIKTYQQYFHLNPTGSLN ETISQLATPRCG PDI+N +T
Sbjct: 67  ATGHLIDTDADDTFDDRLEFAIKTYQQYFHLNPTGSLNAETISQLATPRCGNPDIINEST 126

Query: 124 GRMLSEHEDDNHDHHHHHLPHAVSHYAFFPGRRRWPSSKYHLTYAFLPGTRADAKAPVAR 183
           GRMLSEH +++  H HHHLPHAVSHYAFFPGRRRWPS+KY LTYAFLPGTRADAKAPV R
Sbjct: 127 GRMLSEHNNNDSSHDHHHLPHAVSHYAFFPGRRRWPSTKYRLTYAFLPGTRADAKAPVTR 186

Query: 184 AFATWARNTHFKFSLATSYRRADLKIAFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYD 243
           AFATWARNTHFKFSL T+YRRADLKI FYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYD
Sbjct: 187 AFATWARNTHFKFSLITNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYD 246

Query: 244 STEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVRNAIMYPYIKSGTTKGLNADDIKGI 303
           STEKWAVGAV+GRYDLQTVALHEIGHLLGLGHSTVRNAIMYPYI+SG+TKGLNADDIKGI
Sbjct: 247 STEKWAVGAVKGRYDLQTVALHEIGHLLGLGHSTVRNAIMYPYIRSGSTKGLNADDIKGI 306

Query: 304 KVLYNRR 309
           KVLYNRR
Sbjct: 307 KVLYNRR 313

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BLAST of Spg021632 vs. NCBI nr
Match: XP_004139164.1 (metalloendoproteinase 3-MMP [Cucumis sativus] >KGN66657.1 hypothetical protein Csa_007261 [Cucumis sativus])

HSP 1 Score: 545.4 bits (1404), Expect = 3.1e-151
Identity = 268/306 (87.58%), Postives = 279/306 (91.18%), Query Frame = 0


Query: 5   LLLIFLIFLPLCFSLPLPQVSPFAFLNDLQGCKKGDNLKGISKLKNFFRYYGYLNYRNNA 64
           LLLI  IFLPLCFSLPL QVSPFAFLNDLQGCKKGDN+KGISKLKNFFRYYGYLN++ NA
Sbjct: 9   LLLIISIFLPLCFSLPLIQVSPFAFLNDLQGCKKGDNVKGISKLKNFFRYYGYLNHQINA 68

Query: 65  TSDLNSDD--DSFDDPLESAIKTYQQYFHLNPTGSLNVETISQLATPRCGVPDIVNGTTG 124
           T  L   D  D FDD LESAIKTYQQYFHLNPTGSLN ET+SQLATPRCG PDI+N TTG
Sbjct: 69  TGHLIDIDANDIFDDRLESAIKTYQQYFHLNPTGSLNAETLSQLATPRCGNPDIINETTG 128

Query: 125 RMLSEHEDDNHDHHHHHLPHAVSHYAFFPGRRRWPSSKYHLTYAFLPGTRADAKAPVARA 184
           RMLSE  D+   H HHHLPHAVSHYAFFPGR RWPS+KY LTYAFLPGTRADAKAPVARA
Sbjct: 129 RMLSEDIDNVSSHDHHHLPHAVSHYAFFPGRLRWPSTKYRLTYAFLPGTRADAKAPVARA 188

Query: 185 FATWARNTHFKFSLATSYRRADLKIAFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDS 244
           FATWARNTHFKF+L T+YRRADLKI FYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDS
Sbjct: 189 FATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDS 248

Query: 245 TEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVRNAIMYPYIKSGTTKGLNADDIKGIK 304
           TEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTV+NAIMYPYIKSG+TKGLN DDIKGIK
Sbjct: 249 TEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTKGLNVDDIKGIK 308

Query: 305 VLYNRR 309
           VLYNRR
Sbjct: 309 VLYNRR 314

[Close]

BLAST of Spg021632 vs. NCBI nr
Match: XP_023516970.1 (metalloendoproteinase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 543.9 bits (1400), Expect = 9.0e-151
Identity = 264/314 (84.08%), Postives = 281/314 (89.49%), Query Frame = 0


Query: 3   KSLLLIFLIFLPLCFSLPLPQ------VSPFAFLNDLQGCKKGDNLKGISKLKNFFRYYG 62
           K L+L F IFLPLC SLPLP+      VSPFAFLNDLQGCKKGDN+KGISKLKNFF YYG
Sbjct: 4   KPLILAFFIFLPLCLSLPLPELPELPRVSPFAFLNDLQGCKKGDNVKGISKLKNFFHYYG 63

Query: 63  YLNYRNNATSDLNSDDDSFDDPLESAIKTYQQYFHLNPTGSLNVETISQLATPRCGVPDI 122
           YLN R N TS +N D+D FDD LE AIKTYQQYFHLNPTG+LN ETISQLATPRCGVPDI
Sbjct: 64  YLNRRTNVTSYMNDDNDVFDDHLEFAIKTYQQYFHLNPTGTLNPETISQLATPRCGVPDI 123

Query: 123 VNGTTGRMLSEHEDDNHDH--HHHHLPHAVSHYAFFPGRRRWPSSKYHLTYAFLPGTRAD 182
           VNGTTGR+L EH+DD+ D   HH+HLPH VSHYAFFPG+RRWPSSKY LTYAFLP TR D
Sbjct: 124 VNGTTGRLLFEHDDDDDDDRVHHYHLPHVVSHYAFFPGKRRWPSSKYRLTYAFLPSTRKD 183

Query: 183 AKAPVARAFATWARNTHFKFSLATSYRRADLKIAFYRGNHGDGYPFDGPGGTLAHAFAPT 242
           AKAPV RAFATWAR THFKFSL T+Y+RA+LKI FY+GNHGDGYPFDGPGGTLAHAFAPT
Sbjct: 184 AKAPVTRAFATWARYTHFKFSLTTNYKRANLKIGFYKGNHGDGYPFDGPGGTLAHAFAPT 243

Query: 243 DGRFHYDSTEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVRNAIMYPYIKSGTTKGLN 302
           DGRFHYDSTEKWAVGAV+GRYDLQTVALHEIGHLLGLGHSTV+NAIMYPYIKSGTTKGLN
Sbjct: 244 DGRFHYDSTEKWAVGAVKGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGTTKGLN 303

Query: 303 ADDIKGIKVLYNRR 309
           ADDIKGIKVLYNRR
Sbjct: 304 ADDIKGIKVLYNRR 317

[Close]

BLAST of Spg021632 vs. NCBI nr
Match: KAG7023772.1 (Metalloendoproteinase 3-MMP, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 540.0 bits (1390), Expect = 1.3e-149
Identity = 263/315 (83.49%), Postives = 279/315 (88.57%), Query Frame = 0


Query: 3   KSLLLIFLIFLPLCFSLP---------LPQVSPFAFLNDLQGCKKGDNLKGISKLKNFFR 62
           K L+L F IFLPLC SLP         LPQVSPFAFLNDLQGCKKGDN+KGISKLKNFF 
Sbjct: 4   KPLILAFFIFLPLCLSLPLPELPEWPELPQVSPFAFLNDLQGCKKGDNVKGISKLKNFFH 63

Query: 63  YYGYLNYRNNATSDLNSDDDSFDDPLESAIKTYQQYFHLNPTGSLNVETISQLATPRCGV 122
           YYGYLN+  N T  LN  DD FDDPLE AIKTYQ+YFHLNPTG+LN ETISQLATPRCGV
Sbjct: 64  YYGYLNHGINVTGYLNDVDDVFDDPLEFAIKTYQRYFHLNPTGTLNPETISQLATPRCGV 123

Query: 123 PDIVNGTTGRMLSEHEDDNHDHHHHHLPHAVSHYAFFPGRRRWPSSKYHLTYAFLPGTRA 182
           PDIVNGTTGR+L EH+DD H  H +HLPH VSHYAFFPG+RRWPSSKY LTYAFLPGTR 
Sbjct: 124 PDIVNGTTGRLLFEHDDDGH-VHRYHLPHVVSHYAFFPGKRRWPSSKYRLTYAFLPGTRK 183

Query: 183 DAKAPVARAFATWARNTHFKFSLATSYRRADLKIAFYRGNHGDGYPFDGPGGTLAHAFAP 242
           DAKAPV RAFATWAR THFKFSL T+Y+RA+LKI FY+GNHGDGYPFDGPGGTLAHAFAP
Sbjct: 184 DAKAPVTRAFATWARYTHFKFSLTTNYKRANLKIGFYKGNHGDGYPFDGPGGTLAHAFAP 243

Query: 243 TDGRFHYDSTEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVRNAIMYPYIKSGTTKGL 302
           TDGRFHYDSTEKWAVGAV+GRYDLQTVALHEIGHLLGLGHSTV+NAIMYPYIKSGTTKGL
Sbjct: 244 TDGRFHYDSTEKWAVGAVKGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGTTKGL 303

Query: 303 NADDIKGIKVLYNRR 309
           NADDIKGIKVLYN+R
Sbjct: 304 NADDIKGIKVLYNKR 317

[Close]

BLAST of Spg021632 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 4.9e-59
Identity = 124/287 (43.21%), Postives = 171/287 (59.58%), Query Frame = 0


Query: 31  NDLQGCKKGDNLKGISKLKNFFRYYGYL--NYRNNATSDLNSDDDSFDDPLESAIKTYQQ 90
           ++  GC  G N+ G+ ++K +F+ +GY+   +  N T       D FDD L++A++ YQ 
Sbjct: 45  SNFTGCHHGQNVDGLYRIKKYFQRFGYIPETFSGNFT-------DDFDDILKAAVELYQT 104

Query: 91  YFHLNPTGSLNVETISQLATPRCGVPDIVNGTTGRMLSEHEDDNHDHHHHHLPHAVSHYA 150
            F+LN TG L+  TI  +  PRCG PD+VNGT+       +    +    HL HAV  Y 
Sbjct: 105 NFNLNVTGELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHL-HAVKRYT 164

Query: 151 FFPGRRRWPSSKYHLTYAFLPGT--RADAKAPVARAFATWARNTHFKFSLATSYRRADLK 210
            FPG  RWP ++  LTYAF P      + K+  +RAF  W+  T   F+L+ S+  +D+ 
Sbjct: 165 LFPGEPRWPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDIT 224

Query: 211 IAFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAVG-------AVRGRYDLQT 270
           I FY G+HGDG PFDG  GTLAHAF+P  G+FH D+ E W V        +V    DL++
Sbjct: 225 IGFYTGDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLES 284

Query: 271 VALHEIGHLLGLGHSTVRNAIMYPYIKSGTTK-GLNADDIKGIKVLY 306
           VA+HEIGHLLGLGHS+V  +IMYP I +G  K  L  DD++GI+ LY
Sbjct: 285 VAVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

[Close]

BLAST of Spg021632 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 228.8 bits (582), Expect = 8.4e-59
Identity = 128/279 (45.88%), Postives = 160/279 (57.35%), Query Frame = 0


Query: 39  GDNLKGISKLKNFFRYYGYLNYRNNATSDLNSDDDSFDDPLESAIKTYQQYFHLNPTGSL 98
           G N KG+S +KN+F + GY+        +    DD+FDD L SAIKTYQ+ ++LN TG  
Sbjct: 48  GQNYKGLSNVKNYFHHLGYI-------PNAPHFDDNFDDTLVSAIKTYQKNYNLNVTGKF 107

Query: 99  NVETISQLATPRCGVPDIV---NGTTGRMLSEHEDDNHDHHHHHLPHAVSHYAFFPGRRR 158
           ++ T+ Q+ TPRCGVPDI+   N TT   +                  +S Y FF    R
Sbjct: 108 DINTLKQIMTPRCGVPDIIINTNKTTSFGM------------------ISDYTFFKDMPR 167

Query: 159 WPSSKYHLTYAFLPGTRAD--AKAPVARAFATWARNTHFKFSLATSYRRADLKIAFYRGN 218
           W +    LTYAF P  R D   K+ +ARAF+ W    +  F   TSY  A++KI F   N
Sbjct: 168 WQAGTTQLTYAFSPEPRLDDTFKSAIARAFSKWTPVVNIAFQETTSYETANIKILFASKN 227

Query: 219 HGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAVGA------VRGRYDLQTVALHEIGH 278
           HGD YPFDGPGG L HAFAPTDGR H+D+ E W          V   +DL++VA+HEIGH
Sbjct: 228 HGDPYPFDGPGGILGHAFAPTDGRCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGH 287

Query: 279 LLGLGHSTVRNAIMYPYIKSGTTK-GLNADDIKGIKVLY 306
           LLGLGHS+   AIMYP I   T K  L  DDI GI+ LY
Sbjct: 288 LLGLGHSSDLRAIMYPSIPPRTRKVNLAQDDIDGIRKLY 301

[Close]

BLAST of Spg021632 vs. ExPASy Swiss-Prot
Match: Q5XF51 (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 1.6e-57
Identity = 125/293 (42.66%), Postives = 167/293 (57.00%), Query Frame = 0


Query: 28  AFLNDLQGCKKGDNLKGISKLKNFFRYYGYLNYRNNATSDLNSDDDSFDDPLESAIKTYQ 87
           +FLN   GC  G    G+  LK +F+++GY+   N +     +  D FDD L++A++ YQ
Sbjct: 45  SFLN-FTGCHAGKKYDGLYMLKQYFQHFGYITETNLS----GNFTDDFDDILKNAVEMYQ 104

Query: 88  QYFHLNPTGSLNVETISQLATPRCGVPDIVNGT----TGRMLSEHEDDNHDHHHHHLPHA 147
           + F LN TG L+  T+  +  PRCG PD+VNGT    +GR   E              HA
Sbjct: 105 RNFQLNVTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRF----HA 164

Query: 148 VSHYAFFPGRRRWPSSKYHLTYAFLP--GTRADAKAPVARAFATWARNTHFKFSLATSYR 207
           V HY+FFPG  RWP ++  LTYAF P      + K+  +RAF  W   T   F+    + 
Sbjct: 165 VKHYSFFPGEPRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFS 224

Query: 208 RADLKIAFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAVG--------AVRG 267
            +D+ I FY G HGDG PFDGP  TLAHAF+P  G FH D  E W V         +V  
Sbjct: 225 TSDISIGFYSGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSE 284

Query: 268 RYDLQTVALHEIGHLLGLGHSTVRNAIMYPYIKSGTTK-GLNADDIKGIKVLY 306
             DL++VA+HEIGHLLGLGHS+V  +IMYP I++G  K  L  DD++G++ LY
Sbjct: 285 AVDLESVAVHEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

[Close]

BLAST of Spg021632 vs. ExPASy Swiss-Prot
Match: Q9ZUJ5 (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 2.7e-57
Identity = 136/331 (41.09%), Postives = 179/331 (54.08%), Query Frame = 0


Query: 3   KSLLLIFLIF------LPLCFSLPLPQVSPFAFLN----------DLQGCKKGDNLKGIS 62
           ++LLL  LIF      +   F   +  + P  FLN           L GC  G+N+ G+S
Sbjct: 2   RTLLLTILIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGENINGLS 61

Query: 63  KLKNFFRYYGYLNYRNNATSDLNSDDDSFDDPLESAIKTYQQYFHLNPTGSLNVETISQL 122
           KLK +FR +GY+    N T       D FDD L+SAI TYQ+ F+L  TG L+  T+ Q+
Sbjct: 62  KLKQYFRRFGYITTTGNCT-------DDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQI 121

Query: 123 ATPRCGVPDIVNGTTGRMLSEHEDDNHDHHHHHLPHAVSHYAFFPGRRRWPSSKYHLTYA 182
             PRCG PD+++G +            + +   +      Y+FFPG+ RWP  K  LTYA
Sbjct: 122 VKPRCGNPDLIDGVS------------EMNGGKILRTTEKYSFFPGKPRWPKRKRDLTYA 181

Query: 183 FLPGTRA--DAKAPVARAFATWARNTHFKFSLATSYRRADLKIAFYRGNHGDGYPFDGPG 242
           F P      + K   +RAF  WA  T   F+ + S  RAD+ I F+ G HGDG PFDG  
Sbjct: 182 FAPQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAM 241

Query: 243 GTLAHAFAPTDGRFHYDSTEKWAV--GAVRGR-------YDLQTVALHEIGHLLGLGHST 302
           GTLAHA +P  G  H D  E W +  G +  R        DL++VA+HEIGHLLGLGHS+
Sbjct: 242 GTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSS 301

Query: 303 VRNAIMYPYIKSGTTK-GLNADDIKGIKVLY 306
           V +AIM+P I  G  K  L  DDI+GI+ LY
Sbjct: 302 VEDAIMFPAISGGDRKVELAKDDIEGIQHLY 313

[Close]

BLAST of Spg021632 vs. ExPASy Swiss-Prot
Match: O23507 (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 1.5e-47
Identity = 113/279 (40.50%), Postives = 151/279 (54.12%), Query Frame = 0


Query: 39  GDNLKGISKLKNFFRYYGYLNYRNNATSDLNSDDDSFDDPLESAIKTYQQYFHLNPTGSL 98
           G ++ G+S+LK +   +GY+N  +   SD+      FD PLESAI  YQ+   L  TG L
Sbjct: 64  GSHVSGVSELKRYLHRFGYVNDGSEIFSDV------FDGPLESAISLYQENLGLPITGRL 123

Query: 99  NVETISQLATPRCGVPDIVNGTTGRMLSEHEDDNHDHHHHHLPHAVSHYAFFPGRRRWPS 158
           +  T++ ++ PRCGV D            H   N+D       H  +HY +F G+ +W  
Sbjct: 124 DTSTVTLMSLPRCGVSD-----------THMTINND-----FLHTTAHYTYFNGKPKW-- 183

Query: 159 SKYHLTYAFLPG------TRADAKAPVARAFATWARNTHFKFSLATSYRRADLKIAFYRG 218
           ++  LTYA          T  D K    RAF+ W+      F     +  ADLKI FY G
Sbjct: 184 NRDTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAG 243

Query: 219 NHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAV-----GAVRGRYDLQTVALHEIGH 278
           +HGDG PFDG  GTLAHAFAP +GR H D+ E W V     G+     DL++VA HEIGH
Sbjct: 244 DHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGH 303

Query: 279 LLGLGHSTVRNAIMYPYIKSGTTK-GLNADDIKGIKVLY 306
           LLGLGHS+  +A+MYP ++  T K  L  DD+ G+  LY
Sbjct: 304 LLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

[Close]

BLAST of Spg021632 vs. ExPASy TrEMBL
Match: A0A5A7T4D6 (Metalloendoproteinase 2-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84664G00410 PE=3 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 7.9e-153
Identity = 265/307 (86.32%), Postives = 282/307 (91.86%), Query Frame = 0


Query: 4   SLLLIFLIFLPLCFSLPLPQVSPFAFLNDLQGCKKGDNLKGISKLKNFFRYYGYLNYRNN 63
           ++LL   +FLPLCFSLPL QVSPFAFLNDLQGCKKGDN+KGISKLKNFFRYYGYLN++ N
Sbjct: 7   AILLFISVFLPLCFSLPLLQVSPFAFLNDLQGCKKGDNVKGISKLKNFFRYYGYLNHQIN 66

Query: 64  ATSDL--NSDDDSFDDPLESAIKTYQQYFHLNPTGSLNVETISQLATPRCGVPDIVNGTT 123
           AT  L     DD+FDD LE AIKTYQQYFHLNPTGSLN ETISQLATPRCG PDI+N +T
Sbjct: 67  ATGHLIDTDADDTFDDRLEFAIKTYQQYFHLNPTGSLNAETISQLATPRCGNPDIINEST 126

Query: 124 GRMLSEHEDDNHDHHHHHLPHAVSHYAFFPGRRRWPSSKYHLTYAFLPGTRADAKAPVAR 183
           GRMLSEH +++  H HHHLPHAVSHYAFFPGRRRWPS+KY LTYAFLPGTRADAKAPV R
Sbjct: 127 GRMLSEHNNNDSSHDHHHLPHAVSHYAFFPGRRRWPSTKYRLTYAFLPGTRADAKAPVTR 186

Query: 184 AFATWARNTHFKFSLATSYRRADLKIAFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYD 243
           AFATWARNTHFKFSL T+YRRADLKI FYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYD
Sbjct: 187 AFATWARNTHFKFSLITNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYD 246

Query: 244 STEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVRNAIMYPYIKSGTTKGLNADDIKGI 303
           STEKWAVGAV+GRYDLQTVALHEIGHLLGLGHSTVRNAIMYPYI+SG+TKGLNADDIKGI
Sbjct: 247 STEKWAVGAVKGRYDLQTVALHEIGHLLGLGHSTVRNAIMYPYIRSGSTKGLNADDIKGI 306

Query: 304 KVLYNRR 309
           KVLYNRR
Sbjct: 307 KVLYNRR 313

[Close]

BLAST of Spg021632 vs. ExPASy TrEMBL
Match: A0A1S3B8J8 (metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103487215 PE=3 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 7.9e-153
Identity = 265/307 (86.32%), Postives = 282/307 (91.86%), Query Frame = 0


Query: 4   SLLLIFLIFLPLCFSLPLPQVSPFAFLNDLQGCKKGDNLKGISKLKNFFRYYGYLNYRNN 63
           ++LL   +FLPLCFSLPL QVSPFAFLNDLQGCKKGDN+KGISKLKNFFRYYGYLN++ N
Sbjct: 7   AILLFISVFLPLCFSLPLLQVSPFAFLNDLQGCKKGDNVKGISKLKNFFRYYGYLNHQIN 66

Query: 64  ATSDL--NSDDDSFDDPLESAIKTYQQYFHLNPTGSLNVETISQLATPRCGVPDIVNGTT 123
           AT  L     DD+FDD LE AIKTYQQYFHLNPTGSLN ETISQLATPRCG PDI+N +T
Sbjct: 67  ATGHLIDTDADDTFDDRLEFAIKTYQQYFHLNPTGSLNAETISQLATPRCGNPDIINEST 126

Query: 124 GRMLSEHEDDNHDHHHHHLPHAVSHYAFFPGRRRWPSSKYHLTYAFLPGTRADAKAPVAR 183
           GRMLSEH +++  H HHHLPHAVSHYAFFPGRRRWPS+KY LTYAFLPGTRADAKAPV R
Sbjct: 127 GRMLSEHNNNDSSHDHHHLPHAVSHYAFFPGRRRWPSTKYRLTYAFLPGTRADAKAPVTR 186

Query: 184 AFATWARNTHFKFSLATSYRRADLKIAFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYD 243
           AFATWARNTHFKFSL T+YRRADLKI FYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYD
Sbjct: 187 AFATWARNTHFKFSLITNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYD 246

Query: 244 STEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVRNAIMYPYIKSGTTKGLNADDIKGI 303
           STEKWAVGAV+GRYDLQTVALHEIGHLLGLGHSTVRNAIMYPYI+SG+TKGLNADDIKGI
Sbjct: 247 STEKWAVGAVKGRYDLQTVALHEIGHLLGLGHSTVRNAIMYPYIRSGSTKGLNADDIKGI 306

Query: 304 KVLYNRR 309
           KVLYNRR
Sbjct: 307 KVLYNRR 313

[Close]

BLAST of Spg021632 vs. ExPASy TrEMBL
Match: A0A0A0M0J6 (ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G654860 PE=3 SV=1)

HSP 1 Score: 545.4 bits (1404), Expect = 1.5e-151
Identity = 268/306 (87.58%), Postives = 279/306 (91.18%), Query Frame = 0


Query: 5   LLLIFLIFLPLCFSLPLPQVSPFAFLNDLQGCKKGDNLKGISKLKNFFRYYGYLNYRNNA 64
           LLLI  IFLPLCFSLPL QVSPFAFLNDLQGCKKGDN+KGISKLKNFFRYYGYLN++ NA
Sbjct: 9   LLLIISIFLPLCFSLPLIQVSPFAFLNDLQGCKKGDNVKGISKLKNFFRYYGYLNHQINA 68

Query: 65  TSDLNSDD--DSFDDPLESAIKTYQQYFHLNPTGSLNVETISQLATPRCGVPDIVNGTTG 124
           T  L   D  D FDD LESAIKTYQQYFHLNPTGSLN ET+SQLATPRCG PDI+N TTG
Sbjct: 69  TGHLIDIDANDIFDDRLESAIKTYQQYFHLNPTGSLNAETLSQLATPRCGNPDIINETTG 128

Query: 125 RMLSEHEDDNHDHHHHHLPHAVSHYAFFPGRRRWPSSKYHLTYAFLPGTRADAKAPVARA 184
           RMLSE  D+   H HHHLPHAVSHYAFFPGR RWPS+KY LTYAFLPGTRADAKAPVARA
Sbjct: 129 RMLSEDIDNVSSHDHHHLPHAVSHYAFFPGRLRWPSTKYRLTYAFLPGTRADAKAPVARA 188

Query: 185 FATWARNTHFKFSLATSYRRADLKIAFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDS 244
           FATWARNTHFKF+L T+YRRADLKI FYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDS
Sbjct: 189 FATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDS 248

Query: 245 TEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVRNAIMYPYIKSGTTKGLNADDIKGIK 304
           TEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTV+NAIMYPYIKSG+TKGLN DDIKGIK
Sbjct: 249 TEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTKGLNVDDIKGIK 308

Query: 305 VLYNRR 309
           VLYNRR
Sbjct: 309 VLYNRR 314

[Close]

BLAST of Spg021632 vs. ExPASy TrEMBL
Match: A0A6J1H9F9 (metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111461758 PE=3 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 1.1e-149
Identity = 263/318 (82.70%), Postives = 280/318 (88.05%), Query Frame = 0


Query: 3   KSLLLIFLIFLPLCFSLP------------LPQVSPFAFLNDLQGCKKGDNLKGISKLKN 62
           K L+L F IFLPLC SLP            LPQVSPFAFLNDLQGCKKGDN+KGISKLKN
Sbjct: 4   KPLILAFFIFLPLCLSLPLPELPELPEWPELPQVSPFAFLNDLQGCKKGDNVKGISKLKN 63

Query: 63  FFRYYGYLNYRNNATSDLNSDDDSFDDPLESAIKTYQQYFHLNPTGSLNVETISQLATPR 122
           FF YYGYLN+  N T  LN  DD FDDPLESAIKTYQ+YFHLNPTG+LN+ETISQLATPR
Sbjct: 64  FFHYYGYLNHGINVTGYLNDVDDVFDDPLESAIKTYQRYFHLNPTGTLNLETISQLATPR 123

Query: 123 CGVPDIVNGTTGRMLSEHEDDNHDHHHHHLPHAVSHYAFFPGRRRWPSSKYHLTYAFLPG 182
           CGVPDIVNGTTGR+L EH+DD H  H +HL H VSHYAFFPG+RRWPSSKY LTYAFLPG
Sbjct: 124 CGVPDIVNGTTGRLLFEHDDDGH-VHRYHLSHVVSHYAFFPGKRRWPSSKYRLTYAFLPG 183

Query: 183 TRADAKAPVARAFATWARNTHFKFSLATSYRRADLKIAFYRGNHGDGYPFDGPGGTLAHA 242
           TR DAKAPV RAFATWAR THFKFSL T+Y+RA+LKI FY+GNHGDGYPFDGPGGTLAHA
Sbjct: 184 TRKDAKAPVTRAFATWARYTHFKFSLTTNYKRANLKIGFYKGNHGDGYPFDGPGGTLAHA 243

Query: 243 FAPTDGRFHYDSTEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVRNAIMYPYIKSGTT 302
           FAPTDGRFHYDSTEKWAVGAV+GRYDLQTVALHEIGHLLGLGHSTV+NAIMYPYIKSGTT
Sbjct: 244 FAPTDGRFHYDSTEKWAVGAVKGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGTT 303

Query: 303 KGLNADDIKGIKVLYNRR 309
           KGLNADDIKGIKVLYN+R
Sbjct: 304 KGLNADDIKGIKVLYNKR 320

[Close]

BLAST of Spg021632 vs. ExPASy TrEMBL
Match: A0A6J1JJJ4 (LOW QUALITY PROTEIN: metalloendoproteinase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111485012 PE=3 SV=1)

HSP 1 Score: 510.8 bits (1314), Expect = 4.1e-141
Identity = 252/319 (79.00%), Postives = 271/319 (84.95%), Query Frame = 0


Query: 3   KSLLLIFLIFLPLCFSLP------------LPQVSPFAFLNDLQGCKKGDNLKGISKLKN 62
           K L+L F IFLPLC SLP            LPQVSPFAFLNDL GCKKGDN+KGISKLKN
Sbjct: 4   KPLVLAFFIFLPLCLSLPLPELPELPERPELPQVSPFAFLNDLHGCKKGDNVKGISKLKN 63

Query: 63  FFRYYGYLNYRNNATSDLNSDDDSFDDPLESAIKTYQQYFHLNPTGSLNVETISQLATPR 122
           FF YYGYLN+  N TS  N  +D+FDD LESAIKTYQQYFHLNPTG+LN ETISQLATPR
Sbjct: 64  FFHYYGYLNHGTNITSYTNDVNDAFDDHLESAIKTYQQYFHLNPTGTLNPETISQLATPR 123

Query: 123 CGVPDIVNGTTGRMLSEHEDDNHDH-HHHHLPHAVSHYAFFPGRRRWPSSKYHLTYAFLP 182
           CGVPDIVN TT  +L + +DD+ D  H +HLPH VSHYAFFPG+RRWPSSKY LTYAFLP
Sbjct: 124 CGVPDIVNETTSXLLFKEDDDDDDRVHLYHLPHVVSHYAFFPGKRRWPSSKYRLTYAFLP 183

Query: 183 GTRADAKAPVARAFATWARNTHFKFSLATSYRRADLKIAFYRGNHGDGYPFDGPGGTLAH 242
            TR DAKA V RAF  WAR THFKFSL T+Y+RA+LKI FY+GNHGDGYPFDGPGGTLAH
Sbjct: 184 STRKDAKALVTRAFTIWARYTHFKFSLTTNYKRANLKIGFYKGNHGDGYPFDGPGGTLAH 243

Query: 243 AFAPTDGRFHYDSTEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVRNAIMYPYIKSGT 302
           AFAPTDGRFHYDSTEKWAVGAV+GRYDLQTVALHEIGHLLGLGHSTV+NAIMYPYIKSGT
Sbjct: 244 AFAPTDGRFHYDSTEKWAVGAVKGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGT 303

Query: 303 TKGLNADDIKGIKVLYNRR 309
           TKGLNADDIKGIKVLY +R
Sbjct: 304 TKGLNADDIKGIKVLYKKR 322

[Close]

BLAST of Spg021632 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 229.6 bits (584), Expect = 3.5e-60
Identity = 124/287 (43.21%), Postives = 171/287 (59.58%), Query Frame = 0


Query: 31  NDLQGCKKGDNLKGISKLKNFFRYYGYL--NYRNNATSDLNSDDDSFDDPLESAIKTYQQ 90
           ++  GC  G N+ G+ ++K +F+ +GY+   +  N T       D FDD L++A++ YQ 
Sbjct: 45  SNFTGCHHGQNVDGLYRIKKYFQRFGYIPETFSGNFT-------DDFDDILKAAVELYQT 104

Query: 91  YFHLNPTGSLNVETISQLATPRCGVPDIVNGTTGRMLSEHEDDNHDHHHHHLPHAVSHYA 150
            F+LN TG L+  TI  +  PRCG PD+VNGT+       +    +    HL HAV  Y 
Sbjct: 105 NFNLNVTGELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHL-HAVKRYT 164

Query: 151 FFPGRRRWPSSKYHLTYAFLPGT--RADAKAPVARAFATWARNTHFKFSLATSYRRADLK 210
            FPG  RWP ++  LTYAF P      + K+  +RAF  W+  T   F+L+ S+  +D+ 
Sbjct: 165 LFPGEPRWPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDIT 224

Query: 211 IAFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAVG-------AVRGRYDLQT 270
           I FY G+HGDG PFDG  GTLAHAF+P  G+FH D+ E W V        +V    DL++
Sbjct: 225 IGFYTGDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLES 284

Query: 271 VALHEIGHLLGLGHSTVRNAIMYPYIKSGTTK-GLNADDIKGIKVLY 306
           VA+HEIGHLLGLGHS+V  +IMYP I +G  K  L  DD++GI+ LY
Sbjct: 285 VAVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

[Close]

BLAST of Spg021632 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 224.6 bits (571), Expect = 1.1e-58
Identity = 125/293 (42.66%), Postives = 167/293 (57.00%), Query Frame = 0


Query: 28  AFLNDLQGCKKGDNLKGISKLKNFFRYYGYLNYRNNATSDLNSDDDSFDDPLESAIKTYQ 87
           +FLN   GC  G    G+  LK +F+++GY+   N +     +  D FDD L++A++ YQ
Sbjct: 45  SFLN-FTGCHAGKKYDGLYMLKQYFQHFGYITETNLS----GNFTDDFDDILKNAVEMYQ 104

Query: 88  QYFHLNPTGSLNVETISQLATPRCGVPDIVNGT----TGRMLSEHEDDNHDHHHHHLPHA 147
           + F LN TG L+  T+  +  PRCG PD+VNGT    +GR   E              HA
Sbjct: 105 RNFQLNVTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRF----HA 164

Query: 148 VSHYAFFPGRRRWPSSKYHLTYAFLP--GTRADAKAPVARAFATWARNTHFKFSLATSYR 207
           V HY+FFPG  RWP ++  LTYAF P      + K+  +RAF  W   T   F+    + 
Sbjct: 165 VKHYSFFPGEPRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFS 224

Query: 208 RADLKIAFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAVG--------AVRG 267
            +D+ I FY G HGDG PFDGP  TLAHAF+P  G FH D  E W V         +V  
Sbjct: 225 TSDISIGFYSGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSE 284

Query: 268 RYDLQTVALHEIGHLLGLGHSTVRNAIMYPYIKSGTTK-GLNADDIKGIKVLY 306
             DL++VA+HEIGHLLGLGHS+V  +IMYP I++G  K  L  DD++G++ LY
Sbjct: 285 AVDLESVAVHEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

[Close]

BLAST of Spg021632 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 223.8 bits (569), Expect = 1.9e-58
Identity = 136/331 (41.09%), Postives = 179/331 (54.08%), Query Frame = 0


Query: 3   KSLLLIFLIF------LPLCFSLPLPQVSPFAFLN----------DLQGCKKGDNLKGIS 62
           ++LLL  LIF      +   F   +  + P  FLN           L GC  G+N+ G+S
Sbjct: 2   RTLLLTILIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGENINGLS 61

Query: 63  KLKNFFRYYGYLNYRNNATSDLNSDDDSFDDPLESAIKTYQQYFHLNPTGSLNVETISQL 122
           KLK +FR +GY+    N T       D FDD L+SAI TYQ+ F+L  TG L+  T+ Q+
Sbjct: 62  KLKQYFRRFGYITTTGNCT-------DDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQI 121

Query: 123 ATPRCGVPDIVNGTTGRMLSEHEDDNHDHHHHHLPHAVSHYAFFPGRRRWPSSKYHLTYA 182
             PRCG PD+++G +            + +   +      Y+FFPG+ RWP  K  LTYA
Sbjct: 122 VKPRCGNPDLIDGVS------------EMNGGKILRTTEKYSFFPGKPRWPKRKRDLTYA 181

Query: 183 FLPGTRA--DAKAPVARAFATWARNTHFKFSLATSYRRADLKIAFYRGNHGDGYPFDGPG 242
           F P      + K   +RAF  WA  T   F+ + S  RAD+ I F+ G HGDG PFDG  
Sbjct: 182 FAPQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAM 241

Query: 243 GTLAHAFAPTDGRFHYDSTEKWAV--GAVRGR-------YDLQTVALHEIGHLLGLGHST 302
           GTLAHA +P  G  H D  E W +  G +  R        DL++VA+HEIGHLLGLGHS+
Sbjct: 242 GTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSS 301

Query: 303 VRNAIMYPYIKSGTTK-GLNADDIKGIKVLY 306
           V +AIM+P I  G  K  L  DDI+GI+ LY
Sbjct: 302 VEDAIMFPAISGGDRKVELAKDDIEGIQHLY 313

[Close]

BLAST of Spg021632 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 191.4 bits (485), Expect = 1.1e-48
Identity = 113/279 (40.50%), Postives = 151/279 (54.12%), Query Frame = 0


Query: 39  GDNLKGISKLKNFFRYYGYLNYRNNATSDLNSDDDSFDDPLESAIKTYQQYFHLNPTGSL 98
           G ++ G+S+LK +   +GY+N  +   SD+      FD PLESAI  YQ+   L  TG L
Sbjct: 64  GSHVSGVSELKRYLHRFGYVNDGSEIFSDV------FDGPLESAISLYQENLGLPITGRL 123

Query: 99  NVETISQLATPRCGVPDIVNGTTGRMLSEHEDDNHDHHHHHLPHAVSHYAFFPGRRRWPS 158
           +  T++ ++ PRCGV D            H   N+D       H  +HY +F G+ +W  
Sbjct: 124 DTSTVTLMSLPRCGVSD-----------THMTINND-----FLHTTAHYTYFNGKPKW-- 183

Query: 159 SKYHLTYAFLPG------TRADAKAPVARAFATWARNTHFKFSLATSYRRADLKIAFYRG 218
           ++  LTYA          T  D K    RAF+ W+      F     +  ADLKI FY G
Sbjct: 184 NRDTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAG 243

Query: 219 NHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAV-----GAVRGRYDLQTVALHEIGH 278
           +HGDG PFDG  GTLAHAFAP +GR H D+ E W V     G+     DL++VA HEIGH
Sbjct: 244 DHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGH 303

Query: 279 LLGLGHSTVRNAIMYPYIKSGTTK-GLNADDIKGIKVLY 306
           LLGLGHS+  +A+MYP ++  T K  L  DD+ G+  LY
Sbjct: 304 LLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

[Close]

BLAST of Spg021632 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 171.4 bits (433), Expect = 1.1e-42
Identity = 108/273 (39.56%), Postives = 137/273 (50.18%), Query Frame = 0


Query: 45  ISKLKNFFRYYGYLNYRNNATSDLNSDDDSFDDPLESAIKTYQQYFHLNPTGSLNVETIS 104
           I ++K   + YGYL        +  SDD SF    E A+  YQ+   L  TG  + +T+S
Sbjct: 50  IPEIKRHLQQYGYL------PQNKESDDVSF----EQALVRYQKNLGLPITGKPDSDTLS 109

Query: 105 QLATPRCGVPDIVNGTTGRMLSEHEDDNHDHHHHHLPHAVSHYAFFPGRRRWPSS-KYHL 164
           Q+  PRCG PD V   T                    H    Y +FPGR RW       L
Sbjct: 110 QILLPRCGFPDDVEPKTAPF-----------------HTGKKYVYFPGRPRWTRDVPLKL 169

Query: 165 TYAFLPGTRADAKAPV------ARAFATWARNTHFKFSLATSYRRADLKIAFYRGNHGDG 224
           TYAF         AP        RAF  WA      F     Y  AD+KI F+ G+HGDG
Sbjct: 170 TYAFSQENLTPYLAPTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDG 229

Query: 225 YPFDGPGGTLAHAFAPTDGRFHYDSTEKWAVGAVRGR----YDLQTVALHEIGHLLGLGH 284
            PFDG  G LAH F+P +GR H D  E WAV     +     DL++VA+HEIGH+LGLGH
Sbjct: 230 EPFDGVLGVLAHTFSPENGRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGH 289

Query: 285 STVRNAIMYPYIKSGTTK-GLNADDIKGIKVLY 306
           S+V++A MYP +K  + K  LN DD+ G++ LY
Sbjct: 290 SSVKDAAMYPTLKPRSKKVNLNMDDVVGVQSLY 295

The following BLAST results are available for this feature:

BLAST of Spg021632 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038880397.1	1.3e-154	88.35	metalloendoproteinase 1-like [Benincasa hispida]	[more]
XP_008443669.1	1.6e-152	86.32	PREDICTED: metalloendoproteinase 2-MMP-like [Cucumis melo] >KAA0038230.1 metallo...	[more]
XP_004139164.1	3.1e-151	87.58	metalloendoproteinase 3-MMP [Cucumis sativus] >KGN66657.1 hypothetical protein C...	[more]
XP_023516970.1	9.0e-151	84.08	metalloendoproteinase 1-like [Cucurbita pepo subsp. pepo]	[more]
KAG7023772.1	1.3e-149	83.49	Metalloendoproteinase 3-MMP, partial [Cucurbita argyrosperma subsp. argyrosperma...	[more]

BLAST of Spg021632 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04529	4.9e-59	43.21	Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1	[more]
P29136	8.4e-59	45.88	Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2	[more]
Q5XF51	1.6e-57	42.66	Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1	[more]
Q9ZUJ5	2.7e-57	41.09	Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1	[more]
O23507	1.5e-47	40.50	Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1	[more]

BLAST of Spg021632 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7T4D6	7.9e-153	86.32	Metalloendoproteinase 2-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3B8J8	7.9e-153	86.32	metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103487215 PE=3 SV...	[more]
A0A0A0M0J6	1.5e-151	87.58	ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G654860 PE=3 S...	[more]
A0A6J1H9F9	1.1e-149	82.70	metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111461758 PE=3 ...	[more]
A0A6J1JJJ4	4.1e-141	79.00	LOW QUALITY PROTEIN: metalloendoproteinase 1-like OS=Cucurbita maxima OX=3661 GN...	[more]

BLAST of Spg021632 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G70170.1	3.5e-60	43.21	matrix metalloproteinase	[more]
AT1G24140.1	1.1e-58	42.66	Matrixin family protein	[more]
AT1G59970.1	1.9e-58	41.09	Matrixin family protein	[more]
AT4G16640.1	1.1e-48	40.50	Matrixin family protein	[more]
AT2G45040.1	1.1e-42	39.56	Matrixin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021190	Peptidase M10A	PRINTS	PR00138	MATRIXIN	coord: 203..231 score: 62.95 coord: 260..285 score: 58.3 coord: 293..306 score: 51.57 coord: 179..194 score: 33.23 coord: 106..119 score: 50.7
IPR021190	Peptidase M10A	PIRSF	PIRSF001191	MMP_stromelysin	coord: 15..308 e-value: 2.0E-62 score: 209.4
IPR006026	Peptidase, metallopeptidase	SMART	SM00235	col_5	coord: 152..307 e-value: 2.4E-36 score: 136.7
IPR001818	Peptidase M10, metallopeptidase	PFAM	PF00413	Peptidase_M10	coord: 156..305 e-value: 1.2E-45 score: 155.2
IPR024079	Metallopeptidase, catalytic domain superfamily	GENE3D	3.40.390.10	Collagenase (Catalytic Domain)	coord: 36..308 e-value: 6.1E-76 score: 257.1
IPR002477	Peptidoglycan binding-like	PFAM	PF01471	PG_binding_1	coord: 46..106 e-value: 2.5E-7 score: 30.8
None	No IPR available	PANTHER	PTHR10201:SF269	METALLOENDOPROTEINASE 3-MMP-LIKE	coord: 7..306
None	No IPR available	PANTHER	PTHR10201	MATRIX METALLOPROTEINASE	coord: 7..306
None	No IPR available	SUPERFAMILY	55486	Metalloproteases ("zincins"), catalytic domain	coord: 149..306
IPR021158	Peptidase M10A, cysteine switch, zinc binding site	PROSITE	PS00546	CYSTEINE_SWITCH	coord: 109..116
IPR033739	Peptidase M10A, catalytic domain	CDD	cd04278	ZnMc_MMP	coord: 155..305 e-value: 3.99517E-62 score: 192.419
IPR036365	PGBD-like superfamily	SUPERFAMILY	47090	PGBD-like	coord: 36..116

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg021632.1	Spg021632.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030574	collagen catabolic process
biological_process	GO:0030198	extracellular matrix organization
biological_process	GO:0006508	proteolysis
cellular_component	GO:0031225	anchored component of membrane
cellular_component	GO:0031012	extracellular matrix
molecular_function	GO:0004222	metalloendopeptidase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0008237	metallopeptidase activity