Spg021595 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg021595
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionProtein DETOXIFICATION
Locationscaffold9: 5226043 .. 5231138 (-)
RNA-Seq ExpressionSpg021595
SyntenySpg021595
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGGAAGTTGTTTTGAAGAGAGAAAAAAATGAGAGAAAAATGGAGAGTTTATGAAAAGGAGCTGAAGAAGGTGAGCTTTTTAGCAGCGCCCATCGTCATAGCGTTTGTTCTTCAATACCTTTTGCAGGTAGAGATTGTCATCATCGTTGGCCATCTCGACGACGAGCTTTTGCTTTCTGGAGTCTCCATTGCCACTTCCTTCGCTCGCGCCACTGGCTTTGGCCTCCTCGTAATCCTTGTTTTCCTTTGTTCTAAGACCATGTCATTTTGTTTTTGAGCTTCTGGTGTTTTTCCCGTTGTTTTTTATCCTAACATAATACCTTGCTTTTTGTAACGCTGAATATATATAAATTCATGAATGAACTGAGACCATGTCCGAATGAGAGAAATTCCGAGCATATAAAGTATGGTTAAGATAAACTTAAAAGAATTTATAATAACTAATGTTCACACTTAAATTCAAAGATGAGACACGTGGAATAATTTCGATTTGTACTGATGATCTATGCGTGTGAGTGTACAATCTCTAATACATAATTCTCTGATTCTCTCTTTTGTGTATGAGTTTCAGTCGAAGGCTGAAACACTTGTTCTCGAATTTGAAGTCGTCTCAAGAGCTTTGCAGCTTGTTCTTGAGATTGAAGTTGTCTCAAGGGCTTTGCTACTTGTTCTTGAGATTGAAGTTGTATCAAGGGCTTCGTGAGTTCAATGAGTCTTTTGATCTTCTGGAAGTCTTCAAGAAGCTTTAGTCTTTCAGAATCTTGAAGGTTTGTTCTTCAAAAGGATATAAGTTCTTTTAGATGGTTTGAGGTAGACTTCAATCTTCAAGTCTGCAAGAGACCCTGAGCTTCAATCGGTTTTCCAGTAAGATTTGAAGAGGATCTGAGCTTCGATCTTCAGTTTTGCAACAAGACTTGAAGAGGATATGAGCTTTGATCTTTGGTTCTGCAGTGGGATTTGAAGAGGATCTGAAGTTTGATCTCTGGCTCTGTAGCGGGATTTGAAGGGGATCTGAGCTTTGTTCTTCAGTTCTGCAGCAGGATTTGAAGAGGATCTGAGCTTCAATCTTCGGTTCGGAAGCGAAATTTGAAGAGAATTTGAGCTTCATTCTTCAATTCAGCAGCTAGATTTGAAGAAGATTTGAGCTTCGATCTTCGATTTTGCAGGAGGAGCTTCAATCTTCAGAGTCTTCTGAGAGCTTCCTAATCTTCCTCTTCTTCCTTTCTTTCTGCCCCCCACCAAATGAGATGCAAGACCTCCTATTTATAGAGTTTTCAAAAGGCCTTCGTGAGCTTGGACTTGATTGCATTTGGGCCCATCCACGGGGTTGGGCTTGATTGCATTTGAGCGCATTCATGGGATTGGACTTGAAATTTTGGGCTTCAAGCCCAATTACCTTAATGAATTTAGATTTATAATAAACCTAATTTAATGAACTTAGCACATTTTAATTTCATGAACTTAGAAAATTACTTGAAAATTTTCTATGCCAACAAATATCTAGACCAACATGATGCACCTTCCTTTTCGGTGGCTCAATTATAGGAACTTATTCAAAGTGAAGTGTTCTTGACTAGGGAAGGGTGACCTCTTGAAGATTTTCCTAGGAAGCATCTGGTGAGGAGGACAAAACGTTGTAAAAGGACCTCTTGTTAATTTGTATGGACAATCTTCACTAAAAAATTAAAGACAAGTATGGTGATGTTGAAGAGTCCTGATCCAAATCTTGAACATGGAATGTTACAATTTCATACTTTTGAAGTTTGTTAGCTGGTTTAGAAGAAGTAGAGGATTAGATATTTATTAGACTTGGTATTTATAGGAAGTTCTAAGGTTTTGGATTTGGTGATAGTTTTACGGCGGAGAGAAAAAATGCGAAGAAGATAAGATACTTGCTTGTCTTGTAGTTTTTCTTTTGTGAATAGGTTTACAATGAGAAGTTGAGATGCTTTTATTTTCTCTCTTTCTTCTAGTTTTTCTTTTGTGTTGCCTTGGGATACTTGTATTTGAAAACTTAAACTTGCAATGAAAATTATCGTTTTCTTTTCCCTATCTTCATATATTTAATAATTTATGAAAAATACATATTTGATATATTAGTCACCACCGAATACAGACAAAAAATCTAGTGATGGATAACAAGAACTTTTTGTTGTTTAGTTGATAAAGAAGGATCATTCATCTTTTATTGACATTCTTTATCATTTCTCTCTCACTTGTGGGTTTCAAAATTAGCACAAGTTTAATGAGTTACAACACAATTTTAACTAAAAATGAAATTTACTTTGATATATCATCATGTTAAATTATATCATTGGAACTCAAGAACTTGAGCTAAAAGGTCAATCATTTCTTTATATTCTTTACCTAGGTTGTTGTTTTGTTGCAGTTGGGAATGGCTGGAGCTTTGGAAACTCTATGTGGGCAAGCATATGGGGCAGAACAATATCAAAAGCTTGGAGTTTATACTTATAGTTGCACAATTTCTCTCATTTTGGTTTGTTTTCCAATCTCCATATTGTGGCTCTTCACAGACAAGTTACTAATTTCCATTGGTCAAGACCCTTCCATTTCGTATGTGGCTAGAAAATACTCAGTTTTTCTCATTCCAAACCTCTTTGCCTATGCAATACTTCAGTCTCTTATGCGCTATCTCTTCACTCAAAGCTTGATCATTCCCTTGCTCGTTTGCTCTTTTGTCACCCTCTCTTTGCATATTCCCATTTCTTGGCTTCTTGTATTCCATTTTAACCTCAAGGTTGTAGGAGCTGCTCTGGCTCTTGGCATATCCTATTGGCTCAATGTCATTTTGTTAGGGCTCTACATCTTCTTCTCTCCATCCTGCAAGAAGACTCGTGCTCCTTTCTCAAGAGATGCCATCTTGAGCATCAGCGAGTTCTTTCGGCTCGCCGTCCCCTCCGCCGTGATGACTTGGTAACCGTCGATCGACTTGGATGTTTTGTTTGATTTGATTTGATTCGTTAAAGCGTGTTTTTGTTGTGTGTTTTGTAGCCTTGAGTGGTGGTCATATGAGGTCATTCTTTTGCTTTCTGGACTTTTACCGAATCCTAAGGTGGAGACTTCTGTGCTTTCTATATGGTATATTATTTATGCTTTCTTAATTTTCTCAAGCATTGCGAACTTAAATTTTTCAAAAGATAAGTCCAAGCCTTTAACTCTTTTTTGGTACTTTTTGGACAGTTTCTCAATCACTTATTTGCATTTTTTCATACCTTATGGGTTGGCGGCCACAGTAAGGTAAGTTCTAATCATTTATTCATTTTTGTTGTGGTCAAAGTTATATAATTTTTCTTTTTGTTTTTTTTTATAAATGAGATTTTTTTTTAAAAAAAAAAACATTATCAAATAGTTCCACCTCTTTTGAGTTACAATAGTATGAGTTATAATCTTCGTTTTTTGCATACATGGTTTTAGTAACATTATTGGAAAGGAAAAGTTTGAGGAGGTCACTGTCTACACATTACTATCTACCCTTCGTTGAATCCATAGACATGTAAAAATGTAGATAGAAATGATGTGGTAAGAGATTCATTACATGGATGTACATAAAGATTTTGAATAATTTGAATGATGATATGTATAGCACAAGGGTTTCAAATGAACTAGGAGCTGGAAATCCTGAGGCAGCTAAGGTGGCAGTGAGGGCAGTGGGAATTCTTGGCATCATTGAATCAACAATTGTGAGTGTGACCCTCTTTGGGTGTCACAATATCTTGGGATATGCATTCACAAGTGACAACCAAATTGCCAAACATATTGCTTCTATGTGGCCTTTAATTTGTCTTTCCATTTTCATTGATAGTTTCCTTGCTATTCTTACAGGTATGTATCTCGGTATCTCGATATTCTAACTTTTAAACCACTTATAGATTCCCTAAATTTTTGTTAATCACAATTTGGTTTCTATATTTGAAGTTTAGGTTCAATCGGATTATCCTAGTTTAAAAAGTTTCAATATTGGATTTTATGATTTAGTTTAAAACTTTTTTTTTTTTTTGAAAAAAGTTTAAAACTTTGTTCTTATAATTATAAGTCGATAAGATATTGAATAGGTGACACTTTTCTATTTATGTGACAATAAAGTAATGCATTCAATTTGGTTAACTATATTTAGTTATACGGTATATATTTTAGGGTTTAAGACAATGTATATGTATATGTATGATAATTTAGAGTGTGTGTGGCAATTTAGGGGTTGCAAGAGGCACTGGATGGCAGCGTTTAGGGGCATATGTGAATTTGGGATCATATTATATAGTTGGGATTCCAATGGCAGCTGTATTGGCTTTTGTTGCACATTTAAGAGTAAAAGGACTTTGGATTGGCTTAGTTTCAGGAGCAGTACTTCAAAGCTTTCTTTTTGCTCTCATTACCATTTTCACTAATTGGCATAAACAGGTTATCTTTCTACATTTTTCCCATTTTTTCTATGATTAGTGTCTTTCAACTACTAATTATTTTTGTAACTGATTTTAATTCTTCAATAACCATTTAACAAATATATAAATTTTTAGACATTTACTCTCGGGTTAAAATTAATTAATGTTAAAAAATTTGGAGATGGAAATAAAATACATAATAAAATTTGAGGACTAAAATAGAATTTGTTTGGTAAATTGAGTCAAAAGACTAAAGTAAGATGCATTGTTTTTAGTTGTAGGATTTTAACCCTTAATAGATTTTTCAAAACCATATATGAACCATCATTGTTGTTTGAATGCTTACATGAATTTATATGGATCATGCAGGCCTTGAAAGCAAGGGGAAGGATACTTGAAGGGAATACCTAACATCTCTGCCCAATATATGAGATAGTTGATGTGGATTTTTCCTACCACAAGCGTACGAGTCAAGTTTTAATATAGTGATTTTCGTATTAACGAGTGTCGTCCTCTGGATTAGGATTCTAACAAAATTAATTACTGCAAATACACTTGTCCAATATTATCTAGGGATTTAAACGTAGTGATAATGAAGAAATCAAGACACAAAAGTTAAAATGTATGAAACTCAAATGATGAAAACTCTTAGGCCCCGTTTCATAACCATTTCGTTTTTGGTTTTTGGTTTTTGAAA

mRNA sequence

ATGAGAGAAAAATGGAGAGTTTATGAAAAGGAGCTGAAGAAGGTGAGCTTTTTAGCAGCGCCCATCGTCATAGCGTTTGTTCTTCAATACCTTTTGCAGGTAGAGATTGTCATCATCGTTGGCCATCTCGACGACGAGCTTTTGCTTTCTGGAGTCTCCATTGCCACTTCCTTCGCTCGCGCCACTGGCTTTGGCCTCCTCTTGGGAATGGCTGGAGCTTTGGAAACTCTATGTGGGCAAGCATATGGGGCAGAACAATATCAAAAGCTTGGAGTTTATACTTATAGTTGCACAATTTCTCTCATTTTGGTTTGTTTTCCAATCTCCATATTGTGGCTCTTCACAGACAAGTTACTAATTTCCATTGGTCAAGACCCTTCCATTTCGTATGTGGCTAGAAAATACTCAGTTTTTCTCATTCCAAACCTCTTTGCCTATGCAATACTTCAGTCTCTTATGCGCTATCTCTTCACTCAAAGCTTGATCATTCCCTTGCTCGTTTGCTCTTTTGTCACCCTCTCTTTGCATATTCCCATTTCTTGGCTTCTTGTATTCCATTTTAACCTCAAGGTTGTAGGAGCTGCTCTGGCTCTTGGCATATCCTATTGGCTCAATGTCATTTTGTTAGGGCTCTACATCTTCTTCTCTCCATCCTGCAAGAAGACTCGTGCTCCTTTCTCAAGAGATGCCATCTTGAGCATCAGCGAGTTCTTTCGGCTCGCCGTCCCCTCCGCCGTGATGACTTGCCTTGAGTGGTGGTCATATGAGGTCATTCTTTTGCTTTCTGGACTTTTACCGAATCCTAAGGTGGAGACTTCTGTGCTTTCTATATGTTTCTCAATCACTTATTTGCATTTTTTCATACCTTATGGGTTGGCGGCCACAGTAAGCACAAGGGTTTCAAATGAACTAGGAGCTGGAAATCCTGAGGCAGCTAAGGTGGCAGTGAGGGCAGTGGGAATTCTTGGCATCATTGAATCAACAATTGTGAGTGTGACCCTCTTTGGGTGTCACAATATCTTGGGATATGCATTCACAAGTGACAACCAAATTGCCAAACATATTGCTTCTATGTGGCCTTTAATTTGTCTTTCCATTTTCATTGATAGTTTCCTTGCTATTCTTACAGGGGTTGCAAGAGGCACTGGATGGCAGCGTTTAGGGGCATATGTGAATTTGGGATCATATTATATAGTTGGGATTCCAATGGCAGCTGTATTGGCTTTTGTTGCACATTTAAGAGTAAAAGGACTTTGGATTGGCTTAGTTTCAGGAGCAGTACTTCAAAGCTTTCTTTTTGCTCTCATTACCATTTTCACTAATTGGCATAAACAGGCCTTGAAAGCAAGGGGAAGGATACTTGAAGGGAATACCTAA

Coding sequence (CDS)

ATGAGAGAAAAATGGAGAGTTTATGAAAAGGAGCTGAAGAAGGTGAGCTTTTTAGCAGCGCCCATCGTCATAGCGTTTGTTCTTCAATACCTTTTGCAGGTAGAGATTGTCATCATCGTTGGCCATCTCGACGACGAGCTTTTGCTTTCTGGAGTCTCCATTGCCACTTCCTTCGCTCGCGCCACTGGCTTTGGCCTCCTCTTGGGAATGGCTGGAGCTTTGGAAACTCTATGTGGGCAAGCATATGGGGCAGAACAATATCAAAAGCTTGGAGTTTATACTTATAGTTGCACAATTTCTCTCATTTTGGTTTGTTTTCCAATCTCCATATTGTGGCTCTTCACAGACAAGTTACTAATTTCCATTGGTCAAGACCCTTCCATTTCGTATGTGGCTAGAAAATACTCAGTTTTTCTCATTCCAAACCTCTTTGCCTATGCAATACTTCAGTCTCTTATGCGCTATCTCTTCACTCAAAGCTTGATCATTCCCTTGCTCGTTTGCTCTTTTGTCACCCTCTCTTTGCATATTCCCATTTCTTGGCTTCTTGTATTCCATTTTAACCTCAAGGTTGTAGGAGCTGCTCTGGCTCTTGGCATATCCTATTGGCTCAATGTCATTTTGTTAGGGCTCTACATCTTCTTCTCTCCATCCTGCAAGAAGACTCGTGCTCCTTTCTCAAGAGATGCCATCTTGAGCATCAGCGAGTTCTTTCGGCTCGCCGTCCCCTCCGCCGTGATGACTTGCCTTGAGTGGTGGTCATATGAGGTCATTCTTTTGCTTTCTGGACTTTTACCGAATCCTAAGGTGGAGACTTCTGTGCTTTCTATATGTTTCTCAATCACTTATTTGCATTTTTTCATACCTTATGGGTTGGCGGCCACAGTAAGCACAAGGGTTTCAAATGAACTAGGAGCTGGAAATCCTGAGGCAGCTAAGGTGGCAGTGAGGGCAGTGGGAATTCTTGGCATCATTGAATCAACAATTGTGAGTGTGACCCTCTTTGGGTGTCACAATATCTTGGGATATGCATTCACAAGTGACAACCAAATTGCCAAACATATTGCTTCTATGTGGCCTTTAATTTGTCTTTCCATTTTCATTGATAGTTTCCTTGCTATTCTTACAGGGGTTGCAAGAGGCACTGGATGGCAGCGTTTAGGGGCATATGTGAATTTGGGATCATATTATATAGTTGGGATTCCAATGGCAGCTGTATTGGCTTTTGTTGCACATTTAAGAGTAAAAGGACTTTGGATTGGCTTAGTTTCAGGAGCAGTACTTCAAAGCTTTCTTTTTGCTCTCATTACCATTTTCACTAATTGGCATAAACAGGCCTTGAAAGCAAGGGGAAGGATACTTGAAGGGAATACCTAA

Protein sequence

MREKWRVYEKELKKVSFLAAPIVIAFVLQYLLQVEIVIIVGHLDDELLLSGVSIATSFARATGFGLLLGMAGALETLCGQAYGAEQYQKLGVYTYSCTISLILVCFPISILWLFTDKLLISIGQDPSISYVARKYSVFLIPNLFAYAILQSLMRYLFTQSLIIPLLVCSFVTLSLHIPISWLLVFHFNLKVVGAALALGISYWLNVILLGLYIFFSPSCKKTRAPFSRDAILSISEFFRLAVPSAVMTCLEWWSYEVILLLSGLLPNPKVETSVLSICFSITYLHFFIPYGLAATVSTRVSNELGAGNPEAAKVAVRAVGILGIIESTIVSVTLFGCHNILGYAFTSDNQIAKHIASMWPLICLSIFIDSFLAILTGVARGTGWQRLGAYVNLGSYYIVGIPMAAVLAFVAHLRVKGLWIGLVSGAVLQSFLFALITIFTNWHKQALKARGRILEGNT
Homology
BLAST of Spg021595 vs. NCBI nr
Match: XP_023535495.1 (protein DETOXIFICATION 8-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 761.5 bits (1965), Expect = 4.1e-216
Identity = 387/458 (84.50%), Postives = 414/458 (90.39%), Query Frame = 0

Query: 1   MREKWRVYEKELKKVSFLAAPIVIAFVLQYLLQVEIVIIVGHLDDELLLSGVSIATSFAR 60
           MREKWRVY+KE+KKVSFLAAPI+I FVLQYLLQV  +IIVGHL +ELLLSG+SIA+SF R
Sbjct: 1   MREKWRVYQKEVKKVSFLAAPIIIVFVLQYLLQVVTIIIVGHLGNELLLSGISIASSFVR 60

Query: 61  ATGFGLLLGMAGALETLCGQAYGAEQYQKLGVYTYSCTISLILVCFPISILWLFTDKLLI 120
            TGF LLLGMAGALETLCGQAYGA+QYQK+GVYTYSC I L+L CFPISILW FTDKLLI
Sbjct: 61  VTGFALLLGMAGALETLCGQAYGAQQYQKIGVYTYSCMICLLLACFPISILWFFTDKLLI 120

Query: 121 SIGQDPSISYVARKYSVFLIPNLFAYAILQSLMRYLFTQSLIIPLLVCSFVTLSLHIPIS 180
           SIGQDPSIS +ARKYS+FLIPNLFA+AILQSLMRYL TQSLI+PLL CS  TLS HIPI 
Sbjct: 121 SIGQDPSISSIARKYSLFLIPNLFAHAILQSLMRYLLTQSLILPLLFCSLATLSFHIPIC 180

Query: 181 WLLVFHFNLKVVGAALALGISYWLNVILLGLYIFFSPSCKKTRAPFSRDAILSISEFFRL 240
           WLLVFHFNLKVVGAALALG+SYWLNVILLGLYI FSPSC KTRAPFS +AI SI +FFRL
Sbjct: 181 WLLVFHFNLKVVGAALALGLSYWLNVILLGLYIVFSPSCNKTRAPFSMEAISSIGKFFRL 240

Query: 241 AVPSAVMTCLEWWSYEVILLLSGLLPNPKVETSVLSICFSITYLHFFIPYGLAATVSTRV 300
           A+PSAVM C EWWSYE+ILLLSGLLPNPKVE SVLSICFS+TYLH+FIPYGL AT STRV
Sbjct: 241 AIPSAVMVCFEWWSYEIILLLSGLLPNPKVEASVLSICFSVTYLHYFIPYGLGATASTRV 300

Query: 301 SNELGAGNPEAAKVAVRAVGILGIIESTIVSVTLFGCHNILGYAFTSDNQIAKHIASMWP 360
           SNELGAGNPEAAKVAV+ VG+LGIIES  VS  LFGCHN+LGYAFTSD QIA HIAS WP
Sbjct: 301 SNELGAGNPEAAKVAVKVVGVLGIIESVTVSAALFGCHNMLGYAFTSDTQIANHIASFWP 360

Query: 361 LICLSIFIDSFLAILTGVARGTGWQRLGAYVNLGSYYIVGIPMAAVLAFVAHLRVKGLWI 420
           LICLSI IDSFL IL+GVARG GWQR+GAYVNLGSYYIVGIPMAAVLAFV HLRVKGLWI
Sbjct: 361 LICLSILIDSFLGILSGVARGCGWQRVGAYVNLGSYYIVGIPMAAVLAFVVHLRVKGLWI 420

Query: 421 GLVSGAVLQSFLFALITIFTNWHKQALKARGRILEGNT 459
           GLVSG  LQSFLFALITIFTNWHKQALKAR R+LE NT
Sbjct: 421 GLVSGVTLQSFLFALITIFTNWHKQALKARERVLEENT 458

BLAST of Spg021595 vs. NCBI nr
Match: XP_022973951.1 (protein DETOXIFICATION 8-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 758.8 bits (1958), Expect = 2.6e-215
Identity = 386/458 (84.28%), Postives = 413/458 (90.17%), Query Frame = 0

Query: 1   MREKWRVYEKELKKVSFLAAPIVIAFVLQYLLQVEIVIIVGHLDDELLLSGVSIATSFAR 60
           MREKWRVY+KE+KKVSFLAAPI+  FVLQYLLQV  VIIVGHL DELLLSG+SIA+SF R
Sbjct: 27  MREKWRVYQKEVKKVSFLAAPIITVFVLQYLLQVVTVIIVGHLGDELLLSGISIASSFVR 86

Query: 61  ATGFGLLLGMAGALETLCGQAYGAEQYQKLGVYTYSCTISLILVCFPISILWLFTDKLLI 120
            TGF LLLGMAGALETLCGQAYGA+QYQK+GVYTYSC I L+L CFPISILW FTDKLLI
Sbjct: 87  VTGFALLLGMAGALETLCGQAYGAQQYQKIGVYTYSCMICLLLACFPISILWFFTDKLLI 146

Query: 121 SIGQDPSISYVARKYSVFLIPNLFAYAILQSLMRYLFTQSLIIPLLVCSFVTLSLHIPIS 180
           SIGQDPSIS +ARKYS+FLIPNLFA+AILQSLMRYL TQSLI+PLL CS  TLS HIPI 
Sbjct: 147 SIGQDPSISSIARKYSIFLIPNLFAHAILQSLMRYLLTQSLILPLLFCSLATLSFHIPIC 206

Query: 181 WLLVFHFNLKVVGAALALGISYWLNVILLGLYIFFSPSCKKTRAPFSRDAILSISEFFRL 240
           WLLVFHFNLKVVGAALALG+SYWLNVILLGLYI FSPSC KTRAPFS +AI SI +FFRL
Sbjct: 207 WLLVFHFNLKVVGAALALGMSYWLNVILLGLYIVFSPSCNKTRAPFSMEAISSIGKFFRL 266

Query: 241 AVPSAVMTCLEWWSYEVILLLSGLLPNPKVETSVLSICFSITYLHFFIPYGLAATVSTRV 300
           A+PSAVM C EWWSYE+ILLLSGLLPNPKVE S+LSICFS+TYLH+FIPYGL AT STRV
Sbjct: 267 AIPSAVMVCFEWWSYEIILLLSGLLPNPKVEASMLSICFSMTYLHYFIPYGLGATASTRV 326

Query: 301 SNELGAGNPEAAKVAVRAVGILGIIESTIVSVTLFGCHNILGYAFTSDNQIAKHIASMWP 360
           SNELGAGNPEAAKVAV+ VG+LGIIES  +S  LFGCHN+LGYAFTSD QIA HIAS WP
Sbjct: 327 SNELGAGNPEAAKVAVKVVGVLGIIESVTMSAALFGCHNMLGYAFTSDTQIANHIASFWP 386

Query: 361 LICLSIFIDSFLAILTGVARGTGWQRLGAYVNLGSYYIVGIPMAAVLAFVAHLRVKGLWI 420
           LICLSI IDSFL IL+GVARG GWQR+GAYVNLGSYYIVGIPMAAVLAFV HLRVKGLWI
Sbjct: 387 LICLSILIDSFLGILSGVARGCGWQRVGAYVNLGSYYIVGIPMAAVLAFVVHLRVKGLWI 446

Query: 421 GLVSGAVLQSFLFALITIFTNWHKQALKARGRILEGNT 459
           GLVSGA LQSFLFALIT FTNWHKQALKAR R+LE NT
Sbjct: 447 GLVSGATLQSFLFALITTFTNWHKQALKARERVLEENT 484

BLAST of Spg021595 vs. NCBI nr
Match: KAG6592362.1 (Protein DETOXIFICATION 14, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 756.9 bits (1953), Expect = 1.0e-214
Identity = 384/458 (83.84%), Postives = 414/458 (90.39%), Query Frame = 0

Query: 1   MREKWRVYEKELKKVSFLAAPIVIAFVLQYLLQVEIVIIVGHLDDELLLSGVSIATSFAR 60
           MREKWRVY+KE+KKVSFLAAPI+I FVLQYLLQV  +IIVGHL +ELLLSG+SIA+SF R
Sbjct: 1   MREKWRVYQKEVKKVSFLAAPIIIVFVLQYLLQVVTIIIVGHLGNELLLSGISIASSFVR 60

Query: 61  ATGFGLLLGMAGALETLCGQAYGAEQYQKLGVYTYSCTISLILVCFPISILWLFTDKLLI 120
            TGF LLLGMAGALETLCGQAYGA+QYQK+GVYTYSC I L+L CFPISI+W FTDKLLI
Sbjct: 61  VTGFALLLGMAGALETLCGQAYGAQQYQKIGVYTYSCMICLLLACFPISIVWFFTDKLLI 120

Query: 121 SIGQDPSISYVARKYSVFLIPNLFAYAILQSLMRYLFTQSLIIPLLVCSFVTLSLHIPIS 180
           SIGQDPSIS +ARKYS+FLIPNLFA+AILQSLMRYL TQSLI+PLL CS  TLS HIPI 
Sbjct: 121 SIGQDPSISSIARKYSLFLIPNLFAHAILQSLMRYLLTQSLILPLLFCSLATLSFHIPIC 180

Query: 181 WLLVFHFNLKVVGAALALGISYWLNVILLGLYIFFSPSCKKTRAPFSRDAILSISEFFRL 240
           WLLVFHFNLKVVGAALALG+SYWLNVILLGLYI FSPSC KT+APFS +AI SI +FFRL
Sbjct: 181 WLLVFHFNLKVVGAALALGMSYWLNVILLGLYIVFSPSCNKTQAPFSMEAISSIGKFFRL 240

Query: 241 AVPSAVMTCLEWWSYEVILLLSGLLPNPKVETSVLSICFSITYLHFFIPYGLAATVSTRV 300
           A+PSAVM C EWWSYE+ILLLSGLLPNPKVE S+LSICFS+TYLH+FIPYGL AT STRV
Sbjct: 241 AIPSAVMVCFEWWSYEIILLLSGLLPNPKVEASMLSICFSVTYLHYFIPYGLGATASTRV 300

Query: 301 SNELGAGNPEAAKVAVRAVGILGIIESTIVSVTLFGCHNILGYAFTSDNQIAKHIASMWP 360
           SNELGAGNPEAAKVAV+ VG+LGIIES  VS  LFGCHN+LGYAFTSD QIA HIAS WP
Sbjct: 301 SNELGAGNPEAAKVAVKVVGVLGIIESVTVSAALFGCHNMLGYAFTSDTQIANHIASFWP 360

Query: 361 LICLSIFIDSFLAILTGVARGTGWQRLGAYVNLGSYYIVGIPMAAVLAFVAHLRVKGLWI 420
           LICLSI IDSFL IL+GVARG GWQR+GAYVNLGSYYIVGIPMAAVLAFV HLRVKGLWI
Sbjct: 361 LICLSILIDSFLGILSGVARGCGWQRVGAYVNLGSYYIVGIPMAAVLAFVVHLRVKGLWI 420

Query: 421 GLVSGAVLQSFLFALITIFTNWHKQALKARGRILEGNT 459
           GLVSGA LQSFLFALIT FTNWHKQALKAR R+LE NT
Sbjct: 421 GLVSGATLQSFLFALITTFTNWHKQALKARERVLEENT 458

BLAST of Spg021595 vs. NCBI nr
Match: XP_022925514.1 (protein DETOXIFICATION 8-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 756.9 bits (1953), Expect = 1.0e-214
Identity = 385/458 (84.06%), Postives = 413/458 (90.17%), Query Frame = 0

Query: 1   MREKWRVYEKELKKVSFLAAPIVIAFVLQYLLQVEIVIIVGHLDDELLLSGVSIATSFAR 60
           MREKWRVY+KE+KKVSFLAAPI+I FVLQYLLQV  +IIVGHL DELLLSG+SIA+SF R
Sbjct: 27  MREKWRVYQKEVKKVSFLAAPIIIVFVLQYLLQVVTIIIVGHLGDELLLSGISIASSFVR 86

Query: 61  ATGFGLLLGMAGALETLCGQAYGAEQYQKLGVYTYSCTISLILVCFPISILWLFTDKLLI 120
            TGF LLLGMAGALETLCGQAYGA+QYQK+GVYTYSC I L+L CFPISI+W FTDKLLI
Sbjct: 87  VTGFALLLGMAGALETLCGQAYGAQQYQKIGVYTYSCMICLLLACFPISIVWFFTDKLLI 146

Query: 121 SIGQDPSISYVARKYSVFLIPNLFAYAILQSLMRYLFTQSLIIPLLVCSFVTLSLHIPIS 180
           SIGQDPSIS +ARKYS+FLIPNLFA+AILQSLMRYL TQSLI+PLL CS  TLS HIPI 
Sbjct: 147 SIGQDPSISSIARKYSLFLIPNLFAHAILQSLMRYLLTQSLILPLLFCSLATLSFHIPIC 206

Query: 181 WLLVFHFNLKVVGAALALGISYWLNVILLGLYIFFSPSCKKTRAPFSRDAILSISEFFRL 240
           WLLVFHFNLKVVGAALALG+SYWLNVILLGLYI FSPSC KT+A FS +AI SI +FFRL
Sbjct: 207 WLLVFHFNLKVVGAALALGMSYWLNVILLGLYIVFSPSCNKTQATFSMEAISSIGKFFRL 266

Query: 241 AVPSAVMTCLEWWSYEVILLLSGLLPNPKVETSVLSICFSITYLHFFIPYGLAATVSTRV 300
           A+PSAVM C EWWSYE+ILLLSGLLPNPKVE SVLSICFS+TYLH+FIPYGL AT STRV
Sbjct: 267 AIPSAVMVCFEWWSYEIILLLSGLLPNPKVEASVLSICFSVTYLHYFIPYGLGATASTRV 326

Query: 301 SNELGAGNPEAAKVAVRAVGILGIIESTIVSVTLFGCHNILGYAFTSDNQIAKHIASMWP 360
           SNELGAGNPEAAKVAV+ VG+LGIIES  VS  LFGCHN+LGYAFTSD QIA HIAS WP
Sbjct: 327 SNELGAGNPEAAKVAVKVVGVLGIIESVTVSAALFGCHNMLGYAFTSDTQIANHIASFWP 386

Query: 361 LICLSIFIDSFLAILTGVARGTGWQRLGAYVNLGSYYIVGIPMAAVLAFVAHLRVKGLWI 420
           LICLSI IDSFL IL+GVARG GWQR+GAYVNLGSYYIVGIPMAAVLAFV HLRVKGLWI
Sbjct: 387 LICLSILIDSFLGILSGVARGCGWQRVGAYVNLGSYYIVGIPMAAVLAFVVHLRVKGLWI 446

Query: 421 GLVSGAVLQSFLFALITIFTNWHKQALKARGRILEGNT 459
           GLVSGA LQSFLFALIT FTNWHKQALKAR R+LE NT
Sbjct: 447 GLVSGATLQSFLFALITTFTNWHKQALKARERVLEENT 484

BLAST of Spg021595 vs. NCBI nr
Match: XP_022141868.1 (protein DETOXIFICATION 1-like isoform X2 [Momordica charantia])

HSP 1 Score: 753.8 bits (1945), Expect = 8.5e-214
Identity = 393/458 (85.81%), Postives = 410/458 (89.52%), Query Frame = 0

Query: 1   MREKWRVYEKELKKVSFLAAPIVIAFVLQYLLQVEIVIIVGHLDDELLLSGVSIATSFAR 60
           MREKWRVY  ELKKVS +AAPI  A VLQYLLQV  V+IVGHL DELLLSGVSIATSF R
Sbjct: 36  MREKWRVYPTELKKVSLIAAPITTALVLQYLLQVVTVVIVGHLGDELLLSGVSIATSFVR 95

Query: 61  ATGFGLLLGMAGALETLCGQAYGAEQYQKLGVYTYSCTISLILVCFPISILWLFTDKLLI 120
            TGF LLLGMAGALETLCGQAYGAEQYQKLGVYTYSC ISLILVCFPIS+LW FTDKLLI
Sbjct: 96  VTGFSLLLGMAGALETLCGQAYGAEQYQKLGVYTYSCMISLILVCFPISVLWFFTDKLLI 155

Query: 121 SIGQDPSISYVARKYSVFLIPNLFAYAILQSLMRYLFTQSLIIPLLVCSFVTLSLHIPIS 180
           SIGQDPSIS VARKYSVFLIPNLFA AILQSL+RY  TQSLI+PLL  SF TL LHIPI 
Sbjct: 156 SIGQDPSISSVARKYSVFLIPNLFACAILQSLLRYFLTQSLILPLLFSSFATLCLHIPIC 215

Query: 181 WLLVFHFNLKVVGAALALGISYWLNVILLGLYIFFSPSCKKTRAPFSRDAILSISEFFRL 240
           WL VFHF L+VVGAALALGISYWLNVILL  Y+FFSPSC KTRAP SR+AI SI +FFRL
Sbjct: 216 WLFVFHFKLRVVGAALALGISYWLNVILLASYVFFSPSCNKTRAPLSREAISSIGQFFRL 275

Query: 241 AVPSAVMTCLEWWSYEVILLLSGLLPNPKVETSVLSICFSITYLHFFIPYGLAATVSTRV 300
           AVPSAVM CLEWWSYEVILLLSGLLPNPKVE SVLSICFSITYLH+FIPYGL ATVSTRV
Sbjct: 276 AVPSAVMVCLEWWSYEVILLLSGLLPNPKVEASVLSICFSITYLHYFIPYGLGATVSTRV 335

Query: 301 SNELGAGNPEAAKVAVRAVGILGIIESTIVSVTLFGCHNILGYAFTSDNQIAKHIASMWP 360
           SNELGAGNPEAAKVAV+ VG LGIIES  VSV LFGC NILGYAFT+D QIA HIASMWP
Sbjct: 336 SNELGAGNPEAAKVAVKVVGALGIIESITVSVILFGCRNILGYAFTNDTQIADHIASMWP 395

Query: 361 LICLSIFIDSFLAILTGVARGTGWQRLGAYVNLGSYYIVGIPMAAVLAFVAHLRVKGLWI 420
           LICLSI ID+FL IL+GVARGTGWQ+LGAYVNLGSYYIVGIPMAAVLAFVAHLRVKGLWI
Sbjct: 396 LICLSILIDTFLGILSGVARGTGWQQLGAYVNLGSYYIVGIPMAAVLAFVAHLRVKGLWI 455

Query: 421 GLVSGAVLQSFLFALITIFTNWHKQALKARGRILEGNT 459
           GLVSGA +QS LFALITIFTNWHKQAL AR R+LEGNT
Sbjct: 456 GLVSGATIQSLLFALITIFTNWHKQALIARERVLEGNT 493

BLAST of Spg021595 vs. ExPASy Swiss-Prot
Match: F4HQ05 (Protein DETOXIFICATION 8 OS=Arabidopsis thaliana OX=3702 GN=DTX8 PE=3 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 4.3e-128
Identity = 230/444 (51.80%), Postives = 325/444 (73.20%), Query Frame = 0

Query: 12  LKKVSFLAAPIVIAFVLQYLLQVEIVIIVGHLDDELLLSGVSIATSFARATGFGLLLGMA 71
           +KKVSF+AAP+V     QYLLQV  +++ GHL DEL LS V+IATS    TGF L+ G+A
Sbjct: 36  MKKVSFMAAPMVAVAASQYLLQVISIVMAGHL-DELSLSAVAIATSLTNVTGFSLIFGLA 95

Query: 72  GALETLCGQAYGAEQYQKLGVYTYSCTISLILVCFPISILWLFTDKLLISIGQDPSISYV 131
           GALETLCGQA+GA Q++ +  YTY   + L+LVCFPIS+LW+F DKLL    QDP IS +
Sbjct: 96  GALETLCGQAFGAGQFRNISAYTYGSMLCLLLVCFPISLLWVFMDKLLELFHQDPLISQL 155

Query: 132 ARKYSVFLIPNLFAYAILQSLMRYLFTQSLIIPLLVCSFVTLSLHIPISWLLVFHFNLKV 191
           A +YS++LIP LF Y++LQS+ R+  +Q L++PL + S   L  H+P SWLLV+     +
Sbjct: 156 ACRYSIWLIPALFGYSVLQSMTRFFQSQGLVLPLFLSSLGALFFHVPFSWLLVYKLRFGI 215

Query: 192 VGAALALGISYWLNVILLGLYIFFSPSCKKTRAPFSRDAILSISEFFRLAVPSAVMTCLE 251
           VGAAL++G SYWLNV LL  ++  S   +K     +++  LS+ +F  LA+P+A+MTCLE
Sbjct: 216 VGAALSIGFSYWLNVGLLWAFMRDSALYRKNWNLRAQEIFLSMKQFITLAIPTAMMTCLE 275

Query: 252 WWSYEVILLLSGLLPNPKVETSVLSICFSITYLHFFIPYGLAATVSTRVSNELGAGNPEA 311
           WWS+E+++L+SGLLPN K+ETSVLSIC +++ LH+ I   + A  ST VSN+LGAGNP+A
Sbjct: 276 WWSFELLILMSGLLPNSKLETSVLSICLTMSSLHYVIVNAIGAAASTHVSNKLGAGNPKA 335

Query: 312 AKVAVRAVGILGIIESTIVSVTLFGCHNILGYAFTSDNQIAKHIASMWPLICLSIFIDSF 371
           A+ A  +   LG+I++ IVS++L+       Y F++++++A ++  + P +CLSI +DSF
Sbjct: 336 ARSAANSAIFLGMIDAAIVSISLYSYRRNWAYIFSNESEVADYVTQITPFLCLSIGVDSF 395

Query: 372 LAILTGVARGTGWQRLGAYVNLGSYYIVGIPMAAVLAFVAHLRVKGLWIGLVSGAVLQSF 431
           LA+L+GVARGTGWQ +GAY N+GSYY+VGIP+ ++L FV  LR KGLWIG++ G+ LQ+ 
Sbjct: 396 LAVLSGVARGTGWQHIGAYANIGSYYLVGIPVGSILCFVVKLRGKGLWIGILVGSTLQTI 455

Query: 432 LFALITIFTNWHKQALKARGRILE 456
           + AL+T FTNW ++  KAR R++E
Sbjct: 456 VLALVTFFTNWEQEVAKARDRVIE 478

BLAST of Spg021595 vs. ExPASy Swiss-Prot
Match: Q9SIA4 (Protein DETOXIFICATION 3 OS=Arabidopsis thaliana OX=3702 GN=DTX3 PE=3 SV=1)

HSP 1 Score: 456.1 bits (1172), Expect = 4.8e-127
Identity = 233/444 (52.48%), Postives = 313/444 (70.50%), Query Frame = 0

Query: 11  ELKKVSFLAAPIVIAFVLQYLLQVEIVIIVGHLDDELLLSGVSIATSFARATGFGLLLGM 70
           ELKKVS LAAP+    + QYLL V  V++ GH + EL LSGV++ATSF   +GF +L G+
Sbjct: 27  ELKKVSSLAAPMAAVTIAQYLLPVISVMVAGH-NGELQLSGVALATSFTNVSGFSILFGL 86

Query: 71  AGALETLCGQAYGAEQYQKLGVYTYSCTISLILVCFPISILWLFTDKLLISIGQDPSISY 130
           AGALETLCGQAYGA+QY+K+G YTYS T S I +C  IS+LW++ +KLLIS+GQDP IS 
Sbjct: 87  AGALETLCGQAYGAKQYEKIGTYTYSATASNIPICVLISVLWIYIEKLLISLGQDPDISR 146

Query: 131 VARKYSVFLIPNLFAYAILQSLMRYLFTQSLIIPLLVCSFVTLSLHIPISWLLVFHFNLK 190
           VA  Y+++LIP LFA+A    L R+L  Q L++PLL C+  TL  HIP+ W  V+ F L 
Sbjct: 147 VAGSYALWLIPALFAHAFFIPLTRFLLAQGLVLPLLYCTLTTLLFHIPVCWAFVYAFGLG 206

Query: 191 VVGAALALGISYWLNVILLGLYIFFSPSCKKTRAPFSRDAILSISEFFRLAVPSAVMTCL 250
             GAA+A+ +S+W  V++L  Y+ +S SC KTR   S D +  I +FF   VPSA M CL
Sbjct: 207 SNGAAMAISVSFWFYVVILSCYVRYSSSCDKTRVFVSSDFVSCIKQFFHFGVPSAAMVCL 266

Query: 251 EWWSYEVILLLSGLLPNPKVETSVLSICFSITYLHFFIPYGLAATVSTRVSNELGAGNPE 310
           EWW +E+++L SGLLPNPK+ETSVLSIC +   LH+ IP G+AA VSTRVSN+LGAG P+
Sbjct: 267 EWWLFELLILCSGLLPNPKLETSVLSICLTTASLHYVIPGGVAAAVSTRVSNKLGAGIPQ 326

Query: 311 AAKVAVRAVGILGIIESTIVSVTLFGCHNILGYAFTSDNQIAKHIASMWPLICLSIFIDS 370
            A+V+V A   L ++ES   S  LF C NI+GYAF++  ++  ++A++ PL+CLS  +D 
Sbjct: 327 VARVSVLAGLCLWLVESAFFSTLLFTCRNIIGYAFSNSKEVVDYVANLTPLLCLSFILDG 386

Query: 371 FLAILTGVARGTGWQRLGAYVNLGSYYIVGIPMAAVLAFVAHLRVKGLWIGLVSGAVLQS 430
           F A+L GVARG+GWQ +GA  N+ +YY+VG P+   LAF   L  KGLW G+V G+ +Q+
Sbjct: 387 FTAVLNGVARGSGWQHIGALNNVVAYYLVGAPVGVYLAFNRELNGKGLWCGVVVGSAVQA 446

Query: 431 FLFALITIFTNWHKQALKARGRIL 455
            + A +T   NW +QA KAR R++
Sbjct: 447 IILAFVTASINWKEQAEKARKRMV 469

BLAST of Spg021595 vs. ExPASy Swiss-Prot
Match: Q9SIA5 (Protein DETOXIFICATION 1 OS=Arabidopsis thaliana OX=3702 GN=DTX1 PE=2 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 5.3e-126
Identity = 234/444 (52.70%), Postives = 311/444 (70.05%), Query Frame = 0

Query: 11  ELKKVSFLAAPIVIAFVLQYLLQVEIVIIVGHLDDELLLSGVSIATSFARATGFGLLLGM 70
           ELK+VS LAAP+    + QYLL V  V++ GH + EL LSGV++A SF   TGF ++ G+
Sbjct: 27  ELKRVSRLAAPMATVTIAQYLLPVISVMVAGH-NGELQLSGVALANSFTNVTGFSIMCGL 86

Query: 71  AGALETLCGQAYGAEQYQKLGVYTYSCTISLILVCFPISILWLFTDKLLISIGQDPSISY 130
            GALETLCGQAYGA+QY+K+G Y YS   S I +CF ISILWL+ +K+LIS+GQDP IS 
Sbjct: 87  VGALETLCGQAYGAKQYEKIGTYAYSAIASNIPICFLISILWLYIEKILISLGQDPEISR 146

Query: 131 VARKYSVFLIPNLFAYAILQSLMRYLFTQSLIIPLLVCSFVTLSLHIPISWLLVFHFNLK 190
           +A  Y+ +LIP LF  AI+  L R+L TQ L+IPLL  +  TL  H+ + W LVF F L 
Sbjct: 147 IAGSYAFWLIPALFGQAIVIPLSRFLLTQGLVIPLLFTAVTTLLFHVLVCWTLVFLFGLG 206

Query: 191 VVGAALALGISYWLNVILLGLYIFFSPSCKKTRAPFSRDAILSISEFFRLAVPSAVMTCL 250
             G A+A  +S+W   ++L  Y+ FS SC+KTR   SRD + SI +FF+  +PSA M CL
Sbjct: 207 CNGPAMATSVSFWFYAVILSCYVRFSSSCEKTRGFVSRDFVSSIKQFFQYGIPSAAMICL 266

Query: 251 EWWSYEVILLLSGLLPNPKVETSVLSICFSITYLHFFIPYGLAATVSTRVSNELGAGNPE 310
           EWW +E+++L SGLLPNPK+ETSVLSIC +I  LH+ I  G+AA VSTRVSN LGAGNP+
Sbjct: 267 EWWLFEILILCSGLLPNPKLETSVLSICLTIETLHYVISAGVAAAVSTRVSNNLGAGNPQ 326

Query: 311 AAKVAVRAVGILGIIESTIVSVTLFGCHNILGYAFTSDNQIAKHIASMWPLICLSIFIDS 370
            A+V+V A   L I+ES   S+ LF C NI+GYAF++  ++  ++A + PL+CLS  +D 
Sbjct: 327 VARVSVLAGLCLWIVESAFFSILLFTCRNIIGYAFSNSKEVLDYVADLTPLLCLSFILDG 386

Query: 371 FLAILTGVARGTGWQRLGAYVNLGSYYIVGIPMAAVLAFVAHLRVKGLWIGLVSGAVLQS 430
           F A+L GVARG+GWQ +GA+ N  SYY+VG P+   LAF   L  KGLW G+V G+ +Q+
Sbjct: 387 FTAVLNGVARGSGWQHIGAWNNTVSYYLVGAPVGIYLAFSRELNGKGLWCGVVVGSTVQA 446

Query: 431 FLFALITIFTNWHKQALKARGRIL 455
            + A++T   NW +QA KAR RI+
Sbjct: 447 TILAIVTASINWKEQAEKARKRIV 469

BLAST of Spg021595 vs. ExPASy Swiss-Prot
Match: Q9SIA1 (Protein DETOXIFICATION 5 OS=Arabidopsis thaliana OX=3702 GN=DTX5 PE=3 SV=2)

HSP 1 Score: 446.4 bits (1147), Expect = 3.8e-124
Identity = 229/450 (50.89%), Postives = 314/450 (69.78%), Query Frame = 0

Query: 5   WRVYEKELKKVSFLAAPIVIAFVLQYLLQVEIVIIVGHLDDELLLSGVSIATSFARATGF 64
           W  +  ELK VS +AAP+    V QYLL V  V++ GH   EL LSGV++AT+FA  +GF
Sbjct: 24  WMNFTAELKNVSSMAAPMATVTVSQYLLPVISVMVAGHC-GELQLSGVTLATAFANVSGF 83

Query: 65  GLLLGMAGALETLCGQAYGAEQYQKLGVYTYSCTISLILVCFPISILWLFTDKLLISIGQ 124
           G++ G+ GALETLCGQAYGA+QY K+G YT+S  +S + +   ISILW + DKL +S+GQ
Sbjct: 84  GIMYGLVGALETLCGQAYGAKQYTKIGTYTFSAIVSNVPIVVLISILWFYMDKLFVSLGQ 143

Query: 125 DPSISYVARKYSVFLIPNLFAYAILQSLMRYLFTQSLIIPLLVCSFVTLSLHIPISWLLV 184
           DP IS VA  Y+V LIP L A A+ Q L R+L TQ L++PLL C+  TL  HIP+  +LV
Sbjct: 144 DPDISKVAGSYAVCLIPALLAQAVQQPLTRFLQTQGLVLPLLYCAITTLLFHIPVCLILV 203

Query: 185 FHFNLKVVGAALALGISYWLNVILLGLYIFFSPSCKKTRAPFSRDAILSISEFFRLAVPS 244
           + F L   GAALA+G+SYW NV++L LY+ FS +C+KTR   S D +LS+ +FF+  +PS
Sbjct: 204 YAFGLGSNGAALAIGLSYWFNVLILALYVRFSSACEKTRGFVSDDFVLSVKQFFQYGIPS 263

Query: 245 AVMTCLEWWSYEVILLLSGLLPNPKVETSVLSICFSITYLHFFIPYGLAATVSTRVSNEL 304
           A MT +EW  +E+++L SGLLPNPK+ETSVLSIC + + LH  IP G+ A  STR+SNEL
Sbjct: 264 AAMTTIEWSLFELLILSSGLLPNPKLETSVLSICLTTSSLHCVIPMGIGAAGSTRISNEL 323

Query: 305 GAGNPEAAKVAVRAVGILGIIESTIVSVTLFGCHNILGYAFTSDNQIAKHIASMWPLICL 364
           GAGNPE A++AV A   L  +E+TI S  LF C NI GYAF++  ++  ++  +  L+CL
Sbjct: 324 GAGNPEVARLAVFAGIFLWFLEATICSTLLFTCKNIFGYAFSNSKEVVDYVTELSSLLCL 383

Query: 365 SIFIDSFLAILTGVARGTGWQRLGAYVNLGSYYIVGIPMAAVLAFVAHLRVKGLWIGLVS 424
           S  +D F ++L GVARG+GWQ +GA+ N+ +YY++G P+   L F  H+  KGLWIG++ 
Sbjct: 384 SFMVDGFSSVLDGVARGSGWQNIGAWANVVAYYLLGAPVGFFLGFWGHMNGKGLWIGVIV 443

Query: 425 GAVLQSFLFALITIFTNWHKQALKARGRIL 455
           G+  Q  + A++T   +W +QA KAR RI+
Sbjct: 444 GSTAQGIILAIVTACLSWEEQAAKARERIV 472

BLAST of Spg021595 vs. ExPASy Swiss-Prot
Match: Q8RWF5 (Protein DETOXIFICATION 6 OS=Arabidopsis thaliana OX=3702 GN=DTX6 PE=2 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 1.9e-123
Identity = 230/451 (51.00%), Postives = 315/451 (69.84%), Query Frame = 0

Query: 5   WRV-YEKELKKVSFLAAPIVIAFVLQYLLQVEIVIIVGHLDDELLLSGVSIATSFARATG 64
           WR+ +  ELK +S +A P+    V QYLL V  V++ GH   EL LSGV++ATSF   +G
Sbjct: 23  WRMNFTAELKNLSRMALPMATVTVAQYLLPVISVMVAGH-RSELQLSGVALATSFTNVSG 82

Query: 65  FGLLLGMAGALETLCGQAYGAEQYQKLGVYTYSCTISLILVCFPISILWLFTDKLLISIG 124
           F ++ G+AGALETLCGQAYGA+QY K+G YT+S  +S + +   ISILW + DKL +S+G
Sbjct: 83  FSVMFGLAGALETLCGQAYGAKQYAKIGTYTFSAIVSNVPIVVLISILWFYMDKLFVSLG 142

Query: 125 QDPSISYVARKYSVFLIPNLFAYAILQSLMRYLFTQSLIIPLLVCSFVTLSLHIPISWLL 184
           QDP IS VA  Y+V LIP L A A+ Q L R+L TQ L++PLL C+  TL  HIP+  +L
Sbjct: 143 QDPDISKVAGSYAVCLIPALLAQAVQQPLTRFLQTQGLVLPLLYCAITTLLFHIPVCLIL 202

Query: 185 VFHFNLKVVGAALALGISYWLNVILLGLYIFFSPSCKKTRAPFSRDAILSISEFFRLAVP 244
           V+ F L   GAALA+G+SYW NV++L LY+ FS SC+KTR   S D +LS+ +FF+  +P
Sbjct: 203 VYAFGLGSNGAALAIGLSYWFNVLILALYVRFSSSCEKTRGFVSDDFVLSVKQFFQYGIP 262

Query: 245 SAVMTCLEWWSYEVILLLSGLLPNPKVETSVLSICFSITYLHFFIPYGLAATVSTRVSNE 304
           SA MT +EW  +E ++L SGLLPNPK+ETSVLSIC + + LH+ IP G+ A  S RVSNE
Sbjct: 263 SAAMTTIEWSLFEFLILSSGLLPNPKLETSVLSICLTTSSLHYVIPMGIGAAGSIRVSNE 322

Query: 305 LGAGNPEAAKVAVRAVGILGIIESTIVSVTLFGCHNILGYAFTSDNQIAKHIASMWPLIC 364
           LGAGNPE A++AV A   L  +E+TI S  LF C +I GYAF++  ++  ++  + PL+C
Sbjct: 323 LGAGNPEVARLAVFAGIFLWFLEATICSTLLFICRDIFGYAFSNSKEVVDYVTELSPLLC 382

Query: 365 LSIFIDSFLAILTGVARGTGWQRLGAYVNLGSYYIVGIPMAAVLAFVAHLRVKGLWIGLV 424
           +S  +D F A+L GVARG+GWQ +GA+ N+ +YY++G P+   L F  H+  KGLWIG+V
Sbjct: 383 ISFLVDGFSAVLGGVARGSGWQHIGAWANVVAYYLLGAPVGLFLGFWCHMNGKGLWIGVV 442

Query: 425 SGAVLQSFLFALITIFTNWHKQALKARGRIL 455
            G+  Q  + A++T   +W++QA KAR RI+
Sbjct: 443 VGSTAQGIILAIVTACMSWNEQAAKARQRIV 472

BLAST of Spg021595 vs. ExPASy TrEMBL
Match: A0A6J1IA18 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111472575 PE=3 SV=1)

HSP 1 Score: 758.8 bits (1958), Expect = 1.3e-215
Identity = 386/458 (84.28%), Postives = 413/458 (90.17%), Query Frame = 0

Query: 1   MREKWRVYEKELKKVSFLAAPIVIAFVLQYLLQVEIVIIVGHLDDELLLSGVSIATSFAR 60
           MREKWRVY+KE+KKVSFLAAPI+  FVLQYLLQV  VIIVGHL DELLLSG+SIA+SF R
Sbjct: 27  MREKWRVYQKEVKKVSFLAAPIITVFVLQYLLQVVTVIIVGHLGDELLLSGISIASSFVR 86

Query: 61  ATGFGLLLGMAGALETLCGQAYGAEQYQKLGVYTYSCTISLILVCFPISILWLFTDKLLI 120
            TGF LLLGMAGALETLCGQAYGA+QYQK+GVYTYSC I L+L CFPISILW FTDKLLI
Sbjct: 87  VTGFALLLGMAGALETLCGQAYGAQQYQKIGVYTYSCMICLLLACFPISILWFFTDKLLI 146

Query: 121 SIGQDPSISYVARKYSVFLIPNLFAYAILQSLMRYLFTQSLIIPLLVCSFVTLSLHIPIS 180
           SIGQDPSIS +ARKYS+FLIPNLFA+AILQSLMRYL TQSLI+PLL CS  TLS HIPI 
Sbjct: 147 SIGQDPSISSIARKYSIFLIPNLFAHAILQSLMRYLLTQSLILPLLFCSLATLSFHIPIC 206

Query: 181 WLLVFHFNLKVVGAALALGISYWLNVILLGLYIFFSPSCKKTRAPFSRDAILSISEFFRL 240
           WLLVFHFNLKVVGAALALG+SYWLNVILLGLYI FSPSC KTRAPFS +AI SI +FFRL
Sbjct: 207 WLLVFHFNLKVVGAALALGMSYWLNVILLGLYIVFSPSCNKTRAPFSMEAISSIGKFFRL 266

Query: 241 AVPSAVMTCLEWWSYEVILLLSGLLPNPKVETSVLSICFSITYLHFFIPYGLAATVSTRV 300
           A+PSAVM C EWWSYE+ILLLSGLLPNPKVE S+LSICFS+TYLH+FIPYGL AT STRV
Sbjct: 267 AIPSAVMVCFEWWSYEIILLLSGLLPNPKVEASMLSICFSMTYLHYFIPYGLGATASTRV 326

Query: 301 SNELGAGNPEAAKVAVRAVGILGIIESTIVSVTLFGCHNILGYAFTSDNQIAKHIASMWP 360
           SNELGAGNPEAAKVAV+ VG+LGIIES  +S  LFGCHN+LGYAFTSD QIA HIAS WP
Sbjct: 327 SNELGAGNPEAAKVAVKVVGVLGIIESVTMSAALFGCHNMLGYAFTSDTQIANHIASFWP 386

Query: 361 LICLSIFIDSFLAILTGVARGTGWQRLGAYVNLGSYYIVGIPMAAVLAFVAHLRVKGLWI 420
           LICLSI IDSFL IL+GVARG GWQR+GAYVNLGSYYIVGIPMAAVLAFV HLRVKGLWI
Sbjct: 387 LICLSILIDSFLGILSGVARGCGWQRVGAYVNLGSYYIVGIPMAAVLAFVVHLRVKGLWI 446

Query: 421 GLVSGAVLQSFLFALITIFTNWHKQALKARGRILEGNT 459
           GLVSGA LQSFLFALIT FTNWHKQALKAR R+LE NT
Sbjct: 447 GLVSGATLQSFLFALITTFTNWHKQALKARERVLEENT 484

BLAST of Spg021595 vs. ExPASy TrEMBL
Match: A0A6J1ECE1 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111432792 PE=3 SV=1)

HSP 1 Score: 756.9 bits (1953), Expect = 4.9e-215
Identity = 385/458 (84.06%), Postives = 413/458 (90.17%), Query Frame = 0

Query: 1   MREKWRVYEKELKKVSFLAAPIVIAFVLQYLLQVEIVIIVGHLDDELLLSGVSIATSFAR 60
           MREKWRVY+KE+KKVSFLAAPI+I FVLQYLLQV  +IIVGHL DELLLSG+SIA+SF R
Sbjct: 27  MREKWRVYQKEVKKVSFLAAPIIIVFVLQYLLQVVTIIIVGHLGDELLLSGISIASSFVR 86

Query: 61  ATGFGLLLGMAGALETLCGQAYGAEQYQKLGVYTYSCTISLILVCFPISILWLFTDKLLI 120
            TGF LLLGMAGALETLCGQAYGA+QYQK+GVYTYSC I L+L CFPISI+W FTDKLLI
Sbjct: 87  VTGFALLLGMAGALETLCGQAYGAQQYQKIGVYTYSCMICLLLACFPISIVWFFTDKLLI 146

Query: 121 SIGQDPSISYVARKYSVFLIPNLFAYAILQSLMRYLFTQSLIIPLLVCSFVTLSLHIPIS 180
           SIGQDPSIS +ARKYS+FLIPNLFA+AILQSLMRYL TQSLI+PLL CS  TLS HIPI 
Sbjct: 147 SIGQDPSISSIARKYSLFLIPNLFAHAILQSLMRYLLTQSLILPLLFCSLATLSFHIPIC 206

Query: 181 WLLVFHFNLKVVGAALALGISYWLNVILLGLYIFFSPSCKKTRAPFSRDAILSISEFFRL 240
           WLLVFHFNLKVVGAALALG+SYWLNVILLGLYI FSPSC KT+A FS +AI SI +FFRL
Sbjct: 207 WLLVFHFNLKVVGAALALGMSYWLNVILLGLYIVFSPSCNKTQATFSMEAISSIGKFFRL 266

Query: 241 AVPSAVMTCLEWWSYEVILLLSGLLPNPKVETSVLSICFSITYLHFFIPYGLAATVSTRV 300
           A+PSAVM C EWWSYE+ILLLSGLLPNPKVE SVLSICFS+TYLH+FIPYGL AT STRV
Sbjct: 267 AIPSAVMVCFEWWSYEIILLLSGLLPNPKVEASVLSICFSVTYLHYFIPYGLGATASTRV 326

Query: 301 SNELGAGNPEAAKVAVRAVGILGIIESTIVSVTLFGCHNILGYAFTSDNQIAKHIASMWP 360
           SNELGAGNPEAAKVAV+ VG+LGIIES  VS  LFGCHN+LGYAFTSD QIA HIAS WP
Sbjct: 327 SNELGAGNPEAAKVAVKVVGVLGIIESVTVSAALFGCHNMLGYAFTSDTQIANHIASFWP 386

Query: 361 LICLSIFIDSFLAILTGVARGTGWQRLGAYVNLGSYYIVGIPMAAVLAFVAHLRVKGLWI 420
           LICLSI IDSFL IL+GVARG GWQR+GAYVNLGSYYIVGIPMAAVLAFV HLRVKGLWI
Sbjct: 387 LICLSILIDSFLGILSGVARGCGWQRVGAYVNLGSYYIVGIPMAAVLAFVVHLRVKGLWI 446

Query: 421 GLVSGAVLQSFLFALITIFTNWHKQALKARGRILEGNT 459
           GLVSGA LQSFLFALIT FTNWHKQALKAR R+LE NT
Sbjct: 447 GLVSGATLQSFLFALITTFTNWHKQALKARERVLEENT 484

BLAST of Spg021595 vs. ExPASy TrEMBL
Match: A0A6J1CLS8 (Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111012127 PE=3 SV=1)

HSP 1 Score: 753.8 bits (1945), Expect = 4.1e-214
Identity = 393/458 (85.81%), Postives = 410/458 (89.52%), Query Frame = 0

Query: 1   MREKWRVYEKELKKVSFLAAPIVIAFVLQYLLQVEIVIIVGHLDDELLLSGVSIATSFAR 60
           MREKWRVY  ELKKVS +AAPI  A VLQYLLQV  V+IVGHL DELLLSGVSIATSF R
Sbjct: 36  MREKWRVYPTELKKVSLIAAPITTALVLQYLLQVVTVVIVGHLGDELLLSGVSIATSFVR 95

Query: 61  ATGFGLLLGMAGALETLCGQAYGAEQYQKLGVYTYSCTISLILVCFPISILWLFTDKLLI 120
            TGF LLLGMAGALETLCGQAYGAEQYQKLGVYTYSC ISLILVCFPIS+LW FTDKLLI
Sbjct: 96  VTGFSLLLGMAGALETLCGQAYGAEQYQKLGVYTYSCMISLILVCFPISVLWFFTDKLLI 155

Query: 121 SIGQDPSISYVARKYSVFLIPNLFAYAILQSLMRYLFTQSLIIPLLVCSFVTLSLHIPIS 180
           SIGQDPSIS VARKYSVFLIPNLFA AILQSL+RY  TQSLI+PLL  SF TL LHIPI 
Sbjct: 156 SIGQDPSISSVARKYSVFLIPNLFACAILQSLLRYFLTQSLILPLLFSSFATLCLHIPIC 215

Query: 181 WLLVFHFNLKVVGAALALGISYWLNVILLGLYIFFSPSCKKTRAPFSRDAILSISEFFRL 240
           WL VFHF L+VVGAALALGISYWLNVILL  Y+FFSPSC KTRAP SR+AI SI +FFRL
Sbjct: 216 WLFVFHFKLRVVGAALALGISYWLNVILLASYVFFSPSCNKTRAPLSREAISSIGQFFRL 275

Query: 241 AVPSAVMTCLEWWSYEVILLLSGLLPNPKVETSVLSICFSITYLHFFIPYGLAATVSTRV 300
           AVPSAVM CLEWWSYEVILLLSGLLPNPKVE SVLSICFSITYLH+FIPYGL ATVSTRV
Sbjct: 276 AVPSAVMVCLEWWSYEVILLLSGLLPNPKVEASVLSICFSITYLHYFIPYGLGATVSTRV 335

Query: 301 SNELGAGNPEAAKVAVRAVGILGIIESTIVSVTLFGCHNILGYAFTSDNQIAKHIASMWP 360
           SNELGAGNPEAAKVAV+ VG LGIIES  VSV LFGC NILGYAFT+D QIA HIASMWP
Sbjct: 336 SNELGAGNPEAAKVAVKVVGALGIIESITVSVILFGCRNILGYAFTNDTQIADHIASMWP 395

Query: 361 LICLSIFIDSFLAILTGVARGTGWQRLGAYVNLGSYYIVGIPMAAVLAFVAHLRVKGLWI 420
           LICLSI ID+FL IL+GVARGTGWQ+LGAYVNLGSYYIVGIPMAAVLAFVAHLRVKGLWI
Sbjct: 396 LICLSILIDTFLGILSGVARGTGWQQLGAYVNLGSYYIVGIPMAAVLAFVAHLRVKGLWI 455

Query: 421 GLVSGAVLQSFLFALITIFTNWHKQALKARGRILEGNT 459
           GLVSGA +QS LFALITIFTNWHKQAL AR R+LEGNT
Sbjct: 456 GLVSGATIQSLLFALITIFTNWHKQALIARERVLEGNT 493

BLAST of Spg021595 vs. ExPASy TrEMBL
Match: A0A6J1CK03 (Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111012127 PE=3 SV=1)

HSP 1 Score: 735.7 bits (1898), Expect = 1.2e-208
Identity = 383/446 (85.87%), Postives = 400/446 (89.69%), Query Frame = 0

Query: 1   MREKWRVYEKELKKVSFLAAPIVIAFVLQYLLQVEIVIIVGHLDDELLLSGVSIATSFAR 60
           MREKWRVY  ELKKVS +AAPI  A VLQYLLQV  V+IVGHL DELLLSGVSIATSF R
Sbjct: 36  MREKWRVYPTELKKVSLIAAPITTALVLQYLLQVVTVVIVGHLGDELLLSGVSIATSFVR 95

Query: 61  ATGFGLLLGMAGALETLCGQAYGAEQYQKLGVYTYSCTISLILVCFPISILWLFTDKLLI 120
            TGF LLLGMAGALETLCGQAYGAEQYQKLGVYTYSC ISLILVCFPIS+LW FTDKLLI
Sbjct: 96  VTGFSLLLGMAGALETLCGQAYGAEQYQKLGVYTYSCMISLILVCFPISVLWFFTDKLLI 155

Query: 121 SIGQDPSISYVARKYSVFLIPNLFAYAILQSLMRYLFTQSLIIPLLVCSFVTLSLHIPIS 180
           SIGQDPSIS VARKYSVFLIPNLFA AILQSL+RY  TQSLI+PLL  SF TL LHIPI 
Sbjct: 156 SIGQDPSISSVARKYSVFLIPNLFACAILQSLLRYFLTQSLILPLLFSSFATLCLHIPIC 215

Query: 181 WLLVFHFNLKVVGAALALGISYWLNVILLGLYIFFSPSCKKTRAPFSRDAILSISEFFRL 240
           WL VFHF L+VVGAALALGISYWLNVILL  Y+FFSPSC KTRAP SR+AI SI +FFRL
Sbjct: 216 WLFVFHFKLRVVGAALALGISYWLNVILLASYVFFSPSCNKTRAPLSREAISSIGQFFRL 275

Query: 241 AVPSAVMTCLEWWSYEVILLLSGLLPNPKVETSVLSICFSITYLHFFIPYGLAATVSTRV 300
           AVPSAVM CLEWWSYEVILLLSGLLPNPKVE SVLSICFSITYLH+FIPYGL ATVSTRV
Sbjct: 276 AVPSAVMVCLEWWSYEVILLLSGLLPNPKVEASVLSICFSITYLHYFIPYGLGATVSTRV 335

Query: 301 SNELGAGNPEAAKVAVRAVGILGIIESTIVSVTLFGCHNILGYAFTSDNQIAKHIASMWP 360
           SNELGAGNPEAAKVAV+ VG LGIIES  VSV LFGC NILGYAFT+D QIA HIASMWP
Sbjct: 336 SNELGAGNPEAAKVAVKVVGALGIIESITVSVILFGCRNILGYAFTNDTQIADHIASMWP 395

Query: 361 LICLSIFIDSFLAILTGVARGTGWQRLGAYVNLGSYYIVGIPMAAVLAFVAHLRVKGLWI 420
           LICLSI ID+FL IL+GVARGTGWQ+LGAYVNLGSYYIVGIPMAAVLAFVAHLRVKGLWI
Sbjct: 396 LICLSILIDTFLGILSGVARGTGWQQLGAYVNLGSYYIVGIPMAAVLAFVAHLRVKGLWI 455

Query: 421 GLVSGAVLQSFLFALITIFTNWHKQA 447
           GLVSGA +QS LFALITIFTNWHKQ+
Sbjct: 456 GLVSGATIQSLLFALITIFTNWHKQS 481

BLAST of Spg021595 vs. ExPASy TrEMBL
Match: A0A6J1CL18 (Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111012127 PE=3 SV=1)

HSP 1 Score: 735.3 bits (1897), Expect = 1.5e-208
Identity = 383/445 (86.07%), Postives = 399/445 (89.66%), Query Frame = 0

Query: 1   MREKWRVYEKELKKVSFLAAPIVIAFVLQYLLQVEIVIIVGHLDDELLLSGVSIATSFAR 60
           MREKWRVY  ELKKVS +AAPI  A VLQYLLQV  V+IVGHL DELLLSGVSIATSF R
Sbjct: 36  MREKWRVYPTELKKVSLIAAPITTALVLQYLLQVVTVVIVGHLGDELLLSGVSIATSFVR 95

Query: 61  ATGFGLLLGMAGALETLCGQAYGAEQYQKLGVYTYSCTISLILVCFPISILWLFTDKLLI 120
            TGF LLLGMAGALETLCGQAYGAEQYQKLGVYTYSC ISLILVCFPIS+LW FTDKLLI
Sbjct: 96  VTGFSLLLGMAGALETLCGQAYGAEQYQKLGVYTYSCMISLILVCFPISVLWFFTDKLLI 155

Query: 121 SIGQDPSISYVARKYSVFLIPNLFAYAILQSLMRYLFTQSLIIPLLVCSFVTLSLHIPIS 180
           SIGQDPSIS VARKYSVFLIPNLFA AILQSL+RY  TQSLI+PLL  SF TL LHIPI 
Sbjct: 156 SIGQDPSISSVARKYSVFLIPNLFACAILQSLLRYFLTQSLILPLLFSSFATLCLHIPIC 215

Query: 181 WLLVFHFNLKVVGAALALGISYWLNVILLGLYIFFSPSCKKTRAPFSRDAILSISEFFRL 240
           WL VFHF L+VVGAALALGISYWLNVILL  Y+FFSPSC KTRAP SR+AI SI +FFRL
Sbjct: 216 WLFVFHFKLRVVGAALALGISYWLNVILLASYVFFSPSCNKTRAPLSREAISSIGQFFRL 275

Query: 241 AVPSAVMTCLEWWSYEVILLLSGLLPNPKVETSVLSICFSITYLHFFIPYGLAATVSTRV 300
           AVPSAVM CLEWWSYEVILLLSGLLPNPKVE SVLSICFSITYLH+FIPYGL ATVSTRV
Sbjct: 276 AVPSAVMVCLEWWSYEVILLLSGLLPNPKVEASVLSICFSITYLHYFIPYGLGATVSTRV 335

Query: 301 SNELGAGNPEAAKVAVRAVGILGIIESTIVSVTLFGCHNILGYAFTSDNQIAKHIASMWP 360
           SNELGAGNPEAAKVAV+ VG LGIIES  VSV LFGC NILGYAFT+D QIA HIASMWP
Sbjct: 336 SNELGAGNPEAAKVAVKVVGALGIIESITVSVILFGCRNILGYAFTNDTQIADHIASMWP 395

Query: 361 LICLSIFIDSFLAILTGVARGTGWQRLGAYVNLGSYYIVGIPMAAVLAFVAHLRVKGLWI 420
           LICLSI ID+FL IL+GVARGTGWQ+LGAYVNLGSYYIVGIPMAAVLAFVAHLRVKGLWI
Sbjct: 396 LICLSILIDTFLGILSGVARGTGWQQLGAYVNLGSYYIVGIPMAAVLAFVAHLRVKGLWI 455

Query: 421 GLVSGAVLQSFLFALITIFTNWHKQ 446
           GLVSGA +QS LFALITIFTNWHKQ
Sbjct: 456 GLVSGATIQSLLFALITIFTNWHKQ 480

BLAST of Spg021595 vs. TAIR 10
Match: AT1G66780.1 (MATE efflux family protein )

HSP 1 Score: 459.5 bits (1181), Expect = 3.1e-129
Identity = 230/444 (51.80%), Postives = 325/444 (73.20%), Query Frame = 0

Query: 12  LKKVSFLAAPIVIAFVLQYLLQVEIVIIVGHLDDELLLSGVSIATSFARATGFGLLLGMA 71
           +KKVSF+AAP+V     QYLLQV  +++ GHL DEL LS V+IATS    TGF L+ G+A
Sbjct: 36  MKKVSFMAAPMVAVAASQYLLQVISIVMAGHL-DELSLSAVAIATSLTNVTGFSLIFGLA 95

Query: 72  GALETLCGQAYGAEQYQKLGVYTYSCTISLILVCFPISILWLFTDKLLISIGQDPSISYV 131
           GALETLCGQA+GA Q++ +  YTY   + L+LVCFPIS+LW+F DKLL    QDP IS +
Sbjct: 96  GALETLCGQAFGAGQFRNISAYTYGSMLCLLLVCFPISLLWVFMDKLLELFHQDPLISQL 155

Query: 132 ARKYSVFLIPNLFAYAILQSLMRYLFTQSLIIPLLVCSFVTLSLHIPISWLLVFHFNLKV 191
           A +YS++LIP LF Y++LQS+ R+  +Q L++PL + S   L  H+P SWLLV+     +
Sbjct: 156 ACRYSIWLIPALFGYSVLQSMTRFFQSQGLVLPLFLSSLGALFFHVPFSWLLVYKLRFGI 215

Query: 192 VGAALALGISYWLNVILLGLYIFFSPSCKKTRAPFSRDAILSISEFFRLAVPSAVMTCLE 251
           VGAAL++G SYWLNV LL  ++  S   +K     +++  LS+ +F  LA+P+A+MTCLE
Sbjct: 216 VGAALSIGFSYWLNVGLLWAFMRDSALYRKNWNLRAQEIFLSMKQFITLAIPTAMMTCLE 275

Query: 252 WWSYEVILLLSGLLPNPKVETSVLSICFSITYLHFFIPYGLAATVSTRVSNELGAGNPEA 311
           WWS+E+++L+SGLLPN K+ETSVLSIC +++ LH+ I   + A  ST VSN+LGAGNP+A
Sbjct: 276 WWSFELLILMSGLLPNSKLETSVLSICLTMSSLHYVIVNAIGAAASTHVSNKLGAGNPKA 335

Query: 312 AKVAVRAVGILGIIESTIVSVTLFGCHNILGYAFTSDNQIAKHIASMWPLICLSIFIDSF 371
           A+ A  +   LG+I++ IVS++L+       Y F++++++A ++  + P +CLSI +DSF
Sbjct: 336 ARSAANSAIFLGMIDAAIVSISLYSYRRNWAYIFSNESEVADYVTQITPFLCLSIGVDSF 395

Query: 372 LAILTGVARGTGWQRLGAYVNLGSYYIVGIPMAAVLAFVAHLRVKGLWIGLVSGAVLQSF 431
           LA+L+GVARGTGWQ +GAY N+GSYY+VGIP+ ++L FV  LR KGLWIG++ G+ LQ+ 
Sbjct: 396 LAVLSGVARGTGWQHIGAYANIGSYYLVGIPVGSILCFVVKLRGKGLWIGILVGSTLQTI 455

Query: 432 LFALITIFTNWHKQALKARGRILE 456
           + AL+T FTNW ++  KAR R++E
Sbjct: 456 VLALVTFFTNWEQEVAKARDRVIE 478

BLAST of Spg021595 vs. TAIR 10
Match: AT2G04050.1 (MATE efflux family protein )

HSP 1 Score: 456.1 bits (1172), Expect = 3.4e-128
Identity = 233/444 (52.48%), Postives = 313/444 (70.50%), Query Frame = 0

Query: 11  ELKKVSFLAAPIVIAFVLQYLLQVEIVIIVGHLDDELLLSGVSIATSFARATGFGLLLGM 70
           ELKKVS LAAP+    + QYLL V  V++ GH + EL LSGV++ATSF   +GF +L G+
Sbjct: 27  ELKKVSSLAAPMAAVTIAQYLLPVISVMVAGH-NGELQLSGVALATSFTNVSGFSILFGL 86

Query: 71  AGALETLCGQAYGAEQYQKLGVYTYSCTISLILVCFPISILWLFTDKLLISIGQDPSISY 130
           AGALETLCGQAYGA+QY+K+G YTYS T S I +C  IS+LW++ +KLLIS+GQDP IS 
Sbjct: 87  AGALETLCGQAYGAKQYEKIGTYTYSATASNIPICVLISVLWIYIEKLLISLGQDPDISR 146

Query: 131 VARKYSVFLIPNLFAYAILQSLMRYLFTQSLIIPLLVCSFVTLSLHIPISWLLVFHFNLK 190
           VA  Y+++LIP LFA+A    L R+L  Q L++PLL C+  TL  HIP+ W  V+ F L 
Sbjct: 147 VAGSYALWLIPALFAHAFFIPLTRFLLAQGLVLPLLYCTLTTLLFHIPVCWAFVYAFGLG 206

Query: 191 VVGAALALGISYWLNVILLGLYIFFSPSCKKTRAPFSRDAILSISEFFRLAVPSAVMTCL 250
             GAA+A+ +S+W  V++L  Y+ +S SC KTR   S D +  I +FF   VPSA M CL
Sbjct: 207 SNGAAMAISVSFWFYVVILSCYVRYSSSCDKTRVFVSSDFVSCIKQFFHFGVPSAAMVCL 266

Query: 251 EWWSYEVILLLSGLLPNPKVETSVLSICFSITYLHFFIPYGLAATVSTRVSNELGAGNPE 310
           EWW +E+++L SGLLPNPK+ETSVLSIC +   LH+ IP G+AA VSTRVSN+LGAG P+
Sbjct: 267 EWWLFELLILCSGLLPNPKLETSVLSICLTTASLHYVIPGGVAAAVSTRVSNKLGAGIPQ 326

Query: 311 AAKVAVRAVGILGIIESTIVSVTLFGCHNILGYAFTSDNQIAKHIASMWPLICLSIFIDS 370
            A+V+V A   L ++ES   S  LF C NI+GYAF++  ++  ++A++ PL+CLS  +D 
Sbjct: 327 VARVSVLAGLCLWLVESAFFSTLLFTCRNIIGYAFSNSKEVVDYVANLTPLLCLSFILDG 386

Query: 371 FLAILTGVARGTGWQRLGAYVNLGSYYIVGIPMAAVLAFVAHLRVKGLWIGLVSGAVLQS 430
           F A+L GVARG+GWQ +GA  N+ +YY+VG P+   LAF   L  KGLW G+V G+ +Q+
Sbjct: 387 FTAVLNGVARGSGWQHIGALNNVVAYYLVGAPVGVYLAFNRELNGKGLWCGVVVGSAVQA 446

Query: 431 FLFALITIFTNWHKQALKARGRIL 455
            + A +T   NW +QA KAR R++
Sbjct: 447 IILAFVTASINWKEQAEKARKRMV 469

BLAST of Spg021595 vs. TAIR 10
Match: AT2G04040.1 (MATE efflux family protein )

HSP 1 Score: 452.6 bits (1163), Expect = 3.8e-127
Identity = 234/444 (52.70%), Postives = 311/444 (70.05%), Query Frame = 0

Query: 11  ELKKVSFLAAPIVIAFVLQYLLQVEIVIIVGHLDDELLLSGVSIATSFARATGFGLLLGM 70
           ELK+VS LAAP+    + QYLL V  V++ GH + EL LSGV++A SF   TGF ++ G+
Sbjct: 27  ELKRVSRLAAPMATVTIAQYLLPVISVMVAGH-NGELQLSGVALANSFTNVTGFSIMCGL 86

Query: 71  AGALETLCGQAYGAEQYQKLGVYTYSCTISLILVCFPISILWLFTDKLLISIGQDPSISY 130
            GALETLCGQAYGA+QY+K+G Y YS   S I +CF ISILWL+ +K+LIS+GQDP IS 
Sbjct: 87  VGALETLCGQAYGAKQYEKIGTYAYSAIASNIPICFLISILWLYIEKILISLGQDPEISR 146

Query: 131 VARKYSVFLIPNLFAYAILQSLMRYLFTQSLIIPLLVCSFVTLSLHIPISWLLVFHFNLK 190
           +A  Y+ +LIP LF  AI+  L R+L TQ L+IPLL  +  TL  H+ + W LVF F L 
Sbjct: 147 IAGSYAFWLIPALFGQAIVIPLSRFLLTQGLVIPLLFTAVTTLLFHVLVCWTLVFLFGLG 206

Query: 191 VVGAALALGISYWLNVILLGLYIFFSPSCKKTRAPFSRDAILSISEFFRLAVPSAVMTCL 250
             G A+A  +S+W   ++L  Y+ FS SC+KTR   SRD + SI +FF+  +PSA M CL
Sbjct: 207 CNGPAMATSVSFWFYAVILSCYVRFSSSCEKTRGFVSRDFVSSIKQFFQYGIPSAAMICL 266

Query: 251 EWWSYEVILLLSGLLPNPKVETSVLSICFSITYLHFFIPYGLAATVSTRVSNELGAGNPE 310
           EWW +E+++L SGLLPNPK+ETSVLSIC +I  LH+ I  G+AA VSTRVSN LGAGNP+
Sbjct: 267 EWWLFEILILCSGLLPNPKLETSVLSICLTIETLHYVISAGVAAAVSTRVSNNLGAGNPQ 326

Query: 311 AAKVAVRAVGILGIIESTIVSVTLFGCHNILGYAFTSDNQIAKHIASMWPLICLSIFIDS 370
            A+V+V A   L I+ES   S+ LF C NI+GYAF++  ++  ++A + PL+CLS  +D 
Sbjct: 327 VARVSVLAGLCLWIVESAFFSILLFTCRNIIGYAFSNSKEVLDYVADLTPLLCLSFILDG 386

Query: 371 FLAILTGVARGTGWQRLGAYVNLGSYYIVGIPMAAVLAFVAHLRVKGLWIGLVSGAVLQS 430
           F A+L GVARG+GWQ +GA+ N  SYY+VG P+   LAF   L  KGLW G+V G+ +Q+
Sbjct: 387 FTAVLNGVARGSGWQHIGAWNNTVSYYLVGAPVGIYLAFSRELNGKGLWCGVVVGSTVQA 446

Query: 431 FLFALITIFTNWHKQALKARGRIL 455
            + A++T   NW +QA KAR RI+
Sbjct: 447 TILAIVTASINWKEQAEKARKRIV 469

BLAST of Spg021595 vs. TAIR 10
Match: AT2G04090.1 (MATE efflux family protein )

HSP 1 Score: 446.4 bits (1147), Expect = 2.7e-125
Identity = 229/450 (50.89%), Postives = 314/450 (69.78%), Query Frame = 0

Query: 5   WRVYEKELKKVSFLAAPIVIAFVLQYLLQVEIVIIVGHLDDELLLSGVSIATSFARATGF 64
           W  +  ELK VS +AAP+    V QYLL V  V++ GH   EL LSGV++AT+FA  +GF
Sbjct: 24  WMNFTAELKNVSSMAAPMATVTVSQYLLPVISVMVAGHC-GELQLSGVTLATAFANVSGF 83

Query: 65  GLLLGMAGALETLCGQAYGAEQYQKLGVYTYSCTISLILVCFPISILWLFTDKLLISIGQ 124
           G++ G+ GALETLCGQAYGA+QY K+G YT+S  +S + +   ISILW + DKL +S+GQ
Sbjct: 84  GIMYGLVGALETLCGQAYGAKQYTKIGTYTFSAIVSNVPIVVLISILWFYMDKLFVSLGQ 143

Query: 125 DPSISYVARKYSVFLIPNLFAYAILQSLMRYLFTQSLIIPLLVCSFVTLSLHIPISWLLV 184
           DP IS VA  Y+V LIP L A A+ Q L R+L TQ L++PLL C+  TL  HIP+  +LV
Sbjct: 144 DPDISKVAGSYAVCLIPALLAQAVQQPLTRFLQTQGLVLPLLYCAITTLLFHIPVCLILV 203

Query: 185 FHFNLKVVGAALALGISYWLNVILLGLYIFFSPSCKKTRAPFSRDAILSISEFFRLAVPS 244
           + F L   GAALA+G+SYW NV++L LY+ FS +C+KTR   S D +LS+ +FF+  +PS
Sbjct: 204 YAFGLGSNGAALAIGLSYWFNVLILALYVRFSSACEKTRGFVSDDFVLSVKQFFQYGIPS 263

Query: 245 AVMTCLEWWSYEVILLLSGLLPNPKVETSVLSICFSITYLHFFIPYGLAATVSTRVSNEL 304
           A MT +EW  +E+++L SGLLPNPK+ETSVLSIC + + LH  IP G+ A  STR+SNEL
Sbjct: 264 AAMTTIEWSLFELLILSSGLLPNPKLETSVLSICLTTSSLHCVIPMGIGAAGSTRISNEL 323

Query: 305 GAGNPEAAKVAVRAVGILGIIESTIVSVTLFGCHNILGYAFTSDNQIAKHIASMWPLICL 364
           GAGNPE A++AV A   L  +E+TI S  LF C NI GYAF++  ++  ++  +  L+CL
Sbjct: 324 GAGNPEVARLAVFAGIFLWFLEATICSTLLFTCKNIFGYAFSNSKEVVDYVTELSSLLCL 383

Query: 365 SIFIDSFLAILTGVARGTGWQRLGAYVNLGSYYIVGIPMAAVLAFVAHLRVKGLWIGLVS 424
           S  +D F ++L GVARG+GWQ +GA+ N+ +YY++G P+   L F  H+  KGLWIG++ 
Sbjct: 384 SFMVDGFSSVLDGVARGSGWQNIGAWANVVAYYLLGAPVGFFLGFWGHMNGKGLWIGVIV 443

Query: 425 GAVLQSFLFALITIFTNWHKQALKARGRIL 455
           G+  Q  + A++T   +W +QA KAR RI+
Sbjct: 444 GSTAQGIILAIVTACLSWEEQAAKARERIV 472

BLAST of Spg021595 vs. TAIR 10
Match: AT2G04100.1 (MATE efflux family protein )

HSP 1 Score: 444.1 bits (1141), Expect = 1.3e-124
Identity = 230/451 (51.00%), Postives = 315/451 (69.84%), Query Frame = 0

Query: 5   WRV-YEKELKKVSFLAAPIVIAFVLQYLLQVEIVIIVGHLDDELLLSGVSIATSFARATG 64
           WR+ +  ELK +S +A P+    V QYLL V  V++ GH   EL LSGV++ATSF   +G
Sbjct: 23  WRMNFTAELKNLSRMALPMATVTVAQYLLPVISVMVAGH-RSELQLSGVALATSFTNVSG 82

Query: 65  FGLLLGMAGALETLCGQAYGAEQYQKLGVYTYSCTISLILVCFPISILWLFTDKLLISIG 124
           F ++ G+AGALETLCGQAYGA+QY K+G YT+S  +S + +   ISILW + DKL +S+G
Sbjct: 83  FSVMFGLAGALETLCGQAYGAKQYAKIGTYTFSAIVSNVPIVVLISILWFYMDKLFVSLG 142

Query: 125 QDPSISYVARKYSVFLIPNLFAYAILQSLMRYLFTQSLIIPLLVCSFVTLSLHIPISWLL 184
           QDP IS VA  Y+V LIP L A A+ Q L R+L TQ L++PLL C+  TL  HIP+  +L
Sbjct: 143 QDPDISKVAGSYAVCLIPALLAQAVQQPLTRFLQTQGLVLPLLYCAITTLLFHIPVCLIL 202

Query: 185 VFHFNLKVVGAALALGISYWLNVILLGLYIFFSPSCKKTRAPFSRDAILSISEFFRLAVP 244
           V+ F L   GAALA+G+SYW NV++L LY+ FS SC+KTR   S D +LS+ +FF+  +P
Sbjct: 203 VYAFGLGSNGAALAIGLSYWFNVLILALYVRFSSSCEKTRGFVSDDFVLSVKQFFQYGIP 262

Query: 245 SAVMTCLEWWSYEVILLLSGLLPNPKVETSVLSICFSITYLHFFIPYGLAATVSTRVSNE 304
           SA MT +EW  +E ++L SGLLPNPK+ETSVLSIC + + LH+ IP G+ A  S RVSNE
Sbjct: 263 SAAMTTIEWSLFEFLILSSGLLPNPKLETSVLSICLTTSSLHYVIPMGIGAAGSIRVSNE 322

Query: 305 LGAGNPEAAKVAVRAVGILGIIESTIVSVTLFGCHNILGYAFTSDNQIAKHIASMWPLIC 364
           LGAGNPE A++AV A   L  +E+TI S  LF C +I GYAF++  ++  ++  + PL+C
Sbjct: 323 LGAGNPEVARLAVFAGIFLWFLEATICSTLLFICRDIFGYAFSNSKEVVDYVTELSPLLC 382

Query: 365 LSIFIDSFLAILTGVARGTGWQRLGAYVNLGSYYIVGIPMAAVLAFVAHLRVKGLWIGLV 424
           +S  +D F A+L GVARG+GWQ +GA+ N+ +YY++G P+   L F  H+  KGLWIG+V
Sbjct: 383 ISFLVDGFSAVLGGVARGSGWQHIGAWANVVAYYLLGAPVGLFLGFWCHMNGKGLWIGVV 442

Query: 425 SGAVLQSFLFALITIFTNWHKQALKARGRIL 455
            G+  Q  + A++T   +W++QA KAR RI+
Sbjct: 443 VGSTAQGIILAIVTACMSWNEQAAKARQRIV 472

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023535495.14.1e-21684.50protein DETOXIFICATION 8-like isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_022973951.12.6e-21584.28protein DETOXIFICATION 8-like isoform X1 [Cucurbita maxima][more]
KAG6592362.11.0e-21483.84Protein DETOXIFICATION 14, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022925514.11.0e-21484.06protein DETOXIFICATION 8-like isoform X1 [Cucurbita moschata][more]
XP_022141868.18.5e-21485.81protein DETOXIFICATION 1-like isoform X2 [Momordica charantia][more]
Match NameE-valueIdentityDescription
F4HQ054.3e-12851.80Protein DETOXIFICATION 8 OS=Arabidopsis thaliana OX=3702 GN=DTX8 PE=3 SV=1[more]
Q9SIA44.8e-12752.48Protein DETOXIFICATION 3 OS=Arabidopsis thaliana OX=3702 GN=DTX3 PE=3 SV=1[more]
Q9SIA55.3e-12652.70Protein DETOXIFICATION 1 OS=Arabidopsis thaliana OX=3702 GN=DTX1 PE=2 SV=1[more]
Q9SIA13.8e-12450.89Protein DETOXIFICATION 5 OS=Arabidopsis thaliana OX=3702 GN=DTX5 PE=3 SV=2[more]
Q8RWF51.9e-12351.00Protein DETOXIFICATION 6 OS=Arabidopsis thaliana OX=3702 GN=DTX6 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1IA181.3e-21584.28Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111472575 PE=3 SV=1[more]
A0A6J1ECE14.9e-21584.06Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111432792 PE=3 SV=1[more]
A0A6J1CLS84.1e-21485.81Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111012127 PE=3 SV=1[more]
A0A6J1CK031.2e-20885.87Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111012127 PE=3 SV=1[more]
A0A6J1CL181.5e-20886.07Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111012127 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G66780.13.1e-12951.80MATE efflux family protein [more]
AT2G04050.13.4e-12852.48MATE efflux family protein [more]
AT2G04040.13.8e-12752.70MATE efflux family protein [more]
AT2G04090.12.7e-12550.89MATE efflux family protein [more]
AT2G04100.11.3e-12451.00MATE efflux family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002528Multi antimicrobial extrusion proteinTIGRFAMTIGR00797TIGR00797coord: 21..419
e-value: 6.8E-76
score: 253.7
IPR002528Multi antimicrobial extrusion proteinPFAMPF01554MatEcoord: 243..405
e-value: 1.2E-29
score: 103.1
coord: 21..182
e-value: 5.4E-30
score: 104.2
NoneNo IPR availablePANTHERPTHR11206:SF345PROTEIN DETOXIFICATIONcoord: 10..449
NoneNo IPR availablePANTHERPTHR11206MULTIDRUG RESISTANCE PROTEINcoord: 10..449
IPR045069Multidrug and toxic compound extrusion family, eukaryoticCDDcd13132MATE_eukaryoticcoord: 11..448
e-value: 4.69714E-165
score: 470.517

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg021595.1Spg021595.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1990961 xenobiotic detoxification by transmembrane export across the plasma membrane
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015297 antiporter activity
molecular_function GO:0042910 xenobiotic transmembrane transporter activity