Spg021484 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg021484 vs. NCBI nr
Match: XP_038890278.1 (non-lysosomal glucosylceramidase-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1743.0 bits (4513), Expect = 0.0e+00
Identity = 833/945 (88.15%), Postives = 884/945 (93.54%), Query Frame = 0

Query: 7   EEGNNGASSTKVDPAKLPSLTWQRRLDFTGKSPESFSFTLNDMWHLGSLGYRLWRHGKEE 66
           E+G NGASST VDP+K PSLTW+R+LDFTGKSPESFSFTL D WHLGSLGYRLWRHGKEE
Sbjct: 2   EKGGNGASSTTVDPSKPPSLTWKRKLDFTGKSPESFSFTLTDAWHLGSLGYRLWRHGKEE 61

Query: 67  AAKGRIPLFEFFSSQPITCHHGVSLGGIGAGSMGRSYRGEFQRFQMFFGPSEEEPVLANQ 126
            AKGRIP++EFFS QPITC+HGVSLGGIGAGS+GRSYRGEFQRFQMF+GP E+EPVLANQ
Sbjct: 62  TAKGRIPVYEFFSDQPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPCEDEPVLANQ 121

Query: 127 FSAFVSRPNGNKFSTVLCSAKPQKSKDGKQDGIESWDWNLSGENSTYHALFPRAWTIYDG 186
           FS FVSRPNGNKFS+VLC AKPQKSKDGKQDGIESWDWNLSG NSTYHALFPR+WTIYDG
Sbjct: 122 FSVFVSRPNGNKFSSVLCFAKPQKSKDGKQDGIESWDWNLSGANSTYHALFPRSWTIYDG 181

Query: 187 EPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNEGQTSAEVTLLFTWAVSAAKSIL 246
           EPDPDLKIVCRQISP IPHNYKESSFPVSVFTFNLSNEGQTSA+VTLLFTWA S      
Sbjct: 182 EPDPDLKIVCRQISPFIPHNYKESSFPVSVFTFNLSNEGQTSAQVTLLFTWANSVGGKSG 241

Query: 247 CSFHGFILIQWSEDGAHGVLLHHKTANGRPTVTYAIAAEATDDVHVSVCPCFVISGDSEG 306
            + H F     +EDGAHGVLLHHKT  GRPTVTYAIAAE TDDVH+SVCPCFVISGDSEG
Sbjct: 242 FTGHHFNSKMGAEDGAHGVLLHHKTTKGRPTVTYAIAAEETDDVHISVCPCFVISGDSEG 301

Query: 307 ISAKDMWQEIKNHGSFEKLGSVGAYEGSKPGSSIGAAIAATVTIPSASARTVTFSLAWDC 366
           ISAKDMWQEIKNHGSF+KLGSVG YEGSKPGSSIGAA+AAT+TIPS+ ARTVTFSLAWDC
Sbjct: 302 ISAKDMWQEIKNHGSFDKLGSVGQYEGSKPGSSIGAAVAATLTIPSSVARTVTFSLAWDC 361

Query: 367 PEVKFDGKTYHRQYTKFYGTLGDAAKVIARDGILEHGKWERQIEEWQKPIVEDKRLPEWY 426
           PEVKFDGKTYHR+Y+KFYGTLGDAAK+IARD ILEHGKWE QIE WQ+PI+EDKRLPEWY
Sbjct: 362 PEVKFDGKTYHRRYSKFYGTLGDAAKIIARDAILEHGKWEAQIEAWQRPIIEDKRLPEWY 421

Query: 427 PVTLFNELYFLNSGGTIWTDGLPPLQNLSTISDQKYFLQRSKSEPN-GVPNGGHRYDVAI 486
           PVTL NELYFLN+GGTIWTDGLPPLQNLSTI+ QKYFL+RSKSEPN G  NG HR DVA+
Sbjct: 422 PVTLLNELYFLNAGGTIWTDGLPPLQNLSTITHQKYFLERSKSEPNGGALNGDHRKDVAV 481

Query: 487 DILERMSQIFDQSHGGAGPSNAALGTRLLLPGEENVGQLLLVEGSQYLMWNTYDVHFYSS 546
           DILERMSQIFDQ+HGGAGPSNAALGTRLL PGEENVG LLLVEGSQYLMWNTYDVHFYSS
Sbjct: 482 DILERMSQIFDQTHGGAGPSNAALGTRLLHPGEENVGNLLLVEGSQYLMWNTYDVHFYSS 541

Query: 547 FALIMLFPKLELSIQRDFAAAVLMHDPRKARIMSDGNWVPRKVIGAVPHDIGFNDPWFEV 606
           FAL+MLFPKLELSIQRDFAAAVLMHDPRKA+IMSDGNWVPRKV+GAVPHD+GFNDPWFEV
Sbjct: 542 FALVMLFPKLELSIQRDFAAAVLMHDPRKAKIMSDGNWVPRKVLGAVPHDVGFNDPWFEV 601

Query: 607 NAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMEQFDKDKDGMV 666
           NAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFA+SVWPSVYVALAFMEQFDKDKDGM+
Sbjct: 602 NAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAQSVWPSVYVALAFMEQFDKDKDGMI 661

Query: 667 ENEGFPDQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDDEAAADYFWVKYQKARDVY 726
           ENEGFPDQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDDEAAA YFW KYQKA+ VY
Sbjct: 662 ENEGFPDQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDDEAAAHYFWTKYQKAKSVY 721

Query: 727 GTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPITDEEKIRSALEKIYNFNVM 786
            TLWNGSYFNYDNS GPWSSSIQADQLAGQWYARACGLCP+ DEEKIRSALEKIYNFNVM
Sbjct: 722 DTLWNGSYFNYDNSKGPWSSSIQADQLAGQWYARACGLCPVADEEKIRSALEKIYNFNVM 781

Query: 787 KVKGGSRGAVNGMFPDGRVDTSMLQPKEIWAGVTYSLAASMIQEGMVETGFLTAMGVHQA 846
           KVKGG+RGAVNGMFPDGRVD S+LQPKEIW GVTYS+AASMIQEG+VETGF TAMG+HQA
Sbjct: 782 KVKGGTRGAVNGMFPDGRVDKSILQPKEIWPGVTYSVAASMIQEGLVETGFQTAMGIHQA 841

Query: 847 AWAQDGLGYSFQTPEAWDVNDKYRSLGYMRPLAIWAMQWALSKPTLVKKKHKIPTKVLSE 906
           AWAQDGLGYSFQTPEAWDV+D+YRS+GYMRPLAIWAMQWA+SKPT      KIPTKVLSE
Sbjct: 842 AWAQDGLGYSFQTPEAWDVDDRYRSIGYMRPLAIWAMQWAISKPT------KIPTKVLSE 901

Query: 907 IEESSFAAQHAAFLKVASLLKLPSKEAAHRSLVEVAYDFICKRSA 951
           +EES+FA QHAAFLKVASLLKLPS++AAHRSLVE AYDFICKRSA
Sbjct: 902 MEESAFATQHAAFLKVASLLKLPSEDAAHRSLVEAAYDFICKRSA 940

BLAST of Spg021484 vs. NCBI nr
Match: XP_023524725.1 (non-lysosomal glucosylceramidase-like isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1724.5 bits (4465), Expect = 0.0e+00
Identity = 835/959 (87.07%), Postives = 886/959 (92.39%), Query Frame = 0

Query: 1   MENGNKEEGNNGASSTKVDPAKLPSLTWQRRLDFTGKSPESFSFTLNDMWHLGSLGYRLW 60
           MENG KEE +NGASST VDPAKLPSLTWQR+LDFTGKSPESFSFTLND WHLG LGYRLW
Sbjct: 1   MENGCKEEADNGASSTMVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRLW 60

Query: 61  RHGKEEAAKGRIPLFEFFSSQPITCHHGVSLGGIGAGSMGRSYRGEFQRFQMFFGPSEEE 120
           RHGKEEAAKGRIPL++FF+S PITC+HGVSLGGIGAGS+GRSYRGEFQRFQMF+GPSE+ 
Sbjct: 61  RHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSEDV 120

Query: 121 PVLANQFSAFVSRPNGNKFSTVLCSAKPQKSKDGKQDGIESWDWNLSGENSTYHALFPRA 180
           PVLANQFSAFVSRPNGNKFSTVLCSA+PQKSKDGK DGIE+WDWNLSGE STYHALFPRA
Sbjct: 121 PVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPRA 180

Query: 181 WTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNEGQTSAEVTLLFTWAVS 240
           WTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSN+G TSAEVTLLFTWA S
Sbjct: 181 WTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWANS 240

Query: 241 AAKSILCSFHGF--------ILIQWSEDGAHGVLLHHKTANGRPTVTYAIAAEATDDVHV 300
              +   + H F          +  +EDGAHGVLLHHKTANGRPTVTYAI AEATDDVHV
Sbjct: 241 VGGNSGFTGHHFNSKIGVKAEFVSSAEDGAHGVLLHHKTANGRPTVTYAITAEATDDVHV 300

Query: 301 SVCPCFVISGDSEGISAKDMWQEIKNHGSFEKLGSVGAYEGSKPGSSIGAAIAATVTIPS 360
           SVCP FVISGDSEGISAKDMWQE+KNHGSF++LGS G +EGSKPGSSIGAAIAA VTIPS
Sbjct: 301 SVCPRFVISGDSEGISAKDMWQEVKNHGSFDELGSSGPHEGSKPGSSIGAAIAAKVTIPS 360

Query: 361 ASARTVTFSLAWDCPEVKFDGKTYHRQYTKFYGTLGDAAKVIARDGILEHGKWERQIEEW 420
           +S RTVTFSLAWDCPEVKFDGKTYHR+YT FYGTLG+AAK IARD ILEHGKWE QIE W
Sbjct: 361 SSTRTVTFSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEAW 420

Query: 421 QKPIVEDKRLPEWYPVTLFNELYFLNSGGTIWTDGLPPLQNLSTISDQKYFLQRSKSEPN 480
           Q+PIVEDKRLPEWYPVTLFNELYFLNSGGT+WTDGLPPLQNLSTIS QK FL+R+KSE N
Sbjct: 421 QRPIVEDKRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSESN 480

Query: 481 GVPNGGHRYDVAIDILERMSQIFDQSHGGAGPSNAALGTRLLLPGEENVGQLLLVEGSQY 540
           G PNG HR DVA++ILERMSQIFDQ+HGGAGPSNAALGTRLL   +ENVGQLLLVEGS+Y
Sbjct: 481 GAPNGDHRLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSEY 540

Query: 541 LMWNTYDVHFYSSFALIMLFPKLELSIQRDFAAAVLMHDPRKARIMSDGNWVPRKVIGAV 600
           LMWNT+DVHFYSSFALIMLFPKLEL IQRDFAAAVLMHDPRKA+IMSDG + PRKVIGAV
Sbjct: 541 LMWNTFDVHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGAV 600

Query: 601 PHDIGFNDPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALA 660
           PHDIGFNDPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALA
Sbjct: 601 PHDIGFNDPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALA 660

Query: 661 FMEQFDKDKDGMVENEGFPDQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDDEAAAD 720
           FM+QFDKDKDGM+ENEGFPDQTYD WTVKGVSAYCGGLWVAALQAASALA EVDDEAAA 
Sbjct: 661 FMDQFDKDKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAAH 720

Query: 721 YFWVKYQKARDVYGTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPITDEEKI 780
           YFW+KYQKAR VY TLWNGSYFNYDNSGGPWSSSIQADQLAG+WYARACGLCPI DEEKI
Sbjct: 721 YFWIKYQKARGVYDTLWNGSYFNYDNSGGPWSSSIQADQLAGKWYARACGLCPIVDEEKI 780

Query: 781 RSALEKIYNFNVMKVKGGSRGAVNGMFPDGRVDTSMLQPKEIWAGVTYSLAASMIQEGMV 840
           RS LEKI+NFNVMKVKGG+RGAVNGMFPDGRVD S+LQPKEIWAGVTYS+AASMIQEGMV
Sbjct: 781 RSTLEKIFNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGMV 840

Query: 841 ETGFLTAMGVHQAAWAQDGLGYSFQTPEAWDVNDKYRSLGYMRPLAIWAMQWALSKPTLV 900
           ETGF TAMG+HQAAW QDGLGYSFQTPEAWD++DKYRSLGYMRPLAIWAMQWA+SKPTL+
Sbjct: 841 ETGFQTAMGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTLL 900

Query: 901 KKKHKIPTKVLSEIEESS-FAAQHAAFLKVASLLKLPSKEAAHRSLVEVAYDFICKRSA 951
            KKHK+P K LSE EES  FA QHAAFLKVASLLKLPS+EA  RS+VEVAYDFICKRSA
Sbjct: 901 -KKHKVPRKELSESEESCLFATQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA 958

BLAST of Spg021484 vs. NCBI nr
Match: XP_023524726.1 (non-lysosomal glucosylceramidase-like isoform X4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1723.4 bits (4462), Expect = 0.0e+00
Identity = 835/952 (87.71%), Postives = 885/952 (92.96%), Query Frame = 0

Query: 1   MENGNKEEGNNGASSTK-VDPAKLPSLTWQRRLDFTGKSPESFSFTLNDMWHLGSLGYRL 60
           MENG KEE +NGASST  VDPAKLPSLTWQR+LDFTGKSPESFSFTLND WHLG LGYRL
Sbjct: 1   MENGCKEEADNGASSTMVVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRL 60

Query: 61  WRHGKEEAAKGRIPLFEFFSSQPITCHHGVSLGGIGAGSMGRSYRGEFQRFQMFFGPSEE 120
           WRHGKEEAAKGRIPL++FF+S PITC+HGVSLGGIGAGS+GRSYRGEFQRFQMF+GPSE+
Sbjct: 61  WRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSED 120

Query: 121 EPVLANQFSAFVSRPNGNKFSTVLCSAKPQKSKDGKQDGIESWDWNLSGENSTYHALFPR 180
            PVLANQFSAFVSRPNGNKFSTVLCSA+PQKSKDGK DGIE+WDWNLSGE STYHALFPR
Sbjct: 121 VPVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPR 180

Query: 181 AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNEGQTSAEVTLLFTWAV 240
           AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSN+G TSAEVTLLFTWA 
Sbjct: 181 AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWAN 240

Query: 241 SAAKSILCSFHGFILIQWSEDGAHGVLLHHKTANGRPTVTYAIAAEATDDVHVSVCPCFV 300
           S   +   + H F     +EDGAHGVLLHHKTANGRPTVTYAI AEATDDVHVSVCP FV
Sbjct: 241 SVGGNSGFTGHHFNSKIGAEDGAHGVLLHHKTANGRPTVTYAITAEATDDVHVSVCPRFV 300

Query: 301 ISGDSEGISAKDMWQEIKNHGSFEKLGSVGAYEGSKPGSSIGAAIAATVTIPSASARTVT 360
           ISGDSEGISAKDMWQE+KNHGSF++LGS G +EGSKPGSSIGAAIAA VTIPS+S RTVT
Sbjct: 301 ISGDSEGISAKDMWQEVKNHGSFDELGSSGPHEGSKPGSSIGAAIAAKVTIPSSSTRTVT 360

Query: 361 FSLAWDCPEVKFDGKTYHRQYTKFYGTLGDAAKVIARDGILEHGKWERQIEEWQKPIVED 420
           FSLAWDCPEVKFDGKTYHR+YT FYGTLG+AAK IARD ILEHGKWE QIE WQ+PIVED
Sbjct: 361 FSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEAWQRPIVED 420

Query: 421 KRLPEWYPVTLFNELYFLNSGGTIWTDGLPPLQNLSTISDQKYFLQRSKSEPNGVPNGGH 480
           KRLPEWYPVTLFNELYFLNSGGT+WTDGLPPLQNLSTIS QK FL+R+KSE NG PNG H
Sbjct: 421 KRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSESNGAPNGDH 480

Query: 481 RYDVAIDILERMSQIFDQSHGGAGPSNAALGTRLLLPGEENVGQLLLVEGSQYLMWNTYD 540
           R DVA++ILERMSQIFDQ+HGGAGPSNAALGTRLL   +ENVGQLLLVEGS+YLMWNT+D
Sbjct: 481 RLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSEYLMWNTFD 540

Query: 541 VHFYSSFALIMLFPKLELSIQRDFAAAVLMHDPRKARIMSDGNWVPRKVIGAVPHDIGFN 600
           VHFYSSFALIMLFPKLEL IQRDFAAAVLMHDPRKA+IMSDG + PRKVIGAVPHDIGFN
Sbjct: 541 VHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGAVPHDIGFN 600

Query: 601 DPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMEQFDK 660
           DPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFM+QFDK
Sbjct: 601 DPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMDQFDK 660

Query: 661 DKDGMVENEGFPDQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDDEAAADYFWVKYQ 720
           DKDGM+ENEGFPDQTYD WTVKGVSAYCGGLWVAALQAASALA EVDDEAAA YFW+KYQ
Sbjct: 661 DKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAAHYFWIKYQ 720

Query: 721 KARDVYGTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPITDEEKIRSALEKI 780
           KAR VY TLWNGSYFNYDNSGGPWSSSIQADQLAG+WYARACGLCPI DEEKIRS LEKI
Sbjct: 721 KARGVYDTLWNGSYFNYDNSGGPWSSSIQADQLAGKWYARACGLCPIVDEEKIRSTLEKI 780

Query: 781 YNFNVMKVKGGSRGAVNGMFPDGRVDTSMLQPKEIWAGVTYSLAASMIQEGMVETGFLTA 840
           +NFNVMKVKGG+RGAVNGMFPDGRVD S+LQPKEIWAGVTYS+AASMIQEGMVETGF TA
Sbjct: 781 FNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGMVETGFQTA 840

Query: 841 MGVHQAAWAQDGLGYSFQTPEAWDVNDKYRSLGYMRPLAIWAMQWALSKPTLVKKKHKIP 900
           MG+HQAAW QDGLGYSFQTPEAWD++DKYRSLGYMRPLAIWAMQWA+SKPTL+ KKHK+P
Sbjct: 841 MGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTLL-KKHKVP 900

Query: 901 TKVLSEIEESS-FAAQHAAFLKVASLLKLPSKEAAHRSLVEVAYDFICKRSA 951
            K LSE EES  FA QHAAFLKVASLLKLPS+EA  RS+VEVAYDFICKRSA
Sbjct: 901 RKELSESEESCLFATQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA 951

BLAST of Spg021484 vs. NCBI nr
Match: XP_022948946.1 (non-lysosomal glucosylceramidase-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1723.0 bits (4461), Expect = 0.0e+00
Identity = 833/959 (86.86%), Postives = 886/959 (92.39%), Query Frame = 0

Query: 1   MENGNKEEGNNGASSTKVDPAKLPSLTWQRRLDFTGKSPESFSFTLNDMWHLGSLGYRLW 60
           MENG KE+ +NGASST VDPAKLPSLTWQR+LDFTGKSPESFSFTLND WHLG LGYRLW
Sbjct: 1   MENGCKEDADNGASSTMVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRLW 60

Query: 61  RHGKEEAAKGRIPLFEFFSSQPITCHHGVSLGGIGAGSMGRSYRGEFQRFQMFFGPSEEE 120
           RHGKEEAAKGRIPL++FF+S PITC+HGVSLGGIGAGS+GRSYRGEFQRFQMF+GPSE+ 
Sbjct: 61  RHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSEDV 120

Query: 121 PVLANQFSAFVSRPNGNKFSTVLCSAKPQKSKDGKQDGIESWDWNLSGENSTYHALFPRA 180
           PVLANQFSAFVSRPNGNKFSTVLCSA+PQKSKDGK DGIE+WDWNLSGE STYHALFPRA
Sbjct: 121 PVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPRA 180

Query: 181 WTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNEGQTSAEVTLLFTWAVS 240
           WTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSN+G TSAEVTLLFTWA S
Sbjct: 181 WTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWANS 240

Query: 241 AAKSILCSFHGF--------ILIQWSEDGAHGVLLHHKTANGRPTVTYAIAAEATDDVHV 300
              +   + H F          +  +EDGAHGVLLHHKTANGRPTVTYAI AEATDDVHV
Sbjct: 241 VGGNSGFTGHHFNSKIGVKAEFVSSAEDGAHGVLLHHKTANGRPTVTYAITAEATDDVHV 300

Query: 301 SVCPCFVISGDSEGISAKDMWQEIKNHGSFEKLGSVGAYEGSKPGSSIGAAIAATVTIPS 360
           SVCP FVISGDSEGISAKDMWQE+K HGSF++LGS G +EGSKPGSSIGAAIAA V+IPS
Sbjct: 301 SVCPRFVISGDSEGISAKDMWQEVKKHGSFDELGSSGPHEGSKPGSSIGAAIAAKVSIPS 360

Query: 361 ASARTVTFSLAWDCPEVKFDGKTYHRQYTKFYGTLGDAAKVIARDGILEHGKWERQIEEW 420
           +S RTVTFSLAWDCPEVKFDGKTYHR+YT FYGTLG+AAK IARD ILEHGKWE QIE W
Sbjct: 361 SSTRTVTFSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEAW 420

Query: 421 QKPIVEDKRLPEWYPVTLFNELYFLNSGGTIWTDGLPPLQNLSTISDQKYFLQRSKSEPN 480
           Q+PIVEDKRLPEWYPVTLFNELYFLNSGGT+WTDGLPPLQNLSTIS QK FL+R+KSEPN
Sbjct: 421 QRPIVEDKRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSEPN 480

Query: 481 GVPNGGHRYDVAIDILERMSQIFDQSHGGAGPSNAALGTRLLLPGEENVGQLLLVEGSQY 540
           G PNG HR DVA++ILERMSQIFDQ+HGGAGPSNAALGTRLL   +ENVGQLLLVEGS+Y
Sbjct: 481 GAPNGDHRLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSEY 540

Query: 541 LMWNTYDVHFYSSFALIMLFPKLELSIQRDFAAAVLMHDPRKARIMSDGNWVPRKVIGAV 600
           LMWNT+DVHFYSSFALIMLFPKLEL IQRDFAAAVLMHDPRKA+IMSDG + PRKVIGAV
Sbjct: 541 LMWNTFDVHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGAV 600

Query: 601 PHDIGFNDPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALA 660
           PHDIGFNDPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALA
Sbjct: 601 PHDIGFNDPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALA 660

Query: 661 FMEQFDKDKDGMVENEGFPDQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDDEAAAD 720
           FM+QFDKDKDGM+ENEGFPDQTYD WTVKGVSAYCGGLWVAALQAASALA EVDDEAAA 
Sbjct: 661 FMDQFDKDKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAAH 720

Query: 721 YFWVKYQKARDVYGTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPITDEEKI 780
           YFW+KYQKAR VY TLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPI DEEKI
Sbjct: 721 YFWIKYQKARGVYDTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPIVDEEKI 780

Query: 781 RSALEKIYNFNVMKVKGGSRGAVNGMFPDGRVDTSMLQPKEIWAGVTYSLAASMIQEGMV 840
           RS LEKI+NFNVMKVKGG+RGAVNGMFPDGRVD S+LQPKEIWAGVTYS+AASMIQEGMV
Sbjct: 781 RSTLEKIFNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGMV 840

Query: 841 ETGFLTAMGVHQAAWAQDGLGYSFQTPEAWDVNDKYRSLGYMRPLAIWAMQWALSKPTLV 900
           ETGF TAMG+HQAAW QDGLGYSFQTPEAWD++DKYRSLGYMRPLAIWAMQWA+SKPTL+
Sbjct: 841 ETGFQTAMGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTLL 900

Query: 901 KKKHKIPTKVLSEIEESS-FAAQHAAFLKVASLLKLPSKEAAHRSLVEVAYDFICKRSA 951
            KKHK+P K LSE EES  F+ QHAAFLKVASLLKLPS+EA  RS+VEVAYDFICKRSA
Sbjct: 901 -KKHKVPRKELSESEESCLFSTQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA 958

BLAST of Spg021484 vs. NCBI nr
Match: XP_022948947.1 (non-lysosomal glucosylceramidase-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 1721.8 bits (4458), Expect = 0.0e+00
Identity = 833/952 (87.50%), Postives = 885/952 (92.96%), Query Frame = 0

Query: 1   MENGNKEEGNNGASSTK-VDPAKLPSLTWQRRLDFTGKSPESFSFTLNDMWHLGSLGYRL 60
           MENG KE+ +NGASST  VDPAKLPSLTWQR+LDFTGKSPESFSFTLND WHLG LGYRL
Sbjct: 1   MENGCKEDADNGASSTMVVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRL 60

Query: 61  WRHGKEEAAKGRIPLFEFFSSQPITCHHGVSLGGIGAGSMGRSYRGEFQRFQMFFGPSEE 120
           WRHGKEEAAKGRIPL++FF+S PITC+HGVSLGGIGAGS+GRSYRGEFQRFQMF+GPSE+
Sbjct: 61  WRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSED 120

Query: 121 EPVLANQFSAFVSRPNGNKFSTVLCSAKPQKSKDGKQDGIESWDWNLSGENSTYHALFPR 180
            PVLANQFSAFVSRPNGNKFSTVLCSA+PQKSKDGK DGIE+WDWNLSGE STYHALFPR
Sbjct: 121 VPVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPR 180

Query: 181 AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNEGQTSAEVTLLFTWAV 240
           AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSN+G TSAEVTLLFTWA 
Sbjct: 181 AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWAN 240

Query: 241 SAAKSILCSFHGFILIQWSEDGAHGVLLHHKTANGRPTVTYAIAAEATDDVHVSVCPCFV 300
           S   +   + H F     +EDGAHGVLLHHKTANGRPTVTYAI AEATDDVHVSVCP FV
Sbjct: 241 SVGGNSGFTGHHFNSKIGAEDGAHGVLLHHKTANGRPTVTYAITAEATDDVHVSVCPRFV 300

Query: 301 ISGDSEGISAKDMWQEIKNHGSFEKLGSVGAYEGSKPGSSIGAAIAATVTIPSASARTVT 360
           ISGDSEGISAKDMWQE+K HGSF++LGS G +EGSKPGSSIGAAIAA V+IPS+S RTVT
Sbjct: 301 ISGDSEGISAKDMWQEVKKHGSFDELGSSGPHEGSKPGSSIGAAIAAKVSIPSSSTRTVT 360

Query: 361 FSLAWDCPEVKFDGKTYHRQYTKFYGTLGDAAKVIARDGILEHGKWERQIEEWQKPIVED 420
           FSLAWDCPEVKFDGKTYHR+YT FYGTLG+AAK IARD ILEHGKWE QIE WQ+PIVED
Sbjct: 361 FSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEAWQRPIVED 420

Query: 421 KRLPEWYPVTLFNELYFLNSGGTIWTDGLPPLQNLSTISDQKYFLQRSKSEPNGVPNGGH 480
           KRLPEWYPVTLFNELYFLNSGGT+WTDGLPPLQNLSTIS QK FL+R+KSEPNG PNG H
Sbjct: 421 KRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSEPNGAPNGDH 480

Query: 481 RYDVAIDILERMSQIFDQSHGGAGPSNAALGTRLLLPGEENVGQLLLVEGSQYLMWNTYD 540
           R DVA++ILERMSQIFDQ+HGGAGPSNAALGTRLL   +ENVGQLLLVEGS+YLMWNT+D
Sbjct: 481 RLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSEYLMWNTFD 540

Query: 541 VHFYSSFALIMLFPKLELSIQRDFAAAVLMHDPRKARIMSDGNWVPRKVIGAVPHDIGFN 600
           VHFYSSFALIMLFPKLEL IQRDFAAAVLMHDPRKA+IMSDG + PRKVIGAVPHDIGFN
Sbjct: 541 VHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGAVPHDIGFN 600

Query: 601 DPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMEQFDK 660
           DPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFM+QFDK
Sbjct: 601 DPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMDQFDK 660

Query: 661 DKDGMVENEGFPDQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDDEAAADYFWVKYQ 720
           DKDGM+ENEGFPDQTYD WTVKGVSAYCGGLWVAALQAASALA EVDDEAAA YFW+KYQ
Sbjct: 661 DKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAAHYFWIKYQ 720

Query: 721 KARDVYGTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPITDEEKIRSALEKI 780
           KAR VY TLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPI DEEKIRS LEKI
Sbjct: 721 KARGVYDTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPIVDEEKIRSTLEKI 780

Query: 781 YNFNVMKVKGGSRGAVNGMFPDGRVDTSMLQPKEIWAGVTYSLAASMIQEGMVETGFLTA 840
           +NFNVMKVKGG+RGAVNGMFPDGRVD S+LQPKEIWAGVTYS+AASMIQEGMVETGF TA
Sbjct: 781 FNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGMVETGFQTA 840

Query: 841 MGVHQAAWAQDGLGYSFQTPEAWDVNDKYRSLGYMRPLAIWAMQWALSKPTLVKKKHKIP 900
           MG+HQAAW QDGLGYSFQTPEAWD++DKYRSLGYMRPLAIWAMQWA+SKPTL+ KKHK+P
Sbjct: 841 MGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTLL-KKHKVP 900

Query: 901 TKVLSEIEESS-FAAQHAAFLKVASLLKLPSKEAAHRSLVEVAYDFICKRSA 951
            K LSE EES  F+ QHAAFLKVASLLKLPS+EA  RS+VEVAYDFICKRSA
Sbjct: 901 RKELSESEESCLFSTQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA 951

BLAST of Spg021484 vs. ExPASy Swiss-Prot
Match: Q9HCG7 (Non-lysosomal glucosylceramidase OS=Homo sapiens OX=9606 GN=GBA2 PE=1 SV=2)

HSP 1 Score: 564.3 bits (1453), Expect = 2.6e-159
Identity = 325/858 (37.88%), Postives = 460/858 (53.61%), Query Frame = 0

Query: 47  NDMWHLG-SLGYRLWRHGKEEAAKGRIPLFEFFSSQPITCHHGVSLGGIGAGSMGRSYRG 106
           N + H+G  L Y  W + K    K + P  +  +S P+   +G  LGGIG G++ R +RG
Sbjct: 110 NMIKHIGMGLRYLQWWYRKTHVEK-KTPFIDMINSVPLRQIYGCPLGGIGGGTITRGWRG 169

Query: 107 EFQRFQMFFGPSEEEPVLANQFSAFVSRPNGNKFSTVLCSAKPQKSKDGKQDGIESWDWN 166
           +F R+Q+  G  +   V+A+QF+  + R     +  VL   +P          + SW+W 
Sbjct: 170 QFCRWQLNPGMYQHRTVIADQFTVCLRREGQTVYQQVLSLERPSV--------LRSWNWG 229

Query: 167 LSGENSTYHALFPRAWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNEG 226
           L G  + YHAL+PRAWT+Y   P  ++ + CRQI+PI+PH+Y++SS PV VF +++ NEG
Sbjct: 230 LCGYFAFYHALYPRAWTVYQ-LPGQNVTLTCRQITPILPHDYQDSSLPVGVFVWDVENEG 289

Query: 227 QTSAEVTLLFTWAVSAAKSILCS---FHGFILIQWSEDGAHGVLLHHKTANGRPTVTYAI 286
             + +V+++F+               ++    ++ S +   G+LLHH T       T A+
Sbjct: 290 DEALDVSIMFSMRNGLGGGDDAPGGLWNEPFCLERSGETVRGLLLHHPTLPN--PYTMAV 349

Query: 287 AAEATDDVHVSVCPCFVISGDSEGISAKDMWQEIKNHGSFEKLGSVGAYEGSKPGSSIGA 346
           AA  T    V+    F    DS G   + +WQ++   G  +     G    ++ G  I  
Sbjct: 350 AARVTAATTVTHITAF--DPDSTG---QQVWQDLLQDGQLD--SPTGQSTPTQKGVGIAG 409

Query: 347 AIAATVTIPSASARTVTFSLAWDCPEVKF--DGKTYHRQYTKFYGTLGDAAKVIARDGIL 406
           A+  +  +       + FSLAWD P + F   G+ ++R+YT+F+G  GDAA  ++   + 
Sbjct: 410 AVCVSSKLRPRGQCRLEFSLAWDMPRIMFGAKGQVHYRRYTRFFGQDGDAAPALSHYALC 469

Query: 407 EHGKWERQIEEWQKPIVEDKRLPEWYPVTLFNELYFLNSGGTIWTDGLPPLQNLSTISDQ 466
            + +WE +I  WQ P+++D+ LP WY   LFNELYFL  GGT+W                
Sbjct: 470 RYAEWEERISAWQSPVLDDRSLPAWYKSALFNELYFLADGGTVW---------------- 529

Query: 467 KYFLQRSKSEPNGVPNGGHRYDVAIDILERMSQIFDQSHGGAGPSNAALGTRLLLPGEEN 526
                                   +++LE            + P         L P   +
Sbjct: 530 ------------------------LEVLE-----------DSLPEELGRNMCHLRPTLRD 589

Query: 527 VGQLLLVEGSQYLMWNTYDVHFYSSFALIMLFPKLELSIQRDFAAAVLMHDPRKARIMSD 586
            G+   +EG +Y M+NTYDVHFY+SFALIML+PKLELS+Q D A A L  D  + R +  
Sbjct: 590 YGRFGYLEGQEYRMYNTYDVHFYASFALIMLWPKLELSLQYDMALATLREDLTRRRYLMS 649

Query: 587 GNWVPRKVIGAVPHDIGFND--PWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKN 646
           G   P K    +PHDIG  D  PW  VNAY + +   WKDL  KFVLQVYRD   TGD+N
Sbjct: 650 GVMAPVKRRNVIPHDIGDPDDEPWLRVNAYLIHDTADWKDLNLKFVLQVYRDYYLTGDQN 709

Query: 647 FAKSVWPSVYVALAFME---QFDKDKDGMVENEGFPDQTYDTWTVKGVSAYCGGLWVAAL 706
           F K +WP   V LA ME   +FDKD DG++EN G+ DQTYD W   G SAYCGGLW+AA+
Sbjct: 710 FLKDMWP---VCLAVMESEMKFDKDHDGLIENGGYADQTYDGWVTTGPSAYCGGLWLAAV 769

Query: 707 QAASALASEVDDEAAADYFWVKYQKARDVY-GTLWNGSYFNYDNSGGPWSSSIQADQLAG 766
                +A+    +   D F     + ++ Y   LWNG Y+NYD+S  P S S+ +DQ AG
Sbjct: 770 AVMVQMAALCGAQDIQDKFSSILSRGQEAYERLLWNGRYYNYDSSSRPQSRSVMSDQCAG 829

Query: 767 QWYARACGL----CPITDEEKIRSALEKIYNFNVMKVKGGSRGAVNGMFPDGRVDTSMLQ 826
           QW+ +ACGL      +   + +  AL+ I+  NV    GG+ GAVNGM P G  D S +Q
Sbjct: 830 QWFLKACGLGEGDTEVFPTQHVVRALQTIFELNVQAFAGGAMGAVNGMQPHGVPDKSSVQ 889

Query: 827 PKEIWAGVTYSLAASMIQEGMVETGFLTAMGVHQAAWAQDGLGYSFQTPEAWDVNDKYRS 886
             E+W GV Y LAA+MIQEG+   GF TA G ++  W  + LG +FQTPEA+     +RS
Sbjct: 890 SDEVWVGVVYGLAATMIQEGLTWEGFQTAEGCYRTVW--ERLGLAFQTPEAYCQQRVFRS 892

Query: 887 LGYMRPLAIWAMQWALSK 889
           L YMRPL+IWAMQ AL +
Sbjct: 950 LAYMRPLSIWAMQLALQQ 892

BLAST of Spg021484 vs. ExPASy Swiss-Prot
Match: Q69ZF3 (Non-lysosomal glucosylceramidase OS=Mus musculus OX=10090 GN=Gba2 PE=1 SV=2)

HSP 1 Score: 560.1 bits (1442), Expect = 4.9e-158
Identity = 328/870 (37.70%), Postives = 465/870 (53.45%), Query Frame = 0

Query: 51  HLG-SLGYRLWRHGKEEAAKGRIPLFEFFSSQPITCHHGVSLGGIGAGSMGRSYRGEFQR 110
           HLG  L Y  W + K    K + P  +  +S P+   +G  LGGIG G++ R +RG+F R
Sbjct: 105 HLGMGLRYLKWWYRKTHVEK-KTPFIDMLNSLPLRQIYGCPLGGIGGGTITRGWRGQFCR 164

Query: 111 FQMFFGPSEEEPVLANQFSAFVSRPNGNKFSTVLCSAKPQKSKDGKQDGIESWDWNLSGE 170
           +Q+  G  + + V+A+QF   + R     +  VL    P        + + SW+W L G 
Sbjct: 165 WQLNPGMYQHQTVIADQFIVCLRRDGRTVYQQVLSLELP--------NVLRSWNWGLCGY 224

Query: 171 NSTYHALFPRAWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNEGQTSA 230
            + YHAL+PRAWT+Y   P  ++ + CRQ++PI+PH+Y++SS PV VF +++ NEG  + 
Sbjct: 225 FAFYHALYPRAWTVYQ-LPGQNVTLTCRQVTPILPHDYQDSSLPVGVFVWDVENEGDETL 284

Query: 231 EVTLLFTW------AVSAAKSILCSFHGFILIQWSEDGAHGVLLHHKTANGRPTVTYAIA 290
           +V++ F+          AA S+   ++    ++       G+LLHH T       T A+A
Sbjct: 285 DVSITFSMRNGLGGEDDAAGSL---WNEPFRLEQGGTTVQGLLLHHPTPPN--PYTMAVA 344

Query: 291 AEATDDVHVSVCPCFVISGDSEGISAKDMWQEIKNHGSFEKLGSVGAYEGSKPGSSIGAA 350
           A  T D  V+    F  +G     + + +WQ++   G  +     G    ++ G  I  A
Sbjct: 345 ARCTADTTVTHTTAFDPNG-----TGQQVWQDLLQDGQLD--SPAGQSTPTQKGEGIAGA 404

Query: 351 IAATVTIPSASARTVTFSLAWDCPEVKFDGKT--YHRQYTKFYGTLGDAAKVIARDGILE 410
           +  +  +   S   + FSLAWD P++ F  K+  ++R+YT+F+G+ GD A  ++   +  
Sbjct: 405 VCVSSKLLPRSRCCLEFSLAWDMPKIMFGAKSQVHYRRYTRFFGSDGDVAPALSHYALCH 464

Query: 411 HGKWERQIEEWQKPIVEDKRLPEWYPVTLFNELYFLNSGGTIWTDGLPPLQNLSTISDQK 470
           +  WE +I  WQ P+++D+ LP WY   LFNELYFL  GGT+W +               
Sbjct: 465 YADWEDRISAWQNPVLDDRTLPAWYKSALFNELYFLADGGTVWLE--------------- 524

Query: 471 YFLQRSKSEPNGVPNGGHRYDVAIDILERMSQIFDQSHGGAGPSNAALGTRLLLPGEENV 530
                       VP                    D    G G S   L + L     ++ 
Sbjct: 525 ------------VP-------------------ADSLPEGLGGSMRQLRSTL-----QDY 584

Query: 531 GQLLLVEGSQYLMWNTYDVHFYSSFALIMLFPKLELSIQRDFAAAVLMHDPRKARIMSDG 590
           G+   +EG +Y M+NTYDVHFY+SFAL+ML+PKLELS+Q D A A L  D  + R +  G
Sbjct: 585 GRFGYLEGQEYRMYNTYDVHFYASFALVMLWPKLELSLQYDMALATLKEDLTRRRYLMSG 644

Query: 591 NWVPRKVIGAVPHDIGFND--PWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNF 650
              P K    +PHDIG  D  PW  VNAY + +   WKDL  KFVLQ+YRD   TGD+ F
Sbjct: 645 VVAPVKRRNVIPHDIGDPDDEPWLRVNAYLIHDTADWKDLNLKFVLQIYRDYYLTGDQGF 704

Query: 651 AKSVWPSVYVALAFME---QFDKDKDGMVENEGFPDQTYDTWTVKGVSAYCGGLWVAALQ 710
            + +WP   V LA ME   +FDKD+DG++EN G+ DQTYD W   G SAYCGGLW+AA+ 
Sbjct: 705 LEDMWP---VCLAVMESEMKFDKDQDGLIENGGYADQTYDAWVTTGPSAYCGGLWLAAVA 764

Query: 711 AASALASEVDDEAAADYFWVKYQKARDVY-GTLWNGSYFNYDNSGGPWSSSIQADQLAGQ 770
               +A     +   + F     + R+ Y   LWNG Y+NYD+S  P S SI +DQ AGQ
Sbjct: 765 VMVQMAVLCGAQDVQERFASILCRGREAYERLLWNGRYYNYDSSSHPQSRSIMSDQCAGQ 824

Query: 771 WYARACGL----CPITDEEKIRSALEKIYNFNVMKVKGGSRGAVNGMFPDGRVDTSMLQP 830
           W+ RACGL      +     +  AL+ I+  NV    GG+ GAVNGM P G  D S +Q 
Sbjct: 825 WFLRACGLGEGDTEVFPTLHVVRALQTIFELNVQAFAGGAMGAVNGMHPHGVPDRSSVQS 884

Query: 831 KEIWAGVTYSLAASMIQEGMVETGFLTAMGVHQAAWAQDGLGYSFQTPEAWDVNDKYRSL 890
            E+W GV Y LAA+MIQEG+   GF TA G ++  W  + LG +FQTPEA+     +RSL
Sbjct: 885 DEVWVGVVYGLAATMIQEGLTWEGFRTAEGCYRTVW--ERLGLAFQTPEAYCQQQVFRSL 896

Query: 891 GYMRPLAIWAMQWALSKPTLVKKKHKIPTK 902
            YMRPL+IWAMQ AL +    K +    T+
Sbjct: 945 AYMRPLSIWAMQLALQQQQHKKSRRPSVTQ 896

BLAST of Spg021484 vs. ExPASy Swiss-Prot
Match: Q5M868 (Non-lysosomal glucosylceramidase OS=Rattus norvegicus OX=10116 GN=Gba2 PE=2 SV=2)

HSP 1 Score: 559.7 bits (1441), Expect = 6.4e-158
Identity = 331/859 (38.53%), Postives = 461/859 (53.67%), Query Frame = 0

Query: 51  HLG-SLGYRLWRHGKEEAAKGRIPLFEFFSSQPITCHHGVSLGGIGAGSMGRSYRGEFQR 110
           H G  L Y  W + K +  K + P  + F+S P+   +G  LGGIG G++ R +RG+F R
Sbjct: 105 HFGMGLRYLKWWYRKTQVEK-KTPFIDMFNSVPLRQIYGCPLGGIGGGTITRGWRGQFCR 164

Query: 111 FQMFFGPSEEEPVLANQFSAFVSRPNGNKFSTVLCSAKPQKSKDGKQDGIESWDWNLSGE 170
           +Q+  G  + + V+A+QF   + R     +  VL    P          + SW+W L G 
Sbjct: 165 WQLNPGMYQHQTVIADQFIVCLRRDGRTVYQQVLSLELPSV--------LRSWNWGLCGY 224

Query: 171 NSTYHALFPRAWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNEGQTSA 230
            + YHAL+PRAWT+Y   P  ++ + CRQI+PI+PH+Y++SS PV VF +++ NEG  + 
Sbjct: 225 FAFYHALYPRAWTVYQ-LPGQNVTLTCRQITPILPHDYQDSSLPVGVFVWDVENEGDETL 284

Query: 231 EVTLLFTWAVSAAKSILCSFHGFILIQWSE------DG--AHGVLLHHKTANGRPTVTYA 290
           +V+++F     + ++ L          W+E      DG    G+LLHH T       T A
Sbjct: 285 DVSIMF-----SMRNGLGGEDDAAGGLWNEPFRLEQDGTTVQGLLLHHPTPPN--PYTMA 344

Query: 291 IAAEATDDVHVSVCPCFVISGDSEGISAKDMWQEIKNHGSFEKLGSVGAYEGSKPGSSIG 350
           +AA  T D  V+    F    DS G   + +WQ++   G  +     G    ++ G  + 
Sbjct: 345 VAARHTADTTVTYTTAF--DPDSTG---QQVWQDLLQDGQLD--SPAGQSTPTQRGEGVA 404

Query: 351 AAIAATVTIPSASARTVTFSLAWDCPEVKF--DGKTYHRQYTKFYGTLGDAAKVIARDGI 410
            A+ A+  +       + FSLAWD P + F   G+ ++R+YT+F+G+ GD A  ++   +
Sbjct: 405 GAVCASSKLLPRGRCCLEFSLAWDMPRIMFGAKGQVHYRRYTRFFGSDGDVAPALSHYAL 464

Query: 411 LEHGKWERQIEEWQKPIVEDKRLPEWYPVTLFNELYFLNSGGTIWTDGLPPLQNLSTISD 470
            ++  WE  I  WQ P+++D+ LP WY   LFNELYFL  GGT+W + +P          
Sbjct: 465 CQYAGWENSISAWQNPVLDDRSLPAWYKSALFNELYFLADGGTVWLE-VP---------- 524

Query: 471 QKYFLQRSKSEPNGVPNGGHRYDVAIDILERMSQIFDQSHGGAGPSNAALGTRLLLPGEE 530
                    S P  +  GG  Y                                L P  +
Sbjct: 525 -------EDSLPEEL--GGSMYQ-------------------------------LRPILQ 584

Query: 531 NVGQLLLVEGSQYLMWNTYDVHFYSSFALIMLFPKLELSIQRDFAAAVLMHDPRKARIMS 590
           + G+   +EG +Y M+NTYDVHFY+SFAL+ML+PKLELS+Q D A A    D  + R + 
Sbjct: 585 DYGRFGYLEGQEYRMYNTYDVHFYASFALVMLWPKLELSLQYDMALATFKEDLTRRRYLM 644

Query: 591 DGNWVPRKVIGAVPHDIGFND--PWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDK 650
            G   P K    +PHDIG  D  PW  VNAY + +   WKDL  KFVLQVYRD   TGD+
Sbjct: 645 SGVVAPVKRRNVIPHDIGDPDDEPWLRVNAYLIHDTADWKDLNLKFVLQVYRDYYLTGDQ 704

Query: 651 NFAKSVWPSVYVALAFME---QFDKDKDGMVENEGFPDQTYDTWTVKGVSAYCGGLWVAA 710
            F K +WP   V LA ME   +FDKD+DG++EN G+ DQTYD W   G SAYCGGLW+AA
Sbjct: 705 GFLKDMWP---VCLAVMESEMKFDKDQDGLIENGGYADQTYDGWVTTGPSAYCGGLWLAA 764

Query: 711 LQAASALASEVDDEAAADYFWVKYQKARDVY-GTLWNGSYFNYDNSGGPWSSSIQADQLA 770
           +     +A     +   D F     + R+ Y   LWNG Y+NYD+S  P S S+ +DQ A
Sbjct: 765 VAVMVQMAVLCGAQDVQDKFSSILCRGREAYERLLWNGRYYNYDSSSQPQSRSVMSDQCA 824

Query: 771 GQWYARACGL----CPITDEEKIRSALEKIYNFNVMKVKGGSRGAVNGMFPDGRVDTSML 830
           GQW+ RACGL      +     +  AL+ I+  NV    GG+ GAVNGM P G  D S +
Sbjct: 825 GQWFLRACGLGEGDTEVFPTLHVVRALKTIFELNVQAFAGGAMGAVNGMQPHGVPDRSSV 883

Query: 831 QPKEIWAGVTYSLAASMIQEGMVETGFLTAMGVHQAAWAQDGLGYSFQTPEAWDVNDKYR 889
           Q  E+W GV Y LAA+MIQEG+   GF TA G ++  W  + LG +FQTPEA+     +R
Sbjct: 885 QSDEVWVGVVYGLAATMIQEGLTWEGFRTAEGCYRTVW--ERLGLAFQTPEAYCQQRVFR 883

BLAST of Spg021484 vs. ExPASy Swiss-Prot
Match: Q7KT91 (Non-lysosomal glucosylceramidase OS=Drosophila melanogaster OX=7227 GN=CG33090 PE=1 SV=1)

HSP 1 Score: 471.5 bits (1212), Expect = 2.3e-131
Identity = 307/929 (33.05%), Postives = 455/929 (48.98%), Query Frame = 0

Query: 60  WRHGKEEAAKGRIPLFEFFSSQPITCHHGVSLGGIGAGSMGRSYRGEFQRFQMFFGPSEE 119
           W+  +E    GR    +++  +     +GV +GGIG G++GR Y GEF RFQM  G  E 
Sbjct: 93  WKVSRE----GRRVYMDYYYMENGKQIYGVPIGGIGGGTIGRGYAGEFCRFQMRPGIYEY 152

Query: 120 EPVLANQFSAFVSRPNG-NKFSTVL--CSAKPQKS-KDGKQDG----------------- 179
             VLANQF   +  P G   F ++L  CS + + S  DG  DG                 
Sbjct: 153 NVVLANQFIVTIKDPKGCTIFQSLLSKCSTRDKTSDPDGDPDGERTKCQLPNCSSRAKQP 212

Query: 180 IESWDWNLSGENSTYHALFPRAWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFT 239
           + +W  N+     +Y  L+PR+WT YD      +++ CRQ+SP+IPH Y+ESS P +VF 
Sbjct: 213 LSAWHSNIEDTRCSYTGLYPRSWTEYD-LSHYGVRLTCRQVSPVIPHEYRESSLPCAVFV 272

Query: 240 FNLSNEGQTSAEVTLLFTWAVSAAKSILCSFHGFILIQWSEDGAHGVLLHHKTANGRPTV 299
           +++ N      +V++ FT+          +  G      SE  A GV +  K +      
Sbjct: 273 WSVENVCDQERKVSITFTFKNGTGNKKQDAEGGAESQLISEGNAKGVSIRQKIS--EMPC 332

Query: 300 TYAIAAEATDDVHVSVCPCFVISGDSEGISAKDMWQEIKNHGSFEKLGSVGAYEGSKPGS 359
           +Y +A     ++ ++ CP F  +G+ E      +W ++K HG   +  +  A +      
Sbjct: 333 SYNLACRVLPEISITRCPQFDPAGNGE-----QLWAQLKEHGQLSEHPTSEALK----TK 392

Query: 360 SIGAAIAATVTIPSASARTVTFSLAWDCPEVKFDGK--TYHRQYTKFYGTLGDAAKVIAR 419
            IG A+   V +   ++  + F LAWD P+++F  K  T+ R YTK++   GD+   I  
Sbjct: 393 DIGVAVCGQVALKPMASHDLEFVLAWDMPKIQFPRKMQTHTRYYTKYFDDSGDSGPRICE 452

Query: 420 DGILEHGKWERQIEEWQKPIVEDKRLPEWYPVTLFNELYFLNSGGTIWTDGLPPLQNLST 479
             + ++  WER I+ WQ+PI+ D+ LP+WY   +FN+LYF++ GGTIW            
Sbjct: 453 YALRQYSTWERLIDAWQRPILNDETLPDWYKCAIFNQLYFISDGGTIWL----------- 512

Query: 480 ISDQKYFLQRSKSEPNGVPNGGHRYDVAIDILERMSQIFDQSHGGAGPSNAALGTRLLLP 539
                                                            +++LG  L   
Sbjct: 513 -----------------------------------------------KCDSSLGKELAYD 572

Query: 540 GEE-NVGQLLLVEGSQYLMWNTYDVHFYSSFALIMLFPKLELSIQRDF--AAAVLMHDPR 599
                 G+   +EG +Y M+NTYDVHFY+S AL  L+P L++S+Q DF  A A  ++D R
Sbjct: 573 DPRLAYGRFGYLEGHEYRMYNTYDVHFYASPALAHLWPNLQVSLQYDFKDAIAAELNDTR 632

Query: 600 KARIMSDGNWVPRKVIGAVPHDIGFND--PWFEVNAYNLLNVTRWKDLGSKFVLQVYRDV 659
           K  ++ DG  +PRKV   VPHD+G  D  P+  +N YN+ +V  WKDL +KFVLQVYRD 
Sbjct: 633 K--MLYDGKVMPRKVKNCVPHDLGDPDEEPFTLINCYNIHDVNDWKDLNTKFVLQVYRDY 692

Query: 660 VATGDKNFAKSVWPSVYVALAFME------------------------------------ 719
               +   A+S   S + ++ F++                                    
Sbjct: 693 YVLNELAQAQSDNASKFSSIEFIDKESLYELYSQDNKRKNSADEKQQNRKSASMYINETN 752

Query: 720 ---------------------------QFDKDKDGMVENEGFPDQTYDTWTVKGVSAYCG 779
                                      ++DKD DG++EN   PDQTYD+W + G SAYC 
Sbjct: 753 GKVYLMDAIGYLKAMYASCKAIMERTIEYDKDNDGLIENTKMPDQTYDSWVMDGPSAYCS 812

Query: 780 GLWVAALQAASALASEVDD-EAAADYFWVKYQKARDVYGTLWNGSYFNYDNSGGPWSSSI 839
           GLW+AALQA SA+A+ +D       Y  +  +  R +   LWNGSY+ +D S      +I
Sbjct: 813 GLWLAALQAMSAMATILDQPNDCLRYQDILEKGKRSLEEKLWNGSYYRFDLSHS-HRDTI 872

Query: 840 QADQLAGQWYARACGL-CPITDEEKIRSALEKIYNFNVMKVKGGSRGAVNGMFPD----- 889
            ADQL G WY ++CG    I  +E +R+AL++IY+ NVM    G+ GA NG   +     
Sbjct: 873 MADQLCGHWYLKSCGFDYEIYPKENVRTALKRIYDNNVMGFHEGNIGAANGFIANASEPT 932

BLAST of Spg021484 vs. ExPASy TrEMBL
Match: A0A6J1GAL9 (Non-lysosomal glucosylceramidase OS=Cucurbita moschata OX=3662 GN=LOC111452445 PE=3 SV=1)

HSP 1 Score: 1723.0 bits (4461), Expect = 0.0e+00
Identity = 833/959 (86.86%), Postives = 886/959 (92.39%), Query Frame = 0

Query: 1   MENGNKEEGNNGASSTKVDPAKLPSLTWQRRLDFTGKSPESFSFTLNDMWHLGSLGYRLW 60
           MENG KE+ +NGASST VDPAKLPSLTWQR+LDFTGKSPESFSFTLND WHLG LGYRLW
Sbjct: 1   MENGCKEDADNGASSTMVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRLW 60

Query: 61  RHGKEEAAKGRIPLFEFFSSQPITCHHGVSLGGIGAGSMGRSYRGEFQRFQMFFGPSEEE 120
           RHGKEEAAKGRIPL++FF+S PITC+HGVSLGGIGAGS+GRSYRGEFQRFQMF+GPSE+ 
Sbjct: 61  RHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSEDV 120

Query: 121 PVLANQFSAFVSRPNGNKFSTVLCSAKPQKSKDGKQDGIESWDWNLSGENSTYHALFPRA 180
           PVLANQFSAFVSRPNGNKFSTVLCSA+PQKSKDGK DGIE+WDWNLSGE STYHALFPRA
Sbjct: 121 PVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPRA 180

Query: 181 WTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNEGQTSAEVTLLFTWAVS 240
           WTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSN+G TSAEVTLLFTWA S
Sbjct: 181 WTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWANS 240

Query: 241 AAKSILCSFHGF--------ILIQWSEDGAHGVLLHHKTANGRPTVTYAIAAEATDDVHV 300
              +   + H F          +  +EDGAHGVLLHHKTANGRPTVTYAI AEATDDVHV
Sbjct: 241 VGGNSGFTGHHFNSKIGVKAEFVSSAEDGAHGVLLHHKTANGRPTVTYAITAEATDDVHV 300

Query: 301 SVCPCFVISGDSEGISAKDMWQEIKNHGSFEKLGSVGAYEGSKPGSSIGAAIAATVTIPS 360
           SVCP FVISGDSEGISAKDMWQE+K HGSF++LGS G +EGSKPGSSIGAAIAA V+IPS
Sbjct: 301 SVCPRFVISGDSEGISAKDMWQEVKKHGSFDELGSSGPHEGSKPGSSIGAAIAAKVSIPS 360

Query: 361 ASARTVTFSLAWDCPEVKFDGKTYHRQYTKFYGTLGDAAKVIARDGILEHGKWERQIEEW 420
           +S RTVTFSLAWDCPEVKFDGKTYHR+YT FYGTLG+AAK IARD ILEHGKWE QIE W
Sbjct: 361 SSTRTVTFSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEAW 420

Query: 421 QKPIVEDKRLPEWYPVTLFNELYFLNSGGTIWTDGLPPLQNLSTISDQKYFLQRSKSEPN 480
           Q+PIVEDKRLPEWYPVTLFNELYFLNSGGT+WTDGLPPLQNLSTIS QK FL+R+KSEPN
Sbjct: 421 QRPIVEDKRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSEPN 480

Query: 481 GVPNGGHRYDVAIDILERMSQIFDQSHGGAGPSNAALGTRLLLPGEENVGQLLLVEGSQY 540
           G PNG HR DVA++ILERMSQIFDQ+HGGAGPSNAALGTRLL   +ENVGQLLLVEGS+Y
Sbjct: 481 GAPNGDHRLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSEY 540

Query: 541 LMWNTYDVHFYSSFALIMLFPKLELSIQRDFAAAVLMHDPRKARIMSDGNWVPRKVIGAV 600
           LMWNT+DVHFYSSFALIMLFPKLEL IQRDFAAAVLMHDPRKA+IMSDG + PRKVIGAV
Sbjct: 541 LMWNTFDVHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGAV 600

Query: 601 PHDIGFNDPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALA 660
           PHDIGFNDPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALA
Sbjct: 601 PHDIGFNDPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALA 660

Query: 661 FMEQFDKDKDGMVENEGFPDQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDDEAAAD 720
           FM+QFDKDKDGM+ENEGFPDQTYD WTVKGVSAYCGGLWVAALQAASALA EVDDEAAA 
Sbjct: 661 FMDQFDKDKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAAH 720

Query: 721 YFWVKYQKARDVYGTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPITDEEKI 780
           YFW+KYQKAR VY TLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPI DEEKI
Sbjct: 721 YFWIKYQKARGVYDTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPIVDEEKI 780

Query: 781 RSALEKIYNFNVMKVKGGSRGAVNGMFPDGRVDTSMLQPKEIWAGVTYSLAASMIQEGMV 840
           RS LEKI+NFNVMKVKGG+RGAVNGMFPDGRVD S+LQPKEIWAGVTYS+AASMIQEGMV
Sbjct: 781 RSTLEKIFNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGMV 840

Query: 841 ETGFLTAMGVHQAAWAQDGLGYSFQTPEAWDVNDKYRSLGYMRPLAIWAMQWALSKPTLV 900
           ETGF TAMG+HQAAW QDGLGYSFQTPEAWD++DKYRSLGYMRPLAIWAMQWA+SKPTL+
Sbjct: 841 ETGFQTAMGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTLL 900

Query: 901 KKKHKIPTKVLSEIEESS-FAAQHAAFLKVASLLKLPSKEAAHRSLVEVAYDFICKRSA 951
            KKHK+P K LSE EES  F+ QHAAFLKVASLLKLPS+EA  RS+VEVAYDFICKRSA
Sbjct: 901 -KKHKVPRKELSESEESCLFSTQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA 958

BLAST of Spg021484 vs. ExPASy TrEMBL
Match: A0A6J1GAM9 (Non-lysosomal glucosylceramidase OS=Cucurbita moschata OX=3662 GN=LOC111452445 PE=3 SV=1)

HSP 1 Score: 1721.8 bits (4458), Expect = 0.0e+00
Identity = 833/952 (87.50%), Postives = 885/952 (92.96%), Query Frame = 0

Query: 1   MENGNKEEGNNGASSTK-VDPAKLPSLTWQRRLDFTGKSPESFSFTLNDMWHLGSLGYRL 60
           MENG KE+ +NGASST  VDPAKLPSLTWQR+LDFTGKSPESFSFTLND WHLG LGYRL
Sbjct: 1   MENGCKEDADNGASSTMVVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRL 60

Query: 61  WRHGKEEAAKGRIPLFEFFSSQPITCHHGVSLGGIGAGSMGRSYRGEFQRFQMFFGPSEE 120
           WRHGKEEAAKGRIPL++FF+S PITC+HGVSLGGIGAGS+GRSYRGEFQRFQMF+GPSE+
Sbjct: 61  WRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSED 120

Query: 121 EPVLANQFSAFVSRPNGNKFSTVLCSAKPQKSKDGKQDGIESWDWNLSGENSTYHALFPR 180
            PVLANQFSAFVSRPNGNKFSTVLCSA+PQKSKDGK DGIE+WDWNLSGE STYHALFPR
Sbjct: 121 VPVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPR 180

Query: 181 AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNEGQTSAEVTLLFTWAV 240
           AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSN+G TSAEVTLLFTWA 
Sbjct: 181 AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWAN 240

Query: 241 SAAKSILCSFHGFILIQWSEDGAHGVLLHHKTANGRPTVTYAIAAEATDDVHVSVCPCFV 300
           S   +   + H F     +EDGAHGVLLHHKTANGRPTVTYAI AEATDDVHVSVCP FV
Sbjct: 241 SVGGNSGFTGHHFNSKIGAEDGAHGVLLHHKTANGRPTVTYAITAEATDDVHVSVCPRFV 300

Query: 301 ISGDSEGISAKDMWQEIKNHGSFEKLGSVGAYEGSKPGSSIGAAIAATVTIPSASARTVT 360
           ISGDSEGISAKDMWQE+K HGSF++LGS G +EGSKPGSSIGAAIAA V+IPS+S RTVT
Sbjct: 301 ISGDSEGISAKDMWQEVKKHGSFDELGSSGPHEGSKPGSSIGAAIAAKVSIPSSSTRTVT 360

Query: 361 FSLAWDCPEVKFDGKTYHRQYTKFYGTLGDAAKVIARDGILEHGKWERQIEEWQKPIVED 420
           FSLAWDCPEVKFDGKTYHR+YT FYGTLG+AAK IARD ILEHGKWE QIE WQ+PIVED
Sbjct: 361 FSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEAWQRPIVED 420

Query: 421 KRLPEWYPVTLFNELYFLNSGGTIWTDGLPPLQNLSTISDQKYFLQRSKSEPNGVPNGGH 480
           KRLPEWYPVTLFNELYFLNSGGT+WTDGLPPLQNLSTIS QK FL+R+KSEPNG PNG H
Sbjct: 421 KRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSEPNGAPNGDH 480

Query: 481 RYDVAIDILERMSQIFDQSHGGAGPSNAALGTRLLLPGEENVGQLLLVEGSQYLMWNTYD 540
           R DVA++ILERMSQIFDQ+HGGAGPSNAALGTRLL   +ENVGQLLLVEGS+YLMWNT+D
Sbjct: 481 RLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSEYLMWNTFD 540

Query: 541 VHFYSSFALIMLFPKLELSIQRDFAAAVLMHDPRKARIMSDGNWVPRKVIGAVPHDIGFN 600
           VHFYSSFALIMLFPKLEL IQRDFAAAVLMHDPRKA+IMSDG + PRKVIGAVPHDIGFN
Sbjct: 541 VHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGAVPHDIGFN 600

Query: 601 DPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMEQFDK 660
           DPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFM+QFDK
Sbjct: 601 DPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMDQFDK 660

Query: 661 DKDGMVENEGFPDQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDDEAAADYFWVKYQ 720
           DKDGM+ENEGFPDQTYD WTVKGVSAYCGGLWVAALQAASALA EVDDEAAA YFW+KYQ
Sbjct: 661 DKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAAHYFWIKYQ 720

Query: 721 KARDVYGTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPITDEEKIRSALEKI 780
           KAR VY TLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPI DEEKIRS LEKI
Sbjct: 721 KARGVYDTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPIVDEEKIRSTLEKI 780

Query: 781 YNFNVMKVKGGSRGAVNGMFPDGRVDTSMLQPKEIWAGVTYSLAASMIQEGMVETGFLTA 840
           +NFNVMKVKGG+RGAVNGMFPDGRVD S+LQPKEIWAGVTYS+AASMIQEGMVETGF TA
Sbjct: 781 FNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGMVETGFQTA 840

Query: 841 MGVHQAAWAQDGLGYSFQTPEAWDVNDKYRSLGYMRPLAIWAMQWALSKPTLVKKKHKIP 900
           MG+HQAAW QDGLGYSFQTPEAWD++DKYRSLGYMRPLAIWAMQWA+SKPTL+ KKHK+P
Sbjct: 841 MGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTLL-KKHKVP 900

Query: 901 TKVLSEIEESS-FAAQHAAFLKVASLLKLPSKEAAHRSLVEVAYDFICKRSA 951
            K LSE EES  F+ QHAAFLKVASLLKLPS+EA  RS+VEVAYDFICKRSA
Sbjct: 901 RKELSESEESCLFSTQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA 951

BLAST of Spg021484 vs. ExPASy TrEMBL
Match: A0A6J1GBB8 (Non-lysosomal glucosylceramidase OS=Cucurbita moschata OX=3662 GN=LOC111452445 PE=3 SV=1)

HSP 1 Score: 1718.4 bits (4449), Expect = 0.0e+00
Identity = 833/960 (86.77%), Postives = 886/960 (92.29%), Query Frame = 0

Query: 1   MENGNKEEGNNGASSTK-VDPAKLPSLTWQRRLDFTGKSPESFSFTLNDMWHLGSLGYRL 60
           MENG KE+ +NGASST  VDPAKLPSLTWQR+LDFTGKSPESFSFTLND WHLG LGYRL
Sbjct: 1   MENGCKEDADNGASSTMVVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRL 60

Query: 61  WRHGKEEAAKGRIPLFEFFSSQPITCHHGVSLGGIGAGSMGRSYRGEFQRFQMFFGPSEE 120
           WRHGKEEAAKGRIPL++FF+S PITC+HGVSLGGIGAGS+GRSYRGEFQRFQMF+GPSE+
Sbjct: 61  WRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSED 120

Query: 121 EPVLANQFSAFVSRPNGNKFSTVLCSAKPQKSKDGKQDGIESWDWNLSGENSTYHALFPR 180
            PVLANQFSAFVSRPNGNKFSTVLCSA+PQKSKDGK DGIE+WDWNLSGE STYHALFPR
Sbjct: 121 VPVLANQFSAFVSRPNGNKFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPR 180

Query: 181 AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNEGQTSAEVTLLFTWAV 240
           AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSN+G TSAEVTLLFTWA 
Sbjct: 181 AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWAN 240

Query: 241 SAAKSILCSFHGF--------ILIQWSEDGAHGVLLHHKTANGRPTVTYAIAAEATDDVH 300
           S   +   + H F          +  +EDGAHGVLLHHKTANGRPTVTYAI AEATDDVH
Sbjct: 241 SVGGNSGFTGHHFNSKIGVKAEFVSSAEDGAHGVLLHHKTANGRPTVTYAITAEATDDVH 300

Query: 301 VSVCPCFVISGDSEGISAKDMWQEIKNHGSFEKLGSVGAYEGSKPGSSIGAAIAATVTIP 360
           VSVCP FVISGDSEGISAKDMWQE+K HGSF++LGS G +EGSKPGSSIGAAIAA V+IP
Sbjct: 301 VSVCPRFVISGDSEGISAKDMWQEVKKHGSFDELGSSGPHEGSKPGSSIGAAIAAKVSIP 360

Query: 361 SASARTVTFSLAWDCPEVKFDGKTYHRQYTKFYGTLGDAAKVIARDGILEHGKWERQIEE 420
           S+S RTVTFSLAWDCPEVKFDGKTYHR+YT FYGTLG+AAK IARD ILEHGKWE QIE 
Sbjct: 361 SSSTRTVTFSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIARDAILEHGKWEGQIEA 420

Query: 421 WQKPIVEDKRLPEWYPVTLFNELYFLNSGGTIWTDGLPPLQNLSTISDQKYFLQRSKSEP 480
           WQ+PIVEDKRLPEWYPVTLFNELYFLNSGGT+WTDGLPPLQNLSTIS QK FL+R+KSEP
Sbjct: 421 WQRPIVEDKRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLERTKSEP 480

Query: 481 NGVPNGGHRYDVAIDILERMSQIFDQSHGGAGPSNAALGTRLLLPGEENVGQLLLVEGSQ 540
           NG PNG HR DVA++ILERMSQIFDQ+HGGAGPSNAALGTRLL   +ENVGQLLLVEGS+
Sbjct: 481 NGAPNGDHRLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSE 540

Query: 541 YLMWNTYDVHFYSSFALIMLFPKLELSIQRDFAAAVLMHDPRKARIMSDGNWVPRKVIGA 600
           YLMWNT+DVHFYSSFALIMLFPKLEL IQRDFAAAVLMHDPRKA+IMSDG + PRKVIGA
Sbjct: 541 YLMWNTFDVHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGA 600

Query: 601 VPHDIGFNDPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVAL 660
           VPHDIGFNDPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVAL
Sbjct: 601 VPHDIGFNDPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVAL 660

Query: 661 AFMEQFDKDKDGMVENEGFPDQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDDEAAA 720
           AFM+QFDKDKDGM+ENEGFPDQTYD WTVKGVSAYCGGLWVAALQAASALA EVDDEAAA
Sbjct: 661 AFMDQFDKDKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAA 720

Query: 721 DYFWVKYQKARDVYGTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPITDEEK 780
            YFW+KYQKAR VY TLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPI DEEK
Sbjct: 721 HYFWIKYQKARGVYDTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPIVDEEK 780

Query: 781 IRSALEKIYNFNVMKVKGGSRGAVNGMFPDGRVDTSMLQPKEIWAGVTYSLAASMIQEGM 840
           IRS LEKI+NFNVMKVKGG+RGAVNGMFPDGRVD S+LQPKEIWAGVTYS+AASMIQEGM
Sbjct: 781 IRSTLEKIFNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGM 840

Query: 841 VETGFLTAMGVHQAAWAQDGLGYSFQTPEAWDVNDKYRSLGYMRPLAIWAMQWALSKPTL 900
           VETGF TAMG+HQAAW QDGLGYSFQTPEAWD++DKYRSLGYMRPLAIWAMQWA+SKPTL
Sbjct: 841 VETGFQTAMGIHQAAWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTL 900

Query: 901 VKKKHKIPTKVLSEIEESS-FAAQHAAFLKVASLLKLPSKEAAHRSLVEVAYDFICKRSA 951
           + KKHK+P K LSE EES  F+ QHAAFLKVASLLKLPS+EA  RS+VEVAYDFICKRSA
Sbjct: 901 L-KKHKVPRKELSESEESCLFSTQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA 959

BLAST of Spg021484 vs. ExPASy TrEMBL
Match: A0A6J1I8G5 (Non-lysosomal glucosylceramidase OS=Cucurbita maxima OX=3661 GN=LOC111471931 PE=3 SV=1)

HSP 1 Score: 1709.9 bits (4427), Expect = 0.0e+00
Identity = 828/952 (86.97%), Postives = 879/952 (92.33%), Query Frame = 0

Query: 1   MENGNKEEGNNGASSTK-VDPAKLPSLTWQRRLDFTGKSPESFSFTLNDMWHLGSLGYRL 60
           M+NG KEE +NG SST  VDPAKLPSLTWQR+LDFTGKSPESFSFTLND WHLG LGYRL
Sbjct: 1   MKNGCKEEADNGDSSTMVVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRL 60

Query: 61  WRHGKEEAAKGRIPLFEFFSSQPITCHHGVSLGGIGAGSMGRSYRGEFQRFQMFFGPSEE 120
           WRHGKEEAAKGRIPL++FF+S PITC+HGVSLGGIGAGS+GRSYRGEFQRFQMF+GPSE+
Sbjct: 61  WRHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSED 120

Query: 121 EPVLANQFSAFVSRPNGNKFSTVLCSAKPQKSKDGKQDGIESWDWNLSGENSTYHALFPR 180
            PVLANQFSAFVSRPNGN+FSTVLCSA+PQKSKDGK DGIE+WDWNLSGE STYHALFPR
Sbjct: 121 VPVLANQFSAFVSRPNGNRFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPR 180

Query: 181 AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNEGQTSAEVTLLFTWAV 240
           AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSN+G TSAEVTLLFTWA 
Sbjct: 181 AWTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWAN 240

Query: 241 SAAKSILCSFHGFILIQWSEDGAHGVLLHHKTANGRPTVTYAIAAEATDDVHVSVCPCFV 300
           S   +   + H F     +EDGAHGVLLHHKTANGRPTVTYAI AEATDDVHVSVCP FV
Sbjct: 241 SVGGNSGFTGHHFNSKIGAEDGAHGVLLHHKTANGRPTVTYAITAEATDDVHVSVCPRFV 300

Query: 301 ISGDSEGISAKDMWQEIKNHGSFEKLGSVGAYEGSKPGSSIGAAIAATVTIPSASARTVT 360
           ISGDSEGISAKDMWQE+KNHGSF+K G+ G +E SKPGSSIGAAIAA V+IPS+S  TVT
Sbjct: 301 ISGDSEGISAKDMWQEVKNHGSFDKFGNSGPHEESKPGSSIGAAIAAKVSIPSSSTCTVT 360

Query: 361 FSLAWDCPEVKFDGKTYHRQYTKFYGTLGDAAKVIARDGILEHGKWERQIEEWQKPIVED 420
           FSLAWDCPEVKFDGKTYHR+YT FYGTLG+AAK I RD ILEHGKWE QIE WQ+PIVED
Sbjct: 361 FSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIGRDAILEHGKWEGQIEAWQRPIVED 420

Query: 421 KRLPEWYPVTLFNELYFLNSGGTIWTDGLPPLQNLSTISDQKYFLQRSKSEPNGVPNGGH 480
           KRLPEWYPVTLFNELYFLNSGGT+WTDGLPPLQNLSTIS QK FLQR+KSEPNG PNG H
Sbjct: 421 KRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLQRTKSEPNGAPNGDH 480

Query: 481 RYDVAIDILERMSQIFDQSHGGAGPSNAALGTRLLLPGEENVGQLLLVEGSQYLMWNTYD 540
           R DVA++ILERMSQIFDQ+HGGAGPSNAALGTRLL   +ENVGQLLLVEGS+YLMWNT+D
Sbjct: 481 RLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSEYLMWNTFD 540

Query: 541 VHFYSSFALIMLFPKLELSIQRDFAAAVLMHDPRKARIMSDGNWVPRKVIGAVPHDIGFN 600
           VHFYSSFALIMLFPKLEL IQRDFAAAVLMHDPRKA+IMSDG + PRKVIGAVPHDIGFN
Sbjct: 541 VHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGAVPHDIGFN 600

Query: 601 DPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMEQFDK 660
           DPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFM+QFDK
Sbjct: 601 DPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMDQFDK 660

Query: 661 DKDGMVENEGFPDQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDDEAAADYFWVKYQ 720
           DKDGM+ENEGFPDQTYD WTVKGVSAYCGGLWVAALQAASALA EVDDEAAA YFW+KYQ
Sbjct: 661 DKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAAHYFWIKYQ 720

Query: 721 KARDVYGTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPITDEEKIRSALEKI 780
           KAR VY TLWNGSYFNYDNS GPWSSSIQADQLAGQWYARACGLCPI DEEKIRS LEKI
Sbjct: 721 KARGVYDTLWNGSYFNYDNSSGPWSSSIQADQLAGQWYARACGLCPIVDEEKIRSTLEKI 780

Query: 781 YNFNVMKVKGGSRGAVNGMFPDGRVDTSMLQPKEIWAGVTYSLAASMIQEGMVETGFLTA 840
           +NFNVMKVKGG+RGAVNGMFPDGRVD S+LQPKEIWAGVTYS+AASMIQEGMVETGF TA
Sbjct: 781 FNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGMVETGFQTA 840

Query: 841 MGVHQAAWAQDGLGYSFQTPEAWDVNDKYRSLGYMRPLAIWAMQWALSKPTLVKKKHKIP 900
           MG+HQA W QDGLGYSFQTPEAWD++DKYRSLGYMRPLAIWAMQWA+SKPTL+ KKHK+P
Sbjct: 841 MGIHQAVWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTLL-KKHKVP 900

Query: 901 TKVLSEIEESS-FAAQHAAFLKVASLLKLPSKEAAHRSLVEVAYDFICKRSA 951
            K LSE EES  FA QHAAFLKVASLLKLPS+EA  RS+VEVAYDFICKRSA
Sbjct: 901 RKELSESEESCLFATQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA 951

BLAST of Spg021484 vs. ExPASy TrEMBL
Match: A0A6J1IB63 (Non-lysosomal glucosylceramidase OS=Cucurbita maxima OX=3661 GN=LOC111471931 PE=3 SV=1)

HSP 1 Score: 1709.5 bits (4426), Expect = 0.0e+00
Identity = 828/959 (86.34%), Postives = 879/959 (91.66%), Query Frame = 0

Query: 1   MENGNKEEGNNGASSTKVDPAKLPSLTWQRRLDFTGKSPESFSFTLNDMWHLGSLGYRLW 60
           M+NG KEE +NG SST VDPAKLPSLTWQR+LDFTGKSPESFSFTLND WHLG LGYRLW
Sbjct: 1   MKNGCKEEADNGDSSTMVDPAKLPSLTWQRKLDFTGKSPESFSFTLNDKWHLGPLGYRLW 60

Query: 61  RHGKEEAAKGRIPLFEFFSSQPITCHHGVSLGGIGAGSMGRSYRGEFQRFQMFFGPSEEE 120
           RHGKEEAAKGRIPL++FF+S PITC+HGVSLGGIGAGS+GRSYRGEFQRFQMF+GPSE+ 
Sbjct: 61  RHGKEEAAKGRIPLYDFFASPPITCYHGVSLGGIGAGSIGRSYRGEFQRFQMFYGPSEDV 120

Query: 121 PVLANQFSAFVSRPNGNKFSTVLCSAKPQKSKDGKQDGIESWDWNLSGENSTYHALFPRA 180
           PVLANQFSAFVSRPNGN+FSTVLCSA+PQKSKDGK DGIE+WDWNLSGE STYHALFPRA
Sbjct: 121 PVLANQFSAFVSRPNGNRFSTVLCSARPQKSKDGKNDGIEAWDWNLSGEKSTYHALFPRA 180

Query: 181 WTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNEGQTSAEVTLLFTWAVS 240
           WTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSN+G TSAEVTLLFTWA S
Sbjct: 181 WTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNKGPTSAEVTLLFTWANS 240

Query: 241 AAKSILCSFHGF--------ILIQWSEDGAHGVLLHHKTANGRPTVTYAIAAEATDDVHV 300
              +   + H F             +EDGAHGVLLHHKTANGRPTVTYAI AEATDDVHV
Sbjct: 241 VGGNSGFTGHHFNSKIGVKAEFASSAEDGAHGVLLHHKTANGRPTVTYAITAEATDDVHV 300

Query: 301 SVCPCFVISGDSEGISAKDMWQEIKNHGSFEKLGSVGAYEGSKPGSSIGAAIAATVTIPS 360
           SVCP FVISGDSEGISAKDMWQE+KNHGSF+K G+ G +E SKPGSSIGAAIAA V+IPS
Sbjct: 301 SVCPRFVISGDSEGISAKDMWQEVKNHGSFDKFGNSGPHEESKPGSSIGAAIAAKVSIPS 360

Query: 361 ASARTVTFSLAWDCPEVKFDGKTYHRQYTKFYGTLGDAAKVIARDGILEHGKWERQIEEW 420
           +S  TVTFSLAWDCPEVKFDGKTYHR+YT FYGTLG+AAK I RD ILEHGKWE QIE W
Sbjct: 361 SSTCTVTFSLAWDCPEVKFDGKTYHRRYTNFYGTLGEAAKTIGRDAILEHGKWEGQIEAW 420

Query: 421 QKPIVEDKRLPEWYPVTLFNELYFLNSGGTIWTDGLPPLQNLSTISDQKYFLQRSKSEPN 480
           Q+PIVEDKRLPEWYPVTLFNELYFLNSGGT+WTDGLPPLQNLSTIS QK FLQR+KSEPN
Sbjct: 421 QRPIVEDKRLPEWYPVTLFNELYFLNSGGTMWTDGLPPLQNLSTISHQKIFLQRTKSEPN 480

Query: 481 GVPNGGHRYDVAIDILERMSQIFDQSHGGAGPSNAALGTRLLLPGEENVGQLLLVEGSQY 540
           G PNG HR DVA++ILERMSQIFDQ+HGGAGPSNAALGTRLL   +ENVGQLLLVEGS+Y
Sbjct: 481 GAPNGDHRLDVAVEILERMSQIFDQAHGGAGPSNAALGTRLLPTLQENVGQLLLVEGSEY 540

Query: 541 LMWNTYDVHFYSSFALIMLFPKLELSIQRDFAAAVLMHDPRKARIMSDGNWVPRKVIGAV 600
           LMWNT+DVHFYSSFALIMLFPKLEL IQRDFAAAVLMHDPRKA+IMSDG + PRKVIGAV
Sbjct: 541 LMWNTFDVHFYSSFALIMLFPKLELGIQRDFAAAVLMHDPRKAKIMSDGKYYPRKVIGAV 600

Query: 601 PHDIGFNDPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALA 660
           PHDIGFNDPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALA
Sbjct: 601 PHDIGFNDPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALA 660

Query: 661 FMEQFDKDKDGMVENEGFPDQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDDEAAAD 720
           FM+QFDKDKDGM+ENEGFPDQTYD WTVKGVSAYCGGLWVAALQAASALA EVDDEAAA 
Sbjct: 661 FMDQFDKDKDGMIENEGFPDQTYDVWTVKGVSAYCGGLWVAALQAASALAYEVDDEAAAH 720

Query: 721 YFWVKYQKARDVYGTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPITDEEKI 780
           YFW+KYQKAR VY TLWNGSYFNYDNS GPWSSSIQADQLAGQWYARACGLCPI DEEKI
Sbjct: 721 YFWIKYQKARGVYDTLWNGSYFNYDNSSGPWSSSIQADQLAGQWYARACGLCPIVDEEKI 780

Query: 781 RSALEKIYNFNVMKVKGGSRGAVNGMFPDGRVDTSMLQPKEIWAGVTYSLAASMIQEGMV 840
           RS LEKI+NFNVMKVKGG+RGAVNGMFPDGRVD S+LQPKEIWAGVTYS+AASMIQEGMV
Sbjct: 781 RSTLEKIFNFNVMKVKGGTRGAVNGMFPDGRVDRSILQPKEIWAGVTYSVAASMIQEGMV 840

Query: 841 ETGFLTAMGVHQAAWAQDGLGYSFQTPEAWDVNDKYRSLGYMRPLAIWAMQWALSKPTLV 900
           ETGF TAMG+HQA W QDGLGYSFQTPEAWD++DKYRSLGYMRPLAIWAMQWA+SKPTL+
Sbjct: 841 ETGFQTAMGIHQAVWGQDGLGYSFQTPEAWDIDDKYRSLGYMRPLAIWAMQWAISKPTLL 900

Query: 901 KKKHKIPTKVLSEIEESS-FAAQHAAFLKVASLLKLPSKEAAHRSLVEVAYDFICKRSA 951
            KKHK+P K LSE EES  FA QHAAFLKVASLLKLPS+EA  RS+VEVAYDFICKRSA
Sbjct: 901 -KKHKVPRKELSESEESCLFATQHAAFLKVASLLKLPSEEAKPRSVVEVAYDFICKRSA 958

BLAST of Spg021484 vs. TAIR 10
Match: AT5G49900.1 (Beta-glucosidase, GBA2 type family protein )

HSP 1 Score: 1197.6 bits (3097), Expect = 0.0e+00
Identity = 575/939 (61.24%), Postives = 719/939 (76.57%), Query Frame = 0

Query: 13  ASSTKVDPAKLPSLTWQRRLDFTGKSPESFSFTLNDMWHLGSLGYRLWRHGKEEAAKGRI 72
           +S TKVDPA   SLTWQR++D   K+P  F+ ++ +++ L  +G RLW   +EEAAKGR+
Sbjct: 18  SSDTKVDPAVPASLTWQRKIDSDVKAPREFNLSVKEIFQLAPVGIRLWFLCREEAAKGRL 77

Query: 73  PLFEFFSSQPITCHHGVSLGGIGAGSMGRSYRGEFQRFQMFFGPSEEEPVLANQFSAFVS 132
              + FS   +T  HGV LGGIGAGS+GRS++GEFQR+Q+F    E+EPVLANQFSAFVS
Sbjct: 78  AFIDPFSKHSVTSSHGVPLGGIGAGSIGRSFKGEFQRWQLFPPKCEDEPVLANQFSAFVS 137

Query: 133 RPNGNKFSTVLCSAKPQKSKDGKQDGIESWDWNLSGENSTYHALFPRAWTIYDGEPDPDL 192
           R NG K+S+VLC   P+  K   + GI SWDWNL G+ STYHAL+PR+WT+Y+GEPDP+L
Sbjct: 138 RANGKKYSSVLCPRNPKLDKQDSESGIGSWDWNLKGDKSTYHALYPRSWTMYEGEPDPEL 197

Query: 193 KIVCRQISPIIPHNYKESSFPVSVFTFNLSNEGQTSAEVTLLFTWAVSAAKSILCSFHGF 252
           +IVCRQ+SP IPHNYKESSFPVSVFTF L N G T+A+VTLLFTWA S       S   +
Sbjct: 198 RIVCRQVSPFIPHNYKESSFPVSVFTFTLHNLGNTTADVTLLFTWANSVGGDSEFSGGHY 257

Query: 253 ILIQWSEDGAHGVLLHHKTANGRPTVTYAIAAEATDDVHVSVCPCFVISGDSEGISAKDM 312
                  DG  GVLLHHKTANG P+++YAI+A+ATD V VS CP F++SG  +GI+AKDM
Sbjct: 258 NSKITMNDGVQGVLLHHKTANGLPSLSYAISAQATDGVSVSACPFFIVSGKQDGITAKDM 317

Query: 313 WQEIKNHGSFEKLGSVGAYEGSKPGSSIGAAIAATVTIPSASARTVTFSLAWDCPEVKF- 372
           WQ +K +GSF+ L +  A   S  GSSIGAA+AA+VT+    +R VTFSLAWDCPEV+F 
Sbjct: 318 WQAVKENGSFDHLKASEASMQSDHGSSIGAAVAASVTVLPGESRIVTFSLAWDCPEVQFP 377

Query: 373 DGKTYHRQYTKFYGTLGDAAKVIARDGILEHGKWERQIEEWQKPIVEDKRLPEWYPVTLF 432
            GK Y R+YTKFYG  GDAA  IA D IL H +WE  IE+WQ+PI+EDKRLP WYPVTLF
Sbjct: 378 SGKIYSRRYTKFYGNNGDAAAQIAHDAILGHSQWESWIEDWQRPILEDKRLPAWYPVTLF 437

Query: 433 NELYFLNSGGTIWTDGLPPLQNLSTISDQKYFLQRSKSEPNGVPNGGHRYDVAIDILERM 492
           NELY+LNSGGT+WTDG  P+ +L+ + ++K+ L +S+       +  H+ D A+ +LE+M
Sbjct: 438 NELYYLNSGGTLWTDGSSPVHSLAGVREKKFSLDKSQLGLKNDIDVPHQNDTAVSVLEKM 497

Query: 493 SQIFDQSHGGAGPSNAALGTRLLLPGEENVGQLLLVEGSQYLMWNTYDVHFYSSFALIML 552
           +   ++ H     SN+A GT+LL  GEEN+G  L +EG +Y MWNTYDVHFY+SFAL+ML
Sbjct: 498 ASTLEELHAST-TSNSAFGTKLLEEGEENIGHFLYLEGIEYRMWNTYDVHFYASFALVML 557

Query: 553 FPKLELSIQRDFAAAVLMHDPRKARIMSDGNWVPRKVIGAVPHDIGFNDPWFEVNAYNLL 612
           FPKLELSIQRDFAAAV++HDP K + +S+G WV RKV+GAVPHD+G NDPWFEVN Y L 
Sbjct: 558 FPKLELSIQRDFAAAVMLHDPTKVKTLSEGQWVQRKVLGAVPHDLGINDPWFEVNGYTLH 617

Query: 613 NVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMEQFDKDKDGMVENEGFP 672
           N  RWKDL  KFVLQVYRDVVATGDK FA +VWPSVYVA+A+M QFDKD DGM+ENEGFP
Sbjct: 618 NTDRWKDLNPKFVLQVYRDVVATGDKKFASAVWPSVYVAMAYMAQFDKDGDGMIENEGFP 677

Query: 673 DQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDDEAAADYFWVKYQKARDVY-GTLWN 732
           DQTYDTW+  GVSAYCGGLWVAALQAASALA  V D+ + DYFW K+QKA+ VY   LWN
Sbjct: 678 DQTYDTWSASGVSAYCGGLWVAALQAASALARVVGDKNSQDYFWSKFQKAKVVYEKKLWN 737

Query: 733 GSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPITDEEKIRSALEKIYNFNVMKVKGG 792
           GSYFNYDNSG  +SS+IQADQLAGQWYARA GL PI DE+K R+ALEK+YN+NVMK+K G
Sbjct: 738 GSYFNYDNSGSQYSSTIQADQLAGQWYARASGLLPIVDEDKARTALEKVYNYNVMKIKDG 797

Query: 793 SRGAVNGMFPDGRVDTSMLQPKEIWAGVTYSLAASMIQEGMVETGFLTAMGVHQAAWAQD 852
            RGAVNGM P+G+VDT+ +Q +EIW+GVTY+L+A+MIQEG+VE  F TA G+++AAW++ 
Sbjct: 798 KRGAVNGMHPNGKVDTASMQSREIWSGVTYALSATMIQEGLVEMAFQTASGIYEAAWSET 857

Query: 853 GLGYSFQTPEAWDVNDKYRSLGYMRPLAIWAMQWALSKPTLVKKKHKI-PTKVLSEIEES 912
           GLGYSFQTPE+W+  D+YRSL YMRPLAIWAMQWAL+K +  +++  + P +   E+E S
Sbjct: 858 GLGYSFQTPESWNTVDEYRSLTYMRPLAIWAMQWALTKTSQKQEQLGLEPEQQEPELEPS 917

Query: 913 SFAAQHAAFLKVASLLKLPSKEAAHRSLVEVAYDFICKR 949
           S       F +V+ LL LP+ EA+ +S ++  +D+ C+R
Sbjct: 918 SSMKHDIGFSRVSRLLSLPN-EASAKSTLQTLFDYTCRR 954

BLAST of Spg021484 vs. TAIR 10
Match: AT1G33700.1 (Beta-glucosidase, GBA2 type family protein )

HSP 1 Score: 1146.7 bits (2965), Expect = 0.0e+00
Identity = 567/936 (60.58%), Postives = 684/936 (73.08%), Query Frame = 0

Query: 22  KLPSLTWQRRLDFTGKSPESFSFTLNDMWHLGSLGYRLWRHGKEEAAKGRIPLFEFFSSQ 81
           KLP  +W+R+L+   K+P  F  +  D  HL  LGYRLWRH K+EAAKGR  +F+ F   
Sbjct: 16  KLPPFSWERKLNSQAKTPSEFKLSKRDHMHLFPLGYRLWRHTKDEAAKGRASIFDIFRKH 75

Query: 82  PITCHHGVSLGGIGAGSMGRSYRGEFQRFQMFFGPSEEEPVLANQFSAFVSRPNGNKFST 141
            IT  HGV LGGIG+GS+GRSY+GEFQ+F++F    EE P+L NQFSAFVSRP G K ST
Sbjct: 76  HITGDHGVPLGGIGSGSIGRSYKGEFQQFKLFPKICEEAPILTNQFSAFVSRPGGVKHST 135

Query: 142 VLCSAKPQKSKD---------GKQDGIESWDWNLSGENSTYHALFPRAWTIYDGEPDPDL 201
           VLC +KPQ  KD             GIESWDWN++GE STYHAL+PR+WT+YDGEPDP+L
Sbjct: 136 VLCPSKPQVVKDNGGYLCQGQAPNMGIESWDWNMTGEKSTYHALYPRSWTVYDGEPDPEL 195

Query: 202 KIVCRQISPIIPHNYKESSFPVSVFTFNLSNEGQTSAEVTLLFTWAVSAAKSILCSFHGF 261
           +IV RQ+SP IPHNY+ESS PVSVF F ++N G   A VTLLFTW  S   +   +   F
Sbjct: 196 RIVSRQVSPFIPHNYEESSLPVSVFNFTVTNTGAEPAIVTLLFTWENSVGGASGLTGQHF 255

Query: 262 ILIQWSEDGAHGVLLHHKTANGRPTVTYAIAAEATDDVHVSVCPCFVISGDSEG-ISAKD 321
                ++DG H V L HKTANG P V+YAIAA+ T+DV VS CPCF++SG +   I+A D
Sbjct: 256 NSTMKAKDGVHAVALQHKTANGHPPVSYAIAAKETEDVRVSSCPCFIVSGTTPNQITAGD 315

Query: 322 MWQEIKNHGSFEKLGSVGAYEGSKPGSSIGAAIAATVTIPSASARTVTFSLAWDCPEVKF 381
           MW EIK + SF+KL S  A   SKPG+SIGAAIAA V +P    RTVTFSL+WDCPE +F
Sbjct: 316 MWDEIKKNASFDKLTS-NACSPSKPGTSIGAAIAAKVKVPPGCDRTVTFSLSWDCPEARF 375

Query: 382 DGKTYHRQYTKFYGTLGDAAKVIARDGILEHGKWERQIEEWQKPIVEDKRLPEWYPVTLF 441
           D KTYHR+YT+FYG+LG+AA  +A D +L   +WE QIEEWQ P++ D  LPEWY VTLF
Sbjct: 376 DEKTYHRRYTRFYGSLGNAAVAMAHDALLNFPEWETQIEEWQAPVLADTTLPEWYRVTLF 435

Query: 442 NELYFLNSGGTIWTDGLPPLQNLSTISDQKYFLQRSKSEPNGVPNGGHRYDVAIDILERM 501
           NELY+ NSGGT+WTDGLPP Q+L +I  +K  L  S  + N         +VA+DIL R+
Sbjct: 436 NELYYFNSGGTMWTDGLPPKQSLDSIGRRKISLGLSTIDKNDQDQN----NVALDILGRI 495

Query: 502 SQIFDQSHGGAGPSNAALGTRLLLPGEENVGQLLLVEGSQYLMWNTYDVHFYSSFALIML 561
             +  Q H     SNAALG  ++    EN+GQ L +EG QYLM+NTYDVHFYSSFAL+ML
Sbjct: 496 DAVCSQIHAPL-TSNAALGNTMIQNTTENIGQFLYLEGVQYLMYNTYDVHFYSSFALLML 555

Query: 562 FPKLELSIQRDFAAAVLMHDPRKARIMSDGNWVPRKVIGAVPHDIGFNDPWFEVNAYNLL 621
           FPK+ELSIQRDFAAAVLMHD  K ++MS G +V RKV+GAVPHDIG NDPWFE+NAYNL 
Sbjct: 556 FPKIELSIQRDFAAAVLMHDSSKKQVMSSGEFVTRKVLGAVPHDIGLNDPWFELNAYNLF 615

Query: 622 NVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMEQFDKDKDGMVENEGFP 681
           N  RWKDL SKFVLQVYRDVVATGD NFAK+VWPSVY A+A+++QFDKD DGM+ENEGFP
Sbjct: 616 NTDRWKDLNSKFVLQVYRDVVATGDLNFAKAVWPSVYTAIAYLDQFDKDGDGMIENEGFP 675

Query: 682 DQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDDEAAADYFWVKYQKARDVYGTLWNG 741
           DQTYD W+  GVSAYCGGLWVAALQA SALA E+ D  AA YF  KY+KAR VY  LWNG
Sbjct: 676 DQTYDAWSCSGVSAYCGGLWVAALQAGSALAREIGDNGAAVYFNAKYEKARSVYEKLWNG 735

Query: 742 SYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPITDEEKIRSALEKIYNFNVMKVKGGS 801
           SYFNYDNS    SSSI ADQ+AGQWYARACGL PI  EE I+ ALE +Y+FNVM+V+ G+
Sbjct: 736 SYFNYDNSRSGSSSSILADQMAGQWYARACGLKPIAKEEWIKKALETVYDFNVMRVRDGT 795

Query: 802 RGAVNGMFPDGRVDTSMLQPKEIWAGVTYSLAASMIQEGMVETGFLTAMGVHQAAWAQDG 861
           RGAVNGM PDGRVDTS +  +E+WAG TYS+AA MIQEG+ + GF TA G+++AAW+  G
Sbjct: 796 RGAVNGMLPDGRVDTSTMVSREVWAGTTYSVAACMIQEGLADKGFRTASGIYEAAWSDRG 855

Query: 862 LGYSFQTPEAWDVNDKYRSLGYMRPLAIWAMQWALSKPTLVKKKHKIPTKVLSEIEESSF 921
           LG +FQTPEAW  ND+YRSL YMRPLAIW +QWA + P    ++ ++  +   E   S  
Sbjct: 856 LGCAFQTPEAWTTNDEYRSLCYMRPLAIWGIQWAHTMPK-PNREQEVSLRPQEEDATSVL 915

Query: 922 AAQHAAFLKVASLLKLPSKEAAHRSLVEVAYDFICK 948
             QHA F+KVA  LK  +K   HR+ ++ AY+   K
Sbjct: 916 FQQHAGFIKVAHYLK-NTKGKDHRNRLQTAYETFLK 943

BLAST of Spg021484 vs. TAIR 10
Match: AT1G33700.2 (Beta-glucosidase, GBA2 type family protein )

HSP 1 Score: 1146.7 bits (2965), Expect = 0.0e+00
Identity = 567/936 (60.58%), Postives = 684/936 (73.08%), Query Frame = 0

Query: 22  KLPSLTWQRRLDFTGKSPESFSFTLNDMWHLGSLGYRLWRHGKEEAAKGRIPLFEFFSSQ 81
           KLP  +W+R+L+   K+P  F  +  D  HL  LGYRLWRH K+EAAKGR  +F+ F   
Sbjct: 16  KLPPFSWERKLNSQAKTPSEFKLSKRDHMHLFPLGYRLWRHTKDEAAKGRASIFDIFRKH 75

Query: 82  PITCHHGVSLGGIGAGSMGRSYRGEFQRFQMFFGPSEEEPVLANQFSAFVSRPNGNKFST 141
            IT  HGV LGGIG+GS+GRSY+GEFQ+F++F    EE P+L NQFSAFVSRP G K ST
Sbjct: 76  HITGDHGVPLGGIGSGSIGRSYKGEFQQFKLFPKICEEAPILTNQFSAFVSRPGGVKHST 135

Query: 142 VLCSAKPQKSKD---------GKQDGIESWDWNLSGENSTYHALFPRAWTIYDGEPDPDL 201
           VLC +KPQ  KD             GIESWDWN++GE STYHAL+PR+WT+YDGEPDP+L
Sbjct: 136 VLCPSKPQVVKDNGGYLCQGQAPNMGIESWDWNMTGEKSTYHALYPRSWTVYDGEPDPEL 195

Query: 202 KIVCRQISPIIPHNYKESSFPVSVFTFNLSNEGQTSAEVTLLFTWAVSAAKSILCSFHGF 261
           +IV RQ+SP IPHNY+ESS PVSVF F ++N G   A VTLLFTW  S   +   +   F
Sbjct: 196 RIVSRQVSPFIPHNYEESSLPVSVFNFTVTNTGAEPAIVTLLFTWENSVGGASGLTGQHF 255

Query: 262 ILIQWSEDGAHGVLLHHKTANGRPTVTYAIAAEATDDVHVSVCPCFVISGDSEG-ISAKD 321
                ++DG H V L HKTANG P V+YAIAA+ T+DV VS CPCF++SG +   I+A D
Sbjct: 256 NSTMKAKDGVHAVALQHKTANGHPPVSYAIAAKETEDVRVSSCPCFIVSGTTPNQITAGD 315

Query: 322 MWQEIKNHGSFEKLGSVGAYEGSKPGSSIGAAIAATVTIPSASARTVTFSLAWDCPEVKF 381
           MW EIK + SF+KL S  A   SKPG+SIGAAIAA V +P    RTVTFSL+WDCPE +F
Sbjct: 316 MWDEIKKNASFDKLTS-NACSPSKPGTSIGAAIAAKVKVPPGCDRTVTFSLSWDCPEARF 375

Query: 382 DGKTYHRQYTKFYGTLGDAAKVIARDGILEHGKWERQIEEWQKPIVEDKRLPEWYPVTLF 441
           D KTYHR+YT+FYG+LG+AA  +A D +L   +WE QIEEWQ P++ D  LPEWY VTLF
Sbjct: 376 DEKTYHRRYTRFYGSLGNAAVAMAHDALLNFPEWETQIEEWQAPVLADTTLPEWYRVTLF 435

Query: 442 NELYFLNSGGTIWTDGLPPLQNLSTISDQKYFLQRSKSEPNGVPNGGHRYDVAIDILERM 501
           NELY+ NSGGT+WTDGLPP Q+L +I  +K  L  S  + N         +VA+DIL R+
Sbjct: 436 NELYYFNSGGTMWTDGLPPKQSLDSIGRRKISLGLSTIDKNDQDQN----NVALDILGRI 495

Query: 502 SQIFDQSHGGAGPSNAALGTRLLLPGEENVGQLLLVEGSQYLMWNTYDVHFYSSFALIML 561
             +  Q H     SNAALG  ++    EN+GQ L +EG QYLM+NTYDVHFYSSFAL+ML
Sbjct: 496 DAVCSQIHAPL-TSNAALGNTMIQNTTENIGQFLYLEGVQYLMYNTYDVHFYSSFALLML 555

Query: 562 FPKLELSIQRDFAAAVLMHDPRKARIMSDGNWVPRKVIGAVPHDIGFNDPWFEVNAYNLL 621
           FPK+ELSIQRDFAAAVLMHD  K ++MS G +V RKV+GAVPHDIG NDPWFE+NAYNL 
Sbjct: 556 FPKIELSIQRDFAAAVLMHDSSKKQVMSSGEFVTRKVLGAVPHDIGLNDPWFELNAYNLF 615

Query: 622 NVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMEQFDKDKDGMVENEGFP 681
           N  RWKDL SKFVLQVYRDVVATGD NFAK+VWPSVY A+A+++QFDKD DGM+ENEGFP
Sbjct: 616 NTDRWKDLNSKFVLQVYRDVVATGDLNFAKAVWPSVYTAIAYLDQFDKDGDGMIENEGFP 675

Query: 682 DQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDDEAAADYFWVKYQKARDVYGTLWNG 741
           DQTYD W+  GVSAYCGGLWVAALQA SALA E+ D  AA YF  KY+KAR VY  LWNG
Sbjct: 676 DQTYDAWSCSGVSAYCGGLWVAALQAGSALAREIGDNGAAVYFNAKYEKARSVYEKLWNG 735

Query: 742 SYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPITDEEKIRSALEKIYNFNVMKVKGGS 801
           SYFNYDNS    SSSI ADQ+AGQWYARACGL PI  EE I+ ALE +Y+FNVM+V+ G+
Sbjct: 736 SYFNYDNSRSGSSSSILADQMAGQWYARACGLKPIAKEEWIKKALETVYDFNVMRVRDGT 795

Query: 802 RGAVNGMFPDGRVDTSMLQPKEIWAGVTYSLAASMIQEGMVETGFLTAMGVHQAAWAQDG 861
           RGAVNGM PDGRVDTS +  +E+WAG TYS+AA MIQEG+ + GF TA G+++AAW+  G
Sbjct: 796 RGAVNGMLPDGRVDTSTMVSREVWAGTTYSVAACMIQEGLADKGFRTASGIYEAAWSDRG 855

Query: 862 LGYSFQTPEAWDVNDKYRSLGYMRPLAIWAMQWALSKPTLVKKKHKIPTKVLSEIEESSF 921
           LG +FQTPEAW  ND+YRSL YMRPLAIW +QWA + P    ++ ++  +   E   S  
Sbjct: 856 LGCAFQTPEAWTTNDEYRSLCYMRPLAIWGIQWAHTMPK-PNREQEVSLRPQEEDATSVL 915

Query: 922 AAQHAAFLKVASLLKLPSKEAAHRSLVEVAYDFICK 948
             QHA F+KVA  LK  +K   HR+ ++ AY+   K
Sbjct: 916 FQQHAGFIKVAHYLK-NTKGKDHRNRLQTAYETFLK 943

BLAST of Spg021484 vs. TAIR 10
Match: AT4G10060.1 (Beta-glucosidase, GBA2 type family protein )

HSP 1 Score: 1097.4 bits (2837), Expect = 0.0e+00
Identity = 548/951 (57.62%), Postives = 682/951 (71.71%), Query Frame = 0

Query: 2   ENGNKEEGNNGASSTKVDPAKLPSLTWQRRLDFTGKSPESFSFTLNDMWHLGSLGYRLWR 61
           +NG+ E   +   +  V   +LP +TWQR+L+   K+P  F  ++ D+ HL  LGYRLWR
Sbjct: 3   KNGHTE---SELQTQMVGNERLPQVTWQRKLNSKVKNPSEFKMSIRDVLHLFPLGYRLWR 62

Query: 62  HGKEEAAKGRIPLFEFFSSQPITCHHGVSLGGIGAGSMGRSYRGEFQRFQMFFGPSEEEP 121
           + KEEA KGR  +++ F  + +   HGV LGGIG GS+GRSY+GEFQ+F++F    EE P
Sbjct: 63  YTKEEAKKGRFSMYDIFKKRHVRGDHGVPLGGIGGGSIGRSYKGEFQQFKLFPKICEEAP 122

Query: 122 VLANQFSAFVSRPNGNKFSTVLCSAKPQKSKDGKQD-GIESWDWNLSGENSTYHALFPRA 181
           +L NQFS FVSRP G  +STVLC  KP+  K   +D GIESWDWN+ G+ STYHAL+PR+
Sbjct: 123 ILTNQFSVFVSRPGGLSYSTVLCPTKPKSVKGKTEDLGIESWDWNMEGDKSTYHALYPRS 182

Query: 182 WTIYDGEPDPDLKIVCRQISPIIPHNYKESSFPVSVFTFNLSNEGQTSAEVTLLFTWAVS 241
           WT+Y+ EPDP+L+IV RQ+SP IPHNYKESS PVSVF F +SN G+  A VTLLFTW  S
Sbjct: 183 WTVYN-EPDPELRIVSRQVSPFIPHNYKESSLPVSVFEFTMSNLGKEEATVTLLFTWENS 242

Query: 242 AAKSILCSFHGFILIQWSEDGAHGVLLHHKTANGRPTVTYAIAAEATDDVHVSVCPCFVI 301
              +   +   F       DG H ++LHHKT NG P VTYAIAA+ T+DVHVS CPCF++
Sbjct: 243 VGGASGLTGEHFNSTIMERDGVHAIVLHHKTGNGHPPVTYAIAAQETEDVHVSECPCFLV 302

Query: 302 SGDS-EGISAKDMWQEIKNHGSFEKLGSVGAYEGSKPGSSIGAAIAATVTIPSASARTVT 361
           SG S + I+AK+MW EIK + SF++L S      S+PG+SIGAAIAA V +P    RTVT
Sbjct: 303 SGHSPKEITAKEMWDEIKKNKSFDELNSEPG-SPSRPGTSIGAAIAAKVKVPPGCDRTVT 362

Query: 362 FSLAWDCPEVKFDGKTYHRQYTKFYGTLGDAAKVIARDGILEHGKWERQIEEWQKPIVED 421
           FSL+WDCPEV+F+ KTYHR+YTKFYG LGDAA  +ARD +L +  WE QIE WQ PI+ D
Sbjct: 363 FSLSWDCPEVRFNEKTYHRRYTKFYGNLGDAAVKMARDALLNYVDWESQIEAWQSPILSD 422

Query: 422 KRLPEWYPVTLFNELYFLNSGGTIWTDGLPPLQNLSTISDQKYFLQRSKSEPNGVPNGGH 481
             LP+WY VTLFNELY+ NSGGTIWTDGLPP          K  ++RSK           
Sbjct: 423 TTLPDWYRVTLFNELYYFNSGGTIWTDGLPP----------KESIERSK------VTNTE 482

Query: 482 RYDVAIDILERMSQIFDQSHGGAGPSNAALGTRLLLPGEENVGQLLLVEGSQYLMWNTYD 541
           + D+ ID+ ++++ + +Q +     ++           EEN+GQ + +EG +YLM+NTYD
Sbjct: 483 QNDIVIDLFQKINAVCEQIYSPQSSNS-----------EENIGQFIYLEGIEYLMYNTYD 542

Query: 542 VHFYSSFALIMLFPKLELSIQRDFAAAVLMHDPRKARIMSDGNWVPRKVIGAVPHDIGFN 601
           VHFYSSFAL+ LFPKL LSIQRDFAA VL+ DP K +IMS G WV RK++G+VPHDIG N
Sbjct: 543 VHFYSSFALLSLFPKLALSIQRDFAATVLIQDPTKKKIMSSGEWVTRKLLGSVPHDIGLN 602

Query: 602 DPWFEVNAYNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMEQFDK 661
           DPW E+N YN  N  RWKDL +KFVLQVYRDVVAT D++FAK+VWPSVY A+A+++QFDK
Sbjct: 603 DPWLELNEYNFFNTDRWKDLNAKFVLQVYRDVVATNDQSFAKAVWPSVYTAVAYLDQFDK 662

Query: 662 DKDGMVENEGFPDQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDDEAAADYFWVKYQ 721
           D+DGM+ENEGFPDQTYD W+V GVSAYCGGLWVAALQAASA AS V + A A YF  KY+
Sbjct: 663 DEDGMIENEGFPDQTYDAWSVTGVSAYCGGLWVAALQAASAFASIVGENAVAIYFNAKYE 722

Query: 722 KARDVYGTLWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPITDEEKIRSALEKI 781
           KA+ VY  LWNGSYFNYD+SG   SSSI ADQLAGQWYARACGL PIT EE I+ ALE I
Sbjct: 723 KAKIVYEKLWNGSYFNYDDSGSGSSSSILADQLAGQWYARACGLKPITKEEWIKKALETI 782

Query: 782 YNFNVMKVKGGSRGAVNGMFPDGRVDTSMLQPKEIWAGVTYSLAASMIQEGMVETGFLTA 841
           Y FNVMKVKGG+RGAVNGM  +G+VDT+ L  KE+WAG TYS+AA MIQEG  E GF TA
Sbjct: 783 YEFNVMKVKGGTRGAVNGMSTEGKVDTNSLVSKEVWAGTTYSVAACMIQEGQREKGFQTA 842

Query: 842 MGVHQAAWAQDGLGYSFQTPEAWDVNDKYRSLGYMRPLAIWAMQWALSK-PTLVKKKHKI 901
            G+++A W+  GL  SFQTPEAW++ND+YRSL YMRPLAIWA+QWAL++  +  ++K K+
Sbjct: 843 SGIYEAIWSDRGLSCSFQTPEAWNMNDEYRSLCYMRPLAIWAIQWALTRTQSFGEEKQKL 902

Query: 902 PTKVLSEIEESS--FAAQHAAFLKVASLLKLPSKEAAHRSLVEVAYDFICK 948
              V  + EE S     QH  F  VA  +K+      HRS ++  Y+ + K
Sbjct: 903 ---VAGDEEEESNLLLRQHKGFKDVARFVKIVPTSNVHRSRLQHTYETVLK 918

BLAST of Spg021484 vs. TAIR 10
Match: AT3G24180.1 (Beta-glucosidase, GBA2 type family protein )

HSP 1 Score: 856.3 bits (2211), Expect = 2.4e-248
Identity = 437/886 (49.32%), Postives = 580/886 (65.46%), Query Frame = 0

Query: 19  DPAKLPSLTWQRRLDFTGKSPESFSFTLNDMWHLGSLGYRLWRHGKEEAAKGRIPLFEFF 78
           D A  P   W+RRL+      + F+ T  +   +  LG RLW + +EEA+ GR    + F
Sbjct: 31  DSAAPPPHAWRRRLNCHANILKEFTITFREAIKMVRLGIRLWSYVREEASHGRKAPIDPF 90

Query: 79  SSQPI--TCHHGVSLGGIGAGSMGRSYRGEFQRFQMFFGPSEEEPVLANQFSAFVSRPNG 138
           + +    +   GV LGG+G+GS+ R +RGEF+++Q+  G  +  P+++NQFS F+SR  G
Sbjct: 91  TKENCKPSASQGVPLGGMGSGSISRGFRGEFKQWQITPGTCDPSPMMSNQFSIFISRDGG 150

Query: 139 N-KFSTVLCSAKPQKSKDGKQDGIESWDWNLSGENSTYHALFPRAWTIYDGEPDPDLKIV 198
           + K+++VL   +       +  G+ SW WNL+G++STYHALFPRAWTIYDGEPDP+LKI 
Sbjct: 151 HKKYASVLSPGQHGSLGKSRDKGLSSWGWNLNGQHSTYHALFPRAWTIYDGEPDPELKIS 210

Query: 199 CRQISPIIPHNYKESSFPVSVFTFNLSNEGQTSAEVTLLFTWAVSAAKSILCSFHGFILI 258
           CRQISP IP+NY++SS P +VF + L N G+  A+V+LLFTWA S   +   S       
Sbjct: 211 CRQISPFIPNNYRDSSLPAAVFVYTLVNTGKERAKVSLLFTWANSMGGTSHMSGGHVNEP 270

Query: 259 QWSEDGAHGVLLHHKTANGRPTVTYAIAAEATDDVHVSVCPCFVISGDSEGISAKDMWQE 318
              EDG  GVLLHHKT  G P VT+AIAA  T +V+V+V PCF +S DS   +AKDMW  
Sbjct: 271 FIGEDGVSGVLLHHKTGKGNPPVTFAIAASETQNVNVTVLPCFGLSEDS-SFTAKDMWDT 330

Query: 319 IKNHGSFEKLG-SVGAYEGSKPGSSIGAAIAATVTIPSASARTVTFSLAWDCPEVKFD-G 378
           ++  G F++   + G    S  G +I AA++A+  + +    TV+F+L+W  P+VKF  G
Sbjct: 331 MEQDGKFDQENFNSGPSTPSLAGDTICAAVSASAWVEAHGKCTVSFALSWSSPKVKFSKG 390

Query: 379 KTYHRQYTKFYGTLGDAAKVIARDGILEHGKWERQIEEWQKPIVEDKRLPEWYPVTLFNE 438
            TY R+YTKFYGT   AA  +  D +  + +WE  IE WQ PI+ D+RLPEWY  TLFNE
Sbjct: 391 STYDRRYTKFYGTSPRAALDLVHDALTNYKRWEEDIEAWQNPILRDERLPEWYKFTLFNE 450

Query: 439 LYFLNSGGTIWTDGLPPLQNLSTISDQKYFLQRSKSEPNGVPNGGHRYD------VAIDI 498
           LYFL +GGT+W D    L        Q+  L  S  +  G+     R D      V +  
Sbjct: 451 LYFLVAGGTVWIDS-SSLNANGNSQHQQSGLGNSDGKVGGLDINDQRNDLGNGNSVGVKS 510

Query: 499 LERMSQIFDQSHGGAGPSNAALGTRLLLPGEENVGQLLLVEGSQYLMWNTYDVHFYSSFA 558
            + +S I ++        N        +   ++VG+ L +EG +Y+MW TYDVHFY+S+A
Sbjct: 511 NDEVSAIHNR--------NGLFVDTPHVDDGDDVGRFLYLEGVEYVMWCTYDVHFYASYA 570

Query: 559 LIMLFPKLELSIQRDFAAAVLMHDPRKARIMSDGNWVPRKVIGAVPHDIGFNDPWFEVNA 618
           L+MLFPK+EL+IQRDFA AVL  D RK + +++GN   RKV GAVPHD+G +DPW E+NA
Sbjct: 571 LLMLFPKIELNIQRDFAKAVLSEDGRKVKFLAEGNVGIRKVRGAVPHDLGMHDPWNEMNA 630

Query: 619 YNLLNVTRWKDLGSKFVLQVYRDVVATGDKNFAKSVWPSVYVALAFMEQFDKDKDGMVEN 678
           YN+ + +RWKDL  KFVLQVYRD  ATGD  F   VWP+V  A+ +MEQFD+D D ++EN
Sbjct: 631 YNIHDTSRWKDLNPKFVLQVYRDFAATGDYQFGIDVWPAVRAAMEYMEQFDRDNDDLIEN 690

Query: 679 EGFPDQTYDTWTVKGVSAYCGGLWVAALQAASALASEVDDEAAADYFWVKYQKARDVYGT 738
           +GFPDQTYDTWTV GVSAYCG LW+AALQAA+A+A ++ D+  A+    K+  A+    T
Sbjct: 691 DGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMALQIGDKFFAELCKNKFLNAKAALET 750

Query: 739 -LWNGSYFNYDNSGGPWSSSIQADQLAGQWYARACGLCPITDEEKIRSALEKIYNFNVMK 798
            LWNGSYFNYD+     S SIQ DQLAGQWYA + GL P+ +E KIRS ++KI++FNVMK
Sbjct: 751 KLWNGSYFNYDSGSSSNSKSIQTDQLAGQWYAASSGLPPLFEESKIRSTMQKIFDFNVMK 810

Query: 799 VKGGSRGAVNGMFPDGRVDTSMLQPKEIWAGVTYSLAASMIQEGMVETGFLTAMGVHQAA 858
            KGG  GAVNGM PDG+VD + +Q +EIW GVTY+ AA+MI  GM E GF TA G+  A 
Sbjct: 811 TKGGKMGAVNGMHPDGKVDDTCMQSREIWTGVTYAAAATMILSGMEEQGFTTAEGIFTAG 870

Query: 859 WAQDGLGYSFQTPEAWDVNDKYRSLGYMRPLAIWAMQWALSKPTLV 893
           W+++G GY FQTPE W ++  YRSL YMRPLAIW MQWALS P  +
Sbjct: 871 WSEEGFGYWFQTPEGWTMDGHYRSLIYMRPLAIWGMQWALSLPKAI 906