Spg021447 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg021447
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Pectin lyase-like superfamily protein
Location	scaffold9: 4544700 .. 4546234 (-)
RNA-Seq Expression	Spg021447
Synteny	Spg021447

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGGTCCATGCAAAAGTCCTATCGAAATTCAACTTCAGGAACATTGCAGGCCCCAATAGATCTAACTGGAGAAGGTTGGGTTAGTTTTCAAATATAGTCGACTACTTTACATTAACAGGTGGTGGAGTTTTAGATGGACAAGGAAAAGTAGCTTGGGAAAAAAATGATTGTGACAAAAATCCAAAATGCGACAGAATTCCCATGGTAAAGCACACTTCAATCATCGAATTAGTGTTCTTAGTACCTTTCTTTCTTTTCTAGTTCTATATTGATTAAACTATATAATCCACATGGTTAGATTTTTAATAAAGGGCCAAACCAACAACTCACAATTTCTCTTAATAAATATATATGTATGATCTTTTTTCTTTTTATCAAAAATCGATCGCAGACTTTGAAGCTCAGCTTTATCACAAATTCAATAGTGAGTACATAACCTCTAAAGATAGCAAGAATTTTTCATGTTAATGTTTTGGGTTGTAAAAACCTTACCTTCCACAGTGTGAACATAAATGCACCCGGAGATAGTATCAATAGTGATGGAATTCACATTGGTCCGTTCTCTTGGAATTAATGTCATAAATACAAACATTGGCACTGGAGATGATTGTATCTCACTTGGAGATGGAAGCAAGCAAGTAACAATTACTAATGTGACATGTGGTCCGGGACATGGTATTAGTGTAGGAAGTTTAGGAAAGTACAATGCTGAAGAACCAGTTGAGGGAATCATAGTTAAGAATTGTACATTGATGAATACATCAAATGGCGTAAGAATCAAATCATGGCCTGCTTCTCCTGTTGCTGGAACAGCTTCTGATATGCACTATGAAGATATTATTATGGTTAATGTCAGTAATCCCATTCTCATAGATCAAGAGTATTGTCCATACAACCAATGTAATAAAAAGGTACTAAACTCATGGATTTTTATTTTTTATTTTTTTATTGCATTTATAAATGTTTCAATTAAACTGAAGAAGCCTTGTTCTGTCTCTTTCTTTCCAGAGTCCATCCAAAGTCAAGATTAGTAAGGTTACTTTCAAGAATATCAGAGGTACTTCAGGTAATGCACTTGCCATCAAACTTGTTTGTAGTTCTCAGGTTCCATGTGAAGGTGTAGAGCTAGCTGACATTGACCTCACTTACAATGGAAGTGAAGGCCCTATTACATCTCAATGTTTAAATGTGAAGCCTACTATTTCTGGAAAGCAAAACCCTCCTGCATGTTCTTCTCCTGCAGCTCCTATCCTAGCTTAAACTAATTTTGTAGAGTTGACTATTTGATGCAGAAATCTTGTTACTTTTTTTATTTATTATTATATACTAAAGCTTATAGTCTAGATGATACAATTTCTTTTTTTTTTTTTTTTGTAACTTCATATTGTTTAACACCCAATTTCTTATAACAAATAATTGGGTGACAGAGGGTTTTCCATCCCTTATATTTATAATCCTTTTTTTACAATAGAAGACATGTCATTAAGCTCAATTAATCCGTCAAAAAATGTCTTGTTTAGATTGTTTTGCTATAAA

mRNA sequence

ATGCACCCGGAGATAGTATCAATAGTGATGGAATTCACATTGGTCCGTTCTCTTGGAATTAatgtcataaatacaaacattggcactggagatgattgtatctcacttggagatggaagcaagcaagtaacaattactaatgtgacatgtggtccgggacatggtattagtgtaggaagtttaggaaagtacaatgctgaagaaccagttgagggaatcatagttaagaattgtacattgatgaatacatcaaatggcgtaagaatcaaatcatggcctgcttctcctgttgctggaacagcttctgatatgcactatgaagatattattatggttaatgtcagtaatcccattctcatagatcaagagtattgtccatacaaccaatgtaataaaaagagtccatccaaagtcaagattagtaaggttactttcaagaatatcagaggtacttcaggtaatgcacttgccatcaaacttgtttgtagttctcaggttccatgtgaaggtgtagagctagctgacattgacctcacttacaatggaagtgaaggccctattacatctcaatgtttaaatgtgaagcctactatttctggaaagcaaaaccctcctgcatgttcttctcctgcagctcctatcctagcttaa

Coding sequence (CDS)

ATGCACCCGGAGATAGTATCAATAGTGATGGAATTCACATTGGTCCGTTCTCTTGGAATTAATGTCATAAATACAAACATTGGCACTGGAGATGATTGTATCTCACTTGGAGATGGAAGCAAGCAAGTAACAATTACTAATGTGACATGTGGTCCGGGACATGGTATTAGTGTAGGAAGTTTAGGAAAGTACAATGCTGAAGAACCAGTTGAGGGAATCATAGTTAAGAATTGTACATTGATGAATACATCAAATGGCGTAAGAATCAAATCATGGCCTGCTTCTCCTGTTGCTGGAACAGCTTCTGATATGCACTATGAAGATATTATTATGGTTAATGTCAGTAATCCCATTCTCATAGATCAAGAGTATTGTCCATACAACCAATGTAATAAAAAGAGTCCATCCAAAGTCAAGATTAGTAAGGTTACTTTCAAGAATATCAGAGGTACTTCAGGTAATGCACTTGCCATCAAACTTGTTTGTAGTTCTCAGGTTCCATGTGAAGGTGTAGAGCTAGCTGACATTGACCTCACTTACAATGGAAGTGAAGGCCCTATTACATCTCAATGTTTAAATGTGAAGCCTACTATTTCTGGAAAGCAAAACCCTCCTGCATGTTCTTCTCCTGCAGCTCCTATCCTAGCTTAA

Protein sequence

MHPEIVSIVMEFTLVRSLGINVINTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGKYNAEEPVEGIIVKNCTLMNTSNGVRIKSWPASPVAGTASDMHYEDIIMVNVSNPILIDQEYCPYNQCNKKSPSKVKISKVTFKNIRGTSGNALAIKLVCSSQVPCEGVELADIDLTYNGSEGPITSQCLNVKPTISGKQNPPACSSPAAPILA

Homology

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BLAST of Spg021447 vs. NCBI nr
Match: XP_022157387.1 (exopolygalacturonase-like [Momordica charantia] >XP_022159278.1 exopolygalacturonase-like [Momordica charantia])

HSP 1 Score: 357.8 bits (917), Expect = 6.4e-95
Identity = 169/198 (85.35%), Postives = 186/198 (93.94%), Query Frame = 0


Query: 16  RSLGINVINTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGKYNAEEPVEGIIV 75
           RS GIN+++TNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLG+Y AEEPVEGI  
Sbjct: 200 RSTGINILSTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGRYTAEEPVEGIRA 259

Query: 76  KNCTLMNTSNGVRIKSWPASPVAGTASDMHYEDIIMVNVSNPILIDQEYCPYNQCNKKSP 135
            NCT+MNTSNGVRIK+WPASP AGTASDMH+ED+IMVNVSNPILIDQEYCPYNQCNKKSP
Sbjct: 260 TNCTIMNTSNGVRIKTWPASPTAGTASDMHFEDMIMVNVSNPILIDQEYCPYNQCNKKSP 319

Query: 136 SKVKISKVTFKNIRGTSGNALAIKLVCSSQVPCEGVELADIDLTYNGSEGPITSQCLNVK 195
           SKVKISKV+FKN+RG+S NALA+KLVCS++VPCE VE+ADIDLTYNG+EGPI+SQC NVK
Sbjct: 320 SKVKISKVSFKNVRGSSANALAVKLVCSAEVPCEDVEVADIDLTYNGNEGPISSQCKNVK 379

Query: 196 PTISGKQNPPACSSPAAP 214
           P ISGKQNP ACSSPA P
Sbjct: 380 PIISGKQNPRACSSPAPP 397

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BLAST of Spg021447 vs. NCBI nr
Match: XP_008459709.2 (PREDICTED: exopolygalacturonase-like [Cucumis melo])

HSP 1 Score: 355.1 bits (910), Expect = 4.2e-94
Identity = 166/198 (83.84%), Postives = 183/198 (92.42%), Query Frame = 0


Query: 16  RSLGINVINTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGKYNAEEPVEGIIV 75
           RSLGIN++NTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLG+YN EEPVEG+ V
Sbjct: 195 RSLGINILNTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGRYNLEEPVEGLQV 254

Query: 76  KNCTLMNTSNGVRIKSWPASPVAGTASDMHYEDIIMVNVSNPILIDQEYCPYNQCNKKSP 135
           KNCTL  T+NG+RIK+WPASP AG ASDMHYED++M NV NPILIDQEYCPYNQCNK+ P
Sbjct: 255 KNCTLTGTTNGLRIKTWPASPAAGVASDMHYEDVVMNNVMNPILIDQEYCPYNQCNKQVP 314

Query: 136 SKVKISKVTFKNIRGTSGNALAIKLVCSSQVPCEGVELADIDLTYNGSEGPITSQCLNVK 195
           SKVKISKV+FKNIRGTSGNA A+ LVCSS  PC+GVELADIDLTY+GSEGPITS+C NVK
Sbjct: 315 SKVKISKVSFKNIRGTSGNAAAVTLVCSSSNPCDGVELADIDLTYSGSEGPITSRCANVK 374

Query: 196 PTISGKQNPPACSSPAAP 214
           PT+SGKQNPPAC+SPA P
Sbjct: 375 PTVSGKQNPPACTSPATP 392

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BLAST of Spg021447 vs. NCBI nr
Match: KAA0039364.1 (exopolygalacturonase-like [Cucumis melo var. makuwa] >TYK00547.1 exopolygalacturonase-like [Cucumis melo var. makuwa])

HSP 1 Score: 355.1 bits (910), Expect = 4.2e-94
Identity = 166/198 (83.84%), Postives = 183/198 (92.42%), Query Frame = 0


Query: 16  RSLGINVINTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGKYNAEEPVEGIIV 75
           RSLGIN++NTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLG+YN EEPVEG+ V
Sbjct: 247 RSLGINILNTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGRYNLEEPVEGLQV 306

Query: 76  KNCTLMNTSNGVRIKSWPASPVAGTASDMHYEDIIMVNVSNPILIDQEYCPYNQCNKKSP 135
           KNCTL  T+NG+RIK+WPASP AG ASDMHYED++M NV NPILIDQEYCPYNQCNK+ P
Sbjct: 307 KNCTLTGTTNGLRIKTWPASPAAGVASDMHYEDVVMNNVMNPILIDQEYCPYNQCNKQVP 366

Query: 136 SKVKISKVTFKNIRGTSGNALAIKLVCSSQVPCEGVELADIDLTYNGSEGPITSQCLNVK 195
           SKVKISKV+FKNIRGTSGNA A+ LVCSS  PC+GVELADIDLTY+GSEGPITS+C NVK
Sbjct: 367 SKVKISKVSFKNIRGTSGNAAAVTLVCSSSNPCDGVELADIDLTYSGSEGPITSRCANVK 426

Query: 196 PTISGKQNPPACSSPAAP 214
           PT+SGKQNPPAC+SPA P
Sbjct: 427 PTVSGKQNPPACTSPATP 444

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BLAST of Spg021447 vs. NCBI nr
Match: XP_022150984.1 (exopolygalacturonase-like [Momordica charantia])

HSP 1 Score: 345.9 bits (886), Expect = 2.5e-91
Identity = 166/199 (83.42%), Postives = 179/199 (89.95%), Query Frame = 0


Query: 16  RSLGINVINTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGKYNAEEPVEGIIV 75
           RS  +NV++T IGTGDDCISLGDGSK VTI NVTCGPGHGISVGSLG+Y  EEPVEGIIV
Sbjct: 204 RSNEVNVLSTRIGTGDDCISLGDGSKHVTINNVTCGPGHGISVGSLGRYTVEEPVEGIIV 263

Query: 76  KNCTLMNTSNGVRIKSWPASPVAGTASDMHYEDIIMVNVSNPILIDQEYCPYNQCNKKSP 135
           KNCT  NT NGVRIK+WPASP  GTA++MHYEDIIMVNVSNPILIDQEYCPYNQCNKK P
Sbjct: 264 KNCTFTNTDNGVRIKTWPASPTPGTATNMHYEDIIMVNVSNPILIDQEYCPYNQCNKKIP 323

Query: 136 SKVKISKVTFKNIRGTSGNALAIKLVCSSQVPCEGVELADIDLTYNGSEGPITSQCLNVK 195
           SKVKISKV+FKNIRG+S NALA+K++CSS  PCEGVE+ADIDLTYNGSEGPITSQC NVK
Sbjct: 324 SKVKISKVSFKNIRGSSANALAVKIICSSDFPCEGVEIADIDLTYNGSEGPITSQCSNVK 383

Query: 196 PTISGKQNPPACSSPAAPI 215
           P ISGKQNPPACSS  API
Sbjct: 384 PIISGKQNPPACSS-VAPI 401

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BLAST of Spg021447 vs. NCBI nr
Match: KAE8648971.1 (hypothetical protein Csa_008488 [Cucumis sativus])

HSP 1 Score: 340.1 bits (871), Expect = 1.4e-89
Identity = 162/200 (81.00%), Postives = 181/200 (90.50%), Query Frame = 0


Query: 14  LVRSLGINVINTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGKYNAEEPVEGI 73
           L R +GIN++N+NIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLG+YNAEEPVEG+
Sbjct: 57  LGRCVGINILNSNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGRYNAEEPVEGL 116

Query: 74  IVKNCTLMNTSNGVRIKSWPASPVAGTASDMHYEDIIMVNVSNPILIDQEYCPYNQCNKK 133
            VKNCT+  T++G+RIK+WPASP AG ASDMHYEDI+M NVSNPI+IDQEYCPYN+CNK+
Sbjct: 117 QVKNCTITGTTSGLRIKTWPASPAAGVASDMHYEDIVMNNVSNPIMIDQEYCPYNKCNKQ 176

Query: 134 SPSKVKISKVTFKNIRGTSGNALAIKLVCSSQVPCEGVELADIDLTYNGSEGPITSQCLN 193
            PSKVKISKV+FKNIRGTSGNA A+ LVCSS  PCEGVELADIDLTY G+EGPITS C N
Sbjct: 177 IPSKVKISKVSFKNIRGTSGNAQAVTLVCSSSNPCEGVELADIDLTYTGNEGPITSVCAN 236

Query: 194 VKPTISGKQNPPACSSPAAP 214
           VKPTI+GKQNPP C SP AP
Sbjct: 237 VKPTITGKQNPPIC-SPLAP 255

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BLAST of Spg021447 vs. ExPASy Swiss-Prot
Match: P24548 (Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 6.1e-72
Identity = 131/197 (66.50%), Postives = 151/197 (76.65%), Query Frame = 0


Query: 16  RSLGINVINTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGKYNAEEPVEGIIV 75
           RS G+N+INT I TGDDCISLGDGSK + ITN+TCGPGHGISVGSLG+Y  EE V GI V
Sbjct: 164 RSDGVNIINTEIKTGDDCISLGDGSKNINITNITCGPGHGISVGSLGRYKNEESVVGIYV 223

Query: 76  KNCTLMNTSNGVRIKSWPASPVAGTASDMHYEDIIMVNVSNPILIDQEYCPYNQCNKKSP 135
           KNCT+  + NGVRIK+WP S   G AS+MH++DI M +V  PILIDQ YCPYNQC  + P
Sbjct: 224 KNCTITGSQNGVRIKTWPKSE-PGEASEMHFQDITMNSVGTPILIDQGYCPYNQCTAEVP 283

Query: 136 SKVKISKVTFKNIRGTSGNALAIKLVCSSQVPCEGVELADIDLTYNGSEGPITSQCLNVK 195
           S VK+SK++FKNI+GTS    A+KLVCS   PC GVELADIDLTY+G  GP TS C N+K
Sbjct: 284 SSVKLSKISFKNIKGTSTTKEAVKLVCSKSFPCNGVELADIDLTYSGKGGPATSVCENIK 343

Query: 196 PTISGKQNPPACSSPAA 213
           PTI GKQ P  CS  AA
Sbjct: 344 PTIKGKQIPAICSGSAA 359

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BLAST of Spg021447 vs. ExPASy Swiss-Prot
Match: Q40312 (Polygalacturonase OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 1.5e-70
Identity = 120/198 (60.61%), Postives = 157/198 (79.29%), Query Frame = 0


Query: 16  RSLGINVINTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGKYNAEEPVEGIIV 75
           +S  + ++NTNIGTGDDC+S+GDGSKQ+T+  V CGPGHG+SVGSLGK+  EE VEGI V
Sbjct: 204 KSTDVKILNTNIGTGDDCVSIGDGSKQITVQGVNCGPGHGLSVGSLGKFTTEENVEGITV 263

Query: 76  KNCTLMNTSNGVRIKSWPASPVAGTASDMHYEDIIMVNVSNPILIDQEYCPYNQCNKKSP 135
           KNCTL  T NGVRIK+WP +P   T SD+H+EDI M NV NP++IDQEY P+NQC+KK+P
Sbjct: 264 KNCTLTATDNGVRIKTWPDAPGTITVSDIHFEDITMTNVKNPVIIDQEYYPWNQCSKKNP 323

Query: 136 SKVKISKVTFKNIRGTSGNALAIKLVCSSQVPCEGVELADIDLTYNGSEGPITSQCLNVK 195
           SK+K+SK++FKN++GTSG A  + L+CSS VPC+GVEL ++DL +NG+  P T++C NVK
Sbjct: 324 SKIKLSKISFKNVKGTSGTAEGVVLICSSAVPCDGVELNNVDLKFNGA--PTTAKCTNVK 383

Query: 196 PTISGKQNPPACSSPAAP 214
           P ++G    P C +P AP
Sbjct: 384 PLVTG--TAPVCQAPGAP 397

[Close]

BLAST of Spg021447 vs. ExPASy Swiss-Prot
Match: Q05967 (Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 9.1e-68
Identity = 119/197 (60.41%), Postives = 154/197 (78.17%), Query Frame = 0


Query: 16  RSLGINVINTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGKYNAEEPVEGIIV 75
           RS  +N+ ++N  TGDDCIS+GD ++Q+ IT VTCGPGHGISVGSLG    E+PV G+ V
Sbjct: 198 RSSSVNITDSNFSTGDDCISVGDETEQLYITRVTCGPGHGISVGSLGGNPDEKPVVGVFV 257

Query: 76  KNCTLMNTSNGVRIKSWPASPVAGTASDMHYEDIIMVNVSNPILIDQEYCPYNQCNKKSP 135
           +NCT  NT NGVRIK+WPAS   G  +D+H+EDII+ NVSNP++IDQ YCP+N+CNK  P
Sbjct: 258 RNCTFTNTDNGVRIKTWPASH-PGVVNDVHFEDIIVQNVSNPVVIDQVYCPFNKCNKDLP 317

Query: 136 SKVKISKVTFKNIRGTSGNALAIKLVCSSQVPCEGVELADIDLTYNGSEGPITSQCLNVK 195
           S+VKISKV+F+NI+GTS    A+ L+ S  VPCEG+E+ DID+TY+G EGP  S C N+K
Sbjct: 318 SQVKISKVSFQNIKGTSRTQDAVSLLRSKGVPCEGIEVGDIDITYSGKEGPAKSSCENIK 377

Query: 196 PTISGKQNPPACSSPAA 213
           P++ GKQNPP C++ AA
Sbjct: 378 PSLKGKQNPPVCTASAA 393

[Close]

BLAST of Spg021447 vs. ExPASy Swiss-Prot
Match: Q39786 (Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 1.2e-67
Identity = 121/195 (62.05%), Postives = 150/195 (76.92%), Query Frame = 0


Query: 16  RSLGINVINTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGKYNAEEPVEGIIV 75
           +S G+N+I ++I TGDDCIS+GDG+K + I  +TCGPGHGIS+GSLGK+  EEPVEGI +
Sbjct: 206 KSEGVNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIKI 265

Query: 76  KNCTLMNTSNGVRIKSWPASPVAGTASDMHYEDIIMVNVSNPILIDQEYCPYNQCNKKSP 135
            NCT+ NTSNG RIK+WP     G  S++H+EDI M NVS+PILIDQ+YCP+N+C K   
Sbjct: 266 SNCTITNTSNGARIKTWPGEH-GGAVSEIHFEDITMNNVSSPILIDQQYCPWNKCKKNEE 325

Query: 136 SKVKISKVTFKNIRGTSGNALAIKLVCSSQVPCEGVELADIDLTYNGSEGPITSQCLNVK 195
           SKVK+S ++FKNIRGTS    AIK +CS   PC+ VELADID+ +NG+E P TSQCLNVK
Sbjct: 326 SKVKLSNISFKNIRGTSALPEAIKFICSGSSPCQNVELADIDIKHNGAE-PATSQCLNVK 385

Query: 196 PTISGKQNPPACSSP 211
           P  SGK NP  CS P
Sbjct: 386 PITSGKLNPIPCSGP 398

[Close]

BLAST of Spg021447 vs. ExPASy Swiss-Prot
Match: W8P8Q3 (Probable galacturan 1,4-alpha-galacturonidase SALK6 OS=Kali turgidum OX=151250 PE=1 SV=3)

HSP 1 Score: 256.1 bits (653), Expect = 3.4e-67
Identity = 119/198 (60.10%), Postives = 152/198 (76.77%), Query Frame = 0


Query: 16  RSLGINVINTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGKYNAEEPVEGIIV 75
           RS G++  N+ IGTGDDCIS+GDG+  V +  +TCGPGHGIS+GS+GK+  E P  GI V
Sbjct: 204 RSNGVHATNSKIGTGDDCISMGDGAVDVHVEGITCGPGHGISIGSMGKFANEAPNTGIFV 263

Query: 76  KNCTLMNTSNGVRIKSWPASPVAGTASDMHYEDIIMVNVSNPILIDQEYCPYNQCNKKSP 135
           KNC+  +T NGVRIKSW  S    +ASD+H+EDI + NV NP++IDQEYCPYN C +K+P
Sbjct: 264 KNCSFTDTDNGVRIKSWMNS-FEASASDLHFEDITVTNVLNPVIIDQEYCPYNHCKEKTP 323

Query: 136 SKVKISKVTFKNIRGTSGNALAIKLVCSSQVPCEGVELADIDLTYNGSEGPITSQCLNVK 195
           SKVK+SK++FKN+ G + +A  +KL+CSS VPC+GVELADIDLT+ G  G   SQC NVK
Sbjct: 324 SKVKLSKISFKNVHGAAKSAEVVKLLCSSAVPCDGVELADIDLTFPG--GAAVSQCKNVK 383

Query: 196 PTISGKQNPPACSSPAAP 214
           P ++GKQNP AC +PA P
Sbjct: 384 PIVTGKQNPVACGAPATP 398

[Close]

BLAST of Spg021447 vs. ExPASy TrEMBL
Match: A0A6J1DUC6 (exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111024096 PE=3 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 3.1e-95
Identity = 169/198 (85.35%), Postives = 186/198 (93.94%), Query Frame = 0


Query: 16  RSLGINVINTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGKYNAEEPVEGIIV 75
           RS GIN+++TNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLG+Y AEEPVEGI  
Sbjct: 200 RSTGINILSTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGRYTAEEPVEGIRA 259

Query: 76  KNCTLMNTSNGVRIKSWPASPVAGTASDMHYEDIIMVNVSNPILIDQEYCPYNQCNKKSP 135
            NCT+MNTSNGVRIK+WPASP AGTASDMH+ED+IMVNVSNPILIDQEYCPYNQCNKKSP
Sbjct: 260 TNCTIMNTSNGVRIKTWPASPTAGTASDMHFEDMIMVNVSNPILIDQEYCPYNQCNKKSP 319

Query: 136 SKVKISKVTFKNIRGTSGNALAIKLVCSSQVPCEGVELADIDLTYNGSEGPITSQCLNVK 195
           SKVKISKV+FKN+RG+S NALA+KLVCS++VPCE VE+ADIDLTYNG+EGPI+SQC NVK
Sbjct: 320 SKVKISKVSFKNVRGSSANALAVKLVCSAEVPCEDVEVADIDLTYNGNEGPISSQCKNVK 379

Query: 196 PTISGKQNPPACSSPAAP 214
           P ISGKQNP ACSSPA P
Sbjct: 380 PIISGKQNPRACSSPAPP 397

[Close]

BLAST of Spg021447 vs. ExPASy TrEMBL
Match: A0A5A7T7B3 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001590 PE=3 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 2.0e-94
Identity = 166/198 (83.84%), Postives = 183/198 (92.42%), Query Frame = 0


Query: 16  RSLGINVINTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGKYNAEEPVEGIIV 75
           RSLGIN++NTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLG+YN EEPVEG+ V
Sbjct: 247 RSLGINILNTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGRYNLEEPVEGLQV 306

Query: 76  KNCTLMNTSNGVRIKSWPASPVAGTASDMHYEDIIMVNVSNPILIDQEYCPYNQCNKKSP 135
           KNCTL  T+NG+RIK+WPASP AG ASDMHYED++M NV NPILIDQEYCPYNQCNK+ P
Sbjct: 307 KNCTLTGTTNGLRIKTWPASPAAGVASDMHYEDVVMNNVMNPILIDQEYCPYNQCNKQVP 366

Query: 136 SKVKISKVTFKNIRGTSGNALAIKLVCSSQVPCEGVELADIDLTYNGSEGPITSQCLNVK 195
           SKVKISKV+FKNIRGTSGNA A+ LVCSS  PC+GVELADIDLTY+GSEGPITS+C NVK
Sbjct: 367 SKVKISKVSFKNIRGTSGNAAAVTLVCSSSNPCDGVELADIDLTYSGSEGPITSRCANVK 426

Query: 196 PTISGKQNPPACSSPAAP 214
           PT+SGKQNPPAC+SPA P
Sbjct: 427 PTVSGKQNPPACTSPATP 444

[Close]

BLAST of Spg021447 vs. ExPASy TrEMBL
Match: A0A1S3CC08 (exopolygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103498752 PE=3 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 2.0e-94
Identity = 166/198 (83.84%), Postives = 183/198 (92.42%), Query Frame = 0


Query: 16  RSLGINVINTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGKYNAEEPVEGIIV 75
           RSLGIN++NTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLG+YN EEPVEG+ V
Sbjct: 195 RSLGINILNTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGRYNLEEPVEGLQV 254

Query: 76  KNCTLMNTSNGVRIKSWPASPVAGTASDMHYEDIIMVNVSNPILIDQEYCPYNQCNKKSP 135
           KNCTL  T+NG+RIK+WPASP AG ASDMHYED++M NV NPILIDQEYCPYNQCNK+ P
Sbjct: 255 KNCTLTGTTNGLRIKTWPASPAAGVASDMHYEDVVMNNVMNPILIDQEYCPYNQCNKQVP 314

Query: 136 SKVKISKVTFKNIRGTSGNALAIKLVCSSQVPCEGVELADIDLTYNGSEGPITSQCLNVK 195
           SKVKISKV+FKNIRGTSGNA A+ LVCSS  PC+GVELADIDLTY+GSEGPITS+C NVK
Sbjct: 315 SKVKISKVSFKNIRGTSGNAAAVTLVCSSSNPCDGVELADIDLTYSGSEGPITSRCANVK 374

Query: 196 PTISGKQNPPACSSPAAP 214
           PT+SGKQNPPAC+SPA P
Sbjct: 375 PTVSGKQNPPACTSPATP 392

[Close]

BLAST of Spg021447 vs. ExPASy TrEMBL
Match: A0A6J1DBN7 (exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111019009 PE=3 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 1.2e-91
Identity = 166/199 (83.42%), Postives = 179/199 (89.95%), Query Frame = 0


Query: 16  RSLGINVINTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGKYNAEEPVEGIIV 75
           RS  +NV++T IGTGDDCISLGDGSK VTI NVTCGPGHGISVGSLG+Y  EEPVEGIIV
Sbjct: 204 RSNEVNVLSTRIGTGDDCISLGDGSKHVTINNVTCGPGHGISVGSLGRYTVEEPVEGIIV 263

Query: 76  KNCTLMNTSNGVRIKSWPASPVAGTASDMHYEDIIMVNVSNPILIDQEYCPYNQCNKKSP 135
           KNCT  NT NGVRIK+WPASP  GTA++MHYEDIIMVNVSNPILIDQEYCPYNQCNKK P
Sbjct: 264 KNCTFTNTDNGVRIKTWPASPTPGTATNMHYEDIIMVNVSNPILIDQEYCPYNQCNKKIP 323

Query: 136 SKVKISKVTFKNIRGTSGNALAIKLVCSSQVPCEGVELADIDLTYNGSEGPITSQCLNVK 195
           SKVKISKV+FKNIRG+S NALA+K++CSS  PCEGVE+ADIDLTYNGSEGPITSQC NVK
Sbjct: 324 SKVKISKVSFKNIRGSSANALAVKIICSSDFPCEGVEIADIDLTYNGSEGPITSQCSNVK 383

Query: 196 PTISGKQNPPACSSPAAPI 215
           P ISGKQNPPACSS  API
Sbjct: 384 PIISGKQNPPACSS-VAPI 401

[Close]

BLAST of Spg021447 vs. ExPASy TrEMBL
Match: A0A6J1E198 (exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111025503 PE=3 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 5.1e-82
Identity = 143/197 (72.59%), Postives = 169/197 (85.79%), Query Frame = 0


Query: 16  RSLGINVINTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGKYNAEEPVEGIIV 75
           RS GIN+I++NIGTGDDC+S+GD SKQ+T+T+VTCGPGHGIS+GSLGK+  EE VEG+ V
Sbjct: 212 RSTGINIISSNIGTGDDCVSIGDTSKQITVTDVTCGPGHGISIGSLGKFEGEENVEGVTV 271

Query: 76  KNCTLMNTSNGVRIKSWPASPVAGTASDMHYEDIIMVNVSNPILIDQEYCPYNQCNKKSP 135
           K C L+NTSNGVRIK+WP +P  G+ +DMH+EDI MVNVSNP+ IDQEYCP+N C K SP
Sbjct: 272 KKCKLINTSNGVRIKTWPGTPGTGSVTDMHFEDIEMVNVSNPVYIDQEYCPHNHCKKTSP 331

Query: 136 SKVKISKVTFKNIRGTSGNALAIKLVCSSQVPCEGVELADIDLTYNGSEGPITSQCLNVK 195
           SK+KISKVT KNI+GTS   +A+KLVCS   PCEGVE+ADIDLTYNG EGPI S+CLNVK
Sbjct: 332 SKIKISKVTIKNIKGTSATPVAVKLVCSQSHPCEGVEVADIDLTYNGKEGPIMSECLNVK 391

Query: 196 PTISGKQNPPACSSPAA 213
           PTISGKQNP AC+ PAA
Sbjct: 392 PTISGKQNPRACAKPAA 408

[Close]

BLAST of Spg021447 vs. TAIR 10
Match: AT3G07820.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 263.5 bits (672), Expect = 1.5e-70
Identity = 121/199 (60.80%), Postives = 154/199 (77.39%), Query Frame = 0


Query: 14  LVRSLGINVINTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGKYNAEEPVEGI 73
           L RS G+ ++N+ I TGDDCIS+GDG K + +  VTCGPGHGISVGSLG+Y  E+ V GI
Sbjct: 193 LGRSDGVKILNSFISTGDDCISVGDGMKNLHVEKVTCGPGHGISVGSLGRYGHEQDVSGI 252

Query: 74  IVKNCTLMNTSNGVRIKSWPASPVAGTASDMHYEDIIMVNVSNPILIDQEYCPYNQCNKK 133
            V NCTL  T NG+RIK+WP++  + TASD+H+EDII+ +VSNPILIDQEYCP+NQCNK+
Sbjct: 253 KVINCTLQETDNGLRIKTWPSAACSTTASDIHFEDIILKDVSNPILIDQEYCPWNQCNKQ 312

Query: 134 SPSKVKISKVTFKNIRGTSGNALAIKLVCSSQVPCEGVELADIDLTYNGSEGPITSQCLN 193
             S +K+  ++FKNIRGTSGN  A+KL+CS   PC+ VE+ DID+ YNG++GP T  C N
Sbjct: 313 KASTIKLVNISFKNIRGTSGNKDAVKLLCSKGYPCQNVEIGDIDIKYNGADGPATFHCSN 372

Query: 194 VKPTISGKQNPPACSSPAA 213
           V P I G Q+P ACS+PAA
Sbjct: 373 VSPKILGSQSPKACSAPAA 391

[Close]

BLAST of Spg021447 vs. TAIR 10
Match: AT5G48140.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 258.1 bits (658), Expect = 6.4e-69
Identity = 115/195 (58.97%), Postives = 151/195 (77.44%), Query Frame = 0


Query: 16  RSLGINVINTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGKYNAEEPVEGIIV 75
           RS GI +IN+ I TGDDC+S+GDG K + +  VTCGPGHGIS+GSLG+Y+ EE V GI +
Sbjct: 195 RSDGIKIINSFISTGDDCVSVGDGMKNLLVERVTCGPGHGISIGSLGRYSHEENVSGIKI 254

Query: 76  KNCTLMNTSNGVRIKSWPASPVAGTASDMHYEDIIMVNVSNPILIDQEYCPYNQCNKKSP 135
            NCTL  T NG+RIK+WP++    TASD+H+E+I++ NVSNPILIDQEYCP+NQCNK+ P
Sbjct: 255 INCTLQETDNGLRIKTWPSAACTTTASDIHFENILLKNVSNPILIDQEYCPWNQCNKQKP 314

Query: 136 SKVKISKVTFKNIRGTSGNALAIKLVCSSQVPCEGVELADIDLTYNGSEGPITSQCLNVK 195
           S +K++ ++FK IRGTSGN  A+KL+CS   PC+ VE+ D+++ Y G++GP T QC NV 
Sbjct: 315 STIKLANISFKKIRGTSGNKDAVKLLCSKGYPCQNVEVGDVNIQYTGADGPATFQCSNVS 374

Query: 196 PTISGKQNPPACSSP 211
           P + G Q P ACSSP
Sbjct: 375 PKLVGTQIPKACSSP 389

[Close]

BLAST of Spg021447 vs. TAIR 10
Match: AT3G07840.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 253.4 bits (646), Expect = 1.6e-67
Identity = 118/200 (59.00%), Postives = 150/200 (75.00%), Query Frame = 0


Query: 14  LVRSLGINVINTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGKYNAEEPVEGI 73
           L RS G+ ++N+ I TGDDCIS+GDG K + + NV CGPGHGISVGSLG+Y  E+ V GI
Sbjct: 194 LGRSEGVKILNSKIATGDDCISVGDGMKNLHVENVMCGPGHGISVGSLGRYVHEQDVTGI 253

Query: 74  IVKNCTLMNTSNGVRIKSWPASPVAGTASDMHYEDIIMVNVSNPILIDQEYCPYNQCNKK 133
            V NCTL  T NG+RIK+WP++  A TAS +H+E+II+ NVSNPILIDQEYCP+NQCNK+
Sbjct: 254 TVVNCTLQGTDNGLRIKTWPSAACATTASGIHFENIILNNVSNPILIDQEYCPWNQCNKQ 313

Query: 134 SPSKVKISKVTFKNIRGTSGNALAIKLVCSSQVPCEGVELADIDLTYNGSEGPITSQCLN 193
            PS +K+  ++FKNIRGTSGN  A+KL+CS   PC  VE+ +I+L Y G++GP T  C N
Sbjct: 314 KPSTIKLVDISFKNIRGTSGNKDAVKLLCSKAHPCANVEIGNINLEYKGADGPPTFMCSN 373

Query: 194 VKPTISGKQNPPACSSPAAP 214
           V P + G QNP ACS+   P
Sbjct: 374 VSPKLVGTQNPKACSAGPTP 393

[Close]

BLAST of Spg021447 vs. TAIR 10
Match: AT3G07830.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 250.8 bits (639), Expect = 1.0e-66
Identity = 113/202 (55.94%), Postives = 155/202 (76.73%), Query Frame = 0


Query: 14  LVRSLGINVINTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGKYNAEEPVEGI 73
           L RS+G+++IN+ I TGDDC+S+GDG   + + NV CGPGHGISVGSLG+Y  E+ V GI
Sbjct: 194 LGRSVGVSIINSRISTGDDCVSVGDGMVNLLVKNVVCGPGHGISVGSLGRYGHEQDVSGI 253

Query: 74  IVKNCTLMNTSNGVRIKSWPASPVAGTASDMHYEDIIMVNVSNPILIDQEYCPYNQCNKK 133
            V NCTL  T NG+RIK+WP++  + TAS++H+E+II+ NVSNPILIDQEYCP+NQCNK+
Sbjct: 254 RVINCTLQETDNGLRIKTWPSAACSTTASNIHFENIILRNVSNPILIDQEYCPWNQCNKQ 313

Query: 134 SPSKVKISKVTFKNIRGTSGNALAIKLVCSSQVPCEGVELADIDLTYNGSEGPITSQCLN 193
             S +K++ ++F+ IRGTSGN  A+KL+CS   PCE V++ DI++ Y G++GP T  C N
Sbjct: 314 KSSSIKLANISFRRIRGTSGNKDAVKLLCSKGYPCENVQVGDINIQYTGADGPATFMCSN 373

Query: 194 VKPTISGKQNPPACSSPAAPIL 216
           V+P + G Q P AC++P  P+L
Sbjct: 374 VRPKLVGTQFPKACNTP--PVL 393

[Close]

BLAST of Spg021447 vs. TAIR 10
Match: AT3G14040.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 243.8 bits (621), Expect = 1.3e-64
Identity = 113/198 (57.07%), Postives = 145/198 (73.23%), Query Frame = 0


Query: 16  RSLGINVINTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGKYNAEEPVEGIIV 75
           RS G+N+I   I TGDDC+S+GDG++ + + NV CGPGHGIS+GSLG+Y  E+PV+G+ V
Sbjct: 247 RSNGVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTV 306

Query: 76  KNCTLMNTSNGVRIKSWPASPVAGTASDMHYEDIIMVNVSNPILIDQEYCPYNQCNKKSP 135
           + C + NT NGVRIK+WP SP  G AS++ +EDI M NVS P+LIDQEYCPY  C    P
Sbjct: 307 RKCLIKNTDNGVRIKTWPGSP-PGIASNILFEDITMDNVSLPVLIDQEYCPYGHCKAGVP 366

Query: 136 SKVKISKVTFKNIRGTSGNALAIKLVCSSQVPCEGVELADIDLTYNGSEGPITSQCLNVK 195
           SKVK+S VT KNI+GTS   +A+KL+CS  VPC  + L+DI+L +NG EGP  S C N+K
Sbjct: 367 SKVKLSDVTIKNIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNGKEGPAVSACSNIK 426

Query: 196 PTISGKQNPPACSSPAAP 214
           P +SGK  P AC+  A P
Sbjct: 427 PILSGKLVPAACTEVAKP 443

The following BLAST results are available for this feature:

BLAST of Spg021447 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022157387.1	6.4e-95	85.35	exopolygalacturonase-like [Momordica charantia] >XP_022159278.1 exopolygalacturo...	[more]
XP_008459709.2	4.2e-94	83.84	PREDICTED: exopolygalacturonase-like [Cucumis melo]	[more]
KAA0039364.1	4.2e-94	83.84	exopolygalacturonase-like [Cucumis melo var. makuwa] >TYK00547.1 exopolygalactur...	[more]
XP_022150984.1	2.5e-91	83.42	exopolygalacturonase-like [Momordica charantia]	[more]
KAE8648971.1	1.4e-89	81.00	hypothetical protein Csa_008488 [Cucumis sativus]	[more]

BLAST of Spg021447 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P24548	6.1e-72	66.50	Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1	[more]
Q40312	1.5e-70	60.61	Polygalacturonase OS=Medicago sativa OX=3879 PE=2 SV=1	[more]
Q05967	9.1e-68	60.41	Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1	[more]
Q39786	1.2e-67	62.05	Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1	[more]
W8P8Q3	3.4e-67	60.10	Probable galacturan 1,4-alpha-galacturonidase SALK6 OS=Kali turgidum OX=151250 P...	[more]

BLAST of Spg021447 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DUC6	3.1e-95	85.35	exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111024096 PE=3 SV...	[more]
A0A5A7T7B3	2.0e-94	83.84	Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S3CC08	2.0e-94	83.84	exopolygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103498752 PE=3 SV=1	[more]
A0A6J1DBN7	1.2e-91	83.42	exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111019009 PE=3 SV...	[more]
A0A6J1E198	5.1e-82	72.59	exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111025503 PE=3 SV...	[more]

BLAST of Spg021447 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G07820.1	1.5e-70	60.80	Pectin lyase-like superfamily protein	[more]
AT5G48140.1	6.4e-69	58.97	Pectin lyase-like superfamily protein	[more]
AT3G07840.1	1.6e-67	59.00	Pectin lyase-like superfamily protein	[more]
AT3G07830.1	1.0e-66	55.94	Pectin lyase-like superfamily protein	[more]
AT3G14040.1	1.3e-64	57.07	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 12..212 e-value: 6.5E-60 score: 205.2
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 13..197 e-value: 2.5E-62 score: 210.8
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 47..60
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 16..210
None	No IPR available	PANTHER	PTHR31375:SF248	POLYGALACTURONASE	coord: 16..210
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 14..208

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg021447.1	Spg021447.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity