Spg021438 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg021438
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionL-ascorbate oxidase homolog
Locationscaffold4: 355644 .. 358058 (+)
RNA-Seq ExpressionSpg021438
SyntenySpg021438
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGGAGACACAACCCATCTACTTACAATTGTGGGGAGATTTTGGGATACATAGCCTGCTCGATATAAAAGAGCAGTGATTTGATAAAAAGAGAAGAAAGGGGGAGATACAAATGGCTGGGTTGATGTTTACCACGTTGCTTTGCCTCTCGGCTGCAGCAATGTTAACAGTACAGGGTGAGGACCCTTACTTTTTCTTCACGTGGAACGTTACCTATGGCACCATCTCTCCCTTGGGTGTTCCCCAGCAAGGCATTCTCATCAACGGTCAATTCCCTGGACCTAATATCAACTCCACAACCAACAATAACATAGTCATTAATGTCTTCAATAATCTCGATGAACCCTTCCTTCTACATTGGTACAATAATTATATTTATATTAACATATTATACATGCATGTTATGTGTATATATAATCTTTCTTCTTCATTTAATTCTTTCAATACAAAAAATAAATGAAATTGGAATTGCAGGAGTGGTATTCAACATAGAAAGAACTCGTGGCAAGATGGACTACTTGGAACCAATTGCCCAATCCCACCTGGGACAAACTTCACCTACCGTTTCCAAGTAAAGGATCAAATTGGAAGTTTCTTCTACTACCCATCAACTGCCATGCATAGGGCTGGGGGAGCCTTTGGTGGTCTTCGTGTGAATAGTCGTTTACTCATCCCTGTCCCTTATGCTGACCCTGAAGATGATTACACTGTTCTTATTGGCGACTGGTACACCAAGAGCCACTCCACCCTTAAAAAGTTCTTAGACAGTGGCCGCTCCATCGCTAGACCTGATGGTGTTCTCATTAACGGGAAGGCAGCAAAGGGTGATGGAACCGATGAGCCACTCTTCACCATGAAGCCTGGGAAGACTTATAAGTACCGAATATGCAATGTTGGACTAAAGACATCTCTAAACTTCAGATTCCAAGGCCACATCATGAAATTGGTAGAGATGGAGGGCTCTCATACCGTCCAAAATGATTATCAATCACTTGATATCCATGTGGGACAGTGCTTGGCAGTGTTAGTCACAGCCAACCAAGACCCTAAGGACTATTATATGGTTGCATCCACTCGATTCATCAAGAATGTTCTTGTTGGTAAAGGCATTATTCGATACACCAATGGAAATGGCCTTGCCTCTCATGAAATTCCTGAGGCACCTATGGGTTGGGCTTGGTCCCTCAATCAATTTCGTACTTTTCGTTGGAACCTTACTGCCAGCGCTGCTAGGCCTAATCCTCAAGGCTCTTATCACTATGGTTCCATCAACATTACTCGAACCATCAAGATAGTTAATTCAGCTGCTAAGGTTGATGGTAAGCTGCGATATGCCATTAATGGTGTCTCTCATATTGACCCTGAAACTCCATTGAAGCTTGCAGAGTACTTTGGAGTTAGTGACAAGGTCTTCAAGTATGATACAATTTCTGATGAAGGACCTGCAGAAAGTGCCAGCACTGTGACTATAGCTCCTAATGTTGTTAATGCAACTTTTCGCAATTTTGTTGAGATTATATTTGAGAACCATGAGAAGAGCCTCCAATCGTGGCATTTCGATGGCTACTCCTTCTTTGCCGTTGCGTAAGTATCATGGACAAAAAATAATTAATGTATATATGTATGCACGTATATCTTTTCTTTTTTTTTTTTTTTTTTTTTGAAAAAAGCATATCTTTTCTACACCCAATCTACACATGACTAAGGAATTCTTTATTGATATTTCATACAGCATTGAGCCTGGGAGATGGACTCCAGAAAAACGAAAGAACTATAATCTTCTAGATGCTGTGAGCAGACATACAATCCAAGTCTTCCCTAAATCATGGGCAGCCATTCTTCTTACATTAGACAATGTTGGAATGTGGAACTTGAGATCTGAATTGACAGAAAATCGTTACTTGGGACAACAACTCTACATCAGTGTTCTTTCACCAGCTCGCTCCCTTAGAGATGAATACAACATCCCAGATAACACTTTGCTCTGCGGTTTGGTCAAAGACATGCCATTGCCCAAGCCGTATACCATTTGAGGCAATTTATCCATCCCTCCCCCATTTTAATTAATTAAGATACTATGATATTTATAGGGGTGATCATGAGTCTCCCAATAAGTTGCTACGATTCAGAAATTCATGAAAACCTCGTGATTGGAAAACCCTCAAAATATCCTTTAGATAACATAAAACAAGGAATAGGGAAGTTTATTACTACTCTCTCTCTATATATAGTCTTCATGAAACGTCAAGAGTCTTTTGATATTAGTTTTTCCTTATGTTGCAATTGCTTTTCTGGCGCATATAGGTCATCTTCTACGCGATTTAAATTGGGAAAATTTTAATGAACTGACCGCGTGATGATTTTAAATAATCCTATACTAAGATCCCGAGTTTGCATGAATAAAAAGTGATTAGGGG

mRNA sequence

ATGGCTGGGTTGATGTTTACCACGTTGCTTTGCCTCTCGGCTGCAGCAATGTTAACAGTACAGGGTGAGGACCCTTACTTTTTCTTCACGTGGAACGTTACCTATGGCACCATCTCTCCCTTGGGTGTTCCCCAGCAAGGCATTCTCATCAACGGTCAATTCCCTGGACCTAATATCAACTCCACAACCAACAATAACATAGTCATTAATGTCTTCAATAATCTCGATGAACCCTTCCTTCTACATTGGAGTGGTATTCAACATAGAAAGAACTCGTGGCAAGATGGACTACTTGGAACCAATTGCCCAATCCCACCTGGGACAAACTTCACCTACCGTTTCCAAGTAAAGGATCAAATTGGAAGTTTCTTCTACTACCCATCAACTGCCATGCATAGGGCTGGGGGAGCCTTTGGTGGTCTTCGTGTGAATAGTCGTTTACTCATCCCTGTCCCTTATGCTGACCCTGAAGATGATTACACTGTTCTTATTGGCGACTGGTACACCAAGAGCCACTCCACCCTTAAAAAGTTCTTAGACAGTGGCCGCTCCATCGCTAGACCTGATGGTGTTCTCATTAACGGGAAGGCAGCAAAGGGTGATGGAACCGATGAGCCACTCTTCACCATGAAGCCTGGGAAGACTTATAAGTACCGAATATGCAATGTTGGACTAAAGACATCTCTAAACTTCAGATTCCAAGGCCACATCATGAAATTGGTAGAGATGGAGGGCTCTCATACCGTCCAAAATGATTATCAATCACTTGATATCCATGTGGGACAGTGCTTGGCAGTGTTAGTCACAGCCAACCAAGACCCTAAGGACTATTATATGGTTGCATCCACTCGATTCATCAAGAATGTTCTTGTTGGTAAAGGCATTATTCGATACACCAATGGAAATGGCCTTGCCTCTCATGAAATTCCTGAGGCACCTATGGGTTGGGCTTGGTCCCTCAATCAATTTCGTACTTTTCGTTGGAACCTTACTGCCAGCGCTGCTAGGCCTAATCCTCAAGGCTCTTATCACTATGGTTCCATCAACATTACTCGAACCATCAAGATAGTTAATTCAGCTGCTAAGGTTGATGGTAAGCTGCGATATGCCATTAATGGTGTCTCTCATATTGACCCTGAAACTCCATTGAAGCTTGCAGAGTACTTTGGAGTTAGTGACAAGGTCTTCAAGTATGATACAATTTCTGATGAAGGACCTGCAGAAAGTGCCAGCACTGTGACTATAGCTCCTAATGTTGTTAATGCAACTTTTCGCAATTTTGTTGAGATTATATTTGAGAACCATGAGAAGAGCCTCCAATCGTGGCATTTCGATGGCTACTCCTTCTTTGCCGTTGCCATTGAGCCTGGGAGATGGACTCCAGAAAAACGAAAGAACTATAATCTTCTAGATGCTGTGAGCAGACATACAATCCAAGTCTTCCCTAAATCATGGGCAGCCATTCTTCTTACATTAGACAATGTTGGAATGTGGAACTTGAGATCTGAATTGACAGAAAATCGTTACTTGGGACAACAACTCTACATCAGTGTTCTTTCACCAGCTCGCTCCCTTAGAGATGAATACAACATCCCAGATAACACTTTGCTCTGCGGTTTGGTCAAAGACATGCCATTGCCCAAGCCGTATACCATTTGA

Coding sequence (CDS)

ATGGCTGGGTTGATGTTTACCACGTTGCTTTGCCTCTCGGCTGCAGCAATGTTAACAGTACAGGGTGAGGACCCTTACTTTTTCTTCACGTGGAACGTTACCTATGGCACCATCTCTCCCTTGGGTGTTCCCCAGCAAGGCATTCTCATCAACGGTCAATTCCCTGGACCTAATATCAACTCCACAACCAACAATAACATAGTCATTAATGTCTTCAATAATCTCGATGAACCCTTCCTTCTACATTGGAGTGGTATTCAACATAGAAAGAACTCGTGGCAAGATGGACTACTTGGAACCAATTGCCCAATCCCACCTGGGACAAACTTCACCTACCGTTTCCAAGTAAAGGATCAAATTGGAAGTTTCTTCTACTACCCATCAACTGCCATGCATAGGGCTGGGGGAGCCTTTGGTGGTCTTCGTGTGAATAGTCGTTTACTCATCCCTGTCCCTTATGCTGACCCTGAAGATGATTACACTGTTCTTATTGGCGACTGGTACACCAAGAGCCACTCCACCCTTAAAAAGTTCTTAGACAGTGGCCGCTCCATCGCTAGACCTGATGGTGTTCTCATTAACGGGAAGGCAGCAAAGGGTGATGGAACCGATGAGCCACTCTTCACCATGAAGCCTGGGAAGACTTATAAGTACCGAATATGCAATGTTGGACTAAAGACATCTCTAAACTTCAGATTCCAAGGCCACATCATGAAATTGGTAGAGATGGAGGGCTCTCATACCGTCCAAAATGATTATCAATCACTTGATATCCATGTGGGACAGTGCTTGGCAGTGTTAGTCACAGCCAACCAAGACCCTAAGGACTATTATATGGTTGCATCCACTCGATTCATCAAGAATGTTCTTGTTGGTAAAGGCATTATTCGATACACCAATGGAAATGGCCTTGCCTCTCATGAAATTCCTGAGGCACCTATGGGTTGGGCTTGGTCCCTCAATCAATTTCGTACTTTTCGTTGGAACCTTACTGCCAGCGCTGCTAGGCCTAATCCTCAAGGCTCTTATCACTATGGTTCCATCAACATTACTCGAACCATCAAGATAGTTAATTCAGCTGCTAAGGTTGATGGTAAGCTGCGATATGCCATTAATGGTGTCTCTCATATTGACCCTGAAACTCCATTGAAGCTTGCAGAGTACTTTGGAGTTAGTGACAAGGTCTTCAAGTATGATACAATTTCTGATGAAGGACCTGCAGAAAGTGCCAGCACTGTGACTATAGCTCCTAATGTTGTTAATGCAACTTTTCGCAATTTTGTTGAGATTATATTTGAGAACCATGAGAAGAGCCTCCAATCGTGGCATTTCGATGGCTACTCCTTCTTTGCCGTTGCCATTGAGCCTGGGAGATGGACTCCAGAAAAACGAAAGAACTATAATCTTCTAGATGCTGTGAGCAGACATACAATCCAAGTCTTCCCTAAATCATGGGCAGCCATTCTTCTTACATTAGACAATGTTGGAATGTGGAACTTGAGATCTGAATTGACAGAAAATCGTTACTTGGGACAACAACTCTACATCAGTGTTCTTTCACCAGCTCGCTCCCTTAGAGATGAATACAACATCCCAGATAACACTTTGCTCTGCGGTTTGGTCAAAGACATGCCATTGCCCAAGCCGTATACCATTTGA

Protein sequence

MAGLMFTTLLCLSAAAMLTVQGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTTNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYRFQVKDQIGSFFYYPSTAMHRAGGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFLDSGRSIARPDGVLINGKAAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRFQGHIMKLVEMEGSHTVQNDYQSLDIHVGQCLAVLVTANQDPKDYYMVASTRFIKNVLVGKGIIRYTNGNGLASHEIPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVNSAAKVDGKLRYAINGVSHIDPETPLKLAEYFGVSDKVFKYDTISDEGPAESASTVTIAPNVVNATFRNFVEIIFENHEKSLQSWHFDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQVFPKSWAAILLTLDNVGMWNLRSELTENRYLGQQLYISVLSPARSLRDEYNIPDNTLLCGLVKDMPLPKPYTI
Homology
BLAST of Spg021438 vs. NCBI nr
Match: XP_022137060.1 (L-ascorbate oxidase homolog [Momordica charantia])

HSP 1 Score: 1083.6 bits (2801), Expect = 0.0e+00
Identity = 511/551 (92.74%), Postives = 529/551 (96.01%), Query Frame = 0

Query: 3   GLMFTTLLCLSAAAML-TVQGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINS 62
           GLMFT LLCL AAAM+ TV+GEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINS
Sbjct: 5   GLMFTMLLCLLAAAMMSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINS 64

Query: 63  TTNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYRFQVKDQIG 122
           TTNNN+V+NVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTY FQVKDQIG
Sbjct: 65  TTNNNLVVNVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQIG 124

Query: 123 SFFYYPSTAMHRAGGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFLDS 182
           S+FYYPSTAMHRA GAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWY KSHSTLK FLDS
Sbjct: 125 SYFYYPSTAMHRAAGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYXKSHSTLKNFLDS 184

Query: 183 GRSIARPDGVLINGKAAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRFQGHIMKLV 242
           GRS+ARPDGVLINGK AKGDGTDEPLFTMKPGKTYKYRICNVGLKT+LNFRFQGH MKLV
Sbjct: 185 GRSLARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRICNVGLKTTLNFRFQGHTMKLV 244

Query: 243 EMEGSHTVQNDYQSLDIHVGQCLAVLVTANQDPKDYYMVASTRFIKNVLVGKGIIRYTNG 302
           EMEGSH VQNDYQSLDIHVGQC +VLV ANQ+PKDYYMVASTRFIK++LVGKGI+RYTNG
Sbjct: 245 EMEGSHVVQNDYQSLDIHVGQCFSVLVRANQEPKDYYMVASTRFIKSLLVGKGIVRYTNG 304

Query: 303 NGLASHEIPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVNSAA 362
            G AS EIPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIK+VNSA 
Sbjct: 305 KGPASPEIPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNSAT 364

Query: 363 KVDGKLRYAINGVSHIDPETPLKLAEYFGVSDKVFKYDTISDEGPAESASTVTIAPNVVN 422
           K DGKL+YAINGVSHIDPETPLKLAEYFG++DKVFKYDTISDEGP ESA+TVTIAPNVVN
Sbjct: 365 KTDGKLQYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDEGPTESATTVTIAPNVVN 424

Query: 423 ATFRNFVEIIFENHEKSLQSWHFDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQVF 482
           ATFRNFVEIIFENHEKSLQSWHFDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQVF
Sbjct: 425 ATFRNFVEIIFENHEKSLQSWHFDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQVF 484

Query: 483 PKSWAAILLTLDNVGMWNLRSELTENRYLGQQLYISVLSPARSLRDEYNIPDNTLLCGLV 542
           PKSWAAILLTLDNVGMWNLRSELTENRYLGQQLYISV+SPA SLRDEYNIPDN LLCGLV
Sbjct: 485 PKSWAAILLTLDNVGMWNLRSELTENRYLGQQLYISVISPAHSLRDEYNIPDNALLCGLV 544

Query: 543 KDMPLPKPYTI 553
           K MPLP PYTI
Sbjct: 545 KGMPLPXPYTI 555

BLAST of Spg021438 vs. NCBI nr
Match: XP_022153749.1 (L-ascorbate oxidase homolog [Momordica charantia])

HSP 1 Score: 1081.2 bits (2795), Expect = 0.0e+00
Identity = 505/552 (91.49%), Postives = 528/552 (95.65%), Query Frame = 0

Query: 1   MAGLMFTTLLCLSAAAMLTVQGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNIN 60
           MAGLMFT LLCLSAAAM TV+GEDPYFFFTWN+TYGTISPLGVPQQGILINGQFPGPNIN
Sbjct: 1   MAGLMFTVLLCLSAAAMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNIN 60

Query: 61  STTNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYRFQVKDQI 120
           ST+NNNIV+NVFNNLDEPFL+HWSGIQHRKNSWQDGLLGTNCPIPPGTN+TY FQVKDQI
Sbjct: 61  STSNNNIVVNVFNNLDEPFLIHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI 120

Query: 121 GSFFYYPSTAMHRAGGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFLD 180
           GSFFYYPSTAMHRA G FGGLRVNSRLLIPVPYADPEDDYTVL+GDWYTKSHS LK FLD
Sbjct: 121 GSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLVGDWYTKSHSALKNFLD 180

Query: 181 SGRSIARPDGVLINGKAAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRFQGHIMKL 240
           SGRS+ARPDGVLINGK AKGDGTDEPLFTMKP KTYKYR+CN+GLKTSLN RFQ H MKL
Sbjct: 181 SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQDHTMKL 240

Query: 241 VEMEGSHTVQNDYQSLDIHVGQCLAVLVTANQDPKDYYMVASTRFIKNVLVGKGIIRYTN 300
           VEMEGSH VQNDYQSLDIHVGQC +VLVTANQ+PKDYY++ASTRF KNVLVGKGIIRYTN
Sbjct: 241 VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN 300

Query: 301 GNGLASHEIPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVNSA 360
           G G AS EIPEAP+GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKI+NSA
Sbjct: 301 GKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA 360

Query: 361 AKVDGKLRYAINGVSHIDPETPLKLAEYFGVSDKVFKYDTISDEGPAESASTVTIAPNVV 420
            KVDGKLRYAINGVSHIDPETPLKLAEYFG++DKVFKYDTISD+GPAE A+TVT+APNVV
Sbjct: 361 TKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVV 420

Query: 421 NATFRNFVEIIFENHEKSLQSWHFDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQV 480
           NATFRNFVEI+FENHEKSLQSWH +GYSFFAVAIEPGRWTPEKRKNYNLLDAVSR TIQV
Sbjct: 421 NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRDTIQV 480

Query: 481 FPKSWAAILLTLDNVGMWNLRSELTENRYLGQQLYISVLSPARSLRDEYNIPDNTLLCGL 540
           FPKSWAAILLTLDN GMWNLRSELTENRYLGQQLY SVLSPARSLRDEYNIPDNTLLCGL
Sbjct: 481 FPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL 540

Query: 541 VKDMPLPKPYTI 553
           VKDMPLPKPYTI
Sbjct: 541 VKDMPLPKPYTI 552

BLAST of Spg021438 vs. NCBI nr
Match: KAA0040130.1 (L-ascorbate oxidase-like protein [Cucumis melo var. makuwa] >TYK21751.1 L-ascorbate oxidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1081.2 bits (2795), Expect = 0.0e+00
Identity = 508/553 (91.86%), Postives = 531/553 (96.02%), Query Frame = 0

Query: 1   MAGLMFTTLLCLSAAAMLT-VQGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MAGLMFTTLLCLSAAAM T V+GEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MAGLMFTTLLCLSAAAMTTMVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTTNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYRFQVKDQ 120
           NSTTNNN+VINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTY FQVKDQ
Sbjct: 61  NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAGGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFL 180
           IGSFFYYPSTAMHRA G FGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSH+TLK+FL
Sbjct: 121 IGSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQFL 180

Query: 181 DSGRSIARPDGVLINGKAAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRFQGHIMK 240
           DSGRSIARPDGVLINGK AKGDGTDEPLFTMKPGKTYKYR+CNVGLKTSLNFRFQGH MK
Sbjct: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTMK 240

Query: 241 LVEMEGSHTVQNDYQSLDIHVGQCLAVLVTANQDPKDYYMVASTRFIKNVLVGKGIIRYT 300
           LVEMEGSHTVQNDY+SLD+HVGQC +VLVTA+Q+PKDYYMVASTRFIK+ LVGKGI+RYT
Sbjct: 241 LVEMEGSHTVQNDYKSLDVHVGQCFSVLVTADQEPKDYYMVASTRFIKSALVGKGIVRYT 300

Query: 301 NGNGLASHEIPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVNS 360
           NG G AS E+P APMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIK+VNS
Sbjct: 301 NGKGPASPELPNAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNS 360

Query: 361 AAKVDGKLRYAINGVSHIDPETPLKLAEYFGVSDKVFKYDTISDEGPAESASTVTIAPNV 420
           A+KVDGKLRYAINGVSH+DPETPLKLAEYFGV+DKVFKYDTISDEG AE A+TVT+APNV
Sbjct: 361 ASKVDGKLRYAINGVSHVDPETPLKLAEYFGVTDKVFKYDTISDEGLAEGATTVTVAPNV 420

Query: 421 VNATFRNFVEIIFENHEKSLQSWHFDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQ 480
           VN TFRNF+EIIFENHEKSLQSWH DGYSFFAVAIEPGRWTPEKR +YNLLDAVSRHTIQ
Sbjct: 421 VNTTFRNFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWTPEKRSDYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAILLTLDNVGMWNLRSELTENRYLGQQLYISVLSPARSLRDEYNIPDNTLLCG 540
           VFPKSWAAILLT DN GMWNLRSEL ENRYLGQQLY+SV SPARSLRDEYNIPDNTLLCG
Sbjct: 481 VFPKSWAAILLTFDNAGMWNLRSELAENRYLGQQLYMSVQSPARSLRDEYNIPDNTLLCG 540

Query: 541 LVKDMPLPKPYTI 553
           LVKD+PLPKPYTI
Sbjct: 541 LVKDLPLPKPYTI 553

BLAST of Spg021438 vs. NCBI nr
Match: KAA0040123.1 (L-ascorbate oxidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1080.5 bits (2793), Expect = 0.0e+00
Identity = 507/553 (91.68%), Postives = 533/553 (96.38%), Query Frame = 0

Query: 1   MAGLMFTTLLCLSAAAML-TVQGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MAGLMFT LLCLSAAAM+ TVQGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MAGLMFTALLCLSAAAMMATVQGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTTNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYRFQVKDQ 120
           NST+NNN+VINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPI PGTNFTY FQVKDQ
Sbjct: 61  NSTSNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIQPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAGGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFL 180
           IGSFFYYPSTAMHRA G FGGLR+NSRLLIPVPYADPEDDYTVLIGDWYTKSH+TLK++L
Sbjct: 121 IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQYL 180

Query: 181 DSGRSIARPDGVLINGKAAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRFQGHIMK 240
           DSGRSIARPDGVLINGK AKGDGTDEPLFTMKPGKTYKYR+CNVGLK+SLNFRFQGH MK
Sbjct: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMK 240

Query: 241 LVEMEGSHTVQNDYQSLDIHVGQCLAVLVTANQDPKDYYMVASTRFIKNVLVGKGIIRYT 300
           LVEMEGSHTVQNDYQSLD+HVGQC +VLVTANQ+ KDYYMVASTRFIKNVLVGKGI+RYT
Sbjct: 241 LVEMEGSHTVQNDYQSLDVHVGQCFSVLVTANQESKDYYMVASTRFIKNVLVGKGIVRYT 300

Query: 301 NGNGLASHEIPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVNS 360
           +G G AS EIPEAP+GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIK+VNS
Sbjct: 301 DGKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNS 360

Query: 361 AAKVDGKLRYAINGVSHIDPETPLKLAEYFGVSDKVFKYDTISDEGPAESASTVTIAPNV 420
           A+KVDGKLRYAINGVSH+DPETPLKLAEYFG++DKVFKYDTISDEG AE A+TVT+APNV
Sbjct: 361 ASKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGLAEGATTVTVAPNV 420

Query: 421 VNATFRNFVEIIFENHEKSLQSWHFDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQ 480
           VNATFR+F+EIIFENHEKSLQSWH DGYSFFAVAIEPGRW+PEKR NYNLLDAVSRHTIQ
Sbjct: 421 VNATFRDFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAILLTLDNVGMWNLRSELTENRYLGQQLYISVLSPARSLRDEYNIPDNTLLCG 540
           VFPKSWAAILLT DN GMWNLRSELTENRYLG QLYISVLSPARSLRDEYNIPDNTLLCG
Sbjct: 481 VFPKSWAAILLTFDNAGMWNLRSELTENRYLGSQLYISVLSPARSLRDEYNIPDNTLLCG 540

Query: 541 LVKDMPLPKPYTI 553
           LVKDMP+PKPYTI
Sbjct: 541 LVKDMPMPKPYTI 553

BLAST of Spg021438 vs. NCBI nr
Match: TYK21747.1 (L-ascorbate oxidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1076.6 bits (2783), Expect = 0.0e+00
Identity = 505/553 (91.32%), Postives = 531/553 (96.02%), Query Frame = 0

Query: 1   MAGLMFTTLLCLSAAAML-TVQGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MA LMFT LLCLSAAAM+ TV+GEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MARLMFTALLCLSAAAMMATVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTTNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYRFQVKDQ 120
           NSTTNNN+VINVFNNLDEPFLLHWSG+QHRKN WQDGLLGTNCPI PGTNFTY FQVKDQ
Sbjct: 61  NSTTNNNLVINVFNNLDEPFLLHWSGVQHRKNCWQDGLLGTNCPIQPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAGGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFL 180
           IGSFFYYPSTAMHRA G FGGLR+NSRLLIPVPYADPEDDYTVLIGDWYTKSH+TLK++L
Sbjct: 121 IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQYL 180

Query: 181 DSGRSIARPDGVLINGKAAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRFQGHIMK 240
           DSGRSIARPDGVLINGK AKGDGTDEPLFTMKPGKTYKYR+CNVGLK+SLNFRFQGH MK
Sbjct: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMK 240

Query: 241 LVEMEGSHTVQNDYQSLDIHVGQCLAVLVTANQDPKDYYMVASTRFIKNVLVGKGIIRYT 300
           LVEMEGSHTVQNDYQSLD+HVGQC +VLVTANQ+ KDYYMVASTRFIKNVLVGKGI+RYT
Sbjct: 241 LVEMEGSHTVQNDYQSLDVHVGQCFSVLVTANQESKDYYMVASTRFIKNVLVGKGIVRYT 300

Query: 301 NGNGLASHEIPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVNS 360
           +G G AS EIPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIK+VNS
Sbjct: 301 DGKGPASPEIPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNS 360

Query: 361 AAKVDGKLRYAINGVSHIDPETPLKLAEYFGVSDKVFKYDTISDEGPAESASTVTIAPNV 420
           A+KVDGKLRYAINGVSH+DPETPLKLAEYFG++DKVFKYDTISDEG AE A+TVT+APNV
Sbjct: 361 ASKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGLAEGATTVTVAPNV 420

Query: 421 VNATFRNFVEIIFENHEKSLQSWHFDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQ 480
           VNATFR+F+EIIFENHEKSLQSWH DGYSFFAVAIEPGRW+PEKR NYNLLDAVSRHTIQ
Sbjct: 421 VNATFRDFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAILLTLDNVGMWNLRSELTENRYLGQQLYISVLSPARSLRDEYNIPDNTLLCG 540
           VFPKSWAAILLT DN GMWNLRSELTENRYLG QLYISVLSPARSLRDEYNIPDNTLLCG
Sbjct: 481 VFPKSWAAILLTFDNAGMWNLRSELTENRYLGSQLYISVLSPARSLRDEYNIPDNTLLCG 540

Query: 541 LVKDMPLPKPYTI 553
           LVKDMP+PKPYTI
Sbjct: 541 LVKDMPMPKPYTI 553

BLAST of Spg021438 vs. ExPASy Swiss-Prot
Match: Q00624 (L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1)

HSP 1 Score: 902.5 bits (2331), Expect = 2.3e-261
Identity = 409/547 (74.77%), Postives = 466/547 (85.19%), Query Frame = 0

Query: 5   MFTTLLCLSAAAMLTVQGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTTN 64
           +    L L+AAA + V  EDPYF   WNVTYGT SPLGVPQQ ILINGQFPGPNINST+N
Sbjct: 6   LLAACLYLAAAATVVVHAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNINSTSN 65

Query: 65  NNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYRFQVKDQIGSFF 124
           NN++INVFNNLDEPFLL W+GIQHRKN WQDG  GT CPI PGTN+TY FQ KDQIGS+F
Sbjct: 66  NNVIINVFNNLDEPFLLTWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQIGSYF 125

Query: 125 YYPSTAMHRAGGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFLDSGRS 184
           YYP+T MHRA G +GGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSH+ LKKFLD GR+
Sbjct: 126 YYPTTGMHRAAGGYGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFLDGGRT 185

Query: 185 IARPDGVLINGKAAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRFQGHIMKLVEME 244
           I RPDG++INGK+ KGDG+D PLFT+KPGKTY+ RICNVG+KTS+NFR Q H MKLVEME
Sbjct: 186 IGRPDGIVINGKSGKGDGSDAPLFTLKPGKTYRVRICNVGVKTSINFRIQNHKMKLVEME 245

Query: 245 GSHTVQNDYQSLDIHVGQCLAVLVTANQDPKDYYMVASTRFIKNVLVGKGIIRYTNGNGL 304
           GSH +QNDY SLD+HVGQC   +VTANQ+PKDYYMVAS+RF+K V+   G++RY  G G 
Sbjct: 246 GSHVLQNDYDSLDVHVGQCFGTIVTANQEPKDYYMVASSRFLKTVITTTGLLRYEGGKGP 305

Query: 305 ASHEIPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVNSAAKVD 364
           AS ++P  P+GWAWSLNQFR+FRWNLTASAARPNPQGSYHYG INITRTIK+VN+  KVD
Sbjct: 306 ASSQLPAGPVGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQGKVD 365

Query: 365 GKLRYAINGVSHIDPETPLKLAEYFGVSDKVFKYDTISDEGPAESASTVTIAPNVVNATF 424
           GKLR+A+NGVSH +PETPLKLAEYFG+SDKVFKYDTI+D+   E    + I PNV+N T 
Sbjct: 366 GKLRFALNGVSHTEPETPLKLAEYFGISDKVFKYDTITDDPTPEQIKNIKIEPNVLNITH 425

Query: 425 RNFVEIIFENHEKSLQSWHFDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQVFPKS 484
           R FVE++FENHEKS+QSWH DGYSFF+VA+EPG WTPEKRKNYNLLDAVSRHT+QV+PK 
Sbjct: 426 RTFVEVVFENHEKSVQSWHLDGYSFFSVAVEPGTWTPEKRKNYNLLDAVSRHTVQVYPKC 485

Query: 485 WAAILLTLDNVGMWNLRSELTENRYLGQQLYISVLSPARSLRDEYNIPDNTLLCGLVKDM 544
           WAAILLT DN GMWN+RSE TE RYLGQQLY SVLSP +SLRDEYN+P+ +L CGLVK+ 
Sbjct: 486 WAAILLTFDNCGMWNVRSENTERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCGLVKNT 545

Query: 545 PLP-KPY 551
           P P  PY
Sbjct: 546 PKPVNPY 552

BLAST of Spg021438 vs. ExPASy Swiss-Prot
Match: P29162 (L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 828.9 bits (2140), Expect = 3.3e-239
Identity = 381/546 (69.78%), Postives = 449/546 (82.23%), Query Frame = 0

Query: 9   LLCLSAAAMLTVQGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTTNNNIV 68
           LLCLS      V  EDPY +F WNVTYGTI+PLGVPQQGILINGQFPGP IN T+NNNIV
Sbjct: 12  LLCLSVG----VIAEDPYLYFNWNVTYGTIAPLGVPQQGILINGQFPGPRINCTSNNNIV 71

Query: 69  INVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYRFQVKDQIGSFFYYPS 128
           +NVFNNLDEPFL  W+G+QHRKNSWQDG  GT CPI PG NFTYRFQVKDQIGS+ Y+P+
Sbjct: 72  VNVFNNLDEPFLFTWNGVQHRKNSWQDGTPGTMCPIMPGQNFTYRFQVKDQIGSYSYFPT 131

Query: 129 TAMHRAGGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFLDSGRSIARP 188
           TA+HRA G +G L V+SR LIPVP+ +P D+Y V +GDWY K H TLKK LD GR+I RP
Sbjct: 132 TALHRAAGGYGALNVHSRALIPVPFDNPADEYNVFVGDWYNKGHKTLKKILDGGRTIGRP 191

Query: 189 DGVLINGKAAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRFQGHIMKLVEMEGSHT 248
           DG++INGK+AK     EPLFTM+ GKTY+YR CN+G+++S+N RFQGH MKLVE+EGSHT
Sbjct: 192 DGIIINGKSAKVGEAKEPLFTMEAGKTYRYRFCNLGMRSSVNIRFQGHPMKLVELEGSHT 251

Query: 249 VQNDYQSLDIHVGQCLAVLVTANQDPKDYYMVASTRFIKNVLVGKGIIRYTNGNGLASHE 308
           VQN Y SLD+HVGQCL+VLVTA+Q+PKDYY+V S+RF+K  L    IIRY NG G AS E
Sbjct: 252 VQNIYDSLDLHVGQCLSVLVTADQEPKDYYLVVSSRFLKQALSSVAIIRYANGKGPASPE 311

Query: 309 IPEAP----MGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVNSAAKVD 368
           +P  P     G AWS+NQFR+FRWNLTASAARPNPQGSYHYG INITRTIKI NS ++V 
Sbjct: 312 LPTPPPENTEGIAWSMNQFRSFRWNLTASAARPNPQGSYHYGQINITRTIKIFNSMSQVG 371

Query: 369 GKLRYAINGVSHIDPETPLKLAEYFGVSDKVFKYDTISDEGPAESASTVTIAPNVVNATF 428
           GKLRY +NG+SH + ETPLKL EYFG ++K FKYD ++DE PA+  S +TIA NV NAT+
Sbjct: 372 GKLRYGLNGISHTNGETPLKLVEYFGATNKAFKYDLMADEAPAD-PSKLTIATNVKNATY 431

Query: 429 RNFVEIIFENHEKSLQSWHFDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQVFPKS 488
           RNFVEIIFENHEK+++++H DGYSFFAVA+EPGRW+PEKRKNYNL+D +SR+ IQV+P S
Sbjct: 432 RNFVEIIFENHEKTIRTYHLDGYSFFAVAVEPGRWSPEKRKNYNLVDGLSRNNIQVYPNS 491

Query: 489 WAAILLTLDNVGMWNLRSELTENRYLGQQLYISVLSPARSLRDEYNIPDNTLLCGLVKDM 548
           WAAI+LT DN GMWNLRSE+ E  YLG+QLY SVLSP+RSLRDEYNIPDN  LCG+VK +
Sbjct: 492 WAAIMLTFDNAGMWNLRSEMWEKTYLGEQLYFSVLSPSRSLRDEYNIPDNHPLCGIVKGL 551

Query: 549 PLPKPY 551
            +P PY
Sbjct: 552 SMPAPY 552

BLAST of Spg021438 vs. ExPASy Swiss-Prot
Match: Q9SU40 (Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=1 SV=1)

HSP 1 Score: 560.8 bits (1444), Expect = 1.7e-158
Identity = 270/543 (49.72%), Postives = 361/543 (66.48%), Query Frame = 0

Query: 24  DPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTTNNNIVINVFNNLDEPFLLHW 83
           DPY F+ + V+Y T SPLGVPQQ I ING+FPGP IN TTN N+V+NV N LDE  LLHW
Sbjct: 22  DPYSFYNFEVSYITASPLGVPQQVIAINGKFPGPTINVTTNENLVVNVRNKLDEGLLLHW 81

Query: 84  SGIQHRKNSWQDGLLGTNCPIPPGTNFTYRFQVKDQIGSFFYYPSTAMHRAGGAFGGLRV 143
           +GIQ R+ SWQDG+LGTNCPIPP  N+TY FQVKDQIGSFFY+PS    RA G FG   V
Sbjct: 82  NGIQQRRVSWQDGVLGTNCPIPPKWNWTYEFQVKDQIGSFFYFPSLHFQRASGGFGSFVV 141

Query: 144 NSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFLDSGRSIARPDGVLINGK------- 203
           N R +IPVP++ P+ D TV IGDWY ++H+ L+K LD G+ +  PDGVLINGK       
Sbjct: 142 NPRAIIPVPFSTPDGDITVTIGDWYIRNHTALRKALDDGKDLGMPDGVLINGKGPYRYND 201

Query: 204 AAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRFQGHIMKLVEMEGSHTVQNDYQSL 263
               DG D    T+ PGKTY+ R+ NVG+ TSLNFR QGH + L E EGS+TVQ +Y SL
Sbjct: 202 TLVADGIDFETITVHPGKTYRLRVSNVGISTSLNFRIQGHNLVLAESEGSYTVQQNYTSL 261

Query: 264 DIHVGQCLAVLVTANQD-PKDYYMVASTRFIKNVL----VGKGIIRYTNGNGLASHEIPE 323
           DIHVGQ  + LVT +Q+   DYY+VAS R +   +     G GI++YTN  G A  ++P 
Sbjct: 262 DIHVGQSYSFLVTMDQNASSDYYIVASARVVNETIWRRVTGVGILKYTNSKGKAKGQLPP 321

Query: 324 AP---MGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVN-SAAKVDGKL 383
            P       +S+NQ R+ RWN++AS ARPNPQGS+ YGSIN+T    + N     + GK 
Sbjct: 322 GPQDEFDKTFSMNQARSIRWNVSASGARPNPQGSFKYGSINVTDVYVLRNMPPVTISGKR 381

Query: 384 RYAINGVSHIDPETPLKLAEYFGVSDKVFKYDTISD--EGPAESASTVTIAPNVVNATFR 443
           R  +NG+S  +P TP++LA+   V D V+K D       GPA+      +A +++N T+R
Sbjct: 382 RTTLNGISFKNPSTPIRLADKLKVKD-VYKLDFPKRPLTGPAK------VATSIINGTYR 441

Query: 444 NFVEIIFENHEKSLQSWHFDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQVFPKSW 503
            F+E++ +N++  +QS+H  GY+FF V ++ G WT   R  YN  D ++R TIQV+P +W
Sbjct: 442 GFMEVVLQNNDTKMQSYHMSGYAFFVVGMDYGEWTENSRGTYNKWDGIARSTIQVYPGAW 501

Query: 504 AAILLTLDNVGMWNLRSELTENRYLGQQLYISVLSPARSLRDEYNIPDNTLLCGLVKDMP 549
           +AIL++LDN G WNLR+E  ++ YLGQ+ Y+ V++P  + + E+  PDN L CG +  + 
Sbjct: 502 SAILISLDNPGAWNLRTENLDSWYLGQETYVRVVNPDENNKTEFGHPDNVLYCGALSKLQ 557

BLAST of Spg021438 vs. ExPASy Swiss-Prot
Match: Q8VXX5 (Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=2 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 3.2e-149
Identity = 259/557 (46.50%), Postives = 372/557 (66.79%), Query Frame = 0

Query: 4   LMFTTLLCLSAAAMLTVQGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTT 63
           L+ + LLC +  + ++    DP+  + + V+Y T SPLGVPQQ I +NGQFPGP +N+TT
Sbjct: 7   LLASFLLCFALLSAVSF-AADPFVSYDFRVSYLTASPLGVPQQVIAVNGQFPGPLLNATT 66

Query: 64  NNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYRFQVKDQIGSF 123
           N N+V+NVFN+LDEP LL W GIQ R+NSWQDG+LGTNCPIPP  NFTY+FQVKDQIGSF
Sbjct: 67  NYNVVVNVFNHLDEPLLLTWPGIQMRRNSWQDGVLGTNCPIPPRWNFTYQFQVKDQIGSF 126

Query: 124 FYYPSTAMHRAGGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFLDSGR 183
           FY PS    RA G FG + +N+R +IP+P+  P+ +   +IGDWYT+ H  L++ LDSG+
Sbjct: 127 FYSPSLNFQRASGGFGPIVINNRDIIPIPFPQPDGELIFIIGDWYTQDHKALRRALDSGK 186

Query: 184 SIARPDGVLINGK------AAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRFQGHI 243
            +  PDGVLINGK      ++  DG D   F ++PGKTY+ R+ NVG+ TSLNFR Q H 
Sbjct: 187 ELGMPDGVLINGKGPYKYNSSVPDGIDYLTFHVEPGKTYRIRVHNVGISTSLNFRIQNHS 246

Query: 244 MKLVEMEGSHTVQNDYQSLDIHVGQCLAVLVTANQD-PKDYYMVASTRFIKNV----LVG 303
           + LVE EG +T Q ++   D+HVGQ  + LVT +QD   DYY+VAS RF+       + G
Sbjct: 247 LLLVETEGHYTSQANFTDFDVHVGQSYSFLVTMDQDATSDYYIVASARFVNETVWQRVTG 306

Query: 304 KGIIRYTNGNGLASH--EIPEAPMGWAWS-LNQFRTFRWNLTASAARPNPQGSYHYGSIN 363
             I+ Y+N  G  S    +P+  +   WS ++Q +T R N +AS ARPNPQGS+HYG IN
Sbjct: 307 VAILHYSNSKGPVSGPLPVPKTDVSSPWSAMSQPKTIRQNTSASGARPNPQGSFHYGQIN 366

Query: 364 ITRTIKIVNSAAK--VDGKLRYAINGVSHIDPETPLKLAEYFGVSDKVFKYDTISDEGPA 423
           IT T  I+ S     ++G LR  +NG+S ++P TP++LA+   V    +K D    + P 
Sbjct: 367 ITNTY-ILRSLPPTIINGALRATLNGISFVNPSTPVRLADRNKVKG-AYKLD--FPDRPF 426

Query: 424 ESASTVTIAPNVVNATFRNFVEIIFENHEKSLQSWHFDGYSFFAVAIEPGRWTPEKRKNY 483
                + +  +++NAT++ F++++F+N++  +QS+H DGYSFF V ++ G W+ +K+ +Y
Sbjct: 427 N--RPLRLDRSMINATYKGFIQVVFQNNDTKIQSFHVDGYSFFVVGMDFGIWSEDKKGSY 486

Query: 484 NLLDAVSRHTIQVFPKSWAAILLTLDNVGMWNLRSELTENRYLGQQLYISVLSPARSLRD 543
           N  DA+SR TI+V+P  W A+L++LDNVG+WN+R E  +  YLG++ Y+ + +P    + 
Sbjct: 487 NNWDAISRSTIEVYPGGWTAVLISLDNVGVWNIRVENLDRWYLGEETYMRITNPEEDGKT 546

Query: 544 EYNIPDNTLLCGLVKDM 545
           E + PDN L CG +K++
Sbjct: 547 EMDPPDNVLYCGALKNL 556

BLAST of Spg021438 vs. ExPASy Swiss-Prot
Match: Q9FHN6 (Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=2 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 3.2e-149
Identity = 248/538 (46.10%), Postives = 365/538 (67.84%), Query Frame = 0

Query: 24  DPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTTNNNIVINVFNNLDEPFLLHW 83
           DPY  + + ++Y T SPLGVPQQ I +NG+FPGP IN+TTN N+ +NV N+LDEP LL W
Sbjct: 25  DPYVSYDFTLSYITASPLGVPQQVIAVNGKFPGPVINATTNYNVHVNVLNHLDEPLLLTW 84

Query: 84  SGIQHRKNSWQDGLLGTNCPIPPGTNFTYRFQVKDQIGSFFYYPSTAMHRAGGAFGGLRV 143
            G+Q R+NSWQDG+LGTNCPIPP  NFTY FQ+KDQIGS+FY PS    RA G FG L +
Sbjct: 85  PGVQMRRNSWQDGVLGTNCPIPPNWNFTYDFQLKDQIGSYFYSPSLNFQRASGGFGALII 144

Query: 144 NSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFLDSGRSIARPDGVLINGK------A 203
           N+R L+P+P+ +P+ +   +IGDWYT++H+ L++ LDSG+ +  PDGVLINGK      +
Sbjct: 145 NNRDLVPIPFTEPDGEIIFIIGDWYTQNHTALRRILDSGKELGMPDGVLINGKGPFKYNS 204

Query: 204 AKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRFQGHIMKLVEMEGSHTVQNDYQSLD 263
           +  DG +     + PGKTY+ R+ NVG+ TSLNFR Q H + L+E EG +T Q ++   D
Sbjct: 205 SVPDGIEHETVNVDPGKTYRIRVHNVGISTSLNFRIQNHKLLLIETEGRYTSQMNFTDFD 264

Query: 264 IHVGQCLAVLVTANQD-PKDYYMVASTRFIKNV----LVGKGIIRYTNGNGLASHEIP-- 323
           +HVGQ  + LVT +Q+   DYY+VAS RF+       + G GI+ Y+N  G AS  +P  
Sbjct: 265 VHVGQSYSFLVTMDQNATSDYYIVASARFVNETVWQRVTGVGILHYSNSKGPASGPLPVS 324

Query: 324 EAPMGWAWS-LNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVN-SAAKVDGKLR 383
              +   WS +NQ R  + N +AS ARPNPQGS+HYG INITRT  + +    K++GKLR
Sbjct: 325 ATDVNHPWSAMNQPRAIKQNTSASGARPNPQGSFHYGQINITRTYILRSLPPTKINGKLR 384

Query: 384 YAINGVSHIDPETPLKLAEYFGVSDKVFKYDTISD--EGPAESASTVTIAPNVVNATFRN 443
             +NG+S ++P TP++LA+     D   K D + D  + P +      ++ +++NAT++ 
Sbjct: 385 ATLNGISFVNPSTPMRLAD-----DHKVKGDYMLDFPDRPLDE-KLPRLSSSIINATYKG 444

Query: 444 FVEIIFENHEKSLQSWHFDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQVFPKSWA 503
           F+++IF+N++  +QS+H DGY+F+ VA++ G W+ ++  +YN  DAV+R T++V+P +W 
Sbjct: 445 FIQVIFQNNDTKIQSFHIDGYAFYVVAMDFGIWSEDRNSSYNNWDAVARSTVEVYPGAWT 504

Query: 504 AILLTLDNVGMWNLRSELTENRYLGQQLYISVLSPARSLRDEYNIPDNTLLCGLVKDM 545
           A+L++LDNVG+WN+R E  +  YLGQ+ Y+ +++P  +   E + P+N + CG ++ M
Sbjct: 505 AVLISLDNVGVWNIRVENLDRWYLGQETYMRIINPEENGSTEMDPPENVMYCGALQAM 556

BLAST of Spg021438 vs. ExPASy TrEMBL
Match: A0A6J1C5L8 (L-ascorbate oxidase homolog OS=Momordica charantia OX=3673 GN=LOC111008621 PE=3 SV=1)

HSP 1 Score: 1083.6 bits (2801), Expect = 0.0e+00
Identity = 511/551 (92.74%), Postives = 529/551 (96.01%), Query Frame = 0

Query: 3   GLMFTTLLCLSAAAML-TVQGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINS 62
           GLMFT LLCL AAAM+ TV+GEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINS
Sbjct: 5   GLMFTMLLCLLAAAMMSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINS 64

Query: 63  TTNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYRFQVKDQIG 122
           TTNNN+V+NVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTY FQVKDQIG
Sbjct: 65  TTNNNLVVNVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQIG 124

Query: 123 SFFYYPSTAMHRAGGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFLDS 182
           S+FYYPSTAMHRA GAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWY KSHSTLK FLDS
Sbjct: 125 SYFYYPSTAMHRAAGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYXKSHSTLKNFLDS 184

Query: 183 GRSIARPDGVLINGKAAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRFQGHIMKLV 242
           GRS+ARPDGVLINGK AKGDGTDEPLFTMKPGKTYKYRICNVGLKT+LNFRFQGH MKLV
Sbjct: 185 GRSLARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRICNVGLKTTLNFRFQGHTMKLV 244

Query: 243 EMEGSHTVQNDYQSLDIHVGQCLAVLVTANQDPKDYYMVASTRFIKNVLVGKGIIRYTNG 302
           EMEGSH VQNDYQSLDIHVGQC +VLV ANQ+PKDYYMVASTRFIK++LVGKGI+RYTNG
Sbjct: 245 EMEGSHVVQNDYQSLDIHVGQCFSVLVRANQEPKDYYMVASTRFIKSLLVGKGIVRYTNG 304

Query: 303 NGLASHEIPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVNSAA 362
            G AS EIPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIK+VNSA 
Sbjct: 305 KGPASPEIPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNSAT 364

Query: 363 KVDGKLRYAINGVSHIDPETPLKLAEYFGVSDKVFKYDTISDEGPAESASTVTIAPNVVN 422
           K DGKL+YAINGVSHIDPETPLKLAEYFG++DKVFKYDTISDEGP ESA+TVTIAPNVVN
Sbjct: 365 KTDGKLQYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDEGPTESATTVTIAPNVVN 424

Query: 423 ATFRNFVEIIFENHEKSLQSWHFDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQVF 482
           ATFRNFVEIIFENHEKSLQSWHFDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQVF
Sbjct: 425 ATFRNFVEIIFENHEKSLQSWHFDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQVF 484

Query: 483 PKSWAAILLTLDNVGMWNLRSELTENRYLGQQLYISVLSPARSLRDEYNIPDNTLLCGLV 542
           PKSWAAILLTLDNVGMWNLRSELTENRYLGQQLYISV+SPA SLRDEYNIPDN LLCGLV
Sbjct: 485 PKSWAAILLTLDNVGMWNLRSELTENRYLGQQLYISVISPAHSLRDEYNIPDNALLCGLV 544

Query: 543 KDMPLPKPYTI 553
           K MPLP PYTI
Sbjct: 545 KGMPLPXPYTI 555

BLAST of Spg021438 vs. ExPASy TrEMBL
Match: A0A5A7T9Z0 (L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G001230 PE=3 SV=1)

HSP 1 Score: 1081.2 bits (2795), Expect = 0.0e+00
Identity = 508/553 (91.86%), Postives = 531/553 (96.02%), Query Frame = 0

Query: 1   MAGLMFTTLLCLSAAAMLT-VQGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MAGLMFTTLLCLSAAAM T V+GEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MAGLMFTTLLCLSAAAMTTMVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTTNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYRFQVKDQ 120
           NSTTNNN+VINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTY FQVKDQ
Sbjct: 61  NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAGGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFL 180
           IGSFFYYPSTAMHRA G FGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSH+TLK+FL
Sbjct: 121 IGSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQFL 180

Query: 181 DSGRSIARPDGVLINGKAAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRFQGHIMK 240
           DSGRSIARPDGVLINGK AKGDGTDEPLFTMKPGKTYKYR+CNVGLKTSLNFRFQGH MK
Sbjct: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTMK 240

Query: 241 LVEMEGSHTVQNDYQSLDIHVGQCLAVLVTANQDPKDYYMVASTRFIKNVLVGKGIIRYT 300
           LVEMEGSHTVQNDY+SLD+HVGQC +VLVTA+Q+PKDYYMVASTRFIK+ LVGKGI+RYT
Sbjct: 241 LVEMEGSHTVQNDYKSLDVHVGQCFSVLVTADQEPKDYYMVASTRFIKSALVGKGIVRYT 300

Query: 301 NGNGLASHEIPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVNS 360
           NG G AS E+P APMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIK+VNS
Sbjct: 301 NGKGPASPELPNAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNS 360

Query: 361 AAKVDGKLRYAINGVSHIDPETPLKLAEYFGVSDKVFKYDTISDEGPAESASTVTIAPNV 420
           A+KVDGKLRYAINGVSH+DPETPLKLAEYFGV+DKVFKYDTISDEG AE A+TVT+APNV
Sbjct: 361 ASKVDGKLRYAINGVSHVDPETPLKLAEYFGVTDKVFKYDTISDEGLAEGATTVTVAPNV 420

Query: 421 VNATFRNFVEIIFENHEKSLQSWHFDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQ 480
           VN TFRNF+EIIFENHEKSLQSWH DGYSFFAVAIEPGRWTPEKR +YNLLDAVSRHTIQ
Sbjct: 421 VNTTFRNFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWTPEKRSDYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAILLTLDNVGMWNLRSELTENRYLGQQLYISVLSPARSLRDEYNIPDNTLLCG 540
           VFPKSWAAILLT DN GMWNLRSEL ENRYLGQQLY+SV SPARSLRDEYNIPDNTLLCG
Sbjct: 481 VFPKSWAAILLTFDNAGMWNLRSELAENRYLGQQLYMSVQSPARSLRDEYNIPDNTLLCG 540

Query: 541 LVKDMPLPKPYTI 553
           LVKD+PLPKPYTI
Sbjct: 541 LVKDLPLPKPYTI 553

BLAST of Spg021438 vs. ExPASy TrEMBL
Match: A0A6J1DK02 (L-ascorbate oxidase homolog OS=Momordica charantia OX=3673 GN=LOC111021193 PE=3 SV=1)

HSP 1 Score: 1081.2 bits (2795), Expect = 0.0e+00
Identity = 505/552 (91.49%), Postives = 528/552 (95.65%), Query Frame = 0

Query: 1   MAGLMFTTLLCLSAAAMLTVQGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNIN 60
           MAGLMFT LLCLSAAAM TV+GEDPYFFFTWN+TYGTISPLGVPQQGILINGQFPGPNIN
Sbjct: 1   MAGLMFTVLLCLSAAAMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNIN 60

Query: 61  STTNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYRFQVKDQI 120
           ST+NNNIV+NVFNNLDEPFL+HWSGIQHRKNSWQDGLLGTNCPIPPGTN+TY FQVKDQI
Sbjct: 61  STSNNNIVVNVFNNLDEPFLIHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI 120

Query: 121 GSFFYYPSTAMHRAGGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFLD 180
           GSFFYYPSTAMHRA G FGGLRVNSRLLIPVPYADPEDDYTVL+GDWYTKSHS LK FLD
Sbjct: 121 GSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLVGDWYTKSHSALKNFLD 180

Query: 181 SGRSIARPDGVLINGKAAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRFQGHIMKL 240
           SGRS+ARPDGVLINGK AKGDGTDEPLFTMKP KTYKYR+CN+GLKTSLN RFQ H MKL
Sbjct: 181 SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQDHTMKL 240

Query: 241 VEMEGSHTVQNDYQSLDIHVGQCLAVLVTANQDPKDYYMVASTRFIKNVLVGKGIIRYTN 300
           VEMEGSH VQNDYQSLDIHVGQC +VLVTANQ+PKDYY++ASTRF KNVLVGKGIIRYTN
Sbjct: 241 VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN 300

Query: 301 GNGLASHEIPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVNSA 360
           G G AS EIPEAP+GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKI+NSA
Sbjct: 301 GKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA 360

Query: 361 AKVDGKLRYAINGVSHIDPETPLKLAEYFGVSDKVFKYDTISDEGPAESASTVTIAPNVV 420
            KVDGKLRYAINGVSHIDPETPLKLAEYFG++DKVFKYDTISD+GPAE A+TVT+APNVV
Sbjct: 361 TKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVV 420

Query: 421 NATFRNFVEIIFENHEKSLQSWHFDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQV 480
           NATFRNFVEI+FENHEKSLQSWH +GYSFFAVAIEPGRWTPEKRKNYNLLDAVSR TIQV
Sbjct: 421 NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRDTIQV 480

Query: 481 FPKSWAAILLTLDNVGMWNLRSELTENRYLGQQLYISVLSPARSLRDEYNIPDNTLLCGL 540
           FPKSWAAILLTLDN GMWNLRSELTENRYLGQQLY SVLSPARSLRDEYNIPDNTLLCGL
Sbjct: 481 FPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL 540

Query: 541 VKDMPLPKPYTI 553
           VKDMPLPKPYTI
Sbjct: 541 VKDMPLPKPYTI 552

BLAST of Spg021438 vs. ExPASy TrEMBL
Match: A0A5A7TAM1 (L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold366G00980 PE=3 SV=1)

HSP 1 Score: 1080.5 bits (2793), Expect = 0.0e+00
Identity = 507/553 (91.68%), Postives = 533/553 (96.38%), Query Frame = 0

Query: 1   MAGLMFTTLLCLSAAAML-TVQGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MAGLMFT LLCLSAAAM+ TVQGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MAGLMFTALLCLSAAAMMATVQGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTTNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYRFQVKDQ 120
           NST+NNN+VINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPI PGTNFTY FQVKDQ
Sbjct: 61  NSTSNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIQPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAGGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFL 180
           IGSFFYYPSTAMHRA G FGGLR+NSRLLIPVPYADPEDDYTVLIGDWYTKSH+TLK++L
Sbjct: 121 IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQYL 180

Query: 181 DSGRSIARPDGVLINGKAAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRFQGHIMK 240
           DSGRSIARPDGVLINGK AKGDGTDEPLFTMKPGKTYKYR+CNVGLK+SLNFRFQGH MK
Sbjct: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMK 240

Query: 241 LVEMEGSHTVQNDYQSLDIHVGQCLAVLVTANQDPKDYYMVASTRFIKNVLVGKGIIRYT 300
           LVEMEGSHTVQNDYQSLD+HVGQC +VLVTANQ+ KDYYMVASTRFIKNVLVGKGI+RYT
Sbjct: 241 LVEMEGSHTVQNDYQSLDVHVGQCFSVLVTANQESKDYYMVASTRFIKNVLVGKGIVRYT 300

Query: 301 NGNGLASHEIPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVNS 360
           +G G AS EIPEAP+GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIK+VNS
Sbjct: 301 DGKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNS 360

Query: 361 AAKVDGKLRYAINGVSHIDPETPLKLAEYFGVSDKVFKYDTISDEGPAESASTVTIAPNV 420
           A+KVDGKLRYAINGVSH+DPETPLKLAEYFG++DKVFKYDTISDEG AE A+TVT+APNV
Sbjct: 361 ASKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGLAEGATTVTVAPNV 420

Query: 421 VNATFRNFVEIIFENHEKSLQSWHFDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQ 480
           VNATFR+F+EIIFENHEKSLQSWH DGYSFFAVAIEPGRW+PEKR NYNLLDAVSRHTIQ
Sbjct: 421 VNATFRDFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAILLTLDNVGMWNLRSELTENRYLGQQLYISVLSPARSLRDEYNIPDNTLLCG 540
           VFPKSWAAILLT DN GMWNLRSELTENRYLG QLYISVLSPARSLRDEYNIPDNTLLCG
Sbjct: 481 VFPKSWAAILLTFDNAGMWNLRSELTENRYLGSQLYISVLSPARSLRDEYNIPDNTLLCG 540

Query: 541 LVKDMPLPKPYTI 553
           LVKDMP+PKPYTI
Sbjct: 541 LVKDMPMPKPYTI 553

BLAST of Spg021438 vs. ExPASy TrEMBL
Match: A0A5D3DDJ5 (L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G001190 PE=3 SV=1)

HSP 1 Score: 1076.6 bits (2783), Expect = 0.0e+00
Identity = 505/553 (91.32%), Postives = 531/553 (96.02%), Query Frame = 0

Query: 1   MAGLMFTTLLCLSAAAML-TVQGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MA LMFT LLCLSAAAM+ TV+GEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MARLMFTALLCLSAAAMMATVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTTNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYRFQVKDQ 120
           NSTTNNN+VINVFNNLDEPFLLHWSG+QHRKN WQDGLLGTNCPI PGTNFTY FQVKDQ
Sbjct: 61  NSTTNNNLVINVFNNLDEPFLLHWSGVQHRKNCWQDGLLGTNCPIQPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAGGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFL 180
           IGSFFYYPSTAMHRA G FGGLR+NSRLLIPVPYADPEDDYTVLIGDWYTKSH+TLK++L
Sbjct: 121 IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQYL 180

Query: 181 DSGRSIARPDGVLINGKAAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRFQGHIMK 240
           DSGRSIARPDGVLINGK AKGDGTDEPLFTMKPGKTYKYR+CNVGLK+SLNFRFQGH MK
Sbjct: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMK 240

Query: 241 LVEMEGSHTVQNDYQSLDIHVGQCLAVLVTANQDPKDYYMVASTRFIKNVLVGKGIIRYT 300
           LVEMEGSHTVQNDYQSLD+HVGQC +VLVTANQ+ KDYYMVASTRFIKNVLVGKGI+RYT
Sbjct: 241 LVEMEGSHTVQNDYQSLDVHVGQCFSVLVTANQESKDYYMVASTRFIKNVLVGKGIVRYT 300

Query: 301 NGNGLASHEIPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVNS 360
           +G G AS EIPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIK+VNS
Sbjct: 301 DGKGPASPEIPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNS 360

Query: 361 AAKVDGKLRYAINGVSHIDPETPLKLAEYFGVSDKVFKYDTISDEGPAESASTVTIAPNV 420
           A+KVDGKLRYAINGVSH+DPETPLKLAEYFG++DKVFKYDTISDEG AE A+TVT+APNV
Sbjct: 361 ASKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGLAEGATTVTVAPNV 420

Query: 421 VNATFRNFVEIIFENHEKSLQSWHFDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQ 480
           VNATFR+F+EIIFENHEKSLQSWH DGYSFFAVAIEPGRW+PEKR NYNLLDAVSRHTIQ
Sbjct: 421 VNATFRDFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAILLTLDNVGMWNLRSELTENRYLGQQLYISVLSPARSLRDEYNIPDNTLLCG 540
           VFPKSWAAILLT DN GMWNLRSELTENRYLG QLYISVLSPARSLRDEYNIPDNTLLCG
Sbjct: 481 VFPKSWAAILLTFDNAGMWNLRSELTENRYLGSQLYISVLSPARSLRDEYNIPDNTLLCG 540

Query: 541 LVKDMPLPKPYTI 553
           LVKDMP+PKPYTI
Sbjct: 541 LVKDMPMPKPYTI 553

BLAST of Spg021438 vs. TAIR 10
Match: AT3G13390.1 (SKU5 similar 11 )

HSP 1 Score: 909.1 bits (2348), Expect = 1.8e-264
Identity = 410/546 (75.09%), Postives = 469/546 (85.90%), Query Frame = 0

Query: 5   MFTTLLCLSAAAMLTVQGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTTN 64
           +    L L+AAA + V+ EDPYF   WNVTYGT+SPLGVPQQ ILINGQFPGPN+NST+N
Sbjct: 6   LLAACLYLAAAATVVVRAEDPYFHHVWNVTYGTVSPLGVPQQVILINGQFPGPNVNSTSN 65

Query: 65  NNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYRFQVKDQIGSFF 124
           NN++INVFNNLDEPFLL W+GIQHRKN WQDG  GT CPI PGTN+TY FQ KDQIGS+F
Sbjct: 66  NNVIINVFNNLDEPFLLTWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQIGSYF 125

Query: 125 YYPSTAMHRAGGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFLDSGRS 184
           YYPSTAMHR+ G FGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSH+ LKKFLDSGR+
Sbjct: 126 YYPSTAMHRSAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFLDSGRT 185

Query: 185 IARPDGVLINGKAAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRFQGHIMKLVEME 244
           + RPDG+LINGK+ KGDG+D PLFT+KPGKTY+ RICNVGLKTSLNFR Q H +KLVEME
Sbjct: 186 LGRPDGILINGKSGKGDGSDAPLFTLKPGKTYRVRICNVGLKTSLNFRIQNHKLKLVEME 245

Query: 245 GSHTVQNDYQSLDIHVGQCLAVLVTANQDPKDYYMVASTRFIKNVLVGKGIIRYTNGNGL 304
           GSH +QNDY SLD+HVGQC   ++TANQ+ KDYYMVAS+RF+K+V+   G++RY  G G 
Sbjct: 246 GSHVLQNDYDSLDVHVGQCYGTILTANQEAKDYYMVASSRFLKSVITTTGLLRYEGGKGP 305

Query: 305 ASHEIPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVNSAAKVD 364
           AS ++P  P+GWAWSLNQFR+FRWNLTASAARPNPQGSYHYG INITRTIK+VN+  KVD
Sbjct: 306 ASSQLPPGPVGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQGKVD 365

Query: 365 GKLRYAINGVSHIDPETPLKLAEYFGVSDKVFKYDTISDEGPAESASTVTIAPNVVNATF 424
           GKLRYA+NGVSH DPETPLKLAEYFGV+DKVFKYD+I+D    E   ++ I PNV+N T 
Sbjct: 366 GKLRYALNGVSHTDPETPLKLAEYFGVADKVFKYDSITDNPTPEQIKSIKIVPNVLNITH 425

Query: 425 RNFVEIIFENHEKSLQSWHFDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQVFPKS 484
           R F+E++FENHEKS+QSWH DGYSFFAVA+EPG WTPEKRKNYNLLDAVSRHT+QV+PK 
Sbjct: 426 RTFIEVVFENHEKSVQSWHLDGYSFFAVAVEPGTWTPEKRKNYNLLDAVSRHTVQVYPKC 485

Query: 485 WAAILLTLDNVGMWNLRSELTENRYLGQQLYISVLSPARSLRDEYNIPDNTLLCGLVKDM 544
           WAAILLT DN GMWN+RSE +E RYLGQQLY SVLSP +SLRDEYN+P+ +L CGLVK  
Sbjct: 486 WAAILLTFDNCGMWNVRSENSERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCGLVKGT 545

Query: 545 PLPKPY 551
           P P PY
Sbjct: 546 PKPNPY 551

BLAST of Spg021438 vs. TAIR 10
Match: AT1G55570.1 (SKU5 similar 12 )

HSP 1 Score: 904.8 bits (2337), Expect = 3.3e-263
Identity = 407/546 (74.54%), Postives = 464/546 (84.98%), Query Frame = 0

Query: 5   MFTTLLCLSAAAMLTVQGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTTN 64
           +    LC++ A ++ VQ EDPYF   WNVTYGT SPLGVPQQ ILINGQFPGPNINST+N
Sbjct: 7   LLAVCLCVATATVMMVQAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNINSTSN 66

Query: 65  NNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYRFQVKDQIGSFF 124
           NN+++NVFNNLDEPFL+ W+GIQHRKN WQDG  GT CPIPPG NFTY FQ KDQIGS+F
Sbjct: 67  NNVIVNVFNNLDEPFLITWAGIQHRKNCWQDGTAGTMCPIPPGQNFTYHFQPKDQIGSYF 126

Query: 125 YYPSTAMHRAGGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFLDSGRS 184
           YYP+TAMHRA G FGGLRVNSRLLIPVPYADPEDDYT+LI DWYTKSH+ LKKFLDSGR+
Sbjct: 127 YYPTTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTILINDWYTKSHTQLKKFLDSGRT 186

Query: 185 IARPDGVLINGKAAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRFQGHIMKLVEME 244
           I RPDG+LINGK+ K DG+D+PLFT+KPGKTY+ RICNVGLK SLNFR Q H MKLVEME
Sbjct: 187 IGRPDGILINGKSGKTDGSDKPLFTLKPGKTYRVRICNVGLKASLNFRIQNHKMKLVEME 246

Query: 245 GSHTVQNDYQSLDIHVGQCLAVLVTANQDPKDYYMVASTRFIKNVLVGKGIIRYTNGNGL 304
           GSH +QNDY SLD+HVGQC  V+VTA+Q+PKDYYM+ASTRF+K  L   G++RY  G G 
Sbjct: 247 GSHVLQNDYDSLDVHVGQCFGVIVTADQEPKDYYMIASTRFLKKPLTTTGLLRYEGGKGP 306

Query: 305 ASHEIPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVNSAAKVD 364
           AS ++P AP+GWAWSLNQ+R+FRWNLTASAARPNPQGSYHYG INITRTIK+VN+  KVD
Sbjct: 307 ASSQLPAAPVGWAWSLNQYRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQGKVD 366

Query: 365 GKLRYAINGVSHIDPETPLKLAEYFGVSDKVFKYDTISDEGPAESASTVTIAPNVVNATF 424
           GKLRYA++GVSH DPETPLKLAEYFGV+DKVFKYDTISD    +    + I PNV+N T 
Sbjct: 367 GKLRYALSGVSHTDPETPLKLAEYFGVADKVFKYDTISDNPNPDQIKNIKIEPNVLNITH 426

Query: 425 RNFVEIIFENHEKSLQSWHFDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQVFPKS 484
           R F+E++FENHE+S+QSWH DGYSFFAVA+EPG WTPEKRKNYNLLDAVSRHT+QV+PK 
Sbjct: 427 RTFIEVVFENHERSVQSWHLDGYSFFAVAVEPGTWTPEKRKNYNLLDAVSRHTVQVYPKC 486

Query: 485 WAAILLTLDNVGMWNLRSELTENRYLGQQLYISVLSPARSLRDEYNIPDNTLLCGLVKDM 544
           WAAILLT DN GMWN+RSE  E RYLGQQLY SVLSP +SLRDEYN+P+ +L CGLVK  
Sbjct: 487 WAAILLTFDNCGMWNIRSENAERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCGLVKGK 546

Query: 545 PLPKPY 551
           P   PY
Sbjct: 547 PKVNPY 552

BLAST of Spg021438 vs. TAIR 10
Match: AT3G13400.1 (SKU5 similar 13 )

HSP 1 Score: 885.6 bits (2287), Expect = 2.1e-257
Identity = 407/550 (74.00%), Postives = 468/550 (85.09%), Query Frame = 0

Query: 3   GLMFTTLLCLSAAAMLTVQGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINST 62
           G + T L+CL++   L   G DPYF++TWNVTYGT +PLG+PQQ ILINGQFPGPN+NST
Sbjct: 4   GRLLTVLVCLASTVALVSAG-DPYFYYTWNVTYGTAAPLGIPQQVILINGQFPGPNLNST 63

Query: 63  TNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYRFQVKDQIGS 122
           +NNN+VINVFNNLDEPFLL WSG+QHRKNSWQDG+ GT+CPIP GTNFTY FQ KDQIGS
Sbjct: 64  SNNNVVINVFNNLDEPFLLTWSGLQHRKNSWQDGVTGTSCPIPAGTNFTYHFQPKDQIGS 123

Query: 123 FFYYPSTAMHRAGGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFLDSG 182
           +FYYPSTA+HR  G FGGLRVNSRLLIPVPYADPEDD T+LI DWY KSH+ LK FLDSG
Sbjct: 124 YFYYPSTALHRFAGGFGGLRVNSRLLIPVPYADPEDDRTILINDWYAKSHTALKNFLDSG 183

Query: 183 RSIARPDGVLINGKAAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRFQGHIMKLVE 242
           R++  PDGVLINGK+ K  G + PLFTMKPGKTYKYRICNVG K++LNFR QGH MKLVE
Sbjct: 184 RTLGSPDGVLINGKSGKLGGNNAPLFTMKPGKTYKYRICNVGFKSTLNFRIQGHKMKLVE 243

Query: 243 MEGSHTVQNDYQSLDIHVGQCLAVLVTANQDPKDYYMVASTRFIKNVLVGKGIIRYTNGN 302
           MEGSH +QNDY SLD+HVGQC AVLVTA+Q  K+YYMVASTRF+K  +   G++ Y   N
Sbjct: 244 MEGSHVLQNDYDSLDVHVGQCFAVLVTADQVAKNYYMVASTRFLKKEVSTVGVMSYEGSN 303

Query: 303 GLASHEIPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVNSAAK 362
             AS +IP+AP+GWAWSLNQFR+FRWNLTASAARPNPQGSYHYG INITRTIK+ N+   
Sbjct: 304 VQASSDIPKAPVGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLANTKNL 363

Query: 363 VDGKLRYAINGVSHIDPETPLKLAEYFGVSDKVFKYDTISDEGPAESASTVTIAPNVVNA 422
           V+GK+R+  NGVSH+D ETPLKLAEYFG+S+KVFKY+ I DE PA   +T+T+ PNV+N 
Sbjct: 364 VNGKVRFGFNGVSHVDTETPLKLAEYFGMSEKVFKYNVIKDE-PAAKITTLTVEPNVLNI 423

Query: 423 TFRNFVEIIFENHEKSLQSWHFDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQVFP 482
           TFR FVE++FENHEKS+QS+H DGYSFFAVA EPGRWTPEKR NYNLLDAVSRHT+QV+P
Sbjct: 424 TFRTFVEVVFENHEKSMQSFHLDGYSFFAVASEPGRWTPEKRNNYNLLDAVSRHTVQVYP 483

Query: 483 KSWAAILLTLDNVGMWNLRSELTENRYLGQQLYISVLSPARSLRDEYNIPDNTLLCGLVK 542
           KSW+AILLT DN GMWN+RSE  E RYLGQQLY+SVLSP +SLRDEYNIP NT LCG+VK
Sbjct: 484 KSWSAILLTFDNAGMWNIRSENWERRYLGQQLYVSVLSPEKSLRDEYNIPLNTNLCGIVK 543

Query: 543 DMPLPKPYTI 553
            +PLP PYTI
Sbjct: 544 GLPLPTPYTI 551

BLAST of Spg021438 vs. TAIR 10
Match: AT1G55560.1 (SKU5 similar 14 )

HSP 1 Score: 873.6 bits (2256), Expect = 8.3e-254
Identity = 402/551 (72.96%), Postives = 466/551 (84.57%), Query Frame = 0

Query: 1   MAGLMFTTLLCLSAAAMLTVQGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNIN 60
           M G + T L+CL  + +  V   DPYFF TWNVTYGT SPLGVPQ+ ILINGQFPGPN+N
Sbjct: 1   MEGRLLTVLVCL-VSTVAIVNAGDPYFFHTWNVTYGTASPLGVPQKVILINGQFPGPNLN 60

Query: 61  STTNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYRFQVKDQI 120
           ST+NNN+VINVFN+LDEPFLL WSGIQHRKN WQDG+ GT+CPIP G NFTY FQ KDQI
Sbjct: 61  STSNNNVVINVFNHLDEPFLLTWSGIQHRKNCWQDGVAGTSCPIPAGQNFTYHFQPKDQI 120

Query: 121 GSFFYYPSTAMHRAGGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFLD 180
           GS+FYYP+T++HR  G FGGLRVNSRLLIPVPYADPEDDYTVL+GDWYT  H+ LK FLD
Sbjct: 121 GSYFYYPTTSLHRFAGGFGGLRVNSRLLIPVPYADPEDDYTVLLGDWYTAGHTALKNFLD 180

Query: 181 SGRSIARPDGVLINGKAAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRFQGHIMKL 240
           SGR++  P+GVLINGK+ K  G +EPLFTMKPGKTYKYR+CNVG K++LNFR Q H MKL
Sbjct: 181 SGRTLGLPNGVLINGKSGKVGGKNEPLFTMKPGKTYKYRLCNVGFKSTLNFRIQNHKMKL 240

Query: 241 VEMEGSHTVQNDYQSLDIHVGQCLAVLVTANQDPKDYYMVASTRFIKNVLVGKGIIRYTN 300
           VEMEGSH +QNDY SLD+HVGQC +VLVTANQ  KDYYMVASTRF+K  L   G+IRY  
Sbjct: 241 VEMEGSHVIQNDYDSLDVHVGQCFSVLVTANQAAKDYYMVASTRFLKKELSTVGVIRYEG 300

Query: 301 GNGLASHEIPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVNSA 360
            N  AS E+P+AP+GWAWSLNQFR+FRWNLT++AARPNPQGSYHYG INITR+IK+VNS 
Sbjct: 301 SNVQASTELPKAPVGWAWSLNQFRSFRWNLTSNAARPNPQGSYHYGKINITRSIKLVNSK 360

Query: 361 AKVDGKLRYAINGVSHIDPETPLKLAEYFGVSDKVFKYDTISDEGPAESASTVTIAPNVV 420
           + VDGK+R+  NGVSH+D ETPLKLAEYF +S+KVFKY+ I DE PA   + +T+ PNV+
Sbjct: 361 SVVDGKVRFGFNGVSHVDTETPLKLAEYFQMSEKVFKYNVIKDE-PAAKITALTVQPNVL 420

Query: 421 NATFRNFVEIIFENHEKSLQSWHFDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQV 480
           N TFR FVEIIFENHEK++QS+H DGYSFFAVA EPGRWTPEKR+NYNLLDAVSRHT+QV
Sbjct: 421 NITFRTFVEIIFENHEKTMQSFHLDGYSFFAVASEPGRWTPEKRENYNLLDAVSRHTVQV 480

Query: 481 FPKSWAAILLTLDNVGMWNLRSELTENRYLGQQLYISVLSPARSLRDEYNIPDNTLLCGL 540
           +PKSW+AILLT DN GMWN+RSE  E +YLG+QLY+SVLSP +SLRDEYNIP NT LCG+
Sbjct: 481 YPKSWSAILLTFDNAGMWNIRSENLERKYLGEQLYVSVLSPEKSLRDEYNIPLNTNLCGI 540

Query: 541 VKDMPLPKPYT 552
           VK +PLP  Y+
Sbjct: 541 VKGLPLPAHYS 549

BLAST of Spg021438 vs. TAIR 10
Match: AT1G21860.1 (SKU5 similar 7 )

HSP 1 Score: 639.4 bits (1648), Expect = 2.6e-183
Identity = 305/535 (57.01%), Postives = 386/535 (72.15%), Query Frame = 0

Query: 5   MFTTLLCLSAAAMLTVQGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTTN 64
           M TTLL L + A      EDPY FF W+VTYG ISPLGV QQGILING+FPGP+I S TN
Sbjct: 11  MITTLLFLISLAF----AEDPYRFFEWHVTYGNISPLGVAQQGILINGKFPGPDIISITN 70

Query: 65  NNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYRFQVKDQIGSFF 124
           +N++INVFN+LDEPFLL W+GI++ KNS+QDG+ GT CPIPPG N+TY  QVKDQIGSF+
Sbjct: 71  DNLIINVFNHLDEPFLLSWNGIRNWKNSFQDGVYGTMCPIPPGKNYTYALQVKDQIGSFY 130

Query: 125 YYPSTAMHRAGGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFLDSGRS 184
           Y+PS   H+A G FGG+R++SR LIPVP+  P DDYT+L+GDWY  +H  LK  LD+G  
Sbjct: 131 YFPSLGFHKAAGGFGGIRISSRALIPVPFPTPADDYTLLVGDWYKTNHKDLKAQLDNGGK 190

Query: 185 IARPDGVLINGKAAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRFQGHIMKLVEME 244
           +  PDG+LING+++           ++PGKTY+ RI NVGL+ SLNFR Q H MKLVE+E
Sbjct: 191 LPLPDGILINGRSSGA------TLNIEPGKTYRLRISNVGLQNSLNFRIQNHTMKLVEVE 250

Query: 245 GSHTVQNDYQSLDIHVGQCLAVLVTANQDPKDYYMVASTRFIKNVLVGKGIIRYTNGNGL 304
           G +T+QN + SLD+HVGQ  +VL+TA+Q  KDYY+V S+RF   +L   G++ Y+N    
Sbjct: 251 GRYTIQNLFSSLDVHVGQSYSVLITADQPAKDYYVVVSSRFTSKILTTTGVLHYSNSVAP 310

Query: 305 ASHEIPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVNSAAKVD 364
            S  IP+ P+  +WS NQ R  R NLTAS  RPNPQGSY YG INITRTI++ N+   ++
Sbjct: 311 VSGPIPDGPIKLSWSFNQARAIRTNLTASGPRPNPQGSYRYGVINITRTIRLANNLGHIE 370

Query: 365 GKLRYAINGVSHIDPETPLKLAEYFGVSDKVFKYDTISDEGPAESASTVTIAPNVVNATF 424
           GK RYA+N  S    +TPLKL +YF + D V+K  +ISD+ P   A   T   +V+ A F
Sbjct: 371 GKQRYAVNSASFYPADTPLKLVDYFKI-DGVYKPGSISDQ-PTNGAIFPT--TSVMQADF 430

Query: 425 RNFVEIIFENHEKSLQSWHFDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQVFPKS 484
           R FVE+IFEN E  +QSWH DGYSF+ V +E G+W+P  RK YNL DA+ R TIQV+P+S
Sbjct: 431 RAFVEVIFENSEDIVQSWHLDGYSFYVVGMELGKWSPASRKVYNLNDAILRCTIQVYPRS 490

Query: 485 WAAILLTLDNVGMWNLRSELTENRYLGQQLYISVLSPARSLRDEYNIPDNTLLCG 540
           W AI + LDNVGMWN+RSE+ E +YLGQQ Y+ V + + SLRDEY IP N LLCG
Sbjct: 491 WTAIYIALDNVGMWNMRSEIWERQYLGQQFYMRVYTTSTSLRDEYLIPKNALLCG 531

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022137060.10.0e+0092.74L-ascorbate oxidase homolog [Momordica charantia][more]
XP_022153749.10.0e+0091.49L-ascorbate oxidase homolog [Momordica charantia][more]
KAA0040130.10.0e+0091.86L-ascorbate oxidase-like protein [Cucumis melo var. makuwa] >TYK21751.1 L-ascorb... [more]
KAA0040123.10.0e+0091.68L-ascorbate oxidase-like protein [Cucumis melo var. makuwa][more]
TYK21747.10.0e+0091.32L-ascorbate oxidase-like protein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q006242.3e-26174.77L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1[more]
P291623.3e-23969.78L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q9SU401.7e-15849.72Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=... [more]
Q8VXX53.2e-14946.50Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=... [more]
Q9FHN63.2e-14946.10Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1C5L80.0e+0092.74L-ascorbate oxidase homolog OS=Momordica charantia OX=3673 GN=LOC111008621 PE=3 ... [more]
A0A5A7T9Z00.0e+0091.86L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A6J1DK020.0e+0091.49L-ascorbate oxidase homolog OS=Momordica charantia OX=3673 GN=LOC111021193 PE=3 ... [more]
A0A5A7TAM10.0e+0091.68L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A5D3DDJ50.0e+0091.32L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
Match NameE-valueIdentityDescription
AT3G13390.11.8e-26475.09SKU5 similar 11 [more]
AT1G55570.13.3e-26374.54SKU5 similar 12 [more]
AT3G13400.12.1e-25774.00SKU5 similar 13 [more]
AT1G55560.18.3e-25472.96SKU5 similar 14 [more]
AT1G21860.12.6e-18357.01SKU5 similar 7 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011707Multicopper oxidase, N-termianlPFAMPF07732Cu-oxidase_3coord: 32..146
e-value: 5.6E-40
score: 136.1
IPR011706Multicopper oxidase, C-terminalPFAMPF07731Cu-oxidase_2coord: 382..520
e-value: 1.3E-23
score: 83.2
IPR008972CupredoxinGENE3D2.60.40.420coord: 346..545
e-value: 5.2E-34
score: 119.7
IPR008972CupredoxinGENE3D2.60.40.420coord: 28..147
e-value: 3.2E-34
score: 119.8
IPR008972CupredoxinGENE3D2.60.40.420coord: 156..336
e-value: 1.8E-34
score: 121.6
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 23..172
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 155..337
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 348..538
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 158..298
e-value: 5.4E-40
score: 137.1
NoneNo IPR availablePANTHERPTHR11709:SF246L-ASCORBATE OXIDASE-LIKE PROTEINcoord: 4..543
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 4..543
IPR034271Ascorbate oxidase homologue, second cupredoxin domainCDDcd13872CuRO_2_AAO_like_1coord: 158..298
e-value: 9.87416E-87
score: 262.724

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg021438.1Spg021438.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005507 copper ion binding
molecular_function GO:0016491 oxidoreductase activity