Spg021421 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg021421 vs. NCBI nr
Match: XP_038887700.1 (nuclear-pore anchor isoform X2 [Benincasa hispida])

HSP 1 Score: 1009.6 bits (2609), Expect = 1.8e-290
Identity = 581/707 (82.18%), Postives = 611/707 (86.42%), Query Frame = 0

Query: 217 IIVRQEDLCEEAMEEENTAEHERMIESYSLLNQKLQNSISEQAILEKTVQELKADLKRHE 276
           ++   ED  E  +EE   AEHERMIESYSLLNQKLQNSISEQAILEKT+QELKADLKRHE
Sbjct: 190 VLYELEDKAEVILEER--AEHERMIESYSLLNQKLQNSISEQAILEKTLQELKADLKRHE 249

Query: 277 RDYTLIQRENIDLSRQVTILLKECRDVQLRCGYTGHDVLENVSNTTSFEINVGSDADKVI 336
           RDY LIQRENIDLSRQ+TILLKECRDVQLRCGY GHDVLEN+S TTS EINV SDAD+VI
Sbjct: 250 RDYLLIQRENIDLSRQITILLKECRDVQLRCGYAGHDVLENLSKTTSLEINVESDADRVI 309

Query: 337 SEYLLTFKDINGLVEQNVQLRSLVRKLSVQLQDTELDFKEKLEVELKRQTQEAASRVEAV 396
           SEYLLTFKDINGLVEQNVQLRSLVRKLSVQLQD ELDFKE LEVELKR+TQEAASRVEAV
Sbjct: 310 SEYLLTFKDINGLVEQNVQLRSLVRKLSVQLQDAELDFKENLEVELKRKTQEAASRVEAV 369

Query: 397 LQKVEEQGQMIESLHASVAMYKRLYEEEHKRNLPHPLSAGVALGFGRKEQEFVSEDSQEA 456
           L+KVEEQGQMIESLHASV+MYKRLYEEEHKRNLP  LSAG AL FGRKE EFV+EDSQEA
Sbjct: 370 LKKVEEQGQMIESLHASVSMYKRLYEEEHKRNLPRSLSAGGALDFGRKELEFVAEDSQEA 429

Query: 457 TKAAQEQAAKRIRYLEEELEKSRSEINLVRAERNKFELESGFAKEKLDSFMKEFEQQRVE 516
            KA QEQAAKRIRYLEEELEKSRSE+N VRAERNKFELE GFAKEKLDSFMKEFEQQRVE
Sbjct: 430 RKADQEQAAKRIRYLEEELEKSRSEVNFVRAERNKFELELGFAKEKLDSFMKEFEQQRVE 489

Query: 517 MNGVLARNVEFSQLIVDYQRKLREVSESLHSADEHSRKLTIEVSILKSEKELLSNAEKRA 576
           MNGVLARNVEFSQLIVDYQRKLREVSESLH+ADE+SRKL+IEVS++KSEKELLSNAEKRA
Sbjct: 490 MNGVLARNVEFSQLIVDYQRKLREVSESLHNADENSRKLSIEVSVIKSEKELLSNAEKRA 549

Query: 577 QDEIQNLSERLFRVQASLDTIRSVEEVHEEARVVERRKLEEHAKQLEGASAFSSLRKVKI 636
           QDEI+NLSERLFRVQ SLDTIRSVEEVHEE RVVERRKLEEHAKQLE             
Sbjct: 550 QDEIRNLSERLFRVQTSLDTIRSVEEVHEETRVVERRKLEEHAKQLE------------- 609

Query: 637 PKKVSRSGVFFVGVKRDILGIAHISYVSYDILYHFHVYDKLYMKREWAEAKKELQEERDN 696
                                                       REWAEAKKELQEERDN
Sbjct: 610 --------------------------------------------REWAEAKKELQEERDN 669

Query: 697 VRTLTLDREKTLKNAMSHVEEMGKELANALHATAAAEARAAVAEAKLSDLAKKISSSESQ 756
           VRTLTLDREKTLKNAMSHVEEMGKELANALHATA AEARAAVAEAKLSDL KKI++S++Q
Sbjct: 670 VRTLTLDREKTLKNAMSHVEEMGKELANALHATATAEARAAVAEAKLSDLEKKINASDNQ 729

Query: 757 VVELDDSSGLKSQPSDQVGTVLRSAESEIQKFKEEAQACKDHMLQYKSIAQVNEEAVRQM 816
           VVELDD SGLKSQPS+QV T LR AE+EIQKFKEEAQACKDHMLQYKSIAQVNEEAV+QM
Sbjct: 730 VVELDDRSGLKSQPSNQVATDLRRAEAEIQKFKEEAQACKDHMLQYKSIAQVNEEAVKQM 789

Query: 817 ECAHETFKKEAEKMKKSLEVELLQLRERVSELENESILKSQEIASAASLKEEAIASSLAD 876
           ECAHETFK EAEKMKKSLE ELLQLR+RV+ELENESILKSQEIASAASLKEEAIASSLAD
Sbjct: 790 ECAHETFKIEAEKMKKSLEDELLQLRQRVAELENESILKSQEIASAASLKEEAIASSLAD 837

Query: 877 VRNLKEENAAKTSKIQEMEIKISYLNEDLEREQQRWRTAQANYERQV 924
           +R L EENAAKTSKIQEMEI+ISYL EDLEREQQ+WRTAQANYERQV
Sbjct: 850 IRALNEENAAKTSKIQEMEIQISYLKEDLEREQQKWRTAQANYERQV 837

BLAST of Spg021421 vs. NCBI nr
Match: XP_038887699.1 (nuclear-pore anchor isoform X1 [Benincasa hispida])

HSP 1 Score: 1009.6 bits (2609), Expect = 1.8e-290
Identity = 581/707 (82.18%), Postives = 611/707 (86.42%), Query Frame = 0

Query: 217  IIVRQEDLCEEAMEEENTAEHERMIESYSLLNQKLQNSISEQAILEKTVQELKADLKRHE 276
            ++   ED  E  +EE   AEHERMIESYSLLNQKLQNSISEQAILEKT+QELKADLKRHE
Sbjct: 441  VLYELEDKAEVILEER--AEHERMIESYSLLNQKLQNSISEQAILEKTLQELKADLKRHE 500

Query: 277  RDYTLIQRENIDLSRQVTILLKECRDVQLRCGYTGHDVLENVSNTTSFEINVGSDADKVI 336
            RDY LIQRENIDLSRQ+TILLKECRDVQLRCGY GHDVLEN+S TTS EINV SDAD+VI
Sbjct: 501  RDYLLIQRENIDLSRQITILLKECRDVQLRCGYAGHDVLENLSKTTSLEINVESDADRVI 560

Query: 337  SEYLLTFKDINGLVEQNVQLRSLVRKLSVQLQDTELDFKEKLEVELKRQTQEAASRVEAV 396
            SEYLLTFKDINGLVEQNVQLRSLVRKLSVQLQD ELDFKE LEVELKR+TQEAASRVEAV
Sbjct: 561  SEYLLTFKDINGLVEQNVQLRSLVRKLSVQLQDAELDFKENLEVELKRKTQEAASRVEAV 620

Query: 397  LQKVEEQGQMIESLHASVAMYKRLYEEEHKRNLPHPLSAGVALGFGRKEQEFVSEDSQEA 456
            L+KVEEQGQMIESLHASV+MYKRLYEEEHKRNLP  LSAG AL FGRKE EFV+EDSQEA
Sbjct: 621  LKKVEEQGQMIESLHASVSMYKRLYEEEHKRNLPRSLSAGGALDFGRKELEFVAEDSQEA 680

Query: 457  TKAAQEQAAKRIRYLEEELEKSRSEINLVRAERNKFELESGFAKEKLDSFMKEFEQQRVE 516
             KA QEQAAKRIRYLEEELEKSRSE+N VRAERNKFELE GFAKEKLDSFMKEFEQQRVE
Sbjct: 681  RKADQEQAAKRIRYLEEELEKSRSEVNFVRAERNKFELELGFAKEKLDSFMKEFEQQRVE 740

Query: 517  MNGVLARNVEFSQLIVDYQRKLREVSESLHSADEHSRKLTIEVSILKSEKELLSNAEKRA 576
            MNGVLARNVEFSQLIVDYQRKLREVSESLH+ADE+SRKL+IEVS++KSEKELLSNAEKRA
Sbjct: 741  MNGVLARNVEFSQLIVDYQRKLREVSESLHNADENSRKLSIEVSVIKSEKELLSNAEKRA 800

Query: 577  QDEIQNLSERLFRVQASLDTIRSVEEVHEEARVVERRKLEEHAKQLEGASAFSSLRKVKI 636
            QDEI+NLSERLFRVQ SLDTIRSVEEVHEE RVVERRKLEEHAKQLE             
Sbjct: 801  QDEIRNLSERLFRVQTSLDTIRSVEEVHEETRVVERRKLEEHAKQLE------------- 860

Query: 637  PKKVSRSGVFFVGVKRDILGIAHISYVSYDILYHFHVYDKLYMKREWAEAKKELQEERDN 696
                                                        REWAEAKKELQEERDN
Sbjct: 861  --------------------------------------------REWAEAKKELQEERDN 920

Query: 697  VRTLTLDREKTLKNAMSHVEEMGKELANALHATAAAEARAAVAEAKLSDLAKKISSSESQ 756
            VRTLTLDREKTLKNAMSHVEEMGKELANALHATA AEARAAVAEAKLSDL KKI++S++Q
Sbjct: 921  VRTLTLDREKTLKNAMSHVEEMGKELANALHATATAEARAAVAEAKLSDLEKKINASDNQ 980

Query: 757  VVELDDSSGLKSQPSDQVGTVLRSAESEIQKFKEEAQACKDHMLQYKSIAQVNEEAVRQM 816
            VVELDD SGLKSQPS+QV T LR AE+EIQKFKEEAQACKDHMLQYKSIAQVNEEAV+QM
Sbjct: 981  VVELDDRSGLKSQPSNQVATDLRRAEAEIQKFKEEAQACKDHMLQYKSIAQVNEEAVKQM 1040

Query: 817  ECAHETFKKEAEKMKKSLEVELLQLRERVSELENESILKSQEIASAASLKEEAIASSLAD 876
            ECAHETFK EAEKMKKSLE ELLQLR+RV+ELENESILKSQEIASAASLKEEAIASSLAD
Sbjct: 1041 ECAHETFKIEAEKMKKSLEDELLQLRQRVAELENESILKSQEIASAASLKEEAIASSLAD 1088

Query: 877  VRNLKEENAAKTSKIQEMEIKISYLNEDLEREQQRWRTAQANYERQV 924
            +R L EENAAKTSKIQEMEI+ISYL EDLEREQQ+WRTAQANYERQV
Sbjct: 1101 IRALNEENAAKTSKIQEMEIQISYLKEDLEREQQKWRTAQANYERQV 1088

BLAST of Spg021421 vs. NCBI nr
Match: XP_011659057.1 (nuclear-pore anchor isoform X2 [Cucumis sativus] >KAE8646121.1 hypothetical protein Csa_016255 [Cucumis sativus])

HSP 1 Score: 997.3 bits (2577), Expect = 9.3e-287
Identity = 574/707 (81.19%), Postives = 606/707 (85.71%), Query Frame = 0

Query: 217  IIVRQEDLCEEAMEEENTAEHERMIESYSLLNQKLQNSISEQAILEKTVQELKADLKRHE 276
            ++   ED  E  +EE   AEHERMIESYSLLNQKLQNSISEQ ILEKT+QELKADLKRHE
Sbjct: 446  VLYELEDKAEVILEER--AEHERMIESYSLLNQKLQNSISEQEILEKTLQELKADLKRHE 505

Query: 277  RDYTLIQRENIDLSRQVTILLKECRDVQLRCGYTGHDVLENVSNTTSFEINVGSDADKVI 336
            RDY LI RENIDLSRQVTILLKECRDVQLRCGY G+DV +N+SN TSFEIN+ SDAD+VI
Sbjct: 506  RDYLLIHRENIDLSRQVTILLKECRDVQLRCGYVGNDVPKNISNPTSFEINMESDADRVI 565

Query: 337  SEYLLTFKDINGLVEQNVQLRSLVRKLSVQLQDTELDFKEKLEVELKRQTQEAASRVEAV 396
            SEYLLTFKDINGLVEQNVQLRSLVRKLSVQLQDTELDFKEKLE ELKR+TQEAASRVEAV
Sbjct: 566  SEYLLTFKDINGLVEQNVQLRSLVRKLSVQLQDTELDFKEKLEAELKRKTQEAASRVEAV 625

Query: 397  LQKVEEQGQMIESLHASVAMYKRLYEEEHKRNLPHPLSAGVALGFGRKEQEFVSEDSQEA 456
            LQKVEEQGQMIESLHASVAMYKRLYEEEHKRNL  PLSAGVAL FGRKE EFVS+DSQEA
Sbjct: 626  LQKVEEQGQMIESLHASVAMYKRLYEEEHKRNLHLPLSAGVALDFGRKELEFVSKDSQEA 685

Query: 457  TKAAQEQAAKRIRYLEEELEKSRSEINLVRAERNKFELESGFAKEKLDSFMKEFEQQRVE 516
             KA  EQAAKRIRYLEEELEKSRSE+N VRAERNKFELE GFAKEKLDSFMKEFEQQRVE
Sbjct: 686  RKADHEQAAKRIRYLEEELEKSRSEVNFVRAERNKFELEIGFAKEKLDSFMKEFEQQRVE 745

Query: 517  MNGVLARNVEFSQLIVDYQRKLREVSESLHSADEHSRKLTIEVSILKSEKELLSNAEKRA 576
            MNGVLARNVEFSQLIVDYQRKLREVSESLHSADE SRKL+IEVS+LKSEK+LLSNAEKRA
Sbjct: 746  MNGVLARNVEFSQLIVDYQRKLREVSESLHSADEQSRKLSIEVSVLKSEKDLLSNAEKRA 805

Query: 577  QDEIQNLSERLFRVQASLDTIRSVEEVHEEARVVERRKLEEHAKQLEGASAFSSLRKVKI 636
            QDEIQ LSERLFRVQ SLDTIRSVEEVHEE RVVERRKLEEHAKQLE             
Sbjct: 806  QDEIQKLSERLFRVQTSLDTIRSVEEVHEEVRVVERRKLEEHAKQLE------------- 865

Query: 637  PKKVSRSGVFFVGVKRDILGIAHISYVSYDILYHFHVYDKLYMKREWAEAKKELQEERDN 696
                                                        REWAEAKKELQEERDN
Sbjct: 866  --------------------------------------------REWAEAKKELQEERDN 925

Query: 697  VRTLTLDREKTLKNAMSHVEEMGKELANALHATAAAEARAAVAEAKLSDLAKKISSSESQ 756
            VRTLTLDREKTLKNAMSHVEEMGKELANALHATAAAEARAAVAEAKLSDL KKI +S++Q
Sbjct: 926  VRTLTLDREKTLKNAMSHVEEMGKELANALHATAAAEARAAVAEAKLSDLEKKICASDNQ 985

Query: 757  VVELDDSSGLKSQPSDQVGTVLRSAESEIQKFKEEAQACKDHMLQYKSIAQVNEEAVRQM 816
            V+ELDD S L S+P +QV T LR AE+EIQKFKEEAQACKDHMLQYKSIAQVNEEAV+QM
Sbjct: 986  VIELDDRSELSSRPPNQVATDLRRAEAEIQKFKEEAQACKDHMLQYKSIAQVNEEAVKQM 1045

Query: 817  ECAHETFKKEAEKMKKSLEVELLQLRERVSELENESILKSQEIASAASLKEEAIASSLAD 876
            ECAHETFK EAEKMKKSLEVELLQLRER++ELENES+LKSQEIASAASLKEEAIASSLA+
Sbjct: 1046 ECAHETFKIEAEKMKKSLEVELLQLRERIAELENESVLKSQEIASAASLKEEAIASSLAE 1093

Query: 877  VRNLKEENAAKTSKIQEMEIKISYLNEDLEREQQRWRTAQANYERQV 924
            ++NL EEN AKTSKIQEMEI+ISYL EDLER+QQ+WRTAQANYERQV
Sbjct: 1106 IKNLNEENTAKTSKIQEMEIQISYLKEDLERQQQKWRTAQANYERQV 1093

BLAST of Spg021421 vs. NCBI nr
Match: XP_011659056.1 (nuclear-pore anchor isoform X1 [Cucumis sativus])

HSP 1 Score: 997.3 bits (2577), Expect = 9.3e-287
Identity = 574/707 (81.19%), Postives = 606/707 (85.71%), Query Frame = 0

Query: 217  IIVRQEDLCEEAMEEENTAEHERMIESYSLLNQKLQNSISEQAILEKTVQELKADLKRHE 276
            ++   ED  E  +EE   AEHERMIESYSLLNQKLQNSISEQ ILEKT+QELKADLKRHE
Sbjct: 446  VLYELEDKAEVILEER--AEHERMIESYSLLNQKLQNSISEQEILEKTLQELKADLKRHE 505

Query: 277  RDYTLIQRENIDLSRQVTILLKECRDVQLRCGYTGHDVLENVSNTTSFEINVGSDADKVI 336
            RDY LI RENIDLSRQVTILLKECRDVQLRCGY G+DV +N+SN TSFEIN+ SDAD+VI
Sbjct: 506  RDYLLIHRENIDLSRQVTILLKECRDVQLRCGYVGNDVPKNISNPTSFEINMESDADRVI 565

Query: 337  SEYLLTFKDINGLVEQNVQLRSLVRKLSVQLQDTELDFKEKLEVELKRQTQEAASRVEAV 396
            SEYLLTFKDINGLVEQNVQLRSLVRKLSVQLQDTELDFKEKLE ELKR+TQEAASRVEAV
Sbjct: 566  SEYLLTFKDINGLVEQNVQLRSLVRKLSVQLQDTELDFKEKLEAELKRKTQEAASRVEAV 625

Query: 397  LQKVEEQGQMIESLHASVAMYKRLYEEEHKRNLPHPLSAGVALGFGRKEQEFVSEDSQEA 456
            LQKVEEQGQMIESLHASVAMYKRLYEEEHKRNL  PLSAGVAL FGRKE EFVS+DSQEA
Sbjct: 626  LQKVEEQGQMIESLHASVAMYKRLYEEEHKRNLHLPLSAGVALDFGRKELEFVSKDSQEA 685

Query: 457  TKAAQEQAAKRIRYLEEELEKSRSEINLVRAERNKFELESGFAKEKLDSFMKEFEQQRVE 516
             KA  EQAAKRIRYLEEELEKSRSE+N VRAERNKFELE GFAKEKLDSFMKEFEQQRVE
Sbjct: 686  RKADHEQAAKRIRYLEEELEKSRSEVNFVRAERNKFELEIGFAKEKLDSFMKEFEQQRVE 745

Query: 517  MNGVLARNVEFSQLIVDYQRKLREVSESLHSADEHSRKLTIEVSILKSEKELLSNAEKRA 576
            MNGVLARNVEFSQLIVDYQRKLREVSESLHSADE SRKL+IEVS+LKSEK+LLSNAEKRA
Sbjct: 746  MNGVLARNVEFSQLIVDYQRKLREVSESLHSADEQSRKLSIEVSVLKSEKDLLSNAEKRA 805

Query: 577  QDEIQNLSERLFRVQASLDTIRSVEEVHEEARVVERRKLEEHAKQLEGASAFSSLRKVKI 636
            QDEIQ LSERLFRVQ SLDTIRSVEEVHEE RVVERRKLEEHAKQLE             
Sbjct: 806  QDEIQKLSERLFRVQTSLDTIRSVEEVHEEVRVVERRKLEEHAKQLE------------- 865

Query: 637  PKKVSRSGVFFVGVKRDILGIAHISYVSYDILYHFHVYDKLYMKREWAEAKKELQEERDN 696
                                                        REWAEAKKELQEERDN
Sbjct: 866  --------------------------------------------REWAEAKKELQEERDN 925

Query: 697  VRTLTLDREKTLKNAMSHVEEMGKELANALHATAAAEARAAVAEAKLSDLAKKISSSESQ 756
            VRTLTLDREKTLKNAMSHVEEMGKELANALHATAAAEARAAVAEAKLSDL KKI +S++Q
Sbjct: 926  VRTLTLDREKTLKNAMSHVEEMGKELANALHATAAAEARAAVAEAKLSDLEKKICASDNQ 985

Query: 757  VVELDDSSGLKSQPSDQVGTVLRSAESEIQKFKEEAQACKDHMLQYKSIAQVNEEAVRQM 816
            V+ELDD S L S+P +QV T LR AE+EIQKFKEEAQACKDHMLQYKSIAQVNEEAV+QM
Sbjct: 986  VIELDDRSELSSRPPNQVATDLRRAEAEIQKFKEEAQACKDHMLQYKSIAQVNEEAVKQM 1045

Query: 817  ECAHETFKKEAEKMKKSLEVELLQLRERVSELENESILKSQEIASAASLKEEAIASSLAD 876
            ECAHETFK EAEKMKKSLEVELLQLRER++ELENES+LKSQEIASAASLKEEAIASSLA+
Sbjct: 1046 ECAHETFKIEAEKMKKSLEVELLQLRERIAELENESVLKSQEIASAASLKEEAIASSLAE 1093

Query: 877  VRNLKEENAAKTSKIQEMEIKISYLNEDLEREQQRWRTAQANYERQV 924
            ++NL EEN AKTSKIQEMEI+ISYL EDLER+QQ+WRTAQANYERQV
Sbjct: 1106 IKNLNEENTAKTSKIQEMEIQISYLKEDLERQQQKWRTAQANYERQV 1093

BLAST of Spg021421 vs. NCBI nr
Match: XP_008447306.1 (PREDICTED: nuclear-pore anchor-like [Cucumis melo])

HSP 1 Score: 990.7 bits (2560), Expect = 8.7e-285
Identity = 576/707 (81.47%), Postives = 603/707 (85.29%), Query Frame = 0

Query: 217 IIVRQEDLCEEAMEEENTAEHERMIESYSLLNQKLQNSISEQAILEKTVQELKADLKRHE 276
           ++   ED  E  +EE   AEHERMIESYSLLNQKLQNSISEQ ILEKT+QELKADLKRHE
Sbjct: 314 VLYELEDKAEVILEER--AEHERMIESYSLLNQKLQNSISEQEILEKTLQELKADLKRHE 373

Query: 277 RDYTLIQRENIDLSRQVTILLKECRDVQLRCGYTGHDVLENVSNTTSFEINVGSDADKVI 336
           RDY LI RENIDLSRQVTILLKECRDVQLRCGY G+DV EN+S  T FEIN+ SDAD+VI
Sbjct: 374 RDYLLIHRENIDLSRQVTILLKECRDVQLRCGYEGNDVPENLSIPTPFEINMESDADRVI 433

Query: 337 SEYLLTFKDINGLVEQNVQLRSLVRKLSVQLQDTELDFKEKLEVELKRQTQEAASRVEAV 396
           SEYLLTFKDINGLVEQNVQLRSLVRKLSVQLQDTELDFKEKLE ELKR+TQEAASRVEAV
Sbjct: 434 SEYLLTFKDINGLVEQNVQLRSLVRKLSVQLQDTELDFKEKLEAELKRKTQEAASRVEAV 493

Query: 397 LQKVEEQGQMIESLHASVAMYKRLYEEEHKRNLPHPLSAGVALGFGRKEQEFVSEDSQEA 456
           LQKVEEQGQMIESLHASVAMYKRLYEEEHKRNLP  LSAGVAL FGRKE EFVSEDSQEA
Sbjct: 494 LQKVEEQGQMIESLHASVAMYKRLYEEEHKRNLPLSLSAGVALDFGRKELEFVSEDSQEA 553

Query: 457 TKAAQEQAAKRIRYLEEELEKSRSEINLVRAERNKFELESGFAKEKLDSFMKEFEQQRVE 516
            KA Q+QAAKRIRYLEEELEKSRSE+N VRAE NKFELE GFAKEKLDSFMKEFEQQRVE
Sbjct: 554 RKADQKQAAKRIRYLEEELEKSRSEVNFVRAECNKFELEIGFAKEKLDSFMKEFEQQRVE 613

Query: 517 MNGVLARNVEFSQLIVDYQRKLREVSESLHSADEHSRKLTIEVSILKSEKELLSNAEKRA 576
           MNGVLARNVEFSQLIVDYQRKLREVSESLHSADE SRKL+IEVS+LKSEK+LLSNAEKRA
Sbjct: 614 MNGVLARNVEFSQLIVDYQRKLREVSESLHSADEQSRKLSIEVSVLKSEKDLLSNAEKRA 673

Query: 577 QDEIQNLSERLFRVQASLDTIRSVEEVHEEARVVERRKLEEHAKQLEGASAFSSLRKVKI 636
           QDEIQ LSERLFRVQASLDTIRSVEEVHEE RVVERRKLEEHAKQLE             
Sbjct: 674 QDEIQKLSERLFRVQASLDTIRSVEEVHEEVRVVERRKLEEHAKQLE------------- 733

Query: 637 PKKVSRSGVFFVGVKRDILGIAHISYVSYDILYHFHVYDKLYMKREWAEAKKELQEERDN 696
                                                       REWAEAKKELQEERDN
Sbjct: 734 --------------------------------------------REWAEAKKELQEERDN 793

Query: 697 VRTLTLDREKTLKNAMSHVEEMGKELANALHATAAAEARAAVAEAKLSDLAKKISSSESQ 756
           VRTLTLDREKTLKNAMSHVEEMGKELANALHATAAAEARAAVAEAKLSDL KKIS+S++Q
Sbjct: 794 VRTLTLDREKTLKNAMSHVEEMGKELANALHATAAAEARAAVAEAKLSDLEKKISASDNQ 853

Query: 757 VVELDDSSGLKSQPSDQVGTVLRSAESEIQKFKEEAQACKDHMLQYKSIAQVNEEAVRQM 816
           V+EL D S   S P +QV T LR AE+EIQKFKEEAQACKDHMLQYKSIAQVNEEAV+QM
Sbjct: 854 VIELVDRSEPSSHPPNQVATDLRRAEAEIQKFKEEAQACKDHMLQYKSIAQVNEEAVKQM 913

Query: 817 ECAHETFKKEAEKMKKSLEVELLQLRERVSELENESILKSQEIASAASLKEEAIASSLAD 876
           ECAHETFK EAEKMKKSLE ELLQLRERV+ELENESILKSQEIASAASLKEEAIASSLAD
Sbjct: 914 ECAHETFKIEAEKMKKSLEAELLQLRERVAELENESILKSQEIASAASLKEEAIASSLAD 961

Query: 877 VRNLKEENAAKTSKIQEMEIKISYLNEDLEREQQRWRTAQANYERQV 924
           +RNL EENAAKT+KIQEMEI+ISYL EDLER+QQ+WRTAQANYERQV
Sbjct: 974 IRNLNEENAAKTAKIQEMEIQISYLKEDLERQQQKWRTAQANYERQV 961

BLAST of Spg021421 vs. ExPASy Swiss-Prot
Match: A4GSN8 (Nuclear-pore anchor OS=Arabidopsis thaliana OX=3702 GN=NUA PE=1 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 1.6e-180
Identity = 383/727 (52.68%), Postives = 505/727 (69.46%), Query Frame = 0

Query: 231  EENTAEHERMIESYSLLNQKLQNSISEQAILEKTVQELKADLKRHERDYTLIQRENIDLS 290
            +E   E+ER++E+Y L+NQKLQ+S+SEQ+ +EK + ELKADL+R ER+ TL+Q++  DL 
Sbjct: 456  QEERGEYERVVEAYCLVNQKLQDSVSEQSNMEKFIMELKADLRRRERENTLLQKDISDLQ 515

Query: 291  RQVTILLKECRDVQLRCGYTGHDVLENVSNTTSFEINVGSDADKVISEYLLTFKDINGLV 350
            +QVTILLKECRDVQLRCG    D  ++    +  E+ + S+ADK+ISE+LL FKDINGLV
Sbjct: 516  KQVTILLKECRDVQLRCGAARDDDEDDYPLLSDVEMEMESEADKIISEHLLKFKDINGLV 575

Query: 351  EQNVQLRSLVRKLSVQLQDTELDFKEKLEVELKRQTQEAASRVEAVLQKVEEQGQMIESL 410
            EQNV+LR+LVR LS Q++  E + KE  EV+LK +T EA+++V  VL++ EEQGQMIESL
Sbjct: 576  EQNVKLRNLVRSLSEQIESRETELKETFEVDLKNKTDEASAKVATVLKRAEEQGQMIESL 635

Query: 411  HASVAMYKRLYEEEHKRNLPHPLSAGV--ALGFGRKEQEFVSEDSQEATKAAQEQAAKRI 470
            H SVAMYKRLYEEE K +     S+ +  A+  GRK    + EDS+EATK AQE+A +RI
Sbjct: 636  HTSVAMYKRLYEEEQKLHSSDSRSSDLSPAVVPGRKNFLHLLEDSEEATKRAQEKAFERI 695

Query: 471  RYLEEELEKSRSEINLVRAERNKFELESGFAKEKLDSFMKEFEQQRVEMNGVLARNVEFS 530
            R LEE+  K+RSE+  +R+ER+K  +E+ FA+EKL+  MKE E++R EMN VLARN+EFS
Sbjct: 696  RILEEDFAKARSEVIAIRSERDKLAMEANFAREKLEGIMKESERKREEMNSVLARNIEFS 755

Query: 531  QLIVDYQRKLREVSESLHSADEHSRKLTIEVSILKSEKELLSNAEKRAQDEIQNLSERLF 590
            QLI+D+QRKLRE SESLH+A+E SRKL++EVS+LK EKELLSNAEKRA DE+  LS+R++
Sbjct: 756  QLIIDHQRKLRESSESLHAAEEISRKLSMEVSVLKQEKELLSNAEKRASDEVSALSQRVY 815

Query: 591  RVQASLDTIRSVEEVHEEARVVERRKLEEHAKQLEGASAFSSLRKVKIPKKVSRSGVFFV 650
            R+QA+LDT++S EEV EE R  ERRK EEH KQL+                         
Sbjct: 816  RLQATLDTVQSTEEVREETRAAERRKQEEHIKQLQ------------------------- 875

Query: 651  GVKRDILGIAHISYVSYDILYHFHVYDKLYMKREWAEAKKELQEERDNVRTLTLDREKTL 710
                                            REWAEAKKELQEER N R  T DR +TL
Sbjct: 876  --------------------------------REWAEAKKELQEERSNARDFTSDRNQTL 935

Query: 711  KNAMSHVEEMGKELANALHATAAAEARAAVAEAKLSDLAKKISSSESQVVELDDSSGLKS 770
             NA+  VEEMGKELANAL A + AE+RA+VAEA+LSDL KKI SS+ + +++ DS G+ S
Sbjct: 936  NNAVMQVEEMGKELANALKAVSVAESRASVAEARLSDLEKKIRSSDPKTLDM-DSGGIVS 995

Query: 771  QPSDQVGTVLRSAESEIQKFKEEAQACKDHMLQYKSIAQVNEEAVRQMECAHETFKKEAE 830
                ++   LR+A+ EI+K + E ++ K HMLQYKSIAQVNE A++QME AHE F+ EAE
Sbjct: 996  LSDKEMSIELRTAKEEIEKLRGEVESSKSHMLQYKSIAQVNETALKQMESAHENFRLEAE 1055

Query: 831  KMKKSLEVELLQLRERVSELENESILKSQEIASAASLKEEAIASSLADVRNLKEENAAKT 890
            K ++SLE EL+ LRERVSELEN+ I KS+++A+AA+ KE+A+ S+ A++ +L+EEN  K 
Sbjct: 1056 KRQRSLEAELVSLRERVSELENDCIQKSEQLATAAAGKEDALLSASAEIASLREENLVKK 1115

Query: 891  SKIQEMEIKISYLNEDLEREQQRWRTAQANYERQV-----DGKDLINHPSCLCNLAENGS 950
            S+I+ M I++S L  DLE E ++WR AQ NYERQV       ++L      L  L E  S
Sbjct: 1116 SQIEAMNIQMSTLKNDLETEHEKWRVAQRNYERQVILLSETIQELTKTSQALAALQEEAS 1124

BLAST of Spg021421 vs. ExPASy Swiss-Prot
Match: Q5EE04 (Nucleoprotein TPR (Fragment) OS=Xenopus laevis OX=8355 GN=tpr PE=1 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 1.0e-06
Identity = 172/813 (21.16%), Postives = 339/813 (41.70%), Query Frame = 0

Query: 186 EVEDDLCVKPMENFELKSPQFEDLNPMSDTNIIVRQEDLCEEAMEEENTAEHERMIESYS 245
           E +D L ++  EN  +     E +  +   + I++++             E+ERM ++ +
Sbjct: 80  ETQDKLLMEKQENKRITKYLDEIVKEVEAKSPILKRQ-----------REEYERMQKTVA 139

Query: 246 LLNQKLQNSISEQAILEKTVQELKADLKRHERDYTLIQREN-------IDLSRQVTILLK 305
            L+ KL+ ++ E       +Q ++ +  +  +  ++++REN        DLS+Q+ +LL 
Sbjct: 140 SLSAKLEQAMRE-------IQRMQDETDKANKCSSVLERENQRLELQIKDLSQQIRVLLM 199

Query: 306 ECRDVQLRCGYTGHDVLENVSNTTSFEINVGSDADKVISEYLLTFKDINGLVEQNVQLRS 365
           E  +   R  +   D + + + ++S E         VI+++L+T+++I  L +QN +L  
Sbjct: 200 ELEEA--RGNFVQRDDVSSANISSSSE---------VITQHLVTYRNIEELQQQNQRLLV 259

Query: 366 LVRKLSVQLQDTELDFKEKLEVELKRQTQEAASRVEAVLQKVEEQGQMIESLHASVAMYK 425
            +R+L    +  E +       EL+++ + A S ++ + +    Q  ++ES+     MY+
Sbjct: 260 ALRELGEAKEREEQESTSSRVSELEKELENALSELQQLREARSHQMTLVESIVRQRDMYR 319

Query: 426 RLYEEEHKRNLPHP----------LSAGVAL-GFGRKEQEFVSEDSQEATKAA------- 485
            L  +     LP             S GV+L G        V  DS EA +A        
Sbjct: 320 ILLSQTTGVVLPAQDETALTSTPRKSPGVSLDGSTSTPAAVVVSDSTEAAEARAALKQLQ 379

Query: 486 ------QEQAAKRIRYLEEELEKSRSEINLVRAERNKFELESGFAKEKLDSFMKEFEQQR 545
                 +++ A+  R L E+ +K + ++  +R++  K   +  FA ++ +      E  R
Sbjct: 380 EVFENYRKEKAENDRMLNEQHDKLQEQVTELRSQNTKISTQLEFASKRYEMLQDNVEGYR 439

Query: 546 VEMNGVLARNVEFSQLIVDYQRKLREVSESLHSADEHSRKLTIEVSILKSEKELLSNAEK 605
            E+  +  +  + S      ++ +  ++  L +A+E      +    LK EKELL  +E 
Sbjct: 440 REITALQEKTQKLSATTQKQEQIINTLTHDLRAANEKLAVAEVRAENLKREKELLKMSEV 499

Query: 606 RAQDEIQNLSERLFRVQASLDTIRSVEEVHEEARVVERRKLEEHAKQLEGASAFSSLRKV 665
           R   E ++L          L  +++++   E +    +++     ++LE   A +   K 
Sbjct: 500 RLTQERESLVAEQRGQNLLLTNLQTIQVTLERSETEIKQRYNNQIEKLEQELAQT---KK 559

Query: 666 KIPKKVSRSGVFFVGVKRDILGIAHISYVSYDILYHFHVYDKLYMK---REWAEAKKELQ 725
           K+  ++ +     +G  +D+  +       Y++  + H   K  +K   +E +  K++L 
Sbjct: 560 KLEHEIEQR--HLLGKNQDVQVLE--LKKQYEMELNLHNNTKELLKNSHKEISVLKQQLN 619

Query: 726 -----------------------EERDNVRT-------LTLDREKTLKNAMSHVEE---- 785
                                  E+ + ++T       L  D ++ LK A S+VE+    
Sbjct: 620 SFELQLASRSSQQAANRDKDVNIEDVEEIKTKLRQSEELVNDLKERLKTATSNVEQYRSV 679

Query: 786 -------MGKELANALHATAAAEARAAVAEAKLSDLAKKISSSESQVVELDDSSGLKSQP 845
                  + KE           E R   +    S L KK+  SE +  EL D      + 
Sbjct: 680 VLNLEESLNKEKQVTEEVRKTIEVRLKESSEYQSQLEKKMMESEKEKQELRDEKHKTVEQ 739

Query: 846 SDQVGTVLRSAESEIQKFKEEAQACKDHMLQYKSIAQVNEEAVRQMEC-------AHETF 905
            +Q  T LR + S +Q   ++A       LQ  + +  NE+  +Q +C       A    
Sbjct: 740 MEQQVTQLRQSLSSLQAEVQQA-------LQRATTSASNEQKAKQ-DCQEQARIAAEAQN 799

Query: 906 KKEAEKMKKSLEVELLQLRERVSELENESILKSQEIA-SAASLKEEAIASSLADVRNLKE 916
           K E E M  + +VE LQ  ++     +    K +E A  A S   E+ AS     R LKE
Sbjct: 800 KYERELMLHAADVEALQAAKKQLTSASAIRHKCEETAQKAGSQLLESRASWEERERMLKE 848

BLAST of Spg021421 vs. ExPASy TrEMBL
Match: A0A1S3BI04 (nuclear-pore anchor-like OS=Cucumis melo OX=3656 GN=LOC103489776 PE=4 SV=1)

HSP 1 Score: 990.7 bits (2560), Expect = 4.2e-285
Identity = 576/707 (81.47%), Postives = 603/707 (85.29%), Query Frame = 0

Query: 217 IIVRQEDLCEEAMEEENTAEHERMIESYSLLNQKLQNSISEQAILEKTVQELKADLKRHE 276
           ++   ED  E  +EE   AEHERMIESYSLLNQKLQNSISEQ ILEKT+QELKADLKRHE
Sbjct: 314 VLYELEDKAEVILEER--AEHERMIESYSLLNQKLQNSISEQEILEKTLQELKADLKRHE 373

Query: 277 RDYTLIQRENIDLSRQVTILLKECRDVQLRCGYTGHDVLENVSNTTSFEINVGSDADKVI 336
           RDY LI RENIDLSRQVTILLKECRDVQLRCGY G+DV EN+S  T FEIN+ SDAD+VI
Sbjct: 374 RDYLLIHRENIDLSRQVTILLKECRDVQLRCGYEGNDVPENLSIPTPFEINMESDADRVI 433

Query: 337 SEYLLTFKDINGLVEQNVQLRSLVRKLSVQLQDTELDFKEKLEVELKRQTQEAASRVEAV 396
           SEYLLTFKDINGLVEQNVQLRSLVRKLSVQLQDTELDFKEKLE ELKR+TQEAASRVEAV
Sbjct: 434 SEYLLTFKDINGLVEQNVQLRSLVRKLSVQLQDTELDFKEKLEAELKRKTQEAASRVEAV 493

Query: 397 LQKVEEQGQMIESLHASVAMYKRLYEEEHKRNLPHPLSAGVALGFGRKEQEFVSEDSQEA 456
           LQKVEEQGQMIESLHASVAMYKRLYEEEHKRNLP  LSAGVAL FGRKE EFVSEDSQEA
Sbjct: 494 LQKVEEQGQMIESLHASVAMYKRLYEEEHKRNLPLSLSAGVALDFGRKELEFVSEDSQEA 553

Query: 457 TKAAQEQAAKRIRYLEEELEKSRSEINLVRAERNKFELESGFAKEKLDSFMKEFEQQRVE 516
            KA Q+QAAKRIRYLEEELEKSRSE+N VRAE NKFELE GFAKEKLDSFMKEFEQQRVE
Sbjct: 554 RKADQKQAAKRIRYLEEELEKSRSEVNFVRAECNKFELEIGFAKEKLDSFMKEFEQQRVE 613

Query: 517 MNGVLARNVEFSQLIVDYQRKLREVSESLHSADEHSRKLTIEVSILKSEKELLSNAEKRA 576
           MNGVLARNVEFSQLIVDYQRKLREVSESLHSADE SRKL+IEVS+LKSEK+LLSNAEKRA
Sbjct: 614 MNGVLARNVEFSQLIVDYQRKLREVSESLHSADEQSRKLSIEVSVLKSEKDLLSNAEKRA 673

Query: 577 QDEIQNLSERLFRVQASLDTIRSVEEVHEEARVVERRKLEEHAKQLEGASAFSSLRKVKI 636
           QDEIQ LSERLFRVQASLDTIRSVEEVHEE RVVERRKLEEHAKQLE             
Sbjct: 674 QDEIQKLSERLFRVQASLDTIRSVEEVHEEVRVVERRKLEEHAKQLE------------- 733

Query: 637 PKKVSRSGVFFVGVKRDILGIAHISYVSYDILYHFHVYDKLYMKREWAEAKKELQEERDN 696
                                                       REWAEAKKELQEERDN
Sbjct: 734 --------------------------------------------REWAEAKKELQEERDN 793

Query: 697 VRTLTLDREKTLKNAMSHVEEMGKELANALHATAAAEARAAVAEAKLSDLAKKISSSESQ 756
           VRTLTLDREKTLKNAMSHVEEMGKELANALHATAAAEARAAVAEAKLSDL KKIS+S++Q
Sbjct: 794 VRTLTLDREKTLKNAMSHVEEMGKELANALHATAAAEARAAVAEAKLSDLEKKISASDNQ 853

Query: 757 VVELDDSSGLKSQPSDQVGTVLRSAESEIQKFKEEAQACKDHMLQYKSIAQVNEEAVRQM 816
           V+EL D S   S P +QV T LR AE+EIQKFKEEAQACKDHMLQYKSIAQVNEEAV+QM
Sbjct: 854 VIELVDRSEPSSHPPNQVATDLRRAEAEIQKFKEEAQACKDHMLQYKSIAQVNEEAVKQM 913

Query: 817 ECAHETFKKEAEKMKKSLEVELLQLRERVSELENESILKSQEIASAASLKEEAIASSLAD 876
           ECAHETFK EAEKMKKSLE ELLQLRERV+ELENESILKSQEIASAASLKEEAIASSLAD
Sbjct: 914 ECAHETFKIEAEKMKKSLEAELLQLRERVAELENESILKSQEIASAASLKEEAIASSLAD 961

Query: 877 VRNLKEENAAKTSKIQEMEIKISYLNEDLEREQQRWRTAQANYERQV 924
           +RNL EENAAKT+KIQEMEI+ISYL EDLER+QQ+WRTAQANYERQV
Sbjct: 974 IRNLNEENAAKTAKIQEMEIQISYLKEDLERQQQKWRTAQANYERQV 961

BLAST of Spg021421 vs. ExPASy TrEMBL
Match: A0A5A7V762 (Nuclear-pore anchor isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold357G00370 PE=4 SV=1)

HSP 1 Score: 983.8 bits (2542), Expect = 5.1e-283
Identity = 575/708 (81.21%), Postives = 602/708 (85.03%), Query Frame = 0

Query: 217  IIVRQEDLCEEAMEEENTAEHERMIESYSLLNQKLQNSISEQAILEKTVQELKADLKRHE 276
            ++   ED  E  +EE   AEHERMIESYSLLNQKLQNSISEQ ILEKT+QELKADLKRHE
Sbjct: 446  VLYELEDKAEVILEER--AEHERMIESYSLLNQKLQNSISEQEILEKTLQELKADLKRHE 505

Query: 277  RDYTLIQRENIDLSRQVTILLKECRDVQLRCGYTGHDVLENVSNTTSFEINVGSDADKVI 336
            RDY LI RENIDLSRQVTILLKECRDVQLRCGY G+DV EN+S  T FEIN+ SDAD+VI
Sbjct: 506  RDYLLIHRENIDLSRQVTILLKECRDVQLRCGYEGNDVPENLSKPTPFEINMESDADRVI 565

Query: 337  SEYLLTFKDINGLVEQNVQLRSLVRKLSVQLQDTELDFKEKLEVELKRQTQEAASRVEAV 396
            SEYLLTFKDINGLVEQNVQLRSLVRKLSVQLQDTELDFKEKLE ELKR+TQEAASRVEAV
Sbjct: 566  SEYLLTFKDINGLVEQNVQLRSLVRKLSVQLQDTELDFKEKLEAELKRKTQEAASRVEAV 625

Query: 397  LQKVEEQGQMIESLHASVAMYKRLYEEEHKRNLPHPLSAGVALGFGRKEQEFVSEDSQEA 456
            LQKVEEQGQMIESLHASVAMYKRLYEEEHKRNLP  LSAGVAL FGRKE EFVSEDSQEA
Sbjct: 626  LQKVEEQGQMIESLHASVAMYKRLYEEEHKRNLPLSLSAGVALDFGRKELEFVSEDSQEA 685

Query: 457  TKAAQEQAAKRIRYLEEELEKSRSEINLVRAERNKFELESGFAKEKLDSFMKEFEQQRVE 516
             KA Q+QAAKRIR LEEELEKSRSE+N VRAE NKFELE GFAKEKLDSFMKEFEQQRVE
Sbjct: 686  RKADQKQAAKRIRNLEEELEKSRSEVNFVRAECNKFELEIGFAKEKLDSFMKEFEQQRVE 745

Query: 517  MNGVLARNVEFSQLIVDYQRKLREVSESLHSADEHSRKLTIEVSILKSEKELLSNAEKRA 576
            MNGVLARNVEFSQLIVDYQRKLREVSESLHSADE SRKL+IEVS+LKSEK+LLSNAEKRA
Sbjct: 746  MNGVLARNVEFSQLIVDYQRKLREVSESLHSADEQSRKLSIEVSVLKSEKDLLSNAEKRA 805

Query: 577  QDEIQNLSERLFRVQASLDTIRSVEEVHEEARVVERRKLEEHAKQLEGASAFSSLRKVKI 636
            QDEIQ LSERLFRVQASLDTIRSVEEVHEE RVVERRKLEEHAKQLE             
Sbjct: 806  QDEIQKLSERLFRVQASLDTIRSVEEVHEEVRVVERRKLEEHAKQLE------------- 865

Query: 637  PKKVSRSGVFFVGVKRDILGIAHISYVSYDILYHFHVYDKLYMKREWAEAKKELQEERDN 696
                                                        REWAEAKKELQEERDN
Sbjct: 866  --------------------------------------------REWAEAKKELQEERDN 925

Query: 697  VRTLTLDREKTLKNAMSHVEEMGKELANALHATAAAEARAAVAEAKLSDLAKKISSSESQ 756
            VRTLTLDREKTLKNAMSHVEEMGKELANALHATAAAEARAAVAEAKLSDL KKIS+S++Q
Sbjct: 926  VRTLTLDREKTLKNAMSHVEEMGKELANALHATAAAEARAAVAEAKLSDLEKKISASDNQ 985

Query: 757  VVELDDSSGLKSQPSDQ-VGTVLRSAESEIQKFKEEAQACKDHMLQYKSIAQVNEEAVRQ 816
            V+EL D S   S P +Q V T LR AE+EIQKFKEEAQACKDHMLQYKSIAQVNEEAV+Q
Sbjct: 986  VIELVDRSEPSSHPPNQVVATDLRRAEAEIQKFKEEAQACKDHMLQYKSIAQVNEEAVKQ 1045

Query: 817  MECAHETFKKEAEKMKKSLEVELLQLRERVSELENESILKSQEIASAASLKEEAIASSLA 876
            MECAHETFK EAEKMKKSLE ELLQLRERV+ELENESILKSQEIASAASLKEEAIASSLA
Sbjct: 1046 MECAHETFKIEAEKMKKSLEAELLQLRERVAELENESILKSQEIASAASLKEEAIASSLA 1094

Query: 877  DVRNLKEENAAKTSKIQEMEIKISYLNEDLEREQQRWRTAQANYERQV 924
            D+RNL EENAAKT+KIQEMEI+ISYL EDLER+QQ+WRTAQANYERQV
Sbjct: 1106 DIRNLNEENAAKTAKIQEMEIQISYLKEDLERQQQKWRTAQANYERQV 1094

BLAST of Spg021421 vs. ExPASy TrEMBL
Match: A0A6J1I2E6 (nuclear-pore anchor isoform X4 OS=Cucurbita maxima OX=3661 GN=LOC111468397 PE=4 SV=1)

HSP 1 Score: 980.7 bits (2534), Expect = 4.3e-282
Identity = 578/742 (77.90%), Postives = 614/742 (82.75%), Query Frame = 0

Query: 217  IIVRQEDLCEEAMEEENTAEHERMIESYSLLNQKLQNSISEQAILEKTVQELKADLKRHE 276
            ++   ED  E  +EE   AEHE MIESYSLLNQKLQ+SISEQAILEK+VQELKA LKRHE
Sbjct: 446  VLCELEDKAEVILEER--AEHEGMIESYSLLNQKLQHSISEQAILEKSVQELKAVLKRHE 505

Query: 277  RDYTLIQRENIDLSRQVTILLKECRDVQLRCGYTGHDVLENVSNTTSFEINVGSDADKVI 336
            RDY LIQREN+DLSRQVTILLKECRDVQLRCG  GHDVLEN+S  TSF+INV SDAD+VI
Sbjct: 506  RDYMLIQRENVDLSRQVTILLKECRDVQLRCGSAGHDVLENLSTATSFQINVESDADRVI 565

Query: 337  SEYLLTFKDINGLVEQNVQLRSLVRKLSVQLQDTELDFKEKLEVELKRQTQEAASRVEAV 396
            SEYLLTFKDINGLVEQNVQLRSLVRKLSVQLQDTELDFKEKLE ELK +TQEAASRVEAV
Sbjct: 566  SEYLLTFKDINGLVEQNVQLRSLVRKLSVQLQDTELDFKEKLEAELKSKTQEAASRVEAV 625

Query: 397  LQKVEEQGQMIESLHASVAMYKRLYEEEHKRNLPHPLSAGVALGFGRKEQEFVSEDSQEA 456
            LQKVEEQGQMIESLHASVAMYKRLYEEE KR+LPH  SAGVAL F RKE +FVSEDSQE 
Sbjct: 626  LQKVEEQGQMIESLHASVAMYKRLYEEEQKRSLPHSHSAGVALDFCRKELKFVSEDSQED 685

Query: 457  TKAAQEQAAKRIRYLEEELEKSRSEINLVRAERNKFELESGFAKEKLDSFMKEFEQQRVE 516
            TKAA +QA+KR+R LEEELEKSRSEINLVRAERNKFELESGFAKEKL SFMKEFEQQRVE
Sbjct: 686  TKAALDQASKRVRSLEEELEKSRSEINLVRAERNKFELESGFAKEKLASFMKEFEQQRVE 745

Query: 517  MNGVLARNVEFSQLIVDYQRKLREVSESLHSADEHSRKLTIEVSILKSEKELLSNAEKRA 576
            MNGVLARNVEFSQL+VDYQ KLR+VSESLHSADE SRKL+IEVSILKSEKELLSNAEKRA
Sbjct: 746  MNGVLARNVEFSQLLVDYQGKLRKVSESLHSADEQSRKLSIEVSILKSEKELLSNAEKRA 805

Query: 577  QDEIQNLSERLFRVQASLDTIRSVEEVHEEARVVERRKLEEHAKQLEGASAFSSLRKVKI 636
             DEI+NLSERLFRVQASLDTIRSVEEVHEEARVVERRKLEEHAKQLE             
Sbjct: 806  HDEIRNLSERLFRVQASLDTIRSVEEVHEEARVVERRKLEEHAKQLE------------- 865

Query: 637  PKKVSRSGVFFVGVKRDILGIAHISYVSYDILYHFHVYDKLYMKREWAEAKKELQEERDN 696
                                                        REWAEAKKELQEERDN
Sbjct: 866  --------------------------------------------REWAEAKKELQEERDN 925

Query: 697  VRTLTLDREKTLKNAMSHVEEMGKELANALHATAAAEARAAVAEAKLSDLAKKISSSESQ 756
            VRTLTLDREKTLKNAMSHVE+MGKELANALHATAAAEARAAVAEAKL DL KKISSS +Q
Sbjct: 926  VRTLTLDREKTLKNAMSHVEDMGKELANALHATAAAEARAAVAEAKLFDLEKKISSSANQ 985

Query: 757  VVELDDSSGLKSQPSDQVGTVLRSAESEIQKFKEEAQACKDHMLQYKSIAQVNEEAVRQM 816
            V+ELDD SGLKS PS+QVG  L   E+EI+KFKEEAQACK+HMLQYKSIAQVNEEAV+QM
Sbjct: 986  VIELDDRSGLKSHPSNQVGIDLHRTEAEIKKFKEEAQACKEHMLQYKSIAQVNEEAVKQM 1045

Query: 817  ECAHETFKKEAEKMKKSLEVELLQLRERVSELENESILKSQEIASAASLKEEAIASSLAD 876
            ECAHE+FK EAEKMKKSLEVELLQLRERV+ELENESILKSQEIASAA LKEEAIASSLAD
Sbjct: 1046 ECAHESFKIEAEKMKKSLEVELLQLRERVAELENESILKSQEIASAARLKEEAIASSLAD 1105

Query: 877  VRNLKEENAAKTSKIQEMEIKISYLNEDLEREQQRWRTAQANYERQV-----DGKDLINH 936
            VRNL EENAAKTSKI EMEI+ISYL EDLEREQQ+WRTAQANYERQV       ++L   
Sbjct: 1106 VRNLNEENAAKTSKILEMEIQISYLKEDLEREQQKWRTAQANYERQVILQSETIQELTKT 1128

Query: 937  PSCLCNLAENGSKKRYFTDHLK 954
               L +L E  ++ R   D  K
Sbjct: 1166 SQALASLQEEAAELRKLADAYK 1128

BLAST of Spg021421 vs. ExPASy TrEMBL
Match: A0A6J1I0S6 (nuclear-pore anchor isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111468397 PE=4 SV=1)

HSP 1 Score: 976.5 bits (2523), Expect = 8.2e-281
Identity = 578/743 (77.79%), Postives = 614/743 (82.64%), Query Frame = 0

Query: 217  IIVRQEDLCEEAMEEENTAEHERMIESYSLLNQKLQNSISEQAILEKTVQELKADLKRHE 276
            ++   ED  E  +EE   AEHE MIESYSLLNQKLQ+SISEQAILEK+VQELKA LKRHE
Sbjct: 446  VLCELEDKAEVILEER--AEHEGMIESYSLLNQKLQHSISEQAILEKSVQELKAVLKRHE 505

Query: 277  RDYTLIQRENIDLSRQVTILLKECRDVQLRCGYTGHDVLENVSNTTSFEINVGSDADKVI 336
            RDY LIQREN+DLSRQVTILLKECRDVQLRCG  GHDVLEN+S  TSF+INV SDAD+VI
Sbjct: 506  RDYMLIQRENVDLSRQVTILLKECRDVQLRCGSAGHDVLENLSTATSFQINVESDADRVI 565

Query: 337  SEYLLTFKDINGLVEQNVQLRSLVRKLSVQLQDTELDFKEKLEVELKRQTQEAASRVEAV 396
            SEYLLTFKDINGLVEQNVQLRSLVRKLSVQLQDTELDFKEKLE ELK +TQEAASRVEAV
Sbjct: 566  SEYLLTFKDINGLVEQNVQLRSLVRKLSVQLQDTELDFKEKLEAELKSKTQEAASRVEAV 625

Query: 397  LQKVEEQGQMIESLHASVAMYKRLYEEEHKRNLPHPLSAGVALG-FGRKEQEFVSEDSQE 456
            LQKVEEQGQMIESLHASVAMYKRLYEEE KR+LPH  SAGVAL  F RKE +FVSEDSQE
Sbjct: 626  LQKVEEQGQMIESLHASVAMYKRLYEEEQKRSLPHSHSAGVALADFCRKELKFVSEDSQE 685

Query: 457  ATKAAQEQAAKRIRYLEEELEKSRSEINLVRAERNKFELESGFAKEKLDSFMKEFEQQRV 516
             TKAA +QA+KR+R LEEELEKSRSEINLVRAERNKFELESGFAKEKL SFMKEFEQQRV
Sbjct: 686  DTKAALDQASKRVRSLEEELEKSRSEINLVRAERNKFELESGFAKEKLASFMKEFEQQRV 745

Query: 517  EMNGVLARNVEFSQLIVDYQRKLREVSESLHSADEHSRKLTIEVSILKSEKELLSNAEKR 576
            EMNGVLARNVEFSQL+VDYQ KLR+VSESLHSADE SRKL+IEVSILKSEKELLSNAEKR
Sbjct: 746  EMNGVLARNVEFSQLLVDYQGKLRKVSESLHSADEQSRKLSIEVSILKSEKELLSNAEKR 805

Query: 577  AQDEIQNLSERLFRVQASLDTIRSVEEVHEEARVVERRKLEEHAKQLEGASAFSSLRKVK 636
            A DEI+NLSERLFRVQASLDTIRSVEEVHEEARVVERRKLEEHAKQLE            
Sbjct: 806  AHDEIRNLSERLFRVQASLDTIRSVEEVHEEARVVERRKLEEHAKQLE------------ 865

Query: 637  IPKKVSRSGVFFVGVKRDILGIAHISYVSYDILYHFHVYDKLYMKREWAEAKKELQEERD 696
                                                         REWAEAKKELQEERD
Sbjct: 866  ---------------------------------------------REWAEAKKELQEERD 925

Query: 697  NVRTLTLDREKTLKNAMSHVEEMGKELANALHATAAAEARAAVAEAKLSDLAKKISSSES 756
            NVRTLTLDREKTLKNAMSHVE+MGKELANALHATAAAEARAAVAEAKL DL KKISSS +
Sbjct: 926  NVRTLTLDREKTLKNAMSHVEDMGKELANALHATAAAEARAAVAEAKLFDLEKKISSSAN 985

Query: 757  QVVELDDSSGLKSQPSDQVGTVLRSAESEIQKFKEEAQACKDHMLQYKSIAQVNEEAVRQ 816
            QV+ELDD SGLKS PS+QVG  L   E+EI+KFKEEAQACK+HMLQYKSIAQVNEEAV+Q
Sbjct: 986  QVIELDDRSGLKSHPSNQVGIDLHRTEAEIKKFKEEAQACKEHMLQYKSIAQVNEEAVKQ 1045

Query: 817  MECAHETFKKEAEKMKKSLEVELLQLRERVSELENESILKSQEIASAASLKEEAIASSLA 876
            MECAHE+FK EAEKMKKSLEVELLQLRERV+ELENESILKSQEIASAA LKEEAIASSLA
Sbjct: 1046 MECAHESFKIEAEKMKKSLEVELLQLRERVAELENESILKSQEIASAARLKEEAIASSLA 1105

Query: 877  DVRNLKEENAAKTSKIQEMEIKISYLNEDLEREQQRWRTAQANYERQV-----DGKDLIN 936
            DVRNL EENAAKTSKI EMEI+ISYL EDLEREQQ+WRTAQANYERQV       ++L  
Sbjct: 1106 DVRNLNEENAAKTSKILEMEIQISYLKEDLEREQQKWRTAQANYERQVILQSETIQELTK 1129

Query: 937  HPSCLCNLAENGSKKRYFTDHLK 954
                L +L E  ++ R   D  K
Sbjct: 1166 TSQALASLQEEAAELRKLADAYK 1129

BLAST of Spg021421 vs. ExPASy TrEMBL
Match: A0A6J1CLF4 (nuclear-pore anchor isoform X2 OS=Momordica charantia OX=3673 GN=LOC111012354 PE=4 SV=1)

HSP 1 Score: 976.5 bits (2523), Expect = 8.2e-281
Identity = 561/742 (75.61%), Postives = 616/742 (83.02%), Query Frame = 0

Query: 217  IIVRQEDLCEEAMEEENTAEHERMIESYSLLNQKLQNSISEQAILEKTVQELKADLKRHE 276
            ++   ED  E  +EE   AEHERMIESYSLLNQKLQNSISEQAILEKT++ELKADLK HE
Sbjct: 446  VLYELEDKAEVILEER--AEHERMIESYSLLNQKLQNSISEQAILEKTIKELKADLKIHE 505

Query: 277  RDYTLIQRENIDLSRQVTILLKECRDVQLRCGYTGHDVLENVSNTTSFEINVGSDADKVI 336
            RDY LIQREN+DLS+QV+ILLKECRDVQLRCGYTGHDV EN+SNTTSF++NV SDAD+VI
Sbjct: 506  RDYMLIQRENVDLSKQVSILLKECRDVQLRCGYTGHDVHENLSNTTSFDMNVESDADRVI 565

Query: 337  SEYLLTFKDINGLVEQNVQLRSLVRKLSVQLQDTELDFKEKLEVELKRQTQEAASRVEAV 396
            SEYLLTFKDINGLVEQNVQLRSLVRKLSVQ+QD+ELDFKEK+EVELKR TQEAAS+VEAV
Sbjct: 566  SEYLLTFKDINGLVEQNVQLRSLVRKLSVQIQDSELDFKEKMEVELKRHTQEAASKVEAV 625

Query: 397  LQKVEEQGQMIESLHASVAMYKRLYEEEHKRNLPHPLSAGVALGFGRKEQEFVSEDSQEA 456
            LQKVEEQGQMIESLHASVAMYKRLYEEEHKRN+ HPLSA VAL  GR+E E VSEDSQE 
Sbjct: 626  LQKVEEQGQMIESLHASVAMYKRLYEEEHKRNVSHPLSAEVALDSGRRELEIVSEDSQEG 685

Query: 457  TKAAQEQAAKRIRYLEEELEKSRSEINLVRAERNKFELESGFAKEKLDSFMKEFEQQRVE 516
            TKAAQ+Q AKR+RYLEEELEKSRSEINLVRAERNKFEL S F KEKLDSFMKEFEQQRVE
Sbjct: 686  TKAAQKQTAKRVRYLEEELEKSRSEINLVRAERNKFELASSFTKEKLDSFMKEFEQQRVE 745

Query: 517  MNGVLARNVEFSQLIVDYQRKLREVSESLHSADEHSRKLTIEVSILKSEKELLSNAEKRA 576
            MNGVLARNVEFSQLIVDYQRKLREVSESL SADE SRKLT+EVS+LK+EKELLSNAEKRA
Sbjct: 746  MNGVLARNVEFSQLIVDYQRKLREVSESLQSADEQSRKLTMEVSVLKTEKELLSNAEKRA 805

Query: 577  QDEIQNLSERLFRVQASLDTIRSVEEVHEEARVVERRKLEEHAKQLEGASAFSSLRKVKI 636
            QDE++NLSERLFRVQASLDTI+SVEEVHEEARV ERRKLEEHAKQLE             
Sbjct: 806  QDEVRNLSERLFRVQASLDTIQSVEEVHEEARVAERRKLEEHAKQLE------------- 865

Query: 637  PKKVSRSGVFFVGVKRDILGIAHISYVSYDILYHFHVYDKLYMKREWAEAKKELQEERDN 696
                                                        REWAE KKELQEERDN
Sbjct: 866  --------------------------------------------REWAELKKELQEERDN 925

Query: 697  VRTLTLDREKTLKNAMSHVEEMGKELANALHATAAAEARAAVAEAKLSDLAKKISSSESQ 756
            VRTLTLDREKTLKNAM HVEEMGKEL+NALHATAAAEARAA++EAKLS+L KKISSS++Q
Sbjct: 926  VRTLTLDREKTLKNAMGHVEEMGKELSNALHATAAAEARAAISEAKLSELEKKISSSDNQ 985

Query: 757  VVELDDSSGLKSQPSDQVGTVLRSAESEIQKFKEEAQACKDHMLQYKSIAQVNEEAVRQM 816
            VV+LD+ SGLKSQPS+QVGT L  AE +IQKFKEEAQACKDHMLQYK+IAQVNEEA++QM
Sbjct: 986  VVDLDERSGLKSQPSNQVGTDLHRAEEQIQKFKEEAQACKDHMLQYKNIAQVNEEALKQM 1045

Query: 817  ECAHETFKKEAEKMKKSLEVELLQLRERVSELENESILKSQEIASAASLKEEAIASSLAD 876
            ECAHETFK EA+KMKKSLE +LLQLR +VSELENESILKSQE+A AA+LKEEAIASSL +
Sbjct: 1046 ECAHETFKIEADKMKKSLEADLLQLRGKVSELENESILKSQELACAANLKEEAIASSLGE 1105

Query: 877  VRNLKEENAAKTSKIQEMEIKISYLNEDLEREQQRWRTAQANYERQV-----DGKDLINH 936
            +RNL EENAAK+SKIQEMEI+ISYL EDLERE Q+WR AQANYERQV       ++L   
Sbjct: 1106 IRNLSEENAAKSSKIQEMEIQISYLKEDLERELQKWRAAQANYERQVILQSETIQELTKT 1128

Query: 937  PSCLCNLAENGSKKRYFTDHLK 954
               L ++ E  ++ R   D  K
Sbjct: 1166 SQALASVQEEAAELRKLADAYK 1128

BLAST of Spg021421 vs. TAIR 10
Match: AT1G79280.1 (nuclear pore anchor )

HSP 1 Score: 634.8 bits (1636), Expect = 1.1e-181
Identity = 383/727 (52.68%), Postives = 505/727 (69.46%), Query Frame = 0

Query: 231  EENTAEHERMIESYSLLNQKLQNSISEQAILEKTVQELKADLKRHERDYTLIQRENIDLS 290
            +E   E+ER++E+Y L+NQKLQ+S+SEQ+ +EK + ELKADL+R ER+ TL+Q++  DL 
Sbjct: 456  QEERGEYERVVEAYCLVNQKLQDSVSEQSNMEKFIMELKADLRRRERENTLLQKDISDLQ 515

Query: 291  RQVTILLKECRDVQLRCGYTGHDVLENVSNTTSFEINVGSDADKVISEYLLTFKDINGLV 350
            +QVTILLKECRDVQLRCG    D  ++    +  E+ + S+ADK+ISE+LL FKDINGLV
Sbjct: 516  KQVTILLKECRDVQLRCGAARDDDEDDYPLLSDVEMEMESEADKIISEHLLKFKDINGLV 575

Query: 351  EQNVQLRSLVRKLSVQLQDTELDFKEKLEVELKRQTQEAASRVEAVLQKVEEQGQMIESL 410
            EQNV+LR+LVR LS Q++  E + KE  EV+LK +T EA+++V  VL++ EEQGQMIESL
Sbjct: 576  EQNVKLRNLVRSLSEQIESRETELKETFEVDLKNKTDEASAKVATVLKRAEEQGQMIESL 635

Query: 411  HASVAMYKRLYEEEHKRNLPHPLSAGV--ALGFGRKEQEFVSEDSQEATKAAQEQAAKRI 470
            H SVAMYKRLYEEE K +     S+ +  A+  GRK    + EDS+EATK AQE+A +RI
Sbjct: 636  HTSVAMYKRLYEEEQKLHSSDSRSSDLSPAVVPGRKNFLHLLEDSEEATKRAQEKAFERI 695

Query: 471  RYLEEELEKSRSEINLVRAERNKFELESGFAKEKLDSFMKEFEQQRVEMNGVLARNVEFS 530
            R LEE+  K+RSE+  +R+ER+K  +E+ FA+EKL+  MKE E++R EMN VLARN+EFS
Sbjct: 696  RILEEDFAKARSEVIAIRSERDKLAMEANFAREKLEGIMKESERKREEMNSVLARNIEFS 755

Query: 531  QLIVDYQRKLREVSESLHSADEHSRKLTIEVSILKSEKELLSNAEKRAQDEIQNLSERLF 590
            QLI+D+QRKLRE SESLH+A+E SRKL++EVS+LK EKELLSNAEKRA DE+  LS+R++
Sbjct: 756  QLIIDHQRKLRESSESLHAAEEISRKLSMEVSVLKQEKELLSNAEKRASDEVSALSQRVY 815

Query: 591  RVQASLDTIRSVEEVHEEARVVERRKLEEHAKQLEGASAFSSLRKVKIPKKVSRSGVFFV 650
            R+QA+LDT++S EEV EE R  ERRK EEH KQL+                         
Sbjct: 816  RLQATLDTVQSTEEVREETRAAERRKQEEHIKQLQ------------------------- 875

Query: 651  GVKRDILGIAHISYVSYDILYHFHVYDKLYMKREWAEAKKELQEERDNVRTLTLDREKTL 710
                                            REWAEAKKELQEER N R  T DR +TL
Sbjct: 876  --------------------------------REWAEAKKELQEERSNARDFTSDRNQTL 935

Query: 711  KNAMSHVEEMGKELANALHATAAAEARAAVAEAKLSDLAKKISSSESQVVELDDSSGLKS 770
             NA+  VEEMGKELANAL A + AE+RA+VAEA+LSDL KKI SS+ + +++ DS G+ S
Sbjct: 936  NNAVMQVEEMGKELANALKAVSVAESRASVAEARLSDLEKKIRSSDPKTLDM-DSGGIVS 995

Query: 771  QPSDQVGTVLRSAESEIQKFKEEAQACKDHMLQYKSIAQVNEEAVRQMECAHETFKKEAE 830
                ++   LR+A+ EI+K + E ++ K HMLQYKSIAQVNE A++QME AHE F+ EAE
Sbjct: 996  LSDKEMSIELRTAKEEIEKLRGEVESSKSHMLQYKSIAQVNETALKQMESAHENFRLEAE 1055

Query: 831  KMKKSLEVELLQLRERVSELENESILKSQEIASAASLKEEAIASSLADVRNLKEENAAKT 890
            K ++SLE EL+ LRERVSELEN+ I KS+++A+AA+ KE+A+ S+ A++ +L+EEN  K 
Sbjct: 1056 KRQRSLEAELVSLRERVSELENDCIQKSEQLATAAAGKEDALLSASAEIASLREENLVKK 1115

Query: 891  SKIQEMEIKISYLNEDLEREQQRWRTAQANYERQV-----DGKDLINHPSCLCNLAENGS 950
            S+I+ M I++S L  DLE E ++WR AQ NYERQV       ++L      L  L E  S
Sbjct: 1116 SQIEAMNIQMSTLKNDLETEHEKWRVAQRNYERQVILLSETIQELTKTSQALAALQEEAS 1124

BLAST of Spg021421 vs. TAIR 10
Match: AT1G79280.2 (nuclear pore anchor )

HSP 1 Score: 624.4 bits (1609), Expect = 1.5e-178
Identity = 385/749 (51.40%), Postives = 508/749 (67.82%), Query Frame = 0

Query: 231  EENTAEHERMIESYSLLNQKLQNSISEQAILEKTVQELKADLKRHERDYTLIQRENIDLS 290
            +E   E+ER++E+Y L+NQKLQ+S+SEQ+ +EK + ELKADL+R ER+ TL+Q++  DL 
Sbjct: 456  QEERGEYERVVEAYCLVNQKLQDSVSEQSNMEKFIMELKADLRRRERENTLLQKDISDLQ 515

Query: 291  RQVTILLKECRDVQLRCGYTGHDVLENVSNTTSFEINVGSDADKVISEYLLTFKDINGLV 350
            +QVTILLKECRDVQLRCG    D  ++    +  E+ + S+ADK+ISE+LL FKDINGLV
Sbjct: 516  KQVTILLKECRDVQLRCGAARDDDEDDYPLLSDVEMEMESEADKIISEHLLKFKDINGLV 575

Query: 351  EQNVQLRSLVRKLSVQLQDTELDFKEKLEVELKRQTQEAASRVEAVLQKVEEQGQMIESL 410
            EQNV+LR+LVR LS Q++  E + KE  EV+LK +T EA+++V  VL++ EEQGQMIESL
Sbjct: 576  EQNVKLRNLVRSLSEQIESRETELKETFEVDLKNKTDEASAKVATVLKRAEEQGQMIESL 635

Query: 411  HASVAMYKRLYEEEHKRNLPHPLSAGV--ALGFGRKEQEFVSEDSQEATKAAQEQAAKRI 470
            H SVAMYKRLYEEE K +     S+ +  A+  GRK    + EDS+EATK AQE+A +RI
Sbjct: 636  HTSVAMYKRLYEEEQKLHSSDSRSSDLSPAVVPGRKNFLHLLEDSEEATKRAQEKAFERI 695

Query: 471  RYLEEELEKSRSEINLVRAERNKFELESGFAKEKLDSFMKEFEQQRVEMNGVLARNVEFS 530
            R LEE+  K+RSE+  +R+ER+K  +E+ FA+EKL+  MKE E++R EMN VLARN+EFS
Sbjct: 696  RILEEDFAKARSEVIAIRSERDKLAMEANFAREKLEGIMKESERKREEMNSVLARNIEFS 755

Query: 531  QLIVDYQRKLREVSESLHSADEHSRKLTIEVSILKSEKELLSNAEKRAQDEIQNLSERLF 590
            QLI+D+QRKLRE SESLH+A+E SRKL++EVS+LK EKELLSNAEKRA DE+  LS+R++
Sbjct: 756  QLIIDHQRKLRESSESLHAAEEISRKLSMEVSVLKQEKELLSNAEKRASDEVSALSQRVY 815

Query: 591  RVQASLDTIRSVEEVHEEARVVERRKLEEHAKQLEGASAFSSLRKVKIPKKVSRSGVFFV 650
            R+QA+LDT++S EEV EE R  ERRK EEH KQL+                         
Sbjct: 816  RLQATLDTVQSTEEVREETRAAERRKQEEHIKQLQ------------------------- 875

Query: 651  GVKRDILGIAHISYVSYDILYHFHVYDKLYMKREWAEAKKELQEERDNVRTLTLDREKTL 710
                                            REWAEAKKELQEER N R  T DR +TL
Sbjct: 876  --------------------------------REWAEAKKELQEERSNARDFTSDRNQTL 935

Query: 711  KNAMSHVEEMGKELANALHATAAAEARAAVAEAKLSDLAKKISSSESQVVELDDSSGL-- 770
             NA+  VEEMGKELANAL A + AE+RA+VAEA+LSDL KKI SS+ + +++ DS G+  
Sbjct: 936  NNAVMQVEEMGKELANALKAVSVAESRASVAEARLSDLEKKIRSSDPKTLDM-DSGGIVS 995

Query: 771  ----------------KSQPSDQVGTV----LRSAESEIQKFKEEAQACKDHMLQYKSIA 830
                            K Q   ++G+     LR+A+ EI+K + E ++ K HMLQYKSIA
Sbjct: 996  LSDKEAALVIILDVVHKIQAGFRIGSAMSIELRTAKEEIEKLRGEVESSKSHMLQYKSIA 1055

Query: 831  QVNEEAVRQMECAHETFKKEAEKMKKSLEVELLQLRERVSELENESILKSQEIASAASLK 890
            QVNE A++QME AHE F+ EAEK ++SLE EL+ LRERVSELEN+ I KS+++A+AA+ K
Sbjct: 1056 QVNETALKQMESAHENFRLEAEKRQRSLEAELVSLRERVSELENDCIQKSEQLATAAAGK 1115

Query: 891  EEAIASSLADVRNLKEENAAKTSKIQEMEIKISYLNEDLEREQQRWRTAQANYERQV--- 950
            E+A+ S+ A++ +L+EEN  K S+I+ M I++S L  DLE E ++WR AQ NYERQV   
Sbjct: 1116 EDALLSASAEIASLREENLVKKSQIEAMNIQMSTLKNDLETEHEKWRVAQRNYERQVILL 1146

BLAST of Spg021421 vs. TAIR 10
Match: AT1G79280.3 (nuclear pore anchor )

HSP 1 Score: 619.4 bits (1596), Expect = 4.9e-177
Identity = 379/728 (52.06%), Postives = 501/728 (68.82%), Query Frame = 0

Query: 231  EENTAEHERMIESYSLLNQKLQNSISEQAILEKTVQELKADLKRHERDYTLIQRENIDLS 290
            +E   E+ER++E+Y L+NQKLQ+S+SEQ+ +EK + ELKADL+R ER+ TL+Q++  DL 
Sbjct: 456  QEERGEYERVVEAYCLVNQKLQDSVSEQSNMEKFIMELKADLRRRERENTLLQKDISDLQ 515

Query: 291  RQVTILLKECRDVQLRCGYTGHDVLENVSNTTSFEINVGSDADKVISEYLLTFKDINGLV 350
            +QVTILLKECRDVQLRCG    D  ++    +  E+ + S+ADK+ISE+LL FKDINGLV
Sbjct: 516  KQVTILLKECRDVQLRCGAARDDDEDDYPLLSDVEMEMESEADKIISEHLLKFKDINGLV 575

Query: 351  EQNVQLRSLVRKLSVQLQDTELDFKEKLEVELKRQTQEAASRVEAVLQKVEEQGQMIESL 410
            EQNV+LR+LVR LS Q++  E + KE  EV+LK +T EA+++V  VL++ EEQGQMIESL
Sbjct: 576  EQNVKLRNLVRSLSEQIESRETELKETFEVDLKNKTDEASAKVATVLKRAEEQGQMIESL 635

Query: 411  HASVAMYKRLYEEEHKRNLPHPLSAGV--ALGFGRKEQEFVSEDSQEATKAAQEQAAKRI 470
            H SVAMYKRLYEEE K +     S+ +  A+  GRK    + EDS+EATK AQE+A +RI
Sbjct: 636  HTSVAMYKRLYEEEQKLHSSDSRSSDLSPAVVPGRKNFLHLLEDSEEATKRAQEKAFERI 695

Query: 471  RYLEEELEKSRSEINLVRAERNKFELESGFAKEKLDSFMKEFEQQRVEMNGVLARNVEFS 530
            R LEE+  K+RSE+  +R+ER+K  +E+ FA+EKL+  MKE E++R EMN VLARN+EFS
Sbjct: 696  RILEEDFAKARSEVIAIRSERDKLAMEANFAREKLEGIMKESERKREEMNSVLARNIEFS 755

Query: 531  QLIVDYQRKLREVSESLHSADEHSRKLTIEVSILKS-EKELLSNAEKRAQDEIQNLSERL 590
            QLI+D+QRKLRE SESLH+A+E SRKL++EV      +KELLSNAEKRA DE+  LS+R+
Sbjct: 756  QLIIDHQRKLRESSESLHAAEEISRKLSMEVVYHHPLKKELLSNAEKRASDEVSALSQRV 815

Query: 591  FRVQASLDTIRSVEEVHEEARVVERRKLEEHAKQLEGASAFSSLRKVKIPKKVSRSGVFF 650
            +R+QA+LDT++S EEV EE R  ERRK EEH KQL+                        
Sbjct: 816  YRLQATLDTVQSTEEVREETRAAERRKQEEHIKQLQ------------------------ 875

Query: 651  VGVKRDILGIAHISYVSYDILYHFHVYDKLYMKREWAEAKKELQEERDNVRTLTLDREKT 710
                                             REWAEAKKELQEER N R  T DR +T
Sbjct: 876  ---------------------------------REWAEAKKELQEERSNARDFTSDRNQT 935

Query: 711  LKNAMSHVEEMGKELANALHATAAAEARAAVAEAKLSDLAKKISSSESQVVELDDSSGLK 770
            L NA+  VEEMGKELANAL A + AE+RA+VAEA+LSDL KKI SS+ + +++ DS G+ 
Sbjct: 936  LNNAVMQVEEMGKELANALKAVSVAESRASVAEARLSDLEKKIRSSDPKTLDM-DSGGIV 995

Query: 771  SQPSDQVGTVLRSAESEIQKFKEEAQACKDHMLQYKSIAQVNEEAVRQMECAHETFKKEA 830
            S    ++   LR+A+ EI+K + E ++ K HMLQYKSIAQVNE A++QME AHE F+ EA
Sbjct: 996  SLSDKEMSIELRTAKEEIEKLRGEVESSKSHMLQYKSIAQVNETALKQMESAHENFRLEA 1055

Query: 831  EKMKKSLEVELLQLRERVSELENESILKSQEIASAASLKEEAIASSLADVRNLKEENAAK 890
            EK ++SLE EL+ LRERVSELEN+ I KS+++A+AA+ KE+A+ S+ A++ +L+EEN  K
Sbjct: 1056 EKRQRSLEAELVSLRERVSELENDCIQKSEQLATAAAGKEDALLSASAEIASLREENLVK 1115

Query: 891  TSKIQEMEIKISYLNEDLEREQQRWRTAQANYERQV-----DGKDLINHPSCLCNLAENG 950
             S+I+ M I++S L  DLE E ++WR AQ NYERQV       ++L      L  L E  
Sbjct: 1116 KSQIEAMNIQMSTLKNDLETEHEKWRVAQRNYERQVILLSETIQELTKTSQALAALQEEA 1125