Homology
BLAST of Spg021407 vs. NCBI nr
Match:
XP_022147909.1 (ent-kaur-16-ene synthase, chloroplastic isoform X2 [Momordica charantia])
HSP 1 Score: 917.9 bits (2371), Expect = 5.1e-263
Identity = 454/744 (61.02%), Postives = 558/744 (75.00%), Query Frame = 0
Query: 3 CFEGTKERIKKMFEKVELSVSAYDTAWMAMVPTPNCLKTPLFPECLNWLLDNQLPDGSWG 62
CFE TKERIKKMF KVELSVSAYDTAW+AMVP+PN L PLFPEC+NW+LDNQ PDGSWG
Sbjct: 85 CFEETKERIKKMFNKVELSVSAYDTAWVAMVPSPNSLNDPLFPECINWVLDNQHPDGSWG 144
Query: 63 LLHNYPILTKATLLSTFASILALKRWDVGQEHMIKGLQFIESNLDSATDEKQHSPIGFDI 122
++HN+ +L KA+LLST A L LKRW++G EHM K L FI+SN+ SATDEKQ+SP+GFDI
Sbjct: 145 IIHNHHLLMKASLLSTLACALTLKRWNIGHEHMSKALNFIKSNIVSATDEKQYSPVGFDI 204
Query: 123 IFSGMIEYAKTLDLSLPLPQSNIDALIRRRELELSRSNSEGKKAYLAYVSEGLGKSQDWG 182
IF GMIEYAK LDL+LPL +N+DAL++++EL+L RSNSEG KAYLAYVSEG+G+ +DW
Sbjct: 205 IFPGMIEYAKDLDLNLPLAPANVDALLQKKELDLRRSNSEGGKAYLAYVSEGIGRLRDWE 264
Query: 183 MVMNFQRKNGSLFNSPSTTAAAFMNLNNDSCLDYLHSLVQKFGNAVPTIYPLDIYAQLCM 242
MVM +QRKNGSLFNSPSTTA AFM+ N+D C +YLHSL+QKF N+VPTIYPLDIYA+LCM
Sbjct: 265 MVMQYQRKNGSLFNSPSTTAVAFMHGNDDGCFNYLHSLLQKFDNSVPTIYPLDIYARLCM 324
Query: 243 VDNLQTLGIDRHFKEEIGSLLDKTYRHWLKGDENILLDASTCAMAFRMLH---------Q 302
VDNLQ LGIDRHFKEEI S+LD+TYR W++G+E+I LDASTCAMAFRML Q
Sbjct: 325 VDNLQRLGIDRHFKEEIRSVLDETYRCWMQGEEDIFLDASTCAMAFRMLRLGGYDVSSDQ 384
Query: 303 LAQFSEDRFSDCLGGYLKDIGSILELFKASQITICPEESVLDNINTWTN----------- 362
L QFSED FS+CLGGYLKD GS LELFKASQI I P+ES+L+NIN+WTN
Sbjct: 385 LTQFSEDLFSNCLGGYLKDFGSSLELFKASQIIIYPDESILENINSWTNSFLSHGLSSGS 444
Query: 363 -------------------------LNRIFRKLS-----------TLVNFSCLNLGHQDF 422
L R+ K + + ++CLN GHQDF
Sbjct: 445 VHSDRPDRLLKQEAINALEFTYSNTLERLLNKRAMENYNIDIVRISKTTYTCLNFGHQDF 504
Query: 423 LRLAMEEFYGCQCIQRQEKKDLERWVIENKLEKL-LPRHRVVYCYFTGAALITSPELTEA 482
L LA+E+F Q I +E K+LERWV+EN+L+KL R ++ YCYF+ AA +TSPEL +A
Sbjct: 505 LELAVEDFNILQSIHHKELKELERWVVENRLDKLKFARQKLAYCYFSAAATLTSPELRDA 564
Query: 483 RMSFGKSGVLTTVLDAFFDDWGSQDERTNLVQAAERWDVSNEMEFCSEEVEIIFKALRRA 542
R+S+ K+GVLTTV+D FFD GS+ E NL+Q E+WDV+ E ++CS+EVEIIF AL
Sbjct: 565 RLSWAKNGVLTTVVDDFFDVGGSEGELINLIQLVEKWDVNGEADYCSKEVEIIFLALHGT 624
Query: 543 VDEISNAALTWQGRSVRSHVIDTWVELIKSMAMEADWSSNKTVPSMDEYIETSAVSFALG 602
V EI AL WQGRSV +VID W+ L+KSM EA+W NK P MDEY+E +SFALG
Sbjct: 625 VCEIEKRALPWQGRSVMRNVIDIWLALLKSMRKEAEWLKNKIAPPMDEYMENGYISFALG 684
Query: 603 PIVLPALYFIGPKISEEMVGSFEYHNLFMLMSIAGRLLNDIKTIEKEAGEGKHNAFSLRM 662
PIVLPAL+F+GPK+ EE++GS EYH LF LMS GRLLND ++ E+E+ EGK NA SL M
Sbjct: 685 PIVLPALFFVGPKLPEEVIGSCEYHKLFKLMSTCGRLLNDTQSFERESSEGKLNAMSLCM 744
Query: 663 LDGGVHITKEEAIRAIQVDIDRATRKLLRLVLQVEDSTIPRVCKDLFWRVNCAVHVFYRK 690
++ G TKEEA AI+ +++R R+LLRLVLQ ++ST+PR CKDLFW+++C VH+FYRK
Sbjct: 745 INAGGDFTKEEASEAIKGNVERTRRELLRLVLQEKNSTVPRACKDLFWKMSCVVHLFYRK 804
BLAST of Spg021407 vs. NCBI nr
Match:
XP_022147908.1 (ent-kaur-16-ene synthase, chloroplastic isoform X1 [Momordica charantia])
HSP 1 Score: 912.9 bits (2358), Expect = 1.7e-261
Identity = 454/746 (60.86%), Postives = 558/746 (74.80%), Query Frame = 0
Query: 3 CFEGTKERIKKMFEKVELSVSAYDTAWMAMVPTPNCLKTPLFPECLNWLLDNQLPDGSWG 62
CFE TKERIKKMF KVELSVSAYDTAW+AMVP+PN L PLFPEC+NW+LDNQ PDGSWG
Sbjct: 85 CFEETKERIKKMFNKVELSVSAYDTAWVAMVPSPNSLNDPLFPECINWVLDNQHPDGSWG 144
Query: 63 LLHNYPILTKATLLSTFASILALKRWDVGQEHMIKGLQFIESNLDSATDEKQHSPIGFDI 122
++HN+ +L KA+LLST A L LKRW++G EHM K L FI+SN+ SATDEKQ+SP+GFDI
Sbjct: 145 IIHNHHLLMKASLLSTLACALTLKRWNIGHEHMSKALNFIKSNIVSATDEKQYSPVGFDI 204
Query: 123 IFSGMIEYAKTLDLSLPLPQSNIDALIRRRELEL--SRSNSEGKKAYLAYVSEGLGKSQD 182
IF GMIEYAK LDL+LPL +N+DAL++++EL+L RSNSEG KAYLAYVSEG+G+ +D
Sbjct: 205 IFPGMIEYAKDLDLNLPLAPANVDALLQKKELDLRSCRSNSEGGKAYLAYVSEGIGRLRD 264
Query: 183 WGMVMNFQRKNGSLFNSPSTTAAAFMNLNNDSCLDYLHSLVQKFGNAVPTIYPLDIYAQL 242
W MVM +QRKNGSLFNSPSTTA AFM+ N+D C +YLHSL+QKF N+VPTIYPLDIYA+L
Sbjct: 265 WEMVMQYQRKNGSLFNSPSTTAVAFMHGNDDGCFNYLHSLLQKFDNSVPTIYPLDIYARL 324
Query: 243 CMVDNLQTLGIDRHFKEEIGSLLDKTYRHWLKGDENILLDASTCAMAFRMLH-------- 302
CMVDNLQ LGIDRHFKEEI S+LD+TYR W++G+E+I LDASTCAMAFRML
Sbjct: 325 CMVDNLQRLGIDRHFKEEIRSVLDETYRCWMQGEEDIFLDASTCAMAFRMLRLGGYDVSS 384
Query: 303 -QLAQFSEDRFSDCLGGYLKDIGSILELFKASQITICPEESVLDNINTWTN--------- 362
QL QFSED FS+CLGGYLKD GS LELFKASQI I P+ES+L+NIN+WTN
Sbjct: 385 DQLTQFSEDLFSNCLGGYLKDFGSSLELFKASQIIIYPDESILENINSWTNSFLSHGLSS 444
Query: 363 ---------------------------LNRIFRKLS-----------TLVNFSCLNLGHQ 422
L R+ K + + ++CLN GHQ
Sbjct: 445 GSVHSDRPDRLLKQEAINALEFTYSNTLERLLNKRAMENYNIDIVRISKTTYTCLNFGHQ 504
Query: 423 DFLRLAMEEFYGCQCIQRQEKKDLERWVIENKLEKL-LPRHRVVYCYFTGAALITSPELT 482
DFL LA+E+F Q I +E K+LERWV+EN+L+KL R ++ YCYF+ AA +TSPEL
Sbjct: 505 DFLELAVEDFNILQSIHHKELKELERWVVENRLDKLKFARQKLAYCYFSAAATLTSPELR 564
Query: 483 EARMSFGKSGVLTTVLDAFFDDWGSQDERTNLVQAAERWDVSNEMEFCSEEVEIIFKALR 542
+AR+S+ K+GVLTTV+D FFD GS+ E NL+Q E+WDV+ E ++CS+EVEIIF AL
Sbjct: 565 DARLSWAKNGVLTTVVDDFFDVGGSEGELINLIQLVEKWDVNGEADYCSKEVEIIFLALH 624
Query: 543 RAVDEISNAALTWQGRSVRSHVIDTWVELIKSMAMEADWSSNKTVPSMDEYIETSAVSFA 602
V EI AL WQGRSV +VID W+ L+KSM EA+W NK P MDEY+E +SFA
Sbjct: 625 GTVCEIEKRALPWQGRSVMRNVIDIWLALLKSMRKEAEWLKNKIAPPMDEYMENGYISFA 684
Query: 603 LGPIVLPALYFIGPKISEEMVGSFEYHNLFMLMSIAGRLLNDIKTIEKEAGEGKHNAFSL 662
LGPIVLPAL+F+GPK+ EE++GS EYH LF LMS GRLLND ++ E+E+ EGK NA SL
Sbjct: 685 LGPIVLPALFFVGPKLPEEVIGSCEYHKLFKLMSTCGRLLNDTQSFERESSEGKLNAMSL 744
Query: 663 RMLDGGVHITKEEAIRAIQVDIDRATRKLLRLVLQVEDSTIPRVCKDLFWRVNCAVHVFY 690
M++ G TKEEA AI+ +++R R+LLRLVLQ ++ST+PR CKDLFW+++C VH+FY
Sbjct: 745 CMINAGGDFTKEEASEAIKGNVERTRRELLRLVLQEKNSTVPRACKDLFWKMSCVVHLFY 804
BLAST of Spg021407 vs. NCBI nr
Match:
XP_022147910.1 (ent-kaur-16-ene synthase, chloroplastic isoform X3 [Momordica charantia])
HSP 1 Score: 912.9 bits (2358), Expect = 1.7e-261
Identity = 454/746 (60.86%), Postives = 558/746 (74.80%), Query Frame = 0
Query: 3 CFEGTKERIKKMFEKVELSVSAYDTAWMAMVPTPNCLKTPLFPECLNWLLDNQLPDGSWG 62
CFE TKERIKKMF KVELSVSAYDTAW+AMVP+PN L PLFPEC+NW+LDNQ PDGSWG
Sbjct: 72 CFEETKERIKKMFNKVELSVSAYDTAWVAMVPSPNSLNDPLFPECINWVLDNQHPDGSWG 131
Query: 63 LLHNYPILTKATLLSTFASILALKRWDVGQEHMIKGLQFIESNLDSATDEKQHSPIGFDI 122
++HN+ +L KA+LLST A L LKRW++G EHM K L FI+SN+ SATDEKQ+SP+GFDI
Sbjct: 132 IIHNHHLLMKASLLSTLACALTLKRWNIGHEHMSKALNFIKSNIVSATDEKQYSPVGFDI 191
Query: 123 IFSGMIEYAKTLDLSLPLPQSNIDALIRRRELEL--SRSNSEGKKAYLAYVSEGLGKSQD 182
IF GMIEYAK LDL+LPL +N+DAL++++EL+L RSNSEG KAYLAYVSEG+G+ +D
Sbjct: 192 IFPGMIEYAKDLDLNLPLAPANVDALLQKKELDLRSCRSNSEGGKAYLAYVSEGIGRLRD 251
Query: 183 WGMVMNFQRKNGSLFNSPSTTAAAFMNLNNDSCLDYLHSLVQKFGNAVPTIYPLDIYAQL 242
W MVM +QRKNGSLFNSPSTTA AFM+ N+D C +YLHSL+QKF N+VPTIYPLDIYA+L
Sbjct: 252 WEMVMQYQRKNGSLFNSPSTTAVAFMHGNDDGCFNYLHSLLQKFDNSVPTIYPLDIYARL 311
Query: 243 CMVDNLQTLGIDRHFKEEIGSLLDKTYRHWLKGDENILLDASTCAMAFRMLH-------- 302
CMVDNLQ LGIDRHFKEEI S+LD+TYR W++G+E+I LDASTCAMAFRML
Sbjct: 312 CMVDNLQRLGIDRHFKEEIRSVLDETYRCWMQGEEDIFLDASTCAMAFRMLRLGGYDVSS 371
Query: 303 -QLAQFSEDRFSDCLGGYLKDIGSILELFKASQITICPEESVLDNINTWTN--------- 362
QL QFSED FS+CLGGYLKD GS LELFKASQI I P+ES+L+NIN+WTN
Sbjct: 372 DQLTQFSEDLFSNCLGGYLKDFGSSLELFKASQIIIYPDESILENINSWTNSFLSHGLSS 431
Query: 363 ---------------------------LNRIFRKLS-----------TLVNFSCLNLGHQ 422
L R+ K + + ++CLN GHQ
Sbjct: 432 GSVHSDRPDRLLKQEAINALEFTYSNTLERLLNKRAMENYNIDIVRISKTTYTCLNFGHQ 491
Query: 423 DFLRLAMEEFYGCQCIQRQEKKDLERWVIENKLEKL-LPRHRVVYCYFTGAALITSPELT 482
DFL LA+E+F Q I +E K+LERWV+EN+L+KL R ++ YCYF+ AA +TSPEL
Sbjct: 492 DFLELAVEDFNILQSIHHKELKELERWVVENRLDKLKFARQKLAYCYFSAAATLTSPELR 551
Query: 483 EARMSFGKSGVLTTVLDAFFDDWGSQDERTNLVQAAERWDVSNEMEFCSEEVEIIFKALR 542
+AR+S+ K+GVLTTV+D FFD GS+ E NL+Q E+WDV+ E ++CS+EVEIIF AL
Sbjct: 552 DARLSWAKNGVLTTVVDDFFDVGGSEGELINLIQLVEKWDVNGEADYCSKEVEIIFLALH 611
Query: 543 RAVDEISNAALTWQGRSVRSHVIDTWVELIKSMAMEADWSSNKTVPSMDEYIETSAVSFA 602
V EI AL WQGRSV +VID W+ L+KSM EA+W NK P MDEY+E +SFA
Sbjct: 612 GTVCEIEKRALPWQGRSVMRNVIDIWLALLKSMRKEAEWLKNKIAPPMDEYMENGYISFA 671
Query: 603 LGPIVLPALYFIGPKISEEMVGSFEYHNLFMLMSIAGRLLNDIKTIEKEAGEGKHNAFSL 662
LGPIVLPAL+F+GPK+ EE++GS EYH LF LMS GRLLND ++ E+E+ EGK NA SL
Sbjct: 672 LGPIVLPALFFVGPKLPEEVIGSCEYHKLFKLMSTCGRLLNDTQSFERESSEGKLNAMSL 731
Query: 663 RMLDGGVHITKEEAIRAIQVDIDRATRKLLRLVLQVEDSTIPRVCKDLFWRVNCAVHVFY 690
M++ G TKEEA AI+ +++R R+LLRLVLQ ++ST+PR CKDLFW+++C VH+FY
Sbjct: 732 CMINAGGDFTKEEASEAIKGNVERTRRELLRLVLQEKNSTVPRACKDLFWKMSCVVHLFY 791
BLAST of Spg021407 vs. NCBI nr
Match:
XP_016900364.1 (PREDICTED: ent-kaur-16-ene synthase, chloroplastic isoform X2 [Cucumis melo])
HSP 1 Score: 884.0 bits (2283), Expect = 8.2e-253
Identity = 440/743 (59.22%), Postives = 553/743 (74.43%), Query Frame = 0
Query: 4 FEGTKERIKKMFEKVELSVSAYDTAWMAMVPTPNCLKTPLFPECLNWLLDNQLPDGSWGL 63
FE TKERI+K+F+ VELSVSAYDTAW+AMVP+PN L PLFPEC+NW+LD+Q PDGSWG+
Sbjct: 37 FEDTKERIQKLFKNVELSVSAYDTAWVAMVPSPNSLNKPLFPECINWVLDHQNPDGSWGI 96
Query: 64 LHNYPILTKATLLSTFASILALKRWDVGQEHMIKGLQFIESNLDSATDEKQHSPIGFDII 123
LH++ ++ KATLLST A +L LKRW++G +HM K L FI+SN+ SATDE Q SP+GFDII
Sbjct: 97 LHDHQLVMKATLLSTLACVLTLKRWNIGHDHMSKALSFIKSNIASATDENQRSPVGFDII 156
Query: 124 FSGMIEYAKTLDLSLPLPQSNIDALIRRRELELSRSNSEGKKAYLAYVSEGLGKSQDWGM 183
F GMIEYAK L+L+LPL N+DAL++++ELEL RSNSEG KAYLAYVSEG+GK QDW M
Sbjct: 157 FPGMIEYAKDLNLNLPLASMNVDALVQKKELEL-RSNSEGGKAYLAYVSEGIGKLQDWDM 216
Query: 184 VMNFQRKNGSLFNSPSTTAAAFMNLNNDSCLDYLHSLVQKFGNAVPTIYPLDIYAQLCMV 243
VM +QRKNGSLF+SPSTTA AFM+ N+D C +YL S++QKF ++VP IYPLDIYA+L MV
Sbjct: 217 VMQYQRKNGSLFSSPSTTAVAFMHRNDDGCFNYLRSILQKFHSSVPAIYPLDIYARLHMV 276
Query: 244 DNLQTLGIDRHFKEEIGSLLDKTYRHWLKGDENILLDASTCAMAFRMLH---------QL 303
D+LQ LGIDRHFK+EI S+LD+TYR W++G+ENI LDASTCAMAFRML QL
Sbjct: 277 DSLQKLGIDRHFKDEIRSVLDETYRCWMQGEENIFLDASTCAMAFRMLRVEGYDVSSDQL 336
Query: 304 AQFSEDRFSDCLGGYLKDIGSILELFKASQITICPEESVLDNINTWTN------------ 363
QFSED F +CLGG+LKD G+ LELFKASQI I P+E +L+NIN+WT+
Sbjct: 337 TQFSEDLFPNCLGGHLKDFGASLELFKASQIIIYPDEFILENINSWTSRFLNHGLSGGSV 396
Query: 364 ------------------------LNRIFRKLS-----------TLVNFSCLNLGHQDFL 423
L R+ K + + ++CLN GHQDFL
Sbjct: 397 HSDRTERLVKQEAVNAFEFPYNSTLERLSNKRALESYSGDIVRISKTTYACLNFGHQDFL 456
Query: 424 RLAMEEFYGCQCIQRQEKKDLERWVIENKLEKL-LPRHRVVYCYFTGAALITSPELTEAR 483
LA+E+F QCI R+E K+LE+WVIENKL+KL R ++ YCYF+ AA +TSPEL +AR
Sbjct: 457 ELAVEDFNTLQCIHRKELKELEKWVIENKLDKLKFARQKLAYCYFSAAATLTSPELCDAR 516
Query: 484 MSFGKSGVLTTVLDAFFDDWGSQDERTNLVQAAERWDVSNEMEFCSEEVEIIFKALRRAV 543
+S+ K+GVLTTV+D FFD GS++E NL+Q E+WD S E +CS+EVEIIF AL +
Sbjct: 517 LSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVEKWDASGEAGYCSKEVEIIFLALHSTI 576
Query: 544 DEISNAALTWQGRSVRSHVIDTWVELIKSMAMEADWSSNKTVPSMDEYIETSAVSFALGP 603
EI AL WQGRSV +VID W+ L++SM EA+W NK VPS+DEY+E VSFALGP
Sbjct: 577 CEIGEKALPWQGRSVTRNVIDIWLALLESMRKEAEWLKNKVVPSLDEYMENGYVSFALGP 636
Query: 604 IVLPALYFIGPKISEEMVGSFEYHNLFMLMSIAGRLLNDIKTIEKEAGEGKHNAFSLRML 663
IVLP LYF+GPK+ EE++G+ EY LF LMS +GRLLND +T ++E+ EGK NA SL ++
Sbjct: 637 IVLPTLYFVGPKLPEEIIGNCEYQKLFKLMSTSGRLLNDTRTFDRESSEGKLNALSLYLI 696
Query: 664 DGGVHITKEEAIRAIQVDIDRATRKLLRLVLQVEDSTIPRVCKDLFWRVNCAVHVFYRKD 690
G ++KEEA A++ D+DR R+LLRLVLQ E+STIPR CKDLFW+++C VH+FYRKD
Sbjct: 697 SAGGKLSKEEATEAMKGDVDRTRRELLRLVLQ-ENSTIPRACKDLFWKMSCVVHLFYRKD 756
BLAST of Spg021407 vs. NCBI nr
Match:
KAA0062808.1 (ent-kaur-16-ene synthase [Cucumis melo var. makuwa])
HSP 1 Score: 880.9 bits (2275), Expect = 7.0e-252
Identity = 439/745 (58.93%), Postives = 552/745 (74.09%), Query Frame = 0
Query: 4 FEGTKERIKKMFEKVELSVSAYDTAWMAMVPTPNCLKTPLFPECLNWLLDNQLPDGSWGL 63
FE TKERI+K+F+ VELSVSAYDTAW+AMVP+PN L PLFPEC+NW+LD+Q PDGSWG+
Sbjct: 111 FEDTKERIQKLFKNVELSVSAYDTAWVAMVPSPNSLNRPLFPECINWVLDHQNPDGSWGI 170
Query: 64 LHNYPILTKATLLSTFASILALKRWDVGQEHMIKGLQFIESNLDSATDEKQHSPIGFDII 123
LH++ ++ KATLLST A +L LKRW++G +HM K L FI+SN+ SATDE Q SP+GFDII
Sbjct: 171 LHDHQLVMKATLLSTLACVLTLKRWNIGHDHMSKALSFIKSNIASATDENQRSPVGFDII 230
Query: 124 FSGMIEYAKTLDLSLPLPQSNIDALIRRRELELSR--SNSEGKKAYLAYVSEGLGKSQDW 183
F GMIEYAK L+L+LPL N+DAL++++ELEL SNSEG KAYLAYVSEG+GK QDW
Sbjct: 231 FPGMIEYAKDLNLNLPLASMNVDALVQKKELELRSCCSNSEGGKAYLAYVSEGIGKLQDW 290
Query: 184 GMVMNFQRKNGSLFNSPSTTAAAFMNLNNDSCLDYLHSLVQKFGNAVPTIYPLDIYAQLC 243
MVM +QRKNGSLF+SPSTTA AFM+ N+D C +YL S++QKF ++VP IYPLDIYA+L
Sbjct: 291 DMVMQYQRKNGSLFSSPSTTAVAFMHRNDDGCFNYLRSILQKFHSSVPAIYPLDIYARLH 350
Query: 244 MVDNLQTLGIDRHFKEEIGSLLDKTYRHWLKGDENILLDASTCAMAFRMLH--------- 303
MVD+LQ LGIDRHFK+EI S+LD+TYR W++G+ENI LDASTCAMAFRML
Sbjct: 351 MVDSLQKLGIDRHFKDEIRSVLDETYRCWMQGEENIFLDASTCAMAFRMLRVEGYDVSSD 410
Query: 304 QLAQFSEDRFSDCLGGYLKDIGSILELFKASQITICPEESVLDNINTWTN---------- 363
QL QFSED F +CLGG+LKD G+ LELFKASQI I P+E +L+NIN+WT+
Sbjct: 411 QLTQFSEDLFPNCLGGHLKDFGASLELFKASQIIIYPDEFILENINSWTSRFLNHGLSGG 470
Query: 364 --------------------------LNRIFRKLS-----------TLVNFSCLNLGHQD 423
L R+ K + + ++CLN GHQD
Sbjct: 471 SVHFDRTERLVKQEAVNAFEFPYNSTLERLSNKRALESYSGDIVRISKTTYACLNFGHQD 530
Query: 424 FLRLAMEEFYGCQCIQRQEKKDLERWVIENKLEKL-LPRHRVVYCYFTGAALITSPELTE 483
FL LA+E+F QCI R+E K+LE+WVIENKL+KL R ++ YCYF+ AA +TSPEL +
Sbjct: 531 FLELAVEDFNTLQCIHRKELKELEKWVIENKLDKLKFARQKLAYCYFSAAATLTSPELCD 590
Query: 484 ARMSFGKSGVLTTVLDAFFDDWGSQDERTNLVQAAERWDVSNEMEFCSEEVEIIFKALRR 543
AR+S+ K+GVLTTV+D FFD GS++E NL+Q E+WD S E +CS+EVEIIF AL
Sbjct: 591 ARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVEKWDASGEAGYCSKEVEIIFLALHS 650
Query: 544 AVDEISNAALTWQGRSVRSHVIDTWVELIKSMAMEADWSSNKTVPSMDEYIETSAVSFAL 603
+ EI AL WQGRSV +VID W+ L++SM EA+W NK VPS+DEY+E VSFAL
Sbjct: 651 TICEIGEKALPWQGRSVTRNVIDIWLALLESMRKEAEWLKNKVVPSLDEYMENGYVSFAL 710
Query: 604 GPIVLPALYFIGPKISEEMVGSFEYHNLFMLMSIAGRLLNDIKTIEKEAGEGKHNAFSLR 663
GPIVLP LYF+GPK+ EE++G+ EY LF LMS +GRLLND +T ++E+ EGK NA SL
Sbjct: 711 GPIVLPTLYFVGPKLPEEIIGNCEYQKLFKLMSTSGRLLNDTRTFDRESSEGKLNALSLY 770
Query: 664 MLDGGVHITKEEAIRAIQVDIDRATRKLLRLVLQVEDSTIPRVCKDLFWRVNCAVHVFYR 690
++ G ++KEEA A++ D+DR R+LLRLVLQ E+STIPR CKDLFW+++C VH+FYR
Sbjct: 771 LISAGGKLSKEEATEAMKGDVDRTRRELLRLVLQ-ENSTIPRACKDLFWKMSCVVHLFYR 830
BLAST of Spg021407 vs. ExPASy Swiss-Prot
Match:
Q39548 (Ent-kaur-16-ene synthase, chloroplastic OS=Cucurbita maxima OX=3661 PE=1 SV=1)
HSP 1 Score: 854.0 bits (2205), Expect = 1.2e-246
Identity = 433/745 (58.12%), Postives = 545/745 (73.15%), Query Frame = 0
Query: 4 FEGTKERIKKMFEKVELSVSAYDTAWMAMVPTPNCLKTPLFPECLNWLLDNQLPDGSWGL 63
FE TKERIKK+F+KVELSVSAYDTAW+AMVP+PN L PLFPEC+NW+LD+Q DGSWGL
Sbjct: 41 FEETKERIKKLFDKVELSVSAYDTAWVAMVPSPNSLNQPLFPECINWVLDSQHADGSWGL 100
Query: 64 LHNYPILTKATLLSTFASILALKRWDVGQEHMIKGLQFIESNLDSATDEKQHSPIGFDII 123
LHN +L KA LLST A +L LKRW++G +HM K L FI+SN+ SATDE Q SP+GFDII
Sbjct: 101 LHNDQLLMKANLLSTLACVLTLKRWNIGHDHMSKALDFIKSNIASATDENQRSPVGFDII 160
Query: 124 FSGMIEYAKTLDLSLPLPQSNIDALIRRRELEL--SRSNSEGKKAYLAYVSEGLGKSQDW 183
F GMIEYAK L+L+LPL +N+DAL+R++ELEL RSNSEG KAYLAYVSEG+GK QDW
Sbjct: 161 FPGMIEYAKDLNLNLPLAPTNVDALVRKKELELRSCRSNSEGGKAYLAYVSEGIGKLQDW 220
Query: 184 GMVMNFQRKNGSLFNSPSTTAAAFMNLNNDSCLDYLHSLVQKFGNAVPTIYPLDIYAQLC 243
MVM +QRKNGSLFNSPSTTAAAFM+ N+D C DYL SL+QKF +VPTIYPLDIYA+L
Sbjct: 221 DMVMQYQRKNGSLFNSPSTTAAAFMHRNDDGCFDYLRSLLQKFDGSVPTIYPLDIYARLH 280
Query: 244 MVDNLQTLGIDRHFKEEIGSLLDKTYRHWLKGDENILLDASTCAMAFRMLH--------- 303
MVD+LQ GI RHFKEEI S+LD+TYR W++G+ENI LDASTCAMAFRML
Sbjct: 281 MVDSLQKFGIARHFKEEIRSVLDETYRCWMQGEENIFLDASTCAMAFRMLRVEGYDVSSD 340
Query: 304 QLAQFSEDRFSDCLGGYLKDIGSILELFKASQITICPEESVLDNINTWTN---------- 363
QL QFSED F +CLGGYLKD G+ LEL+KASQI P+ESVL+NIN+WT+
Sbjct: 341 QLTQFSEDIFPNCLGGYLKDFGASLELYKASQIITHPDESVLENINSWTSRFLKHGLSSD 400
Query: 364 --------------------------LNRIFRKLS-----------TLVNFSCLNLGHQD 423
L R+ K + + ++CLN GHQD
Sbjct: 401 SVWSDRTDSVVKQEAVNALEFPYNATLERLISKRAMESYSGDIVRISKSPYACLNFGHQD 460
Query: 424 FLRLAMEEFYGCQCIQRQEKKDLERWVIENKLEKL-LPRHRVVYCYFTGAALITSPELTE 483
FL LA+E+F Q I +E ++L+RWV+ENKL++L R + YCYF AA +T PEL +
Sbjct: 461 FLELAVEDFNTLQRIHLKELEELQRWVVENKLDELKFFRLHLGYCYFAAAATLTDPELHD 520
Query: 484 ARMSFGKSGVLTTVLDAFFDDWGSQDERTNLVQAAERWDVSNEMEFCSEEVEIIFKALRR 543
AR+++ ++GVLTTV+D F+D GS++E NL++ E+WD E+ +CS++VEI+F AL
Sbjct: 521 ARIAWAQNGVLTTVVDDFYDGGGSEEELDNLIELVEKWDPDGEVGYCSKDVEIVFLALHS 580
Query: 544 AVDEISNAALTWQGRSVRSHVIDTWVELIKSMAMEADWSSNKTVPSMDEYIETSAVSFAL 603
V EI AL WQGRSV +VID W+ L+K M EA+WS+NK VPSM EY+E + VSFAL
Sbjct: 581 TVCEIGRRALVWQGRSVMRNVIDGWLALLKVMRKEAEWSTNKVVPSMGEYMEQAHVSFAL 640
Query: 604 GPIVLPALYFIGPKISEEMVGSFEYHNLFMLMSIAGRLLNDIKTIEKEAGEGKHNAFSLR 663
GPI+LP L+F+GPK+SEEM+GS EY L+ LMS AGRL NDI++ ++E EGK N SL
Sbjct: 641 GPIILPMLFFVGPKLSEEMIGSCEYQKLYKLMSTAGRLKNDIRSYDRECKEGKLNILSLW 700
Query: 664 MLDGGVHITKEEAIRAIQVDIDRATRKLLRLVLQVEDSTIPRVCKDLFWRVNCAVHVFYR 690
M+DGG ++TKEEAI AI+ D +RA R+LL LVLQ E++TIPR CKDLFW++ V++FY
Sbjct: 701 MIDGGGNVTKEEAIEAIKGDFERAIRELLGLVLQ-ENTTIPRACKDLFWKLMSIVNLFYM 760
BLAST of Spg021407 vs. ExPASy Swiss-Prot
Match:
A0A1Z3GC64 (Ent-kaurene synthase 5, chloroplastic OS=Isodon rubescens OX=587669 GN=KSL5 PE=1 SV=1)
HSP 1 Score: 689.1 bits (1777), Expect = 5.1e-197
Identity = 360/750 (48.00%), Postives = 484/750 (64.53%), Query Frame = 0
Query: 3 CFEGTKERIKKMFEKVELSVSAYDTAWMAMVPTPNCLKTPLFPECLNWLLDNQLPDGSWG 62
CFE TK RI K+F K ELSVS YDTAW+AMVP+P + P FP CLNWLL+NQ DGSW
Sbjct: 45 CFEETKGRIAKLFHKNELSVSTYDTAWVAMVPSPTSSEEPCFPACLNWLLENQCHDGSWA 104
Query: 63 LLHNYPILTKATLLSTFASILALKRWDVGQEHMIKGLQFIESNLDSATDEKQHSPIGFDI 122
H++ +L K L ST A ILALK+W VG+E + +GL F+E N SAT++ Q +P+GFDI
Sbjct: 105 RPHHHHMLKKDVLSSTLACILALKKWGVGEEQINRGLHFVELNFASATEKGQITPMGFDI 164
Query: 123 IFSGMIEYAKTLDLSLPLPQSNIDALIRRRELELSR---SNSEGKKAYLAYVSEGLGKSQ 182
IF M++ A+ L L+L L + ++ LI +R+LEL R SNS K+ Y A+++EG+GK Q
Sbjct: 165 IFPAMLDNARGLSLNLQLEPTTLNDLIYKRDLELKRCNQSNSAEKEVYWAHIAEGMGKLQ 224
Query: 183 DWGMVMNFQRKNGSLFNSPSTTAAAFMNLNNDSCLDYLHSLVQKFGNAVPTIYPLDIYAQ 242
DW VM +QRKNGSLFN PSTTAAAF L N CL+YL ++KFG+AVP +YPLDIY+Q
Sbjct: 225 DWESVMKYQRKNGSLFNCPSTTAAAFTALRNSDCLNYLRLALEKFGSAVPAVYPLDIYSQ 284
Query: 243 LCMVDNLQTLGIDRHFKEEIGSLLDKTYRHWLKGDENILLDASTCAMAFRMLHQ------ 302
LC VDNL+ LGI R+F EI S+LD+TYR WL+GDE I +DASTCA+AFR L
Sbjct: 285 LCTVDNLERLGISRYFLTEIQSVLDETYRSWLQGDEEIFMDASTCALAFRTLRMNGYNVT 344
Query: 303 ---LAQFSEDRFSDCLGGYLKDIGSILELFKASQITICPEESVLD--------------- 362
+ + ++ FS G + DI + LE+++AS++ + PEE LD
Sbjct: 345 SDPITKILQECFSSSFRGNMTDINTTLEIYRASELILYPEERDLDQHNLRLKTFLEQELS 404
Query: 363 ------------NINTWTN----------LNRIFRKLS-----------TLVNFSCLNLG 422
NIN N ++R+ ++ + ++ N G
Sbjct: 405 SNGFIQSCQLGRNINAEVNQAIEYPFYAIMDRMAKRKNIENYNIDNTRILKTSYRSPNFG 464
Query: 423 HQDFLRLAMEEFYGCQCIQRQEKKDLERWVIENKLEKL-LPRHRVVYCYFTGAALITSPE 482
++DFL L++E+F CQ I R+E ++LERWVIEN+L++L R + YCYF+ AA I SPE
Sbjct: 465 NKDFLSLSVEDFNRCQVIHREELRELERWVIENRLDELKFARSKAAYCYFSAAATIFSPE 524
Query: 483 LTEARMSFGKSGVLTTVLDAFFDDWGSQDERTNLVQAAERWDVSNEMEFCSEEVEIIFKA 542
L++ARMS+ K+GVLTTV+D FFD GS +E NL+Q E WDV + CS V+IIF A
Sbjct: 525 LSDARMSWAKNGVLTTVVDDFFDVGGSVEELKNLIQLVELWDVDVSTQCCSPNVQIIFSA 584
Query: 543 LRRAVDEISNAALTWQGRSVRSHVIDTWVELIKSMAMEADWSSNKTVPSMDEYIETSAVS 602
L+ + EI++ QGRS+ H+I W++L+ SM E + +K+ P+MDEY+ + VS
Sbjct: 585 LKHTICEIADKGFKLQGRSITDHIISIWLDLLYSMMKETELGIDKSFPTMDEYMSNAYVS 644
Query: 603 FALGPIVLPALYFIGPKISEEMVGSFEYHNLFMLMSIAGRLLNDIKTIEKEAGEGKHNAF 662
FALGPIVLPALY +GPK+SEEMV EYH LF LMS GRLLNDI+ E+E +GK +A
Sbjct: 645 FALGPIVLPALYLVGPKLSEEMVNHSEYHTLFKLMSTCGRLLNDIRGYERELKDGKISAV 704
Query: 663 SLRMLDGGVHITKEEAIRAIQVDIDRATRKLLRLVLQVEDSTIPRVCKDLFWRVNCAVHV 692
SL +++ G IT E AI ++ I+R R+LLRLVL+ S +P+ CK LFW + VH+
Sbjct: 705 SLYIMNNGGEITTEAAISEMRSWIERDRRELLRLVLEENKSVLPKACKKLFWHMCTVVHL 764
BLAST of Spg021407 vs. ExPASy Swiss-Prot
Match:
A0A075FBT3 (Ent-kaurene synthase, chloroplastic (Fragment) OS=Marrubium vulgare OX=41230 GN=EKS PE=1 SV=1)
HSP 1 Score: 676.4 bits (1744), Expect = 3.4e-193
Identity = 348/730 (47.67%), Postives = 473/730 (64.79%), Query Frame = 0
Query: 1 MQCFEGTKERIKKMFEKVELSVSAYDTAWMAMVPTPNCLKTPLFPECLNWLLDNQLPDGS 60
+QCFE TK RI K+ K ELSVS YDTAW+AMVP+PN P FP+CL WLL+NQ DGS
Sbjct: 24 VQCFEDTKARIVKLIHKPELSVSTYDTAWVAMVPSPNSSNEPCFPDCLAWLLENQCCDGS 83
Query: 61 WGLLHNYPILTKATLLSTFASILALKRWDVGQEHMIKGLQFIESNLDSATDEKQHSPIGF 120
W H++P L K L ST A ILALK+W VG+E + KG +FIE N SAT++ Q SP GF
Sbjct: 84 WACPHHHPFLKKDVLSSTLACILALKKWGVGEEQINKGARFIEVNFASATEKSQISPTGF 143
Query: 121 DIIFSGMIEYAKTLDLSLPLPQSNIDALIRRRELELSRSNSEGKKAYLAYVSEGLGKSQD 180
DIIF M++YA+ L L L L + D LI RR+LEL R ++AYLAY++EG+GK QD
Sbjct: 144 DIIFPAMLDYARDLFLDLRLEPTTFDNLIFRRDLELKRCYESHREAYLAYIAEGMGKLQD 203
Query: 181 WGMVMNFQRKNGSLFNSPSTTAAAFMNLNNDSCLDYLHSLVQKFGNAVPTIYPLDIYAQL 240
W VM +QRKNGSLFNSPSTTAAAF+ L N CL YLHS ++KFGNAVP YPLD+Y++L
Sbjct: 204 WESVMKYQRKNGSLFNSPSTTAAAFIALPNSGCLSYLHSALKKFGNAVPAAYPLDVYSRL 263
Query: 241 CMVDNLQTLGIDRHFKEEIGSLLDKTYRHWLKGDENILLDASTCAMAFRMLHQLA-QFSE 300
VDNL++LGI R+F++EI +LD+TYR WL+G E I LDASTCA+AFR L +
Sbjct: 264 RTVDNLESLGISRYFQKEIQQVLDETYRWWLQGSEEIFLDASTCALAFRTLRMNGYNVTS 323
Query: 301 DRFS----DCLGGYLKDIGSILELFKASQITICPEESVLDNINT---------------- 360
D + D G +KDIG+ LEL++AS+ + P+E L+ N
Sbjct: 324 DAITKLLPDSFCGNMKDIGTTLELYRASEFILYPDEKDLEQQNLRLKDILEQELSSGFIH 383
Query: 361 -----------WTNLNRIFRKLS-----------TLVNFSCLNLGHQDFLRLAMEEFYGC 420
+ ++R+ ++ + ++ N ++DFL L++E+F C
Sbjct: 384 SEVNRALNYPFYAIMDRVAKRRNIEHYNFDNTRILKTSYCSPNFANKDFLFLSVEDFNNC 443
Query: 421 QCIQRQEKKDLERWVIENKLEKL-LPRHRVVYCYFTGAALITSPELTEARMSFGKSGVLT 480
Q + R+E K RWV EN+L++L R + YCYF+ AA +P+L++ARMS+ K+GVLT
Sbjct: 444 QAMHREELKGKMRWVTENRLDELKFARSKSAYCYFSAAATFFAPDLSDARMSWAKNGVLT 503
Query: 481 TVLDAFFDDWGSQDERTNLVQAAERWDVSNEMEFCSEEVEIIFKALRRAVDEISNAALTW 540
TV+D FFD GS++E L++ E WD+ E S+ V+IIF +L+R + EI +
Sbjct: 504 TVVDDFFDVGGSEEELKQLIRLVEIWDLDAITECSSQNVQIIFSSLKRTISEIGDKGFKL 563
Query: 541 QGRSVRSHVIDTWVELIKSMAMEADWSSNKTVPSMDEYIETSAVSFALGPIVLPALYFIG 600
QGRSV +H+I+ W++L+ SM EA+W+ + P MD+YI + VSFALGPIVLP LY +G
Sbjct: 564 QGRSVTNHIIEIWLDLLYSMMKEAEWARDNHAPPMDDYISNAYVSFALGPIVLPCLYLVG 623
Query: 601 PKISEEMVGSFEYHNLFMLMSIAGRLLNDIKTIEKEAGEGKHNAFSLRMLDGGVHITKEE 660
PK+SEEMV E H LF LMS GRLLNDI+T E+E +GK NA L M++ G +KE
Sbjct: 624 PKLSEEMVRHSECHTLFRLMSTCGRLLNDIQTCERELKDGKLNAIPLYMINSGGETSKEA 683
Query: 661 AIRAIQVDIDRATRKLLRLVLQVEDSTIPRVCKDLFWRVNCAVHVFYRKDDGFTTLGLMD 687
A + ++ IDR ++LLRLVL E S +P+ CK+LFW ++ +H+FY KDDGFT+ L+
Sbjct: 684 ATQEMKSLIDRQRQELLRLVLTREGSLLPKPCKELFWHMSTVLHLFYCKDDGFTSQDLIK 743
BLAST of Spg021407 vs. ExPASy Swiss-Prot
Match:
A0A3G1QTS7 (Ent-kaurene synthase 1, chloroplastic OS=Isodon eriocalyx OX=662907 GN=KS1 PE=1 SV=1)
HSP 1 Score: 676.0 bits (1743), Expect = 4.5e-193
Identity = 356/750 (47.47%), Postives = 479/750 (63.87%), Query Frame = 0
Query: 3 CFEGTKERIKKMFEKVELSVSAYDTAWMAMVPTPNCLKTPLFPECLNWLLDNQLPDGSWG 62
CFE TK RI K+F K ELSVS YDTAW+AMVP+P + P FP CLNWLL+NQ DGSW
Sbjct: 45 CFEETKGRIAKLFHKNELSVSTYDTAWVAMVPSPTSSEEPCFPACLNWLLENQCHDGSWA 104
Query: 63 LLHNYPILTKATLLSTFASILALKRWDVGQEHMIKGLQFIESNLDSATDEKQHSPIGFDI 122
H++ +L K L ST A ILALK+W VG+E + +GL F+E N SAT++ Q +P+GFDI
Sbjct: 105 RPHHHHMLKKDVLSSTLACILALKKWGVGEEQINRGLHFVELNFASATEKGQITPMGFDI 164
Query: 123 IFSGMIEYAKTLDLSLPLPQSNIDALIRRRELELSR---SNSEGKKAYLAYVSEGLGKSQ 182
IF M++ A+ L L+L L + ++ LI +R+LEL R SNS K+ Y A+++EG+GK
Sbjct: 165 IFPAMLDNARGLSLNLQLEPTMLNDLIYKRDLELKRCNQSNSAEKEVYWAHIAEGMGKLH 224
Query: 183 DWGMVMNFQRKNGSLFNSPSTTAAAFMNLNNDSCLDYLHSLVQKFGNAVPTIYPLDIYAQ 242
DW VM +QRKNGSLFN PSTTAAAF L N CL+YL ++KFG+AVP +YPLDIY+Q
Sbjct: 225 DWESVMKYQRKNGSLFNCPSTTAAAFTALRNSDCLNYLCLALEKFGSAVPAVYPLDIYSQ 284
Query: 243 LCMVDNLQTLGIDRHFKEEIGSLLDKTYRHWLKGDENILLDASTCAMAFRMLHQ------ 302
LC V NL+ LGI R+F EI S+LD+TYR WL+GDE I +DASTCA+AFR L
Sbjct: 285 LCTVGNLERLGISRYFLTEIQSVLDETYRSWLQGDEEIFMDASTCALAFRTLRMNGYNVT 344
Query: 303 ---LAQFSEDRFSDCLGGYLKDIGSILELFKASQITICPEESVL---------------- 362
+ + ++ FS G + D + LE+++AS++ + PEE L
Sbjct: 345 SDPITKILQECFSSSFRGNMTDNNTTLEIYRASELILYPEERDLVQHNLRLKTFLEQELS 404
Query: 363 -----------DNINTWTN----------LNRIFRKLS-----------TLVNFSCLNLG 422
NIN N ++R+ ++ + ++ N G
Sbjct: 405 SNGFIQSCQLGRNINAEVNQAIEYPFYAIMDRMAKRKNIENYNIDNTRILKTSYRSPNFG 464
Query: 423 HQDFLRLAMEEFYGCQCIQRQEKKDLERWVIENKLEKL-LPRHRVVYCYFTGAALITSPE 482
++DFL L++E+F CQ I R+E ++LERWVIEN+L++L R + YCYF+ AA I SPE
Sbjct: 465 NKDFLSLSVEDFNRCQVIHREELRELERWVIENRLDELKFARSKAAYCYFSAAATIFSPE 524
Query: 483 LTEARMSFGKSGVLTTVLDAFFDDWGSQDERTNLVQAAERWDVSNEMEFCSEEVEIIFKA 542
L++ARMS+ K+GVLTTV+D FFD GS +E NL+Q E WDV + CS V+IIF A
Sbjct: 525 LSDARMSWAKNGVLTTVVDDFFDVGGSVEELKNLIQLVELWDVDVSTQCCSPNVQIIFSA 584
Query: 543 LRRAVDEISNAALTWQGRSVRSHVIDTWVELIKSMAMEADWSSNKTVPSMDEYIETSAVS 602
L+ + EI++ QGRS+ H+I W++L+ SM E + +K+ P+MDEY+ + VS
Sbjct: 585 LKHTICEIADKGFKLQGRSITDHIISIWLDLLYSMMKETELGIDKSFPTMDEYMSNAYVS 644
Query: 603 FALGPIVLPALYFIGPKISEEMVGSFEYHNLFMLMSIAGRLLNDIKTIEKEAGEGKHNAF 662
FALGPIVLPALY +GPK+SEEMV EYH LF LMS GRLLNDI+ E+E +GK +A
Sbjct: 645 FALGPIVLPALYLVGPKLSEEMVNHSEYHTLFKLMSTCGRLLNDIRGYERELKDGKISAV 704
Query: 663 SLRMLDGGVHITKEEAIRAIQVDIDRATRKLLRLVLQVEDSTIPRVCKDLFWRVNCAVHV 692
SL +++ G IT E AI ++ I+R R+LLRLVL+ S +P+ CK LFW + VHV
Sbjct: 705 SLYIMNNGGEITTEAAISEMRSWIERDRRELLRLVLEENKSVLPKACKKLFWHMCTVVHV 764
BLAST of Spg021407 vs. ExPASy Swiss-Prot
Match:
Q9XEH9 (Ent-kaurene synthase 1, chloroplastic OS=Stevia rebaudiana OX=55670 GN=KS1-1 PE=1 SV=1)
HSP 1 Score: 676.0 bits (1743), Expect = 4.5e-193
Identity = 349/736 (47.42%), Postives = 488/736 (66.30%), Query Frame = 0
Query: 7 TKERIKKMFEKVELSVSAYDTAWMAMVPTPNCLKTPLFPECLNWLLDNQLPDGSWGLL-- 66
TKERI+K+F+ VE+SVS+YDTAW+AMVP+PN K+P FPECLNWL++NQL DGSWGL+
Sbjct: 45 TKERIQKLFKNVEISVSSYDTAWVAMVPSPNSPKSPCFPECLNWLINNQLNDGSWGLVNH 104
Query: 67 ---HNYPILTKATLLSTFASILALKRWDVGQEHMIKGLQFIESNLDSATDEKQHSPIGFD 126
HN+P+L K +L ST A I+ALKRW+VG++ + KGL FIESNL SATD+ Q SPIGFD
Sbjct: 105 THNHNHPLL-KDSLSSTLACIVALKRWNVGEDQINKGLSFIESNLASATDKSQPSPIGFD 164
Query: 127 IIFSGMIEYAKTLDLSLPLPQSNIDALIRRRELELSRSNSEGKKAYLAYVSEGLGKSQDW 186
IIF G++EYAK LD++L Q++ ++ +RELE R +S YLAY+SEGLG DW
Sbjct: 165 IIFPGLLEYAKNLDINLLSKQTDFSLMLHKRELEQKRCHSNEIDGYLAYISEGLGNLYDW 224
Query: 187 GMVMNFQRKNGSLFNSPSTTAAAFMNLNNDSCLDYLHSLVQKFGNAVPTIYPLDIYAQLC 246
MV +Q KNGS+FNSPS TAAAF+N N CL+YL+SL+ KFGNAVPT+YPLD+Y +L
Sbjct: 225 NMVKKYQMKNGSVFNSPSATAAAFINHQNPGCLNYLNSLLDKFGNAVPTVYPLDLYIRLS 284
Query: 247 MVDNLQTLGIDRHFKEEIGSLLDKTYRHWLKGDENILLDASTCAMAFRMLH--------- 306
MVD ++ LGI HF+ EI ++LD+TYR W++ DE I +D TCA+AFR+L
Sbjct: 285 MVDTIERLGISHHFRVEIKNVLDETYRCWVERDEQIFMDVVTCALAFRLLRIHGYKVSPD 344
Query: 307 QLAQFSED-RFSDCLGG----------YLKDIGSILELFKASQI---------------- 366
QLA+ + + F D Y +D+ S ++ K++
Sbjct: 345 QLAEITNELAFKDEYAALETYHASQILYQEDLSSGKQILKSADFLKGILSTDSNRLSKLI 404
Query: 367 -------TICPEESVLDNINTWTNLNRIFRKLSTLV---NFSCLNLGHQDFLRLAMEEFY 426
P + L+ INT N+ +++ +T + + N+ + +LRLA+E+FY
Sbjct: 405 HKEVENALKFPINTGLERINTRRNI-QLYNVDNTRILKTTYHSSNISNTYYLRLAVEDFY 464
Query: 427 GCQCIQRQEKKDLERWVIENKLEKL-LPRHRVVYCYFTGAALITSPELTEARMSFGKSGV 486
CQ I R+E K LERWV++NKL++L R + YCYF+ AA ++SPEL++AR+S+ K+G+
Sbjct: 465 TCQSIYREELKGLERWVVQNKLDQLKFARQKTAYCYFSVAATLSSPELSDARISWAKNGI 524
Query: 487 LTTVLDAFFDDWGSQDERTNLVQAAERWDVSNEMEFCSEEVEIIFKALRRAVDEISNAAL 546
LTTV+D FFD G+ DE TNL+Q E+W+V + + CSE V I+F AL+ A+ I + A
Sbjct: 525 LTTVVDDFFDIGGTIDELTNLIQCVEKWNVDVDKDCCSEHVRILFLALKDAICWIGDEAF 584
Query: 547 TWQGRSVRSHVIDTWVELIKSMAMEADWSSNKTVPSMDEYIETSAVSFALGPIVLPALYF 606
WQ R V SHVI TW+EL+ SM EA W+ + VP+++EY+E + VSFALGPIV PA+YF
Sbjct: 585 KWQARDVTSHVIQTWLELMNSMLREAIWTRDAYVPTLNEYMENAYVSFALGPIVKPAIYF 644
Query: 607 IGPKISEEMVGSFEYHNLFMLMSIAGRLLNDIKTIEKEAGEGKHNAFSLRMLDGGVHITK 666
+GPK+SEE+V S EYHNLF LMS GRLLNDI + ++E EGK NA +L + +G +
Sbjct: 645 VGPKLSEEIVESSEYHNLFKLMSTQGRLLNDIHSFKREFKEGKLNAVALHLSNGESGKVE 704
Query: 667 EEAIRAIQVDIDRATRKLLRLVLQVEDSTIPRVCKDLFWRVNCAVHVFYRKDDGFTTLGL 691
EE + + + I ++L++L+ + S +PR CKD FW + ++ FY DDGFT +
Sbjct: 705 EEVVEEMMMMIKNKRKELMKLIFEENGSIVPRACKDAFWNMCHVLNFFYANDDGFTGNTI 764
BLAST of Spg021407 vs. ExPASy TrEMBL
Match:
A0A6J1D2F0 (ent-kaur-16-ene synthase, chloroplastic isoform X2 OS=Momordica charantia OX=3673 GN=LOC111016726 PE=4 SV=1)
HSP 1 Score: 917.9 bits (2371), Expect = 2.5e-263
Identity = 454/744 (61.02%), Postives = 558/744 (75.00%), Query Frame = 0
Query: 3 CFEGTKERIKKMFEKVELSVSAYDTAWMAMVPTPNCLKTPLFPECLNWLLDNQLPDGSWG 62
CFE TKERIKKMF KVELSVSAYDTAW+AMVP+PN L PLFPEC+NW+LDNQ PDGSWG
Sbjct: 85 CFEETKERIKKMFNKVELSVSAYDTAWVAMVPSPNSLNDPLFPECINWVLDNQHPDGSWG 144
Query: 63 LLHNYPILTKATLLSTFASILALKRWDVGQEHMIKGLQFIESNLDSATDEKQHSPIGFDI 122
++HN+ +L KA+LLST A L LKRW++G EHM K L FI+SN+ SATDEKQ+SP+GFDI
Sbjct: 145 IIHNHHLLMKASLLSTLACALTLKRWNIGHEHMSKALNFIKSNIVSATDEKQYSPVGFDI 204
Query: 123 IFSGMIEYAKTLDLSLPLPQSNIDALIRRRELELSRSNSEGKKAYLAYVSEGLGKSQDWG 182
IF GMIEYAK LDL+LPL +N+DAL++++EL+L RSNSEG KAYLAYVSEG+G+ +DW
Sbjct: 205 IFPGMIEYAKDLDLNLPLAPANVDALLQKKELDLRRSNSEGGKAYLAYVSEGIGRLRDWE 264
Query: 183 MVMNFQRKNGSLFNSPSTTAAAFMNLNNDSCLDYLHSLVQKFGNAVPTIYPLDIYAQLCM 242
MVM +QRKNGSLFNSPSTTA AFM+ N+D C +YLHSL+QKF N+VPTIYPLDIYA+LCM
Sbjct: 265 MVMQYQRKNGSLFNSPSTTAVAFMHGNDDGCFNYLHSLLQKFDNSVPTIYPLDIYARLCM 324
Query: 243 VDNLQTLGIDRHFKEEIGSLLDKTYRHWLKGDENILLDASTCAMAFRMLH---------Q 302
VDNLQ LGIDRHFKEEI S+LD+TYR W++G+E+I LDASTCAMAFRML Q
Sbjct: 325 VDNLQRLGIDRHFKEEIRSVLDETYRCWMQGEEDIFLDASTCAMAFRMLRLGGYDVSSDQ 384
Query: 303 LAQFSEDRFSDCLGGYLKDIGSILELFKASQITICPEESVLDNINTWTN----------- 362
L QFSED FS+CLGGYLKD GS LELFKASQI I P+ES+L+NIN+WTN
Sbjct: 385 LTQFSEDLFSNCLGGYLKDFGSSLELFKASQIIIYPDESILENINSWTNSFLSHGLSSGS 444
Query: 363 -------------------------LNRIFRKLS-----------TLVNFSCLNLGHQDF 422
L R+ K + + ++CLN GHQDF
Sbjct: 445 VHSDRPDRLLKQEAINALEFTYSNTLERLLNKRAMENYNIDIVRISKTTYTCLNFGHQDF 504
Query: 423 LRLAMEEFYGCQCIQRQEKKDLERWVIENKLEKL-LPRHRVVYCYFTGAALITSPELTEA 482
L LA+E+F Q I +E K+LERWV+EN+L+KL R ++ YCYF+ AA +TSPEL +A
Sbjct: 505 LELAVEDFNILQSIHHKELKELERWVVENRLDKLKFARQKLAYCYFSAAATLTSPELRDA 564
Query: 483 RMSFGKSGVLTTVLDAFFDDWGSQDERTNLVQAAERWDVSNEMEFCSEEVEIIFKALRRA 542
R+S+ K+GVLTTV+D FFD GS+ E NL+Q E+WDV+ E ++CS+EVEIIF AL
Sbjct: 565 RLSWAKNGVLTTVVDDFFDVGGSEGELINLIQLVEKWDVNGEADYCSKEVEIIFLALHGT 624
Query: 543 VDEISNAALTWQGRSVRSHVIDTWVELIKSMAMEADWSSNKTVPSMDEYIETSAVSFALG 602
V EI AL WQGRSV +VID W+ L+KSM EA+W NK P MDEY+E +SFALG
Sbjct: 625 VCEIEKRALPWQGRSVMRNVIDIWLALLKSMRKEAEWLKNKIAPPMDEYMENGYISFALG 684
Query: 603 PIVLPALYFIGPKISEEMVGSFEYHNLFMLMSIAGRLLNDIKTIEKEAGEGKHNAFSLRM 662
PIVLPAL+F+GPK+ EE++GS EYH LF LMS GRLLND ++ E+E+ EGK NA SL M
Sbjct: 685 PIVLPALFFVGPKLPEEVIGSCEYHKLFKLMSTCGRLLNDTQSFERESSEGKLNAMSLCM 744
Query: 663 LDGGVHITKEEAIRAIQVDIDRATRKLLRLVLQVEDSTIPRVCKDLFWRVNCAVHVFYRK 690
++ G TKEEA AI+ +++R R+LLRLVLQ ++ST+PR CKDLFW+++C VH+FYRK
Sbjct: 745 INAGGDFTKEEASEAIKGNVERTRRELLRLVLQEKNSTVPRACKDLFWKMSCVVHLFYRK 804
BLAST of Spg021407 vs. ExPASy TrEMBL
Match:
A0A6J1D2L1 (ent-kaur-16-ene synthase, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016726 PE=4 SV=1)
HSP 1 Score: 912.9 bits (2358), Expect = 8.0e-262
Identity = 454/746 (60.86%), Postives = 558/746 (74.80%), Query Frame = 0
Query: 3 CFEGTKERIKKMFEKVELSVSAYDTAWMAMVPTPNCLKTPLFPECLNWLLDNQLPDGSWG 62
CFE TKERIKKMF KVELSVSAYDTAW+AMVP+PN L PLFPEC+NW+LDNQ PDGSWG
Sbjct: 85 CFEETKERIKKMFNKVELSVSAYDTAWVAMVPSPNSLNDPLFPECINWVLDNQHPDGSWG 144
Query: 63 LLHNYPILTKATLLSTFASILALKRWDVGQEHMIKGLQFIESNLDSATDEKQHSPIGFDI 122
++HN+ +L KA+LLST A L LKRW++G EHM K L FI+SN+ SATDEKQ+SP+GFDI
Sbjct: 145 IIHNHHLLMKASLLSTLACALTLKRWNIGHEHMSKALNFIKSNIVSATDEKQYSPVGFDI 204
Query: 123 IFSGMIEYAKTLDLSLPLPQSNIDALIRRRELEL--SRSNSEGKKAYLAYVSEGLGKSQD 182
IF GMIEYAK LDL+LPL +N+DAL++++EL+L RSNSEG KAYLAYVSEG+G+ +D
Sbjct: 205 IFPGMIEYAKDLDLNLPLAPANVDALLQKKELDLRSCRSNSEGGKAYLAYVSEGIGRLRD 264
Query: 183 WGMVMNFQRKNGSLFNSPSTTAAAFMNLNNDSCLDYLHSLVQKFGNAVPTIYPLDIYAQL 242
W MVM +QRKNGSLFNSPSTTA AFM+ N+D C +YLHSL+QKF N+VPTIYPLDIYA+L
Sbjct: 265 WEMVMQYQRKNGSLFNSPSTTAVAFMHGNDDGCFNYLHSLLQKFDNSVPTIYPLDIYARL 324
Query: 243 CMVDNLQTLGIDRHFKEEIGSLLDKTYRHWLKGDENILLDASTCAMAFRMLH-------- 302
CMVDNLQ LGIDRHFKEEI S+LD+TYR W++G+E+I LDASTCAMAFRML
Sbjct: 325 CMVDNLQRLGIDRHFKEEIRSVLDETYRCWMQGEEDIFLDASTCAMAFRMLRLGGYDVSS 384
Query: 303 -QLAQFSEDRFSDCLGGYLKDIGSILELFKASQITICPEESVLDNINTWTN--------- 362
QL QFSED FS+CLGGYLKD GS LELFKASQI I P+ES+L+NIN+WTN
Sbjct: 385 DQLTQFSEDLFSNCLGGYLKDFGSSLELFKASQIIIYPDESILENINSWTNSFLSHGLSS 444
Query: 363 ---------------------------LNRIFRKLS-----------TLVNFSCLNLGHQ 422
L R+ K + + ++CLN GHQ
Sbjct: 445 GSVHSDRPDRLLKQEAINALEFTYSNTLERLLNKRAMENYNIDIVRISKTTYTCLNFGHQ 504
Query: 423 DFLRLAMEEFYGCQCIQRQEKKDLERWVIENKLEKL-LPRHRVVYCYFTGAALITSPELT 482
DFL LA+E+F Q I +E K+LERWV+EN+L+KL R ++ YCYF+ AA +TSPEL
Sbjct: 505 DFLELAVEDFNILQSIHHKELKELERWVVENRLDKLKFARQKLAYCYFSAAATLTSPELR 564
Query: 483 EARMSFGKSGVLTTVLDAFFDDWGSQDERTNLVQAAERWDVSNEMEFCSEEVEIIFKALR 542
+AR+S+ K+GVLTTV+D FFD GS+ E NL+Q E+WDV+ E ++CS+EVEIIF AL
Sbjct: 565 DARLSWAKNGVLTTVVDDFFDVGGSEGELINLIQLVEKWDVNGEADYCSKEVEIIFLALH 624
Query: 543 RAVDEISNAALTWQGRSVRSHVIDTWVELIKSMAMEADWSSNKTVPSMDEYIETSAVSFA 602
V EI AL WQGRSV +VID W+ L+KSM EA+W NK P MDEY+E +SFA
Sbjct: 625 GTVCEIEKRALPWQGRSVMRNVIDIWLALLKSMRKEAEWLKNKIAPPMDEYMENGYISFA 684
Query: 603 LGPIVLPALYFIGPKISEEMVGSFEYHNLFMLMSIAGRLLNDIKTIEKEAGEGKHNAFSL 662
LGPIVLPAL+F+GPK+ EE++GS EYH LF LMS GRLLND ++ E+E+ EGK NA SL
Sbjct: 685 LGPIVLPALFFVGPKLPEEVIGSCEYHKLFKLMSTCGRLLNDTQSFERESSEGKLNAMSL 744
Query: 663 RMLDGGVHITKEEAIRAIQVDIDRATRKLLRLVLQVEDSTIPRVCKDLFWRVNCAVHVFY 690
M++ G TKEEA AI+ +++R R+LLRLVLQ ++ST+PR CKDLFW+++C VH+FY
Sbjct: 745 CMINAGGDFTKEEASEAIKGNVERTRRELLRLVLQEKNSTVPRACKDLFWKMSCVVHLFY 804
BLAST of Spg021407 vs. ExPASy TrEMBL
Match:
A0A6J1D1G1 (ent-kaur-16-ene synthase, chloroplastic isoform X3 OS=Momordica charantia OX=3673 GN=LOC111016726 PE=4 SV=1)
HSP 1 Score: 912.9 bits (2358), Expect = 8.0e-262
Identity = 454/746 (60.86%), Postives = 558/746 (74.80%), Query Frame = 0
Query: 3 CFEGTKERIKKMFEKVELSVSAYDTAWMAMVPTPNCLKTPLFPECLNWLLDNQLPDGSWG 62
CFE TKERIKKMF KVELSVSAYDTAW+AMVP+PN L PLFPEC+NW+LDNQ PDGSWG
Sbjct: 72 CFEETKERIKKMFNKVELSVSAYDTAWVAMVPSPNSLNDPLFPECINWVLDNQHPDGSWG 131
Query: 63 LLHNYPILTKATLLSTFASILALKRWDVGQEHMIKGLQFIESNLDSATDEKQHSPIGFDI 122
++HN+ +L KA+LLST A L LKRW++G EHM K L FI+SN+ SATDEKQ+SP+GFDI
Sbjct: 132 IIHNHHLLMKASLLSTLACALTLKRWNIGHEHMSKALNFIKSNIVSATDEKQYSPVGFDI 191
Query: 123 IFSGMIEYAKTLDLSLPLPQSNIDALIRRRELEL--SRSNSEGKKAYLAYVSEGLGKSQD 182
IF GMIEYAK LDL+LPL +N+DAL++++EL+L RSNSEG KAYLAYVSEG+G+ +D
Sbjct: 192 IFPGMIEYAKDLDLNLPLAPANVDALLQKKELDLRSCRSNSEGGKAYLAYVSEGIGRLRD 251
Query: 183 WGMVMNFQRKNGSLFNSPSTTAAAFMNLNNDSCLDYLHSLVQKFGNAVPTIYPLDIYAQL 242
W MVM +QRKNGSLFNSPSTTA AFM+ N+D C +YLHSL+QKF N+VPTIYPLDIYA+L
Sbjct: 252 WEMVMQYQRKNGSLFNSPSTTAVAFMHGNDDGCFNYLHSLLQKFDNSVPTIYPLDIYARL 311
Query: 243 CMVDNLQTLGIDRHFKEEIGSLLDKTYRHWLKGDENILLDASTCAMAFRMLH-------- 302
CMVDNLQ LGIDRHFKEEI S+LD+TYR W++G+E+I LDASTCAMAFRML
Sbjct: 312 CMVDNLQRLGIDRHFKEEIRSVLDETYRCWMQGEEDIFLDASTCAMAFRMLRLGGYDVSS 371
Query: 303 -QLAQFSEDRFSDCLGGYLKDIGSILELFKASQITICPEESVLDNINTWTN--------- 362
QL QFSED FS+CLGGYLKD GS LELFKASQI I P+ES+L+NIN+WTN
Sbjct: 372 DQLTQFSEDLFSNCLGGYLKDFGSSLELFKASQIIIYPDESILENINSWTNSFLSHGLSS 431
Query: 363 ---------------------------LNRIFRKLS-----------TLVNFSCLNLGHQ 422
L R+ K + + ++CLN GHQ
Sbjct: 432 GSVHSDRPDRLLKQEAINALEFTYSNTLERLLNKRAMENYNIDIVRISKTTYTCLNFGHQ 491
Query: 423 DFLRLAMEEFYGCQCIQRQEKKDLERWVIENKLEKL-LPRHRVVYCYFTGAALITSPELT 482
DFL LA+E+F Q I +E K+LERWV+EN+L+KL R ++ YCYF+ AA +TSPEL
Sbjct: 492 DFLELAVEDFNILQSIHHKELKELERWVVENRLDKLKFARQKLAYCYFSAAATLTSPELR 551
Query: 483 EARMSFGKSGVLTTVLDAFFDDWGSQDERTNLVQAAERWDVSNEMEFCSEEVEIIFKALR 542
+AR+S+ K+GVLTTV+D FFD GS+ E NL+Q E+WDV+ E ++CS+EVEIIF AL
Sbjct: 552 DARLSWAKNGVLTTVVDDFFDVGGSEGELINLIQLVEKWDVNGEADYCSKEVEIIFLALH 611
Query: 543 RAVDEISNAALTWQGRSVRSHVIDTWVELIKSMAMEADWSSNKTVPSMDEYIETSAVSFA 602
V EI AL WQGRSV +VID W+ L+KSM EA+W NK P MDEY+E +SFA
Sbjct: 612 GTVCEIEKRALPWQGRSVMRNVIDIWLALLKSMRKEAEWLKNKIAPPMDEYMENGYISFA 671
Query: 603 LGPIVLPALYFIGPKISEEMVGSFEYHNLFMLMSIAGRLLNDIKTIEKEAGEGKHNAFSL 662
LGPIVLPAL+F+GPK+ EE++GS EYH LF LMS GRLLND ++ E+E+ EGK NA SL
Sbjct: 672 LGPIVLPALFFVGPKLPEEVIGSCEYHKLFKLMSTCGRLLNDTQSFERESSEGKLNAMSL 731
Query: 663 RMLDGGVHITKEEAIRAIQVDIDRATRKLLRLVLQVEDSTIPRVCKDLFWRVNCAVHVFY 690
M++ G TKEEA AI+ +++R R+LLRLVLQ ++ST+PR CKDLFW+++C VH+FY
Sbjct: 732 CMINAGGDFTKEEASEAIKGNVERTRRELLRLVLQEKNSTVPRACKDLFWKMSCVVHLFY 791
BLAST of Spg021407 vs. ExPASy TrEMBL
Match:
A0A1S4DWJ8 (ent-kaur-16-ene synthase, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC103489622 PE=4 SV=1)
HSP 1 Score: 884.0 bits (2283), Expect = 4.0e-253
Identity = 440/743 (59.22%), Postives = 553/743 (74.43%), Query Frame = 0
Query: 4 FEGTKERIKKMFEKVELSVSAYDTAWMAMVPTPNCLKTPLFPECLNWLLDNQLPDGSWGL 63
FE TKERI+K+F+ VELSVSAYDTAW+AMVP+PN L PLFPEC+NW+LD+Q PDGSWG+
Sbjct: 37 FEDTKERIQKLFKNVELSVSAYDTAWVAMVPSPNSLNKPLFPECINWVLDHQNPDGSWGI 96
Query: 64 LHNYPILTKATLLSTFASILALKRWDVGQEHMIKGLQFIESNLDSATDEKQHSPIGFDII 123
LH++ ++ KATLLST A +L LKRW++G +HM K L FI+SN+ SATDE Q SP+GFDII
Sbjct: 97 LHDHQLVMKATLLSTLACVLTLKRWNIGHDHMSKALSFIKSNIASATDENQRSPVGFDII 156
Query: 124 FSGMIEYAKTLDLSLPLPQSNIDALIRRRELELSRSNSEGKKAYLAYVSEGLGKSQDWGM 183
F GMIEYAK L+L+LPL N+DAL++++ELEL RSNSEG KAYLAYVSEG+GK QDW M
Sbjct: 157 FPGMIEYAKDLNLNLPLASMNVDALVQKKELEL-RSNSEGGKAYLAYVSEGIGKLQDWDM 216
Query: 184 VMNFQRKNGSLFNSPSTTAAAFMNLNNDSCLDYLHSLVQKFGNAVPTIYPLDIYAQLCMV 243
VM +QRKNGSLF+SPSTTA AFM+ N+D C +YL S++QKF ++VP IYPLDIYA+L MV
Sbjct: 217 VMQYQRKNGSLFSSPSTTAVAFMHRNDDGCFNYLRSILQKFHSSVPAIYPLDIYARLHMV 276
Query: 244 DNLQTLGIDRHFKEEIGSLLDKTYRHWLKGDENILLDASTCAMAFRMLH---------QL 303
D+LQ LGIDRHFK+EI S+LD+TYR W++G+ENI LDASTCAMAFRML QL
Sbjct: 277 DSLQKLGIDRHFKDEIRSVLDETYRCWMQGEENIFLDASTCAMAFRMLRVEGYDVSSDQL 336
Query: 304 AQFSEDRFSDCLGGYLKDIGSILELFKASQITICPEESVLDNINTWTN------------ 363
QFSED F +CLGG+LKD G+ LELFKASQI I P+E +L+NIN+WT+
Sbjct: 337 TQFSEDLFPNCLGGHLKDFGASLELFKASQIIIYPDEFILENINSWTSRFLNHGLSGGSV 396
Query: 364 ------------------------LNRIFRKLS-----------TLVNFSCLNLGHQDFL 423
L R+ K + + ++CLN GHQDFL
Sbjct: 397 HSDRTERLVKQEAVNAFEFPYNSTLERLSNKRALESYSGDIVRISKTTYACLNFGHQDFL 456
Query: 424 RLAMEEFYGCQCIQRQEKKDLERWVIENKLEKL-LPRHRVVYCYFTGAALITSPELTEAR 483
LA+E+F QCI R+E K+LE+WVIENKL+KL R ++ YCYF+ AA +TSPEL +AR
Sbjct: 457 ELAVEDFNTLQCIHRKELKELEKWVIENKLDKLKFARQKLAYCYFSAAATLTSPELCDAR 516
Query: 484 MSFGKSGVLTTVLDAFFDDWGSQDERTNLVQAAERWDVSNEMEFCSEEVEIIFKALRRAV 543
+S+ K+GVLTTV+D FFD GS++E NL+Q E+WD S E +CS+EVEIIF AL +
Sbjct: 517 LSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVEKWDASGEAGYCSKEVEIIFLALHSTI 576
Query: 544 DEISNAALTWQGRSVRSHVIDTWVELIKSMAMEADWSSNKTVPSMDEYIETSAVSFALGP 603
EI AL WQGRSV +VID W+ L++SM EA+W NK VPS+DEY+E VSFALGP
Sbjct: 577 CEIGEKALPWQGRSVTRNVIDIWLALLESMRKEAEWLKNKVVPSLDEYMENGYVSFALGP 636
Query: 604 IVLPALYFIGPKISEEMVGSFEYHNLFMLMSIAGRLLNDIKTIEKEAGEGKHNAFSLRML 663
IVLP LYF+GPK+ EE++G+ EY LF LMS +GRLLND +T ++E+ EGK NA SL ++
Sbjct: 637 IVLPTLYFVGPKLPEEIIGNCEYQKLFKLMSTSGRLLNDTRTFDRESSEGKLNALSLYLI 696
Query: 664 DGGVHITKEEAIRAIQVDIDRATRKLLRLVLQVEDSTIPRVCKDLFWRVNCAVHVFYRKD 690
G ++KEEA A++ D+DR R+LLRLVLQ E+STIPR CKDLFW+++C VH+FYRKD
Sbjct: 697 SAGGKLSKEEATEAMKGDVDRTRRELLRLVLQ-ENSTIPRACKDLFWKMSCVVHLFYRKD 756
BLAST of Spg021407 vs. ExPASy TrEMBL
Match:
A0A5A7V5B3 (Ent-kaur-16-ene synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold357G00260 PE=4 SV=1)
HSP 1 Score: 880.9 bits (2275), Expect = 3.4e-252
Identity = 439/745 (58.93%), Postives = 552/745 (74.09%), Query Frame = 0
Query: 4 FEGTKERIKKMFEKVELSVSAYDTAWMAMVPTPNCLKTPLFPECLNWLLDNQLPDGSWGL 63
FE TKERI+K+F+ VELSVSAYDTAW+AMVP+PN L PLFPEC+NW+LD+Q PDGSWG+
Sbjct: 111 FEDTKERIQKLFKNVELSVSAYDTAWVAMVPSPNSLNRPLFPECINWVLDHQNPDGSWGI 170
Query: 64 LHNYPILTKATLLSTFASILALKRWDVGQEHMIKGLQFIESNLDSATDEKQHSPIGFDII 123
LH++ ++ KATLLST A +L LKRW++G +HM K L FI+SN+ SATDE Q SP+GFDII
Sbjct: 171 LHDHQLVMKATLLSTLACVLTLKRWNIGHDHMSKALSFIKSNIASATDENQRSPVGFDII 230
Query: 124 FSGMIEYAKTLDLSLPLPQSNIDALIRRRELELSR--SNSEGKKAYLAYVSEGLGKSQDW 183
F GMIEYAK L+L+LPL N+DAL++++ELEL SNSEG KAYLAYVSEG+GK QDW
Sbjct: 231 FPGMIEYAKDLNLNLPLASMNVDALVQKKELELRSCCSNSEGGKAYLAYVSEGIGKLQDW 290
Query: 184 GMVMNFQRKNGSLFNSPSTTAAAFMNLNNDSCLDYLHSLVQKFGNAVPTIYPLDIYAQLC 243
MVM +QRKNGSLF+SPSTTA AFM+ N+D C +YL S++QKF ++VP IYPLDIYA+L
Sbjct: 291 DMVMQYQRKNGSLFSSPSTTAVAFMHRNDDGCFNYLRSILQKFHSSVPAIYPLDIYARLH 350
Query: 244 MVDNLQTLGIDRHFKEEIGSLLDKTYRHWLKGDENILLDASTCAMAFRMLH--------- 303
MVD+LQ LGIDRHFK+EI S+LD+TYR W++G+ENI LDASTCAMAFRML
Sbjct: 351 MVDSLQKLGIDRHFKDEIRSVLDETYRCWMQGEENIFLDASTCAMAFRMLRVEGYDVSSD 410
Query: 304 QLAQFSEDRFSDCLGGYLKDIGSILELFKASQITICPEESVLDNINTWTN---------- 363
QL QFSED F +CLGG+LKD G+ LELFKASQI I P+E +L+NIN+WT+
Sbjct: 411 QLTQFSEDLFPNCLGGHLKDFGASLELFKASQIIIYPDEFILENINSWTSRFLNHGLSGG 470
Query: 364 --------------------------LNRIFRKLS-----------TLVNFSCLNLGHQD 423
L R+ K + + ++CLN GHQD
Sbjct: 471 SVHFDRTERLVKQEAVNAFEFPYNSTLERLSNKRALESYSGDIVRISKTTYACLNFGHQD 530
Query: 424 FLRLAMEEFYGCQCIQRQEKKDLERWVIENKLEKL-LPRHRVVYCYFTGAALITSPELTE 483
FL LA+E+F QCI R+E K+LE+WVIENKL+KL R ++ YCYF+ AA +TSPEL +
Sbjct: 531 FLELAVEDFNTLQCIHRKELKELEKWVIENKLDKLKFARQKLAYCYFSAAATLTSPELCD 590
Query: 484 ARMSFGKSGVLTTVLDAFFDDWGSQDERTNLVQAAERWDVSNEMEFCSEEVEIIFKALRR 543
AR+S+ K+GVLTTV+D FFD GS++E NL+Q E+WD S E +CS+EVEIIF AL
Sbjct: 591 ARLSWAKNGVLTTVVDDFFDVGGSEEELVNLIQLVEKWDASGEAGYCSKEVEIIFLALHS 650
Query: 544 AVDEISNAALTWQGRSVRSHVIDTWVELIKSMAMEADWSSNKTVPSMDEYIETSAVSFAL 603
+ EI AL WQGRSV +VID W+ L++SM EA+W NK VPS+DEY+E VSFAL
Sbjct: 651 TICEIGEKALPWQGRSVTRNVIDIWLALLESMRKEAEWLKNKVVPSLDEYMENGYVSFAL 710
Query: 604 GPIVLPALYFIGPKISEEMVGSFEYHNLFMLMSIAGRLLNDIKTIEKEAGEGKHNAFSLR 663
GPIVLP LYF+GPK+ EE++G+ EY LF LMS +GRLLND +T ++E+ EGK NA SL
Sbjct: 711 GPIVLPTLYFVGPKLPEEIIGNCEYQKLFKLMSTSGRLLNDTRTFDRESSEGKLNALSLY 770
Query: 664 MLDGGVHITKEEAIRAIQVDIDRATRKLLRLVLQVEDSTIPRVCKDLFWRVNCAVHVFYR 690
++ G ++KEEA A++ D+DR R+LLRLVLQ E+STIPR CKDLFW+++C VH+FYR
Sbjct: 771 LISAGGKLSKEEATEAMKGDVDRTRRELLRLVLQ-ENSTIPRACKDLFWKMSCVVHLFYR 830
BLAST of Spg021407 vs. TAIR 10
Match:
AT1G79460.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )
HSP 1 Score: 663.3 bits (1710), Expect = 2.1e-190
Identity = 348/746 (46.65%), Postives = 487/746 (65.28%), Query Frame = 0
Query: 4 FEGTKERIKKMFEKVELSVSAYDTAWMAMVPTPNCLKTPLFPECLNWLLDNQLPDGSWGL 63
FE TKE+I+KM EKVELSVSAYDT+W+AMVP+P+ PLFP+C+ WLLDNQ DGSWGL
Sbjct: 35 FEQTKEKIRKMLEKVELSVSAYDTSWVAMVPSPSSQNAPLFPQCVKWLLDNQHEDGSWGL 94
Query: 64 -LHNYPILTKATLLSTFASILALKRWDVGQEHMIKGLQFIESNLDSATDEKQHSPIGFDI 123
H++ L K L ST ASILALK+W +G+ + KGLQFIE N TDE P GFDI
Sbjct: 95 DNHDHQSLKKDVLSSTLASILALKKWGIGERQINKGLQFIELNSALVTDETIQKPTGFDI 154
Query: 124 IFSGMIEYAKTLDLSLPLPQSNIDALIRRRELEL---SRSNSEGKKAYLAYVSEGLGKSQ 183
IF GMI+YA+ L+L++PL +D +IR+R+L+L S S+G++AYLAYV EG +
Sbjct: 155 IFPGMIKYARDLNLTIPLGSEVVDDMIRKRDLDLKCDSEKFSKGREAYLAYVLEGTRNLK 214
Query: 184 DWGMVMNFQRKNGSLFNSPSTTAAAFMNLNNDSCLDYLHSLVQKFGNAVPTIYPLDIYAQ 243
DW +++ +QRKNGSLF+SP+TTAAAF ND CL YL SL+QKF AVP++YP D YA+
Sbjct: 215 DWDLIVKYQRKNGSLFDSPATTAAAFTQFGNDGCLRYLCSLLQKFEAAVPSVYPFDQYAR 274
Query: 244 LCMVDNLQTLGIDRHFKEEIGSLLDKTYRHWLKGDENILLDASTCAMAFRML-------- 303
L ++ L++LGIDR FK EI S+LD+TYR+WL+GDE I LD +TCA+AFR+L
Sbjct: 275 LSIIVTLESLGIDRDFKTEIKSILDETYRYWLRGDEEICLDLATCALAFRLLLAHGYDVS 334
Query: 304 -HQLAQFSEDR-FSDCLGGYLKDIGSILELFKASQITICPEESVLDNINTWT-------- 363
L F+E+ FSD L GY+K+ S+LELFKA+Q P ES L WT
Sbjct: 335 YDPLKPFAEESGFSDTLEGYVKNTFSVLELFKAAQ--SYPHESALKKQCCWTKQYLEMEL 394
Query: 364 ---------------------------------------NLNRIFRKLSTLVNFSCLNLG 423
N + + T ++ N+
Sbjct: 395 SSWVKTSVRDKYLKKEVEDALAFPSYASLERSDHRRKILNGSAVENTRVTKTSYRLHNIC 454
Query: 424 HQDFLRLAMEEFYGCQCIQRQEKKDLERWVIENKLEKL-LPRHRVVYCYFTGAALITSPE 483
D L+LA+++F CQ I R+E + L+RW++EN+L++L R ++ YCYF+GAA + SPE
Sbjct: 455 TSDILKLAVDDFNFCQSIHREEMERLDRWIVENRLQELKFARQKLAYCYFSGAATLFSPE 514
Query: 484 LTEARMSFGKSGVLTTVLDAFFDDWGSQDERTNLVQAAERWDVSNEMEFCSEEVEIIFKA 543
L++AR+S+ K GVLTTV+D FFD GS++E NL+ E+WD++ E+ SE VEIIF
Sbjct: 515 LSDARISWAKGGVLTTVVDDFFDVGGSKEELENLIHLVEKWDLNGVPEYSSEHVEIIFSV 574
Query: 544 LRRAVDEISNAALTWQGRSVRSHVIDTWVELIKSMAMEADWSSNKTVPSMDEYIETSAVS 603
LR + E + A T+QGR+V H++ W++L+KSM EA+WSS+K+ PS+++Y+E + +S
Sbjct: 575 LRDTILETGDKAFTYQGRNVTHHIVKIWLDLLKSMLREAEWSSDKSTPSLEDYMENAYIS 634
Query: 604 FALGPIVLPALYFIGPKISEEMVGSFEYHNLFMLMSIAGRLLNDIKTIEKEAGEGKHNAF 663
FALGPIVLPA Y IGP + E+ V S +Y+ L+ L+S GRLLNDI+ ++E+ EGK NA
Sbjct: 635 FALGPIVLPATYLIGPPLPEKTVDSHQYNQLYKLVSTMGRLLNDIQGFKRESAEGKLNAV 694
Query: 664 SLRMLDGGVHITKEEAIRAIQVDIDRATRKLLRLVLQVEDSTIPRVCKDLFWRVNCAVHV 688
SL M + +KE I +++ +R +L +LVL+ + S +PR CK+ F +++ +++
Sbjct: 695 SLHMKHERDNRSKEVIIESMKGLAERKREELHKLVLEEKGSVVPRECKEAFLKMSKVLNL 754
BLAST of Spg021407 vs. TAIR 10
Match:
AT1G61120.1 (terpene synthase 04 )
HSP 1 Score: 230.3 bits (586), Expect = 4.6e-60
Identity = 222/760 (29.21%), Postives = 343/760 (45.13%), Query Frame = 0
Query: 20 LSVSAYDTAWMAMVP-----TPNCLKTPLFPECLNWLLDNQ-LPDGSWGLLHNYPILT-- 79
+S SAYDTAW++M+ N LK P+F CL+W++ NQ +G W +Y +
Sbjct: 36 VSPSAYDTAWLSMIEEDINVDDNELK-PMFQGCLDWIMCNQNAREGFWMNSTSYTTVADG 95
Query: 80 --------KATLLSTFASILALKRWDVGQEHMIKGLQFIESN----LDSATDEKQHSPIG 139
L ST A ++AL++W++G H+ KG ++IE N + +E+ P
Sbjct: 96 RDEDGEKDMCILTSTLACVVALQKWNIGCFHLHKGTRYIERNTEMIIGKYINEEGSYPRW 155
Query: 140 FDIIFSGMIEYAKTLDLSLPLPQSNIDAL---------IRRRELELSRSNSEGKKAYLAY 199
F I F+G++E A+ L L I+ + I +RE + N + AYL
Sbjct: 156 FAIKFTGILELAQKLGLHFVFSSRCIEMIKGMFYQRQEIIQREKLVHDCNYKPLLAYLEV 215
Query: 200 VSEGL-GKSQDWGMVMNFQRKNGSLFNSPSTTAAAFMNLNNDSCLDYLHSLVQKFGNAVP 259
+ L +Q+ +V + +GSLF SPS TA+AFM N CL YL +LVQK N VP
Sbjct: 216 LPSKLYVTNQEDIIVKSLDSMDGSLFQSPSATASAFMLTRNTKCLAYLQNLVQKCPNGVP 275
Query: 260 TIYPL-DIYAQLCMVDNLQTLGIDRHFKEEIGSLLDKTYRHWLKGD-----ENILLD-AS 319
YPL + +L MV+ +++ G+ F EI +L++ Y + + D + L D
Sbjct: 276 QKYPLNEDLIKLSMVNLIESTGLGEFFGIEIEHVLEQVYSRYEEKDFERMPMSYLADQLH 335
Query: 320 TCAMAFRMLHQLAQFSEDRF-------SDCLGGYLKDIGS----ILELFKASQITICPEE 379
++AFRML + R + ++I S IL +++A+ + E
Sbjct: 336 KDSLAFRMLRMHGRDVSPRSFCWFLNDQETRNHLERNIDSFLLVILSVYRATDLMFPGEH 395
Query: 380 SVLDNINTWTNL---------NRIFRKLSTL----------------VNFSCLNLGHQDF 439
+ + NL I +LST N + L++ F
Sbjct: 396 DLQEAREYTRNLLEKRRSIKEKMIMHELSTPWIARLKHLDHRMWIEDKNSNVLSMEKASF 455
Query: 440 LR-----------LAMEEFYGCQCIQRQEKKDLERWVIENKLEKL-LPRHRVVYCYFTGA 499
LR LA F Q +E ++L WV + L + R + YCYF
Sbjct: 456 LRLHSSYSDKLTHLAARNFEFQQAKYCRELEELTMWVKKWGLSDIGFGREKTTYCYF--- 515
Query: 500 ALITSPELTEARMSFG----KSGVLTTVLDAFFDDWGSQDERTNLVQAAERWDVSNEMEF 559
A +TS E + FG K+ +L T+ D FFD+ GS ++ L +A RW E E
Sbjct: 516 ATVTSLPY-EYAIKFGKLAAKTAILITIADDFFDEKGSFNDLEGLTKAVLRW----EGEE 575
Query: 560 CSEEVEIIFKALRRAVDEISNAALTWQGRSVRSHVIDTWVELIKSMAMEADWSSNKTVPS 619
IIF+AL V E +N T + H+ + W E +S EA+WS S
Sbjct: 576 LKSYGNIIFRALDDIVRETANTCRTHHKTDIIVHLRNIWGETFESWLREAEWSKKGHTSS 635
Query: 620 MDEYIETSAVSFALGPIVLPALYFIGPKISEEMVGSFEYHNLFMLMSIAGRLLNDIKTIE 679
MDEYI +S A I L + P + Y ++ L+ I RLLND+++ +
Sbjct: 636 MDEYIRNGMISIAAHTIALSISCLMEPCFPHNKLKPGNYDSITTLLMIIPRLLNDLQSYQ 695
Query: 680 KEAGEGKHNAFSLRMLD-GGVHITKEEAIRAIQVDIDRATRKLLRLVLQVEDSTIPRVCK 688
KE +GK N+ L M + G+ I E++I I+ ID ++ L VL S +P+ CK
Sbjct: 696 KEQEQGKMNSVLLHMKNHPGLEI--EDSIAHIEKIIDSKRKEFLEHVLVDGLSDLPKPCK 755
BLAST of Spg021407 vs. TAIR 10
Match:
AT4G02780.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )
HSP 1 Score: 221.5 bits (563), Expect = 2.1e-57
Identity = 150/507 (29.59%), Postives = 243/507 (47.93%), Query Frame = 0
Query: 19 ELSVSAYDTAWMAMVPTPNCLKTPLFPECLNWLLDNQLPDGSWGLLHNYPILTKATLLST 78
E+++SAYDTAW+A++ + KTP FP + W+ +NQL DGSWG Y L++T
Sbjct: 112 EITISAYDTAWVALIDAGD--KTPAFPSAVKWIAENQLSDGSWG--DAYLFSYHDRLINT 171
Query: 79 FASILALKRWDVGQEHMIKGLQFIESNLDSATDEK-QHSPIGFDIIFSGMIEYAKTLDLS 138
A ++AL+ W++ KG+ F N+ DE +H PIGF++ F ++E A+ +++
Sbjct: 172 LACVVALRSWNLFPHQCNKGITFFRENIGKLEDENDEHMPIGFEVAFPSLLEIARGINID 231
Query: 139 LPLPQSNIDALIRRRELELSRSNSE---GKKAYLAYVSEGLGKSQDWGMVMNFQRKNGSL 198
+P + + ++EL+L+R E L + EG+ + DW ++ Q ++GS
Sbjct: 232 VPYDSPVLKDIYAKKELKLTRIPKEIMHKIPTTLLHSLEGM-RDLDWEKLLKLQSQDGSF 291
Query: 199 FNSPSTTAAAFMNLNNDSCLDYLHSLVQKFGNAVPTIYPLDIYAQLCMVDNLQTLGIDRH 258
SPS+TA AFM + +CL+YL + V++F VP ++P+D++ + +VD LQ LGI R+
Sbjct: 292 LFSPSSTAFAFMQTRDSNCLEYLRNAVKRFNGGVPNVFPVDLFEHIWIVDRLQRLGISRY 351
Query: 259 FKEEIGSLLDKTYRHWLKGDENI-------LLDASTCAMAFRMLHQLA-QFSEDRFSD-- 318
F+EEI LD +R+W D I + D AMAFR+L Q Q S D F +
Sbjct: 352 FEEEIKECLDYVHRYWT--DNGICWARCSHVQDIDDTAMAFRLLRQHGYQVSADVFKNFE 411
Query: 319 ------CLGGYLKD-IGSILELFKASQITICPEESVLDN------------------INT 378
C G + + L++ASQ+ P E +L N I+
Sbjct: 412 KEGEFFCFVGQSNQAVTGMFNLYRASQLAF-PREEILKNAKEFSYNYLLEKREREELIDK 471
Query: 379 W-------------------TNLNRIFRKL--------------STLVNFSCLNLGHQDF 438
W +L R+ + TL +N + +
Sbjct: 472 WIIMKDLPGEIGFALEIPWYASLPRVETRFYIDQYGGENDVWIGKTLYRMPYVN--NNGY 531
Query: 439 LRLAMEEFYGCQCIQRQEKKDLERWVIENKLEKL-LPRHRVVYCYFTGAALITSPELTEA 453
L LA +++ CQ + E ++W EN+L + + R ++ CY+ AA I E +
Sbjct: 532 LELAKQDYNNCQAQHQLEWDIFQKWYEENRLSEWGVRRSELLECYYLAAATIFESERSHE 591
BLAST of Spg021407 vs. TAIR 10
Match:
AT1G61680.2 (terpene synthase 14 )
HSP 1 Score: 120.6 bits (301), Expect = 5.1e-27
Identity = 120/484 (24.79%), Postives = 219/484 (45.25%), Query Frame = 0
Query: 190 KNGSLFNSPSTTAAAFMNLNNDSCLDYLHSL-VQKFGNAVPTIYPLDIYAQ-LCMVDNLQ 249
K L N ++ + +N + L+YLH L ++K N + +D+ ++ L M+D +Q
Sbjct: 44 KRSILRNVHASVSNPSKQFHNKTSLEYLHELNIKKIKNILSA--NVDVPSENLEMIDVIQ 103
Query: 250 TLGIDRHFKEEIGSLLDKTYRHWLKGDENILLDASTCAMAFRMLHQLAQFSEDRFSDCLG 309
+LGID HF++EI L Y+ L+ + D A+ FR+L Q + ++
Sbjct: 104 SLGIDLHFRQEIEQTLHMIYKEGLQFNG----DLHEIALRFRLLRQEGHYVQENKKGGFK 163
Query: 310 GYLK-DIGSILELFKASQITICPEESVLDNIN--TWTNLNRI------------------ 369
+K D+ + ELF+AS++ + EE+ LD T++ LN +
Sbjct: 164 DVVKNDVKGLTELFEASELRVEGEET-LDGAREFTYSRLNELCSGRESHQKQEIMKSLAQ 223
Query: 370 -----FRKLSTLVNFSCLNLGHQD-------FLRLAMEEFYGCQCIQRQEKKDLERWVIE 429
R L++ S + + Q+ LR+A + + + + E +W E
Sbjct: 224 PRHKTVRGLTSKRFTSMIKIAGQEDPEWLQSLLRVAEIDSIRLKSLTQGEMSQTFKWWTE 283
Query: 430 NKLEKLL--PRHRVVYCYFTGAALITSPELTEARMSFGKSGVLTTVLDAFFDDWGSQDER 489
LEK + R + + + ++ P LTE R+ K L V+D FD +G +E
Sbjct: 284 LGLEKDVEKARSQPLKWHTWSMKILQDPTLTEQRLDLTKPISLVYVIDDIFDVYGELEEL 343
Query: 490 TNLVQAAERWDVSNEMEFCSEEVEIIFKALRRAVDEISNAALTWQGRSVRSHVIDTWVEL 549
T + ERWD ++ + + + F+AL EIS G + + +W L
Sbjct: 344 TIFTRVVERWD-HKGLKTLPKYMRVCFEALDMITTEISMKIYKSHGWNPTYALRQSWASL 403
Query: 550 IKSMAMEADWSSNKTVPSMDEYIETSAVSFALGPIVLPALYFIGPKISEEMVGSFEYHNL 609
K+ +EA W ++ +P+ +EY++ VS + ++L A +G ++++E V E N
Sbjct: 404 CKAFLVEAKWFNSGYLPTTEEYMKNGVVSSGVHLVMLHAYILLGEELTKEKVELIE-SNP 463
Query: 610 FMLMSIAG--RLLNDIKTIEKEAGEGKHNAFSLRMLDGGVHITKEEAIRAIQVDIDRATR 635
++ S A RL +D+ + + E +G ++ L+ T +EA + I RA +
Sbjct: 464 GIVSSAATILRLWDDLGSAKDENQDGTDGSYVECYLNEYKGSTVDEARTHVAQKISRAWK 518
BLAST of Spg021407 vs. TAIR 10
Match:
AT1G61680.1 (terpene synthase 14 )
HSP 1 Score: 120.2 bits (300), Expect = 6.7e-27
Identity = 122/491 (24.85%), Postives = 221/491 (45.01%), Query Frame = 0
Query: 190 KNGSLFNSPSTTAAAFMNLNNDSCLDYLHSL-VQKFGNAVPTIYPLDIYAQ-LCMVDNLQ 249
K L N ++ + +N + L+YLH L ++K N + +D+ ++ L M+D +Q
Sbjct: 44 KRSILRNVHASVSNPSKQFHNKTSLEYLHELNIKKIKNILSA--NVDVPSENLEMIDVIQ 103
Query: 250 TLGIDRHFKEEIGSLLDKTYRHWLKGDENILLDASTCAMAFRMLHQLAQFSED----RFS 309
+LGID HF++EI L Y+ L+ + D A+ FR+L Q + ++
Sbjct: 104 SLGIDLHFRQEIEQTLHMIYKEGLQFNG----DLHEIALRFRLLRQEGHYVQEIIFKNIL 163
Query: 310 DCLGGY----LKDIGSILELFKASQITICPEESVLDNIN--TWTNLNRI----------- 369
D GG+ D+ + ELF+AS++ + EE+ LD T++ LN +
Sbjct: 164 DKKGGFKDVVKNDVKGLTELFEASELRVEGEET-LDGAREFTYSRLNELCSGRESHQKQE 223
Query: 370 ------------FRKLSTLVNFSCLNLGHQD-------FLRLAMEEFYGCQCIQRQEKKD 429
R L++ S + + Q+ LR+A + + + + E
Sbjct: 224 IMKSLAQPRHKTVRGLTSKRFTSMIKIAGQEDPEWLQSLLRVAEIDSIRLKSLTQGEMSQ 283
Query: 430 LERWVIENKLEKLL--PRHRVVYCYFTGAALITSPELTEARMSFGKSGVLTTVLDAFFDD 489
+W E LEK + R + + + ++ P LTE R+ K L V+D FD
Sbjct: 284 TFKWWTELGLEKDVEKARSQPLKWHTWSMKILQDPTLTEQRLDLTKPISLVYVIDDIFDV 343
Query: 490 WGSQDERTNLVQAAERWDVSNEMEFCSEEVEIIFKALRRAVDEISNAALTWQGRSVRSHV 549
+G +E T + ERWD ++ + + + F+AL EIS G + +
Sbjct: 344 YGELEELTIFTRVVERWD-HKGLKTLPKYMRVCFEALDMITTEISMKIYKSHGWNPTYAL 403
Query: 550 IDTWVELIKSMAMEADWSSNKTVPSMDEYIETSAVSFALGPIVLPALYFIGPKISEEMVG 609
+W L K+ +EA W ++ +P+ +EY++ VS + ++L A +G ++++E V
Sbjct: 404 RQSWASLCKAFLVEAKWFNSGYLPTTEEYMKNGVVSSGVHLVMLHAYILLGEELTKEKVE 463
Query: 610 SFEYHNLFMLMSIAG--RLLNDIKTIEKEAGEGKHNAFSLRMLDGGVHITKEEAIRAIQV 635
E N ++ S A RL +D+ + + E +G ++ L+ T +EA +
Sbjct: 464 LIE-SNPGIVSSAATILRLWDDLGSAKDENQDGTDGSYVECYLNEYKGSTVDEARTHVAQ 523
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_022147909.1 | 5.1e-263 | 61.02 | ent-kaur-16-ene synthase, chloroplastic isoform X2 [Momordica charantia] | [more] |
| XP_022147908.1 | 1.7e-261 | 60.86 | ent-kaur-16-ene synthase, chloroplastic isoform X1 [Momordica charantia] | [more] |
| XP_022147910.1 | 1.7e-261 | 60.86 | ent-kaur-16-ene synthase, chloroplastic isoform X3 [Momordica charantia] | [more] |
| XP_016900364.1 | 8.2e-253 | 59.22 | PREDICTED: ent-kaur-16-ene synthase, chloroplastic isoform X2 [Cucumis melo] | [more] |
| KAA0062808.1 | 7.0e-252 | 58.93 | ent-kaur-16-ene synthase [Cucumis melo var. makuwa] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q39548 | 1.2e-246 | 58.12 | Ent-kaur-16-ene synthase, chloroplastic OS=Cucurbita maxima OX=3661 PE=1 SV=1 | [more] |
| A0A1Z3GC64 | 5.1e-197 | 48.00 | Ent-kaurene synthase 5, chloroplastic OS=Isodon rubescens OX=587669 GN=KSL5 PE=1... | [more] |
| A0A075FBT3 | 3.4e-193 | 47.67 | Ent-kaurene synthase, chloroplastic (Fragment) OS=Marrubium vulgare OX=41230 GN=... | [more] |
| A0A3G1QTS7 | 4.5e-193 | 47.47 | Ent-kaurene synthase 1, chloroplastic OS=Isodon eriocalyx OX=662907 GN=KS1 PE=1 ... | [more] |
| Q9XEH9 | 4.5e-193 | 47.42 | Ent-kaurene synthase 1, chloroplastic OS=Stevia rebaudiana OX=55670 GN=KS1-1 PE=... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1D2F0 | 2.5e-263 | 61.02 | ent-kaur-16-ene synthase, chloroplastic isoform X2 OS=Momordica charantia OX=367... | [more] |
| A0A6J1D2L1 | 8.0e-262 | 60.86 | ent-kaur-16-ene synthase, chloroplastic isoform X1 OS=Momordica charantia OX=367... | [more] |
| A0A6J1D1G1 | 8.0e-262 | 60.86 | ent-kaur-16-ene synthase, chloroplastic isoform X3 OS=Momordica charantia OX=367... | [more] |
| A0A1S4DWJ8 | 4.0e-253 | 59.22 | ent-kaur-16-ene synthase, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LO... | [more] |
| A0A5A7V5B3 | 3.4e-252 | 58.93 | Ent-kaur-16-ene synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol... | [more] |
