Spg021327 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg021327
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionAspartyl protease family protein 1-like
Locationscaffold6: 48051483 .. 48055438 (-)
RNA-Seq ExpressionSpg021327
SyntenySpg021327
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCCCTTCTTCCCTCTTCCTAACGCTCTGCGTTTTCTTTTCCATTTTCAGTTTCTTCTCCCACTCCTGCCACGCCCTCCAATCTTTCCGCTTCAAGATCCACCACCGCTACTCCGACGCCGTCCGTGGAATCCTCCCCGTCGATGGGTTACCCGAGGAAGGCACTTTCGACTACTACGCCGCCATGGTCCGCAGAGACCATTTTGTTCGTGCTCGTCGTCTTGCTGAAGATCGCCCTCCTCTCACTTTCCTCTCCGGCAACGAAACCATTCGACTCAACGCTCTGGGATTGTACGGTTTTCTGTTTGTTTATTCCCTCTTGAGTTGCTTTTGAGTTGAGATTTCTTTCTAACTATTGGGTCTTCTGTGTGAATTATGAACTTTTCACTTTCCTCTTTGAATCTCTGAATCTTCCTTTTGTGATTCTTATATTCACTAATTTTCCTTTTCCCCCTTCTTGTGTTGTTGGATTTCAGCCTGCATTACGCCGAGGTTACAGTGGGAACGCCGGGGGTTTCGTATTTGGTGGCACTGGACACTGGCAGCGATTTGTTCTGGTTACCATGTGATTGTGCTAATTGTTTGACTGGGCTTAACTCATCCCAAGGGGTAGCAATTCTTCAACCCTTTTCTTTCTAGCGCTCGATTTGATTTTTTTTGAGGGCCGGTTGATTCGTTCTTGAAGTTCTTGTCATGAGAAACACAAACACAAACACAATATGACATGGGCATGATGAATGATGTATGTGCTGTTTTCTCAGAAATTAGGACATAGACACTTTGTATACATGTTTGTTGAATTATGAATTTCTTTTAAGAACTAGGCGTGTTTTGTACATCTTTTGAGGACCTAAATCTCTGTTTGAAGGGATGTGGTGTCATATTAAAGAAGTGATAGAATATGTAACATTTATAAGATAGCAAAGAAGCCATTATATTATATAGTTGAATGGATGTGGTGTCATATAACAATCCGTGTCAAGTGGGCATTTATCCGAGTGGTTTAAGATCTTGTAAGATAAGGAGATTTAAAATCAAGCACATCCATTCATTACAATTATATTATATGTTAAAAGAAAAGTTTGATGTGTTTTTCTGTTCAAATTCCATTGGCTTTGTATATTATTATATCATTACTGATTTCCATAGAAATTCCCTCTATTACTGTTTCTGTCAACAGGTAGTAAAATTTAATATCTACAGCCCTAAAAATTCATCAACTAGCAAGGAGGTTCCATGTAGTAGTTCCTTGTGTTCACATGCAGACCAGTGCTTTCCACCAGGTGACAGGTGCCCTTATCATGTTTCATACCTGTCTGATAACACCTCTTCTACTGGCTACTTGGTAGAGGATATATTGCACTTAACCACAAACGATGGCCCATCAAAACCCGTTAAAGCAAGTATTACTCTGGGGTACGTTACTGTTTAATCTATTAAAAAATTTGGCTAGTTATTGCTGCAATCGATCTAATTGGTTTTGTTCTTCATGTATACTCTATTGGGTTATTGATATCTGGATATGTTGTCCTTTAGGTGTGGTAAGGACCAGAGTGGTGCATTTTTAGCCAATGCAGCACCAAATGGCTTATTTGGGCTAGGTATCGAGAATGTTTCAGTTCCTAGCATCTTGGCAAATCAAGGACTCACTTCAAATTCTTTTTCCTTATGTTTTGGAGCTGGTGGAATGGGAAGAATTGAATTTGGAGATAAGGGTAGTCCAGGTCAAAGTGAAACACCATTCAACTTGGGACATAAGCAGTAAGTGTCACTTCTTAATTGGTGTATATGTTAATTTCATGTTTTGTAAGCTGGAAAAATAGACATTTTCATCAAATCATTCTAGTGATCTTTCTTTTATTTTTGTTTCATTTTTTGTTCATATGCCAGTCCTACTTATAACATCAGCATCACTCAGATAAGTGTGGGAGGAAACGTTTCCAATCTTGGATTTTCTGCAGTTTTTGATTCTGGGACCTCATTTACCTACTTGAATGACCCGGCCTATTCACTTATTGCTGGCAAAGTAAGCACCCTACAAATTTATGTGCAGTTTCTTGTGCCATGTTCTTCAGTTTGCTTATCCATGTCATTCTGTAGTTTGCTTCTATGGTGGAAGAAAAGCCGTTTACGATGAATTCAGACCTCCCTTTTGAAAACTGCTATGAACTGAGGTAATGTGATATGGTTCTATATATCAGCAAAGCAAGAATTAAAGCTAATAAATAATAATAATCTCATTTGAGGAAAACTGGATCTCTTAATTTAGTTTCAGGAGAGATCAGGCTTATTAAAGTCAACTCAAAGAGTTGAATAAATAATTCTCTCTCTCTATATATATATATTTCAGTCGATTTTATAGTACAAGTTATAACATAATGTAAAAAGTATCCATGCTGAAAACATTTAGAATTATGACATCCAGAGCTAAAGATCCATAATAGTATACCCATTCAACAATTAACTTGACTAAGTCTCTAACCACAAATAATGACAGTGTTAAAATATTATAGCCAACTTTTAATATATATACAAGTTTGCAAACATTCAAATTCTAGAACATAAATTAACTAAGAAAATAATTACTACTTTCTATATTTTTTTTTACTATAACATAATTATACCAAGAGAAGATACCAGTTTAGAGACAAATTTCTCTTTTTCATAACCATGTGCTGACTTTCACTTTCTTTCAGCCCAAATCAAACCACCTTCACATACCCTGTGATGAATCTGACAATGAAAGGTGGTGCACATTTTTTCATCCATCGTCCAGTAGTTTTGGTAGCCTTGCAGGTAATGACTGCTGCAGTGTAATATTCTCATCGACTTGTCTTGCAGTTTGAGCTACTATTGTTAACTTTCTTTCTGATTATGATCTCCAGACGACACGTCTTTATTGTCTTGCCATTTCTAGAAGTGACAACATAAATATCATTGGACGTAAGTATCTCAGCTTTTAGCTGATAGTTTATTGACATCTTATTGAACTTGTCTTTTGTAGCATGGTCTTTCATTGTACTTTTGAGTTGCATATAGTTTCTTTTTTGTTTCATATCAAATGTCTATCTTTTAGACTTCGTGCTAATATCGAACCCCACACATGGAGGAGAGAAAAAATGGAGAATTTTGTTGATACACTAAGCCTAACATGTCGGATACACGTCTTTGAAGTTAAATCGACAATATGCGAATGGCAATCGCTTTGTTATATCTTTACATGGGTTTATACTAGACATGTTTGTATACTGCGATCAATCAAGGAACTTTTTTTTTTCTCATGATGTGTATCGATATTAACGCAGAAAACTTCATGACTGGTTATCACATTGTTTTTGACCGTGAAAAGATGGTTTTGGGATGGAAGGGATCGAACTGTGAGTATTTCTGCTGAAGTTCTCTTAGTTTCATTTCTCTTTTCGGGATAAAATTCGCGCTACCTTCTAACATGGGATCTTCATTATCTAGGCACTGGTTATGAGGATGAAAACATGAACAATCTTCCTGTCAGTCCATCGTCCGCCCCCGCTGCCGCCCCGGGCACAGCAATCAACCCACAAGCCAACAGCGAGATGAATAACACTTCTCAAACAATAGAAAAACCAAGACCTGCAAATAATAGTTCAAATCTTGTAAGATCAGTCATTCTCACGTTTTTAATGACTGTTGTTCCATTTTTGGTTTTTGTTTGATCATTTTGTTACTCATATTCTTGTATTGTTTAACACTTTTCAGTATAAGTTTATAGATGATTAATACATGGTTTCATATAGGTGAAATTGTTGTAAGTGAAAGTATAGAATGATTTATAACAATAAATTTATAGTGTGTATTGAGTCATGGACCTTGACCCTTCTTTCATTTCAACTTTGATGTTCTCTTCTCAACTCTGAGATGAAATGAGATTTGCAGATATTGAAGTTTATTTTGTTTTTATTTTTTTTATACATA

mRNA sequence

ATGGCTTCCCCTTCTTCCCTCTTCCTAACGCTCTGCGTTTTCTTTTCCATTTTCAGTTTCTTCTCCCACTCCTGCCACGCCCTCCAATCTTTCCGCTTCAAGATCCACCACCGCTACTCCGACGCCGTCCGTGGAATCCTCCCCGTCGATGGGTTACCCGAGGAAGGCACTTTCGACTACTACGCCGCCATGGTCCGCAGAGACCATTTTGTTCGTGCTCGTCGTCTTGCTGAAGATCGCCCTCCTCTCACTTTCCTCTCCGGCAACGAAACCATTCGACTCAACGCTCTGGGATTGTACGGTTTTCTGTTTCCTGCATTACGCCGAGGTTACAGTGGGAACGCCGGGGGTTTCGTATTTGGTGGCACTGGACACTGGCAGCGATTTGTTCTGGTAGTAAAATTTAATATCTACAGCCCTAAAAATTCATCAACTAGCAAGGAGGTTCCATGTAGTAGTTCCTTGTGTTCACATGCAGACCAGTGCTTTCCACCAGGTGACAGGTGCCCTTATCATGTTTCATACCTGTCTGATAACACCTCTTCTACTGGCTACTTGGTAGAGGATATATTGCACTTAACCACAAACGATGGCCCATCAAAACCCGTTAAAGCAAGTATTACTCTGGGGTGTGGTAAGGACCAGAGTGGTGCATTTTTAGCCAATGCAGCACCAAATGGCTTATTTGGGCTAGGTATCGAGAATGTTTCAGTTCCTAGCATCTTGGCAAATCAAGGACTCACTTCAAATTCTTTTTCCTTATGTTTTGGAGCTGGTGGAATGGGAAGAATTGAATTTGGAGATAAGGGTAGTCCAGGTCAAAGTGAAACACCATTCAACTTGGGACATAAGCATCCTACTTATAACATCAGCATCACTCAGATAAGTGTGGGAGGAAACGTTTCCAATCTTGGATTTTCTGCAGTTTTTGATTCTGGGACCTCATTTACCTACTTGAATGACCCGGCCTATTCACTTATTGCTGGCAAATTTGCTTCTATGGTGGAAGAAAAGCCGTTTACGATGAATTCAGACCTCCCTTTTGAAAACTGCTATGAACTGAGCCCAAATCAAACCACCTTCACATACCCTGTGATGAATCTGACAATGAAAGGTGGTGCACATTTTTTCATCCATCGTCCAGTAGTTTTGGTAGCCTTGCAGACGACACGTCTTTATTGTCTTGCCATTTCTAGAAGTGACAACATAAATATCATTGGACAAAACTTCATGACTGGTTATCACATTGTTTTTGACCGTGAAAAGATGGTTTTGGGATGGAAGGGATCGAACTGCACTGGTTATGAGGATGAAAACATGAACAATCTTCCTGTCAGTCCATCGTCCGCCCCCGCTGCCGCCCCGGGCACAGCAATCAACCCACAAGCCAACAGCGAGATGAATAACACTTCTCAAACAATAGAAAAACCAAGACCTGCAAATAATAGTTCAAATCTTGTAAGATCAGTCATTCTCACGTTTTTAATGACTGTTGTTCCATTTTTGGTTTTTGTTTGA

Coding sequence (CDS)

ATGGCTTCCCCTTCTTCCCTCTTCCTAACGCTCTGCGTTTTCTTTTCCATTTTCAGTTTCTTCTCCCACTCCTGCCACGCCCTCCAATCTTTCCGCTTCAAGATCCACCACCGCTACTCCGACGCCGTCCGTGGAATCCTCCCCGTCGATGGGTTACCCGAGGAAGGCACTTTCGACTACTACGCCGCCATGGTCCGCAGAGACCATTTTGTTCGTGCTCGTCGTCTTGCTGAAGATCGCCCTCCTCTCACTTTCCTCTCCGGCAACGAAACCATTCGACTCAACGCTCTGGGATTGTACGGTTTTCTGTTTCCTGCATTACGCCGAGGTTACAGTGGGAACGCCGGGGGTTTCGTATTTGGTGGCACTGGACACTGGCAGCGATTTGTTCTGGTAGTAAAATTTAATATCTACAGCCCTAAAAATTCATCAACTAGCAAGGAGGTTCCATGTAGTAGTTCCTTGTGTTCACATGCAGACCAGTGCTTTCCACCAGGTGACAGGTGCCCTTATCATGTTTCATACCTGTCTGATAACACCTCTTCTACTGGCTACTTGGTAGAGGATATATTGCACTTAACCACAAACGATGGCCCATCAAAACCCGTTAAAGCAAGTATTACTCTGGGGTGTGGTAAGGACCAGAGTGGTGCATTTTTAGCCAATGCAGCACCAAATGGCTTATTTGGGCTAGGTATCGAGAATGTTTCAGTTCCTAGCATCTTGGCAAATCAAGGACTCACTTCAAATTCTTTTTCCTTATGTTTTGGAGCTGGTGGAATGGGAAGAATTGAATTTGGAGATAAGGGTAGTCCAGGTCAAAGTGAAACACCATTCAACTTGGGACATAAGCATCCTACTTATAACATCAGCATCACTCAGATAAGTGTGGGAGGAAACGTTTCCAATCTTGGATTTTCTGCAGTTTTTGATTCTGGGACCTCATTTACCTACTTGAATGACCCGGCCTATTCACTTATTGCTGGCAAATTTGCTTCTATGGTGGAAGAAAAGCCGTTTACGATGAATTCAGACCTCCCTTTTGAAAACTGCTATGAACTGAGCCCAAATCAAACCACCTTCACATACCCTGTGATGAATCTGACAATGAAAGGTGGTGCACATTTTTTCATCCATCGTCCAGTAGTTTTGGTAGCCTTGCAGACGACACGTCTTTATTGTCTTGCCATTTCTAGAAGTGACAACATAAATATCATTGGACAAAACTTCATGACTGGTTATCACATTGTTTTTGACCGTGAAAAGATGGTTTTGGGATGGAAGGGATCGAACTGCACTGGTTATGAGGATGAAAACATGAACAATCTTCCTGTCAGTCCATCGTCCGCCCCCGCTGCCGCCCCGGGCACAGCAATCAACCCACAAGCCAACAGCGAGATGAATAACACTTCTCAAACAATAGAAAAACCAAGACCTGCAAATAATAGTTCAAATCTTGTAAGATCAGTCATTCTCACGTTTTTAATGACTGTTGTTCCATTTTTGGTTTTTGTTTGA

Protein sequence

MASPSSLFLTLCVFFSIFSFFSHSCHALQSFRFKIHHRYSDAVRGILPVDGLPEEGTFDYYAAMVRRDHFVRARRLAEDRPPLTFLSGNETIRLNALGLYGFLFPALRRGYSGNAGGFVFGGTGHWQRFVLVVKFNIYSPKNSSTSKEVPCSSSLCSHADQCFPPGDRCPYHVSYLSDNTSSTGYLVEDILHLTTNDGPSKPVKASITLGCGKDQSGAFLANAAPNGLFGLGIENVSVPSILANQGLTSNSFSLCFGAGGMGRIEFGDKGSPGQSETPFNLGHKHPTYNISITQISVGGNVSNLGFSAVFDSGTSFTYLNDPAYSLIAGKFASMVEEKPFTMNSDLPFENCYELSPNQTTFTYPVMNLTMKGGAHFFIHRPVVLVALQTTRLYCLAISRSDNINIIGQNFMTGYHIVFDREKMVLGWKGSNCTGYEDENMNNLPVSPSSAPAAAPGTAINPQANSEMNNTSQTIEKPRPANNSSNLVRSVILTFLMTVVPFLVFV
Homology
BLAST of Spg021327 vs. NCBI nr
Match: XP_038906112.1 (aspartyl protease family protein 1 [Benincasa hispida])

HSP 1 Score: 742.3 bits (1915), Expect = 2.8e-210
Identity = 385/521 (73.90%), Postives = 423/521 (81.19%), Query Frame = 0

Query: 1   MASPSSLFLTLCVFFSIFSFFSHSCHALQSFRFKIHHRYSDAVRGILPVDGLPEEGTFDY 60
           MASPS+ FLTLC FFSIF+F SHS HAL SF F IHH YSDAVR ILP+D LP+EGT DY
Sbjct: 1   MASPSTFFLTLCFFFSIFTFISHSSHALGSFTFNIHHLYSDAVRQILPLDALPQEGTLDY 60

Query: 61  YAAMVRRDHFVRARRLAEDRPPLTFLSGNETIRLNALGLYGFLFPALRRGYSGNAGGFVF 120
           YAAMVR DHFV +RRL +D+PPLTF SGN+T+R+N L   GFL+ A     +  A   V 
Sbjct: 61  YAAMVRTDHFVHSRRLVQDQPPLTFFSGNQTLRINPL---GFLYYAEVTVGTPEASYLVA 120

Query: 121 GGTG---HWQRFVLV------------VKFNIYSPKNSSTSKEVPCSSSLCSHADQCFPP 180
             TG    W     V            V FNIYSP NSSTSKEV CSSSLC+HADQC   
Sbjct: 121 LDTGSDLFWLPCDCVNCITGFNTSQGPVNFNIYSPSNSSTSKEVQCSSSLCAHADQCSSR 180

Query: 181 GDRCPYHVSYLSDNTSSTGYLVEDILHLTTNDGPSKPVKASITLGCGKDQSGAFLANAAP 240
            D CPY VSYLSDNTSSTGYLVEDILHLTTND  SKPV A ITLGCGKDQSGAFL++AAP
Sbjct: 181 SDTCPYEVSYLSDNTSSTGYLVEDILHLTTNDDQSKPVNAKITLGCGKDQSGAFLSSAAP 240

Query: 241 NGLFGLGIENVSVPSILANQGLTSNSFSLCFGAGGMGRIEFGDKGSPGQSETPFNLGHKH 300
           NGLFGLGIENVSVPSILAN GL SNSFSLCFG   MGRIEFGDKGSPGQSETPFNLG KH
Sbjct: 241 NGLFGLGIENVSVPSILANAGLISNSFSLCFGPARMGRIEFGDKGSPGQSETPFNLGRKH 300

Query: 301 PTYNISITQISVGGNVSNLGFSAVFDSGTSFTYLNDPAYSLIAGKFASMVEEKPFTMNSD 360
           PTYNISITQI VGG+VSN+  +A+FDSGTSFTYLNDPAYS+ A KF SM+EEK +TM+S 
Sbjct: 301 PTYNISITQIGVGGHVSNVDVAAIFDSGTSFTYLNDPAYSVFADKFDSMIEEKRYTMSSG 360

Query: 361 LPFENCYELSPNQTTFTYPVMNLTMKGGAHFFIHRPVVLVALQTTRLYCLAISRSDNINI 420
           LPFENCYELSPNQTTFTYPVMNLTMKGG HF I+ PVVL++ + T L+CLAI+RSD+INI
Sbjct: 361 LPFENCYELSPNQTTFTYPVMNLTMKGGGHFVINHPVVLISTRLTTLFCLAIARSDSINI 420

Query: 421 IGQNFMTGYHIVFDREKMVLGWKGSNCTGYEDENMNNLPVSPSSAPAAAP-GTAINPQAN 480
           IGQNFMTGYHIVFDREKMVLGWK SNCTGYEDEN NNLPV P+ APAAAP  T INPQAN
Sbjct: 421 IGQNFMTGYHIVFDREKMVLGWKESNCTGYEDENTNNLPVGPTPAPAAAPRSTIINPQAN 480

Query: 481 SEMNNTSQTIEKPRPANNSSNLVRSVILTFLMTVVPFLVFV 506
           S +NNTSQ+IEKP+P N+SSNL+ SVILTFLM+V PFL+FV
Sbjct: 481 SNINNTSQSIEKPKPTNSSSNLLTSVILTFLMSVGPFLLFV 518

BLAST of Spg021327 vs. NCBI nr
Match: XP_022971529.1 (aspartyl protease family protein 1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 737.3 bits (1902), Expect = 9.1e-209
Identity = 382/520 (73.46%), Postives = 418/520 (80.38%), Query Frame = 0

Query: 1   MASPSSLFLTLCVFFSIFSFFSHSCHALQSFRFKIHHRYSDAVRGILPVDGLPEEGTFDY 60
           MASPS   LTLCVFFS+FSF SHS  AL SF F IHHRYSD VRGILPVDGLPEEGT DY
Sbjct: 1   MASPSPFSLTLCVFFSVFSFLSHSSLALGSFSFDIHHRYSDVVRGILPVDGLPEEGTIDY 60

Query: 61  YAAMVRRDHFVRARRLAEDRPPLTFLSGNETIRLNALGLYGFL-----FPALRRGYSGNA 120
           YAAMVRRD  +  RRL+ED+PPLTFL GNETIR+N LG   +       P +    + + 
Sbjct: 61  YAAMVRRDILLHGRRLSEDQPPLTFLLGNETIRVNPLGFLNYAEVTVGTPKVSYLVALDT 120

Query: 121 GGFVF-------GGTGHWQRFVLVVKFNIYSPKNSSTSKEVPCSSSLCSHADQCFPPGDR 180
           G  +F            +  F    KFNIYSP NSSTSKEVPCSSSLC HA+QC  P D 
Sbjct: 121 GSDLFWLPCDCVNCVTEYNTFEWRAKFNIYSPSNSSTSKEVPCSSSLCQHANQCLSPSDP 180

Query: 181 CPYHVSYLSDNTSSTGYLVEDILHLTTNDGPSKPVKASITLGCGKDQSGAFLANAAPNGL 240
           CPY VSYLSDNTSSTGYLVEDILHL TNDG SKPV A+ITLGCG+DQSGAFL+ AAPNGL
Sbjct: 181 CPYKVSYLSDNTSSTGYLVEDILHLATNDGRSKPVNANITLGCGRDQSGAFLSTAAPNGL 240

Query: 241 FGLGIENVSVPSILANQGLTSNSFSLCFGAGGMGRIEFGDKGSPGQSETPFNLGHKHPTY 300
           FGLGIE+VSVPSILAN+GLTSNSFSLCFG  GMG IEFGDKGSPGQSETPFN+GH+HPTY
Sbjct: 241 FGLGIESVSVPSILANEGLTSNSFSLCFGPRGMGIIEFGDKGSPGQSETPFNVGHRHPTY 300

Query: 301 NISITQISVGGNVSNLGFSAVFDSGTSFTYLNDPAYSLIAGKFASMVEEKPFTMNSDLPF 360
           NISITQ++VGGNVSNL F+AVFDSGTSFTYLN+PAYSLIA KF SMV+EK +T N D+PF
Sbjct: 301 NISITQLNVGGNVSNLDFAAVFDSGTSFTYLNEPAYSLIADKFDSMVDEKRYTGNLDIPF 360

Query: 361 ENCYELSPNQTTFTYPVMNLTMKGGAHFFIHRPVVLVALQTTR-LYCLAISRSDNINIIG 420
           ENCYELSPNQT F YPVMNLTMKGGAHFFI+ P+V+ A + T  LYCLAISRSDNINIIG
Sbjct: 361 ENCYELSPNQTKFRYPVMNLTMKGGAHFFINHPIVVFASEATSWLYCLAISRSDNINIIG 420

Query: 421 QNFMTGYHIVFDREKMVLGWKGSNCTGYEDENMNNLPVSPSSAPAA--APGTAINPQANS 480
           QNFMTGYHIVFDREKMVLGWK SNCTGYED N NNLP+ PS+AP A  APGT I P+AN 
Sbjct: 421 QNFMTGYHIVFDREKMVLGWKESNCTGYEDVNTNNLPIHPSTAPTATPAPGTTIKPEANG 480

Query: 481 EMNNTSQTIEKPRPANNSSNLVRSVILTFLMTVVPFLVFV 506
           +MNNTSQT++KPR ANNS  L  SVIL  LM  VPFL FV
Sbjct: 481 QMNNTSQTLDKPRSANNSKKLGSSVILRLLMAGVPFLGFV 520

BLAST of Spg021327 vs. NCBI nr
Match: XP_023540295.1 (aspartyl protease family protein 1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 726.9 bits (1875), Expect = 1.2e-205
Identity = 374/520 (71.92%), Postives = 413/520 (79.42%), Query Frame = 0

Query: 1   MASPSSLFLTLCVFFSIFSFFSHSCHALQSFRFKIHHRYSDAVRGILPVDGLPEEGTFDY 60
           MASPS   LTLCVFFS+FSF S+S  AL SF F IHHRYSD VRGILPVDGLPEEGT DY
Sbjct: 1   MASPSPFSLTLCVFFSVFSFLSYSSLALGSFSFDIHHRYSDVVRGILPVDGLPEEGTVDY 60

Query: 61  YAAMVRRDHFVRARRLAEDRPPLTFLSGNETIRLNALGLYGFLFPALRRGYSGNAGGFVF 120
           YAAMVRRD  +  RRL+ED+PPLTFL GNET+R+N LG   +    +             
Sbjct: 61  YAAMVRRDILLHGRRLSEDQPPLTFLLGNETVRVNPLGFLHYAKVTVGTPKVSYLVALDT 120

Query: 121 GGTGHWQRFVLV------------VKFNIYSPKNSSTSKEVPCSSSLCSHADQCFPPGDR 180
           G    W     V             +FNIYSP NSSTSKEVPCSSSLC HA+QCF P D 
Sbjct: 121 GSDLFWLPCDCVNCVTEYNTSEGRARFNIYSPSNSSTSKEVPCSSSLCQHANQCFSPSDP 180

Query: 181 CPYHVSYLSDNTSSTGYLVEDILHLTTNDGPSKPVKASITLGCGKDQSGAFLANAAPNGL 240
           CPY VSYLSDNTSSTGYLVEDILHL TNDG SKPV A+ITLGCG+DQSGAFL+ AAPNGL
Sbjct: 181 CPYKVSYLSDNTSSTGYLVEDILHLATNDGRSKPVNANITLGCGRDQSGAFLSTAAPNGL 240

Query: 241 FGLGIENVSVPSILANQGLTSNSFSLCFGAGGMGRIEFGDKGSPGQSETPFNLGHKHPTY 300
           FGLGIE+VSVPSILAN+GLTSNSFSLCFG  GMGRIEFGDKGSPGQSETPFN+GH+HPTY
Sbjct: 241 FGLGIESVSVPSILANEGLTSNSFSLCFGPRGMGRIEFGDKGSPGQSETPFNVGHRHPTY 300

Query: 301 NISITQISVGGNVSNLGFSAVFDSGTSFTYLNDPAYSLIAGKFASMVEEKPFTMNSDLPF 360
           NISITQ++VGGNVSNL F+AVFDSGTSFTYLN+PAYSLIA KF SMV+EK +  N D+PF
Sbjct: 301 NISITQLNVGGNVSNLDFAAVFDSGTSFTYLNEPAYSLIADKFDSMVDEKRYMGNLDIPF 360

Query: 361 ENCYELSPNQTTFTYPVMNLTMKGGAHFFIHRPVVLVALQTTR-LYCLAISRSDNINIIG 420
           ENCYELSPNQT F YPVMNLTMKGGAHFFI+ P+V++A + T   YCLAISRSDNINIIG
Sbjct: 361 ENCYELSPNQTKFRYPVMNLTMKGGAHFFINHPIVVLASEATSWFYCLAISRSDNINIIG 420

Query: 421 QNFMTGYHIVFDREKMVLGWKGSNCTGYEDENMNNLPVSPSSAPAA--APGTAINPQANS 480
           QNFM GYHIVFDREKMVLGWK SNCTGYED   NNLP+ PS+AP A  APGT I P+ANS
Sbjct: 421 QNFMAGYHIVFDREKMVLGWKESNCTGYEDVKTNNLPIHPSTAPTAAPAPGTTIKPEANS 480

Query: 481 EMNNTSQTIEKPRPANNSSNLVRSVILTFLMTVVPFLVFV 506
           +MNN+S+T++KPR ANNS  L  +VIL  LM  VPFL FV
Sbjct: 481 QMNNSSETLDKPRSANNSEKLGSAVILRLLMAAVPFLCFV 520

BLAST of Spg021327 vs. NCBI nr
Match: XP_022943747.1 (aspartyl protease family protein 1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 724.2 bits (1868), Expect = 8.0e-205
Identity = 374/520 (71.92%), Postives = 412/520 (79.23%), Query Frame = 0

Query: 1   MASPSSLFLTLCVFFSIFSFFSHSCHALQSFRFKIHHRYSDAVRGILPVDGLPEEGTFDY 60
           MASPS   LTLCVFFS+FSF S S  AL SF F IHHRYSD VRGILPVDGLPEEGT DY
Sbjct: 1   MASPSPFSLTLCVFFSVFSFLSRSSLALGSFSFDIHHRYSDVVRGILPVDGLPEEGTVDY 60

Query: 61  YAAMVRRDHFVRARRLAEDRPPLTFLSGNETIRLNALGLYGFLFPALRRGYSGNAGGFVF 120
           Y AMVRRD  +  RRL+ED+PPLTFL GNET+R+N LG   +    +             
Sbjct: 61  YTAMVRRDILLHGRRLSEDQPPLTFLLGNETVRVNPLGFLHYAKVTVGTPKVSYLVALDT 120

Query: 121 GGTGHWQRFVLV------------VKFNIYSPKNSSTSKEVPCSSSLCSHADQCFPPGDR 180
           G    W     V             +FNIYSP NSSTSKEVPCSSSLC HA+QCF P D 
Sbjct: 121 GSDLFWLPCDCVNCVTEYNTSEGRARFNIYSPSNSSTSKEVPCSSSLCQHANQCFSPSDP 180

Query: 181 CPYHVSYLSDNTSSTGYLVEDILHLTTNDGPSKPVKASITLGCGKDQSGAFLANAAPNGL 240
           CPY VSYLSDNTSSTGYLVEDILHL TNDG SKPV A+ITLGCG+DQSGAFL+ AAPNGL
Sbjct: 181 CPYKVSYLSDNTSSTGYLVEDILHLATNDGRSKPVNANITLGCGRDQSGAFLSTAAPNGL 240

Query: 241 FGLGIENVSVPSILANQGLTSNSFSLCFGAGGMGRIEFGDKGSPGQSETPFNLGHKHPTY 300
           FGLGIE+VSVPSILAN+GLTSNSFSLCFG  GMGRIEFGDKGSPGQSETPFN+GH+HPTY
Sbjct: 241 FGLGIESVSVPSILANEGLTSNSFSLCFGPRGMGRIEFGDKGSPGQSETPFNVGHRHPTY 300

Query: 301 NISITQISVGGNVSNLGFSAVFDSGTSFTYLNDPAYSLIAGKFASMVEEKPFTMNSDLPF 360
           NISITQ++VGGNVSNL F+AVFDSGTSFTYLN+PAYSLIA KF SMV+EK +  N D+PF
Sbjct: 301 NISITQLNVGGNVSNLDFAAVFDSGTSFTYLNEPAYSLIADKFDSMVDEKRYMGNLDIPF 360

Query: 361 ENCYELSPNQTTFTYPVMNLTMKGGAHFFIHRPVVLVALQTTR-LYCLAISRSDNINIIG 420
           ENCYELSPNQT F YPVMNLTM+GGAHFFI+ P+V++A + T   YCLAISRSDNINIIG
Sbjct: 361 ENCYELSPNQTKFRYPVMNLTMEGGAHFFINHPIVVLASEATSWFYCLAISRSDNINIIG 420

Query: 421 QNFMTGYHIVFDREKMVLGWKGSNCTGYEDENMNNLPVSPSSAPAA--APGTAINPQANS 480
           QNFMTGYHIVFDREKMVLGWK SNCTGYED   NNLP+ PS+AP A  APGT I P+ANS
Sbjct: 421 QNFMTGYHIVFDREKMVLGWKESNCTGYEDVKTNNLPIHPSTAPTAAPAPGTTIKPEANS 480

Query: 481 EMNNTSQTIEKPRPANNSSNLVRSVILTFLMTVVPFLVFV 506
           +MNN+S+T++KPR ANNS  L  SVIL  LM  VPFL FV
Sbjct: 481 QMNNSSETLDKPRSANNSKKLGSSVILRLLMAGVPFLGFV 520

BLAST of Spg021327 vs. NCBI nr
Match: KAG6596257.1 (Aspartyl protease family protein 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 715.3 bits (1845), Expect = 3.7e-202
Identity = 366/508 (72.05%), Postives = 404/508 (79.53%), Query Frame = 0

Query: 1   MASPSSLFLTLCVFFSIFSFFSHSCHALQSFRFKIHHRYSDAVRGILPVDGLPEEGTFDY 60
           MASPS   LTLCVFFS+ SF S S  AL SF F IHHRYSD VRGILPVDGLPEEGT DY
Sbjct: 1   MASPSPFSLTLCVFFSVISFLSRSSLALGSFSFDIHHRYSDVVRGILPVDGLPEEGTVDY 60

Query: 61  YAAMVRRDHFVRARRLAEDRPPLTFLSGNETIRLNALGLYGFLFPALRRGYSGNAGGFVF 120
           Y AMVRRD  +  RRL+ED+PPLTFL GNET+R+N LG   +    +             
Sbjct: 61  YTAMVRRDILLHGRRLSEDQPPLTFLLGNETVRVNPLGFLHYAKVTVGTPKVSYLVALDT 120

Query: 121 GGTGHWQRFVLV------------VKFNIYSPKNSSTSKEVPCSSSLCSHADQCFPPGDR 180
           G    W     V             KFNIYSP NSSTSKEVPCSSSLC HA+QCF P D 
Sbjct: 121 GSDLFWLPCDCVNCVTEYNTSEGRAKFNIYSPSNSSTSKEVPCSSSLCQHANQCFSPSDP 180

Query: 181 CPYHVSYLSDNTSSTGYLVEDILHLTTNDGPSKPVKASITLGCGKDQSGAFLANAAPNGL 240
           CPY +SYLSDNTSSTGYLVEDILHL TNDG SKPV A+ITLGCG+DQSGAFL+ AAPNGL
Sbjct: 181 CPYKISYLSDNTSSTGYLVEDILHLATNDGRSKPVNANITLGCGRDQSGAFLSTAAPNGL 240

Query: 241 FGLGIENVSVPSILANQGLTSNSFSLCFGAGGMGRIEFGDKGSPGQSETPFNLGHKHPTY 300
           FGLGIE+VSVPSILAN+GLTSNSFSLCFG  GMGRIEFGDKGSPGQSETPFN+GH+HPTY
Sbjct: 241 FGLGIESVSVPSILANEGLTSNSFSLCFGPRGMGRIEFGDKGSPGQSETPFNVGHRHPTY 300

Query: 301 NISITQISVGGNVSNLGFSAVFDSGTSFTYLNDPAYSLIAGKFASMVEEKPFTMNSDLPF 360
           NISITQ++VGGNVSNL F+AVFDSGTSFTYLN+PAYSLIA KF SMV+EK +  N D+PF
Sbjct: 301 NISITQLNVGGNVSNLDFAAVFDSGTSFTYLNEPAYSLIADKFDSMVDEKRYMGNFDIPF 360

Query: 361 ENCYELSPNQTTFTYPVMNLTMKGGAHFFIHRPVVLVALQTTR-LYCLAISRSDNINIIG 420
           ENCYELSPNQT F YPVMNLTMKGGAHFFI+ P+V++A + T   YCLAISRSDNINIIG
Sbjct: 361 ENCYELSPNQTKFRYPVMNLTMKGGAHFFINHPIVVLASEATSWFYCLAISRSDNINIIG 420

Query: 421 QNFMTGYHIVFDREKMVLGWKGSNCTGYEDENMNNLPVSPSSAPAA--APGTAINPQANS 480
           QNFMTGYHIVFDREKMVLGWK SNCTGYED   NNLP+ PS+AP A  APGT I P+ANS
Sbjct: 421 QNFMTGYHIVFDREKMVLGWKESNCTGYEDVKTNNLPIHPSTAPTAAPAPGTTIKPEANS 480

Query: 481 EMNNTSQTIEKPRPANNSSNLVRSVILT 494
           +MNN+S+T++KPR ANNS  L  SVIL+
Sbjct: 481 QMNNSSETLDKPRSANNSKKLGSSVILS 508

BLAST of Spg021327 vs. ExPASy Swiss-Prot
Match: Q8VYV9 (Aspartyl protease family protein 1 OS=Arabidopsis thaliana OX=3702 GN=APF1 PE=2 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 2.1e-123
Identity = 239/486 (49.18%), Postives = 321/486 (66.05%), Query Frame = 0

Query: 7   LFLTLCVFFSIFSFFSHSCHALQSFRFKIHHRYSDAVRGILPVDGLPEEGTFDYYAAMVR 66
           LFL L +  +  S+    C     F F+ HHR+SD V G+LP DGLP   +  YY  M  
Sbjct: 10  LFLGLLILLA-SSWVLDRCEGFGEFGFEFHHRFSDQVVGVLPGDGLPNRDSSKYYRVMAH 69

Query: 67  RDHFVRARRLA-EDRPPLTFLSGNETIRLNALGLYGFL-----FPALRRGYSGNAGGFVF 126
           RD  +R RRLA ED+  +TF  GNET+R++ALG   +       P+     + + G  +F
Sbjct: 70  RDRLIRGRRLANEDQSLVTFSDGNETVRVDALGFLHYANVTVGTPSDWFMVALDTGSDLF 129

Query: 127 GGTGHWQRFVLVVK--------FNIYSPKNSSTSKEVPCSSSLCSHADQCFPPGDRCPYH 186
                    V  +K         NIYSP  SSTS +VPC+S+LC+  D+C  P   CPY 
Sbjct: 130 WLPCDCTNCVRELKAPGGSSLDLNIYSPNASSTSTKVPCNSTLCTRGDRCASPESDCPYQ 189

Query: 187 VSYLSDNTSSTGYLVEDILHLTTNDGPSKPVKASITLGCGKDQSGAFLANAAPNGLFGLG 246
           + YLS+ TSSTG LVED+LHL +ND  SK + A +T GCG+ Q+G F   AAPNGLFGLG
Sbjct: 190 IRYLSNGTSSTGVLVEDVLHLVSNDKSSKAIPARVTFGCGQVQTGVFHDGAAPNGLFGLG 249

Query: 247 IENVSVPSILANQGLTSNSFSLCFGAGGMGRIEFGDKGSPGQSETPFNLGHKHPTYNISI 306
           +E++SVPS+LA +G+ +NSFS+CFG  G GRI FGDKGS  Q ETP N+   HPTYNI++
Sbjct: 250 LEDISVPSVLAKEGIAANSFSMCFGNDGAGRISFGDKGSVDQRETPLNIRQPHPTYNITV 309

Query: 307 TQISVGGNVSNLGFSAVFDSGTSFTYLNDPAYSLIAGKFASMVEEKPF-TMNSDLPFENC 366
           T+ISVGGN  +L F AVFDSGTSFTYL D AY+LI+  F S+  +K + T +S+LPFE C
Sbjct: 310 TKISVGGNTGDLEFDAVFDSGTSFTYLTDAAYTLISESFNSLALDKRYQTTDSELPFEYC 369

Query: 367 YELSPNQTTFTYPVMNLTMKGGAHFFIHRPVVLVALQTTRLYCLAISRSDNINIIGQNFM 426
           Y LSPN+ +F YP +NLTMKGG+ + ++ P+V++ ++ T +YCLAI + ++I+IIGQNFM
Sbjct: 370 YALSPNKDSFQYPAVNLTMKGGSSYPVYHPLVVIPMKDTDVYCLAIMKIEDISIIGQNFM 429

Query: 427 TGYHIVFDREKMVLGWKGSNC-TGYEDENMNNLPVSPSSAPAAAPGTAINPQAN---SEM 474
           TGY +VFDREK++LGWK S+C TG  + +   LP + SS+ A  P ++ +P+A    S+ 
Sbjct: 430 TGYRVVFDREKLILGWKESDCYTG--ETSARTLPSNRSSSSARPPASSFDPEATNIPSQR 489

BLAST of Spg021327 vs. ExPASy Swiss-Prot
Match: Q9LX20 (Aspartic proteinase-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At5g10080 PE=2 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 4.9e-56
Identity = 170/492 (34.55%), Postives = 230/492 (46.75%), Query Frame = 0

Query: 6   SLFLTLCVFF-----SIFSFFSHSCHALQSFRFKIHHRYSDAVRGILPV----DGLPEEG 65
           S FL  CV F     ++ S FS           ++ HR+SD  R  +      D LP + 
Sbjct: 5   SAFLLFCVLFLATEETLASLFSS----------RLIHRFSDEGRASIKTPSSSDSLPNKQ 64

Query: 66  TFDYYAAMVRRDHFVRARRLAEDRPPLTFLSGNETIRL-NALGLYGFLF-----PALRRG 125
           + +YY  +   D   +   L      L    G++TI   N  G   + +     P++   
Sbjct: 65  SLEYYRLLAESDFRRQRMNLGAKVQSLVPSEGSKTISSGNDFGWLHYTWIDIGTPSVSFL 124

Query: 126 YSGNAGGFVF----------GGTGHWQRFVLVVKFNIYSPKNSSTSKEVPCSSSLCSHAD 185
            + + G  +             T  +   +     N Y+P +SSTSK   CS  LC  A 
Sbjct: 125 VALDTGSNLLWIPCNCVQCAPLTSTYYSSLATKDLNEYNPSSSSTSKVFLCSHKLCDSAS 184

Query: 186 QCFPPGDRCPYHVSYLSDNTSSTGYLVEDILHLTTND-----GPSKPVKASITLGCGKDQ 245
            C  P ++CPY V+YLS NTSS+G LVEDILHLT N        S  VKA + +GCGK Q
Sbjct: 185 DCESPKEQCPYTVNYLSGNTSSSGLLVEDILHLTYNTNNRLMNGSSSVKARVVIGCGKKQ 244

Query: 246 SGAFLANAAPNGLFGLGIENVSVPSILANQGLTSNSFSLCFGAGGMGRIEFGDKGSPGQS 305
           SG +L   AP+GL GLG   +SVPS L+  GL  NSFSLCF     GRI FGD G   Q 
Sbjct: 245 SGDYLDGVAPDGLMGLGPAEISVPSFLSKAGLMRNSFSLCFDEEDSGRIYFGDMGPSIQQ 304

Query: 306 ETPFNL--GHKHPTYNISITQISVGGN-VSNLGFSAVFDSGTSFTYLNDPAYSLIAGKFA 365
            TPF     +K+  Y + +    +G + +    F+   DSG SFTYL +  Y  +A +  
Sbjct: 305 STPFLQLDNNKYSGYIVGVEACCIGNSCLKQTSFTTFIDSGQSFTYLPEEIYRKVALEID 364

Query: 366 SMVEEKPFTMNSDLPFENCYELSPNQTTFTYPVMNLTMKGGAHFFIHRPV-VLVALQTTR 425
             +          + +E CYE S        P + L       F IH+P+ V    Q   
Sbjct: 365 RHINATSKNFEG-VSWEYCYESSAEPKV---PAIKLKFSHNNTFVIHKPLFVFQQSQGLV 424

Query: 426 LYCLAISRS--DNINIIGQNFMTGYHIVFDREKMVLGWKGSNCTGYEDENMNNLPVSPSS 462
            +CL IS S  + I  IGQN+M GY +VFDRE M LGW  S C   ED+      + P  
Sbjct: 425 QFCLPISPSGQEGIGSIGQNYMRGYRMVFDRENMKLGWSPSKC--QEDK------IEP-- 471

BLAST of Spg021327 vs. ExPASy Swiss-Prot
Match: Q4V3D2 (Aspartic proteinase 36 OS=Arabidopsis thaliana OX=3702 GN=A36 PE=1 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 7.9e-22
Identity = 101/366 (27.60%), Postives = 159/366 (43.44%), Query Frame = 0

Query: 131 LVVKFNIYSPKNSSTSKEVPCSSSLCSHADQCFPPGDR--CPYHVSYLSDNTSSTGYLVE 190
           L +  ++Y  K SSTSK V C    CS   Q    G +  C YHV Y   +TS   ++ +
Sbjct: 118 LGIPLSLYDSKTSSTSKNVGCEDDFCSFIMQSETCGAKKPCSYHVVYGDGSTSDGDFIKD 177

Query: 191 DI-LHLTTNDGPSKPVKASITLGCGKDQSGAF-LANAAPNGLFGLGIENVSVPSILANQG 250
           +I L   T +  + P+   +  GCGK+QSG     ++A +G+ G G  N S+ S LA  G
Sbjct: 178 NITLEQVTGNLRTAPLAQEVVFGCGKNQSGQLGQTDSAVDGIMGFGQSNTSIISQLAAGG 237

Query: 251 LTSNSFSLCF-GAGGMGRIEFGDKGSPGQSETPFNLGHKHPTYNISITQISVGGNVSNLG 310
            T   FS C     G G    G+  SP    TP      H  YN+ +  + V G+  +L 
Sbjct: 238 STKRIFSHCLDNMNGGGIFAVGEVESPVVKTTPIVPNQVH--YNVILKGMDVDGDPIDLP 297

Query: 311 FS---------AVFDSGTSFTYLNDPAYSLIAGKFASMVEEKPFTMNSDLPFENCYELSP 370
            S          + DSGT+  YL    Y+ +  K  +  + K   +        C+  + 
Sbjct: 298 PSLASTNGDGGTIIDSGTTLAYLPQNLYNSLIEKITAKQQVKLHMVQETFA---CFSFTS 357

Query: 371 NQTTFTYPVMNLTMKGGAHFFIHRPVVLVALQTTRLYCL-----AISRSDNINII--GQN 430
           N T   +PV+NL  +      ++    L +L+   +YC       ++  D  ++I  G  
Sbjct: 358 N-TDKAFPVVNLHFEDSLKLSVYPHDYLFSLRED-MYCFGWQSGGMTTQDGADVILLGDL 417

Query: 431 FMTGYHIVFDREKMVLGWKGSNCTGYEDENMNNLPVSPSSAPAAAPGTA-INPQANSEMN 475
            ++   +V+D E  V+GW   NC+       +++ V   S  A   G   +   A+S MN
Sbjct: 418 VLSNKLVVYDLENEVIGWADHNCS-------SSIKVKDGSGAAYQLGAENLISAASSVMN 469

BLAST of Spg021327 vs. ExPASy Swiss-Prot
Match: Q9M9A8 (Aspartyl protease APCB1 OS=Arabidopsis thaliana OX=3702 GN=APCB1 PE=1 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 1.3e-16
Identity = 85/332 (25.60%), Postives = 145/332 (43.67%), Query Frame = 0

Query: 137 IYSPK--NSSTSKEVPC----SSSLCSHADQCFPPGDRCPYHVSYLSDNTSSTGYLVEDI 196
           +Y P+  N   S E  C     + L  H + C     +C Y + Y +D++ S G L +D 
Sbjct: 247 LYKPRKDNLVRSSEAFCVEVQRNQLTEHCENC----HQCDYEIEY-ADHSYSMGVLTKDK 306

Query: 197 LHLTTNDGPSKPVKASITLGCGKDQSGAFLAN-AAPNGLFGLGIENVSVPSILANQGLTS 256
            HL  ++G     ++ I  GCG DQ G  L      +G+ GL    +S+PS LA++G+ S
Sbjct: 307 FHLKLHNG--SLAESDIVFGCGYDQQGLLLNTLLKTDGILGLSRAKISLPSQLASRGIIS 366

Query: 257 NSFSLCFGA--GGMGRIEFGDKGSP--GQSETPFNLGHKHPTYNISITQISVGGNVSNLG 316
           N    C  +   G G I  G    P  G +  P     +   Y + +T++S G  + +L 
Sbjct: 367 NVVGHCLASDLNGEGYIFMGSDLVPSHGMTWVPMLHDSRLDAYQMQVTKMSYGQGMLSLD 426

Query: 317 ------FSAVFDSGTSFTYLNDPAYSLIAGKFASMVEEKPFTMNSDLPFENCYELSPNQT 376
                    +FD+G+S+TY  + AYS +      +   +    +SD     C+     +T
Sbjct: 427 GENGRVGKVLFDTGSSYTYFPNQAYSQLVTSLQEVSGLELTRDDSDETLPICWRA---KT 486

Query: 377 TFTYPVMN--------LTMKGGAHFFI------HRPVVLVALQTTRLYCLAISRSDNIN- 433
            F +  ++        +T++ G+ + I       +P   + +      CL I    +++ 
Sbjct: 487 NFPFSSLSDVKKFFRPITLQIGSKWLIISRKLLIQPEDYLIISNKGNVCLGILDGSSVHD 546

BLAST of Spg021327 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 3.2e-15
Identity = 78/318 (24.53%), Postives = 133/318 (41.82%), Query Frame = 0

Query: 137 IYSPKNSSTSKEVPCSSSLCSHADQCFPPGDRCPYHVSYLSDNTSSTGYLVEDILHLTTN 196
           I++P++SS+   +PC S  C          + C Y   Y  D +++ GY+  +     T+
Sbjct: 137 IFNPQDSSSFSTLPCESQYCQDLPSETCNNNECQYTYGY-GDGSTTQGYMATETFTFETS 196

Query: 197 DGPSKPVKASITLGCGKDQSGAFLANAAPNGLFGLGIENVSVPSILANQGLTSNSFSLC- 256
             P      +I  GCG+D  G    N A  GL G+G   +S+PS L         FS C 
Sbjct: 197 SVP------NIAFGCGEDNQGFGQGNGA--GLIGMGWGPLSLPSQLG-----VGQFSYCM 256

Query: 257 --FGAGGMGRIEFGDKGS---PGQSETPFNLGHKHPT-YNISITQISVGGNVSNLGFSA- 316
             +G+     +  G   S    G   T       +PT Y I++  I+VGG+  NLG  + 
Sbjct: 257 TSYGSSSPSTLALGSAASGVPEGSPSTTLIHSSLNPTYYYITLQGITVGGD--NLGIPSS 316

Query: 317 ------------VFDSGTSFTYLNDPAYSLIAGKFASMVEEKPFTMNSDLPFENCYELSP 376
                       + DSGT+ TYL   AY+ +A  F   +   P    S      C++   
Sbjct: 317 TFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQI-NLPTVDESSSGLSTCFQQPS 376

Query: 377 NQTTFTYPVMNLTMKGGAHFFIHRPVVLVALQTTRLYCLAISRSD--NINIIGQNFMTGY 433
           + +T   P +++   GG      + +++   +   + CLA+  S    I+I G       
Sbjct: 377 DGSTVQVPEISMQFDGGVLNLGEQNILISPAE--GVICLAMGSSSQLGISIFGNIQQQET 435

BLAST of Spg021327 vs. ExPASy TrEMBL
Match: A0A6J1I8U2 (aspartyl protease family protein 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111470223 PE=3 SV=1)

HSP 1 Score: 737.3 bits (1902), Expect = 4.4e-209
Identity = 382/520 (73.46%), Postives = 418/520 (80.38%), Query Frame = 0

Query: 1   MASPSSLFLTLCVFFSIFSFFSHSCHALQSFRFKIHHRYSDAVRGILPVDGLPEEGTFDY 60
           MASPS   LTLCVFFS+FSF SHS  AL SF F IHHRYSD VRGILPVDGLPEEGT DY
Sbjct: 1   MASPSPFSLTLCVFFSVFSFLSHSSLALGSFSFDIHHRYSDVVRGILPVDGLPEEGTIDY 60

Query: 61  YAAMVRRDHFVRARRLAEDRPPLTFLSGNETIRLNALGLYGFL-----FPALRRGYSGNA 120
           YAAMVRRD  +  RRL+ED+PPLTFL GNETIR+N LG   +       P +    + + 
Sbjct: 61  YAAMVRRDILLHGRRLSEDQPPLTFLLGNETIRVNPLGFLNYAEVTVGTPKVSYLVALDT 120

Query: 121 GGFVF-------GGTGHWQRFVLVVKFNIYSPKNSSTSKEVPCSSSLCSHADQCFPPGDR 180
           G  +F            +  F    KFNIYSP NSSTSKEVPCSSSLC HA+QC  P D 
Sbjct: 121 GSDLFWLPCDCVNCVTEYNTFEWRAKFNIYSPSNSSTSKEVPCSSSLCQHANQCLSPSDP 180

Query: 181 CPYHVSYLSDNTSSTGYLVEDILHLTTNDGPSKPVKASITLGCGKDQSGAFLANAAPNGL 240
           CPY VSYLSDNTSSTGYLVEDILHL TNDG SKPV A+ITLGCG+DQSGAFL+ AAPNGL
Sbjct: 181 CPYKVSYLSDNTSSTGYLVEDILHLATNDGRSKPVNANITLGCGRDQSGAFLSTAAPNGL 240

Query: 241 FGLGIENVSVPSILANQGLTSNSFSLCFGAGGMGRIEFGDKGSPGQSETPFNLGHKHPTY 300
           FGLGIE+VSVPSILAN+GLTSNSFSLCFG  GMG IEFGDKGSPGQSETPFN+GH+HPTY
Sbjct: 241 FGLGIESVSVPSILANEGLTSNSFSLCFGPRGMGIIEFGDKGSPGQSETPFNVGHRHPTY 300

Query: 301 NISITQISVGGNVSNLGFSAVFDSGTSFTYLNDPAYSLIAGKFASMVEEKPFTMNSDLPF 360
           NISITQ++VGGNVSNL F+AVFDSGTSFTYLN+PAYSLIA KF SMV+EK +T N D+PF
Sbjct: 301 NISITQLNVGGNVSNLDFAAVFDSGTSFTYLNEPAYSLIADKFDSMVDEKRYTGNLDIPF 360

Query: 361 ENCYELSPNQTTFTYPVMNLTMKGGAHFFIHRPVVLVALQTTR-LYCLAISRSDNINIIG 420
           ENCYELSPNQT F YPVMNLTMKGGAHFFI+ P+V+ A + T  LYCLAISRSDNINIIG
Sbjct: 361 ENCYELSPNQTKFRYPVMNLTMKGGAHFFINHPIVVFASEATSWLYCLAISRSDNINIIG 420

Query: 421 QNFMTGYHIVFDREKMVLGWKGSNCTGYEDENMNNLPVSPSSAPAA--APGTAINPQANS 480
           QNFMTGYHIVFDREKMVLGWK SNCTGYED N NNLP+ PS+AP A  APGT I P+AN 
Sbjct: 421 QNFMTGYHIVFDREKMVLGWKESNCTGYEDVNTNNLPIHPSTAPTATPAPGTTIKPEANG 480

Query: 481 EMNNTSQTIEKPRPANNSSNLVRSVILTFLMTVVPFLVFV 506
           +MNNTSQT++KPR ANNS  L  SVIL  LM  VPFL FV
Sbjct: 481 QMNNTSQTLDKPRSANNSKKLGSSVILRLLMAGVPFLGFV 520

BLAST of Spg021327 vs. ExPASy TrEMBL
Match: A0A6J1FTX0 (aspartyl protease family protein 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448401 PE=3 SV=1)

HSP 1 Score: 724.2 bits (1868), Expect = 3.9e-205
Identity = 374/520 (71.92%), Postives = 412/520 (79.23%), Query Frame = 0

Query: 1   MASPSSLFLTLCVFFSIFSFFSHSCHALQSFRFKIHHRYSDAVRGILPVDGLPEEGTFDY 60
           MASPS   LTLCVFFS+FSF S S  AL SF F IHHRYSD VRGILPVDGLPEEGT DY
Sbjct: 1   MASPSPFSLTLCVFFSVFSFLSRSSLALGSFSFDIHHRYSDVVRGILPVDGLPEEGTVDY 60

Query: 61  YAAMVRRDHFVRARRLAEDRPPLTFLSGNETIRLNALGLYGFLFPALRRGYSGNAGGFVF 120
           Y AMVRRD  +  RRL+ED+PPLTFL GNET+R+N LG   +    +             
Sbjct: 61  YTAMVRRDILLHGRRLSEDQPPLTFLLGNETVRVNPLGFLHYAKVTVGTPKVSYLVALDT 120

Query: 121 GGTGHWQRFVLV------------VKFNIYSPKNSSTSKEVPCSSSLCSHADQCFPPGDR 180
           G    W     V             +FNIYSP NSSTSKEVPCSSSLC HA+QCF P D 
Sbjct: 121 GSDLFWLPCDCVNCVTEYNTSEGRARFNIYSPSNSSTSKEVPCSSSLCQHANQCFSPSDP 180

Query: 181 CPYHVSYLSDNTSSTGYLVEDILHLTTNDGPSKPVKASITLGCGKDQSGAFLANAAPNGL 240
           CPY VSYLSDNTSSTGYLVEDILHL TNDG SKPV A+ITLGCG+DQSGAFL+ AAPNGL
Sbjct: 181 CPYKVSYLSDNTSSTGYLVEDILHLATNDGRSKPVNANITLGCGRDQSGAFLSTAAPNGL 240

Query: 241 FGLGIENVSVPSILANQGLTSNSFSLCFGAGGMGRIEFGDKGSPGQSETPFNLGHKHPTY 300
           FGLGIE+VSVPSILAN+GLTSNSFSLCFG  GMGRIEFGDKGSPGQSETPFN+GH+HPTY
Sbjct: 241 FGLGIESVSVPSILANEGLTSNSFSLCFGPRGMGRIEFGDKGSPGQSETPFNVGHRHPTY 300

Query: 301 NISITQISVGGNVSNLGFSAVFDSGTSFTYLNDPAYSLIAGKFASMVEEKPFTMNSDLPF 360
           NISITQ++VGGNVSNL F+AVFDSGTSFTYLN+PAYSLIA KF SMV+EK +  N D+PF
Sbjct: 301 NISITQLNVGGNVSNLDFAAVFDSGTSFTYLNEPAYSLIADKFDSMVDEKRYMGNLDIPF 360

Query: 361 ENCYELSPNQTTFTYPVMNLTMKGGAHFFIHRPVVLVALQTTR-LYCLAISRSDNINIIG 420
           ENCYELSPNQT F YPVMNLTM+GGAHFFI+ P+V++A + T   YCLAISRSDNINIIG
Sbjct: 361 ENCYELSPNQTKFRYPVMNLTMEGGAHFFINHPIVVLASEATSWFYCLAISRSDNINIIG 420

Query: 421 QNFMTGYHIVFDREKMVLGWKGSNCTGYEDENMNNLPVSPSSAPAA--APGTAINPQANS 480
           QNFMTGYHIVFDREKMVLGWK SNCTGYED   NNLP+ PS+AP A  APGT I P+ANS
Sbjct: 421 QNFMTGYHIVFDREKMVLGWKESNCTGYEDVKTNNLPIHPSTAPTAAPAPGTTIKPEANS 480

Query: 481 EMNNTSQTIEKPRPANNSSNLVRSVILTFLMTVVPFLVFV 506
           +MNN+S+T++KPR ANNS  L  SVIL  LM  VPFL FV
Sbjct: 481 QMNNSSETLDKPRSANNSKKLGSSVILRLLMAGVPFLGFV 520

BLAST of Spg021327 vs. ExPASy TrEMBL
Match: A0A5D3CJM4 (Aspartyl protease family protein 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G00510 PE=3 SV=1)

HSP 1 Score: 711.4 bits (1835), Expect = 2.6e-201
Identity = 375/523 (71.70%), Postives = 409/523 (78.20%), Query Frame = 0

Query: 1   MASPSSLF-LTLCVFFSIFSFFSHSCHALQSFRFKIHHRYSDAVRGILPVDGLPEEGTFD 60
           M S SS F LTLC F SIF+F SH  H   SF F IHH YS AVR ILP    P+EGT D
Sbjct: 1   MPSSSSTFSLTLCFFLSIFTFISHFSHVFGSFTFNIHHLYSPAVRQILPFHSFPDEGTLD 60

Query: 61  YYAAMVRRDHFVRARRL--AEDRPPLTFLSGNETIRLNALGLYGFLFPALRRGYSGNAGG 120
           YYAAMVR DHFV +RRL   +D PPLTFLSGNET+R++ L   GFL+ A     +     
Sbjct: 61  YYAAMVRTDHFVHSRRLGQVQDHPPLTFLSGNETLRISPL---GFLYYAEVTVGTPGVPY 120

Query: 121 FVFGGTG---HWQRFVLV------------VKFNIYSPKNSSTSKEVPCSSSLCSHADQC 180
            V   TG    W     V            V FNIYSP NSSTSKEV CSSSLCSH DQC
Sbjct: 121 LVALDTGSDLFWLPCDCVNCITGLNTTQGPVNFNIYSPNNSSTSKEVQCSSSLCSHPDQC 180

Query: 181 FPPGDRCPYHVSYLSDNTSSTGYLVEDILHLTTNDGPSKPVKASITLGCGKDQSGAFLAN 240
             P D CPY VSYLSDNTSSTGYLVEDILHLTTND  SKPV A+ITLGCGKDQSGAFL++
Sbjct: 181 SLPSDTCPYQVSYLSDNTSSTGYLVEDILHLTTNDVQSKPVNATITLGCGKDQSGAFLSS 240

Query: 241 AAPNGLFGLGIENVSVPSILANQGLTSNSFSLCFGAGGMGRIEFGDKGSPGQSETPFNLG 300
           AAPNGLFGLGIENVSVPSILAN GL SNSFSLCFG   MGRIEFGDKGSP Q+ETPFNLG
Sbjct: 241 AAPNGLFGLGIENVSVPSILANAGLISNSFSLCFGPARMGRIEFGDKGSPDQNETPFNLG 300

Query: 301 HKHPTYNISITQISVGGNVSNLGFSAVFDSGTSFTYLNDPAYSLIAGKFASMVEEKPFTM 360
            +HPTYN+SITQI+VGG++SNL  + +FDSGTSFTYLNDPAYSL A KF SMVEEK +TM
Sbjct: 301 RRHPTYNVSITQIAVGGHISNLDVAVIFDSGTSFTYLNDPAYSLFADKFDSMVEEKRYTM 360

Query: 361 NSDLPFENCYELSPNQTTFTYPVMNLTMKGGAHFFIHRPVVLVALQTTRLYCLAISRSDN 420
           NSD+PFENCYELSP+QTTFTYPVMNLTMKGG HF I+ P+VL++ Q+ RL+CLAI+RSD+
Sbjct: 361 NSDIPFENCYELSPDQTTFTYPVMNLTMKGGGHFVINHPIVLLSAQSKRLFCLAIARSDS 420

Query: 421 INIIGQNFMTGYHIVFDREKMVLGWKGSNCTGYEDENMNNLPVSPSSAPAAAPGTAINPQ 480
           INIIGQNFMTGYHIVFDREKMVLGWK SNCTGYEDEN NNLPV PS  PAAAPGT I PQ
Sbjct: 421 INIIGQNFMTGYHIVFDREKMVLGWKESNCTGYEDENTNNLPVGPSPTPAAAPGTTIKPQ 480

Query: 481 ANSEMNNTSQTIEKPRPANNSSNLVRSVILTFLMTVVPFLVFV 506
           ANS +NNT+QTIEKPRP N SS L  SVILTFLM VV FL+FV
Sbjct: 481 ANSNVNNTTQTIEKPRPTNISSKLPTSVILTFLMPVVTFLLFV 520

BLAST of Spg021327 vs. ExPASy TrEMBL
Match: A0A5A7UHC9 (Aspartyl protease family protein 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold344G00490 PE=3 SV=1)

HSP 1 Score: 706.8 bits (1823), Expect = 6.4e-200
Identity = 375/524 (71.56%), Postives = 409/524 (78.05%), Query Frame = 0

Query: 1   MASPSSLF-LTLCVFFSIFSFFSHSCHALQSFRFKIHHRYSDAVRGILPVDGLPEEGTFD 60
           M S SS F LTLC F SIF+F SH  H   SF F IHH YS AVR ILP    P+EGT D
Sbjct: 1   MPSSSSTFSLTLCFFLSIFTFISHFSHVFGSFTFNIHHLYSPAVRQILPFHSFPDEGTLD 60

Query: 61  YYAAMVRRDHFVRARRL--AEDRPPLTFLSGNETIRLNALGLYGFLFPALRRGYSGNAGG 120
           YYAAMVR DHFV +RRL   +D PPLTFLSGNET+R++ L   GFL+ A     +     
Sbjct: 61  YYAAMVRTDHFVHSRRLGQVQDHPPLTFLSGNETLRISPL---GFLYYAEVTVGTPGVPY 120

Query: 121 FVFGGTG---HWQRFVLV------------VKFNIYSPKNSSTSKEVPCSSSLCSHADQC 180
            V   TG    W     V            V FNIYSP NSSTSKEV CSSSLCSH DQC
Sbjct: 121 LVALDTGSDLFWLPCDCVNCITGLNTTQGPVNFNIYSPNNSSTSKEVQCSSSLCSHPDQC 180

Query: 181 FPPGDRCPYHVSYLSDNTSSTGYLVEDILHLTTNDGPSKPVKASITLGCGKDQSGAFLAN 240
             P D CPY VSYLSDNTSSTGYLVEDILHLTTND  SKPV A+ITLGCGKDQSGAFL++
Sbjct: 181 SLPSDTCPYQVSYLSDNTSSTGYLVEDILHLTTNDVQSKPVNATITLGCGKDQSGAFLSS 240

Query: 241 AAPNGLFGLGIENVSVPSILANQGLTSNSFSLCFGAGGMGRIEFGDKGSPGQSETPFNLG 300
           AAPNGLFGLGIENVSVPSILAN GL SNSFSLCFG   MGRIEFGDKGSP Q+ETPFNLG
Sbjct: 241 AAPNGLFGLGIENVSVPSILANAGLISNSFSLCFGPARMGRIEFGDKGSPDQNETPFNLG 300

Query: 301 HKHPTYNISITQISVGGNVSNLGFSAVFDSGTSFTYLNDPAYSLIAGKFASMVEEKPFTM 360
            +HPTYN+SITQI+VGG++SNL  + +FDSGTSFTYLNDPAYSL A KF SMVEEK +TM
Sbjct: 301 RRHPTYNVSITQIAVGGHISNLDVAVIFDSGTSFTYLNDPAYSLFADKFDSMVEEKRYTM 360

Query: 361 NSDLPFENCYELSPNQTTFTYPVMNLTMKGGAHFFIHRPVVLVALQTTRLYCLAISRSDN 420
           NSD+PFENCYELSP+QTTFTYPVMNLTMKGG HF I+ P+VL++ Q+ RL+CLAI+RSD+
Sbjct: 361 NSDIPFENCYELSPDQTTFTYPVMNLTMKGGGHFVINHPIVLLSAQSKRLFCLAIARSDS 420

Query: 421 INIIGQNFMTGYHIVFDREKMVLGWKGSNCTGYEDENMNNLPVSPSSAPAAAPG-TAINP 480
           INIIGQNFMTGYHIVFDREKMVLGWK SNCTGYEDEN NNLPV PS  PAAAPG T I P
Sbjct: 421 INIIGQNFMTGYHIVFDREKMVLGWKESNCTGYEDENTNNLPVGPSPTPAAAPGTTTIKP 480

Query: 481 QANSEMNNTSQTIEKPRPANNSSNLVRSVILTFLMTVVPFLVFV 506
           QANS +NNT+QTIEKPRP N SS L  SVILTFLM VV FL+FV
Sbjct: 481 QANSNVNNTTQTIEKPRPTNISSKLPTSVILTFLMPVVTFLLFV 521

BLAST of Spg021327 vs. ExPASy TrEMBL
Match: A0A0A0L3M6 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G002590 PE=3 SV=1)

HSP 1 Score: 705.3 bits (1819), Expect = 1.9e-199
Identity = 371/522 (71.07%), Postives = 408/522 (78.16%), Query Frame = 0

Query: 2   ASPSSLFLTLCVFFSIFSFFSHSCHALQSFRFKIHHRYSDAVRGILPVDGLPEEGTFDYY 61
           +S S+  LTLC FF IF F SH  H   SF F IHH YS AVR ILP    P+EGT DYY
Sbjct: 6   SSSSTFSLTLCFFFFIFIFISHFSHVFGSFTFNIHHLYSPAVRQILPFHSFPDEGTLDYY 65

Query: 62  AAMVRRDHFVRARRL--AEDRPPLTFLSGNETIRLNALGLYGFLFPALRRGYSGNAGGFV 121
           AAMVR DHFV +RRL   +D  PLTFLSGNET+R++ L   GFL+ A     +      V
Sbjct: 66  AAMVRTDHFVHSRRLGQVQDHRPLTFLSGNETLRISPL---GFLYYAEVTVGTPGVPYLV 125

Query: 122 FGGTG---HWQRFVLV------------VKFNIYSPKNSSTSKEVPCSSSLCSHADQCFP 181
              TG    W     V            V FNIYSP NSSTSKEV CSSSLCSH DQC  
Sbjct: 126 ALDTGSDLFWLPCDCVNCITGLNTTQGPVNFNIYSPNNSSTSKEVQCSSSLCSHLDQCSS 185

Query: 182 PGDRCPYHVSYLSDNTSSTGYLVEDILHLTTNDGPSKPVKASITLGCGKDQSGAFLANAA 241
           P D CPY VSYLSDNTSSTGYLVEDILHLTTND  SKPV A ITLGCGKDQSGAFL++AA
Sbjct: 186 PSDTCPYQVSYLSDNTSSTGYLVEDILHLTTNDVQSKPVNARITLGCGKDQSGAFLSSAA 245

Query: 242 PNGLFGLGIENVSVPSILANQGLTSNSFSLCFGAGGMGRIEFGDKGSPGQSETPFNLGHK 301
           PNGLFGLGIENVSVPSILAN GL SNSFSLCFG   MGRIEFGDKGSPGQ+ETPFNLG +
Sbjct: 246 PNGLFGLGIENVSVPSILANAGLISNSFSLCFGPARMGRIEFGDKGSPGQNETPFNLGRR 305

Query: 302 HPTYNISITQISVGGNVSNLGFSAVFDSGTSFTYLNDPAYSLIAGKFASMVEEKPFTMNS 361
           HPTYN+SITQI VGG++S+L  + +FDSGTSFTYLNDPAYSL A KFASMVEEK FTMNS
Sbjct: 306 HPTYNVSITQIGVGGHISDLDVAVIFDSGTSFTYLNDPAYSLFADKFASMVEEKQFTMNS 365

Query: 362 DLPFENCYELSPNQTTFTYPVMNLTMKGGAHFFIHRPVVLVALQTTRLYCLAISRSDNIN 421
           D+PFENCYELSPNQTTFTYP+MNLTMKGG HF I+ P+VL++ ++ RL+CLAI+RSD+IN
Sbjct: 366 DIPFENCYELSPNQTTFTYPLMNLTMKGGGHFVINHPIVLISTESKRLFCLAIARSDSIN 425

Query: 422 IIGQNFMTGYHIVFDREKMVLGWKGSNCTGYEDENMNNLPVSPSSAPAAAPG-TAINPQA 481
           IIGQNFMTGYHIVFDREKMVLGWK SNCTGYEDEN NNLPV P+  PAAAPG TAI PQA
Sbjct: 426 IIGQNFMTGYHIVFDREKMVLGWKESNCTGYEDENTNNLPVGPTPTPAAAPGTTAIKPQA 485

Query: 482 NSEMNNTSQTIEKPRPANNSSNLVRSVILTFLMTVVPFLVFV 506
           NS +NNT+QTIEKPRP+N SS L  SVILTFL++VV FL FV
Sbjct: 486 NSNINNTTQTIEKPRPSNISSKLPTSVILTFLISVVTFLHFV 524

BLAST of Spg021327 vs. TAIR 10
Match: AT2G17760.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 444.1 bits (1141), Expect = 1.5e-124
Identity = 239/486 (49.18%), Postives = 321/486 (66.05%), Query Frame = 0

Query: 7   LFLTLCVFFSIFSFFSHSCHALQSFRFKIHHRYSDAVRGILPVDGLPEEGTFDYYAAMVR 66
           LFL L +  +  S+    C     F F+ HHR+SD V G+LP DGLP   +  YY  M  
Sbjct: 10  LFLGLLILLA-SSWVLDRCEGFGEFGFEFHHRFSDQVVGVLPGDGLPNRDSSKYYRVMAH 69

Query: 67  RDHFVRARRLA-EDRPPLTFLSGNETIRLNALGLYGFL-----FPALRRGYSGNAGGFVF 126
           RD  +R RRLA ED+  +TF  GNET+R++ALG   +       P+     + + G  +F
Sbjct: 70  RDRLIRGRRLANEDQSLVTFSDGNETVRVDALGFLHYANVTVGTPSDWFMVALDTGSDLF 129

Query: 127 GGTGHWQRFVLVVK--------FNIYSPKNSSTSKEVPCSSSLCSHADQCFPPGDRCPYH 186
                    V  +K         NIYSP  SSTS +VPC+S+LC+  D+C  P   CPY 
Sbjct: 130 WLPCDCTNCVRELKAPGGSSLDLNIYSPNASSTSTKVPCNSTLCTRGDRCASPESDCPYQ 189

Query: 187 VSYLSDNTSSTGYLVEDILHLTTNDGPSKPVKASITLGCGKDQSGAFLANAAPNGLFGLG 246
           + YLS+ TSSTG LVED+LHL +ND  SK + A +T GCG+ Q+G F   AAPNGLFGLG
Sbjct: 190 IRYLSNGTSSTGVLVEDVLHLVSNDKSSKAIPARVTFGCGQVQTGVFHDGAAPNGLFGLG 249

Query: 247 IENVSVPSILANQGLTSNSFSLCFGAGGMGRIEFGDKGSPGQSETPFNLGHKHPTYNISI 306
           +E++SVPS+LA +G+ +NSFS+CFG  G GRI FGDKGS  Q ETP N+   HPTYNI++
Sbjct: 250 LEDISVPSVLAKEGIAANSFSMCFGNDGAGRISFGDKGSVDQRETPLNIRQPHPTYNITV 309

Query: 307 TQISVGGNVSNLGFSAVFDSGTSFTYLNDPAYSLIAGKFASMVEEKPF-TMNSDLPFENC 366
           T+ISVGGN  +L F AVFDSGTSFTYL D AY+LI+  F S+  +K + T +S+LPFE C
Sbjct: 310 TKISVGGNTGDLEFDAVFDSGTSFTYLTDAAYTLISESFNSLALDKRYQTTDSELPFEYC 369

Query: 367 YELSPNQTTFTYPVMNLTMKGGAHFFIHRPVVLVALQTTRLYCLAISRSDNINIIGQNFM 426
           Y LSPN+ +F YP +NLTMKGG+ + ++ P+V++ ++ T +YCLAI + ++I+IIGQNFM
Sbjct: 370 YALSPNKDSFQYPAVNLTMKGGSSYPVYHPLVVIPMKDTDVYCLAIMKIEDISIIGQNFM 429

Query: 427 TGYHIVFDREKMVLGWKGSNC-TGYEDENMNNLPVSPSSAPAAAPGTAINPQAN---SEM 474
           TGY +VFDREK++LGWK S+C TG  + +   LP + SS+ A  P ++ +P+A    S+ 
Sbjct: 430 TGYRVVFDREKLILGWKESDCYTG--ETSARTLPSNRSSSSARPPASSFDPEATNIPSQR 489

BLAST of Spg021327 vs. TAIR 10
Match: AT4G35880.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 388.3 bits (996), Expect = 9.6e-108
Identity = 208/490 (42.45%), Postives = 307/490 (62.65%), Query Frame = 0

Query: 24  SCHALQSFRFKIHHRYSDAVRGILPVDG----LPEEGTFDYYAAMVRRDHFVRARRL--- 83
           SC+  + F F++HHR+SD V+      G     P +G+F+Y+ A+V RD  +R RRL   
Sbjct: 23  SCNG-RIFTFEMHHRFSDEVKQWSDSTGRFAKFPPKGSFEYFNALVLRDWLIRGRRLSES 82

Query: 84  -AEDRPPLTFLSGNETIRLNALGLYGFL-----FPALRRGYSGNAGGFVFG--------G 143
            +E    LTF  GN T R+++LG   +       P +R   + + G  +F          
Sbjct: 83  ESESESSLTFSDGNSTSRISSLGFLHYTTVKLGTPGMRFMVALDTGSDLFWVPCDCGKCA 142

Query: 144 TGHWQRFVLVVKFNIYSPKNSSTSKEVPCSSSLCSHADQCFPPGDRCPYHVSYLSDNTSS 203
                 +    + +IY+PK S+T+K+V C++SLC+  +QC      CPY VSY+S  TS+
Sbjct: 143 PTEGATYASEFELSIYNPKVSTTNKKVTCNNSLCAQRNQCLGTFSTCPYMVSYVSAQTST 202

Query: 204 TGYLVEDILHLTTNDGPSKPVKASITLGCGKDQSGAFLANAAPNGLFGLGIENVSVPSIL 263
           +G L+ED++HLTT D   + V+A +T GCG+ QSG+FL  AAPNGLFGLG+E +SVPS+L
Sbjct: 203 SGILMEDVMHLTTEDKNPERVEAYVTFGCGQVQSGSFLDIAAPNGLFGLGMEKISVPSVL 262

Query: 264 ANQGLTSNSFSLCFGAGGMGRIEFGDKGSPGQSETPFNLGHKHPTYNISITQISVGGNVS 323
           A +GL ++SFS+CFG  G+GRI FGDKGS  Q ETPFNL   HP YNI++T++ VG  + 
Sbjct: 263 AREGLVADSFSMCFGHDGVGRISFGDKGSSDQEETPFNLNPSHPNYNITVTRVRVGTTLI 322

Query: 324 NLGFSAVFDSGTSFTYLNDPAYSLIAGKFASMVEEKPFTMNSDLPFENCYELSPNQTTFT 383
           +  F+A+FD+GTSFTYL DP Y+ ++  F S  ++K  + +S +PFE CY++S +     
Sbjct: 323 DDEFTALFDTGTSFTYLVDPMYTTVSESFHSQAQDKRHSPDSRIPFEYCYDMSNDANASL 382

Query: 384 YPVMNLTMKGGAHFFIHRPVVLVALQTTRLYCLAISRSDNINIIGQNFMTGYHIVFDREK 443
            P ++LTMKG +HF I+ P+++++ +   +YCLAI +S  +NIIGQN+MTGY +VFDREK
Sbjct: 383 IPSLSLTMKGNSHFTINDPIIVISTEGELVYCLAIVKSSELNIIGQNYMTGYRVVFDREK 442

Query: 444 MVLGWKGSNCTGYEDENMNNLPVSPSSAPAAAPGTAINPQAN-SEMNNTSQTIEKPRPAN 492
           +VL WK  +C   E+ N      + ++A A A    I    N SE++ T+QTI K   +N
Sbjct: 443 LVLAWKKFDCYDIEETNTTVAGTNKTAAVAPAMAAGIKTHNNSSELHKTNQTISK---SN 502

BLAST of Spg021327 vs. TAIR 10
Match: AT3G51330.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 344.0 bits (881), Expect = 2.1e-94
Identity = 203/511 (39.73%), Postives = 296/511 (57.93%), Query Frame = 0

Query: 25  CHALQSFRFKIHHRYSDAVRGILPVDGL-PEEGTFDYYAAMVRRDHFVRARRLA--EDRP 84
           C A   F F++HH +SD V+  L +D L PE+G+ +Y+  + +RD  +R R LA   +  
Sbjct: 23  CEASGKFSFEVHHMFSDRVKQSLGLDDLVPEKGSLEYFKVLAQRDRLIRGRGLASNNEET 82

Query: 85  PLTFLSGNETIRLNALGLYGFLFPALRRGYSGNAGGFVFGGTGHW------QRFVLVVK- 144
           P+TF+ GN TI ++ LG   +   ++    +        G    W         +  +K 
Sbjct: 83  PITFMRGNRTISIDLLGFLHYANVSVGTPATWFLVALDTGSDLFWLPCNCGSTCIRDLKE 142

Query: 145 --------FNIYSPKNSSTSKEVPCSSSLCSHADQCFPPGDRCPYHVSYLSDNTSSTGYL 204
                    N+YSP  SSTS  + CS   C  + +C  P   CPY + YLS +T +TG L
Sbjct: 143 VGLSQSRPLNLYSPNTSSTSSSIRCSDDRCFGSSRCSSPASSCPYQIQYLSKDTFTTGTL 202

Query: 205 VEDILHLTTNDGPSKPVKASITLGCGKDQSGAFLANAAPNGLFGLGIENVSVPSILANQG 264
            ED+LHL T D   +PVKA+ITLGCGK+Q+G   ++AA NGL GLG+++ SVPSILA   
Sbjct: 203 FEDVLHLVTEDEGLEPVKANITLGCGKNQTGFLQSSAAVNGLLGLGLKDYSVPSILAKAK 262

Query: 265 LTSNSFSLCFG--AGGMGRIEFGDKGSPGQSETPFNLGHKHPTYNISITQISVGGNVSNL 324
           +T+NSFS+CFG     +GRI FGDKG   Q ETP       PTY +S+T++SVGG+   +
Sbjct: 263 ITANSFSMCFGNIIDVVGRISFGDKGYTDQMETPLLPTEPSPTYAVSVTEVSVGGDAVGV 322

Query: 325 GFSAVFDSGTSFTYLNDPAYSLIAGKFASMVEEKPFTMNSDLPFENCYELSPNQTTFTYP 384
              A+FD+GTSFT+L +P Y LI   F   V +K   ++ +LPFE CY+LSPN+TT  +P
Sbjct: 323 QLLALFDTGTSFTHLLEPEYGLITKAFDDHVTDKRRPIDPELPFEFCYDLSPNKTTILFP 382

Query: 385 VMNLTMKGGAHFFIHRPVVLVALQ-TTRLYCLAISRSDN--INIIGQNFMTGYHIVFDRE 444
            + +T +GG+  F+  P+ +V  +  + +YCL I +S +  INIIGQNFM+GY IVFDRE
Sbjct: 383 RVAMTFEGGSQMFLRNPLFIVWNEDNSAMYCLGILKSVDFKINIIGQNFMSGYRIVFDRE 442

Query: 445 KMVLGWKGSNCTGYEDENMNNL--PVSPSSAPAAAPGTAINPQANSEMNNTSQTIEKPRP 504
           +M+LGWK S+C  +EDE++ +   P   + AP+ +  T +          T   I+    
Sbjct: 443 RMILGWKRSDC--FEDESLESTTPPPPETEAPSPSASTPLPSLLPPPAAATPPQIDPRNS 502

Query: 505 ANNS-----SNLVRSVILTFLMTVVPFLVFV 506
             NS     +NLV   + + L+ ++P L F+
Sbjct: 503 TRNSGTGTAANLV--PLASQLLLLLPLLAFL 529

BLAST of Spg021327 vs. TAIR 10
Match: AT3G51350.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 318.5 bits (815), Expect = 9.4e-87
Identity = 187/502 (37.25%), Postives = 276/502 (54.98%), Query Frame = 0

Query: 7   LFLTLCVFFSIFSFFSHSCHALQSFRFKIHHRYSDAVRGILPV-DGLPEEGTFDYYAAMV 66
           +F+ L V    + F    C A   F F++HH +SD+V+  L + D +PE+G+ +Y+  + 
Sbjct: 7   VFVLLSVLVVCWGF--ERCEATGKFGFEVHHIFSDSVKQSLGLGDLVPEQGSLEYFKVLA 66

Query: 67  RRDHFVRARRLA--EDRPPLTFLSGNETIRLNALGLYGFLFPALRRGYSGNAGGFVFGGT 126
            RD  +R R LA   D  P+TF  GN T+ +  LG   +   ++    S        G  
Sbjct: 67  HRDRLIRGRGLASNNDETPITFDGGNLTVSVKLLGSLYYANVSVGTPPSSFLVALDTGSD 126

Query: 127 GHWQ---------------RFVLVVKFNIYSPKNSSTSKEVPCSSSLCSHADQCFPPGDR 186
             W                     V  N+Y+P  S+TS  + CS   C  + +C  P   
Sbjct: 127 LFWLPCNCGTTCIRDLEDIGVPQSVPLNLYTPNASTTSSSIRCSDKRCFGSKKCSSPSSI 186

Query: 187 CPYHVSYLSDNTSSTGYLVEDILHLTTNDGPSKPVKASITLGCGKDQSGAFLANAAPNGL 246
           CPY +SY S++T + G L++D+LHL T D    PVKA++TLGCG+ Q+G F  N + NG+
Sbjct: 187 CPYQISY-SNSTGTKGTLLQDVLHLATEDENLTPVKANVTLGCGQKQTGLFQRNNSVNGV 246

Query: 247 FGLGIENVSVPSILANQGLTSNSFSLCFG--AGGMGRIEFGDKGSPGQSETPFNLGHKHP 306
            GLGI+  SVPS+LA   +T+NSFS+CFG   G +GRI FGD+G   Q ETPF       
Sbjct: 247 LGLGIKGYSVPSLLAKANITANSFSMCFGRVIGNVGRISFGDRGYTDQEETPFISVAPST 306

Query: 307 TYNISITQISVGGNVSNLGFSAVFDSGTSFTYLNDPAYSLIAGKFASMVEEKPFTMNSDL 366
            Y ++I+ +SV G+  ++   A FD+G+SFT+L +PAY ++   F  +VE++   ++ +L
Sbjct: 307 AYGVNISGVSVAGDPVDIRLFAKFDTGSSFTHLREPAYGVLTKSFDELVEDRRRPVDPEL 366

Query: 367 PFENCYELSPNQTTFTYPVMNLTMKGGAHFFIHRPVVLVALQTTR-LYCLAISRSD--NI 426
           PFE CY+LSPN TT  +P++ +T  GG+   ++ P      Q    +YCL + +S    I
Sbjct: 367 PFEFCYDLSPNATTIQFPLVEMTFIGGSKIILNNPFFTARTQEGNVMYCLGVLKSVGLKI 426

Query: 427 NIIGQNFMTGYHIVFDREKMVLGWKGSNCTGYEDENMNNLPVSPSSAPAAAPGTAINPQA 486
           N+IGQNF+ GY IVFDRE+M+LGWK S C  +EDE++ +    P    A AP  +  P  
Sbjct: 427 NVIGQNFVAGYRIVFDRERMILGWKQSLC--FEDESLESTTPPPPEVEAPAPSVSA-PPP 486

BLAST of Spg021327 vs. TAIR 10
Match: AT3G51340.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 293.1 bits (749), Expect = 4.2e-79
Identity = 188/538 (34.94%), Postives = 277/538 (51.49%), Query Frame = 0

Query: 7   LFLTLCVFFSIFSFFSHSCHALQSFRFKIHHRYSDAVRGILPVDGL-PEEGTFDYYAAMV 66
           +F+ L +   IF +    C A   F F++HH +SD V+  L  D L PE G+ +Y+  + 
Sbjct: 7   VFVLLSMLVLIF-WGLERCEASGKFSFEVHHMFSDVVKQTLGFDDLVPENGSLEYFKVLA 66

Query: 67  RRDHFVRARRLA--EDRPPLTFLSGNETIRLNALGLYGFLFPALRRGYSGNAGGFVFGGT 126
            RD F+R R LA   +  PLT +  N T+ LN LG   +   +L    +        G  
Sbjct: 67  HRDRFIRGRGLASNNEETPLTSIGSNLTLALNFLGFLHYANVSLGTPATWFLVALDTGSD 126

Query: 127 GHW---------------QRFVLVVKFNIYSPKNSSTSKEVPCSSSLCSHADQCFPPGDR 186
             W                RF   V  N+Y+P  S+TS  + CS   C  + +C  P   
Sbjct: 127 LFWLPCNCGTTCIHDLKDARFSESVPLNLYTPNASTTSSSIRCSDKRCFGSGKCSSPESI 186

Query: 187 CPYHVSYLSDNTSSTGYLVEDILHLTTNDGPSKPVKASITLGCGKDQSGAFLANAAPNGL 246
           CPY ++ LS NT +TG L++D+LHL T D   KPV A++TLGCG++Q+GAF  + A NG+
Sbjct: 187 CPYQIA-LSSNTVTTGTLLQDVLHLVTEDEDLKPVNANVTLGCGQNQTGAFQTDIAVNGV 246

Query: 247 FGLGIENVSVPSILANQGLTSNSFSLCFG--AGGMGRIEFGDKGSPGQSETPFNLGHKHP 306
            GL ++  SVPS+LA   +T+NSFS+CFG     +GRI FGDKG   Q ETP        
Sbjct: 247 LGLSMKEYSVPSLLAKANITANSFSMCFGRIISVVGRISFGDKGYTDQEETPLVSLETST 306

Query: 307 TYNISITQISVGGNVSNLGFSAVFDSGTSFTYLNDPAYSLIAGKFASMVEEKPFTMNSDL 366
            Y +++T +SVGG   ++   A+FD+G+SFT L + AY +    F  ++E+K   ++ D 
Sbjct: 307 AYGVNVTGVSVGGVPVDVPLFALFDTGSSFTLLLESAYGVFTKAFDDLMEDKRRPVDPDF 366

Query: 367 PFENCYEL-------------------SPNQTTFTYPVMNLTMKGGAHFFIHRPVVLVAL 426
           PFE CY+L                   +P +  F + + N + +           V  + 
Sbjct: 367 PFEFCYDLREEHLNSDARPRHMQSKCYNPCRDDFRWRIQNDSQES----------VSYSN 426

Query: 427 QTTRLYCLAISRSDNINIIGQNFMTGYHIVFDREKMVLGWKGSNCTGYEDENMNNLPVSP 486
           + T++YCL I +S N+NIIGQN M+G+ IVFDRE+M+LGWK SNC  +EDE++ +    P
Sbjct: 427 EGTKMYCLGILKSINLNIIGQNLMSGHRIVFDRERMILGWKQSNC--FEDESLASESPPP 486

Query: 487 SSAPAAAPGTAINPQANSEMNNTSQTIEKPRPANNSSNLVRSVILTFLMTVVPFLVFV 506
               A  P  +  P A S    T       R +        S +   L+ ++P L F+
Sbjct: 487 PEIEAPPPSVSTPPPAASATPPTIDPRNSTRNSGTGGAANLSPLAAQLLFLLPLLAFL 530

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038906112.12.8e-21073.90aspartyl protease family protein 1 [Benincasa hispida][more]
XP_022971529.19.1e-20973.46aspartyl protease family protein 1-like isoform X1 [Cucurbita maxima][more]
XP_023540295.11.2e-20571.92aspartyl protease family protein 1-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022943747.18.0e-20571.92aspartyl protease family protein 1-like isoform X1 [Cucurbita moschata][more]
KAG6596257.13.7e-20272.05Aspartyl protease family protein 1, partial [Cucurbita argyrosperma subsp. soror... [more]
Match NameE-valueIdentityDescription
Q8VYV92.1e-12349.18Aspartyl protease family protein 1 OS=Arabidopsis thaliana OX=3702 GN=APF1 PE=2 ... [more]
Q9LX204.9e-5634.55Aspartic proteinase-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At5g10080 ... [more]
Q4V3D27.9e-2227.60Aspartic proteinase 36 OS=Arabidopsis thaliana OX=3702 GN=A36 PE=1 SV=1[more]
Q9M9A81.3e-1625.60Aspartyl protease APCB1 OS=Arabidopsis thaliana OX=3702 GN=APCB1 PE=1 SV=1[more]
Q766C23.2e-1524.53Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A6J1I8U24.4e-20973.46aspartyl protease family protein 1-like isoform X1 OS=Cucurbita maxima OX=3661 G... [more]
A0A6J1FTX03.9e-20571.92aspartyl protease family protein 1-like isoform X1 OS=Cucurbita moschata OX=3662... [more]
A0A5D3CJM42.6e-20171.70Aspartyl protease family protein 1-like OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A5A7UHC96.4e-20071.56Aspartyl protease family protein 1-like OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A0A0L3M61.9e-19971.07Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G00259... [more]
Match NameE-valueIdentityDescription
AT2G17760.11.5e-12449.18Eukaryotic aspartyl protease family protein [more]
AT4G35880.19.6e-10842.45Eukaryotic aspartyl protease family protein [more]
AT3G51330.12.1e-9439.73Eukaryotic aspartyl protease family protein [more]
AT3G51350.19.4e-8737.25Eukaryotic aspartyl protease family protein [more]
AT3G51340.14.2e-7934.94Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 124..268
e-value: 1.0E-28
score: 102.7
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 271..435
e-value: 1.8E-32
score: 114.5
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 134..432
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 288..428
e-value: 9.3E-18
score: 64.5
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 137..268
e-value: 1.2E-25
score: 90.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 447..480
NoneNo IPR availablePANTHERPTHR13683:SF826ASPARTYL PROTEASE FAMILY PROTEIN 1coord: 10..100
coord: 131..445
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 10..100
coord: 131..445
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 308..319
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 103..428
score: 24.54563

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg021327.1Spg021327.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004190 aspartic-type endopeptidase activity